id sid tid token lemma pos cord-258379-v3lceirh 1 1 key key JJ cord-258379-v3lceirh 1 2 : : : cord-258379-v3lceirh 1 3 cord-258379-v3lceirh cord-258379-v3lceirh JJ cord-258379-v3lceirh 1 4 authors author NNS cord-258379-v3lceirh 1 5 : : : cord-258379-v3lceirh 1 6 Liu Liu NNP cord-258379-v3lceirh 1 7 , , , cord-258379-v3lceirh 1 8 D. D. NNP cord-258379-v3lceirh 1 9 X. X. NNP cord-258379-v3lceirh 1 10 ; ; : cord-258379-v3lceirh 1 11 Inglis Inglis NNP cord-258379-v3lceirh 1 12 , , , cord-258379-v3lceirh 1 13 S. S. NNP cord-258379-v3lceirh 1 14 C. C. NNP cord-258379-v3lceirh 1 15 title title NN cord-258379-v3lceirh 1 16 : : : cord-258379-v3lceirh 1 17 Association Association NNP cord-258379-v3lceirh 1 18 of of IN cord-258379-v3lceirh 1 19 the the DT cord-258379-v3lceirh 1 20 infectious infectious JJ cord-258379-v3lceirh 1 21 bronchitis bronchitis NN cord-258379-v3lceirh 1 22 virus virus NN cord-258379-v3lceirh 1 23 3c 3c NNP cord-258379-v3lceirh 1 24 protein protein NN cord-258379-v3lceirh 1 25 with with IN cord-258379-v3lceirh 1 26 the the DT cord-258379-v3lceirh 1 27 virion virion NN cord-258379-v3lceirh 1 28 envelope envelope NN cord-258379-v3lceirh 1 29 date date NN cord-258379-v3lceirh 1 30 : : : cord-258379-v3lceirh 1 31 1991 1991 CD cord-258379-v3lceirh 1 32 - - SYM cord-258379-v3lceirh 1 33 12 12 CD cord-258379-v3lceirh 1 34 - - SYM cord-258379-v3lceirh 1 35 31 31 CD cord-258379-v3lceirh 1 36 journal journal NN cord-258379-v3lceirh 1 37 : : : cord-258379-v3lceirh 2 1 Virology virology NN cord-258379-v3lceirh 2 2 DOI DOI NNP cord-258379-v3lceirh 2 3 : : : cord-258379-v3lceirh 2 4 10.1016/0042 10.1016/0042 CD cord-258379-v3lceirh 2 5 - - SYM cord-258379-v3lceirh 2 6 6822(91)90572-s 6822(91)90572-s CD cord-258379-v3lceirh 2 7 sha sha NNP cord-258379-v3lceirh 2 8 : : : cord-258379-v3lceirh 2 9 61d6a568f97488b71a7ad350bbdfe784a4e224e3 61d6a568f97488b71a7ad350bbdfe784a4e224e3 CD cord-258379-v3lceirh 2 10 doc_id doc_id CD cord-258379-v3lceirh 2 11 : : : cord-258379-v3lceirh 2 12 258379 258379 CD cord-258379-v3lceirh 2 13 cord_uid cord_uid NNS cord-258379-v3lceirh 2 14 : : : cord-258379-v3lceirh 3 1 v3lceirh v3lceirh NNP cord-258379-v3lceirh 4 1 Abstract Abstract NNP cord-258379-v3lceirh 4 2 A a DT cord-258379-v3lceirh 4 3 highly highly RB cord-258379-v3lceirh 4 4 purified purify VBN cord-258379-v3lceirh 4 5 radiolabeled radiolabeled JJ cord-258379-v3lceirh 4 6 preparation preparation NN cord-258379-v3lceirh 4 7 of of IN cord-258379-v3lceirh 4 8 the the DT cord-258379-v3lceirh 4 9 coronavirus coronavirus NN cord-258379-v3lceirh 4 10 infectious infectious JJ cord-258379-v3lceirh 4 11 bronchitis bronchitis NN cord-258379-v3lceirh 4 12 virus virus NN cord-258379-v3lceirh 4 13 ( ( -LRB- cord-258379-v3lceirh 4 14 IBV IBV NNP cord-258379-v3lceirh 4 15 ) ) -RRB- cord-258379-v3lceirh 4 16 was be VBD cord-258379-v3lceirh 4 17 analyzed analyze VBN cord-258379-v3lceirh 4 18 , , , cord-258379-v3lceirh 4 19 by by IN cord-258379-v3lceirh 4 20 immunoprecipitation immunoprecipitation NN cord-258379-v3lceirh 4 21 with with IN cord-258379-v3lceirh 4 22 monospecific monospecific JJ cord-258379-v3lceirh 4 23 antisera antisera NN cord-258379-v3lceirh 4 24 , , , cord-258379-v3lceirh 4 25 for for IN cord-258379-v3lceirh 4 26 the the DT cord-258379-v3lceirh 4 27 presence presence NN cord-258379-v3lceirh 4 28 of of IN cord-258379-v3lceirh 4 29 a a DT cord-258379-v3lceirh 4 30 series series NN cord-258379-v3lceirh 4 31 of of IN cord-258379-v3lceirh 4 32 small small JJ cord-258379-v3lceirh 4 33 virus virus NN cord-258379-v3lceirh 4 34 proteins protein NNS cord-258379-v3lceirh 4 35 recently recently RB cord-258379-v3lceirh 4 36 identified identify VBN cord-258379-v3lceirh 4 37 as as IN cord-258379-v3lceirh 4 38 the the DT cord-258379-v3lceirh 4 39 products product NNS cord-258379-v3lceirh 4 40 of of IN cord-258379-v3lceirh 4 41 I -PRON- PRP cord-258379-v3lceirh 4 42 BV BV NNP cord-258379-v3lceirh 4 43 mRNAs mrnas CD cord-258379-v3lceirh 4 44 3 3 CD cord-258379-v3lceirh 4 45 and and CC cord-258379-v3lceirh 4 46 5 5 CD cord-258379-v3lceirh 4 47 . . . cord-258379-v3lceirh 5 1 One one CD cord-258379-v3lceirh 5 2 of of IN cord-258379-v3lceirh 5 3 these these DT cord-258379-v3lceirh 5 4 , , , cord-258379-v3lceirh 5 5 3c 3c NNP cord-258379-v3lceirh 5 6 , , , cord-258379-v3lceirh 5 7 a a DT cord-258379-v3lceirh 5 8 12.4 12.4 CD cord-258379-v3lceirh 5 9 K K NNP cord-258379-v3lceirh 5 10 protein protein NN cord-258379-v3lceirh 5 11 encoded encode VBN cord-258379-v3lceirh 5 12 by by IN cord-258379-v3lceirh 5 13 the the DT cord-258379-v3lceirh 5 14 third third JJ cord-258379-v3lceirh 5 15 open open JJ cord-258379-v3lceirh 5 16 reading reading NN cord-258379-v3lceirh 5 17 frame frame NN cord-258379-v3lceirh 5 18 of of IN cord-258379-v3lceirh 5 19 the the DT cord-258379-v3lceirh 5 20 tricistronic tricistronic NNP cord-258379-v3lceirh 5 21 mRNA3 mrna3 NN cord-258379-v3lceirh 5 22 was be VBD cord-258379-v3lceirh 5 23 clearly clearly RB cord-258379-v3lceirh 5 24 detectable detectable JJ cord-258379-v3lceirh 5 25 and and CC cord-258379-v3lceirh 5 26 was be VBD cord-258379-v3lceirh 5 27 found find VBN cord-258379-v3lceirh 5 28 to to TO cord-258379-v3lceirh 5 29 cofractionate cofractionate VB cord-258379-v3lceirh 5 30 with with IN cord-258379-v3lceirh 5 31 virion virion NN cord-258379-v3lceirh 5 32 envelope envelope NN cord-258379-v3lceirh 5 33 proteins protein NNS cord-258379-v3lceirh 5 34 on on IN cord-258379-v3lceirh 5 35 detergent detergent NN cord-258379-v3lceirh 5 36 disruption disruption NN cord-258379-v3lceirh 5 37 of of IN cord-258379-v3lceirh 5 38 virus virus NN cord-258379-v3lceirh 5 39 particles particle NNS cord-258379-v3lceirh 5 40 . . . cord-258379-v3lceirh 6 1 These these DT cord-258379-v3lceirh 6 2 results result NNS cord-258379-v3lceirh 6 3 , , , cord-258379-v3lceirh 6 4 together together RB cord-258379-v3lceirh 6 5 with with IN cord-258379-v3lceirh 6 6 the the DT cord-258379-v3lceirh 6 7 hydrophobic hydrophobic JJ cord-258379-v3lceirh 6 8 nature nature NN cord-258379-v3lceirh 6 9 of of IN cord-258379-v3lceirh 6 10 3c 3c NNP cord-258379-v3lceirh 6 11 and and CC cord-258379-v3lceirh 6 12 its -PRON- PRP$ cord-258379-v3lceirh 6 13 previously previously RB cord-258379-v3lceirh 6 14 demonstrated demonstrate VBN cord-258379-v3lceirh 6 15 association association NN cord-258379-v3lceirh 6 16 with with IN cord-258379-v3lceirh 6 17 the the DT cord-258379-v3lceirh 6 18 membranes membrane NNS cord-258379-v3lceirh 6 19 of of IN cord-258379-v3lceirh 6 20 infected infected JJ cord-258379-v3lceirh 6 21 cells cell NNS cord-258379-v3lceirh 6 22 , , , cord-258379-v3lceirh 6 23 suggest suggest VBP cord-258379-v3lceirh 6 24 strongly strongly RB cord-258379-v3lceirh 6 25 that that IN cord-258379-v3lceirh 6 26 3c 3c NNP cord-258379-v3lceirh 6 27 represents represent VBZ cord-258379-v3lceirh 6 28 a a DT cord-258379-v3lceirh 6 29 new new JJ cord-258379-v3lceirh 6 30 virion virion NN cord-258379-v3lceirh 6 31 envelope envelope NN cord-258379-v3lceirh 6 32 protein protein NN cord-258379-v3lceirh 6 33 , , , cord-258379-v3lceirh 6 34 which which WDT cord-258379-v3lceirh 6 35 may may MD cord-258379-v3lceirh 6 36 have have VB cord-258379-v3lceirh 6 37 counterparts counterpart NNS cord-258379-v3lceirh 6 38 in in IN cord-258379-v3lceirh 6 39 other other JJ cord-258379-v3lceirh 6 40 coronaviruses coronaviruse NNS cord-258379-v3lceirh 6 41 . . . cord-258379-v3lceirh 7 1 Infectious infectious JJ cord-258379-v3lceirh 7 2 bronchitis bronchitis NN cord-258379-v3lceirh 7 3 virus virus NN cord-258379-v3lceirh 7 4 ( ( -LRB- cord-258379-v3lceirh 7 5 IBV IBV NNP cord-258379-v3lceirh 7 6 ) ) -RRB- cord-258379-v3lceirh 7 7 is be VBZ cord-258379-v3lceirh 7 8 the the DT cord-258379-v3lceirh 7 9 prototype prototype NN cord-258379-v3lceirh 7 10 virus virus NN cord-258379-v3lceirh 7 11 of of IN cord-258379-v3lceirh 7 12 the the DT cord-258379-v3lceirh 7 13 Coronaviridae Coronaviridae NNP cord-258379-v3lceirh 7 14 , , , cord-258379-v3lceirh 7 15 a a DT cord-258379-v3lceirh 7 16 family family NN cord-258379-v3lceirh 7 17 of of IN cord-258379-v3lceirh 7 18 viruses virus NNS cord-258379-v3lceirh 7 19 containing contain VBG cord-258379-v3lceirh 7 20 a a DT cord-258379-v3lceirh 7 21 large large JJ cord-258379-v3lceirh 7 22 single single JJ cord-258379-v3lceirh 7 23 - - HYPH cord-258379-v3lceirh 7 24 stranded strand VBN cord-258379-v3lceirh 7 25 RNA RNA NNP cord-258379-v3lceirh 7 26 genome genome NN cord-258379-v3lceirh 7 27 of of IN cord-258379-v3lceirh 7 28 positive positive JJ cord-258379-v3lceirh 7 29 sense sense NN cord-258379-v3lceirh 7 30 . . . cord-258379-v3lceirh 8 1 The the DT cord-258379-v3lceirh 8 2 morphology morphology NN cord-258379-v3lceirh 8 3 of of IN cord-258379-v3lceirh 8 4 the the DT cord-258379-v3lceirh 8 5 virion virion NN cord-258379-v3lceirh 8 6 is be VBZ cord-258379-v3lceirh 8 7 characterised characterise VBN cord-258379-v3lceirh 8 8 by by IN cord-258379-v3lceirh 8 9 a a DT cord-258379-v3lceirh 8 10 " " `` cord-258379-v3lceirh 8 11 corona corona NN cord-258379-v3lceirh 8 12 " " '' cord-258379-v3lceirh 8 13 of of IN cord-258379-v3lceirh 8 14 widely widely RB cord-258379-v3lceirh 8 15 spaced spaced JJ cord-258379-v3lceirh 8 16 bulbous bulbous JJ cord-258379-v3lceirh 8 17 projections projection NNS cord-258379-v3lceirh 8 18 which which WDT cord-258379-v3lceirh 8 19 consist consist VBP cord-258379-v3lceirh 8 20 of of IN cord-258379-v3lceirh 8 21 the the DT cord-258379-v3lceirh 8 22 virus virus NN cord-258379-v3lceirh 8 23 spike spike NN cord-258379-v3lceirh 8 24 glycoprotein glycoprotein NN cord-258379-v3lceirh 8 25 ( ( -LRB- cord-258379-v3lceirh 8 26 S s NN cord-258379-v3lceirh 8 27 ) ) -RRB- cord-258379-v3lceirh 8 28 ( ( -LRB- cord-258379-v3lceirh 8 29 27 27 CD cord-258379-v3lceirh 8 30 ) ) -RRB- cord-258379-v3lceirh 8 31 . . . cord-258379-v3lceirh 9 1 This this DT cord-258379-v3lceirh 9 2 S S NNP cord-258379-v3lceirh 9 3 protein protein NN cord-258379-v3lceirh 9 4 is be VBZ cord-258379-v3lceirh 9 5 composed compose VBN cord-258379-v3lceirh 9 6 of of IN cord-258379-v3lceirh 9 7 two two CD cord-258379-v3lceirh 9 8 glycopolypeptides glycopolypeptide NNS cord-258379-v3lceirh 9 9 , , , cord-258379-v3lceirh 9 10 gp90 gp90 NNP cord-258379-v3lceirh 9 11 and and CC cord-258379-v3lceirh 9 12 gp84 gp84 NNP cord-258379-v3lceirh 9 13 , , , cord-258379-v3lceirh 9 14 which which WDT cord-258379-v3lceirh 9 15 are be VBP cord-258379-v3lceirh 9 16 produced produce VBN cord-258379-v3lceirh 9 17 by by IN cord-258379-v3lceirh 9 18 post post JJ cord-258379-v3lceirh 9 19 - - JJ cord-258379-v3lceirh 9 20 translational translational JJ cord-258379-v3lceirh 9 21 cleavage cleavage NN cord-258379-v3lceirh 9 22 of of IN cord-258379-v3lceirh 9 23 a a DT cord-258379-v3lceirh 9 24 precursor precursor NN cord-258379-v3lceirh 9 25 , , , cord-258379-v3lceirh 9 26 gp155 gp155 NNP cord-258379-v3lceirh 9 27 ( ( -LRB- cord-258379-v3lceirh 9 28 7 7 CD cord-258379-v3lceirh 9 29 , , , cord-258379-v3lceirh 9 30 8 8 CD cord-258379-v3lceirh 9 31 , , , cord-258379-v3lceirh 9 32 25 25 CD cord-258379-v3lceirh 9 33 ) ) -RRB- cord-258379-v3lceirh 9 34 . . . cord-258379-v3lceirh 10 1 In in IN cord-258379-v3lceirh 10 2 addition addition NN cord-258379-v3lceirh 10 3 to to IN cord-258379-v3lceirh 10 4 the the DT cord-258379-v3lceirh 10 5 spike spike NN cord-258379-v3lceirh 10 6 protein protein NN cord-258379-v3lceirh 10 7 , , , cord-258379-v3lceirh 10 8 only only RB cord-258379-v3lceirh 10 9 two two CD cord-258379-v3lceirh 10 10 other other JJ cord-258379-v3lceirh 10 11 major major JJ cord-258379-v3lceirh 10 12 virion virion NN cord-258379-v3lceirh 10 13 structural structural JJ cord-258379-v3lceirh 10 14 proteins protein NNS cord-258379-v3lceirh 10 15 are be VBP cord-258379-v3lceirh 10 16 currently currently RB cord-258379-v3lceirh 10 17 recognized recognize VBN cord-258379-v3lceirh 10 18 . . . cord-258379-v3lceirh 11 1 These these DT cord-258379-v3lceirh 11 2 are be VBP cord-258379-v3lceirh 11 3 the the DT cord-258379-v3lceirh 11 4 nucleocapsid nucleocapsid NNP cord-258379-v3lceirh 11 5 protein protein NNP cord-258379-v3lceirh 11 6 ( ( -LRB- cord-258379-v3lceirh 11 7 N N NNP cord-258379-v3lceirh 11 8 ) ) -RRB- cord-258379-v3lceirh 11 9 , , , cord-258379-v3lceirh 11 10 which which WDT cord-258379-v3lceirh 11 11 is be VBZ cord-258379-v3lceirh 11 12 a a DT cord-258379-v3lceirh 11 13 5 5 CD cord-258379-v3lceirh 11 14 1 1 CD cord-258379-v3lceirh 11 15 K k NN cord-258379-v3lceirh 11 16 phosphoprotein phosphoprotein NN cord-258379-v3lceirh 11 17 found find VBN cord-258379-v3lceirh 11 18 in in IN cord-258379-v3lceirh 11 19 close close JJ cord-258379-v3lceirh 11 20 association association NN cord-258379-v3lceirh 11 21 with with IN cord-258379-v3lceirh 11 22 the the DT cord-258379-v3lceirh 11 23 genomic genomic JJ cord-258379-v3lceirh 11 24 RNA RNA NNP cord-258379-v3lceirh 11 25 ( ( -LRB- cord-258379-v3lceirh 11 26 26 26 CD cord-258379-v3lceirh 11 27 ) ) -RRB- cord-258379-v3lceirh 11 28 and and CC cord-258379-v3lceirh 11 29 the the DT cord-258379-v3lceirh 11 30 membrane membrane NN cord-258379-v3lceirh 11 31 protein protein NN cord-258379-v3lceirh 11 32 ( ( -LRB- cord-258379-v3lceirh 11 33 M M NNP cord-258379-v3lceirh 11 34 ) ) -RRB- cord-258379-v3lceirh 11 35 , , , cord-258379-v3lceirh 11 36 an an DT cord-258379-v3lceirh 11 37 integral integral JJ cord-258379-v3lceirh 11 38 envelope envelope NN cord-258379-v3lceirh 11 39 protein protein NN cord-258379-v3lceirh 11 40 which which WDT cord-258379-v3lceirh 11 41 appears appear VBZ cord-258379-v3lceirh 11 42 as as IN cord-258379-v3lceirh 11 43 a a DT cord-258379-v3lceirh 11 44 heterogeneous heterogeneous JJ cord-258379-v3lceirh 11 45 collection collection NN cord-258379-v3lceirh 11 46 of of IN cord-258379-v3lceirh 11 47 molecules molecule NNS cord-258379-v3lceirh 11 48 differing differ VBG cord-258379-v3lceirh 11 49 in in IN cord-258379-v3lceirh 11 50 their -PRON- PRP$ cord-258379-v3lceirh 11 51 extent extent NN cord-258379-v3lceirh 11 52 of of IN cord-258379-v3lceirh 11 53 glycosylation glycosylation NN cord-258379-v3lceirh 11 54 ( ( -LRB- cord-258379-v3lceirh 11 55 ~23 ~23 XX cord-258379-v3lceirh 11 56 , , , cord-258379-v3lceirh 11 57 gp28 gp28 NNP cord-258379-v3lceirh 11 58 , , , cord-258379-v3lceirh 11 59 gp31 gp31 NNP cord-258379-v3lceirh 11 60 , , , cord-258379-v3lceirh 11 61 and and CC cord-258379-v3lceirh 11 62 gp36 gp36 NN cord-258379-v3lceirh 11 63 ) ) -RRB- cord-258379-v3lceirh 11 64 ( ( -LRB- cord-258379-v3lceirh 11 65 8 8 CD cord-258379-v3lceirh 11 66 , , , cord-258379-v3lceirh 11 67 24 24 CD cord-258379-v3lceirh 11 68 ) ) -RRB- cord-258379-v3lceirh 11 69 . . . cord-258379-v3lceirh 12 1 Boursnell Boursnell NNP cord-258379-v3lceirh 12 2 and and CC cord-258379-v3lceirh 12 3 colleagues colleague NNS cord-258379-v3lceirh 12 4 ( ( -LRB- cord-258379-v3lceirh 12 5 4 4 LS cord-258379-v3lceirh 12 6 ) ) -RRB- cord-258379-v3lceirh 12 7 have have VBP cord-258379-v3lceirh 12 8 deduced deduce VBN cord-258379-v3lceirh 12 9 the the DT cord-258379-v3lceirh 12 10 complete complete JJ cord-258379-v3lceirh 12 11 sequence sequence NN cord-258379-v3lceirh 12 12 of of IN cord-258379-v3lceirh 12 13 IBV IBV NNP cord-258379-v3lceirh 12 14 genomic genomic JJ cord-258379-v3lceirh 12 15 RNA rna NN cord-258379-v3lceirh 12 16 through through IN cord-258379-v3lceirh 12 17 cloning cloning NN cord-258379-v3lceirh 12 18 of of IN cord-258379-v3lceirh 12 19 cDNA cdna NN cord-258379-v3lceirh 12 20 . . . cord-258379-v3lceirh 13 1 Their -PRON- PRP$ cord-258379-v3lceirh 13 2 results result NNS cord-258379-v3lceirh 13 3 indicated indicate VBD cord-258379-v3lceirh 13 4 that that IN cord-258379-v3lceirh 13 5 the the DT cord-258379-v3lceirh 13 6 IBV IBV NNP cord-258379-v3lceirh 13 7 genome genome NN cord-258379-v3lceirh 13 8 is be VBZ cord-258379-v3lceirh 13 9 27.6 27.6 CD cord-258379-v3lceirh 13 10 kb kb NNP cord-258379-v3lceirh 13 11 in in IN cord-258379-v3lceirh 13 12 length length NN cord-258379-v3lceirh 13 13 with with IN cord-258379-v3lceirh 13 14 at at RB cord-258379-v3lceirh 13 15 least least JJS cord-258379-v3lceirh 13 16 ten ten CD cord-258379-v3lceirh 13 17 open open JJ cord-258379-v3lceirh 13 18 reading reading NN cord-258379-v3lceirh 13 19 frames frame NNS cord-258379-v3lceirh 13 20 ( ( -LRB- cord-258379-v3lceirh 13 21 ORFs ORFs NNP cord-258379-v3lceirh 13 22 ) ) -RRB- cord-258379-v3lceirh 13 23 , , , cord-258379-v3lceirh 13 24 of of IN cord-258379-v3lceirh 13 25 which which WDT cord-258379-v3lceirh 13 26 only only RB cord-258379-v3lceirh 13 27 three three CD cord-258379-v3lceirh 13 28 were be VBD cord-258379-v3lceirh 13 29 known know VBN cord-258379-v3lceirh 13 30 certainly certainly RB cord-258379-v3lceirh 13 31 to to TO cord-258379-v3lceirh 13 32 encode encode VB cord-258379-v3lceirh 13 33 virus virus NN cord-258379-v3lceirh 13 34 proteins protein NNS cord-258379-v3lceirh 13 35 ( ( -LRB- cord-258379-v3lceirh 13 36 the the DT cord-258379-v3lceirh 13 37 S S NNP cord-258379-v3lceirh 13 38 , , , cord-258379-v3lceirh 13 39 N N NNP cord-258379-v3lceirh 13 40 , , , cord-258379-v3lceirh 13 41 and and CC cord-258379-v3lceirh 13 42 M M NNP cord-258379-v3lceirh 13 43 genes gene NNS cord-258379-v3lceirh 13 44 ) ) -RRB- cord-258379-v3lceirh 13 45 . . . cord-258379-v3lceirh 