id	sid	tid	token	lemma	pos
cord-268139-tgpsu4qz	1	1	key	key	NN
cord-268139-tgpsu4qz	1	2	:	:	:
cord-268139-tgpsu4qz	2	1	cord-268139-tgpsu4qz	cord-268139-tgpsu4qz	LS
cord-268139-tgpsu4qz	2	2	authors	author	NNS
cord-268139-tgpsu4qz	2	3	:	:	:
cord-268139-tgpsu4qz	2	4	Brockway	Brockway	NNP
cord-268139-tgpsu4qz	2	5	,	,	,
cord-268139-tgpsu4qz	2	6	Sarah	Sarah	NNP
cord-268139-tgpsu4qz	2	7	M.	M.	NNP
cord-268139-tgpsu4qz	2	8	;	;	:
cord-268139-tgpsu4qz	2	9	Denison	Denison	NNP
cord-268139-tgpsu4qz	2	10	,	,	,
cord-268139-tgpsu4qz	2	11	Mark	Mark	NNP
cord-268139-tgpsu4qz	2	12	R.	R.	NNP
cord-268139-tgpsu4qz	2	13	title	title	NN
cord-268139-tgpsu4qz	2	14	:	:	:
cord-268139-tgpsu4qz	2	15	Mutagenesis	Mutagenesis	NNP
cord-268139-tgpsu4qz	2	16	of	of	IN
cord-268139-tgpsu4qz	2	17	the	the	DT
cord-268139-tgpsu4qz	2	18	murine	murine	JJ
cord-268139-tgpsu4qz	2	19	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	2	20	virus	virus	NN
cord-268139-tgpsu4qz	3	1	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	3	2	region	region	NN
cord-268139-tgpsu4qz	3	3	identifies	identify	VBZ
cord-268139-tgpsu4qz	3	4	residues	residue	NNS
cord-268139-tgpsu4qz	3	5	important	important	JJ
cord-268139-tgpsu4qz	3	6	for	for	IN
cord-268139-tgpsu4qz	3	7	protein	protein	NN
cord-268139-tgpsu4qz	3	8	processing	processing	NN
cord-268139-tgpsu4qz	3	9	,	,	,
cord-268139-tgpsu4qz	3	10	viral	viral	JJ
cord-268139-tgpsu4qz	3	11	RNA	RNA	NNP
cord-268139-tgpsu4qz	3	12	synthesis	synthesis	NN
cord-268139-tgpsu4qz	3	13	,	,	,
cord-268139-tgpsu4qz	3	14	and	and	CC
cord-268139-tgpsu4qz	3	15	viral	viral	JJ
cord-268139-tgpsu4qz	3	16	replication	replication	NN
cord-268139-tgpsu4qz	3	17	date	date	NN
cord-268139-tgpsu4qz	3	18	:	:	:
cord-268139-tgpsu4qz	3	19	2005	2005	CD
cord-268139-tgpsu4qz	3	20	-	-	HYPH
cord-268139-tgpsu4qz	3	21	09	09	CD
cord-268139-tgpsu4qz	3	22	-	-	SYM
cord-268139-tgpsu4qz	3	23	30	30	CD
cord-268139-tgpsu4qz	3	24	journal	journal	NN
cord-268139-tgpsu4qz	3	25	:	:	:
cord-268139-tgpsu4qz	4	1	Virology	virology	NN
cord-268139-tgpsu4qz	4	2	DOI	DOI	NNP
cord-268139-tgpsu4qz	4	3	:	:	:
cord-268139-tgpsu4qz	4	4	10.1016/	10.1016/	CD
cord-268139-tgpsu4qz	5	1	j.virol.2005.06.035	j.virol.2005.06.035	NNP
cord-268139-tgpsu4qz	5	2	sha	sha	XX
cord-268139-tgpsu4qz	5	3	:	:	:
cord-268139-tgpsu4qz	6	1	b067a208058f1edd575cb8c0c6726674bd66af50	b067a208058f1edd575cb8c0c6726674bd66af50	NNP
cord-268139-tgpsu4qz	6	2	doc_id	doc_id	CD
cord-268139-tgpsu4qz	6	3	:	:	:
cord-268139-tgpsu4qz	6	4	268139	268139	CD
cord-268139-tgpsu4qz	6	5	cord_uid	cord_uid	NNS
cord-268139-tgpsu4qz	6	6	:	:	:
cord-268139-tgpsu4qz	7	1	tgpsu4qz	tgpsu4qz	.
cord-268139-tgpsu4qz	7	2	Despite	despite	IN
cord-268139-tgpsu4qz	7	3	ongoing	ongoing	JJ
cord-268139-tgpsu4qz	7	4	research	research	NN
cord-268139-tgpsu4qz	7	5	investigating	investigate	VBG
cord-268139-tgpsu4qz	7	6	mechanisms	mechanism	NNS
cord-268139-tgpsu4qz	7	7	of	of	IN
cord-268139-tgpsu4qz	7	8	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	7	9	replication	replication	NN
cord-268139-tgpsu4qz	7	10	,	,	,
cord-268139-tgpsu4qz	7	11	functions	function	NNS
cord-268139-tgpsu4qz	7	12	of	of	IN
cord-268139-tgpsu4qz	7	13	many	many	JJ
cord-268139-tgpsu4qz	7	14	viral	viral	JJ
cord-268139-tgpsu4qz	7	15	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	7	16	proteins	protein	NNS
cord-268139-tgpsu4qz	7	17	(	(	-LRB-
cord-268139-tgpsu4qz	7	18	nsps	nsps	NNP
cord-268139-tgpsu4qz	7	19	)	)	-RRB-
cord-268139-tgpsu4qz	7	20	remain	remain	VBP
cord-268139-tgpsu4qz	7	21	unknown	unknown	JJ
cord-268139-tgpsu4qz	7	22	.	.	.
cord-268139-tgpsu4qz	8	1	In	in	IN
cord-268139-tgpsu4qz	8	2	the	the	DT
cord-268139-tgpsu4qz	8	3	current	current	JJ
cord-268139-tgpsu4qz	8	4	study	study	NN
cord-268139-tgpsu4qz	8	5	,	,	,
cord-268139-tgpsu4qz	8	6	a	a	DT
cord-268139-tgpsu4qz	8	7	reverse	reverse	JJ
cord-268139-tgpsu4qz	8	8	genetic	genetic	JJ
cord-268139-tgpsu4qz	8	9	approach	approach	NN
cord-268139-tgpsu4qz	8	10	was	be	VBD
cord-268139-tgpsu4qz	8	11	used	use	VBN
cord-268139-tgpsu4qz	8	12	to	to	TO
cord-268139-tgpsu4qz	8	13	define	define	VB
cord-268139-tgpsu4qz	8	14	the	the	DT
cord-268139-tgpsu4qz	8	15	role	role	NN
cord-268139-tgpsu4qz	8	16	of	of	IN
cord-268139-tgpsu4qz	8	17	the	the	DT
cord-268139-tgpsu4qz	8	18	28-kDa	28-kda	CD
cord-268139-tgpsu4qz	8	19	amino	amino	NN
cord-268139-tgpsu4qz	8	20	-	-	HYPH
cord-268139-tgpsu4qz	8	21	terminal	terminal	NN
cord-268139-tgpsu4qz	8	22	product	product	NN
cord-268139-tgpsu4qz	8	23	(	(	-LRB-
cord-268139-tgpsu4qz	8	24	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	8	25	)	)	-RRB-
cord-268139-tgpsu4qz	8	26	of	of	IN
cord-268139-tgpsu4qz	8	27	the	the	DT
cord-268139-tgpsu4qz	8	28	gene	gene	NN
cord-268139-tgpsu4qz	8	29	1	1	CD
cord-268139-tgpsu4qz	8	30	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	8	31	during	during	IN
cord-268139-tgpsu4qz	8	32	replication	replication	NN
cord-268139-tgpsu4qz	8	33	of	of	IN
cord-268139-tgpsu4qz	8	34	the	the	DT
cord-268139-tgpsu4qz	8	35	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	8	36	murine	murine	JJ
cord-268139-tgpsu4qz	8	37	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	8	38	virus	virus	NN
cord-268139-tgpsu4qz	8	39	(	(	-LRB-
cord-268139-tgpsu4qz	8	40	MHV	MHV	NNP
cord-268139-tgpsu4qz	8	41	)	)	-RRB-
cord-268139-tgpsu4qz	8	42	in	in	IN
cord-268139-tgpsu4qz	8	43	cell	cell	NN
cord-268139-tgpsu4qz	8	44	culture	culture	NN
cord-268139-tgpsu4qz	8	45	.	.	.
cord-268139-tgpsu4qz	9	1	To	to	TO
cord-268139-tgpsu4qz	9	2	determine	determine	VB
cord-268139-tgpsu4qz	9	3	whether	whether	IN
cord-268139-tgpsu4qz	9	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	9	5	is	be	VBZ
cord-268139-tgpsu4qz	9	6	required	require	VBN
cord-268139-tgpsu4qz	9	7	for	for	IN
cord-268139-tgpsu4qz	9	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	9	9	replication	replication	NN
cord-268139-tgpsu4qz	9	10	and	and	CC
cord-268139-tgpsu4qz	9	11	to	to	TO
cord-268139-tgpsu4qz	9	12	identify	identify	VB
cord-268139-tgpsu4qz	9	13	residues	residue	NNS
cord-268139-tgpsu4qz	9	14	critical	critical	JJ
cord-268139-tgpsu4qz	9	15	for	for	IN
cord-268139-tgpsu4qz	9	16	protein	protein	NN
cord-268139-tgpsu4qz	9	17	function	function	NN
cord-268139-tgpsu4qz	9	18	,	,	,
cord-268139-tgpsu4qz	9	19	mutant	mutant	JJ
cord-268139-tgpsu4qz	9	20	viruses	virus	NNS
cord-268139-tgpsu4qz	9	21	that	that	WDT
cord-268139-tgpsu4qz	9	22	contained	contain	VBD
cord-268139-tgpsu4qz	9	23	deletions	deletion	NNS
cord-268139-tgpsu4qz	9	24	or	or	CC
cord-268139-tgpsu4qz	9	25	point	point	NN
cord-268139-tgpsu4qz	9	26	mutations	mutation	NNS
cord-268139-tgpsu4qz	9	27	within	within	IN
cord-268139-tgpsu4qz	9	28	the	the	DT
cord-268139-tgpsu4qz	9	29	nsp1-coding	nsp1-coding	NNP
cord-268139-tgpsu4qz	9	30	region	region	NN
cord-268139-tgpsu4qz	9	31	were	be	VBD
cord-268139-tgpsu4qz	9	32	generated	generate	VBN
cord-268139-tgpsu4qz	9	33	and	and	CC
cord-268139-tgpsu4qz	9	34	assayed	assay	VBN
cord-268139-tgpsu4qz	9	35	for	for	IN
cord-268139-tgpsu4qz	9	36	defects	defect	NNS
cord-268139-tgpsu4qz	9	37	in	in	IN
cord-268139-tgpsu4qz	9	38	viral	viral	JJ
cord-268139-tgpsu4qz	9	39	replication	replication	NN
cord-268139-tgpsu4qz	9	40	,	,	,
cord-268139-tgpsu4qz	9	41	viral	viral	JJ
cord-268139-tgpsu4qz	9	42	protein	protein	NN
cord-268139-tgpsu4qz	9	43	expression	expression	NN
cord-268139-tgpsu4qz	9	44	,	,	,
cord-268139-tgpsu4qz	9	45	protein	protein	NN
cord-268139-tgpsu4qz	9	46	localization	localization	NN
cord-268139-tgpsu4qz	9	47	,	,	,
cord-268139-tgpsu4qz	9	48	and	and	CC
cord-268139-tgpsu4qz	9	49	RNA	RNA	NNP
cord-268139-tgpsu4qz	9	50	synthesis	synthesis	NN
cord-268139-tgpsu4qz	9	51	.	.	.
