id sid tid token lemma pos cord-264359-m9j3pcj1 1 1 key key JJ cord-264359-m9j3pcj1 1 2 : : : cord-264359-m9j3pcj1 1 3 cord-264359-m9j3pcj1 cord-264359-m9j3pcj1 JJ cord-264359-m9j3pcj1 1 4 authors author NNS cord-264359-m9j3pcj1 1 5 : : : cord-264359-m9j3pcj1 1 6 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 1 7 , , , cord-264359-m9j3pcj1 1 8 R. R. NNP cord-264359-m9j3pcj1 1 9 ; ; : cord-264359-m9j3pcj1 1 10 Wouters Wouters NNP cord-264359-m9j3pcj1 1 11 , , , cord-264359-m9j3pcj1 1 12 M. M. NNP cord-264359-m9j3pcj1 1 13 ; ; : cord-264359-m9j3pcj1 1 14 Boeye Boeye NNP cord-264359-m9j3pcj1 1 15 , , , cord-264359-m9j3pcj1 1 16 A. a. NN cord-264359-m9j3pcj1 1 17 title title NN cord-264359-m9j3pcj1 1 18 : : : cord-264359-m9j3pcj1 1 19 Resolution resolution NN cord-264359-m9j3pcj1 1 20 of of IN cord-264359-m9j3pcj1 1 21 the the DT cord-264359-m9j3pcj1 1 22 major major JJ cord-264359-m9j3pcj1 1 23 poliovirus poliovirus NN cord-264359-m9j3pcj1 1 24 capsid capsid NN cord-264359-m9j3pcj1 1 25 polypeptides polypeptide NNS cord-264359-m9j3pcj1 1 26 into into IN cord-264359-m9j3pcj1 1 27 doublets doublet NNS cord-264359-m9j3pcj1 1 28 date date NN cord-264359-m9j3pcj1 1 29 : : : cord-264359-m9j3pcj1 1 30 1978 1978 CD cord-264359-m9j3pcj1 1 31 - - SYM cord-264359-m9j3pcj1 1 32 05 05 CD cord-264359-m9j3pcj1 1 33 - - SYM cord-264359-m9j3pcj1 1 34 15 15 CD cord-264359-m9j3pcj1 1 35 journal journal NN cord-264359-m9j3pcj1 1 36 : : : cord-264359-m9j3pcj1 2 1 Virology virology NN cord-264359-m9j3pcj1 2 2 DOI DOI NNP cord-264359-m9j3pcj1 2 3 : : : cord-264359-m9j3pcj1 2 4 10.1016/0042 10.1016/0042 CD cord-264359-m9j3pcj1 2 5 - - HYPH cord-264359-m9j3pcj1 2 6 6822(78)90093 6822(78)90093 CD cord-264359-m9j3pcj1 2 7 - - HYPH cord-264359-m9j3pcj1 2 8 4 4 CD cord-264359-m9j3pcj1 2 9 sha sha NNP cord-264359-m9j3pcj1 2 10 : : : cord-264359-m9j3pcj1 3 1 5610c53dce08ee3038eade1231d7d6d0fc6a8ad9 5610c53dce08ee3038eade1231d7d6d0fc6a8ad9 NNP cord-264359-m9j3pcj1 3 2 doc_id doc_id CD cord-264359-m9j3pcj1 3 3 : : : cord-264359-m9j3pcj1 3 4 264359 264359 CD cord-264359-m9j3pcj1 3 5 cord_uid cord_uid NNS cord-264359-m9j3pcj1 3 6 : : : cord-264359-m9j3pcj1 4 1 m9j3pcj1 m9j3pcj1 JJ cord-264359-m9j3pcj1 5 1 Abstract Abstract NNP cord-264359-m9j3pcj1 5 2 Using use VBG cord-264359-m9j3pcj1 5 3 the the DT cord-264359-m9j3pcj1 5 4 pH pH NNP cord-264359-m9j3pcj1 5 5 gradient gradient NN cord-264359-m9j3pcj1 5 6 electrophoretic electrophoretic JJ cord-264359-m9j3pcj1 5 7 technique technique NN cord-264359-m9j3pcj1 5 8 of of IN cord-264359-m9j3pcj1 5 9 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 5 10 and and CC cord-264359-m9j3pcj1 5 11 Boeyé Boeyé NNP cord-264359-m9j3pcj1 5 12 , , , cord-264359-m9j3pcj1 5 13 the the DT cord-264359-m9j3pcj1 5 14 coat coat NN cord-264359-m9j3pcj1 5 15 protein protein NN cord-264359-m9j3pcj1 5 16 of of IN cord-264359-m9j3pcj1 5 17 poliovirus poliovirus NN cord-264359-m9j3pcj1 5 18 types type NNS cord-264359-m9j3pcj1 5 19 1 1 CD cord-264359-m9j3pcj1 5 20 and and CC cord-264359-m9j3pcj1 5 21 2 2 CD cord-264359-m9j3pcj1 5 22 was be VBD cord-264359-m9j3pcj1 5 23 resolved resolve VBN cord-264359-m9j3pcj1 5 24 into into IN cord-264359-m9j3pcj1 5 25 six six CD cord-264359-m9j3pcj1 5 26 components component NNS cord-264359-m9j3pcj1 5 27 , , , cord-264359-m9j3pcj1 5 28 Cl Cl NNP cord-264359-m9j3pcj1 5 29 to to IN cord-264359-m9j3pcj1 5 30 C6 C6 NNP cord-264359-m9j3pcj1 5 31 , , , cord-264359-m9j3pcj1 5 32 instead instead RB cord-264359-m9j3pcj1 5 33 of of IN cord-264359-m9j3pcj1 5 34 the the DT cord-264359-m9j3pcj1 5 35 classical classical JJ cord-264359-m9j3pcj1 5 36 VP1 VP1 NNP cord-264359-m9j3pcj1 5 37 - - HYPH cord-264359-m9j3pcj1 5 38 2 2 CD cord-264359-m9j3pcj1 5 39 - - SYM cord-264359-m9j3pcj1 5 40 3 3 CD cord-264359-m9j3pcj1 5 41 ( ( -LRB- cord-264359-m9j3pcj1 5 42 the the DT cord-264359-m9j3pcj1 5 43 much much RB cord-264359-m9j3pcj1 5 44 smaller small JJR cord-264359-m9j3pcj1 5 45 polypeptide polypeptide NN cord-264359-m9j3pcj1 5 46 VP4 VP4 NNP cord-264359-m9j3pcj1 5 47 was be VBD cord-264359-m9j3pcj1 5 48 excluded exclude VBN cord-264359-m9j3pcj1 5 49 from from IN cord-264359-m9j3pcj1 5 50 this this DT cord-264359-m9j3pcj1 5 51 study study NN cord-264359-m9j3pcj1 5 52 ) ) -RRB- cord-264359-m9j3pcj1 5 53 . . . cord-264359-m9j3pcj1 6 1 The the DT cord-264359-m9j3pcj1 6 2 multiple multiple JJ cord-264359-m9j3pcj1 6 3 components component NNS cord-264359-m9j3pcj1 6 4 ran run VBD cord-264359-m9j3pcj1 6 5 true true JJ cord-264359-m9j3pcj1 6 6 upon upon IN cord-264359-m9j3pcj1 6 7 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 6 8 , , , cord-264359-m9j3pcj1 6 9 and and CC cord-264359-m9j3pcj1 6 10 there there EX cord-264359-m9j3pcj1 6 11 were be VBD cord-264359-m9j3pcj1 6 12 no no DT cord-264359-m9j3pcj1 6 13 technical technical JJ cord-264359-m9j3pcj1 6 14 artifacts artifact NNS cord-264359-m9j3pcj1 6 15 . . . cord-264359-m9j3pcj1 7 1 Their -PRON- PRP$ cord-264359-m9j3pcj1 7 2 resolution resolution NN cord-264359-m9j3pcj1 7 3 did do VBD cord-264359-m9j3pcj1 7 4 not not RB cord-264359-m9j3pcj1 7 5 depend depend VB cord-264359-m9j3pcj1 7 6 on on IN cord-264359-m9j3pcj1 7 7 a a DT cord-264359-m9j3pcj1 7 8 particular particular JJ cord-264359-m9j3pcj1 7 9 method method NN cord-264359-m9j3pcj1 7 10 for for IN cord-264359-m9j3pcj1 7 11 the the DT cord-264359-m9j3pcj1 7 12 preparation preparation NN cord-264359-m9j3pcj1 7 13 or or CC cord-264359-m9j3pcj1 7 14 disruption disruption NN cord-264359-m9j3pcj1 7 15 of of IN cord-264359-m9j3pcj1 7 16 the the DT cord-264359-m9j3pcj1 7 17 virion virion NN cord-264359-m9j3pcj1 7 18 . . . cord-264359-m9j3pcj1 8 1 The the DT cord-264359-m9j3pcj1 8 2 C1–C6 c1–c6 CD cord-264359-m9j3pcj1 8 3 components component NNS cord-264359-m9j3pcj1 8 4 of of IN cord-264359-m9j3pcj1 8 5 pH pH NNP cord-264359-m9j3pcj1 8 6 gradient gradient NN cord-264359-m9j3pcj1 8 7 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 8 8 and and CC cord-264359-m9j3pcj1 8 9 the the DT cord-264359-m9j3pcj1 8 10 classical classical JJ cord-264359-m9j3pcj1 8 11 VP1–VP3 vp1–vp3 NN cord-264359-m9j3pcj1 8 12 polypeptides polypeptide NNS cord-264359-m9j3pcj1 8 13 were be VBD cord-264359-m9j3pcj1 8 14 examined examine VBN cord-264359-m9j3pcj1 8 15 with with IN cord-264359-m9j3pcj1 8 16 regard regard NN cord-264359-m9j3pcj1 8 17 to to IN cord-264359-m9j3pcj1 8 18 ( ( -LRB- cord-264359-m9j3pcj1 8 19 i i NN cord-264359-m9j3pcj1 8 20 ) ) -RRB- cord-264359-m9j3pcj1 8 21 their -PRON- PRP$ cord-264359-m9j3pcj1 8 22 migration migration NN cord-264359-m9j3pcj1 8 23 in in IN cord-264359-m9j3pcj1 8 24 normal normal JJ cord-264359-m9j3pcj1 8 25 and and CC cord-264359-m9j3pcj1 8 26 pH pH NNP cord-264359-m9j3pcj1 8 27 gradient gradient NNP cord-264359-m9j3pcj1 8 28 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 8 29 and and CC cord-264359-m9j3pcj1 8 30 ( ( -LRB- cord-264359-m9j3pcj1 8 31 ii ii NN cord-264359-m9j3pcj1 8 32 ) ) -RRB- cord-264359-m9j3pcj1 9 1 their -PRON- PRP$ cord-264359-m9j3pcj1 9 2 kinetics kinetic NNS cord-264359-m9j3pcj1 9 3 of of IN cord-264359-m9j3pcj1 9 4 leucine leucine NN cord-264359-m9j3pcj1 9 5 incorporation incorporation NN cord-264359-m9j3pcj1 9 6 in in IN cord-264359-m9j3pcj1 9 7 emetine emetine NNP cord-264359-m9j3pcj1 9 8 - - HYPH cord-264359-m9j3pcj1 9 9 stopped stop VBN cord-264359-m9j3pcj1 9 10 pulses pulse NNS cord-264359-m9j3pcj1 9 11 . . . cord-264359-m9j3pcj1 10 1 It -PRON- PRP cord-264359-m9j3pcj1 10 2 is be VBZ cord-264359-m9j3pcj1 10 3 concluded conclude VBN cord-264359-m9j3pcj1 10 4 that that IN cord-264359-m9j3pcj1 10 5 C1 C1 NNP cord-264359-m9j3pcj1 10 6 and and CC cord-264359-m9j3pcj1 10 7 C2 C2 NNP cord-264359-m9j3pcj1 10 8 were be VBD cord-264359-m9j3pcj1 10 9 both both DT cord-264359-m9j3pcj1 10 10 derived derive VBN cord-264359-m9j3pcj1 10 11 from from IN cord-264359-m9j3pcj1 10 12 VPl VPl NNP cord-264359-m9j3pcj1 10 13 , , , cord-264359-m9j3pcj1 10 14 C3 C3 NNP cord-264359-m9j3pcj1 10 15 and and CC cord-264359-m9j3pcj1 10 16 C4 c4 NN cord-264359-m9j3pcj1 10 17 from from IN cord-264359-m9j3pcj1 10 18 VP2 vp2 NN cord-264359-m9j3pcj1 10 19 , , , cord-264359-m9j3pcj1 10 20 and and CC cord-264359-m9j3pcj1 10 21 C5 C5 NNP cord-264359-m9j3pcj1 10 22 and and CC cord-264359-m9j3pcj1 10 23 C6 C6 NNP cord-264359-m9j3pcj1 10 24 from from IN cord-264359-m9j3pcj1 10 25 VP3 VP3 NNP cord-264359-m9j3pcj1 10 26 . . . cord-264359-m9j3pcj1 11 1 The the DT cord-264359-m9j3pcj1 11 2 VPl vpl NN cord-264359-m9j3pcj1 11 3 - - HYPH cord-264359-m9j3pcj1 11 4 VP3 VP3 NNP cord-264359-m9j3pcj1 11 5 group group NN cord-264359-m9j3pcj1 11 6 of of IN cord-264359-m9j3pcj1 11 7 poliovirion poliovirion NN cord-264359-m9j3pcj1 11 8 polypeptides polypeptide NNS cord-264359-m9j3pcj1 11 9 can can MD cord-264359-m9j3pcj1 11 10 be be VB cord-264359-m9j3pcj1 11 11 resolved resolve VBN cord-264359-m9j3pcj1 11 12 into into IN cord-264359-m9j3pcj1 11 13 multiple multiple JJ cord-264359-m9j3pcj1 11 14 components component NNS cord-264359-m9j3pcj1 11 15 by by IN cord-264359-m9j3pcj1 11 16 normal normal JJ cord-264359-m9j3pcj1 11 17 polyacrylamide polyacrylamide NN cord-264359-m9j3pcj1 11 18 gel gel NN cord-264359-m9j3pcj1 11 19 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 11 20 ( ( -LRB- cord-264359-m9j3pcj1 11 21 PAGE page NN cord-264359-m9j3pcj1 11 22 ) ) -RRB- cord-264359-m9j3pcj1 11 23 in in IN cord-264359-m9j3pcj1 11 24 the the DT cord-264359-m9j3pcj1 11 25 presence presence NN cord-264359-m9j3pcj1 11 26 of of IN cord-264359-m9j3pcj1 11 27 sodium sodium NN cord-264359-m9j3pcj1 11 28 dodecyl dodecyl NNP cord-264359-m9j3pcj1 11 29 sulfate sulfate NNP cord-264359-m9j3pcj1 11 30 ( ( -LRB- cord-264359-m9j3pcj1 11 31 SDS SDS NNP cord-264359-m9j3pcj1 11 32 ) ) -RRB- cord-264359-m9j3pcj1 12 1 ( ( -LRB- cord-264359-m9j3pcj1 12 2 Vanden Vanden NNP cord-264359-m9j3pcj1 12 3 Berghe Berghe NNP cord-264359-m9j3pcj1 12 4 and and CC cord-264359-m9j3pcj1 12 5 Boeye Boeye NNP cord-264359-m9j3pcj1 12 6 , , , cord-264359-m9j3pcj1 12 7 1972 1972 CD cord-264359-m9j3pcj1 12 8 ) ) -RRB- cord-264359-m9j3pcj1 12 9 ; ; : cord-264359-m9j3pcj1 12 10 however however RB cord-264359-m9j3pcj1 12 11 , , , cord-264359-m9j3pcj1 12 12 resolution resolution NN cord-264359-m9j3pcj1 12 13 into into IN cord-264359-m9j3pcj1 12 14 six six CD cord-264359-m9j3pcj1 12 15 components component NNS cord-264359-m9j3pcj1 12 16 is be VBZ cord-264359-m9j3pcj1 12 17 achieved achieve VBN cord-264359-m9j3pcj1 12 18 more more RBR cord-264359-m9j3pcj1 12 19 easily easily RB cord-264359-m9j3pcj1 12 20 and and CC cord-264359-m9j3pcj1 12 21 reproducibly reproducibly RB cord-264359-m9j3pcj1 12 22 in in IN cord-264359-m9j3pcj1 12 23 the the DT cord-264359-m9j3pcj1 12 24 presence presence NN cord-264359-m9j3pcj1 12 25 of of IN cord-264359-m9j3pcj1 12 26 a a DT cord-264359-m9j3pcj1 12 27 pH pH NNP cord-264359-m9j3pcj1 12 28 gradient gradient NN cord-264359-m9j3pcj1 12 29 . . . cord-264359-m9j3pcj1 13 1 In in IN cord-264359-m9j3pcj1 13 2 this this DT cord-264359-m9j3pcj1 13 3 paper paper NN cord-264359-m9j3pcj1 13 4 , , , cord-264359-m9j3pcj1 13 5 " " `` cord-264359-m9j3pcj1 13 6 normal normal JJ cord-264359-m9j3pcj1 13 7 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 13 8 " " '' cord-264359-m9j3pcj1 13 9 will will MD cord-264359-m9j3pcj1 13 10 refer refer VB cord-264359-m9j3pcj1 13 11 to to IN cord-264359-m9j3pcj1 13 12 SDS SDS NNP cord-264359-m9j3pcj1 13 13 - - HYPH cord-264359-m9j3pcj1 13 14 PAGE PAGE NNP cord-264359-m9j3pcj1 13 15 in in IN cord-264359-m9j3pcj1 13 16 a a DT cord-264359-m9j3pcj1 13 17 system system NN cord-264359-m9j3pcj1 13 18 entirely entirely RB cord-264359-m9j3pcj1 13 19 buffered buffer VBN cord-264359-m9j3pcj1 13 20 at at IN cord-264359-m9j3pcj1 13 21 pH pH NNP cord-264359-m9j3pcj1 13 22 7.2 7.2 CD cord-264359-m9j3pcj1 13 23 ; ; : cord-264359-m9j3pcj1 13 24 " " `` cord-264359-m9j3pcj1 13 25 pH pH NNP cord-264359-m9j3pcj1 13 26 gradient gradient NN cord-264359-m9j3pcj1 13 27 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 13 28 " " '' cord-264359-m9j3pcj1 13 29 means mean VBZ cord-264359-m9j3pcj1 13 30 that that IN cord-264359-m9j3pcj1 13 31 the the DT cord-264359-m9j3pcj1 13 32 catholyte catholyte NNP cord-264359-m9j3pcj1 13 33 was be VBD cord-264359-m9j3pcj1 13 34 maintained maintain VBN cord-264359-m9j3pcj1 13 35 at at IN cord-264359-m9j3pcj1 13 36 pH pH NNP cord-264359-m9j3pcj1 13 37 11.0 11.0 CD cord-264359-m9j3pcj1 13 38 and and CC cord-264359-m9j3pcj1 13 39 the the DT cord-264359-m9j3pcj1 13 40 anolyte anolyte NNP cord-264359-m9j3pcj1 13 41 was be VBD cord-264359-m9j3pcj1 13 42 maintained maintain VBN cord-264359-m9j3pcj1 13 43 at at IN cord-264359-m9j3pcj1 13 44 pH pH NNP cord-264359-m9j3pcj1 13 45 6.5 6.5 CD cord-264359-m9j3pcj1 13 46 . . . cord-264359-m9j3pcj1 14 1 Under under IN cord-264359-m9j3pcj1 14 2 the the DT cord-264359-m9j3pcj1 14 3 latter latter JJ cord-264359-m9j3pcj1 14 4 conditions condition NNS cord-264359-m9j3pcj1 14 5 , , , cord-264359-m9j3pcj1 14 6 the the DT cord-264359-m9j3pcj1 14 7 gel gel NN cord-264359-m9j3pcj1 14 8 ( ( -LRB- cord-264359-m9j3pcj1 14 9 initially initially RB cord-264359-m9j3pcj1 14 10 at at IN cord-264359-m9j3pcj1 14 11 pH pH NNP cord-264359-m9j3pcj1 14 12 7.2 7.2 CD cord-264359-m9j3pcj1 14 13 ) ) -RRB- cord-264359-m9j3pcj1 14 14 is be VBZ cord-264359-m9j3pcj1 14 15 rapidly rapidly RB cord-264359-m9j3pcj1 14 16 invaded invade VBN cord-264359-m9j3pcj1 14 17 by by IN cord-264359-m9j3pcj1 14 18 an an DT cord-264359-m9j3pcj1 14 19 alkaline alkaline JJ cord-264359-m9j3pcj1 14 20 zone zone NN cord-264359-m9j3pcj1 14 21 ( ( -LRB- cord-264359-m9j3pcj1 14 22 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 14 23 and and CC cord-264359-m9j3pcj1 14 24 Boeye Boeye NNP cord-264359-m9j3pcj1 14 25 , , , cord-264359-m9j3pcj1 14 26 1978 1978 CD cord-264359-m9j3pcj1 14 27 ) ) -RRB- cord-264359-m9j3pcj1 14 28 . . . cord-264359-m9j3pcj1 15 1 The the DT cord-264359-m9j3pcj1 15 2 converging converge VBG cord-264359-m9j3pcj1 15 3 results result NNS cord-264359-m9j3pcj1 15 4 of of IN cord-264359-m9j3pcj1 15 5 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 15 6 and and CC cord-264359-m9j3pcj1 15 7 emetine emetine NNP cord-264359-m9j3pcj1 15 8 - - HYPH cord-264359-m9j3pcj1 15 9 stopped stop VBN cord-264359-m9j3pcj1 15 10 pulse pulse NN cord-264359-m9j3pcj1 15 11 - - HYPH cord-264359-m9j3pcj1 15 12 labeling label VBG cord-264359-m9j3pcj1 15 13 experiments experiment NNS cord-264359-m9j3pcj1 15 14 are be VBP cord-264359-m9j3pcj1 15 15 presented present VBN cord-264359-m9j3pcj1 15 16 which which WDT cord-264359-m9j3pcj1 15 17 show show VBP cord-264359-m9j3pcj1 15 18 that that IN cord-264359-m9j3pcj1 15 19 the the DT cord-264359-m9j3pcj1 15 20 " " `` cord-264359-m9j3pcj1 15 21 classical classical JJ cord-264359-m9j3pcj1 15 22 " " '' cord-264359-m9j3pcj1 15 23 VPl vpl NN cord-264359-m9j3pcj1 15 24 , , , cord-264359-m9j3pcj1 15 25 VP2 vp2 NN cord-264359-m9j3pcj1 15 26 , , , cord-264359-m9j3pcj1 15 27 and and CC cord-264359-m9j3pcj1 15 28 VP3 VP3 NNP cord-264359-m9j3pcj1 15 29 each each DT cord-264359-m9j3pcj1 15 30 yield yield VBP cord-264359-m9j3pcj1 15 31 a a DT cord-264359-m9j3pcj1 15 32 doublet doublet NN cord-264359-m9j3pcj1 15 33 in in IN cord-264359-m9j3pcj1 15 34 pH pH NNP cord-264359-m9j3pcj1 15 35 gradient gradient NN cord-264359-m9j3pcj1 15 36 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 15 37 . . . cord-264359-m9j3pcj1 16 1 The the DT cord-264359-m9j3pcj1 16 2 two two CD cord-264359-m9j3pcj1 16 3 polypeptides polypeptide NNS cord-264359-m9j3pcj1 16 4 in in IN cord-264359-m9j3pcj1 16 5 each each DT cord-264359-m9j3pcj1 16 6 doublet doublet NN cord-264359-m9j3pcj1 16 7 appear appear VBP cord-264359-m9j3pcj1 16 8 to to TO cord-264359-m9j3pcj1 16 9 be be VB cord-264359-m9j3pcj1 16 10 nonidentical nonidentical JJ cord-264359-m9j3pcj1 16 11 , , , cord-264359-m9j3pcj1 16 12 though though IN cord-264359-m9j3pcj1 16 13 encoded encode VBN cord-264359-m9j3pcj1 16 14 by by IN cord-264359-m9j3pcj1 16 15 similar similar JJ cord-264359-m9j3pcj1 16 16 regions region NNS cord-264359-m9j3pcj1 16 17 of of IN cord-264359-m9j3pcj1 16 18 the the DT cord-264359-m9j3pcj1 16 19 viral viral JJ cord-264359-m9j3pcj1 16 20 genome genome NN cord-264359-m9j3pcj1 16 21 . . . cord-264359-m9j3pcj1 17 1 A a DT cord-264359-m9j3pcj1 17 2 preliminary preliminary JJ cord-264359-m9j3pcj1 17 3 account account NN cord-264359-m9j3pcj1 17 4 of of IN cord-264359-m9j3pcj1 17 5 these these DT cord-264359-m9j3pcj1 17 6 findings finding NNS cord-264359-m9j3pcj1 17 7 appeared appear VBD cord-264359-m9j3pcj1 17 8 elsewhere elsewhere RB cord-264359-m9j3pcj1 17 9 ( ( -LRB- cord-264359-m9j3pcj1 17 10 Boeye Boeye NNP cord-264359-m9j3pcj1 17 11 , , , cord-264359-m9j3pcj1 17 12 1976 1976 CD cord-264359-m9j3pcj1 17 13 ) ) -RRB- cord-264359-m9j3pcj1 17 14 . . . cord-264359-m9j3pcj1 17 15 ' ' '' cord-264359-m9j3pcj1 18 1 Author author NN cord-264359-m9j3pcj1 18 2 to to IN cord-264359-m9j3pcj1 18 3 whom whom WP cord-264359-m9j3pcj1 18 4 requests request VBZ cord-264359-m9j3pcj1 18 5 for for IN cord-264359-m9j3pcj1 18 6 reprints reprint NNS cord-264359-m9j3pcj1 18 7 should should MD cord-264359-m9j3pcj1 18 8 be be VB cord-264359-m9j3pcj1 18 9 addressed address VBN cord-264359-m9j3pcj1 18 10 . . . cord-264359-m9j3pcj1 19 1 Virus virus NN cord-264359-m9j3pcj1 19 2 . . . cord-264359-m9j3pcj1 20 1 Poliovirus Poliovirus NNP cord-264359-m9j3pcj1 20 2 of of IN cord-264359-m9j3pcj1 20 3 strains strain NNS cord-264359-m9j3pcj1 20 4 la la NNP cord-264359-m9j3pcj1 20 5 / / SYM cord-264359-m9j3pcj1 20 6 S3 S3 NNP cord-264359-m9j3pcj1 20 7 ( ( -LRB- cord-264359-m9j3pcj1 20 8 type type NN cord-264359-m9j3pcj1 20 9 1 1 CD cord-264359-m9j3pcj1 20 10 ) ) -RRB- cord-264359-m9j3pcj1 20 11 and and CC cord-264359-m9j3pcj1 20 12 MEF-1 MEF-1 NNP cord-264359-m9j3pcj1 21 1 ( ( -LRB- cord-264359-m9j3pcj1 21 2 type type NN cord-264359-m9j3pcj1 21 3 2 2 CD cord-264359-m9j3pcj1 21 4 ) ) -RRB- cord-264359-m9j3pcj1 21 5 was be VBD cord-264359-m9j3pcj1 21 6 grown grow VBN cord-264359-m9j3pcj1 21 7 in in IN cord-264359-m9j3pcj1 21 8 suspended suspend VBN cord-264359-m9j3pcj1 21 9 HeLa HeLa NNP cord-264359-m9j3pcj1 21 10 cells cell NNS cord-264359-m9j3pcj1 21 11 , , , cord-264359-m9j3pcj1 21 12 labeled label VBN cord-264359-m9j3pcj1 21 13 , , , cord-264359-m9j3pcj1 21 14 and and CC cord-264359-m9j3pcj1 21 15 purified purify VBN cord-264359-m9j3pcj1 21 16 as as IN cord-264359-m9j3pcj1 21 17 described describe VBN cord-264359-m9j3pcj1 21 18 ( ( -LRB- cord-264359-m9j3pcj1 21 19 Boeye Boeye NNP cord-264359-m9j3pcj1 21 20 , , , cord-264359-m9j3pcj1 21 21 1965 1965 CD cord-264359-m9j3pcj1 21 22 ; ; : cord-264359-m9j3pcj1 21 23 Vanden Vanden NNP cord-264359-m9j3pcj1 21 24 Berghe Berghe NNP cord-264359-m9j3pcj1 21 25 and and CC cord-264359-m9j3pcj1 21 26 Boeye Boeye NNP cord-264359-m9j3pcj1 21 27 , , , cord-264359-m9j3pcj1 21 28 1973 1973 CD cord-264359-m9j3pcj1 21 29 ) ) -RRB- cord-264359-m9j3pcj1 21 30 . . . cord-264359-m9j3pcj1 22 1 Extraction extraction NN cord-264359-m9j3pcj1 22 2 of of IN cord-264359-m9j3pcj1 22 3 capsidpolypeptides capsidpolypeptide NNS cord-264359-m9j3pcj1 22 4 . . . cord-264359-m9j3pcj1 23 1 A a DT cord-264359-m9j3pcj1 23 2 suspension suspension NN cord-264359-m9j3pcj1 23 3 of of IN cord-264359-m9j3pcj1 23 4 poliovirus poliovirus NN cord-264359-m9j3pcj1 23 5 in in IN cord-264359-m9j3pcj1 23 6 0.01 0.01 CD cord-264359-m9j3pcj1 23 7 M m NN cord-264359-m9j3pcj1 23 8 sodium sodium NN cord-264359-m9j3pcj1 23 9 phosphate phosphate NN cord-264359-m9j3pcj1 23 10 buffer buffer NN cord-264359-m9j3pcj1 23 11 ( ( -LRB- cord-264359-m9j3pcj1 23 12 pH pH NNP cord-264359-m9j3pcj1 23 13 7.2 7.2 CD cord-264359-m9j3pcj1 23 14 ) ) -RRB- cord-264359-m9j3pcj1 23 15 supplemented supplement VBN cord-264359-m9j3pcj1 23 16 with with IN cord-264359-m9j3pcj1 23 17 1 1 CD cord-264359-m9j3pcj1 23 18 % % NN cord-264359-m9j3pcj1 23 19 SDS sds NN cord-264359-m9j3pcj1 23 20 and and CC cord-264359-m9j3pcj1 23 21 1 1 CD cord-264359-m9j3pcj1 23 22 % % NN cord-264359-m9j3pcj1 23 23 mercaptoethanol mercaptoethanol NN cord-264359-m9j3pcj1 23 24 was be VBD cord-264359-m9j3pcj1 23 25 incubated incubate VBN cord-264359-m9j3pcj1 23 26 for for IN cord-264359-m9j3pcj1 23 27 1 1 CD cord-264359-m9j3pcj1 23 28 hr hr NN cord-264359-m9j3pcj1 23 29 at at IN cord-264359-m9j3pcj1 23 30 60 60 CD cord-264359-m9j3pcj1 23 31 " " '' cord-264359-m9j3pcj1 23 32 . . . cord-264359-m9j3pcj1 24 1 After after IN cord-264359-m9j3pcj1 24 2 cooling cool VBG cord-264359-m9j3pcj1 24 3 , , , cord-264359-m9j3pcj1 24 4 sucrose sucrose NN cord-264359-m9j3pcj1 24 5 was be VBD cord-264359-m9j3pcj1 24 6 added add VBN cord-264359-m9j3pcj1 24 7 to to IN cord-264359-m9j3pcj1 24 8 a a DT cord-264359-m9j3pcj1 24 9 final final JJ cord-264359-m9j3pcj1 24 10 concentration concentration NN cord-264359-m9j3pcj1 24 11 of of IN cord-264359-m9j3pcj1 24 12 10 10 CD cord-264359-m9j3pcj1 24 13 % % NN cord-264359-m9j3pcj1 24 14 and and CC cord-264359-m9j3pcj1 24 15 the the DT cord-264359-m9j3pcj1 24 16 mixture mixture NN cord-264359-m9j3pcj1 24 17 was be VBD cord-264359-m9j3pcj1 24 18 electrophoresed electrophoresed JJ cord-264359-m9j3pcj1 24 19 immediately immediately RB cord-264359-m9j3pcj1 24 20 . . . cord-264359-m9j3pcj1 25 1 Gels gel NNS cord-264359-m9j3pcj1 25 2 and and CC cord-264359-m9j3pcj1 25 3 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 25 4 . . . cord-264359-m9j3pcj1 26 1 All all DT cord-264359-m9j3pcj1 26 2 procedures procedure NNS cord-264359-m9j3pcj1 26 3 were be VBD cord-264359-m9j3pcj1 26 4 as as IN cord-264359-m9j3pcj1 26 5 described describe VBN cord-264359-m9j3pcj1 26 6 by by IN cord-264359-m9j3pcj1 26 7 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 26 8 and and CC cord-264359-m9j3pcj1 26 9 Boeye Boeye NNP cord-264359-m9j3pcj1 26 10 ( ( -LRB- cord-264359-m9j3pcj1 26 11 1978 1978 CD cord-264359-m9j3pcj1 26 12 ) ) -RRB- cord-264359-m9j3pcj1 26 13 . . . cord-264359-m9j3pcj1 27 1 The the DT cord-264359-m9j3pcj1 27 2 length length NN cord-264359-m9j3pcj1 27 3 of of IN cord-264359-m9j3pcj1 27 4 the the DT cord-264359-m9j3pcj1 27 5 gels gel NNS cord-264359-m9j3pcj1 27 6 was be VBD cord-264359-m9j3pcj1 27 7 9 9 CD cord-264359-m9j3pcj1 27 8 cm cm NNS cord-264359-m9j3pcj1 27 9 and and CC cord-264359-m9j3pcj1 27 10 the the DT cord-264359-m9j3pcj1 27 11 potential potential NN cord-264359-m9j3pcj1 27 12 was be VBD cord-264359-m9j3pcj1 27 13 25 25 CD cord-264359-m9j3pcj1 27 14 V. V. NNP cord-264359-m9j3pcj1 27 15 Radioactivity Radioactivity NNP cord-264359-m9j3pcj1 27 16 profiles profile NNS cord-264359-m9j3pcj1 27 17 . . . cord-264359-m9j3pcj1 28 1 Gels gel NNS cord-264359-m9j3pcj1 28 2 were be VBD cord-264359-m9j3pcj1 28 3 cut cut VBN cord-264359-m9j3pcj1 28 4 into into IN cord-264359-m9j3pcj1 28 5 l l NN cord-264359-m9j3pcj1 28 6 - - HYPH cord-264359-m9j3pcj1 28 7 mm mm NNP cord-264359-m9j3pcj1 28 8 slices slice NNS cord-264359-m9j3pcj1 28 9 , , , cord-264359-m9j3pcj1 28 10 and and CC cord-264359-m9j3pcj1 28 11 these these DT cord-264359-m9j3pcj1 28 12 were be VBD cord-264359-m9j3pcj1 28 13 either either RB cord-264359-m9j3pcj1 28 14 counted count VBN cord-264359-m9j3pcj1 28 15 after after IN cord-264359-m9j3pcj1 28 16 adding add VBG cord-264359-m9j3pcj1 28 17 soluene soluene NN cord-264359-m9j3pcj1 28 18 and and CC cord-264359-m9j3pcj1 28 19 a a DT cord-264359-m9j3pcj1 28 20 toluene toluene NN cord-264359-m9j3pcj1 28 21 - - HYPH cord-264359-m9j3pcj1 28 22 based base VBN cord-264359-m9j3pcj1 28 23 scintillation scintillation NN cord-264359-m9j3pcj1 28 24 mixture mixture NN cord-264359-m9j3pcj1 28 25 or or CC cord-264359-m9j3pcj1 28 26 eluted elute VBN cord-264359-m9j3pcj1 28 27 for for IN cord-264359-m9j3pcj1 28 28 24 24 CD cord-264359-m9j3pcj1 28 29 hr hr NN cord-264359-m9j3pcj1 28 30 at at IN cord-264359-m9j3pcj1 28 31 room room NN cord-264359-m9j3pcj1 28 32 temperature temperature NN cord-264359-m9j3pcj1 28 33 in in IN cord-264359-m9j3pcj1 28 34 0.3 0.3 CD cord-264359-m9j3pcj1 28 35 to to IN