14 1 The the DT cord-258379-v3lceirh 14 2 seven seven CD cord-258379-v3lceirh 14 3 unassigned unassigned JJ cord-258379-v3lceirh 14 4 ORFs orf NNS cord-258379-v3lceirh 14 5 were be VBD cord-258379-v3lceirh 14 6 clustered cluster VBN cord-258379-v3lceirh 14 7 in in IN cord-258379-v3lceirh 14 8 three three CD cord-258379-v3lceirh 14 9 groups group NNS cord-258379-v3lceirh 14 10 on on IN cord-258379-v3lceirh 14 11 the the DT cord-258379-v3lceirh 14 12 genome genome NN cord-258379-v3lceirh 14 13 - - HYPH cord-258379-v3lceirh 14 14 two two CD cord-258379-v3lceirh 14 15 very very RB cord-258379-v3lceirh 14 16 large large JJ cord-258379-v3lceirh 14 17 ORFs orfs NN cord-258379-v3lceirh 14 18 at at IN cord-258379-v3lceirh 14 19 the the DT cord-258379-v3lceirh 14 20 5 5 CD cord-258379-v3lceirh 14 21 ' ' NN cord-258379-v3lceirh 14 22 end end NN cord-258379-v3lceirh 14 23 of of IN cord-258379-v3lceirh 14 24 the the DT cord-258379-v3lceirh 14 25 RNA RNA NNP cord-258379-v3lceirh 14 26 ( ( -LRB- cord-258379-v3lceirh 14 27 1 1 CD cord-258379-v3lceirh 14 28 a a NN cord-258379-v3lceirh 14 29 and and CC cord-258379-v3lceirh 14 30 1 1 CD cord-258379-v3lceirh 14 31 b b NN cord-258379-v3lceirh 14 32 ) ) -RRB- cord-258379-v3lceirh 14 33 , , , cord-258379-v3lceirh 14 34 three three CD cord-258379-v3lceirh 14 35 small small JJ cord-258379-v3lceirh 14 36 ones one NNS cord-258379-v3lceirh 14 37 between between IN cord-258379-v3lceirh 14 38 the the DT cord-258379-v3lceirh 14 39 S S NNP cord-258379-v3lceirh 14 40 and and CC cord-258379-v3lceirh 14 41 N n CD cord-258379-v3lceirh 14 42 genes gene NNS cord-258379-v3lceirh 14 43 ( ( -LRB- cord-258379-v3lceirh 14 44 3a 3a NNP cord-258379-v3lceirh 14 45 , , , cord-258379-v3lceirh 14 46 3b 3b NNP cord-258379-v3lceirh 14 47 , , , cord-258379-v3lceirh 14 48 and and CC cord-258379-v3lceirh 14 49 3c 3c NNP cord-258379-v3lceirh 14 50 ) ) -RRB- cord-258379-v3lceirh 14 51 , , , cord-258379-v3lceirh 14 52 and and CC cord-258379-v3lceirh 14 53 two two CD cord-258379-v3lceirh 14 54 small small JJ cord-258379-v3lceirh 14 55 ones one NNS cord-258379-v3lceirh 14 56 between between IN cord-258379-v3lceirh 14 57 the the DT cord-258379-v3lceirh 14 58 M M NNP cord-258379-v3lceirh 14 59 and and CC cord-258379-v3lceirh 14 60 the the DT cord-258379-v3lceirh 14 61 N n CD cord-258379-v3lceirh 14 62 genes gene NNS cord-258379-v3lceirh 14 63 ( ( -LRB- cord-258379-v3lceirh 14 64 5a 5a NN cord-258379-v3lceirh 14 65 and and CC cord-258379-v3lceirh 14 66 5b 5b CD cord-258379-v3lceirh 14 67 ) ) -RRB- cord-258379-v3lceirh 14 68 . . . cord-258379-v3lceirh 15 1 Recently recently RB cord-258379-v3lceirh 15 2 through through IN cord-258379-v3lceirh 15 3 expression expression NN cord-258379-v3lceirh 15 4 of of IN cord-258379-v3lceirh 15 5 nucleotide nucleotide JJ cord-258379-v3lceirh 15 6 sequences sequence NNS cord-258379-v3lceirh 15 7 from from IN cord-258379-v3lceirh 15 8 these these DT cord-258379-v3lceirh 15 9 unassigned unassigned JJ cord-258379-v3lceirh 15 10 ORFs orfs NN cord-258379-v3lceirh 15 11 as as IN cord-258379-v3lceirh 15 12 bacterial bacterial JJ cord-258379-v3lceirh 15 13 fusion fusion NN cord-258379-v3lceirh 15 14 proteins protein NNS cord-258379-v3lceirh 15 15 , , , cord-258379-v3lceirh 15 16 and and CC cord-258379-v3lceirh 15 17 production production NN cord-258379-v3lceirh 15 18 of of IN cord-258379-v3lceirh 15 19 monospecific monospecific JJ cord-258379-v3lceirh 15 20 antisera antisera NN cord-258379-v3lceirh 15 21 against against IN cord-258379-v3lceirh 15 22 the the DT cord-258379-v3lceirh 15 23 bacterial bacterial JJ cord-258379-v3lceirh 15 24 products product NNS cord-258379-v3lceirh 15 25 , , , cord-258379-v3lceirh 15 26 we -PRON- PRP cord-258379-v3lceirh 15 27 have have VBP cord-258379-v3lceirh 15 28 been be VBN cord-258379-v3lceirh 15 29 able able JJ cord-258379-v3lceirh 15 30 to to TO cord-258379-v3lceirh 15 31 establish establish VB cord-258379-v3lceirh 15 32 posi- posi- XX cord-258379-v3lceirh 15 33 ' ' '' cord-258379-v3lceirh 16 1 To to TO cord-258379-v3lceirh 16 2 whom whom WP cord-258379-v3lceirh 16 3 reprint reprint VB cord-258379-v3lceirh 16 4 requests request NNS cord-258379-v3lceirh 16 5 should should MD cord-258379-v3lceirh 16 6 be be VB cord-258379-v3lceirh 16 7 addressed address VBN cord-258379-v3lceirh 16 8 . . . cord-258379-v3lceirh 17 1 tively tively RB cord-258379-v3lceirh 17 2 the the DT cord-258379-v3lceirh 17 3 coding code VBG cord-258379-v3lceirh 17 4 function function NN cord-258379-v3lceirh 17 5 of of IN cord-258379-v3lceirh 17 6 several several JJ cord-258379-v3lceirh 17 7 of of IN cord-258379-v3lceirh 17 8 these these DT cord-258379-v3lceirh 17 9 ORFs orf NNS cord-258379-v3lceirh 17 10 . . . cord-258379-v3lceirh 18 1 It -PRON- PRP cord-258379-v3lceirh 18 2 now now RB cord-258379-v3lceirh 18 3 appears appear VBZ cord-258379-v3lceirh 18 4 that that IN cord-258379-v3lceirh 18 5 the the DT cord-258379-v3lceirh 18 6 3a 3a NN cord-258379-v3lceirh 18 7 , , , cord-258379-v3lceirh 18 8 3b 3b NNP cord-258379-v3lceirh 18 9 , , , cord-258379-v3lceirh 18 10 and and CC cord-258379-v3lceirh 18 11 3c 3c NNP cord-258379-v3lceirh 18 12 ORFs ORFs NNP cord-258379-v3lceirh 18 13 encode encode VBP cord-258379-v3lceirh 18 14 polypeptides polypeptide NNS cord-258379-v3lceirh 18 15 in in IN cord-258379-v3lceirh 18 16 virus virus NN cord-258379-v3lceirh 18 17 - - HYPH cord-258379-v3lceirh 18 18 infected infect VBN cord-258379-v3lceirh 18 19 cells cell NNS cord-258379-v3lceirh 18 20 ( ( -LRB- cord-258379-v3lceirh 18 21 17 17 CD cord-258379-v3lceirh 18 22 , , , cord-258379-v3lceirh 18 23 23 23 CD cord-258379-v3lceirh 18 24 ) ) -RRB- cord-258379-v3lceirh 18 25 and and CC cord-258379-v3lceirh 18 26 that that IN cord-258379-v3lceirh 18 27 the the DT cord-258379-v3lceirh 18 28 5a 5a CD cord-258379-v3lceirh 18 29 and and CC cord-258379-v3lceirh 18 30 5b 5b NN cord-258379-v3lceirh 18 31 ORFs ORFs NNP cord-258379-v3lceirh 18 32 also also RB cord-258379-v3lceirh 18 33 represent represent VBP cord-258379-v3lceirh 18 34 functional functional JJ cord-258379-v3lceirh 18 35 genes gene NNS cord-258379-v3lceirh 18 36 ( ( -LRB- cord-258379-v3lceirh 18 37 18 18 CD cord-258379-v3lceirh 18 38 ) ) -RRB- cord-258379-v3lceirh 18 39 . . . cord-258379-v3lceirh 19 1 It -PRON- PRP cord-258379-v3lceirh 19 2 was be VBD cord-258379-v3lceirh 19 3 therefore therefore RB cord-258379-v3lceirh 19 4 of of IN cord-258379-v3lceirh 19 5 interest interest NN cord-258379-v3lceirh 19 6 to to TO cord-258379-v3lceirh 19 7 examine examine VB cord-258379-v3lceirh 19 8 whether whether IN cord-258379-v3lceirh 19 9 any any DT cord-258379-v3lceirh 19 10 of of IN cord-258379-v3lceirh 19 11 these these DT cord-258379-v3lceirh 19 12 newly newly RB cord-258379-v3lceirh 19 13 identified identify VBN cord-258379-v3lceirh 19 14 proteins protein NNS cord-258379-v3lceirh 19 15 are be VBP cord-258379-v3lceirh 19 16 associated associate VBN cord-258379-v3lceirh 19 17 with with IN cord-258379-v3lceirh 19 18 the the DT cord-258379-v3lceirh 19 19 virus virus NN cord-258379-v3lceirh 19 20 particle particle NN cord-258379-v3lceirh 19 21 . . . cord-258379-v3lceirh 20 1 Although although IN cord-258379-v3lceirh 20 2 their -PRON- PRP$ cord-258379-v3lceirh 20 3 function function NN cord-258379-v3lceirh 20 4 is be VBZ cord-258379-v3lceirh 20 5 as as RB cord-258379-v3lceirh 20 6 yet yet RB cord-258379-v3lceirh 20 7 unknown unknown JJ cord-258379-v3lceirh 20 8 , , , cord-258379-v3lceirh 20 9 certain certain JJ cord-258379-v3lceirh 20 10 of of IN cord-258379-v3lceirh 20 11 the the DT cord-258379-v3lceirh 20 12 polypeptides polypeptide NNS cord-258379-v3lceirh 20 13 , , , cord-258379-v3lceirh 20 14 particularly particularly RB cord-258379-v3lceirh 20 15 the the DT cord-258379-v3lceirh 20 16 12.4 12.4 CD cord-258379-v3lceirh 20 17 K K NNP cord-258379-v3lceirh 20 18 3c 3c NNP cord-258379-v3lceirh 20 19 and and CC cord-258379-v3lceirh 20 20 7.5 7.5 CD cord-258379-v3lceirh 20 21 K K NNP cord-258379-v3lceirh 20 22 5a 5a CD cord-258379-v3lceirh 20 23 polypeptides polypeptide NNS cord-258379-v3lceirh 20 24 , , , cord-258379-v3lceirh 20 25 possess possess VBP cord-258379-v3lceirh 20 26 hydrophobic hydrophobic JJ cord-258379-v3lceirh 20 27 sequences sequence NNS cord-258379-v3lceirh 20 28 characteristic characteristic JJ cord-258379-v3lceirh 20 29 of of IN cord-258379-v3lceirh 20 30 integral integral JJ cord-258379-v3lceirh 20 31 membrane membrane NN cord-258379-v3lceirh 20 32 proteins protein NNS cord-258379-v3lceirh 20 33 ( ( -LRB- cord-258379-v3lceirh 20 34 2 2 CD cord-258379-v3lceirh 20 35 , , , cord-258379-v3lceirh 20 36 3 3 CD cord-258379-v3lceirh 20 37 ) ) -RRB- cord-258379-v3lceirh 20 38 and and CC cord-258379-v3lceirh 20 39 we -PRON- PRP cord-258379-v3lceirh 20 40 have have VBP cord-258379-v3lceirh 20 41 shown show VBN cord-258379-v3lceirh 20 42 previously previously RB cord-258379-v3lceirh 20 43 that that IN cord-258379-v3lceirh 20 44 3c 3c CD cord-258379-v3lceirh 20 45 can can MD cord-258379-v3lceirh 20 46 be be VB cord-258379-v3lceirh 20 47 found find VBN cord-258379-v3lceirh 20 48 in in IN cord-258379-v3lceirh 20 49 association association NN cord-258379-v3lceirh 20 50 with with IN cord-258379-v3lceirh 20 51 IBV IBV NNP cord-258379-v3lceirh 20 52 - - HYPH cord-258379-v3lceirh 20 53 infected infect VBN cord-258379-v3lceirh 20 54 cell cell NN cord-258379-v3lceirh 20 55 membranes membrane NNS cord-258379-v3lceirh 20 56 ( ( -LRB- cord-258379-v3lceirh 20 57 23 23 CD cord-258379-v3lceirh 20 58 ) ) -RRB- cord-258379-v3lceirh 20 59 . . . cord-258379-v3lceirh 21 1 We -PRON- PRP cord-258379-v3lceirh 21 2 were be VBD cord-258379-v3lceirh 21 3 therefore therefore RB cord-258379-v3lceirh 21 4 particularly particularly RB cord-258379-v3lceirh 21 5 interested interested JJ cord-258379-v3lceirh 21 6 in in IN cord-258379-v3lceirh 21 7 the the DT cord-258379-v3lceirh 21 8 possibility possibility NN cord-258379-v3lceirh 21 9 that that IN cord-258379-v3lceirh 21 10 these these DT cord-258379-v3lceirh 21 11 proteins protein NNS cord-258379-v3lceirh 21 12 might may MD cord-258379-v3lceirh 21 13 represent represent VB cord-258379-v3lceirh 21 14 previously previously RB cord-258379-v3lceirh 21 15 unidentified unidentified JJ cord-258379-v3lceirh 21 16 virion virion NN cord-258379-v3lceirh 21 17 envelope envelope NN cord-258379-v3lceirh 21 18 proteins protein NNS cord-258379-v3lceirh 21 19 . . . cord-258379-v3lceirh 22 1 We -PRON- PRP cord-258379-v3lceirh 22 2 report report VBP cord-258379-v3lceirh 22 3 here here RB cord-258379-v3lceirh 22 4 that that IN cord-258379-v3lceirh 22 5 the the DT cord-258379-v3lceirh 22 6 3c 3c NNP cord-258379-v3lceirh 22 7 protein protein NN cord-258379-v3lceirh 22 8 is be VBZ cord-258379-v3lceirh 22 9 indeed indeed RB cord-258379-v3lceirh 22 10 virion virion NN cord-258379-v3lceirh 22 11 - - HYPH cord-258379-v3lceirh 22 12 associated associate VBN cord-258379-v3lceirh 22 13 and and CC cord-258379-v3lceirh 22 14 that that IN cord-258379-v3lceirh 22 15 it -PRON- PRP cord-258379-v3lceirh 22 16 cofractionates cofractionate VBZ cord-258379-v3lceirh 22 17 with with IN cord-258379-v3lceirh 22 18 other other JJ cord-258379-v3lceirh 22 19 virion virion NN cord-258379-v3lceirh 22 20 envelope envelope NN cord-258379-v3lceirh 22 21 proteins protein NNS cord-258379-v3lceirh 22 22 on on IN cord-258379-v3lceirh 22 23 detergent detergent NN cord-258379-v3lceirh 22 24 disruption disruption NN cord-258379-v3lceirh 22 25 of of IN cord-258379-v3lceirh 22 26 virus virus NN cord-258379-v3lceirh 22 27 particles particle NNS cord-258379-v3lceirh 22 28 . . . cord-258379-v3lceirh 23 1 Confluent confluent NN cord-258379-v3lceirh 23 2 monolayers monolayer NNS cord-258379-v3lceirh 23 3 of of IN cord-258379-v3lceirh 23 4 primary primary JJ cord-258379-v3lceirh 23 5 chicken chicken NN cord-258379-v3lceirh 23 6 kidney kidney NN cord-258379-v3lceirh 23 7 cells cell NNS cord-258379-v3lceirh 23 8 were be VBD cord-258379-v3lceirh 23 9 prepared prepare VBN cord-258379-v3lceirh 23 10 from from IN cord-258379-v3lceirh 23 11 2-to 2-to CD cord-258379-v3lceirh 23 12 3-week 3-week JJ cord-258379-v3lceirh 23 13 - - HYPH cord-258379-v3lceirh 23 14 old old JJ cord-258379-v3lceirh 23 15 birds bird NNS cord-258379-v3lceirh 23 16 as as IN cord-258379-v3lceirh 23 17 previously previously RB cord-258379-v3lceirh 23 18 described describe VBN cord-258379-v3lceirh 23 19 ( ( -LRB- cord-258379-v3lceirh 23 20 23 23 CD cord-258379-v3lceirh 23 21 mm mm NN cord-258379-v3lceirh 23 22 diameter diameter NN cord-258379-v3lceirh 23 23 ) ) -RRB- cord-258379-v3lceirh 23 24 of of IN cord-258379-v3lceirh 23 25 IBV IBV NNP cord-258379-v3lceirh 23 26 - - HYPH cord-258379-v3lceirh 23 27 infected infect VBN cord-258379-v3lceirh 23 28 cells cell NNS cord-258379-v3lceirh 23 29 was be VBD cord-258379-v3lceirh 23 30 layered layer VBN cord-258379-v3lceirh 23 31 onto onto IN cord-258379-v3lceirh 23 32 two two CD cord-258379-v3lceirh 23 33 tubes tube NNS cord-258379-v3lceirh 23 34 each each DT cord-258379-v3lceirh 23 35 containing contain VBG cord-258379-v3lceirh 23 36 10 10 CD cord-258379-v3lceirh 23 37 ml ml NNS cord-258379-v3lceirh 23 38 of of IN cord-258379-v3lceirh 23 39 20 20 CD cord-258379-v3lceirh 23 40 % % NN cord-258379-v3lceirh 23 41 ( ( -LRB- cord-258379-v3lceirh 23 42 w w NN cord-258379-v3lceirh 23 43 / / SYM cord-258379-v3lceirh 23 44 v v NN cord-258379-v3lceirh 23 45 ) ) -RRB- cord-258379-v3lceirh 23 46 TNE tne NN cord-258379-v3lceirh 23 47 - - HYPH cord-258379-v3lceirh 23 48 buffered buffer VBN cord-258379-v3lceirh 23 49 sucrose sucrose NN cord-258379-v3lceirh 23 50 solution solution NN cord-258379-v3lceirh 23 51 ( ( -LRB- cord-258379-v3lceirh 23 52 TNE TNE NNP cord-258379-v3lceirh 23 53 buffer buffer NN cord-258379-v3lceirh 23 54 : : : cord-258379-v3lceirh 23 55 50 50 CD cord-258379-v3lceirh 23 56 mM mM NNP cord-258379-v3lceirh 23 57 Tris Tris NNP cord-258379-v3lceirh 23 58 - - HYPH cord-258379-v3lceirh 23 59 HCI HCI NNP cord-258379-v3lceirh 23 60 , , , cord-258379-v3lceirh 23 61 pH pH NNP cord-258379-v3lceirh 23 62 7.4 7.4 CD cord-258379-v3lceirh 23 63 , , , cord-258379-v3lceirh 23 64 100 100 CD cord-258379-v3lceirh 23 65 mM mm CD cord-258379-v3lceirh 23 66 NaCI naci NN cord-258379-v3lceirh 23 67 , , , cord-258379-v3lceirh 23 68 1 1 CD cord-258379-v3lceirh 23 69 rnM rnm JJ cord-258379-v3lceirh 23 70 EDTA EDTA NNP cord-258379-v3lceirh 23 71 ) ) -RRB- cord-258379-v3lceirh 23 72 and and CC cord-258379-v3lceirh 23 73 centrifuged centrifuge VBD cord-258379-v3lceirh 23 74 at at IN cord-258379-v3lceirh 23 75 75,000 75,000 CD cord-258379-v3lceirh 23 76 g g NN cord-258379-v3lceirh 23 77 for for IN cord-258379-v3lceirh 23 78 3 3 CD cord-258379-v3lceirh 23 79 hr hr NN cord-258379-v3lceirh 23 80 at at IN cord-258379-v3lceirh 23 81 4 4 CD cord-258379-v3lceirh 23 82 ' ' '' cord-258379-v3lceirh 23 83 . . . cord-258379-v3lceirh 24 1 The the DT cord-258379-v3lceirh 24 2 resulting result VBG cord-258379-v3lceirh 24 3 virus virus NN cord-258379-v3lceirh 24 4 pellets pellet NNS cord-258379-v3lceirh 24 5 from from IN cord-258379-v3lceirh 24 6 the the DT cord-258379-v3lceirh 24 7 two two CD cord-258379-v3lceirh 24 8 tubes tube NNS cord-258379-v3lceirh 24 9 were be VBD cord-258379-v3lceirh 24 10 resuspended resuspend VBN cord-258379-v3lceirh 24 11 in in IN cord-258379-v3lceirh 24 12 10 10 CD cord-258379-v3lceirh 24 13 ml ml NNS cord-258379-v3lceirh 24 14 of of IN cord-258379-v3lceirh 24 15 TNE TNE NNP cord-258379-v3lceirh 24 16 buffer buffer NN cord-258379-v3lceirh 24 17 , , , cord-258379-v3lceirh 24 18 layered layer VBN cord-258379-v3lceirh 24 19 onto onto IN cord-258379-v3lceirh 24 20 a a DT cord-258379-v3lceirh 24 21 20-ml 20-ml CD cord-258379-v3lceirh 24 22 20 20 CD cord-258379-v3lceirh 24 23 - - SYM cord-258379-v3lceirh 24 24 55 55 CD cord-258379-v3lceirh 24 25 % % NN cord-258379-v3lceirh 24 26 linear linear JJ cord-258379-v3lceirh 24 27 sucrose sucrose NN cord-258379-v3lceirh 24 28 gradient gradient NN cord-258379-v3lceirh 24 29 prepared prepare VBN cord-258379-v3lceirh 24 30 with with IN cord-258379-v3lceirh 24 31 TNE TNE NNP cord-258379-v3lceirh 24 32 buffer buffer NN cord-258379-v3lceirh 24 33 , , , cord-258379-v3lceirh 24 34 and and CC cord-258379-v3lceirh 24 35 centrifuged centrifuge VBN cord-258379-v3lceirh 24 36 at at IN cord-258379-v3lceirh 24 37 75,000 75,000 CD cord-258379-v3lceirh 24 38 g g NN cord-258379-v3lceirh 24 39 for for IN cord-258379-v3lceirh 24 40 18 18 CD cord-258379-v3lceirh 24 41 hr hr NN cord-258379-v3lceirh 24 42 at at IN cord-258379-v3lceirh 24 43 4 4 CD cord-258379-v3lceirh 24 44 " " '' cord-258379-v3lceirh 24 45 . . . cord-258379-v3lceirh 25 1 The the DT cord-258379-v3lceirh 25 2 gradient gradient NN cord-258379-v3lceirh 25 3 was be VBD cord-258379-v3lceirh 25 4 fractionated fractionate VBN cord-258379-v3lceirh 25 5 ( ( -LRB- cord-258379-v3lceirh 25 6 22 22 CD cord-258379-v3lceirh 25 7 samples sample NNS cord-258379-v3lceirh 25 8 ) ) -RRB- cord-258379-v3lceirh 25 9 and and CC cord-258379-v3lceirh 25 10 the the DT cord-258379-v3lceirh 25 11 radioactivity radioactivity NN cord-258379-v3lceirh 25 12 present present JJ cord-258379-v3lceirh 25 13 in in IN cord-258379-v3lceirh 25 14 each each DT cord-258379-v3lceirh 25 15 fraction fraction NN cord-258379-v3lceirh 