id sid tid token lemma pos cord-266018-8bhnlsgy 1 1 key key NN cord-266018-8bhnlsgy 1 2 : : : cord-266018-8bhnlsgy 2 1 cord-266018 cord-266018 NNP cord-266018-8bhnlsgy 2 2 - - HYPH cord-266018-8bhnlsgy 2 3 8bhnlsgy 8bhnlsgy CD cord-266018-8bhnlsgy 2 4 authors author NNS cord-266018-8bhnlsgy 2 5 : : : cord-266018-8bhnlsgy 2 6 Trifilo Trifilo NNP cord-266018-8bhnlsgy 2 7 , , , cord-266018-8bhnlsgy 2 8 Matthew Matthew NNP cord-266018-8bhnlsgy 2 9 J. J. NNP cord-266018-8bhnlsgy 2 10 ; ; : cord-266018-8bhnlsgy 2 11 Lane Lane NNP cord-266018-8bhnlsgy 2 12 , , , cord-266018-8bhnlsgy 3 1 Thomas Thomas NNP cord-266018-8bhnlsgy 3 2 E. E. NNP cord-266018-8bhnlsgy 3 3 title title NN cord-266018-8bhnlsgy 3 4 : : : cord-266018-8bhnlsgy 4 1 The the DT cord-266018-8bhnlsgy 4 2 CC cc NN cord-266018-8bhnlsgy 4 3 chemokine chemokine NN cord-266018-8bhnlsgy 4 4 ligand ligand NN cord-266018-8bhnlsgy 4 5 3 3 CD cord-266018-8bhnlsgy 4 6 regulates regulate VBZ cord-266018-8bhnlsgy 4 7 CD11c(+)CD11b(+)CD8α(− CD11c(+)CD11b(+)CD8α(− NNP cord-266018-8bhnlsgy 4 8 ) ) -RRB- cord-266018-8bhnlsgy 4 9 dendritic dendritic JJ cord-266018-8bhnlsgy 4 10 cell cell NN cord-266018-8bhnlsgy 4 11 maturation maturation NN cord-266018-8bhnlsgy 4 12 and and CC cord-266018-8bhnlsgy 4 13 activation activation NN cord-266018-8bhnlsgy 4 14 following follow VBG cord-266018-8bhnlsgy 4 15 viral viral JJ cord-266018-8bhnlsgy 4 16 infection infection NN cord-266018-8bhnlsgy 4 17 of of IN cord-266018-8bhnlsgy 4 18 the the DT cord-266018-8bhnlsgy 4 19 central central JJ cord-266018-8bhnlsgy 4 20 nervous nervous JJ cord-266018-8bhnlsgy 4 21 system system NN cord-266018-8bhnlsgy 4 22 : : : cord-266018-8bhnlsgy 4 23 implications implication NNS cord-266018-8bhnlsgy 4 24 for for IN cord-266018-8bhnlsgy 4 25 a a DT cord-266018-8bhnlsgy 4 26 role role NN cord-266018-8bhnlsgy 4 27 in in IN cord-266018-8bhnlsgy 4 28 T T NNP cord-266018-8bhnlsgy 4 29 cell cell NN cord-266018-8bhnlsgy 4 30 activation activation NN cord-266018-8bhnlsgy 4 31 date date NN cord-266018-8bhnlsgy 4 32 : : : cord-266018-8bhnlsgy 4 33 2004 2004 CD cord-266018-8bhnlsgy 4 34 - - SYM cord-266018-8bhnlsgy 4 35 09 09 CD cord-266018-8bhnlsgy 4 36 - - SYM cord-266018-8bhnlsgy 4 37 15 15 CD cord-266018-8bhnlsgy 4 38 journal journal NN cord-266018-8bhnlsgy 4 39 : : : cord-266018-8bhnlsgy 4 40 Virology Virology NNP cord-266018-8bhnlsgy 4 41 DOI DOI NNP cord-266018-8bhnlsgy 4 42 : : : cord-266018-8bhnlsgy 5 1 10.1016/ 10.1016/ CD cord-266018-8bhnlsgy 6 1 j.virol.2004.06.027 j.virol.2004.06.027 NNP cord-266018-8bhnlsgy 7 1 sha sha NNP cord-266018-8bhnlsgy 8 1 : : : cord-266018-8bhnlsgy 8 2 2461bbb3482eeb061c49c461ea54f5ae17c0926a 2461bbb3482eeb061c49c461ea54f5ae17c0926a CD cord-266018-8bhnlsgy 8 3 doc_id doc_id CD cord-266018-8bhnlsgy 8 4 : : : cord-266018-8bhnlsgy 9 1 266018 266018 CD cord-266018-8bhnlsgy 9 2 cord_uid cord_uid NNS cord-266018-8bhnlsgy 9 3 : : : cord-266018-8bhnlsgy 10 1 8bhnlsgy 8bhnlsgy CD cord-266018-8bhnlsgy 11 1 The the DT cord-266018-8bhnlsgy 11 2 role role NN cord-266018-8bhnlsgy 11 3 of of IN cord-266018-8bhnlsgy 11 4 CC cc NN cord-266018-8bhnlsgy 11 5 chemokine chemokine NN cord-266018-8bhnlsgy 11 6 ligand ligand NN cord-266018-8bhnlsgy 11 7 3 3 CD cord-266018-8bhnlsgy 11 8 ( ( -LRB- cord-266018-8bhnlsgy 11 9 CCL3 CCL3 NNP cord-266018-8bhnlsgy 11 10 ) ) -RRB- cord-266018-8bhnlsgy 11 11 in in IN cord-266018-8bhnlsgy 11 12 activation activation NN cord-266018-8bhnlsgy 11 13 of of IN cord-266018-8bhnlsgy 11 14 dendritic dendritic JJ cord-266018-8bhnlsgy 11 15 cells cell NNS cord-266018-8bhnlsgy 11 16 ( ( -LRB- cord-266018-8bhnlsgy 11 17 DCs DCs NNP cord-266018-8bhnlsgy 11 18 ) ) -RRB- cord-266018-8bhnlsgy 11 19 following follow VBG cord-266018-8bhnlsgy 11 20 mouse mouse NN cord-266018-8bhnlsgy 11 21 hepatitis hepatitis NN cord-266018-8bhnlsgy 11 22 virus virus NN cord-266018-8bhnlsgy 11 23 ( ( -LRB- cord-266018-8bhnlsgy 11 24 MHV mhv NN cord-266018-8bhnlsgy 11 25 ) ) -RRB- cord-266018-8bhnlsgy 11 26 infection infection NN cord-266018-8bhnlsgy 11 27 of of IN cord-266018-8bhnlsgy 11 28 the the DT cord-266018-8bhnlsgy 11 29 central central JJ cord-266018-8bhnlsgy 11 30 nervous nervous JJ cord-266018-8bhnlsgy 11 31 system system NN cord-266018-8bhnlsgy 11 32 ( ( -LRB- cord-266018-8bhnlsgy 11 33 CNS CNS NNP cord-266018-8bhnlsgy 11 34 ) ) -RRB- cord-266018-8bhnlsgy 11 35 was be VBD cord-266018-8bhnlsgy 11 36 examined examine VBN cord-266018-8bhnlsgy 11 37 . . . cord-266018-8bhnlsgy 12 1 The the DT cord-266018-8bhnlsgy 12 2 results result NNS cord-266018-8bhnlsgy 12 3 indicate indicate VBP cord-266018-8bhnlsgy 12 4 that that IN cord-266018-8bhnlsgy 12 5 CCL3 CCL3 NNP cord-266018-8bhnlsgy 12 6 participates participate VBZ cord-266018-8bhnlsgy 12 7 in in IN cord-266018-8bhnlsgy 12 8 an an DT cord-266018-8bhnlsgy 12 9 effective effective JJ cord-266018-8bhnlsgy 12 10 host host NN cord-266018-8bhnlsgy 12 11 response response NN cord-266018-8bhnlsgy 12 12 to to IN cord-266018-8bhnlsgy 12 13 MHV MHV NNP cord-266018-8bhnlsgy 12 14 infection infection NN cord-266018-8bhnlsgy 12 15 by by IN cord-266018-8bhnlsgy 12 16 contributing contribute VBG cord-266018-8bhnlsgy 12 17 to to IN cord-266018-8bhnlsgy 12 18 CD11c(+)CD11b(+)CD8α(− CD11c(+)CD11b(+)CD8α(− NNP cord-266018-8bhnlsgy 12 19 ) ) -RRB- cord-266018-8bhnlsgy 13 1 DC DC NNP cord-266018-8bhnlsgy 13 2 maturation maturation NN cord-266018-8bhnlsgy 13 3 , , , cord-266018-8bhnlsgy 13 4 activation activation NN cord-266018-8bhnlsgy 13 5 , , , cord-266018-8bhnlsgy 13 6 and and CC cord-266018-8bhnlsgy 13 7 migration migration NN cord-266018-8bhnlsgy 13 8 to to IN cord-266018-8bhnlsgy 13 9 cervical cervical JJ cord-266018-8bhnlsgy 13 10 lymph lymph NN cord-266018-8bhnlsgy 13 11 nodes node NNS cord-266018-8bhnlsgy 13 12 ( ( -LRB- cord-266018-8bhnlsgy 13 13 CLN CLN NNP cord-266018-8bhnlsgy 13 14 ) ) -RRB- cord-266018-8bhnlsgy 13 15 . . . cord-266018-8bhnlsgy 14 1 Diminished diminished NN cord-266018-8bhnlsgy 14 2 CD8α(− CD8α(− NNS cord-266018-8bhnlsgy 14 3 ) ) -RRB- cord-266018-8bhnlsgy 15 1 DC DC NNP cord-266018-8bhnlsgy 15 2 activation activation NN cord-266018-8bhnlsgy 15 3 correlated correlate VBN cord-266018-8bhnlsgy 15 4 with with IN cord-266018-8bhnlsgy 15 5 reduced reduce VBN cord-266018-8bhnlsgy 15 6 IFN IFN NNP cord-266018-8bhnlsgy 15 7 - - HYPH cord-266018-8bhnlsgy 15 8 γ γ NN cord-266018-8bhnlsgy 15 9 expression expression NN cord-266018-8bhnlsgy 15 10 by by IN cord-266018-8bhnlsgy 15 11 virus virus NN cord-266018-8bhnlsgy 15 12 - - HYPH cord-266018-8bhnlsgy 15 13 specific specific JJ cord-266018-8bhnlsgy 15 14 T t NN cord-266018-8bhnlsgy 15 15 cells cell NNS cord-266018-8bhnlsgy 15 16 accompanied accompany VBN cord-266018-8bhnlsgy 15 17 by by IN cord-266018-8bhnlsgy 15 18 increased increase VBN cord-266018-8bhnlsgy 15 19 IL-10 IL-10 NNP cord-266018-8bhnlsgy 15 20 production production NN cord-266018-8bhnlsgy 15 21 suggesting suggest VBG cord-266018-8bhnlsgy 15 22 that that IN cord-266018-8bhnlsgy 15 23 CCL3 CCL3 NNP cord-266018-8bhnlsgy 15 24 contributes contribute VBZ cord-266018-8bhnlsgy 15 25 to to IN cord-266018-8bhnlsgy 15 26 an an DT cord-266018-8bhnlsgy 15 27 effective effective JJ cord-266018-8bhnlsgy 15 28 host host NN cord-266018-8bhnlsgy 15 29 response response NN cord-266018-8bhnlsgy 15 30 to to IN cord-266018-8bhnlsgy 15 31 viral viral JJ cord-266018-8bhnlsgy 15 32 infection infection NN cord-266018-8bhnlsgy 15 33 by by IN cord-266018-8bhnlsgy 15 34 enhancing enhance VBG cord-266018-8bhnlsgy 15 35 the the DT cord-266018-8bhnlsgy 15 36 T T NNP cord-266018-8bhnlsgy 15 37 cell cell NN cord-266018-8bhnlsgy 15 38 activation activation NN cord-266018-8bhnlsgy 15 39 potential potential NN cord-266018-8bhnlsgy 15 40 of of IN cord-266018-8bhnlsgy 15 41 DC DC NNP cord-266018-8bhnlsgy 15 42 . . . cord-266018-8bhnlsgy 16 1 The the DT cord-266018-8bhnlsgy 16 2 CC cc NN cord-266018-8bhnlsgy 16 3 chemokine chemokine NN cord-266018-8bhnlsgy 16 4 ligand ligand NN cord-266018-8bhnlsgy 16 5 3 3 CD cord-266018-8bhnlsgy 17 1 ( ( -LRB- cord-266018-8bhnlsgy 17 2 CCL3-macrophage ccl3-macrophage NN cord-266018-8bhnlsgy 17 3 inflammatory inflammatory JJ cord-266018-8bhnlsgy 17 4 protein-1a protein-1a NNP cord-266018-8bhnlsgy 17 5 ) ) -RRB- cord-266018-8bhnlsgy 17 6 is be VBZ cord-266018-8bhnlsgy 17 7 capable capable JJ cord-266018-8bhnlsgy 17 8 of of IN cord-266018-8bhnlsgy 17 9 activating activate VBG cord-266018-8bhnlsgy 17 10 monocytes monocyte NNS cord-266018-8bhnlsgy 17 11 and and CC cord-266018-8bhnlsgy 17 12 lymphocytes lymphocyte NNS cord-266018-8bhnlsgy 17 13 and and CC cord-266018-8bhnlsgy 17 14 serves serve VBZ cord-266018-8bhnlsgy 17 15 an an DT cord-266018-8bhnlsgy 17 16 important important JJ cord-266018-8bhnlsgy 17 17 role role NN cord-266018-8bhnlsgy 17 18 in in IN cord-266018-8bhnlsgy 17 19 the the DT cord-266018-8bhnlsgy 17 20 initial initial JJ cord-266018-8bhnlsgy 17 21 recruitment recruitment NN cord-266018-8bhnlsgy 17 22 of of IN cord-266018-8bhnlsgy 17 23 these these DT cord-266018-8bhnlsgy 17 24 cells cell NNS cord-266018-8bhnlsgy 17 25 to to IN cord-266018-8bhnlsgy 17 26 tissues tissue NNS cord-266018-8bhnlsgy 17 27 following follow VBG cord-266018-8bhnlsgy 17 28 microbial microbial JJ cord-266018-8bhnlsgy 17 29 infection infection NN cord-266018-8bhnlsgy 18 1 ( ( -LRB- cord-266018-8bhnlsgy 18 2 Cook Cook NNP cord-266018-8bhnlsgy 18 3 et et NNP cord-266018-8bhnlsgy 18 4 al al NNP cord-266018-8bhnlsgy 18 5 . . . cord-266018-8bhnlsgy 19 1 , , , cord-266018-8bhnlsgy 19 2 1995 1995 CD cord-266018-8bhnlsgy 19 3 ; ; : cord-266018-8bhnlsgy 19 4 Domachowske Domachowske NNP cord-266018-8bhnlsgy 19 5 et et FW cord-266018-8bhnlsgy 19 6 al al NNP cord-266018-8bhnlsgy 19 7 . . NNP cord-266018-8bhnlsgy 19 8 , , , cord-266018-8bhnlsgy 19 9 2000 2000 CD cord-266018-8bhnlsgy 19 10 ) ) -RRB- cord-266018-8bhnlsgy 19 11 . . . cord-266018-8bhnlsgy 20 1 In in IN cord-266018-8bhnlsgy 20 2 support support NN cord-266018-8bhnlsgy 20 3 of of IN cord-266018-8bhnlsgy 20 4 the the DT cord-266018-8bhnlsgy 20 5 importance importance NN cord-266018-8bhnlsgy 20 6 for for IN cord-266018-8bhnlsgy 20 7 CCL3 CCL3 NNP cord-266018-8bhnlsgy 20 8 in in IN cord-266018-8bhnlsgy 20 9 imparting impart VBG cord-266018-8bhnlsgy 20 10 functional functional JJ cord-266018-8bhnlsgy 20 11 signals signal NNS cord-266018-8bhnlsgy 20 12 to to IN cord-266018-8bhnlsgy 20 13 T t NN cord-266018-8bhnlsgy 20 14 cells cell NNS cord-266018-8bhnlsgy 20 15 are be VBP cord-266018-8bhnlsgy 20 16 data datum NNS cord-266018-8bhnlsgy 20 17 from from IN cord-266018-8bhnlsgy 20 18 our -PRON- PRP$ cord-266018-8bhnlsgy 20 19 laboratory laboratory NN cord-266018-8bhnlsgy 20 20 demonstrating demonstrate VBG cord-266018-8bhnlsgy 20 21 that that DT cord-266018-8bhnlsgy 20 22 instillation instillation NN cord-266018-8bhnlsgy 20 23 of of IN cord-266018-8bhnlsgy 20 24 mouse mouse NNP cord-266018-8bhnlsgy 20 25 hepatitis hepatitis NNP cord-266018-8bhnlsgy 20 26 virus virus NN cord-266018-8bhnlsgy 20 27 ( ( -LRB- cord-266018-8bhnlsgy 20 28 MHV MHV NNP cord-266018-8bhnlsgy 20 29 ) ) -RRB- cord-266018-8bhnlsgy 20 30 into into IN cord-266018-8bhnlsgy 20 31 the the DT cord-266018-8bhnlsgy 20 32 brains brain NNS cord-266018-8bhnlsgy 20 33 of of IN cord-266018-8bhnlsgy 20 34 CCL3 CCL3 NNP cord-266018-8bhnlsgy 20 35 À à NN cord-266018-8bhnlsgy 20 36 / / SYM cord-266018-8bhnlsgy 20 37 À à NN cord-266018-8bhnlsgy 20 38 mice mice NN cord-266018-8bhnlsgy 20 39 results result VBZ cord-266018-8bhnlsgy 20 40 in in IN cord-266018-8bhnlsgy 20 41 an an DT cord-266018-8bhnlsgy 20 42 inability inability NN cord-266018-8bhnlsgy 20 43 to to TO cord-266018-8bhnlsgy 20 44 clear clear VB cord-266018-8bhnlsgy 20 45 virus virus NN cord-266018-8bhnlsgy 20 46 from from IN cord-266018-8bhnlsgy 20 47 the the DT cord-266018-8bhnlsgy 20 48 central central JJ cord-266018-8bhnlsgy 20 49 nervous nervous JJ cord-266018-8bhnlsgy 20 50 system system NN cord-266018-8bhnlsgy 20 51 ( ( -LRB- cord-266018-8bhnlsgy 20 52 CNS CNS NNP cord-266018-8bhnlsgy 20 53 ) ) -RRB- cord-266018-8bhnlsgy 21 1 ( ( -LRB- cord-266018-8bhnlsgy 21 2 Trifilo Trifilo NNP cord-266018-8bhnlsgy 21 3 et et FW cord-266018-8bhnlsgy 21 4 al al NNP cord-266018-8bhnlsgy 21 5 . . NNP cord-266018-8bhnlsgy 21 6 , , , cord-266018-8bhnlsgy 21 7 2003 2003 CD cord-266018-8bhnlsgy 21 8 ) ) -RRB- cord-266018-8bhnlsgy 21 9 . . . cord-266018-8bhnlsgy 22 1 MHV MHV NNP cord-266018-8bhnlsgy 22 2 - - HYPH cord-266018-8bhnlsgy 22 3 infected infect VBN cord-266018-8bhnlsgy 22 4 CCL3 CCL3 NNP cord-266018-8bhnlsgy 22 5 À à NN cord-266018-8bhnlsgy 22 6 / / SYM cord-266018-8bhnlsgy 22 7 À à NN cord-266018-8bhnlsgy 22 8 mice mouse NNS cord-266018-8bhnlsgy 22 9 exhibited exhibit VBD cord-266018-8bhnlsgy 22 10 a a DT cord-266018-8bhnlsgy 22 11 significant significant JJ cord-266018-8bhnlsgy 22 12 reduction reduction NN cord-266018-8bhnlsgy 22 13 in in IN cord-266018-8bhnlsgy 22 14 the the DT cord-266018-8bhnlsgy 22 15 numbers number NNS cord-266018-8bhnlsgy 22 16 of of IN cord-266018-8bhnlsgy 22 17 infiltrating infiltrate VBG cord-266018-8bhnlsgy 22 18 virus virus NN cord-266018-8bhnlsgy 22 19 - - HYPH cord-266018-8bhnlsgy 22 20 specific specific JJ cord-266018-8bhnlsgy 22 21 CD8 CD8 NNP cord-266018-8bhnlsgy 22 22 + + CC cord-266018-8bhnlsgy 22 23 T t NN cord-266018-8bhnlsgy 22 24 cells cell NNS cord-266018-8bhnlsgy 22 25 present present JJ cord-266018-8bhnlsgy 22 26 within within IN cord-266018-8bhnlsgy 22 27 the the DT cord-266018-8bhnlsgy 22 28 brain brain NN cord-266018-8bhnlsgy 22 29 indicating indicate VBG cord-266018-8bhnlsgy 22 30 that that IN cord-266018-8bhnlsgy 22 31 trafficking trafficking NN cord-266018-8bhnlsgy 22 32 was be VBD cord-266018-8bhnlsgy 22 33 impaired impair VBN cord-266018-8bhnlsgy 22 34 . . . cord-266018-8bhnlsgy 23 1 Moreover moreover RB cord-266018-8bhnlsgy 23 2 , , , cord-266018-8bhnlsgy 23 3 the the DT cord-266018-8bhnlsgy 23 4 ability ability NN cord-266018-8bhnlsgy 23 5 to to TO cord-266018-8bhnlsgy 23 6 produce produce VB cord-266018-8bhnlsgy 23 7 IFN IFN NNP cord-266018-8bhnlsgy 23 8 - - HYPH cord-266018-8bhnlsgy 23 9 g g NNP cord-266018-8bhnlsgy 23 10 as as RB cord-266018-8bhnlsgy 23 11 well well RB cord-266018-8bhnlsgy 23 12 as as IN cord-266018-8bhnlsgy 23 13 the the DT cord-266018-8bhnlsgy 23 14 cytolytic cytolytic JJ cord-266018-8bhnlsgy 23 15 activity activity NN cord-266018-8bhnlsgy 23 16 of of IN cord-266018-8bhnlsgy 23 17 virus virus NN cord-266018-8bhnlsgy 23 18 - - HYPH cord-266018-8bhnlsgy 23 19 specific specific JJ cord-266018-8bhnlsgy 23 20 CD8 CD8 NNP cord-266018-8bhnlsgy 23 21 + + CC cord-266018-8bhnlsgy 23 22 T t NN cord-266018-8bhnlsgy 23 23 cells cell NNS cord-266018-8bhnlsgy 23 24 was be VBD cord-266018-8bhnlsgy 23 25 dramatically dramatically RB cord-266018-8bhnlsgy 23 26 reduced reduce VBN cord-266018-8bhnlsgy 23 27 in in IN cord-266018-8bhnlsgy 23 28 the the DT cord-266018-8bhnlsgy 23 29 absence absence NN cord-266018-8bhnlsgy 23 30 of of IN cord-266018-8bhnlsgy 23 31 CCL3 CCL3 NNP cord-266018-8bhnlsgy 23 32 signaling signaling NN cord-266018-8bhnlsgy 23 33 . . . cord-266018-8bhnlsgy 24 1 Taken take VBN cord-266018-8bhnlsgy 24 2 together together RB cord-266018-8bhnlsgy 24 3 , , , cord-266018-8bhnlsgy 24 4 these these DT cord-266018-8bhnlsgy 24 5 data datum NNS cord-266018-8bhnlsgy 24 6 indicate indicate VBP cord-266018-8bhnlsgy 24 7 that that IN cord-266018-8bhnlsgy 24 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 24 9 signaling signal VBG cord-266018-8bhnlsgy 24 10 significantly significantly RB cord-266018-8bhnlsgy 24 11 enhances enhance VBZ cord-266018-8bhnlsgy 24 12 the the DT cord-266018-8bhnlsgy 24 13 differentiation differentiation NN cord-266018-8bhnlsgy 24 14 of of IN cord-266018-8bhnlsgy 24 15 primed prime VBN cord-266018-8bhnlsgy 24 16 CD8 CD8 NNP cord-266018-8bhnlsgy 24 17 + + CC cord-266018-8bhnlsgy 24 18 T T NNP cord-266018-8bhnlsgy 24 19 cells cell NNS cord-266018-8bhnlsgy 24 20 into into IN cord-266018-8bhnlsgy 24 21 effector effector NN cord-266018-8bhnlsgy 24 22 cells cell NNS cord-266018-8bhnlsgy 24 23 that that WDT cord-266018-8bhnlsgy 24 24 allows allow VBZ cord-266018-8bhnlsgy 24 25 their -PRON- PRP$ cord-266018-8bhnlsgy 24 26 release release NN cord-266018-8bhnlsgy 24 27 from from IN cord-266018-8bhnlsgy 24 28 secondary secondary JJ cord-266018-8bhnlsgy 24 29 lymphoid lymphoid JJ cord-266018-8bhnlsgy 24 30 organs organ NNS cord-266018-8bhnlsgy 24 31 into into IN cord-266018-8bhnlsgy 24 32 circulation circulation NN cord-266018-8bhnlsgy 24 33 and and CC cord-266018-8bhnlsgy 24 34 effective effective JJ cord-266018-8bhnlsgy 24 35 migration migration NN cord-266018-8bhnlsgy 24 36 to to IN cord-266018-8bhnlsgy 24 37 the the DT cord-266018-8bhnlsgy 24 38 CNS CNS NNP cord-266018-8bhnlsgy 24 39 . . . cord-266018-8bhnlsgy 25 1 The the DT cord-266018-8bhnlsgy 25 2 present present JJ cord-266018-8bhnlsgy 25 3 study study NN cord-266018-8bhnlsgy 25 4 was be VBD cord-266018-8bhnlsgy 25 5 undertaken undertake VBN cord-266018-8bhnlsgy 25 6 to to TO cord-266018-8bhnlsgy 25 7 characterize characterize VB cord-266018-8bhnlsgy 25 8 potential potential JJ cord-266018-8bhnlsgy 25 9 mechanisms mechanism NNS cord-266018-8bhnlsgy 25 10 by by IN cord-266018-8bhnlsgy 25 11 which which WDT cord-266018-8bhnlsgy 25 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 25 13 signaling signaling NN cord-266018-8bhnlsgy 25 14 imparts impart VBZ cord-266018-8bhnlsgy 25 15 effector effector JJ cord-266018-8bhnlsgy 25 16 function function NN cord-266018-8bhnlsgy 25 17 to to IN cord-266018-8bhnlsgy 25 18 antigen antigen NN cord-266018-8bhnlsgy 25 19 - - HYPH cord-266018-8bhnlsgy 25 20 specific specific JJ cord-266018-8bhnlsgy 25 21 T t NN cord-266018-8bhnlsgy 25 22 cells cell NNS cord-266018-8bhnlsgy 25 23 following follow VBG cord-266018-8bhnlsgy 25 24 MHV MHV NNP cord-266018-8bhnlsgy 25 25 infection infection NN cord-266018-8bhnlsgy 25 26 of of IN cord-266018-8bhnlsgy 25 27 the the DT cord-266018-8bhnlsgy 25 28 CNS CNS NNP cord-266018-8bhnlsgy 25 29 . . . cord-266018-8bhnlsgy 26 1 To to TO cord-266018-8bhnlsgy 26 2 further further RB cord-266018-8bhnlsgy 26 3 understand understand VB cord-266018-8bhnlsgy 26 4 the the DT cord-266018-8bhnlsgy 26 5 relationship relationship NN cord-266018-8bhnlsgy 26 6 between between IN cord-266018-8bhnlsgy 26 7 CCL3 CCL3 NNP cord-266018-8bhnlsgy 26 8 signaling signaling NN cord-266018-8bhnlsgy 26 9 and and CC cord-266018-8bhnlsgy 26 10 T T NNP cord-266018-8bhnlsgy 26 11 cell cell NN cord-266018-8bhnlsgy 26 12 activation activation NN cord-266018-8bhnlsgy 26 13 , , , cord-266018-8bhnlsgy 26 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 26 15 + + CC cord-266018-8bhnlsgy 26 16 /+ /+ . cord-266018-8bhnlsgy 26 17 and and CC cord-266018-8bhnlsgy 26 18 CCL3 CCL3 NNP cord-266018-8bhnlsgy 26 19 À À NNP cord-266018-8bhnlsgy 26 20 / / SYM cord-266018-8bhnlsgy 26 21 À à NN cord-266018-8bhnlsgy 26 22 mice mouse NNS cord-266018-8bhnlsgy 26 23 were be VBD cord-266018-8bhnlsgy 26 24 infected infect VBN cord-266018-8bhnlsgy 26 25 with with IN cord-266018-8bhnlsgy 26 26 MHV MHV NNP cord-266018-8bhnlsgy 26 27 and and CC cord-266018-8bhnlsgy 26 28 the the DT cord-266018-8bhnlsgy 26 29 presence presence NN cord-266018-8bhnlsgy 26 30 and and CC cord-266018-8bhnlsgy 26 31 activation activation NN cord-266018-8bhnlsgy 26 32 state state NN cord-266018-8bhnlsgy 26 33 of of IN cord-266018-8bhnlsgy 26 34 DC DC NNP cord-266018-8bhnlsgy 26 35 - - HYPH cord-266018-8bhnlsgy 26 36 like like JJ cord-266018-8bhnlsgy 26 37 cells cell NNS cord-266018-8bhnlsgy 26 38 within within IN cord-266018-8bhnlsgy 26 39 the the DT cord-266018-8bhnlsgy 26 40 brain brain NN cord-266018-8bhnlsgy 26 41 and and CC cord-266018-8bhnlsgy 26 42 draining drain VBG cord-266018-8bhnlsgy 26 43 cervical cervical JJ cord-266018-8bhnlsgy 26 44 lymph lymph NN cord-266018-8bhnlsgy 26 45 nodes node NNS cord-266018-8bhnlsgy 26 46 ( ( -LRB- cord-266018-8bhnlsgy 26 47 CLN CLN NNP cord-266018-8bhnlsgy 26 48 ) ) -RRB- cord-266018-8bhnlsgy 26 49 determined determine VBD cord-266018-8bhnlsgy 26 50 . . . cord-266018-8bhnlsgy 27 1 Our -PRON- PRP$ cord-266018-8bhnlsgy 27 2 results result NNS cord-266018-8bhnlsgy 27 3 delineate delineate VBP cord-266018-8bhnlsgy 27 4 a a DT cord-266018-8bhnlsgy 27 5 CCL3-dependent CCL3-dependent NNP cord-266018-8bhnlsgy 27 6 pathway pathway NN cord-266018-8bhnlsgy 27 7 of of IN cord-266018-8bhnlsgy 27 8 T T NNP cord-266018-8bhnlsgy 27 9 cell cell NN cord-266018-8bhnlsgy 27 10 activation activation NN cord-266018-8bhnlsgy 27 11 that that WDT cord-266018-8bhnlsgy 27 12 involves involve VBZ cord-266018-8bhnlsgy 27 13 the the DT cord-266018-8bhnlsgy 27 14 maturation maturation NN cord-266018-8bhnlsgy 27 15 and and CC cord-266018-8bhnlsgy 27 16 activation activation NN cord-266018-8bhnlsgy 27 17 of of IN cord-266018-8bhnlsgy 27 18 a a DT cord-266018-8bhnlsgy 27 19 subpopulation subpopulation NN cord-266018-8bhnlsgy 27 20 of of IN cord-266018-8bhnlsgy 27 21 ( ( -LRB- cord-266018-8bhnlsgy 27 22 dendritic dendritic JJ cord-266018-8bhnlsgy 27 23 cells cell NNS cord-266018-8bhnlsgy 27 24 ) ) -RRB- cord-266018-8bhnlsgy 27 25 DCs DCs NNP cord-266018-8bhnlsgy 27 26 ( ( -LRB- cord-266018-8bhnlsgy 27 27 CD11c CD11c NNP cord-266018-8bhnlsgy 27 28 + + SYM cord-266018-8bhnlsgy 27 29 CD11b CD11b NNS cord-266018-8bhnlsgy 27 30 + + CC cord-266018-8bhnlsgy 27 31 CD8a CD8a NNP cord-266018-8bhnlsgy 27 32 À À NNP cord-266018-8bhnlsgy 27 33 ) ) -RRB- cord-266018-8bhnlsgy 27 34 within within IN cord-266018-8bhnlsgy 27 35 the the DT cord-266018-8bhnlsgy 27 36 CNS CNS NNP cord-266018-8bhnlsgy 27 37 as as RB cord-266018-8bhnlsgy 27 38 well well RB cord-266018-8bhnlsgy 27 39 as as IN cord-266018-8bhnlsgy 27 40 influencing influence VBG cord-266018-8bhnlsgy 27 41 the the DT cord-266018-8bhnlsgy 27 42 accumulation accumulation NN cord-266018-8bhnlsgy 27 43 of of IN cord-266018-8bhnlsgy 27 44 these these DT cord-266018-8bhnlsgy 27 45 cells cell NNS cord-266018-8bhnlsgy 27 46 within within IN cord-266018-8bhnlsgy 27 47 the the DT cord-266018-8bhnlsgy 27 48 CLN cln NN cord-266018-8bhnlsgy 27 49 following follow VBG cord-266018-8bhnlsgy 27 50 MHV MHV NNP cord-266018-8bhnlsgy 27 51 infection infection NN cord-266018-8bhnlsgy 27 52 of of IN cord-266018-8bhnlsgy 27 53 the the DT cord-266018-8bhnlsgy 27 54 CNS CNS NNP cord-266018-8bhnlsgy 27 55 . . . cord-266018-8bhnlsgy 27 56 _SP cord-266018-8bhnlsgy 28 1 Characterization characterization NN cord-266018-8bhnlsgy 28 2 of of IN cord-266018-8bhnlsgy 28 3 CD11c CD11c NNP cord-266018-8bhnlsgy 28 4 + + CC cord-266018-8bhnlsgy 28 5 cells cell NNS cord-266018-8bhnlsgy 28 6 within within IN cord-266018-8bhnlsgy 28 7 the the DT cord-266018-8bhnlsgy 28 8 CNS CNS NNP cord-266018-8bhnlsgy 28 9 following follow VBG cord-266018-8bhnlsgy 28 10 MHV MHV NNP cord-266018-8bhnlsgy 28 11 infection infection NN cord-266018-8bhnlsgy 28 12 of of IN cord-266018-8bhnlsgy 28 13 CCL3 CCL3 NNP cord-266018-8bhnlsgy 28 14 + + CC cord-266018-8bhnlsgy 29 1 /+ /+ . cord-266018-8bhnlsgy 30 1 and and CC cord-266018-8bhnlsgy 30 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 30 3 À À NNP cord-266018-8bhnlsgy 30 4 / / SYM cord-266018-8bhnlsgy 30 5 À à NN cord-266018-8bhnlsgy 30 6 mice mouse NNS cord-266018-8bhnlsgy 31 1 To to TO cord-266018-8bhnlsgy 31 2 characterize characterize VB cord-266018-8bhnlsgy 31 3 the the DT cord-266018-8bhnlsgy 31 4 populations population NNS cord-266018-8bhnlsgy 31 5 of of IN cord-266018-8bhnlsgy 31 6 cells cell NNS cord-266018-8bhnlsgy 31 7 present present JJ cord-266018-8bhnlsgy 31 8 within within IN cord-266018-8bhnlsgy 31 9 the the DT cord-266018-8bhnlsgy 31 10 CNS CNS NNP cord-266018-8bhnlsgy 31 11 of of IN cord-266018-8bhnlsgy 31 12 MHV MHV NNP cord-266018-8bhnlsgy 31 13 - - HYPH cord-266018-8bhnlsgy 31 14 infected infect VBN cord-266018-8bhnlsgy 31 15 mice mouse NNS cord-266018-8bhnlsgy 31 16 , , , cord-266018-8bhnlsgy 31 17 cells cell NNS cord-266018-8bhnlsgy 31 18 were be VBD cord-266018-8bhnlsgy 31 19 harvested harvest VBN cord-266018-8bhnlsgy 31 20 at at IN cord-266018-8bhnlsgy 31 21 days day NNS cord-266018-8bhnlsgy 31 22 0 0 CD cord-266018-8bhnlsgy 31 23 , , , cord-266018-8bhnlsgy 31 24 2 2 CD cord-266018-8bhnlsgy 31 25 , , , cord-266018-8bhnlsgy 31 26 and and CC cord-266018-8bhnlsgy 31 27 5 5 CD cord-266018-8bhnlsgy 31 28 post post NN cord-266018-8bhnlsgy 31 29 - - NN cord-266018-8bhnlsgy 31 30 infection infection JJ cord-266018-8bhnlsgy 31 31 ( ( -LRB- cord-266018-8bhnlsgy 31 32 p.i p.i NNP cord-266018-8bhnlsgy 31 33 . . NNP cord-266018-8bhnlsgy 31 34 ) ) -RRB- cord-266018-8bhnlsgy 31 35 and and CC cord-266018-8bhnlsgy 31 36 immunophenotyped immunophenotype VBN cord-266018-8bhnlsgy 31 37 by by IN cord-266018-8bhnlsgy 31 38 flow flow NN cord-266018-8bhnlsgy 31 39 cytometry cytometry NN cord-266018-8bhnlsgy 31 40 . . . cord-266018-8bhnlsgy 32 1 We -PRON- PRP cord-266018-8bhnlsgy 32 2 chose choose VBD cord-266018-8bhnlsgy 32 3 to to TO cord-266018-8bhnlsgy 32 4 focus focus VB cord-266018-8bhnlsgy 32 5 our -PRON- PRP$ cord-266018-8bhnlsgy 32 6 attention attention NN cord-266018-8bhnlsgy 32 7 on on IN cord-266018-8bhnlsgy 32 8 markers marker NNS cord-266018-8bhnlsgy 32 9 that that WDT cord-266018-8bhnlsgy 32 10 are be VBP cord-266018-8bhnlsgy 32 11 associated associate VBN cord-266018-8bhnlsgy 32 12 with with IN cord-266018-8bhnlsgy 32 13 professional professional JJ cord-266018-8bhnlsgy 32 14 antigen antigen NN cord-266018-8bhnlsgy 32 15 presenting present VBG cord-266018-8bhnlsgy 32 16 cells cell NNS cord-266018-8bhnlsgy 32 17 such such JJ cord-266018-8bhnlsgy 32 18 as as IN cord-266018-8bhnlsgy 32 19 DC DC NNP cord-266018-8bhnlsgy 32 20 as as IN cord-266018-8bhnlsgy 32 21 recent recent JJ cord-266018-8bhnlsgy 32 22 studies study NNS cord-266018-8bhnlsgy 32 23 have have VBP cord-266018-8bhnlsgy 32 24 indicated indicate VBN cord-266018-8bhnlsgy 32 25 DC DC NNP cord-266018-8bhnlsgy 32 26 - - HYPH cord-266018-8bhnlsgy 32 27 like like JJ cord-266018-8bhnlsgy 32 28 cells cell NNS cord-266018-8bhnlsgy 32 29 can can MD cord-266018-8bhnlsgy 32 30 be be VB cord-266018-8bhnlsgy 32 31 detected detect VBN cord-266018-8bhnlsgy 32 32 within within IN cord-266018-8bhnlsgy 32 33 the the DT cord-266018-8bhnlsgy 32 34 brains brain NNS cord-266018-8bhnlsgy 32 35 under under IN cord-266018-8bhnlsgy 32 36 inflammatory inflammatory JJ cord-266018-8bhnlsgy 32 37 conditions condition NNS cord-266018-8bhnlsgy 32 38 ( ( -LRB- cord-266018-8bhnlsgy 32 39 Fisher Fisher NNP cord-266018-8bhnlsgy 32 40 and and CC cord-266018-8bhnlsgy 32 41 Reichmann Reichmann NNP cord-266018-8bhnlsgy 32 42 , , , cord-266018-8bhnlsgy 32 43 2001 2001 CD cord-266018-8bhnlsgy 32 44 ; ; : cord-266018-8bhnlsgy 32 45 Fischer Fischer NNP cord-266018-8bhnlsgy 32 46 et et NNP cord-266018-8bhnlsgy 32 47 al al NNP cord-266018-8bhnlsgy 32 48 . . NNP cord-266018-8bhnlsgy 32 49 , , , cord-266018-8bhnlsgy 32 50 2000 2000 CD cord-266018-8bhnlsgy 32 51 ) ) -RRB- cord-266018-8bhnlsgy 32 52 . . . cord-266018-8bhnlsgy 33 1 Furthermore furthermore RB cord-266018-8bhnlsgy 33 2 , , , cord-266018-8bhnlsgy 33 3 we -PRON- PRP cord-266018-8bhnlsgy 33 4 have have VBP cord-266018-8bhnlsgy 33 5 previously previously RB cord-266018-8bhnlsgy 33 6 determined determine VBN cord-266018-8bhnlsgy 33 7 that that IN cord-266018-8bhnlsgy 33 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 33 9 mRNA mRNA NNP cord-266018-8bhnlsgy 33 10 expression expression NN cord-266018-8bhnlsgy 33 11 is be VBZ cord-266018-8bhnlsgy 33 12 detected detect VBN cord-266018-8bhnlsgy 33 13 within within IN cord-266018-8bhnlsgy 33 14 the the DT cord-266018-8bhnlsgy 33 15 CNS CNS NNP cord-266018-8bhnlsgy 33 16 early early RB cord-266018-8bhnlsgy 33 17 ( ( -LRB- cord-266018-8bhnlsgy 33 18 b3 b3 NN cord-266018-8bhnlsgy 33 19 days day NNS cord-266018-8bhnlsgy 33 20 ) ) -RRB- cord-266018-8bhnlsgy 33 21 and and CC cord-266018-8bhnlsgy 33 22 therefore therefore RB cord-266018-8bhnlsgy 33 23 may may MD cord-266018-8bhnlsgy 33 24 participate participate VB cord-266018-8bhnlsgy 33 25 in in IN cord-266018-8bhnlsgy 33 26 the the DT cord-266018-8bhnlsgy 33 27 appearance appearance NN cord-266018-8bhnlsgy 33 28 of of IN cord-266018-8bhnlsgy 33 29 CD11c CD11c NNP cord-266018-8bhnlsgy 33 30 + + CC cord-266018-8bhnlsgy 33 31 cells cell NNS cord-266018-8bhnlsgy 33 32 within within IN cord-266018-8bhnlsgy 33 33 the the DT cord-266018-8bhnlsgy 33 34 brain brain NN cord-266018-8bhnlsgy 33 35 following follow VBG cord-266018-8bhnlsgy 33 36 MHV MHV NNP cord-266018-8bhnlsgy 33 37 infection infection NN cord-266018-8bhnlsgy 34 1 ( ( -LRB- cord-266018-8bhnlsgy 34 2 Trifilo Trifilo NNP cord-266018-8bhnlsgy 34 3 et et FW cord-266018-8bhnlsgy 34 4 al al NNP cord-266018-8bhnlsgy 34 5 . . NNP cord-266018-8bhnlsgy 34 6 , , , cord-266018-8bhnlsgy 34 7 2003 2003 CD cord-266018-8bhnlsgy 34 8 ) ) -RRB- cord-266018-8bhnlsgy 34 9 . . . cord-266018-8bhnlsgy 35 1 Therefore therefore RB cord-266018-8bhnlsgy 35 2 , , , cord-266018-8bhnlsgy 35 3 we -PRON- PRP cord-266018-8bhnlsgy 35 4 sought seek VBD cord-266018-8bhnlsgy 35 5 to to TO cord-266018-8bhnlsgy 35 6 characterize characterize VB cord-266018-8bhnlsgy 35 7 the the DT cord-266018-8bhnlsgy 35 8 populations population NNS cord-266018-8bhnlsgy 35 9 of of IN cord-266018-8bhnlsgy 35 10 CD11c CD11c NNP cord-266018-8bhnlsgy 35 11 + + CC cord-266018-8bhnlsgy 35 12 cells cell NNS cord-266018-8bhnlsgy 35 13 within within IN cord-266018-8bhnlsgy 35 14 the the DT cord-266018-8bhnlsgy 35 15 brain brain NN cord-266018-8bhnlsgy 35 16 following follow VBG cord-266018-8bhnlsgy 35 17 MHV MHV NNP cord-266018-8bhnlsgy 35 18 infection infection NN cord-266018-8bhnlsgy 35 19 of of IN cord-266018-8bhnlsgy 35 20 CCL3 CCL3 NNP cord-266018-8bhnlsgy 35 21 + + CC cord-266018-8bhnlsgy 36 1 /+ /+ . cord-266018-8bhnlsgy 37 1 and and CC cord-266018-8bhnlsgy 37 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 37 3 À À NNP cord-266018-8bhnlsgy 37 4 / / SYM cord-266018-8bhnlsgy 37 5 À à NN cord-266018-8bhnlsgy 37 6 mice mouse NNS cord-266018-8bhnlsgy 37 7 . . . cord-266018-8bhnlsgy 38 1 Results result NNS cord-266018-8bhnlsgy 38 2 in in IN cord-266018-8bhnlsgy 38 3 Fig Fig NNP cord-266018-8bhnlsgy 38 4 . . . cord-266018-8bhnlsgy 39 1 1A 1A NNP cord-266018-8bhnlsgy 39 2 indicate indicate VBP cord-266018-8bhnlsgy 39 3 the the DT cord-266018-8bhnlsgy 39 4 frequency frequency NN cord-266018-8bhnlsgy 39 5 of of IN cord-266018-8bhnlsgy 39 6 CD11c CD11c NNP cord-266018-8bhnlsgy 39 7 + + CC cord-266018-8bhnlsgy 39 8 