cord-264359-m9j3pcj1 28 36 0.5 0.5 CD cord-264359-m9j3pcj1 28 37 ml ml NNS cord-264359-m9j3pcj1 28 38 of of IN cord-264359-m9j3pcj1 28 39 0.1 0.1 CD cord-264359-m9j3pcj1 28 40 % % NN cord-264359-m9j3pcj1 28 41 SDS SDS NNP cord-264359-m9j3pcj1 28 42 . . . cord-264359-m9j3pcj1 29 1 Eluate eluate JJ cord-264359-m9j3pcj1 29 2 samples sample NNS cord-264359-m9j3pcj1 29 3 of of IN cord-264359-m9j3pcj1 29 4 20 20 CD cord-264359-m9j3pcj1 29 5 ~1 ~1 NFP cord-264359-m9j3pcj1 29 6 were be VBD cord-264359-m9j3pcj1 29 7 counted count VBN cord-264359-m9j3pcj1 29 8 in in IN cord-264359-m9j3pcj1 29 9 Bray Bray NNP cord-264359-m9j3pcj1 29 10 's 's POS cord-264359-m9j3pcj1 29 11 mixture mixture NN cord-264359-m9j3pcj1 29 12 . . . cord-264359-m9j3pcj1 30 1 Normal normal JJ cord-264359-m9j3pcj1 30 2 counting counting NN cord-264359-m9j3pcj1 30 3 time time NN cord-264359-m9j3pcj1 30 4 was be VBD cord-264359-m9j3pcj1 30 5 10 10 CD cord-264359-m9j3pcj1 30 6 min min NN cord-264359-m9j3pcj1 30 7 , , , cord-264359-m9j3pcj1 30 8 but but CC cord-264359-m9j3pcj1 30 9 this this DT cord-264359-m9j3pcj1 30 10 was be VBD cord-264359-m9j3pcj1 30 11 increased increase VBN cord-264359-m9j3pcj1 30 12 to to IN cord-264359-m9j3pcj1 30 13 200 200 CD cord-264359-m9j3pcj1 30 14 min min NN cord-264359-m9j3pcj1 30 15 as as IN cord-264359-m9j3pcj1 30 16 needed need VBN cord-264359-m9j3pcj1 30 17 . . . cord-264359-m9j3pcj1 31 1 When when WRB cord-264359-m9j3pcj1 31 2 required require VBN cord-264359-m9j3pcj1 31 3 , , , cord-264359-m9j3pcj1 31 4 eluate eluate JJ cord-264359-m9j3pcj1 31 5 pools pool NNS cord-264359-m9j3pcj1 31 6 were be VBD cord-264359-m9j3pcj1 31 7 concentrated concentrate VBN cord-264359-m9j3pcj1 31 8 by by IN cord-264359-m9j3pcj1 31 9 ultrafiltration ultrafiltration NN cord-264359-m9j3pcj1 31 10 in in IN cord-264359-m9j3pcj1 31 11 a a DT cord-264359-m9j3pcj1 31 12 Minicon Minicon NNP cord-264359-m9j3pcj1 31 13 B-15 B-15 NNP cord-264359-m9j3pcj1 31 14 ( ( -LRB- cord-264359-m9j3pcj1 31 15 Amicon Amicon NNP cord-264359-m9j3pcj1 31 16 ) ) -RRB- cord-264359-m9j3pcj1 31 17 apparatus apparatus NN cord-264359-m9j3pcj1 31 18 . . . cord-264359-m9j3pcj1 32 1 For for IN cord-264359-m9j3pcj1 32 2 each each DT cord-264359-m9j3pcj1 32 3 series series NN cord-264359-m9j3pcj1 32 4 of of IN cord-264359-m9j3pcj1 32 5 radioactive radioactive JJ cord-264359-m9j3pcj1 32 6 profiles profile NNS cord-264359-m9j3pcj1 32 7 , , , cord-264359-m9j3pcj1 32 8 one one CD cord-264359-m9j3pcj1 32 9 gel gel NN cord-264359-m9j3pcj1 32 10 loaded load VBN cord-264359-m9j3pcj1 32 11 with with IN cord-264359-m9j3pcj1 32 12 cold cold JJ cord-264359-m9j3pcj1 32 13 la la NNP cord-264359-m9j3pcj1 32 14 / / SYM cord-264359-m9j3pcj1 32 15 S3 S3 NNP cord-264359-m9j3pcj1 32 16 protein protein NN cord-264359-m9j3pcj1 32 17 was be VBD cord-264359-m9j3pcj1 32 18 Incorporation Incorporation NNP cord-264359-m9j3pcj1 32 19 studies study NNS cord-264359-m9j3pcj1 32 20 using use VBG cord-264359-m9j3pcj1 32 21 enietine enietine NN cord-264359-m9j3pcj1 32 22 ( ( -LRB- cord-264359-m9j3pcj1 32 23 modified modify VBN cord-264359-m9j3pcj1 32 24 from from IN cord-264359-m9j3pcj1 32 25 Rekosh Rekosh NNP cord-264359-m9j3pcj1 32 26 , , , cord-264359-m9j3pcj1 32 27 1972 1972 CD cord-264359-m9j3pcj1 32 28 ) ) -RRB- cord-264359-m9j3pcj1 32 29 . . . cord-264359-m9j3pcj1 33 1 HeLa HeLa NNP cord-264359-m9j3pcj1 33 2 cells cell NNS cord-264359-m9j3pcj1 33 3 were be VBD cord-264359-m9j3pcj1 33 4 collected collect VBN cord-264359-m9j3pcj1 33 5 3 3 CD cord-264359-m9j3pcj1 33 6 hr hr NN cord-264359-m9j3pcj1 33 7 after after IN cord-264359-m9j3pcj1 33 8 high high JJ cord-264359-m9j3pcj1 33 9 - - HYPH cord-264359-m9j3pcj1 33 10 multiplicity multiplicity NN cord-264359-m9j3pcj1 33 11 infection infection NN cord-264359-m9j3pcj1 33 12 and and CC cord-264359-m9j3pcj1 33 13 brought bring VBD cord-264359-m9j3pcj1 33 14 to to IN cord-264359-m9j3pcj1 33 15 a a DT cord-264359-m9j3pcj1 33 16 density density NN cord-264359-m9j3pcj1 33 17 of of IN cord-264359-m9j3pcj1 33 18 4 4 CD cord-264359-m9j3pcj1 33 19 X x SYM cord-264359-m9j3pcj1 33 20 10 10 CD cord-264359-m9j3pcj1 33 21 " " `` cord-264359-m9j3pcj1 33 22 cells cell NNS cord-264359-m9j3pcj1 33 23 / / SYM cord-264359-m9j3pcj1 33 24 ml ml NN cord-264359-m9j3pcj1 33 25 at at IN cord-264359-m9j3pcj1 33 26 37 37 CD cord-264359-m9j3pcj1 33 27 " " `` cord-264359-m9j3pcj1 33 28 in in IN cord-264359-m9j3pcj1 33 29 Eagle Eagle NNP cord-264359-m9j3pcj1 33 30 's 's POS cord-264359-m9j3pcj1 33 31 medium medium JJ cord-264359-m9j3pcj1 33 32 devoid devoid NN cord-264359-m9j3pcj1 33 33 of of IN cord-264359-m9j3pcj1 33 34 amino amino JJ cord-264359-m9j3pcj1 33 35 acids acid NNS cord-264359-m9j3pcj1 33 36 . . . cord-264359-m9j3pcj1 34 1 r3H]Leucine r3H]Leucine NNP cord-264359-m9j3pcj1 34 2 was be VBD cord-264359-m9j3pcj1 34 3 added add VBN cord-264359-m9j3pcj1 34 4 15 15 CD cord-264359-m9j3pcj1 34 5 min min NN cord-264359-m9j3pcj1 34 6 later later RB cord-264359-m9j3pcj1 34 7 , , , cord-264359-m9j3pcj1 34 8 and and CC cord-264359-m9j3pcj1 34 9 its -PRON- PRP$ cord-264359-m9j3pcj1 34 10 incorporation incorporation NN cord-264359-m9j3pcj1 34 11 was be VBD cord-264359-m9j3pcj1 34 12 completely completely RB cord-264359-m9j3pcj1 34 13 stopped stop VBN cord-264359-m9j3pcj1 34 14 after after IN cord-264359-m9j3pcj1 34 15 a a DT cord-264359-m9j3pcj1 34 16 short short JJ cord-264359-m9j3pcj1 34 17 time time NN cord-264359-m9j3pcj1 34 18 ( ( -LRB- cord-264359-m9j3pcj1 34 19 to to TO cord-264359-m9j3pcj1 34 20 be be VB cord-264359-m9j3pcj1 34 21 specified specify VBN cord-264359-m9j3pcj1 34 22 ) ) -RRB- cord-264359-m9j3pcj1 34 23 by by IN cord-264359-m9j3pcj1 34 24 adding add VBG cord-264359-m9j3pcj1 34 25 200 200 CD cord-264359-m9j3pcj1 34 26 pg pg NN cord-264359-m9j3pcj1 34 27 / / SYM cord-264359-m9j3pcj1 34 28 ml ml NN cord-264359-m9j3pcj1 34 29 of of IN cord-264359-m9j3pcj1 34 30 emetine emetine NNP cord-264359-m9j3pcj1 34 31 ( ( -LRB- cord-264359-m9j3pcj1 34 32 Sigma Sigma NNP cord-264359-m9j3pcj1 34 33 ) ) -RRB- cord-264359-m9j3pcj1 34 34 and and CC cord-264359-m9j3pcj1 34 35 a a DT cord-264359-m9j3pcj1 34 36 lOOO looo RB cord-264359-m9j3pcj1 34 37 - - HYPH cord-264359-m9j3pcj1 34 38 fold fold JJ cord-264359-m9j3pcj1 34 39 excess excess NN cord-264359-m9j3pcj1 34 40 of of IN cord-264359-m9j3pcj1 34 41 unlabeled unlabeled JJ cord-264359-m9j3pcj1 34 42 leucine leucine NN cord-264359-m9j3pcj1 34 43 . . . cord-264359-m9j3pcj1 35 1 After after IN cord-264359-m9j3pcj1 35 2 6 6 CD cord-264359-m9j3pcj1 35 3 hr hr NN cord-264359-m9j3pcj1 35 4 of of IN cord-264359-m9j3pcj1 35 5 incubation incubation NN cord-264359-m9j3pcj1 35 6 , , , cord-264359-m9j3pcj1 35 7 the the DT cord-264359-m9j3pcj1 35 8 cells cell NNS cord-264359-m9j3pcj1 35 9 were be VBD cord-264359-m9j3pcj1 35 10 collected collect VBN cord-264359-m9j3pcj1 35 11 by by IN cord-264359-m9j3pcj1 35 12 centrifugation centrifugation NN cord-264359-m9j3pcj1 35 13 and and CC cord-264359-m9j3pcj1 35 14 frozen freeze VBN cord-264359-m9j3pcj1 35 15 to to IN cord-264359-m9j3pcj1 35 16 -70 -70 NN cord-264359-m9j3pcj1 35 17 " " '' cord-264359-m9j3pcj1 35 18 . . . cord-264359-m9j3pcj1 36 1 The the DT cord-264359-m9j3pcj1 36 2 supernatant supernatant NN cord-264359-m9j3pcj1 36 3 was be VBD cord-264359-m9j3pcj1 36 4 again again RB cord-264359-m9j3pcj1 36 5 centrifuged centrifuge VBN cord-264359-m9j3pcj1 36 6 for for IN cord-264359-m9j3pcj1 36 7 90 90 CD cord-264359-m9j3pcj1 36 8 min min NN cord-264359-m9j3pcj1 36 9 at at IN cord-264359-m9j3pcj1 36 10 40,000 40,000 CD cord-264359-m9j3pcj1 36 11 rpm rpm NN cord-264359-m9j3pcj1 36 12 . . . cord-264359-m9j3pcj1 37 1 The the DT cord-264359-m9j3pcj1 37 2 pellet pellet NN cord-264359-m9j3pcj1 37 3 , , , cord-264359-m9j3pcj1 37 4 which which WDT cord-264359-m9j3pcj1 37 5 contained contain VBD cord-264359-m9j3pcj1 37 6 the the DT cord-264359-m9j3pcj1 37 7 extracellular extracellular JJ cord-264359-m9j3pcj1 37 8 virus virus NN cord-264359-m9j3pcj1 37 9 , , , cord-264359-m9j3pcj1 37 10 was be VBD cord-264359-m9j3pcj1 37 11 resuspended resuspend VBN cord-264359-m9j3pcj1 37 12 in in IN cord-264359-m9j3pcj1 37 13 phosphate phosphate NN cord-264359-m9j3pcj1 37 14 buffer buffer NN cord-264359-m9j3pcj1 37 15 . . . cord-264359-m9j3pcj1 38 1 To to TO cord-264359-m9j3pcj1 38 2 prepare prepare VB cord-264359-m9j3pcj1 38 3 cytoplasmic cytoplasmic JJ cord-264359-m9j3pcj1 38 4 extracts extract NNS cord-264359-m9j3pcj1 38 5 , , , cord-264359-m9j3pcj1 38 6 the the DT cord-264359-m9j3pcj1 38 7 thawed thaw VBN cord-264359-m9j3pcj1 38 8 cells cell NNS cord-264359-m9j3pcj1 38 9 were be VBD cord-264359-m9j3pcj1 38 10 resuspended resuspend VBN cord-264359-m9j3pcj1 38 11 for for IN cord-264359-m9j3pcj1 38 12 15 15 CD cord-264359-m9j3pcj1 38 13 min min NN cord-264359-m9j3pcj1 38 14 in in IN cord-264359-m9j3pcj1 38 15 RSB RSB NNP cord-264359-m9j3pcj1 38 16 supplemented supplement VBN cord-264359-m9j3pcj1 38 17 with with IN cord-264359-m9j3pcj1 38 18 excess excess JJ cord-264359-m9j3pcj1 38 19 leucine leucine NN cord-264359-m9j3pcj1 38 20 , , , cord-264359-m9j3pcj1 38 21 disrupted disrupt VBN cord-264359-m9j3pcj1 38 22 in in IN cord-264359-m9j3pcj1 38 23 a a DT cord-264359-m9j3pcj1 38 24 Dounce Dounce NNP cord-264359-m9j3pcj1 38 25 homogenizer homogenizer NN cord-264359-m9j3pcj1 38 26 , , , cord-264359-m9j3pcj1 38 27 and and CC cord-264359-m9j3pcj1 38 28 centrifuged centrifuge VBD cord-264359-m9j3pcj1 38 29 to to TO cord-264359-m9j3pcj1 38 30 remove remove VB cord-264359-m9j3pcj1 38 31 the the DT cord-264359-m9j3pcj1 38 32 nuclei nucleus NNS cord-264359-m9j3pcj1 38 33 . . . cord-264359-m9j3pcj1 39 1 The the DT cord-264359-m9j3pcj1 39 2 extracellular extracellular JJ cord-264359-m9j3pcj1 39 3 virus virus NN cord-264359-m9j3pcj1 39 4 and and CC cord-264359-m9j3pcj1 39 5 120 120 CD cord-264359-m9j3pcj1 39 6 pg pg NN cord-264359-m9j3pcj1 39 7 of of IN cord-264359-m9j3pcj1 39 8 carrier carrier NN cord-264359-m9j3pcj1 39 9 virus virus NN cord-264359-m9j3pcj1 39 10 were be VBD cord-264359-m9j3pcj1 39 11 added add VBN cord-264359-m9j3pcj1 39 12 to to IN cord-264359-m9j3pcj1 39 13 the the DT cord-264359-m9j3pcj1 39 14 cytoplasmic cytoplasmic JJ cord-264359-m9j3pcj1 39 15 extract extract NN cord-264359-m9j3pcj1 39 16 . . . cord-264359-m9j3pcj1 40 1 The the DT cord-264359-m9j3pcj1 40 2 mixture mixture NN cord-264359-m9j3pcj1 40 3 was be VBD cord-264359-m9j3pcj1 40 4 dialyzed dialyze VBN cord-264359-m9j3pcj1 40 5 against against IN cord-264359-m9j3pcj1 40 6 0.01 0.01 CD cord-264359-m9j3pcj1 40 7 M M NNP cord-264359-m9j3pcj1 40 8 phosphate phosphate NN cord-264359-m9j3pcj1 40 9 buffer buffer NN cord-264359-m9j3pcj1 40 10 ( ( -LRB- cord-264359-m9j3pcj1 40 11 pH pH NNP cord-264359-m9j3pcj1 40 12 7.2 7.2 CD cord-264359-m9j3pcj1 40 13 ) ) -RRB- cord-264359-m9j3pcj1 40 14 containing contain VBG cord-264359-m9j3pcj1 40 15 0.5 0.5 CD cord-264359-m9j3pcj1 40 16 % % NN cord-264359-m9j3pcj1 40 17 SDS SDS NNP cord-264359-m9j3pcj1 40 18 and and CC cord-264359-m9j3pcj1 40 19 layered layer VBN cord-264359-m9j3pcj1 40 20 onto onto IN cord-264359-m9j3pcj1 40 21 a a DT cord-264359-m9j3pcj1 40 22 15 15 CD cord-264359-m9j3pcj1 40 23 to to TO cord-264359-m9j3pcj1 40 24 30 30 CD cord-264359-m9j3pcj1 40 25 % % NN cord-264359-m9j3pcj1 40 26 sucrose sucrose NN cord-264359-m9j3pcj1 40 27 gradient gradient NN cord-264359-m9j3pcj1 40 28 . . . cord-264359-m9j3pcj1 41 1 The the DT cord-264359-m9j3pcj1 41 2 sucrose sucrose NN cord-264359-m9j3pcj1 41 3 solutions solution NNS cord-264359-m9j3pcj1 41 4 contained contain VBD cord-264359-m9j3pcj1 41 5 0.01 0.01 CD cord-264359-m9j3pcj1 41 6 M M NNP cord-264359-m9j3pcj1 41 7 phosphate phosphate NN cord-264359-m9j3pcj1 41 8 ( ( -LRB- cord-264359-m9j3pcj1 41 9 pH pH NNP cord-264359-m9j3pcj1 41 10 7.2 7.2 CD cord-264359-m9j3pcj1 41 11 ) ) -RRB- cord-264359-m9j3pcj1 41 12 and and CC cord-264359-m9j3pcj1 41 13 0.5 0.5 CD cord-264359-m9j3pcj1 41 14 % % NN cord-264359-m9j3pcj1 41 15 SDS SDS NNP cord-264359-m9j3pcj1 41 16 . . . cord-264359-m9j3pcj1 42 1 The the DT cord-264359-m9j3pcj1 42 2 virus virus NN cord-264359-m9j3pcj1 42 3 was be VBD cord-264359-m9j3pcj1 42 4 collected collect VBN cord-264359-m9j3pcj1 42 5 as as IN cord-264359-m9j3pcj1 42 6 the the DT cord-264359-m9j3pcj1 42 7 160 160 CD cord-264359-m9j3pcj1 42 8 S S NNP cord-264359-m9j3pcj1 42 9 peak peak NN cord-264359-m9j3pcj1 42 10 after after IN cord-264359-m9j3pcj1 42 11 centrifugation centrifugation NN cord-264359-m9j3pcj1 42 12 for for IN cord-264359-m9j3pcj1 42 13 75 75 CD cord-264359-m9j3pcj1 42 14 min min NN cord-264359-m9j3pcj1 42 15 at at IN cord-264359-m9j3pcj1 42 16 29,000 29,000 CD cord-264359-m9j3pcj1 42 17 rpm rpm NN cord-264359-m9j3pcj1 42 18 . . . cord-264359-m9j3pcj1 43 1 The the DT cord-264359-m9j3pcj1 43 2 " " `` cord-264359-m9j3pcj1 43 3 C c NN cord-264359-m9j3pcj1 43 4 - - HYPH cord-264359-m9j3pcj1 43 5 virus virus NN cord-264359-m9j3pcj1 43 6 used use VBN cord-264359-m9j3pcj1 43 7 for for IN cord-264359-m9j3pcj1 43 8 co co NN cord-264359-m9j3pcj1 43 9 - - NN cord-264359-m9j3pcj1 43 10 electrophoresis electrophoresis JJ cord-264359-m9j3pcj1 43 11 in in IN cord-264359-m9j3pcj1 43 12 these these DT cord-264359-m9j3pcj1 43 13 experiments experiment NNS cord-264359-m9j3pcj1 43 14 was be VBD cord-264359-m9j3pcj1 43 15 separately separately RB cord-264359-m9j3pcj1 43 16 labeled label VBN cord-264359-m9j3pcj1 43 17 with with IN cord-264359-m9j3pcj1 43 18 [ [ -LRB- cord-264359-m9j3pcj1 43 19 ' ' '' cord-264359-m9j3pcj1 43 20 4C]leucine 4c]leucine CD cord-264359-m9j3pcj1 43 21 , , , cord-264359-m9j3pcj1 43 22 purified purify VBN cord-264359-m9j3pcj1 43 23 as as IN cord-264359-m9j3pcj1 43 24 above above RB cord-264359-m9j3pcj1 43 25 , , , cord-264359-m9j3pcj1 43 26 and and CC cord-264359-m9j3pcj1 43 27 added add VBD cord-264359-m9j3pcj1 43 28 immediately immediately RB cord-264359-m9j3pcj1 43 29 before before IN cord-264359-m9j3pcj1 43 30 protein protein NN cord-264359-m9j3pcj1 43 31 extraction extraction NN cord-264359-m9j3pcj1 43 32 . . . cord-264359-m9j3pcj1 44 1 in in IN cord-264359-m9j3pcj1 44 2 pH pH NNP cord-264359-m9j3pcj1 44 3 Gradient Gradient NNP cord-264359-m9j3pcj1 44 4 Electrophoresis Electrophoresis NNP cord-264359-m9j3pcj1 44 5 Figure Figure NNP cord-264359-m9j3pcj1 44 6 1 1 CD cord-264359-m9j3pcj1 44 7 shows show VBZ cord-264359-m9j3pcj1 44 8 the the DT cord-264359-m9j3pcj1 44 9 six six CD cord-264359-m9j3pcj1 44 10 - - HYPH cord-264359-m9j3pcj1 44 11 band band NN cord-264359-m9j3pcj1 44 12 pattern pattern NN cord-264359-m9j3pcj1 44 13 ( ( -LRB- cord-264359-m9j3pcj1 44 14 excluding exclude VBG cord-264359-m9j3pcj1 44 15 VP4 VP4 NNP cord-264359-m9j3pcj1 44 16 ) ) -RRB- cord-264359-m9j3pcj1 44 17 into into IN cord-264359-m9j3pcj1 44 18 which which WDT cord-264359-m9j3pcj1 44 19 the the DT cord-264359-m9j3pcj1 44 20 protein protein NN cord-264359-m9j3pcj1 44 21 of of IN cord-264359-m9j3pcj1 44 22 la la NNP cord-264359-m9j3pcj1 44 23 / / SYM cord-264359-m9j3pcj1 44 24 S3 S3 NNP cord-264359-m9j3pcj1 44 25 poliovirions poliovirion NNS cord-264359-m9j3pcj1 44 26 was be VBD cord-264359-m9j3pcj1 44 27 resolved resolve VBN cord-264359-m9j3pcj1 44 28 by by IN cord-264359-m9j3pcj1 44 29 pH pH NNP cord-264359-m9j3pcj1 44 30 gradient gradient NN cord-264359-m9j3pcj1 44 31 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 44 32 and and CC cord-264359-m9j3pcj1 44 33 the the DT cord-264359-m9j3pcj1 44 34 proposed propose VBN cord-264359-m9j3pcj1 44 35 designation designation NN cord-264359-m9j3pcj1 44 36 of of IN cord-264359-m9j3pcj1 44 37 the the DT cord-264359-m9j3pcj1 44 38 bands band NNS cord-264359-m9j3pcj1 44 39 . . . cord-264359-m9j3pcj1 45 1 Normal normal JJ cord-264359-m9j3pcj1 45 2 SDS sds NN cord-264359-m9j3pcj1 45 3 - - HYPH cord-264359-m9j3pcj1 45 4 PAGE PAGE NNP cord-264359-m9j3pcj1 45 5 of of IN cord-264359-m9j3pcj1 45 6 the the DT cord-264359-m9j3pcj1 45 7 same same JJ cord-264359-m9j3pcj1 45 8 virion virion NN cord-264359-m9j3pcj1 45 9 protein protein NN cord-264359-m9j3pcj1 45 10 ( ( -LRB- cord-264359-m9j3pcj1 45 11 Fig Fig NNP cord-264359-m9j3pcj1 45 12 . . . cord-264359-m9j3pcj1 45 13 2A 2a CD cord-264359-m9j3pcj1 45 14 ) ) -RRB- cord-264359-m9j3pcj1 45 15 yielded yield VBD cord-264359-m9j3pcj1 45 16 only only RB cord-264359-m9j3pcj1 45 17 the the DT cord-264359-m9j3pcj1 45 18 classical classical JJ cord-264359-m9j3pcj1 45 19 VPl-2 vpl-2 NN cord-264359-m9j3pcj1 45 20 - - HYPH cord-264359-m9j3pcj1 45 21 3 3 CD cord-264359-m9j3pcj1 45 22 pattern pattern NN cord-264359-m9j3pcj1 45 23 and and CC cord-264359-m9j3pcj1 45 24 a a DT cord-264359-m9j3pcj1 45 25 trace trace NN cord-264359-m9j3pcj1 45 26 of of IN cord-264359-m9j3pcj1 45 27 VPO VPO NNP cord-264359-m9j3pcj1 45 28 . . . cord-264359-m9j3pcj1 46 1 Samples sample NNS cord-264359-m9j3pcj1 46 2 of of IN cord-264359-m9j3pcj1 46 3 uniformly uniformly RB cord-264359-m9j3pcj1 46 4 14C 14c NN cord-264359-m9j3pcj1 46 5 - - HYPH cord-264359-m9j3pcj1 46 6 labeled label VBN cord-264359-m9j3pcj1 46 7 la la NNP cord-264359-m9j3pcj1 46 8 / / SYM cord-264359-m9j3pcj1 46 9 S3 S3 NNP cord-264359-m9j3pcj1 46 10 protein protein NN cord-264359-m9j3pcj1 46 11 were be VBD cord-264359-m9j3pcj1 46 12 submitted submit VBN cord-264359-m9j3pcj1 46 13 to to IN cord-264359-m9j3pcj1 46 14 either either CC cord-264359-m9j3pcj1 46 15 normal normal JJ cord-264359-m9j3pcj1 46 16 or or CC cord-264359-m9j3pcj1 46 17 pH ph NN cord-264359-m9j3pcj1 46 18 gradient gradient NN cord-264359-m9j3pcj1 46 19 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 46 20 . . . cord-264359-m9j3pcj1 47 1 The the DT cord-264359-m9j3pcj1 47 2 resulting result VBG cord-264359-m9j3pcj1 47 3 radioactivity radioactivity NN cord-264359-m9j3pcj1 47 4 profiles profile NNS cord-264359-m9j3pcj1 47 5 ( ( -LRB- cord-264359-m9j3pcj1 47 6 not not RB cord-264359-m9j3pcj1 47 7 shown show VBN cord-264359-m9j3pcj1 47 8 ) ) -RRB- cord-264359-m9j3pcj1 47 9 confirmed confirm VBD cord-264359-m9j3pcj1 47 10 the the DT cord-264359-m9j3pcj1 47 11 results result NNS cord-264359-m9j3pcj1 47 12 illustrated illustrate VBN cord-264359-m9j3pcj1 47 13 in in IN cord-264359-m9j3pcj1 47 14 Figs Figs NNP cord-264359-m9j3pcj1 47 15 . . . cord-264359-m9j3pcj1 48 1 1 1 CD cord-264359-m9j3pcj1 48 2 and and CC cord-264359-m9j3pcj1 48 3 2A 2a CD cord-264359-m9j3pcj1 49 1 and and CC cord-264359-m9j3pcj1 49 2 were be VBD cord-264359-m9j3pcj1 49 3 in in IN cord-264359-m9j3pcj1 49 4 excellent excellent JJ cord-264359-m9j3pcj1 49 5 agreement agreement NN cord-264359-m9j3pcj1 49 6 with with IN cord-264359-m9j3pcj1 49 7 Frc Frc NNP cord-264359-m9j3pcj1 49 8 . . . cord-264359-m9j3pcj1 50 1 1 1 LS cord-264359-m9j3pcj1 50 2 . . . cord-264359-m9j3pcj1 51 1 pH pH NNP cord-264359-m9j3pcj1 52 1 Gradient Gradient NNP cord-264359-m9j3pcj1 52 2 SDS SDS NNP cord-264359-m9j3pcj1 52 3 - - HYPH cord-264359-m9j3pcj1 52 4 PAGE PAGE NNP cord-264359-m9j3pcj1 52 5 of of IN cord-264359-m9j3pcj1 52 6 virion virion NN cord-264359-m9j3pcj1 52 7 polypeptides polypeptide NNS cord-264359-m9j3pcj1 52 8 ( ( -LRB- cord-264359-m9j3pcj1 52 9 strain strain VBP cord-264359-m9j3pcj1 52 10 la la NNP cord-264359-m9j3pcj1 52 11 / / SYM cord-264359-m9j3pcj1 52 12 S3 S3 NNP cord-264359-m9j3pcj1 52 13 ) ) -RRB- cord-264359-m9j3pcj1 52 14 . . . cord-264359-m9j3pcj1 53 1 Duration duration NN cord-264359-m9j3pcj1 53 2 of of IN cord-264359-m9j3pcj1 53 3 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 53 4 , , , cord-264359-m9j3pcj1 53 5 24 24 CD cord-264359-m9j3pcj1 53 6 hr hr NN cord-264359-m9j3pcj1 53 7 ; ; : cord-264359-m9j3pcj1 53 8 catholyte catholyte NNP cord-264359-m9j3pcj1 53 9 at at IN cord-264359-m9j3pcj1 53 10 pH pH NNP cord-264359-m9j3pcj1 53 11 11.0 11.0 CD cord-264359-m9j3pcj1 53 12 and and CC cord-264359-m9j3pcj1 53 13 anolyte anolyte NNP cord-264359-m9j3pcj1 53 14 at at IN cord-264359-m9j3pcj1 53 15 pH pH NNP cord-264359-m9j3pcj1 53 16 6.5 6.5 CD cord-264359-m9j3pcj1 53 17 . . . cord-264359-m9j3pcj1 54 1 Scale scale NN cord-264359-m9j3pcj1 54 2 , , , cord-264359-m9j3pcj1 54 3 distance distance NN cord-264359-m9j3pcj1 54 4 in in IN cord-264359-m9j3pcj1 54 5 millimeters millimeter NNS cord-264359-m9j3pcj1 54 6 from from IN cord-264359-m9j3pcj1 54 7 cathodic cathodic JJ cord-264359-m9j3pcj1 54 8 end end NN cord-264359-m9j3pcj1 54 9 . . . cord-264359-m9j3pcj1 55 1 Cl Cl NNP cord-264359-m9j3pcj1 55 2 - - HYPH cord-264359-m9j3pcj1 55 3 C6 C6 NNP cord-264359-m9j3pcj1 55 4 , , , cord-264359-m9j3pcj1 55 5 designation designation NN cord-264359-m9j3pcj1 55 6 of of IN cord-264359-m9j3pcj1 55 7 bands band NNS cord-264359-m9j3pcj1 55 8 . . . cord-264359-m9j3pcj1 56 1 Laemmli Laemmli NNP cord-264359-m9j3pcj1 56 2 ( ( -LRB- cord-264359-m9j3pcj1 56 3 1970 1970 CD cord-264359-m9j3pcj1 56 4 ) ) -RRB- cord-264359-m9j3pcj1 56 5 ( ( -LRB- cord-264359-m9j3pcj1 56 6 3 3 CD cord-264359-m9j3pcj1 56 7 mA mA NNP cord-264359-m9j3pcj1 56 8 / / SYM cord-264359-m9j3pcj1 56 9 gel gel NN cord-264359-m9j3pcj1 56 10 , , , cord-264359-m9j3pcj1 56 11 5.5 5.5 CD cord-264359-m9j3pcj1 56 12 hr hr NN cord-264359-m9j3pcj1 56 13 ) ) -RRB- cord-264359-m9j3pcj1 56 14 ; ; : cord-264359-m9j3pcj1 56 15 the the DT cord-264359-m9j3pcj1 56 16 acrylamide acrylamide NN cord-264359-m9j3pcj1 56 17 and and CC cord-264359-m9j3pcj1 56 18 bisacrylamide bisacrylamide NNP cord-264359-m9j3pcj1 56 19 concentrations concentration NNS cord-264359-m9j3pcj1 56 20 in in IN cord-264359-m9j3pcj1 56 21 the the DT cord-264359-m9j3pcj1 56 22 separation separation NN cord-264359-m9j3pcj1 56 23 gel gel NN cord-264359-m9j3pcj1 56 24 were be VBD cord-264359-m9j3pcj1 56 25 increased increase VBN cord-264359-m9j3pcj1 56 26 to to IN cord-264359-m9j3pcj1 56 27 12.5 12.5 CD cord-264359-m9j3pcj1 56 28 and and CC cord-264359-m9j3pcj1 56 29 0.3331 0.3331 CD cord-264359-m9j3pcj1 56 30 , , , cord-264359-m9j3pcj1 56 31 respectively respectively RB cord-264359-m9j3pcj1 56 32 . . . cord-264359-m9j3pcj1 57 1 Scale scale NN cord-264359-m9j3pcj1 57 2 , , , cord-264359-m9j3pcj1 57 3 distance distance NN cord-264359-m9j3pcj1 57 4 in in IN cord-264359-m9j3pcj1 57 5 millimeters millimeter NNS cord-264359-m9j3pcj1 57 6 from from IN cord-264359-m9j3pcj1 57 7 cathodic cathodic JJ cord-264359-m9j3pcj1 57 8 end end NN cord-264359-m9j3pcj1 57 9 . . . cord-264359-m9j3pcj1 58 1 WOUTERS WOUTERS NNP cord-264359-m9j3pcj1 58 2 . . . cord-264359-m9j3pcj1 59 1 AND and CC cord-264359-m9j3pcj1 59 2 BOEYE BOEYE NNP cord-264359-m9j3pcj1 59 3 the the DT cord-264359-m9j3pcj1 59 4 densitometric densitometric JJ cord-264359-m9j3pcj1 59 5 scans scan NNS cord-264359-m9j3pcj1 59 6 of of IN cord-264359-m9j3pcj1 59 7 duplicate duplicate JJ cord-264359-m9j3pcj1 59 8 gels gel NNS cord-264359-m9j3pcj1 59 9 stained stain VBN cord-264359-m9j3pcj1 59 10 with with IN cord-264359-m9j3pcj1 59 11 Coomassie Coomassie NNP cord-264359-m9j3pcj1 59 12 blue blue NN cord-264359-m9j3pcj1 59 13 . . . cord-264359-m9j3pcj1 60 1 As as IN cord-264359-m9j3pcj1 60 2 illustrated illustrate VBN cord-264359-m9j3pcj1 60 3 in in IN cord-264359-m9j3pcj1 60 4 Fig Fig NNP cord-264359-m9j3pcj1 60 5 . . . cord-264359-m9j3pcj1 60 6 2B 2b CD cord-264359-m9j3pcj1 60 7 , , , cord-264359-m9j3pcj1 60 8 discontinuous discontinuous JJ cord-264359-m9j3pcj1 60 9 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 60 10 according accord VBG cord-264359-m9j3pcj1 60 11 to to IN cord-264359-m9j3pcj1 60 12 Laemmli Laemmli NNP cord-264359-m9j3pcj1 60 13 ( ( -LRB- cord-264359-m9j3pcj1 60 14 1970 1970 CD cord-264359-m9j3pcj1 60 15 ) ) -RRB- cord-264359-m9j3pcj1 60 16 failed fail VBD cord-264359-m9j3pcj1 60 17 to to TO cord-264359-m9j3pcj1 60 18 yield yield VB cord-264359-m9j3pcj1 60 19 the the DT cord-264359-m9j3pcj1 60 20 fine fine JJ cord-264359-m9j3pcj1 60 21 resolution resolution NN cord-264359-m9j3pcj1 60 22 which which WDT cord-264359-m9j3pcj1 60 23 was be VBD cord-264359-m9j3pcj1 60 24 achieved achieve VBN cord-264359-m9j3pcj1 60 25 by by IN cord-264359-m9j3pcj1 60 26 pH pH NNP cord-264359-m9j3pcj1 60 27 gradient gradient NN cord-264359-m9j3pcj1 60 28 