25 16 determined determine VBN cord-258379-v3lceirh 25 17 directly directly RB cord-258379-v3lceirh 25 18 by by IN cord-258379-v3lceirh 25 19 liquid liquid JJ cord-258379-v3lceirh 25 20 scintillation scintillation NN cord-258379-v3lceirh 25 21 . . . cord-258379-v3lceirh 26 1 Three three CD cord-258379-v3lceirh 26 2 adjacent adjacent JJ cord-258379-v3lceirh 26 3 fractions fraction NNS cord-258379-v3lceirh 26 4 which which WDT cord-258379-v3lceirh 26 5 contained contain VBD cord-258379-v3lceirh 26 6 most most JJS cord-258379-v3lceirh 26 7 ( ( -LRB- cord-258379-v3lceirh 26 8 70 70 CD cord-258379-v3lceirh 26 9 % % NN cord-258379-v3lceirh 26 10 ) ) -RRB- cord-258379-v3lceirh 26 11 of of IN cord-258379-v3lceirh 26 12 the the DT cord-258379-v3lceirh 26 13 radioactivity radioactivity NN cord-258379-v3lceirh 26 14 were be VBD cord-258379-v3lceirh 26 15 pooled pool VBN cord-258379-v3lceirh 26 16 , , , cord-258379-v3lceirh 26 17 diluted dilute VBN cord-258379-v3lceirh 26 18 three three CD cord-258379-v3lceirh 26 19 times time NNS cord-258379-v3lceirh 26 20 in in IN cord-258379-v3lceirh 26 21 TNE TNE NNP cord-258379-v3lceirh 26 22 buffer buffer NN cord-258379-v3lceirh 26 23 , , , cord-258379-v3lceirh 26 24 and and CC cord-258379-v3lceirh 26 25 applied apply VBD cord-258379-v3lceirh 26 26 to to IN cord-258379-v3lceirh 26 27 a a DT cord-258379-v3lceirh 26 28 second second JJ cord-258379-v3lceirh 26 29 density density NN cord-258379-v3lceirh 26 30 gradient gradient NN cord-258379-v3lceirh 26 31 consisting consist VBG cord-258379-v3lceirh 26 32 of of IN cord-258379-v3lceirh 26 33 20 20 CD cord-258379-v3lceirh 26 34 ml ml NNS cord-258379-v3lceirh 26 35 of of IN cord-258379-v3lceirh 26 36 1 1 CD cord-258379-v3lceirh 26 37 O-50 O-50 NNP cord-258379-v3lceirh 26 38 % % NN cord-258379-v3lceirh 26 39 Renografin Renografin NNP cord-258379-v3lceirh 26 40 ( ( -LRB- cord-258379-v3lceirh 26 41 meglumine meglumine NNP cord-258379-v3lceirh 26 42 diatrizoate diatrizoate NN cord-258379-v3lceirh 26 43 , , , cord-258379-v3lceirh 27 1 MW MW NNP cord-258379-v3lceirh 27 2 809.1 809.1 CD cord-258379-v3lceirh 27 3 , , , cord-258379-v3lceirh 27 4 Sigma Sigma NNP cord-258379-v3lceirh 27 5 ) ) -RRB- cord-258379-v3lceirh 27 6 prepared prepare VBD cord-258379-v3lceirh 27 7 with with IN cord-258379-v3lceirh 27 8 TNE TNE NNP cord-258379-v3lceirh 27 9 buffer buffer NN cord-258379-v3lceirh 27 10 . . . cord-258379-v3lceirh 28 1 This this DT cord-258379-v3lceirh 28 2 gradient gradient NN cord-258379-v3lceirh 28 3 was be VBD cord-258379-v3lceirh 28 4 centrifuged centrifuge VBN cord-258379-v3lceirh 28 5 and and CC cord-258379-v3lceirh 28 6 fractionated fractionate VBN cord-258379-v3lceirh 28 7 as as IN cord-258379-v3lceirh 28 8 before before RB cord-258379-v3lceirh 28 9 . . . cord-258379-v3lceirh 29 1 Aliquots Aliquots NNP cord-258379-v3lceirh 29 2 of of IN cord-258379-v3lceirh 29 3 13 13 CD cord-258379-v3lceirh 29 4 fractions fraction NNS cord-258379-v3lceirh 29 5 starting start VBG cord-258379-v3lceirh 29 6 from from IN cord-258379-v3lceirh 29 7 the the DT cord-258379-v3lceirh 29 8 bottom bottom NN cord-258379-v3lceirh 29 9 of of IN cord-258379-v3lceirh 29 10 the the DT cord-258379-v3lceirh 29 11 gradient gradient NN cord-258379-v3lceirh 29 12 were be VBD cord-258379-v3lceirh 29 13 analyzed analyze VBN cord-258379-v3lceirh 29 14 by by IN cord-258379-v3lceirh 29 15 SDS SDS NNP cord-258379-v3lceirh 29 16 - - HYPH cord-258379-v3lceirh 29 17 polyacrylamide polyacrylamide NN cord-258379-v3lceirh 29 18 gel gel NN cord-258379-v3lceirh 29 19 electrophoresis electrophoresis NN cord-258379-v3lceirh 29 20 ( ( -LRB- cord-258379-v3lceirh 29 21 PAGE page NN cord-258379-v3lceirh 29 22 ) ) -RRB- cord-258379-v3lceirh 29 23 ( ( -LRB- cord-258379-v3lceirh 29 24 Fig Fig NNP cord-258379-v3lceirh 29 25 . . . cord-258379-v3lceirh 29 26 1 1 CD cord-258379-v3lceirh 29 27 a a LS cord-258379-v3lceirh 29 28 ) ) -RRB- cord-258379-v3lceirh 29 29 . . . cord-258379-v3lceirh 30 1 Prominent prominent JJ cord-258379-v3lceirh 30 2 radiolabeled radiolabeled JJ cord-258379-v3lceirh 30 3 bands band NNS cord-258379-v3lceirh 30 4 corresponding correspond VBG cord-258379-v3lceirh 30 5 to to IN cord-258379-v3lceirh 30 6 the the DT cord-258379-v3lceirh 30 7 known know VBN cord-258379-v3lceirh 30 8 structural structural JJ cord-258379-v3lceirh 30 9 proteins protein NNS cord-258379-v3lceirh 30 10 of of IN cord-258379-v3lceirh 30 11 IBV IBV NNP cord-258379-v3lceirh 30 12 ( ( -LRB- cord-258379-v3lceirh 30 13 6 6 CD cord-258379-v3lceirh 30 14 , , , cord-258379-v3lceirh 30 15 24 24 CD cord-258379-v3lceirh 30 16 ) ) -RRB- cord-258379-v3lceirh 30 17 were be VBD cord-258379-v3lceirh 30 18 observed observe VBN cord-258379-v3lceirh 30 19 in in IN cord-258379-v3lceirh 30 20 fractions fraction NNS cord-258379-v3lceirh 30 21 7-l 7-l CD cord-258379-v3lceirh 30 22 0 0 NFP cord-258379-v3lceirh 30 23 ( ( -LRB- cord-258379-v3lceirh 30 24 lanes lane NNS cord-258379-v3lceirh 30 25 1 1 CD cord-258379-v3lceirh 30 26 O o NN cord-258379-v3lceirh 30 27 - - HYPH cord-258379-v3lceirh 30 28 l l NN cord-258379-v3lceirh 30 29 4 4 CD cord-258379-v3lceirh 30 30 ) ) -RRB- cord-258379-v3lceirh 30 31 . . . cord-258379-v3lceirh 31 1 The the DT cord-258379-v3lceirh 31 2 sedimentation sedimentation NN cord-258379-v3lceirh 31 3 rate rate NN cord-258379-v3lceirh 31 4 of of IN cord-258379-v3lceirh 31 5 these these DT cord-258379-v3lceirh 31 6 proteins protein NNS cord-258379-v3lceirh 31 7 corresponded correspond VBD cord-258379-v3lceirh 31 8 with with IN cord-258379-v3lceirh 31 9 that that DT cord-258379-v3lceirh 31 10 reported report VBN cord-258379-v3lceirh 31 11 originally originally RB cord-258379-v3lceirh 31 12 for for IN cord-258379-v3lceirh 31 13 IBV IBV NNP cord-258379-v3lceirh 31 14 virions virion NNS cord-258379-v3lceirh 31 15 by by IN cord-258379-v3lceirh 31 16 Stern Stern NNP cord-258379-v3lceirh 31 17 et et FW cord-258379-v3lceirh 31 18 al al NNP cord-258379-v3lceirh 31 19 . . . cord-258379-v3lceirh 32 1 ( ( -LRB- cord-258379-v3lceirh 32 2 24 24 CD cord-258379-v3lceirh 32 3 ) ) -RRB- cord-258379-v3lceirh 32 4 whose whose WP$ cord-258379-v3lceirh 32 5 basic basic JJ cord-258379-v3lceirh 32 6 protocol protocol NN cord-258379-v3lceirh 32 7 was be VBD cord-258379-v3lceirh 32 8 used use VBN cord-258379-v3lceirh 32 9 for for IN cord-258379-v3lceirh 32 10 the the DT cord-258379-v3lceirh 32 11 purification purification NN cord-258379-v3lceirh 32 12 carried carry VBN cord-258379-v3lceirh 32 13 out out RP cord-258379-v3lceirh 32 14 here here RB cord-258379-v3lceirh 32 15 . . . cord-258379-v3lceirh 33 1 We -PRON- PRP cord-258379-v3lceirh 33 2 also also RB cord-258379-v3lceirh 33 3 observed observe VBD cord-258379-v3lceirh 33 4 that that IN cord-258379-v3lceirh 33 5 a a DT cord-258379-v3lceirh 33 6 polypeptide polypeptide NN cord-258379-v3lceirh 33 7 of of IN cord-258379-v3lceirh 33 8 approximately approximately RB cord-258379-v3lceirh 33 9 12 12 CD cord-258379-v3lceirh 33 10 K K NNP cord-258379-v3lceirh 33 11 , , , cord-258379-v3lceirh 33 12 with with IN cord-258379-v3lceirh 33 13 an an DT cord-258379-v3lceirh 33 14 electrophoretic electrophoretic JJ cord-258379-v3lceirh 33 15 mobility mobility NN cord-258379-v3lceirh 33 16 similar similar JJ cord-258379-v3lceirh 33 17 to to IN cord-258379-v3lceirh 33 18 that that DT cord-258379-v3lceirh 33 19 of of IN cord-258379-v3lceirh 33 20 in in IN cord-258379-v3lceirh 33 21 vitrotranslated vitrotranslate VBN cord-258379-v3lceirh 33 22 3c 3c NNP cord-258379-v3lceirh 33 23 ( ( -LRB- cord-258379-v3lceirh 33 24 23 23 CD cord-258379-v3lceirh 33 25 ) ) -RRB- cord-258379-v3lceirh 33 26 cofractionated cofractionate VBD cord-258379-v3lceirh 33 27 exactly exactly RB cord-258379-v3lceirh 33 28 on on IN cord-258379-v3lceirh 33 29 the the DT cord-258379-v3lceirh 33 30 gradient gradient NN cord-258379-v3lceirh 33 31 with with IN cord-258379-v3lceirh 33 32 the the DT cord-258379-v3lceirh 33 33 virus virus NN cord-258379-v3lceirh 33 34 major major JJ cord-258379-v3lceirh 33 35 structural structural JJ cord-258379-v3lceirh 33 36 proteins protein NNS cord-258379-v3lceirh 33 37 , , , cord-258379-v3lceirh 33 38 suggesting suggest VBG cord-258379-v3lceirh 33 39 that that IN cord-258379-v3lceirh 33 40 it -PRON- PRP cord-258379-v3lceirh 33 41 was be VBD cord-258379-v3lceirh 33 42 associated associate VBN cord-258379-v3lceirh 33 43 with with IN cord-258379-v3lceirh 33 44 virus virus NN cord-258379-v3lceirh 33 45 particles particle NNS cord-258379-v3lceirh 33 46 . . . cord-258379-v3lceirh 34 1 Polypeptides polypeptide NNS cord-258379-v3lceirh 34 2 corresponding correspond VBG cord-258379-v3lceirh 34 3 to to IN cord-258379-v3lceirh 34 4 in in FW cord-258379-v3lceirh 34 5 vitro vitro FW cord-258379-v3lceirh 34 6 - - HYPH cord-258379-v3lceirh 34 7 translated translate VBN cord-258379-v3lceirh 34 8 5a 5a CD cord-258379-v3lceirh 34 9 , , , cord-258379-v3lceirh 34 10 5b 5b NN cord-258379-v3lceirh 34 11 , , , cord-258379-v3lceirh 34 12 3a 3a NNP cord-258379-v3lceirh 34 13 , , , cord-258379-v3lceirh 34 14 and and CC cord-258379-v3lceirh 34 15 3b 3b NNP cord-258379-v3lceirh 34 16 ( ( -LRB- cord-258379-v3lceirh 34 17 17 17 CD cord-258379-v3lceirh 34 18 , , , cord-258379-v3lceirh 34 19 18 18 CD cord-258379-v3lceirh 34 20 ) ) -RRB- cord-258379-v3lceirh 34 21 however however RB cord-258379-v3lceirh 34 22 , , , cord-258379-v3lceirh 34 23 were be VBD cord-258379-v3lceirh 34 24 not not RB cord-258379-v3lceirh 34 25 detectable detectable JJ cord-258379-v3lceirh 34 26 . . . cord-258379-v3lceirh 35 1 A a DT cord-258379-v3lceirh 35 2 radiolabeled radiolabele VBN cord-258379-v3lceirh 35 3 protein protein NN cord-258379-v3lceirh 35 4 with with IN cord-258379-v3lceirh 35 5 an an DT cord-258379-v3lceirh 35 6 apparent apparent JJ cord-258379-v3lceirh 35 7 molecular molecular JJ cord-258379-v3lceirh 35 8 weight weight NN cord-258379-v3lceirh 35 9 of of IN cord-258379-v3lceirh 35 10 44 44 CD cord-258379-v3lceirh 35 11 K K NNP cord-258379-v3lceirh 35 12 , , , cord-258379-v3lceirh 35 13 appearing appear VBG cord-258379-v3lceirh 35 14 in in IN cord-258379-v3lceirh 35 15 fractions fraction NNS cord-258379-v3lceirh 35 16 l-6 l-6 NN cord-258379-v3lceirh 35 17 ( ( -LRB- cord-258379-v3lceirh 35 18 lanes lane NNS cord-258379-v3lceirh 35 19 4 4 CD cord-258379-v3lceirh 35 20 - - SYM cord-258379-v3lceirh 35 21 9 9 CD cord-258379-v3lceirh 35 22 ) ) -RRB- cord-258379-v3lceirh 35 23 , , , cord-258379-v3lceirh 35 24 probably probably RB cord-258379-v3lceirh 35 25 represents represent VBZ cord-258379-v3lceirh 35 26 cellular cellular JJ cord-258379-v3lceirh 35 27 actin actin NN cord-258379-v3lceirh 35 28 as as IN cord-258379-v3lceirh 35 29 previously previously RB cord-258379-v3lceirh 35 30 reported report VBN cord-258379-v3lceirh 35 31 ( ( -LRB- cord-258379-v3lceirh 35 32 19 19 CD cord-258379-v3lceirh 35 33 ) ) -RRB- cord-258379-v3lceirh 35 34 . . . cord-258379-v3lceirh 36 1 To to TO cord-258379-v3lceirh 36 2 confirm confirm VB cord-258379-v3lceirh 36 3 further further RB cord-258379-v3lceirh 36 4 the the DT cord-258379-v3lceirh 36 5 association association NN cord-258379-v3lceirh 36 6 of of IN cord-258379-v3lceirh 36 7 the the DT cord-258379-v3lceirh 36 8 putative putative JJ cord-258379-v3lceirh 36 9 12 12 CD cord-258379-v3lceirh 36 10 K K NNP cord-258379-v3lceirh 36 11 polypeptide polypeptide NN cord-258379-v3lceirh 36 12 with with IN cord-258379-v3lceirh 36 13 virions virion NNS cord-258379-v3lceirh 36 14 , , , cord-258379-v3lceirh 36 15 fractions fraction NNS cord-258379-v3lceirh 36 16 7 7 CD cord-258379-v3lceirh 36 17 , , , cord-258379-v3lceirh 36 18 8 8 CD cord-258379-v3lceirh 36 19 , , , cord-258379-v3lceirh 36 20 and and CC cord-258379-v3lceirh 36 21 9 9 CD cord-258379-v3lceirh 36 22 ( ( -LRB- cord-258379-v3lceirh 36 23 lanes lane NNS cord-258379-v3lceirh 36 24 10 10 CD cord-258379-v3lceirh 36 25 , , , cord-258379-v3lceirh 36 26 11 11 CD cord-258379-v3lceirh 36 27 , , , cord-258379-v3lceirh 36 28 and and CC cord-258379-v3lceirh 36 29 12 12 CD cord-258379-v3lceirh 36 30 ) ) -RRB- cord-258379-v3lceirh 36 31 which which WDT cord-258379-v3lceirh 36 32 contained contain VBD cord-258379-v3lceirh 36 33 most most JJS cord-258379-v3lceirh 36 34 of of IN cord-258379-v3lceirh 36 35 the the DT cord-258379-v3lceirh 36 36 radioactive radioactive JJ cord-258379-v3lceirh 36 37 virions virion NNS cord-258379-v3lceirh 36 38 , , , cord-258379-v3lceirh 36 39 were be VBD cord-258379-v3lceirh 36 40 diluted dilute VBN cord-258379-v3lceirh 36 41 threefold threefold RB cord-258379-v3lceirh 36 42 with with IN cord-258379-v3lceirh 36 43 TNE TNE NNP cord-258379-v3lceirh 36 44 buffer buffer NN cord-258379-v3lceirh 36 45 , , , cord-258379-v3lceirh 36 46 pooled pool VBN cord-258379-v3lceirh 36 47 , , , cord-258379-v3lceirh 36 48 and and CC cord-258379-v3lceirh 36 49 fractionated fractionate VBN cord-258379-v3lceirh 36 50 on on IN cord-258379-v3lceirh 36 51 a a DT cord-258379-v3lceirh 36 52 second second JJ cord-258379-v3lceirh 36 53 20 20 CD cord-258379-v3lceirh 36 54 - - SYM cord-258379-v3lceirh 36 55 55 55 CD cord-258379-v3lceirh 36 56 % % NN cord-258379-v3lceirh 36 57 linear linear JJ cord-258379-v3lceirh 36 58 sucrose sucrose NN cord-258379-v3lceirh 36 59 gradient gradient NN cord-258379-v3lceirh 36 60 under under IN cord-258379-v3lceirh 36 61 the the DT cord-258379-v3lceirh 36 62 same same JJ cord-258379-v3lceirh 36 63 conditions condition NNS cord-258379-v3lceirh 36 64 as as IN cord-258379-v3lceirh 36 65 before before RB cord-258379-v3lceirh 36 66 . . . cord-258379-v3lceirh 37 1 Twenty twenty CD cord-258379-v3lceirh 37 2 - - HYPH cord-258379-v3lceirh 37 3 two two CD cord-258379-v3lceirh 37 4 fractions fraction NNS cord-258379-v3lceirh 37 5 were be VBD cord-258379-v3lceirh 37 6 collected collect VBN cord-258379-v3lceirh 37 7 , , , cord-258379-v3lceirh 37 8 of of IN cord-258379-v3lceirh 37 9 which which WDT cord-258379-v3lceirh 37 10 16 16 CD cord-258379-v3lceirh 37 11 ( ( -LRB- cord-258379-v3lceirh 37 12 starting start VBG cord-258379-v3lceirh 37 13 from from IN cord-258379-v3lceirh 37 14 the the DT cord-258379-v3lceirh 37 15 bottom bottom NN cord-258379-v3lceirh 37 16 of of IN cord-258379-v3lceirh 37 17 the the DT cord-258379-v3lceirh 37 18 gradient gradient NN cord-258379-v3lceirh 37 19 ) ) -RRB- cord-258379-v3lceirh 37 20 were be VBD cord-258379-v3lceirh 37 21 analyzed analyze VBN cord-258379-v3lceirh 37 22 by by IN cord-258379-v3lceirh 37 23 SDS SDS NNP cord-258379-v3lceirh 37 24 - - HYPH cord-258379-v3lceirh 37 25 PAGE PAGE NNP cord-258379-v3lceirh 37 26 ( ( -LRB- cord-258379-v3lceirh 37 27 Fig Fig NNP cord-258379-v3lceirh 37 28 . . . cord-258379-v3lceirh 37 29 1 1 CD cord-258379-v3lceirh 37 30 b b NN cord-258379-v3lceirh 37 31 ) ) -RRB- cord-258379-v3lceirh 37 32 . . . cord-258379-v3lceirh 38 1 Once once RB cord-258379-v3lceirh 38 2 again again RB cord-258379-v3lceirh 38 3 the the DT cord-258379-v3lceirh 38 4 12 12 CD cord-258379-v3lceirh 38 5 K K NNP cord-258379-v3lceirh 38 6 polypeptide polypeptide NN cord-258379-v3lceirh 38 7 was be VBD cord-258379-v3lceirh 38 8 clearly clearly RB cord-258379-v3lceirh 38 9 present present JJ cord-258379-v3lceirh 38 10 and and CC cord-258379-v3lceirh 38 11 cofractionated cofractionate VBD cord-258379-v3lceirh 38 12 exactly exactly RB cord-258379-v3lceirh 38 13 with with IN cord-258379-v3lceirh 38 14 the the DT cord-258379-v3lceirh 38 15 major major JJ cord-258379-v3lceirh 38 16 virion virion NN cord-258379-v3lceirh 38 17 structural structural JJ cord-258379-v3lceirh 38 18 proteins protein NNS cord-258379-v3lceirh 38 19 , , , cord-258379-v3lceirh 38 20 appearing appear VBG cord-258379-v3lceirh 38 21 mostly mostly RB cord-258379-v3lceirh 38 22 in in IN cord-258379-v3lceirh 38 23 fractions fraction NNS cord-258379-v3lceirh 38 24 5 5 CD cord-258379-v3lceirh 38 25 - - SYM cord-258379-v3lceirh 38 26 9 9 CD cord-258379-v3lceirh 38 27 ( ( -LRB- cord-258379-v3lceirh 38 28 lanes lane NNS cord-258379-v3lceirh 38 29 6-l 6-l CD cord-258379-v3lceirh 38 30 0 0 NFP cord-258379-v3lceirh 38 31 ) ) -RRB- cord-258379-v3lceirh 38 32 . . . cord-258379-v3lceirh 39 1 There there EX cord-258379-v3lceirh 39 2 was be VBD cord-258379-v3lceirh 39 3 considerably considerably RB cord-258379-v3lceirh 39 4 less less JJR cord-258379-v3lceirh 39 5 evidence evidence NN cord-258379-v3lceirh 39 6 of of IN cord-258379-v3lceirh 39 7 contaminating contaminate VBG cord-258379-v3lceirh 39 8 cellular cellular JJ cord-258379-v3lceirh 39 9 proteins protein NNS cord-258379-v3lceirh 39 10 in in IN cord-258379-v3lceirh 39 11 this this DT cord-258379-v3lceirh 39 12 gradient gradient NN cord-258379-v3lceirh 39 13 , , , cord-258379-v3lceirh 39 14 and and CC cord-258379-v3lceirh 39 15 the the DT cord-258379-v3lceirh 39 16 relative relative JJ cord-258379-v3lceirh 39 17 proportion proportion NN cord-258379-v3lceirh 39 18 of of IN cord-258379-v3lceirh 39 19 the the DT cord-258379-v3lceirh 39 20 12 12 CD cord-258379-v3lceirh 39 21 K K NNP cord-258379-v3lceirh 39 22 protein protein NN cord-258379-v3lceirh 39 23 to to IN cord-258379-v3lceirh 39 24 the the DT cord-258379-v3lceirh 39 25 other other JJ cord-258379-v3lceirh 39 26 virion virion NN cord-258379-v3lceirh 39 27 proteins protein NNS cord-258379-v3lceirh 39 28 appeared appear VBD