cells cell NNS cord-266018-8bhnlsgy 39 9 present present JJ cord-266018-8bhnlsgy 39 10 within within IN cord-266018-8bhnlsgy 39 11 the the DT cord-266018-8bhnlsgy 39 12 CNS CNS NNP cord-266018-8bhnlsgy 39 13 of of IN cord-266018-8bhnlsgy 39 14 naRve naRve NNP cord-266018-8bhnlsgy 39 15 CCL3 CCL3 NNP cord-266018-8bhnlsgy 39 16 + + CC cord-266018-8bhnlsgy 39 17 /+ /+ . cord-266018-8bhnlsgy 39 18 and and CC cord-266018-8bhnlsgy 39 19 CCL3 CCL3 NNP cord-266018-8bhnlsgy 39 20 À À NNP cord-266018-8bhnlsgy 39 21 / / SYM cord-266018-8bhnlsgy 39 22 À à NN cord-266018-8bhnlsgy 39 23 mice mice NN cord-266018-8bhnlsgy 39 24 is be VBZ cord-266018-8bhnlsgy 39 25 b1 b1 NN cord-266018-8bhnlsgy 39 26 % % NN cord-266018-8bhnlsgy 39 27 . . . cord-266018-8bhnlsgy 40 1 However however RB cord-266018-8bhnlsgy 40 2 , , , cord-266018-8bhnlsgy 40 3 within within IN cord-266018-8bhnlsgy 40 4 2 2 CD cord-266018-8bhnlsgy 40 5 days day NNS cord-266018-8bhnlsgy 40 6 following follow VBG cord-266018-8bhnlsgy 40 7 intracranial intracranial JJ cord-266018-8bhnlsgy 40 8 infection infection NN cord-266018-8bhnlsgy 40 9 with with IN cord-266018-8bhnlsgy 40 10 MHV MHV NNP cord-266018-8bhnlsgy 40 11 , , , cord-266018-8bhnlsgy 40 12 there there EX cord-266018-8bhnlsgy 40 13 is be VBZ cord-266018-8bhnlsgy 40 14 a a DT cord-266018-8bhnlsgy 40 15 marked marked JJ cord-266018-8bhnlsgy 40 16 increase increase NN cord-266018-8bhnlsgy 40 17 in in IN cord-266018-8bhnlsgy 40 18 the the DT cord-266018-8bhnlsgy 40 19 frequency frequency NN cord-266018-8bhnlsgy 40 20 of of IN cord-266018-8bhnlsgy 40 21 CD11c CD11c NNP cord-266018-8bhnlsgy 40 22 + + CC cord-266018-8bhnlsgy 40 23 cells cell NNS cord-266018-8bhnlsgy 40 24 within within IN cord-266018-8bhnlsgy 40 25 the the DT cord-266018-8bhnlsgy 40 26 brains brain NNS cord-266018-8bhnlsgy 40 27 of of IN cord-266018-8bhnlsgy 40 28 both both DT cord-266018-8bhnlsgy 40 29 strains strain NNS cord-266018-8bhnlsgy 40 30 of of IN cord-266018-8bhnlsgy 40 31 mice mouse NNS cord-266018-8bhnlsgy 40 32 ( ( -LRB- cord-266018-8bhnlsgy 40 33 Fig Fig NNP cord-266018-8bhnlsgy 40 34 . . . cord-266018-8bhnlsgy 40 35 1A 1a NN cord-266018-8bhnlsgy 40 36 ) ) -RRB- cord-266018-8bhnlsgy 40 37 . . . cord-266018-8bhnlsgy 41 1 Analysis analysis NN cord-266018-8bhnlsgy 41 2 of of IN cord-266018-8bhnlsgy 41 3 CD11b CD11b NNPS cord-266018-8bhnlsgy 41 4 expression expression NN cord-266018-8bhnlsgy 41 5 revealed reveal VBD cord-266018-8bhnlsgy 41 6 that that IN cord-266018-8bhnlsgy 41 7 approximately approximately RB cord-266018-8bhnlsgy 41 8 75 75 CD cord-266018-8bhnlsgy 41 9 % % NN cord-266018-8bhnlsgy 41 10 of of IN cord-266018-8bhnlsgy 41 11 CD11c CD11c NNP cord-266018-8bhnlsgy 41 12 + + CC cord-266018-8bhnlsgy 41 13 cells cell NNS cord-266018-8bhnlsgy 41 14 in in IN cord-266018-8bhnlsgy 41 15 both both CC cord-266018-8bhnlsgy 41 16 CCL3 CCL3 NNP cord-266018-8bhnlsgy 41 17 + + CC cord-266018-8bhnlsgy 42 1 /+ /+ . cord-266018-8bhnlsgy 43 1 and and CC cord-266018-8bhnlsgy 43 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 43 3 À À NNP cord-266018-8bhnlsgy 43 4 / / SYM cord-266018-8bhnlsgy 43 5 À à NN cord-266018-8bhnlsgy 43 6 mice mouse NNS cord-266018-8bhnlsgy 43 7 were be VBD cord-266018-8bhnlsgy 43 8 also also RB cord-266018-8bhnlsgy 43 9 CD11b CD11b NNS cord-266018-8bhnlsgy 43 10 + + CD cord-266018-8bhnlsgy 43 11 ( ( -LRB- cord-266018-8bhnlsgy 43 12 Fig Fig NNP cord-266018-8bhnlsgy 43 13 . . . cord-266018-8bhnlsgy 43 14 1A 1a NN cord-266018-8bhnlsgy 43 15 ) ) -RRB- cord-266018-8bhnlsgy 43 16 . . . cord-266018-8bhnlsgy 44 1 CD11c CD11c NNP cord-266018-8bhnlsgy 44 2 + + SYM cord-266018-8bhnlsgy 44 3 CD11b CD11b NNS cord-266018-8bhnlsgy 45 1 + + CC cord-266018-8bhnlsgy 45 2 cells cell NNS cord-266018-8bhnlsgy 45 3 isolated isolate VBN cord-266018-8bhnlsgy 45 4 from from IN cord-266018-8bhnlsgy 45 5 the the DT cord-266018-8bhnlsgy 45 6 CNS CNS NNP cord-266018-8bhnlsgy 45 7 of of IN cord-266018-8bhnlsgy 45 8 either either DT cord-266018-8bhnlsgy 45 9 CCL3 CCL3 NNP cord-266018-8bhnlsgy 45 10 + + CD cord-266018-8bhnlsgy 46 1 /+ /+ NFP cord-266018-8bhnlsgy 46 2 or or CC cord-266018-8bhnlsgy 47 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 47 2 À à NN cord-266018-8bhnlsgy 47 3 / / SYM cord-266018-8bhnlsgy 47 4 À à NN cord-266018-8bhnlsgy 47 5 mice mouse NNS cord-266018-8bhnlsgy 47 6 expressed express VBD cord-266018-8bhnlsgy 47 7 little little JJ cord-266018-8bhnlsgy 47 8 to to IN cord-266018-8bhnlsgy 47 9 no no DT cord-266018-8bhnlsgy 47 10 CD8a CD8a NNP cord-266018-8bhnlsgy 47 11 or or CC cord-266018-8bhnlsgy 47 12 DEC205 DEC205 NNP cord-266018-8bhnlsgy 47 13 suggesting suggest VBG cord-266018-8bhnlsgy 47 14 a a DT cord-266018-8bhnlsgy 47 15 phenotype phenotype NN cord-266018-8bhnlsgy 47 16 similar similar JJ cord-266018-8bhnlsgy 47 17 to to IN cord-266018-8bhnlsgy 47 18 myeloid myeloid JJ cord-266018-8bhnlsgy 47 19 derived derive VBN cord-266018-8bhnlsgy 47 20 DC DC NNP cord-266018-8bhnlsgy 47 21 ( ( -LRB- cord-266018-8bhnlsgy 47 22 CD11c CD11c NNP cord-266018-8bhnlsgy 47 23 + + CC cord-266018-8bhnlsgy 47 24 , , . cord-266018-8bhnlsgy 48 1 CD11b CD11b NNS cord-266018-8bhnlsgy 48 2 + + CC cord-266018-8bhnlsgy 48 3 , , , cord-266018-8bhnlsgy 48 4 CD8a CD8a NNP cord-266018-8bhnlsgy 48 5 À À NNP cord-266018-8bhnlsgy 48 6 , , , cord-266018-8bhnlsgy 48 7 DEC205 DEC205 NNP cord-266018-8bhnlsgy 48 8 À à NN cord-266018-8bhnlsgy 48 9 ) ) -RRB- cord-266018-8bhnlsgy 49 1 ( ( -LRB- cord-266018-8bhnlsgy 49 2 Fig Fig NNP cord-266018-8bhnlsgy 49 3 . . . cord-266018-8bhnlsgy 49 4 _SP cord-266018-8bhnlsgy 49 5 1B 1b CD cord-266018-8bhnlsgy 49 6 ) ) -RRB- cord-266018-8bhnlsgy 50 1 ( ( -LRB- cord-266018-8bhnlsgy 50 2 Anjuere Anjuere NNP cord-266018-8bhnlsgy 50 3 et et FW cord-266018-8bhnlsgy 50 4 al al NNP cord-266018-8bhnlsgy 50 5 . . . cord-266018-8bhnlsgy 51 1 , , , cord-266018-8bhnlsgy 51 2 1999 1999 CD cord-266018-8bhnlsgy 51 3 ; ; : cord-266018-8bhnlsgy 51 4 Henri Henri NNP cord-266018-8bhnlsgy 51 5 et et NNP cord-266018-8bhnlsgy 51 6 al al NNP cord-266018-8bhnlsgy 51 7 . . NNP cord-266018-8bhnlsgy 51 8 , , , cord-266018-8bhnlsgy 51 9 2001 2001 CD cord-266018-8bhnlsgy 51 10 ) ) -RRB- cord-266018-8bhnlsgy 51 11 . . . cord-266018-8bhnlsgy 52 1 Increased Increased NNP cord-266018-8bhnlsgy 52 2 CD8a CD8a NNP cord-266018-8bhnlsgy 52 3 and and CC cord-266018-8bhnlsgy 52 4 DEC205 DEC205 NNP cord-266018-8bhnlsgy 52 5 expression expression NN cord-266018-8bhnlsgy 52 6 on on IN cord-266018-8bhnlsgy 52 7 CD11c CD11c NNP cord-266018-8bhnlsgy 52 8 + + SYM cord-266018-8bhnlsgy 52 9 CD11b CD11b NNS cord-266018-8bhnlsgy 52 10 À à NN cord-266018-8bhnlsgy 52 11 cells cell NNS cord-266018-8bhnlsgy 52 12 indicated indicate VBD cord-266018-8bhnlsgy 52 13 that that IN cord-266018-8bhnlsgy 52 14 the the DT cord-266018-8bhnlsgy 52 15 majority majority NN cord-266018-8bhnlsgy 52 16 of of IN cord-266018-8bhnlsgy 52 17 the the DT cord-266018-8bhnlsgy 52 18 remaining remain VBG cord-266018-8bhnlsgy 52 19 CD11c CD11c NNP cord-266018-8bhnlsgy 52 20 + + CC cord-266018-8bhnlsgy 52 21 cells cell NNS cord-266018-8bhnlsgy 52 22 present present JJ cord-266018-8bhnlsgy 52 23 within within IN cord-266018-8bhnlsgy 52 24 the the DT cord-266018-8bhnlsgy 52 25 brain brain NN cord-266018-8bhnlsgy 52 26 were be VBD cord-266018-8bhnlsgy 52 27 similar similar JJ cord-266018-8bhnlsgy 52 28 phenotypically phenotypically RB cord-266018-8bhnlsgy 52 29 to to IN cord-266018-8bhnlsgy 52 30 lymphoid lymphoid JJ cord-266018-8bhnlsgy 52 31 derived derive VBN cord-266018-8bhnlsgy 52 32 DC DC NNP cord-266018-8bhnlsgy 52 33 ( ( -LRB- cord-266018-8bhnlsgy 52 34 CD11c CD11c NNP cord-266018-8bhnlsgy 52 35 + + CC cord-266018-8bhnlsgy 52 36 , , , cord-266018-8bhnlsgy 52 37 CD11b CD11b NNPS cord-266018-8bhnlsgy 52 38 À à NN cord-266018-8bhnlsgy 52 39 , , , cord-266018-8bhnlsgy 52 40 CD8a CD8a NNP cord-266018-8bhnlsgy 52 41 + + CC cord-266018-8bhnlsgy 52 42 ) ) -RRB- cord-266018-8bhnlsgy 53 1 ( ( -LRB- cord-266018-8bhnlsgy 53 2 Fig Fig NNP cord-266018-8bhnlsgy 53 3 . . . cord-266018-8bhnlsgy 53 4 1B 1b LS cord-266018-8bhnlsgy 53 5 ) ) -RRB- cord-266018-8bhnlsgy 54 1 ( ( -LRB- cord-266018-8bhnlsgy 54 2 Anjuere Anjuere NNP cord-266018-8bhnlsgy 54 3 et et FW cord-266018-8bhnlsgy 54 4 al al NNP cord-266018-8bhnlsgy 54 5 . . . cord-266018-8bhnlsgy 55 1 , , , cord-266018-8bhnlsgy 55 2 1999 1999 CD cord-266018-8bhnlsgy 55 3 ; ; : cord-266018-8bhnlsgy 55 4 Henri Henri NNP cord-266018-8bhnlsgy 55 5 et et NNP cord-266018-8bhnlsgy 55 6 al al NNP cord-266018-8bhnlsgy 55 7 . . NNP cord-266018-8bhnlsgy 55 8 , , , cord-266018-8bhnlsgy 55 9 2001 2001 CD cord-266018-8bhnlsgy 55 10 ) ) -RRB- cord-266018-8bhnlsgy 55 11 . . . cord-266018-8bhnlsgy 56 1 Although although IN cord-266018-8bhnlsgy 56 2 the the DT cord-266018-8bhnlsgy 56 3 Fig Fig NNP cord-266018-8bhnlsgy 56 4 . . NNP cord-266018-8bhnlsgy 56 5 1 1 CD cord-266018-8bhnlsgy 56 6 . . . cord-266018-8bhnlsgy 57 1 Analysis analysis NN cord-266018-8bhnlsgy 57 2 of of IN cord-266018-8bhnlsgy 57 3 dendritic dendritic JJ cord-266018-8bhnlsgy 57 4 cells cell NNS cord-266018-8bhnlsgy 57 5 ( ( -LRB- cord-266018-8bhnlsgy 57 6 DCs DCs NNP cord-266018-8bhnlsgy 57 7 ) ) -RRB- cord-266018-8bhnlsgy 57 8 within within IN cord-266018-8bhnlsgy 57 9 the the DT cord-266018-8bhnlsgy 57 10 brain brain NN cord-266018-8bhnlsgy 57 11 following follow VBG cord-266018-8bhnlsgy 57 12 MHV MHV NNP cord-266018-8bhnlsgy 57 13 infection infection NN cord-266018-8bhnlsgy 57 14 . . . cord-266018-8bhnlsgy 58 1 ( ( -LRB- cord-266018-8bhnlsgy 58 2 A a NN cord-266018-8bhnlsgy 58 3 ) ) -RRB- cord-266018-8bhnlsgy 58 4 Cells cell NNS cord-266018-8bhnlsgy 58 5 were be VBD cord-266018-8bhnlsgy 58 6 isolated isolate VBN cord-266018-8bhnlsgy 58 7 from from IN cord-266018-8bhnlsgy 58 8 the the DT cord-266018-8bhnlsgy 58 9 brains brain NNS cord-266018-8bhnlsgy 58 10 of of IN cord-266018-8bhnlsgy 58 11 uninfected uninfected JJ cord-266018-8bhnlsgy 58 12 ( ( -LRB- cord-266018-8bhnlsgy 58 13 naRve naRve NNP cord-266018-8bhnlsgy 58 14 ) ) -RRB- cord-266018-8bhnlsgy 58 15 or or CC cord-266018-8bhnlsgy 58 16 infected infect VBN cord-266018-8bhnlsgy 58 17 ( ( -LRB- cord-266018-8bhnlsgy 58 18 day day NN cord-266018-8bhnlsgy 58 19 2 2 CD cord-266018-8bhnlsgy 58 20 p.i p.i NNP cord-266018-8bhnlsgy 58 21 . . . cord-266018-8bhnlsgy 58 22 ) ) -RRB- cord-266018-8bhnlsgy 58 23 CCL3 CCL3 NNP cord-266018-8bhnlsgy 58 24 + + CC cord-266018-8bhnlsgy 58 25 /+ /+ . cord-266018-8bhnlsgy 58 26 and and CC cord-266018-8bhnlsgy 58 27 CCL3 CCL3 NNP cord-266018-8bhnlsgy 58 28 À À NNP cord-266018-8bhnlsgy 58 29 / / SYM cord-266018-8bhnlsgy 58 30 À à NN cord-266018-8bhnlsgy 58 31 mice mouse NNS cord-266018-8bhnlsgy 58 32 and and CC cord-266018-8bhnlsgy 58 33 stained stain VBN cord-266018-8bhnlsgy 58 34 for for IN cord-266018-8bhnlsgy 58 35 CD11c CD11c NNP cord-266018-8bhnlsgy 58 36 + + CC cord-266018-8bhnlsgy 58 37 and and CC cord-266018-8bhnlsgy 58 38 CD11b CD11b NNS cord-266018-8bhnlsgy 58 39 + + CC cord-266018-8bhnlsgy 58 40 expression expression NN cord-266018-8bhnlsgy 58 41 . . . cord-266018-8bhnlsgy 59 1 Gated gated JJ cord-266018-8bhnlsgy 59 2 populations population NNS cord-266018-8bhnlsgy 59 3 represent represent VBP cord-266018-8bhnlsgy 59 4 CD11c CD11c NNP cord-266018-8bhnlsgy 59 5 + + SYM cord-266018-8bhnlsgy 59 6 CD11b CD11b NNS cord-266018-8bhnlsgy 59 7 + + CC cord-266018-8bhnlsgy 59 8 ( ( -LRB- cord-266018-8bhnlsgy 59 9 upper upper JJ cord-266018-8bhnlsgy 59 10 - - HYPH cord-266018-8bhnlsgy 59 11 right right JJ cord-266018-8bhnlsgy 59 12 quadrant quadrant NN cord-266018-8bhnlsgy 59 13 ) ) -RRB- cord-266018-8bhnlsgy 59 14 or or CC cord-266018-8bhnlsgy 59 15 CD11c CD11c NNP cord-266018-8bhnlsgy 59 16 + + SYM cord-266018-8bhnlsgy 59 17 CD11b CD11b NNS cord-266018-8bhnlsgy 59 18 À à NN cord-266018-8bhnlsgy 59 19 ( ( -LRB- cord-266018-8bhnlsgy 59 20 lower low JJR cord-266018-8bhnlsgy 59 21 - - HYPH cord-266018-8bhnlsgy 59 22 right right JJ cord-266018-8bhnlsgy 59 23 quadrant quadrant NN cord-266018-8bhnlsgy 59 24 ) ) -RRB- cord-266018-8bhnlsgy 59 25 and and CC cord-266018-8bhnlsgy 59 26 numbers number NNS cord-266018-8bhnlsgy 59 27 indicate indicate VBP cord-266018-8bhnlsgy 59 28 frequencies frequency NNS cord-266018-8bhnlsgy 59 29 of of IN cord-266018-8bhnlsgy 59 30 gated gated JJ cord-266018-8bhnlsgy 59 31 cells cell NNS cord-266018-8bhnlsgy 59 32 within within IN cord-266018-8bhnlsgy 59 33 the the DT cord-266018-8bhnlsgy 59 34 isolated isolated JJ cord-266018-8bhnlsgy 59 35 population population NN cord-266018-8bhnlsgy 59 36 . . . cord-266018-8bhnlsgy 60 1 ( ( -LRB- cord-266018-8bhnlsgy 60 2 B b NN cord-266018-8bhnlsgy 60 3 ) ) -RRB- cord-266018-8bhnlsgy 60 4 CD8a CD8a NNP cord-266018-8bhnlsgy 60 5 and and CC cord-266018-8bhnlsgy 60 6 DEC205 DEC205 NNP cord-266018-8bhnlsgy 60 7 expression expression NN cord-266018-8bhnlsgy 60 8 on on IN cord-266018-8bhnlsgy 60 9 CD11c CD11c NNP cord-266018-8bhnlsgy 60 10 + + CC cord-266018-8bhnlsgy 60 11 cells cell NNS cord-266018-8bhnlsgy 60 12 . . . cord-266018-8bhnlsgy 61 1 CD11c CD11c NNP cord-266018-8bhnlsgy 61 2 + + SYM cord-266018-8bhnlsgy 61 3 CD11b CD11b NNS cord-266018-8bhnlsgy 61 4 + + CC cord-266018-8bhnlsgy 61 5 cells cell NNS cord-266018-8bhnlsgy 61 6 from from IN cord-266018-8bhnlsgy 61 7 either either CC cord-266018-8bhnlsgy 61 8 MHV MHV NNP cord-266018-8bhnlsgy 61 9 - - HYPH cord-266018-8bhnlsgy 61 10 infected infect VBN cord-266018-8bhnlsgy 61 11 CCL3 CCL3 NNP cord-266018-8bhnlsgy 61 12 + + CC cord-266018-8bhnlsgy 62 1 /+ /+ NFP cord-266018-8bhnlsgy 62 2 or or CC cord-266018-8bhnlsgy 63 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 63 2 À à NN cord-266018-8bhnlsgy 63 3 / / SYM cord-266018-8bhnlsgy 63 4 À à NN cord-266018-8bhnlsgy 63 5 mice mouse NNS cord-266018-8bhnlsgy 63 6 at at IN cord-266018-8bhnlsgy 63 7 day day NN cord-266018-8bhnlsgy 63 8 2 2 CD cord-266018-8bhnlsgy 63 9 p.i p.i NNP cord-266018-8bhnlsgy 63 10 . . NNP cord-266018-8bhnlsgy 63 11 did do VBD cord-266018-8bhnlsgy 63 12 not not RB cord-266018-8bhnlsgy 63 13 express express VB cord-266018-8bhnlsgy 63 14 detectable detectable JJ cord-266018-8bhnlsgy 63 15 levels level NNS cord-266018-8bhnlsgy 63 16 of of IN cord-266018-8bhnlsgy 63 17 either either CC cord-266018-8bhnlsgy 63 18 CD8a CD8a NNP cord-266018-8bhnlsgy 63 19 or or CC cord-266018-8bhnlsgy 63 20 DEC205 DEC205 NNP cord-266018-8bhnlsgy 63 21 while while IN cord-266018-8bhnlsgy 63 22 expression expression NN cord-266018-8bhnlsgy 63 23 of of IN cord-266018-8bhnlsgy 63 24 both both CC cord-266018-8bhnlsgy 63 25 CD8a CD8a NNP cord-266018-8bhnlsgy 63 26 and and CC cord-266018-8bhnlsgy 63 27 DEC205 DEC205 NNP cord-266018-8bhnlsgy 63 28 was be VBD cord-266018-8bhnlsgy 63 29 readily readily RB cord-266018-8bhnlsgy 63 30 detectable detectable JJ cord-266018-8bhnlsgy 63 31 on on IN cord-266018-8bhnlsgy 63 32 CD11c CD11c NNP cord-266018-8bhnlsgy 63 33 + + CC cord-266018-8bhnlsgy 64 1 CD11b CD11b NNS cord-266018-8bhnlsgy 64 2 À à NN cord-266018-8bhnlsgy 64 3 cells cell NNS cord-266018-8bhnlsgy 64 4 present present JJ cord-266018-8bhnlsgy 64 5 within within IN cord-266018-8bhnlsgy 64 6 the the DT cord-266018-8bhnlsgy 64 7 brains brain NNS cord-266018-8bhnlsgy 64 8 of of IN cord-266018-8bhnlsgy 64 9 both both DT cord-266018-8bhnlsgy 64 10 populations population NNS cord-266018-8bhnlsgy 64 11 of of IN cord-266018-8bhnlsgy 64 12 mice mouse NNS cord-266018-8bhnlsgy 64 13 at at IN cord-266018-8bhnlsgy 64 14 day day NN cord-266018-8bhnlsgy 65 1 2 2 CD cord-266018-8bhnlsgy 65 2 p.i p.i NNP cord-266018-8bhnlsgy 65 3 . . . cord-266018-8bhnlsgy 66 1 ( ( -LRB- cord-266018-8bhnlsgy 66 2 C C NNP cord-266018-8bhnlsgy 66 3 ) ) -RRB- cord-266018-8bhnlsgy 66 4 Total total JJ cord-266018-8bhnlsgy 66 5 numbers number NNS cord-266018-8bhnlsgy 66 6 of of IN cord-266018-8bhnlsgy 66 7 CD8a CD8a NNP cord-266018-8bhnlsgy 66 8 + + CC cord-266018-8bhnlsgy 66 9 and and CC cord-266018-8bhnlsgy 66 10 CD8a CD8a NNP cord-266018-8bhnlsgy 66 11 À À HYPH cord-266018-8bhnlsgy 66 12 DCs dc NNS cord-266018-8bhnlsgy 66 13 within within IN cord-266018-8bhnlsgy 66 14 the the DT cord-266018-8bhnlsgy 66 15 brains brain NNS cord-266018-8bhnlsgy 66 16 of of IN cord-266018-8bhnlsgy 66 17 MHV mhv NN cord-266018-8bhnlsgy 66 18 or or CC cord-266018-8bhnlsgy 66 19 sham sham NN cord-266018-8bhnlsgy 66 20 - - HYPH cord-266018-8bhnlsgy 66 21 infected infected JJ cord-266018-8bhnlsgy 66 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 66 23 + + CC cord-266018-8bhnlsgy 67 1 /+ /+ . cord-266018-8bhnlsgy 68 1 and and CC cord-266018-8bhnlsgy 68 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 68 3 À À NNP cord-266018-8bhnlsgy 68 4 / / SYM cord-266018-8bhnlsgy 68 5 À à NN cord-266018-8bhnlsgy 68 6 mice mouse NNS cord-266018-8bhnlsgy 68 7 at at IN cord-266018-8bhnlsgy 68 8 days day NNS cord-266018-8bhnlsgy 68 9 2 2 CD cord-266018-8bhnlsgy 68 10 and and CC cord-266018-8bhnlsgy 69 1 5 5 CD cord-266018-8bhnlsgy 69 2 p.i p.i NNP cord-266018-8bhnlsgy 69 3 . . NNP cord-266018-8bhnlsgy 69 4 Data Data NNP cord-266018-8bhnlsgy 69 5 presented present VBD cord-266018-8bhnlsgy 69 6 represent represent VBP cord-266018-8bhnlsgy 69 7 an an DT cord-266018-8bhnlsgy 69 8 average average JJ cord-266018-8bhnlsgy 69 9 cell cell NN cord-266018-8bhnlsgy 69 10 number number NN cord-266018-8bhnlsgy 69 11 derived derive VBN cord-266018-8bhnlsgy 69 12 from from IN cord-266018-8bhnlsgy 69 13 two two CD cord-266018-8bhnlsgy 69 14 separate separate JJ cord-266018-8bhnlsgy 69 15 experiments experiment NNS cord-266018-8bhnlsgy 69 16 with with IN cord-266018-8bhnlsgy 69 17 a a DT cord-266018-8bhnlsgy 69 18 minimum minimum NN cord-266018-8bhnlsgy 69 19 of of IN cord-266018-8bhnlsgy 69 20 10 10 CD cord-266018-8bhnlsgy 69 21 mice mouse NNS cord-266018-8bhnlsgy 69 22 analyzed analyze VBN cord-266018-8bhnlsgy 69 23 per per IN cord-266018-8bhnlsgy 69 24 experimental experimental JJ cord-266018-8bhnlsgy 69 25 group group NN cord-266018-8bhnlsgy 69 26 . . . cord-266018-8bhnlsgy 70 1 ( ( -LRB- cord-266018-8bhnlsgy 70 2 D D NNP cord-266018-8bhnlsgy 70 3 ) ) -RRB- cord-266018-8bhnlsgy 70 4 CD8a CD8a NNP cord-266018-8bhnlsgy 70 5 À à NN cord-266018-8bhnlsgy 70 6 cells cell NNS cord-266018-8bhnlsgy 70 7 isolated isolate VBN cord-266018-8bhnlsgy 70 8 from from IN cord-266018-8bhnlsgy 70 9 brains brain NNS cord-266018-8bhnlsgy 70 10 of of IN cord-266018-8bhnlsgy 70 11 CCL3 CCL3 NNP cord-266018-8bhnlsgy 70 12 + + CC cord-266018-8bhnlsgy 71 1 /+ /+ NFP cord-266018-8bhnlsgy 71 2 or or CC cord-266018-8bhnlsgy 72 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 72 2 À à NN cord-266018-8bhnlsgy 72 3 / / SYM cord-266018-8bhnlsgy 72 4 À à NN cord-266018-8bhnlsgy 72 5 mice mouse NNS cord-266018-8bhnlsgy 72 6 at at IN cord-266018-8bhnlsgy 72 7 day day NN cord-266018-8bhnlsgy 72 8 2 2 CD cord-266018-8bhnlsgy 72 9 p.i p.i NNP cord-266018-8bhnlsgy 72 10 . . NNP cord-266018-8bhnlsgy 72 11 were be VBD cord-266018-8bhnlsgy 72 12 gated gate VBN cord-266018-8bhnlsgy 72 13 upon upon IN cord-266018-8bhnlsgy 72 14 and and CC cord-266018-8bhnlsgy 72 15 CD80 CD80 NNP cord-266018-8bhnlsgy 72 16 , , , cord-266018-8bhnlsgy 72 17 CD86 CD86 NNP cord-266018-8bhnlsgy 72 18 , , , cord-266018-8bhnlsgy 72 19 and and CC cord-266018-8bhnlsgy 72 20 CD40 cd40 JJ cord-266018-8bhnlsgy 72 21 expression expression NN cord-266018-8bhnlsgy 72 22 was be VBD cord-266018-8bhnlsgy 72 23 determined determine VBN cord-266018-8bhnlsgy 72 24 by by IN cord-266018-8bhnlsgy 72 25 flow flow NN cord-266018-8bhnlsgy 72 26 cytometry cytometry NN cord-266018-8bhnlsgy 72 27 . . . cord-266018-8bhnlsgy 73 1 The the DT cord-266018-8bhnlsgy 73 2 mean mean JJ cord-266018-8bhnlsgy 73 3 fluorescence fluorescence NN cord-266018-8bhnlsgy 73 4 intensity intensity NN cord-266018-8bhnlsgy 73 5 ( ( -LRB- cord-266018-8bhnlsgy 73 6 MFI MFI NNP cord-266018-8bhnlsgy 73 7 ) ) -RRB- cord-266018-8bhnlsgy 73 8 for for IN cord-266018-8bhnlsgy 73 9 cells cell NNS cord-266018-8bhnlsgy 73 10 obtained obtain VBN cord-266018-8bhnlsgy 73 11 from from IN cord-266018-8bhnlsgy 73 12 either either CC cord-266018-8bhnlsgy 73 13 CCL3 CCL3 NNP cord-266018-8bhnlsgy 73 14 + + CD cord-266018-8bhnlsgy 74 1 /+ /+ NFP cord-266018-8bhnlsgy 74 2 or or CC cord-266018-8bhnlsgy 75 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 75 2 À à NN cord-266018-8bhnlsgy 75 3 / / SYM cord-266018-8bhnlsgy 75 4 À à NN cord-266018-8bhnlsgy 75 5 mice mouse NNS cord-266018-8bhnlsgy 75 6 is be VBZ cord-266018-8bhnlsgy 75 7 indicated indicate VBN cord-266018-8bhnlsgy 75 8 . . . cord-266018-8bhnlsgy 76 1 Flow flow NN cord-266018-8bhnlsgy 76 2 data datum NNS cord-266018-8bhnlsgy 76 3 shown show VBN cord-266018-8bhnlsgy 76 4 in in IN cord-266018-8bhnlsgy 76 5 panels panel NNS cord-266018-8bhnlsgy 76 6 A A NNP cord-266018-8bhnlsgy 76 7 , , , cord-266018-8bhnlsgy 76 8 B b NN cord-266018-8bhnlsgy 76 9 , , , cord-266018-8bhnlsgy 76 10 and and CC cord-266018-8bhnlsgy 76 11 D D NNP cord-266018-8bhnlsgy 76 12 are be VBP cord-266018-8bhnlsgy 76 13 representative representative JJ cord-266018-8bhnlsgy 76 14 of of IN cord-266018-8bhnlsgy 76 15 two two CD cord-266018-8bhnlsgy 76 16 separate separate JJ cord-266018-8bhnlsgy 76 17 experiments experiment NNS cord-266018-8bhnlsgy 76 18 with with IN cord-266018-8bhnlsgy 76 19 a a DT cord-266018-8bhnlsgy 76 20 total total NN cord-266018-8bhnlsgy 76 21 of of IN cord-266018-8bhnlsgy 76 22 10 10 CD cord-266018-8bhnlsgy 76 23 mice mouse NNS cord-266018-8bhnlsgy 76 24 for for IN cord-266018-8bhnlsgy 76 25 each each DT cord-266018-8bhnlsgy 76 26 experimental experimental JJ cord-266018-8bhnlsgy 76 27 condition condition NN cord-266018-8bhnlsgy 76 28 . . . cord-266018-8bhnlsgy 77 1 remaining remain VBG cord-266018-8bhnlsgy 77 2 CD11c CD11c NNP cord-266018-8bhnlsgy 77 3 + + CC cord-266018-8bhnlsgy 78 1 CD11b CD11b NNPS cord-266018-8bhnlsgy 78 2 À À NNP cord-266018-8bhnlsgy 78 3 CD8a cd8a NN cord-266018-8bhnlsgy 78 4 À à NN cord-266018-8bhnlsgy 78 5 population population NN cord-266018-8bhnlsgy 78 6 was be VBD cord-266018-8bhnlsgy 78 7 not not RB cord-266018-8bhnlsgy 78 8 further further RB cord-266018-8bhnlsgy 78 9 characterized characterize VBN cord-266018-8bhnlsgy 78 10 , , , cord-266018-8bhnlsgy 78 11 it -PRON- PRP cord-266018-8bhnlsgy 78 12 is be VBZ cord-266018-8bhnlsgy 78 13 likely likely JJ cord-266018-8bhnlsgy 78 14 that that IN cord-266018-8bhnlsgy 78 15 these these DT cord-266018-8bhnlsgy 78 16 cells cell NNS cord-266018-8bhnlsgy 78 17 may may MD cord-266018-8bhnlsgy 78 18 be be VB cord-266018-8bhnlsgy 78 19 plasmacytoid plasmacytoid NNP cord-266018-8bhnlsgy 78 20 in in IN cord-266018-8bhnlsgy 78 21 origin origin NN cord-266018-8bhnlsgy 79 1 ( ( -LRB- cord-266018-8bhnlsgy 79 2 CD11c CD11c NNP cord-266018-8bhnlsgy 79 3 + + SYM cord-266018-8bhnlsgy 79 4 CD11b CD11b NNS cord-266018-8bhnlsgy 79 5 À à NN cord-266018-8bhnlsgy 79 6 CD8a cd8a NN cord-266018-8bhnlsgy 79 7 À à NN cord-266018-8bhnlsgy 79 8 B220 B220 NNP cord-266018-8bhnlsgy 80 1 + + LS cord-266018-8bhnlsgy 80 2 ) ) -RRB- cord-266018-8bhnlsgy 80 3 or or CC cord-266018-8bhnlsgy 80 4 consist consist VBP cord-266018-8bhnlsgy 80 5 of of IN cord-266018-8bhnlsgy 80 6 a a DT cord-266018-8bhnlsgy 80 7 yet yet RB cord-266018-8bhnlsgy 80 8 to to TO cord-266018-8bhnlsgy 80 9 be be VB cord-266018-8bhnlsgy 80 10 defined define VBN cord-266018-8bhnlsgy 80 11 population population NN cord-266018-8bhnlsgy 80 12 of of IN cord-266018-8bhnlsgy 80 13 DC DC NNP cord-266018-8bhnlsgy 80 14 . . . cord-266018-8bhnlsgy 81 1 Comparison comparison NN cord-266018-8bhnlsgy 81 2 of of IN cord-266018-8bhnlsgy 81 3 the the DT cord-266018-8bhnlsgy 81 4 total total JJ cord-266018-8bhnlsgy 81 5 numbers number NNS cord-266018-8bhnlsgy 81 6 of of IN cord-266018-8bhnlsgy 81 7 CD8a CD8a NNP cord-266018-8bhnlsgy 81 8 + + CC cord-266018-8bhnlsgy 81 9 cells cell NNS cord-266018-8bhnlsgy 81 10 within within IN cord-266018-8bhnlsgy 81 11 the the DT cord-266018-8bhnlsgy 81 12 brains brain NNS cord-266018-8bhnlsgy 81 13 of of IN cord-266018-8bhnlsgy 81 14 MHV MHV NNP cord-266018-8bhnlsgy 81 15 - - HYPH cord-266018-8bhnlsgy 81 16 infected infect VBN cord-266018-8bhnlsgy 81 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 81 18 + + CC cord-266018-8bhnlsgy 81 19 /+ /+ . cord-266018-8bhnlsgy 81 20 and and CC cord-266018-8bhnlsgy 81 21 CCL3 CCL3 NNP cord-266018-8bhnlsgy 81 22 À À NNP cord-266018-8bhnlsgy 81 23 / / SYM cord-266018-8bhnlsgy 81 24 À à NN cord-266018-8bhnlsgy 81 25 mice mouse NNS cord-266018-8bhnlsgy 81 26 revealed reveal VBD cord-266018-8bhnlsgy 81 27 no no DT cord-266018-8bhnlsgy 81 28 dramatic dramatic JJ cord-266018-8bhnlsgy 81 29 differences difference NNS cord-266018-8bhnlsgy 81 30 between between IN cord-266018-8bhnlsgy 81 31 the the DT cord-266018-8bhnlsgy 81 32 two two CD cord-266018-8bhnlsgy 81 33 populations population NNS cord-266018-8bhnlsgy 81 34 of of IN cord-266018-8bhnlsgy 81 35 mice mouse NNS cord-266018-8bhnlsgy 81 36 at at IN cord-266018-8bhnlsgy 81 37 either either DT cord-266018-8bhnlsgy 81 38 2 2 CD cord-266018-8bhnlsgy 81 39 or or CC cord-266018-8bhnlsgy 81 40 5 5 CD cord-266018-8bhnlsgy 81 41 days day NNS cord-266018-8bhnlsgy 81 42 p.i p.i NNP cord-266018-8bhnlsgy 81 43 . . . cord-266018-8bhnlsgy 82 1 ( ( -LRB- cord-266018-8bhnlsgy 82 2 Fig Fig NNP cord-266018-8bhnlsgy 82 3 . . . cord-266018-8bhnlsgy 82 4 1C 1C NNP cord-266018-8bhnlsgy 82 5 ) ) -RRB- cord-266018-8bhnlsgy 82 6 . . . cord-266018-8bhnlsgy 83 1 In in IN cord-266018-8bhnlsgy 83 2 contrast contrast NN cord-266018-8bhnlsgy 83 3 , , , cord-266018-8bhnlsgy 83 4 numbers number NNS cord-266018-8bhnlsgy 83 5 of of IN cord-266018-8bhnlsgy 83 6 CD8a CD8a NNP cord-266018-8bhnlsgy 83 7 À à NN cord-266018-8bhnlsgy 83 8 cells cell NNS cord-266018-8bhnlsgy 83 9 were be VBD cord-266018-8bhnlsgy 83 10 increased increase VBN cord-266018-8bhnlsgy 83 11 by by IN cord-266018-8bhnlsgy 83 12 approximately approximately RB cord-266018-8bhnlsgy 83 13 30 30 CD cord-266018-8bhnlsgy 83 14 % % NN cord-266018-8bhnlsgy 83 15 within within IN cord-266018-8bhnlsgy 83 16 the the DT cord-266018-8bhnlsgy 83 17 brains brain NNS cord-266018-8bhnlsgy 83 18 of of IN cord-266018-8bhnlsgy 83 19 MHV MHV NNP cord-266018-8bhnlsgy 83 20 - - HYPH cord-266018-8bhnlsgy 83 21 infected infect VBN cord-266018-8bhnlsgy 83 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 83 23 + + CC cord-266018-8bhnlsgy 84 1 /+ /+ NFP cord-266018-8bhnlsgy 84 2 mice mouse NNS cord-266018-8bhnlsgy 84 3 as as IN cord-266018-8bhnlsgy 84 4 compared compare VBN cord-266018-8bhnlsgy 84 5 to to IN cord-266018-8bhnlsgy 84 6 CCL3 CCL3 NNP cord-266018-8bhnlsgy 84 7 À à NN cord-266018-8bhnlsgy 84 8 / / SYM cord-266018-8bhnlsgy 84 9 À à NN cord-266018-8bhnlsgy 84 10 mice mouse NNS cord-266018-8bhnlsgy 84 11 at at IN cord-266018-8bhnlsgy 84 12 day day NN cord-266018-8bhnlsgy 84 13 2 2 CD cord-266018-8bhnlsgy 84 14 p.i p.i NNP cord-266018-8bhnlsgy 84 15 . . . cord-266018-8bhnlsgy 85 1 However however RB cord-266018-8bhnlsgy 85 2 , , , cord-266018-8bhnlsgy 85 3 by by IN cord-266018-8bhnlsgy 85 4 day day NN cord-266018-8bhnlsgy 85 5 5 5 CD cord-266018-8bhnlsgy 85 6 p.i p.i NNP cord-266018-8bhnlsgy 85 7 . . NNP cord-266018-8bhnlsgy 85 8 , , , cord-266018-8bhnlsgy 85 9 there there EX cord-266018-8bhnlsgy 85 10 were be VBD cord-266018-8bhnlsgy 85 11 increased increase VBN cord-266018-8bhnlsgy 85 12 numbers number NNS cord-266018-8bhnlsgy 85 13 of of IN cord-266018-8bhnlsgy 85 14 CD8a CD8a NNP cord-266018-8bhnlsgy 85 15 À à NN cord-266018-8bhnlsgy 85 16 cells cell NNS cord-266018-8bhnlsgy 85 17 present present JJ cord-266018-8bhnlsgy 85 18 in in IN cord-266018-8bhnlsgy 85 19 the the DT cord-266018-8bhnlsgy 85 20 brains brain NNS cord-266018-8bhnlsgy 85 21 of of IN cord-266018-8bhnlsgy 85 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 85 23 À à NN cord-266018-8bhnlsgy 85 24 / / SYM cord-266018-8bhnlsgy 85 25 À à NN cord-266018-8bhnlsgy 85 26 mice mouse NNS cord-266018-8bhnlsgy 85 27 when when WRB cord-266018-8bhnlsgy 85 28 compared compare VBN cord-266018-8bhnlsgy 85 29 to to IN cord-266018-8bhnlsgy 85 30 CCL3 CCL3 NNP cord-266018-8bhnlsgy 85 31 + + CC cord-266018-8bhnlsgy 85 32 /+ /+ . cord-266018-8bhnlsgy 85 33 mice mouse NNS cord-266018-8bhnlsgy 85 34 . . . cord-266018-8bhnlsgy 86 1 In in IN cord-266018-8bhnlsgy 86 2 attempt attempt NN cord-266018-8bhnlsgy 86 3 to to TO cord-266018-8bhnlsgy 86 4 better better RB cord-266018-8bhnlsgy 86 5 evaluate evaluate VB cord-266018-8bhnlsgy 86 6 the the DT cord-266018-8bhnlsgy 86 7 activation activation NN cord-266018-8bhnlsgy 86 8 state state NN cord-266018-8bhnlsgy 86 9 of of IN cord-266018-8bhnlsgy 86 10 CD11c CD11c NNP cord-266018-8bhnlsgy 86 11 + + CC cord-266018-8bhnlsgy 86 12 cells cell NNS cord-266018-8bhnlsgy 86 13 within within IN cord-266018-8bhnlsgy 86 14 the the DT cord-266018-8bhnlsgy 86 15 brains brain NNS cord-266018-8bhnlsgy 86 16 of of IN cord-266018-8bhnlsgy 86 17 MHV mhv NN cord-266018-8bhnlsgy 86 18 - - HYPH cord-266018-8bhnlsgy 86 19 infected infect VBN cord-266018-8bhnlsgy 86 20 mice mouse NNS cord-266018-8bhnlsgy 86 21 , , , cord-266018-8bhnlsgy 86 22 we -PRON- PRP cord-266018-8bhnlsgy 86 23 next next RB cord-266018-8bhnlsgy 86 24 determined determine VBD cord-266018-8bhnlsgy 86 25 the the DT cord-266018-8bhnlsgy 86 26 expression expression NN cord-266018-8bhnlsgy 86 27 levels level NNS cord-266018-8bhnlsgy 86 28 of of IN cord-266018-8bhnlsgy 86 29 co co JJ cord-266018-8bhnlsgy 86 30 - - JJ cord-266018-8bhnlsgy 86 31 stimulatory stimulatory JJ cord-266018-8bhnlsgy 86 32 molecules molecule NNS cord-266018-8bhnlsgy 86 33 CD80 CD80 NNP cord-266018-8bhnlsgy 86 34 ( ( -LRB- cord-266018-8bhnlsgy 86 35 B-7.1 B-7.1 NNP cord-266018-8bhnlsgy 86 36 ) ) -RRB- cord-266018-8bhnlsgy 86 37 , , , cord-266018-8bhnlsgy 86 38 CD86 CD86 NNP cord-266018-8bhnlsgy 86 39 ( ( -LRB- cord-266018-8bhnlsgy 86 40 B-7.2 B-7.2 NNP cord-266018-8bhnlsgy 86 41 ) ) -RRB- cord-266018-8bhnlsgy 86 42 , , , cord-266018-8bhnlsgy 86 43 and and CC cord-266018-8bhnlsgy 86 44 CD40 CD40 NNP cord-266018-8bhnlsgy 86 45 on on IN cord-266018-8bhnlsgy 86 46 CD8a CD8a NNP cord-266018-8bhnlsgy 86 47 À à NN cord-266018-8bhnlsgy 86 48 cells cell NNS cord-266018-8bhnlsgy 86 49 within within IN cord-266018-8bhnlsgy 86 50 the the DT cord-266018-8bhnlsgy 86 51 CNS CNS NNP cord-266018-8bhnlsgy 86 52 of of IN cord-266018-8bhnlsgy 86 53 CCL3 CCL3 NNP cord-266018-8bhnlsgy 86 54 + + CC cord-266018-8bhnlsgy 86 55 /+ /+ . cord-266018-8bhnlsgy 86 56 and and CC cord-266018-8bhnlsgy 86 57 CCL3 CCL3 NNP cord-266018-8bhnlsgy 86 58 À À NNP cord-266018-8bhnlsgy 86 59 / / SYM cord-266018-8bhnlsgy 86 60 À à NN cord-266018-8bhnlsgy 86 61 mice mouse NNS cord-266018-8bhnlsgy 86 62 . . . cord-266018-8bhnlsgy 87 1 We -PRON- PRP cord-266018-8bhnlsgy 87 2 chose choose VBD cord-266018-8bhnlsgy 87 3 to to TO cord-266018-8bhnlsgy 87 4 focus focus VB cord-266018-8bhnlsgy 87 5 on on IN cord-266018-8bhnlsgy 87 6 this this DT cord-266018-8bhnlsgy 87 7 subpopulation subpopulation NN cord-266018-8bhnlsgy 87 8 of of IN cord-266018-8bhnlsgy 87 9 DCs dc NNS cord-266018-8bhnlsgy 87 10 in in IN cord-266018-8bhnlsgy 87 11 more more JJR cord-266018-8bhnlsgy 87 12 detail detail NN cord-266018-8bhnlsgy 87 13 as as IN cord-266018-8bhnlsgy 87 14 this this DT cord-266018-8bhnlsgy 87 15 clearly clearly RB cord-266018-8bhnlsgy 87 16 was be VBD cord-266018-8bhnlsgy 87 17 the the DT cord-266018-8bhnlsgy 87 18 predominant predominant JJ cord-266018-8bhnlsgy 87 19 DC DC NNP cord-266018-8bhnlsgy 87 20 population population NN cord-266018-8bhnlsgy 87 21 within within IN cord-266018-8bhnlsgy 87 22 the the DT cord-266018-8bhnlsgy 87 23 brains brain NNS cord-266018-8bhnlsgy 87 24 of of IN cord-266018-8bhnlsgy 87 25 infected infected JJ cord-266018-8bhnlsgy 87 26 mice mouse NNS cord-266018-8bhnlsgy 87 27 suggesting suggest VBG cord-266018-8bhnlsgy 87 28 a a DT cord-266018-8bhnlsgy 87 29 potentially potentially RB cord-266018-8bhnlsgy 87 30 more more RBR cord-266018-8bhnlsgy 87 31 important important JJ cord-266018-8bhnlsgy 87 32 role role NN cord-266018-8bhnlsgy 87 33 in in IN cord-266018-8bhnlsgy 87 34 defense defense NN cord-266018-8bhnlsgy 87 35 . . . cord-266018-8bhnlsgy 88 1 Analysis analysis NN cord-266018-8bhnlsgy 88 2 of of IN cord-266018-8bhnlsgy 88 3 CD8a CD8a NNP cord-266018-8bhnlsgy 88 4 À à NN cord-266018-8bhnlsgy 88 5 cells cell NNS cord-266018-8bhnlsgy 88 6 isolated isolate VBN cord-266018-8bhnlsgy 88 7 from from IN cord-266018-8bhnlsgy 88 8 the the DT cord-266018-8bhnlsgy 88 9 brains brain NNS cord-266018-8bhnlsgy 88 10 of of IN cord-266018-8bhnlsgy 88 11 CCL3 CCL3 NNP cord-266018-8bhnlsgy 88 12 + + CC cord-266018-8bhnlsgy 88 13 /+ /+ . cord-266018-8bhnlsgy 89 1 mice mouse NNS cord-266018-8bhnlsgy 89 2 at at IN cord-266018-8bhnlsgy 89 3 day day NN cord-266018-8bhnlsgy 89 4 2 2 CD cord-266018-8bhnlsgy 89 5 p.i p.i NNP cord-266018-8bhnlsgy 89 6 . . NNP cord-266018-8bhnlsgy 89 7 revealed reveal VBD cord-266018-8bhnlsgy 89 8 these these DT cord-266018-8bhnlsgy 89 9 cells cell NNS cord-266018-8bhnlsgy 89 10 expressed express VBD cord-266018-8bhnlsgy 89 11 detectable detectable JJ cord-266018-8bhnlsgy 89 12 levels level NNS cord-266018-8bhnlsgy 89 13 CD80 CD80 NNP cord-266018-8bhnlsgy 89 14 , , , cord-266018-8bhnlsgy 89 15 CD86 CD86 NNP cord-266018-8bhnlsgy 89 16 , , , cord-266018-8bhnlsgy 89 17 and and CC cord-266018-8bhnlsgy 89 18 CD40 CD40 NNP cord-266018-8bhnlsgy 89 19 as as IN cord-266018-8bhnlsgy 89 20 determined determine VBN cord-266018-8bhnlsgy 89 21 by by IN cord-266018-8bhnlsgy 89 22 measuring measure VBG cord-266018-8bhnlsgy 89 23 the the DT cord-266018-8bhnlsgy 89 24 mean mean JJ cord-266018-8bhnlsgy 89 25 fluorescence fluorescence NN cord-266018-8bhnlsgy 89 26 intensity intensity NN cord-266018-8bhnlsgy 89 27 ( ( -LRB- cord-266018-8bhnlsgy 89 28 MFI MFI NNP cord-266018-8bhnlsgy 89 29 ) ) -RRB- cord-266018-8bhnlsgy 89 30 ( ( -LRB- cord-266018-8bhnlsgy 89 31 Fig Fig NNP cord-266018-8bhnlsgy 89 32 . . . cord-266018-8bhnlsgy 89 33 1D 1d CD cord-266018-8bhnlsgy 89 34 ) ) -RRB- cord-266018-8bhnlsgy 89 35 . . . cord-266018-8bhnlsgy 90 1 Although although IN cord-266018-8bhnlsgy 90 2 MHV MHV NNP cord-266018-8bhnlsgy 90 3 infection infection NN cord-266018-8bhnlsgy 90 4 of of IN cord-266018-8bhnlsgy 90 5 CCL3 CCL3 NNP cord-266018-8bhnlsgy 90 6 À à NN cord-266018-8bhnlsgy 90 7 / / SYM cord-266018-8bhnlsgy 90 8 À à NN cord-266018-8bhnlsgy 90 9 mice mouse NNS cord-266018-8bhnlsgy 90 10 also also RB cord-266018-8bhnlsgy 90 11 resulted result VBD cord-266018-8bhnlsgy 90 12 in in IN cord-266018-8bhnlsgy 90 13 enhanced enhanced JJ cord-266018-8bhnlsgy 90 14 expression expression NN cord-266018-8bhnlsgy 90 15 of of IN cord-266018-8bhnlsgy 90 16 CD86 CD86 NNP cord-266018-8bhnlsgy 90 17 on on IN cord-266018-8bhnlsgy 90 18 the the DT cord-266018-8bhnlsgy 90 19 surface surface NN cord-266018-8bhnlsgy 90 20 of of IN cord-266018-8bhnlsgy 90 21 CD8a CD8a NNP cord-266018-8bhnlsgy 90 22 À à NN cord-266018-8bhnlsgy 90 23 cells cell NNS cord-266018-8bhnlsgy 90 24 , , , cord-266018-8bhnlsgy 90 25 the the DT cord-266018-8bhnlsgy 90 26 MFI MFI NNP cord-266018-8bhnlsgy 90 27 for for IN cord-266018-8bhnlsgy 90 28 both both DT cord-266018-8bhnlsgy 90 29 CD80 CD80 NNP cord-266018-8bhnlsgy 90 30 and and CC cord-266018-8bhnlsgy 90 31 CD40 CD40 NNP cord-266018-8bhnlsgy 90 32 was be VBD cord-266018-8bhnlsgy 90 33 dramatically dramatically RB cord-266018-8bhnlsgy 90 34 reduced reduce VBN cord-266018-8bhnlsgy 90 35 as as IN cord-266018-8bhnlsgy 90 36 compared compare VBN cord-266018-8bhnlsgy 90 37 to to IN cord-266018-8bhnlsgy 90 38 CD8a CD8a NNP cord-266018-8bhnlsgy 90 39 À à NN cord-266018-8bhnlsgy 90 40 cells cell NNS cord-266018-8bhnlsgy 90 41 within within IN cord-266018-8bhnlsgy 90 42 the the DT cord-266018-8bhnlsgy 90 43 brain brain NN cord-266018-8bhnlsgy 90 44 of of IN cord-266018-8bhnlsgy 90 45 CCL3 CCL3 NNP cord-266018-8bhnlsgy 90 46 + + CC cord-266018-8bhnlsgy 90 47 /+ /+ . cord-266018-8bhnlsgy 91 1 mice mouse NNS cord-266018-8bhnlsgy 91 2 at at IN cord-266018-8bhnlsgy 91 3 day day NN cord-266018-8bhnlsgy 91 4 2 2 CD cord-266018-8bhnlsgy 91 5 p.i p.i NNP cord-266018-8bhnlsgy 91 6 . . . cord-266018-8bhnlsgy 92 1 ( ( -LRB- cord-266018-8bhnlsgy 92 2 Fig Fig NNP cord-266018-8bhnlsgy 92 3 . . . cord-266018-8bhnlsgy 92 4 1D 1d CD cord-266018-8bhnlsgy 92 5 ) ) -RRB- cord-266018-8bhnlsgy 92 6 . . . cord-266018-8bhnlsgy 93 1 Together together RB cord-266018-8bhnlsgy 93 2 , , , cord-266018-8bhnlsgy 93 3 these these DT cord-266018-8bhnlsgy 93 4 results result NNS cord-266018-8bhnlsgy 93 5 indicate indicate VBP cord-266018-8bhnlsgy 93 6 that that IN cord-266018-8bhnlsgy 93 7 although although IN cord-266018-8bhnlsgy 93 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 93 9 signaling signaling NN cord-266018-8bhnlsgy 93 10 is be VBZ cord-266018-8bhnlsgy 93 11 not not RB cord-266018-8bhnlsgy 93 12 required require VBN cord-266018-8bhnlsgy 93 13 for for IN cord-266018-8bhnlsgy 93 14 the the DT cord-266018-8bhnlsgy 93 15 appearance appearance NN cord-266018-8bhnlsgy 93 16 of of IN cord-266018-8bhnlsgy 93 17 DC DC NNP cord-266018-8bhnlsgy 93 18 - - HYPH cord-266018-8bhnlsgy 93 19 like like JJ cord-266018-8bhnlsgy 93 20 cells cell NNS cord-266018-8bhnlsgy 93 21 within within IN cord-266018-8bhnlsgy 93 22 the the DT cord-266018-8bhnlsgy 93 23 brain brain NN cord-266018-8bhnlsgy 93 24 , , , cord-266018-8bhnlsgy 93 25 expression expression NN cord-266018-8bhnlsgy 93 26 of of IN cord-266018-8bhnlsgy 93 27 the the DT cord-266018-8bhnlsgy 93 28 co co JJ cord-266018-8bhnlsgy 93 29 - - JJ cord-266018-8bhnlsgy 93 30 stimulatory stimulatory JJ cord-266018-8bhnlsgy 93 31 molecules molecule NNS cord-266018-8bhnlsgy 93 32 CD40 CD40 NNP cord-266018-8bhnlsgy 93 33 and and CC cord-266018-8bhnlsgy 93 34 CD80 CD80 NNP cord-266018-8bhnlsgy 93 35 is be VBZ cord-266018-8bhnlsgy 93 36 muted mute VBN cord-266018-8bhnlsgy 93 37 in in IN cord-266018-8bhnlsgy 93 38 the the DT cord-266018-8bhnlsgy 93 39 absence absence NN cord-266018-8bhnlsgy 93 40 of of IN cord-266018-8bhnlsgy 93 41 CCL3 CCL3 NNP cord-266018-8bhnlsgy 93 42 signaling signaling NN cord-266018-8bhnlsgy 93 43 . . . cord-266018-8bhnlsgy 94 1 Characterization characterization NN cord-266018-8bhnlsgy 94 2 of of IN cord-266018-8bhnlsgy 94 3 CD11c CD11c NNP cord-266018-8bhnlsgy 94 4 + + CC cord-266018-8bhnlsgy 94 5 cells cell NNS cord-266018-8bhnlsgy 94 6 within within IN cord-266018-8bhnlsgy 94 7 the the DT cord-266018-8bhnlsgy 94 8 CLN cln NN cord-266018-8bhnlsgy 94 9 of of IN cord-266018-8bhnlsgy 94 10 MHV MHV NNP cord-266018-8bhnlsgy 94 11 - - HYPH cord-266018-8bhnlsgy 94 12 infected infect VBN cord-266018-8bhnlsgy 94 13 CCL3 CCL3 NNP cord-266018-8bhnlsgy 94 14 + + CC cord-266018-8bhnlsgy 94 15 /+ /+ . cord-266018-8bhnlsgy 94 16 and and CC cord-266018-8bhnlsgy 94 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 94 18 À À NNP cord-266018-8bhnlsgy 94 19 / / SYM cord-266018-8bhnlsgy 94 20 À à NN cord-266018-8bhnlsgy 94 21 mice mouse NNS cord-266018-8bhnlsgy 95 1 Following follow VBG cord-266018-8bhnlsgy 95 2 MHV MHV NNP cord-266018-8bhnlsgy 95 3 infection infection NN cord-266018-8bhnlsgy 95 4 of of IN cord-266018-8bhnlsgy 95 5 the the DT cord-266018-8bhnlsgy 95 6 CNS CNS NNP cord-266018-8bhnlsgy 95 7 , , , cord-266018-8bhnlsgy 95 8 virus virus NN cord-266018-8bhnlsgy 95 9 - - HYPH cord-266018-8bhnlsgy 95 10 specific specific JJ cord-266018-8bhnlsgy 95 11 T t NN cord-266018-8bhnlsgy 95 12 cells cell NNS cord-266018-8bhnlsgy 95 13 are be VBP cord-266018-8bhnlsgy 95 14 present present JJ cord-266018-8bhnlsgy 95 15 within within IN cord-266018-8bhnlsgy 95 16 the the DT cord-266018-8bhnlsgy 95 17 CLN cln NN cord-266018-8bhnlsgy 95 18 suggesting suggest VBG cord-266018-8bhnlsgy 95 19 that that IN cord-266018-8bhnlsgy 95 20 the the DT cord-266018-8bhnlsgy 95 21 bulk bulk NN cord-266018-8bhnlsgy 95 22 of of IN cord-266018-8bhnlsgy 95 23 virus virus NN cord-266018-8bhnlsgy 95 24 - - HYPH cord-266018-8bhnlsgy 95 25 specific specific JJ cord-266018-8bhnlsgy 95 26 T t NN cord-266018-8bhnlsgy 95 27 cells cell NNS cord-266018-8bhnlsgy 95 28 are be VBP cord-266018-8bhnlsgy 95 29 generated generate VBN cord-266018-8bhnlsgy 95 30 in in IN cord-266018-8bhnlsgy 95 31 the the DT cord-266018-8bhnlsgy 95 32 periphery periphery NN cord-266018-8bhnlsgy 96 1 ( ( -LRB- cord-266018-8bhnlsgy 96 2 Marten Marten NNP cord-266018-8bhnlsgy 96 3 et et NNP cord-266018-8bhnlsgy 96 4 al al NNP cord-266018-8bhnlsgy 96 5 . . NNP cord-266018-8bhnlsgy 96 6 , , , cord-266018-8bhnlsgy 96 7 2003 2003 CD cord-266018-8bhnlsgy 96 8 ) ) -RRB- cord-266018-8bhnlsgy 96 9 . . . cord-266018-8bhnlsgy 97 1 Therefore therefore RB cord-266018-8bhnlsgy 97 2 , , , cord-266018-8bhnlsgy 97 3 the the DT cord-266018-8bhnlsgy 97 4 accumulation accumulation NN cord-266018-8bhnlsgy 97 5 of of IN cord-266018-8bhnlsgy 97 6 CD11c CD11c NNP cord-266018-8bhnlsgy 97 7 + + CC cord-266018-8bhnlsgy 97 8 cells cell NNS cord-266018-8bhnlsgy 97 9 within within IN cord-266018-8bhnlsgy 97 10 the the DT cord-266018-8bhnlsgy 97 11 CLN cln NN cord-266018-8bhnlsgy 97 12 of of IN cord-266018-8bhnlsgy 97 13 infected infect VBN cord-266018-8bhnlsgy 97 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 97 15 + + CC cord-266018-8bhnlsgy 97 16 /+ /+ . cord-266018-8bhnlsgy 97 17 and and CC cord-266018-8bhnlsgy 97 18 CCL3 CCL3 NNP cord-266018-8bhnlsgy 97 19 À À NNP cord-266018-8bhnlsgy 97 20 / / SYM cord-266018-8bhnlsgy 97 21 À à NN cord-266018-8bhnlsgy 97 22 mice mouse NNS cord-266018-8bhnlsgy 97 23 was be VBD cord-266018-8bhnlsgy 97 24 determined determine VBN cord-266018-8bhnlsgy 97 25 . . . cord-266018-8bhnlsgy 98 1 Before before IN cord-266018-8bhnlsgy 98 2 infection infection NN cord-266018-8bhnlsgy 98 3 , , , cord-266018-8bhnlsgy 98 4 CD11c CD11c NNP cord-266018-8bhnlsgy 98 5 + + CC cord-266018-8bhnlsgy 98 6 cells cell NNS cord-266018-8bhnlsgy 98 7 expressing express VBG cord-266018-8bhnlsgy 98 8 a a DT cord-266018-8bhnlsgy 98 9 myeloid myeloid JJ cord-266018-8bhnlsgy 98 10 DC DC NNP cord-266018-8bhnlsgy 98 11 phenotype phenotype NN cord-266018-8bhnlsgy 98 12 ( ( -LRB- cord-266018-8bhnlsgy 98 13 CD11c CD11c NNP cord-266018-8bhnlsgy 98 14 + + SYM cord-266018-8bhnlsgy 98 15 CD11b CD11b NNS cord-266018-8bhnlsgy 98 16 + + -RRB- cord-266018-8bhnlsgy 98 17 ) ) -RRB- cord-266018-8bhnlsgy 98 18 and and CC cord-266018-8bhnlsgy 98 19 lymphoid lymphoid JJ cord-266018-8bhnlsgy 98 20 DC DC NNP cord-266018-8bhnlsgy 98 21 phenotype phenotype NN cord-266018-8bhnlsgy 98 22 ( ( -LRB- cord-266018-8bhnlsgy 98 23 CD11c CD11c NNP cord-266018-8bhnlsgy 98 24 + + SYM cord-266018-8bhnlsgy 98 25 CD11b CD11b NNS cord-266018-8bhnlsgy 98 26 À à NN cord-266018-8bhnlsgy 98 27 ) ) -RRB- cord-266018-8bhnlsgy 98 28 were be VBD cord-266018-8bhnlsgy 98 29 present present JJ cord-266018-8bhnlsgy 98 30 within within IN cord-266018-8bhnlsgy 98 31 the the DT cord-266018-8bhnlsgy 98 32 CLN cln NN cord-266018-8bhnlsgy 98 33 of of IN cord-266018-8bhnlsgy 98 34 both both DT cord-266018-8bhnlsgy 98 35 CCL3 CCL3 NNP cord-266018-8bhnlsgy 98 36 + + CC cord-266018-8bhnlsgy 99 1 /+ /+ . cord-266018-8bhnlsgy 100 1 and and CC cord-266018-8bhnlsgy 100 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 100 3 À À NNP cord-266018-8bhnlsgy 100 4 / / SYM cord-266018-8bhnlsgy 100 5 À à NN cord-266018-8bhnlsgy 100 6 mice mouse NNS cord-266018-8bhnlsgy 100 7 at at IN cord-266018-8bhnlsgy 100 8 an an DT cord-266018-8bhnlsgy 100 9 approximate approximate JJ cord-266018-8bhnlsgy 100 10 1:2 1:2 CD cord-266018-8bhnlsgy 100 11 ratio ratio NN cord-266018-8bhnlsgy 100 12 , , , cord-266018-8bhnlsgy 100 13 respectively respectively RB cord-266018-8bhnlsgy 101 1 ( ( -LRB- cord-266018-8bhnlsgy 101 2 Fig Fig NNP cord-266018-8bhnlsgy 101 3 . . . cord-266018-8bhnlsgy 101 4 2A 2a CD cord-266018-8bhnlsgy 101 5 ) ) -RRB- cord-266018-8bhnlsgy 101 6 . . . cord-266018-8bhnlsgy 102 1 However however RB cord-266018-8bhnlsgy 102 2 , , , cord-266018-8bhnlsgy 102 3 within within IN cord-266018-8bhnlsgy 102 4 2 2 CD cord-266018-8bhnlsgy 102 5 days day NNS cord-266018-8bhnlsgy 102 6 of of IN cord-266018-8bhnlsgy 102 7 MHV MHV NNP cord-266018-8bhnlsgy 102 8 infection infection NN cord-266018-8bhnlsgy 102 9 of of IN cord-266018-8bhnlsgy 102 10 the the DT cord-266018-8bhnlsgy 102 11 CNS CNS NNP cord-266018-8bhnlsgy 102 12 , , , cord-266018-8bhnlsgy 102 13 the the DT cord-266018-8bhnlsgy 102 14 frequency frequency NN cord-266018-8bhnlsgy 102 15 of of IN cord-266018-8bhnlsgy 102 16 CD11c CD11c NNP cord-266018-8bhnlsgy 102 17 + + SYM cord-266018-8bhnlsgy 102 18 -CD11b -CD11b NNS cord-266018-8bhnlsgy 102 19 + + CC cord-266018-8bhnlsgy 102 20 DCs dc NNS cord-266018-8bhnlsgy 102 21 , , , cord-266018-8bhnlsgy 102 22 but but CC cord-266018-8bhnlsgy 102 23 not not RB cord-266018-8bhnlsgy 102 24 CD11c CD11c NNP cord-266018-8bhnlsgy 102 25 + + SYM cord-266018-8bhnlsgy 102 26 CD11b CD11b NNPS cord-266018-8bhnlsgy 102 27 À À NNP cord-266018-8bhnlsgy 102 28 DCs dc NNS cord-266018-8bhnlsgy 102 29 , , , cord-266018-8bhnlsgy 102 30 dramatically dramatically RB cord-266018-8bhnlsgy 102 31 increased increase VBD cord-266018-8bhnlsgy 102 32 within within IN cord-266018-8bhnlsgy 102 33 the the DT cord-266018-8bhnlsgy 102 34 CLN cln NN cord-266018-8bhnlsgy 102 35 of of IN cord-266018-8bhnlsgy 102 36 CCL3 CCL3 NNP cord-266018-8bhnlsgy 102 37 + + CC cord-266018-8bhnlsgy 102 38 /+ /+ . cord-266018-8bhnlsgy 103 1 mice mouse NNS cord-266018-8bhnlsgy 103 2 ( ( -LRB- cord-266018-8bhnlsgy 103 3 Fig Fig NNP cord-266018-8bhnlsgy 103 4 . . . cord-266018-8bhnlsgy 103 5 2A 2a CD cord-266018-8bhnlsgy 103 6 ) ) -RRB- cord-266018-8bhnlsgy 103 7 . . . cord-266018-8bhnlsgy 104 1 Further further JJ cord-266018-8bhnlsgy 104 2 analysis analysis NN cord-266018-8bhnlsgy 104 3 revealed reveal VBD cord-266018-8bhnlsgy 104 4 that that IN cord-266018-8bhnlsgy 104 5 the the DT cord-266018-8bhnlsgy 104 6 CD11c CD11c NNP cord-266018-8bhnlsgy 104 7 + + SYM cord-266018-8bhnlsgy 104 8 CD11b CD11b NNS cord-266018-8bhnlsgy 104 9 + + CC cord-266018-8bhnlsgy 104 10 cell cell NN cord-266018-8bhnlsgy 104 11 population population NN cord-266018-8bhnlsgy 104 12 was be VBD cord-266018-8bhnlsgy 104 13 CD8a CD8a NNP cord-266018-8bhnlsgy 104 14 À À NNP cord-266018-8bhnlsgy 104 15 DEC205 DEC205 NNP cord-266018-8bhnlsgy 104 16 À à NN cord-266018-8bhnlsgy 104 17 while while NN cord-266018-8bhnlsgy 105 1 the the DT cord-266018-8bhnlsgy 105 2 CD11c CD11c NNP cord-266018-8bhnlsgy 105 3 + + SYM cord-266018-8bhnlsgy 105 4 -CD11b -CD11b : cord-266018-8bhnlsgy 105 5 À à NN cord-266018-8bhnlsgy 105 6 population population NN cord-266018-8bhnlsgy 105 7 was be VBD cord-266018-8bhnlsgy 105 8 CD8a CD8a NNP cord-266018-8bhnlsgy 106 1 + + CC cord-266018-8bhnlsgy 107 1 DEC205 DEC205 NNP cord-266018-8bhnlsgy 107 2 + + CC cord-266018-8bhnlsgy 108 1 ( ( -LRB- cord-266018-8bhnlsgy 108 2 Fig Fig NNP cord-266018-8bhnlsgy 108 3 . . . cord-266018-8bhnlsgy 108 4 2B 2b CD cord-266018-8bhnlsgy 108 5 ) ) -RRB- cord-266018-8bhnlsgy 108 6 . . . cord-266018-8bhnlsgy 109 1 Although although IN cord-266018-8bhnlsgy 109 2 the the DT cord-266018-8bhnlsgy 109 3 total total JJ cord-266018-8bhnlsgy 109 4 number number NN cord-266018-8bhnlsgy 109 5 of of IN cord-266018-8bhnlsgy 109 6 CD8a CD8a NNP cord-266018-8bhnlsgy 109 7 À à NN cord-266018-8bhnlsgy 109 8 cells cell NNS cord-266018-8bhnlsgy 109 9 increased increase VBD cord-266018-8bhnlsgy 109 10 within within IN cord-266018-8bhnlsgy 109 11 the the DT cord-266018-8bhnlsgy 109 12 CLN cln NN cord-266018-8bhnlsgy 109 13 of of IN cord-266018-8bhnlsgy 109 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 109 15 À À NNP cord-266018-8bhnlsgy 109 16 / / SYM cord-266018-8bhnlsgy 109 17 À à NN cord-266018-8bhnlsgy 109 18 mice mouse NNS cord-266018-8bhnlsgy 109 19 , , , cord-266018-8bhnlsgy 109 20 there there EX cord-266018-8bhnlsgy 109 21 was be VBD cord-266018-8bhnlsgy 109 22 an an DT cord-266018-8bhnlsgy 109 23 approximate approximate JJ cord-266018-8bhnlsgy 109 24 4-fold 4-fold CD cord-266018-8bhnlsgy 109 25 reduction reduction NN cord-266018-8bhnlsgy 109 26 in in IN cord-266018-8bhnlsgy 109 27 total total JJ cord-266018-8bhnlsgy 109 28 numbers number NNS cord-266018-8bhnlsgy 109 29 of of IN cord-266018-8bhnlsgy 109 30 CD8a CD8a NNP cord-266018-8bhnlsgy 109 31 À à NN cord-266018-8bhnlsgy 109 32 cells cell NNS cord-266018-8bhnlsgy 109 33 as as IN cord-266018-8bhnlsgy 109 34 compared compare VBN cord-266018-8bhnlsgy 109 35 to to IN cord-266018-8bhnlsgy 109 36 CCL3 CCL3 NNP cord-266018-8bhnlsgy 109 37 + + CC cord-266018-8bhnlsgy 109 38 /+ /+ . cord-266018-8bhnlsgy 110 1 mice mouse NNS cord-266018-8bhnlsgy 110 2 at at IN cord-266018-8bhnlsgy 110 3 days day NNS cord-266018-8bhnlsgy 110 4 2 2 CD cord-266018-8bhnlsgy 110 5 and and CC cord-266018-8bhnlsgy 110 6 5 5 CD cord-266018-8bhnlsgy 110 7 p.i p.i NNP cord-266018-8bhnlsgy 110 8 . . . cord-266018-8bhnlsgy 110 9 ( ( -LRB- cord-266018-8bhnlsgy 110 10 Fig Fig NNP cord-266018-8bhnlsgy 110 11 . . . cord-266018-8bhnlsgy 110 12 2C 2C NNP cord-266018-8bhnlsgy 110 13 ) ) -RRB- cord-266018-8bhnlsgy 110 14 . . . cord-266018-8bhnlsgy 111 1 Similar similar JJ cord-266018-8bhnlsgy 111 2 to to IN cord-266018-8bhnlsgy 111 3 the the DT cord-266018-8bhnlsgy 111 4 brain brain NN cord-266018-8bhnlsgy 111 5 , , , cord-266018-8bhnlsgy 111 6 no no DT cord-266018-8bhnlsgy 111 7 difference difference NN cord-266018-8bhnlsgy 111 8 in in IN cord-266018-8bhnlsgy 111 9 numbers number NNS cord-266018-8bhnlsgy 111 10 of of IN cord-266018-8bhnlsgy 111 11 CD8a CD8a NNP cord-266018-8bhnlsgy 111 12 + + CC cord-266018-8bhnlsgy 112 1 cells cell NNS cord-266018-8bhnlsgy 112 2 within within IN cord-266018-8bhnlsgy 112 3 the the DT cord-266018-8bhnlsgy 112 4 CLN cln NN cord-266018-8bhnlsgy 112 5 was be VBD cord-266018-8bhnlsgy 112 6 detected detect VBN cord-266018-8bhnlsgy 112 7 at at IN cord-266018-8bhnlsgy 112 8 either either DT cord-266018-8bhnlsgy 112 9 days day NNS cord-266018-8bhnlsgy 112 10 2 2 CD cord-266018-8bhnlsgy 112 11 or or CC cord-266018-8bhnlsgy 112 12 5 5 CD cord-266018-8bhnlsgy 112 13 p.i p.i NNP cord-266018-8bhnlsgy 112 14 . . . cord-266018-8bhnlsgy 113 1 ( ( -LRB- cord-266018-8bhnlsgy 113 2 Fig Fig NNP cord-266018-8bhnlsgy 113 3 . . . cord-266018-8bhnlsgy 113 4 2C 2C NNP cord-266018-8bhnlsgy 113 5 ) ) -RRB- cord-266018-8bhnlsgy 113 6 . . . cord-266018-8bhnlsgy 114 1 Examination examination NN cord-266018-8bhnlsgy 114 2 of of IN cord-266018-8bhnlsgy 114 3 co co JJ cord-266018-8bhnlsgy 114 4 - - JJ cord-266018-8bhnlsgy 114 5 stimulatory stimulatory JJ cord-266018-8bhnlsgy 114 6 molecule molecule NN cord-266018-8bhnlsgy 114 7 expression expression NN cord-266018-8bhnlsgy 114 8 on on IN cord-266018-8bhnlsgy 114 9 CD8a CD8a NNP cord-266018-8bhnlsgy 114 10 À À HYPH cord-266018-8bhnlsgy 114 11 DCs dc NNS cord-266018-8bhnlsgy 114 12 present present NN cord-266018-8bhnlsgy 114 13 within within IN cord-266018-8bhnlsgy 114 14 the the DT cord-266018-8bhnlsgy 114 15 CLN cln NN cord-266018-8bhnlsgy 114 16 of of IN cord-266018-8bhnlsgy 114 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 114 18 + + CC cord-266018-8bhnlsgy 114 19 /+ /+ . cord-266018-8bhnlsgy 115 1 mice mouse NNS cord-266018-8bhnlsgy 115 2 correlated correlate VBD cord-266018-8bhnlsgy 115 3 with with IN cord-266018-8bhnlsgy 115 4 the the DT cord-266018-8bhnlsgy 115 5 increased increase VBN cord-266018-8bhnlsgy 115 6 expression expression NN cord-266018-8bhnlsgy 115 7 of of IN cord-266018-8bhnlsgy 115 8 co co JJ cord-266018-8bhnlsgy 115 9 - - JJ cord-266018-8bhnlsgy 115 10 stimulatory stimulatory JJ cord-266018-8bhnlsgy 115 11 molecules molecule NNS cord-266018-8bhnlsgy 115 12 CD40 CD40 NNP cord-266018-8bhnlsgy 115 13 , , , cord-266018-8bhnlsgy 115 14 CD80 CD80 NNP cord-266018-8bhnlsgy 115 15 , , , cord-266018-8bhnlsgy 115 16 CD86 CD86 NNP cord-266018-8bhnlsgy 115 17 as as RB cord-266018-8bhnlsgy 115 18 well well RB cord-266018-8bhnlsgy 115 19 as as IN cord-266018-8bhnlsgy 115 20 increased increase VBN cord-266018-8bhnlsgy 115 21 MHC MHC NNP cord-266018-8bhnlsgy 115 22 I I NNP cord-266018-8bhnlsgy 115 23 and and CC cord-266018-8bhnlsgy 115 24 II ii CD cord-266018-8bhnlsgy 115 25 expression expression NN cord-266018-8bhnlsgy 115 26 when when WRB cord-266018-8bhnlsgy 115 27 compared compare VBN cord-266018-8bhnlsgy 115 28 to to IN cord-266018-8bhnlsgy 115 29 sham sham NN cord-266018-8bhnlsgy 115 30 - - HYPH cord-266018-8bhnlsgy 115 31 infected infected JJ cord-266018-8bhnlsgy 115 32 mice mouse NNS cord-266018-8bhnlsgy 115 33 ( ( -LRB- cord-266018-8bhnlsgy 115 34 Figs fig NNS cord-266018-8bhnlsgy 115 35 . . . cord-266018-8bhnlsgy 116 1 2D 2D NNP cord-266018-8bhnlsgy 116 2 and and CC cord-266018-8bhnlsgy 116 3 E E NNP cord-266018-8bhnlsgy 116 4 ) ) -RRB- cord-266018-8bhnlsgy 116 5 . . . cord-266018-8bhnlsgy 117 1 These these DT cord-266018-8bhnlsgy 117 2 data datum NNS cord-266018-8bhnlsgy 117 3 suggest suggest VBP cord-266018-8bhnlsgy 117 4 that that IN cord-266018-8bhnlsgy 117 5 this this DT cord-266018-8bhnlsgy 117 6 population population NN cord-266018-8bhnlsgy 117 7 of of IN cord-266018-8bhnlsgy 117 8 activated activate VBN cord-266018-8bhnlsgy 117 9 CD8a CD8a NNP cord-266018-8bhnlsgy 117 10 À à NN cord-266018-8bhnlsgy 117 11 cells cell NNS cord-266018-8bhnlsgy 117 12 within within IN cord-266018-8bhnlsgy 117 13 the the DT cord-266018-8bhnlsgy 117 14 CLN cln NN cord-266018-8bhnlsgy 117 15 is be VBZ cord-266018-8bhnlsgy 117 16 able able JJ cord-266018-8bhnlsgy 117 17 to to TO cord-266018-8bhnlsgy 117 18 present present VB cord-266018-8bhnlsgy 117 19 antigen antigen NN cord-266018-8bhnlsgy 117 20 and and CC cord-266018-8bhnlsgy 117 21 induce induce VB cord-266018-8bhnlsgy 117 22 T T NNP cord-266018-8bhnlsgy 117 23 cell cell NN cord-266018-8bhnlsgy 117 24 differentiation differentiation NN cord-266018-8bhnlsgy 117 25 within within IN cord-266018-8bhnlsgy 117 26 the the DT cord-266018-8bhnlsgy 117 27 CLN cln NN cord-266018-8bhnlsgy 117 28 following follow VBG cord-266018-8bhnlsgy 117 29 MHV MHV NNP cord-266018-8bhnlsgy 117 30 infection infection NN cord-266018-8bhnlsgy 117 31 of of IN cord-266018-8bhnlsgy 117 32 the the DT cord-266018-8bhnlsgy 117 33 CNS CNS NNP cord-266018-8bhnlsgy 117 34 . . . cord-266018-8bhnlsgy 118 1 Although although IN cord-266018-8bhnlsgy 118 2 CD8a CD8a NNP cord-266018-8bhnlsgy 118 3 À À HYPH cord-266018-8bhnlsgy 118 4 DCs dc NNS cord-266018-8bhnlsgy 118 5 isolated isolate VBN cord-266018-8bhnlsgy 118 6 from from IN cord-266018-8bhnlsgy 118 7 the the DT cord-266018-8bhnlsgy 118 8 CLN cln NN cord-266018-8bhnlsgy 118 9 of of IN cord-266018-8bhnlsgy 118 10 CCL3 CCL3 NNP cord-266018-8bhnlsgy 118 11 À À NNP cord-266018-8bhnlsgy 118 12 / / SYM cord-266018-8bhnlsgy 118 13 À à NN cord-266018-8bhnlsgy 118 14 mice mouse NNS cord-266018-8bhnlsgy 118 15 expressed express VBD cord-266018-8bhnlsgy 118 16 similar similar JJ cord-266018-8bhnlsgy 118 17 levels level NNS cord-266018-8bhnlsgy 118 18 of of IN cord-266018-8bhnlsgy 118 19 CD80 CD80 NNP cord-266018-8bhnlsgy 118 20 and and CC cord-266018-8bhnlsgy 118 21 CD86 CD86 NNP cord-266018-8bhnlsgy 118 22 as as IN cord-266018-8bhnlsgy 118 23 compared compare VBN cord-266018-8bhnlsgy 118 24 to to IN cord-266018-8bhnlsgy 118 25 CCL3 CCL3 NNP cord-266018-8bhnlsgy 118 26 + + CC cord-266018-8bhnlsgy 118 27 /+ /+ . cord-266018-8bhnlsgy 119 1 ( ( -LRB- cord-266018-8bhnlsgy 119 2 determined determine VBN cord-266018-8bhnlsgy 119 3 by by IN cord-266018-8bhnlsgy 119 4 MFI MFI NNP cord-266018-8bhnlsgy 119 5 ) ) -RRB- cord-266018-8bhnlsgy 119 6 , , , cord-266018-8bhnlsgy 119 7 expression expression NN cord-266018-8bhnlsgy 119 8 of of IN cord-266018-8bhnlsgy 119 9 CD40 CD40 NNP cord-266018-8bhnlsgy 119 10 as as RB cord-266018-8bhnlsgy 119 11 well well RB cord-266018-8bhnlsgy 119 12 as as IN cord-266018-8bhnlsgy 119 13 MHC MHC NNP cord-266018-8bhnlsgy 119 14 I I NNP cord-266018-8bhnlsgy 119 15 and and CC cord-266018-8bhnlsgy 119 16 II II NNP cord-266018-8bhnlsgy 119 17 were be VBD cord-266018-8bhnlsgy 119 18 reduced reduce VBN cord-266018-8bhnlsgy 119 19 as as IN cord-266018-8bhnlsgy 119 20 compared compare VBN cord-266018-8bhnlsgy 119 21 to to IN cord-266018-8bhnlsgy 119 22 CD8a CD8a NNP cord-266018-8bhnlsgy 119 23 À à NN cord-266018-8bhnlsgy 119 24 cells cell NNS cord-266018-8bhnlsgy 119 25 isolated isolate VBN cord-266018-8bhnlsgy 119 26 from from IN cord-266018-8bhnlsgy 119 27 MHV MHV NNP cord-266018-8bhnlsgy 119 28 - - HYPH cord-266018-8bhnlsgy 119 29 infected infect VBN cord-266018-8bhnlsgy 119 30 CCL3 CCL3 NNP cord-266018-8bhnlsgy 119 31 + + CC cord-266018-8bhnlsgy 119 32 /+ /+ . cord-266018-8bhnlsgy 119 33 mice mouse NNS cord-266018-8bhnlsgy 119 34 ( ( -LRB- cord-266018-8bhnlsgy 119 35 Figs fig NNS cord-266018-8bhnlsgy 119 36 . . . cord-266018-8bhnlsgy 120 1 2D 2D NNP cord-266018-8bhnlsgy 120 2 and and CC cord-266018-8bhnlsgy 120 3 E E NNP cord-266018-8bhnlsgy 120 4 ) ) -RRB- cord-266018-8bhnlsgy 120 5 . . . cord-266018-8bhnlsgy 121 1 One one CD cord-266018-8bhnlsgy 121 2 mechanism mechanism NN cord-266018-8bhnlsgy 121 3 by by IN cord-266018-8bhnlsgy 121 4 which which WDT cord-266018-8bhnlsgy 121 5 DCs DCs NNP cord-266018-8bhnlsgy 121 6 influence influence VBP cord-266018-8bhnlsgy 121 7 the the DT cord-266018-8bhnlsgy 121 8 T T NNP cord-266018-8bhnlsgy 121 9 cell cell NN cord-266018-8bhnlsgy 121 10 response response NN cord-266018-8bhnlsgy 121 11 to to IN cord-266018-8bhnlsgy 121 12 infection infection NN cord-266018-8bhnlsgy 121 13 is be VBZ cord-266018-8bhnlsgy 121 14 through through IN cord-266018-8bhnlsgy 121 15 the the DT cord-266018-8bhnlsgy 121 16 secretion secretion NN cord-266018-8bhnlsgy 121 17 of of IN cord-266018-8bhnlsgy 121 18 cytokines cytokine NNS cord-266018-8bhnlsgy 121 19 that that WDT cord-266018-8bhnlsgy 121 20 can can MD cord-266018-8bhnlsgy 121 21 subsequently subsequently RB cord-266018-8bhnlsgy 121 22 polarize polarize VB cord-266018-8bhnlsgy 121 23 the the DT cord-266018-8bhnlsgy 121 24 immune immune JJ cord-266018-8bhnlsgy 121 25 response response NN cord-266018-8bhnlsgy 121 26 towards towards IN cord-266018-8bhnlsgy 121 27 either either CC cord-266018-8bhnlsgy 121 28 a a DT cord-266018-8bhnlsgy 121 29 Th1 th1 NN cord-266018-8bhnlsgy 121 30 or or CC cord-266018-8bhnlsgy 121 31 Th2 th2 CD cord-266018-8bhnlsgy 121 32 phenotype phenotype NN cord-266018-8bhnlsgy 121 33 depending depend VBG cord-266018-8bhnlsgy 121 34 on on IN cord-266018-8bhnlsgy 121 35 the the DT cord-266018-8bhnlsgy 121 36 antigenic antigenic JJ cord-266018-8bhnlsgy 121 37 challenge challenge NN cord-266018-8bhnlsgy 121 38 . . . cord-266018-8bhnlsgy 122 1 To to TO cord-266018-8bhnlsgy 122 2 determine determine VB cord-266018-8bhnlsgy 122 3 if if IN cord-266018-8bhnlsgy 122 4 CD8a CD8a NNP cord-266018-8bhnlsgy 122 5 À À HYPH cord-266018-8bhnlsgy 122 6 DCs dc NNS cord-266018-8bhnlsgy 122 7 present present NN cord-266018-8bhnlsgy 122 8 within within IN cord-266018-8bhnlsgy 122 9 the the DT cord-266018-8bhnlsgy 122 10 CLN cln NN cord-266018-8bhnlsgy 122 11 of of IN cord-266018-8bhnlsgy 122 12 infected infect VBN cord-266018-8bhnlsgy 122 13 CCL3 CCL3 NNP cord-266018-8bhnlsgy 122 14 + + CC cord-266018-8bhnlsgy 122 15 /+ /+ . cord-266018-8bhnlsgy 122 16 and and CC cord-266018-8bhnlsgy 122 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 122 18 À À NNP cord-266018-8bhnlsgy 122 19 / / SYM cord-266018-8bhnlsgy 122 20 À à NN cord-266018-8bhnlsgy 122 21 mice mouse NNS cord-266018-8bhnlsgy 122 22 were be VBD cord-266018-8bhnlsgy 122 23 capable capable JJ cord-266018-8bhnlsgy 122 24 of of IN cord-266018-8bhnlsgy 122 25 secreting secrete VBG cord-266018-8bhnlsgy 122 26 either either CC cord-266018-8bhnlsgy 122 27 chemokines chemokine NNS cord-266018-8bhnlsgy 122 28 or or CC cord-266018-8bhnlsgy 122 29 cytokines cytokine NNS cord-266018-8bhnlsgy 122 30 following follow VBG cord-266018-8bhnlsgy 122 31 MHV MHV NNP cord-266018-8bhnlsgy 122 32 infection infection NN cord-266018-8bhnlsgy 122 33 of of IN cord-266018-8bhnlsgy 122 34 the the DT cord-266018-8bhnlsgy 122 35 CNS CNS NNP cord-266018-8bhnlsgy 122 36 , , , cord-266018-8bhnlsgy 122 37 these these DT cord-266018-8bhnlsgy 122 38 cells cell NNS cord-266018-8bhnlsgy 122 39 were be VBD cord-266018-8bhnlsgy 122 40 isolated isolate VBN cord-266018-8bhnlsgy 122 41 and and CC cord-266018-8bhnlsgy 122 42 production production NN cord-266018-8bhnlsgy 122 43 determined determine VBN cord-266018-8bhnlsgy 122 44 by by IN cord-266018-8bhnlsgy 122 45 ELISA ELISA NNP cord-266018-8bhnlsgy 122 46 . . . cord-266018-8bhnlsgy 123 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 123 2 was be VBD cord-266018-8bhnlsgy 123 3 readily readily RB cord-266018-8bhnlsgy 123 4 detectable detectable JJ cord-266018-8bhnlsgy 123 5 from from IN cord-266018-8bhnlsgy 123 6 CD8a CD8a NNP cord-266018-8bhnlsgy 123 7 À À HYPH cord-266018-8bhnlsgy 123 8 DCs DCs NNP cord-266018-8bhnlsgy 123 9 obtained obtain VBD cord-266018-8bhnlsgy 123 10 from from IN cord-266018-8bhnlsgy 123 11 CCL3 CCL3 NNP cord-266018-8bhnlsgy 123 12 + + CC cord-266018-8bhnlsgy 124 1 /+ /+ NFP cord-266018-8bhnlsgy 124 2 mice mouse NNS cord-266018-8bhnlsgy 124 3 while