cord-268139-tgpsu4qz	10	1	The	the	DT
cord-268139-tgpsu4qz	10	2	results	result	NNS
cord-268139-tgpsu4qz	10	3	demonstrated	demonstrate	VBD
cord-268139-tgpsu4qz	10	4	that	that	IN
cord-268139-tgpsu4qz	10	5	the	the	DT
cord-268139-tgpsu4qz	10	6	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	10	7	-	-	HYPH
cord-268139-tgpsu4qz	10	8	terminal	terminal	NN
cord-268139-tgpsu4qz	10	9	half	half	NN
cord-268139-tgpsu4qz	10	10	of	of	IN
cord-268139-tgpsu4qz	10	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	10	12	(	(	-LRB-
cord-268139-tgpsu4qz	10	13	residues	residue	NNS
cord-268139-tgpsu4qz	10	14	K(124	k(124	VBP
cord-268139-tgpsu4qz	10	15	)	)	-RRB-
cord-268139-tgpsu4qz	10	16	through	through	IN
cord-268139-tgpsu4qz	10	17	L(241	l(241	NN
cord-268139-tgpsu4qz	10	18	)	)	-RRB-
cord-268139-tgpsu4qz	10	19	)	)	-RRB-
cord-268139-tgpsu4qz	10	20	was	be	VBD
cord-268139-tgpsu4qz	10	21	dispensable	dispensable	JJ
cord-268139-tgpsu4qz	10	22	for	for	IN
cord-268139-tgpsu4qz	10	23	virus	virus	NN
cord-268139-tgpsu4qz	10	24	replication	replication	NN
cord-268139-tgpsu4qz	10	25	in	in	IN
cord-268139-tgpsu4qz	10	26	culture	culture	NN
cord-268139-tgpsu4qz	10	27	but	but	CC
cord-268139-tgpsu4qz	10	28	was	be	VBD
cord-268139-tgpsu4qz	10	29	required	require	VBN
cord-268139-tgpsu4qz	10	30	for	for	IN
cord-268139-tgpsu4qz	10	31	efficient	efficient	JJ
cord-268139-tgpsu4qz	10	32	proteolytic	proteolytic	JJ
cord-268139-tgpsu4qz	10	33	cleavage	cleavage	NN
cord-268139-tgpsu4qz	10	34	of	of	IN
cord-268139-tgpsu4qz	10	35	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	10	36	from	from	IN
cord-268139-tgpsu4qz	10	37	the	the	DT
cord-268139-tgpsu4qz	10	38	gene	gene	NN
cord-268139-tgpsu4qz	10	39	1	1	CD
cord-268139-tgpsu4qz	10	40	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	10	41	and	and	CC
cord-268139-tgpsu4qz	10	42	for	for	IN
cord-268139-tgpsu4qz	10	43	optimal	optimal	JJ
cord-268139-tgpsu4qz	10	44	viral	viral	JJ
cord-268139-tgpsu4qz	10	45	replication	replication	NN
cord-268139-tgpsu4qz	10	46	.	.	.
cord-268139-tgpsu4qz	11	1	Furthermore	furthermore	RB
cord-268139-tgpsu4qz	11	2	,	,	,
cord-268139-tgpsu4qz	11	3	whereas	whereas	IN
cord-268139-tgpsu4qz	11	4	deletion	deletion	NN
cord-268139-tgpsu4qz	11	5	of	of	IN
cord-268139-tgpsu4qz	11	6	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	11	7	residues	residue	NNS
cord-268139-tgpsu4qz	11	8	amino	amino	NN
cord-268139-tgpsu4qz	11	9	-	-	HYPH
cord-268139-tgpsu4qz	11	10	terminal	terminal	NN
cord-268139-tgpsu4qz	11	11	to	to	IN
cord-268139-tgpsu4qz	11	12	K(124	K(124	NNP
cord-268139-tgpsu4qz	11	13	)	)	-RRB-
cord-268139-tgpsu4qz	11	14	failed	fail	VBD
cord-268139-tgpsu4qz	11	15	to	to	TO
cord-268139-tgpsu4qz	11	16	produce	produce	VB
cord-268139-tgpsu4qz	11	17	infectious	infectious	JJ
cord-268139-tgpsu4qz	11	18	virus	virus	NN
cord-268139-tgpsu4qz	11	19	,	,	,
cord-268139-tgpsu4qz	11	20	point	point	NN
cord-268139-tgpsu4qz	11	21	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	11	22	of	of	IN
cord-268139-tgpsu4qz	11	23	the	the	DT
cord-268139-tgpsu4qz	11	24	nsp1	nsp1	CD
cord-268139-tgpsu4qz	11	25	amino	amino	NN
cord-268139-tgpsu4qz	11	26	-	-	HYPH
cord-268139-tgpsu4qz	11	27	terminus	terminus	NN
cord-268139-tgpsu4qz	11	28	allowed	allow	VBD
cord-268139-tgpsu4qz	11	29	recovery	recovery	NN
cord-268139-tgpsu4qz	11	30	of	of	IN
cord-268139-tgpsu4qz	11	31	several	several	JJ
cord-268139-tgpsu4qz	11	32	mutants	mutant	NNS
cord-268139-tgpsu4qz	11	33	with	with	IN
cord-268139-tgpsu4qz	11	34	altered	altered	JJ
cord-268139-tgpsu4qz	11	35	replication	replication	NN
cord-268139-tgpsu4qz	11	36	and	and	CC
cord-268139-tgpsu4qz	11	37	RNA	RNA	NNP
cord-268139-tgpsu4qz	11	38	synthesis	synthesis	NN
cord-268139-tgpsu4qz	11	39	.	.	.
cord-268139-tgpsu4qz	12	1	This	this	DT
cord-268139-tgpsu4qz	12	2	study	study	NN
cord-268139-tgpsu4qz	12	3	identifies	identify	VBZ
cord-268139-tgpsu4qz	12	4	nsp1	nsp1	NN
cord-268139-tgpsu4qz	12	5	residues	residue	NNS
cord-268139-tgpsu4qz	12	6	important	important	JJ
cord-268139-tgpsu4qz	12	7	for	for	IN
cord-268139-tgpsu4qz	12	8	protein	protein	NN
cord-268139-tgpsu4qz	12	9	processing	processing	NN
cord-268139-tgpsu4qz	12	10	,	,	,
cord-268139-tgpsu4qz	12	11	viral	viral	JJ
cord-268139-tgpsu4qz	12	12	RNA	RNA	NNP
cord-268139-tgpsu4qz	12	13	synthesis	synthesis	NN
cord-268139-tgpsu4qz	12	14	,	,	,
cord-268139-tgpsu4qz	12	15	and	and	CC
cord-268139-tgpsu4qz	12	16	viral	viral	JJ
cord-268139-tgpsu4qz	12	17	replication	replication	NN
cord-268139-tgpsu4qz	12	18	.	.	.
cord-268139-tgpsu4qz	13	1	Mutagenesis	Mutagenesis	NNP
cord-268139-tgpsu4qz	13	2	of	of	IN
cord-268139-tgpsu4qz	13	3	the	the	DT
cord-268139-tgpsu4qz	13	4	murine	murine	JJ
cord-268139-tgpsu4qz	13	5	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	13	6	virus	virus	NN
cord-268139-tgpsu4qz	14	1	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	14	2	region	region	NN
cord-268139-tgpsu4qz	14	3	identifies	identify	VBZ
cord-268139-tgpsu4qz	14	4	residues	residue	NNS
cord-268139-tgpsu4qz	14	5	important	important	JJ
cord-268139-tgpsu4qz	14	6	for	for	IN
cord-268139-tgpsu4qz	14	7	protein	protein	NN
cord-268139-tgpsu4qz	14	8	processing	processing	NN
cord-268139-tgpsu4qz	14	9	,	,	,
cord-268139-tgpsu4qz	14	10	viral	viral	JJ
cord-268139-tgpsu4qz	14	11	RNA	RNA	NNP
cord-268139-tgpsu4qz	14	12	synthesis	synthesis	NN
cord-268139-tgpsu4qz	14	13	,	,	,
cord-268139-tgpsu4qz	14	14	and	and	CC
cord-268139-tgpsu4qz	14	15	viral	viral	JJ
cord-268139-tgpsu4qz	14	16	replication	replication	NN
cord-268139-tgpsu4qz	14	17	Coronaviruses	coronaviruse	NNS
cord-268139-tgpsu4qz	14	18	belong	belong	VBP
cord-268139-tgpsu4qz	14	19	to	to	IN
cord-268139-tgpsu4qz	14	20	a	a	DT
cord-268139-tgpsu4qz	14	21	family	family	NN
cord-268139-tgpsu4qz	14	22	of	of	IN
cord-268139-tgpsu4qz	14	23	enveloped	envelop	VBN
cord-268139-tgpsu4qz	14	24	positivestrand	positivestrand	NNP
cord-268139-tgpsu4qz	14	25	RNA	RNA	NNP
cord-268139-tgpsu4qz	14	26	viruses	virus	NNS
cord-268139-tgpsu4qz	14	27	that	that	WDT
cord-268139-tgpsu4qz	14	28	are	be	VBP
cord-268139-tgpsu4qz	14	29	responsible	responsible	JJ
cord-268139-tgpsu4qz	14	30	for	for	IN
cord-268139-tgpsu4qz	14	31	devastating	devastating	JJ
cord-268139-tgpsu4qz	14	32	illnesses	illness	NNS
cord-268139-tgpsu4qz	14	33	in	in	IN
cord-268139-tgpsu4qz	14	34	livestock	livestock	NN
cord-268139-tgpsu4qz	14	35	and	and	CC
cord-268139-tgpsu4qz	14	36	domestic	domestic	JJ
cord-268139-tgpsu4qz	14	37	animals	animal	NNS
cord-268139-tgpsu4qz	14	38	.	.	.
cord-268139-tgpsu4qz	15	1	The	the	DT
cord-268139-tgpsu4qz	15	2	identification	identification	NN
cord-268139-tgpsu4qz	15	3	of	of	IN
cord-268139-tgpsu4qz	15	4	a	a	DT
cord-268139-tgpsu4qz	15	5	novel	novel	JJ
cord-268139-tgpsu4qz	15	6	human	human	JJ
cord-268139-tgpsu4qz	15	7	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	15	8	as	as	IN
cord-268139-tgpsu4qz	15	9	the	the	DT
cord-268139-tgpsu4qz	15	10	etiological	etiological	JJ
cord-268139-tgpsu4qz	15	11	agent	agent	NN
cord-268139-tgpsu4qz	15	12	of	of	IN
cord-268139-tgpsu4qz	15	13	Severe	severe	JJ
cord-268139-tgpsu4qz	15	14	Acute	acute	JJ
cord-268139-tgpsu4qz	15	15	Respiratory	Respiratory	NNP
cord-268139-tgpsu4qz	15	16	Syndrome	Syndrome	NNP
cord-268139-tgpsu4qz	15	17	(	(	-LRB-
cord-268139-tgpsu4qz	15	18	SARS	SARS	NNP
cord-268139-tgpsu4qz	15	19	)	)	-RRB-
cord-268139-tgpsu4qz	15	20	in	in	IN
cord-268139-tgpsu4qz	15	21	2003	2003	CD
cord-268139-tgpsu4qz	15	22	highlighted	highlight	VBD
cord-268139-tgpsu4qz	15	23	the	the	DT
cord-268139-tgpsu4qz	15	24	potential	potential	NN
cord-268139-tgpsu4qz	15	25	of	of	IN
cord-268139-tgpsu4qz	15	26	this	this	DT
cord-268139-tgpsu4qz	15	27	virus	virus	NN
cord-268139-tgpsu4qz	15	28	family	family	NN
cord-268139-tgpsu4qz	15	29	to	to	TO
cord-268139-tgpsu4qz	15	30	also	also	RB
cord-268139-tgpsu4qz	15	31	cause	cause	VB
cord-268139-tgpsu4qz	15	32	severe	severe	JJ
cord-268139-tgpsu4qz	15	33	human	human	JJ
cord-268139-tgpsu4qz	15	34	disease	disease	NN
cord-268139-tgpsu4qz	15	35	(	(	-LRB-
cord-268139-tgpsu4qz	15	36	Kuiken	Kuiken	NNP
cord-268139-tgpsu4qz	15	37	et	et	FW
cord-268139-tgpsu4qz	15	38	al	al	NNP
cord-268139-tgpsu4qz	15	39	.	.	NNP
cord-268139-tgpsu4qz	15	40	,	,	,
cord-268139-tgpsu4qz	15	41	2003	2003	CD
cord-268139-tgpsu4qz	15	42	)	)	-RRB-
cord-268139-tgpsu4qz	15	43	.	.	.