cord-258379-v3lceirh 39 29 similar similar JJ cord-258379-v3lceirh 39 30 to to IN cord-258379-v3lceirh 39 31 that that DT cord-258379-v3lceirh 39 32 observed observe VBD cord-258379-v3lceirh 39 33 at at IN cord-258379-v3lceirh 39 34 the the DT cord-258379-v3lceirh 39 35 previous previous JJ cord-258379-v3lceirh 39 36 stage stage NN cord-258379-v3lceirh 39 37 in in IN cord-258379-v3lceirh 39 38 purification purification NN cord-258379-v3lceirh 39 39 , , , cord-258379-v3lceirh 39 40 strongly strongly RB cord-258379-v3lceirh 39 41 supporting support VBG cord-258379-v3lceirh 39 42 the the DT cord-258379-v3lceirh 39 43 idea idea NN cord-258379-v3lceirh 39 44 of of IN cord-258379-v3lceirh 39 45 a a DT cord-258379-v3lceirh 39 46 specific specific JJ cord-258379-v3lceirh 39 47 association association NN cord-258379-v3lceirh 39 48 between between IN cord-258379-v3lceirh 39 49 the the DT cord-258379-v3lceirh 39 50 12 12 CD cord-258379-v3lceirh 39 51 K K NNP cord-258379-v3lceirh 39 52 protein protein NN cord-258379-v3lceirh 39 53 and and CC cord-258379-v3lceirh 39 54 virus virus NN cord-258379-v3lceirh 39 55 particles particle NNS cord-258379-v3lceirh 39 56 . . . cord-258379-v3lceirh 40 1 A a DT cord-258379-v3lceirh 40 2 single single JJ cord-258379-v3lceirh 40 3 band band NN cord-258379-v3lceirh 40 4 migrating migrate VBG cord-258379-v3lceirh 40 5 at at IN cord-258379-v3lceirh 40 6 the the DT cord-258379-v3lceirh 40 7 position position NN cord-258379-v3lceirh 40 8 expected expect VBN cord-258379-v3lceirh 40 9 of of IN cord-258379-v3lceirh 40 10 one one CD cord-258379-v3lceirh 40 11 of of IN cord-258379-v3lceirh 40 12 the the DT cord-258379-v3lceirh 40 13 cleaved cleaved JJ cord-258379-v3lceirh 40 14 portions portion NNS cord-258379-v3lceirh 40 15 of of IN cord-258379-v3lceirh 40 16 the the DT cord-258379-v3lceirh 40 17 spike spike NN cord-258379-v3lceirh 40 18 protein protein NN cord-258379-v3lceirh 40 19 , , , cord-258379-v3lceirh 40 20 gp90 gp90 NNP cord-258379-v3lceirh 40 21 , , , cord-258379-v3lceirh 40 22 was be VBD cord-258379-v3lceirh 40 23 observed observe VBN cord-258379-v3lceirh 40 24 in in IN cord-258379-v3lceirh 40 25 the the DT cord-258379-v3lceirh 40 26 upper upper JJ cord-258379-v3lceirh 40 27 part part NN cord-258379-v3lceirh 40 28 of of IN cord-258379-v3lceirh 40 29 the the DT cord-258379-v3lceirh 40 30 gradient gradient NN cord-258379-v3lceirh 40 31 , , , cord-258379-v3lceirh 40 32 which which WDT cord-258379-v3lceirh 40 33 may may MD cord-258379-v3lceirh 40 34 represent represent VB cord-258379-v3lceirh 40 35 the the DT cord-258379-v3lceirh 40 36 degraded degraded JJ cord-258379-v3lceirh 40 37 product product NN cord-258379-v3lceirh 40 38 of of IN cord-258379-v3lceirh 40 39 the the DT cord-258379-v3lceirh 40 40 spikes spike NNS cord-258379-v3lceirh 40 41 ; ; : cord-258379-v3lceirh 40 42 morphological morphological JJ cord-258379-v3lceirh 40 43 examination examination NN cord-258379-v3lceirh 40 44 of of IN cord-258379-v3lceirh 40 45 unlabeled unlabeled JJ cord-258379-v3lceirh 40 46 equivalent equivalent JJ cord-258379-v3lceirh 40 47 preparations preparation NNS cord-258379-v3lceirh 40 48 at at IN cord-258379-v3lceirh 40 49 this this DT cord-258379-v3lceirh 40 50 stage stage NN cord-258379-v3lceirh 40 51 by by IN cord-258379-v3lceirh 40 52 electron electron NN cord-258379-v3lceirh 40 53 microscopy microscopy NNS cord-258379-v3lceirh 40 54 after after IN cord-258379-v3lceirh 40 55 negative negative JJ cord-258379-v3lceirh 40 56 staining staining NN cord-258379-v3lceirh 40 57 showed show VBD cord-258379-v3lceirh 40 58 that that IN cord-258379-v3lceirh 40 59 a a DT cord-258379-v3lceirh 40 60 quarter quarter NN cord-258379-v3lceirh 40 61 of of IN cord-258379-v3lceirh 40 62 the the DT cord-258379-v3lceirh 40 63 virions virion NNS cord-258379-v3lceirh 40 64 had have VBD cord-258379-v3lceirh 40 65 lost lose VBN cord-258379-v3lceirh 40 66 their -PRON- PRP$ cord-258379-v3lceirh 40 67 spike spike NN cord-258379-v3lceirh 40 68 structures structure NNS cord-258379-v3lceirh 40 69 ( ( -LRB- cord-258379-v3lceirh 40 70 data datum NNS cord-258379-v3lceirh 40 71 not not RB cord-258379-v3lceirh 40 72 shown show VBN cord-258379-v3lceirh 40 73 ) ) -RRB- cord-258379-v3lceirh 40 74 . . . cord-258379-v3lceirh 41 1 In in IN cord-258379-v3lceirh 41 2 order order NN cord-258379-v3lceirh 41 3 to to TO cord-258379-v3lceirh 41 4 establish establish VB cord-258379-v3lceirh 41 5 the the DT cord-258379-v3lceirh 41 6 identity identity NN cord-258379-v3lceirh 41 7 of of IN cord-258379-v3lceirh 41 8 the the DT cord-258379-v3lceirh 41 9 12 12 CD cord-258379-v3lceirh 41 10 K K NNP cord-258379-v3lceirh 41 11 polypeptide polypeptide NN cord-258379-v3lceirh 41 12 as as IN cord-258379-v3lceirh 41 13 the the DT cord-258379-v3lceirh 41 14 product product NN cord-258379-v3lceirh 41 15 of of IN cord-258379-v3lceirh 41 16 the the DT cord-258379-v3lceirh 41 17 3c 3c NNP cord-258379-v3lceirh 41 18 gene gene NN cord-258379-v3lceirh 41 19 , , , cord-258379-v3lceirh 41 20 and and CC cord-258379-v3lceirh 41 21 to to TO cord-258379-v3lceirh 41 22 examine examine VB cord-258379-v3lceirh 41 23 the the DT cord-258379-v3lceirh 41 24 possibility possibility NN cord-258379-v3lceirh 41 25 that that IN cord-258379-v3lceirh 41 26 IBV IBV NNP cord-258379-v3lceirh 41 27 virions virion NNS cord-258379-v3lceirh 41 28 might may MD cord-258379-v3lceirh 41 29 contain contain VB cord-258379-v3lceirh 41 30 other other JJ cord-258379-v3lceirh 41 31 new new JJ cord-258379-v3lceirh 41 32 virus virus NN cord-258379-v3lceirh 41 33 polypeptides polypeptide NNS cord-258379-v3lceirh 41 34 in in IN cord-258379-v3lceirh 41 35 amounts amount NNS cord-258379-v3lceirh 41 36 too too RB cord-258379-v3lceirh 41 37 small small JJ cord-258379-v3lceirh 41 38 to to TO cord-258379-v3lceirh 41 39 detect detect VB cord-258379-v3lceirh 41 40 directly directly RB cord-258379-v3lceirh 41 41 , , , cord-258379-v3lceirh 41 42 we -PRON- PRP cord-258379-v3lceirh 41 43 next next RB cord-258379-v3lceirh 41 44 carried carry VBD cord-258379-v3lceirh 41 45 out out RP cord-258379-v3lceirh 41 46 immunoprecipitation immunoprecipitation NN cord-258379-v3lceirh 41 47 experiments experiment NNS cord-258379-v3lceirh 41 48 using use VBG cord-258379-v3lceirh 41 49 monospecific monospecific JJ cord-258379-v3lceirh 41 50 antisera antisera NN cord-258379-v3lceirh 41 51 directed direct VBN cord-258379-v3lceirh 41 52 against against IN cord-258379-v3lceirh 41 53 the the DT cord-258379-v3lceirh 41 54 predicted predict VBN cord-258379-v3lceirh 41 55 products product NNS cord-258379-v3lceirh 41 56 of of IN cord-258379-v3lceirh 41 57 the the DT cord-258379-v3lceirh 41 58 3a 3a NNP cord-258379-v3lceirh 41 59 , , , cord-258379-v3lceirh 41 60 3b 3b NNP cord-258379-v3lceirh 41 61 , , , cord-258379-v3lceirh 41 62 3c 3c NNP cord-258379-v3lceirh 41 63 , , , cord-258379-v3lceirh 41 64 5a 5a CD cord-258379-v3lceirh 41 65 , , , cord-258379-v3lceirh 41 66 and and CC cord-258379-v3lceirh 41 67 5b 5b NN cord-258379-v3lceirh 41 68 ORFs ORFs NNP cord-258379-v3lceirh 41 69 ( ( -LRB- cord-258379-v3lceirh 41 70 17 17 CD cord-258379-v3lceirh 41 71 , , , cord-258379-v3lceirh 41 72 18 18 CD cord-258379-v3lceirh 41 73 , , , cord-258379-v3lceirh 41 74 23 23 CD cord-258379-v3lceirh 41 75 ) ) -RRB- cord-258379-v3lceirh 41 76 . . . cord-258379-v3lceirh 42 1 For for IN cord-258379-v3lceirh 42 2 this this DT cord-258379-v3lceirh 42 3 purpose purpose NN cord-258379-v3lceirh 42 4 , , , cord-258379-v3lceirh 42 5 fractions fraction NNS cord-258379-v3lceirh 42 6 5 5 CD cord-258379-v3lceirh 42 7 , , , cord-258379-v3lceirh 42 8 6 6 CD cord-258379-v3lceirh 42 9 , , , cord-258379-v3lceirh 42 10 and and CC cord-258379-v3lceirh 42 11 7 7 CD cord-258379-v3lceirh 42 12 from from IN cord-258379-v3lceirh 42 13 the the DT cord-258379-v3lceirh 42 14 second second JJ cord-258379-v3lceirh 42 15 sucrose sucrose NN cord-258379-v3lceirh 42 16 gradient gradient NN cord-258379-v3lceirh 42 17 ( ( -LRB- cord-258379-v3lceirh 42 18 lanes lane NNS cord-258379-v3lceirh 42 19 6 6 CD cord-258379-v3lceirh 42 20 , , , cord-258379-v3lceirh 42 21 7 7 CD cord-258379-v3lceirh 42 22 , , , cord-258379-v3lceirh 42 23 and and CC cord-258379-v3lceirh 42 24 8 8 CD cord-258379-v3lceirh 42 25 , , , cord-258379-v3lceirh 42 26 Fig Fig NNP cord-258379-v3lceirh 42 27 . . NNP cord-258379-v3lceirh 42 28 1 1 CD cord-258379-v3lceirh 42 29 b b NN cord-258379-v3lceirh 42 30 ) ) -RRB- cord-258379-v3lceirh 42 31 were be VBD cord-258379-v3lceirh 42 32 pooled pool VBN cord-258379-v3lceirh 42 33 , , , cord-258379-v3lceirh 42 34 diluted dilute VBN cord-258379-v3lceirh 42 35 three three CD cord-258379-v3lceirh 42 36 times time NNS cord-258379-v3lceirh 42 37 in in IN cord-258379-v3lceirh 42 38 TNE TNE NNP cord-258379-v3lceirh 42 39 buffer buffer NN cord-258379-v3lceirh 42 40 , , , cord-258379-v3lceirh 42 41 and and CC cord-258379-v3lceirh 42 42 centrifuged centrifuge VBN cord-258379-v3lceirh 42 43 at at IN cord-258379-v3lceirh 42 44 65,000 65,000 CD cord-258379-v3lceirh 42 45 g g NN cord-258379-v3lceirh 42 46 for for IN cord-258379-v3lceirh 42 47 3 3 CD cord-258379-v3lceirh 42 48 hr hr NN cord-258379-v3lceirh 42 49 at at IN cord-258379-v3lceirh 42 50 4 4 CD cord-258379-v3lceirh 42 51 ' ' '' cord-258379-v3lceirh 42 52 to to TO cord-258379-v3lceirh 42 53 pellet pellet VB cord-258379-v3lceirh 42 54 the the DT cord-258379-v3lceirh 42 55 virus virus NN cord-258379-v3lceirh 42 56 . . . cord-258379-v3lceirh 43 1 The the DT cord-258379-v3lceirh 43 2 virus virus NN cord-258379-v3lceirh 43 3 pellet pellet NN cord-258379-v3lceirh 43 4 was be VBD cord-258379-v3lceirh 43 5 then then RB cord-258379-v3lceirh 43 6 resuspended resuspend VBN cord-258379-v3lceirh 43 7 in in IN cord-258379-v3lceirh 43 8 RIPA RIPA NNP cord-258379-v3lceirh 43 9 buffer buffer NN cord-258379-v3lceirh 43 10 ( ( -LRB- cord-258379-v3lceirh 43 11 50 50 CD cord-258379-v3lceirh 43 12 mll mll NN cord-258379-v3lceirh 43 13 / / SYM cord-258379-v3lceirh 43 14 l l NNP cord-258379-v3lceirh 43 15 Tris Tris NNP cord-258379-v3lceirh 43 16 - - HYPH cord-258379-v3lceirh 43 17 HCI HCI NNP cord-258379-v3lceirh 43 18 , , , cord-258379-v3lceirh 43 19 pH pH NNP cord-258379-v3lceirh 43 20 7.5 7.5 CD cord-258379-v3lceirh 43 21 , , , cord-258379-v3lceirh 43 22 150 150 CD cord-258379-v3lceirh 43 23 mM mm CD cord-258379-v3lceirh 43 24 NaCI naci NN cord-258379-v3lceirh 43 25 , , , cord-258379-v3lceirh 43 26 1 1 CD cord-258379-v3lceirh 43 27 % % NN cord-258379-v3lceirh 43 28 NP-40 NP-40 NNP cord-258379-v3lceirh 43 29 , , , cord-258379-v3lceirh 43 30 0.1 0.1 CD cord-258379-v3lceirh 43 31 % % NN cord-258379-v3lceirh 43 32 SDS SDS NNP cord-258379-v3lceirh 43 33 , , , cord-258379-v3lceirh 43 34 10 10 CD cord-258379-v3lceirh 43 35 % % NN cord-258379-v3lceirh 43 36 sodium sodium NN cord-258379-v3lceirh 43 37 deoxycholate deoxycholate NN cord-258379-v3lceirh 43 38 ) ) -RRB- cord-258379-v3lceirh 43 39 and and CC cord-258379-v3lceirh 43 40 aliquots aliquot NNS cord-258379-v3lceirh 43 41 were be VBD cord-258379-v3lceirh 43 42 immunoprecipitated immunoprecipitate VBN cord-258379-v3lceirh 43 43 with with IN cord-258379-v3lceirh 43 44 a a DT cord-258379-v3lceirh 43 45 series series NN cord-258379-v3lceirh 43 46 of of IN cord-258379-v3lceirh 43 47 specific specific JJ cord-258379-v3lceirh 43 48 and and CC cord-258379-v3lceirh 43 49 control control NN cord-258379-v3lceirh 43 50 antisera antisera NNP cord-258379-v3lceirh 43 51 as as IN cord-258379-v3lceirh 43 52 described describe VBN cord-258379-v3lceirh 43 53 ( ( -LRB- cord-258379-v3lceirh 43 54 5 5 CD cord-258379-v3lceirh 43 55 ) ) -RRB- cord-258379-v3lceirh 43 56 . . . cord-258379-v3lceirh 44 1 The the DT cord-258379-v3lceirh 44 2 result result NN cord-258379-v3lceirh 44 3 of of IN cord-258379-v3lceirh 44 4 this this DT cord-258379-v3lceirh 44 5 experiment experiment NN cord-258379-v3lceirh 44 6 ( ( -LRB- cord-258379-v3lceirh 44 7 Fig Fig NNP cord-258379-v3lceirh 44 8 . . . cord-258379-v3lceirh 44 9 2a 2a LS cord-258379-v3lceirh 44 10 ) ) -RRB- cord-258379-v3lceirh 44 11 showed show VBD cord-258379-v3lceirh 44 12 clearly clearly RB cord-258379-v3lceirh 44 13 that that IN cord-258379-v3lceirh 44 14 the the DT cord-258379-v3lceirh 44 15 12 12 CD cord-258379-v3lceirh 44 16 K K NNP cord-258379-v3lceirh 44 17 polypeptide polypeptide NN cord-258379-v3lceirh 44 18 was be VBD cord-258379-v3lceirh 44 19 immunoprecipitated immunoprecipitate VBN cord-258379-v3lceirh 44 20 by by IN cord-258379-v3lceirh 44 21 anti-3c anti-3c NNP cord-258379-v3lceirh 44 22 antiserum antiserum NN cord-258379-v3lceirh 44 23 , , , cord-258379-v3lceirh 44 24 but but CC cord-258379-v3lceirh 44 25 not not RB cord-258379-v3lceirh 44 26 by by IN cord-258379-v3lceirh 44 27 any any DT cord-258379-v3lceirh 44 28 of of IN cord-258379-v3lceirh 44 29 the the DT cord-258379-v3lceirh 44 30 other other JJ cord-258379-v3lceirh 44 31 antisera antisera NN cord-258379-v3lceirh 44 32 . . . cord-258379-v3lceirh 45 1 Further further JJ cord-258379-v3lceirh 45 2 evidence evidence NN cord-258379-v3lceirh 45 3 for for IN cord-258379-v3lceirh 45 4 the the DT cord-258379-v3lceirh 45 5 specificity specificity NN cord-258379-v3lceirh 45 6 of of IN cord-258379-v3lceirh 45 7 the the DT cord-258379-v3lceirh 45 8 association association NN cord-258379-v3lceirh 45 9 between between IN cord-258379-v3lceirh 45 10 3c 3c NNP cord-258379-v3lceirh 45 11 and and CC cord-258379-v3lceirh 45 12 purified purify VBN cord-258379-v3lceirh 45 13 virions virion NNS cord-258379-v3lceirh 45 14 comes come VBZ cord-258379-v3lceirh 45 15 from from IN cord-258379-v3lceirh 45 16 analysis analysis NN cord-258379-v3lceirh 45 17 of of IN cord-258379-v3lceirh 45 18 the the DT cord-258379-v3lceirh 45 19 relative relative JJ cord-258379-v3lceirh 45 20 amount amount NN cord-258379-v3lceirh 45 21 of of IN cord-258379-v3lceirh 45 22 the the DT cord-258379-v3lceirh 45 23 12 12 CD cord-258379-v3lceirh 45 24 K K NNP cord-258379-v3lceirh 45 25 protein protein NN cord-258379-v3lceirh 45 26 in in IN cord-258379-v3lceirh 45 27 virus virus NN cord-258379-v3lceirh 45 28 - - HYPH cord-258379-v3lceirh 45 29 infected infect VBN cord-258379-v3lceirh 45 30 cells cell NNS cord-258379-v3lceirh 45 31 before before IN cord-258379-v3lceirh 45 32 virion virion NN cord-258379-v3lceirh 45 33 assembly assembly NN cord-258379-v3lceirh 45 34 ( ( -LRB- cord-258379-v3lceirh 45 35 Fig Fig NNP cord-258379-v3lceirh 45 36 . . . cord-258379-v3lceirh 45 37 2b 2b NNP cord-258379-v3lceirh 45 38 ) ) -RRB- cord-258379-v3lceirh 45 39 ; ; : cord-258379-v3lceirh 45 40 in in IN cord-258379-v3lceirh 45 41 this this DT cord-258379-v3lceirh 45 42 case case NN cord-258379-v3lceirh 45 43 the the DT cord-258379-v3lceirh 45 44 3c 3c NNP cord-258379-v3lceirh 45 45 protein protein NN cord-258379-v3lceirh 45 46 is be VBZ cord-258379-v3lceirh 45 47 completely completely RB cord-258379-v3lceirh 45 48 undetectable undetectable JJ cord-258379-v3lceirh 45 49 except except IN cord-258379-v3lceirh 45 50 by by IN cord-258379-v3lceirh 45 51 immunoprecipitation immunoprecipitation NN cord-258379-v3lceirh 45 52 . . . cord-258379-v3lceirh 46 1 Thus thus RB cord-258379-v3lceirh 46 2 the the DT cord-258379-v3lceirh 46 3 likelihood likelihood NN cord-258379-v3lceirh 46 4 of of IN cord-258379-v3lceirh 46 5 the the DT cord-258379-v3lceirh 46 6 3c 3c NNP cord-258379-v3lceirh 46 7 protein protein NN cord-258379-v3lceirh 46 8 appearing appear VBG cord-258379-v3lceirh 46 9 in in IN cord-258379-v3lceirh 46 10 virions virion NNS cord-258379-v3lceirh 46 11 by by IN cord-258379-v3lceirh 46 12 chance chance NN cord-258379-v3lceirh 46 13 from from IN cord-258379-v3lceirh 46 14 contaminating contaminate VBG cord-258379-v3lceirh 46 15 cellular cellular JJ cord-258379-v3lceirh 46 16 material material NN cord-258379-v3lceirh 46 17 which which WDT cord-258379-v3lceirh 46 18 has have VBZ cord-258379-v3lceirh 46 19 survived survive VBN cord-258379-v3lceirh 46 20 three three CD cord-258379-v3lceirh 46 21 cycles cycle NNS cord-258379-v3lceirh 46 22 of of IN cord-258379-v3lceirh 46 23 gradient gradient NN cord-258379-v3lceirh 46 24 purification purification NN cord-258379-v3lceirh 46 25 is be VBZ cord-258379-v3lceirh 46 26 remote remote JJ cord-258379-v3lceirh 46 27 . . . cord-258379-v3lceirh 47 1 These these DT cord-258379-v3lceirh 47 2 experiments experiment NNS cord-258379-v3lceirh 47 3 indicate indicate VBP cord-258379-v3lceirh 47 4 therefore therefore RB cord-258379-v3lceirh 47 5 that that IN cord-258379-v3lceirh 47 6 3c 3c NNP cord-258379-v3lceirh 47 7 is be VBZ cord-258379-v3lceirh 47 8 indeed indeed RB cord-258379-v3lceirh 47 9 a a DT cord-258379-v3lceirh 47 10 virion virion NN cord-258379-v3lceirh 47 11 - - HYPH cord-258379-v3lceirh 47 12 associated associate VBN cord-258379-v3lceirh 47 13 polypeptide polypeptide NN cord-258379-v3lceirh 47 14 . . . cord-258379-v3lceirh 48 1 No no DT cord-258379-v3lceirh 48 2 evidence evidence NN cord-258379-v3lceirh 48 3 was be VBD cord-258379-v3lceirh 48 4 obtained obtain VBN cord-258379-v3lceirh 48 5 , , , cord-258379-v3lceirh 48 6 however however RB cord-258379-v3lceirh 48 7 , , , cord-258379-v3lceirh 48 8 for for IN cord-258379-v3lceirh 48 9 the the DT cord-258379-v3lceirh 48 10 presence presence NN cord-258379-v3lceirh 48 11 of of IN