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 60 29 . . . cord-264359-m9j3pcj1 61 1 Our -PRON- PRP$ cord-264359-m9j3pcj1 61 2 usual usual JJ cord-264359-m9j3pcj1 61 3 purification purification NN cord-264359-m9j3pcj1 61 4 procedure procedure NN cord-264359-m9j3pcj1 61 5 of of IN cord-264359-m9j3pcj1 61 6 la la NNP cord-264359-m9j3pcj1 61 7 / / SYM cord-264359-m9j3pcj1 61 8 S3 S3 NNP cord-264359-m9j3pcj1 61 9 virus virus NN cord-264359-m9j3pcj1 61 10 involves involve VBZ cord-264359-m9j3pcj1 61 11 the the DT cord-264359-m9j3pcj1 61 12 collection collection NN cord-264359-m9j3pcj1 61 13 of of IN cord-264359-m9j3pcj1 61 14 extracellular extracellular JJ cord-264359-m9j3pcj1 61 15 virus virus NN cord-264359-m9j3pcj1 61 16 at at IN cord-264359-m9j3pcj1 61 17 about about RB cord-264359-m9j3pcj1 61 18 28 28 CD cord-264359-m9j3pcj1 61 19 hr hr NN cord-264359-m9j3pcj1 61 20 postinfection postinfection NN cord-264359-m9j3pcj1 61 21 , , , cord-264359-m9j3pcj1 61 22 concentration concentration NN cord-264359-m9j3pcj1 61 23 by by IN cord-264359-m9j3pcj1 61 24 flash flash NN cord-264359-m9j3pcj1 61 25 evaporation evaporation NN cord-264359-m9j3pcj1 61 26 , , , cord-264359-m9j3pcj1 61 27 two two CD cord-264359-m9j3pcj1 61 28 rounds round NNS cord-264359-m9j3pcj1 61 29 of of IN cord-264359-m9j3pcj1 61 30 centrifugation centrifugation NN cord-264359-m9j3pcj1 61 31 , , , cord-264359-m9j3pcj1 61 32 a a DT cord-264359-m9j3pcj1 61 33 butanol butanol NN cord-264359-m9j3pcj1 61 34 extraction extraction NN cord-264359-m9j3pcj1 61 35 , , , cord-264359-m9j3pcj1 61 36 and and CC cord-264359-m9j3pcj1 61 37 ECTEOLA ecteola NN cord-264359-m9j3pcj1 61 38 -cellulose -cellulose WDT cord-264359-m9j3pcj1 61 39 chromatography chromatography NN cord-264359-m9j3pcj1 61 40 ( ( -LRB- cord-264359-m9j3pcj1 61 41 Boeye Boeye NNP cord-264359-m9j3pcj1 61 42 , , , cord-264359-m9j3pcj1 61 43 1965 1965 CD cord-264359-m9j3pcj1 61 44 ) ) -RRB- cord-264359-m9j3pcj1 61 45 . . . cord-264359-m9j3pcj1 62 1 This this DT cord-264359-m9j3pcj1 62 2 procedure procedure NN cord-264359-m9j3pcj1 62 3 was be VBD cord-264359-m9j3pcj1 62 4 modified modify VBN cord-264359-m9j3pcj1 62 5 in in IN cord-264359-m9j3pcj1 62 6 three three CD cord-264359-m9j3pcj1 62 7 different different JJ cord-264359-m9j3pcj1 62 8 ways way NNS cord-264359-m9j3pcj1 62 9 in in IN cord-264359-m9j3pcj1 62 10 separate separate JJ cord-264359-m9j3pcj1 62 11 experiments experiment NNS cord-264359-m9j3pcj1 62 12 : : : cord-264359-m9j3pcj1 62 13 ( ( -LRB- cord-264359-m9j3pcj1 62 14 i i NN cord-264359-m9j3pcj1 62 15 ) ) -RRB- cord-264359-m9j3pcj1 63 1 The the DT cord-264359-m9j3pcj1 63 2 virus virus NN cord-264359-m9j3pcj1 63 3 was be VBD cord-264359-m9j3pcj1 63 4 centrifuged centrifuge VBN cord-264359-m9j3pcj1 63 5 in in IN cord-264359-m9j3pcj1 63 6 a a DT cord-264359-m9j3pcj1 63 7 15 15 CD cord-264359-m9j3pcj1 63 8 to to TO cord-264359-m9j3pcj1 63 9 30 30 CD cord-264359-m9j3pcj1 63 10 % % NN cord-264359-m9j3pcj1 63 11 sucrose sucrose NN cord-264359-m9j3pcj1 63 12 gradient gradient NN cord-264359-m9j3pcj1 63 13 and and CC cord-264359-m9j3pcj1 63 14 collected collect VBD cord-264359-m9j3pcj1 63 15 from from IN cord-264359-m9j3pcj1 63 16 the the DT cord-264359-m9j3pcj1 63 17 160 160 CD cord-264359-m9j3pcj1 63 18 S S NNP cord-264359-m9j3pcj1 63 19 peak peak NN cord-264359-m9j3pcj1 63 20 as as IN cord-264359-m9j3pcj1 63 21 an an DT cord-264359-m9j3pcj1 63 22 additional additional JJ cord-264359-m9j3pcj1 63 23 purification purification NN cord-264359-m9j3pcj1 63 24 step step NN cord-264359-m9j3pcj1 63 25 ; ; : cord-264359-m9j3pcj1 63 26 or or CC cord-264359-m9j3pcj1 63 27 ( ( -LRB- cord-264359-m9j3pcj1 63 28 ii ii NN cord-264359-m9j3pcj1 63 29 ) ) -RRB- cord-264359-m9j3pcj1 63 30 intracellular intracellular JJ cord-264359-m9j3pcj1 64 1 ( ( -LRB- cord-264359-m9j3pcj1 64 2 instead instead RB cord-264359-m9j3pcj1 64 3 of of IN cord-264359-m9j3pcj1 64 4 extracellular extracellular JJ cord-264359-m9j3pcj1 64 5 ) ) -RRB- cord-264359-m9j3pcj1 64 6 virus virus NN cord-264359-m9j3pcj1 64 7 was be VBD cord-264359-m9j3pcj1 64 8 harvested harvest VBN cord-264359-m9j3pcj1 64 9 by by IN cord-264359-m9j3pcj1 64 10 freezing freeze VBG cord-264359-m9j3pcj1 64 11 and and CC cord-264359-m9j3pcj1 64 12 thawing thawing NN cord-264359-m9j3pcj1 64 13 of of IN cord-264359-m9j3pcj1 64 14 the the DT cord-264359-m9j3pcj1 64 15 cells cell NNS cord-264359-m9j3pcj1 64 16 , , , cord-264359-m9j3pcj1 64 17 the the DT cord-264359-m9j3pcj1 64 18 other other JJ cord-264359-m9j3pcj1 64 19 steps step NNS cord-264359-m9j3pcj1 64 20 of of IN cord-264359-m9j3pcj1 64 21 the the DT cord-264359-m9j3pcj1 64 22 procedure procedure NN cord-264359-m9j3pcj1 64 23 remaining remain VBG cord-264359-m9j3pcj1 64 24 unchanged unchanged JJ cord-264359-m9j3pcj1 64 25 ; ; : cord-264359-m9j3pcj1 64 26 or or CC cord-264359-m9j3pcj1 64 27 ( ( -LRB- cord-264359-m9j3pcj1 64 28 iii iii NN cord-264359-m9j3pcj1 64 29 ) ) -RRB- cord-264359-m9j3pcj1 65 1 the the DT cord-264359-m9j3pcj1 65 2 virus virus NN cord-264359-m9j3pcj1 65 3 was be VBD cord-264359-m9j3pcj1 65 4 harvested harvest VBN cord-264359-m9j3pcj1 65 5 prematurely prematurely RB cord-264359-m9j3pcj1 65 6 ( ( -LRB- cord-264359-m9j3pcj1 65 7 9 9 CD cord-264359-m9j3pcj1 65 8 hr hr NN cord-264359-m9j3pcj1 65 9 p.i p.i NNP cord-264359-m9j3pcj1 65 10 . . NNP cord-264359-m9j3pcj1 65 11 ) ) -RRB- cord-264359-m9j3pcj1 65 12 by by IN cord-264359-m9j3pcj1 65 13 homogenizing homogenize VBG cord-264359-m9j3pcj1 65 14 the the DT cord-264359-m9j3pcj1 65 15 cells cell NNS cord-264359-m9j3pcj1 65 16 and and CC cord-264359-m9j3pcj1 65 17 purified purify VBN cord-264359-m9j3pcj1 65 18 by by IN cord-264359-m9j3pcj1 65 19 sucrose sucrose NN cord-264359-m9j3pcj1 65 20 gradient gradient NN cord-264359-m9j3pcj1 65 21 centrifugation centrifugation NN cord-264359-m9j3pcj1 65 22 in in IN cord-264359-m9j3pcj1 65 23 the the DT cord-264359-m9j3pcj1 65 24 presence presence NN cord-264359-m9j3pcj1 65 25 of of IN cord-264359-m9j3pcj1 65 26 0.5 0.5 CD cord-264359-m9j3pcj1 65 27 % % NN cord-264359-m9j3pcj1 65 28 SDS SDS NNP cord-264359-m9j3pcj1 65 29 ( ( -LRB- cord-264359-m9j3pcj1 65 30 for for IN cord-264359-m9j3pcj1 65 31 a a DT cord-264359-m9j3pcj1 65 32 detailed detailed JJ cord-264359-m9j3pcj1 65 33 description description NN cord-264359-m9j3pcj1 65 34 of of IN cord-264359-m9j3pcj1 65 35 the the DT cord-264359-m9j3pcj1 65 36 procedure procedure NN cord-264359-m9j3pcj1 65 37 , , , cord-264359-m9j3pcj1 65 38 see see VBP cord-264359-m9j3pcj1 65 39 under under IN cord-264359-m9j3pcj1 65 40 Materials material NNS cord-264359-m9j3pcj1 65 41 and and CC cord-264359-m9j3pcj1 65 42 Methods Methods NNPS cord-264359-m9j3pcj1 65 43 , , , cord-264359-m9j3pcj1 65 44 Incorporation Incorporation NNP cord-264359-m9j3pcj1 65 45 studies study NNS cord-264359-m9j3pcj1 65 46 using use VBG cord-264359-m9j3pcj1 65 47 emetine emetine NNP cord-264359-m9j3pcj1 65 48 ) ) -RRB- cord-264359-m9j3pcj1 65 49 . . . cord-264359-m9j3pcj1 66 1 None none NN cord-264359-m9j3pcj1 66 2 of of IN cord-264359-m9j3pcj1 66 3 the the DT cord-264359-m9j3pcj1 66 4 modifications modification NNS cord-264359-m9j3pcj1 66 5 to to IN cord-264359-m9j3pcj1 66 6 the the DT cord-264359-m9j3pcj1 66 7 usual usual JJ cord-264359-m9j3pcj1 66 8 virus virus NN cord-264359-m9j3pcj1 66 9 purification purification NN cord-264359-m9j3pcj1 66 10 procedure procedure NN cord-264359-m9j3pcj1 66 11 altered alter VBD cord-264359-m9j3pcj1 66 12 the the DT cord-264359-m9j3pcj1 66 13 results result NNS cord-264359-m9j3pcj1 66 14 of of IN cord-264359-m9j3pcj1 66 15 normal normal JJ cord-264359-m9j3pcj1 66 16 or or CC cord-264359-m9j3pcj1 66 17 pH ph NN cord-264359-m9j3pcj1 66 18 gradient gradient NN cord-264359-m9j3pcj1 66 19 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 66 20 of of IN cord-264359-m9j3pcj1 66 21 the the DT cord-264359-m9j3pcj1 66 22 viral viral JJ cord-264359-m9j3pcj1 66 23 capsid capsid NN cord-264359-m9j3pcj1 66 24 polypeptides polypeptide NNS cord-264359-m9j3pcj1 66 25 . . . cord-264359-m9j3pcj1 67 1 The the DT cord-264359-m9j3pcj1 67 2 protein protein NN cord-264359-m9j3pcj1 67 3 extraction extraction NN cord-264359-m9j3pcj1 67 4 procedure procedure NN cord-264359-m9j3pcj1 67 5 was be VBD cord-264359-m9j3pcj1 67 6 also also RB cord-264359-m9j3pcj1 67 7 modified modify VBN cord-264359-m9j3pcj1 67 8 in in IN cord-264359-m9j3pcj1 67 9 two two CD cord-264359-m9j3pcj1 67 10 different different JJ cord-264359-m9j3pcj1 67 11 ways way NNS cord-264359-m9j3pcj1 67 12 . . . cord-264359-m9j3pcj1 68 1 Instead instead RB cord-264359-m9j3pcj1 68 2 of of IN cord-264359-m9j3pcj1 68 3 the the DT cord-264359-m9j3pcj1 68 4 procedure procedure NN cord-264359-m9j3pcj1 68 5 described describe VBN cord-264359-m9j3pcj1 68 6 under under IN cord-264359-m9j3pcj1 68 7 Materials material NNS cord-264359-m9j3pcj1 68 8 and and CC cord-264359-m9j3pcj1 68 9 Methods Methods NNPS cord-264359-m9j3pcj1 68 10 , , , cord-264359-m9j3pcj1 68 11 the the DT cord-264359-m9j3pcj1 68 12 virus virus NN cord-264359-m9j3pcj1 68 13 suspension suspension NN cord-264359-m9j3pcj1 68 14 was be VBD cord-264359-m9j3pcj1 68 15 either either RB cord-264359-m9j3pcj1 69 1 ( ( -LRB- cord-264359-m9j3pcj1 69 2 i i NN cord-264359-m9j3pcj1 69 3 ) ) -RRB- cord-264359-m9j3pcj1 69 4 heated heat VBD cord-264359-m9j3pcj1 69 5 for for IN cord-264359-m9j3pcj1 69 6 5 5 CD cord-264359-m9j3pcj1 69 7 min min NN cord-264359-m9j3pcj1 69 8 at at IN cord-264359-m9j3pcj1 69 9 100 100 CD cord-264359-m9j3pcj1 69 10 ' ' '' cord-264359-m9j3pcj1 69 11 in in IN cord-264359-m9j3pcj1 69 12 the the DT cord-264359-m9j3pcj1 69 13 presence presence NN cord-264359-m9j3pcj1 69 14 of of IN cord-264359-m9j3pcj1 69 15 1 1 CD cord-264359-m9j3pcj1 69 16 % % NN cord-264359-m9j3pcj1 69 17 SDS SDS NNP cord-264359-m9j3pcj1 69 18 and and CC cord-264359-m9j3pcj1 69 19 1 1 CD cord-264359-m9j3pcj1 69 20 % % NN cord-264359-m9j3pcj1 69 21 mercaptoethanol mercaptoethanol NNS cord-264359-m9j3pcj1 69 22 or or CC cord-264359-m9j3pcj1 69 23 ( ( -LRB- cord-264359-m9j3pcj1 69 24 ii ii NN cord-264359-m9j3pcj1 69 25 ) ) -RRB- cord-264359-m9j3pcj1 69 26 dialyzed dialyze VBN cord-264359-m9j3pcj1 69 27 twice twice RB cord-264359-m9j3pcj1 69 28 for for IN cord-264359-m9j3pcj1 69 29 24 24 CD cord-264359-m9j3pcj1 69 30 hr hr NN cord-264359-m9j3pcj1 69 31 at at IN cord-264359-m9j3pcj1 69 32 37 37 CD cord-264359-m9j3pcj1 69 33 " " `` cord-264359-m9j3pcj1 69 34 against against IN cord-264359-m9j3pcj1 69 35 100 100 CD cord-264359-m9j3pcj1 69 36 vol vol NN cord-264359-m9j3pcj1 69 37 of of IN cord-264359-m9j3pcj1 69 38 0.01 0.01 CD cord-264359-m9j3pcj1 69 39 M m NN cord-264359-m9j3pcj1 69 40 phosphate phosphate NN cord-264359-m9j3pcj1 69 41 buffer buffer NN cord-264359-m9j3pcj1 69 42 ( ( -LRB- cord-264359-m9j3pcj1 69 43 pH pH NNP cord-264359-m9j3pcj1 69 44 7.2 7.2 CD cord-264359-m9j3pcj1 69 45 ) ) -RRB- cord-264359-m9j3pcj1 69 46 containing contain VBG cord-264359-m9j3pcj1 69 47 0.1 0.1 CD cord-264359-m9j3pcj1 69 48 % % NN cord-264359-m9j3pcj1 69 49 SDS SDS NNP cord-264359-m9j3pcj1 69 50 and and CC cord-264359-m9j3pcj1 69 51 5 5 CD cord-264359-m9j3pcj1 69 52 M m NN cord-264359-m9j3pcj1 69 53 urea urea NN cord-264359-m9j3pcj1 69 54 in in IN cord-264359-m9j3pcj1 69 55 the the DT cord-264359-m9j3pcj1 69 56 first first JJ cord-264359-m9j3pcj1 69 57 and and CC cord-264359-m9j3pcj1 69 58 0.1 0.1 CD cord-264359-m9j3pcj1 69 59 % % NN cord-264359-m9j3pcj1 69 60 SDS sds NN cord-264359-m9j3pcj1 69 61 and and CC cord-264359-m9j3pcj1 69 62 0.1 0.1 CD cord-264359-m9j3pcj1 69 63 % % NN cord-264359-m9j3pcj1 69 64 mercaptoethanol mercaptoethanol NNS cord-264359-m9j3pcj1 69 65 in in IN cord-264359-m9j3pcj1 69 66 the the DT cord-264359-m9j3pcj1 69 67 second second JJ cord-264359-m9j3pcj1 69 68 dialysis dialysis NN cord-264359-m9j3pcj1 69 69 ( ( -LRB- cord-264359-m9j3pcj1 69 70 after after IN cord-264359-m9j3pcj1 69 71 Vanden Vanden NNP cord-264359-m9j3pcj1 69 72 Berghe Berghe NNP cord-264359-m9j3pcj1 69 73 and and CC cord-264359-m9j3pcj1 69 74 Boeye Boeye NNP cord-264359-m9j3pcj1 69 75 , , , cord-264359-m9j3pcj1 69 76 1972 1972 CD cord-264359-m9j3pcj1 69 77 ) ) -RRB- cord-264359-m9j3pcj1 69 78 . . . cord-264359-m9j3pcj1 70 1 Again again RB cord-264359-m9j3pcj1 70 2 , , , cord-264359-m9j3pcj1 70 3 the the DT cord-264359-m9j3pcj1 70 4 results result NNS cord-264359-m9j3pcj1 70 5 of of IN cord-264359-m9j3pcj1 70 6 both both CC cord-264359-m9j3pcj1 70 7 normal normal JJ cord-264359-m9j3pcj1 70 8 and and CC cord-264359-m9j3pcj1 70 9 pH ph NN cord-264359-m9j3pcj1 70 10 gradient gradient NN cord-264359-m9j3pcj1 70 11 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 70 12 of of IN cord-264359-m9j3pcj1 70 13 the the DT cord-264359-m9j3pcj1 70 14 viral viral JJ cord-264359-m9j3pcj1 70 15 protein protein NN cord-264359-m9j3pcj1 70 16 were be VBD cord-264359-m9j3pcj1 70 17 unchanged unchanged JJ cord-264359-m9j3pcj1 70 18 . . . cord-264359-m9j3pcj1 71 1 In in IN cord-264359-m9j3pcj1 71 2 the the DT cord-264359-m9j3pcj1 71 3 last last JJ cord-264359-m9j3pcj1 71 4 step step NN cord-264359-m9j3pcj1 71 5 of of IN cord-264359-m9j3pcj1 71 6 our -PRON- PRP$ cord-264359-m9j3pcj1 71 7 usual usual JJ cord-264359-m9j3pcj1 71 8 purification purification NN cord-264359-m9j3pcj1 71 9 procedure procedure NN cord-264359-m9j3pcj1 71 10 , , , cord-264359-m9j3pcj1 71 11 the the DT cord-264359-m9j3pcj1 71 12 virus virus NN cord-264359-m9j3pcj1 71 13 is be VBZ cord-264359-m9j3pcj1 71 14 eluted elute VBN cord-264359-m9j3pcj1 71 15 from from IN cord-264359-m9j3pcj1 71 16 EC EC NNP cord-264359-m9j3pcj1 71 17 - - HYPH cord-264359-m9j3pcj1 71 18 TEOLA TEOLA NNP cord-264359-m9j3pcj1 71 19 - - HYPH cord-264359-m9j3pcj1 71 20 cellulose cellulose NN cord-264359-m9j3pcj1 71 21 using use VBG cord-264359-m9j3pcj1 71 22 0.02 0.02 CD cord-264359-m9j3pcj1 71 23 M M NNP cord-264359-m9j3pcj1 71 24 phosphate phosphate NN cord-264359-m9j3pcj1 71 25 buffer buffer NN cord-264359-m9j3pcj1 71 26 of of IN cord-264359-m9j3pcj1 71 27 pH pH NNP cord-264359-m9j3pcj1 71 28 7.2 7.2 CD cord-264359-m9j3pcj1 71 29 . . . cord-264359-m9j3pcj1 72 1 Reelution reelution NN cord-264359-m9j3pcj1 72 2 of of IN cord-264359-m9j3pcj1 72 3 the the DT cord-264359-m9j3pcj1 72 4 column column NN cord-264359-m9j3pcj1 72 5 using use VBG cord-264359-m9j3pcj1 72 6 the the DT cord-264359-m9j3pcj1 72 7 same same JJ cord-264359-m9j3pcj1 72 8 buffer buffer NN cord-264359-m9j3pcj1 72 9 supplemented supplement VBN cord-264359-m9j3pcj1 72 10 with with IN cord-264359-m9j3pcj1 72 11 0.5 0.5 CD cord-264359-m9j3pcj1 72 12 M M NNP cord-264359-m9j3pcj1 72 13 NaCl NaCl NNP cord-264359-m9j3pcj1 72 14 yielded yield VBD cord-264359-m9j3pcj1 72 15 a a DT cord-264359-m9j3pcj1 72 16 mixture mixture NN cord-264359-m9j3pcj1 72 17 of of IN cord-264359-m9j3pcj1 72 18 virions virion NNS cord-264359-m9j3pcj1 72 19 and and CC cord-264359-m9j3pcj1 72 20 empty empty JJ cord-264359-m9j3pcj1 72 21 particles particle NNS cord-264359-m9j3pcj1 72 22 from from IN cord-264359-m9j3pcj1 72 23 which which WDT cord-264359-m9j3pcj1 72 24 the the DT cord-264359-m9j3pcj1 72 25 latter latter JJ cord-264359-m9j3pcj1 72 26 were be VBD cord-264359-m9j3pcj1 72 27 purified purify VBN cord-264359-m9j3pcj1 72 28 as as IN cord-264359-m9j3pcj1 72 29 the the DT cord-264359-m9j3pcj1 72 30 80 80 CD cord-264359-m9j3pcj1 72 31 S S NNP cord-264359-m9j3pcj1 72 32 peak peak NN cord-264359-m9j3pcj1 72 33 in in IN cord-264359-m9j3pcj1 72 34 a a DT cord-264359-m9j3pcj1 72 35 15 15 CD cord-264359-m9j3pcj1 72 36 to to TO cord-264359-m9j3pcj1 72 37 30 30 CD cord-264359-m9j3pcj1 72 38 % % NN cord-264359-m9j3pcj1 72 39 sucrose sucrose NN cord-264359-m9j3pcj1 72 40 gradient gradient NN cord-264359-m9j3pcj1 72 41 . . . cord-264359-m9j3pcj1 73 1 These these DT cord-264359-m9j3pcj1 73 2 empty empty JJ cord-264359-m9j3pcj1 73 3 particles particle NNS cord-264359-m9j3pcj1 73 4 were be VBD cord-264359-m9j3pcj1 73 5 found find VBN cord-264359-m9j3pcj1 73 6 by by IN cord-264359-m9j3pcj1 73 7 the the DT cord-264359-m9j3pcj1 73 8 normal normal JJ cord-264359-m9j3pcj1 73 9 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 73 10 to to TO cord-264359-m9j3pcj1 73 11 contain contain VB cord-264359-m9j3pcj1 73 12 VPO VPO NNP cord-264359-m9j3pcj1 73 13 , , , cord-264359-m9j3pcj1 73 14 VPl VPl NNP cord-264359-m9j3pcj1 73 15 , , , cord-264359-m9j3pcj1 73 16 and and CC cord-264359-m9j3pcj1 73 17 VP3 vp3 NN cord-264359-m9j3pcj1 73 18 but but CC cord-264359-m9j3pcj1 73 19 no no DT cord-264359-m9j3pcj1 73 20 VP2 vp2 NN cord-264359-m9j3pcj1 73 21 or or CC cord-264359-m9j3pcj1 73 22 VP4 VP4 NNP cord-264359-m9j3pcj1 73 23 , , , cord-264359-m9j3pcj1 73 24 which which WDT cord-264359-m9j3pcj1 73 25 is be VBZ cord-264359-m9j3pcj1 73 26 in in IN cord-264359-m9j3pcj1 73 27 agreement agreement NN cord-264359-m9j3pcj1 73 28 with with IN cord-264359-m9j3pcj1 73 29 the the DT cord-264359-m9j3pcj1 73 30 known know VBN cord-264359-m9j3pcj1 73 31 composition composition NN cord-264359-m9j3pcj1 73 32 of of IN cord-264359-m9j3pcj1 73 33 procapsids procapsid NNS cord-264359-m9j3pcj1 73 34 ( ( -LRB- cord-264359-m9j3pcj1 73 35 Maize1 Maize1 NNP cord-264359-m9j3pcj1 73 36 et et NNP cord-264359-m9j3pcj1 73 37 al al NNP cord-264359-m9j3pcj1 73 38 . . NNP cord-264359-m9j3pcj1 73 39 , , , cord-264359-m9j3pcj1 73 40 1967 1967 CD cord-264359-m9j3pcj1 73 41 ; ; : cord-264359-m9j3pcj1 73 42 Phillips Phillips NNP cord-264359-m9j3pcj1 73 43 and and CC cord-264359-m9j3pcj1 73 44 Fennell Fennell NNP cord-264359-m9j3pcj1 73 45 , , , cord-264359-m9j3pcj1 73 46 1973 1973 CD cord-264359-m9j3pcj1 73 47 ) ) -RRB- cord-264359-m9j3pcj1 73 48 . . . cord-264359-m9j3pcj1 74 1 The the DT cord-264359-m9j3pcj1 74 2 components component NNS cord-264359-m9j3pcj1 74 3 resolved resolve VBN cord-264359-m9j3pcj1 74 4 by by IN cord-264359-m9j3pcj1 74 5 pH pH NNP cord-264359-m9j3pcj1 74 6 gradient gradient NN cord-264359-m9j3pcj1 74 7 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 74 8 were be VBD cord-264359-m9j3pcj1 74 9 VPO VPO NNP cord-264359-m9j3pcj1 74 10 , , , cord-264359-m9j3pcj1 74 11 Cl Cl NNP cord-264359-m9j3pcj1 74 12 , , , cord-264359-m9j3pcj1 74 13 C2 C2 NNP cord-264359-m9j3pcj1 74 14 , , , cord-264359-m9j3pcj1 74 15 and and CC cord-264359-m9j3pcj1 74 16 a a DT cord-264359-m9j3pcj1 74 17 C5-C6 c5-c6 NN cord-264359-m9j3pcj1 74 18 group group NN cord-264359-m9j3pcj1 74 19 ( ( -LRB- cord-264359-m9j3pcj1 74 20 Fig Fig NNP cord-264359-m9j3pcj1 74 21 . . . cord-264359-m9j3pcj1 74 22 3 3 CD cord-264359-m9j3pcj1 74 23 ) ) -RRB- cord-264359-m9j3pcj1 74 24 . . . cord-264359-m9j3pcj1 75 1 Thus thus RB cord-264359-m9j3pcj1 75 2 , , , cord-264359-m9j3pcj1 75 3 the the DT cord-264359-m9j3pcj1 75 4 protein protein NN cord-264359-m9j3pcj1 75 5 of of IN cord-264359-m9j3pcj1 75 6 procapsids procapsid NNS cord-264359-m9j3pcj1 75 7 could could MD cord-264359-m9j3pcj1 75 8 be be VB cord-264359-m9j3pcj1 75 9 further further RB cord-264359-m9j3pcj1 75 10 resolved resolve VBN cord-264359-m9j3pcj1 75 11 by by IN cord-264359-m9j3pcj1 75 12 pH pH NNP cord-264359-m9j3pcj1 75 13 gradient gradient NN cord-264359-m9j3pcj1 75 14 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 75 15 , , , cord-264359-m9j3pcj1 75 16 just just RB cord-264359-m9j3pcj1 75 17 like like IN cord-264359-m9j3pcj1 75 18 that that DT cord-264359-m9j3pcj1 75 19 of of IN cord-264359-m9j3pcj1 75 20 mature mature JJ cord-264359-m9j3pcj1 75 21 virions virion NNS cord-264359-m9j3pcj1 75 22 . . . cord-264359-m9j3pcj1 76 1 Tritiated Tritiated NNP cord-264359-m9j3pcj1 76 2 MEF-1 MEF-1 NNP cord-264359-m9j3pcj1 77 1 ( ( -LRB- cord-264359-m9j3pcj1 77 2 type type NN cord-264359-m9j3pcj1 77 3 2 2 CD cord-264359-m9j3pcj1 77 4 ) ) -RRB- cord-264359-m9j3pcj1 77 5 virion virion NN cord-264359-m9j3pcj1 77 6 protein protein NN cord-264359-m9j3pcj1 77 7 was be VBD cord-264359-m9j3pcj1 77 8 co co JJ cord-264359-m9j3pcj1 77 9 - - JJ cord-264359-m9j3pcj1 77 10 electrophoresed electrophoresed JJ cord-264359-m9j3pcj1 77 11 in in IN cord-264359-m9j3pcj1 77 12 the the DT cord-264359-m9j3pcj1 77 13 pH pH NNP cord-264359-m9j3pcj1 77 14 gradient gradient NN cord-264359-m9j3pcj1 77 15 technique technique NN cord-264359-m9j3pcj1 77 16 with with IN cord-264359-m9j3pcj1 77 17 " " `` cord-264359-m9j3pcj1 77 18 C C NNP cord-264359-m9j3pcj1 77 19 - - HYPH cord-264359-m9j3pcj1 77 20 labeled label VBN cord-264359-m9j3pcj1 77 21 la la NNP cord-264359-m9j3pcj1 77 22 / / SYM cord-264359-m9j3pcj1 77 23 S3 S3 NNP cord-264359-m9j3pcj1 77 24 ( ( -LRB- cord-264359-m9j3pcj1 77 25 type type NN cord-264359-m9j3pcj1 77 26 1 1 CD cord-264359-m9j3pcj1 77 27 ) ) -RRB- cord-264359-m9j3pcj1 77 28 protein protein NN cord-264359-m9j3pcj1 77 29 , , . cord-264359-m9j3pcj1 78 1 and and CC cord-264359-m9j3pcj1 78 2 the the DT cord-264359-m9j3pcj1 78 3 results result NNS cord-264359-m9j3pcj1 78 4 are be VBP cord-264359-m9j3pcj1 78 5 shown show VBN cord-264359-m9j3pcj1 78 6 in in IN cord-264359-m9j3pcj1 78 7 Fig Fig NNP cord-264359-m9j3pcj1 78 8 . . . cord-264359-m9j3pcj1 79 1 4 4 LS cord-264359-m9j3pcj1 79 2 . . . cord-264359-m9j3pcj1 80 1 Both both DT cord-264359-m9j3pcj1 80 2 virus virus NN cord-264359-m9j3pcj1 80 3 strains strain NNS cord-264359-m9j3pcj1 80 4 yielded yield VBD cord-264359-m9j3pcj1 80 5 essentially essentially RB cord-264359-m9j3pcj1 80 6 the the DT cord-264359-m9j3pcj1 80 7 same same JJ cord-264359-m9j3pcj1 80 8 six six CD cord-264359-m9j3pcj1 80 9 - - HYPH cord-264359-m9j3pcj1 80 10 peak peak NN cord-264359-m9j3pcj1 80 11 pattern pattern NN cord-264359-m9j3pcj1 80 12 with with IN cord-264359-m9j3pcj1 80 13 minor minor JJ cord-264359-m9j3pcj1 80 14 differences difference NNS cord-264359-m9j3pcj1 80 15 : : : cord-264359-m9j3pcj1 80 16 ( ( -LRB- cord-264359-m9j3pcj1 80 17 i i NN cord-264359-m9j3pcj1 80 18 ) ) -RRB- cord-264359-m9j3pcj1 80 19 all all DT cord-264359-m9j3pcj1 80 20 MEF-1 MEF-1 NNP cord-264359-m9j3pcj1 80 21 components component NNS cord-264359-m9j3pcj1 80 22 migrated migrate VBD cord-264359-m9j3pcj1 80 23 slightly slightly RB cord-264359-m9j3pcj1 80 24 slower slow JJR cord-264359-m9j3pcj1 80 25 than than IN cord-264359-m9j3pcj1 80 26 their -PRON- PRP$ cord-264359-m9j3pcj1 80 27 la la NN cord-264359-m9j3pcj1 80 28 / / SYM cord-264359-m9j3pcj1 80 29 S3 S3 NNP cord-264359-m9j3pcj1 80 30 homologs homolog NNS cord-264359-m9j3pcj1 80 31 , , , cord-264359-m9j3pcj1 80 32 and and CC cord-264359-m9j3pcj1 80 33 ( ( -LRB- cord-264359-m9j3pcj1 80 34 ii ii NN cord-264359-m9j3pcj1 80 35 ) ) -RRB- cord-264359-m9j3pcj1 80 36 C2 C2 NNP cord-264359-m9j3pcj1 80 37 was be VBD cord-264359-m9j3pcj1 80 38 more more RBR cord-264359-m9j3pcj1 80 39 abundant abundant JJ cord-264359-m9j3pcj1 80 40 in in IN cord-264359-m9j3pcj1 80 41 MEF-1 MEF-1 NNP cord-264359-m9j3pcj1 80 42 than than IN cord-264359-m9j3pcj1 80 43 in in IN cord-264359-m9j3pcj1 80 44 la la NNP cord-264359-m9j3pcj1 80 45 / / SYM cord-264359-m9j3pcj1 80 46 S3 S3 NNP cord-264359-m9j3pcj1 80 47 protein protein NN cord-264359-m9j3pcj1 80 48 . . . cord-264359-m9j3pcj1 81 1 ations ation NNS cord-264359-m9j3pcj1 81 2 had have VBD cord-264359-m9j3pcj1 81 3 been be VBN cord-264359-m9j3pcj1 81 4 carried carry VBN cord-264359-m9j3pcj1 81 5 out out RP cord-264359-m9j3pcj1 81 6 at at IN cord-264359-m9j3pcj1 81 7 pH pH NNP cord-264359-m9j3pcj1 81 8 7.2 7.2 CD cord-264359-m9j3pcj1 81 9 ; ; : cord-264359-m9j3pcj1 81 10 ( ( -LRB- cord-264359-m9j3pcj1 81 11 ii ii NN cord-264359-m9j3pcj1 81 12 ) ) -RRB- cord-264359-m9j3pcj1 81 13 after after IN cord-264359-m9j3pcj1 81 14 a a DT cord-264359-m9j3pcj1 81 15 24-h 24-h JJ cord-264359-m9j3pcj1 81 16 pH ph NN cord-264359-m9j3pcj1 81 17 gradient gradient NN cord-264359-m9j3pcj1 81 18 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 81 19 , , , cord-264359-m9j3pcj1 81 20 the the DT cord-264359-m9j3pcj1 81 21 gel gel NN cord-264359-m9j3pcj1 81 22 segment segment NN cord-264359-m9j3pcj1 81 23 containing contain VBG cord-264359-m9j3pcj1 81 24 components component NNS cord-264359-m9j3pcj1 81 25 Cl Cl NNP cord-264359-m9j3pcj1 81 26 to to IN cord-264359-m9j3pcj1 