cord-268139-tgpsu4qz	16	1	Even	even	RB
cord-268139-tgpsu4qz	16	2	with	with	IN
cord-268139-tgpsu4qz	16	3	continuing	continue	VBG
cord-268139-tgpsu4qz	16	4	research	research	NN
cord-268139-tgpsu4qz	16	5	addressing	address	VBG
cord-268139-tgpsu4qz	16	6	how	how	WRB
cord-268139-tgpsu4qz	16	7	coronaviruses	coronaviruse	NNS
cord-268139-tgpsu4qz	16	8	replicate	replicate	VBP
cord-268139-tgpsu4qz	16	9	and	and	CC
cord-268139-tgpsu4qz	16	10	cause	cause	VB
cord-268139-tgpsu4qz	16	11	disease	disease	NN
cord-268139-tgpsu4qz	16	12	,	,	,
cord-268139-tgpsu4qz	16	13	the	the	DT
cord-268139-tgpsu4qz	16	14	functions	function	NNS
cord-268139-tgpsu4qz	16	15	of	of	IN
cord-268139-tgpsu4qz	16	16	many	many	JJ
cord-268139-tgpsu4qz	16	17	viral	viral	JJ
cord-268139-tgpsu4qz	16	18	proteins	protein	NNS
cord-268139-tgpsu4qz	16	19	remain	remain	VBP
cord-268139-tgpsu4qz	16	20	to	to	TO
cord-268139-tgpsu4qz	16	21	be	be	VB
cord-268139-tgpsu4qz	16	22	elucidated	elucidate	VBN
cord-268139-tgpsu4qz	16	23	.	.	.
cord-268139-tgpsu4qz	17	1	For	for	IN
cord-268139-tgpsu4qz	17	2	example	example	NN
cord-268139-tgpsu4qz	17	3	,	,	,
cord-268139-tgpsu4qz	17	4	14	14	CD
cord-268139-tgpsu4qz	17	5	-16	-16	CD
cord-268139-tgpsu4qz	17	6	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	17	7	proteins	protein	NNS
cord-268139-tgpsu4qz	17	8	(	(	-LRB-
cord-268139-tgpsu4qz	17	9	nsps	nsps	NNP
cord-268139-tgpsu4qz	17	10	)	)	-RRB-
cord-268139-tgpsu4qz	17	11	are	be	VBP
cord-268139-tgpsu4qz	17	12	expressed	express	VBN
cord-268139-tgpsu4qz	17	13	from	from	IN
cord-268139-tgpsu4qz	17	14	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	17	15	gene	gene	NN
cord-268139-tgpsu4qz	17	16	1	1	CD
cord-268139-tgpsu4qz	17	17	polyproteins	polyprotein	NNS
cord-268139-tgpsu4qz	17	18	,	,	,
cord-268139-tgpsu4qz	17	19	but	but	CC
cord-268139-tgpsu4qz	17	20	at	at	RB
cord-268139-tgpsu4qz	17	21	least	least	RBS
cord-268139-tgpsu4qz	17	22	seven	seven	CD
cord-268139-tgpsu4qz	17	23	of	of	IN
cord-268139-tgpsu4qz	17	24	these	these	DT
cord-268139-tgpsu4qz	17	25	nsps	nsp	NNS
cord-268139-tgpsu4qz	17	26	have	have	VBP
cord-268139-tgpsu4qz	17	27	no	no	DT
cord-268139-tgpsu4qz	17	28	known	know	VBN
cord-268139-tgpsu4qz	17	29	roles	role	NNS
cord-268139-tgpsu4qz	17	30	in	in	IN
cord-268139-tgpsu4qz	17	31	viral	viral	JJ
cord-268139-tgpsu4qz	17	32	replication	replication	NN
cord-268139-tgpsu4qz	17	33	.	.	.
cord-268139-tgpsu4qz	18	1	In	in	IN
cord-268139-tgpsu4qz	18	2	the	the	DT
cord-268139-tgpsu4qz	18	3	past	past	NN
cord-268139-tgpsu4qz	18	4	,	,	,
cord-268139-tgpsu4qz	18	5	gene	gene	NN
cord-268139-tgpsu4qz	18	6	1	1	CD
cord-268139-tgpsu4qz	18	7	has	have	VBZ
cord-268139-tgpsu4qz	18	8	been	be	VBN
cord-268139-tgpsu4qz	18	9	referred	refer	VBN
cord-268139-tgpsu4qz	18	10	to	to	IN
cord-268139-tgpsu4qz	18	11	as	as	IN
cord-268139-tgpsu4qz	18	12	the	the	DT
cord-268139-tgpsu4qz	18	13	'	'	``
cord-268139-tgpsu4qz	18	14	'	'	``
cord-268139-tgpsu4qz	18	15	replicase	replicase	NNP
cord-268139-tgpsu4qz	18	16	gene	gene	NNP
cord-268139-tgpsu4qz	18	17	'	'	''
cord-268139-tgpsu4qz	18	18	'	'	''
cord-268139-tgpsu4qz	18	19	and	and	CC
cord-268139-tgpsu4qz	18	20	gene	gene	NN
cord-268139-tgpsu4qz	18	21	1	1	CD
cord-268139-tgpsu4qz	18	22	nsps	nsp	NNS
cord-268139-tgpsu4qz	18	23	as	as	IN
cord-268139-tgpsu4qz	18	24	'	'	''
cord-268139-tgpsu4qz	18	25	'	'	``
cord-268139-tgpsu4qz	18	26	replicase	replicase	NNP
cord-268139-tgpsu4qz	18	27	proteins	protein	NNS
cord-268139-tgpsu4qz	18	28	'	'	POS
cord-268139-tgpsu4qz	18	29	'	'	''
cord-268139-tgpsu4qz	18	30	named	name	VBN
cord-268139-tgpsu4qz	18	31	by	by	IN
cord-268139-tgpsu4qz	18	32	their	-PRON-	PRP$
cord-268139-tgpsu4qz	18	33	molecular	molecular	JJ
cord-268139-tgpsu4qz	18	34	weight	weight	NN
cord-268139-tgpsu4qz	18	35	in	in	IN
cord-268139-tgpsu4qz	18	36	kilodaltons	kilodalton	NNS
cord-268139-tgpsu4qz	18	37	.	.	.
cord-268139-tgpsu4qz	19	1	More	more	RBR
cord-268139-tgpsu4qz	19	2	recently	recently	RB
cord-268139-tgpsu4qz	19	3	,	,	,
cord-268139-tgpsu4qz	19	4	nsps	nsp	NNS
cord-268139-tgpsu4qz	19	5	have	have	VBP
cord-268139-tgpsu4qz	19	6	been	be	VBN
cord-268139-tgpsu4qz	19	7	named	name	VBN
cord-268139-tgpsu4qz	19	8	based	base	VBN
cord-268139-tgpsu4qz	19	9	on	on	IN
cord-268139-tgpsu4qz	19	10	their	-PRON-	PRP$
cord-268139-tgpsu4qz	19	11	order	order	NN
cord-268139-tgpsu4qz	19	12	in	in	IN
cord-268139-tgpsu4qz	19	13	gene	gene	NN
cord-268139-tgpsu4qz	19	14	1	1	CD
cord-268139-tgpsu4qz	19	15	and	and	CC
cord-268139-tgpsu4qz	19	16	are	be	VBP
cord-268139-tgpsu4qz	19	17	numbered	number	VBN
cord-268139-tgpsu4qz	19	18	consecutively	consecutively	RB
cord-268139-tgpsu4qz	19	19	beginning	begin	VBG
cord-268139-tgpsu4qz	19	20	at	at	IN
cord-268139-tgpsu4qz	19	21	the	the	DT
cord-268139-tgpsu4qz	19	22	amino	amino	NN
cord-268139-tgpsu4qz	19	23	-	-	HYPH
cord-268139-tgpsu4qz	19	24	terminus	terminus	NN
cord-268139-tgpsu4qz	19	25	of	of	IN
cord-268139-tgpsu4qz	19	26	the	the	DT
cord-268139-tgpsu4qz	19	27	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	19	28	(	(	-LRB-
cord-268139-tgpsu4qz	19	29	i.e.	i.e.	FW
cord-268139-tgpsu4qz	19	30	,	,	,
cord-268139-tgpsu4qz	19	31	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	19	32	through	through	IN
cord-268139-tgpsu4qz	19	33	nsp16	nsp16	NNS
cord-268139-tgpsu4qz	19	34	)	)	-RRB-
cord-268139-tgpsu4qz	20	1	(	(	-LRB-
cord-268139-tgpsu4qz	20	2	Harcourt	Harcourt	NNP
cord-268139-tgpsu4qz	20	3	et	et	NNP
cord-268139-tgpsu4qz	20	4	al	al	NNP
cord-268139-tgpsu4qz	20	5	.	.	NNP
cord-268139-tgpsu4qz	20	6	,	,	,
cord-268139-tgpsu4qz	20	7	2004	2004	CD
cord-268139-tgpsu4qz	21	1	;	;	:
cord-268139-tgpsu4qz	21	2	Prentice	Prentice	NNP
cord-268139-tgpsu4qz	21	3	et	et	NNP
cord-268139-tgpsu4qz	21	4	al	al	NNP
cord-268139-tgpsu4qz	21	5	.	.	.
cord-268139-tgpsu4qz	22	1	,	,	,
cord-268139-tgpsu4qz	22	2	2004b	2004b	CD
cord-268139-tgpsu4qz	22	3	;	;	:
cord-268139-tgpsu4qz	22	4	Snijder	Snijder	NNP
cord-268139-tgpsu4qz	22	5	et	et	NNP
cord-268139-tgpsu4qz	22	6	al	al	NNP
cord-268139-tgpsu4qz	22	7	.	.	NNP
cord-268139-tgpsu4qz	22	8	,	,	,
cord-268139-tgpsu4qz	22	9	2003	2003	CD
cord-268139-tgpsu4qz	22	10	)	)	-RRB-
cord-268139-tgpsu4qz	22	11	.	.	.
cord-268139-tgpsu4qz	23	1	The	the	DT
cord-268139-tgpsu4qz	23	2	gene	gene	NN
cord-268139-tgpsu4qz	23	3	1	1	CD
cord-268139-tgpsu4qz	23	4	nsps	nsp	NNS
cord-268139-tgpsu4qz	23	5	of	of	IN
cord-268139-tgpsu4qz	23	6	the	the	DT
cord-268139-tgpsu4qz	23	7	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	23	8	murine	murine	JJ
cord-268139-tgpsu4qz	23	9	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	23	10	virus	virus	NN
cord-268139-tgpsu4qz	23	11	(	(	-LRB-
cord-268139-tgpsu4qz	23	12	MHV	MHV	NNP
cord-268139-tgpsu4qz	23	13	)	)	-RRB-
cord-268139-tgpsu4qz	23	14	are	be	VBP
cord-268139-tgpsu4qz	23	15	similar	similar	JJ
cord-268139-tgpsu4qz	23	16	in	in	IN
cord-268139-tgpsu4qz	23	17	number	number	NN
cord-268139-tgpsu4qz	23	18	,	,	,
cord-268139-tgpsu4qz	23	19	size	size	NN
cord-268139-tgpsu4qz	23	20	,	,	,
cord-268139-tgpsu4qz	23	21	and	and	CC
cord-268139-tgpsu4qz	23	22	organization	organization	NN
cord-268139-tgpsu4qz	23	23	to	to	IN
cord-268139-tgpsu4qz	23	24	those	those	DT
cord-268139-tgpsu4qz	23	25	of	of	IN
cord-268139-tgpsu4qz	23	26	SARS	SARS	NNP
cord-268139-tgpsu4qz	23	27	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	23	28	(	(	-LRB-
cord-268139-tgpsu4qz	23	29	SARS	SARS	NNP
cord-268139-tgpsu4qz	23	30	-	-	HYPH
cord-268139-tgpsu4qz	23	31	CoV	CoV	NNP
cord-268139-tgpsu4qz	23	32	)	)	-RRB-
cord-268139-tgpsu4qz	23	33	(	(	-LRB-
cord-268139-tgpsu4qz	23	34	Marra	Marra	NNP
cord-268139-tgpsu4qz	23	35	et	et	NNP
cord-268139-tgpsu4qz	23	36	al	al	NNP
cord-268139-tgpsu4qz	23	37	.	.	NNP
cord-268139-tgpsu4qz	23	38	,	,	,
cord-268139-tgpsu4qz	23	39	2003	2003	CD
cord-268139-tgpsu4qz	23	40	;	;	:
cord-268139-tgpsu4qz	23	41	Snijder	Snijder	NNP
cord-268139-tgpsu4qz	23	42	et	et	NNP
cord-268139-tgpsu4qz	23	43	al	al	NNP
cord-268139-tgpsu4qz	23	44	.	.	NNP
cord-268139-tgpsu4qz	23	45	,	,	,
cord-268139-tgpsu4qz	23	46	2003	2003	CD
cord-268139-tgpsu4qz	23	47	)	)	-RRB-
cord-268139-tgpsu4qz	23	48	.	.	.