while IN cord-266018-8bhnlsgy 124 4 CCL3 CCL3 NNP cord-266018-8bhnlsgy 124 5 was be VBD cord-266018-8bhnlsgy 124 6 not not RB cord-266018-8bhnlsgy 124 7 detected detect VBN cord-266018-8bhnlsgy 124 8 in in IN cord-266018-8bhnlsgy 124 9 supernatants supernatant NNS cord-266018-8bhnlsgy 124 10 collected collect VBN cord-266018-8bhnlsgy 124 11 from from IN cord-266018-8bhnlsgy 124 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 124 13 À À NNP cord-266018-8bhnlsgy 124 14 / / SYM cord-266018-8bhnlsgy 124 15 À à FW cord-266018-8bhnlsgy 124 16 CD8a CD8a NNP cord-266018-8bhnlsgy 124 17 À À HYPH cord-266018-8bhnlsgy 124 18 DCs dc NNS cord-266018-8bhnlsgy 124 19 ( ( -LRB- cord-266018-8bhnlsgy 124 20 Fig Fig NNP cord-266018-8bhnlsgy 124 21 . . . cord-266018-8bhnlsgy 124 22 3 3 CD cord-266018-8bhnlsgy 124 23 ) ) -RRB- cord-266018-8bhnlsgy 124 24 . . . cord-266018-8bhnlsgy 125 1 The the DT cord-266018-8bhnlsgy 125 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 125 3 + + CC cord-266018-8bhnlsgy 125 4 /+ /+ . cord-266018-8bhnlsgy 126 1 CD8a CD8a NNP cord-266018-8bhnlsgy 126 2 À À NNP cord-266018-8bhnlsgy 126 3 DC DC NNP cord-266018-8bhnlsgy 126 4 population population NN cord-266018-8bhnlsgy 126 5 secreted secrete VBD cord-266018-8bhnlsgy 126 6 IL-12p70 IL-12p70 NNP cord-266018-8bhnlsgy 126 7 with with IN cord-266018-8bhnlsgy 126 8 low low JJ cord-266018-8bhnlsgy 126 9 - - HYPH cord-266018-8bhnlsgy 126 10 level level NN cord-266018-8bhnlsgy 126 11 production production NN cord-266018-8bhnlsgy 126 12 of of IN cord-266018-8bhnlsgy 126 13 IL-10 IL-10 NNP cord-266018-8bhnlsgy 126 14 ( ( -LRB- cord-266018-8bhnlsgy 126 15 Fig Fig NNP cord-266018-8bhnlsgy 126 16 . . . cord-266018-8bhnlsgy 126 17 3 3 CD cord-266018-8bhnlsgy 126 18 ) ) -RRB- cord-266018-8bhnlsgy 126 19 . . . cord-266018-8bhnlsgy 127 1 In in IN cord-266018-8bhnlsgy 127 2 contrast contrast NN cord-266018-8bhnlsgy 127 3 , , , cord-266018-8bhnlsgy 127 4 the the DT cord-266018-8bhnlsgy 127 5 CD11c CD11c NNP cord-266018-8bhnlsgy 127 6 + + SYM cord-266018-8bhnlsgy 127 7 CD11b CD11b NNS cord-266018-8bhnlsgy 127 8 + + CC cord-266018-8bhnlsgy 127 9 CD8a CD8a NNP cord-266018-8bhnlsgy 127 10 À à NN cord-266018-8bhnlsgy 127 11 cells cell NNS cord-266018-8bhnlsgy 127 12 isolated isolate VBN cord-266018-8bhnlsgy 127 13 from from IN cord-266018-8bhnlsgy 127 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 127 15 À À NNP cord-266018-8bhnlsgy 127 16 / / SYM cord-266018-8bhnlsgy 127 17 À à NN cord-266018-8bhnlsgy 127 18 mice mouse NNS cord-266018-8bhnlsgy 127 19 secreted secrete VBD cord-266018-8bhnlsgy 127 20 approximately approximately RB cord-266018-8bhnlsgy 127 21 3-fold 3-fold CD cord-266018-8bhnlsgy 127 22 less less JJR cord-266018-8bhnlsgy 127 23 IL-12p70 il-12p70 NN cord-266018-8bhnlsgy 127 24 while while IN cord-266018-8bhnlsgy 127 25 IL-10 IL-10 NNP cord-266018-8bhnlsgy 127 26 secretion secretion NN cord-266018-8bhnlsgy 127 27 was be VBD cord-266018-8bhnlsgy 127 28 increased increase VBN cord-266018-8bhnlsgy 127 29 by by IN cord-266018-8bhnlsgy 127 30 5-fold 5-fold CD cord-266018-8bhnlsgy 127 31 as as IN cord-266018-8bhnlsgy 127 32 compared compare VBN cord-266018-8bhnlsgy 127 33 to to IN cord-266018-8bhnlsgy 127 34 cells cell NNS cord-266018-8bhnlsgy 127 35 from from IN cord-266018-8bhnlsgy 127 36 CCL3 CCL3 NNP cord-266018-8bhnlsgy 127 37 + + CC cord-266018-8bhnlsgy 127 38 /+ /+ . cord-266018-8bhnlsgy 128 1 mice mouse NNS cord-266018-8bhnlsgy 128 2 ( ( -LRB- cord-266018-8bhnlsgy 128 3 Fig Fig NNP cord-266018-8bhnlsgy 128 4 . . . cord-266018-8bhnlsgy 128 5 3 3 CD cord-266018-8bhnlsgy 128 6 ) ) -RRB- cord-266018-8bhnlsgy 128 7 . . . cord-266018-8bhnlsgy 129 1 Altered altered JJ cord-266018-8bhnlsgy 129 2 cytokine cytokine NN cord-266018-8bhnlsgy 129 3 production production NN cord-266018-8bhnlsgy 129 4 in in IN cord-266018-8bhnlsgy 129 5 CCL3 CCL3 NNP cord-266018-8bhnlsgy 129 6 À À NNP cord-266018-8bhnlsgy 129 7 / / SYM cord-266018-8bhnlsgy 129 8 À à NN cord-266018-8bhnlsgy 129 9 T t NN cord-266018-8bhnlsgy 129 10 cells cell NNS cord-266018-8bhnlsgy 130 1 We -PRON- PRP cord-266018-8bhnlsgy 130 2 next next RB cord-266018-8bhnlsgy 130 3 evaluated evaluate VBD cord-266018-8bhnlsgy 130 4 the the DT cord-266018-8bhnlsgy 130 5 ability ability NN cord-266018-8bhnlsgy 130 6 of of IN cord-266018-8bhnlsgy 130 7 T t NN cord-266018-8bhnlsgy 130 8 cells cell NNS cord-266018-8bhnlsgy 130 9 obtained obtain VBN cord-266018-8bhnlsgy 130 10 from from IN cord-266018-8bhnlsgy 130 11 the the DT cord-266018-8bhnlsgy 130 12 CLN cln NN cord-266018-8bhnlsgy 130 13 of of IN cord-266018-8bhnlsgy 130 14 either either DT cord-266018-8bhnlsgy 130 15 MHV MHV NNP cord-266018-8bhnlsgy 130 16 - - HYPH cord-266018-8bhnlsgy 130 17 infected infect VBN cord-266018-8bhnlsgy 130 18 CCL3 CCL3 NNP cord-266018-8bhnlsgy 130 19 + + CC cord-266018-8bhnlsgy 130 20 /+ /+ , cord-266018-8bhnlsgy 130 21 or or CC cord-266018-8bhnlsgy 130 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 130 23 À À NNP cord-266018-8bhnlsgy 130 24 / / SYM cord-266018-8bhnlsgy 130 25 À à NN cord-266018-8bhnlsgy 130 26 mice mouse NNS cord-266018-8bhnlsgy 130 27 to to TO cord-266018-8bhnlsgy 130 28 synthesize synthesize VB cord-266018-8bhnlsgy 130 29 cytokines cytokine NNS cord-266018-8bhnlsgy 130 30 following follow VBG cord-266018-8bhnlsgy 130 31 exposure exposure NN cord-266018-8bhnlsgy 130 32 to to IN cord-266018-8bhnlsgy 130 33 defined define VBN cord-266018-8bhnlsgy 130 34 viral viral JJ cord-266018-8bhnlsgy 130 35 antigens antigen NNS cord-266018-8bhnlsgy 130 36 . . . cord-266018-8bhnlsgy 131 1 T t NN cord-266018-8bhnlsgy 131 2 cells cell NNS cord-266018-8bhnlsgy 131 3 were be VBD cord-266018-8bhnlsgy 131 4 isolated isolate VBN cord-266018-8bhnlsgy 131 5 from from IN cord-266018-8bhnlsgy 131 6 the the DT cord-266018-8bhnlsgy 131 7 CLN cln NN cord-266018-8bhnlsgy 131 8 of of IN cord-266018-8bhnlsgy 131 9 CCL3 CCL3 NNP cord-266018-8bhnlsgy 131 10 + + CC cord-266018-8bhnlsgy 131 11 /+ /+ . cord-266018-8bhnlsgy 132 1 and and CC cord-266018-8bhnlsgy 132 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 132 3 À À NNP cord-266018-8bhnlsgy 132 4 / / SYM cord-266018-8bhnlsgy 132 5 À à NN cord-266018-8bhnlsgy 132 6 mice mouse NNS cord-266018-8bhnlsgy 132 7 at at IN cord-266018-8bhnlsgy 132 8 days day NNS cord-266018-8bhnlsgy 132 9 7 7 CD cord-266018-8bhnlsgy 132 10 and and CC cord-266018-8bhnlsgy 132 11 12 12 CD cord-266018-8bhnlsgy 132 12 p.i p.i NNP cord-266018-8bhnlsgy 132 13 . . . cord-266018-8bhnlsgy 133 1 following follow VBG cord-266018-8bhnlsgy 133 2 intracranial intracranial JJ cord-266018-8bhnlsgy 133 3 infection infection NN cord-266018-8bhnlsgy 133 4 with with IN cord-266018-8bhnlsgy 133 5 MHV MHV NNP cord-266018-8bhnlsgy 133 6 and and CC cord-266018-8bhnlsgy 133 7 stimulated stimulate VBN cord-266018-8bhnlsgy 133 8 with with IN cord-266018-8bhnlsgy 133 9 peptides peptide NNS cord-266018-8bhnlsgy 133 10 corresponding correspond VBG cord-266018-8bhnlsgy 133 11 to to IN cord-266018-8bhnlsgy 133 12 either either CC cord-266018-8bhnlsgy 133 13 the the DT cord-266018-8bhnlsgy 133 14 immunodominant immunodominant NN cord-266018-8bhnlsgy 133 15 CD4 CD4 NNP cord-266018-8bhnlsgy 133 16 epitope epitope NNP cord-266018-8bhnlsgy 133 17 present present JJ cord-266018-8bhnlsgy 133 18 within within IN cord-266018-8bhnlsgy 133 19 the the DT cord-266018-8bhnlsgy 133 20 matrix matrix NN cord-266018-8bhnlsgy 133 21 ( ( -LRB- cord-266018-8bhnlsgy 133 22 M M NNP cord-266018-8bhnlsgy 133 23 ) ) -RRB- cord-266018-8bhnlsgy 133 24 glycoprotein glycoprotein NN cord-266018-8bhnlsgy 133 25 at at IN cord-266018-8bhnlsgy 133 26 residues residue NNS cord-266018-8bhnlsgy 133 27 133 133 CD cord-266018-8bhnlsgy 133 28 - - SYM cord-266018-8bhnlsgy 133 29 147 147 CD cord-266018-8bhnlsgy 133 30 ( ( -LRB- cord-266018-8bhnlsgy 133 31 M133 M133 NNP cord-266018-8bhnlsgy 133 32 - - HYPH cord-266018-8bhnlsgy 133 33 147 147 CD cord-266018-8bhnlsgy 133 34 ) ) -RRB- cord-266018-8bhnlsgy 133 35 or or CC cord-266018-8bhnlsgy 133 36 the the DT cord-266018-8bhnlsgy 133 37 immunodominant immunodominant JJ cord-266018-8bhnlsgy 133 38 CD8 CD8 NNP cord-266018-8bhnlsgy 133 39 epitope epitope NNP cord-266018-8bhnlsgy 133 40 in in IN cord-266018-8bhnlsgy 133 41 the the DT cord-266018-8bhnlsgy 133 42 surface surface NN cord-266018-8bhnlsgy 133 43 ( ( -LRB- cord-266018-8bhnlsgy 133 44 S s NN cord-266018-8bhnlsgy 133 45 ) ) -RRB- cord-266018-8bhnlsgy 133 46 glycoprotein glycoprotein NN cord-266018-8bhnlsgy 133 47 spanning span VBG cord-266018-8bhnlsgy 133 48 residues residue NNS cord-266018-8bhnlsgy 133 49 510 510 CD cord-266018-8bhnlsgy 133 50 - - HYPH cord-266018-8bhnlsgy 133 51 518 518 CD cord-266018-8bhnlsgy 134 1 ( ( -LRB- cord-266018-8bhnlsgy 134 2 S510 S510 NNP cord-266018-8bhnlsgy 134 3 - - HYPH cord-266018-8bhnlsgy 134 4 518 518 CD cord-266018-8bhnlsgy 134 5 ) ) -RRB- cord-266018-8bhnlsgy 134 6 and and CC cord-266018-8bhnlsgy 134 7 cytokine cytokine NN cord-266018-8bhnlsgy 134 8 production production NN cord-266018-8bhnlsgy 134 9 by by IN cord-266018-8bhnlsgy 134 10 T t NN cord-266018-8bhnlsgy 134 11 cells cell NNS cord-266018-8bhnlsgy 134 12 determined determine VBN cord-266018-8bhnlsgy 134 13 by by IN cord-266018-8bhnlsgy 134 14 intracellular intracellular JJ cord-266018-8bhnlsgy 134 15 cytokine cytokine NN cord-266018-8bhnlsgy 134 16 staining staining NN cord-266018-8bhnlsgy 134 17 ( ( -LRB- cord-266018-8bhnlsgy 134 18 Castro Castro NNP cord-266018-8bhnlsgy 134 19 and and CC cord-266018-8bhnlsgy 134 20 Perlman Perlman NNP cord-266018-8bhnlsgy 134 21 , , , cord-266018-8bhnlsgy 134 22 1995 1995 CD cord-266018-8bhnlsgy 134 23 ; ; : cord-266018-8bhnlsgy 134 24 Xue Xue NNP cord-266018-8bhnlsgy 134 25 et et NNP cord-266018-8bhnlsgy 134 26 al al NNP cord-266018-8bhnlsgy 134 27 . . NNP cord-266018-8bhnlsgy 134 28 , , , cord-266018-8bhnlsgy 134 29 1995 1995 CD cord-266018-8bhnlsgy 134 30 ) ) -RRB- cord-266018-8bhnlsgy 134 31 . . . cord-266018-8bhnlsgy 135 1 The the DT cord-266018-8bhnlsgy 135 2 results result NNS cord-266018-8bhnlsgy 135 3 presented present VBN cord-266018-8bhnlsgy 135 4 in in IN cord-266018-8bhnlsgy 135 5 Table Table NNP cord-266018-8bhnlsgy 135 6 1 1 CD cord-266018-8bhnlsgy 135 7 indicate indicate VBP cord-266018-8bhnlsgy 135 8 similar similar JJ cord-266018-8bhnlsgy 135 9 frequencies frequency NNS cord-266018-8bhnlsgy 135 10 of of IN cord-266018-8bhnlsgy 135 11 CD4 CD4 NNP cord-266018-8bhnlsgy 135 12 + + CC cord-266018-8bhnlsgy 135 13 and and CC cord-266018-8bhnlsgy 135 14 CD8 CD8 NNP cord-266018-8bhnlsgy 135 15 + + CC cord-266018-8bhnlsgy 135 16 T T NNP cord-266018-8bhnlsgy 135 17 cells cell NNS cord-266018-8bhnlsgy 135 18 from from IN cord-266018-8bhnlsgy 135 19 CCL3 CCL3 NNP cord-266018-8bhnlsgy 135 20 + + CC cord-266018-8bhnlsgy 135 21 /+ /+ . cord-266018-8bhnlsgy 136 1 mice mouse NNS cord-266018-8bhnlsgy 136 2 produced produce VBD cord-266018-8bhnlsgy 136 3 IFN IFN NNP cord-266018-8bhnlsgy 136 4 - - HYPH cord-266018-8bhnlsgy 136 5 g g NNP cord-266018-8bhnlsgy 136 6 at at IN cord-266018-8bhnlsgy 136 7 days day NNS cord-266018-8bhnlsgy 136 8 7 7 CD cord-266018-8bhnlsgy 136 9 and and CC cord-266018-8bhnlsgy 136 10 12 12 CD cord-266018-8bhnlsgy 136 11 p.i p.i NNP cord-266018-8bhnlsgy 136 12 . . . cord-266018-8bhnlsgy 137 1 Both both DT cord-266018-8bhnlsgy 137 2 CD4 CD4 NNP cord-266018-8bhnlsgy 137 3 + + CC cord-266018-8bhnlsgy 137 4 and and CC cord-266018-8bhnlsgy 137 5 CD8 CD8 NNP cord-266018-8bhnlsgy 137 6 + + CC cord-266018-8bhnlsgy 137 7 CCL3 CCL3 NNP cord-266018-8bhnlsgy 137 8 + + CC cord-266018-8bhnlsgy 137 9 /+ /+ . cord-266018-8bhnlsgy 138 1 T t NN cord-266018-8bhnlsgy 138 2 cells cell NNS cord-266018-8bhnlsgy 138 3 also also RB cord-266018-8bhnlsgy 138 4 secreted secrete VBD cord-266018-8bhnlsgy 138 5 IL-2 il-2 NN cord-266018-8bhnlsgy 138 6 following follow VBG cord-266018-8bhnlsgy 138 7 specific specific JJ cord-266018-8bhnlsgy 138 8 peptide peptide JJ cord-266018-8bhnlsgy 138 9 exposure exposure NN cord-266018-8bhnlsgy 138 10 at at IN cord-266018-8bhnlsgy 138 11 day day NN cord-266018-8bhnlsgy 138 12 7 7 CD cord-266018-8bhnlsgy 138 13 , , , cord-266018-8bhnlsgy 138 14 although although IN cord-266018-8bhnlsgy 138 15 expression expression NN cord-266018-8bhnlsgy 138 16 was be VBD cord-266018-8bhnlsgy 138 17 limited limit VBN cord-266018-8bhnlsgy 138 18 to to IN cord-266018-8bhnlsgy 138 19 the the DT cord-266018-8bhnlsgy 138 20 acute acute JJ cord-266018-8bhnlsgy 138 21 stage stage NN cord-266018-8bhnlsgy 138 22 of of IN cord-266018-8bhnlsgy 138 23 MHV MHV NNP cord-266018-8bhnlsgy 138 24 infection infection NN cord-266018-8bhnlsgy 138 25 as as IN cord-266018-8bhnlsgy 138 26 the the DT cord-266018-8bhnlsgy 138 27 frequency frequency NN cord-266018-8bhnlsgy 138 28 of of IN cord-266018-8bhnlsgy 138 29 IL-2producing il-2producing NN cord-266018-8bhnlsgy 138 30 cells cell NNS cord-266018-8bhnlsgy 138 31 was be VBD cord-266018-8bhnlsgy 138 32 reduced reduce VBN cord-266018-8bhnlsgy 138 33 at at IN cord-266018-8bhnlsgy 138 34 day day NN cord-266018-8bhnlsgy 138 35 12 12 CD cord-266018-8bhnlsgy 138 36 p.i p.i NNP cord-266018-8bhnlsgy 138 37 . . . cord-266018-8bhnlsgy 139 1 ( ( -LRB- cord-266018-8bhnlsgy 139 2 Table table NN cord-266018-8bhnlsgy 139 3 1 1 CD cord-266018-8bhnlsgy 139 4 ) ) -RRB- cord-266018-8bhnlsgy 139 5 . . . cord-266018-8bhnlsgy 140 1 In in IN cord-266018-8bhnlsgy 140 2 contrast contrast NN cord-266018-8bhnlsgy 140 3 , , , cord-266018-8bhnlsgy 140 4 the the DT cord-266018-8bhnlsgy 140 5 frequency frequency NN cord-266018-8bhnlsgy 140 6 of of IN cord-266018-8bhnlsgy 140 7 CD4 CD4 NNP cord-266018-8bhnlsgy 140 8 + + CC cord-266018-8bhnlsgy 140 9 and and CC cord-266018-8bhnlsgy 140 10 CD8 CD8 NNP cord-266018-8bhnlsgy 140 11 + + CC cord-266018-8bhnlsgy 140 12 T T NNP cord-266018-8bhnlsgy 140 13 cells cell NNS cord-266018-8bhnlsgy 140 14 isolated isolate VBN cord-266018-8bhnlsgy 140 15 from from IN cord-266018-8bhnlsgy 140 16 CCL3 CCL3 NNP cord-266018-8bhnlsgy 140 17 À à NN cord-266018-8bhnlsgy 140 18 / / SYM cord-266018-8bhnlsgy 140 19 À à NN cord-266018-8bhnlsgy 140 20 mice mouse NNS cord-266018-8bhnlsgy 140 21 secreting secrete VBG cord-266018-8bhnlsgy 140 22 IFN IFN NNP cord-266018-8bhnlsgy 140 23 - - HYPH cord-266018-8bhnlsgy 140 24 g g NNP cord-266018-8bhnlsgy 140 25 following follow VBG cord-266018-8bhnlsgy 140 26 peptide peptide JJ cord-266018-8bhnlsgy 140 27 stimulation stimulation NN cord-266018-8bhnlsgy 140 28 was be VBD cord-266018-8bhnlsgy 140 29 dramatically dramatically RB cord-266018-8bhnlsgy 140 30 reduced reduce VBN cord-266018-8bhnlsgy 140 31 . . . cord-266018-8bhnlsgy 141 1 Expression expression NN cord-266018-8bhnlsgy 141 2 of of IN cord-266018-8bhnlsgy 141 3 IL-2 IL-2 NNP cord-266018-8bhnlsgy 141 4 by by IN cord-266018-8bhnlsgy 141 5 CCL3 CCL3 NNP cord-266018-8bhnlsgy 141 6 À À NNP cord-266018-8bhnlsgy 141 7 / / SYM cord-266018-8bhnlsgy 141 8 À à NN cord-266018-8bhnlsgy 141 9 T t NN cord-266018-8bhnlsgy 141 10 cells cell NNS cord-266018-8bhnlsgy 141 11 was be VBD cord-266018-8bhnlsgy 141 12 comparable comparable JJ cord-266018-8bhnlsgy 141 13 with with IN cord-266018-8bhnlsgy 141 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 141 15 + + CC cord-266018-8bhnlsgy 141 16 /+ /+ . cord-266018-8bhnlsgy 142 1 mice mouse NNS cord-266018-8bhnlsgy 142 2 at at IN cord-266018-8bhnlsgy 142 3 day day NN cord-266018-8bhnlsgy 143 1 7 7 CD cord-266018-8bhnlsgy 143 2 p.i p.i NNP cord-266018-8bhnlsgy 143 3 . . . cord-266018-8bhnlsgy 144 1 However however RB cord-266018-8bhnlsgy 144 2 , , , cord-266018-8bhnlsgy 144 3 by by IN cord-266018-8bhnlsgy 144 4 day day NN cord-266018-8bhnlsgy 144 5 12 12 CD cord-266018-8bhnlsgy 144 6 p.i p.i NNP cord-266018-8bhnlsgy 144 7 . . NNP cord-266018-8bhnlsgy 144 8 , , , cord-266018-8bhnlsgy 144 9 the the DT cord-266018-8bhnlsgy 144 10 frequency frequency NN cord-266018-8bhnlsgy 144 11 of of IN cord-266018-8bhnlsgy 144 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 144 13 À à NN cord-266018-8bhnlsgy 144 14 / / SYM cord-266018-8bhnlsgy 144 15 À à NN cord-266018-8bhnlsgy 144 16 T t NN cord-266018-8bhnlsgy 144 17 cells cell NNS cord-266018-8bhnlsgy 144 18 expressing express VBG cord-266018-8bhnlsgy 144 19 IL-2 IL-2 NNP cord-266018-8bhnlsgy 144 20 remained remain VBD cord-266018-8bhnlsgy 144 21 elevated elevated JJ cord-266018-8bhnlsgy 144 22 as as IN cord-266018-8bhnlsgy 144 23 compared compare VBN cord-266018-8bhnlsgy 144 24 to to IN cord-266018-8bhnlsgy 144 25 CCL3 CCL3 NNP cord-266018-8bhnlsgy 144 26 + + CC cord-266018-8bhnlsgy 144 27 /+ /+ . cord-266018-8bhnlsgy 145 1 T t NN cord-266018-8bhnlsgy 145 2 cells cell NNS cord-266018-8bhnlsgy 145 3 ( ( -LRB- cord-266018-8bhnlsgy 145 4 Table table NN cord-266018-8bhnlsgy 145 5 1 1 CD cord-266018-8bhnlsgy 145 6 ) ) -RRB- cord-266018-8bhnlsgy 145 7 . . . cord-266018-8bhnlsgy 146 1 In in IN cord-266018-8bhnlsgy 146 2 addition addition NN cord-266018-8bhnlsgy 146 3 , , , cord-266018-8bhnlsgy 146 4 only only RB cord-266018-8bhnlsgy 146 5 limited limited JJ cord-266018-8bhnlsgy 146 6 frequencies frequency NNS cord-266018-8bhnlsgy 146 7 of of IN cord-266018-8bhnlsgy 146 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 146 9 + + CC cord-266018-8bhnlsgy 146 10 /+ /+ NNP cord-266018-8bhnlsgy 146 11 CD4 CD4 NNP cord-266018-8bhnlsgy 146 12 + + CC cord-266018-8bhnlsgy 146 13 and and CC cord-266018-8bhnlsgy 146 14 CD8 CD8 NNP cord-266018-8bhnlsgy 146 15 + + CC cord-266018-8bhnlsgy 146 16 T T NNP cord-266018-8bhnlsgy 146 17 cells cell NNS cord-266018-8bhnlsgy 146 18 produced produce VBD cord-266018-8bhnlsgy 146 19 IL-10 IL-10 NNP cord-266018-8bhnlsgy 146 20 following follow VBG cord-266018-8bhnlsgy 146 21 MHV MHV NNP cord-266018-8bhnlsgy 146 22 infection infection NN cord-266018-8bhnlsgy 146 23 whereas whereas IN cord-266018-8bhnlsgy 146 24 CCL3 CCL3 NNP cord-266018-8bhnlsgy 146 25 À à NN cord-266018-8bhnlsgy 146 26 / / SYM cord-266018-8bhnlsgy 146 27 À À NNP cord-266018-8bhnlsgy 146 28 CD4 CD4 NNP cord-266018-8bhnlsgy 146 29 + + CC cord-266018-8bhnlsgy 146 30 and and CC cord-266018-8bhnlsgy 146 31 CD8 CD8 NNP cord-266018-8bhnlsgy 146 32 + + CC cord-266018-8bhnlsgy 146 33 T T NNP cord-266018-8bhnlsgy 146 34 cells cell NNS cord-266018-8bhnlsgy 146 35 displayed display VBD cord-266018-8bhnlsgy 146 36 an an DT cord-266018-8bhnlsgy 146 37 overall overall JJ cord-266018-8bhnlsgy 146 38 increase increase NN cord-266018-8bhnlsgy 146 39 in in IN cord-266018-8bhnlsgy 146 40 the the DT cord-266018-8bhnlsgy 146 41 frequency frequency NN cord-266018-8bhnlsgy 146 42 of of IN cord-266018-8bhnlsgy 146 43 IL-10 IL-10 NNP cord-266018-8bhnlsgy 146 44 ( ( -LRB- cord-266018-8bhnlsgy 146 45 Table Table NNP cord-266018-8bhnlsgy 146 46 1 1 CD cord-266018-8bhnlsgy 146 47 ) ) -RRB- cord-266018-8bhnlsgy 146 48 . . . cord-266018-8bhnlsgy 147 1 The the DT cord-266018-8bhnlsgy 147 2 major major JJ cord-266018-8bhnlsgy 147 3 findings finding NNS cord-266018-8bhnlsgy 147 4 of of IN cord-266018-8bhnlsgy 147 5 this this DT cord-266018-8bhnlsgy 147 6 study study NN cord-266018-8bhnlsgy 147 7 are be VBP cord-266018-8bhnlsgy 147 8 ( ( -LRB- cord-266018-8bhnlsgy 147 9 i i NN cord-266018-8bhnlsgy 147 10 ) ) -RRB- cord-266018-8bhnlsgy 148 1 MHV mhv NN cord-266018-8bhnlsgy 148 2 infection infection NN cord-266018-8bhnlsgy 148 3 of of IN cord-266018-8bhnlsgy 148 4 the the DT cord-266018-8bhnlsgy 148 5 CNS CNS NNP cord-266018-8bhnlsgy 148 6 results result NNS cord-266018-8bhnlsgy 148 7 in in IN cord-266018-8bhnlsgy 148 8 the the DT cord-266018-8bhnlsgy 148 9 appearance appearance NN cord-266018-8bhnlsgy 148 10 of of IN cord-266018-8bhnlsgy 148 11 two two CD cord-266018-8bhnlsgy 148 12 distinct distinct JJ cord-266018-8bhnlsgy 148 13 populations population NNS cord-266018-8bhnlsgy 148 14 of of IN cord-266018-8bhnlsgy 148 15 CD11c CD11c NNP cord-266018-8bhnlsgy 149 1 + + CC cord-266018-8bhnlsgy 149 2 cells cell NNS cord-266018-8bhnlsgy 149 3 each each DT cord-266018-8bhnlsgy 149 4 expressing express VBG cord-266018-8bhnlsgy 149 5 markers marker NNS cord-266018-8bhnlsgy 149 6 characteristic characteristic JJ cord-266018-8bhnlsgy 149 7 of of IN cord-266018-8bhnlsgy 149 8 lymphoid lymphoid NN cord-266018-8bhnlsgy 150 1 ( ( -LRB- cord-266018-8bhnlsgy 150 2 CD11c CD11c NNP cord-266018-8bhnlsgy 150 3 + + SYM cord-266018-8bhnlsgy 150 4 CD11b CD11b NNS cord-266018-8bhnlsgy 150 5 À à NN cord-266018-8bhnlsgy 150 6 CD8a CD8a NNP cord-266018-8bhnlsgy 151 1 + + CC cord-266018-8bhnlsgy 152 1 DEC205 DEC205 NNP cord-266018-8bhnlsgy 152 2 + + CC cord-266018-8bhnlsgy 152 3 ) ) -RRB- cord-266018-8bhnlsgy 152 4 and and CC cord-266018-8bhnlsgy 152 5 myeloid myeloid JJ cord-266018-8bhnlsgy 152 6 dendritic dendritic JJ cord-266018-8bhnlsgy 152 7 cells cell NNS cord-266018-8bhnlsgy 152 8 ( ( -LRB- cord-266018-8bhnlsgy 152 9 CD11c CD11c NNP cord-266018-8bhnlsgy 152 10 + + SYM cord-266018-8bhnlsgy 152 11 CD11b CD11b NNS cord-266018-8bhnlsgy 152 12 + + CC cord-266018-8bhnlsgy 152 13 CD8a CD8a NNP cord-266018-8bhnlsgy 152 14 À À NNP cord-266018-8bhnlsgy 152 15 DEC205 DEC205 NNP cord-266018-8bhnlsgy 152 16 À à NN cord-266018-8bhnlsgy 152 17 ) ) -RRB- cord-266018-8bhnlsgy 152 18 , , . cord-266018-8bhnlsgy 153 1 ( ( -LRB- cord-266018-8bhnlsgy 153 2 ii ii NN cord-266018-8bhnlsgy 153 3 ) ) -RRB- cord-266018-8bhnlsgy 154 1 the the DT cord-266018-8bhnlsgy 154 2 accumulation accumulation NN cord-266018-8bhnlsgy 154 3 of of IN cord-266018-8bhnlsgy 154 4 CD8a CD8a NNP cord-266018-8bhnlsgy 154 5 À À HYPH cord-266018-8bhnlsgy 154 6 DCs dc NNS cord-266018-8bhnlsgy 154 7 within within IN cord-266018-8bhnlsgy 154 8 the the DT cord-266018-8bhnlsgy 154 9 draining drain VBG cord-266018-8bhnlsgy 154 10 CLN cln NN cord-266018-8bhnlsgy 154 11 is be VBZ cord-266018-8bhnlsgy 154 12 reduced reduce VBN cord-266018-8bhnlsgy 154 13 in in IN cord-266018-8bhnlsgy 154 14 the the DT cord-266018-8bhnlsgy 154 15 absence absence NN cord-266018-8bhnlsgy 154 16 of of IN cord-266018-8bhnlsgy 154 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 154 18 signaling signaling NN cord-266018-8bhnlsgy 154 19 , , , cord-266018-8bhnlsgy 154 20 ( ( -LRB- cord-266018-8bhnlsgy 154 21 iii iii NN cord-266018-8bhnlsgy 154 22 ) ) -RRB- cord-266018-8bhnlsgy 154 23 expression expression NN cord-266018-8bhnlsgy 154 24 of of IN cord-266018-8bhnlsgy 154 25 co co JJ cord-266018-8bhnlsgy 154 26 - - JJ cord-266018-8bhnlsgy 154 27 stimulatory stimulatory JJ cord-266018-8bhnlsgy 154 28 molecules molecule NNS cord-266018-8bhnlsgy 154 29 such such JJ cord-266018-8bhnlsgy 154 30 as as IN cord-266018-8bhnlsgy 154 31 CD40 CD40 NNP cord-266018-8bhnlsgy 154 32 by by IN cord-266018-8bhnlsgy 154 33 CD8a CD8a NNP cord-266018-8bhnlsgy 154 34 À À HYPH cord-266018-8bhnlsgy 154 35 DCs dc NNS cord-266018-8bhnlsgy 154 36 within within IN cord-266018-8bhnlsgy 154 37 either either CC cord-266018-8bhnlsgy 154 38 the the DT cord-266018-8bhnlsgy 154 39 brain brain NN cord-266018-8bhnlsgy 154 40 and and CC cord-266018-8bhnlsgy 154 41 CLN cln NN cord-266018-8bhnlsgy 154 42 of of IN cord-266018-8bhnlsgy 154 43 MHV MHV NNP cord-266018-8bhnlsgy 154 44 - - HYPH cord-266018-8bhnlsgy 154 45 infected infect VBN cord-266018-8bhnlsgy 154 46 CCL3 CCL3 NNP cord-266018-8bhnlsgy 154 47 À à NN cord-266018-8bhnlsgy 154 48 / / SYM cord-266018-8bhnlsgy 154 49 À à NN cord-266018-8bhnlsgy 154 50 mice mouse NNS cord-266018-8bhnlsgy 154 51 is be VBZ cord-266018-8bhnlsgy 154 52 diminished diminish VBN cord-266018-8bhnlsgy 154 53 suggesting suggest VBG cord-266018-8bhnlsgy 154 54 that that IN cord-266018-8bhnlsgy 154 55 CCL3 CCL3 NNP cord-266018-8bhnlsgy 154 56 signaling signaling NN cord-266018-8bhnlsgy 154 57 enhances enhance VBZ cord-266018-8bhnlsgy 154 58 expression expression NN cord-266018-8bhnlsgy 154 59 of of IN cord-266018-8bhnlsgy 154 60 these these DT cord-266018-8bhnlsgy 154 61 molecules molecule NNS cord-266018-8bhnlsgy 154 62 , , , cord-266018-8bhnlsgy 154 63 and and CC cord-266018-8bhnlsgy 154 64 ( ( -LRB- cord-266018-8bhnlsgy 154 65 iv iv LS cord-266018-8bhnlsgy 154 66 ) ) -RRB- cord-266018-8bhnlsgy 154 67 absence absence NN cord-266018-8bhnlsgy 154 68 of of IN cord-266018-8bhnlsgy 154 69 CCL3 CCL3 NNP cord-266018-8bhnlsgy 154 70 signaling signal VBG cord-266018-8bhnlsgy 154 71 results result NNS cord-266018-8bhnlsgy 154 72 in in IN cord-266018-8bhnlsgy 154 73 the the DT cord-266018-8bhnlsgy 154 74 re re NN cord-266018-8bhnlsgy 154 75 - - NN cord-266018-8bhnlsgy 154 76 direction direction NN cord-266018-8bhnlsgy 154 77 of of IN cord-266018-8bhnlsgy 154 78 the the DT cord-266018-8bhnlsgy 154 79 T T NNP cord-266018-8bhnlsgy 154 80 cell cell NN cord-266018-8bhnlsgy 154 81 response response NN cord-266018-8bhnlsgy 154 82 to to IN cord-266018-8bhnlsgy 154 83 viral viral JJ cord-266018-8bhnlsgy 154 84 antigens antigen NNS cord-266018-8bhnlsgy 154 85 as as IN cord-266018-8bhnlsgy 154 86 determined determine VBN cord-266018-8bhnlsgy 154 87 by by IN cord-266018-8bhnlsgy 154 88 cytokine cytokine NN cord-266018-8bhnlsgy 154 89 production production NN cord-266018-8bhnlsgy 154 90 . . . cord-266018-8bhnlsgy 155 1 These these DT cord-266018-8bhnlsgy 155 2 data datum NNS cord-266018-8bhnlsgy 155 3 support support NN cord-266018-8bhnlsgy 155 4 and and CC cord-266018-8bhnlsgy 155 5 extend extend VB cord-266018-8bhnlsgy 155 6 recent recent JJ cord-266018-8bhnlsgy 155 7 studies study NNS cord-266018-8bhnlsgy 155 8 from from IN cord-266018-8bhnlsgy 155 9 our -PRON- PRP$ cord-266018-8bhnlsgy 155 10 laboratory laboratory NN cord-266018-8bhnlsgy 155 11 demonstrating demonstrate VBG cord-266018-8bhnlsgy 155 12 an an DT cord-266018-8bhnlsgy 155 13 important important JJ cord-266018-8bhnlsgy 155 14 role role NN cord-266018-8bhnlsgy 155 15 for for IN cord-266018-8bhnlsgy 155 16 CCL3 CCL3 NNP cord-266018-8bhnlsgy 155 17 in in IN cord-266018-8bhnlsgy 155 18 generating generate VBG cord-266018-8bhnlsgy 155 19 effector effector JJ cord-266018-8bhnlsgy 155 20 anti anti JJ cord-266018-8bhnlsgy 155 21 - - JJ cord-266018-8bhnlsgy 155 22 viral viral JJ cord-266018-8bhnlsgy 155 23 T t NN cord-266018-8bhnlsgy 155 24 cells cell NNS cord-266018-8bhnlsgy 155 25 capable capable JJ cord-266018-8bhnlsgy 155 26 of of IN cord-266018-8bhnlsgy 155 27 migrating migrate VBG cord-266018-8bhnlsgy 155 28 to to IN cord-266018-8bhnlsgy 155 29 the the DT cord-266018-8bhnlsgy 155 30 brain brain NN cord-266018-8bhnlsgy 155 31 in in IN cord-266018-8bhnlsgy 155 32 response response NN cord-266018-8bhnlsgy 155 33 to to IN cord-266018-8bhnlsgy 155 34 viral viral JJ cord-266018-8bhnlsgy 155 35 infection infection NN cord-266018-8bhnlsgy 155 36 ( ( -LRB- cord-266018-8bhnlsgy 155 37 Trifilo Trifilo NNP cord-266018-8bhnlsgy 155 38 et et FW cord-266018-8bhnlsgy 155 39 al al NNP cord-266018-8bhnlsgy 155 40 . . NNP cord-266018-8bhnlsgy 155 41 , , , cord-266018-8bhnlsgy 155 42 2003 2003 CD cord-266018-8bhnlsgy 155 43 ) ) -RRB- cord-266018-8bhnlsgy 155 44 . . . cord-266018-8bhnlsgy 155 45 i. i. LS cord-266018-8bhnlsgy 155 46 ) ) -RRB- cord-266018-8bhnlsgy 155 47 CCL3 CCL3 NNP cord-266018-8bhnlsgy 155 48 + + CC cord-266018-8bhnlsgy 155 49 /+ /+ . cord-266018-8bhnlsgy 156 1 and and CC cord-266018-8bhnlsgy 156 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 156 3 À À NNP cord-266018-8bhnlsgy 156 4 / / SYM cord-266018-8bhnlsgy 156 5 À à NN cord-266018-8bhnlsgy 156 6 mice mouse NNS cord-266018-8bhnlsgy 156 7 and and CC cord-266018-8bhnlsgy 156 8 stained stain VBN cord-266018-8bhnlsgy 156 9 for for IN cord-266018-8bhnlsgy 156 10 CD11c CD11c NNP cord-266018-8bhnlsgy 157 1 + + CC cord-266018-8bhnlsgy 158 1 and and CC cord-266018-8bhnlsgy 158 2 CD11b CD11b NNS cord-266018-8bhnlsgy 158 3 + + CC cord-266018-8bhnlsgy 158 4 expression expression NN cord-266018-8bhnlsgy 158 5 . . . cord-266018-8bhnlsgy 159 1 Gated gated JJ cord-266018-8bhnlsgy 159 2 populations population NNS cord-266018-8bhnlsgy 159 3 represent represent VBP cord-266018-8bhnlsgy 159 4 CD11c CD11c NNP cord-266018-8bhnlsgy 159 5 + + SYM cord-266018-8bhnlsgy 159 6 CD11b CD11b NNS cord-266018-8bhnlsgy 159 7 + + CC cord-266018-8bhnlsgy 159 8 ( ( -LRB- cord-266018-8bhnlsgy 159 9 upper upper JJ cord-266018-8bhnlsgy 159 10 - - HYPH cord-266018-8bhnlsgy 159 11 right right JJ cord-266018-8bhnlsgy 159 12 quadrant quadrant NN cord-266018-8bhnlsgy 159 13 ) ) -RRB- cord-266018-8bhnlsgy 159 14 or or CC cord-266018-8bhnlsgy 159 15 CD11c CD11c NNP cord-266018-8bhnlsgy 159 16 + + SYM cord-266018-8bhnlsgy 159 17 CD11b CD11b NNS cord-266018-8bhnlsgy 159 18 À à NN cord-266018-8bhnlsgy 159 19 ( ( -LRB- cord-266018-8bhnlsgy 159 20 lower low JJR cord-266018-8bhnlsgy 159 21 - - HYPH cord-266018-8bhnlsgy 159 22 right right JJ cord-266018-8bhnlsgy 159 23 quadrant quadrant NN cord-266018-8bhnlsgy 159 24 ) ) -RRB- cord-266018-8bhnlsgy 159 25 and and CC cord-266018-8bhnlsgy 159 26 numbers number NNS cord-266018-8bhnlsgy 159 27 indicate indicate VBP cord-266018-8bhnlsgy 159 28 frequencies frequency NNS cord-266018-8bhnlsgy 159 29 of of IN cord-266018-8bhnlsgy 159 30 gated gated JJ cord-266018-8bhnlsgy 159 31 cells cell NNS cord-266018-8bhnlsgy 159 32 within within IN cord-266018-8bhnlsgy 159 33 the the DT cord-266018-8bhnlsgy 159 34 isolated isolated JJ cord-266018-8bhnlsgy 159 35 population population NN cord-266018-8bhnlsgy 159 36 . . . cord-266018-8bhnlsgy 160 1 ( ( -LRB- cord-266018-8bhnlsgy 160 2 B b NN cord-266018-8bhnlsgy 160 3 ) ) -RRB- cord-266018-8bhnlsgy 160 4 CD8a CD8a NNP cord-266018-8bhnlsgy 160 5 and and CC cord-266018-8bhnlsgy 160 6 DEC205 DEC205 NNP cord-266018-8bhnlsgy 160 7 expression expression NN cord-266018-8bhnlsgy 160 8 on on IN cord-266018-8bhnlsgy 160 9 CD11c CD11c NNP cord-266018-8bhnlsgy 160 10 + + CC cord-266018-8bhnlsgy 160 11 cells cell NNS cord-266018-8bhnlsgy 160 12 . . . cord-266018-8bhnlsgy 161 1 CD11c CD11c NNP cord-266018-8bhnlsgy 161 2 + + SYM cord-266018-8bhnlsgy 161 3 CD11b CD11b NNS cord-266018-8bhnlsgy 161 4 + + CC cord-266018-8bhnlsgy 161 5 cells cell NNS cord-266018-8bhnlsgy 161 6 from from IN cord-266018-8bhnlsgy 161 7 either either CC cord-266018-8bhnlsgy 161 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 161 9 + + CD cord-266018-8bhnlsgy 162 1 /+ /+ . cord-266018-8bhnlsgy 163 1 or or CC cord-266018-8bhnlsgy 163 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 163 3 À À NNP cord-266018-8bhnlsgy 163 4 / / SYM cord-266018-8bhnlsgy 163 5 À à NN cord-266018-8bhnlsgy 163 6 at at IN cord-266018-8bhnlsgy 163 7 day day NN cord-266018-8bhnlsgy 163 8 2 2 CD cord-266018-8bhnlsgy 163 9 p.i p.i NNP cord-266018-8bhnlsgy 163 10 . . NNP cord-266018-8bhnlsgy 