cord-258379-v3lceirh 48 12 the the DT cord-258379-v3lceirh 48 13 other other JJ cord-258379-v3lceirh 48 14 small small JJ cord-258379-v3lceirh 48 15 virus virus NN cord-258379-v3lceirh 48 16 polypeptides polypeptide NNS cord-258379-v3lceirh 48 17 . . . cord-258379-v3lceirh 49 1 In in IN cord-258379-v3lceirh 49 2 order order NN cord-258379-v3lceirh 49 3 to to TO cord-258379-v3lceirh 49 4 assess assess VB cord-258379-v3lceirh 49 5 the the DT cord-258379-v3lceirh 49 6 approximate approximate JJ cord-258379-v3lceirh 49 7 amount amount NN cord-258379-v3lceirh 49 8 of of IN cord-258379-v3lceirh 49 9 the the DT cord-258379-v3lceirh 49 10 3c 3c NNP cord-258379-v3lceirh 49 11 polypeptide polypeptide NN cord-258379-v3lceirh 49 12 present present JJ cord-258379-v3lceirh 49 13 in in IN cord-258379-v3lceirh 49 14 the the DT cord-258379-v3lceirh 49 15 purified purify VBN cord-258379-v3lceirh 49 16 virion virion NN cord-258379-v3lceirh 49 17 preparation preparation NN cord-258379-v3lceirh 49 18 , , , cord-258379-v3lceirh 49 19 the the DT cord-258379-v3lceirh 49 20 autoradiograph autoradiograph NN cord-258379-v3lceirh 49 21 shown show VBN cord-258379-v3lceirh 49 22 in in IN cord-258379-v3lceirh 49 23 Fig Fig NNP cord-258379-v3lceirh 49 24 . . . cord-258379-v3lceirh 50 1 2a 2a LS cord-258379-v3lceirh 50 2 , , , cord-258379-v3lceirh 50 3 lane lane NN cord-258379-v3lceirh 50 4 " " `` cord-258379-v3lceirh 50 5 total total JJ cord-258379-v3lceirh 50 6 V v NN cord-258379-v3lceirh 50 7 " " '' cord-258379-v3lceirh 50 8 , , , cord-258379-v3lceirh 50 9 was be VBD cord-258379-v3lceirh 50 10 analyzed analyze VBN cord-258379-v3lceirh 50 11 by by IN cord-258379-v3lceirh 50 12 scanning scan VBG cord-258379-v3lceirh 50 13 densitometry densitometry NNP cord-258379-v3lceirh 50 14 ( ( -LRB- cord-258379-v3lceirh 50 15 Fig Fig NNP cord-258379-v3lceirh 50 16 . . . cord-258379-v3lceirh 50 17 2c 2c NNP cord-258379-v3lceirh 50 18 ) ) -RRB- cord-258379-v3lceirh 50 19 , , , cord-258379-v3lceirh 50 20 and and CC cord-258379-v3lceirh 50 21 the the DT cord-258379-v3lceirh 50 22 relative relative JJ cord-258379-v3lceirh 50 23 peak peak NN cord-258379-v3lceirh 50 24 areas area NNS cord-258379-v3lceirh 50 25 for for IN cord-258379-v3lceirh 50 26 the the DT cord-258379-v3lceirh 50 27 virion virion NN cord-258379-v3lceirh 50 28 proteins protein VBZ cord-258379-v3lceirh 50 29 NP NP NNP cord-258379-v3lceirh 50 30 , , , cord-258379-v3lceirh 50 31 M M NNP cord-258379-v3lceirh 50 32 , , , cord-258379-v3lceirh 50 33 S S NNP cord-258379-v3lceirh 50 34 , , , cord-258379-v3lceirh 50 35 and and CC cord-258379-v3lceirh 50 36 3c 3c NNP cord-258379-v3lceirh 50 37 were be VBD cord-258379-v3lceirh 50 38 measured measure VBN cord-258379-v3lceirh 50 39 . . . cord-258379-v3lceirh 51 1 Using use VBG cord-258379-v3lceirh 51 2 these these DT cord-258379-v3lceirh 51 3 data datum NNS cord-258379-v3lceirh 51 4 , , , cord-258379-v3lceirh 51 5 and and CC cord-258379-v3lceirh 51 6 taking take VBG cord-258379-v3lceirh 51 7 account account NN cord-258379-v3lceirh 51 8 of of IN cord-258379-v3lceirh 51 9 the the DT cord-258379-v3lceirh 51 10 methionine methionine NN cord-258379-v3lceirh 51 11 content content NN cord-258379-v3lceirh 51 12 of of IN cord-258379-v3lceirh 51 13 these these DT cord-258379-v3lceirh 51 14 proteins protein NNS cord-258379-v3lceirh 51 15 ( ( -LRB- cord-258379-v3lceirh 51 16 3c 3c NNP cord-258379-v3lceirh 51 17 has have VBZ cord-258379-v3lceirh 51 18 only only RB cord-258379-v3lceirh 51 19 one one CD cord-258379-v3lceirh 51 20 aside aside RB cord-258379-v3lceirh 51 21 from from IN cord-258379-v3lceirh 51 22 its -PRON- PRP$ cord-258379-v3lceirh 51 23 initiator initiator NN cord-258379-v3lceirh 51 24 , , , cord-258379-v3lceirh 51 25 and and CC cord-258379-v3lceirh 51 26 the the DT cord-258379-v3lceirh 51 27 S S NNP cord-258379-v3lceirh 51 28 , , , cord-258379-v3lceirh 51 29 NP NP NNP cord-258379-v3lceirh 51 30 , , , cord-258379-v3lceirh 51 31 and and CC cord-258379-v3lceirh 51 32 M M NNP cord-258379-v3lceirh 51 33 proteins protein NNS cord-258379-v3lceirh 51 34 have have VBP cord-258379-v3lceirh 51 35 17 17 CD cord-258379-v3lceirh 51 36 , , , cord-258379-v3lceirh 51 37 4 4 CD cord-258379-v3lceirh 51 38 , , , cord-258379-v3lceirh 51 39 and and CC cord-258379-v3lceirh 51 40 4 4 CD cord-258379-v3lceirh 51 41 , , , cord-258379-v3lceirh 51 42 respectively respectively RB cord-258379-v3lceirh 51 43 ) ) -RRB- cord-258379-v3lceirh 51 44 , , , cord-258379-v3lceirh 51 45 we -PRON- PRP cord-258379-v3lceirh 51 46 calculated calculate VBD cord-258379-v3lceirh 51 47 the the DT cord-258379-v3lceirh 51 48 relative relative JJ cord-258379-v3lceirh 51 49 molar molar JJ cord-258379-v3lceirh 51 50 proportion proportion NN cord-258379-v3lceirh 51 51 of of IN cord-258379-v3lceirh 51 52 the the DT cord-258379-v3lceirh 51 53 four four CD cord-258379-v3lceirh 51 54 proteins protein NNS cord-258379-v3lceirh 51 55 to to TO cord-258379-v3lceirh 51 56 be be VB cord-258379-v3lceirh 51 57 approximately approximately RB cord-258379-v3lceirh 51 58 1 1 CD cord-258379-v3lceirh 51 59 .O .o CD cord-258379-v3lceirh 51 60 : : : cord-258379-v3lceirh 51 61 1 1 CD cord-258379-v3lceirh 51 62 1 1 CD cord-258379-v3lceirh 51 63 .O .o CD cord-258379-v3lceirh 51 64 : : : cord-258379-v3lceirh 52 1 10.1 10.1 CD cord-258379-v3lceirh 52 2 : : : cord-258379-v3lceirh 52 3 1.7 1.7 CD cord-258379-v3lceirh 52 4 ( ( -LRB- cord-258379-v3lceirh 52 5 S s NN cord-258379-v3lceirh 52 6 : : : cord-258379-v3lceirh 52 7 NP NP NNP cord-258379-v3lceirh 52 8 : : : cord-258379-v3lceirh 52 9 M:3c M:3c NNP cord-258379-v3lceirh 52 10 ) ) -RRB- cord-258379-v3lceirh 52 11 . . . cord-258379-v3lceirh 53 1 These these DT cord-258379-v3lceirh 53 2 relative relative JJ cord-258379-v3lceirh 53 3 values value NNS cord-258379-v3lceirh 53 4 for for IN cord-258379-v3lceirh 53 5 the the DT cord-258379-v3lceirh 53 6 major major JJ cord-258379-v3lceirh 53 7 structural structural JJ cord-258379-v3lceirh 53 8 proteins protein NNS cord-258379-v3lceirh 53 9 are be VBP cord-258379-v3lceirh 53 10 broadly broadly RB cord-258379-v3lceirh 53 11 similar similar JJ cord-258379-v3lceirh 53 12 to to IN cord-258379-v3lceirh 53 13 those those DT cord-258379-v3lceirh 53 14 reported report VBN cord-258379-v3lceirh 53 15 previously previously RB cord-258379-v3lceirh 53 16 for for IN cord-258379-v3lceirh 53 17 IBV IBV NNP cord-258379-v3lceirh 53 18 ( ( -LRB- cord-258379-v3lceirh 53 19 6 6 CD cord-258379-v3lceirh 53 20 , , , cord-258379-v3lceirh 53 21 7 7 CD cord-258379-v3lceirh 53 22 ) ) -RRB- cord-258379-v3lceirh 53 23 except except IN cord-258379-v3lceirh 53 24 that that IN cord-258379-v3lceirh 53 25 the the DT cord-258379-v3lceirh 53 26 proportion proportion NN cord-258379-v3lceirh 53 27 of of IN cord-258379-v3lceirh 53 28 envelope envelope NN cord-258379-v3lceirh 53 29 proteins protein NNS cord-258379-v3lceirh 53 30 to to IN cord-258379-v3lceirh 53 31 NP NP NNP cord-258379-v3lceirh 53 32 is be VBZ cord-258379-v3lceirh 53 33 rather rather RB cord-258379-v3lceirh 53 34 lower low JJR cord-258379-v3lceirh 53 35 . . . cord-258379-v3lceirh 54 1 This this DT cord-258379-v3lceirh 54 2 calculation calculation NN cord-258379-v3lceirh 54 3 also also RB cord-258379-v3lceirh 54 4 assumes assume VBZ cord-258379-v3lceirh 54 5 that that IN cord-258379-v3lceirh 54 6 2 2 CD cord-258379-v3lceirh 54 7 . . . cord-258379-v3lceirh 55 1 ( ( -LRB- cord-258379-v3lceirh 55 2 a a LS cord-258379-v3lceirh 55 3 ) ) -RRB- cord-258379-v3lceirh 55 4 lmmunoprecipitation lmmunoprecipitation NN cord-258379-v3lceirh 55 5 of of IN cord-258379-v3lceirh 55 6 radiolabeled radiolabele VBN cord-258379-v3lceirh 55 7 virion virion NN cord-258379-v3lceirh 55 8 proteins protein NNS cord-258379-v3lceirh 55 9 with with IN cord-258379-v3lceirh 55 10 specific specific JJ cord-258379-v3lceirh 55 11 antisera antisera NN cord-258379-v3lceirh 55 12 . . . cord-258379-v3lceirh 56 1 Virions virion NNS cord-258379-v3lceirh 56 2 were be VBD cord-258379-v3lceirh 56 3 recovered recover VBN cord-258379-v3lceirh 56 4 from from IN cord-258379-v3lceirh 56 5 fractions fraction NNS cord-258379-v3lceirh 56 6 5 5 CD cord-258379-v3lceirh 56 7 , , , cord-258379-v3lceirh 56 8 6 6 CD cord-258379-v3lceirh 56 9 , , , cord-258379-v3lceirh 56 10 and and CC cord-258379-v3lceirh 56 11 7 7 CD cord-258379-v3lceirh 56 12 of of IN cord-258379-v3lceirh 56 13 the the DT cord-258379-v3lceirh 56 14 sucrose sucrose NN cord-258379-v3lceirh 56 15 density density NN cord-258379-v3lceirh 56 16 gradient gradient NN cord-258379-v3lceirh 56 17 shown show VBN cord-258379-v3lceirh 56 18 in in IN cord-258379-v3lceirh 56 19 Fig Fig NNP cord-258379-v3lceirh 56 20 . . . cord-258379-v3lceirh 57 1 1 1 CD cord-258379-v3lceirh 57 2 b b NN cord-258379-v3lceirh 57 3 as as IN cord-258379-v3lceirh 57 4 described describe VBN cord-258379-v3lceirh 57 5 in in IN cord-258379-v3lceirh 57 6 the the DT cord-258379-v3lceirh 57 7 text text NN cord-258379-v3lceirh 57 8 and and CC cord-258379-v3lceirh 57 9 immunoprecipitated immunoprecipitate VBN cord-258379-v3lceirh 57 10 with with IN cord-258379-v3lceirh 57 11 different different JJ cord-258379-v3lceirh 57 12 antisera antisera NN cord-258379-v3lceirh 57 13 as as IN cord-258379-v3lceirh 57 14 indicated indicate VBN cord-258379-v3lceirh 57 15 above above IN cord-258379-v3lceirh 57 16 each each DT cord-258379-v3lceirh 57 17 track track NN cord-258379-v3lceirh 57 18 . . . cord-258379-v3lceirh 58 1 The the DT cord-258379-v3lceirh 58 2 monospecific monospecific JJ cord-258379-v3lceirh 58 3 test test NN cord-258379-v3lceirh 58 4 antisera antisera NN cord-258379-v3lceirh 58 5 were be VBD cord-258379-v3lceirh 58 6 prepared prepare VBN cord-258379-v3lceirh 58 7 as as IN cord-258379-v3lceirh 58 8 described describe VBN cord-258379-v3lceirh 58 9 elsewhere elsewhere RB cord-258379-v3lceirh 58 10 ( ( -LRB- cord-258379-v3lceirh 58 11 17 17 CD cord-258379-v3lceirh 58 12 , , , cord-258379-v3lceirh 58 13 18 18 CD cord-258379-v3lceirh 58 14 , , , cord-258379-v3lceirh 58 15 23 23 CD cord-258379-v3lceirh 58 16 ) ) -RRB- cord-258379-v3lceirh 58 17 and and CC cord-258379-v3lceirh 58 18 control control VB cord-258379-v3lceirh 58 19 anti anti JJ cord-258379-v3lceirh 58 20 - - JJ cord-258379-v3lceirh 58 21 whole whole JJ cord-258379-v3lceirh 58 22 IBV IBV NNP cord-258379-v3lceirh 58 23 serum serum NN cord-258379-v3lceirh 58 24 was be VBD cord-258379-v3lceirh 58 25 a a DT cord-258379-v3lceirh 58 26 gift gift NN cord-258379-v3lceirh 58 27 from from IN cord-258379-v3lceirh 58 28 Dr. Dr. NNP cord-258379-v3lceirh 58 29 D. D. NNP cord-258379-v3lceirh 58 30 Cavanagh Cavanagh NNP cord-258379-v3lceirh 58 31 ( ( -LRB- cord-258379-v3lceirh 58 32 AFRC AFRC NNP cord-258379-v3lceirh 58 33 Institute Institute NNP cord-258379-v3lceirh 58 34 for for IN cord-258379-v3lceirh 58 35 Animal Animal NNP cord-258379-v3lceirh 58 36 Health Health NNP cord-258379-v3lceirh 58 37 , , , cord-258379-v3lceirh 58 38 Houghton Houghton NNP cord-258379-v3lceirh 58 39 Laboratory Laboratory NNP cord-258379-v3lceirh 58 40 , , , cord-258379-v3lceirh 58 41 UK UK NNP cord-258379-v3lceirh 58 42 ) ) -RRB- cord-258379-v3lceirh 58 43 . . . cord-258379-v3lceirh 59 1 plBT3-radiolabeled plBT3-radiolabeled NNP cord-258379-v3lceirh 59 2 3a 3a NNP cord-258379-v3lceirh 59 3 , , , cord-258379-v3lceirh 59 4 b b NNP cord-258379-v3lceirh 59 5 , , , cord-258379-v3lceirh 59 6 and and CC cord-258379-v3lceirh 59 7 c c NN cord-258379-v3lceirh 59 8 polypeptide polypeptide NN cord-258379-v3lceirh 59 9 markers marker NNS cord-258379-v3lceirh 59 10 were be VBD cord-258379-v3lceirh 59 11 prepared prepare VBN cord-258379-v3lceirh 59 12 by by IN cord-258379-v3lceirh 59 13 in in FW cord-258379-v3lceirh 59 14 vitro vitro FW cord-258379-v3lceirh 59 15 transcription transcription NN cord-258379-v3lceirh 59 16 and and CC cord-258379-v3lceirh 59 17 translation translation NN cord-258379-v3lceirh 59 18 as as IN cord-258379-v3lceirh 59 19 described describe VBN cord-258379-v3lceirh 59 20 for for IN cord-258379-v3lceirh 59 21 Fig Fig NNP cord-258379-v3lceirh 59 22 . . . cord-258379-v3lceirh 59 23 1 1 CD cord-258379-v3lceirh 59 24 a. a. NN cord-258379-v3lceirh 59 25 Total total NN cord-258379-v3lceirh 59 26 ' ' POS cord-258379-v3lceirh 59 27 V'unprecipitated v'unprecipitate VBN cord-258379-v3lceirh 59 28 virions virion NNS cord-258379-v3lceirh 59 29 . . . cord-258379-v3lceirh 60 1 Polypeptides polypeptide NNS cord-258379-v3lceirh 60 2 were be VBD cord-258379-v3lceirh 60 3 separated separate VBN cord-258379-v3lceirh 60 4 on on IN cord-258379-v3lceirh 60 5 a a DT cord-258379-v3lceirh 60 6 22 22 CD cord-258379-v3lceirh 60 7 % % NN cord-258379-v3lceirh 60 8 polyacryamide polyacryamide NN cord-258379-v3lceirh 60 9 gel gel NN cord-258379-v3lceirh 60 10 and and CC cord-258379-v3lceirh 60 11 detected detect VBN cord-258379-v3lceirh 60 12 by by IN cord-258379-v3lceirh 60 13 fluorography fluorography NNP cord-258379-v3lceirh 60 14 . . . cord-258379-v3lceirh 61 1 Lanes lane NNS cord-258379-v3lceirh 61 2 containing contain VBG cord-258379-v3lceirh 61 3 anti-3a anti-3a NNP cord-258379-v3lceirh 61 4 , , , cord-258379-v3lceirh 61 5 b b NN cord-258379-v3lceirh 61 6 , , , cord-258379-v3lceirh 61 7 and and CC cord-258379-v3lceirh 61 8 c c NN cord-258379-v3lceirh 61 9 and and CC cord-258379-v3lceirh 61 10 anti-5a anti-5a NNP cord-258379-v3lceirh 61 11 and and CC cord-258379-v3lceirh 61 12 b b NNP cord-258379-v3lceirh 61 13 antisera antisera NNP cord-258379-v3lceirh 61 14 were be VBD cord-258379-v3lceirh 61 15 exposed expose VBN cord-258379-v3lceirh 61 16 for for IN cord-258379-v3lceirh 61 17 10 10 CD cord-258379-v3lceirh 61 18 days day NNS cord-258379-v3lceirh 61 19 , , , cord-258379-v3lceirh 61 20 and and CC cord-258379-v3lceirh 61 21 other other JJ cord-258379-v3lceirh 61 22 lanes lane NNS cord-258379-v3lceirh 61 23 for for IN cord-258379-v3lceirh 61 24 2 2 CD cord-258379-v3lceirh 61 25 days day NNS cord-258379-v3lceirh 61 26 . . . cord-258379-v3lceirh 62 1 ( ( -LRB- cord-258379-v3lceirh 62 2 b b LS cord-258379-v3lceirh 62 3 ) ) -RRB- cord-258379-v3lceirh 62 4 Synthesis Synthesis NNP cord-258379-v3lceirh 62 5 of of IN cord-258379-v3lceirh 62 6 3c 3c NNP cord-258379-v3lceirh 62 7 protein protein NN cord-258379-v3lceirh 62 8 in in IN cord-258379-v3lceirh 62 9 IBV IBV NNP cord-258379-v3lceirh 62 10 - - HYPH cord-258379-v3lceirh 62 11 infected infect VBN cord-258379-v3lceirh 62 12 cells cell NNS cord-258379-v3lceirh 62 13 . . . cord-258379-v3lceirh 63 1 CK CK NNP cord-258379-v3lceirh 63 2 cells cell NNS cord-258379-v3lceirh 63 3 were be VBD cord-258379-v3lceirh 63 4 mock mock JJ cord-258379-v3lceirh 63 5 - - HYPH cord-258379-v3lceirh 63 6 infected infect VBN cord-258379-v3lceirh 63 7 or or CC cord-258379-v3lceirh 63 8 infected infect VBN cord-258379-v3lceirh 63 9 with with IN cord-258379-v3lceirh 63 10 IBV IBV NNP cord-258379-v3lceirh 63 11 and and CC cord-258379-v3lceirh 63 12 labeled label VBN cord-258379-v3lceirh 63 13 with with IN cord-258379-v3lceirh 63 14 [ [ -LRB- cord-258379-v3lceirh 63 15 ? ? . cord-258379-v3lceirh 64 1 Slmethionine Slmethionine NNP cord-258379-v3lceirh 64 2 from from IN cord-258379-v3lceirh 64 3 6 6 CD cord-258379-v3lceirh 64 4 to to IN cord-258379-v3lceirh 64 5 7.5 7.5 CD cord-258379-v3lceirh 64 6 hr hr NN cord-258379-v3lceirh 64 7 p.i p.i NNP cord-258379-v3lceirh 64 8 . . NNP cord-258379-v3lceirh 64 9 as as IN cord-258379-v3lceirh 64 10 described describe VBN cord-258379-v3lceirh 64 11 before before IN cord-258379-v3lceirh 64 12 ( ( -LRB- cord-258379-v3lceirh 64 13 23 23 CD cord-258379-v3lceirh 64 14 ) ) -RRB- cord-258379-v3lceirh 64 15 . . . cord-258379-v3lceirh 65 1 Cells cell NNS cord-258379-v3lceirh 65 2 were be VBD cord-258379-v3lceirh 65 3 harvested harvest VBN cord-258379-v3lceirh 65 4 immediately immediately RB cord-258379-v3lceirh 65 5 after after IN cord-258379-v3lceirh 65 6 labeling label VBG cord-258379-v3lceirh 65 7 and and CC cord-258379-v3lceirh 65 8 analyzed analyze VBD cord-258379-v3lceirh 65 9 on on IN cord-258379-v3lceirh 65 10 a a DT cord-258379-v3lceirh 65 11 22 22 CD cord-258379-v3lceirh 65 12 % % NN cord-258379-v3lceirh 65 13 SDS sds NN cord-258379-v3lceirh 65 14 - - HYPH cord-258379-v3lceirh 65 15 polyacrylamide polyacrylamide NN cord-258379-v3lceirh 65 16 gel gel NN cord-258379-v3lceirh 65 17 the the DT cord-258379-v3lceirh 65 18 initiator initiator NN cord-258379-v3lceirh 65 19 methionine methionine NN cord-258379-v3lceirh 65 20 is be VBZ cord-258379-v3lceirh 65 21 not not RB cord-258379-v3lceirh 65 22 removed remove VBN cord-258379-v3lceirh 65 23 from from IN cord-258379-v3lceirh 65 24 any any DT cord-258379-v3lceirh 65 25 of of IN cord-258379-v3lceirh 65 26 the the DT cord-258379-v3lceirh 65 27 proteins protein NNS cord-258379-v3lceirh 65 28 ; ; : cord-258379-v3lceirh 65 29 if if IN cord-258379-v3lceirh 65 30 one one CD cord-258379-v3lceirh 65 31 were be VBD cord-258379-v3lceirh 65 32 to to TO cord-258379-v3lceirh 65 33 assume assume VB cord-258379-v3lceirh 65 34 that that IN cord-258379-v3lceirh 65 35 cleavage cleavage NN cord-258379-v3lceirh 65 36 does do VBZ cord-258379-v3lceirh 65 37 occur occur VB cord-258379-v3lceirh 65 38 , , , cord-258379-v3lceirh 65 39 the the DT cord-258379-v3lceirh 65 40 calculated calculate VBN cord-258379-v3lceirh 65 41 relative