cord-268139-tgpsu4qz	24	1	This	this	DT
cord-268139-tgpsu4qz	24	2	resemblance	resemblance	NN
cord-268139-tgpsu4qz	24	3	suggests	suggest	VBZ
cord-268139-tgpsu4qz	24	4	that	that	IN
cord-268139-tgpsu4qz	24	5	MHV	MHV	NNP
cord-268139-tgpsu4qz	24	6	is	be	VBZ
cord-268139-tgpsu4qz	24	7	an	an	DT
cord-268139-tgpsu4qz	24	8	excellent	excellent	JJ
cord-268139-tgpsu4qz	24	9	model	model	NN
cord-268139-tgpsu4qz	24	10	for	for	IN
cord-268139-tgpsu4qz	24	11	studies	study	NNS
cord-268139-tgpsu4qz	24	12	of	of	IN
cord-268139-tgpsu4qz	24	13	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	24	14	nsp	nsp	NN
cord-268139-tgpsu4qz	24	15	function	function	NN
cord-268139-tgpsu4qz	24	16	and	and	CC
cord-268139-tgpsu4qz	24	17	may	may	NNP
cord-268139-tgpsu4qz	24	18	0042	0042	CD
cord-268139-tgpsu4qz	24	19	-	-	:
cord-268139-tgpsu4qz	24	20	6822/$	6822/$	IN
cord-268139-tgpsu4qz	24	21	-see	-see	HYPH
cord-268139-tgpsu4qz	24	22	front	front	NN
cord-268139-tgpsu4qz	24	23	matter	matter	NN
cord-268139-tgpsu4qz	24	24	D	D	NNP
cord-268139-tgpsu4qz	25	1	2005	2005	CD
cord-268139-tgpsu4qz	25	2	Elsevier	Elsevier	NNP
cord-268139-tgpsu4qz	25	3	Inc.	Inc.	NNP
cord-268139-tgpsu4qz	25	4	All	all	DT
cord-268139-tgpsu4qz	25	5	rights	right	NNS
cord-268139-tgpsu4qz	25	6	reserved	reserve	VBN
cord-268139-tgpsu4qz	25	7	.	.	.
cord-268139-tgpsu4qz	26	1	doi:10.1016	doi:10.1016	RB
cord-268139-tgpsu4qz	27	1	/j.virol.2005.06.035	/j.virol.2005.06.035	NFP
cord-268139-tgpsu4qz	27	2	increase	increase	VB
cord-268139-tgpsu4qz	27	3	our	-PRON-	PRP$
cord-268139-tgpsu4qz	27	4	understanding	understanding	NN
cord-268139-tgpsu4qz	27	5	of	of	IN
cord-268139-tgpsu4qz	27	6	SARS	SARS	NNP
cord-268139-tgpsu4qz	27	7	-	-	HYPH
cord-268139-tgpsu4qz	27	8	CoV	cov	NN
cord-268139-tgpsu4qz	27	9	replication	replication	NN
cord-268139-tgpsu4qz	27	10	and	and	CC
cord-268139-tgpsu4qz	27	11	pathogenesis	pathogenesis	NN
cord-268139-tgpsu4qz	27	12	.	.	.
cord-268139-tgpsu4qz	28	1	Following	follow	VBG
cord-268139-tgpsu4qz	28	2	entry	entry	NN
cord-268139-tgpsu4qz	28	3	of	of	IN
cord-268139-tgpsu4qz	28	4	MHV	MHV	NNP
cord-268139-tgpsu4qz	28	5	into	into	IN
cord-268139-tgpsu4qz	28	6	a	a	DT
cord-268139-tgpsu4qz	28	7	host	host	NN
cord-268139-tgpsu4qz	28	8	cell	cell	NN
cord-268139-tgpsu4qz	28	9	,	,	,
cord-268139-tgpsu4qz	28	10	the	the	DT
cord-268139-tgpsu4qz	28	11	first	first	JJ
cord-268139-tgpsu4qz	28	12	event	event	NN
cord-268139-tgpsu4qz	28	13	in	in	IN
cord-268139-tgpsu4qz	28	14	the	the	DT
cord-268139-tgpsu4qz	28	15	virus	virus	NN
cord-268139-tgpsu4qz	28	16	life	life	NN
cord-268139-tgpsu4qz	28	17	cycle	cycle	NN
cord-268139-tgpsu4qz	28	18	is	be	VBZ
cord-268139-tgpsu4qz	28	19	translation	translation	NN
cord-268139-tgpsu4qz	28	20	of	of	IN
cord-268139-tgpsu4qz	28	21	two	two	CD
cord-268139-tgpsu4qz	28	22	polyproteins	polyprotein	NNS
cord-268139-tgpsu4qz	28	23	from	from	IN
cord-268139-tgpsu4qz	28	24	gene	gene	NN
cord-268139-tgpsu4qz	28	25	1	1	CD
cord-268139-tgpsu4qz	28	26	of	of	IN
cord-268139-tgpsu4qz	28	27	the	the	DT
cord-268139-tgpsu4qz	28	28	input	input	NN
cord-268139-tgpsu4qz	28	29	RNA	RNA	NNP
cord-268139-tgpsu4qz	28	30	genome	genome	NN
cord-268139-tgpsu4qz	28	31	.	.	.
cord-268139-tgpsu4qz	29	1	Gene	gene	NN
cord-268139-tgpsu4qz	29	2	1	1	CD
cord-268139-tgpsu4qz	29	3	is	be	VBZ
cord-268139-tgpsu4qz	29	4	comprised	comprise	VBN
cord-268139-tgpsu4qz	29	5	of	of	IN
cord-268139-tgpsu4qz	29	6	two	two	CD
cord-268139-tgpsu4qz	29	7	overlapping	overlap	VBG
cord-268139-tgpsu4qz	29	8	open	open	JJ
cord-268139-tgpsu4qz	29	9	reading	reading	NN
cord-268139-tgpsu4qz	29	10	frames	frame	NNS
cord-268139-tgpsu4qz	29	11	(	(	-LRB-
cord-268139-tgpsu4qz	29	12	ORF1a	ORF1a	NNP
cord-268139-tgpsu4qz	29	13	and	and	CC
cord-268139-tgpsu4qz	29	14	ORF1b	ORF1b	NNP
cord-268139-tgpsu4qz	29	15	)	)	-RRB-
cord-268139-tgpsu4qz	29	16	that	that	WDT
cord-268139-tgpsu4qz	29	17	are	be	VBP
cord-268139-tgpsu4qz	29	18	connected	connect	VBN
cord-268139-tgpsu4qz	29	19	by	by	IN
cord-268139-tgpsu4qz	29	20	a	a	DT
cord-268139-tgpsu4qz	29	21	À1	À1	NNP
cord-268139-tgpsu4qz	29	22	ribosomal	ribosomal	JJ
cord-268139-tgpsu4qz	29	23	frameshift	frameshift	NN
cord-268139-tgpsu4qz	29	24	(	(	-LRB-
cord-268139-tgpsu4qz	29	25	Fig	Fig	NNP
cord-268139-tgpsu4qz	29	26	.	.	.
cord-268139-tgpsu4qz	29	27	1A	1a	NN
cord-268139-tgpsu4qz	29	28	)	)	-RRB-
cord-268139-tgpsu4qz	30	1	(	(	-LRB-
cord-268139-tgpsu4qz	30	2	Bonilla	Bonilla	NNP
cord-268139-tgpsu4qz	30	3	et	et	NNP
cord-268139-tgpsu4qz	30	4	al	al	NNP
cord-268139-tgpsu4qz	30	5	.	.	.
cord-268139-tgpsu4qz	31	1	,	,	,
cord-268139-tgpsu4qz	31	2	1994	1994	CD
cord-268139-tgpsu4qz	31	3	;	;	:
cord-268139-tgpsu4qz	31	4	Bredenbeek	Bredenbeek	NNP
cord-268139-tgpsu4qz	31	5	et	et	NNP
cord-268139-tgpsu4qz	31	6	al	al	NNP
cord-268139-tgpsu4qz	31	7	.	.	NNP
cord-268139-tgpsu4qz	31	8	,	,	,
cord-268139-tgpsu4qz	31	9	1990	1990	CD
cord-268139-tgpsu4qz	31	10	;	;	:
cord-268139-tgpsu4qz	31	11	Brierley	Brierley	NNP
cord-268139-tgpsu4qz	31	12	et	et	NNP
cord-268139-tgpsu4qz	31	13	al	al	NNP
cord-268139-tgpsu4qz	31	14	.	.	NNP
cord-268139-tgpsu4qz	31	15	,	,	,
cord-268139-tgpsu4qz	31	16	1989	1989	CD
cord-268139-tgpsu4qz	32	1	;	;	:
cord-268139-tgpsu4qz	32	2	Lee	Lee	NNP
cord-268139-tgpsu4qz	32	3	et	et	NNP
cord-268139-tgpsu4qz	32	4	al	al	NNP
cord-268139-tgpsu4qz	32	5	.	.	NNP
cord-268139-tgpsu4qz	32	6	,	,	,
cord-268139-tgpsu4qz	32	7	1991	1991	CD
cord-268139-tgpsu4qz	32	8	;	;	:
cord-268139-tgpsu4qz	32	9	Pachuk	Pachuk	NNP
cord-268139-tgpsu4qz	32	10	et	et	NNP
cord-268139-tgpsu4qz	32	11	al	al	NNP
cord-268139-tgpsu4qz	32	12	.	.	NNP
cord-268139-tgpsu4qz	32	13	,	,	,
cord-268139-tgpsu4qz	32	14	1989	1989	CD
cord-268139-tgpsu4qz	32	15	)	)	-RRB-
cord-268139-tgpsu4qz	32	16	.	.	.
cord-268139-tgpsu4qz	33	1	Translation	translation	NN
cord-268139-tgpsu4qz	33	2	of	of	IN
cord-268139-tgpsu4qz	33	3	either	either	DT
cord-268139-tgpsu4qz	33	4	ORF1a	ORF1a	NNP
cord-268139-tgpsu4qz	33	5	or	or	CC
cord-268139-tgpsu4qz	33	6	the	the	DT
cord-268139-tgpsu4qz	33	7	ORF1ab	ORF1ab	NNP
cord-268139-tgpsu4qz	33	8	fusion	fusion	NN
cord-268139-tgpsu4qz	33	9	results	result	NNS
cord-268139-tgpsu4qz	33	10	in	in	IN
cord-268139-tgpsu4qz	33	11	possible	possible	JJ
cord-268139-tgpsu4qz	33	12	495-kDa	495-kda	CD
cord-268139-tgpsu4qz	33	13	or	or	CC
cord-268139-tgpsu4qz	33	14	803-kDa	803-kda	CD
cord-268139-tgpsu4qz	33	15	polyproteins	polyprotein	NNS
cord-268139-tgpsu4qz	33	16	,	,	,
cord-268139-tgpsu4qz	33	17	respectively	respectively	RB
cord-268139-tgpsu4qz	33	18	.	.	.