163 11 did do VBD cord-266018-8bhnlsgy 163 12 not not RB cord-266018-8bhnlsgy 163 13 express express VB cord-266018-8bhnlsgy 163 14 detectable detectable JJ cord-266018-8bhnlsgy 163 15 levels level NNS cord-266018-8bhnlsgy 163 16 of of IN cord-266018-8bhnlsgy 163 17 either either CC cord-266018-8bhnlsgy 163 18 CD8a CD8a NNP cord-266018-8bhnlsgy 163 19 or or CC cord-266018-8bhnlsgy 163 20 DEC205 DEC205 NNP cord-266018-8bhnlsgy 163 21 while while IN cord-266018-8bhnlsgy 163 22 expression expression NN cord-266018-8bhnlsgy 163 23 of of IN cord-266018-8bhnlsgy 163 24 both both CC cord-266018-8bhnlsgy 163 25 CD8a CD8a NNP cord-266018-8bhnlsgy 163 26 and and CC cord-266018-8bhnlsgy 163 27 DEC205 DEC205 NNP cord-266018-8bhnlsgy 163 28 was be VBD cord-266018-8bhnlsgy 163 29 readily readily RB cord-266018-8bhnlsgy 163 30 detectable detectable JJ cord-266018-8bhnlsgy 163 31 on on IN cord-266018-8bhnlsgy 163 32 CD11c CD11c NNP cord-266018-8bhnlsgy 163 33 + + CC cord-266018-8bhnlsgy 164 1 CD11b CD11b NNS cord-266018-8bhnlsgy 164 2 À à NN cord-266018-8bhnlsgy 164 3 cells cell NNS cord-266018-8bhnlsgy 164 4 present present JJ cord-266018-8bhnlsgy 164 5 within within IN cord-266018-8bhnlsgy 164 6 the the DT cord-266018-8bhnlsgy 164 7 brains brain NNS cord-266018-8bhnlsgy 164 8 of of IN cord-266018-8bhnlsgy 164 9 both both CC cord-266018-8bhnlsgy 164 10 CCL3 CCL3 NNP cord-266018-8bhnlsgy 164 11 + + CC cord-266018-8bhnlsgy 164 12 /+ /+ . cord-266018-8bhnlsgy 164 13 and and CC cord-266018-8bhnlsgy 164 14 CCL3 CCL3 NNP cord-266018-8bhnlsgy 164 15 À À NNP cord-266018-8bhnlsgy 164 16 / / SYM cord-266018-8bhnlsgy 164 17 À à NN cord-266018-8bhnlsgy 164 18 mice mouse NNS cord-266018-8bhnlsgy 164 19 at at IN cord-266018-8bhnlsgy 164 20 day day NN cord-266018-8bhnlsgy 164 21 2 2 CD cord-266018-8bhnlsgy 164 22 p.i p.i NNP cord-266018-8bhnlsgy 164 23 . . . cord-266018-8bhnlsgy 165 1 ( ( -LRB- cord-266018-8bhnlsgy 165 2 C C NNP cord-266018-8bhnlsgy 165 3 ) ) -RRB- cord-266018-8bhnlsgy 165 4 Total total JJ cord-266018-8bhnlsgy 165 5 numbers number NNS cord-266018-8bhnlsgy 165 6 of of IN cord-266018-8bhnlsgy 165 7 CD8a CD8a NNP cord-266018-8bhnlsgy 165 8 + + CC cord-266018-8bhnlsgy 165 9 and and CC cord-266018-8bhnlsgy 165 10 CD8a CD8a NNP cord-266018-8bhnlsgy 165 11 À À HYPH cord-266018-8bhnlsgy 165 12 DCs dc NNS cord-266018-8bhnlsgy 165 13 within within IN cord-266018-8bhnlsgy 165 14 the the DT cord-266018-8bhnlsgy 165 15 CLNs cln NNS cord-266018-8bhnlsgy 165 16 of of IN cord-266018-8bhnlsgy 165 17 MHV MHV NNP cord-266018-8bhnlsgy 165 18 or or CC cord-266018-8bhnlsgy 165 19 sham sham NN cord-266018-8bhnlsgy 165 20 - - HYPH cord-266018-8bhnlsgy 165 21 infected infected JJ cord-266018-8bhnlsgy 165 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 165 23 + + CC cord-266018-8bhnlsgy 166 1 /+ /+ . cord-266018-8bhnlsgy 167 1 and and CC cord-266018-8bhnlsgy 167 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 167 3 À À NNP cord-266018-8bhnlsgy 167 4 / / SYM cord-266018-8bhnlsgy 167 5 À à NN cord-266018-8bhnlsgy 167 6 mice mouse NNS cord-266018-8bhnlsgy 167 7 at at IN cord-266018-8bhnlsgy 167 8 days day NNS cord-266018-8bhnlsgy 167 9 2 2 CD cord-266018-8bhnlsgy 167 10 and and CC cord-266018-8bhnlsgy 168 1 5 5 CD cord-266018-8bhnlsgy 168 2 p.i p.i NNP cord-266018-8bhnlsgy 168 3 . . NNP cord-266018-8bhnlsgy 168 4 Data Data NNP cord-266018-8bhnlsgy 168 5 presented present VBD cord-266018-8bhnlsgy 168 6 represent represent VBP cord-266018-8bhnlsgy 168 7 an an DT cord-266018-8bhnlsgy 168 8 average average JJ cord-266018-8bhnlsgy 168 9 cell cell NN cord-266018-8bhnlsgy 168 10 number number NN cord-266018-8bhnlsgy 168 11 derived derive VBN cord-266018-8bhnlsgy 168 12 from from IN cord-266018-8bhnlsgy 168 13 two two CD cord-266018-8bhnlsgy 168 14 separate separate JJ cord-266018-8bhnlsgy 168 15 experiments experiment NNS cord-266018-8bhnlsgy 168 16 with with IN cord-266018-8bhnlsgy 168 17 a a DT cord-266018-8bhnlsgy 168 18 minimum minimum NN cord-266018-8bhnlsgy 168 19 of of IN cord-266018-8bhnlsgy 168 20 10 10 CD cord-266018-8bhnlsgy 168 21 mice mouse NNS cord-266018-8bhnlsgy 168 22 analyzed analyze VBN cord-266018-8bhnlsgy 168 23 per per IN cord-266018-8bhnlsgy 168 24 experimental experimental JJ cord-266018-8bhnlsgy 168 25 group group NN cord-266018-8bhnlsgy 168 26 . . . cord-266018-8bhnlsgy 169 1 ( ( -LRB- cord-266018-8bhnlsgy 169 2 D D NNP cord-266018-8bhnlsgy 169 3 ) ) -RRB- cord-266018-8bhnlsgy 169 4 CD8a CD8a NNP cord-266018-8bhnlsgy 169 5 À à NN cord-266018-8bhnlsgy 169 6 cells cell NNS cord-266018-8bhnlsgy 169 7 obtained obtain VBN cord-266018-8bhnlsgy 169 8 from from IN cord-266018-8bhnlsgy 169 9 MHV mhv NN cord-266018-8bhnlsgy 169 10 - - HYPH cord-266018-8bhnlsgy 169 11 infected infect VBN cord-266018-8bhnlsgy 169 12 ( ( -LRB- cord-266018-8bhnlsgy 169 13 day day NN cord-266018-8bhnlsgy 169 14 2 2 CD cord-266018-8bhnlsgy 169 15 p.i p.i NNP cord-266018-8bhnlsgy 169 16 . . NNP cord-266018-8bhnlsgy 169 17 ) ) -RRB- cord-266018-8bhnlsgy 169 18 or or CC cord-266018-8bhnlsgy 169 19 sham sham JJ cord-266018-8bhnlsgy 169 20 mice mouse NNS cord-266018-8bhnlsgy 169 21 were be VBD cord-266018-8bhnlsgy 169 22 gated gate VBN cord-266018-8bhnlsgy 169 23 and and CC cord-266018-8bhnlsgy 169 24 the the DT cord-266018-8bhnlsgy 169 25 level level NN cord-266018-8bhnlsgy 169 26 of of IN cord-266018-8bhnlsgy 169 27 CD80 CD80 NNP cord-266018-8bhnlsgy 169 28 , , , cord-266018-8bhnlsgy 169 29 CD86 CD86 NNP cord-266018-8bhnlsgy 169 30 , , , cord-266018-8bhnlsgy 169 31 and and CC cord-266018-8bhnlsgy 169 32 CD40 cd40 JJ cord-266018-8bhnlsgy 169 33 expression expression NN cord-266018-8bhnlsgy 169 34 was be VBD cord-266018-8bhnlsgy 169 35 determined determine VBN cord-266018-8bhnlsgy 169 36 by by IN cord-266018-8bhnlsgy 169 37 flow flow NN cord-266018-8bhnlsgy 169 38 cytometry cytometry NN cord-266018-8bhnlsgy 169 39 . . . cord-266018-8bhnlsgy 170 1 The the DT cord-266018-8bhnlsgy 170 2 MFI MFI NNP cord-266018-8bhnlsgy 170 3 for for IN cord-266018-8bhnlsgy 170 4 cells cell NNS cord-266018-8bhnlsgy 170 5 obtained obtain VBN cord-266018-8bhnlsgy 170 6 from from IN cord-266018-8bhnlsgy 170 7 either either CC cord-266018-8bhnlsgy 170 8 CCL3 CCL3 NNP cord-266018-8bhnlsgy 170 9 + + CD cord-266018-8bhnlsgy 171 1 /+ /+ NFP cord-266018-8bhnlsgy 171 2 or or CC cord-266018-8bhnlsgy 172 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 172 2 À à NN cord-266018-8bhnlsgy 172 3 / / SYM cord-266018-8bhnlsgy 172 4 À à NN cord-266018-8bhnlsgy 172 5 mice mouse NNS cord-266018-8bhnlsgy 172 6 is be VBZ cord-266018-8bhnlsgy 172 7 indicated indicate VBN cord-266018-8bhnlsgy 172 8 . . . cord-266018-8bhnlsgy 173 1 ( ( -LRB- cord-266018-8bhnlsgy 173 2 E e NN cord-266018-8bhnlsgy 173 3 ) ) -RRB- cord-266018-8bhnlsgy 173 4 CD8a CD8a NNP cord-266018-8bhnlsgy 173 5 À à NN cord-266018-8bhnlsgy 173 6 cells cell NNS cord-266018-8bhnlsgy 173 7 obtained obtain VBN cord-266018-8bhnlsgy 173 8 from from IN cord-266018-8bhnlsgy 173 9 MHV mhv NN cord-266018-8bhnlsgy 173 10 - - HYPH cord-266018-8bhnlsgy 173 11 infected infect VBN cord-266018-8bhnlsgy 173 12 or or CC cord-266018-8bhnlsgy 173 13 sham sham JJ cord-266018-8bhnlsgy 173 14 mice mouse NNS cord-266018-8bhnlsgy 173 15 were be VBD cord-266018-8bhnlsgy 173 16 evaluated evaluate VBN cord-266018-8bhnlsgy 173 17 for for IN cord-266018-8bhnlsgy 173 18 expression expression NN cord-266018-8bhnlsgy 173 19 of of IN cord-266018-8bhnlsgy 173 20 MHC MHC NNP cord-266018-8bhnlsgy 173 21 I I NNP cord-266018-8bhnlsgy 173 22 and and CC cord-266018-8bhnlsgy 173 23 II II NNP cord-266018-8bhnlsgy 173 24 . . . cord-266018-8bhnlsgy 174 1 The the DT cord-266018-8bhnlsgy 174 2 MFI MFI NNP cord-266018-8bhnlsgy 174 3 for for IN cord-266018-8bhnlsgy 174 4 staining staining NN cord-266018-8bhnlsgy 174 5 of of IN cord-266018-8bhnlsgy 174 6 either either DT cord-266018-8bhnlsgy 174 7 MHC MHC NNP cord-266018-8bhnlsgy 174 8 I I NNP cord-266018-8bhnlsgy 174 9 or or CC cord-266018-8bhnlsgy 174 10 II II NNP cord-266018-8bhnlsgy 174 11 is be VBZ cord-266018-8bhnlsgy 174 12 indicated indicate VBN cord-266018-8bhnlsgy 174 13 in in IN cord-266018-8bhnlsgy 174 14 the the DT cord-266018-8bhnlsgy 174 15 histogram histogram NN cord-266018-8bhnlsgy 174 16 . . . cord-266018-8bhnlsgy 175 1 Flow flow NN cord-266018-8bhnlsgy 175 2 data datum NNS cord-266018-8bhnlsgy 175 3 shown show VBN cord-266018-8bhnlsgy 175 4 in in IN cord-266018-8bhnlsgy 175 5 panels panel NNS cord-266018-8bhnlsgy 175 6 A A NNP cord-266018-8bhnlsgy 175 7 , , , cord-266018-8bhnlsgy 175 8 B b NN cord-266018-8bhnlsgy 175 9 , , , cord-266018-8bhnlsgy 175 10 D D NNP cord-266018-8bhnlsgy 175 11 , , , cord-266018-8bhnlsgy 175 12 and and CC cord-266018-8bhnlsgy 175 13 E E NNP cord-266018-8bhnlsgy 175 14 are be VBP cord-266018-8bhnlsgy 175 15 representative representative JJ cord-266018-8bhnlsgy 175 16 of of IN cord-266018-8bhnlsgy 175 17 two two CD cord-266018-8bhnlsgy 175 18 separate separate JJ cord-266018-8bhnlsgy 175 19 experiments experiment NNS cord-266018-8bhnlsgy 175 20 with with IN cord-266018-8bhnlsgy 175 21 a a DT cord-266018-8bhnlsgy 175 22 total total NN cord-266018-8bhnlsgy 175 23 of of IN cord-266018-8bhnlsgy 175 24 10 10 CD cord-266018-8bhnlsgy 175 25 mice mouse NNS cord-266018-8bhnlsgy 175 26 for for IN cord-266018-8bhnlsgy 175 27 each each DT cord-266018-8bhnlsgy 175 28 experimental experimental JJ cord-266018-8bhnlsgy 175 29 condition condition NN cord-266018-8bhnlsgy 175 30 . . . cord-266018-8bhnlsgy 176 1 The the DT cord-266018-8bhnlsgy 176 2 activation activation NN cord-266018-8bhnlsgy 176 3 of of IN cord-266018-8bhnlsgy 176 4 DC DC NNP cord-266018-8bhnlsgy 176 5 and and CC cord-266018-8bhnlsgy 176 6 their -PRON- PRP$ cord-266018-8bhnlsgy 176 7 mobilization mobilization NN cord-266018-8bhnlsgy 176 8 to to IN cord-266018-8bhnlsgy 176 9 secondary secondary JJ cord-266018-8bhnlsgy 176 10 lymphoid lymphoid JJ cord-266018-8bhnlsgy 176 11 organs organ NNS cord-266018-8bhnlsgy 176 12 is be VBZ cord-266018-8bhnlsgy 176 13 thought think VBN cord-266018-8bhnlsgy 176 14 to to TO cord-266018-8bhnlsgy 176 15 be be VB cord-266018-8bhnlsgy 176 16 a a DT cord-266018-8bhnlsgy 176 17 key key JJ cord-266018-8bhnlsgy 176 18 step step NN cord-266018-8bhnlsgy 176 19 in in IN cord-266018-8bhnlsgy 176 20 the the DT cord-266018-8bhnlsgy 176 21 initiation initiation NN cord-266018-8bhnlsgy 176 22 of of IN cord-266018-8bhnlsgy 176 23 an an DT cord-266018-8bhnlsgy 176 24 adaptive adaptive JJ cord-266018-8bhnlsgy 176 25 immune immune JJ cord-266018-8bhnlsgy 176 26 response response NN cord-266018-8bhnlsgy 176 27 ( ( -LRB- cord-266018-8bhnlsgy 176 28 Banchereau Banchereau NNP cord-266018-8bhnlsgy 176 29 and and CC cord-266018-8bhnlsgy 176 30 Steinman Steinman NNP cord-266018-8bhnlsgy 176 31 , , , cord-266018-8bhnlsgy 176 32 1998 1998 CD cord-266018-8bhnlsgy 176 33 ) ) -RRB- cord-266018-8bhnlsgy 176 34 . . . cord-266018-8bhnlsgy 177 1 Recent recent JJ cord-266018-8bhnlsgy 177 2 studies study NNS cord-266018-8bhnlsgy 177 3 have have VBP cord-266018-8bhnlsgy 177 4 indicated indicate VBN cord-266018-8bhnlsgy 177 5 that that IN cord-266018-8bhnlsgy 177 6 following follow VBG cord-266018-8bhnlsgy 177 7 infection infection NN cord-266018-8bhnlsgy 177 8 of of IN cord-266018-8bhnlsgy 177 9 the the DT cord-266018-8bhnlsgy 177 10 CNS CNS NNP cord-266018-8bhnlsgy 177 11 with with IN cord-266018-8bhnlsgy 177 12 Toxoplasma toxoplasma NN cord-266018-8bhnlsgy 177 13 gondii gondii NN cord-266018-8bhnlsgy 177 14 , , , cord-266018-8bhnlsgy 177 15 CD11c CD11c NNP cord-266018-8bhnlsgy 177 16 + + CC cord-266018-8bhnlsgy 177 17 cells cell NNS cord-266018-8bhnlsgy 177 18 are be VBP cord-266018-8bhnlsgy 177 19 present present JJ cord-266018-8bhnlsgy 177 20 within within IN cord-266018-8bhnlsgy 177 21 the the DT cord-266018-8bhnlsgy 177 22 brain brain NN cord-266018-8bhnlsgy 177 23 and and CC cord-266018-8bhnlsgy 177 24 these these DT cord-266018-8bhnlsgy 177 25 cells cell NNS cord-266018-8bhnlsgy 177 26 were be VBD cord-266018-8bhnlsgy 177 27 able able JJ cord-266018-8bhnlsgy 177 28 to to TO cord-266018-8bhnlsgy 177 29 stimulate stimulate VB cord-266018-8bhnlsgy 177 30 the the DT cord-266018-8bhnlsgy 177 31 proliferation proliferation NN cord-266018-8bhnlsgy 177 32 of of IN cord-266018-8bhnlsgy 177 33 naRve naRve NNP cord-266018-8bhnlsgy 177 34 T T NNP cord-266018-8bhnlsgy 177 35 cells cell NNS cord-266018-8bhnlsgy 177 36 ( ( -LRB- cord-266018-8bhnlsgy 177 37 Fisher Fisher NNP cord-266018-8bhnlsgy 177 38 and and CC cord-266018-8bhnlsgy 177 39 Reichmann Reichmann NNP cord-266018-8bhnlsgy 177 40 , , , cord-266018-8bhnlsgy 177 41 2001 2001 CD cord-266018-8bhnlsgy 177 42 ) ) -RRB- cord-266018-8bhnlsgy 177 43 . . . cord-266018-8bhnlsgy 178 1 Similarly similarly RB cord-266018-8bhnlsgy 178 2 , , , cord-266018-8bhnlsgy 178 3 our -PRON- PRP$ cord-266018-8bhnlsgy 178 4 results result NNS cord-266018-8bhnlsgy 178 5 also also RB cord-266018-8bhnlsgy 178 6 indicate indicate VBP cord-266018-8bhnlsgy 178 7 an an DT cord-266018-8bhnlsgy 178 8 increase increase NN cord-266018-8bhnlsgy 178 9 in in IN cord-266018-8bhnlsgy 178 10 CD11c CD11c NNP cord-266018-8bhnlsgy 178 11 + + CC cord-266018-8bhnlsgy 178 12 cells cell NNS cord-266018-8bhnlsgy 178 13 within within IN cord-266018-8bhnlsgy 178 14 the the DT cord-266018-8bhnlsgy 178 15 CNS CNS NNP cord-266018-8bhnlsgy 178 16 following follow VBG cord-266018-8bhnlsgy 178 17 viral viral JJ cord-266018-8bhnlsgy 178 18 infection infection NN cord-266018-8bhnlsgy 178 19 , , , cord-266018-8bhnlsgy 178 20 suggesting suggest VBG cord-266018-8bhnlsgy 178 21 that that IN cord-266018-8bhnlsgy 178 22 these these DT cord-266018-8bhnlsgy 178 23 cells cell NNS cord-266018-8bhnlsgy 178 24 are be VBP cord-266018-8bhnlsgy 178 25 likely likely RB cord-266018-8bhnlsgy 178 26 critical critical JJ cord-266018-8bhnlsgy 178 27 for for IN cord-266018-8bhnlsgy 178 28 successful successful JJ cord-266018-8bhnlsgy 178 29 T t NN cord-266018-8bhnlsgy 178 30 cell cell NN cord-266018-8bhnlsgy 178 31 priming priming NN cord-266018-8bhnlsgy 178 32 following follow VBG cord-266018-8bhnlsgy 178 33 migration migration NN cord-266018-8bhnlsgy 178 34 to to IN cord-266018-8bhnlsgy 178 35 draining drain VBG cord-266018-8bhnlsgy 178 36 lymph lymph NN cord-266018-8bhnlsgy 178 37 nodes node NNS cord-266018-8bhnlsgy 178 38 . . . cord-266018-8bhnlsgy 179 1 Whether whether IN cord-266018-8bhnlsgy 179 2 these these DT cord-266018-8bhnlsgy 179 3 cells cell NNS cord-266018-8bhnlsgy 179 4 are be VBP cord-266018-8bhnlsgy 179 5 present present JJ cord-266018-8bhnlsgy 179 6 within within IN cord-266018-8bhnlsgy 179 7 the the DT cord-266018-8bhnlsgy 179 8 CNS CNS NNP cord-266018-8bhnlsgy 179 9 by by IN cord-266018-8bhnlsgy 179 10 differentiation differentiation NN cord-266018-8bhnlsgy 179 11 of of IN cord-266018-8bhnlsgy 179 12 local local JJ cord-266018-8bhnlsgy 179 13 antigen antigen NN cord-266018-8bhnlsgy 179 14 presenting present VBG cord-266018-8bhnlsgy 179 15 cells cell NNS cord-266018-8bhnlsgy 179 16 , , , cord-266018-8bhnlsgy 179 17 or or CC cord-266018-8bhnlsgy 179 18 through through IN cord-266018-8bhnlsgy 179 19 migration migration NN cord-266018-8bhnlsgy 179 20 of of IN cord-266018-8bhnlsgy 179 21 immature immature JJ cord-266018-8bhnlsgy 179 22 DC DC NNP cord-266018-8bhnlsgy 179 23 has have VBZ cord-266018-8bhnlsgy 179 24 not not RB cord-266018-8bhnlsgy 179 25 been be VBN cord-266018-8bhnlsgy 179 26 determined determine VBN cord-266018-8bhnlsgy 179 27 and and CC cord-266018-8bhnlsgy 179 28 is be VBZ cord-266018-8bhnlsgy 179 29 currently currently RB cord-266018-8bhnlsgy 179 30 under under IN cord-266018-8bhnlsgy 179 31 investigation investigation NN cord-266018-8bhnlsgy 179 32 . . . cord-266018-8bhnlsgy 180 1 Regardless regardless RB cord-266018-8bhnlsgy 180 2 , , , cord-266018-8bhnlsgy 180 3 our -PRON- PRP$ cord-266018-8bhnlsgy 180 4 data datum NNS cord-266018-8bhnlsgy 180 5 imply imply VBP cord-266018-8bhnlsgy 180 6 that that IN cord-266018-8bhnlsgy 180 7 CCL3 CCL3 NNP cord-266018-8bhnlsgy 180 8 expression expression NN cord-266018-8bhnlsgy 180 9 and and CC cord-266018-8bhnlsgy 180 10 signaling signaling NN cord-266018-8bhnlsgy 180 11 contributes contribute VBZ cord-266018-8bhnlsgy 180 12 to to IN cord-266018-8bhnlsgy 180 13 the the DT cord-266018-8bhnlsgy 180 14 migration migration NN cord-266018-8bhnlsgy 180 15 of of IN cord-266018-8bhnlsgy 180 16 CD8a CD8a NNP cord-266018-8bhnlsgy 180 17 À À HYPH cord-266018-8bhnlsgy 180 18 CD11c CD11c NNP cord-266018-8bhnlsgy 180 19 + + IN cord-266018-8bhnlsgy 180 20 cells cell NNS cord-266018-8bhnlsgy 180 21 to to IN cord-266018-8bhnlsgy 180 22 secondary secondary JJ cord-266018-8bhnlsgy 180 23 lymphoid lymphoid JJ cord-266018-8bhnlsgy 180 24 tissue tissue NN cord-266018-8bhnlsgy 180 25 where where WRB cord-266018-8bhnlsgy 180 26 they -PRON- PRP cord-266018-8bhnlsgy 180 27 participate participate VBP cord-266018-8bhnlsgy 180 28 in in IN cord-266018-8bhnlsgy 180 29 priming priming NN cord-266018-8bhnlsgy 180 30 of of IN cord-266018-8bhnlsgy 180 31 T t NN cord-266018-8bhnlsgy 180 32 cells cell NNS cord-266018-8bhnlsgy 180 33 . . . cord-266018-8bhnlsgy 181 1 In in IN cord-266018-8bhnlsgy 181 2 support support NN cord-266018-8bhnlsgy 181 3 of of IN cord-266018-8bhnlsgy 181 4 this this DT cord-266018-8bhnlsgy 181 5 , , , cord-266018-8bhnlsgy 181 6 we -PRON- PRP cord-266018-8bhnlsgy 181 7 have have VBP cord-266018-8bhnlsgy 181 8 shown show VBN cord-266018-8bhnlsgy 181 9 that that IN cord-266018-8bhnlsgy 181 10 CCL3 CCL3 NNP cord-266018-8bhnlsgy 181 11 is be VBZ cord-266018-8bhnlsgy 181 12 important important JJ cord-266018-8bhnlsgy 181 13 in in IN cord-266018-8bhnlsgy 181 14 arming arm VBG cord-266018-8bhnlsgy 181 15 these these DT cord-266018-8bhnlsgy 181 16 cells cell NNS cord-266018-8bhnlsgy 181 17 with with IN cord-266018-8bhnlsgy 181 18 the the DT cord-266018-8bhnlsgy 181 19 capability capability NN cord-266018-8bhnlsgy 181 20 to to TO cord-266018-8bhnlsgy 181 21 optimally optimally RB cord-266018-8bhnlsgy 181 22 stimulate stimulate VB cord-266018-8bhnlsgy 181 23 antigen antigen NN cord-266018-8bhnlsgy 181 24 - - HYPH cord-266018-8bhnlsgy 181 25 specific specific JJ cord-266018-8bhnlsgy 181 26 T t NN cord-266018-8bhnlsgy 181 27 cells cell NNS cord-266018-8bhnlsgy 181 28 with with IN cord-266018-8bhnlsgy 181 29 the the DT cord-266018-8bhnlsgy 181 30 ability ability NN cord-266018-8bhnlsgy 181 31 to to TO cord-266018-8bhnlsgy 181 32 fully fully RB cord-266018-8bhnlsgy 181 33 differentiate differentiate VB cord-266018-8bhnlsgy 181 34 into into IN cord-266018-8bhnlsgy 181 35 effector effector NN cord-266018-8bhnlsgy 181 36 cells cell NNS cord-266018-8bhnlsgy 181 37 ( ( -LRB- cord-266018-8bhnlsgy 182 1 Trifilo Trifilo NNP cord-266018-8bhnlsgy 182 2 et et FW cord-266018-8bhnlsgy 182 3 al al NNP cord-266018-8bhnlsgy 182 4 . . NNP cord-266018-8bhnlsgy 182 5 , , , cord-266018-8bhnlsgy 182 6 2003 2003 CD cord-266018-8bhnlsgy 182 7 ) ) -RRB- cord-266018-8bhnlsgy 182 8 . . . cord-266018-8bhnlsgy 183 1 The the DT cord-266018-8bhnlsgy 183 2 data datum NNS cord-266018-8bhnlsgy 183 3 presented present VBN cord-266018-8bhnlsgy 183 4 in in IN cord-266018-8bhnlsgy 183 5 this this DT cord-266018-8bhnlsgy 183 6 study study NN cord-266018-8bhnlsgy 183 7 support support NN cord-266018-8bhnlsgy 183 8 and and CC cord-266018-8bhnlsgy 183 9 extend extend VB cord-266018-8bhnlsgy 183 10 these these DT cord-266018-8bhnlsgy 183 11 observations observation NNS cord-266018-8bhnlsgy 183 12 and and CC cord-266018-8bhnlsgy 183 13 indicate indicate VBP cord-266018-8bhnlsgy 183 14 that that IN cord-266018-8bhnlsgy 183 15 these these DT cord-266018-8bhnlsgy 183 16 results result NNS cord-266018-8bhnlsgy 183 17 may may MD cord-266018-8bhnlsgy 183 18 be be VB cord-266018-8bhnlsgy 183 19 the the DT cord-266018-8bhnlsgy 183 20 result result NN cord-266018-8bhnlsgy 183 21 of of IN cord-266018-8bhnlsgy 183 22 a a DT cord-266018-8bhnlsgy 183 23 combination combination NN cord-266018-8bhnlsgy 183 24 of of IN cord-266018-8bhnlsgy 183 25 diminished diminished JJ cord-266018-8bhnlsgy 183 26 expression expression NN cord-266018-8bhnlsgy 183 27 of of IN cord-266018-8bhnlsgy 183 28 both both CC cord-266018-8bhnlsgy 183 29 MHC MHC NNP cord-266018-8bhnlsgy 183 30 class class NN cord-266018-8bhnlsgy 183 31 I i NN cord-266018-8bhnlsgy 183 32 and and CC cord-266018-8bhnlsgy 183 33 II II NNP cord-266018-8bhnlsgy 183 34 , , , cord-266018-8bhnlsgy 183 35 reduced reduced JJ cord-266018-8bhnlsgy 183 36 expression expression NN cord-266018-8bhnlsgy 183 37 of of IN cord-266018-8bhnlsgy 183 38 CD40 CD40 NNP cord-266018-8bhnlsgy 183 39 , , , cord-266018-8bhnlsgy 183 40 as as RB cord-266018-8bhnlsgy 183 41 well well RB cord-266018-8bhnlsgy 183 42 as as IN cord-266018-8bhnlsgy 183 43 a a DT cord-266018-8bhnlsgy 183 44 shift shift NN cord-266018-8bhnlsgy 183 45 in in IN cord-266018-8bhnlsgy 183 46 cytokine cytokine NN cord-266018-8bhnlsgy 183 47 production production NN cord-266018-8bhnlsgy 183 48 by by IN cord-266018-8bhnlsgy 183 49 CD8a CD8a NNP cord-266018-8bhnlsgy 183 50 À à NN cord-266018-8bhnlsgy 183 51 cells cell NNS cord-266018-8bhnlsgy 183 52 . . . cord-266018-8bhnlsgy 184 1 Indeed indeed RB cord-266018-8bhnlsgy 184 2 , , , cord-266018-8bhnlsgy 184 3 CD40L cd40l NN cord-266018-8bhnlsgy 184 4 : : : cord-266018-8bhnlsgy 184 5 CD40 CD40 NNP cord-266018-8bhnlsgy 184 6 mediated mediate VBD cord-266018-8bhnlsgy 184 7 interactions interaction NNS cord-266018-8bhnlsgy 184 8 between between IN cord-266018-8bhnlsgy 184 9 T T NNP cord-266018-8bhnlsgy 184 10 cells cell NNS cord-266018-8bhnlsgy 184 11 and and CC cord-266018-8bhnlsgy 184 12 APC APC NNP cord-266018-8bhnlsgy 184 13 can can MD cord-266018-8bhnlsgy 184 14 enhance enhance VB cord-266018-8bhnlsgy 184 15 IL-12 IL-12 NNP cord-266018-8bhnlsgy 184 16 production production NN cord-266018-8bhnlsgy 184 17 by by IN cord-266018-8bhnlsgy 184 18 DC DC NNP cord-266018-8bhnlsgy 184 19 and and CC cord-266018-8bhnlsgy 184 20 blockade blockade NN cord-266018-8bhnlsgy 184 21 of of IN cord-266018-8bhnlsgy 184 22 this this DT cord-266018-8bhnlsgy 184 23 interaction interaction NN cord-266018-8bhnlsgy 184 24 has have VBZ cord-266018-8bhnlsgy 184 25 been be VBN cord-266018-8bhnlsgy 184 26 shown show VBN cord-266018-8bhnlsgy 184 27 to to TO cord-266018-8bhnlsgy 184 28 result result VB cord-266018-8bhnlsgy 184 29 in in IN cord-266018-8bhnlsgy 184 30 reduced reduce VBN cord-266018-8bhnlsgy 184 31 autoimmunity autoimmunity NN cord-266018-8bhnlsgy 184 32 by by IN cord-266018-8bhnlsgy 184 33 down down IN cord-266018-8bhnlsgy 184 34 - - HYPH cord-266018-8bhnlsgy 184 35 regulating regulate VBG cord-266018-8bhnlsgy 184 36 Th1 th1 NN cord-266018-8bhnlsgy 184 37 differentiation differentiation NN cord-266018-8bhnlsgy 185 1 ( ( -LRB- cord-266018-8bhnlsgy 185 2 Cella Cella NNP cord-266018-8bhnlsgy 185 3 et et FW cord-266018-8bhnlsgy 185 4 al al NNP cord-266018-8bhnlsgy 185 5 . . NNP cord-266018-8bhnlsgy 185 6 , , , cord-266018-8bhnlsgy 185 7 1996 1996 CD cord-266018-8bhnlsgy 186 1 ; ; : cord-266018-8bhnlsgy 186 2 Macatonia Macatonia NNP cord-266018-8bhnlsgy 186 3 et et NNP cord-266018-8bhnlsgy 186 4 al al NNP cord-266018-8bhnlsgy 186 5 . . NNP cord-266018-8bhnlsgy 186 6 , , , cord-266018-8bhnlsgy 186 7 1995 1995 CD cord-266018-8bhnlsgy 186 8 ) ) -RRB- cord-266018-8bhnlsgy 186 9 . . . cord-266018-8bhnlsgy 187 1 Accumulating accumulate VBG cord-266018-8bhnlsgy 187 2 evidence evidence NN cord-266018-8bhnlsgy 187 3 indicates indicate VBZ cord-266018-8bhnlsgy 187 4 that that IN cord-266018-8bhnlsgy 187 5 in in IN cord-266018-8bhnlsgy 187 6 addition addition NN cord-266018-8bhnlsgy 187 7 to to IN cord-266018-8bhnlsgy 187 8 driving drive VBG cord-266018-8bhnlsgy 187 9 virus virus NN cord-266018-8bhnlsgy 187 10 - - HYPH cord-266018-8bhnlsgy 187 11 specific specific JJ cord-266018-8bhnlsgy 187 12 T t NN cord-266018-8bhnlsgy 187 13 cell cell NN cord-266018-8bhnlsgy 187 14 proliferation proliferation NN cord-266018-8bhnlsgy 187 15 , , , cord-266018-8bhnlsgy 187 16 the the DT cord-266018-8bhnlsgy 187 17 activation activation NN cord-266018-8bhnlsgy 187 18 state state NN cord-266018-8bhnlsgy 187 19 of of IN cord-266018-8bhnlsgy 187 20 DCs DCs NNP cord-266018-8bhnlsgy 187 21 can can MD cord-266018-8bhnlsgy 187 22 also also RB cord-266018-8bhnlsgy 187 23 directly directly RB cord-266018-8bhnlsgy 187 24 influence influence VB cord-266018-8bhnlsgy 187 25 the the DT cord-266018-8bhnlsgy 187 26 effector effector JJ cord-266018-8bhnlsgy 187 27 function function NN cord-266018-8bhnlsgy 187 28 of of IN cord-266018-8bhnlsgy 187 29 T t NN cord-266018-8bhnlsgy 187 30 cells cell NNS cord-266018-8bhnlsgy 187 31 through through IN cord-266018-8bhnlsgy 187 32 the the DT cord-266018-8bhnlsgy 187 33 secretion secretion NN cord-266018-8bhnlsgy 187 34 of of IN cord-266018-8bhnlsgy 187 35 proinflammatory proinflammatory JJ cord-266018-8bhnlsgy 187 36 cytokines cytokine NNS cord-266018-8bhnlsgy 187 37 ( ( -LRB- cord-266018-8bhnlsgy 187 38 Fischer Fischer NNP cord-266018-8bhnlsgy 187 39 et et NNP cord-266018-8bhnlsgy 187 40 al al NNP cord-266018-8bhnlsgy 187 41 . . NNP cord-266018-8bhnlsgy 187 42 , , , cord-266018-8bhnlsgy 187 43 2000 2000 CD cord-266018-8bhnlsgy 187 44 ; ; : cord-266018-8bhnlsgy 187 45 Maldonado Maldonado NNP cord-266018-8bhnlsgy 187 46 - - HYPH cord-266018-8bhnlsgy 187 47 Lopez Lopez NNP cord-266018-8bhnlsgy 187 48 et et NNP cord-266018-8bhnlsgy 187 49 al al NNP cord-266018-8bhnlsgy 187 50 . . NNP cord-266018-8bhnlsgy 187 51 , , , cord-266018-8bhnlsgy 187 52 1999 1999 CD cord-266018-8bhnlsgy 188 1 Pulendran Pulendran NNP cord-266018-8bhnlsgy 188 2 et et NNP cord-266018-8bhnlsgy 188 3 al al NNP cord-266018-8bhnlsgy 188 4 . . NNP cord-266018-8bhnlsgy 188 5 , , , cord-266018-8bhnlsgy 188 6 1999 1999 CD cord-266018-8bhnlsgy 188 7 ) ) -RRB- cord-266018-8bhnlsgy 188 8 . . . cord-266018-8bhnlsgy 189 1 For for IN cord-266018-8bhnlsgy 189 2 example example NN cord-266018-8bhnlsgy 189 3 , , , cord-266018-8bhnlsgy 189 4 following follow VBG cord-266018-8bhnlsgy 189 5 several several JJ cord-266018-8bhnlsgy 189 6 viral viral JJ cord-266018-8bhnlsgy 189 7 and and CC cord-266018-8bhnlsgy 189 8 bacterial bacterial JJ cord-266018-8bhnlsgy 189 9 infections infection NNS cord-266018-8bhnlsgy 189 10 , , , cord-266018-8bhnlsgy 189 11 CD8a CD8a NNP cord-266018-8bhnlsgy 189 12 + + CC cord-266018-8bhnlsgy 189 13 DCs dc NNS cord-266018-8bhnlsgy 189 14 have have VBP cord-266018-8bhnlsgy 189 15 been be VBN cord-266018-8bhnlsgy 189 16 shown show VBN cord-266018-8bhnlsgy 189 17 to to TO cord-266018-8bhnlsgy 189 18 be be VB cord-266018-8bhnlsgy 189 19 able able JJ cord-266018-8bhnlsgy 189 20 to to TO cord-266018-8bhnlsgy 189 21 secrete secrete VB cord-266018-8bhnlsgy 189 22 large large JJ cord-266018-8bhnlsgy 189 23 amounts amount NNS cord-266018-8bhnlsgy 189 24 of of IN cord-266018-8bhnlsgy 189 25 the the DT cord-266018-8bhnlsgy 189 26 proinflammatory proinflammatory JJ cord-266018-8bhnlsgy 189 27 cytokine cytokine NN cord-266018-8bhnlsgy 189 28 IL-12 IL-12 NNP cord-266018-8bhnlsgy 189 29 both both CC cord-266018-8bhnlsgy 189 30 in in IN cord-266018-8bhnlsgy 189 31 vitro vitro FW cord-266018-8bhnlsgy 189 32 and and CC cord-266018-8bhnlsgy 189 33 in in IN cord-266018-8bhnlsgy 189 34 vivo vivo NN cord-266018-8bhnlsgy 190 1 and and CC cord-266018-8bhnlsgy 190 2 this this DT cord-266018-8bhnlsgy 190 3 results result VBZ cord-266018-8bhnlsgy 190 4 in in IN cord-266018-8bhnlsgy 190 5 a a DT cord-266018-8bhnlsgy 190 6 preferential preferential JJ cord-266018-8bhnlsgy 190 7 expression expression NN cord-266018-8bhnlsgy 190 8 of of IN cord-266018-8bhnlsgy 190 9 Th1-associated Th1-associated NNP cord-266018-8bhnlsgy 190 10 cytokines cytokine NNS cord-266018-8bhnlsgy 190 11 , , , cord-266018-8bhnlsgy 190 12 such such JJ cord-266018-8bhnlsgy 190 13 as as IN cord-266018-8bhnlsgy 190 14 IFN IFN NNP cord-266018-8bhnlsgy 190 15 - - HYPH cord-266018-8bhnlsgy 190 16 g g NNP cord-266018-8bhnlsgy 190 17 by by IN cord-266018-8bhnlsgy 190 18 responding respond VBG cord-266018-8bhnlsgy 190 19 T T NNP cord-266018-8bhnlsgy 190 20 cells cell NNS cord-266018-8bhnlsgy 191 1 ( ( -LRB- cord-266018-8bhnlsgy 191 2 Aliberti Aliberti NNP cord-266018-8bhnlsgy 191 3 et et NNP cord-266018-8bhnlsgy 191 4 al al NNP cord-266018-8bhnlsgy 191 5 . . NNP cord-266018-8bhnlsgy 191 6 , , , cord-266018-8bhnlsgy 191 7 2000 2000 CD cord-266018-8bhnlsgy 191 8 ; ; : cord-266018-8bhnlsgy 191 9 Maldonado Maldonado NNP cord-266018-8bhnlsgy 191 10 - - HYPH cord-266018-8bhnlsgy 191 11 Lopez Lopez NNP cord-266018-8bhnlsgy 191 12 et et NNP cord-266018-8bhnlsgy 191 13 al al NNP cord-266018-8bhnlsgy 191 14 . . NNP cord-266018-8bhnlsgy 191 15 , , , cord-266018-8bhnlsgy 191 16 1999 1999 CD cord-266018-8bhnlsgy 191 17 Pulendran Pulendran NNP cord-266018-8bhnlsgy 191 18 et et NNP cord-266018-8bhnlsgy 191 19 al al NNP cord-266018-8bhnlsgy 191 20 . . NNP cord-266018-8bhnlsgy 191 21 , , , cord-266018-8bhnlsgy 191 22 1999 1999 CD cord-266018-8bhnlsgy 191 23 ; ; : cord-266018-8bhnlsgy 191 24 Reis Reis NNP cord-266018-8bhnlsgy 191 25 e e NN cord-266018-8bhnlsgy 191 26 Sousa Sousa NNP cord-266018-8bhnlsgy 191 27 et et NNP cord-266018-8bhnlsgy 191 28 al al NNP cord-266018-8bhnlsgy 191 29 . . NNP cord-266018-8bhnlsgy 191 30 , , , cord-266018-8bhnlsgy 191 31 1997 1997 CD cord-266018-8bhnlsgy 191 32 ) ) -RRB- cord-266018-8bhnlsgy 191 33 . . . cord-266018-8bhnlsgy 192 1 Until until IN cord-266018-8bhnlsgy 192 2 recently recently RB cord-266018-8bhnlsgy 192 3 , , , cord-266018-8bhnlsgy 192 4 the the DT cord-266018-8bhnlsgy 192 5 prevailing prevail VBG cord-266018-8bhnlsgy 192 6 thought thought NN cord-266018-8bhnlsgy 192 7 was be VBD cord-266018-8bhnlsgy 192 8 that that IN cord-266018-8bhnlsgy 192 9 CD8a CD8a NNP cord-266018-8bhnlsgy 192 10 + + CC cord-266018-8bhnlsgy 192 11 DCs dc NNS cord-266018-8bhnlsgy 192 12 were be VBD cord-266018-8bhnlsgy 192 13 primarily primarily RB cord-266018-8bhnlsgy 192 14 responsible responsible JJ cord-266018-8bhnlsgy 192 15 for for IN cord-266018-8bhnlsgy 192 16 production production NN cord-266018-8bhnlsgy 192 17 of of IN cord-266018-8bhnlsgy 192 18 IL-12 IL-12 NNP cord-266018-8bhnlsgy 192 19 and and CC cord-266018-8bhnlsgy 192 20 contributing contribute VBG cord-266018-8bhnlsgy 192 21 to to IN cord-266018-8bhnlsgy 192 22 a a DT cord-266018-8bhnlsgy 192 23 Th1 th1 CD cord-266018-8bhnlsgy 192 24 response response NN cord-266018-8bhnlsgy 192 25 . . . cord-266018-8bhnlsgy 193 1 However however RB cord-266018-8bhnlsgy 