relative JJ cord-258379-v3lceirh 65 42 proportion proportion NN cord-258379-v3lceirh 65 43 of of IN cord-258379-v3lceirh 65 44 3c 3c NNP cord-258379-v3lceirh 65 45 would would MD cord-258379-v3lceirh 65 46 increase increase VB cord-258379-v3lceirh 65 47 . . . cord-258379-v3lceirh 66 1 Figures figure NNS cord-258379-v3lceirh 66 2 are be VBP cord-258379-v3lceirh 66 3 not not RB cord-258379-v3lceirh 66 4 available available JJ cord-258379-v3lceirh 66 5 for for IN cord-258379-v3lceirh 66 6 the the DT cord-258379-v3lceirh 66 7 number number NN cord-258379-v3lceirh 66 8 of of IN cord-258379-v3lceirh 66 9 molecules molecule NNS cord-258379-v3lceirh 66 10 of of IN cord-258379-v3lceirh 66 11 each each DT cord-258379-v3lceirh 66 12 structural structural JJ cord-258379-v3lceirh 66 13 protein protein NN cord-258379-v3lceirh 66 14 present present JJ cord-258379-v3lceirh 66 15 in in IN cord-258379-v3lceirh 66 16 IBV IBV NNP cord-258379-v3lceirh 66 17 virions virion NNS cord-258379-v3lceirh 66 18 , , , cord-258379-v3lceirh 66 19 but but CC cord-258379-v3lceirh 66 20 using use VBG cord-258379-v3lceirh 66 21 those those DT cord-258379-v3lceirh 66 22 reported report VBN cord-258379-v3lceirh 66 23 for for IN cord-258379-v3lceirh 66 24 human human JJ cord-258379-v3lceirh 66 25 coronavirus coronavirus NN cord-258379-v3lceirh 66 26 OC43 OC43 NNP cord-258379-v3lceirh 66 27 as as IN cord-258379-v3lceirh 66 28 a a DT cord-258379-v3lceirh 66 29 guide guide NN cord-258379-v3lceirh 66 30 ( ( -LRB- cord-258379-v3lceirh 66 31 NP-726 NP-726 NNP cord-258379-v3lceirh 66 32 ; ; : cord-258379-v3lceirh 66 33 M-726 m-726 XX cord-258379-v3lceirh 66 34 ; ; : cord-258379-v3lceirh 66 35 S-88 S-88 NNP cord-258379-v3lceirh 66 36 ) ) -RRB- cord-258379-v3lceirh 66 37 ( ( -LRB- cord-258379-v3lceirh 66 38 12 12 CD cord-258379-v3lceirh 66 39 ) ) -RRB- cord-258379-v3lceirh 66 40 , , , cord-258379-v3lceirh 66 41 we -PRON- PRP cord-258379-v3lceirh 66 42 suggest suggest VBP cord-258379-v3lceirh 66 43 that that IN cord-258379-v3lceirh 66 44 each each DT cord-258379-v3lceirh 66 45 virion virion NN cord-258379-v3lceirh 66 46 may may MD cord-258379-v3lceirh 66 47 contain contain VB cord-258379-v3lceirh 66 48 in in IN cord-258379-v3lceirh 66 49 excess excess NN cord-258379-v3lceirh 66 50 of of IN cord-258379-v3lceirh 66 51 100 100 CD cord-258379-v3lceirh 66 52 molecules molecule NNS cord-258379-v3lceirh 66 53 of of IN cord-258379-v3lceirh 66 54 3c 3c NNP cord-258379-v3lceirh 66 55 , , , cord-258379-v3lceirh 66 56 as as RB cord-258379-v3lceirh 66 57 many many JJ cord-258379-v3lceirh 66 58 or or CC cord-258379-v3lceirh 66 59 perhaps perhaps RB cord-258379-v3lceirh 66 60 even even RB cord-258379-v3lceirh 66 61 more more JJR cord-258379-v3lceirh 66 62 than than IN cord-258379-v3lceirh 66 63 the the DT cord-258379-v3lceirh 66 64 number number NN cord-258379-v3lceirh 66 65 of of IN cord-258379-v3lceirh 66 66 spike spike NN cord-258379-v3lceirh 66 67 proteins protein NNS cord-258379-v3lceirh 66 68 . . . cord-258379-v3lceirh 67 1 This this DT cord-258379-v3lceirh 67 2 relatively relatively RB cord-258379-v3lceirh 67 3 large large JJ cord-258379-v3lceirh 67 4 proportion proportion NN cord-258379-v3lceirh 67 5 of of IN cord-258379-v3lceirh 67 6 3c 3c NNP cord-258379-v3lceirh 67 7 further further RB cord-258379-v3lceirh 67 8 supports support VBZ cord-258379-v3lceirh 67 9 the the DT cord-258379-v3lceirh 67 10 idea idea NN cord-258379-v3lceirh 67 11 that that IN cord-258379-v3lceirh 67 12 the the DT cord-258379-v3lceirh 67 13 3c 3c NNP cord-258379-v3lceirh 67 14 protein protein NN cord-258379-v3lceirh 67 15 is be VBZ cord-258379-v3lceirh 67 16 a a DT cord-258379-v3lceirh 67 17 genuine genuine JJ cord-258379-v3lceirh 67 18 virion virion NN cord-258379-v3lceirh 67 19 structural structural JJ cord-258379-v3lceirh 67 20 protein protein NN cord-258379-v3lceirh 67 21 which which WDT cord-258379-v3lceirh 67 22 may may MD cord-258379-v3lceirh 67 23 play play VB cord-258379-v3lceirh 67 24 an an DT cord-258379-v3lceirh 67 25 important important JJ cord-258379-v3lceirh 67 26 role role NN cord-258379-v3lceirh 67 27 in in IN cord-258379-v3lceirh 67 28 virion virion NN cord-258379-v3lceirh 67 29 assembly assembly NN cord-258379-v3lceirh 67 30 or or CC cord-258379-v3lceirh 67 31 infectivity infectivity NN cord-258379-v3lceirh 67 32 . . . cord-258379-v3lceirh 68 1 Since since IN cord-258379-v3lceirh 68 2 the the DT cord-258379-v3lceirh 68 3 product product NN cord-258379-v3lceirh 68 4 of of IN cord-258379-v3lceirh 68 5 the the DT cord-258379-v3lceirh 68 6 3c 3c NNP cord-258379-v3lceirh 68 7 ORF ORF NNP cord-258379-v3lceirh 68 8 is be VBZ cord-258379-v3lceirh 68 9 known know VBN cord-258379-v3lceirh 68 10 to to TO cord-258379-v3lceirh 68 11 be be VB cord-258379-v3lceirh 68 12 associated associate VBN cord-258379-v3lceirh 68 13 with with IN cord-258379-v3lceirh 68 14 the the DT cord-258379-v3lceirh 68 15 membranes membrane NNS cord-258379-v3lceirh 68 16 of of IN cord-258379-v3lceirh 68 17 infected infected JJ cord-258379-v3lceirh 68 18 cells cell NNS cord-258379-v3lceirh 68 19 ( ( -LRB- cord-258379-v3lceirh 68 20 23 23 CD cord-258379-v3lceirh 68 21 ) ) -RRB- cord-258379-v3lceirh 68 22 it -PRON- PRP cord-258379-v3lceirh 68 23 seemed seem VBD cord-258379-v3lceirh 68 24 likely likely JJ cord-258379-v3lceirh 68 25 that that IN cord-258379-v3lceirh 68 26 it -PRON- PRP cord-258379-v3lceirh 68 27 could could MD cord-258379-v3lceirh 68 28 be be VB cord-258379-v3lceirh 68 29 located locate VBN cord-258379-v3lceirh 68 30 in in IN cord-258379-v3lceirh 68 31 thevirion thevirion NNP cord-258379-v3lceirh 68 32 envelope envelope NN cord-258379-v3lceirh 68 33 . . . cord-258379-v3lceirh 69 1 To to TO cord-258379-v3lceirh 69 2 test test VB cord-258379-v3lceirh 69 3 this this DT cord-258379-v3lceirh 69 4 possibility possibility NN cord-258379-v3lceirh 69 5 , , , cord-258379-v3lceirh 69 6 a a DT cord-258379-v3lceirh 69 7 sample sample NN cord-258379-v3lceirh 69 8 of of IN cord-258379-v3lceirh 69 9 purified purify VBN cord-258379-v3lceirh 69 10 radiolabeled radiolabele VBN cord-258379-v3lceirh 69 11 virions virion NNS cord-258379-v3lceirh 69 12 purified purify VBN cord-258379-v3lceirh 69 13 by by IN cord-258379-v3lceirh 69 14 sucrose sucrose NN cord-258379-v3lceirh 69 15 and and CC cord-258379-v3lceirh 69 16 then then RB cord-258379-v3lceirh 69 17 Renografin Renografin NNP cord-258379-v3lceirh 69 18 density density NN cord-258379-v3lceirh 69 19 gradient gradient NN cord-258379-v3lceirh 69 20 sedimentations sedimentation NNS cord-258379-v3lceirh 69 21 as as IN cord-258379-v3lceirh 69 22 described describe VBN cord-258379-v3lceirh 69 23 above above RB cord-258379-v3lceirh 69 24 was be VBD cord-258379-v3lceirh 69 25 pelleted pellete VBN cord-258379-v3lceirh 69 26 bycentrifugation bycentrifugation NN cord-258379-v3lceirh 69 27 and and CC cord-258379-v3lceirh 69 28 disrupted disrupt VBD cord-258379-v3lceirh 69 29 according accord VBG cord-258379-v3lceirh 69 30 to to IN cord-258379-v3lceirh 69 31 the the DT cord-258379-v3lceirh 69 32 procedure procedure NN cord-258379-v3lceirh 69 33 of of IN cord-258379-v3lceirh 69 34 Sturman Sturman NNP cord-258379-v3lceirh 69 35 et et NNP cord-258379-v3lceirh 69 36 al al NNP cord-258379-v3lceirh 69 37 . . . cord-258379-v3lceirh 70 1 ( ( -LRB- cord-258379-v3lceirh 70 2 27 27 CD cord-258379-v3lceirh 70 3 ) ) -RRB- cord-258379-v3lceirh 70 4 by by IN cord-258379-v3lceirh 70 5 treatment treatment NN cord-258379-v3lceirh 70 6 with with IN cord-258379-v3lceirh 70 7 1 1 CD cord-258379-v3lceirh 70 8 ml ml NNS cord-258379-v3lceirh 70 9 of of IN cord-258379-v3lceirh 70 10 TNE TNE NNP cord-258379-v3lceirh 70 11 buffer buffer NN cord-258379-v3lceirh 70 12 containing contain VBG cord-258379-v3lceirh 70 13 0.25 0.25 CD cord-258379-v3lceirh 70 14 % % NN cord-258379-v3lceirh 71 1 NP-40 NP-40 NNP cord-258379-v3lceirh 71 2 . . . cord-258379-v3lceirh 72 1 The the DT cord-258379-v3lceirh 72 2 ribonucleocapsid ribonucleocapsid NN cord-258379-v3lceirh 72 3 ( ( -LRB- cord-258379-v3lceirh 72 4 RNP RNP NNP cord-258379-v3lceirh 72 5 ) ) -RRB- cord-258379-v3lceirh 72 6 and and CC cord-258379-v3lceirh 72 7 envelope envelope NN cord-258379-v3lceirh 72 8 components component NNS cord-258379-v3lceirh 72 9 of of IN cord-258379-v3lceirh 72 10 the the DT cord-258379-v3lceirh 72 11 virus virus NN cord-258379-v3lceirh 72 12 were be VBD cord-258379-v3lceirh 72 13 then then RB cord-258379-v3lceirh 72 14 separated separate VBN cord-258379-v3lceirh 72 15 by by IN cord-258379-v3lceirh 72 16 centrifugation centrifugation NN cord-258379-v3lceirh 72 17 , , , cord-258379-v3lceirh 72 18 at at IN cord-258379-v3lceirh 72 19 180,000 180,000 CD cord-258379-v3lceirh 72 20 g g NN cord-258379-v3lceirh 72 21 for for IN cord-258379-v3lceirh 72 22 18 18 CD cord-258379-v3lceirh 72 23 hr hr NN cord-258379-v3lceirh 72 24 at at IN cord-258379-v3lceirh 72 25 4 4 CD cord-258379-v3lceirh 72 26 ' ' '' cord-258379-v3lceirh 72 27 , , , cord-258379-v3lceirh 72 28 through through IN cord-258379-v3lceirh 72 29 a a DT cord-258379-v3lceirh 72 30 lo lo NNP cord-258379-v3lceirh 72 31 - - HYPH cord-258379-v3lceirh 72 32 ml ml NNP cord-258379-v3lceirh 72 33 lo-55 lo-55 NNP cord-258379-v3lceirh 72 34 % % NN cord-258379-v3lceirh 72 35 linear linear JJ cord-258379-v3lceirh 72 36 sucrose sucrose NN cord-258379-v3lceirh 72 37 gradient gradient NN cord-258379-v3lceirh 72 38 containing contain VBG cord-258379-v3lceirh 72 39 0.1 0.1 CD cord-258379-v3lceirh 72 40 % % NN cord-258379-v3lceirh 72 41 NP-40 NP-40 NNP cord-258379-v3lceirh 72 42 , , , cord-258379-v3lceirh 72 43 layered layer VBN cord-258379-v3lceirh 72 44 on on IN cord-258379-v3lceirh 72 45 top top NN cord-258379-v3lceirh 72 46 of of IN cord-258379-v3lceirh 72 47 a a DT cord-258379-v3lceirh 72 48 l l NN cord-258379-v3lceirh 72 49 - - HYPH cord-258379-v3lceirh 72 50 ml ml NN cord-258379-v3lceirh 72 51 cushion cushion NN cord-258379-v3lceirh 72 52 of of IN cord-258379-v3lceirh 72 53 75 75 CD cord-258379-v3lceirh 72 54 % % NN cord-258379-v3lceirh 72 55 Renografin Renografin NNP cord-258379-v3lceirh 72 56 also also RB cord-258379-v3lceirh 72 57 containing contain VBG cord-258379-v3lceirh 72 58 0.1 0.1 CD cord-258379-v3lceirh 72 59 % % NN cord-258379-v3lceirh 72 60 NP-40 NP-40 NNP cord-258379-v3lceirh 72 61 . . . cord-258379-v3lceirh 73 1 Eighteen eighteen CD cord-258379-v3lceirh 73 2 fractions fraction NNS cord-258379-v3lceirh 73 3 were be VBD cord-258379-v3lceirh 73 4 collected collect VBN cord-258379-v3lceirh 73 5 from from IN cord-258379-v3lceirh 73 6 the the DT cord-258379-v3lceirh 73 7 gradient gradient NN cord-258379-v3lceirh 73 8 ( ( -LRB- cord-258379-v3lceirh 73 9 of of IN cord-258379-v3lceirh 73 10 which which WDT cord-258379-v3lceirh 73 11 the the DT cord-258379-v3lceirh 73 12 first first JJ cord-258379-v3lceirh 73 13 two two CD cord-258379-v3lceirh 73 14 correspond correspond VB cord-258379-v3lceirh 73 15 to to IN cord-258379-v3lceirh 73 16 the the DT cord-258379-v3lceirh 73 17 Renografin Renografin NNP cord-258379-v3lceirh 73 18 cushion cushion NN cord-258379-v3lceirh 73 19 ) ) -RRB- cord-258379-v3lceirh 73 20 , , , cord-258379-v3lceirh 73 21 and and CC cord-258379-v3lceirh 73 22 samples sample NNS cord-258379-v3lceirh 73 23 were be VBD cord-258379-v3lceirh 73 24 analyzed analyze VBN cord-258379-v3lceirh 73 25 by by IN cord-258379-v3lceirh 73 26 SDS SDS NNP cord-258379-v3lceirh 73 27 - - HYPH cord-258379-v3lceirh 73 28 PAGE PAGE NNP cord-258379-v3lceirh 73 29 ( ( -LRB- cord-258379-v3lceirh 73 30 Fig Fig NNP cord-258379-v3lceirh 73 31 . . . cord-258379-v3lceirh 73 32 3 3 CD cord-258379-v3lceirh 73 33 ) ) -RRB- cord-258379-v3lceirh 73 34 . . . cord-258379-v3lceirh 74 1 It -PRON- PRP cord-258379-v3lceirh 74 2 can can MD cord-258379-v3lceirh 74 3 be be VB cord-258379-v3lceirh 74 4 seen see VBN cord-258379-v3lceirh 74 5 clearly clearly RB cord-258379-v3lceirh 74 6 that that IN cord-258379-v3lceirh 74 7 the the DT cord-258379-v3lceirh 74 8 major major JJ cord-258379-v3lceirh 74 9 virion virion NN cord-258379-v3lceirh 74 10 envelope envelope NN cord-258379-v3lceirh 74 11 proteins protein NNS cord-258379-v3lceirh 74 12 Sl Sl NNP cord-258379-v3lceirh 74 13 , , , cord-258379-v3lceirh 74 14 S2 s2 NN cord-258379-v3lceirh 74 15 , , , cord-258379-v3lceirh 74 16 and and CC cord-258379-v3lceirh 74 17 M M NNP cord-258379-v3lceirh 74 18 cofractionate cofractionate VBP cord-258379-v3lceirh 74 19 with with IN cord-258379-v3lceirh 74 20 each each DT cord-258379-v3lceirh 74 21 other other JJ cord-258379-v3lceirh 74 22 , , , cord-258379-v3lceirh 74 23 appearing appear VBG cord-258379-v3lceirh 74 24 maximally maximally RB cord-258379-v3lceirh 74 25 in in IN cord-258379-v3lceirh 74 26 fractions fraction NNS cord-258379-v3lceirh 74 27 15-l 15-l CD cord-258379-v3lceirh 74 28 8 8 CD cord-258379-v3lceirh 74 29 , , , cord-258379-v3lceirh 74 30 but but CC cord-258379-v3lceirh 74 31 are be VBP cord-258379-v3lceirh 74 32 well well RB cord-258379-v3lceirh 74 33 separated separate VBN cord-258379-v3lceirh 74 34 from from IN cord-258379-v3lceirh 74 35 the the DT cord-258379-v3lceirh 74 36 nucleocapsid nucleocapsid NNP cord-258379-v3lceirh 74 37 protein protein NNP cord-258379-v3lceirh 74 38 ( ( -LRB- cord-258379-v3lceirh 74 39 fractions fraction NNS cord-258379-v3lceirh 74 40 l l NN cord-258379-v3lceirh 74 41 - - HYPH cord-258379-v3lceirh 74 42 l l NN cord-258379-v3lceirh 74 43 0 0 CD cord-258379-v3lceirh 74 44 ) ) -RRB- cord-258379-v3lceirh 74 45 , , , cord-258379-v3lceirh 74 46 indicating indicate VBG cord-258379-v3lceirh 74 47 successful successful JJ cord-258379-v3lceirh 74 48 separation separation NN cord-258379-v3lceirh 74 49 of of IN cord-258379-v3lceirh 74 50 the the DT cord-258379-v3lceirh 74 51 virion virion NN cord-258379-v3lceirh 74 52 components component NNS cord-258379-v3lceirh 74 53 . . . cord-258379-v3lceirh 75 1 It -PRON- PRP cord-258379-v3lceirh 75 2 is be VBZ cord-258379-v3lceirh 75 3 also also RB cord-258379-v3lceirh 75 4 evident evident JJ cord-258379-v3lceirh 75 5 that that IN cord-258379-v3lceirh 75 6 the the DT cord-258379-v3lceirh 75 7 12 12 CD cord-258379-v3lceirh 75 8 K K NNP cord-258379-v3lceirh 75 9 virion virion NN cord-258379-v3lceirh 75 10 - - HYPH cord-258379-v3lceirh 75 11 associated associate VBN cord-258379-v3lceirh 75 12 protein protein NN cord-258379-v3lceirh 75 13 cofractionates cofractionate NNS cord-258379-v3lceirh 75 14 with with IN cord-258379-v3lceirh 75 15 the the DT cord-258379-v3lceirh 75 16 spike spike NN cord-258379-v3lceirh 75 17 and and CC cord-258379-v3lceirh 75 18 membrane membrane NN cord-258379-v3lceirh 75 19 proteins protein NNS cord-258379-v3lceirh 75 20 rather rather RB cord-258379-v3lceirh 75 21 than than IN cord-258379-v3lceirh 75 22 the the DT cord-258379-v3lceirh 75 23 nucleocapsids nucleocapsid NNS cord-258379-v3lceirh 75 24 , , , cord-258379-v3lceirh 75 25 suggesting suggest VBG cord-258379-v3lceirh 75 26 strongly strongly RB cord-258379-v3lceirh 75 27 that that IN cord-258379-v3lceirh 75 28 it -PRON- PRP cord-258379-v3lceirh 75 29 is be VBZ cord-258379-v3lceirh 75 30 indeed indeed RB cord-258379-v3lceirh 75 31 associated associate VBN cord-258379-v3lceirh 75 32 with with IN cord-258379-v3lceirh 75 33 the the DT cord-258379-v3lceirh 75 34 virion virion NN cord-258379-v3lceirh 75 35 envelope envelope NN cord-258379-v3lceirh 75 36 . . . cord-258379-v3lceirh 76 1 lmmunoprecipitation lmmunoprecipitation NN cord-258379-v3lceirh 76 2 of of IN cord-258379-v3lceirh 76 3 the the DT cord-258379-v3lceirh 76 4 pooled pool VBN cord-258379-v3lceirh 76 5 RNP RNP NNP cord-258379-v3lceirh 76 6 and and CC cord-258379-v3lceirh 76 7 envelope envelope NN cord-258379-v3lceirh 76 8 fractions fraction NNS cord-258379-v3lceirh 76 9 confirmed confirm VBD cord-258379-v3lceirh 76 10 that that IN cord-258379-v3lceirh 76 11 the the DT cord-258379-v3lceirh 76 12 12 12 CD cord-258379-v3lceirh 76 13 K K NNP cord-258379-v3lceirh 76 14 protein protein NN cord-258379-v3lceirh 76 15 present present JJ cord-258379-v3lceirh 76 16 in in IN cord-258379-v3lceirh 76 17 the the DT cord-258379-v3lceirh 76 18 envelope envelope NN cord-258379-v3lceirh 76 19 fraction fraction NN cord-258379-v3lceirh 76 20 was be VBD cord-258379-v3lceirh 76 21 indeed indeed RB cord-258379-v3lceirh 76 22 3c 3c CD cord-258379-v3lceirh 76 23 and and CC cord-258379-v3lceirh 76 24 further further RB cord-258379-v3lceirh 76 25 indicated indicate VBD cord-258379-v3lceirh 76 26 the the DT cord-258379-v3lceirh 76 27 absence absence NN cord-258379-v3lceirh 76 28 of of IN cord-258379-v3lceirh 76 29 the the DT cord-258379-v3lceirh 76 30 protein protein NN cord-258379-v3lceirh 76 31 in in IN cord-258379-v3lceirh 76 32 the the DT cord-258379-v3lceirh 76 33 RNP RNP NNP cord-258379-v3lceirh 76 34 fraction fraction NN cord-258379-v3lceirh 76 35 ( ( -LRB- cord-258379-v3lceirh 76 36 data datum NNS cord-258379-v3lceirh 76 37 not not RB cord-258379-v3lceirh 76 38 shown show VBN cord-258379-v3lceirh 76 39 ) ) -RRB- cord-258379-v3lceirh 76 40 . . . cord-258379-v3lceirh 77 1 The the DT cord-258379-v3lceirh 77 2 function function NN cord-258379-v3lceirh 77 3 of of IN cord-258379-v3lceirh 77 4 the the DT cord-258379-v3lceirh 77 5 3c 3c NNP cord-258379-v3lceirh 77 6 protein protein NN cord-258379-v3lceirh 77 