cord-268139-tgpsu4qz	34	1	These	these	DT
cord-268139-tgpsu4qz	34	2	co	co	JJ
cord-268139-tgpsu4qz	34	3	-	-	JJ
cord-268139-tgpsu4qz	34	4	aminoterminal	aminoterminal	JJ
cord-268139-tgpsu4qz	34	5	polyproteins	polyprotein	NNS
cord-268139-tgpsu4qz	34	6	are	be	VBP
cord-268139-tgpsu4qz	34	7	proteolytically	proteolytically	RB
cord-268139-tgpsu4qz	34	8	processed	process	VBN
cord-268139-tgpsu4qz	34	9	by	by	IN
cord-268139-tgpsu4qz	34	10	three	three	CD
cord-268139-tgpsu4qz	34	11	virus	virus	NN
cord-268139-tgpsu4qz	34	12	-	-	HYPH
cord-268139-tgpsu4qz	34	13	encoded	encode	VBN
cord-268139-tgpsu4qz	34	14	proteinases	proteinase	NNS
cord-268139-tgpsu4qz	34	15	,	,	,
cord-268139-tgpsu4qz	34	16	including	include	VBG
cord-268139-tgpsu4qz	34	17	two	two	CD
cord-268139-tgpsu4qz	34	18	papain	papain	NN
cord-268139-tgpsu4qz	34	19	-	-	HYPH
cord-268139-tgpsu4qz	34	20	like	like	IN
cord-268139-tgpsu4qz	34	21	proteinases	proteinase	NNS
cord-268139-tgpsu4qz	34	22	(	(	-LRB-
cord-268139-tgpsu4qz	34	23	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	34	24	and	and	CC
cord-268139-tgpsu4qz	34	25	PLP2	PLP2	NNP
cord-268139-tgpsu4qz	34	26	within	within	IN
cord-268139-tgpsu4qz	34	27	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	34	28	)	)	-RRB-
cord-268139-tgpsu4qz	34	29	and	and	CC
cord-268139-tgpsu4qz	34	30	a	a	DT
cord-268139-tgpsu4qz	34	31	picoronavirus	picoronavirus	JJ
cord-268139-tgpsu4qz	34	32	3C	3c	NN
cord-268139-tgpsu4qz	34	33	-	-	HYPH
cord-268139-tgpsu4qz	34	34	like	like	JJ
cord-268139-tgpsu4qz	34	35	proteinase	proteinase	NN
cord-268139-tgpsu4qz	34	36	(	(	-LRB-
cord-268139-tgpsu4qz	34	37	nsp5	nsp5	NN
cord-268139-tgpsu4qz	34	38	)	)	-RRB-
cord-268139-tgpsu4qz	34	39	,	,	,
cord-268139-tgpsu4qz	34	40	to	to	TO
cord-268139-tgpsu4qz	34	41	yield	yield	VB
cord-268139-tgpsu4qz	34	42	at	at	RB
cord-268139-tgpsu4qz	34	43	least	least	RBS
cord-268139-tgpsu4qz	34	44	16	16	CD
cord-268139-tgpsu4qz	34	45	mature	mature	JJ
cord-268139-tgpsu4qz	34	46	gene	gene	NN
cord-268139-tgpsu4qz	34	47	1	1	CD
cord-268139-tgpsu4qz	34	48	nsps	nsp	NNS
cord-268139-tgpsu4qz	34	49	as	as	RB
cord-268139-tgpsu4qz	34	50	well	well	RB
cord-268139-tgpsu4qz	34	51	as	as	IN
cord-268139-tgpsu4qz	34	52	intermediate	intermediate	JJ
cord-268139-tgpsu4qz	34	53	precursors	precursor	NNS
cord-268139-tgpsu4qz	34	54	.	.	.
cord-268139-tgpsu4qz	35	1	To	to	IN
cord-268139-tgpsu4qz	35	2	date	date	NN
cord-268139-tgpsu4qz	35	3	,	,	,
cord-268139-tgpsu4qz	35	4	all	all	DT
cord-268139-tgpsu4qz	35	5	MHV	MHV	NNP
cord-268139-tgpsu4qz	35	6	gene	gene	NN
cord-268139-tgpsu4qz	35	7	1	1	CD
cord-268139-tgpsu4qz	35	8	nsps	nsp	NNS
cord-268139-tgpsu4qz	35	9	tested	test	VBN
cord-268139-tgpsu4qz	35	10	co	co	NN
cord-268139-tgpsu4qz	35	11	-	-	NN
cord-268139-tgpsu4qz	35	12	localize	localize	VB
cord-268139-tgpsu4qz	35	13	with	with	IN
cord-268139-tgpsu4qz	35	14	sites	site	NNS
cord-268139-tgpsu4qz	35	15	of	of	IN
cord-268139-tgpsu4qz	35	16	active	active	JJ
cord-268139-tgpsu4qz	35	17	viral	viral	JJ
cord-268139-tgpsu4qz	35	18	RNA	RNA	NNP
cord-268139-tgpsu4qz	35	19	synthesis	synthesis	NN
cord-268139-tgpsu4qz	35	20	at	at	IN
cord-268139-tgpsu4qz	35	21	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	35	22	viral	viral	JJ
cord-268139-tgpsu4qz	35	23	replication	replication	NN
cord-268139-tgpsu4qz	35	24	complexes	complex	NNS
cord-268139-tgpsu4qz	35	25	on	on	IN
cord-268139-tgpsu4qz	35	26	intracellular	intracellular	JJ
cord-268139-tgpsu4qz	35	27	double	double	JJ
cord-268139-tgpsu4qz	35	28	-	-	HYPH
cord-268139-tgpsu4qz	35	29	membrane	membrane	NN
cord-268139-tgpsu4qz	35	30	vesicles	vesicle	NNS
cord-268139-tgpsu4qz	35	31	(	(	-LRB-
cord-268139-tgpsu4qz	35	32	Bost	Bost	NNP
cord-268139-tgpsu4qz	35	33	et	et	NNP
cord-268139-tgpsu4qz	35	34	al	al	NNP
cord-268139-tgpsu4qz	35	35	.	.	NNP
cord-268139-tgpsu4qz	35	36	,	,	,
cord-268139-tgpsu4qz	35	37	2000	2000	CD
cord-268139-tgpsu4qz	35	38	(	(	-LRB-
cord-268139-tgpsu4qz	35	39	Bost	Bost	NNP
cord-268139-tgpsu4qz	35	40	et	et	NNP
cord-268139-tgpsu4qz	35	41	al	al	NNP
cord-268139-tgpsu4qz	35	42	.	.	NNP
cord-268139-tgpsu4qz	35	43	,	,	,
cord-268139-tgpsu4qz	35	44	,	,	,
cord-268139-tgpsu4qz	35	45	2001	2001	CD
cord-268139-tgpsu4qz	35	46	Brockway	Brockway	NNP
cord-268139-tgpsu4qz	35	47	et	et	NNP
cord-268139-tgpsu4qz	35	48	al	al	NNP
cord-268139-tgpsu4qz	35	49	.	.	NNP
cord-268139-tgpsu4qz	35	50	,	,	,
cord-268139-tgpsu4qz	35	51	2003	2003	CD
cord-268139-tgpsu4qz	35	52	Brockway	Brockway	NNP
cord-268139-tgpsu4qz	35	53	et	et	NNP
cord-268139-tgpsu4qz	35	54	al	al	NNP
cord-268139-tgpsu4qz	35	55	.	.	NNP
cord-268139-tgpsu4qz	35	56	,	,	,
cord-268139-tgpsu4qz	35	57	,	,	,
cord-268139-tgpsu4qz	35	58	2004	2004	CD
cord-268139-tgpsu4qz	35	59	Gosert	Gosert	NNP
cord-268139-tgpsu4qz	35	60	et	et	NNP
cord-268139-tgpsu4qz	35	61	al	al	NNP
cord-268139-tgpsu4qz	35	62	.	.	NNP
cord-268139-tgpsu4qz	35	63	,	,	,
cord-268139-tgpsu4qz	35	64	2002	2002	CD
cord-268139-tgpsu4qz	35	65	;	;	:
cord-268139-tgpsu4qz	35	66	Prentice	Prentice	NNP
cord-268139-tgpsu4qz	35	67	et	et	NNP
cord-268139-tgpsu4qz	35	68	al	al	NNP
cord-268139-tgpsu4qz	35	69	.	.	NNP
cord-268139-tgpsu4qz	35	70	,	,	,
cord-268139-tgpsu4qz	35	71	2004a	2004a	CD
cord-268139-tgpsu4qz	35	72	;	;	:
cord-268139-tgpsu4qz	35	73	Shi	Shi	NNP
cord-268139-tgpsu4qz	35	74	et	et	FW
cord-268139-tgpsu4qz	35	75	al	al	NNP
cord-268139-tgpsu4qz	35	76	.	.	NNP
cord-268139-tgpsu4qz	35	77	,	,	,
cord-268139-tgpsu4qz	35	78	1999	1999	CD
cord-268139-tgpsu4qz	35	79	;	;	:
cord-268139-tgpsu4qz	35	80	van	van	NNP
cord-268139-tgpsu4qz	35	81	der	der	NNP
cord-268139-tgpsu4qz	35	82	Meer	Meer	NNP
cord-268139-tgpsu4qz	35	83	et	et	NNP
cord-268139-tgpsu4qz	35	84	al	al	NNP
cord-268139-tgpsu4qz	35	85	.	.	NNP
cord-268139-tgpsu4qz	35	86	,	,	,
cord-268139-tgpsu4qz	35	87	1999	1999	CD
cord-268139-tgpsu4qz	35	88	)	)	-RRB-
cord-268139-tgpsu4qz	35	89	.	.	.
cord-268139-tgpsu4qz	36	1	Nsps	Nsps	NNP
cord-268139-tgpsu4qz	36	2	are	be	VBP
cord-268139-tgpsu4qz	36	3	thought	think	VBN
cord-268139-tgpsu4qz	36	4	to	to	TO
cord-268139-tgpsu4qz	36	5	mediate	mediate	VB
cord-268139-tgpsu4qz	36	6	replication	replication	NN
cord-268139-tgpsu4qz	36	7	of	of	IN
cord-268139-tgpsu4qz	36	8	the	the	DT
cord-268139-tgpsu4qz	36	9	MHV	MHV	NNP
cord-268139-tgpsu4qz	36	10	RNA	RNA	NNP
cord-268139-tgpsu4qz	36	11	genome	genome	NN
cord-268139-tgpsu4qz	36	12	and	and	CC
cord-268139-tgpsu4qz	36	13	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	36	14	RNA	RNA	NNP
cord-268139-tgpsu4qz	36	15	synthesis	synthesis	NN
cord-268139-tgpsu4qz	36	16	at	at	IN
cord-268139-tgpsu4qz	36	17	these	these	DT
cord-268139-tgpsu4qz	36	18	membrane	membrane	NN
cord-268139-tgpsu4qz	36	19	-	-	HYPH
cord-268139-tgpsu4qz	36	20	bound	bind	VBN
cord-268139-tgpsu4qz	36	21	complexes	complex	NNS
cord-268139-tgpsu4qz	36	22	.	.	.
cord-268139-tgpsu4qz	37	1	However	however	RB
cord-268139-tgpsu4qz	37	2	,	,	,
cord-268139-tgpsu4qz	37	3	the	the	DT
cord-268139-tgpsu4qz	37	4	viral	viral	JJ
cord-268139-tgpsu4qz	37	5	proteinases	proteinase	NNS
cord-268139-tgpsu4qz	37	6	are	be	VBP
cord-268139-tgpsu4qz	37	7	the	the	DT
cord-268139-tgpsu4qz	37	8	only	only	JJ
cord-268139-tgpsu4qz	37	9	MHV	MHV	NNP
cord-268139-tgpsu4qz	37	10	nsps	nsp	NNS
cord-268139-tgpsu4qz	37	11	with	with	IN
cord-268139-tgpsu4qz	37	12	experimentally	experimentally	RB
cord-268139-tgpsu4qz	37	13	confirmed	confirm	VBN
cord-268139-tgpsu4qz	37	14	functions	function	NNS
cord-268139-tgpsu4qz	37	15	(	(	-LRB-
cord-268139-tgpsu4qz	37	16	Baker	Baker	NNP
cord-268139-tgpsu4qz	37	17	et	et	NNP
cord-268139-tgpsu4qz	37	18	al	al	NNP
cord-268139-tgpsu4qz	37	19	.	.	NNP
cord-268139-tgpsu4qz	37	20	,	,	,
cord-268139-tgpsu4qz	37	21	1989	1989	CD
cord-268139-tgpsu4qz	37	22	;	;	:
cord-268139-tgpsu4qz	37	23	Bonilla	Bonilla	NNP
cord-268139-tgpsu4qz	37	24	et	et	NNP
cord-268139-tgpsu4qz	37	25	al	al	NNP
cord-268139-tgpsu4qz	37	26	.	.	NNP
cord-268139-tgpsu4qz	37	27	,	,	,
cord-268139-tgpsu4qz	37	28	1995	1995	CD
cord-268139-tgpsu4qz	37	29	;	;	:
cord-268139-tgpsu4qz	37	30	Lu	Lu	NNP
cord-268139-tgpsu4qz	37	31	et	et	FW
cord-268139-tgpsu4qz	37	32	al	al	NNP
cord-268139-tgpsu4qz	37	33	.	.	NNP
cord-268139-tgpsu4qz	37	34	,	,	,
cord-268139-tgpsu4qz	37	35	1996	1996	CD
cord-268139-tgpsu4qz	37	36	;	;	:
cord-268139-tgpsu4qz	37	37	Lu	Lu	NNP
cord-268139-tgpsu4qz	37	38	et	et	FW
cord-268139-tgpsu4qz	37	39	al	al	NNP
cord-268139-tgpsu4qz	37	40	.	.	NNP
cord-268139-tgpsu4qz	37	41	,	,	,
cord-268139-tgpsu4qz	37	42	1995	1995	CD
cord-268139-tgpsu4qz	37	43	)	)	-RRB-
cord-268139-tgpsu4qz	37	44	.	.	.