81 27 C6 C6 NNP cord-264359-m9j3pcj1 81 28 was be VBD cord-264359-m9j3pcj1 81 29 eluted elute VBN cord-264359-m9j3pcj1 81 30 . . . cord-264359-m9j3pcj1 82 1 The the DT cord-264359-m9j3pcj1 82 2 eluate eluate NN cord-264359-m9j3pcj1 82 3 was be VBD cord-264359-m9j3pcj1 82 4 concentrated concentrate VBN cord-264359-m9j3pcj1 82 5 , , , cord-264359-m9j3pcj1 82 6 resubmitted resubmitte VBN cord-264359-m9j3pcj1 82 7 to to IN cord-264359-m9j3pcj1 82 8 the the DT cord-264359-m9j3pcj1 82 9 original original JJ cord-264359-m9j3pcj1 82 10 protein protein NN cord-264359-m9j3pcj1 82 11 extraction extraction NN cord-264359-m9j3pcj1 82 12 procedure procedure NN cord-264359-m9j3pcj1 82 13 , , , cord-264359-m9j3pcj1 82 14 and and CC cord-264359-m9j3pcj1 82 15 reelectrophoresed reelectrophorese VBN cord-264359-m9j3pcj1 82 16 in in IN cord-264359-m9j3pcj1 82 17 the the DT cord-264359-m9j3pcj1 82 18 normal normal JJ cord-264359-m9j3pcj1 82 19 system system NN cord-264359-m9j3pcj1 82 20 ; ; : cord-264359-m9j3pcj1 82 21 the the DT cord-264359-m9j3pcj1 82 22 results result NNS cord-264359-m9j3pcj1 82 23 were be VBD cord-264359-m9j3pcj1 82 24 similar similar JJ cord-264359-m9j3pcj1 82 25 to to IN cord-264359-m9j3pcj1 82 26 those those DT cord-264359-m9j3pcj1 82 27 shown show VBN cord-264359-m9j3pcj1 82 28 in in IN cord-264359-m9j3pcj1 82 29 Fig Fig NNP cord-264359-m9j3pcj1 82 30 . . . cord-264359-m9j3pcj1 83 1 2A 2A NNP cord-264359-m9j3pcj1 83 2 and and CC cord-264359-m9j3pcj1 83 3 identical identical JJ cord-264359-m9j3pcj1 83 4 to to IN cord-264359-m9j3pcj1 83 5 those those DT cord-264359-m9j3pcj1 83 6 of of IN cord-264359-m9j3pcj1 83 7 controls control NNS cord-264359-m9j3pcj1 83 8 in in IN cord-264359-m9j3pcj1 83 9 which which WDT cord-264359-m9j3pcj1 83 10 the the DT cord-264359-m9j3pcj1 83 11 first first JJ cord-264359-m9j3pcj1 83 12 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 83 13 was be VBD cord-264359-m9j3pcj1 83 14 omitted omit VBN cord-264359-m9j3pcj1 83 15 . . . cord-264359-m9j3pcj1 84 1 Thus thus RB cord-264359-m9j3pcj1 84 2 , , , cord-264359-m9j3pcj1 84 3 pretreatment pretreatment NN cord-264359-m9j3pcj1 84 4 of of IN cord-264359-m9j3pcj1 84 5 the the DT cord-264359-m9j3pcj1 84 6 SDS SDS NNP cord-264359-m9j3pcj1 84 7 complexes complex NNS cord-264359-m9j3pcj1 84 8 of of IN cord-264359-m9j3pcj1 84 9 poliovirion poliovirion NN cord-264359-m9j3pcj1 84 10 polypeptides polypeptide NNS cord-264359-m9j3pcj1 84 11 at at IN cord-264359-m9j3pcj1 84 12 pH pH NNP cord-264359-m9j3pcj1 84 13 11 11 CD cord-264359-m9j3pcj1 84 14 , , , cord-264359-m9j3pcj1 84 15 either either CC cord-264359-m9j3pcj1 84 16 in in IN cord-264359-m9j3pcj1 84 17 free free JJ cord-264359-m9j3pcj1 84 18 solution solution NN cord-264359-m9j3pcj1 84 19 or or CC cord-264359-m9j3pcj1 84 20 in in IN cord-264359-m9j3pcj1 84 21 an an DT cord-264359-m9j3pcj1 84 22 acrylamide acrylamide NN cord-264359-m9j3pcj1 84 23 gel gel NN cord-264359-m9j3pcj1 84 24 , , , cord-264359-m9j3pcj1 84 25 failed fail VBD cord-264359-m9j3pcj1 84 26 to to TO cord-264359-m9j3pcj1 84 27 produce produce VB cord-264359-m9j3pcj1 84 28 detectable detectable JJ cord-264359-m9j3pcj1 84 29 alterations alteration NNS cord-264359-m9j3pcj1 84 30 in in IN cord-264359-m9j3pcj1 84 31 their -PRON- PRP$ cord-264359-m9j3pcj1 84 32 migration migration NN cord-264359-m9j3pcj1 84 33 patterns pattern NNS cord-264359-m9j3pcj1 84 34 . . . cord-264359-m9j3pcj1 85 1 The the DT cord-264359-m9j3pcj1 85 2 experiments experiment NNS cord-264359-m9j3pcj1 85 3 to to TO cord-264359-m9j3pcj1 85 4 be be VB cord-264359-m9j3pcj1 85 5 presented present VBN cord-264359-m9j3pcj1 85 6 below below RB cord-264359-m9j3pcj1 85 7 , , , cord-264359-m9j3pcj1 85 8 in in IN cord-264359-m9j3pcj1 85 9 which which WDT cord-264359-m9j3pcj1 85 10 single single JJ cord-264359-m9j3pcj1 85 11 components component NNS cord-264359-m9j3pcj1 85 12 were be VBD cord-264359-m9j3pcj1 85 13 resolved resolve VBN cord-264359-m9j3pcj1 85 14 by by IN cord-264359-m9j3pcj1 85 15 pH pH NNP cord-264359-m9j3pcj1 85 16 gradient gradient NN cord-264359-m9j3pcj1 85 17 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 85 18 and and CC cord-264359-m9j3pcj1 85 19 rerun rerun NN cord-264359-m9j3pcj1 85 20 in in IN cord-264359-m9j3pcj1 85 21 the the DT cord-264359-m9j3pcj1 85 22 same same JJ cord-264359-m9j3pcj1 85 23 system system NN cord-264359-m9j3pcj1 85 24 , , , cord-264359-m9j3pcj1 85 25 allow allow VB cord-264359-m9j3pcj1 85 26 the the DT cord-264359-m9j3pcj1 85 27 same same JJ cord-264359-m9j3pcj1 85 28 conclusion conclusion NN cord-264359-m9j3pcj1 85 29 . . . cord-264359-m9j3pcj1 86 1 In in IN cord-264359-m9j3pcj1 86 2 addition addition NN cord-264359-m9j3pcj1 86 3 , , , cord-264359-m9j3pcj1 86 4 ( ( -LRB- cord-264359-m9j3pcj1 86 5 i i NN cord-264359-m9j3pcj1 86 6 ) ) -RRB- cord-264359-m9j3pcj1 86 7 incipient incipient JJ cord-264359-m9j3pcj1 86 8 resolution resolution NN cord-264359-m9j3pcj1 86 9 of of IN cord-264359-m9j3pcj1 86 10 VP2 vp2 NN cord-264359-m9j3pcj1 86 11 and and CC cord-264359-m9j3pcj1 86 12 VP3 vp3 NN cord-264359-m9j3pcj1 86 13 into into IN cord-264359-m9j3pcj1 86 14 doublets doublet NNS cord-264359-m9j3pcj1 86 15 was be VBD cord-264359-m9j3pcj1 86 16 also also RB cord-264359-m9j3pcj1 86 17 achieved achieve VBN cord-264359-m9j3pcj1 86 18 by by IN cord-264359-m9j3pcj1 86 19 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 86 20 at at IN cord-264359-m9j3pcj1 86 21 a a DT cord-264359-m9j3pcj1 86 22 lower low JJR cord-264359-m9j3pcj1 86 23 pH ph NN cord-264359-m9j3pcj1 86 24 , , , cord-264359-m9j3pcj1 86 25 including include VBG cord-264359-m9j3pcj1 86 26 the the DT cord-264359-m9j3pcj1 86 27 normal normal JJ cord-264359-m9j3pcj1 86 28 ( ( -LRB- cord-264359-m9j3pcj1 86 29 pH pH NNP cord-264359-m9j3pcj1 86 30 7.2 7.2 CD cord-264359-m9j3pcj1 86 31 ) ) -RRB- cord-264359-m9j3pcj1 86 32 technique technique NN cord-264359-m9j3pcj1 86 33 , , , cord-264359-m9j3pcj1 86 34 and and CC cord-264359-m9j3pcj1 86 35 ( ( -LRB- cord-264359-m9j3pcj1 86 36 ii ii NNP cord-264359-m9j3pcj1 86 37 ) ) -RRB- cord-264359-m9j3pcj1 86 38 nonviral nonviral JJ cord-264359-m9j3pcj1 86 39 test test NN cord-264359-m9j3pcj1 86 40 proteins protein NNS cord-264359-m9j3pcj1 86 41 failed fail VBD cord-264359-m9j3pcj1 86 42 to to TO cord-264359-m9j3pcj1 86 43 yield yield VB cord-264359-m9j3pcj1 86 44 multiple multiple JJ cord-264359-m9j3pcj1 86 45 components component NNS cord-264359-m9j3pcj1 86 46 in in IN cord-264359-m9j3pcj1 86 47 pH pH NNP cord-264359-m9j3pcj1 86 48 gradient gradient NN cord-264359-m9j3pcj1 86 49 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 86 50 ( ( -LRB- cord-264359-m9j3pcj1 86 51 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 86 52 and and CC cord-264359-m9j3pcj1 86 53 Boeye Boeye NNP cord-264359-m9j3pcj1 86 54 , , , cord-264359-m9j3pcj1 86 55 1978 1978 CD cord-264359-m9j3pcj1 86 56 ) ) -RRB- cord-264359-m9j3pcj1 86 57 . . . cord-264359-m9j3pcj1 87 1 It -PRON- PRP cord-264359-m9j3pcj1 87 2 is be VBZ cord-264359-m9j3pcj1 87 3 concluded conclude VBN cord-264359-m9j3pcj1 87 4 that that IN cord-264359-m9j3pcj1 87 5 pH pH NNP cord-264359-m9j3pcj1 87 6 gradient gradient NN cord-264359-m9j3pcj1 87 7 eiectrophoresis eiectrophoresis NNP cord-264359-m9j3pcj1 87 8 does do VBZ cord-264359-m9j3pcj1 87 9 not not RB cord-264359-m9j3pcj1 87 10 cause cause VB cord-264359-m9j3pcj1 87 11 the the DT cord-264359-m9j3pcj1 87 12 observed observed JJ cord-264359-m9j3pcj1 87 13 multiplicity multiplicity NN cord-264359-m9j3pcj1 87 14 of of IN cord-264359-m9j3pcj1 87 15 the the DT cord-264359-m9j3pcj1 87 16 poliovirus poliovirus NN cord-264359-m9j3pcj1 87 17 capsid capsid NN cord-264359-m9j3pcj1 87 18 polypeptides polypeptide NNS cord-264359-m9j3pcj1 87 19 but but CC cord-264359-m9j3pcj1 87 20 merely merely RB cord-264359-m9j3pcj1 87 21 resolves resolve VBZ cord-264359-m9j3pcj1 87 22 preexisting preexist VBG cord-264359-m9j3pcj1 87 23 components component NNS cord-264359-m9j3pcj1 87 24 . . . cord-264359-m9j3pcj1 88 1 In in IN cord-264359-m9j3pcj1 88 2 summary summary NN cord-264359-m9j3pcj1 88 3 , , , cord-264359-m9j3pcj1 88 4 fine fine JJ cord-264359-m9j3pcj1 88 5 resolution resolution NN cord-264359-m9j3pcj1 88 6 of of IN cord-264359-m9j3pcj1 88 7 the the DT cord-264359-m9j3pcj1 88 8 poliovirus poliovirus NN cord-264359-m9j3pcj1 88 9 capsid capsid NN cord-264359-m9j3pcj1 88 10 polypeptides polypeptide NNS cord-264359-m9j3pcj1 88 11 was be VBD cord-264359-m9j3pcj1 88 12 achieved achieve VBN cord-264359-m9j3pcj1 88 13 by by IN cord-264359-m9j3pcj1 88 14 pH pH NNP cord-264359-m9j3pcj1 88 15 gradient gradient NN cord-264359-m9j3pcj1 88 16 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 88 17 and and CC cord-264359-m9j3pcj1 88 18 the the DT cord-264359-m9j3pcj1 88 19 results result NNS cord-264359-m9j3pcj1 88 20 were be VBD cord-264359-m9j3pcj1 88 21 independent independent JJ cord-264359-m9j3pcj1 88 22 of of IN cord-264359-m9j3pcj1 88 23 ( ( -LRB- cord-264359-m9j3pcj1 88 24 i i NN cord-264359-m9j3pcj1 88 25 ) ) -RRB- cord-264359-m9j3pcj1 89 1 the the DT cord-264359-m9j3pcj1 89 2 virus virus NN cord-264359-m9j3pcj1 89 3 purification purification NN cord-264359-m9j3pcj1 89 4 or or CC cord-264359-m9j3pcj1 89 5 ( ( -LRB- cord-264359-m9j3pcj1 89 6 ii ii LS cord-264359-m9j3pcj1 89 7 ) ) -RRB- cord-264359-m9j3pcj1 89 8 protein protein NN cord-264359-m9j3pcj1 89 9 extraction extraction NN cord-264359-m9j3pcj1 89 10 procedures procedure NNS cord-264359-m9j3pcj1 89 11 , , , cord-264359-m9j3pcj1 89 12 ( ( -LRB- cord-264359-m9j3pcj1 89 13 iii iii NN cord-264359-m9j3pcj1 89 14 ) ) -RRB- cord-264359-m9j3pcj1 90 1 the the DT cord-264359-m9j3pcj1 90 2 state state NN cord-264359-m9j3pcj1 90 3 of of IN cord-264359-m9j3pcj1 90 4 maturation maturation NN cord-264359-m9j3pcj1 90 5 ( ( -LRB- cord-264359-m9j3pcj1 90 6 procapsid procapsid JJ cord-264359-m9j3pcj1 90 7 or or CC cord-264359-m9j3pcj1 90 8 mature mature JJ cord-264359-m9j3pcj1 90 9 virion virion NN cord-264359-m9j3pcj1 90 10 ) ) -RRB- cord-264359-m9j3pcj1 90 11 , , , cord-264359-m9j3pcj1 90 12 and and CC cord-264359-m9j3pcj1 90 13 ( ( -LRB- cord-264359-m9j3pcj1 90 14 iv iv LS cord-264359-m9j3pcj1 90 15 ) ) -RRB- cord-264359-m9j3pcj1 90 16 the the DT cord-264359-m9j3pcj1 90 17 serotype serotype NN cord-264359-m9j3pcj1 90 18 ( ( -LRB- cord-264359-m9j3pcj1 90 19 type type NN cord-264359-m9j3pcj1 90 20 1 1 CD cord-264359-m9j3pcj1 90 21 or or CC cord-264359-m9j3pcj1 90 22 2 2 CD cord-264359-m9j3pcj1 90 23 ) ) -RRB- cord-264359-m9j3pcj1 90 24 . . . cord-264359-m9j3pcj1 91 1 Origin origin NN cord-264359-m9j3pcj1 91 2 of of IN cord-264359-m9j3pcj1 91 3 the the DT cord-264359-m9j3pcj1 91 4 Multiple Multiple NNP cord-264359-m9j3pcj1 91 5 Origin Origin NNP cord-264359-m9j3pcj1 91 6 of of IN cord-264359-m9j3pcj1 91 7 the the DT cord-264359-m9j3pcj1 91 8 Components component NNS cord-264359-m9j3pcj1 91 9 Resolved resolve VBN cord-264359-m9j3pcj1 91 10 in in IN cord-264359-m9j3pcj1 91 11 pH pH NNP cord-264359-m9j3pcj1 91 12 Components Components NNP cord-264359-m9j3pcj1 91 13 in in IN cord-264359-m9j3pcj1 91 14 pH pH NNP cord-264359-m9j3pcj1 91 15 Gradient Gradient NNP cord-264359-m9j3pcj1 91 16 Electrophoresis Electrophoresis NNP cord-264359-m9j3pcj1 91 17 Since since IN cord-264359-m9j3pcj1 91 18 the the DT cord-264359-m9j3pcj1 91 19 migration migration NN cord-264359-m9j3pcj1 91 20 of of IN cord-264359-m9j3pcj1 91 21 the the DT cord-264359-m9j3pcj1 91 22 poliovirus poliovirus NN cord-264359-m9j3pcj1 91 23 Gradient Gradient NNP cord-264359-m9j3pcj1 91 24 Ebctrophoresis Ebctrophoresis NNP cord-264359-m9j3pcj1 91 25 polypeptides polypeptide NNS cord-264359-m9j3pcj1 91 26 in in IN cord-264359-m9j3pcj1 91 27 pH pH NNP cord-264359-m9j3pcj1 91 28 gradient gradient NN cord-264359-m9j3pcj1 91 29 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 91 30 takes take VBZ cord-264359-m9j3pcj1 91 31 place place NN cord-264359-m9j3pcj1 91 32 at at IN cord-264359-m9j3pcj1 91 33 about about IN cord-264359-m9j3pcj1 91 34 pH pH NNP cord-264359-m9j3pcj1 91 35 10.5 10.5 CD cord-264359-m9j3pcj1 91 36 to to IN cord-264359-m9j3pcj1 91 37 11 11 CD cord-264359-m9j3pcj1 91 38 ( ( -LRB- cord-264359-m9j3pcj1 91 39 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 91 40 and and CC cord-264359-m9j3pcj1 91 41 Boeye Boeye NNP cord-264359-m9j3pcj1 91 42 , , , cord-264359-m9j3pcj1 91 43 1978 1978 CD cord-264359-m9j3pcj1 91 44 ) ) -RRB- cord-264359-m9j3pcj1 91 45 , , , cord-264359-m9j3pcj1 91 46 one one PRP cord-264359-m9j3pcj1 91 47 may may MD cord-264359-m9j3pcj1 91 48 well well RB cord-264359-m9j3pcj1 91 49 wonder wonder VB cord-264359-m9j3pcj1 91 50 whether whether IN cord-264359-m9j3pcj1 91 51 this this DT cord-264359-m9j3pcj1 91 52 alkaline alkaline JJ cord-264359-m9j3pcj1 91 53 pH ph NN cord-264359-m9j3pcj1 91 54 does do VBZ cord-264359-m9j3pcj1 91 55 not not RB cord-264359-m9j3pcj1 91 56 cause cause VB cord-264359-m9j3pcj1 91 57 partial partial JJ cord-264359-m9j3pcj1 91 58 degradation degradation NN cord-264359-m9j3pcj1 91 59 of of IN cord-264359-m9j3pcj1 91 60 the the DT cord-264359-m9j3pcj1 91 61 polypeptides polypeptide NNS cord-264359-m9j3pcj1 91 62 . . . cord-264359-m9j3pcj1 92 1 The the DT cord-264359-m9j3pcj1 92 2 following follow VBG cord-264359-m9j3pcj1 92 3 experiments experiment NNS cord-264359-m9j3pcj1 92 4 were be VBD cord-264359-m9j3pcj1 92 5 performed perform VBN cord-264359-m9j3pcj1 92 6 to to TO cord-264359-m9j3pcj1 92 7 detect detect VB cord-264359-m9j3pcj1 92 8 possible possible JJ cord-264359-m9j3pcj1 92 9 alkaline alkaline JJ cord-264359-m9j3pcj1 92 10 degradation degradation NN cord-264359-m9j3pcj1 92 11 : : : cord-264359-m9j3pcj1 92 12 ( ( -LRB- cord-264359-m9j3pcj1 92 13 i i NN cord-264359-m9j3pcj1 92 14 ) ) -RRB- cord-264359-m9j3pcj1 92 15 poliovirion poliovirion NNP cord-264359-m9j3pcj1 92 16 protein protein NN cord-264359-m9j3pcj1 92 17 was be VBD cord-264359-m9j3pcj1 92 18 dialyzed dialyze VBN cord-264359-m9j3pcj1 92 19 for for IN cord-264359-m9j3pcj1 92 20 24 24 CD cord-264359-m9j3pcj1 92 21 hr hr NN cord-264359-m9j3pcj1 92 22 at at IN cord-264359-m9j3pcj1 92 23 room room NN cord-264359-m9j3pcj1 92 24 temperature temperature NN cord-264359-m9j3pcj1 92 25 against against IN cord-264359-m9j3pcj1 92 26 a a DT cord-264359-m9j3pcj1 92 27 mixture mixture NN cord-264359-m9j3pcj1 92 28 of of IN cord-264359-m9j3pcj1 92 29 1 1 CD cord-264359-m9j3pcj1 92 30 % % NN cord-264359-m9j3pcj1 92 31 mercaptoethanol mercaptoethanol NNS cord-264359-m9j3pcj1 92 32 and and CC cord-264359-m9j3pcj1 92 33 1 1 CD cord-264359-m9j3pcj1 92 34 % % NN cord-264359-m9j3pcj1 92 35 SDS SDS NNP cord-264359-m9j3pcj1 92 36 in in IN cord-264359-m9j3pcj1 92 37 0.01 0.01 CD cord-264359-m9j3pcj1 92 38 M m NN cord-264359-m9j3pcj1 92 39 phosphate phosphate NN cord-264359-m9j3pcj1 92 40 buffer buffer NN cord-264359-m9j3pcj1 92 41 at at IN cord-264359-m9j3pcj1 92 42 pH pH NNP cord-264359-m9j3pcj1 92 43 11.0 11.0 CD cord-264359-m9j3pcj1 92 44 ; ; : cord-264359-m9j3pcj1 92 45 the the DT cord-264359-m9j3pcj1 92 46 pH pH NNP cord-264359-m9j3pcj1 92 47 was be VBD cord-264359-m9j3pcj1 92 48 then then RB cord-264359-m9j3pcj1 92 49 lowered lower VBN cord-264359-m9j3pcj1 92 50 to to IN cord-264359-m9j3pcj1 92 51 7.2 7.2 CD cord-264359-m9j3pcj1 92 52 , , , cord-264359-m9j3pcj1 92 53 the the DT cord-264359-m9j3pcj1 92 54 extraction extraction NN cord-264359-m9j3pcj1 92 55 procedure procedure NN cord-264359-m9j3pcj1 92 56 was be VBD cord-264359-m9j3pcj1 92 57 repeated repeat VBN cord-264359-m9j3pcj1 92 58 , , , cord-264359-m9j3pcj1 92 59 and and CC cord-264359-m9j3pcj1 92 60 the the DT cord-264359-m9j3pcj1 92 61 polypeptides polypeptide NNS cord-264359-m9j3pcj1 92 62 were be VBD cord-264359-m9j3pcj1 92 63 electrophoresed electrophorese VBN cord-264359-m9j3pcj1 92 64 using use VBG cord-264359-m9j3pcj1 92 65 the the DT cord-264359-m9j3pcj1 92 66 normal normal JJ cord-264359-m9j3pcj1 92 67 technique technique NN cord-264359-m9j3pcj1 92 68 . . . cord-264359-m9j3pcj1 93 1 The the DT cord-264359-m9j3pcj1 93 2 polypeptide polypeptide NN cord-264359-m9j3pcj1 93 3 pattern pattern NN cord-264359-m9j3pcj1 93 4 obtained obtain VBN cord-264359-m9j3pcj1 93 5 after after IN cord-264359-m9j3pcj1 93 6 this this DT cord-264359-m9j3pcj1 93 7 alkaline alkaline JJ cord-264359-m9j3pcj1 93 8 pretreatment pretreatment NN cord-264359-m9j3pcj1 93 9 was be VBD cord-264359-m9j3pcj1 93 10 similar similar JJ cord-264359-m9j3pcj1 93 11 to to IN cord-264359-m9j3pcj1 93 12 that that DT cord-264359-m9j3pcj1 93 13 of of IN cord-264359-m9j3pcj1 93 14 Fig Fig NNP cord-264359-m9j3pcj1 93 15 . . . cord-264359-m9j3pcj1 93 16 2A 2a CD cord-264359-m9j3pcj1 93 17 and and CC cord-264359-m9j3pcj1 93 18 indistinguishable indistinguishable JJ cord-264359-m9j3pcj1 93 19 from from IN cord-264359-m9j3pcj1 93 20 that that DT cord-264359-m9j3pcj1 93 21 of of IN cord-264359-m9j3pcj1 93 22 a a DT cord-264359-m9j3pcj1 93 23 control control NN cord-264359-m9j3pcj1 93 24 where where WRB cord-264359-m9j3pcj1 93 25 all all DT cord-264359-m9j3pcj1 93 26 oper oper NN cord-264359-m9j3pcj1 93 27 - - HYPH cord-264359-m9j3pcj1 93 28 Two two CD cord-264359-m9j3pcj1 93 29 kinds kind NNS cord-264359-m9j3pcj1 93 30 of of IN cord-264359-m9j3pcj1 93 31 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 93 32 experiments experiment NNS cord-264359-m9j3pcj1 93 33 were be VBD cord-264359-m9j3pcj1 93 34 performed perform VBN cord-264359-m9j3pcj1 93 35 . . . cord-264359-m9j3pcj1 94 1 First first RB cord-264359-m9j3pcj1 94 2 , , , cord-264359-m9j3pcj1 94 3 tritiated tritiated JJ cord-264359-m9j3pcj1 94 4 poliovirion poliovirion NN cord-264359-m9j3pcj1 94 5 protein protein NN cord-264359-m9j3pcj1 94 6 was be VBD cord-264359-m9j3pcj1 94 7 electrophoresed electrophorese VBN cord-264359-m9j3pcj1 94 8 using use VBG cord-264359-m9j3pcj1 94 9 the the DT cord-264359-m9j3pcj1 94 10 normal normal JJ cord-264359-m9j3pcj1 94 11 technique technique NN cord-264359-m9j3pcj1 94 12 , , , cord-264359-m9j3pcj1 94 13 the the DT cord-264359-m9j3pcj1 94 14 gel gel NN cord-264359-m9j3pcj1 94 15 being be VBG cord-264359-m9j3pcj1 94 16 sliced slice VBN cord-264359-m9j3pcj1 94 17 and and CC cord-264359-m9j3pcj1 94 18 eluted elute VBN cord-264359-m9j3pcj1 94 19 . . . cord-264359-m9j3pcj1 95 1 Aliquots Aliquots NNP cord-264359-m9j3pcj1 95 2 of of IN cord-264359-m9j3pcj1 95 3 the the DT cord-264359-m9j3pcj1 95 4 slice slice NN cord-264359-m9j3pcj1 95 5 eluates eluate NNS cord-264359-m9j3pcj1 95 6 were be VBD cord-264359-m9j3pcj1 95 7 used use VBN cord-264359-m9j3pcj1 95 8 to to TO cord-264359-m9j3pcj1 95 9 determine determine VB cord-264359-m9j3pcj1 95 10 the the DT cord-264359-m9j3pcj1 95 11 radioactivity radioactivity NN cord-264359-m9j3pcj1 95 12 profile profile NN cord-264359-m9j3pcj1 95 13 ( ( -LRB- cord-264359-m9j3pcj1 95 14 Fig fig NN cord-264359-m9j3pcj1 95 15 . . . cord-264359-m9j3pcj1 95 16 50 50 CD cord-264359-m9j3pcj1 95 17 ) ) -RRB- cord-264359-m9j3pcj1 95 18 and and CC cord-264359-m9j3pcj1 95 19 the the DT cord-264359-m9j3pcj1 95 20 remainder remainder NN cord-264359-m9j3pcj1 95 21 was be VBD cord-264359-m9j3pcj1 95 22 combined combine VBN cord-264359-m9j3pcj1 95 23 into into IN cord-264359-m9j3pcj1 95 24 pools pool NNS cord-264359-m9j3pcj1 95 25 corresponding correspond VBG cord-264359-m9j3pcj1 95 26 to to IN cord-264359-m9j3pcj1 95 27 peaks peak NNS cord-264359-m9j3pcj1 95 28 VPl vpl NN cord-264359-m9j3pcj1 95 29 , , , cord-264359-m9j3pcj1 95 30 VP2 vp2 NN cord-264359-m9j3pcj1 95 31 , , , cord-264359-m9j3pcj1 95 32 and and CC cord-264359-m9j3pcj1 95 33 VP3 VP3 NNP cord-264359-m9j3pcj1 95 34 and and CC cord-264359-m9j3pcj1 95 35 to to IN cord-264359-m9j3pcj1 95 36 the the DT cord-264359-m9j3pcj1 95 37 regions region NNS cord-264359-m9j3pcj1 95 38 in in IN cord-264359-m9j3pcj1 95 39 between between IN cord-264359-m9j3pcj1 95 40 these these DT cord-264359-m9j3pcj1 95 41 peaks peak NNS cord-264359-m9j3pcj1 95 42 . . . cord-264359-m9j3pcj1 96 1 14C 14c LS cord-264359-m9j3pcj1 96 2 - - HYPH cord-264359-m9j3pcj1 96 3 Labeled label VBN cord-264359-m9j3pcj1 96 4 virus virus NN cord-264359-m9j3pcj1 96 5 was be VBD cord-264359-m9j3pcj1 96 6 added add VBN cord-264359-m9j3pcj1 96 7 to to IN cord-264359-m9j3pcj1 96 8 each each DT cord-264359-m9j3pcj1 96 9 pool pool NN cord-264359-m9j3pcj1 96 10 , , , cord-264359-m9j3pcj1 96 11 and and CC cord-264359-m9j3pcj1 96 12 the the DT cord-264359-m9j3pcj1 96 13 mixture mixture NN cord-264359-m9j3pcj1 96 14 was be VBD cord-264359-m9j3pcj1 96 15 extracted extract VBN cord-264359-m9j3pcj1 96 16 and and CC cord-264359-m9j3pcj1 96 17 reelectrophoresed reelectrophorese VBN cord-264359-m9j3pcj1 96 18 using use VBG cord-264359-m9j3pcj1 96 19 both both CC cord-264359-m9j3pcj1 96 20 the the DT cord-264359-m9j3pcj1 96 21 normal normal JJ cord-264359-m9j3pcj1 96 22 and and CC cord-264359-m9j3pcj1 96 23 pH ph NN cord-264359-m9j3pcj1 96 24 gradient gradient NN cord-264359-m9j3pcj1 96 25 technique technique NN cord-264359-m9j3pcj1 96 26 . . . cord-264359-m9j3pcj1 97 1 As as IN cord-264359-m9j3pcj1 97 2 shown show VBN cord-264359-m9j3pcj1 97 3 in in IN cord-264359-m9j3pcj1 97 4 Fig Fig NNP cord-264359-m9j3pcj1 97 5 . . . cord-264359-m9j3pcj1 98 1 5A 5A NNP cord-264359-m9j3pcj1 98 2 -5C -5C NNP cord-264359-m9j3pcj1 98 3 , , , cord-264359-m9j3pcj1 99 1 the the DT cord-264359-m9j3pcj1 99 2 pools pool NNS cord-264359-m9j3pcj1 99 3 corresponding correspond VBG cord-264359-m9j3pcj1 99 4 to to IN cord-264359-m9j3pcj1 99 5 VPl VPl NNP cord-264359-m9j3pcj1 99 6 , , , cord-264359-m9j3pcj1 99 7 VP2 vp2 NN cord-264359-m9j3pcj1 99 8 , , , cord-264359-m9j3pcj1 99 9 and and CC cord-264359-m9j3pcj1 99 10 VP3 VP3 NNP cord-264359-m9j3pcj1 99 11 each each DT cord-264359-m9j3pcj1 99 12 yielded yield VBD cord-264359-m9j3pcj1 99 13 a a DT cord-264359-m9j3pcj1 99 14 single single JJ cord-264359-m9j3pcj1 99 15 peak peak NN cord-264359-m9j3pcj1 99 16 under under IN cord-264359-m9j3pcj1 99 17 normal normal JJ cord-264359-m9j3pcj1 99 18 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 99 19 ; ; : cord-264359-m9j3pcj1 99 20 the the DT cord-264359-m9j3pcj1 99 21 intermediate intermediate JJ cord-264359-m9j3pcj1 99 22 fractions fraction NNS cord-264359-m9j3pcj1 99 23 , , , cord-264359-m9j3pcj1 99 24 as as IN cord-264359-m9j3pcj1 99 25 expected expect VBN cord-264359-m9j3pcj1 99 26 , , , cord-264359-m9j3pcj1 99 27 yielded yield VBD cord-264359-m9j3pcj1 99 28 two two CD cord-264359-m9j3pcj1 99 29 peaks peak NNS cord-264359-m9j3pcj1 99 30 ( ( -LRB- cord-264359-m9j3pcj1 99 31 results result NNS cord-264359-m9j3pcj1 99 32 not not RB cord-264359-m9j3pcj1 99 33 shown show VBN cord-264359-m9j3pcj1 99 34 ) ) -RRB- cord-264359-m9j3pcj1 99 35 . . . cord-264359-m9j3pcj1 100 1 The the DT cord-264359-m9j3pcj1 100 2 VP1 vp1 NN cord-264359-m9j3pcj1 100 3 material material NN cord-264359-m9j3pcj1 100 4 in in IN cord-264359-m9j3pcj1 100 5 pH pH NNP cord-264359-m9j3pcj1 100 6 gradient gradient NNP cord-264359-m9j3pcj1 100 7 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 100 8 yielded yield VBD cord-264359-m9j3pcj1 100 9 mainly mainly RB cord-264359-m9j3pcj1 100 10 Cl Cl NNP cord-264359-m9j3pcj1 100 11 ( ( -LRB- cord-264359-m9j3pcj1 100 12 Fig Fig NNP cord-264359-m9j3pcj1 100 13 . . . cord-264359-m9j3pcj1 100 14 5D 5D NNP cord-264359-m9j3pcj1 100 15 ) ) -RRB- cord-264359-m9j3pcj1 101 1 FIG FIG NNP cord-264359-m9j3pcj1 101 2 . . . cord-264359-m9j3pcj1 102 1 5 5 LS cord-264359-m9j3pcj1 102 2 . . . cord-264359-m9j3pcj1 103 1 Normal normal JJ cord-264359-m9j3pcj1 103 2 and and CC cord-264359-m9j3pcj1 103 3 pH pH NNP cord-264359-m9j3pcj1 103 4 gradient gradient NNP cord-264359-m9j3pcj1 103 5 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 103 6 of of IN cord-264359-m9j3pcj1 103 7 VPl VPl NNP cord-264359-m9j3pcj1 103 8 , , , cord-264359-m9j3pcj1 103 9 VP2 vp2 NN cord-264359-m9j3pcj1 103 10 , , , cord-264359-m9j3pcj1 103 11 and and CC cord-264359-m9j3pcj1 103 12 VP3 VP3 NNP cord-264359-m9j3pcj1 103 13 ( ( -LRB- cord-264359-m9j3pcj1 103 14 strain strain NN