7 is be VBZ cord-258379-v3lceirh 77 8 not not RB cord-258379-v3lceirh 77 9 yet yet RB cord-258379-v3lceirh 77 10 clear clear JJ cord-258379-v3lceirh 77 11 . . . cord-258379-v3lceirh 78 1 However however RB cord-258379-v3lceirh 78 2 , , , cord-258379-v3lceirh 78 3 we -PRON- PRP cord-258379-v3lceirh 78 4 are be VBP cord-258379-v3lceirh 78 5 intrigued intrigued JJ cord-258379-v3lceirh 78 6 by by IN cord-258379-v3lceirh 78 7 an an DT cord-258379-v3lceirh 78 8 overall overall JJ cord-258379-v3lceirh 78 9 similarity similarity NN cord-258379-v3lceirh 78 10 in in IN cord-258379-v3lceirh 78 11 organization organization NN cord-258379-v3lceirh 78 12 between between IN cord-258379-v3lceirh 78 13 3c 3c NNP cord-258379-v3lceirh 78 14 and and CC cord-258379-v3lceirh 78 15 the the DT cord-258379-v3lceirh 78 16 influenza influenza NN cord-258379-v3lceirh 78 17 Avirus Avirus NNP cord-258379-v3lceirh 78 18 M2 M2 NNP cord-258379-v3lceirh 78 19 protein protein NN cord-258379-v3lceirh 78 20 ( ( -LRB- cord-258379-v3lceirh 78 21 13 13 CD cord-258379-v3lceirh 78 22 , , , cord-258379-v3lceirh 78 23 74 74 CD cord-258379-v3lceirh 78 24 ) ) -RRB- cord-258379-v3lceirh 78 25 . . . cord-258379-v3lceirh 79 1 The the DT cord-258379-v3lceirh 79 2 IBV IBV NNP cord-258379-v3lceirh 79 3 3c 3c NNP cord-258379-v3lceirh 79 4 protein protein NN cord-258379-v3lceirh 79 5 is be VBZ cord-258379-v3lceirh 79 6 of of IN cord-258379-v3lceirh 79 7 a a DT cord-258379-v3lceirh 79 8 similar similar JJ cord-258379-v3lceirh 79 9 size size NN cord-258379-v3lceirh 79 10 and and CC cord-258379-v3lceirh 79 11 , , , cord-258379-v3lceirh 79 12 like like IN cord-258379-v3lceirh 79 13 M2 M2 NNP cord-258379-v3lceirh 79 14 , , , cord-258379-v3lceirh 79 15 has have VBZ cord-258379-v3lceirh 79 16 a a DT cord-258379-v3lceirh 79 17 stretch stretch NN cord-258379-v3lceirh 79 18 of of IN cord-258379-v3lceirh 79 19 hydrophobic hydrophobic JJ cord-258379-v3lceirh 79 20 amino amino NN cord-258379-v3lceirh 79 21 acids acid NNS cord-258379-v3lceirh 79 22 near near IN cord-258379-v3lceirh 79 23 its -PRON- PRP$ cord-258379-v3lceirh 79 24 N n CD cord-258379-v3lceirh 79 25 - - HYPH cord-258379-v3lceirh 79 26 terminus terminus NN cord-258379-v3lceirh 79 27 which which WDT cord-258379-v3lceirh 79 28 could could MD cord-258379-v3lceirh 79 29 span span VB cord-258379-v3lceirh 79 30 the the DT cord-258379-v3lceirh 79 31 membrane membrane NN cord-258379-v3lceirh 79 32 . . . cord-258379-v3lceirh 80 1 By by IN cord-258379-v3lceirh 80 2 analogy analogy NN cord-258379-v3lceirh 80 3 with with IN cord-258379-v3lceirh 80 4 M2 M2 NNP cord-258379-v3lceirh 80 5 , , , cord-258379-v3lceirh 80 6 which which WDT cord-258379-v3lceirh 80 7 is be VBZ cord-258379-v3lceirh 80 8 known know VBN cord-258379-v3lceirh 80 9 to to TO cord-258379-v3lceirh 80 10 be be VB cord-258379-v3lceirh 80 11 an an DT cord-258379-v3lceirh 80 12 integral integral JJ cord-258379-v3lceirh 80 13 membrane membrane NN cord-258379-v3lceirh 80 14 protein protein NN cord-258379-v3lceirh 80 15 present present JJ cord-258379-v3lceirh 80 16 in in IN cord-258379-v3lceirh 80 17 small small JJ cord-258379-v3lceirh 80 18 amounts amount NNS cord-258379-v3lceirh 80 19 in in IN cord-258379-v3lceirh 80 20 the the DT cord-258379-v3lceirh 80 21 virion virion NN cord-258379-v3lceirh 80 22 ( ( -LRB- cord-258379-v3lceirh 80 23 75 75 CD cord-258379-v3lceirh 80 24 , , , cord-258379-v3lceirh 80 25 31 31 CD cord-258379-v3lceirh 80 26 ) ) -RRB- cord-258379-v3lceirh 80 27 this this DT cord-258379-v3lceirh 80 28 would would MD cord-258379-v3lceirh 80 29 leave leave VB cord-258379-v3lceirh 80 30 the the DT cord-258379-v3lceirh 80 31 N n CD cord-258379-v3lceirh 80 32 - - HYPH cord-258379-v3lceirh 80 33 terminal terminal NN cord-258379-v3lceirh 80 34 1 1 CD cord-258379-v3lceirh 80 35 1 1 CD cord-258379-v3lceirh 80 36 amino amino NN cord-258379-v3lceirh 80 37 acids acid NNS cord-258379-v3lceirh 80 38 exposed expose VBN cord-258379-v3lceirh 80 39 on on IN cord-258379-v3lceirh 80 40 the the DT cord-258379-v3lceirh 80 41 external external JJ cord-258379-v3lceirh 80 42 surface surface NN cord-258379-v3lceirh 80 43 of of IN cord-258379-v3lceirh 80 44 the the DT cord-258379-v3lceirh 80 45 membrane membrane NN cord-258379-v3lceirh 80 46 with with IN cord-258379-v3lceirh 80 47 an an DT cord-258379-v3lceirh 80 48 internal internal JJ cord-258379-v3lceirh 80 49 C c NN cord-258379-v3lceirh 80 50 - - HYPH cord-258379-v3lceirh 80 51 terminal terminal NN cord-258379-v3lceirh 80 52 tail tail NN cord-258379-v3lceirh 80 53 of of IN cord-258379-v3lceirh 80 54 76 76 CD cord-258379-v3lceirh 80 55 residues residue NNS cord-258379-v3lceirh 80 56 . . . cord-258379-v3lceirh 81 1 Recently recently RB cord-258379-v3lceirh 81 2 it -PRON- PRP cord-258379-v3lceirh 81 3 has have VBZ cord-258379-v3lceirh 81 4 been be VBN cord-258379-v3lceirh 81 5 proposed propose VBN cord-258379-v3lceirh 81 6 that that IN cord-258379-v3lceirh 81 7 the the DT cord-258379-v3lceirh 81 8 M2 M2 NNP cord-258379-v3lceirh 81 9 protein protein NN cord-258379-v3lceirh 81 10 forms form VBZ cord-258379-v3lceirh 81 11 an an DT cord-258379-v3lceirh 81 12 ion ion NN cord-258379-v3lceirh 81 13 channel channel NN cord-258379-v3lceirh 81 14 ( ( -LRB- cord-258379-v3lceirh 81 15 28 28 CD cord-258379-v3lceirh 81 16 ) ) -RRB- cord-258379-v3lceirh 81 17 which which WDT cord-258379-v3lceirh 81 18 selves selve VBZ cord-258379-v3lceirh 81 19 to to TO cord-258379-v3lceirh 81 20 allow allow VB cord-258379-v3lceirh 81 21 both both DT cord-258379-v3lceirh 81 22 uncoating uncoating NN cord-258379-v3lceirh 81 23 of of IN cord-258379-v3lceirh 81 24 incoming incoming JJ cord-258379-v3lceirh 81 25 virions virion NNS cord-258379-v3lceirh 81 26 and and CC cord-258379-v3lceirh 81 27 proper proper JJ cord-258379-v3lceirh 81 28 assembly assembly NN cord-258379-v3lceirh 81 29 of of IN cord-258379-v3lceirh 81 30 progeny progeny JJ cord-258379-v3lceirh 81 31 virus virus NN cord-258379-v3lceirh 81 32 , , , cord-258379-v3lceirh 81 33 and and CC cord-258379-v3lceirh 81 34 this this DT cord-258379-v3lceirh 81 35 hypothesis hypothesis NN cord-258379-v3lceirh 81 36 is be VBZ cord-258379-v3lceirh 81 37 consistent consistent JJ cord-258379-v3lceirh 81 38 with with IN cord-258379-v3lceirh 81 39 the the DT cord-258379-v3lceirh 81 40 observation observation NN cord-258379-v3lceirh 81 41 that that IN cord-258379-v3lceirh 81 42 the the DT cord-258379-v3lceirh 81 43 M2 M2 NNP cord-258379-v3lceirh 81 44 protein protein NN cord-258379-v3lceirh 81 45 is be VBZ cord-258379-v3lceirh 81 46 the the DT cord-258379-v3lceirh 81 47 target target NN cord-258379-v3lceirh 81 48 for for IN cord-258379-v3lceirh 81 49 the the DT cord-258379-v3lceirh 81 50 action action NN cord-258379-v3lceirh 81 51 of of IN cord-258379-v3lceirh 81 52 the the DT cord-258379-v3lceirh 81 53 anti anti JJ cord-258379-v3lceirh 81 54 - - JJ cord-258379-v3lceirh 81 55 viral viral JJ cord-258379-v3lceirh 81 56 agent agent NN cord-258379-v3lceirh 81 57 amantadine amantadine NNP cord-258379-v3lceirh 81 58 ( ( -LRB- cord-258379-v3lceirh 81 59 10 10 CD cord-258379-v3lceirh 81 60 ) ) -RRB- cord-258379-v3lceirh 81 61 . . . cord-258379-v3lceirh 82 1 M2 M2 NNP cord-258379-v3lceirh 82 2 appears appear VBZ cord-258379-v3lceirh 82 3 to to TO cord-258379-v3lceirh 82 4 be be VB cord-258379-v3lceirh 82 5 present present JJ cord-258379-v3lceirh 82 6 in in IN cord-258379-v3lceirh 82 7 the the DT cord-258379-v3lceirh 82 8 membrane membrane NN cord-258379-v3lceirh 82 9 as as IN cord-258379-v3lceirh 82 10 a a DT cord-258379-v3lceirh 82 11 homotetramer homotetramer NN cord-258379-v3lceirh 82 12 composed compose VBN cord-258379-v3lceirh 82 13 of of IN cord-258379-v3lceirh 82 14 two two CD cord-258379-v3lceirh 82 15 disulfide disulfide RB cord-258379-v3lceirh 82 16 - - HYPH cord-258379-v3lceirh 82 17 linked link VBN cord-258379-v3lceirh 82 18 dimers dimer NNS cord-258379-v3lceirh 82 19 held hold VBN cord-258379-v3lceirh 82 20 together together RB cord-258379-v3lceirh 82 21 by by IN cord-258379-v3lceirh 82 22 noncovalent noncovalent NN cord-258379-v3lceirh 82 23 interactions interaction NNS cord-258379-v3lceirh 82 24 , , , cord-258379-v3lceirh 82 25 which which WDT cord-258379-v3lceirh 82 26 provides provide VBZ cord-258379-v3lceirh 82 27 a a DT cord-258379-v3lceirh 82 28 structural structural JJ cord-258379-v3lceirh 82 29 basis basis NN cord-258379-v3lceirh 82 30 for for IN cord-258379-v3lceirh 82 31 its -PRON- PRP$ cord-258379-v3lceirh 82 32 proposed propose VBN cord-258379-v3lceirh 82 33 function function NN cord-258379-v3lceirh 82 34 in in IN cord-258379-v3lceirh 82 35 proton proton NN cord-258379-v3lceirh 82 36 translocation translocation NN cord-258379-v3lceirh 82 37 ( ( -LRB- cord-258379-v3lceirh 82 38 28 28 CD cord-258379-v3lceirh 82 39 ) ) -RRB- cord-258379-v3lceirh 82 40 . . . cord-258379-v3lceirh 83 1 Comparison comparison NN cord-258379-v3lceirh 83 2 of of IN cord-258379-v3lceirh 83 3 the the DT cord-258379-v3lceirh 83 4 predicted predict VBN cord-258379-v3lceirh 83 5 amino amino NN cord-258379-v3lceirh 83 6 acid acid NN cord-258379-v3lceirh 83 7 sequence sequence NN cord-258379-v3lceirh 83 8 of of IN cord-258379-v3lceirh 83 9 3c 3c NNP cord-258379-v3lceirh 83 10 for for IN cord-258379-v3lceirh 83 11 five five CD cord-258379-v3lceirh 83 12 strains strain NNS cord-258379-v3lceirh 83 13 of of IN cord-258379-v3lceirh 83 14 IBV IBV NNP cord-258379-v3lceirh 83 15 ( ( -LRB- cord-258379-v3lceirh 83 16 17 17 CD cord-258379-v3lceirh 83 17 ) ) -RRB- cord-258379-v3lceirh 83 18 indicates indicate VBZ cord-258379-v3lceirh 83 19 that that IN cord-258379-v3lceirh 83 20 the the DT cord-258379-v3lceirh 83 21 hydrophobic hydrophobic JJ cord-258379-v3lceirh 83 22 character character NN cord-258379-v3lceirh 83 23 of of IN cord-258379-v3lceirh 83 24 the the DT cord-258379-v3lceirh 83 25 proposed propose VBN cord-258379-v3lceirh 83 26 transmembrane transmembrane NN cord-258379-v3lceirh 83 27 region region NN cord-258379-v3lceirh 83 28 near near IN cord-258379-v3lceirh 83 29 the the DT cord-258379-v3lceirh 83 30 N N NNP cord-258379-v3lceirh 83 31 - - HYPH cord-258379-v3lceirh 83 32 terminus terminus NN cord-258379-v3lceirh 83 33 of of IN cord-258379-v3lceirh 83 34 the the DT cord-258379-v3lceirh 83 35 protein protein NN cord-258379-v3lceirh 83 36 is be VBZ cord-258379-v3lceirh 83 37 preserved preserve VBN cord-258379-v3lceirh 83 38 , , , cord-258379-v3lceirh 83 39 although although IN cord-258379-v3lceirh 83 40 the the DT cord-258379-v3lceirh 83 41 actual actual JJ cord-258379-v3lceirh 83 42 amino amino NN cord-258379-v3lceirh 83 43 acid acid NN cord-258379-v3lceirh 83 44 sequences sequence NNS cord-258379-v3lceirh 83 45 show show VBP cord-258379-v3lceirh 83 46 slight slight JJ cord-258379-v3lceirh 83 47 differences difference NNS cord-258379-v3lceirh 83 48 . . . cord-258379-v3lceirh 84 1 There there EX cord-258379-v3lceirh 84 2 are be VBP cord-258379-v3lceirh 84 3 also also RB cord-258379-v3lceirh 84 4 two two CD cord-258379-v3lceirh 84 5 conserved conserve VBN cord-258379-v3lceirh 84 6 cysteine cysteine NN cord-258379-v3lceirh 84 7 residues residue NNS cord-258379-v3lceirh 84 8 45 45 CD cord-258379-v3lceirh 84 9 and and CC cord-258379-v3lceirh 84 10 46 46 CD cord-258379-v3lceirh 84 11 amino amino JJ cord-258379-v3lceirh 84 12 acids acid NNS cord-258379-v3lceirh 84 13 from from IN cord-258379-v3lceirh 84 14 the the DT cord-258379-v3lceirh 84 15 Nterminus Nterminus NNP cord-258379-v3lceirh 84 16 of of IN cord-258379-v3lceirh 84 17 the the DT cord-258379-v3lceirh 84 18 predicted predict VBN cord-258379-v3lceirh 84 19 sequence sequence NN cord-258379-v3lceirh 84 20 of of IN cord-258379-v3lceirh 84 21 the the DT cord-258379-v3lceirh 84 22 3c 3c NNP cord-258379-v3lceirh 84 23 products product NNS cord-258379-v3lceirh 84 24 in in IN cord-258379-v3lceirh 84 25 all all DT cord-258379-v3lceirh 84 26 five five CD cord-258379-v3lceirh 84 27 strains strain NNS cord-258379-v3lceirh 84 28 examined examine VBN cord-258379-v3lceirh 84 29 , , , cord-258379-v3lceirh 84 30 which which WDT cord-258379-v3lceirh 84 31 might may MD cord-258379-v3lceirh 84 32 serve serve VB cord-258379-v3lceirh 84 33 the the DT cord-258379-v3lceirh 84 34 same same JJ cord-258379-v3lceirh 84 35 function function NN cord-258379-v3lceirh 84 36 as as IN cord-258379-v3lceirh 84 37 cysl7 cysl7 NN cord-258379-v3lceirh 84 38 and and CC cord-258379-v3lceirh 84 39 cysl9 cysl9 NNS cord-258379-v3lceirh 84 40 of of IN cord-258379-v3lceirh 84 41 the the DT cord-258379-v3lceirh 84 42 M2 M2 NNP cord-258379-v3lceirh 84 43 protein protein NN cord-258379-v3lceirh 84 44 . . . cord-258379-v3lceirh 85 1 It -PRON- PRP cord-258379-v3lceirh 85 2 will will MD cord-258379-v3lceirh 85 3 be be VB cord-258379-v3lceirh 85 4 of of IN cord-258379-v3lceirh 85 5 considerable considerable JJ cord-258379-v3lceirh 85 6 interest interest NN cord-258379-v3lceirh 85 7 to to TO cord-258379-v3lceirh 85 8 explore explore VB cord-258379-v3lceirh 85 9 this this DT cord-258379-v3lceirh 85 10 possible possible JJ cord-258379-v3lceirh 85 11 parallel parallel NN cord-258379-v3lceirh 85 12 further far RBR cord-258379-v3lceirh 85 13 . . . cord-258379-v3lceirh 86 1 Are be VBP cord-258379-v3lceirh 86 2 3c 3c NNP cord-258379-v3lceirh 86 3 - - HYPH cord-258379-v3lceirh 86 4 like like JJ cord-258379-v3lceirh 86 5 proteins protein NNS cord-258379-v3lceirh 86 6 a a DT cord-258379-v3lceirh 86 7 general general JJ cord-258379-v3lceirh 86 8 feature feature NN cord-258379-v3lceirh 86 9 of of IN cord-258379-v3lceirh 86 10 coronaviruses coronaviruse NNS cord-258379-v3lceirh 86 11 ? ? . cord-258379-v3lceirh 87 1 On on IN cord-258379-v3lceirh 87 2 the the DT cord-258379-v3lceirh 87 3 basis basis NN cord-258379-v3lceirh 87 4 of of IN cord-258379-v3lceirh 87 5 currently currently RB cord-258379-v3lceirh 87 6 available available JJ cord-258379-v3lceirh 87 7 information information NN cord-258379-v3lceirh 87 8 , , , cord-258379-v3lceirh 87 9 this this DT cord-258379-v3lceirh 87 10 seems seem VBZ cord-258379-v3lceirh 87 11 likely likely JJ cord-258379-v3lceirh 87 12 . . . cord-258379-v3lceirh 88 1 Messenger Messenger NNP cord-258379-v3lceirh 88 2 RNA RNA NNP cord-258379-v3lceirh 88 3 4 4 CD cord-258379-v3lceirh 88 4 of of IN cord-258379-v3lceirh 88 5 mouse mouse NNP cord-258379-v3lceirh 88 6 hepatitis hepatitis NN cord-258379-v3lceirh 88 7 virus virus NN cord-258379-v3lceirh 88 8 ( ( -LRB- cord-258379-v3lceirh 88 9 MHV MHV NNP cord-258379-v3lceirh 88 10 ) ) -RRB- cord-258379-v3lceirh 88 11 is be VBZ cord-258379-v3lceirh 88 12 known know VBN cord-258379-v3lceirh 88 13 to to TO cord-258379-v3lceirh 88 14 encode encode VB cord-258379-v3lceirh 88 15 a a DT cord-258379-v3lceirh 88 16 15 15 CD cord-258379-v3lceirh 88 17 K k NN cord-258379-v3lceirh 88 18 polypeptide polypeptide NN cord-258379-v3lceirh 88 19 , , , cord-258379-v3lceirh 88 20 the the DT cord-258379-v3lceirh 88 21 predicted predict VBN cord-258379-v3lceirh 88 22 amino amino NN cord-258379-v3lceirh 88 23 acid acid NN cord-258379-v3lceirh 88 24 sequence sequence NN cord-258379-v3lceirh 88 25 of of IN cord-258379-v3lceirh 88 26 which which WDT cord-258379-v3lceirh 88 27 contains contain VBZ cord-258379-v3lceirh 88 28 a a DT cord-258379-v3lceirh 88 29 highly highly RB cord-258379-v3lceirh 88 30 hydrophobic hydrophobic JJ cord-258379-v3lceirh 88 31 region region NN cord-258379-v3lceirh 88 32 from from IN cord-258379-v3lceirh 88 33 residues residue NNS cord-258379-v3lceirh 88 34 8 8 CD cord-258379-v3lceirh 88 35 to to IN cord-258379-v3lceirh 88 36 41 41 CD cord-258379-v3lceirh 88 37 ( ( -LRB- cord-258379-v3lceirh 88 38 9 9 CD cord-258379-v3lceirh 88 39 , , , cord-258379-v3lceirh 88 40 22 22 CD cord-258379-v3lceirh 88 41 ) ) -RRB- cord-258379-v3lceirh 88 42 , , , cord-258379-v3lceirh 88 43 although although IN cord-258379-v3lceirh 88 44 the the DT cord-258379-v3lceirh 88 45 protein protein NN cord-258379-v3lceirh 88 46 has have VBZ cord-258379-v3lceirh 88 47 not not RB cord-258379-v3lceirh 88 48 so so RB cord-258379-v3lceirh 88 49 far far RB cord-258379-v3lceirh 88 50 been be VBN cord-258379-v3lceirh 88 51 found find VBN cord-258379-v3lceirh 88 52 in in IN cord-258379-v3lceirh 88 53 virions virion NNS cord-258379-v3lceirh 88 54 . . . cord-258379-v3lceirh 89 1 Wesley Wesley NNP cord-258379-v3lceirh 89 2 and and CC cord-258379-v3lceirh 89 3 Woods Woods NNP cord-258379-v3lceirh 89 4 ( ( -LRB- cord-258379-v3lceirh 89 5 29 29 CD cord-258379-v3lceirh 89 6 ) ) -RRB- cord-258379-v3lceirh 89 7 have have VBP cord-258379-v3lceirh 89 8 identified identify VBN cord-258379-v3lceirh 89 9 a a DT cord-258379-v3lceirh 89 10 17 17 CD cord-258379-v3lceirh 89 11 K K NNP cord-258379-v3lceirh 89 12 polypeptide polypeptide NN cord-258379-v3lceirh 89 13 in in IN cord-258379-v3lceirh 89 14 transmissible transmissible JJ cord-258379-v3lceirh 89 15 gastroenteritis gastroenteritis NN cord-258379-v3lceirh 89 16 virus virus NN cord-258379-v3lceirh 89 17 ( ( -LRB- cord-258379-v3lceirh 89 18 TGEV)-infected tgev)-infecte VBN cord-258379-v3lceirh 89 19 cells cell NNS cord-258379-v3lceirh 89 20 , , , cord-258379-v3lceirh 89 21 which which WDT cord-258379-v3lceirh 89 22 could could MD cord-258379-v3lceirh 89 23 be be VB cord-258379-v3lceirh 89 24 a a DT cord-258379-v3lceirh 89 25 minor