193 2 , , , cord-266018-8bhnlsgy 193 3 recent recent JJ cord-266018-8bhnlsgy 193 4 studies study NNS cord-266018-8bhnlsgy 193 5 have have VBP cord-266018-8bhnlsgy 193 6 indicated indicate VBN cord-266018-8bhnlsgy 193 7 that that IN cord-266018-8bhnlsgy 193 8 CD8a CD8a NNP cord-266018-8bhnlsgy 193 9 À À HYPH cord-266018-8bhnlsgy 193 10 DCs dc NNS cord-266018-8bhnlsgy 193 11 also also RB cord-266018-8bhnlsgy 193 12 have have VBP cord-266018-8bhnlsgy 193 13 the the DT cord-266018-8bhnlsgy 193 14 potential potential NN cord-266018-8bhnlsgy 193 15 for for IN cord-266018-8bhnlsgy 193 16 secreting secrete VBG cord-266018-8bhnlsgy 193 17 IL-12 IL-12 NNP cord-266018-8bhnlsgy 193 18 and and CC cord-266018-8bhnlsgy 193 19 influencing influence VBG cord-266018-8bhnlsgy 193 20 the the DT cord-266018-8bhnlsgy 193 21 T T NNP cord-266018-8bhnlsgy 193 22 cell cell NN cord-266018-8bhnlsgy 193 23 response response NN cord-266018-8bhnlsgy 193 24 ( ( -LRB- cord-266018-8bhnlsgy 193 25 Doxsee Doxsee NNP cord-266018-8bhnlsgy 193 26 et et FW cord-266018-8bhnlsgy 193 27 al al NNP cord-266018-8bhnlsgy 193 28 . . NNP cord-266018-8bhnlsgy 193 29 , , , cord-266018-8bhnlsgy 193 30 2003 2003 CD cord-266018-8bhnlsgy 193 31 ) ) -RRB- cord-266018-8bhnlsgy 193 32 . . . cord-266018-8bhnlsgy 194 1 Our -PRON- PRP$ cord-266018-8bhnlsgy 194 2 studies study NNS cord-266018-8bhnlsgy 194 3 clearly clearly RB cord-266018-8bhnlsgy 194 4 indicate indicate VBP cord-266018-8bhnlsgy 194 5 that that IN cord-266018-8bhnlsgy 194 6 CD8a CD8a NNP cord-266018-8bhnlsgy 194 7 À À HYPH cord-266018-8bhnlsgy 194 8 DCs dc NNS cord-266018-8bhnlsgy 194 9 isolated isolate VBN cord-266018-8bhnlsgy 194 10 from from IN cord-266018-8bhnlsgy 194 11 the the DT cord-266018-8bhnlsgy 194 12 draining drain VBG cord-266018-8bhnlsgy 194 13 CLN cln NN cord-266018-8bhnlsgy 194 14 of of IN cord-266018-8bhnlsgy 194 15 MHV MHV NNP cord-266018-8bhnlsgy 194 16 - - HYPH cord-266018-8bhnlsgy 194 17 infected infect VBN cord-266018-8bhnlsgy 194 18 CCL3 CCL3 NNP cord-266018-8bhnlsgy 194 19 + + CC cord-266018-8bhnlsgy 194 20 /+ /+ . cord-266018-8bhnlsgy 195 1 mice mouse NNS cord-266018-8bhnlsgy 195 2 secrete secrete JJ cord-266018-8bhnlsgy 195 3 IL-12 IL-12 NNP cord-266018-8bhnlsgy 195 4 suggesting suggest VBG cord-266018-8bhnlsgy 195 5 that that IN cord-266018-8bhnlsgy 195 6 these these DT cord-266018-8bhnlsgy 195 7 cells cell NNS cord-266018-8bhnlsgy 195 8 help help VBP cord-266018-8bhnlsgy 195 9 influence influence VB cord-266018-8bhnlsgy 195 10 a a DT cord-266018-8bhnlsgy 195 11 protective protective JJ cord-266018-8bhnlsgy 195 12 Th1-mediated Th1-mediated NNP cord-266018-8bhnlsgy 195 13 immune immune JJ cord-266018-8bhnlsgy 195 14 response response NN cord-266018-8bhnlsgy 195 15 characterized characterize VBN cord-266018-8bhnlsgy 195 16 by by IN cord-266018-8bhnlsgy 195 17 the the DT cord-266018-8bhnlsgy 195 18 majority majority NN cord-266018-8bhnlsgy 195 19 of of IN cord-266018-8bhnlsgy 195 20 antigen antigen NN cord-266018-8bhnlsgy 195 21 - - HYPH cord-266018-8bhnlsgy 195 22 specific specific JJ cord-266018-8bhnlsgy 195 23 T t NN cord-266018-8bhnlsgy 195 24 cells cell NNS cord-266018-8bhnlsgy 195 25 expressing express VBG cord-266018-8bhnlsgy 195 26 IFN IFN NNP cord-266018-8bhnlsgy 195 27 - - HYPH cord-266018-8bhnlsgy 195 28 g g NNP cord-266018-8bhnlsgy 195 29 rather rather RB cord-266018-8bhnlsgy 195 30 than than IN cord-266018-8bhnlsgy 195 31 IL-10 IL-10 NNP cord-266018-8bhnlsgy 195 32 ( ( -LRB- cord-266018-8bhnlsgy 195 33 Table Table NNP cord-266018-8bhnlsgy 195 34 1 1 CD cord-266018-8bhnlsgy 195 35 ) ) -RRB- cord-266018-8bhnlsgy 195 36 . . . cord-266018-8bhnlsgy 196 1 In in IN cord-266018-8bhnlsgy 196 2 stark stark JJ cord-266018-8bhnlsgy 196 3 contrast contrast NN cord-266018-8bhnlsgy 196 4 is be VBZ cord-266018-8bhnlsgy 196 5 the the DT cord-266018-8bhnlsgy 196 6 data datum NNS cord-266018-8bhnlsgy 196 7 indicating indicate VBG cord-266018-8bhnlsgy 196 8 that that IN cord-266018-8bhnlsgy 196 9 CD8a CD8a NNP cord-266018-8bhnlsgy 196 10 À À HYPH cord-266018-8bhnlsgy 196 11 DCs dc NNS cord-266018-8bhnlsgy 196 12 present present NN cord-266018-8bhnlsgy 196 13 in in IN cord-266018-8bhnlsgy 196 14 the the DT cord-266018-8bhnlsgy 196 15 CLN cln NN cord-266018-8bhnlsgy 196 16 of of IN cord-266018-8bhnlsgy 196 17 infected infect VBN cord-266018-8bhnlsgy 196 18 CCL3 CCL3 NNP cord-266018-8bhnlsgy 196 19 À à NN cord-266018-8bhnlsgy 196 20 / / SYM cord-266018-8bhnlsgy 196 21 À à NN cord-266018-8bhnlsgy 196 22 mice mouse NNS cord-266018-8bhnlsgy 196 23 predominantly predominantly RB cord-266018-8bhnlsgy 196 24 secrete secrete VBP cord-266018-8bhnlsgy 196 25 IL-10 IL-10 NNP cord-266018-8bhnlsgy 196 26 and and CC cord-266018-8bhnlsgy 196 27 this this DT cord-266018-8bhnlsgy 196 28 correlates correlate VBZ cord-266018-8bhnlsgy 196 29 with with IN cord-266018-8bhnlsgy 196 30 limited limited JJ cord-266018-8bhnlsgy 196 31 IFN IFN NNP cord-266018-8bhnlsgy 196 32 - - HYPH cord-266018-8bhnlsgy 196 33 g g NN cord-266018-8bhnlsgy 196 34 expression expression NN cord-266018-8bhnlsgy 196 35 and and CC cord-266018-8bhnlsgy 196 36 enhanced enhanced JJ cord-266018-8bhnlsgy 196 37 expression expression NN cord-266018-8bhnlsgy 196 38 of of IN cord-266018-8bhnlsgy 196 39 the the DT cord-266018-8bhnlsgy 196 40 Th2-associated th2-associated JJ cord-266018-8bhnlsgy 196 41 cytokine cytokine NN cord-266018-8bhnlsgy 196 42 IL-10 IL-10 NNP cord-266018-8bhnlsgy 196 43 ( ( -LRB- cord-266018-8bhnlsgy 196 44 Table table NN cord-266018-8bhnlsgy 196 45 1 1 CD cord-266018-8bhnlsgy 196 46 ) ) -RRB- cord-266018-8bhnlsgy 196 47 . . . cord-266018-8bhnlsgy 197 1 Therefore therefore RB cord-266018-8bhnlsgy 197 2 , , , cord-266018-8bhnlsgy 197 3 the the DT cord-266018-8bhnlsgy 197 4 data datum NNS cord-266018-8bhnlsgy 197 5 indicate indicate VBP cord-266018-8bhnlsgy 197 6 that that IN cord-266018-8bhnlsgy 197 7 cytokine cytokine NN cord-266018-8bhnlsgy 197 8 production production NN cord-266018-8bhnlsgy 197 9 , , , cord-266018-8bhnlsgy 197 10 rather rather RB cord-266018-8bhnlsgy 197 11 than than IN cord-266018-8bhnlsgy 197 12 the the DT cord-266018-8bhnlsgy 197 13 type type NN cord-266018-8bhnlsgy 197 14 of of IN cord-266018-8bhnlsgy 197 15 CD11c CD11c NNP cord-266018-8bhnlsgy 197 16 + + CC cord-266018-8bhnlsgy 197 17 cell cell NN cord-266018-8bhnlsgy 197 18 , , , cord-266018-8bhnlsgy 197 19 may may MD cord-266018-8bhnlsgy 197 20 control control VB cord-266018-8bhnlsgy 197 21 the the DT cord-266018-8bhnlsgy 197 22 predominant predominant JJ cord-266018-8bhnlsgy 197 23 T T NNP cord-266018-8bhnlsgy 197 24 cell cell NN cord-266018-8bhnlsgy 197 25 immune immune JJ cord-266018-8bhnlsgy 197 26 response response NN cord-266018-8bhnlsgy 197 27 within within IN cord-266018-8bhnlsgy 197 28 the the DT cord-266018-8bhnlsgy 197 29 CLN cln NN cord-266018-8bhnlsgy 197 30 . . . cord-266018-8bhnlsgy 198 1 Taken take VBN cord-266018-8bhnlsgy 198 2 together together RB cord-266018-8bhnlsgy 198 3 , , , cord-266018-8bhnlsgy 198 4 these these DT cord-266018-8bhnlsgy 198 5 data datum NNS cord-266018-8bhnlsgy 198 6 point point NN cord-266018-8bhnlsgy 198 7 to to IN cord-266018-8bhnlsgy 198 8 an an DT cord-266018-8bhnlsgy 198 9 important important JJ cord-266018-8bhnlsgy 198 10 role role NN cord-266018-8bhnlsgy 198 11 in in IN cord-266018-8bhnlsgy 198 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 198 13 expression expression NN cord-266018-8bhnlsgy 198 14 in in IN cord-266018-8bhnlsgy 198 15 linking link VBG cord-266018-8bhnlsgy 198 16 innate innate JJ cord-266018-8bhnlsgy 198 17 and and CC cord-266018-8bhnlsgy 198 18 adaptive adaptive JJ cord-266018-8bhnlsgy 198 19 immune immune JJ cord-266018-8bhnlsgy 198 20 responses response NNS cord-266018-8bhnlsgy 198 21 following follow VBG cord-266018-8bhnlsgy 198 22 viral viral JJ cord-266018-8bhnlsgy 198 23 infection infection NN cord-266018-8bhnlsgy 198 24 of of IN cord-266018-8bhnlsgy 198 25 the the DT cord-266018-8bhnlsgy 198 26 CNS CNS NNP cord-266018-8bhnlsgy 198 27 by by IN cord-266018-8bhnlsgy 198 28 contributing contribute VBG cord-266018-8bhnlsgy 198 29 to to IN cord-266018-8bhnlsgy 198 30 the the DT cord-266018-8bhnlsgy 198 31 activation activation NN cord-266018-8bhnlsgy 198 32 Fig Fig NNP cord-266018-8bhnlsgy 198 33 . . . cord-266018-8bhnlsgy 199 1 3 3 LS cord-266018-8bhnlsgy 199 2 . . . cord-266018-8bhnlsgy 200 1 Cytokine cytokine NN cord-266018-8bhnlsgy 200 2 and and CC cord-266018-8bhnlsgy 200 3 chemokine chemokine JJ cord-266018-8bhnlsgy 200 4 secretion secretion NN cord-266018-8bhnlsgy 200 5 by by IN cord-266018-8bhnlsgy 200 6 CD11c CD11c NNP cord-266018-8bhnlsgy 201 1 + + CC cord-266018-8bhnlsgy 202 1 CD11b CD11b NNS cord-266018-8bhnlsgy 202 2 + + CC cord-266018-8bhnlsgy 203 1 CD8a CD8a NNP cord-266018-8bhnlsgy 203 2 À à NN cord-266018-8bhnlsgy 203 3 cells cell NNS cord-266018-8bhnlsgy 203 4 isolated isolate VBN cord-266018-8bhnlsgy 203 5 from from IN cord-266018-8bhnlsgy 203 6 mononuclear mononuclear JJ cord-266018-8bhnlsgy 203 7 cells cell NNS cord-266018-8bhnlsgy 203 8 pooled pool VBN cord-266018-8bhnlsgy 203 9 from from IN cord-266018-8bhnlsgy 203 10 the the DT cord-266018-8bhnlsgy 203 11 CLN cln NN cord-266018-8bhnlsgy 203 12 of of IN cord-266018-8bhnlsgy 203 13 MHV MHV NNP cord-266018-8bhnlsgy 203 14 - - HYPH cord-266018-8bhnlsgy 203 15 infected infect VBN cord-266018-8bhnlsgy 203 16 CCL3 CCL3 NNP cord-266018-8bhnlsgy 203 17 + + CC cord-266018-8bhnlsgy 203 18 /+ /+ . cord-266018-8bhnlsgy 204 1 and and CC cord-266018-8bhnlsgy 204 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 204 3 À À NNP cord-266018-8bhnlsgy 204 4 / / SYM cord-266018-8bhnlsgy 204 5 À à NN cord-266018-8bhnlsgy 204 6 mice mouse NNS cord-266018-8bhnlsgy 204 7 at at IN cord-266018-8bhnlsgy 204 8 day day NN cord-266018-8bhnlsgy 205 1 7 7 CD cord-266018-8bhnlsgy 205 2 p.i p.i NNP cord-266018-8bhnlsgy 205 3 . . . cord-266018-8bhnlsgy 205 4 Supernatants supernatant NNS cord-266018-8bhnlsgy 205 5 were be VBD cord-266018-8bhnlsgy 205 6 analyzed analyze VBN cord-266018-8bhnlsgy 205 7 for for IN cord-266018-8bhnlsgy 205 8 the the DT cord-266018-8bhnlsgy 205 9 production production NN cord-266018-8bhnlsgy 205 10 of of IN cord-266018-8bhnlsgy 205 11 IL-12p70 IL-12p70 NNP cord-266018-8bhnlsgy 205 12 , , , cord-266018-8bhnlsgy 205 13 IL-10 IL-10 NNP cord-266018-8bhnlsgy 205 14 , , , cord-266018-8bhnlsgy 205 15 and and CC cord-266018-8bhnlsgy 205 16 CCL3 CCL3 NNP cord-266018-8bhnlsgy 205 17 by by IN cord-266018-8bhnlsgy 205 18 ELISA ELISA NNP cord-266018-8bhnlsgy 205 19 24 24 CD cord-266018-8bhnlsgy 205 20 h h NN cord-266018-8bhnlsgy 205 21 following follow VBG cord-266018-8bhnlsgy 205 22 culture culture NN cord-266018-8bhnlsgy 205 23 . . . cord-266018-8bhnlsgy 206 1 A a DT cord-266018-8bhnlsgy 206 2 minimum minimum NN cord-266018-8bhnlsgy 206 3 of of IN cord-266018-8bhnlsgy 206 4 three three CD cord-266018-8bhnlsgy 206 5 to to TO cord-266018-8bhnlsgy 206 6 six six CD cord-266018-8bhnlsgy 206 7 mice mouse NNS cord-266018-8bhnlsgy 206 8 per per IN cord-266018-8bhnlsgy 206 9 group group NN cord-266018-8bhnlsgy 206 10 were be VBD cord-266018-8bhnlsgy 206 11 used use VBN cord-266018-8bhnlsgy 206 12 for for IN cord-266018-8bhnlsgy 206 13 isolation isolation NN cord-266018-8bhnlsgy 206 14 of of IN cord-266018-8bhnlsgy 206 15 cells cell NNS cord-266018-8bhnlsgy 206 16 and and CC cord-266018-8bhnlsgy 206 17 data datum NNS cord-266018-8bhnlsgy 206 18 presented present VBN cord-266018-8bhnlsgy 206 19 indicate indicate VBP cord-266018-8bhnlsgy 206 20 the the DT cord-266018-8bhnlsgy 206 21 average average JJ cord-266018-8bhnlsgy 206 22 F F NNP cord-266018-8bhnlsgy 206 23 SD SD NNP cord-266018-8bhnlsgy 206 24 . . . cord-266018-8bhnlsgy 207 1 * * NFP cord-266018-8bhnlsgy 207 2 P p NN cord-266018-8bhnlsgy 207 3 V v NN cord-266018-8bhnlsgy 207 4 0.001 0.001 CD cord-266018-8bhnlsgy 207 5 . . . cord-266018-8bhnlsgy 208 1 Table table NN cord-266018-8bhnlsgy 208 2 1 1 CD cord-266018-8bhnlsgy 208 3 Frequency frequency NN cord-266018-8bhnlsgy 208 4 of of IN cord-266018-8bhnlsgy 208 5 cytokine cytokine NN cord-266018-8bhnlsgy 208 6 - - HYPH cord-266018-8bhnlsgy 208 7 producing produce VBG cord-266018-8bhnlsgy 208 8 T t NN cord-266018-8bhnlsgy 208 9 cells cell NNS cord-266018-8bhnlsgy 208 10 present present JJ cord-266018-8bhnlsgy 208 11 within within IN cord-266018-8bhnlsgy 208 12 draining drain VBG cord-266018-8bhnlsgy 208 13 cervical cervical JJ cord-266018-8bhnlsgy 208 14 lymph lymph NN cord-266018-8bhnlsgy 208 15 nodes node NNS cord-266018-8bhnlsgy 208 16 following follow VBG cord-266018-8bhnlsgy 208 17 MHV MHV NNP cord-266018-8bhnlsgy 208 18 infection infection NN cord-266018-8bhnlsgy 208 19 a a DT cord-266018-8bhnlsgy 208 20 Day day NN cord-266018-8bhnlsgy 209 1 p.i p.i NNP cord-266018-8bhnlsgy 209 2 . . . cord-266018-8bhnlsgy 209 3 IFN IFN NNP cord-266018-8bhnlsgy 209 4 - - HYPH cord-266018-8bhnlsgy 209 5 g g NNP cord-266018-8bhnlsgy 209 6 IL-2 il-2 NN cord-266018-8bhnlsgy 209 7 IL-10 IL-10 NNP cord-266018-8bhnlsgy 209 8 CD4 CD4 NNP cord-266018-8bhnlsgy 209 9 + + CC cord-266018-8bhnlsgy 209 10 T t NN cord-266018-8bhnlsgy 209 11 cells cell NNS cord-266018-8bhnlsgy 209 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 209 13 + + CC cord-266018-8bhnlsgy 210 1 /+ /+ . cord-266018-8bhnlsgy 211 1 7 7 CD cord-266018-8bhnlsgy 211 2 2 2 CD cord-266018-8bhnlsgy 211 3 0F 0f CD cord-266018-8bhnlsgy 211 4 4 4 CD cord-266018-8bhnlsgy 211 5 35F 35f CD cord-266018-8bhnlsgy 211 6 5 5 CD cord-266018-8bhnlsgy 211 7 5F 5f CD cord-266018-8bhnlsgy 212 1 2 2 CD cord-266018-8bhnlsgy 212 2 12 12 CD cord-266018-8bhnlsgy 212 3 Data datum NNS cord-266018-8bhnlsgy 212 4 represent represent VBP cord-266018-8bhnlsgy 212 5 two two CD cord-266018-8bhnlsgy 212 6 separate separate JJ cord-266018-8bhnlsgy 212 7 experiments experiment NNS cord-266018-8bhnlsgy 212 8 with with IN cord-266018-8bhnlsgy 212 9 at at RB cord-266018-8bhnlsgy 212 10 least least RBS cord-266018-8bhnlsgy 212 11 three three CD cord-266018-8bhnlsgy 212 12 mice mouse NNS cord-266018-8bhnlsgy 212 13 per per IN cord-266018-8bhnlsgy 212 14 group group NN cord-266018-8bhnlsgy 212 15 , , , cord-266018-8bhnlsgy 212 16 n n NN cord-266018-8bhnlsgy 212 17 = = SYM cord-266018-8bhnlsgy 212 18 6 6 CD cord-266018-8bhnlsgy 212 19 . . . cord-266018-8bhnlsgy 213 1 Data datum NNS cord-266018-8bhnlsgy 213 2 are be VBP cord-266018-8bhnlsgy 213 3 presented present VBN cord-266018-8bhnlsgy 213 4 as as IN cord-266018-8bhnlsgy 213 5 average average JJ cord-266018-8bhnlsgy 213 6 F F NNP cord-266018-8bhnlsgy 213 7 SD sd NN cord-266018-8bhnlsgy 213 8 . . . cord-266018-8bhnlsgy 214 1 a a DT cord-266018-8bhnlsgy 214 2 Cytokine cytokine NN cord-266018-8bhnlsgy 214 3 expression expression NN cord-266018-8bhnlsgy 214 4 determined determine VBN cord-266018-8bhnlsgy 214 5 by by IN cord-266018-8bhnlsgy 214 6 pooling pool VBG cord-266018-8bhnlsgy 214 7 cells cell NNS cord-266018-8bhnlsgy 214 8 from from IN cord-266018-8bhnlsgy 214 9 draining drain VBG cord-266018-8bhnlsgy 214 10 CLN cln NN cord-266018-8bhnlsgy 214 11 of of IN cord-266018-8bhnlsgy 214 12 MHV MHV NNP cord-266018-8bhnlsgy 214 13 - - HYPH cord-266018-8bhnlsgy 214 14 infected infect VBN cord-266018-8bhnlsgy 214 15 mice mouse NNS cord-266018-8bhnlsgy 214 16 at at IN cord-266018-8bhnlsgy 214 17 defined define VBN cord-266018-8bhnlsgy 214 18 times times NNP cord-266018-8bhnlsgy 214 19 p.i p.i NNP cord-266018-8bhnlsgy 214 20 . . NNP cord-266018-8bhnlsgy 214 21 and and CC cord-266018-8bhnlsgy 214 22 pulsing pulse VBG cord-266018-8bhnlsgy 214 23 with with IN cord-266018-8bhnlsgy 214 24 defined define VBN cord-266018-8bhnlsgy 214 25 CD4 CD4 NNP cord-266018-8bhnlsgy 214 26 + + CC cord-266018-8bhnlsgy 214 27 epitope epitope NN cord-266018-8bhnlsgy 214 28 ( ( -LRB- cord-266018-8bhnlsgy 214 29 M133 M133 NNP cord-266018-8bhnlsgy 214 30 - - HYPH cord-266018-8bhnlsgy 214 31 147 147 CD cord-266018-8bhnlsgy 214 32 ) ) -RRB- cord-266018-8bhnlsgy 214 33 and and CC cord-266018-8bhnlsgy 214 34 CD8 CD8 NNP cord-266018-8bhnlsgy 214 35 + + CC cord-266018-8bhnlsgy 214 36 epitope epitope NNP cord-266018-8bhnlsgy 214 37 ( ( -LRB- cord-266018-8bhnlsgy 214 38 S510 S510 NNP cord-266018-8bhnlsgy 214 39 - - HYPH cord-266018-8bhnlsgy 214 40 518 518 CD cord-266018-8bhnlsgy 214 41 ) ) -RRB- cord-266018-8bhnlsgy 214 42 . . . cord-266018-8bhnlsgy 215 1 b b LS cord-266018-8bhnlsgy 215 2 P p NN cord-266018-8bhnlsgy 215 3 V v NN cord-266018-8bhnlsgy 215 4 0.01 0.01 CD cord-266018-8bhnlsgy 215 5 . . . cord-266018-8bhnlsgy 216 1 Decreased decrease VBN cord-266018-8bhnlsgy 216 2 frequency frequency NN cord-266018-8bhnlsgy 216 3 of of IN cord-266018-8bhnlsgy 216 4 IFN IFN NNP cord-266018-8bhnlsgy 216 5 - - HYPH cord-266018-8bhnlsgy 216 6 g g NNP cord-266018-8bhnlsgy 216 7 secreting secrete VBG cord-266018-8bhnlsgy 216 8 T t NN cord-266018-8bhnlsgy 216 9 cells cell NNS cord-266018-8bhnlsgy 216 10 and and CC cord-266018-8bhnlsgy 216 11 increased increase VBN cord-266018-8bhnlsgy 216 12 frequency frequency NN cord-266018-8bhnlsgy 216 13 of of IN cord-266018-8bhnlsgy 216 14 IL-2 il-2 NN cord-266018-8bhnlsgy 216 15 producing produce VBG cord-266018-8bhnlsgy 216 16 T t NN cord-266018-8bhnlsgy 216 17 cells cell NNS cord-266018-8bhnlsgy 216 18 following follow VBG cord-266018-8bhnlsgy 216 19 MHV MHV NNP cord-266018-8bhnlsgy 216 20 infection infection NN cord-266018-8bhnlsgy 216 21 of of IN cord-266018-8bhnlsgy 216 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 216 23 À à NN cord-266018-8bhnlsgy 216 24 / / SYM cord-266018-8bhnlsgy 216 25 À à NN cord-266018-8bhnlsgy 216 26 mice mouse NNS cord-266018-8bhnlsgy 216 27 as as IN cord-266018-8bhnlsgy 216 28 compared compare VBN cord-266018-8bhnlsgy 216 29 to to IN cord-266018-8bhnlsgy 216 30 CCL3 CCL3 NNP cord-266018-8bhnlsgy 216 31 + + CC cord-266018-8bhnlsgy 216 32 /+ /+ . cord-266018-8bhnlsgy 216 33 mice mouse NNS cord-266018-8bhnlsgy 216 34 . . . cord-266018-8bhnlsgy 217 1 c c NN cord-266018-8bhnlsgy 217 2 P p NN cord-266018-8bhnlsgy 217 3 V v NN cord-266018-8bhnlsgy 217 4 0.002 0.002 CD cord-266018-8bhnlsgy 217 5 . . . cord-266018-8bhnlsgy 218 1 Increased increase VBN cord-266018-8bhnlsgy 218 2 frequency frequency NN cord-266018-8bhnlsgy 218 3 of of IN cord-266018-8bhnlsgy 218 4 IL-10 IL-10 NNP cord-266018-8bhnlsgy 218 5 producing produce VBG cord-266018-8bhnlsgy 218 6 T t NN cord-266018-8bhnlsgy 218 7 cells cell NNS cord-266018-8bhnlsgy 218 8 in in IN cord-266018-8bhnlsgy 218 9 CCL3 CCL3 NNP cord-266018-8bhnlsgy 218 10 À à NN cord-266018-8bhnlsgy 218 11 / / SYM cord-266018-8bhnlsgy 218 12 À à NN cord-266018-8bhnlsgy 218 13 mice mouse NNS cord-266018-8bhnlsgy 218 14 as as IN cord-266018-8bhnlsgy 218 15 compared compare VBN cord-266018-8bhnlsgy 218 16 to to IN cord-266018-8bhnlsgy 218 17 CCL3 CCL3 NNP cord-266018-8bhnlsgy 218 18 + + CC cord-266018-8bhnlsgy 218 19 /+ /+ . cord-266018-8bhnlsgy 219 1 mice mouse NNS cord-266018-8bhnlsgy 219 2 following follow VBG cord-266018-8bhnlsgy 219 3 MHV MHV NNP cord-266018-8bhnlsgy 219 4 infection infection NN cord-266018-8bhnlsgy 219 5 . . . cord-266018-8bhnlsgy 220 1 of of IN cord-266018-8bhnlsgy 220 2 DCs DCs NNP cord-266018-8bhnlsgy 220 3 through through IN cord-266018-8bhnlsgy 220 4 regulating regulate VBG cord-266018-8bhnlsgy 220 5 the the DT cord-266018-8bhnlsgy 220 6 migration migration NN cord-266018-8bhnlsgy 220 7 of of IN cord-266018-8bhnlsgy 220 8 cells cell NNS cord-266018-8bhnlsgy 220 9 from from IN cord-266018-8bhnlsgy 220 10 the the DT cord-266018-8bhnlsgy 220 11 CNS CNS NNP cord-266018-8bhnlsgy 220 12 to to TO cord-266018-8bhnlsgy 220 13 lymphoid lymphoid JJ cord-266018-8bhnlsgy 220 14 tissues tissue NNS cord-266018-8bhnlsgy 220 15 as as RB cord-266018-8bhnlsgy 220 16 well well RB cord-266018-8bhnlsgy 220 17 as as IN cord-266018-8bhnlsgy 220 18 the the DT cord-266018-8bhnlsgy 220 19 expression expression NN cord-266018-8bhnlsgy 220 20 of of IN cord-266018-8bhnlsgy 220 21 both both DT cord-266018-8bhnlsgy 220 22 co co JJ cord-266018-8bhnlsgy 220 23 - - JJ cord-266018-8bhnlsgy 220 24 stimulatory stimulatory JJ cord-266018-8bhnlsgy 220 25 molecules molecule NNS cord-266018-8bhnlsgy 220 26 and and CC cord-266018-8bhnlsgy 220 27 cytokine cytokine NN cord-266018-8bhnlsgy 220 28 production production NN cord-266018-8bhnlsgy 220 29 . . . cord-266018-8bhnlsgy 221 1 However however RB cord-266018-8bhnlsgy 221 2 , , , cord-266018-8bhnlsgy 221 3 it -PRON- PRP cord-266018-8bhnlsgy 221 4 is be VBZ cord-266018-8bhnlsgy 221 5 important important JJ cord-266018-8bhnlsgy 221 6 to to TO cord-266018-8bhnlsgy 221 7 note note VB cord-266018-8bhnlsgy 221 8 that that IN cord-266018-8bhnlsgy 221 9 there there EX cord-266018-8bhnlsgy 221 10 is be VBZ cord-266018-8bhnlsgy 221 11 the the DT cord-266018-8bhnlsgy 221 12 possibility possibility NN cord-266018-8bhnlsgy 221 13 of of IN cord-266018-8bhnlsgy 221 14 other other JJ cord-266018-8bhnlsgy 221 15 chemokines chemokine NNS cord-266018-8bhnlsgy 221 16 and and CC cord-266018-8bhnlsgy 221 17 chemokine chemokine NN cord-266018-8bhnlsgy 221 18 receptors receptor NNS cord-266018-8bhnlsgy 221 19 participating participate VBG cord-266018-8bhnlsgy 221 20 in in IN cord-266018-8bhnlsgy 221 21 DC DC NNP cord-266018-8bhnlsgy 221 22 responses response NNS cord-266018-8bhnlsgy 221 23 following follow VBG cord-266018-8bhnlsgy 221 24 viral viral JJ cord-266018-8bhnlsgy 221 25 infection infection NN cord-266018-8bhnlsgy 221 26 . . . cord-266018-8bhnlsgy 222 1 Indeed indeed RB cord-266018-8bhnlsgy 222 2 , , , cord-266018-8bhnlsgy 222 3 the the DT cord-266018-8bhnlsgy 222 4 CC cc NN cord-266018-8bhnlsgy 222 5 chemokine chemokine NN cord-266018-8bhnlsgy 222 6 receptor receptor NN cord-266018-8bhnlsgy 222 7 2 2 CD cord-266018-8bhnlsgy 222 8 ( ( -LRB- cord-266018-8bhnlsgy 222 9 CCR2 CCR2 NNP cord-266018-8bhnlsgy 222 10 ) ) -RRB- cord-266018-8bhnlsgy 222 11 is be VBZ cord-266018-8bhnlsgy 222 12 expressed express VBN cord-266018-8bhnlsgy 222 13 on on IN cord-266018-8bhnlsgy 222 14 professional professional JJ cord-266018-8bhnlsgy 222 15 APC APC NNP cord-266018-8bhnlsgy 222 16 including include VBG cord-266018-8bhnlsgy 222 17 macrophages macrophage NNS cord-266018-8bhnlsgy 222 18 and and CC cord-266018-8bhnlsgy 222 19 DCs dc NNS cord-266018-8bhnlsgy 222 20 and and CC cord-266018-8bhnlsgy 222 21 is be VBZ cord-266018-8bhnlsgy 222 22 thought think VBN cord-266018-8bhnlsgy 222 23 to to TO cord-266018-8bhnlsgy 222 24 contribute contribute VB cord-266018-8bhnlsgy 222 25 to to IN cord-266018-8bhnlsgy 222 26 defense defense NN cord-266018-8bhnlsgy 222 27 following follow VBG cord-266018-8bhnlsgy 222 28 microbial microbial JJ cord-266018-8bhnlsgy 222 29 challenge challenge NN cord-266018-8bhnlsgy 222 30 by by IN cord-266018-8bhnlsgy 222 31 enhancing enhance VBG cord-266018-8bhnlsgy 222 32 recruitment recruitment NN cord-266018-8bhnlsgy 222 33 as as RB cord-266018-8bhnlsgy 222 34 well well RB cord-266018-8bhnlsgy 222 35 as as IN cord-266018-8bhnlsgy 222 36 production production NN cord-266018-8bhnlsgy 222 37 of of IN cord-266018-8bhnlsgy 222 38 antimicrobial antimicrobial JJ cord-266018-8bhnlsgy 222 39 products product NNS cord-266018-8bhnlsgy 222 40 such such JJ cord-266018-8bhnlsgy 222 41 as as IN cord-266018-8bhnlsgy 222 42 TNF TNF NNP cord-266018-8bhnlsgy 222 43 - - : cord-266018-8bhnlsgy 222 44 a a DT cord-266018-8bhnlsgy 223 1 and and CC cord-266018-8bhnlsgy 223 2 NO no RB cord-266018-8bhnlsgy 223 3 by by IN cord-266018-8bhnlsgy 223 4 these these DT cord-266018-8bhnlsgy 223 5 cells cell NNS cord-266018-8bhnlsgy 223 6 ( ( -LRB- cord-266018-8bhnlsgy 223 7 Luster Luster NNP cord-266018-8bhnlsgy 223 8 , , , cord-266018-8bhnlsgy 223 9 2002 2002 CD cord-266018-8bhnlsgy 223 10 ; ; : cord-266018-8bhnlsgy 224 1 McColl McColl NNP cord-266018-8bhnlsgy 224 2 , , , cord-266018-8bhnlsgy 224 3 2002 2002 CD cord-266018-8bhnlsgy 224 4 ; ; : cord-266018-8bhnlsgy 224 5 Serbina Serbina NNP cord-266018-8bhnlsgy 224 6 et et NNP cord-266018-8bhnlsgy 224 7 al al NNP cord-266018-8bhnlsgy 224 8 . . NNP cord-266018-8bhnlsgy 224 9 , , , cord-266018-8bhnlsgy 224 10 2003 2003 CD cord-266018-8bhnlsgy 224 11 ) ) -RRB- cord-266018-8bhnlsgy 224 12 . . . cord-266018-8bhnlsgy 225 1 In in IN cord-266018-8bhnlsgy 225 2 addition addition NN cord-266018-8bhnlsgy 225 3 , , , cord-266018-8bhnlsgy 225 4 the the DT cord-266018-8bhnlsgy 225 5 absence absence NN cord-266018-8bhnlsgy 225 6 of of IN cord-266018-8bhnlsgy 225 7 CCR2 CCR2 NNP cord-266018-8bhnlsgy 225 8 signaling signal VBG cord-266018-8bhnlsgy 225 9 results result NNS cord-266018-8bhnlsgy 225 10 in in IN cord-266018-8bhnlsgy 225 11 diminished diminished JJ cord-266018-8bhnlsgy 225 12 trafficking trafficking NN cord-266018-8bhnlsgy 225 13 and and CC cord-266018-8bhnlsgy 225 14 accumulation accumulation NN cord-266018-8bhnlsgy 225 15 of of IN cord-266018-8bhnlsgy 225 16 dendritic dendritic JJ cord-266018-8bhnlsgy 225 17 cells cell NNS cord-266018-8bhnlsgy 225 18 within within IN cord-266018-8bhnlsgy 225 19 secondary secondary JJ cord-266018-8bhnlsgy 225 20 lymphoid lymphoid JJ cord-266018-8bhnlsgy 225 21 tissues tissue NNS cord-266018-8bhnlsgy 225 22 following follow VBG cord-266018-8bhnlsgy 225 23 antigenic antigenic JJ cord-266018-8bhnlsgy 225 24 challenge challenge NN cord-266018-8bhnlsgy 225 25 ( ( -LRB- cord-266018-8bhnlsgy 225 26 Peters Peters NNP cord-266018-8bhnlsgy 225 27 et et NNP cord-266018-8bhnlsgy 225 28 al al NNP cord-266018-8bhnlsgy 225 29 . . NNP cord-266018-8bhnlsgy 225 30 , , , cord-266018-8bhnlsgy 225 31 2000 2000 CD cord-266018-8bhnlsgy 226 1 ( ( -LRB- cord-266018-8bhnlsgy 226 2 Peters Peters NNP cord-266018-8bhnlsgy 226 3 et et NNP cord-266018-8bhnlsgy 226 4 al al NNP cord-266018-8bhnlsgy 226 5 . . . cord-266018-8bhnlsgy 227 1 , , , cord-266018-8bhnlsgy 227 2 , , , cord-266018-8bhnlsgy 227 3 2001 2001 CD cord-266018-8bhnlsgy 227 4 Sato Sato NNP cord-266018-8bhnlsgy 227 5 et et FW cord-266018-8bhnlsgy 227 6 al al NNP cord-266018-8bhnlsgy 227 7 . . NNP cord-266018-8bhnlsgy 227 8 , , , cord-266018-8bhnlsgy 227 9 2000 2000 CD cord-266018-8bhnlsgy 227 10 ) ) -RRB- cord-266018-8bhnlsgy 227 11 . . . cord-266018-8bhnlsgy 228 1 Studies study NNS cord-266018-8bhnlsgy 228 2 are be VBP cord-266018-8bhnlsgy 228 3 currently currently RB cord-266018-8bhnlsgy 228 4 in in IN cord-266018-8bhnlsgy 228 5 progress progress NN cord-266018-8bhnlsgy 228 6 to to TO cord-266018-8bhnlsgy 228 7 evaluate evaluate VB cord-266018-8bhnlsgy 228 8 the the DT cord-266018-8bhnlsgy 228 9 contributions contribution NNS cord-266018-8bhnlsgy 228 10 of of IN cord-266018-8bhnlsgy 228 11 additional additional JJ cord-266018-8bhnlsgy 228 12 chemokine chemokine NN cord-266018-8bhnlsgy 228 13 signaling signal VBG cord-266018-8bhnlsgy 228 14 pathways pathway NNS cord-266018-8bhnlsgy 228 15 that that WDT cord-266018-8bhnlsgy 228 16 may may MD cord-266018-8bhnlsgy 228 17 also also RB cord-266018-8bhnlsgy 228 18 participate participate VB cord-266018-8bhnlsgy 228 19 in in IN cord-266018-8bhnlsgy 228 20 DC DC NNP cord-266018-8bhnlsgy 228 21 activation activation NN cord-266018-8bhnlsgy 228 22 and and CC cord-266018-8bhnlsgy 228 23 migration migration NN cord-266018-8bhnlsgy 228 24 following follow VBG cord-266018-8bhnlsgy 228 25 coronavirus coronavirus NN cord-266018-8bhnlsgy 228 26 infection infection NN cord-266018-8bhnlsgy 228 27 . . . cord-266018-8bhnlsgy 229 1 In in IN cord-266018-8bhnlsgy 229 2 conclusion conclusion NN cord-266018-8bhnlsgy 229 3 , , , cord-266018-8bhnlsgy 229 4 the the DT cord-266018-8bhnlsgy 229 5 studies study NNS cord-266018-8bhnlsgy 229 6 presented present VBD cord-266018-8bhnlsgy 229 7 support support NN cord-266018-8bhnlsgy 229 8 and and CC cord-266018-8bhnlsgy 229 9 extend extend VB cord-266018-8bhnlsgy 229 10 previous previous JJ cord-266018-8bhnlsgy 229 11 work work NN cord-266018-8bhnlsgy 229 12 from from IN cord-266018-8bhnlsgy 229 13 our -PRON- PRP$ cord-266018-8bhnlsgy 229 14 laboratory laboratory NN cord-266018-8bhnlsgy 229 15 indicating indicate VBG cord-266018-8bhnlsgy 229 16 an an DT cord-266018-8bhnlsgy 229 17 important important JJ cord-266018-8bhnlsgy 229 18 role role NN cord-266018-8bhnlsgy 229 19 for for IN cord-266018-8bhnlsgy 229 20 chemokines chemokine NNS cord-266018-8bhnlsgy 229 21 in in IN cord-266018-8bhnlsgy 229 22 the the DT cord-266018-8bhnlsgy 229 23 migration migration NN cord-266018-8bhnlsgy 229 24 of of IN cord-266018-8bhnlsgy 229 25 T t NN cord-266018-8bhnlsgy 229 26 cells cell NNS cord-266018-8bhnlsgy 229 27 into into IN cord-266018-8bhnlsgy 229 28 the the DT cord-266018-8bhnlsgy 229 29 CNS CNS NNP cord-266018-8bhnlsgy 229 30 following follow VBG cord-266018-8bhnlsgy 229 31 MHV MHV NNP cord-266018-8bhnlsgy 229 32 infection infection NN cord-266018-8bhnlsgy 229 33 Glass Glass NNP cord-266018-8bhnlsgy 229 34 et et FW cord-266018-8bhnlsgy 229 35 al al NNP cord-266018-8bhnlsgy 229 36 . . NNP cord-266018-8bhnlsgy 229 37 , , , cord-266018-8bhnlsgy 229 38 2001 2001 CD cord-266018-8bhnlsgy 229 39 ; ; : cord-266018-8bhnlsgy 229 40 Liu Liu NNP cord-266018-8bhnlsgy 229 41 et et NNP cord-266018-8bhnlsgy 229 42 al al NNP cord-266018-8bhnlsgy 229 43 . . NNP cord-266018-8bhnlsgy 229 44 , , , cord-266018-8bhnlsgy 229 45 2001 2001 CD cord-266018-8bhnlsgy 229 46 ) ) -RRB- cord-266018-8bhnlsgy 229 47 . . . cord-266018-8bhnlsgy 230 1 Here here RB cord-266018-8bhnlsgy 230 2 , , , cord-266018-8bhnlsgy 230 3 we -PRON- PRP cord-266018-8bhnlsgy 230 4 have have VBP cord-266018-8bhnlsgy 230 5 demonstrated demonstrate VBN cord-266018-8bhnlsgy 230 6 a a DT cord-266018-8bhnlsgy 230 7 novel novel JJ cord-266018-8bhnlsgy 230 8 role role NN cord-266018-8bhnlsgy 230 9 for for IN cord-266018-8bhnlsgy 230 10 the the DT cord-266018-8bhnlsgy 230 11 chemokine chemokine NN cord-266018-8bhnlsgy 230 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 230 13 in in IN cord-266018-8bhnlsgy 230 14 enhancing enhance VBG cord-266018-8bhnlsgy 230 15 the the DT cord-266018-8bhnlsgy 230 16 accumulation accumulation NN cord-266018-8bhnlsgy 230 17 and and CC cord-266018-8bhnlsgy 230 18 activation activation NN cord-266018-8bhnlsgy 230 19 of of IN cord-266018-8bhnlsgy 230 20 CD8a CD8a NNP cord-266018-8bhnlsgy 230 21 À À HYPH cord-266018-8bhnlsgy 230 22 DCs dc NNS cord-266018-8bhnlsgy 230 23 within within IN cord-266018-8bhnlsgy 230 24 secondary secondary JJ cord-266018-8bhnlsgy 230 25 lymphoid lymphoid JJ cord-266018-8bhnlsgy 230 26 tissue tissue NN cord-266018-8bhnlsgy 230 27 and and CC cord-266018-8bhnlsgy 230 28 this this DT cord-266018-8bhnlsgy 230 29 correlates