cord-264359-m9j3pcj1 103 15 la la NNP cord-264359-m9j3pcj1 103 16 / / SYM cord-264359-m9j3pcj1 103 17 S3 S3 NNP cord-264359-m9j3pcj1 103 18 ) ) -RRB- cord-264359-m9j3pcj1 103 19 . . . cord-264359-m9j3pcj1 104 1 ( ( -LRB- cord-264359-m9j3pcj1 104 2 0 0 NFP cord-264359-m9j3pcj1 104 3 ) ) -RRB- cord-264359-m9j3pcj1 105 1 Primary primary JJ cord-264359-m9j3pcj1 105 2 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 105 3 of of IN cord-264359-m9j3pcj1 105 4 " " `` cord-264359-m9j3pcj1 105 5 H h NN cord-264359-m9j3pcj1 105 6 - - HYPH cord-264359-m9j3pcj1 105 7 labeled label VBN cord-264359-m9j3pcj1 105 8 la la NNP cord-264359-m9j3pcj1 105 9 / / SYM cord-264359-m9j3pcj1 105 10 S3 s3 NN cord-264359-m9j3pcj1 105 11 virion virion NN cord-264359-m9j3pcj1 105 12 protein protein NN cord-264359-m9j3pcj1 105 13 ( ( -LRB- cord-264359-m9j3pcj1 105 14 normal normal JJ cord-264359-m9j3pcj1 105 15 technique technique NN cord-264359-m9j3pcj1 105 16 , , , cord-264359-m9j3pcj1 105 17 24 24 CD cord-264359-m9j3pcj1 105 18 hr hr NN cord-264359-m9j3pcj1 105 19 ) ) -RRB- cord-264359-m9j3pcj1 105 20 . . . cord-264359-m9j3pcj1 106 1 Slices slice NNS cord-264359-m9j3pcj1 106 2 were be VBD cord-264359-m9j3pcj1 106 3 eluted elute VBN cord-264359-m9j3pcj1 106 4 in in IN cord-264359-m9j3pcj1 106 5 0.5 0.5 CD cord-264359-m9j3pcj1 106 6 ml ml NNS cord-264359-m9j3pcj1 106 7 of of IN cord-264359-m9j3pcj1 106 8 0.1 0.1 CD cord-264359-m9j3pcj1 106 9 % % NN cord-264359-m9j3pcj1 106 10 SDS SDS NNP cord-264359-m9j3pcj1 106 11 , , , cord-264359-m9j3pcj1 106 12 of of IN cord-264359-m9j3pcj1 106 13 which which WDT cord-264359-m9j3pcj1 106 14 20 20 CD cord-264359-m9j3pcj1 106 15 1.11 1.11 CD cord-264359-m9j3pcj1 106 16 was be VBD cord-264359-m9j3pcj1 106 17 used use VBN cord-264359-m9j3pcj1 106 18 to to TO cord-264359-m9j3pcj1 106 19 determine determine VB cord-264359-m9j3pcj1 106 20 the the DT cord-264359-m9j3pcj1 106 21 radioactivity radioactivity NN cord-264359-m9j3pcj1 106 22 profile profile NN cord-264359-m9j3pcj1 106 23 . . . cord-264359-m9j3pcj1 107 1 Full full JJ cord-264359-m9j3pcj1 107 2 circles circle NNS cord-264359-m9j3pcj1 107 3 show show VBP cord-264359-m9j3pcj1 107 4 fractions fraction NNS cord-264359-m9j3pcj1 107 5 that that WDT cord-264359-m9j3pcj1 107 6 were be VBD cord-264359-m9j3pcj1 107 7 subsequently subsequently RB cord-264359-m9j3pcj1 107 8 joined join VBN cord-264359-m9j3pcj1 107 9 into into IN cord-264359-m9j3pcj1 107 10 three three CD cord-264359-m9j3pcj1 107 11 pools pool NNS cord-264359-m9j3pcj1 107 12 ( ( -LRB- cord-264359-m9j3pcj1 107 13 representing represent VBG cord-264359-m9j3pcj1 107 14 VPl VPl NNP cord-264359-m9j3pcj1 107 15 , , , cord-264359-m9j3pcj1 107 16 VP2 vp2 NN cord-264359-m9j3pcj1 107 17 , , , cord-264359-m9j3pcj1 107 18 and and CC cord-264359-m9j3pcj1 107 19 VP3 VP3 NNP cord-264359-m9j3pcj1 107 20 ) ) -RRB- cord-264359-m9j3pcj1 107 21 for for IN cord-264359-m9j3pcj1 107 22 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 107 23 ; ; : cord-264359-m9j3pcj1 107 24 40-to 40-to CD cord-264359-m9j3pcj1 107 25 60 60 CD cord-264359-m9j3pcj1 107 26 - - HYPH cord-264359-m9j3pcj1 107 27 1.11 1.11 CD cord-264359-m9j3pcj1 107 28 samples sample NNS cord-264359-m9j3pcj1 107 29 of of IN cord-264359-m9j3pcj1 107 30 these these DT cord-264359-m9j3pcj1 107 31 pools pool NNS cord-264359-m9j3pcj1 107 32 were be VBD cord-264359-m9j3pcj1 107 33 mixed mix VBN cord-264359-m9j3pcj1 107 34 with with IN cord-264359-m9j3pcj1 107 35 " " `` cord-264359-m9j3pcj1 107 36 C c NN cord-264359-m9j3pcj1 107 37 - - HYPH cord-264359-m9j3pcj1 107 38 virus virus NN cord-264359-m9j3pcj1 107 39 , , , cord-264359-m9j3pcj1 107 40 submitted submit VBN cord-264359-m9j3pcj1 107 41 to to IN cord-264359-m9j3pcj1 107 42 the the DT cord-264359-m9j3pcj1 107 43 protein protein NN cord-264359-m9j3pcj1 107 44 extraction extraction NN cord-264359-m9j3pcj1 107 45 procedure procedure NN cord-264359-m9j3pcj1 107 46 , , , cord-264359-m9j3pcj1 107 47 and and CC cord-264359-m9j3pcj1 107 48 reelectrophoresed reelectrophorese VBD cord-264359-m9j3pcj1 107 49 simultaneously simultaneously RB cord-264359-m9j3pcj1 107 50 in in IN cord-264359-m9j3pcj1 107 51 the the DT cord-264359-m9j3pcj1 107 52 normal normal JJ cord-264359-m9j3pcj1 107 53 and and CC cord-264359-m9j3pcj1 107 54 pH ph NN cord-264359-m9j3pcj1 107 55 gradient gradient NN cord-264359-m9j3pcj1 107 56 system system NN cord-264359-m9j3pcj1 107 57 . . . cord-264359-m9j3pcj1 108 1 material material NN cord-264359-m9j3pcj1 108 2 yielded yield VBD cord-264359-m9j3pcj1 108 3 a a DT cord-264359-m9j3pcj1 108 4 broad broad JJ cord-264359-m9j3pcj1 108 5 peak peak NN cord-264359-m9j3pcj1 108 6 covering cover VBG cord-264359-m9j3pcj1 108 7 the the DT cord-264359-m9j3pcj1 108 8 ity ity NN cord-264359-m9j3pcj1 108 9 , , , cord-264359-m9j3pcj1 108 10 no no DT cord-264359-m9j3pcj1 108 11 conclusive conclusive JJ cord-264359-m9j3pcj1 108 12 results result NNS cord-264359-m9j3pcj1 108 13 were be VBD cord-264359-m9j3pcj1 108 14 obtained obtain VBN cord-264359-m9j3pcj1 108 15 whole whole JJ cord-264359-m9j3pcj1 108 16 C3-C4 C3-C4 NNP cord-264359-m9j3pcj1 108 17 area area NN cord-264359-m9j3pcj1 108 18 ( ( -LRB- cord-264359-m9j3pcj1 108 19 Fig Fig NNP cord-264359-m9j3pcj1 108 20 . . . cord-264359-m9j3pcj1 108 21 5E 5E NNP cord-264359-m9j3pcj1 108 22 ) ) -RRB- cord-264359-m9j3pcj1 108 23 , , , cord-264359-m9j3pcj1 108 24 and and CC cord-264359-m9j3pcj1 108 25 the the DT cord-264359-m9j3pcj1 108 26 VP3 VP3 NNP cord-264359-m9j3pcj1 108 27 with with IN cord-264359-m9j3pcj1 108 28 C2 C2 NNP cord-264359-m9j3pcj1 108 29 or or CC cord-264359-m9j3pcj1 108 30 C4 C4 NNP cord-264359-m9j3pcj1 108 31 ) ) -RRB- cord-264359-m9j3pcj1 108 32 . . . cord-264359-m9j3pcj1 109 1 material material NN cord-264359-m9j3pcj1 109 2 yielded yield VBD cord-264359-m9j3pcj1 109 3 both both CC cord-264359-m9j3pcj1 109 4 C5 C5 NNP cord-264359-m9j3pcj1 109 5 and and CC cord-264359-m9j3pcj1 109 6 C6 C6 NNP cord-264359-m9j3pcj1 109 7 ( ( -LRB- cord-264359-m9j3pcj1 109 8 Fig Fig NNP cord-264359-m9j3pcj1 109 9 . . . cord-264359-m9j3pcj1 109 10 5F 5F NNP cord-264359-m9j3pcj1 109 11 ) ) -RRB- cord-264359-m9j3pcj1 109 12 . . . cord-264359-m9j3pcj1 110 1 Taken take VBN cord-264359-m9j3pcj1 110 2 together together RB cord-264359-m9j3pcj1 110 3 , , , cord-264359-m9j3pcj1 110 4 the the DT cord-264359-m9j3pcj1 110 5 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 110 6 ex ex NN cord-264359-m9j3pcj1 110 7 - - : cord-264359-m9j3pcj1 110 8 The the DT cord-264359-m9j3pcj1 110 9 reverse reverse JJ cord-264359-m9j3pcj1 110 10 kind kind NN cord-264359-m9j3pcj1 110 11 of of IN cord-264359-m9j3pcj1 110 12 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 110 13 was be VBD cord-264359-m9j3pcj1 110 14 periments periment NNS cord-264359-m9j3pcj1 110 15 establish establish VBP cord-264359-m9j3pcj1 110 16 the the DT cord-264359-m9j3pcj1 110 17 electrophoretic electrophoretic NN cord-264359-m9j3pcj1 110 18 also also RB cord-264359-m9j3pcj1 110 19 performed perform VBN cord-264359-m9j3pcj1 110 20 . . . cord-264359-m9j3pcj1 111 1 In in IN cord-264359-m9j3pcj1 111 2 this this DT cord-264359-m9j3pcj1 111 3 case case NN cord-264359-m9j3pcj1 111 4 , , , cord-264359-m9j3pcj1 111 5 the the DT cord-264359-m9j3pcj1 111 6 protein protein NN cord-264359-m9j3pcj1 111 7 equivalence equivalence NN cord-264359-m9j3pcj1 111 8 of of IN cord-264359-m9j3pcj1 111 9 Cl Cl NNP cord-264359-m9j3pcj1 111 10 and and CC cord-264359-m9j3pcj1 111 11 VPl VPl NNP cord-264359-m9j3pcj1 111 12 , , , cord-264359-m9j3pcj1 111 13 which which WDT cord-264359-m9j3pcj1 111 14 means mean VBZ cord-264359-m9j3pcj1 111 15 was be VBD cord-264359-m9j3pcj1 111 16 first first RB cord-264359-m9j3pcj1 111 17 submitted submit VBN cord-264359-m9j3pcj1 111 18 to to IN cord-264359-m9j3pcj1 111 19 pH pH NNP cord-264359-m9j3pcj1 111 20 gradient gradient NNP cord-264359-m9j3pcj1 111 21 electrothat electrothat NN cord-264359-m9j3pcj1 111 22 the the DT cord-264359-m9j3pcj1 111 23 same same JJ cord-264359-m9j3pcj1 111 24 molecules molecule NNS cord-264359-m9j3pcj1 111 25 which which WDT cord-264359-m9j3pcj1 111 26 migrate migrate VBP cord-264359-m9j3pcj1 111 27 as as IN cord-264359-m9j3pcj1 111 28 phoresis phoresis NNP cord-264359-m9j3pcj1 111 29 . . . cord-264359-m9j3pcj1 112 1 The the DT cord-264359-m9j3pcj1 112 2 excised excise VBN cord-264359-m9j3pcj1 112 3 peaks peak NNS cord-264359-m9j3pcj1 112 4 were be VBD cord-264359-m9j3pcj1 112 5 rerun rerun VBN cord-264359-m9j3pcj1 112 6 in in IN cord-264359-m9j3pcj1 112 7 Cl Cl NNP cord-264359-m9j3pcj1 112 8 in in IN cord-264359-m9j3pcj1 113 1 pH pH NNP cord-264359-m9j3pcj1 113 2 gradient gradient NNP cord-264359-m9j3pcj1 113 3 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 113 4 migrate migrate VBP cord-264359-m9j3pcj1 113 5 the the DT cord-264359-m9j3pcj1 113 6 pH pH NNP cord-264359-m9j3pcj1 113 7 gradient gradient NN cord-264359-m9j3pcj1 113 8 system system NN cord-264359-m9j3pcj1 113 9 for for IN cord-264359-m9j3pcj1 113 10 further further JJ cord-264359-m9j3pcj1 113 11 purifias purifias NNP cord-264359-m9j3pcj1 113 12 VP1 VP1 NNP cord-264359-m9j3pcj1 113 13 using use VBG cord-264359-m9j3pcj1 113 14 normal normal JJ cord-264359-m9j3pcj1 113 15 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 113 16 . . . cord-264359-m9j3pcj1 114 1 Simcation Simcation NNP cord-264359-m9j3pcj1 114 2 and and CC cord-264359-m9j3pcj1 114 3 finally finally RB cord-264359-m9j3pcj1 114 4 reextracted reextracte VBD cord-264359-m9j3pcj1 114 5 and and CC cord-264359-m9j3pcj1 114 6 reelectroilarly reelectroilarly RB cord-264359-m9j3pcj1 114 7 , , , cord-264359-m9j3pcj1 114 8 C3 C3 NNP cord-264359-m9j3pcj1 114 9 appears appear VBZ cord-264359-m9j3pcj1 114 10 to to TO cord-264359-m9j3pcj1 114 11 be be VB cord-264359-m9j3pcj1 114 12 derived derive VBN cord-264359-m9j3pcj1 114 13 from from IN cord-264359-m9j3pcj1 114 14 VP2 vp2 NN cord-264359-m9j3pcj1 114 15 , , , cord-264359-m9j3pcj1 114 16 phoresed phorese VBN cord-264359-m9j3pcj1 114 17 in in IN cord-264359-m9j3pcj1 114 18 the the DT cord-264359-m9j3pcj1 114 19 normal normal JJ cord-264359-m9j3pcj1 114 20 system system NN cord-264359-m9j3pcj1 114 21 . . . cord-264359-m9j3pcj1 115 1 The the DT cord-264359-m9j3pcj1 115 2 results result NNS cord-264359-m9j3pcj1 115 3 and and CC cord-264359-m9j3pcj1 115 4 both both CC cord-264359-m9j3pcj1 115 5 C5 C5 NNP cord-264359-m9j3pcj1 115 6 and and CC cord-264359-m9j3pcj1 115 7 C6 C6 NNP cord-264359-m9j3pcj1 115 8 appear appear VBP cord-264359-m9j3pcj1 115 9 to to TO cord-264359-m9j3pcj1 115 10 be be VB cord-264359-m9j3pcj1 115 11 derived derive VBN cord-264359-m9j3pcj1 115 12 ( ( -LRB- cord-264359-m9j3pcj1 115 13 not not RB cord-264359-m9j3pcj1 115 14 shown show VBN cord-264359-m9j3pcj1 115 15 ) ) -RRB- cord-264359-m9j3pcj1 115 16 can can MD cord-264359-m9j3pcj1 115 17 be be VB cord-264359-m9j3pcj1 115 18 summarized summarize VBN cord-264359-m9j3pcj1 115 19 as as IN cord-264359-m9j3pcj1 115 20 follows follow VBZ cord-264359-m9j3pcj1 115 21 : : : cord-264359-m9j3pcj1 115 22 from from IN cord-264359-m9j3pcj1 115 23 VP3 VP3 NNP cord-264359-m9j3pcj1 115 24 . . . cord-264359-m9j3pcj1 116 1 The the DT cord-264359-m9j3pcj1 116 2 results result NNS cord-264359-m9j3pcj1 116 3 concerning concern VBG cord-264359-m9j3pcj1 116 4 C2 C2 NNP cord-264359-m9j3pcj1 116 5 and and CC cord-264359-m9j3pcj1 116 6 Cl Cl NNP cord-264359-m9j3pcj1 116 7 on on IN cord-264359-m9j3pcj1 116 8 normal normal JJ cord-264359-m9j3pcj1 116 9 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 116 10 comigrated comigrate VBD cord-264359-m9j3pcj1 116 11 C4 C4 NNP cord-264359-m9j3pcj1 116 12 remained remain VBD cord-264359-m9j3pcj1 116 13 inconclusive inconclusive JJ cord-264359-m9j3pcj1 116 14 . . . cord-264359-m9j3pcj1 117 1 It -PRON- PRP cord-264359-m9j3pcj1 117 2 may may MD cord-264359-m9j3pcj1 117 3 be be VB cord-264359-m9j3pcj1 117 4 rewith rewith NNP cord-264359-m9j3pcj1 117 5 VPl VPl NNP cord-264359-m9j3pcj1 117 6 , , , cord-264359-m9j3pcj1 117 7 C3 C3 NNP cord-264359-m9j3pcj1 117 8 with with IN cord-264359-m9j3pcj1 117 9 VP2 vp2 NN cord-264359-m9j3pcj1 117 10 , , , cord-264359-m9j3pcj1 117 11 and and CC cord-264359-m9j3pcj1 117 12 both both DT cord-264359-m9j3pcj1 117 13 C5 C5 NNP cord-264359-m9j3pcj1 117 14 and and CC cord-264359-m9j3pcj1 117 15 C6 C6 NNP cord-264359-m9j3pcj1 117 16 with with IN cord-264359-m9j3pcj1 117 17 VP3 VP3 NNP cord-264359-m9j3pcj1 117 18 ( ( -LRB- cord-264359-m9j3pcj1 117 19 due due IN cord-264359-m9j3pcj1 117 20 to to IN cord-264359-m9j3pcj1 117 21 insufficient insufficient JJ cord-264359-m9j3pcj1 117 22 radioactiv radioactiv RB cord-264359-m9j3pcj1 117 23 - - HYPH cord-264359-m9j3pcj1 117 24 called call VBN cord-264359-m9j3pcj1 117 25 , , , cord-264359-m9j3pcj1 117 26 however however RB cord-264359-m9j3pcj1 117 27 , , , cord-264359-m9j3pcj1 117 28 that that IN cord-264359-m9j3pcj1 117 29 procapsids procapsid NNS cord-264359-m9j3pcj1 117 30 having have VBG cord-264359-m9j3pcj1 117 31 VP1 VP1 NNP cord-264359-m9j3pcj1 117 32 and and CC cord-264359-m9j3pcj1 117 33 lacking lack VBG cord-264359-m9j3pcj1 117 34 VP2 vp2 NN cord-264359-m9j3pcj1 117 35 yielded yield VBD cord-264359-m9j3pcj1 117 36 C2 C2 NNP cord-264359-m9j3pcj1 117 37 but but CC cord-264359-m9j3pcj1 117 38 neither neither CC cord-264359-m9j3pcj1 117 39 C3 c3 NN cord-264359-m9j3pcj1 117 40 nor nor CC cord-264359-m9j3pcj1 117 41 C4 c4 NN cord-264359-m9j3pcj1 117 42 ( ( -LRB- cord-264359-m9j3pcj1 117 43 Fig Fig NNP cord-264359-m9j3pcj1 117 44 . . . cord-264359-m9j3pcj1 117 45 3 3 CD cord-264359-m9j3pcj1 117 46 ) ) -RRB- cord-264359-m9j3pcj1 117 47 , , , cord-264359-m9j3pcj1 117 48 and and CC cord-264359-m9j3pcj1 117 49 this this DT cord-264359-m9j3pcj1 117 50 suggests suggest VBZ cord-264359-m9j3pcj1 117 51 that that IN cord-264359-m9j3pcj1 117 52 C2 C2 NNP cord-264359-m9j3pcj1 117 53 is be VBZ cord-264359-m9j3pcj1 117 54 derived derive VBN cord-264359-m9j3pcj1 117 55 from from IN cord-264359-m9j3pcj1 117 56 VP1 VP1 NNP cord-264359-m9j3pcj1 117 57 and and CC cord-264359-m9j3pcj1 117 58 C4 C4 NNP cord-264359-m9j3pcj1 117 59 from from IN cord-264359-m9j3pcj1 117 60 VP2 vp2 NN cord-264359-m9j3pcj1 117 61 . . . cord-264359-m9j3pcj1 118 1 The the DT cord-264359-m9j3pcj1 118 2 combined combine VBN cord-264359-m9j3pcj1 118 3 evidence evidence NN cord-264359-m9j3pcj1 118 4 yields yield VBZ cord-264359-m9j3pcj1 118 5 three three CD cord-264359-m9j3pcj1 118 6 tentative tentative JJ cord-264359-m9j3pcj1 118 7 doublets doublet NNS cord-264359-m9j3pcj1 118 8 : : : cord-264359-m9j3pcj1 118 9 VP1 VP1 NNP cord-264359-m9j3pcj1 118 10 = = NFP cord-264359-m9j3pcj1 119 1 Cl Cl NNP cord-264359-m9j3pcj1 119 2 + + CC cord-264359-m9j3pcj1 119 3 C2 C2 NNP cord-264359-m9j3pcj1 119 4 , , , cord-264359-m9j3pcj1 119 5 VP2 vp2 NN cord-264359-m9j3pcj1 119 6 = = SYM cord-264359-m9j3pcj1 119 7 C3 c3 NN cord-264359-m9j3pcj1 119 8 + + CC cord-264359-m9j3pcj1 119 9 C4 c4 NN cord-264359-m9j3pcj1 119 10 , , , cord-264359-m9j3pcj1 119 11 and and CC cord-264359-m9j3pcj1 119 12 VP3 VP3 NNP cord-264359-m9j3pcj1 119 13 = = SYM cord-264359-m9j3pcj1 119 14 C5 C5 NNP cord-264359-m9j3pcj1 119 15 + + CC cord-264359-m9j3pcj1 119 16 C6 c6 NN cord-264359-m9j3pcj1 119 17 . . . cord-264359-m9j3pcj1 120 1 Genetic genetic JJ cord-264359-m9j3pcj1 120 2 Relations Relations NNPS cord-264359-m9j3pcj1 120 3 Between between IN cord-264359-m9j3pcj1 120 4 Doublet Doublet NNP cord-264359-m9j3pcj1 120 5 Polypeptides Polypeptides NNP cord-264359-m9j3pcj1 120 6 Rekosh Rekosh NNP cord-264359-m9j3pcj1 120 7 ( ( -LRB- cord-264359-m9j3pcj1 120 8 1972 1972 CD cord-264359-m9j3pcj1 120 9 ) ) -RRB- cord-264359-m9j3pcj1 120 10 used use VBD cord-264359-m9j3pcj1 120 11 the the DT cord-264359-m9j3pcj1 120 12 labeling labeling NN cord-264359-m9j3pcj1 120 13 kinetics kinetic NNS cord-264359-m9j3pcj1 120 14 of of IN cord-264359-m9j3pcj1 120 15 the the DT cord-264359-m9j3pcj1 120 16 poliovirus poliovirus NN cord-264359-m9j3pcj1 120 17 capsid capsid NN cord-264359-m9j3pcj1 120 18 polypeptides polypeptide NNS cord-264359-m9j3pcj1 120 19 in in IN cord-264359-m9j3pcj1 120 20 emetine emetine NN cord-264359-m9j3pcj1 120 21 - - HYPH cord-264359-m9j3pcj1 120 22 stopped stop VBN cord-264359-m9j3pcj1 120 23 pulses pulse NNS cord-264359-m9j3pcj1 120 24 of of IN cord-264359-m9j3pcj1 120 25 r3H]leucine r3h]leucine NN cord-264359-m9j3pcj1 120 26 incorporation incorporation NN cord-264359-m9j3pcj1 120 27 to to TO cord-264359-m9j3pcj1 120 28 determine determine VB cord-264359-m9j3pcj1 120 29 the the DT cord-264359-m9j3pcj1 120 30 order order NN cord-264359-m9j3pcj1 120 31 of of IN cord-264359-m9j3pcj1 120 32 the the DT cord-264359-m9j3pcj1 120 33 RNA RNA NNP cord-264359-m9j3pcj1 120 34 regions region NNS cord-264359-m9j3pcj1 120 35 encoding encode VBG cord-264359-m9j3pcj1 120 36 the the DT cord-264359-m9j3pcj1 120 37 polypeptides polypeptide NNS cord-264359-m9j3pcj1 120 38 . . . cord-264359-m9j3pcj1 121 1 As as IN cord-264359-m9j3pcj1 121 2 the the DT cord-264359-m9j3pcj1 121 3 length length NN cord-264359-m9j3pcj1 121 4 of of IN cord-264359-m9j3pcj1 121 5 the the DT cord-264359-m9j3pcj1 121 6 pulses pulse NNS cord-264359-m9j3pcj1 121 7 was be VBD cord-264359-m9j3pcj1 121 8 increased increase VBN cord-264359-m9j3pcj1 121 9 , , , cord-264359-m9j3pcj1 121 10 VPl VPl NNP cord-264359-m9j3pcj1 121 11 , , , cord-264359-m9j3pcj1 121 12 VP3 VP3 NNP cord-264359-m9j3pcj1 121 13 , , , cord-264359-m9j3pcj1 121 14 and and CC cord-264359-m9j3pcj1 121 15 VP2 vp2 NN cord-264359-m9j3pcj1 121 16 became become VBD cord-264359-m9j3pcj1 121 17 labeled label VBN cord-264359-m9j3pcj1 121 18 in in IN cord-264359-m9j3pcj1 121 19 turn turn NN cord-264359-m9j3pcj1 121 20 . . . cord-264359-m9j3pcj1 122 1 The the DT cord-264359-m9j3pcj1 122 2 Rekosh Rekosh NNP cord-264359-m9j3pcj1 122 3 experiment experiment NN cord-264359-m9j3pcj1 122 4 was be VBD cord-264359-m9j3pcj1 122 5 easily easily RB cord-264359-m9j3pcj1 122 6 reproduced reproduce VBN cord-264359-m9j3pcj1 122 7 using use VBG cord-264359-m9j3pcj1 122 8 our -PRON- PRP$ cord-264359-m9j3pcj1 122 9 la la NNP cord-264359-m9j3pcj1 122 10 / / SYM cord-264359-m9j3pcj1 122 11 S3 S3 NNP cord-264359-m9j3pcj1 122 12 strain strain NN cord-264359-m9j3pcj1 122 13 and and CC cord-264359-m9j3pcj1 122 14 the the DT cord-264359-m9j3pcj1 122 15 normal normal JJ cord-264359-m9j3pcj1 122 16 electrophoretic electrophoretic JJ cord-264359-m9j3pcj1 122 17 technique technique NN cord-264359-m9j3pcj1 122 18 to to IN cord-264359-m9j3pcj1 122 19 separate separate JJ cord-264359-m9j3pcj1 122 20 VPl VPl NNS cord-264359-m9j3pcj1 122 21 , , , cord-264359-m9j3pcj1 122 22 VP2 vp2 NN cord-264359-m9j3pcj1 122 23 , , , cord-264359-m9j3pcj1 122 24 and and CC cord-264359-m9j3pcj1 122 25 VP3 VP3 NNP cord-264359-m9j3pcj1 122 26 ( ( -LRB- cord-264359-m9j3pcj1 122 27 not not RB cord-264359-m9j3pcj1 122 28 shown show VBN cord-264359-m9j3pcj1 122 29 ) ) -RRB- cord-264359-m9j3pcj1 122 30 . . . cord-264359-m9j3pcj1 123 1 The the DT cord-264359-m9j3pcj1 123 2 ex ex NN cord-264359-m9j3pcj1 123 3 - - NN cord-264359-m9j3pcj1 123 4 periment periment NN cord-264359-m9j3pcj1 123 5 was be VBD cord-264359-m9j3pcj1 123 6 repeated repeat VBN cord-264359-m9j3pcj1 123 7 using use VBG cord-264359-m9j3pcj1 123 8 the the DT cord-264359-m9j3pcj1 123 9 pH pH NNP cord-264359-m9j3pcj1 123 10 gradient gradient NN cord-264359-m9j3pcj1 123 11 technique technique NN cord-264359-m9j3pcj1 123 12 , , , cord-264359-m9j3pcj1 123 13 and and CC cord-264359-m9j3pcj1 123 14 the the DT cord-264359-m9j3pcj1 123 15 most most RBS cord-264359-m9j3pcj1 123 16 relevant relevant JJ cord-264359-m9j3pcj1 123 17 results result NNS cord-264359-m9j3pcj1 123 18 are be VBP cord-264359-m9j3pcj1 123 19 shown show VBN cord-264359-m9j3pcj1 123 20 in in IN cord-264359-m9j3pcj1 123 21 Fig Fig NNP cord-264359-m9j3pcj1 123 22 . . . cord-264359-m9j3pcj1 124 1 6 6 LS cord-264359-m9j3pcj1 124 2 . . . cord-264359-m9j3pcj1 125 1 Cl Cl NNP cord-264359-m9j3pcj1 125 2 was be VBD cord-264359-m9j3pcj1 125 3 the the DT cord-264359-m9j3pcj1 125 4 first first JJ cord-264359-m9j3pcj1 125 5 component component NN cord-264359-m9j3pcj1 125 6 into into IN cord-264359-m9j3pcj1 125 7 which which WDT cord-264359-m9j3pcj1 125 8 [ [ -LRB- cord-264359-m9j3pcj1 125 9 3H 3H NNP cord-264359-m9j3pcj1 125 10 ] ] -RRB- cord-264359-m9j3pcj1 125 11 leucine leucine NN cord-264359-m9j3pcj1 125 12 was be VBD cord-264359-m9j3pcj1 125 13 incorporated incorporate VBN cord-264359-m9j3pcj1 125 14 ( ( -LRB- cord-264359-m9j3pcj1 125 15 Fig Fig NNP cord-264359-m9j3pcj1 125 16 . . . cord-264359-m9j3pcj1 125 17 6A 6a CD cord-264359-m9j3pcj1 125 18 ) ) -RRB- cord-264359-m9j3pcj1 125 19 , , , cord-264359-m9j3pcj1 125 20 and and CC cord-264359-m9j3pcj1 125 21 the the DT cord-264359-m9j3pcj1 125 22 next next JJ cord-264359-m9j3pcj1 125 23 was be VBD cord-264359-m9j3pcj1 125 24 C6 C6 NNP cord-264359-m9j3pcj1 125 25 ( ( -LRB- cord-264359-m9j3pcj1 125 26 this this DT cord-264359-m9j3pcj1 125 27 component component NN cord-264359-m9j3pcj1 125 28 was be VBD cord-264359-m9j3pcj1 125 29 exceptionally exceptionally RB cord-264359-m9j3pcj1 125 30 represented represent VBN cord-264359-m9j3pcj1 125 31 by by IN cord-264359-m9j3pcj1 125 32 twin twin JJ cord-264359-m9j3pcj1 125 33 peaks peak NNS cord-264359-m9j3pcj1 125 34 ) ) -RRB- cord-264359-m9j3pcj1 125 35 . . . cord-264359-m9j3pcj1 126 1 After after IN cord-264359-m9j3pcj1 126 2 3 3 CD cord-264359-m9j3pcj1 126 3 min min NN cord-264359-m9j3pcj1 126 4 , , , cord-264359-m9j3pcj1 126 5 there there EX cord-264359-m9j3pcj1 126 6 was be VBD cord-264359-m9j3pcj1 126 7 no no DT cord-264359-m9j3pcj1 126 8 detectable detectable JJ cord-264359-m9j3pcj1 126 9 " " `` cord-264359-m9j3pcj1 126 10 H h NN cord-264359-m9j3pcj1 126 11 radioactivity radioactivity NN cord-264359-m9j3pcj1 126 12 in in IN cord-264359-m9j3pcj1 126 13 the the DT cord-264359-m9j3pcj1 126 14 C3 c3 NN cord-264359-m9j3pcj1 126 15 to to IN cord-264359-m9j3pcj1 126 16 C4 C4 NNP cord-264359-m9j3pcj1 126 17 area area NN cord-264359-m9j3pcj1 127 1 ( ( -LRB- cord-264359-m9j3pcj1 127 2 Fig Fig NNP cord-264359-m9j3pcj1 127 3 . . . cord-264359-m9j3pcj1 127 4 6A 6a CD cord-264359-m9j3pcj1 127 5 ) ) -RRB- cord-264359-m9j3pcj1 127 6 , , . cord-264359-m9j3pcj1 128 1 but but CC cord-264359-m9j3pcj1 128 2 these these DT cord-264359-m9j3pcj1 128 3 components component NNS cord-264359-m9j3pcj1 128 4 became become VBD cord-264359-m9j3pcj1 128 5 progressively progressively RB cord-264359-m9j3pcj1 128 6 labeled label VBN cord-264359-m9j3pcj1 128 7 as as IN cord-264359-m9j3pcj1 128 8 pulse pulse NN cord-264359-m9j3pcj1 128 9 length length NN cord-264359-m9j3pcj1 128 10 increased increase VBN cord-264359-m9j3pcj1 128 11 ( ( -LRB- cord-264359-m9j3pcj1 128 12 Figs fig NNS cord-264359-m9j3pcj1 128 13 . . . cord-264359-m9j3pcj1 129 1 6B-6D 6b-6d LS cord-264359-m9j3pcj1 129 2 ) ) -RRB- cord-264359-m9j3pcj1 129 3 . . . cord-264359-m9j3pcj1 130 1 The the DT cord-264359-m9j3pcj1 130 2 data datum NNS cord-264359-m9j3pcj1 130 3 did do VBD cord-264359-m9j3pcj1 130 4 not not RB cord-264359-m9j3pcj1 130 5 allow allow VB cord-264359-m9j3pcj1 130 6 any any DT cord-264359-m9j3pcj1 130 7 conclusion conclusion NN cord-264359-m9j3pcj1 130 8 about about IN cord-264359-m9j3pcj1 130 9 C2 C2 NNP cord-264359-m9j3pcj1 130 10 , , , cord-264359-m9j3pcj1 130 11 which which WDT cord-264359-m9j3pcj1 130 12 was be VBD cord-264359-m9j3pcj1 130 13 present present JJ cord-264359-m9j3pcj1 130 14 only only RB cord-264359-m9j3pcj1 130 15 in in IN cord-264359-m9j3pcj1 130 16 trace trace NN cord-264359-m9j3pcj1 130 17 amounts amount NNS cord-264359-m9j3pcj1 130 18 , , , cord-264359-m9j3pcj1 130 19 nor nor CC cord-264359-m9j3pcj1 130 20 about about IN cord-264359-m9j3pcj1 130 21 C5 C5 NNP cord-264359-m9j3pcj1 130 22 , , , cord-264359-m9j3pcj1 130 23 which which WDT cord-264359-m9j3pcj1 130 24 appeared appear VBD cord-264359-m9j3pcj1 130 25 as as IN cord-264359-m9j3pcj1 130 26 a a DT cord-264359-m9j3pcj1 130 27 shoulder shoulder