cord-268139-tgpsu4qz	38	1	Based	base	VBN
cord-268139-tgpsu4qz	38	2	on	on	IN
cord-268139-tgpsu4qz	38	3	homology	homology	NN
cord-268139-tgpsu4qz	38	4	to	to	IN
cord-268139-tgpsu4qz	38	5	proteins	protein	NNS
cord-268139-tgpsu4qz	38	6	with	with	IN
cord-268139-tgpsu4qz	38	7	known	know	VBN
cord-268139-tgpsu4qz	38	8	functions	function	NNS
cord-268139-tgpsu4qz	38	9	,	,	,
cord-268139-tgpsu4qz	38	10	roles	role	NNS
cord-268139-tgpsu4qz	38	11	in	in	IN
cord-268139-tgpsu4qz	38	12	viral	viral	JJ
cord-268139-tgpsu4qz	38	13	RNA	RNA	NNP
cord-268139-tgpsu4qz	38	14	synthesis	synthesis	NN
cord-268139-tgpsu4qz	38	15	have	have	VBP
cord-268139-tgpsu4qz	38	16	been	be	VBN
cord-268139-tgpsu4qz	38	17	predicted	predict	VBN
cord-268139-tgpsu4qz	38	18	for	for	IN
cord-268139-tgpsu4qz	38	19	several	several	JJ
cord-268139-tgpsu4qz	38	20	other	other	JJ
cord-268139-tgpsu4qz	38	21	MHV	MHV	NNP
cord-268139-tgpsu4qz	38	22	gene	gene	NN
cord-268139-tgpsu4qz	38	23	1	1	CD
cord-268139-tgpsu4qz	38	24	nsps	nsp	NNS
cord-268139-tgpsu4qz	38	25	.	.	.
cord-268139-tgpsu4qz	39	1	These	these	DT
cord-268139-tgpsu4qz	39	2	proteins	protein	NNS
cord-268139-tgpsu4qz	39	3	include	include	VBP
cord-268139-tgpsu4qz	39	4	two	two	CD
cord-268139-tgpsu4qz	39	5	trans	trans	JJ
cord-268139-tgpsu4qz	39	6	-	-	NN
cord-268139-tgpsu4qz	39	7	membrane	membrane	NN
cord-268139-tgpsu4qz	39	8	scaffolding	scaffolding	NN
cord-268139-tgpsu4qz	39	9	proteins	protein	NNS
cord-268139-tgpsu4qz	39	10	(	(	-LRB-
cord-268139-tgpsu4qz	39	11	nsp4	nsp4	NNS
cord-268139-tgpsu4qz	39	12	and	and	CC
cord-268139-tgpsu4qz	39	13	nsp6	nsp6	NN
cord-268139-tgpsu4qz	39	14	)	)	-RRB-
cord-268139-tgpsu4qz	39	15	(	(	-LRB-
cord-268139-tgpsu4qz	39	16	Gosert	Gosert	NNP
cord-268139-tgpsu4qz	39	17	et	et	NNP
cord-268139-tgpsu4qz	39	18	al	al	NNP
cord-268139-tgpsu4qz	39	19	.	.	NNP
cord-268139-tgpsu4qz	39	20	,	,	,
cord-268139-tgpsu4qz	39	21	2002	2002	CD
cord-268139-tgpsu4qz	39	22	)	)	-RRB-
cord-268139-tgpsu4qz	39	23	,	,	,
cord-268139-tgpsu4qz	39	24	an	an	DT
cord-268139-tgpsu4qz	39	25	RNA	RNA	NNP
cord-268139-tgpsu4qz	39	26	-	-	HYPH
cord-268139-tgpsu4qz	39	27	dependent	dependent	JJ
cord-268139-tgpsu4qz	39	28	RNA	RNA	NNP
cord-268139-tgpsu4qz	39	29	polymerase	polymerase	NN
cord-268139-tgpsu4qz	39	30	(	(	-LRB-
cord-268139-tgpsu4qz	39	31	nsp12	nsp12	CD
cord-268139-tgpsu4qz	39	32	)	)	-RRB-
cord-268139-tgpsu4qz	39	33	(	(	-LRB-
cord-268139-tgpsu4qz	39	34	Cheng	Cheng	NNP
cord-268139-tgpsu4qz	39	35	et	et	NNP
cord-268139-tgpsu4qz	39	36	al	al	NNP
cord-268139-tgpsu4qz	39	37	.	.	NNP
cord-268139-tgpsu4qz	39	38	,	,	,
cord-268139-tgpsu4qz	39	39	2005	2005	CD
cord-268139-tgpsu4qz	39	40	;	;	:
cord-268139-tgpsu4qz	39	41	Gorbalenya	Gorbalenya	NNP
cord-268139-tgpsu4qz	39	42	et	et	NNP
cord-268139-tgpsu4qz	39	43	al	al	NNP
cord-268139-tgpsu4qz	39	44	.	.	NNP
cord-268139-tgpsu4qz	39	45	,	,	,
cord-268139-tgpsu4qz	39	46	1989	1989	CD
cord-268139-tgpsu4qz	39	47	;	;	:
cord-268139-tgpsu4qz	39	48	Lee	Lee	NNP
cord-268139-tgpsu4qz	39	49	et	et	NNP
cord-268139-tgpsu4qz	39	50	al	al	NNP
cord-268139-tgpsu4qz	39	51	.	.	NNP
cord-268139-tgpsu4qz	39	52	,	,	,
cord-268139-tgpsu4qz	39	53	1991	1991	CD
cord-268139-tgpsu4qz	39	54	)	)	-RRB-
cord-268139-tgpsu4qz	39	55	,	,	,
cord-268139-tgpsu4qz	39	56	an	an	DT
cord-268139-tgpsu4qz	39	57	RNA	RNA	NNP
cord-268139-tgpsu4qz	39	58	helicase	helicase	NN
cord-268139-tgpsu4qz	39	59	(	(	-LRB-
cord-268139-tgpsu4qz	39	60	nsp13	nsp13	CD
cord-268139-tgpsu4qz	39	61	)	)	-RRB-
cord-268139-tgpsu4qz	40	1	(	(	-LRB-
cord-268139-tgpsu4qz	40	2	Seybert	Seybert	NNP
cord-268139-tgpsu4qz	40	3	and	and	CC
cord-268139-tgpsu4qz	40	4	Ziebuhr	Ziebuhr	NNP
cord-268139-tgpsu4qz	40	5	,	,	,
cord-268139-tgpsu4qz	40	6	2001	2001	CD
cord-268139-tgpsu4qz	40	7	;	;	:
cord-268139-tgpsu4qz	40	8	Seybert	Seybert	NNP
cord-268139-tgpsu4qz	40	9	et	et	NNP
cord-268139-tgpsu4qz	40	10	al	al	NNP
cord-268139-tgpsu4qz	40	11	.	.	NNP
cord-268139-tgpsu4qz	40	12	,	,	,
cord-268139-tgpsu4qz	40	13	2000	2000	CD
cord-268139-tgpsu4qz	40	14	)	)	-RRB-
cord-268139-tgpsu4qz	40	15	,	,	,
cord-268139-tgpsu4qz	40	16	and	and	CC
cord-268139-tgpsu4qz	40	17	several	several	JJ
cord-268139-tgpsu4qz	40	18	RNA	RNA	NNP
cord-268139-tgpsu4qz	40	19	processing	processing	NN
cord-268139-tgpsu4qz	40	20	enzymes	enzyme	NNS
cord-268139-tgpsu4qz	40	21	(	(	-LRB-
cord-268139-tgpsu4qz	41	1	nsp14	nsp14	UH
cord-268139-tgpsu4qz	41	2	,	,	,
cord-268139-tgpsu4qz	41	3	nsp15	nsp15	NNP
cord-268139-tgpsu4qz	41	4	,	,	,
cord-268139-tgpsu4qz	41	5	and	and	CC
cord-268139-tgpsu4qz	41	6	nsp16	nsp16	NNS
cord-268139-tgpsu4qz	41	7	)	)	-RRB-
cord-268139-tgpsu4qz	42	1	(	(	-LRB-
cord-268139-tgpsu4qz	42	2	Bhardwaj	Bhardwaj	NNP
cord-268139-tgpsu4qz	42	3	et	et	NNP
cord-268139-tgpsu4qz	42	4	al	al	NNP
cord-268139-tgpsu4qz	42	5	.	.	NNP
cord-268139-tgpsu4qz	42	6	,	,	,
cord-268139-tgpsu4qz	42	7	2004	2004	CD
cord-268139-tgpsu4qz	43	1	;	;	:
cord-268139-tgpsu4qz	43	2	Ivanov	Ivanov	NNP
cord-268139-tgpsu4qz	43	3	et	et	NNP
cord-268139-tgpsu4qz	43	4	al	al	NNP
cord-268139-tgpsu4qz	43	5	.	.	NNP
cord-268139-tgpsu4qz	43	6	,	,	,
cord-268139-tgpsu4qz	43	7	2004	2004	CD
cord-268139-tgpsu4qz	43	8	;	;	:
cord-268139-tgpsu4qz	43	9	Thiel	Thiel	NNP
cord-268139-tgpsu4qz	43	10	et	et	NNP
cord-268139-tgpsu4qz	43	11	al	al	NNP
cord-268139-tgpsu4qz	43	12	.	.	.
cord-268139-tgpsu4qz	44	1	,	,	,
cord-268139-tgpsu4qz	44	2	2003	2003	CD
cord-268139-tgpsu4qz	44	3	;	;	:
cord-268139-tgpsu4qz	44	4	Ziebuhr	Ziebuhr	NNP
cord-268139-tgpsu4qz	44	5	,	,	,
cord-268139-tgpsu4qz	44	6	2005	2005	CD
cord-268139-tgpsu4qz	44	7	)	)	-RRB-
cord-268139-tgpsu4qz	44	8	.	.	.
cord-268139-tgpsu4qz	45	1	Currently	currently	RB
cord-268139-tgpsu4qz	45	2	,	,	,
cord-268139-tgpsu4qz	45	3	there	there	EX
cord-268139-tgpsu4qz	45	4	are	be	VBP
cord-268139-tgpsu4qz	45	5	no	no	DT
cord-268139-tgpsu4qz	45	6	known	know	VBN
cord-268139-tgpsu4qz	45	7	or	or	CC
cord-268139-tgpsu4qz	45	8	envisaged	envisage	VBN
cord-268139-tgpsu4qz	45	9	functions	function	NNS
cord-268139-tgpsu4qz	45	10	in	in	IN
cord-268139-tgpsu4qz	45	11	replication	replication	NN
cord-268139-tgpsu4qz	45	12	for	for	IN
cord-268139-tgpsu4qz	45	13	at	at	RB
cord-268139-tgpsu4qz	45	14	least	least	RBS
cord-268139-tgpsu4qz	45	15	seven	seven	CD
cord-268139-tgpsu4qz	45	16	MHV	MHV	NNP
cord-268139-tgpsu4qz	45	17	gene	gene	NN
cord-268139-tgpsu4qz	45	18	1	1	CD
cord-268139-tgpsu4qz	45	19	proteins	protein	NNS
cord-268139-tgpsu4qz	45	20	(	(	-LRB-
cord-268139-tgpsu4qz	45	21	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	45	22	,	,	,
cord-268139-tgpsu4qz	45	23	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	45	24	,	,	,
cord-268139-tgpsu4qz	45	25	and	and	CC
cord-268139-tgpsu4qz	45	26	nsp7	nsp7	NNP
cord-268139-tgpsu4qz	45	27	-11	-11	VBN
cord-268139-tgpsu4qz	45	28	)	)	-RRB-
cord-268139-tgpsu4qz	45	29	.	.	.