correlate VBZ cord-266018-8bhnlsgy 230 30 with with IN cord-266018-8bhnlsgy 230 31 altered alter VBN cord-266018-8bhnlsgy 230 32 T t NN cord-266018-8bhnlsgy 230 33 cell cell NN cord-266018-8bhnlsgy 230 34 activation activation NN cord-266018-8bhnlsgy 230 35 and and CC cord-266018-8bhnlsgy 230 36 differentiation differentiation NN cord-266018-8bhnlsgy 230 37 following follow VBG cord-266018-8bhnlsgy 230 38 viral viral JJ cord-266018-8bhnlsgy 230 39 infection infection NN cord-266018-8bhnlsgy 230 40 . . . cord-266018-8bhnlsgy 231 1 These these DT cord-266018-8bhnlsgy 231 2 results result NNS cord-266018-8bhnlsgy 231 3 indicate indicate VBP cord-266018-8bhnlsgy 231 4 that that IN cord-266018-8bhnlsgy 231 5 CD8a CD8a NNP cord-266018-8bhnlsgy 231 6 À À HYPH cord-266018-8bhnlsgy 231 7 DCs dc NNS cord-266018-8bhnlsgy 231 8 likely likely JJ cord-266018-8bhnlsgy 231 9 function function VBP cord-266018-8bhnlsgy 231 10 in in IN cord-266018-8bhnlsgy 231 11 an an DT cord-266018-8bhnlsgy 231 12 APC APC NNP cord-266018-8bhnlsgy 231 13 - - HYPH cord-266018-8bhnlsgy 231 14 like like JJ cord-266018-8bhnlsgy 231 15 role role NN cord-266018-8bhnlsgy 231 16 within within IN cord-266018-8bhnlsgy 231 17 the the DT cord-266018-8bhnlsgy 231 18 CLN cln NN cord-266018-8bhnlsgy 231 19 following follow VBG cord-266018-8bhnlsgy 231 20 MHV MHV NNP cord-266018-8bhnlsgy 231 21 infection infection NN cord-266018-8bhnlsgy 231 22 and and CC cord-266018-8bhnlsgy 231 23 that that IN cord-266018-8bhnlsgy 231 24 these these DT cord-266018-8bhnlsgy 231 25 cells cell NNS cord-266018-8bhnlsgy 231 26 rely rely VBP cord-266018-8bhnlsgy 231 27 upon upon IN cord-266018-8bhnlsgy 231 28 chemokine chemokine NN cord-266018-8bhnlsgy 231 29 instruction instruction NN cord-266018-8bhnlsgy 231 30 to to TO cord-266018-8bhnlsgy 231 31 activate activate VB cord-266018-8bhnlsgy 231 32 T T NNP cord-266018-8bhnlsgy 231 33 cells cell NNS cord-266018-8bhnlsgy 231 34 . . . cord-266018-8bhnlsgy 232 1 At at IN cord-266018-8bhnlsgy 232 2 a a DT cord-266018-8bhnlsgy 232 3 more more RBR cord-266018-8bhnlsgy 232 4 fundamental fundamental JJ cord-266018-8bhnlsgy 232 5 level level NN cord-266018-8bhnlsgy 232 6 , , , cord-266018-8bhnlsgy 232 7 the the DT cord-266018-8bhnlsgy 232 8 results result NNS cord-266018-8bhnlsgy 232 9 presented present VBD cord-266018-8bhnlsgy 232 10 demonstrate demonstrate VBP cord-266018-8bhnlsgy 232 11 that that IN cord-266018-8bhnlsgy 232 12 chemokines chemokine NNS cord-266018-8bhnlsgy 232 13 serve serve VBP cord-266018-8bhnlsgy 232 14 as as IN cord-266018-8bhnlsgy 232 15 critical critical JJ cord-266018-8bhnlsgy 232 16 upstream upstream NN cord-266018-8bhnlsgy 232 17 signals signal NNS cord-266018-8bhnlsgy 232 18 in in IN cord-266018-8bhnlsgy 232 19 the the DT cord-266018-8bhnlsgy 232 20 innate innate JJ cord-266018-8bhnlsgy 232 21 immune immune JJ cord-266018-8bhnlsgy 232 22 response response NN cord-266018-8bhnlsgy 232 23 that that WDT cord-266018-8bhnlsgy 232 24 later later RB cord-266018-8bhnlsgy 232 25 is be VBZ cord-266018-8bhnlsgy 232 26 important important JJ cord-266018-8bhnlsgy 232 27 with with IN cord-266018-8bhnlsgy 232 28 regards regard NNS cord-266018-8bhnlsgy 232 29 to to IN cord-266018-8bhnlsgy 232 30 the the DT cord-266018-8bhnlsgy 232 31 initiation initiation NN cord-266018-8bhnlsgy 232 32 of of IN cord-266018-8bhnlsgy 232 33 a a DT cord-266018-8bhnlsgy 232 34 protective protective JJ cord-266018-8bhnlsgy 232 35 adaptive adaptive JJ cord-266018-8bhnlsgy 232 36 immune immune JJ cord-266018-8bhnlsgy 232 37 responses response NNS cord-266018-8bhnlsgy 232 38 to to IN cord-266018-8bhnlsgy 232 39 viral viral JJ cord-266018-8bhnlsgy 232 40 infection infection NN cord-266018-8bhnlsgy 232 41 . . . cord-266018-8bhnlsgy 233 1 MHV MHV NNP cord-266018-8bhnlsgy 233 2 J2.2V-1 J2.2V-1 NNP cord-266018-8bhnlsgy 233 3 was be VBD cord-266018-8bhnlsgy 233 4 kindly kindly RB cord-266018-8bhnlsgy 233 5 provided provide VBN cord-266018-8bhnlsgy 233 6 by by IN cord-266018-8bhnlsgy 233 7 J. J. NNP cord-266018-8bhnlsgy 233 8 Fleming Fleming NNP cord-266018-8bhnlsgy 233 9 ( ( -LRB- cord-266018-8bhnlsgy 233 10 U. U. NNP cord-266018-8bhnlsgy 233 11 Wisconsin Wisconsin NNP cord-266018-8bhnlsgy 233 12 ) ) -RRB- cord-266018-8bhnlsgy 233 13 . . . cord-266018-8bhnlsgy 234 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 234 2 À à NN cord-266018-8bhnlsgy 234 3 / / SYM cord-266018-8bhnlsgy 234 4 À à NN cord-266018-8bhnlsgy 234 5 and and CC cord-266018-8bhnlsgy 234 6 CCL3 CCL3 NNP cord-266018-8bhnlsgy 235 1 + + CC cord-266018-8bhnlsgy 235 2 /+ /+ NFP cord-266018-8bhnlsgy 235 3 mice mouse NNS cord-266018-8bhnlsgy 235 4 ( ( -LRB- cord-266018-8bhnlsgy 235 5 C57BL/6 C57BL/6 NNP cord-266018-8bhnlsgy 235 6 , , , cord-266018-8bhnlsgy 235 7 H-2 H-2 NNP cord-266018-8bhnlsgy 235 8 b b NN cord-266018-8bhnlsgy 235 9 ) ) -RRB- cord-266018-8bhnlsgy 235 10 were be VBD cord-266018-8bhnlsgy 235 11 purchased purchase VBN cord-266018-8bhnlsgy 235 12 from from IN cord-266018-8bhnlsgy 235 13 Jackson Jackson NNP cord-266018-8bhnlsgy 235 14 Laboratories Laboratories NNP cord-266018-8bhnlsgy 235 15 ( ( -LRB- cord-266018-8bhnlsgy 235 16 Bar Bar NNP cord-266018-8bhnlsgy 235 17 Harbor Harbor NNP cord-266018-8bhnlsgy 235 18 , , , cord-266018-8bhnlsgy 235 19 ME ME NNP cord-266018-8bhnlsgy 235 20 ) ) -RRB- cord-266018-8bhnlsgy 235 21 . . . cord-266018-8bhnlsgy 236 1 Mice mouse NNS cord-266018-8bhnlsgy 236 2 were be VBD cord-266018-8bhnlsgy 236 3 anesthetized anesthetize VBN cord-266018-8bhnlsgy 236 4 by by IN cord-266018-8bhnlsgy 236 5 inhalation inhalation NN cord-266018-8bhnlsgy 236 6 of of IN cord-266018-8bhnlsgy 236 7 methoxyflurane methoxyflurane NNP cord-266018-8bhnlsgy 237 1 ( ( -LRB- cord-266018-8bhnlsgy 237 2 Pitman Pitman NNP cord-266018-8bhnlsgy 237 3 - - HYPH cord-266018-8bhnlsgy 237 4 Moor Moor NNP cord-266018-8bhnlsgy 237 5 Inc. Inc. NNP cord-266018-8bhnlsgy 237 6 , , , cord-266018-8bhnlsgy 237 7 Washington Washington NNP cord-266018-8bhnlsgy 237 8 Crossing Crossing NNP cord-266018-8bhnlsgy 237 9 , , , cord-266018-8bhnlsgy 237 10 NJ NJ NNP cord-266018-8bhnlsgy 237 11 ) ) -RRB- cord-266018-8bhnlsgy 237 12 and and CC cord-266018-8bhnlsgy 237 13 injected inject VBN cord-266018-8bhnlsgy 237 14 intracranially intracranially RB cord-266018-8bhnlsgy 237 15 ( ( -LRB- cord-266018-8bhnlsgy 237 16 i.c i.c NNP cord-266018-8bhnlsgy 237 17 . . NNP cord-266018-8bhnlsgy 237 18 ) ) -RRB- cord-266018-8bhnlsgy 238 1 with with IN cord-266018-8bhnlsgy 238 2 1000 1000 CD cord-266018-8bhnlsgy 238 3 PFU PFU NNP cord-266018-8bhnlsgy 238 4 MHVJ2.2V-1 MHVJ2.2V-1 NNP cord-266018-8bhnlsgy 238 5 suspended suspend VBN cord-266018-8bhnlsgy 238 6 in in IN cord-266018-8bhnlsgy 238 7 30 30 CD cord-266018-8bhnlsgy 238 8 Al Al NNP cord-266018-8bhnlsgy 238 9 sterile sterile JJ cord-266018-8bhnlsgy 238 10 PBS PBS NNP cord-266018-8bhnlsgy 238 11 . . . cord-266018-8bhnlsgy 239 1 Control Control NNP cord-266018-8bhnlsgy 239 2 ( ( -LRB- cord-266018-8bhnlsgy 239 3 sham sham NN cord-266018-8bhnlsgy 239 4 ) ) -RRB- cord-266018-8bhnlsgy 239 5 mice mouse NNS cord-266018-8bhnlsgy 239 6 were be VBD cord-266018-8bhnlsgy 239 7 injected inject VBN cord-266018-8bhnlsgy 239 8 with with IN cord-266018-8bhnlsgy 239 9 30 30 CD cord-266018-8bhnlsgy 239 10 Al Al NNP cord-266018-8bhnlsgy 239 11 sterile sterile JJ cord-266018-8bhnlsgy 239 12 PBS PBS NNP cord-266018-8bhnlsgy 239 13 alone alone JJ cord-266018-8bhnlsgy 239 14 . . . cord-266018-8bhnlsgy 240 1 Mononuclear Mononuclear NNP cord-266018-8bhnlsgy 240 2 cells cell NNS cord-266018-8bhnlsgy 240 3 were be VBD cord-266018-8bhnlsgy 240 4 obtained obtain VBN cord-266018-8bhnlsgy 240 5 from from IN cord-266018-8bhnlsgy 240 6 the the DT cord-266018-8bhnlsgy 240 7 brains brain NNS cord-266018-8bhnlsgy 240 8 and and CC cord-266018-8bhnlsgy 240 9 cervical cervical JJ cord-266018-8bhnlsgy 240 10 lymph lymph NN cord-266018-8bhnlsgy 240 11 nodes node NNS cord-266018-8bhnlsgy 240 12 [ [ -LRB- cord-266018-8bhnlsgy 240 13 two two CD cord-266018-8bhnlsgy 240 14 draining drain VBG cord-266018-8bhnlsgy 240 15 cervical cervical JJ cord-266018-8bhnlsgy 240 16 lymph lymph NN cord-266018-8bhnlsgy 240 17 nodes node NNS cord-266018-8bhnlsgy 240 18 ( ( -LRB- cord-266018-8bhnlsgy 240 19 CLN CLN NNP cord-266018-8bhnlsgy 240 20 ) ) -RRB- cord-266018-8bhnlsgy 240 21 per per IN cord-266018-8bhnlsgy 240 22 mouse mouse NN cord-266018-8bhnlsgy 240 23 ] ] -RRB- cord-266018-8bhnlsgy 240 24 of of IN cord-266018-8bhnlsgy 240 25 either either DT cord-266018-8bhnlsgy 240 26 CCL3 CCL3 NNP cord-266018-8bhnlsgy 240 27 + + CD cord-266018-8bhnlsgy 241 1 /+ /+ NFP cord-266018-8bhnlsgy 241 2 or or CC cord-266018-8bhnlsgy 242 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 242 2 À à NN cord-266018-8bhnlsgy 242 3 / / SYM cord-266018-8bhnlsgy 242 4 À à NN cord-266018-8bhnlsgy 242 5 mice mouse NNS cord-266018-8bhnlsgy 242 6 at at IN cord-266018-8bhnlsgy 242 7 defined define VBN cord-266018-8bhnlsgy 242 8 times times NNP cord-266018-8bhnlsgy 242 9 post post JJ cord-266018-8bhnlsgy 242 10 - - NN cord-266018-8bhnlsgy 242 11 infection infection JJ cord-266018-8bhnlsgy 242 12 ( ( -LRB- cord-266018-8bhnlsgy 242 13 p.i p.i NNP cord-266018-8bhnlsgy 242 14 . . NNP cord-266018-8bhnlsgy 242 15 ) ) -RRB- cord-266018-8bhnlsgy 243 1 using use VBG cord-266018-8bhnlsgy 243 2 a a DT cord-266018-8bhnlsgy 243 3 previously previously RB cord-266018-8bhnlsgy 243 4 described describe VBN cord-266018-8bhnlsgy 243 5 protocol protocol NN cord-266018-8bhnlsgy 243 6 ( ( -LRB- cord-266018-8bhnlsgy 243 7 Trifilo Trifilo NNP cord-266018-8bhnlsgy 243 8 et et FW cord-266018-8bhnlsgy 243 9 al al NNP cord-266018-8bhnlsgy 243 10 . . NNP cord-266018-8bhnlsgy 243 11 , , , cord-266018-8bhnlsgy 243 12 2003 2003 CD cord-266018-8bhnlsgy 243 13 ) ) -RRB- cord-266018-8bhnlsgy 243 14 . . . cord-266018-8bhnlsgy 244 1 Cell cell NN cord-266018-8bhnlsgy 244 2 surface surface NN cord-266018-8bhnlsgy 244 3 expression expression NN cord-266018-8bhnlsgy 244 4 of of IN cord-266018-8bhnlsgy 244 5 phenotypic phenotypic JJ cord-266018-8bhnlsgy 244 6 markers marker NNS cord-266018-8bhnlsgy 244 7 was be VBD cord-266018-8bhnlsgy 244 8 examined examine VBN cord-266018-8bhnlsgy 244 9 using use VBG cord-266018-8bhnlsgy 244 10 the the DT cord-266018-8bhnlsgy 244 11 following follow VBG cord-266018-8bhnlsgy 244 12 reagents reagent NNS cord-266018-8bhnlsgy 244 13 for for IN cord-266018-8bhnlsgy 244 14 flow flow NN cord-266018-8bhnlsgy 244 15 cytometric cytometric NNP cord-266018-8bhnlsgy 244 16 analysis analysis NN cord-266018-8bhnlsgy 244 17 : : : cord-266018-8bhnlsgy 244 18 APCconjugated apcconjugate VBN cord-266018-8bhnlsgy 244 19 rat rat NN cord-266018-8bhnlsgy 244 20 anti anti JJ cord-266018-8bhnlsgy 244 21 - - JJ cord-266018-8bhnlsgy 244 22 mouse mouse JJ cord-266018-8bhnlsgy 244 23 CD8 CD8 NNP cord-266018-8bhnlsgy 244 24 ; ; : cord-266018-8bhnlsgy 244 25 PERCP PERCP NNP cord-266018-8bhnlsgy 244 26 - - HYPH cord-266018-8bhnlsgy 244 27 conjugated conjugate VBN cord-266018-8bhnlsgy 244 28 rat rat NN cord-266018-8bhnlsgy 244 29 anti anti JJ cord-266018-8bhnlsgy 244 30 mouse mouse NN cord-266018-8bhnlsgy 244 31 CD4 CD4 NNP cord-266018-8bhnlsgy 244 32 ( ( -LRB- cord-266018-8bhnlsgy 244 33 Pharmingen Pharmingen NNP cord-266018-8bhnlsgy 244 34 , , , cord-266018-8bhnlsgy 244 35 San San NNP cord-266018-8bhnlsgy 244 36 Diego Diego NNP cord-266018-8bhnlsgy 244 37 , , , cord-266018-8bhnlsgy 244 38 CA CA NNP cord-266018-8bhnlsgy 244 39 ) ) -RRB- cord-266018-8bhnlsgy 244 40 . . . cord-266018-8bhnlsgy 245 1 PE PE NNP cord-266018-8bhnlsgy 245 2 conjugated conjugate VBN cord-266018-8bhnlsgy 245 3 D d NN cord-266018-8bhnlsgy 245 4 b b NN cord-266018-8bhnlsgy 245 5 /S510 /S510 NNP cord-266018-8bhnlsgy 245 6 - - : cord-266018-8bhnlsgy 245 7 518 518 CD cord-266018-8bhnlsgy 245 8 MHC MHC NNP cord-266018-8bhnlsgy 245 9 class class NN cord-266018-8bhnlsgy 246 1 I -PRON- PRP cord-266018-8bhnlsgy 246 2 tetramer tetramer NNP cord-266018-8bhnlsgy 247 1 ( ( -LRB- cord-266018-8bhnlsgy 247 2 Beckman Beckman NNP cord-266018-8bhnlsgy 247 3 Coulter Coulter NNP cord-266018-8bhnlsgy 247 4 , , , cord-266018-8bhnlsgy 247 5 San San NNP cord-266018-8bhnlsgy 247 6 Diego Diego NNP cord-266018-8bhnlsgy 247 7 , , , cord-266018-8bhnlsgy 247 8 CA CA NNP cord-266018-8bhnlsgy 247 9 ) ) -RRB- cord-266018-8bhnlsgy 247 10 was be VBD cord-266018-8bhnlsgy 247 11 utilized utilize VBN cord-266018-8bhnlsgy 247 12 for for IN cord-266018-8bhnlsgy 247 13 identification identification NN cord-266018-8bhnlsgy 247 14 of of IN cord-266018-8bhnlsgy 247 15 CD8 CD8 NNP cord-266018-8bhnlsgy 247 16 + + CC cord-266018-8bhnlsgy 247 17 T T NNP cord-266018-8bhnlsgy 247 18 cells cell NNS cord-266018-8bhnlsgy 247 19 specific specific JJ cord-266018-8bhnlsgy 247 20 for for IN cord-266018-8bhnlsgy 247 21 viral viral JJ cord-266018-8bhnlsgy 247 22 spike spike NN cord-266018-8bhnlsgy 247 23 protein protein NN cord-266018-8bhnlsgy 247 24 antigen antigen NN cord-266018-8bhnlsgy 247 25 ( ( -LRB- cord-266018-8bhnlsgy 247 26 Trifilo Trifilo NNP cord-266018-8bhnlsgy 247 27 et et FW cord-266018-8bhnlsgy 247 28 al al NNP cord-266018-8bhnlsgy 247 29 . . NNP cord-266018-8bhnlsgy 247 30 , , , cord-266018-8bhnlsgy 247 31 2003 2003 CD cord-266018-8bhnlsgy 247 32 ) ) -RRB- cord-266018-8bhnlsgy 247 33 . . . cord-266018-8bhnlsgy 248 1 To to TO cord-266018-8bhnlsgy 248 2 determine determine VB cord-266018-8bhnlsgy 248 3 the the DT cord-266018-8bhnlsgy 248 4 presence presence NN cord-266018-8bhnlsgy 248 5 of of IN cord-266018-8bhnlsgy 248 6 dendritic dendritic NN cord-266018-8bhnlsgy 248 7 - - HYPH cord-266018-8bhnlsgy 248 8 like like JJ cord-266018-8bhnlsgy 248 9 cells cell NNS cord-266018-8bhnlsgy 248 10 within within IN cord-266018-8bhnlsgy 248 11 the the DT cord-266018-8bhnlsgy 248 12 CNS CNS NNP cord-266018-8bhnlsgy 248 13 and and CC cord-266018-8bhnlsgy 248 14 lymph lymph NN cord-266018-8bhnlsgy 248 15 nodes node NNS cord-266018-8bhnlsgy 248 16 , , , cord-266018-8bhnlsgy 248 17 cells cell NNS cord-266018-8bhnlsgy 248 18 were be VBD cord-266018-8bhnlsgy 248 19 stained stain VBN cord-266018-8bhnlsgy 248 20 using use VBG cord-266018-8bhnlsgy 248 21 FITCconjugated fitcconjugated JJ cord-266018-8bhnlsgy 248 22 rat rat NNP cord-266018-8bhnlsgy 248 23 anti anti JJ cord-266018-8bhnlsgy 248 24 - - JJ cord-266018-8bhnlsgy 248 25 mouse mouse NN cord-266018-8bhnlsgy 248 26 CD11c CD11c NNP cord-266018-8bhnlsgy 248 27 ( ( -LRB- cord-266018-8bhnlsgy 248 28 Serotec Serotec NNP cord-266018-8bhnlsgy 248 29 , , , cord-266018-8bhnlsgy 248 30 Oxford Oxford NNP cord-266018-8bhnlsgy 248 31 , , , cord-266018-8bhnlsgy 248 32 England England NNP cord-266018-8bhnlsgy 248 33 ) ) -RRB- cord-266018-8bhnlsgy 248 34 in in IN cord-266018-8bhnlsgy 248 35 combination combination NN cord-266018-8bhnlsgy 248 36 with with IN cord-266018-8bhnlsgy 248 37 PERCP PERCP NNP cord-266018-8bhnlsgy 248 38 - - HYPH cord-266018-8bhnlsgy 248 39 conjugated conjugate VBN cord-266018-8bhnlsgy 248 40 rat rat NN cord-266018-8bhnlsgy 248 41 anti anti JJ cord-266018-8bhnlsgy 248 42 - - JJ cord-266018-8bhnlsgy 248 43 mouse mouse JJ cord-266018-8bhnlsgy 248 44 CD8a CD8a NNP cord-266018-8bhnlsgy 248 45 ( ( -LRB- cord-266018-8bhnlsgy 248 46 Pharmingen Pharmingen NNP cord-266018-8bhnlsgy 248 47 ) ) -RRB- cord-266018-8bhnlsgy 248 48 , , , cord-266018-8bhnlsgy 248 49 rat rat NN cord-266018-8bhnlsgy 248 50 anti anti JJ cord-266018-8bhnlsgy 248 51 - - JJ cord-266018-8bhnlsgy 248 52 mouse mouse JJ cord-266018-8bhnlsgy 248 53 APC APC NNP cord-266018-8bhnlsgy 248 54 - - HYPH cord-266018-8bhnlsgy 248 55 conjugated conjugate VBN cord-266018-8bhnlsgy 248 56 CD11b CD11b NNS cord-266018-8bhnlsgy 248 57 ( ( -LRB- cord-266018-8bhnlsgy 248 58 Pharmingen Pharmingen NNP cord-266018-8bhnlsgy 248 59 ) ) -RRB- cord-266018-8bhnlsgy 248 60 , , , cord-266018-8bhnlsgy 248 61 and and CC cord-266018-8bhnlsgy 248 62 rat rat NN cord-266018-8bhnlsgy 248 63 anti anti JJ cord-266018-8bhnlsgy 248 64 - - JJ cord-266018-8bhnlsgy 248 65 mouse mouse NN cord-266018-8bhnlsgy 248 66 DEC205 DEC205 NNP cord-266018-8bhnlsgy 248 67 ( ( -LRB- cord-266018-8bhnlsgy 248 68 Pharmingen Pharmingen NNP cord-266018-8bhnlsgy 248 69 ) ) -RRB- cord-266018-8bhnlsgy 248 70 . . . cord-266018-8bhnlsgy 249 1 The the DT cord-266018-8bhnlsgy 249 2 maturation maturation NN cord-266018-8bhnlsgy 249 3 and and CC cord-266018-8bhnlsgy 249 4 activation activation NN cord-266018-8bhnlsgy 249 5 state state NN cord-266018-8bhnlsgy 249 6 of of IN cord-266018-8bhnlsgy 249 7 DC DC NNP cord-266018-8bhnlsgy 249 8 were be VBD cord-266018-8bhnlsgy 249 9 determined determine VBN cord-266018-8bhnlsgy 249 10 using use VBG cord-266018-8bhnlsgy 249 11 FITC FITC NNP cord-266018-8bhnlsgy 249 12 - - HYPH cord-266018-8bhnlsgy 249 13 conjugated conjugate VBN cord-266018-8bhnlsgy 249 14 rat rat NN cord-266018-8bhnlsgy 249 15 anti anti JJ cord-266018-8bhnlsgy 249 16 - - JJ cord-266018-8bhnlsgy 249 17 mouse mouse NN cord-266018-8bhnlsgy 250 1 CD40 CD40 NNP cord-266018-8bhnlsgy 250 2 , , , cord-266018-8bhnlsgy 250 3 CD80 CD80 NNP cord-266018-8bhnlsgy 250 4 , , , cord-266018-8bhnlsgy 250 5 CD86 CD86 NNP cord-266018-8bhnlsgy 250 6 , , , cord-266018-8bhnlsgy 250 7 MHC MHC NNP cord-266018-8bhnlsgy 250 8 I I NNP cord-266018-8bhnlsgy 250 9 , , , cord-266018-8bhnlsgy 250 10 and and CC cord-266018-8bhnlsgy 250 11 MHC MHC NNP cord-266018-8bhnlsgy 250 12 II II NNP cord-266018-8bhnlsgy 250 13 ( ( -LRB- cord-266018-8bhnlsgy 250 14 Pharmingen Pharmingen NNP cord-266018-8bhnlsgy 250 15 ) ) -RRB- cord-266018-8bhnlsgy 250 16 . . . cord-266018-8bhnlsgy 251 1 Isotypematched Isotypematched NNP cord-266018-8bhnlsgy 251 2 antibodies antibody NNS cord-266018-8bhnlsgy 251 3 were be VBD cord-266018-8bhnlsgy 251 4 used use VBN cord-266018-8bhnlsgy 251 5 as as IN cord-266018-8bhnlsgy 251 6 controls control NNS cord-266018-8bhnlsgy 251 7 for for IN cord-266018-8bhnlsgy 251 8 all all DT cord-266018-8bhnlsgy 251 9 staining staining NN cord-266018-8bhnlsgy 251 10 conditions condition NNS cord-266018-8bhnlsgy 251 11 described describe VBN cord-266018-8bhnlsgy 251 12 . . . cord-266018-8bhnlsgy 252 1 Isolation isolation NN cord-266018-8bhnlsgy 252 2 of of IN cord-266018-8bhnlsgy 252 3 CD11c CD11c NNP cord-266018-8bhnlsgy 252 4 + + CC cord-266018-8bhnlsgy 252 5 cells cell NNS cord-266018-8bhnlsgy 252 6 from from IN cord-266018-8bhnlsgy 252 7 the the DT cord-266018-8bhnlsgy 252 8 CLN CLN NNP cord-266018-8bhnlsgy 252 9 CCL3 CCL3 NNP cord-266018-8bhnlsgy 252 10 + + CC cord-266018-8bhnlsgy 252 11 /+ /+ . cord-266018-8bhnlsgy 253 1 and and CC cord-266018-8bhnlsgy 253 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 253 3 À À NNP cord-266018-8bhnlsgy 253 4 / / SYM cord-266018-8bhnlsgy 253 5 À à NN cord-266018-8bhnlsgy 253 6 mice mouse NNS cord-266018-8bhnlsgy 253 7 were be VBD cord-266018-8bhnlsgy 253 8 infected infect VBN cord-266018-8bhnlsgy 253 9 intracranially intracranially RB cord-266018-8bhnlsgy 253 10 with with IN cord-266018-8bhnlsgy 253 11 1000 1000 CD cord-266018-8bhnlsgy 253 12 PFU pfu NN cord-266018-8bhnlsgy 253 13 of of IN cord-266018-8bhnlsgy 253 14 MHV mhv NN cord-266018-8bhnlsgy 253 15 and and CC cord-266018-8bhnlsgy 253 16 brains brain NNS cord-266018-8bhnlsgy 253 17 and and CC cord-266018-8bhnlsgy 253 18 CLN CLN NNP cord-266018-8bhnlsgy 253 19 were be VBD cord-266018-8bhnlsgy 253 20 removed remove VBN cord-266018-8bhnlsgy 253 21 at at IN cord-266018-8bhnlsgy 253 22 defined define VBN cord-266018-8bhnlsgy 253 23 times times IN cord-266018-8bhnlsgy 253 24 post post NN cord-266018-8bhnlsgy 253 25 - - NN cord-266018-8bhnlsgy 253 26 infection infection NN cord-266018-8bhnlsgy 253 27 for for IN cord-266018-8bhnlsgy 253 28 analysis analysis NN cord-266018-8bhnlsgy 253 29 . . . cord-266018-8bhnlsgy 254 1 Brain brain NN cord-266018-8bhnlsgy 254 2 samples sample NNS cord-266018-8bhnlsgy 254 3 were be VBD cord-266018-8bhnlsgy 254 4 minced mince VBN cord-266018-8bhnlsgy 254 5 and and CC cord-266018-8bhnlsgy 254 6 homogenized homogenize VBN cord-266018-8bhnlsgy 254 7 into into IN cord-266018-8bhnlsgy 254 8 a a DT cord-266018-8bhnlsgy 254 9 single single JJ cord-266018-8bhnlsgy 254 10 cell cell NN cord-266018-8bhnlsgy 254 11 suspension suspension NN cord-266018-8bhnlsgy 254 12 followed follow VBN cord-266018-8bhnlsgy 254 13 by by IN cord-266018-8bhnlsgy 254 14 fractionation fractionation NN cord-266018-8bhnlsgy 254 15 on on IN cord-266018-8bhnlsgy 254 16 a a DT cord-266018-8bhnlsgy 254 17 70/30 70/30 CD cord-266018-8bhnlsgy 254 18 % % NN cord-266018-8bhnlsgy 254 19 Percoll Percoll NNP cord-266018-8bhnlsgy 254 20 gradient gradient NN cord-266018-8bhnlsgy 254 21 at at IN cord-266018-8bhnlsgy 254 22 1300 1300 CD cord-266018-8bhnlsgy 254 23 Â â NN cord-266018-8bhnlsgy 254 24 g g NN cord-266018-8bhnlsgy 254 25 for for IN cord-266018-8bhnlsgy 254 26 30 30 CD cord-266018-8bhnlsgy 254 27 min min NN cord-266018-8bhnlsgy 254 28 . . . cord-266018-8bhnlsgy 255 1 For for IN cord-266018-8bhnlsgy 255 2 isolation isolation NN cord-266018-8bhnlsgy 255 3 of of IN cord-266018-8bhnlsgy 255 4 mononuclear mononuclear JJ cord-266018-8bhnlsgy 255 5 cells cell NNS cord-266018-8bhnlsgy 255 6 from from IN cord-266018-8bhnlsgy 255 7 the the DT cord-266018-8bhnlsgy 255 8 CLN cln NN cord-266018-8bhnlsgy 255 9 , , , cord-266018-8bhnlsgy 255 10 lymph lymph NN cord-266018-8bhnlsgy 255 11 nodes node NNS cord-266018-8bhnlsgy 255 12 were be VBD cord-266018-8bhnlsgy 255 13 homogenized homogenize VBN cord-266018-8bhnlsgy 255 14 using use VBG cord-266018-8bhnlsgy 255 15 frosted frosted JJ cord-266018-8bhnlsgy 255 16 glass glass NN cord-266018-8bhnlsgy 255 17 slides slide NNS cord-266018-8bhnlsgy 255 18 and and CC cord-266018-8bhnlsgy 255 19 the the DT cord-266018-8bhnlsgy 255 20 resulting result VBG cord-266018-8bhnlsgy 255 21 single single JJ cord-266018-8bhnlsgy 255 22 cell cell NN cord-266018-8bhnlsgy 255 23 suspension suspension NN cord-266018-8bhnlsgy 255 24 was be VBD cord-266018-8bhnlsgy 255 25 treated treat VBN cord-266018-8bhnlsgy 255 26 with with IN cord-266018-8bhnlsgy 255 27 sterile sterile JJ cord-266018-8bhnlsgy 255 28 H h NN cord-266018-8bhnlsgy 255 29 2 2 CD cord-266018-8bhnlsgy 255 30 O o NN cord-266018-8bhnlsgy 255 31 to to TO cord-266018-8bhnlsgy 255 32 lyse lyse VB cord-266018-8bhnlsgy 255 33 red red JJ cord-266018-8bhnlsgy 255 34 blood blood NN cord-266018-8bhnlsgy 255 35 cells cell NNS cord-266018-8bhnlsgy 255 36 . . . cord-266018-8bhnlsgy 256 1 Due due IN cord-266018-8bhnlsgy 256 2 to to IN cord-266018-8bhnlsgy 256 3 the the DT cord-266018-8bhnlsgy 256 4 low low JJ cord-266018-8bhnlsgy 256 5 frequency frequency NN cord-266018-8bhnlsgy 256 6 and and CC cord-266018-8bhnlsgy 256 7 numbers number NNS cord-266018-8bhnlsgy 256 8 of of IN cord-266018-8bhnlsgy 256 9 CD11c CD11c NNP cord-266018-8bhnlsgy 256 10 + + CC cord-266018-8bhnlsgy 256 11 cells cell NNS cord-266018-8bhnlsgy 256 12 within within IN cord-266018-8bhnlsgy 256 13 the the DT cord-266018-8bhnlsgy 256 14 brain brain NN cord-266018-8bhnlsgy 256 15 and and CC cord-266018-8bhnlsgy 256 16 CLN CLN NNP cord-266018-8bhnlsgy 256 17 , , , cord-266018-8bhnlsgy 256 18 samples sample NNS cord-266018-8bhnlsgy 256 19 from from IN cord-266018-8bhnlsgy 256 20 three three CD cord-266018-8bhnlsgy 256 21 to to TO cord-266018-8bhnlsgy 256 22 six six CD cord-266018-8bhnlsgy 256 23 mice mouse NNS cord-266018-8bhnlsgy 256 24 were be VBD cord-266018-8bhnlsgy 256 25 pooled pool VBN cord-266018-8bhnlsgy 256 26 for for IN cord-266018-8bhnlsgy 256 27 each each DT cord-266018-8bhnlsgy 256 28 experiment experiment NN cord-266018-8bhnlsgy 256 29 . . . cord-266018-8bhnlsgy 257 1 To to TO cord-266018-8bhnlsgy 257 2 enrich enrich VB cord-266018-8bhnlsgy 257 3 for for IN cord-266018-8bhnlsgy 257 4 CD11c CD11c NNP cord-266018-8bhnlsgy 257 5 + + SYM cord-266018-8bhnlsgy 257 6 CD11b CD11b NNS cord-266018-8bhnlsgy 257 7 + + CC cord-266018-8bhnlsgy 258 1 CD8a CD8a NNP cord-266018-8bhnlsgy 258 2 À À NNP cord-266018-8bhnlsgy 258 3 DCs dc NNS cord-266018-8bhnlsgy 258 4 , , , cord-266018-8bhnlsgy 258 5 both both CC cord-266018-8bhnlsgy 258 6 brain brain NN cord-266018-8bhnlsgy 258 7 and and CC cord-266018-8bhnlsgy 258 8 CLN cln NN cord-266018-8bhnlsgy 258 9 samples sample NNS cord-266018-8bhnlsgy 258 10 were be VBD cord-266018-8bhnlsgy 258 11 separately separately RB cord-266018-8bhnlsgy 258 12 magnetically magnetically RB cord-266018-8bhnlsgy 258 13 sorted sort VBN cord-266018-8bhnlsgy 258 14 by by IN cord-266018-8bhnlsgy 258 15 negative negative JJ cord-266018-8bhnlsgy 258 16 selection selection NN cord-266018-8bhnlsgy 258 17 against against IN cord-266018-8bhnlsgy 258 18 CD8a CD8a NNP cord-266018-8bhnlsgy 258 19 using use VBG cord-266018-8bhnlsgy 258 20 MACS MACS NNP cord-266018-8bhnlsgy 258 21 microbeads microbead NNS cord-266018-8bhnlsgy 258 22 coated coat VBN cord-266018-8bhnlsgy 258 23 with with IN cord-266018-8bhnlsgy 258 24 anti anti JJ cord-266018-8bhnlsgy 258 25 - - JJ cord-266018-8bhnlsgy 258 26 CD8a cd8a JJ cord-266018-8bhnlsgy 258 27 ( ( -LRB- cord-266018-8bhnlsgy 258 28 Miltenyi Miltenyi NNP cord-266018-8bhnlsgy 258 29 Biotec Biotec NNP cord-266018-8bhnlsgy 258 30 , , , cord-266018-8bhnlsgy 258 31 Auburn Auburn NNP cord-266018-8bhnlsgy 258 32 , , , cord-266018-8bhnlsgy 258 33 CA CA NNP cord-266018-8bhnlsgy 258 34 ) ) -RRB- cord-266018-8bhnlsgy 258 35 according accord VBG cord-266018-8bhnlsgy 258 36 to to IN cord-266018-8bhnlsgy 258 37 the the DT cord-266018-8bhnlsgy 258 38 manufacturer manufacturer NN cord-266018-8bhnlsgy 258 39 's 's POS cord-266018-8bhnlsgy 258 40 instructions instruction NNS cord-266018-8bhnlsgy 258 41 . . . cord-266018-8bhnlsgy 259 1 CD11c CD11c NNP cord-266018-8bhnlsgy 259 2 + + CC cord-266018-8bhnlsgy 259 3 cells cell NNS cord-266018-8bhnlsgy 259 4 were be VBD cord-266018-8bhnlsgy 259 5 then then RB cord-266018-8bhnlsgy 259 6 magnetically magnetically RB cord-266018-8bhnlsgy 259 7 selected select VBN cord-266018-8bhnlsgy 259 8 from from IN cord-266018-8bhnlsgy 259 9 the the DT cord-266018-8bhnlsgy 259 10 CD8a CD8a NNP cord-266018-8bhnlsgy 259 11 À à NN cord-266018-8bhnlsgy 259 12 fraction fraction NN cord-266018-8bhnlsgy 259 13 using use VBG cord-266018-8bhnlsgy 259 14 MACS MACS NNP cord-266018-8bhnlsgy 259 15 microbeads microbead NNS cord-266018-8bhnlsgy 259 16 coated coat VBN cord-266018-8bhnlsgy 259 17 with with IN cord-266018-8bhnlsgy 259 18 anti anti JJ cord-266018-8bhnlsgy 259 19 - - JJ cord-266018-8bhnlsgy 259 20 CD11c CD11c NNP cord-266018-8bhnlsgy 259 21 ( ( -LRB- cord-266018-8bhnlsgy 259 22 Miltenyi Miltenyi NNP cord-266018-8bhnlsgy 259 23 ) ) -RRB- cord-266018-8bhnlsgy 259 24 . . . cord-266018-8bhnlsgy 260 1 The the DT cord-266018-8bhnlsgy 260 2 resulting result VBG cord-266018-8bhnlsgy 260 3 population population NN cord-266018-8bhnlsgy 260 4 was be VBD cord-266018-8bhnlsgy 260 5 N90 N90 NNP cord-266018-8bhnlsgy 260 6 % % NN cord-266018-8bhnlsgy 260 7 pure pure JJ cord-266018-8bhnlsgy 260 8 for for IN cord-266018-8bhnlsgy 260 9 CD11c CD11c NNP cord-266018-8bhnlsgy 260 10 + + CC cord-266018-8bhnlsgy 260 11 cells cell NNS cord-266018-8bhnlsgy 260 12 that that WDT cord-266018-8bhnlsgy 260 13 were be VBD cord-266018-8bhnlsgy 260 14 subsequently subsequently RB cord-266018-8bhnlsgy 260 15 determined determine VBN cord-266018-8bhnlsgy 260 16 to to TO cord-266018-8bhnlsgy 260 17 be be VB cord-266018-8bhnlsgy 260 18 CD11b CD11b NNS cord-266018-8bhnlsgy 260 19 + + CC cord-266018-8bhnlsgy 260 20 and and CC cord-266018-8bhnlsgy 260 21 CD8a CD8a NNP cord-266018-8bhnlsgy 260 22 À À NNP cord-266018-8bhnlsgy 260 23 by by IN cord-266018-8bhnlsgy 260 24 flow flow NN cord-266018-8bhnlsgy 260 25 cytometry cytometry NN cord-266018-8bhnlsgy 260 26 ( ( -LRB- cord-266018-8bhnlsgy 260 27 data datum NNS cord-266018-8bhnlsgy 260 28 not not RB cord-266018-8bhnlsgy 260 29 shown show VBN cord-266018-8bhnlsgy 260 30 ) ) -RRB- cord-266018-8bhnlsgy 260 31 . . . cord-266018-8bhnlsgy 261 1 MACS mac NNS cord-266018-8bhnlsgy 261 2 enriched enrich VBD cord-266018-8bhnlsgy 261 3 cells cell NNS cord-266018-8bhnlsgy 261 4 were be VBD cord-266018-8bhnlsgy 261 5 then then RB cord-266018-8bhnlsgy 261 6 resuspended resuspend VBN cord-266018-8bhnlsgy 261 7 in in IN cord-266018-8bhnlsgy 261 8 DMEM DMEM NNP cord-266018-8bhnlsgy 261 9 supplemented supplement VBD cord-266018-8bhnlsgy 261 10 with with IN cord-266018-8bhnlsgy 261 11 10 10 CD cord-266018-8bhnlsgy 261 12 % % NN cord-266018-8bhnlsgy 261 13 FBS FBS NNP cord-266018-8bhnlsgy 261 14 . . . cord-266018-8bhnlsgy 262 1 Cytokine cytokine NN cord-266018-8bhnlsgy 262 2 and and CC cord-266018-8bhnlsgy 262 3 chemokine chemokine JJ cord-266018-8bhnlsgy 262 4 expression expression NN cord-266018-8bhnlsgy 262 5 from from IN cord-266018-8bhnlsgy 262 6 CD11c CD11c NNP cord-266018-8bhnlsgy 262 7 + + CC cord-266018-8bhnlsgy 262 8 CD8a CD8a NNP cord-266018-8bhnlsgy 262 9 À à NN cord-266018-8bhnlsgy 262 10 cells cell NNS cord-266018-8bhnlsgy 263 1 Freshly freshly RB cord-266018-8bhnlsgy 263 2 prepared prepared JJ cord-266018-8bhnlsgy 264 1 CD11c CD11c NNP cord-266018-8bhnlsgy 264 2 + + CC cord-266018-8bhnlsgy 264 3 CD8a CD8a NNP cord-266018-8bhnlsgy 264 4 À à NN cord-266018-8bhnlsgy 264 5 cells cell NNS cord-266018-8bhnlsgy 264 6 were be VBD cord-266018-8bhnlsgy 264 7 isolated isolate VBN cord-266018-8bhnlsgy 264 8 from from IN cord-266018-8bhnlsgy 264 9 the the DT cord-266018-8bhnlsgy 264 10 CLN cln NN cord-266018-8bhnlsgy 264 11 from from IN cord-266018-8bhnlsgy 264 12 CCL3 CCL3 NNP cord-266018-8bhnlsgy 264 13 + + CC cord-266018-8bhnlsgy 265 1 /+ /+ NFP cord-266018-8bhnlsgy 265 2 and and CC cord-266018-8bhnlsgy 266 1 CCL3 CCL3 NNP cord-266018-8bhnlsgy 266 2 À à NN cord-266018-8bhnlsgy 266 3 / / SYM cord-266018-8bhnlsgy 266 4 À à NN cord-266018-8bhnlsgy 266 5 mice mouse NNS cord-266018-8bhnlsgy 266 6 at at IN cord-266018-8bhnlsgy 266 7 day day NN cord-266018-8bhnlsgy 266 8 7 7 CD cord-266018-8bhnlsgy 266 9 p.i p.i NNP cord-266018-8bhnlsgy 266 10 . . . cord-266018-8bhnlsgy 267 1 and and CC cord-266018-8bhnlsgy 267 2 seeded seed VBN cord-266018-8bhnlsgy 267 3 into into IN cord-266018-8bhnlsgy 267 4 96-well 96-well CD cord-266018-8bhnlsgy 267 5 plate plate NN cord-266018-8bhnlsgy 267 6 at at IN cord-266018-8bhnlsgy 267 7 a a DT cord-266018-8bhnlsgy 267 8 cell cell NN cord-266018-8bhnlsgy 267 9 density density NN cord-266018-8bhnlsgy 267 10 of of IN cord-266018-8bhnlsgy 267 11 2 2 CD cord-266018-8bhnlsgy 267 12 Â â CD cord-266018-8bhnlsgy 267 13 10 10 CD cord-266018-8bhnlsgy 267 14 5 5 CD cord-266018-8bhnlsgy 268 1 cells/200 cells/200 NNP cord-266018-8bhnlsgy 268 2 Al Al