NN cord-264359-m9j3pcj1 130 28 in in IN cord-264359-m9j3pcj1 130 29 some some DT cord-264359-m9j3pcj1 130 30 of of IN cord-264359-m9j3pcj1 130 31 the the DT cord-264359-m9j3pcj1 130 32 3H 3h CD cord-264359-m9j3pcj1 130 33 - - HYPH cord-264359-m9j3pcj1 130 34 profiles profile NNS cord-264359-m9j3pcj1 130 35 ( ( -LRB- cord-264359-m9j3pcj1 130 36 Figs fig NNS cord-264359-m9j3pcj1 130 37 . . . cord-264359-m9j3pcj1 131 1 6A 6A NNP cord-264359-m9j3pcj1 131 2 and and CC cord-264359-m9j3pcj1 131 3 6B 6b CD cord-264359-m9j3pcj1 131 4 ) ) -RRB- cord-264359-m9j3pcj1 131 5 . . . cord-264359-m9j3pcj1 132 1 To to TO cord-264359-m9j3pcj1 132 2 determine determine VB cord-264359-m9j3pcj1 132 3 the the DT cord-264359-m9j3pcj1 132 4 3H/'4C 3h/'4c CD cord-264359-m9j3pcj1 132 5 ratio ratio NN cord-264359-m9j3pcj1 132 6 for for IN cord-264359-m9j3pcj1 132 7 C5 C5 NNP cord-264359-m9j3pcj1 132 8 , , , cord-264359-m9j3pcj1 132 9 this this DT cord-264359-m9j3pcj1 132 10 component component NN cord-264359-m9j3pcj1 132 11 was be VBD cord-264359-m9j3pcj1 132 12 further further RB cord-264359-m9j3pcj1 132 13 purified purify VBN cord-264359-m9j3pcj1 132 14 by by IN cord-264359-m9j3pcj1 132 15 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 132 16 . . . cord-264359-m9j3pcj1 133 1 To to IN cord-264359-m9j3pcj1 133 2 this this DT cord-264359-m9j3pcj1 133 3 end end NN cord-264359-m9j3pcj1 133 4 , , , cord-264359-m9j3pcj1 133 5 the the DT cord-264359-m9j3pcj1 133 6 relevant relevant JJ cord-264359-m9j3pcj1 133 7 fractions fraction NNS cord-264359-m9j3pcj1 133 8 ( ( -LRB- cord-264359-m9j3pcj1 133 9 Figs fig NNS cord-264359-m9j3pcj1 133 10 . . . cord-264359-m9j3pcj1 133 11 6B-6D 6b-6d CD cord-264359-m9j3pcj1 133 12 ) ) -RRB- cord-264359-m9j3pcj1 133 13 were be VBD cord-264359-m9j3pcj1 133 14 pooled pool VBN cord-264359-m9j3pcj1 133 15 , , , cord-264359-m9j3pcj1 133 16 con- con- NN cord-264359-m9j3pcj1 133 17 : : : cord-264359-m9j3pcj1 133 18 ' ' '' cord-264359-m9j3pcj1 133 19 , , , cord-264359-m9j3pcj1 133 20 -centrated -centrated JJ cord-264359-m9j3pcj1 133 21 , , , cord-264359-m9j3pcj1 133 22 reextracted reextracted JJ cord-264359-m9j3pcj1 133 23 , , , cord-264359-m9j3pcj1 133 24 and and CC cord-264359-m9j3pcj1 133 25 reelectrophoresed reelectrophorese VBD cord-264359-m9j3pcj1 133 26 as as IN cord-264359-m9j3pcj1 133 27 before before RB cord-264359-m9j3pcj1 133 28 ( ( -LRB- cord-264359-m9j3pcj1 133 29 pH pH NNP cord-264359-m9j3pcj1 133 30 gradient gradient NN cord-264359-m9j3pcj1 133 31 technique technique NN cord-264359-m9j3pcj1 133 32 , , , cord-264359-m9j3pcj1 133 33 38 38 CD cord-264359-m9j3pcj1 133 34 hr hr NN cord-264359-m9j3pcj1 133 35 ) ) -RRB- cord-264359-m9j3pcj1 133 36 . . . cord-264359-m9j3pcj1 134 1 For for IN cord-264359-m9j3pcj1 134 2 purposes purpose NNS cord-264359-m9j3pcj1 134 3 of of IN cord-264359-m9j3pcj1 134 4 comparison comparison NN cord-264359-m9j3pcj1 134 5 , , , cord-264359-m9j3pcj1 134 6 the the DT cord-264359-m9j3pcj1 134 7 same same JJ cord-264359-m9j3pcj1 134 8 procedure procedure NN cord-264359-m9j3pcj1 134 9 was be VBD cord-264359-m9j3pcj1 134 10 also also RB cord-264359-m9j3pcj1 134 11 applied apply VBN cord-264359-m9j3pcj1 134 12 to to IN cord-264359-m9j3pcj1 134 13 pools pool NNS cord-264359-m9j3pcj1 134 14 of of IN cord-264359-m9j3pcj1 134 15 fractions fraction NNS cord-264359-m9j3pcj1 134 16 representing represent VBG cord-264359-m9j3pcj1 134 17 Cl Cl NNP cord-264359-m9j3pcj1 134 18 , , , cord-264359-m9j3pcj1 134 19 the the DT cord-264359-m9j3pcj1 134 20 C3-C4 C3-C4 NNP cord-264359-m9j3pcj1 134 21 area area NN cord-264359-m9j3pcj1 134 22 , , , cord-264359-m9j3pcj1 134 23 and and CC cord-264359-m9j3pcj1 134 24 C6 C6 NNP cord-264359-m9j3pcj1 134 25 . . . cord-264359-m9j3pcj1 135 1 The the DT cord-264359-m9j3pcj1 135 2 3H/'4C 3h/'4c CD cord-264359-m9j3pcj1 135 3 ratios ratio NNS cord-264359-m9j3pcj1 135 4 were be VBD cord-264359-m9j3pcj1 135 5 computed compute VBN cord-264359-m9j3pcj1 135 6 from from IN cord-264359-m9j3pcj1 135 7 the the DT cord-264359-m9j3pcj1 135 8 radioactivity radioactivity NN cord-264359-m9j3pcj1 135 9 profiles profile NNS cord-264359-m9j3pcj1 135 10 ( ( -LRB- cord-264359-m9j3pcj1 135 11 not not RB cord-264359-m9j3pcj1 135 12 shown show VBN cord-264359-m9j3pcj1 135 13 ) ) -RRB- cord-264359-m9j3pcj1 135 14 , , , cord-264359-m9j3pcj1 135 15 normalized normalize VBN cord-264359-m9j3pcj1 135 16 , , , cord-264359-m9j3pcj1 135 17 and and CC cord-264359-m9j3pcj1 135 18 plotted plot VBN cord-264359-m9j3pcj1 135 19 against against IN cord-264359-m9j3pcj1 135 20 the the DT cord-264359-m9j3pcj1 135 21 duration duration NN cord-264359-m9j3pcj1 135 22 of of IN cord-264359-m9j3pcj1 135 23 the the DT cord-264359-m9j3pcj1 135 24 pulse pulse NN cord-264359-m9j3pcj1 136 1 ( ( -LRB- cord-264359-m9j3pcj1 136 2 Fig Fig NNP cord-264359-m9j3pcj1 136 3 . . NNP cord-264359-m9j3pcj1 136 4 7 7 CD cord-264359-m9j3pcj1 136 5 ) ) -RRB- cord-264359-m9j3pcj1 136 6 . . . cord-264359-m9j3pcj1 137 1 The the DT cord-264359-m9j3pcj1 137 2 data datum NNS cord-264359-m9j3pcj1 137 3 show show VBP cord-264359-m9j3pcj1 137 4 the the DT cord-264359-m9j3pcj1 137 5 existence existence NN cord-264359-m9j3pcj1 137 6 of of IN cord-264359-m9j3pcj1 137 7 three three CD cord-264359-m9j3pcj1 137 8 different different JJ cord-264359-m9j3pcj1 137 9 labeling labeling NN cord-264359-m9j3pcj1 137 10 kinetics kinetic NNS cord-264359-m9j3pcj1 137 11 represented represent VBN cord-264359-m9j3pcj1 137 12 by by IN cord-264359-m9j3pcj1 137 13 Cl Cl NNP cord-264359-m9j3pcj1 137 14 , , , cord-264359-m9j3pcj1 137 15 and and CC cord-264359-m9j3pcj1 137 16 C5-C6 C5-C6 NNP cord-264359-m9j3pcj1 137 17 group group NN cord-264359-m9j3pcj1 137 18 , , , cord-264359-m9j3pcj1 137 19 and and CC cord-264359-m9j3pcj1 137 20 The the DT cord-264359-m9j3pcj1 137 21 _SP cord-264359-m9j3pcj1 137 22 order order NN cord-264359-m9j3pcj1 137 23 _SP cord-264359-m9j3pcj1 137 24 of of IN cord-264359-m9j3pcj1 137 25 _SP cord-264359-m9j3pcj1 137 26 labeling label VBG cord-264359-m9j3pcj1 137 27 _SP cord-264359-m9j3pcj1 137 28 was be VBD cord-264359-m9j3pcj1 137 29 _SP cord-264359-m9j3pcj1 137 30 VPl VPl NNP cord-264359-m9j3pcj1 137 31 - - HYPH cord-264359-m9j3pcj1 137 32 VP3-VP2 VP3-VP2 NNP cord-264359-m9j3pcj1 137 33 or or CC cord-264359-m9j3pcj1 137 34 ( ( -LRB- cord-264359-m9j3pcj1 137 35 Cl Cl NNP cord-264359-m9j3pcj1 137 36 , , , cord-264359-m9j3pcj1 137 37 C2)-(C5 C2)-(C5 NNP cord-264359-m9j3pcj1 137 38 , , , cord-264359-m9j3pcj1 137 39 C6)-(C3 c6)-(c3 NN cord-264359-m9j3pcj1 137 40 , , , cord-264359-m9j3pcj1 137 41 C4 C4 NNP cord-264359-m9j3pcj1 137 42 ) ) -RRB- cord-264359-m9j3pcj1 137 43 , , , cord-264359-m9j3pcj1 137 44 according accord VBG cord-264359-m9j3pcj1 137 45 to to IN cord-264359-m9j3pcj1 137 46 whether whether IN cord-264359-m9j3pcj1 137 47 the the DT cord-264359-m9j3pcj1 137 48 polypeptides polypeptide NNS cord-264359-m9j3pcj1 137 49 were be VBD cord-264359-m9j3pcj1 137 50 analyzed analyze VBN cord-264359-m9j3pcj1 137 51 by by IN cord-264359-m9j3pcj1 137 52 normal normal JJ cord-264359-m9j3pcj1 137 53 or or CC cord-264359-m9j3pcj1 137 54 by by IN cord-264359-m9j3pcj1 137 55 pH pH NNP cord-264359-m9j3pcj1 137 56 gradient gradient NN cord-264359-m9j3pcj1 137 57 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 137 58 . . . cord-264359-m9j3pcj1 138 1 Thus thus RB cord-264359-m9j3pcj1 138 2 , , , cord-264359-m9j3pcj1 138 3 the the DT cord-264359-m9j3pcj1 138 4 six six CD cord-264359-m9j3pcj1 138 5 electrophoretie electrophoretie NN cord-264359-m9j3pcj1 138 6 components component NNS cord-264359-m9j3pcj1 138 7 , , , cord-264359-m9j3pcj1 138 8 Cl Cl NNP cord-264359-m9j3pcj1 138 9 to to IN cord-264359-m9j3pcj1 138 10 C6 C6 NNP cord-264359-m9j3pcj1 138 11 , , , cord-264359-m9j3pcj1 138 12 resolved resolve VBN cord-264359-m9j3pcj1 138 13 in in IN cord-264359-m9j3pcj1 138 14 pH pH NNP cord-264359-m9j3pcj1 138 15 gradient gradient NN cord-264359-m9j3pcj1 138 16 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 138 17 could could MD cord-264359-m9j3pcj1 138 18 be be VB cord-264359-m9j3pcj1 138 19 attributed attribute VBN cord-264359-m9j3pcj1 138 20 to to IN cord-264359-m9j3pcj1 138 21 three three CD cord-264359-m9j3pcj1 138 22 doublets doublet NNS cord-264359-m9j3pcj1 138 23 according accord VBG cord-264359-m9j3pcj1 138 24 to to IN cord-264359-m9j3pcj1 138 25 their -PRON- PRP$ cord-264359-m9j3pcj1 138 26 location location NN cord-264359-m9j3pcj1 138 27 on on IN cord-264359-m9j3pcj1 138 28 the the DT cord-264359-m9j3pcj1 138 29 viral viral JJ cord-264359-m9j3pcj1 138 30 RNA RNA NNP cord-264359-m9j3pcj1 138 31 , , , cord-264359-m9j3pcj1 138 32 and and CC cord-264359-m9j3pcj1 138 33 these these DT cord-264359-m9j3pcj1 138 34 doublets doublet NNS cord-264359-m9j3pcj1 138 35 were be VBD cord-264359-m9j3pcj1 138 36 the the DT cord-264359-m9j3pcj1 138 37 same same JJ cord-264359-m9j3pcj1 138 38 that that WDT cord-264359-m9j3pcj1 138 39 were be VBD cord-264359-m9j3pcj1 138 40 independently independently RB cord-264359-m9j3pcj1 138 41 proposed propose VBN cord-264359-m9j3pcj1 138 42 above above RB cord-264359-m9j3pcj1 138 43 as as IN cord-264359-m9j3pcj1 138 44 the the DT cord-264359-m9j3pcj1 138 45 tentative tentative JJ cord-264359-m9j3pcj1 138 46 conclusion conclusion NN cord-264359-m9j3pcj1 138 47 of of IN cord-264359-m9j3pcj1 138 48 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 138 49 experiments experiment NNS cord-264359-m9j3pcj1 138 50 . . . cord-264359-m9j3pcj1 139 1 In in IN cord-264359-m9j3pcj1 139 2 this this DT cord-264359-m9j3pcj1 139 3 experiment experiment NN cord-264359-m9j3pcj1 139 4 , , , cord-264359-m9j3pcj1 139 5 la la NNP cord-264359-m9j3pcj1 139 6 / / SYM cord-264359-m9j3pcj1 139 7 S3 S3 NNP cord-264359-m9j3pcj1 139 8 virus virus NN cord-264359-m9j3pcj1 139 9 of of IN cord-264359-m9j3pcj1 139 10 sufhcient sufhcient JJ cord-264359-m9j3pcj1 139 11 radioactivity radioactivity NN cord-264359-m9j3pcj1 139 12 was be VBD cord-264359-m9j3pcj1 139 13 used use VBN cord-264359-m9j3pcj1 139 14 to to TO cord-264359-m9j3pcj1 139 15 allow allow VB cord-264359-m9j3pcj1 139 16 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 139 17 of of IN cord-264359-m9j3pcj1 139 18 single single JJ cord-264359-m9j3pcj1 139 19 - - HYPH cord-264359-m9j3pcj1 139 20 slice slice NN cord-264359-m9j3pcj1 139 21 eluates eluate NNS cord-264359-m9j3pcj1 139 22 ( ( -LRB- cord-264359-m9j3pcj1 139 23 Fig Fig NNP cord-264359-m9j3pcj1 139 24 . . . cord-264359-m9j3pcj1 139 25 8) 8) NNP cord-264359-m9j3pcj1 139 26 . . . cord-264359-m9j3pcj1 140 1 The the DT cord-264359-m9j3pcj1 140 2 polypeptide polypeptide NN cord-264359-m9j3pcj1 140 3 pattern pattern NN cord-264359-m9j3pcj1 140 4 after after IN cord-264359-m9j3pcj1 140 5 pH pH NNP cord-264359-m9j3pcj1 140 6 gradient gradient NN cord-264359-m9j3pcj1 140 7 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 140 8 is be VBZ cord-264359-m9j3pcj1 140 9 shown show VBN cord-264359-m9j3pcj1 140 10 in in IN cord-264359-m9j3pcj1 140 11 Fig Fig NNP cord-264359-m9j3pcj1 140 12 . . . cord-264359-m9j3pcj1 141 1 80 80 CD cord-264359-m9j3pcj1 141 2 . . . cord-264359-m9j3pcj1 142 1 The the DT cord-264359-m9j3pcj1 142 2 eluates eluate NNS cord-264359-m9j3pcj1 142 3 of of IN cord-264359-m9j3pcj1 142 4 fifteen fifteen CD cord-264359-m9j3pcj1 142 5 different different JJ cord-264359-m9j3pcj1 142 6 slices slice NNS cord-264359-m9j3pcj1 142 7 were be VBD cord-264359-m9j3pcj1 142 8 each each DT cord-264359-m9j3pcj1 142 9 mixed mixed JJ cord-264359-m9j3pcj1 142 10 with with IN cord-264359-m9j3pcj1 142 11 14C 14c NN cord-264359-m9j3pcj1 142 12 - - HYPH cord-264359-m9j3pcj1 142 13 labeled label VBN cord-264359-m9j3pcj1 142 14 virus virus NN cord-264359-m9j3pcj1 142 15 and and CC cord-264359-m9j3pcj1 142 16 submitted submit VBD cord-264359-m9j3pcj1 142 17 again again RB cord-264359-m9j3pcj1 142 18 to to IN cord-264359-m9j3pcj1 142 19 an an DT cord-264359-m9j3pcj1 142 20 exact exact JJ cord-264359-m9j3pcj1 142 21 repetition repetition NN cord-264359-m9j3pcj1 142 22 of of IN cord-264359-m9j3pcj1 142 23 the the DT cord-264359-m9j3pcj1 142 24 original original JJ cord-264359-m9j3pcj1 142 25 protein protein NN cord-264359-m9j3pcj1 142 26 extraction extraction NN cord-264359-m9j3pcj1 142 27 procedure procedure NN cord-264359-m9j3pcj1 142 28 and and CC cord-264359-m9j3pcj1 142 29 to to IN cord-264359-m9j3pcj1 142 30 pH pH NNP cord-264359-m9j3pcj1 142 31 gradient gradient NN cord-264359-m9j3pcj1 142 32 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 142 33 . . . cord-264359-m9j3pcj1 143 1 The the DT cord-264359-m9j3pcj1 143 2 most most RBS cord-264359-m9j3pcj1 143 3 relevant relevant JJ cord-264359-m9j3pcj1 143 4 radioactivity radioactivity NN cord-264359-m9j3pcj1 143 5 profiles profile NNS cord-264359-m9j3pcj1 143 6 are be VBP cord-264359-m9j3pcj1 143 7 shown show VBN cord-264359-m9j3pcj1 143 8 in in IN cord-264359-m9j3pcj1 143 9 Figs Figs NNPS cord-264359-m9j3pcj1 143 10 . . . cord-264359-m9j3pcj1 144 1 8A 8A NNP cord-264359-m9j3pcj1 144 2 - - HYPH cord-264359-m9j3pcj1 144 3 BG BG NNP cord-264359-m9j3pcj1 144 4 , , , cord-264359-m9j3pcj1 144 5 and and CC cord-264359-m9j3pcj1 144 6 the the DT cord-264359-m9j3pcj1 144 7 results result NNS cord-264359-m9j3pcj1 144 8 can can MD cord-264359-m9j3pcj1 144 9 be be VB cord-264359-m9j3pcj1 144 10 summarized summarize VBN cord-264359-m9j3pcj1 144 11 as as IN cord-264359-m9j3pcj1 144 12 follows follow VBZ cord-264359-m9j3pcj1 144 13 : : : cord-264359-m9j3pcj1 145 1 ( ( -LRB- cord-264359-m9j3pcj1 145 2 i i NN cord-264359-m9j3pcj1 145 3 ) ) -RRB- cord-264359-m9j3pcj1 145 4 Material material NN cord-264359-m9j3pcj1 145 5 collected collect VBN cord-264359-m9j3pcj1 145 6 at at IN cord-264359-m9j3pcj1 145 7 or or CC cord-264359-m9j3pcj1 145 8 near near IN cord-264359-m9j3pcj1 145 9 the the DT cord-264359-m9j3pcj1 145 10 center center NN cord-264359-m9j3pcj1 145 11 of of IN cord-264359-m9j3pcj1 145 12 peaks peaks NNP cord-264359-m9j3pcj1 145 13 Cl Cl NNP cord-264359-m9j3pcj1 145 14 , , , cord-264359-m9j3pcj1 145 15 C5 C5 NNP cord-264359-m9j3pcj1 145 16 , , , cord-264359-m9j3pcj1 145 17 or or CC cord-264359-m9j3pcj1 145 18 C6 C6 NNP cord-264359-m9j3pcj1 145 19 upon upon IN cord-264359-m9j3pcj1 145 20 reelectrophoresis reelectrophoresis NNP cord-264359-m9j3pcj1 145 21 yielded yield VBD cord-264359-m9j3pcj1 145 22 a a DT cord-264359-m9j3pcj1 145 23 single single JJ cord-264359-m9j3pcj1 145 24 peak peak NN cord-264359-m9j3pcj1 145 25 ( ( -LRB- cord-264359-m9j3pcj1 145 26 Figs fig NNS cord-264359-m9j3pcj1 145 27 . . . cord-264359-m9j3pcj1 146 1 8A 8A NNP cord-264359-m9j3pcj1 146 2 , , , cord-264359-m9j3pcj1 146 3 BE BE NNP cord-264359-m9j3pcj1 146 4 , , , cord-264359-m9j3pcj1 146 5 and and CC cord-264359-m9j3pcj1 146 6 SC SC NNP cord-264359-m9j3pcj1 146 7 ) ) -RRB- cord-264359-m9j3pcj1 146 8 . . . cord-264359-m9j3pcj1 147 1 This this DT cord-264359-m9j3pcj1 147 2 shows show VBZ cord-264359-m9j3pcj1 147 3 the the DT cord-264359-m9j3pcj1 147 4 stability stability NN cord-264359-m9j3pcj1 147 5 of of IN cord-264359-m9j3pcj1 147 6 the the DT cord-264359-m9j3pcj1 147 7 protein protein NN cord-264359-m9j3pcj1 147 8 - - HYPH cord-264359-m9j3pcj1 147 9 SDS SDS NNP cord-264359-m9j3pcj1 147 10 complexes complex NNS cord-264359-m9j3pcj1 147 11 throughout throughout IN cord-264359-m9j3pcj1 147 12 the the DT cord-264359-m9j3pcj1 147 13 extraction extraction NN cord-264359-m9j3pcj1 147 14 procedure procedure NN cord-264359-m9j3pcj1 147 15 and and CC cord-264359-m9j3pcj1 147 16 the the DT cord-264359-m9j3pcj1 147 17 absence absence NN cord-264359-m9j3pcj1 147 18 of of IN cord-264359-m9j3pcj1 147 19 conversion conversion NN cord-264359-m9j3pcj1 147 20 to to IN cord-264359-m9j3pcj1 147 21 the the DT cord-264359-m9j3pcj1 147 22 doublet doublet NN cord-264359-m9j3pcj1 147 23 partner partner NN cord-264359-m9j3pcj1 147 24 ; ; , cord-264359-m9j3pcj1 147 25 ( ( -LRB- cord-264359-m9j3pcj1 147 26 ii ii NN cord-264359-m9j3pcj1 147 27 ) ) -RRB- cord-264359-m9j3pcj1 147 28 material material NN cord-264359-m9j3pcj1 147 29 taken take VBN cord-264359-m9j3pcj1 147 30 from from IN cord-264359-m9j3pcj1 147 31 the the DT cord-264359-m9j3pcj1 147 32 left left NN cord-264359-m9j3pcj1 147 33 , , , cord-264359-m9j3pcj1 147 34 center center NN cord-264359-m9j3pcj1 147 35 , , , cord-264359-m9j3pcj1 147 36 or or CC cord-264359-m9j3pcj1 147 37 right right NN cord-264359-m9j3pcj1 147 38 of of IN cord-264359-m9j3pcj1 147 39 peak peak NNP cord-264359-m9j3pcj1 147 40 Cl Cl NNP cord-264359-m9j3pcj1 147 41 migrated migrate VBD cord-264359-m9j3pcj1 147 42 identically identically RB cord-264359-m9j3pcj1 147 43 upon upon IN cord-264359-m9j3pcj1 147 44 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 147 45 ( ( -LRB- cord-264359-m9j3pcj1 147 46 results result NNS cord-264359-m9j3pcj1 147 47 partly partly RB cord-264359-m9j3pcj1 147 48 shown show VBN cord-264359-m9j3pcj1 147 49 in in IN cord-264359-m9j3pcj1 147 50 Figs Figs NNPS cord-264359-m9j3pcj1 147 51 . . . cord-264359-m9j3pcj1 148 1 BA BA NNP cord-264359-m9j3pcj1 148 2 - - HYPH cord-264359-m9j3pcj1 148 3 BB BB NNP cord-264359-m9j3pcj1 148 4 ) ) -RRB- cord-264359-m9j3pcj1 148 5 , , . cord-264359-m9j3pcj1 149 1 thus thus RB cord-264359-m9j3pcj1 149 2 showing show VBG cord-264359-m9j3pcj1 149 3 the the DT cord-264359-m9j3pcj1 149 4 original original JJ cord-264359-m9j3pcj1 149 5 Cl Cl NNP cord-264359-m9j3pcj1 149 6 peak peak NN cord-264359-m9j3pcj1 149 7 to to TO cord-264359-m9j3pcj1 149 8 contain contain VB cord-264359-m9j3pcj1 149 9 a a DT cord-264359-m9j3pcj1 149 10 single single JJ cord-264359-m9j3pcj1 149 11 molecular molecular JJ cord-264359-m9j3pcj1 149 12 species specie NNS cord-264359-m9j3pcj1 149 13 . . . cord-264359-m9j3pcj1 150 1 This this DT cord-264359-m9j3pcj1 150 2 also also RB cord-264359-m9j3pcj1 150 3 held hold VBD cord-264359-m9j3pcj1 150 4 true true JJ cord-264359-m9j3pcj1 150 5 for for IN cord-264359-m9j3pcj1 150 6 peaks peak NNS cord-264359-m9j3pcj1 150 7 C5 C5 NNP cord-264359-m9j3pcj1 150 8 ( ( -LRB- cord-264359-m9j3pcj1 150 9 Figs Figs NNP cord-264359-m9j3pcj1 150 10 . . . cord-264359-m9j3pcj1 151 1 BD-8F BD-8F NNP cord-264359-m9j3pcj1 151 2 ) ) -RRB- cord-264359-m9j3pcj1 151 3 and and CC cord-264359-m9j3pcj1 151 4 C6 C6 NNP cord-264359-m9j3pcj1 151 5 ( ( -LRB- cord-264359-m9j3pcj1 151 6 Figs Figs NNP cord-264359-m9j3pcj1 151 7 . . . cord-264359-m9j3pcj1 151 8 8F-8 8f-8 CD cord-264359-m9j3pcj1 151 9 G g NN cord-264359-m9j3pcj1 151 10 ) ) -RRB- cord-264359-m9j3pcj1 151 11 ; ; : cord-264359-m9j3pcj1 152 1 ( ( -LRB- cord-264359-m9j3pcj1 152 2 iii iii NN cord-264359-m9j3pcj1 152 3 ) ) -RRB- cord-264359-m9j3pcj1 152 4 material material NN cord-264359-m9j3pcj1 152 5 collected collect VBN cord-264359-m9j3pcj1 152 6 between between IN cord-264359-m9j3pcj1 152 7 two two CD cord-264359-m9j3pcj1 152 8 of of IN cord-264359-m9j3pcj1 152 9 the the DT cord-264359-m9j3pcj1 152 10 original original JJ cord-264359-m9j3pcj1 152 11 peaks peak NNS cord-264359-m9j3pcj1 152 12 again again RB cord-264359-m9j3pcj1 152 13 yielded yield VBD cord-264359-m9j3pcj1 152 14 two two CD cord-264359-m9j3pcj1 152 15 peaks peak NNS cord-264359-m9j3pcj1 152 16 ( ( -LRB- cord-264359-m9j3pcj1 152 17 Figs fig NNS cord-264359-m9j3pcj1 152 18 . . . cord-264359-m9j3pcj1 153 1 BB BB NNP cord-264359-m9j3pcj1 153 2 , , , cord-264359-m9j3pcj1 153 3 8D 8d NN cord-264359-m9j3pcj1 153 4 , , , cord-264359-m9j3pcj1 153 5 and and CC cord-264359-m9j3pcj1 153 6 8F 8f NN cord-264359-m9j3pcj1 153 7 ) ) -RRB- cord-264359-m9j3pcj1 154 1 ; ; : cord-264359-m9j3pcj1 154 2 ( ( -LRB- cord-264359-m9j3pcj1 154 3 iv iv LS cord-264359-m9j3pcj1 154 4 ) ) -RRB- cord-264359-m9j3pcj1 154 5 C3 C3 NNP cord-264359-m9j3pcj1 154 6 and and CC cord-264359-m9j3pcj1 154 7 C4 C4 NNP cord-264359-m9j3pcj1 154 8 were be VBD cord-264359-m9j3pcj1 154 9 not not RB cord-264359-m9j3pcj1 154 10 resolved resolve VBN cord-264359-m9j3pcj1 154 11 , , , cord-264359-m9j3pcj1 154 12 but but CC cord-264359-m9j3pcj1 154 13 the the DT cord-264359-m9j3pcj1 154 14 presence presence NN cord-264359-m9j3pcj1 154 15 of of IN cord-264359-m9j3pcj1 154 16 two two CD cord-264359-m9j3pcj1 154 17 kinds kind NNS cord-264359-m9j3pcj1 154 18 of of IN cord-264359-m9j3pcj1 154 19 molecules molecule NNS cord-264359-m9j3pcj1 154 20 was be VBD cord-264359-m9j3pcj1 154 21 demonstrated demonstrate VBN cord-264359-m9j3pcj1 154 22 by by IN cord-264359-m9j3pcj1 154 23 the the DT cord-264359-m9j3pcj1 154 24 progressive progressive JJ cord-264359-m9j3pcj1 154 25 shift shift NN cord-264359-m9j3pcj1 154 26 to to IN cord-264359-m9j3pcj1 154 27 the the DT cord-264359-m9j3pcj1 154 28 right right NN cord-264359-m9j3pcj1 154 29 upon upon IN cord-264359-m9j3pcj1 154 30 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 154 31 of of IN cord-264359-m9j3pcj1 154 32 successive successive JJ cord-264359-m9j3pcj1 154 33 fractions fraction NNS cord-264359-m9j3pcj1 154 34 ( ( -LRB- cord-264359-m9j3pcj1 154 35 Figs fig NNS cord-264359-m9j3pcj1 154 36 . . . cord-264359-m9j3pcj1 154 37 BC-8D BC-8D NNP cord-264359-m9j3pcj1 154 38 ) ) -RRB- cord-264359-m9j3pcj1 154 39 . . . cord-264359-m9j3pcj1 155 1 This this DT cord-264359-m9j3pcj1 155 2 single single JJ cord-264359-m9j3pcj1 155 3 - - HYPH cord-264359-m9j3pcj1 155 4 slice slice NN cord-264359-m9j3pcj1 155 5 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 155 6 experiment experiment NN cord-264359-m9j3pcj1 155 7 allows allow VBZ cord-264359-m9j3pcj1 155 8 two two CD cord-264359-m9j3pcj1 155 9 conclusions conclusion NNS cord-264359-m9j3pcj1 155 10 : : : cord-264359-m9j3pcj1 155 11 ( ( -LRB- cord-264359-m9j3pcj1 155 12 i i NN cord-264359-m9j3pcj1 155 13 ) ) -RRB- cord-264359-m9j3pcj1 156 1 No no DT cord-264359-m9j3pcj1 156 2 interconversion interconversion NN cord-264359-m9j3pcj1 156 3 among among IN cord-264359-m9j3pcj1 156 4 doublet doublet NN cord-264359-m9j3pcj1 156 5 partners partner NNS cord-264359-m9j3pcj1 156 6 could could MD cord-264359-m9j3pcj1 156 7 be be VB cord-264359-m9j3pcj1 156 8 demonstrated demonstrate VBN cord-264359-m9j3pcj1 156 9 , , , cord-264359-m9j3pcj1 156 10 and and CC cord-264359-m9j3pcj1 156 11 ( ( -LRB- cord-264359-m9j3pcj1 156 12 ii ii NN cord-264359-m9j3pcj1 156 13 ) ) -RRB- cord-264359-m9j3pcj1 156 14 at at IN cord-264359-m9j3pcj1 156 15 least least JJS cord-264359-m9j3pcj1 156 16 those those DT cord-264359-m9j3pcj1 156 17 components component NNS cord-264359-m9j3pcj1 156 18 which which WDT cord-264359-m9j3pcj1 156 19 could could MD cord-264359-m9j3pcj1 156 20 be be VB cord-264359-m9j3pcj1 156 21 clearly clearly RB cord-264359-m9j3pcj1 156 22 resolved resolve VBN cord-264359-m9j3pcj1 156 23 in in IN cord-264359-m9j3pcj1 156 24 the the DT cord-264359-m9j3pcj1 156 25 primary primary JJ cord-264359-m9j3pcj1 156 26 radioactivity radioactivity NN cord-264359-m9j3pcj1 156 27 profile profile NN cord-264359-m9j3pcj1 156 28 ( ( -LRB- cord-264359-m9j3pcj1 156 29 i.e. i.e. FW cord-264359-m9j3pcj1 156 30 , , , cord-264359-m9j3pcj1 156 31 Cl Cl NNP cord-264359-m9j3pcj1 156 32 , , , cord-264359-m9j3pcj1 156 33 C5 C5 NNP cord-264359-m9j3pcj1 156 34 , , , cord-264359-m9j3pcj1 156 35 and and CC cord-264359-m9j3pcj1 156 36 CS CS NNP cord-264359-m9j3pcj1 156 37 ) ) -RRB- cord-264359-m9j3pcj1 156 38 ran run VBD cord-264359-m9j3pcj1 156 39 true true JJ cord-264359-m9j3pcj1 156 40 upon upon IN cord-264359-m9j3pcj1 156 41 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 156 42 . . . cord-264359-m9j3pcj1 157 1 DISCUSSION discussion NN cord-264359-m9j3pcj1 158 1 The the DT cord-264359-m9j3pcj1 158 2 improved improved JJ cord-264359-m9j3pcj1 158 3 resolution resolution NN cord-264359-m9j3pcj1 158 4 of of IN cord-264359-m9j3pcj1 158 5 poliovirus poliovirus NN cord-264359-m9j3pcj1 158 6 capsid capsid NN cord-264359-m9j3pcj1 158 7 polypeptides polypeptide NNS cord-264359-m9j3pcj1 158 8 by by IN cord-264359-m9j3pcj1 158 9 pH pH NNP cord-264359-m9j3pcj1 158 10 gradient gradient NNP cord-264359-m9j3pcj1 158 11 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 