minor JJ cord-258379-v3lceirh 89 26 viral viral JJ cord-258379-v3lceirh 89 27 structural structural JJ cord-258379-v3lceirh 89 28 protein protein NN cord-258379-v3lceirh 89 29 . . . cord-258379-v3lceirh 90 1 intriguingly intriguingly RB cord-258379-v3lceirh 90 2 , , , cord-258379-v3lceirh 90 3 sequence sequence NN cord-258379-v3lceirh 90 4 data datum NNS cord-258379-v3lceirh 90 5 deduced deduce VBN cord-258379-v3lceirh 90 6 from from IN cord-258379-v3lceirh 90 7 the the DT cord-258379-v3lceirh 90 8 genomic genomic JJ cord-258379-v3lceirh 90 9 RNA rna NN cord-258379-v3lceirh 90 10 of of IN cord-258379-v3lceirh 90 11 several several JJ cord-258379-v3lceirh 90 12 species specie NNS cord-258379-v3lceirh 90 13 of of IN cord-258379-v3lceirh 90 14 coronaviruses coronaviruse NNS cord-258379-v3lceirh 90 15 have have VBP cord-258379-v3lceirh 90 16 indicated indicate VBN cord-258379-v3lceirh 90 17 that that IN cord-258379-v3lceirh 90 18 the the DT cord-258379-v3lceirh 90 19 ORF ORF NNP cord-258379-v3lceirh 90 20 immediately immediately RB cord-258379-v3lceirh 90 21 upstream upstream RB cord-258379-v3lceirh 90 22 of of IN cord-258379-v3lceirh 90 23 the the DT cord-258379-v3lceirh 90 24 membrane membrane NN cord-258379-v3lceirh 90 25 protein protein NN cord-258379-v3lceirh 90 26 gene gene NN cord-258379-v3lceirh 90 27 , , , cord-258379-v3lceirh 90 28 in in IN cord-258379-v3lceirh 90 29 the the DT cord-258379-v3lceirh 90 30 equivalent equivalent JJ cord-258379-v3lceirh 90 31 position position NN cord-258379-v3lceirh 90 32 to to IN cord-258379-v3lceirh 90 33 that that DT cord-258379-v3lceirh 90 34 of of IN cord-258379-v3lceirh 90 35 3c 3c NNP cord-258379-v3lceirh 90 36 , , , cord-258379-v3lceirh 90 37 has have VBZ cord-258379-v3lceirh 90 38 the the DT cord-258379-v3lceirh 90 39 potential potential NN cord-258379-v3lceirh 90 40 to to TO cord-258379-v3lceirh 90 41 encode encode VB cord-258379-v3lceirh 90 42 a a DT cord-258379-v3lceirh 90 43 hydrophobic hydrophobic JJ cord-258379-v3lceirh 90 44 protein protein NN cord-258379-v3lceirh 90 45 with with IN cord-258379-v3lceirh 90 46 similar similar JJ cord-258379-v3lceirh 90 47 characteristics characteristic NNS cord-258379-v3lceirh 90 48 . . . cord-258379-v3lceirh 91 1 For for IN cord-258379-v3lceirh 91 2 example example NN cord-258379-v3lceirh 91 3 the the DT cord-258379-v3lceirh 91 4 second second JJ cord-258379-v3lceirh 91 5 ORF ORF NNP cord-258379-v3lceirh 91 6 ( ( -LRB- cord-258379-v3lceirh 91 7 5b 5b NN cord-258379-v3lceirh 91 8 ) ) -RRB- cord-258379-v3lceirh 91 9 of of IN cord-258379-v3lceirh 91 10 MHV MHV NNP cord-258379-v3lceirh 91 11 mRNA mrna NN cord-258379-v3lceirh 91 12 5 5 CD cord-258379-v3lceirh 91 13 can can MD cord-258379-v3lceirh 91 14 encode encode VB cord-258379-v3lceirh 91 15 a a DT cord-258379-v3lceirh 91 16 10.2 10.2 CD cord-258379-v3lceirh 91 17 K K NNP cord-258379-v3lceirh 91 18 protein protein NN cord-258379-v3lceirh 91 19 with with IN cord-258379-v3lceirh 91 20 a a DT cord-258379-v3lceirh 91 21 hydrophobic hydrophobic JJ cord-258379-v3lceirh 91 22 amino amino NN cord-258379-v3lceirh 91 23 terminal terminal NN cord-258379-v3lceirh 91 24 region region NN cord-258379-v3lceirh 91 25 ( ( -LRB- cord-258379-v3lceirh 91 26 16 16 CD cord-258379-v3lceirh 91 27 , , , cord-258379-v3lceirh 91 28 directly directly RB cord-258379-v3lceirh 91 29 or or CC cord-258379-v3lceirh 91 30 after after IN cord-258379-v3lceirh 91 31 immunoprecipitation immunoprecipitation NN cord-258379-v3lceirh 91 32 with with IN cord-258379-v3lceirh 91 33 anti-3c anti-3c NNP cord-258379-v3lceirh 91 34 antiserum antiserum NN cord-258379-v3lceirh 91 35 . . . cord-258379-v3lceirh 92 1 plBT3-radiolabeled plBT3-radiolabeled NNP cord-258379-v3lceirh 92 2 3a 3a NNP cord-258379-v3lceirh 92 3 , , , cord-258379-v3lceirh 92 4 b b NNP cord-258379-v3lceirh 92 5 , , , cord-258379-v3lceirh 92 6 and and CC cord-258379-v3lceirh 92 7 c c NN cord-258379-v3lceirh 92 8 polypeptide polypeptide NN cord-258379-v3lceirh 92 9 markers marker NNS cord-258379-v3lceirh 92 10 were be VBD cord-258379-v3lceirh 92 11 prepared prepare VBN cord-258379-v3lceirh 92 12 by by IN cord-258379-v3lceirh 92 13 in in FW cord-258379-v3lceirh 92 14 vitro vitro FW cord-258379-v3lceirh 92 15 transcription transcription NN cord-258379-v3lceirh 92 16 and and CC cord-258379-v3lceirh 92 17 translation translation NN cord-258379-v3lceirh 92 18 as as IN cord-258379-v3lceirh 92 19 described describe VBN cord-258379-v3lceirh 92 20 for for IN cord-258379-v3lceirh 92 21 Fig Fig NNP cord-258379-v3lceirh 92 22 . . NNP cord-258379-v3lceirh 92 23 1 1 CD cord-258379-v3lceirh 92 24 22 22 CD cord-258379-v3lceirh 92 25 ) ) -RRB- cord-258379-v3lceirh 92 26 and and CC cord-258379-v3lceirh 92 27 the the DT cord-258379-v3lceirh 92 28 corresponding corresponding JJ cord-258379-v3lceirh 92 29 genetic genetic JJ cord-258379-v3lceirh 92 30 location location NN cord-258379-v3lceirh 92 31 on on IN cord-258379-v3lceirh 92 32 the the DT cord-258379-v3lceirh 92 33 TGEV TGEV NNP cord-258379-v3lceirh 92 34 genome genome NN cord-258379-v3lceirh 92 35 has have VBZ cord-258379-v3lceirh 92 36 the the DT cord-258379-v3lceirh 92 37 potential potential NN cord-258379-v3lceirh 92 38 to to TO cord-258379-v3lceirh 92 39 encode encode VB cord-258379-v3lceirh 92 40 a a DT cord-258379-v3lceirh 92 41 1 1 CD cord-258379-v3lceirh 92 42 OK ok NN cord-258379-v3lceirh 92 43 polypeptide polypeptide NN cord-258379-v3lceirh 92 44 with with IN cord-258379-v3lceirh 92 45 striking strike VBG cord-258379-v3lceirh 92 46 similarity similarity NN cord-258379-v3lceirh 92 47 to to IN cord-258379-v3lceirh 92 48 3c 3c NNP cord-258379-v3lceirh 92 49 ( ( -LRB- cord-258379-v3lceirh 92 50 30 30 CD cord-258379-v3lceirh 92 51 ) ) -RRB- cord-258379-v3lceirh 92 52 . . . cord-258379-v3lceirh 93 1 Furthermore furthermore RB cord-258379-v3lceirh 93 2 , , , cord-258379-v3lceirh 93 3 the the DT cord-258379-v3lceirh 93 4 bovine bovine JJ cord-258379-v3lceirh 93 5 coronavirus coronavirus NN cord-258379-v3lceirh 93 6 genome genome NN cord-258379-v3lceirh 93 7 at at IN cord-258379-v3lceirh 93 8 this this DT cord-258379-v3lceirh 93 9 point point NN cord-258379-v3lceirh 93 10 contains contain VBZ cord-258379-v3lceirh 93 11 an an DT cord-258379-v3lceirh 93 12 ORF ORF NNP cord-258379-v3lceirh 93 13 which which WDT cord-258379-v3lceirh 93 14 could could MD cord-258379-v3lceirh 93 15 encode encode VB cord-258379-v3lceirh 93 16 a a DT cord-258379-v3lceirh 93 17 9.5 9.5 CD cord-258379-v3lceirh 93 18 K K NNP cord-258379-v3lceirh 93 19 hydrophobic hydrophobic JJ cord-258379-v3lceirh 93 20 protein protein NN cord-258379-v3lceirh 93 21 ( ( -LRB- cord-258379-v3lceirh 93 22 1 1 CD cord-258379-v3lceirh 93 23 ) ) -RRB- cord-258379-v3lceirh 93 24 . . . cord-258379-v3lceirh 94 1 Comparison comparison NN cord-258379-v3lceirh 94 2 of of IN cord-258379-v3lceirh 94 3 the the DT cord-258379-v3lceirh 94 4 predicted predict VBN cord-258379-v3lceirh 94 5 amino amino NN cord-258379-v3lceirh 94 6 acid acid NN cord-258379-v3lceirh 94 7 sequences sequence NNS cord-258379-v3lceirh 94 8 of of IN cord-258379-v3lceirh 94 9 these these DT cord-258379-v3lceirh 94 10 four four CD cord-258379-v3lceirh 94 11 proteins protein NNS cord-258379-v3lceirh 94 12 ( ( -LRB- cord-258379-v3lceirh 94 13 Fig Fig NNP cord-258379-v3lceirh 94 14 . . . cord-258379-v3lceirh 94 15 4 4 LS cord-258379-v3lceirh 94 16 ) ) -RRB- cord-258379-v3lceirh 94 17 reveals reveal VBZ cord-258379-v3lceirh 94 18 some some DT cord-258379-v3lceirh 94 19 interesting interesting JJ cord-258379-v3lceirh 94 20 common common JJ cord-258379-v3lceirh 94 21 features feature NNS cord-258379-v3lceirh 94 22 . . . cord-258379-v3lceirh 95 1 First first RB cord-258379-v3lceirh 95 2 , , , cord-258379-v3lceirh 95 3 the the DT cord-258379-v3lceirh 95 4 amino amino NN cord-258379-v3lceirh 95 5 acids acid NNS cord-258379-v3lceirh 95 6 flanking flank VBG cord-258379-v3lceirh 95 7 the the DT cord-258379-v3lceirh 95 8 proposed propose VBN cord-258379-v3lceirh 95 9 transmembrane transmembrane NN cord-258379-v3lceirh 95 10 region region NN cord-258379-v3lceirh 95 11 of of IN cord-258379-v3lceirh 95 12 the the DT cord-258379-v3lceirh 95 13 four four CD cord-258379-v3lceirh 95 14 proteins protein NNS cord-258379-v3lceirh 95 15 are be VBP cord-258379-v3lceirh 95 16 negatively negatively RB cord-258379-v3lceirh 95 17 charged charge VBN cord-258379-v3lceirh 95 18 at at IN cord-258379-v3lceirh 95 19 the the DT cord-258379-v3lceirh 95 20 N n CD cord-258379-v3lceirh 95 21 - - HYPH cord-258379-v3lceirh 95 22 terminus terminus NN cord-258379-v3lceirh 95 23 and and CC cord-258379-v3lceirh 95 24 positively positively RB cord-258379-v3lceirh 95 25 charged charge VBN cord-258379-v3lceirh 95 26 at at IN cord-258379-v3lceirh 95 27 the the DT cord-258379-v3lceirh 95 28 C C NNP cord-258379-v3lceirh 95 29 - - HYPH cord-258379-v3lceirh 95 30 terminus terminus NN cord-258379-v3lceirh 95 31 . . . cord-258379-v3lceirh 96 1 This this DT cord-258379-v3lceirh 96 2 arrangement arrangement NN cord-258379-v3lceirh 96 3 could could MD cord-258379-v3lceirh 96 4 allow allow VB cord-258379-v3lceirh 96 5 them -PRON- PRP cord-258379-v3lceirh 96 6 to to TO cord-258379-v3lceirh 96 7 adopt adopt VB cord-258379-v3lceirh 96 8 the the DT cord-258379-v3lceirh 96 9 orientation orientation NN cord-258379-v3lceirh 96 10 of of IN cord-258379-v3lceirh 96 11 class class NN cord-258379-v3lceirh 96 12 III iii CD cord-258379-v3lceirh 96 13 integral integral JJ cord-258379-v3lceirh 96 14 membrane membrane NN cord-258379-v3lceirh 96 15 proteins protein NNS cord-258379-v3lceirh 96 16 ( ( -LRB- cord-258379-v3lceirh 97 1 I -PRON- PRP cord-258379-v3lceirh 97 2 I i NN cord-258379-v3lceirh 97 3 ) ) -RRB- cord-258379-v3lceirh 97 4 , , , cord-258379-v3lceirh 97 5 i.e. i.e. FW cord-258379-v3lceirh 97 6 , , , cord-258379-v3lceirh 97 7 with with IN cord-258379-v3lceirh 97 8 the the DT cord-258379-v3lceirh 97 9 N n CD cord-258379-v3lceirh 97 10 - - HYPH cord-258379-v3lceirh 97 11 terminus terminus NN cord-258379-v3lceirh 97 12 on on IN cord-258379-v3lceirh 97 13 the the DT cord-258379-v3lceirh 97 14 outside outside NN cord-258379-v3lceirh 97 15 . . . cord-258379-v3lceirh 98 1 It -PRON- PRP cord-258379-v3lceirh 98 2 has have VBZ cord-258379-v3lceirh 98 3 also also RB cord-258379-v3lceirh 98 4 been be VBN cord-258379-v3lceirh 98 5 observed observe VBN cord-258379-v3lceirh 98 6 that that IN cord-258379-v3lceirh 98 7 the the DT cord-258379-v3lceirh 98 8 predicted predict VBN cord-258379-v3lceirh 98 9 molecular molecular JJ cord-258379-v3lceirh 98 10 weight weight NN cord-258379-v3lceirh 98 11 of of IN cord-258379-v3lceirh 98 12 the the DT cord-258379-v3lceirh 98 13 3c 3c NNP cord-258379-v3lceirh 98 14 protein protein NN cord-258379-v3lceirh 98 15 of of IN cord-258379-v3lceirh 98 16 IBV IBV NNP cord-258379-v3lceirh 98 17 , , , cord-258379-v3lceirh 98 18 the the DT cord-258379-v3lceirh 98 19 5b 5b NN cord-258379-v3lceirh 98 20 protein protein NN cord-258379-v3lceirh 98 21 of of IN cord-258379-v3lceirh 98 22 MHV MHV NNP cord-258379-v3lceirh 98 23 , , , cord-258379-v3lceirh 98 24 and and CC cord-258379-v3lceirh 98 25 the the DT cord-258379-v3lceirh 98 26 9.5 9.5 CD cord-258379-v3lceirh 98 27 K k NN cord-258379-v3lceirh 98 28 protein protein NN cord-258379-v3lceirh 98 29 of of IN cord-258379-v3lceirh 98 30 BCV BCV NNP cord-258379-v3lceirh 98 31 is be VBZ cord-258379-v3lceirh 98 32 consistent consistent JJ cord-258379-v3lceirh 98 33 with with IN cord-258379-v3lceirh 98 34 their -PRON- PRP$ cord-258379-v3lceirh 98 35 migration migration NN cord-258379-v3lceirh 98 36 rate rate NN cord-258379-v3lceirh 98 37 in in IN cord-258379-v3lceirh 98 38 SDS SDS NNP cord-258379-v3lceirh 98 39 - - HYPH cord-258379-v3lceirh 98 40 PAGE PAGE NNP cord-258379-v3lceirh 98 41 ( ( -LRB- cord-258379-v3lceirh 98 42 I -PRON- PRP cord-258379-v3lceirh 98 43 , , , cord-258379-v3lceirh 98 44 16 16 CD cord-258379-v3lceirh 98 45 , , , cord-258379-v3lceirh 98 46 17 17 CD cord-258379-v3lceirh 98 47 , , , cord-258379-v3lceirh 98 48 22 22 CD cord-258379-v3lceirh 98 49 ) ) -RRB- cord-258379-v3lceirh 98 50 suggesting suggest VBG cord-258379-v3lceirh 98 51 that that IN cord-258379-v3lceirh 98 52 these these DT cord-258379-v3lceirh 98 53 proteins protein NNS cord-258379-v3lceirh 98 54 contain contain VBP cord-258379-v3lceirh 98 55 uncleaved uncleaved JJ cord-258379-v3lceirh 98 56 signal signal NN cord-258379-v3lceirh 98 57 peptides peptide NNS cord-258379-v3lceirh 98 58 ; ; : cord-258379-v3lceirh 98 59 this this DT cord-258379-v3lceirh 98 60 is be VBZ cord-258379-v3lceirh 98 61 again again RB cord-258379-v3lceirh 98 62 consistent consistent JJ cord-258379-v3lceirh 98 63 with with IN cord-258379-v3lceirh 98 64 the the DT cord-258379-v3lceirh 98 65 possibility possibility NN cord-258379-v3lceirh 98 66 that that IN cord-258379-v3lceirh 98 67 they -PRON- PRP cord-258379-v3lceirh 98 68 are be VBP cord-258379-v3lceirh 98 69 , , , cord-258379-v3lceirh 98 70 like like IN cord-258379-v3lceirh 98 71 the the DT cord-258379-v3lceirh 98 72 M2 M2 NNP cord-258379-v3lceirh 98 73 protein protein NN cord-258379-v3lceirh 98 74 of of IN cord-258379-v3lceirh 98 75 influenza influenza NN cord-258379-v3lceirh 98 76 A a DT cord-258379-v3lceirh 98 77 virus virus NN cord-258379-v3lceirh 98 78 , , , cord-258379-v3lceirh 98 79 class class NN cord-258379-v3lceirh 98 80 III iii CD cord-258379-v3lceirh 98 81 integral integral JJ cord-258379-v3lceirh 98 82 membrane membrane NN cord-258379-v3lceirh 98 83 proteins protein NNS cord-258379-v3lceirh 98 84 ( ( -LRB- cord-258379-v3lceirh 98 85 11 11 CD cord-258379-v3lceirh 98 86 , , , cord-258379-v3lceirh 98 87 20 20 CD cord-258379-v3lceirh 98 88 ) ) -RRB- cord-258379-v3lceirh 98 89 . . . cord-258379-v3lceirh 99 1 A a DT cord-258379-v3lceirh 99 2 further further JJ cord-258379-v3lceirh 99 3 common common JJ cord-258379-v3lceirh 99 4 feature feature NN cord-258379-v3lceirh 99 5 is be VBZ cord-258379-v3lceirh 99 6 the the DT cord-258379-v3lceirh 99 7 occurrence occurrence NN cord-258379-v3lceirh 99 8 of of IN cord-258379-v3lceirh 99 9 a a DT cord-258379-v3lceirh 99 10 cysteine cysteine NN cord-258379-v3lceirh 99 11 residue residue NN cord-258379-v3lceirh 99 12 34 34 CD cord-258379-v3lceirh 99 13 or or CC cord-258379-v3lceirh 99 14 35 35 CD cord-258379-v3lceirh 99 15 nt nt RB cord-258379-v3lceirh 99 16 downstream downstream RB cord-258379-v3lceirh 99 17 of of IN cord-258379-v3lceirh 99 18 the the DT cord-258379-v3lceirh 99 19 charged charge VBN cord-258379-v3lceirh 99 20 amino amino NN cord-258379-v3lceirh 99 21 acid acid NN cord-258379-v3lceirh 99 22 at at IN cord-258379-v3lceirh 99 23 the the DT cord-258379-v3lceirh 99 24 N n CD cord-258379-v3lceirh 99 25 - - HYPH cord-258379-v3lceirh 99 26 terminal terminal JJ cord-258379-v3lceirh 99 27 end end NN cord-258379-v3lceirh 99 28 of of IN cord-258379-v3lceirh 99 29 the the DT cord-258379-v3lceirh 99 30 proposed propose VBN cord-258379-v3lceirh 99 31 transmembrane transmembrane NN cord-258379-v3lceirh 99 32 region region NN cord-258379-v3lceirh 99 33 , , , cord-258379-v3lceirh 99 34 which which WDT cord-258379-v3lceirh 99 35 may may MD cord-258379-v3lceirh 99 36 play play VB cord-258379-v3lceirh 99 37 a a DT cord-258379-v3lceirh 99 38 role role NN cord-258379-v3lceirh 99 39 , , , cord-258379-v3lceirh 99 40 as as IN cord-258379-v3lceirh 99 41 described describe VBN cord-258379-v3lceirh 99 42 above above RB cord-258379-v3lceirh 99 43 for for IN cord-258379-v3lceirh 99 44 the the DT cord-258379-v3lceirh 99 45 influenza influenza NN cord-258379-v3lceirh 99 46 M2 M2 NNP cord-258379-v3lceirh 99 47 protein protein NN cord-258379-v3lceirh 99 48 , , , cord-258379-v3lceirh 99 49 in in IN cord-258379-v3lceirh 99 50 forming form VBG cord-258379-v3lceirh 99 51 disulfide disulfide JJ cord-258379-v3lceirh 99 52 linkages linkage NNS cord-258379-v3lceirh 99 53 . . . cord-258379-v3lceirh 100 1 Thus thus RB cord-258379-v3lceirh 100 2 it -PRON- PRP cord-258379-v3lceirh 100 3 may may MD cord-258379-v3lceirh 100 4 be be VB cord-258379-v3lceirh 100 5 that that IN cord-258379-v3lceirh 100 6 3c 3c CD cord-258379-v3lceirh 100 7 - - HYPH cord-258379-v3lceirh 100 8 like like JJ cord-258379-v3lceirh 100 9 proteins protein NNS cord-258379-v3lceirh 100 10 are be VBP cord-258379-v3lceirh 100 11 conserved conserve VBN cord-258379-v3lceirh 100 12 across across IN cord-258379-v3lceirh 100 13 the the DT cord-258379-v3lceirh 100 14 Coronaviridae Coronaviridae NNP cord-258379-v3lceirh 100 15 and and CC cord-258379-v3lceirh 100 16 may may MD cord-258379-v3lceirh 100 17 play play VB cord-258379-v3lceirh 100 18 a a DT cord-258379-v3lceirh 100 19 similar similar JJ cord-258379-v3lceirh 100 20 role role NN cord-258379-v3lceirh 100 21 in in IN cord-258379-v3lceirh 100 22 viral viral JJ cord-258379-v3lceirh 100 23 replication replication NN cord-258379-v3lceirh 100 24 . . . cord-258379-v3lceirh 100 25 _SP cord-258379-v3lceirh 101 1 Proc proc VB cord-258379-v3lceirh 101 2 . . . cord-258379-v3lceirh 102 1 Nat/. Nat/. NNP cord-258379-v3lceirh 102 2 Acad Acad NNP cord-258379-v3lceirh 102 3 . . . cord-258379-v3lceirh 103 1 Sci Sci NNP cord-258379-v3lceirh 103 2 . . NNP cord-258379-v3lceirh 103 3 USA USA NNP cord-258379-v3lceirh 103 4 Ce// ce// CD cord-258379-v3lceirh 103 5 40 40 CD