NNP cord-266018-8bhnlsgy 268 3 in in IN cord-266018-8bhnlsgy 268 4 DMEM DMEM NNP cord-266018-8bhnlsgy 268 5 containing contain VBG cord-266018-8bhnlsgy 268 6 10 10 CD cord-266018-8bhnlsgy 268 7 % % NN cord-266018-8bhnlsgy 268 8 fetal fetal JJ cord-266018-8bhnlsgy 268 9 bovine bovine JJ cord-266018-8bhnlsgy 268 10 serum serum NN cord-266018-8bhnlsgy 268 11 ( ( -LRB- cord-266018-8bhnlsgy 268 12 FBS FBS NNP cord-266018-8bhnlsgy 268 13 , , , cord-266018-8bhnlsgy 268 14 BioWhittaker BioWhittaker NNP cord-266018-8bhnlsgy 268 15 , , , cord-266018-8bhnlsgy 268 16 Walkersville Walkersville NNP cord-266018-8bhnlsgy 268 17 , , , cord-266018-8bhnlsgy 268 18 MD MD NNP cord-266018-8bhnlsgy 268 19 ) ) -RRB- cord-266018-8bhnlsgy 268 20 . . . cord-266018-8bhnlsgy 269 1 Following follow VBG cord-266018-8bhnlsgy 269 2 isola isola NNP cord-266018-8bhnlsgy 269 3 - - HYPH cord-266018-8bhnlsgy 269 4 tion tion NN cord-266018-8bhnlsgy 269 5 , , , cord-266018-8bhnlsgy 269 6 cell cell NN cord-266018-8bhnlsgy 269 7 viability viability NN cord-266018-8bhnlsgy 269 8 was be VBD cord-266018-8bhnlsgy 269 9 N95 N95 NNP cord-266018-8bhnlsgy 269 10 % % NN cord-266018-8bhnlsgy 269 11 . . . cord-266018-8bhnlsgy 270 1 After after IN cord-266018-8bhnlsgy 270 2 24 24 CD cord-266018-8bhnlsgy 270 3 h h NN cord-266018-8bhnlsgy 270 4 , , , cord-266018-8bhnlsgy 270 5 supernatants supernatant NNS cord-266018-8bhnlsgy 270 6 from from IN cord-266018-8bhnlsgy 270 7 the the DT cord-266018-8bhnlsgy 270 8 samples sample NNS cord-266018-8bhnlsgy 270 9 were be VBD cord-266018-8bhnlsgy 270 10 collected collect VBN cord-266018-8bhnlsgy 270 11 , , , cord-266018-8bhnlsgy 270 12 and and CC cord-266018-8bhnlsgy 270 13 the the DT cord-266018-8bhnlsgy 270 14 level level NN cord-266018-8bhnlsgy 270 15 of of IN cord-266018-8bhnlsgy 270 16 IL-12 IL-12 NNP cord-266018-8bhnlsgy 270 17 , , , cord-266018-8bhnlsgy 270 18 IL-10 IL-10 NNP cord-266018-8bhnlsgy 270 19 , , , cord-266018-8bhnlsgy 270 20 and and CC cord-266018-8bhnlsgy 270 21 CCL3 CCL3 NNP cord-266018-8bhnlsgy 270 22 were be VBD cord-266018-8bhnlsgy 270 23 determined determine VBN cord-266018-8bhnlsgy 270 24 using use VBG cord-266018-8bhnlsgy 270 25 Quantikine Quantikine NNP cord-266018-8bhnlsgy 270 26 M M NNP cord-266018-8bhnlsgy 270 27 mouse mouse NN cord-266018-8bhnlsgy 270 28 immunoassays immunoassays NNP cord-266018-8bhnlsgy 270 29 kits kit NNS cord-266018-8bhnlsgy 270 30 ( ( -LRB- cord-266018-8bhnlsgy 270 31 R&D R&D NNP cord-266018-8bhnlsgy 270 32 Systems Systems NNPS cord-266018-8bhnlsgy 270 33 , , , cord-266018-8bhnlsgy 270 34 Minneapolis Minneapolis NNP cord-266018-8bhnlsgy 270 35 , , , cord-266018-8bhnlsgy 270 36 MN MN NNP cord-266018-8bhnlsgy 270 37 ) ) -RRB- cord-266018-8bhnlsgy 270 38 according accord VBG cord-266018-8bhnlsgy 270 39 to to IN cord-266018-8bhnlsgy 270 40 manufacturer manufacturer NN cord-266018-8bhnlsgy 270 41 's 's POS cord-266018-8bhnlsgy 270 42 specifications specification NNS cord-266018-8bhnlsgy 270 43 . . . cord-266018-8bhnlsgy 271 1 Assays assay NNS cord-266018-8bhnlsgy 271 2 had have VBD cord-266018-8bhnlsgy 271 3 a a DT cord-266018-8bhnlsgy 271 4 minimum minimum JJ cord-266018-8bhnlsgy 271 5 sensitivity sensitivity NN cord-266018-8bhnlsgy 271 6 of of IN cord-266018-8bhnlsgy 271 7 5 5 CD cord-266018-8bhnlsgy 271 8 pg pg NN cord-266018-8bhnlsgy 271 9 / / SYM cord-266018-8bhnlsgy 271 10 ml ml NN cord-266018-8bhnlsgy 271 11 ( ( -LRB- cord-266018-8bhnlsgy 271 12 IL-10 IL-10 NNP cord-266018-8bhnlsgy 271 13 and and CC cord-266018-8bhnlsgy 271 14 IL-12p70 IL-12p70 NNP cord-266018-8bhnlsgy 271 15 ) ) -RRB- cord-266018-8bhnlsgy 271 16 and and CC cord-266018-8bhnlsgy 271 17 10 10 CD cord-266018-8bhnlsgy 271 18 pg pg NN cord-266018-8bhnlsgy 271 19 / / SYM cord-266018-8bhnlsgy 271 20 ml ml NNP cord-266018-8bhnlsgy 271 21 ( ( -LRB- cord-266018-8bhnlsgy 271 22 CCL3 CCL3 NNP cord-266018-8bhnlsgy 271 23 ) ) -RRB- cord-266018-8bhnlsgy 271 24 . . . cord-266018-8bhnlsgy 272 1 Mononuclear Mononuclear NNP cord-266018-8bhnlsgy 272 2 cells cell NNS cord-266018-8bhnlsgy 272 3 were be VBD cord-266018-8bhnlsgy 272 4 obtained obtain VBN cord-266018-8bhnlsgy 272 5 from from IN cord-266018-8bhnlsgy 272 6 the the DT cord-266018-8bhnlsgy 272 7 CLNs cln NNS cord-266018-8bhnlsgy 272 8 of of IN cord-266018-8bhnlsgy 272 9 MHV MHV NNP cord-266018-8bhnlsgy 272 10 - - HYPH cord-266018-8bhnlsgy 272 11 infected infect VBN cord-266018-8bhnlsgy 272 12 mice mouse NNS cord-266018-8bhnlsgy 272 13 at at IN cord-266018-8bhnlsgy 272 14 defined define VBN cord-266018-8bhnlsgy 272 15 times times NNP cord-266018-8bhnlsgy 272 16 post post NN cord-266018-8bhnlsgy 272 17 - - NN cord-266018-8bhnlsgy 272 18 infection infection JJ cord-266018-8bhnlsgy 272 19 and and CC cord-266018-8bhnlsgy 272 20 cytokine cytokine NN cord-266018-8bhnlsgy 272 21 production production NN cord-266018-8bhnlsgy 272 22 by by IN cord-266018-8bhnlsgy 272 23 T t NN cord-266018-8bhnlsgy 272 24 cells cell NNS cord-266018-8bhnlsgy 272 25 determined determine VBN cord-266018-8bhnlsgy 272 26 by by IN cord-266018-8bhnlsgy 272 27 intracellular intracellular JJ cord-266018-8bhnlsgy 272 28 cytokine cytokine NN cord-266018-8bhnlsgy 272 29 staining staining NN cord-266018-8bhnlsgy 272 30 to to IN cord-266018-8bhnlsgy 272 31 defined define VBN cord-266018-8bhnlsgy 272 32 viral viral JJ cord-266018-8bhnlsgy 272 33 antigens antigen NNS cord-266018-8bhnlsgy 272 34 using use VBG cord-266018-8bhnlsgy 272 35 a a DT cord-266018-8bhnlsgy 272 36 previously previously RB cord-266018-8bhnlsgy 272 37 described describe VBN cord-266018-8bhnlsgy 272 38 protocol protocol NN cord-266018-8bhnlsgy 272 39 ( ( -LRB- cord-266018-8bhnlsgy 272 40 Trifilo Trifilo NNP cord-266018-8bhnlsgy 272 41 et et FW cord-266018-8bhnlsgy 272 42 al al NNP cord-266018-8bhnlsgy 272 43 . . NNP cord-266018-8bhnlsgy 272 44 , , , cord-266018-8bhnlsgy 272 45 2003 2003 CD cord-266018-8bhnlsgy 272 46 ) ) -RRB- cord-266018-8bhnlsgy 272 47 . . . cord-266018-8bhnlsgy 273 1 Statistically statistically RB cord-266018-8bhnlsgy 273 2 significant significant JJ cord-266018-8bhnlsgy 273 3 differences difference NNS cord-266018-8bhnlsgy 273 4 between between IN cord-266018-8bhnlsgy 273 5 experimental experimental JJ cord-266018-8bhnlsgy 273 6 groups group NNS cord-266018-8bhnlsgy 273 7 was be VBD cord-266018-8bhnlsgy 273 8 determined determine VBN cord-266018-8bhnlsgy 273 9 by by IN cord-266018-8bhnlsgy 273 10 the the DT cord-266018-8bhnlsgy 273 11 Mann Mann NNP cord-266018-8bhnlsgy 273 12 - - HYPH cord-266018-8bhnlsgy 273 13 Whitney Whitney NNP cord-266018-8bhnlsgy 273 14 Rank Rank NNP cord-266018-8bhnlsgy 273 15 Sum Sum NNP cord-266018-8bhnlsgy 273 16 Test Test NNP cord-266018-8bhnlsgy 273 17 , , , cord-266018-8bhnlsgy 273 18 and and CC cord-266018-8bhnlsgy 273 19 P p NN cord-266018-8bhnlsgy 273 20 values value NNS cord-266018-8bhnlsgy 273 21 of of IN cord-266018-8bhnlsgy 273 22 V0.05 V0.05 NNP cord-266018-8bhnlsgy 273 23 were be VBD cord-266018-8bhnlsgy 273 24 considered consider VBN cord-266018-8bhnlsgy 273 25 significant significant JJ cord-266018-8bhnlsgy 273 26 . . . cord-266018-8bhnlsgy 274 1 CCR5 CCR5 NNP cord-266018-8bhnlsgy 274 2 provides provide VBZ cord-266018-8bhnlsgy 274 3 a a DT cord-266018-8bhnlsgy 274 4 signal signal NN cord-266018-8bhnlsgy 274 5 for for IN cord-266018-8bhnlsgy 274 6 microbial microbial JJ cord-266018-8bhnlsgy 274 7 induced induce VBN cord-266018-8bhnlsgy 274 8 production production NN cord-266018-8bhnlsgy 274 9 of of IN cord-266018-8bhnlsgy 274 10 IL-12 IL-12 NNP cord-266018-8bhnlsgy 274 11 by by IN cord-266018-8bhnlsgy 274 12 CD8a+ cd8a+ JJ cord-266018-8bhnlsgy 274 13 dendritic dendritic JJ cord-266018-8bhnlsgy 274 14 cells cell NNS cord-266018-8bhnlsgy 275 1 Definition definition NN cord-266018-8bhnlsgy 275 2 of of IN cord-266018-8bhnlsgy 275 3 dendritic dendritic JJ cord-266018-8bhnlsgy 275 4 cell cell NN cord-266018-8bhnlsgy 275 5 subpopulations subpopulation NNS cord-266018-8bhnlsgy 275 6 present present JJ cord-266018-8bhnlsgy 275 7 in in IN cord-266018-8bhnlsgy 275 8 the the DT cord-266018-8bhnlsgy 275 9 spleen spleen NN cord-266018-8bhnlsgy 275 10 , , , cord-266018-8bhnlsgy 275 11 Peyer Peyer NNP cord-266018-8bhnlsgy 275 12 's 's POS cord-266018-8bhnlsgy 275 13 patches patch NNS cord-266018-8bhnlsgy 275 14 , , , cord-266018-8bhnlsgy 275 15 lymph lymph NN cord-266018-8bhnlsgy 275 16 nodes node NNS cord-266018-8bhnlsgy 275 17 , , , cord-266018-8bhnlsgy 275 18 and and CC cord-266018-8bhnlsgy 275 19 skin skin NN cord-266018-8bhnlsgy 275 20 of of IN cord-266018-8bhnlsgy 275 21 the the DT cord-266018-8bhnlsgy 275 22 mouse mouse NN cord-266018-8bhnlsgy 275 23 Dendritic dendritic JJ cord-266018-8bhnlsgy 275 24 cells cell NNS cord-266018-8bhnlsgy 275 25 and and CC cord-266018-8bhnlsgy 275 26 the the DT cord-266018-8bhnlsgy 275 27 control control NN cord-266018-8bhnlsgy 275 28 of of IN cord-266018-8bhnlsgy 275 29 immunity immunity NN cord-266018-8bhnlsgy 276 1 CD8 CD8 NNP cord-266018-8bhnlsgy 276 2 + + CC cord-266018-8bhnlsgy 276 3 T T NNP cord-266018-8bhnlsgy 276 4 - - HYPH cord-266018-8bhnlsgy 276 5 cell cell NN cord-266018-8bhnlsgy 276 6 epitopes epitope NNS cord-266018-8bhnlsgy 276 7 within within IN cord-266018-8bhnlsgy 276 8 the the DT cord-266018-8bhnlsgy 276 9 surface surface NN cord-266018-8bhnlsgy 276 10 glycoprotein glycoprotein NN cord-266018-8bhnlsgy 276 11 of of IN cord-266018-8bhnlsgy 276 12 a a DT cord-266018-8bhnlsgy 276 13 neurotropic neurotropic JJ cord-266018-8bhnlsgy 276 14 coronavirus coronavirus NN cord-266018-8bhnlsgy 276 15 and and CC cord-266018-8bhnlsgy 276 16 correlation correlation NN cord-266018-8bhnlsgy 276 17 with with IN cord-266018-8bhnlsgy 276 18 pathogenicity pathogenicity NN cord-266018-8bhnlsgy 276 19 Ligation Ligation NNP cord-266018-8bhnlsgy 276 20 of of IN cord-266018-8bhnlsgy 276 21 CD40 CD40 NNP cord-266018-8bhnlsgy 276 22 on on IN cord-266018-8bhnlsgy 276 23 dendritic dendritic JJ cord-266018-8bhnlsgy 276 24 cells cell NNS cord-266018-8bhnlsgy 276 25 triggers trigger VBZ cord-266018-8bhnlsgy 276 26 production production NN cord-266018-8bhnlsgy 276 27 of of IN cord-266018-8bhnlsgy 276 28 high high JJ cord-266018-8bhnlsgy 276 29 levels level NNS cord-266018-8bhnlsgy 276 30 of of IN cord-266018-8bhnlsgy 276 31 interleukin-12 interleukin-12 CD cord-266018-8bhnlsgy 276 32 and and CC cord-266018-8bhnlsgy 276 33 enhances enhance VBZ cord-266018-8bhnlsgy 276 34 T t NN cord-266018-8bhnlsgy 276 35 cell cell NN cord-266018-8bhnlsgy 276 36 stimulatory stimulatory JJ cord-266018-8bhnlsgy 276 37 capacity capacity NN cord-266018-8bhnlsgy 276 38 : : : cord-266018-8bhnlsgy 276 39 T T NNP cord-266018-8bhnlsgy 276 40 - - HYPH cord-266018-8bhnlsgy 276 41 T T NNP cord-266018-8bhnlsgy 276 42 help help NN cord-266018-8bhnlsgy 276 43 via via IN cord-266018-8bhnlsgy 276 44 APC APC NNP cord-266018-8bhnlsgy 276 45 activation activation NN cord-266018-8bhnlsgy 276 46 Lack Lack NNP cord-266018-8bhnlsgy 276 47 of of IN cord-266018-8bhnlsgy 276 48 CCR2 CCR2 NNP cord-266018-8bhnlsgy 276 49 results result NNS cord-266018-8bhnlsgy 276 50 in in IN cord-266018-8bhnlsgy 276 51 increased increase VBN cord-266018-8bhnlsgy 276 52 mortality mortality NN cord-266018-8bhnlsgy 276 53 and and CC cord-266018-8bhnlsgy 276 54 impaired impaired JJ cord-266018-8bhnlsgy 276 55 leukocyte leukocyte NN cord-266018-8bhnlsgy 276 56 activation activation NN cord-266018-8bhnlsgy 276 57 and and CC cord-266018-8bhnlsgy 276 58 trafficking trafficking NN cord-266018-8bhnlsgy 276 59 following follow VBG cord-266018-8bhnlsgy 276 60 infection infection NN cord-266018-8bhnlsgy 276 61 of of IN cord-266018-8bhnlsgy 276 62 the the DT cord-266018-8bhnlsgy 276 63 central central JJ cord-266018-8bhnlsgy 276 64 system system NN cord-266018-8bhnlsgy 276 65 with with IN cord-266018-8bhnlsgy 276 66 a a DT cord-266018-8bhnlsgy 276 67 neurotropic neurotropic NNP cord-266018-8bhnlsgy 276 68 coronavirus coronavirus NN cord-266018-8bhnlsgy 276 69 Requirement Requirement NNP cord-266018-8bhnlsgy 276 70 of of IN cord-266018-8bhnlsgy 276 71 MIP-1 MIP-1 NNP cord-266018-8bhnlsgy 276 72 alpha alpha NN cord-266018-8bhnlsgy 276 73 for for IN cord-266018-8bhnlsgy 276 74 an an DT cord-266018-8bhnlsgy 276 75 inflammatory inflammatory JJ cord-266018-8bhnlsgy 276 76 response response NN cord-266018-8bhnlsgy 276 77 to to IN cord-266018-8bhnlsgy 276 78 viral viral JJ cord-266018-8bhnlsgy 276 79 infection infection NN cord-266018-8bhnlsgy 277 1 The the DT cord-266018-8bhnlsgy 277 2 chemokine chemokine NN cord-266018-8bhnlsgy 277 3 macrophage macrophage NN cord-266018-8bhnlsgy 277 4 - - HYPH cord-266018-8bhnlsgy 277 5 inflammatory inflammatory JJ cord-266018-8bhnlsgy 277 6 protein-1 protein-1 NNP cord-266018-8bhnlsgy 277 7 alpha alpha NN cord-266018-8bhnlsgy 277 8 and and CC cord-266018-8bhnlsgy 277 9 its -PRON- PRP$ cord-266018-8bhnlsgy 277 10 receptor receptor NN cord-266018-8bhnlsgy 277 11 CCR1 CCR1 NNP cord-266018-8bhnlsgy 277 12 control control NN cord-266018-8bhnlsgy 277 13 pulmonary pulmonary JJ cord-266018-8bhnlsgy 277 14 inflammation inflammation NN cord-266018-8bhnlsgy 277 15 and and CC cord-266018-8bhnlsgy 277 16 antiviral antiviral JJ cord-266018-8bhnlsgy 277 17 host host NN cord-266018-8bhnlsgy 277 18 defense defense NN cord-266018-8bhnlsgy 277 19 in in IN cord-266018-8bhnlsgy 277 20 paramyxovirus paramyxovirus JJ cord-266018-8bhnlsgy 277 21 infection infection NN cord-266018-8bhnlsgy 278 1 The the DT cord-266018-8bhnlsgy 278 2 immune immune JJ cord-266018-8bhnlsgy 278 3 response response NN cord-266018-8bhnlsgy 278 4 modifier modifier NN cord-266018-8bhnlsgy 278 5 and and CC cord-266018-8bhnlsgy 278 6 Toll toll NN cord-266018-8bhnlsgy 278 7 - - HYPH cord-266018-8bhnlsgy 278 8 like like JJ cord-266018-8bhnlsgy 278 9 receptor receptor NN cord-266018-8bhnlsgy 278 10 7 7 CD cord-266018-8bhnlsgy 278 11 agonist agonist NN cord-266018-8bhnlsgy 279 1 S-27609 S-27609 NNS cord-266018-8bhnlsgy 279 2 selectively selectively RB cord-266018-8bhnlsgy 279 3 induces induce VBZ cord-266018-8bhnlsgy 279 4 IL-12 IL-12 NNP cord-266018-8bhnlsgy 279 5 and and CC cord-266018-8bhnlsgy 279 6 TNF tnf NN cord-266018-8bhnlsgy 279 7 - - HYPH cord-266018-8bhnlsgy 279 8 alpha alpha NN cord-266018-8bhnlsgy 279 9 production production NN cord-266018-8bhnlsgy 279 10 in in IN cord-266018-8bhnlsgy 279 11 CD11c+CD11b+CD8-dendritic cd11c+cd11b+cd8-dendritic JJ cord-266018-8bhnlsgy 279 12 cells cell NNS cord-266018-8bhnlsgy 279 13 Brain Brain NNP cord-266018-8bhnlsgy 279 14 dendritic dendritic JJ cord-266018-8bhnlsgy 279 15 cells cell NNS cord-266018-8bhnlsgy 279 16 and and CC cord-266018-8bhnlsgy 279 17 macrophages/ macrophages/ NN cord-266018-8bhnlsgy 279 18 microglia microglia NNS cord-266018-8bhnlsgy 279 19 in in IN cord-266018-8bhnlsgy 279 20 central central JJ cord-266018-8bhnlsgy 279 21 nervous nervous JJ cord-266018-8bhnlsgy 279 22 system system NN cord-266018-8bhnlsgy 279 23 inflammation inflammation NN cord-266018-8bhnlsgy 279 24 Phenotype phenotype NN cord-266018-8bhnlsgy 279 25 and and CC cord-266018-8bhnlsgy 279 26 functions function NNS cord-266018-8bhnlsgy 279 27 of of IN cord-266018-8bhnlsgy 279 28 brain brain NN cord-266018-8bhnlsgy 279 29 dendritic dendritic JJ cord-266018-8bhnlsgy 279 30 cells cell NNS cord-266018-8bhnlsgy 279 31 emerging emerge VBG cord-266018-8bhnlsgy 279 32 during during IN cord-266018-8bhnlsgy 279 33 chronic chronic JJ cord-266018-8bhnlsgy 279 34 infection infection NN cord-266018-8bhnlsgy 279 35 of of IN cord-266018-8bhnlsgy 279 36 mice mouse NNS cord-266018-8bhnlsgy 279 37 with with IN cord-266018-8bhnlsgy 279 38 Toxoplasma Toxoplasma NNP cord-266018-8bhnlsgy 279 39 gondii gondii NNP cord-266018-8bhnlsgy 279 40 Reduced reduce VBN cord-266018-8bhnlsgy 279 41 macrophage macrophage NN cord-266018-8bhnlsgy 279 42 infiltration infiltration NN cord-266018-8bhnlsgy 279 43 and and CC cord-266018-8bhnlsgy 279 44 demyelination demyelination NN cord-266018-8bhnlsgy 279 45 in in IN cord-266018-8bhnlsgy 279 46 mice mouse NNS cord-266018-8bhnlsgy 279 47 lacking lack VBG cord-266018-8bhnlsgy 279 48 the the DT cord-266018-8bhnlsgy 279 49 chemokine chemokine NN cord-266018-8bhnlsgy 279 50 receptor receptor NN cord-266018-8bhnlsgy 279 51 CCR5 CCR5 NNP cord-266018-8bhnlsgy 279 52 following follow VBG cord-266018-8bhnlsgy 279 53 infection infection NN cord-266018-8bhnlsgy 279 54 with with IN cord-266018-8bhnlsgy 279 55 a a DT cord-266018-8bhnlsgy 279 56 neurotropic neurotropic JJ cord-266018-8bhnlsgy 279 57 coronavirus coronavirus NN cord-266018-8bhnlsgy 279 58 The the DT cord-266018-8bhnlsgy 279 59 dendritic dendritic JJ cord-266018-8bhnlsgy 279 60 cell cell NN cord-266018-8bhnlsgy 279 61 populations population NNS cord-266018-8bhnlsgy 279 62 of of IN cord-266018-8bhnlsgy 279 63 mouse mouse NN cord-266018-8bhnlsgy 279 64 lymph lymph NN cord-266018-8bhnlsgy 279 65 nodes node NNS cord-266018-8bhnlsgy 280 1 The the DT cord-266018-8bhnlsgy 280 2 T T NNP cord-266018-8bhnlsgy 280 3 cell cell NN cord-266018-8bhnlsgy 280 4 chemoattractant chemoattractant NN cord-266018-8bhnlsgy 281 1 IFNinducible ifninducible JJ cord-266018-8bhnlsgy 281 2 protein protein NN cord-266018-8bhnlsgy 281 3 10 10 CD cord-266018-8bhnlsgy 281 4 is be VBZ cord-266018-8bhnlsgy 281 5 essential essential JJ cord-266018-8bhnlsgy 281 6 in in IN cord-266018-8bhnlsgy 281 7 host host NN cord-266018-8bhnlsgy 281 8 defense defense NN cord-266018-8bhnlsgy 281 9 against against IN cord-266018-8bhnlsgy 281 10 viral viral RB cord-266018-8bhnlsgy 281 11 - - HYPH cord-266018-8bhnlsgy 281 12 induced induce VBN cord-266018-8bhnlsgy 281 13 neurologic neurologic JJ cord-266018-8bhnlsgy 281 14 disease disease NN cord-266018-8bhnlsgy 282 1 The the DT cord-266018-8bhnlsgy 282 2 role role NN cord-266018-8bhnlsgy 282 3 of of IN cord-266018-8bhnlsgy 282 4 chemokines chemokine NNS cord-266018-8bhnlsgy 282 5 in in IN cord-266018-8bhnlsgy 282 6 linking link VBG cord-266018-8bhnlsgy 282 7 innate innate JJ cord-266018-8bhnlsgy 282 8 and and CC cord-266018-8bhnlsgy 282 9 adaptive adaptive JJ cord-266018-8bhnlsgy 282 10 immunity immunity NN cord-266018-8bhnlsgy 282 11 Dendritic dendritic JJ cord-266018-8bhnlsgy 282 12 cells cell NNS cord-266018-8bhnlsgy 282 13 produce produce VBP cord-266018-8bhnlsgy 282 14 IL-12 IL-12 NNP cord-266018-8bhnlsgy 282 15 and and CC cord-266018-8bhnlsgy 282 16 direct direct VB cord-266018-8bhnlsgy 282 17 the the DT cord-266018-8bhnlsgy 282 18 development development NN cord-266018-8bhnlsgy 282 19 of of IN cord-266018-8bhnlsgy 282 20 Th1 th1 NN cord-266018-8bhnlsgy 282 21 cells cell NNS cord-266018-8bhnlsgy 282 22 from from IN cord-266018-8bhnlsgy 282 23 naRve naRve NNP cord-266018-8bhnlsgy 282 24 CD4 CD4 NNP cord-266018-8bhnlsgy 282 25 + + CC cord-266018-8bhnlsgy 282 26 T T NNP cord-266018-8bhnlsgy 282 27 cells cell NNS cord-266018-8bhnlsgy 282 28 CD8alpha(+ CD8alpha(+ NNP cord-266018-8bhnlsgy 282 29 ) ) -RRB- cord-266018-8bhnlsgy 282 30 and and CC cord-266018-8bhnlsgy 283 1 CD8alpha(À cd8alpha(à LS cord-266018-8bhnlsgy 283 2 ) ) -RRB- cord-266018-8bhnlsgy 283 3 subclasses subclass NNS cord-266018-8bhnlsgy 283 4 of of IN cord-266018-8bhnlsgy 283 5 dendritic dendritic JJ cord-266018-8bhnlsgy 283 6 cells cell NNS cord-266018-8bhnlsgy 283 7 direct direct VBP cord-266018-8bhnlsgy 283 8 the the DT cord-266018-8bhnlsgy 283 9 development development NN cord-266018-8bhnlsgy 283 10 of of IN cord-266018-8bhnlsgy 283 11 distinct distinct JJ cord-266018-8bhnlsgy 283 12 T T NNP cord-266018-8bhnlsgy 283 13 helper helper NN cord-266018-8bhnlsgy 283 14 cells cell NNS cord-266018-8bhnlsgy 283 15 in in IN cord-266018-8bhnlsgy 283 16 vivo vivo NNP cord-266018-8bhnlsgy 283 17 Cytokines Cytokines NNP cord-266018-8bhnlsgy 283 18 regulate regulate VBP cord-266018-8bhnlsgy 283 19 the the DT cord-266018-8bhnlsgy 283 20 capacity capacity NN cord-266018-8bhnlsgy 283 21 of of IN cord-266018-8bhnlsgy 283 22 CD8alpha(+ CD8alpha(+ NNP cord-266018-8bhnlsgy 283 23 ) ) -RRB- cord-266018-8bhnlsgy 283 24 and and CC cord-266018-8bhnlsgy 283 25 CD8alpha(À cd8alpha(à LS cord-266018-8bhnlsgy 283 26 ) ) -RRB- cord-266018-8bhnlsgy 283 27 dendritic dendritic JJ cord-266018-8bhnlsgy 283 28 cells cell NNS cord-266018-8bhnlsgy 283 29 to to IN cord-266018-8bhnlsgy 283 30 prime prime JJ cord-266018-8bhnlsgy 283 31 Th1 Th1 NNP cord-266018-8bhnlsgy 283 32 / / SYM cord-266018-8bhnlsgy 283 33 Th2 th2 CD cord-266018-8bhnlsgy 283 34 cells cell NNS cord-266018-8bhnlsgy 283 35 in in IN cord-266018-8bhnlsgy 283 36 vivo vivo NNP cord-266018-8bhnlsgy 283 37 Kinetics Kinetics NNP cord-266018-8bhnlsgy 283 38 of of IN cord-266018-8bhnlsgy 283 39 virus virus NN cord-266018-8bhnlsgy 283 40 - - HYPH cord-266018-8bhnlsgy 283 41 specific specific JJ cord-266018-8bhnlsgy 283 42 CD8 CD8 NNP cord-266018-8bhnlsgy 283 43 + + CC cord-266018-8bhnlsgy 283 44 -T -T : cord-266018-8bhnlsgy 283 45 - - HYPH cord-266018-8bhnlsgy 283 46 cell cell NN cord-266018-8bhnlsgy 283 47 expansion expansion NN cord-266018-8bhnlsgy 283 48 and and CC cord-266018-8bhnlsgy 283 49 trafficking trafficking NN cord-266018-8bhnlsgy 283 50 following follow VBG cord-266018-8bhnlsgy 283 51 central central JJ cord-266018-8bhnlsgy 283 52 nervous nervous JJ cord-266018-8bhnlsgy 283 53 system system NN cord-266018-8bhnlsgy 283 54 infection infection NN cord-266018-8bhnlsgy 283 55 Chemokines Chemokines NNPS cord-266018-8bhnlsgy 283 56 and and CC cord-266018-8bhnlsgy 283 57 dendritic dendritic JJ cord-266018-8bhnlsgy 283 58 cells cell NNS cord-266018-8bhnlsgy 283 59 : : : cord-266018-8bhnlsgy 283 60 a a DT cord-266018-8bhnlsgy 283 61 crucial crucial JJ cord-266018-8bhnlsgy 283 62 alliance alliance NN cord-266018-8bhnlsgy 284 1 A a DT cord-266018-8bhnlsgy 284 2 mechanism mechanism NN cord-266018-8bhnlsgy 284 3 for for IN cord-266018-8bhnlsgy 284 4 the the DT cord-266018-8bhnlsgy 284 5 impaired impaired JJ cord-266018-8bhnlsgy 284 6 IFN IFN NNP cord-266018-8bhnlsgy 284 7 - - HYPH cord-266018-8bhnlsgy 284 8 gamma gamma NN cord-266018-8bhnlsgy 284 9 production production NN cord-266018-8bhnlsgy 284 10 in in IN cord-266018-8bhnlsgy 284 11 C C NNP cord-266018-8bhnlsgy 284 12 - - HYPH cord-266018-8bhnlsgy 284 13 C C NNP cord-266018-8bhnlsgy 284 14 chemokine chemokine NN cord-266018-8bhnlsgy 284 15 receptor receptor NN cord-266018-8bhnlsgy 284 16 2 2 CD cord-266018-8bhnlsgy 285 1 ( ( -LRB- cord-266018-8bhnlsgy 285 2 CCR2 CCR2 NNP cord-266018-8bhnlsgy 285 3 ) ) -RRB- cord-266018-8bhnlsgy 285 4 knockout knockout NN cord-266018-8bhnlsgy 285 5 mice mouse NNS cord-266018-8bhnlsgy 285 6 : : : cord-266018-8bhnlsgy 285 7 role role NN cord-266018-8bhnlsgy 285 8 of of IN cord-266018-8bhnlsgy 285 9 CCR2 CCR2 NNP cord-266018-8bhnlsgy 285 10 in in IN cord-266018-8bhnlsgy 285 11 linking link VBG cord-266018-8bhnlsgy 285 12 the the DT cord-266018-8bhnlsgy 285 13 innate innate JJ cord-266018-8bhnlsgy 285 14 and and CC cord-266018-8bhnlsgy 285 15 adaptive adaptive JJ cord-266018-8bhnlsgy 285 16 immune immune JJ cord-266018-8bhnlsgy 285 17 responses response NNS cord-266018-8bhnlsgy 285 18 Chemokine Chemokine NNP cord-266018-8bhnlsgy 285 19 receptor receptor NN cord-266018-8bhnlsgy 285 20 2 2 CD cord-266018-8bhnlsgy 285 21 serves serve VBZ cord-266018-8bhnlsgy 285 22 an an DT cord-266018-8bhnlsgy 285 23 early early JJ cord-266018-8bhnlsgy 285 24 and and CC cord-266018-8bhnlsgy 285 25 essential essential JJ cord-266018-8bhnlsgy 285 26 role role NN cord-266018-8bhnlsgy 285 27 in in IN cord-266018-8bhnlsgy 285 28 resistance resistance NN cord-266018-8bhnlsgy 285 29 to to IN cord-266018-8bhnlsgy 285 30 Mycobacterium Mycobacterium NNP cord-266018-8bhnlsgy 285 31 tuberculosis tuberculosis NN cord-266018-8bhnlsgy 285 32 Distinct distinct JJ cord-266018-8bhnlsgy 285 33 dendritic dendritic JJ cord-266018-8bhnlsgy 285 34 cell cell NN cord-266018-8bhnlsgy 285 35 subsets subset NNS cord-266018-8bhnlsgy 285 36 differentially differentially RB cord-266018-8bhnlsgy 285 37 regulate regulate VBP cord-266018-8bhnlsgy 285 38 the the DT cord-266018-8bhnlsgy 285 39 class class NN cord-266018-8bhnlsgy 285 40 of of IN cord-266018-8bhnlsgy 285 41 immune immune JJ cord-266018-8bhnlsgy 285 42 response response NN cord-266018-8bhnlsgy 285 43 in in IN cord-266018-8bhnlsgy 285 44 vivo vivo NN cord-266018-8bhnlsgy 286 1 In in IN cord-266018-8bhnlsgy 286 2 vivo vivo NN cord-266018-8bhnlsgy 286 3 microbial microbial JJ cord-266018-8bhnlsgy 286 4 stimulation stimulation NN cord-266018-8bhnlsgy 286 5 induces induce VBZ cord-266018-8bhnlsgy 286 6 rapid rapid JJ cord-266018-8bhnlsgy 286 7 CD40 CD40 NNP cord-266018-8bhnlsgy 286 8 ligand ligand NN cord-266018-8bhnlsgy 286 9 - - HYPH cord-266018-8bhnlsgy 286 10 independent independent JJ cord-266018-8bhnlsgy 286 11 production production NN cord-266018-8bhnlsgy 286 12 of of IN cord-266018-8bhnlsgy 286 13 interleukin interleukin NN cord-266018-8bhnlsgy 286 14 12 12 CD cord-266018-8bhnlsgy 286 15 by by IN cord-266018-8bhnlsgy 286 16 dendritic dendritic JJ cord-266018-8bhnlsgy 286 17 cells cell NNS cord-266018-8bhnlsgy 286 18 and and CC cord-266018-8bhnlsgy 286 19 their -PRON- PRP$ cord-266018-8bhnlsgy 286 20 redistribution redistribution NN cord-266018-8bhnlsgy 286 21 to to IN cord-266018-8bhnlsgy 286 22 T t NN cord-266018-8bhnlsgy 286 23 cell cell NN cord-266018-8bhnlsgy 286 24 areas area NNS cord-266018-8bhnlsgy 286 25 CC CC NNP cord-266018-8bhnlsgy 286 26 chemokine chemokine NN cord-266018-8bhnlsgy 286 27 receptor receptor NN cord-266018-8bhnlsgy 286 28 ( ( -LRB- cord-266018-8bhnlsgy 286 29 CCR)2 ccr)2 NN cord-266018-8bhnlsgy 286 30 is be VBZ cord-266018-8bhnlsgy 286 31 required require VBN cord-266018-8bhnlsgy 286 32 for for IN cord-266018-8bhnlsgy 286 33 Langerhans Langerhans NNP cord-266018-8bhnlsgy 286 34 cell cell NN cord-266018-8bhnlsgy 286 35 migration migration NN cord-266018-8bhnlsgy 286 36 and and CC cord-266018-8bhnlsgy 286 37 localization localization NN cord-266018-8bhnlsgy 286 38 of of IN cord-266018-8bhnlsgy 286 39 T T NNP cord-266018-8bhnlsgy 286 40 helper helper NN cord-266018-8bhnlsgy 286 41 cell cell NN cord-266018-8bhnlsgy 286 42 type type NN cord-266018-8bhnlsgy 286 43 1 1 CD cord-266018-8bhnlsgy 286 44 ( ( -LRB- cord-266018-8bhnlsgy 286 45 Th1)-inducing th1)-induce VBG cord-266018-8bhnlsgy 286 46 dendritic dendritic JJ cord-266018-8bhnlsgy 286 47 cells cell NNS cord-266018-8bhnlsgy 286 48 . . . cord-266018-8bhnlsgy 287 1 Absence absence NN cord-266018-8bhnlsgy 287 2 of of IN cord-266018-8bhnlsgy 287 3 CCR2 CCR2 NNP cord-266018-8bhnlsgy 287 4 shifts shift NNS cord-266018-8bhnlsgy 287 5 the the DT cord-266018-8bhnlsgy 287 6 Leishmania Leishmania NNP cord-266018-8bhnlsgy 287 7 major major JJ cord-266018-8bhnlsgy 287 8 - - HYPH cord-266018-8bhnlsgy 287 9 resistant resistant JJ cord-266018-8bhnlsgy 287 10 phenotype phenotype NN cord-266018-8bhnlsgy 287 11 to to IN cord-266018-8bhnlsgy 287 12 a a DT cord-266018-8bhnlsgy 287 13 susceptible susceptible JJ cord-266018-8bhnlsgy 287 14 state state NN cord-266018-8bhnlsgy 287 15 dominated dominate VBN cord-266018-8bhnlsgy 287 16 by by IN cord-266018-8bhnlsgy 287 17 Th2 Th2 NNP cord-266018-8bhnlsgy 287 18 cytokines cytokine NNS cord-266018-8bhnlsgy 287 19 , , , cord-266018-8bhnlsgy 287 20 B b NN cord-266018-8bhnlsgy 287 21 cell cell NN cord-266018-8bhnlsgy 287 22 outgrowth outgrowth NN cord-266018-8bhnlsgy 287 23 , , , cord-266018-8bhnlsgy 287 24 and and CC cord-266018-8bhnlsgy 287 25 sustained sustain VBD cord-266018-8bhnlsgy 287 26 neutrophilic neutrophilic JJ cord-266018-8bhnlsgy 287 27 inflammation inflammation NN cord-266018-8bhnlsgy 287 28 TNF TNF NNP cord-266018-8bhnlsgy 287 29 / / SYM cord-266018-8bhnlsgy 287 30 iNOS inos NN cord-266018-8bhnlsgy 287 31 - - HYPH cord-266018-8bhnlsgy 287 32 producing produce VBG cord-266018-8bhnlsgy 287 33 dendritic dendritic JJ cord-266018-8bhnlsgy 287 34 cells cell NNS cord-266018-8bhnlsgy 287 35 mediate mediate VBP cord-266018-8bhnlsgy 287 36 innate innate JJ cord-266018-8bhnlsgy 287 37 immune immune JJ cord-266018-8bhnlsgy 287 38 defense defense NN cord-266018-8bhnlsgy 287 39 against against IN cord-266018-8bhnlsgy 287 40 bacterial bacterial JJ cord-266018-8bhnlsgy 287 41 infection infection NN cord-266018-8bhnlsgy 288 1 The the DT cord-266018-8bhnlsgy 288 2 CC cc NN cord-266018-8bhnlsgy 288 3 chemokine chemokine NN cord-266018-8bhnlsgy 288 4 ligand ligand NN cord-266018-8bhnlsgy 288 5 3 3 CD cord-266018-8bhnlsgy 289 1 ( ( -LRB- cord-266018-8bhnlsgy 289 2 CCL3 CCL3 NNP cord-266018-8bhnlsgy 289 3 ) ) -RRB- cord-266018-8bhnlsgy 289 4 regulates regulate VBZ cord-266018-8bhnlsgy 289 5 CD8 CD8 NNP cord-266018-8bhnlsgy 289 6 + + CC cord-266018-8bhnlsgy 289 7 T T NNP cord-266018-8bhnlsgy 289 8 cell cell NN cord-266018-8bhnlsgy 289 9 effector effector NN cord-266018-8bhnlsgy 289 10 function function NN cord-266018-8bhnlsgy 289 11 and and CC cord-266018-8bhnlsgy 289 12 migration migration NN cord-266018-8bhnlsgy 289 13 following follow VBG cord-266018-8bhnlsgy 289 14 viral viral JJ cord-266018-8bhnlsgy 289 15 infection infection NN cord-266018-8bhnlsgy 289 16 Identification identification NN cord-266018-8bhnlsgy 289 17 of of IN cord-266018-8bhnlsgy 289 18 a a DT cord-266018-8bhnlsgy 289 19 CD4 CD4 NNP cord-266018-8bhnlsgy 289 20 + + CC cord-266018-8bhnlsgy 289 21 T t NN cord-266018-8bhnlsgy 289 22 cell cell NN cord-266018-8bhnlsgy 289 23 epitope epitope NN cord-266018-8bhnlsgy 289 24 within within IN cord-266018-8bhnlsgy 289 25 the the DT cord-266018-8bhnlsgy 289 26 M M NNP cord-266018-8bhnlsgy 289 27 protein protein NN cord-266018-8bhnlsgy 289 28 of of IN cord-266018-8bhnlsgy 289 29 a a DT cord-266018-8bhnlsgy 289 30 neurotropic neurotropic NNP cord-266018-8bhnlsgy 289 31 coronavirus coronavirus NN cord-266018-8bhnlsgy 290 1 This this DT cord-266018-8bhnlsgy 290 2 work work NN cord-266018-8bhnlsgy 290 3 was be VBD cord-266018-8bhnlsgy 290 4 supported support VBN cord-266018-8bhnlsgy 290 5 by by IN cord-266018-8bhnlsgy 290 6 National National NNP cord-266018-8bhnlsgy 290 7 Institutes Institutes NNPS cord-266018-8bhnlsgy 290 8 of of IN cord-266018-8bhnlsgy 290 9 Health Health NNP cord-266018-8bhnlsgy 290 10 Grant Grant NNP cord-266018-8bhnlsgy 290 11 41249 41249 CD cord-266018-8bhnlsgy 290 12 and and CC cord-266018-8bhnlsgy 290 13 National National NNP cord-266018-8bhnlsgy 290 14 Multiple Multiple NNP cord-266018-8bhnlsgy 290 15 Sclerosis Sclerosis NNP cord-266018-8bhnlsgy 290 16 Society Society NNP cord-266018-8bhnlsgy 290 17 Grant Grant NNP cord-266018-8bhnlsgy 290 18 3278-A-3 3278-a-3 CD cord-266018-8bhnlsgy 290 19 to to IN cord-266018-8bhnlsgy 290 20 T.E.L. t.e.l. NN