cord-268139-tgpsu4qz	46	1	Previous	previous	JJ
cord-268139-tgpsu4qz	46	2	studies	study	NNS
cord-268139-tgpsu4qz	46	3	have	have	VBP
cord-268139-tgpsu4qz	46	4	provided	provide	VBN
cord-268139-tgpsu4qz	46	5	intriguing	intriguing	JJ
cord-268139-tgpsu4qz	46	6	evidence	evidence	NN
cord-268139-tgpsu4qz	46	7	about	about	IN
cord-268139-tgpsu4qz	46	8	the	the	DT
cord-268139-tgpsu4qz	46	9	potential	potential	JJ
cord-268139-tgpsu4qz	46	10	functions	function	NNS
cord-268139-tgpsu4qz	46	11	of	of	IN
cord-268139-tgpsu4qz	46	12	the	the	DT
cord-268139-tgpsu4qz	46	13	28-kDa	28-kda	CD
cord-268139-tgpsu4qz	46	14	amino	amino	NN
cord-268139-tgpsu4qz	46	15	-	-	HYPH
cord-268139-tgpsu4qz	46	16	terminal	terminal	NN
cord-268139-tgpsu4qz	46	17	protein	protein	NN
cord-268139-tgpsu4qz	46	18	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	46	19	(	(	-LRB-
cord-268139-tgpsu4qz	46	20	previously	previously	RB
cord-268139-tgpsu4qz	46	21	referred	refer	VBN
cord-268139-tgpsu4qz	46	22	to	to	IN
cord-268139-tgpsu4qz	46	23	as	as	IN
cord-268139-tgpsu4qz	46	24	p28	p28	CD
cord-268139-tgpsu4qz	46	25	)	)	-RRB-
cord-268139-tgpsu4qz	46	26	in	in	IN
cord-268139-tgpsu4qz	46	27	MHV	MHV	NNP
cord-268139-tgpsu4qz	46	28	replication	replication	NN
cord-268139-tgpsu4qz	46	29	.	.	.
cord-268139-tgpsu4qz	47	1	The	the	DT
cord-268139-tgpsu4qz	47	2	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	47	3	of	of	IN
cord-268139-tgpsu4qz	47	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	47	5	expression	expression	NN
cord-268139-tgpsu4qz	47	6	suggest	suggest	VBP
cord-268139-tgpsu4qz	47	7	that	that	IN
cord-268139-tgpsu4qz	47	8	it	-PRON-	PRP
cord-268139-tgpsu4qz	47	9	might	may	MD
cord-268139-tgpsu4qz	47	10	have	have	VB
cord-268139-tgpsu4qz	47	11	an	an	DT
cord-268139-tgpsu4qz	47	12	early	early	JJ
cord-268139-tgpsu4qz	47	13	regulatory	regulatory	JJ
cord-268139-tgpsu4qz	47	14	role	role	NN
cord-268139-tgpsu4qz	47	15	during	during	IN
cord-268139-tgpsu4qz	47	16	the	the	DT
cord-268139-tgpsu4qz	47	17	viral	viral	JJ
cord-268139-tgpsu4qz	47	18	life	life	NN
cord-268139-tgpsu4qz	47	19	cycle	cycle	NN
cord-268139-tgpsu4qz	47	20	.	.	.
cord-268139-tgpsu4qz	48	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	48	2	is	be	VBZ
cord-268139-tgpsu4qz	48	3	the	the	DT
cord-268139-tgpsu4qz	48	4	first	first	JJ
cord-268139-tgpsu4qz	48	5	mature	mature	JJ
cord-268139-tgpsu4qz	48	6	protein	protein	NN
cord-268139-tgpsu4qz	48	7	processed	process	VBN
cord-268139-tgpsu4qz	48	8	from	from	IN
cord-268139-tgpsu4qz	48	9	the	the	DT
cord-268139-tgpsu4qz	48	10	gene	gene	NN
cord-268139-tgpsu4qz	48	11	1	1	CD
cord-268139-tgpsu4qz	48	12	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	48	13	and	and	CC
cord-268139-tgpsu4qz	48	14	is	be	VBZ
cord-268139-tgpsu4qz	48	15	likely	likely	RB
cord-268139-tgpsu4qz	48	16	cleaved	cleave	VBN
cord-268139-tgpsu4qz	48	17	quickly	quickly	RB
cord-268139-tgpsu4qz	48	18	following	follow	VBG
cord-268139-tgpsu4qz	48	19	trans-	trans-	XX
cord-268139-tgpsu4qz	49	1	ORF1a	ORF1a	NNP
cord-268139-tgpsu4qz	49	2	-ORF1b	-ORF1b	NNP
cord-268139-tgpsu4qz	49	3	fusion	fusion	NN
cord-268139-tgpsu4qz	49	4	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	49	5	is	be	VBZ
cord-268139-tgpsu4qz	49	6	illustrated	illustrate	VBN
cord-268139-tgpsu4qz	49	7	with	with	IN
cord-268139-tgpsu4qz	49	8	mature	mature	JJ
cord-268139-tgpsu4qz	49	9	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	49	10	protein	protein	NN
cord-268139-tgpsu4qz	49	11	(	(	-LRB-
cord-268139-tgpsu4qz	49	12	nsps	nsps	NNP
cord-268139-tgpsu4qz	49	13	)	)	-RRB-
cord-268139-tgpsu4qz	49	14	represented	represent	VBD
cord-268139-tgpsu4qz	49	15	as	as	IN
cord-268139-tgpsu4qz	49	16	numbered	number	VBN
cord-268139-tgpsu4qz	49	17	boxes	box	NNS
cord-268139-tgpsu4qz	49	18	.	.	.
cord-268139-tgpsu4qz	50	1	The	the	DT
cord-268139-tgpsu4qz	50	2	gray	gray	JJ
cord-268139-tgpsu4qz	50	3	box	box	NN
cord-268139-tgpsu4qz	50	4	represents	represent	VBZ
cord-268139-tgpsu4qz	50	5	the	the	DT
cord-268139-tgpsu4qz	50	6	aminoterminal	aminoterminal	JJ
cord-268139-tgpsu4qz	50	7	cleavage	cleavage	NN
cord-268139-tgpsu4qz	50	8	product	product	NN
cord-268139-tgpsu4qz	50	9	(	(	-LRB-
cord-268139-tgpsu4qz	50	10	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	50	11	)	)	-RRB-
cord-268139-tgpsu4qz	50	12	.	.	.
cord-268139-tgpsu4qz	51	1	Nsps	Nsps	NNP
cord-268139-tgpsu4qz	51	2	with	with	IN
cord-268139-tgpsu4qz	51	3	confirmed	confirm	VBN
cord-268139-tgpsu4qz	51	4	or	or	CC
cord-268139-tgpsu4qz	51	5	predicted	predict	VBN
cord-268139-tgpsu4qz	51	6	functions	function	NNS
cord-268139-tgpsu4qz	51	7	include	include	VBP
cord-268139-tgpsu4qz	51	8	:	:	:
cord-268139-tgpsu4qz	51	9	two	two	CD
cord-268139-tgpsu4qz	51	10	papain	papain	NN
cord-268139-tgpsu4qz	51	11	-	-	HYPH
cord-268139-tgpsu4qz	51	12	like	like	IN
cord-268139-tgpsu4qz	51	13	proteinases	proteinase	NNS
cord-268139-tgpsu4qz	51	14	(	(	-LRB-
cord-268139-tgpsu4qz	51	15	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	51	16	and	and	CC
cord-268139-tgpsu4qz	51	17	PLP2	PLP2	NNP
cord-268139-tgpsu4qz	51	18	within	within	IN
cord-268139-tgpsu4qz	51	19	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	51	20	)	)	-RRB-
cord-268139-tgpsu4qz	51	21	,	,	,
cord-268139-tgpsu4qz	51	22	the	the	DT
cord-268139-tgpsu4qz	51	23	3C	3c	NN
cord-268139-tgpsu4qz	51	24	-	-	HYPH
cord-268139-tgpsu4qz	51	25	like	like	JJ
cord-268139-tgpsu4qz	51	26	proteinase	proteinase	NN
cord-268139-tgpsu4qz	51	27	(	(	-LRB-
cord-268139-tgpsu4qz	51	28	3CL	3CL	NNP
cord-268139-tgpsu4qz	51	29	pro	pro	JJ
cord-268139-tgpsu4qz	51	30	;	;	:
cord-268139-tgpsu4qz	51	31	nsp5	nsp5	NNP
cord-268139-tgpsu4qz	51	32	)	)	-RRB-
cord-268139-tgpsu4qz	51	33	,	,	,
cord-268139-tgpsu4qz	51	34	two	two	CD
cord-268139-tgpsu4qz	51	35	trans	trans	NN
cord-268139-tgpsu4qz	51	36	-	-	NN
cord-268139-tgpsu4qz	51	37	membrane	membrane	NN
cord-268139-tgpsu4qz	51	38	proteins	protein	NNS
cord-268139-tgpsu4qz	51	39	(	(	-LRB-
cord-268139-tgpsu4qz	51	40	MP1	MP1	NNP
cord-268139-tgpsu4qz	51	41	and	and	CC
cord-268139-tgpsu4qz	51	42	MP2	MP2	NNP
cord-268139-tgpsu4qz	51	43	;	;	:
cord-268139-tgpsu4qz	51	44	nsp4	nsp4	NNP
cord-268139-tgpsu4qz	51	45	and	and	CC
cord-268139-tgpsu4qz	51	46	nsp6	nsp6	NN
cord-268139-tgpsu4qz	51	47	,	,	,
cord-268139-tgpsu4qz	51	48	respectively	respectively	RB
cord-268139-tgpsu4qz	51	49	)	)	-RRB-
cord-268139-tgpsu4qz	51	50	,	,	,
cord-268139-tgpsu4qz	51	51	the	the	DT
cord-268139-tgpsu4qz	51	52	RNA	RNA	NNP
cord-268139-tgpsu4qz	51	53	-	-	HYPH
cord-268139-tgpsu4qz	51	54	dependent	dependent	JJ
cord-268139-tgpsu4qz	51	55	RNA	RNA	NNP
cord-268139-tgpsu4qz	51	56	polymerase	polymerase	NN
cord-268139-tgpsu4qz	51	57	(	(	-LRB-
cord-268139-tgpsu4qz	51	58	Pol	Pol	NNP
cord-268139-tgpsu4qz	51	59	;	;	:
cord-268139-tgpsu4qz	51	60	nsp12	nsp12	ADD
cord-268139-tgpsu4qz	51	61	)	)	-RRB-
cord-268139-tgpsu4qz	51	62	,	,	,
cord-268139-tgpsu4qz	51	63	the	the	DT
cord-268139-tgpsu4qz	51	64	RNA	RNA	NNP
cord-268139-tgpsu4qz	51	65	helicase	helicase	NN
cord-268139-tgpsu4qz	51	66	(	(	-LRB-
cord-268139-tgpsu4qz	51	67	Hel	Hel	NNP
cord-268139-tgpsu4qz	51	68	;	;	:
cord-268139-tgpsu4qz	51	69	nsp13	nsp13	ADD
cord-268139-tgpsu4qz	51	70	)	)	-RRB-
cord-268139-tgpsu4qz	51	71	,	,	,
cord-268139-tgpsu4qz	51	72	the	the	DT
cord-268139-tgpsu4qz	51	73	3V	3v	NN
cord-268139-tgpsu4qz	51	74	-	-	HYPH
cord-268139-tgpsu4qz	51	75	to-5V	to-5V	NNS
cord-268139-tgpsu4qz	51	76	exonuclease	exonuclease	NN
cord-268139-tgpsu4qz	51	77	(	(	-LRB-
cord-268139-tgpsu4qz	51	78	Exo	Exo	NNP
cord-268139-tgpsu4qz	51	79	;	;	:
cord-268139-tgpsu4qz	51	80	nsp14	nsp14	ADD
cord-268139-tgpsu4qz	51	81	)	)	-RRB-
cord-268139-tgpsu4qz	51	82	,	,	,
cord-268139-tgpsu4qz	51	83	the	the	DT
cord-268139-tgpsu4qz	51	84	endoribonuclease	endoribonuclease	NN
cord-268139-tgpsu4qz	51	85	(	(	-LRB-
cord-268139-tgpsu4qz	51	86	Endo	Endo	NNP
cord-268139-tgpsu4qz	51	87	;	;	:
cord-268139-tgpsu4qz	51	88	nsp15	nsp15	ADD
cord-268139-tgpsu4qz	51	89	)	)	-RRB-
cord-268139-tgpsu4qz	51	90	,	,	,
cord-268139-tgpsu4qz	51	91	and	and	CC
cord-268139-tgpsu4qz	51	92	the	the	DT
cord-268139-tgpsu4qz	51	93	RNA	RNA	NNP
cord-268139-tgpsu4qz	51	94	methyltransferase	methyltransferase	NN
cord-268139-tgpsu4qz	51	95	(	(	-LRB-
cord-268139-tgpsu4qz	51	96	MT	MT	NNP
cord-268139-tgpsu4qz	51	97	;	;	:
cord-268139-tgpsu4qz	51	98	nsp16	nsp16	NNS
cord-268139-tgpsu4qz	51	99	)	)	-RRB-
cord-268139-tgpsu4qz	51	100	.	.	.