158 12 reported report VBN cord-264359-m9j3pcj1 158 13 by by IN cord-264359-m9j3pcj1 158 14 Vrijsen Vrijsen NNP cord-264359-m9j3pcj1 158 15 and and CC cord-264359-m9j3pcj1 158 16 Boeye Boeye NNP cord-264359-m9j3pcj1 158 17 ( ( -LRB- cord-264359-m9j3pcj1 158 18 1978 1978 CD cord-264359-m9j3pcj1 158 19 ) ) -RRB- cord-264359-m9j3pcj1 158 20 was be VBD cord-264359-m9j3pcj1 158 21 confirmed confirm VBN cord-264359-m9j3pcj1 158 22 and and CC cord-264359-m9j3pcj1 158 23 shown show VBN cord-264359-m9j3pcj1 158 24 to to TO cord-264359-m9j3pcj1 158 25 be be VB cord-264359-m9j3pcj1 158 26 independent independent JJ cord-264359-m9j3pcj1 158 27 of of IN cord-264359-m9j3pcj1 158 28 the the DT cord-264359-m9j3pcj1 158 29 serotype serotype NN cord-264359-m9j3pcj1 158 30 and and CC cord-264359-m9j3pcj1 158 31 of of IN cord-264359-m9j3pcj1 158 32 the the DT cord-264359-m9j3pcj1 158 33 methods method NNS cord-264359-m9j3pcj1 158 34 used use VBN cord-264359-m9j3pcj1 158 35 for for IN cord-264359-m9j3pcj1 158 36 virus virus NN cord-264359-m9j3pcj1 158 37 purification purification NN cord-264359-m9j3pcj1 158 38 and and CC cord-264359-m9j3pcj1 158 39 protein protein NN cord-264359-m9j3pcj1 158 40 extraction extraction NN cord-264359-m9j3pcj1 158 41 . . . cord-264359-m9j3pcj1 159 1 It -PRON- PRP cord-264359-m9j3pcj1 159 2 is be VBZ cord-264359-m9j3pcj1 159 3 therefore therefore RB cord-264359-m9j3pcj1 159 4 unlikely unlikely JJ cord-264359-m9j3pcj1 159 5 that that IN cord-264359-m9j3pcj1 159 6 the the DT cord-264359-m9j3pcj1 159 7 multiple multiple JJ cord-264359-m9j3pcj1 159 8 components component NNS cord-264359-m9j3pcj1 159 9 observed observe VBN cord-264359-m9j3pcj1 159 10 were be VBD cord-264359-m9j3pcj1 159 11 preparative preparative JJ cord-264359-m9j3pcj1 159 12 artifacts artifact NNS cord-264359-m9j3pcj1 159 13 , , , cord-264359-m9j3pcj1 159 14 as as IN cord-264359-m9j3pcj1 159 15 have have VBP cord-264359-m9j3pcj1 159 16 been be VBN cord-264359-m9j3pcj1 159 17 observed observe VBN cord-264359-m9j3pcj1 159 18 with with IN cord-264359-m9j3pcj1 159 19 proteins protein NNS cord-264359-m9j3pcj1 159 20 of of IN cord-264359-m9j3pcj1 159 21 coronaviruses coronaviruse NNS cord-264359-m9j3pcj1 159 22 ( ( -LRB- cord-264359-m9j3pcj1 159 23 Sturman Sturman NNP cord-264359-m9j3pcj1 159 24 , , , cord-264359-m9j3pcj1 159 25 1977 1977 CD cord-264359-m9j3pcj1 159 26 ) ) -RRB- cord-264359-m9j3pcj1 159 27 . . . cord-264359-m9j3pcj1 160 1 The the DT cord-264359-m9j3pcj1 160 2 possibility possibility NN cord-264359-m9j3pcj1 160 3 that that IN cord-264359-m9j3pcj1 160 4 they -PRON- PRP cord-264359-m9j3pcj1 160 5 were be VBD cord-264359-m9j3pcj1 160 6 formed form VBN cord-264359-m9j3pcj1 160 7 by by IN cord-264359-m9j3pcj1 160 8 partial partial JJ cord-264359-m9j3pcj1 160 9 degradation degradation NN cord-264359-m9j3pcj1 160 10 of of IN cord-264359-m9j3pcj1 160 11 the the DT cord-264359-m9j3pcj1 160 12 protein protein NN cord-264359-m9j3pcj1 160 13 during during IN cord-264359-m9j3pcj1 160 14 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 160 15 was be VBD cord-264359-m9j3pcj1 160 16 also also RB cord-264359-m9j3pcj1 160 17 examined examine VBN cord-264359-m9j3pcj1 160 18 and and CC cord-264359-m9j3pcj1 160 19 rejected reject VBN cord-264359-m9j3pcj1 160 20 . . . cord-264359-m9j3pcj1 161 1 Whereas whereas IN cord-264359-m9j3pcj1 161 2 normal normal JJ cord-264359-m9j3pcj1 161 3 SDS sds NN cord-264359-m9j3pcj1 161 4 - - HYPH cord-264359-m9j3pcj1 161 5 PAGE page NN cord-264359-m9j3pcj1 161 6 or or CC cord-264359-m9j3pcj1 161 7 discontinuous discontinuous JJ cord-264359-m9j3pcj1 161 8 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 161 9 according accord VBG cord-264359-m9j3pcj1 161 10 to to IN cord-264359-m9j3pcj1 161 11 Laemmli Laemmli NNP cord-264359-m9j3pcj1 161 12 ( ( -LRB- cord-264359-m9j3pcj1 161 13 1970 1970 CD cord-264359-m9j3pcj1 161 14 ) ) -RRB- cord-264359-m9j3pcj1 161 15 resolved resolve VBN cord-264359-m9j3pcj1 161 16 only only RB cord-264359-m9j3pcj1 161 17 VPl vpl NN cord-264359-m9j3pcj1 161 18 , , , cord-264359-m9j3pcj1 161 19 VP2 vp2 NN cord-264359-m9j3pcj1 161 20 , , , cord-264359-m9j3pcj1 161 21 and and CC cord-264359-m9j3pcj1 161 22 VP3 VP3 NNP cord-264359-m9j3pcj1 161 23 , , , cord-264359-m9j3pcj1 161 24 pH pH NNP cord-264359-m9j3pcj1 161 25 gradient gradient NN cord-264359-m9j3pcj1 161 26 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 161 27 usually usually RB cord-264359-m9j3pcj1 161 28 resolves resolve VBZ cord-264359-m9j3pcj1 161 29 six six CD cord-264359-m9j3pcj1 161 30 components component NNS cord-264359-m9j3pcj1 161 31 ( ( -LRB- cord-264359-m9j3pcj1 161 32 Cl Cl NNP cord-264359-m9j3pcj1 161 33 - - HYPH cord-264359-m9j3pcj1 161 34 CS CS NNP cord-264359-m9j3pcj1 161 35 ) ) -RRB- cord-264359-m9j3pcj1 161 36 . . . cord-264359-m9j3pcj1 162 1 The the DT cord-264359-m9j3pcj1 162 2 pattern pattern NN cord-264359-m9j3pcj1 162 3 was be VBD cord-264359-m9j3pcj1 162 4 not not RB cord-264359-m9j3pcj1 162 5 entirely entirely RB cord-264359-m9j3pcj1 162 6 constant constant JJ cord-264359-m9j3pcj1 162 7 , , , cord-264359-m9j3pcj1 162 8 however however RB cord-264359-m9j3pcj1 162 9 , , , cord-264359-m9j3pcj1 162 10 as as IN cord-264359-m9j3pcj1 162 11 neighboring neighboring NN cord-264359-m9j3pcj1 162 12 components component NNS cord-264359-m9j3pcj1 162 13 sometimes sometimes RB cord-264359-m9j3pcj1 162 14 failed fail VBD cord-264359-m9j3pcj1 162 15 to to TO cord-264359-m9j3pcj1 162 16 separate separate VB cord-264359-m9j3pcj1 162 17 ( ( -LRB- cord-264359-m9j3pcj1 162 18 e.g. e.g. RB cord-264359-m9j3pcj1 162 19 , , , cord-264359-m9j3pcj1 162 20 C3-C4 C3-C4 NNP cord-264359-m9j3pcj1 162 21 in in IN cord-264359-m9j3pcj1 162 22 Fig Fig NNP cord-264359-m9j3pcj1 162 23 . . . cord-264359-m9j3pcj1 163 1 80 80 CD cord-264359-m9j3pcj1 164 1 ; ; : cord-264359-m9j3pcj1 164 2 C5-C6 C5-C6 NNP cord-264359-m9j3pcj1 164 3 in in IN cord-264359-m9j3pcj1 164 4 Fig Fig NNP cord-264359-m9j3pcj1 164 5 . . NNP cord-264359-m9j3pcj1 164 6 4 4 LS cord-264359-m9j3pcj1 164 7 ) ) -RRB- cord-264359-m9j3pcj1 164 8 ; ; : cord-264359-m9j3pcj1 164 9 on on IN cord-264359-m9j3pcj1 164 10 the the DT cord-264359-m9j3pcj1 164 11 other other JJ cord-264359-m9j3pcj1 164 12 hand hand NN cord-264359-m9j3pcj1 164 13 , , , cord-264359-m9j3pcj1 164 14 the the DT cord-264359-m9j3pcj1 164 15 results result NNS cord-264359-m9j3pcj1 164 16 occasionally occasionally RB cord-264359-m9j3pcj1 164 17 suggested suggest VBD cord-264359-m9j3pcj1 164 18 further further JJ cord-264359-m9j3pcj1 164 19 resolution resolution NN cord-264359-m9j3pcj1 164 20 of of IN cord-264359-m9j3pcj1 164 21 a a DT cord-264359-m9j3pcj1 164 22 single single JJ cord-264359-m9j3pcj1 164 23 component component NN cord-264359-m9j3pcj1 164 24 ( ( -LRB- cord-264359-m9j3pcj1 164 25 e.g. e.g. RB cord-264359-m9j3pcj1 164 26 , , , cord-264359-m9j3pcj1 164 27 C3 C3 NNP cord-264359-m9j3pcj1 164 28 in in IN cord-264359-m9j3pcj1 164 29 Figs Figs NNPS cord-264359-m9j3pcj1 164 30 . . . cord-264359-m9j3pcj1 165 1 4 4 CD cord-264359-m9j3pcj1 165 2 and and CC cord-264359-m9j3pcj1 165 3 5D-5F 5d-5f CD cord-264359-m9j3pcj1 165 4 ) ) -RRB- cord-264359-m9j3pcj1 166 1 . . . cord-264359-m9j3pcj1 167 1 Due due IN cord-264359-m9j3pcj1 167 2 to to IN cord-264359-m9j3pcj1 167 3 these these DT cord-264359-m9j3pcj1 167 4 and and CC cord-264359-m9j3pcj1 167 5 other other JJ cord-264359-m9j3pcj1 167 6 technical technical JJ cord-264359-m9j3pcj1 167 7 difficulties difficulty NNS cord-264359-m9j3pcj1 167 8 , , , cord-264359-m9j3pcj1 167 9 no no DT cord-264359-m9j3pcj1 167 10 single single JJ cord-264359-m9j3pcj1 167 11 experiment experiment NN cord-264359-m9j3pcj1 167 12 yielded yield VBD cord-264359-m9j3pcj1 167 13 all all DT cord-264359-m9j3pcj1 167 14 of of IN cord-264359-m9j3pcj1 167 15 the the DT cord-264359-m9j3pcj1 167 16 information information NN cord-264359-m9j3pcj1 167 17 being be VBG cord-264359-m9j3pcj1 167 18 sought seek VBN cord-264359-m9j3pcj1 167 19 , , , cord-264359-m9j3pcj1 167 20 and and CC cord-264359-m9j3pcj1 167 21 a a DT cord-264359-m9j3pcj1 167 22 clear clear JJ cord-264359-m9j3pcj1 167 23 picture picture NN cord-264359-m9j3pcj1 167 24 emerged emerge VBD cord-264359-m9j3pcj1 167 25 only only RB cord-264359-m9j3pcj1 167 26 by by IN cord-264359-m9j3pcj1 167 27 juxtaposition juxtaposition NN cord-264359-m9j3pcj1 167 28 of of IN cord-264359-m9j3pcj1 167 29 the the DT cord-264359-m9j3pcj1 167 30 results result NNS cord-264359-m9j3pcj1 167 31 . . . cord-264359-m9j3pcj1 168 1 According accord VBG cord-264359-m9j3pcj1 168 2 to to IN cord-264359-m9j3pcj1 168 3 this this DT cord-264359-m9j3pcj1 168 4 picture picture NN cord-264359-m9j3pcj1 168 5 , , , cord-264359-m9j3pcj1 168 6 Cl Cl NNP cord-264359-m9j3pcj1 168 7 and and CC cord-264359-m9j3pcj1 168 8 C2 C2 NNP cord-264359-m9j3pcj1 168 9 formed form VBD cord-264359-m9j3pcj1 168 10 a a DT cord-264359-m9j3pcj1 168 11 doublet doublet NN cord-264359-m9j3pcj1 168 12 corresponding correspond VBG cord-264359-m9j3pcj1 168 13 to to IN cord-264359-m9j3pcj1 168 14 VPl VPl NNP cord-264359-m9j3pcj1 168 15 , , , cord-264359-m9j3pcj1 168 16 C3 C3 NNP cord-264359-m9j3pcj1 168 17 and and CC cord-264359-m9j3pcj1 168 18 C4 C4 NNP cord-264359-m9j3pcj1 168 19 a a DT cord-264359-m9j3pcj1 168 20 second second JJ cord-264359-m9j3pcj1 168 21 doublet doublet NN cord-264359-m9j3pcj1 168 22 corresponding correspond VBG cord-264359-m9j3pcj1 168 23 to to IN cord-264359-m9j3pcj1 168 24 VP2 vp2 NN cord-264359-m9j3pcj1 168 25 , , , cord-264359-m9j3pcj1 168 26 and and CC cord-264359-m9j3pcj1 168 27 C5 C5 NNP cord-264359-m9j3pcj1 168 28 and and CC cord-264359-m9j3pcj1 168 29 C6 C6 NNP cord-264359-m9j3pcj1 168 30 a a DT cord-264359-m9j3pcj1 168 31 third third JJ cord-264359-m9j3pcj1 168 32 doublet doublet NN cord-264359-m9j3pcj1 168 33 corresponding correspond VBG cord-264359-m9j3pcj1 168 34 to to IN cord-264359-m9j3pcj1 168 35 VP3 VP3 NNP cord-264359-m9j3pcj1 168 36 . . . cord-264359-m9j3pcj1 169 1 With with IN cord-264359-m9j3pcj1 169 2 regard regard NN cord-264359-m9j3pcj1 169 3 to to IN cord-264359-m9j3pcj1 169 4 the the DT cord-264359-m9j3pcj1 169 5 Cl Cl NNP cord-264359-m9j3pcj1 169 6 - - HYPH cord-264359-m9j3pcj1 169 7 C2 C2 NNP cord-264359-m9j3pcj1 169 8 doublet doublet NN cord-264359-m9j3pcj1 169 9 , , , cord-264359-m9j3pcj1 169 10 for for IN cord-264359-m9j3pcj1 169 11 example example NN cord-264359-m9j3pcj1 169 12 , , , cord-264359-m9j3pcj1 169 13 this this DT cord-264359-m9j3pcj1 169 14 correspondence correspondence NN cord-264359-m9j3pcj1 169 15 means mean VBZ cord-264359-m9j3pcj1 169 16 ( ( -LRB- cord-264359-m9j3pcj1 169 17 i i NN cord-264359-m9j3pcj1 169 18 ) ) -RRB- cord-264359-m9j3pcj1 169 19 that that IN cord-264359-m9j3pcj1 169 20 the the DT cord-264359-m9j3pcj1 169 21 molecules molecule NNS cord-264359-m9j3pcj1 169 22 which which WDT cord-264359-m9j3pcj1 169 23 migrate migrate VBP cord-264359-m9j3pcj1 169 24 as as IN cord-264359-m9j3pcj1 169 25 either either DT cord-264359-m9j3pcj1 169 26 Cl Cl NNP cord-264359-m9j3pcj1 169 27 or or CC cord-264359-m9j3pcj1 169 28 C2 C2 NNP cord-264359-m9j3pcj1 169 29 in in IN cord-264359-m9j3pcj1 169 30 pH pH NNP cord-264359-m9j3pcj1 169 31 gradient gradient NN cord-264359-m9j3pcj1 169 32 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 169 33 will will MD cord-264359-m9j3pcj1 169 34 migrate migrate VB cord-264359-m9j3pcj1 169 35 as as IN cord-264359-m9j3pcj1 169 36 VP1 VP1 NNP cord-264359-m9j3pcj1 169 37 in in IN cord-264359-m9j3pcj1 169 38 normal normal JJ cord-264359-m9j3pcj1 169 39 SDS sds NN cord-264359-m9j3pcj1 169 40 - - HYPH cord-264359-m9j3pcj1 169 41 PAGE PAGE NNP cord-264359-m9j3pcj1 169 42 , , , cord-264359-m9j3pcj1 169 43 and and CC cord-264359-m9j3pcj1 169 44 ( ( -LRB- cord-264359-m9j3pcj1 169 45 ii ii NN cord-264359-m9j3pcj1 169 46 ) ) -RRB- cord-264359-m9j3pcj1 169 47 that that IN cord-264359-m9j3pcj1 169 48 Cl Cl NNP cord-264359-m9j3pcj1 169 49 and and CC cord-264359-m9j3pcj1 169 50 C2 C2 NNP cord-264359-m9j3pcj1 169 51 in in IN cord-264359-m9j3pcj1 169 52 emetine emetine NNP cord-264359-m9j3pcj1 169 53 - - HYPH cord-264359-m9j3pcj1 169 54 stopped stop VBN cord-264359-m9j3pcj1 169 55 pulses pulse NNS cord-264359-m9j3pcj1 169 56 are be VBP cord-264359-m9j3pcj1 169 57 labeled label VBN cord-264359-m9j3pcj1 169 58 equally equally RB cord-264359-m9j3pcj1 169 59 and and CC cord-264359-m9j3pcj1 169 60 according accord VBG cord-264359-m9j3pcj1 169 61 to to IN cord-264359-m9j3pcj1 169 62 the the DT cord-264359-m9j3pcj1 169 63 same same JJ cord-264359-m9j3pcj1 169 64 kinetics kinetics NN cord-264359-m9j3pcj1 169 65 as as IN cord-264359-m9j3pcj1 169 66 VPl VPl NNP cord-264359-m9j3pcj1 169 67 . . . cord-264359-m9j3pcj1 170 1 Thus thus RB cord-264359-m9j3pcj1 170 2 , , , cord-264359-m9j3pcj1 170 3 although although IN cord-264359-m9j3pcj1 170 4 we -PRON- PRP cord-264359-m9j3pcj1 170 5 demonstrated demonstrate VBD cord-264359-m9j3pcj1 170 6 a a DT cord-264359-m9j3pcj1 170 7 higher high JJR cord-264359-m9j3pcj1 170 8 number number NN cord-264359-m9j3pcj1 170 9 of of IN cord-264359-m9j3pcj1 170 10 electrophoretie electrophoretie NN cord-264359-m9j3pcj1 170 11 components component NNS cord-264359-m9j3pcj1 170 12 , , , cord-264359-m9j3pcj1 170 13 our -PRON- PRP$ cord-264359-m9j3pcj1 170 14 findings finding NNS cord-264359-m9j3pcj1 170 15 are be VBP cord-264359-m9j3pcj1 170 16 still still RB cord-264359-m9j3pcj1 170 17 basically basically RB cord-264359-m9j3pcj1 170 18 in in IN cord-264359-m9j3pcj1 170 19 agreement agreement NN cord-264359-m9j3pcj1 170 20 with with IN cord-264359-m9j3pcj1 170 21 the the DT cord-264359-m9j3pcj1 170 22 accepted accept VBN cord-264359-m9j3pcj1 170 23 VPl-2 vpl-2 NN cord-264359-m9j3pcj1 170 24 - - HYPH cord-264359-m9j3pcj1 170 25 3 3 CD cord-264359-m9j3pcj1 170 26 - - SYM cord-264359-m9j3pcj1 170 27 4 4 CD cord-264359-m9j3pcj1 170 28 scheme scheme NN cord-264359-m9j3pcj1 170 29 for for IN cord-264359-m9j3pcj1 170 30 the the DT cord-264359-m9j3pcj1 170 31 composition composition NN cord-264359-m9j3pcj1 170 32 of of IN cord-264359-m9j3pcj1 170 33 the the DT cord-264359-m9j3pcj1 170 34 poliovirus poliovirus NN cord-264359-m9j3pcj1 170 35 capsid capsid NN cord-264359-m9j3pcj1 170 36 . . . cord-264359-m9j3pcj1 171 1 Several several JJ cord-264359-m9j3pcj1 171 2 SDS SDS NNP cord-264359-m9j3pcj1 171 3 complexes complex NNS cord-264359-m9j3pcj1 171 4 of of IN cord-264359-m9j3pcj1 171 5 the the DT cord-264359-m9j3pcj1 171 6 same same JJ cord-264359-m9j3pcj1 171 7 pro pro JJ cord-264359-m9j3pcj1 171 8 - - JJ cord-264359-m9j3pcj1 171 9 VRIJSEN vrijsen JJ cord-264359-m9j3pcj1 171 10 , , , cord-264359-m9j3pcj1 171 11 WOUTERS WOUTERS NNP cord-264359-m9j3pcj1 171 12 , , , cord-264359-m9j3pcj1 171 13 AND and CC cord-264359-m9j3pcj1 171 14 BOEYE BOEYE NNP cord-264359-m9j3pcj1 171 15 tein tein NNP cord-264359-m9j3pcj1 171 16 may may MD cord-264359-m9j3pcj1 171 17 coexist coexist VB cord-264359-m9j3pcj1 171 18 , , , cord-264359-m9j3pcj1 171 19 at at IN cord-264359-m9j3pcj1 171 20 least least JJS cord-264359-m9j3pcj1 171 21 when when WRB cord-264359-m9j3pcj1 171 22 a a DT cord-264359-m9j3pcj1 171 23 limited limited JJ cord-264359-m9j3pcj1 171 24 amount amount NN cord-264359-m9j3pcj1 171 25 of of IN cord-264359-m9j3pcj1 171 26 SDS SDS NNP cord-264359-m9j3pcj1 171 27 is be VBZ cord-264359-m9j3pcj1 171 28 made make VBN cord-264359-m9j3pcj1 171 29 available available JJ cord-264359-m9j3pcj1 171 30 for for IN cord-264359-m9j3pcj1 171 31 binding bind VBG cord-264359-m9j3pcj1 171 32 ; ; : cord-264359-m9j3pcj1 171 33 however however RB cord-264359-m9j3pcj1 171 34 , , , cord-264359-m9j3pcj1 171 35 saturation saturation NN cord-264359-m9j3pcj1 171 36 is be VBZ cord-264359-m9j3pcj1 171 37 reached reach VBN cord-264359-m9j3pcj1 171 38 at at IN cord-264359-m9j3pcj1 171 39 a a DT cord-264359-m9j3pcj1 171 40 SDS SDS NNP cord-264359-m9j3pcj1 171 41 / / SYM cord-264359-m9j3pcj1 171 42 protein protein NN cord-264359-m9j3pcj1 171 43 weight weight NN cord-264359-m9j3pcj1 171 44 ratio ratio NN cord-264359-m9j3pcj1 171 45 of of IN cord-264359-m9j3pcj1 171 46 about about RB cord-264359-m9j3pcj1 171 47 4.5:1 4.5:1 CD cord-264359-m9j3pcj1 172 1 ( ( -LRB- cord-264359-m9j3pcj1 172 2 Stoklosa Stoklosa NNP cord-264359-m9j3pcj1 172 3 and and CC cord-264359-m9j3pcj1 172 4 Latz Latz NNP cord-264359-m9j3pcj1 172 5 , , , cord-264359-m9j3pcj1 172 6 1975 1975 CD cord-264359-m9j3pcj1 172 7 ) ) -RRB- cord-264359-m9j3pcj1 172 8 ? ? . cord-264359-m9j3pcj1 173 1 and and CC cord-264359-m9j3pcj1 173 2 this this DT cord-264359-m9j3pcj1 173 3 ratio ratio NN cord-264359-m9j3pcj1 173 4 was be VBD cord-264359-m9j3pcj1 173 5 largely largely RB cord-264359-m9j3pcj1 173 6 exceeded exceed VBN cord-264359-m9j3pcj1 173 7 in in IN cord-264359-m9j3pcj1 173 8 our -PRON- PRP$ cord-264359-m9j3pcj1 173 9 extraction extraction NN cord-264359-m9j3pcj1 173 10 procedure procedure NN cord-264359-m9j3pcj1 174 1 ( ( -LRB- cord-264359-m9j3pcj1 174 2 SDS SDS NNP cord-264359-m9j3pcj1 174 3 / / SYM cord-264359-m9j3pcj1 174 4 protein protein NN cord-264359-m9j3pcj1 174 5 ratio ratio NN cord-264359-m9j3pcj1 174 6 ca ca NNP cord-264359-m9j3pcj1 174 7 3O 3o CD cord-264359-m9j3pcj1 175 1 : : : cord-264359-m9j3pcj1 175 2 l l NNP cord-264359-m9j3pcj1 175 3 ) ) -RRB- cord-264359-m9j3pcj1 175 4 and and CC cord-264359-m9j3pcj1 175 5 during during IN cord-264359-m9j3pcj1 175 6 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 175 7 ( ( -LRB- cord-264359-m9j3pcj1 175 8 unlimited unlimited JJ cord-264359-m9j3pcj1 175 9 supply supply NN cord-264359-m9j3pcj1 175 10 of of IN cord-264359-m9j3pcj1 175 11 SDS SDS NNP cord-264359-m9j3pcj1 175 12 ) ) -RRB- cord-264359-m9j3pcj1 175 13 . . . cord-264359-m9j3pcj1 176 1 Even even RB cord-264359-m9j3pcj1 176 2 so so RB cord-264359-m9j3pcj1 176 3 , , , cord-264359-m9j3pcj1 176 4 the the DT cord-264359-m9j3pcj1 176 5 question question NN cord-264359-m9j3pcj1 176 6 may may MD cord-264359-m9j3pcj1 176 7 be be VB cord-264359-m9j3pcj1 176 8 asked ask VBN cord-264359-m9j3pcj1 176 9 whether whether IN cord-264359-m9j3pcj1 176 10 the the DT cord-264359-m9j3pcj1 176 11 two two CD cord-264359-m9j3pcj1 176 12 electrophoretic electrophoretic JJ cord-264359-m9j3pcj1 176 13 components component NNS cord-264359-m9j3pcj1 176 14 in in IN cord-264359-m9j3pcj1 176 15 each each DT cord-264359-m9j3pcj1 176 16 doublet doublet NN cord-264359-m9j3pcj1 176 17 could could MD cord-264359-m9j3pcj1 176 18 not not RB cord-264359-m9j3pcj1 176 19 be be VB cord-264359-m9j3pcj1 176 20 different different JJ cord-264359-m9j3pcj1 176 21 dodecyl dodecyl JJ cord-264359-m9j3pcj1 176 22 sulfate sulfate NN cord-264359-m9j3pcj1 176 23 complexes complex NNS cord-264359-m9j3pcj1 176 24 of of IN cord-264359-m9j3pcj1 176 25 the the DT cord-264359-m9j3pcj1 176 26 same same JJ cord-264359-m9j3pcj1 176 27 polypeptide polypeptide NN cord-264359-m9j3pcj1 176 28 . . . cord-264359-m9j3pcj1 177 1 Therefore therefore RB cord-264359-m9j3pcj1 177 2 , , , cord-264359-m9j3pcj1 177 3 we -PRON- PRP cord-264359-m9j3pcj1 177 4 looked look VBD cord-264359-m9j3pcj1 177 5 for for IN cord-264359-m9j3pcj1 177 6 possible possible JJ cord-264359-m9j3pcj1 177 7 interconversion interconversion NN cord-264359-m9j3pcj1 177 8 to to IN cord-264359-m9j3pcj1 177 9 the the DT cord-264359-m9j3pcj1 177 10 doublet doublet NN cord-264359-m9j3pcj1 177 11 partner partner NN cord-264359-m9j3pcj1 177 12 upon upon IN cord-264359-m9j3pcj1 177 13 isolation isolation NN cord-264359-m9j3pcj1 177 14 of of IN cord-264359-m9j3pcj1 177 15 single single JJ cord-264359-m9j3pcj1 177 16 components component NNS cord-264359-m9j3pcj1 177 17 and and CC cord-264359-m9j3pcj1 177 18 exact exact JJ cord-264359-m9j3pcj1 177 19 recreation recreation NN cord-264359-m9j3pcj1 177 20 of of IN cord-264359-m9j3pcj1 177 21 the the DT cord-264359-m9j3pcj1 177 22 conditions condition NNS cord-264359-m9j3pcj1 177 23 under under IN cord-264359-m9j3pcj1 177 24 which which WDT cord-264359-m9j3pcj1 177 25 the the DT cord-264359-m9j3pcj1 177 26 protein protein NN cord-264359-m9j3pcj1 177 27 - - HYPH cord-264359-m9j3pcj1 177 28 detergent detergent NN cord-264359-m9j3pcj1 177 29 complexes complex NNS cord-264359-m9j3pcj1 177 30 were be VBD cord-264359-m9j3pcj1 177 31 originally originally RB cord-264359-m9j3pcj1 177 32 formed form VBN cord-264359-m9j3pcj1 177 33 ( ( -LRB- cord-264359-m9j3pcj1 177 34 extraction extraction NN cord-264359-m9j3pcj1 177 35 of of IN cord-264359-m9j3pcj1 177 36 capsid capsid NN cord-264359-m9j3pcj1 177 37 protein protein NN cord-264359-m9j3pcj1 177 38 ) ) -RRB- cord-264359-m9j3pcj1 177 39 . . . cord-264359-m9j3pcj1 178 1 Since since IN cord-264359-m9j3pcj1 178 2 no no DT cord-264359-m9j3pcj1 178 3 interconversion interconversion NN cord-264359-m9j3pcj1 178 4 was be VBD cord-264359-m9j3pcj1 178 5 found find VBN cord-264359-m9j3pcj1 178 6 and and CC cord-264359-m9j3pcj1 178 7 each each DT cord-264359-m9j3pcj1 178 8 component component NN cord-264359-m9j3pcj1 178 9 ran run VBD cord-264359-m9j3pcj1 178 10 true true JJ cord-264359-m9j3pcj1 178 11 upon upon IN cord-264359-m9j3pcj1 178 12 reelectrophoresis reelectrophoresis NN cord-264359-m9j3pcj1 178 13 , , , cord-264359-m9j3pcj1 178 14 it -PRON- PRP cord-264359-m9j3pcj1 178 15 may may MD cord-264359-m9j3pcj1 178 16 be be VB cord-264359-m9j3pcj1 178 17 tentatively tentatively RB cord-264359-m9j3pcj1 178 18 concluded conclude VBN cord-264359-m9j3pcj1 178 19 that that IN cord-264359-m9j3pcj1 178 20 the the DT cord-264359-m9j3pcj1 178 21 two two CD cord-264359-m9j3pcj1 178 22 polypeptides polypeptide NNS cord-264359-m9j3pcj1 178 23 in in IN cord-264359-m9j3pcj1 178 24 each each DT cord-264359-m9j3pcj1 178 25 doublet doublet NN cord-264359-m9j3pcj1 178 26 were be VBD cord-264359-m9j3pcj1 178 27 not not RB cord-264359-m9j3pcj1 178 28 identical identical JJ cord-264359-m9j3pcj1 178 29 . . . cord-264359-m9j3pcj1 179 1 If if IN cord-264359-m9j3pcj1 179 2 so so RB cord-264359-m9j3pcj1 179 3 , , , cord-264359-m9j3pcj1 179 4 they -PRON- PRP cord-264359-m9j3pcj1 179 5 must must MD cord-264359-m9j3pcj1 179 6 differ differ VB cord-264359-m9j3pcj1 179 7 slightly slightly RB cord-264359-m9j3pcj1 179 8 in in IN cord-264359-m9j3pcj1 179 9 charge charge NN cord-264359-m9j3pcj1 179 10 , , , cord-264359-m9j3pcj1 179 11 molecular molecular JJ cord-264359-m9j3pcj1 179 12 weight weight NN cord-264359-m9j3pcj1 179 13 , , , cord-264359-m9j3pcj1 179 14 or or CC cord-264359-m9j3pcj1 179 15 both both DT cord-264359-m9j3pcj1 179 16 . . . cord-264359-m9j3pcj1 180 1 Such such JJ cord-264359-m9j3pcj1 180 2 differences difference NNS cord-264359-m9j3pcj1 180 3 may may MD cord-264359-m9j3pcj1 180 4 conceivably conceivably RB cord-264359-m9j3pcj1 180 5 originate originate VB cord-264359-m9j3pcj1 180 6 in in IN cord-264359-m9j3pcj1 180 7 the the DT cord-264359-m9j3pcj1 180 8 accumulation accumulation NN cord-264359-m9j3pcj1 180 9 of of IN cord-264359-m9j3pcj1 180 10 mutants mutant NNS cord-264359-m9j3pcj1 180 11 as as IN cord-264359-m9j3pcj1 180 12 a a DT cord-264359-m9j3pcj1 180 13 result result NN cord-264359-m9j3pcj1 180 14 of of IN cord-264359-m9j3pcj1 180 15 serial serial JJ cord-264359-m9j3pcj1 180 16 passages passage NNS cord-264359-m9j3pcj1 180 17 ( ( -LRB- cord-264359-m9j3pcj1 180 18 Phillips Phillips NNP cord-264359-m9j3pcj1 180 19 and and CC cord-264359-m9j3pcj1 180 20 Fennell Fennell NNP cord-264359-m9j3pcj1 180 21 , , , cord-264359-m9j3pcj1 180 22 1973 1973 CD cord-264359-m9j3pcj1 180 23 ) ) -RRB- cord-264359-m9j3pcj1 180 24 . . . cord-264359-m9j3pcj1 181 1 To to TO cord-264359-m9j3pcj1 181 2 prevent prevent VB cord-264359-m9j3pcj1 181 3 any any DT cord-264359-m9j3pcj1 181 4 such such JJ cord-264359-m9j3pcj1 181 5 accumulation accumulation NN cord-264359-m9j3pcj1 181 6 , , , cord-264359-m9j3pcj1 181 7 however however RB cord-264359-m9j3pcj1 181 8 , , , cord-264359-m9j3pcj1 181 9 each each DT cord-264359-m9j3pcj1 181 10 virus virus NN cord-264359-m9j3pcj1 181 11 population population NN cord-264359-m9j3pcj1 181 12 examined examine VBD cord-264359-m9j3pcj1 181 13 in in IN cord-264359-m9j3pcj1 181 14 this this DT cord-264359-m9j3pcj1 181 15 work work NN cord-264359-m9j3pcj1 181 16 was be VBD cord-264359-m9j3pcj1 181 17 grown grow VBN cord-264359-m9j3pcj1 181 18 from from IN cord-264359-m9j3pcj1 181 19 a a DT cord-264359-m9j3pcj1 181 20 single single JJ cord-264359-m9j3pcj1 181 21 plaque plaque NN cord-264359-m9j3pcj1 181 22 by by IN cord-264359-m9j3pcj1 181 23 only only RB cord-264359-m9j3pcj1 181 24 four four CD cord-264359-m9j3pcj1 181 25 passages passage NNS cord-264359-m9j3pcj1 181 26 , , , cord-264359-m9j3pcj1 181 27 and and CC cord-264359-m9j3pcj1 181 28 a a DT cord-264359-m9j3pcj1 181 29 mutational mutational JJ cord-264359-m9j3pcj1 181 30 origin origin NN cord-264359-m9j3pcj1 181 31 of of IN cord-264359-m9j3pcj1 181 32 the the DT cord-264359-m9j3pcj1 181 33 observed observed JJ cord-264359-m9j3pcj1 181 34 multiplicity multiplicity NN cord-264359-m9j3pcj1 181 35 can can MD cord-264359-m9j3pcj1 181 36 therefore therefore RB cord-264359-m9j3pcj1 181 37 be be VB cord-264359-m9j3pcj1 181 38 excluded exclude VBN cord-264359-m9j3pcj1 181 39 . . . cord-264359-m9j3pcj1 182 1 The the DT cord-264359-m9j3pcj1 182 2 observed observed JJ cord-264359-m9j3pcj1 182 3 microheterogeneity microheterogeneity NN cord-264359-m9j3pcj1 182 4 probably probably RB cord-264359-m9j3pcj1 182 5 originates originate VBZ cord-264359-m9j3pcj1 182 6 in in IN cord-264359-m9j3pcj1 182 7 the the DT cord-264359-m9j3pcj1 182 8 posttranslational posttranslational JJ cord-264359-m9j3pcj1 182 9 processing processing NN cord-264359-m9j3pcj1 182 10 of of IN cord-264359-m9j3pcj1 182 11 the the DT cord-264359-m9j3pcj1 182 12 proteins protein NNS cord-264359-m9j3pcj1 182 13 . . . cord-264359-m9j3pcj1 183 1 Since since IN cord-264359-m9j3pcj1 183 2 the the DT cord-264359-m9j3pcj1 183 3 capsid capsid NN cord-264359-m9j3pcj1 183 4 proteins protein NNS cord-264359-m9j3pcj1 183 5 are be VBP cord-264359-m9j3pcj1 183 6 carved carve VBN cord-264359-m9j3pcj1 183 7 out out IN cord-264359-m9j3pcj1 183 8 of of IN cord-264359-m9j3pcj1 183 9 a a DT cord-264359-m9j3pcj1 183 10 common common JJ cord-264359-m9j3pcj1 183 11 precursor precursor NN cord-264359-m9j3pcj1 183 12 by by IN cord-264359-m9j3pcj1 183 13 successive successive JJ cord-264359-m9j3pcj1 183 14 cleavages cleavage NNS cord-264359-m9j3pcj1 183 15 ( ( -LRB- cord-264359-m9j3pcj1 183 16 Summers Summers NNPS cord-264359-m9j3pcj1 183 17 and and CC cord-264359-m9j3pcj1 183 18 Maizel Maizel NNP cord-264359-m9j3pcj1 183 19 , , , cord-264359-m9j3pcj1 183 20 1968 1968 CD cord-264359-m9j3pcj1 183 21 ; ; : cord-264359-m9j3pcj1 183 22 Jacobson Jacobson NNP cord-264359-m9j3pcj1 183 23 and and CC cord-264359-m9j3pcj1 183 24 Baltimore Baltimore NNP cord-264359-m9j3pcj1 183 25 , , , cord-264359-m9j3pcj1 183 26 1968 1968 CD cord-264359-m9j3pcj1 183 27 ) ) -RRB- cord-264359-m9j3pcj1 183 28 , , , cord-264359-m9j3pcj1 183 29 microheterogeneity microheterogeneity NN cord-264359-m9j3pcj1 183 30 may may MD cord-264359-m9j3pcj1 183 31 result result VB cord-264359-m9j3pcj1 183 32 from from IN cord-264359-m9j3pcj1 183 33 multiplicity multiplicity NN cord-264359-m9j3pcj1 183 34 of of IN cord-264359-m9j3pcj1 183 35 the the DT cord-264359-m9j3pcj1 183 36 cleavage cleavage NN cord-264359-m9j3pcj1 183 37 sites site NNS cord-264359-m9j3pcj1 183 38 . . . cord-264359-m9j3pcj1 184 1 Ambiguity ambiguity NN cord-264359-m9j3pcj1 184 2 of of IN cord-264359-m9j3pcj1 184 3 cleavage cleavage NN cord-264359-m9j3pcj1 184 4 was be VBD cord-264359-m9j3pcj1 184 5 already already RB cord-264359-m9j3pcj1 184 6 invoked invoke VBN cord-264359-m9j3pcj1 184 7 to to TO cord-264359-m9j3pcj1 184 8 explain explain VB cord-264359-m9j3pcj1 184 9 differences difference NNS cord-264359-m9j3pcj1 184 10 in in IN cord-264359-m9j3pcj1 184 11 the the DT cord-264359-m9j3pcj1 184 12 migration migration NN cord-264359-m9j3pcj1 184 13 velocity velocity NN cord-264359-m9j3pcj1 184 14 of of IN cord-264359-m9j3pcj1 184 15 homologous homologous JJ cord-264359-m9j3pcj1 184 16 polypeptides polypeptide NNS cord-264359-m9j3pcj1 184 17 of of IN cord-264359-m9j3pcj1 184 18 different different JJ cord-264359-m9j3pcj1 184 19 poliovirus poliovirus NN cord-264359-m9j3pcj1 184 20 strains strain NNS cord-264359-m9j3pcj1 184 21 ( ( -LRB- cord-264359-m9j3pcj1 184 22 Cooper Cooper NNP cord-264359-m9j3pcj1 184 23 et et NNP cord-264359-m9j3pcj1 184 24 al al NNP cord-264359-m9j3pcj1 184 25 . . NNP cord-264359-m9j3pcj1 184 26 , , , cord-264359-m9j3pcj1 184 27 1970 1970 CD cord-264359-m9j3pcj1 184 28 ) ) -RRB- cord-264359-m9j3pcj1 184 29 or or CC cord-264359-m9j3pcj1 184 30 the the DT cord-264359-m9j3pcj1 184 31 appearance appearance NN cord-264359-m9j3pcj1 184 32 of of IN cord-264359-m9j3pcj1 184 33 doublets doublet NNS cord-264359-m9j3pcj1 184 34 ( ( -LRB- cord-264359-m9j3pcj1 184 35 Beckman Beckman NNP cord-264359-m9j3pcj1 184 36 et et NNP cord-264359-m9j3pcj1 184 37 al al NNP cord-264359-m9j3pcj1 184 38 . . NNP cord-264359-m9j3pcj1 184 39 , , , cord-264359-m9j3pcj1 184 40 1976 1976 CD cord-264359-m9j3pcj1 184 41 ) ) -RRB- cord-264359-m9j3pcj1 184 42 . . . cord-264359-m9j3pcj1 185 1 However however RB cord-264359-m9j3pcj1 185 2 , , , cord-264359-m9j3pcj1 185 3 no no DT cord-264359-m9j3pcj1 185 4 direct direct JJ cord-264359-m9j3pcj1 185 5 proof proof NN cord-264359-m9j3pcj1 185 6 of of IN cord-264359-m9j3pcj1 185 7 ambiguity ambiguity NN cord-264359-m9j3pcj1 185 8 was be VBD cord-264359-m9j3pcj1 185 9 offered offer VBN cord-264359-m9j3pcj1 185 10 and and CC cord-264359-m9j3pcj1 185 11 the the DT cord-264359-m9j3pcj1 185 12 case case NN cord-264359-m9j3pcj1 185 13 rests rest VBZ cord-264359-m9j3pcj1 185 14 on on IN cord-264359-m9j3pcj1 185 15 the the DT cord-264359-m9j3pcj1 185 16 unproven unproven JJ cord-264359-m9j3pcj1 185 17 assumption assumption NN cord-264359-m9j3pcj1 185 18 that that IN cord-264359-m9j3pcj1 185 19 differences difference NNS cord-264359-m9j3pcj1 185 20 in in IN cord-264359-m9j3pcj1 185 21 migration migration NN cord-264359-m9j3pcj1 185 22 velocity velocity NN cord-264359-m9j3pcj1 185 23 in in IN cord-264359-m9j3pcj1 185 24 SDS SDS NNP cord-264359-m9j3pcj1 185 25 - - HYPH cord-264359-m9j3pcj1 185 26 PAGE PAGE NNP cord-264359-m9j3pcj1 185 27 necessarily necessarily RB cord-264359-m9j3pcj1 185 28 reflect reflect VBP cord-264359-m9j3pcj1 185 29 differences difference NNS cord-264359-m9j3pcj1 185 30 in in IN cord-264359-m9j3pcj1 185 31 molecular molecular JJ cord-264359-m9j3pcj1 185 32 weight weight NN cord-264359-m9j3pcj1 185 33 . . . cord-264359-m9j3pcj1 186 1 Ambiguity ambiguity NN cord-264359-m9j3pcj1 186 2 of of IN cord-264359-m9j3pcj1 186 3 cleavage cleavage NN cord-264359-m9j3pcj1 186 4 would would MD cord-264359-m9j3pcj1 186 5 be be VB cord-264359-m9j3pcj1 186 6 expected expect VBN cord-264359-m9j3pcj1 186 7 to to TO cord-264359-m9j3pcj1 186 8 reveal reveal VB cord-264359-m9j3pcj1 186 9 itself -PRON- PRP cord-264359-m9j3pcj1 186 10 in in IN cord-264359-m9j3pcj1 186 11 multiple multiple JJ cord-264359-m9j3pcj1 186 12 N n CD cord-264359-m9j3pcj1 186 13 - - HYPH cord-264359-m9j3pcj1 186 14 terminal terminal NN cord-264359-m9j3pcj1 186 15 or or CC cord-264359-m9j3pcj1 186 16 Cterminal Cterminal NNP cord-264359-m9j3pcj1 186 17 sequences sequence NNS cord-264359-m9j3pcj1 186 18 . . . cord-264359-m9j3pcj1 187 1 The the DT cord-264359-m9j3pcj1 187 2 terminal terminal JJ cord-264359-m9j3pcj1 187 3 sequences sequence NNS cord-264359-m9j3pcj1 187 4 of of IN cord-264359-m9j3pcj1 187 5 the the DT cord-264359-m9j3pcj1 187 6 major major JJ cord-264359-m9j3pcj1 187 7 capsid capsid NN cord-264359-m9j3pcj1 187 8 polypeptides polypeptide NNS cord-264359-m9j3pcj1 187 9 of of IN cord-264359-m9j3pcj1 187 10 Mengo Mengo NNP cord-264359-m9j3pcj1 187 11 virus virus NN cord-264359-m9j3pcj1 187 12 were be VBD cord-264359-m9j3pcj1 187 13 determined determined JJ cord-264359-m9j3pcj1 187 14 and and CC cord-264359-m9j3pcj1 187 15 no no DT cord-264359-m9j3pcj1 187 16 mention mention NN cord-264359-m9j3pcj1 187 17 was be VBD cord-264359-m9j3pcj1 187 18 made make VBN cord-264359-m9j3pcj1 187 19 of of IN cord-264359-m9j3pcj1 187 20 ambiguities ambiguity NNS cord-264359-m9j3pcj1 187 21 ( ( -LRB- cord-264359-m9j3pcj1 187 22 Ziola Ziola NNP cord-264359-m9j3pcj1 187 23 and and CC cord-264359-m9j3pcj1 187 24 Scraba Scraba NNP cord-264359-m9j3pcj1 187 25 , , , cord-264359-m9j3pcj1 187 26 1976 1976 CD cord-264359-m9j3pcj1 187 27 ) ) -RRB- cord-264359-m9j3pcj1 187 28 . . . cord-264359-m9j3pcj1 188 1 This this DT cord-264359-m9j3pcj1 188 2 is be VBZ cord-264359-m9j3pcj1 188 3 also also RB cord-264359-m9j3pcj1 188 4 true true JJ cord-264359-m9j3pcj1 188 5 for for IN cord-264359-m9j3pcj1 188 6 footand footand NN cord-264359-m9j3pcj1 188 7 - - HYPH cord-264359-m9j3pcj1 188 8 mouth mouth NN cord-264359-m9j3pcj1 188 9 disease disease NN cord-264359-m9j3pcj1 188 10 virus virus NN cord-264359-m9j3pcj1 188 11 ( ( -LRB- cord-264359-m9j3pcj1 188 12 Adam Adam NNP cord-264359-m9j3pcj1 188 13 and and CC cord-264359-m9j3pcj1 188 14 Strohmaier Strohmaier NNP cord-264359-m9j3pcj1 188 15 , , , cord-264359-m9j3pcj1 188 16 1974 1974 CD cord-264359-m9j3pcj1 188 17 ; ; : cord-264359-m9j3pcj1 189 1 Matheka Matheka NNP cord-264359-m9j3pcj1 189 2 and and CC cord-264359-m9j3pcj1 189 3 Bachrach Bachrach NNP cord-264359-m9j3pcj1 189 4 , , , cord-264359-m9j3pcj1 189 5 1975 1975 CD cord-264359-m9j3pcj1 189 6 ) ) -RRB- cord-264359-m9j3pcj1 189 7 , , , cord-264359-m9j3pcj1 189 8 except except IN cord-264359-m9j3pcj1 189 9 that that DT cord-264359-m9j3pcj1 189 10 in in IN cord-264359-m9j3pcj1 189 11 an an DT cord-264359-m9j3pcj1 189 12 earlier early JJR cord-264359-m9j3pcj1 189 13 paper paper NN cord-264359-m9j3pcj1 190 1 ( ( -LRB- cord-264359-m9j3pcj1 190 2 Bachrach Bachrach NNP cord-264359-m9j3pcj1 190 3 et et NNP cord-264359-m9j3pcj1 190 4 al al NNP cord-264359-m9j3pcj1 190 5 . . . cord-264359-m9j3pcj1 191 1 , , , cord-264359-m9j3pcj1 191 2 1973 1973 CD cord-264359-m9j3pcj1 191 3 ) ) -RRB- cord-264359-m9j3pcj1 191 4 an an DT cord-264359-m9j3pcj1 191 5 ambiguity ambiguity NN cord-264359-m9j3pcj1 191 6 in in IN cord-264359-m9j3pcj1 191 7 the the DT cord-264359-m9j3pcj1 191 8 C c NN cord-264359-m9j3pcj1 191 9 - - HYPH cord-264359-m9j3pcj1 191 10 terminal terminal NN cord-264359-m9j3pcj1 191 11 sequence sequence NN cord-264359-m9j3pcj1 191 12 of of IN cord-264359-m9j3pcj1 191 13 VP2 vp2 NN cord-264359-m9j3pcj1 191 14 was be VBD cord-264359-m9j3pcj1 191 15 reported report VBN cord-264359-m9j3pcj1 191 16 . . . cord-264359-m9j3pcj1 192 1 Until until IN cord-264359-m9j3pcj1 192 2 and and CC cord-264359-m9j3pcj1 192 3 unless unless IN cord-264359-m9j3pcj1 192 4 ambiguity ambiguity NN cord-264359-m9j3pcj1 192 5 of of IN cord-264359-m9j3pcj1 192 6 cleavage cleavage NN cord-264359-m9j3pcj1 192 7 is be VBZ cord-264359-m9j3pcj1 192 8 demonstrated demonstrate VBN cord-264359-m9j3pcj1 192 9 by by IN cord-264359-m9j3pcj1 192 10 means mean NNS cord-264359-m9j3pcj1 192 11 of of IN cord-264359-m9j3pcj1 192 12 sequence sequence NN cord-264359-m9j3pcj1 192 13 analysis analysis NN cord-264359-m9j3pcj1 192 14 , , , cord-264359-m9j3pcj1 192 15 other other JJ cord-264359-m9j3pcj1 192 16 possible possible JJ cord-264359-m9j3pcj1 192 17 causes cause NNS cord-264359-m9j3pcj1 192 18 of of IN cord-264359-m9j3pcj1 192 19 microheterogeneity microheterogeneity NN cord-264359-m9j3pcj1 192 20 , , , cord-264359-m9j3pcj1 192 21 such such JJ cord-264359-m9j3pcj1 192 22 as as IN cord-264359-m9j3pcj1 192 23 phosphorylation phosphorylation NN cord-264359-m9j3pcj1 192 24 , , , cord-264359-m9j3pcj1 192 25 deamidation deamidation NN cord-264359-m9j3pcj1 192 26 , , , cord-264359-m9j3pcj1 192 27 and and CC cord-264359-m9j3pcj1 192 28 glycosylation glycosylation NN cord-264359-m9j3pcj1 192 29 can can MD cord-264359-m9j3pcj1 192 30 not not RB cord-264359-m9j3pcj1 192 31 safely safely RB cord-264359-m9j3pcj1 192 32 be be VB cord-264359-m9j3pcj1 192 33 ruled rule VBN cord-264359-m9j3pcj1 192 34 out out RP cord-264359-m9j3pcj1 192 35 . . . cord-264359-m9j3pcj1 193 1 Isolation isolation NN cord-264359-m9j3pcj1 193 2 of of IN cord-264359-m9j3pcj1 193 3 the the DT cord-264359-m9j3pcj1 193 4 coat coat NN cord-264359-m9j3pcj1 193 5 proteins protein NNS cord-264359-m9j3pcj1 193 6 of of IN cord-264359-m9j3pcj1 193 7 foot foot NN cord-264359-m9j3pcj1 193 8 - - HYPH cord-264359-m9j3pcj1 193 9 and and CC cord-264359-m9j3pcj1 193 10 - - HYPH cord-264359-m9j3pcj1 193 11 mouth mouth NN cord-264359-m9j3pcj1 193 12 disease disease NN cord-264359-m9j3pcj1 193 13 virus virus NN cord-264359-m9j3pcj1 193 14 and and CC cord-264359-m9j3pcj1 193 15 analysis analysis NN cord-264359-m9j3pcj1 193 16 of of IN cord-264359-m9j3pcj1 193 17 the the DT cord-264359-m9j3pcj1 193 18 composition composition NN cord-264359-m9j3pcj1 193 19 and and CC cord-264359-m9j3pcj1 193 20 N n CD cord-264359-m9j3pcj1 193 21 - - HYPH cord-264359-m9j3pcj1 193 22 terminal terminal JJ cord-264359-m9j3pcj1 193 23 end end NN cord-264359-m9j3pcj1 193 24 groups group NNS cord-264359-m9j3pcj1 193 25 Isolation Isolation NNP cord-264359-m9j3pcj1 193 26 of of IN cord-264359-m9j3pcj1 193 27 the the DT cord-264359-m9j3pcj1 193 28 structural structural JJ cord-264359-m9j3pcj1 193 29 polypeptides polypeptide NNS cord-264359-m9j3pcj1 193 30 of of IN cord-264359-m9j3pcj1 193 31 foot foot NN cord-264359-m9j3pcj1 193 32 - - HYPH cord-264359-m9j3pcj1 193 33 and and CC cord-264359-m9j3pcj1 193 34 - - HYPH cord-264359-m9j3pcj1 193 35 mouth mouth NN cord-264359-m9j3pcj1 193 36 disease disease NN cord-264359-m9j3pcj1 193 37 virus virus NN cord-264359-m9j3pcj1 193 38 and and CC cord-264359-m9j3pcj1 193 39 analysis analysis NN cord-264359-m9j3pcj1 193 40 of of IN cord-264359-m9j3pcj1 193 41 their -PRON- PRP$ cord-264359-m9j3pcj1 193 42 C c NN cord-264359-m9j3pcj1 193 43 - - HYPH cord-264359-m9j3pcj1 193 44 terminal terminal NN cord-264359-m9j3pcj1 193 45 sequences sequence NNS cord-264359-m9j3pcj1 193 46 Cleavage cleavage NN cord-264359-m9j3pcj1 193 47 site site NN cord-264359-m9j3pcj1 193 48 alterations alteration NNS cord-264359-m9j3pcj1 193 49 in in IN cord-264359-m9j3pcj1 193 50 poliovirus poliovirus NN cord-264359-m9j3pcj1 193 51 - - HYPH cord-264359-m9j3pcj1 193 52 specific specific JJ cord-264359-m9j3pcj1 193 53 precursor precursor NN cord-264359-m9j3pcj1 193 54 proteins protein NNS cord-264359-m9j3pcj1 194 1 Molecular molecular JJ cord-264359-m9j3pcj1 194 2 weight weight NN cord-264359-m9j3pcj1 194 3 of of IN cord-264359-m9j3pcj1 194 4 a a DT cord-264359-m9j3pcj1 194 5 poliovirus poliovirus NN cord-264359-m9j3pcj1 194 6 protein protein NN cord-264359-m9j3pcj1 194 7 Picornaviral Picornaviral NNP cord-264359-m9j3pcj1 194 8 structure structure NN cord-264359-m9j3pcj1 194 9 and and CC cord-264359-m9j3pcj1 194 10 protein protein NN cord-264359-m9j3pcj1 194 11 synthesis synthesis NN cord-264359-m9j3pcj1 194 12 Evidence evidence NN cord-264359-m9j3pcj1 194 13 for for IN cord-264359-m9j3pcj1 194 14 ambiguity ambiguity NN cord-264359-m9j3pcj1 194 15 in in IN cord-264359-m9j3pcj1 194 16 the the DT cord-264359-m9j3pcj1 194 17 posttranslational posttranslational JJ cord-264359-m9j3pcj1 194 18 cleavage cleavage NN cord-264359-m9j3pcj1 194 19 of of IN cord-264359-m9j3pcj1 194 20 poliovirus poliovirus NN cord-264359-m9j3pcj1 194 21 proteins protein NNS cord-264359-m9j3pcj1 194 22 Polypeptide Polypeptide NNP cord-264359-m9j3pcj1 194 23 cleavages cleavage NNS cord-264359-m9j3pcj1 194 24 in in IN cord-264359-m9j3pcj1 194 25 the the DT cord-264359-m9j3pcj1 194 26 formation formation NN cord-264359-m9j3pcj1 194 27 of of IN cord-264359-m9j3pcj1 194 28 poliovirus poliovirus NN cord-264359-m9j3pcj1 194 29 proteins protein NNS cord-264359-m9j3pcj1 194 30 Cleavage cleavage NN cord-264359-m9j3pcj1 194 31 of of IN cord-264359-m9j3pcj1 194 32 structural structural JJ cord-264359-m9j3pcj1 194 33 proteins protein NNS cord-264359-m9j3pcj1 194 34 during during IN cord-264359-m9j3pcj1 194 35 the the DT cord-264359-m9j3pcj1 194 36 assembly assembly NN cord-264359-m9j3pcj1 194 37 of of IN cord-264359-m9j3pcj1 194 38 the the DT cord-264359-m9j3pcj1 194 39 head head NN cord-264359-m9j3pcj1 194 40 of of IN cord-264359-m9j3pcj1 194 41 bacteriophage bacteriophage NN cord-264359-m9j3pcj1 194 42 T4 T4 NNP cord-264359-m9j3pcj1 194 43 Composition Composition NNP cord-264359-m9j3pcj1 194 44 of of IN cord-264359-m9j3pcj1 194 45 artificially artificially RB cord-264359-m9j3pcj1 194 46 produced produce VBN cord-264359-m9j3pcj1 194 47 and and CC cord-264359-m9j3pcj1 194 48 naturally naturally RB cord-264359-m9j3pcj1 194 49 occurring occur VBG cord-264359-m9j3pcj1 194 50 empty empty JJ cord-264359-m9j3pcj1 194 51 capsids capsid NNS cord-264359-m9j3pcj1 194 52 of of IN cord-264359-m9j3pcj1 194 53 poliovirus poliovirus NN cord-264359-m9j3pcj1 194 54 type type NN cord-264359-m9j3pcj1 194 55 1 1 CD cord-264359-m9j3pcj1 194 56 Nterminal Nterminal NNP cord-264359-m9j3pcj1 194 57 amino amino JJ cord-264359-m9j3pcj1 194 58 acids acid NNS cord-264359-m9j3pcj1 194 59 of of IN cord-264359-m9j3pcj1 194 60 foot foot NN cord-264359-m9j3pcj1 194 61 - - HYPH cord-264359-m9j3pcj1 194 62 and and CC cord-264359-m9j3pcj1 194 63 - - HYPH cord-264359-m9j3pcj1 194 64 mouth mouth NN cord-264359-m9j3pcj1 194 65 disease disease NN cord-264359-m9j3pcj1 194 66 virus virus NN cord-264359-m9j3pcj1 194 67 peptides peptide NNS cord-264359-m9j3pcj1 194 68 Polypeptide Polypeptide NNP cord-264359-m9j3pcj1 194 69 composition composition NN cord-264359-m9j3pcj1 194 70 of of IN cord-264359-m9j3pcj1 194 71 poliovirions poliovirion NNS cord-264359-m9j3pcj1 194 72 , , , cord-264359-m9j3pcj1 194 73 naturally naturally RB cord-264359-m9j3pcj1 194 74 occurring occur VBG cord-264359-m9j3pcj1 194 75 empty empty JJ cord-264359-m9j3pcj1 194 76 capsids capsid NNS cord-264359-m9j3pcj1 194 77 , , , cord-264359-m9j3pcj1 194 78 and and CC cord-264359-m9j3pcj1 194 79 14 14 CD cord-264359-m9j3pcj1 194 80 S s NN cord-264359-m9j3pcj1 194 81 precursor precursor NN cord-264359-m9j3pcj1 194 82 particles particle NNS cord-264359-m9j3pcj1 195 1 Gene gene NN cord-264359-m9j3pcj1 195 2 order order NN cord-264359-m9j3pcj1 195 3 of of IN cord-264359-m9j3pcj1 195 4 the the DT cord-264359-m9j3pcj1 195 5 poliovirus poliovirus NN cord-264359-m9j3pcj1 195 6 capsid capsid NN cord-264359-m9j3pcj1 195 7 proteins protein VBZ cord-264359-m9j3pcj1 195 8 Electrophoretie Electrophoretie NNP cord-264359-m9j3pcj1 195 9 behavior behavior NN cord-264359-m9j3pcj1 195 10 of of IN cord-264359-m9j3pcj1 195 11 protein protein NN cord-264359-m9j3pcj1 195 12 dodecyl dodecyl NNP cord-264359-m9j3pcj1 195 13 sulfate sulfate NN cord-264359-m9j3pcj1 195 14 complexes complex NNS cord-264359-m9j3pcj1 195 15 in in IN cord-264359-m9j3pcj1 195 16 the the DT cord-264359-m9j3pcj1 195 17 presence presence NN cord-264359-m9j3pcj1 195 18 of of IN cord-264359-m9j3pcj1 195 19 various various JJ cord-264359-m9j3pcj1 195 20 amounts amount NNS cord-264359-m9j3pcj1 195 21 of of IN cord-264359-m9j3pcj1 195 22 sodium sodium NN cord-264359-m9j3pcj1 195 23 dodecyl dodecyl NNP cord-264359-m9j3pcj1 195 24 sulfate sulfate NN cord-264359-m9j3pcj1 195 25 Characterization characterization NN cord-264359-m9j3pcj1 195 26 of of IN cord-264359-m9j3pcj1 195 27 a a DT cord-264359-m9j3pcj1 195 28 coronavirus coronavirus NN cord-264359-m9j3pcj1 195 29 : : : cord-264359-m9j3pcj1 195 30 I -PRON- PRP cord-264359-m9j3pcj1 195 31 , , , cord-264359-m9j3pcj1 195 32 Structural structural JJ cord-264359-m9j3pcj1 195 33 proteins protein NNS cord-264359-m9j3pcj1 195 34 : : : cord-264359-m9j3pcj1 195 35 Effects effect NNS cord-264359-m9j3pcj1 195 36 of of IN cord-264359-m9j3pcj1 195 37 preparative preparative JJ cord-264359-m9j3pcj1 195 38 conditions condition NNS cord-264359-m9j3pcj1 195 39 on on IN cord-264359-m9j3pcj1 195 40 the the DT cord-264359-m9j3pcj1 195 41 migration migration NN cord-264359-m9j3pcj1 195 42 of of IN cord-264359-m9j3pcj1 195 43 protein protein NN cord-264359-m9j3pcj1 195 44 in in IN cord-264359-m9j3pcj1 195 45 polyacrylamide polyacrylamide NN cord-264359-m9j3pcj1 195 46 gels gel NNS cord-264359-m9j3pcj1 195 47 Evidence evidence NN cord-264359-m9j3pcj1 195 48 for for IN cord-264359-m9j3pcj1 195 49 large large JJ cord-264359-m9j3pcj1 195 50 precursor precursor NN cord-264359-m9j3pcj1 195 51 proteins protein NNS cord-264359-m9j3pcj1 195 52 in in IN cord-264359-m9j3pcj1 195 53 poliovirus poliovirus NN cord-264359-m9j3pcj1 195 54 synthesis synthesis NN cord-264359-m9j3pcj1 195 55 New new JJ cord-264359-m9j3pcj1 195 56 polypeptides polypeptide NNS cord-264359-m9j3pcj1 195 57 in in IN cord-264359-m9j3pcj1 195 58 poliovirus poliovirus NN cord-264359-m9j3pcj1 195 59 Stepwise Stepwise NNP cord-264359-m9j3pcj1 195 60 degradation degradation NN cord-264359-m9j3pcj1 195 61 of of IN cord-264359-m9j3pcj1 195 62 poliovirus poliovirus NN cord-264359-m9j3pcj1 195 63 and and CC cord-264359-m9j3pcj1 195 64 top top JJ cord-264359-m9j3pcj1 195 65 component component NN cord-264359-m9j3pcj1 195 66 by by IN cord-264359-m9j3pcj1 195 67 concentrated concentrated JJ cord-264359-m9j3pcj1 195 68 urea urea NN cord-264359-m9j3pcj1 195 69 Gel gel NN cord-264359-m9j3pcj1 195 70 electrophoresis electrophoresis NN cord-264359-m9j3pcj1 195 71 of of IN cord-264359-m9j3pcj1 195 72 protein protein NN cord-264359-m9j3pcj1 195 73 - - HYPH cord-264359-m9j3pcj1 195 74 dodecyl dodecyl JJ cord-264359-m9j3pcj1 195 75 sulfate sulfate NN cord-264359-m9j3pcj1 195 76 complexes complex NNS cord-264359-m9j3pcj1 195 77 in in IN cord-264359-m9j3pcj1 195 78 a a DT cord-264359-m9j3pcj1 195 79 pH ph NN cord-264359-m9j3pcj1 195 80 gradient gradient NN cord-264359-m9j3pcj1 195 81 and and CC cord-264359-m9j3pcj1 195 82 improved improved JJ cord-264359-m9j3pcj1 195 83 resolution resolution NN cord-264359-m9j3pcj1 195 84 of of IN cord-264359-m9j3pcj1 195 85 poliovirus poliovirus NN cord-264359-m9j3pcj1 195 86 polypeptides polypeptide NNS cord-264359-m9j3pcj1 195 87 Structure Structure NNP cord-264359-m9j3pcj1 195 88 of of IN cord-264359-m9j3pcj1 195 89 the the DT cord-264359-m9j3pcj1 195 90 Mengo Mengo NNP cord-264359-m9j3pcj1 195 91 virion virion NN cord-264359-m9j3pcj1 195 92 : : : cord-264359-m9j3pcj1 195 93 IV IV NNP cord-264359-m9j3pcj1 195 94 , , , cord-264359-m9j3pcj1 195 95 Amino amino NN cord-264359-m9j3pcj1 195 96 - - HYPH cord-264359-m9j3pcj1 195 97 and and CC cord-264359-m9j3pcj1 195 98 carboxyl carboxyl NN cord-264359-m9j3pcj1 195 99 - - HYPH cord-264359-m9j3pcj1 195 100 terminal terminal NN cord-264359-m9j3pcj1 195 101 analysis analysis NN cord-264359-m9j3pcj1 195 102 of of IN cord-264359-m9j3pcj1 195 103 the the DT cord-264359-m9j3pcj1 195 104 major major JJ cord-264359-m9j3pcj1 195 105 capsid capsid NN cord-264359-m9j3pcj1 195 106 polypeptides polypeptide NNS cord-264359-m9j3pcj1 195 107 We -PRON- PRP cord-264359-m9j3pcj1 195 108 are be VBP cord-264359-m9j3pcj1 195 109 grateful grateful JJ cord-264359-m9j3pcj1 195 110 for for IN cord-264359-m9j3pcj1 195 111 the the DT cord-264359-m9j3pcj1 195 112 excellent excellent JJ cord-264359-m9j3pcj1 195 113 technical technical JJ cord-264359-m9j3pcj1 195 114 assistance assistance NN cord-264359-m9j3pcj1 195 115 of of IN cord-264359-m9j3pcj1 195 116 A. a. NN cord-264359-m9j3pcj1 195 117 De De NNP cord-264359-m9j3pcj1 195 118 Rees Rees NNP cord-264359-m9j3pcj1 195 119 . . . cord-264359-m9j3pcj1 196 1 This this DT cord-264359-m9j3pcj1 196 2 research research NN cord-264359-m9j3pcj1 196 3 was be VBD cord-264359-m9j3pcj1 196 4 supported support VBN cord-264359-m9j3pcj1 196 5 by by IN cord-264359-m9j3pcj1 196 6 the the DT cord-264359-m9j3pcj1 196 7 Fonds Fonds NNP cord-264359-m9j3pcj1 196 8 voor voor NN cord-264359-m9j3pcj1 196 9 Kollektief Kollektief NNP cord-264359-m9j3pcj1 196 10 Fundamenteel Fundamenteel NNP cord-264359-m9j3pcj1 196 11 Onderzoek Onderzoek NNP cord-264359-m9j3pcj1 196 12 ( ( -LRB- cord-264359-m9j3pcj1 196 13 Belgium Belgium NNP cord-264359-m9j3pcj1 196 14 ) ) -RRB- cord-264359-m9j3pcj1 196 15 . . .