cord-268139-tgpsu4qz	52	1	(	(	-LRB-
cord-268139-tgpsu4qz	52	2	B	b	NN
cord-268139-tgpsu4qz	52	3	)	)	-RRB-
cord-268139-tgpsu4qz	53	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	53	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	53	3	proteins	protein	NNS
cord-268139-tgpsu4qz	53	4	.	.	.
cord-268139-tgpsu4qz	54	1	The	the	DT
cord-268139-tgpsu4qz	54	2	schematics	schematic	NNS
cord-268139-tgpsu4qz	54	3	illustrate	illustrate	VBP
cord-268139-tgpsu4qz	54	4	the	the	DT
cord-268139-tgpsu4qz	54	5	engineered	engineer	VBN
cord-268139-tgpsu4qz	54	6	deletions	deletion	NNS
cord-268139-tgpsu4qz	54	7	and	and	CC
cord-268139-tgpsu4qz	54	8	point	point	NN
cord-268139-tgpsu4qz	54	9	mutations	mutation	NNS
cord-268139-tgpsu4qz	54	10	within	within	IN
cord-268139-tgpsu4qz	54	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	54	12	.	.	.
cord-268139-tgpsu4qz	55	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	55	2	amino	amino	NN
cord-268139-tgpsu4qz	55	3	acid	acid	NN
cord-268139-tgpsu4qz	55	4	numbers	number	NNS
cord-268139-tgpsu4qz	55	5	are	be	VBP
cord-268139-tgpsu4qz	55	6	listed	list	VBN
cord-268139-tgpsu4qz	55	7	below	below	IN
cord-268139-tgpsu4qz	55	8	each	each	DT
cord-268139-tgpsu4qz	55	9	protein	protein	NN
cord-268139-tgpsu4qz	55	10	,	,	,
cord-268139-tgpsu4qz	55	11	and	and	CC
cord-268139-tgpsu4qz	55	12	the	the	DT
cord-268139-tgpsu4qz	55	13	predicted	predict	VBN
cord-268139-tgpsu4qz	55	14	protein	protein	NN
cord-268139-tgpsu4qz	55	15	size	size	NN
cord-268139-tgpsu4qz	55	16	(	(	-LRB-
cord-268139-tgpsu4qz	55	17	in	in	IN
cord-268139-tgpsu4qz	55	18	kilodaltons	kilodaltons	NNP
cord-268139-tgpsu4qz	55	19	)	)	-RRB-
cord-268139-tgpsu4qz	55	20	is	be	VBZ
cord-268139-tgpsu4qz	55	21	listed	list	VBN
cord-268139-tgpsu4qz	55	22	to	to	IN
cord-268139-tgpsu4qz	55	23	the	the	DT
cord-268139-tgpsu4qz	55	24	right	right	NN
cord-268139-tgpsu4qz	55	25	of	of	IN
cord-268139-tgpsu4qz	55	26	each	each	DT
cord-268139-tgpsu4qz	55	27	.	.	.
cord-268139-tgpsu4qz	56	1	The	the	DT
cord-268139-tgpsu4qz	56	2	amino	amino	NN
cord-268139-tgpsu4qz	56	3	-	-	HYPH
cord-268139-tgpsu4qz	56	4	terminal	terminal	NN
cord-268139-tgpsu4qz	56	5	charge	charge	NN
cord-268139-tgpsu4qz	56	6	-	-	HYPH
cord-268139-tgpsu4qz	56	7	to	to	IN
cord-268139-tgpsu4qz	56	8	-	-	HYPH
cord-268139-tgpsu4qz	56	9	alanine	alanine	NN
cord-268139-tgpsu4qz	56	10	mutations	mutation	NNS
cord-268139-tgpsu4qz	56	11	for	for	IN
cord-268139-tgpsu4qz	56	12	each	each	DT
cord-268139-tgpsu4qz	56	13	VUSB	VUSB	NNP
cord-268139-tgpsu4qz	56	14	mutants	mutant	NNS
cord-268139-tgpsu4qz	56	15	are	be	VBP
cord-268139-tgpsu4qz	56	16	listed	list	VBN
cord-268139-tgpsu4qz	56	17	below	below	IN
cord-268139-tgpsu4qz	56	18	the	the	DT
cord-268139-tgpsu4qz	56	19	bottom	bottom	NN
cord-268139-tgpsu4qz	56	20	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	56	21	protein	protein	NN
cord-268139-tgpsu4qz	56	22	.	.	.
cord-268139-tgpsu4qz	57	1	The	the	DT
cord-268139-tgpsu4qz	57	2	asterisks	asterisk	NNS
cord-268139-tgpsu4qz	57	3	(	(	-LRB-
cord-268139-tgpsu4qz	57	4	*	*	NFP
cord-268139-tgpsu4qz	57	5	)	)	-RRB-
cord-268139-tgpsu4qz	57	6	indicate	indicate	VBP
cord-268139-tgpsu4qz	57	7	mutants	mutant	NNS
cord-268139-tgpsu4qz	57	8	that	that	WDT
cord-268139-tgpsu4qz	57	9	did	do	VBD
cord-268139-tgpsu4qz	57	10	not	not	RB
cord-268139-tgpsu4qz	57	11	establish	establish	VB
cord-268139-tgpsu4qz	57	12	productive	productive	JJ
cord-268139-tgpsu4qz	57	13	infections	infection	NNS
cord-268139-tgpsu4qz	57	14	as	as	IN
cord-268139-tgpsu4qz	57	15	determined	determine	VBN
cord-268139-tgpsu4qz	57	16	by	by	IN
cord-268139-tgpsu4qz	57	17	lack	lack	NN
cord-268139-tgpsu4qz	57	18	of	of	IN
cord-268139-tgpsu4qz	57	19	recovered	recover	VBN
cord-268139-tgpsu4qz	57	20	virus	virus	NN
cord-268139-tgpsu4qz	57	21	from	from	IN
cord-268139-tgpsu4qz	57	22	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	57	23	cells	cell	NNS
cord-268139-tgpsu4qz	57	24	.	.	.
cord-268139-tgpsu4qz	58	1	lation	lation	NN
cord-268139-tgpsu4qz	58	2	of	of	IN
cord-268139-tgpsu4qz	58	3	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	58	4	within	within	IN
cord-268139-tgpsu4qz	58	5	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	58	6	(	(	-LRB-
cord-268139-tgpsu4qz	58	7	Baker	Baker	NNP
cord-268139-tgpsu4qz	58	8	et	et	NNP
cord-268139-tgpsu4qz	58	9	al	al	NNP
cord-268139-tgpsu4qz	58	10	.	.	NNP
cord-268139-tgpsu4qz	58	11	,	,	,
cord-268139-tgpsu4qz	58	12	1989	1989	CD
cord-268139-tgpsu4qz	58	13	(	(	-LRB-
cord-268139-tgpsu4qz	58	14	Baker	Baker	NNP
cord-268139-tgpsu4qz	58	15	et	et	NNP
cord-268139-tgpsu4qz	58	16	al	al	NNP
cord-268139-tgpsu4qz	58	17	.	.	.
cord-268139-tgpsu4qz	59	1	,	,	,
cord-268139-tgpsu4qz	59	2	,	,	,
cord-268139-tgpsu4qz	59	3	1993	1993	CD
cord-268139-tgpsu4qz	59	4	Denison	Denison	NNP
cord-268139-tgpsu4qz	59	5	and	and	CC
cord-268139-tgpsu4qz	59	6	Perlman	Perlman	NNP
cord-268139-tgpsu4qz	59	7	,	,	,
cord-268139-tgpsu4qz	59	8	1987	1987	CD
cord-268139-tgpsu4qz	59	9	;	;	:
cord-268139-tgpsu4qz	59	10	Denison	Denison	NNP
cord-268139-tgpsu4qz	59	11	and	and	CC
cord-268139-tgpsu4qz	59	12	Perlman	Perlman	NNP
cord-268139-tgpsu4qz	59	13	,	,	,
cord-268139-tgpsu4qz	59	14	1986	1986	CD
cord-268139-tgpsu4qz	59	15	;	;	:
cord-268139-tgpsu4qz	59	16	Denison	Denison	NNP
cord-268139-tgpsu4qz	59	17	et	et	NNP
cord-268139-tgpsu4qz	59	18	al	al	NNP
cord-268139-tgpsu4qz	59	19	.	.	.
cord-268139-tgpsu4qz	60	1	,	,	,
cord-268139-tgpsu4qz	60	2	1992	1992	CD
cord-268139-tgpsu4qz	60	3	Denison	Denison	NNP
cord-268139-tgpsu4qz	60	4	et	et	NNP
cord-268139-tgpsu4qz	60	5	al	al	NNP
cord-268139-tgpsu4qz	60	6	.	.	NNP
cord-268139-tgpsu4qz	60	7	,	,	,
cord-268139-tgpsu4qz	60	8	,	,	,
cord-268139-tgpsu4qz	60	9	1995	1995	CD
cord-268139-tgpsu4qz	60	10	.	.	.
cord-268139-tgpsu4qz	61	1	MHV	MHV	NNP
cord-268139-tgpsu4qz	61	2	mutants	mutant	NNS
cord-268139-tgpsu4qz	61	3	that	that	WDT
cord-268139-tgpsu4qz	61	4	are	be	VBP
cord-268139-tgpsu4qz	61	5	incapable	incapable	JJ
cord-268139-tgpsu4qz	61	6	of	of	IN
cord-268139-tgpsu4qz	61	7	liberating	liberate	VBG
cord-268139-tgpsu4qz	61	8	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	61	9	from	from	IN
cord-268139-tgpsu4qz	61	10	the	the	DT
cord-268139-tgpsu4qz	61	11	nascent	nascent	JJ
cord-268139-tgpsu4qz	61	12	polyprotein	polyprotein	NNP
cord-268139-tgpsu4qz	61	13	exhibit	exhibit	NN
cord-268139-tgpsu4qz	61	14	delayed	delay	VBN
cord-268139-tgpsu4qz	61	15	replication	replication	NN
cord-268139-tgpsu4qz	61	16	,	,	,
cord-268139-tgpsu4qz	61	17	diminished	diminish	VBN
cord-268139-tgpsu4qz	61	18	peak	peak	NN
cord-268139-tgpsu4qz	61	19	titers	titer	NNS
cord-268139-tgpsu4qz	61	20	,	,	,
cord-268139-tgpsu4qz	61	21	small	small	JJ
cord-268139-tgpsu4qz	61	22	plaques	plaque	NNS
cord-268139-tgpsu4qz	61	23	,	,	,
cord-268139-tgpsu4qz	61	24	and	and	CC
cord-268139-tgpsu4qz	61	25	reduced	reduce	VBN
cord-268139-tgpsu4qz	61	26	RNA	rna	NN
cord-268139-tgpsu4qz	61	27	synthesis	synthesis	NN
cord-268139-tgpsu4qz	61	28	compared	compare	VBN
cord-268139-tgpsu4qz	61	29	to	to	IN
cord-268139-tgpsu4qz	61	30	wild	wild	JJ
cord-268139-tgpsu4qz	61	31	-	-	HYPH
cord-268139-tgpsu4qz	61	32	type	type	NN
cord-268139-tgpsu4qz	61	33	controls	control	NNS
cord-268139-tgpsu4qz	61	34	.	.	.
cord-268139-tgpsu4qz	62	1	These	these	DT
cord-268139-tgpsu4qz	62	2	results	result	NNS
cord-268139-tgpsu4qz	62	3	emphasize	emphasize	VBP
cord-268139-tgpsu4qz	62	4	the	the	DT
cord-268139-tgpsu4qz	62	5	importance	importance	NN
cord-268139-tgpsu4qz	62	6	of	of	IN
cord-268139-tgpsu4qz	62	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	62	8	cleavage	cleavage	NN
cord-268139-tgpsu4qz	62	9	for	for	IN
cord-268139-tgpsu4qz	62	10	optimal	optimal	JJ
cord-268139-tgpsu4qz	62	11	viral	viral	JJ
cord-268139-tgpsu4qz	62	12	RNA	RNA	NNP
cord-268139-tgpsu4qz	62	13	synthesis	synthesis	NN
cord-268139-tgpsu4qz	62	14	and	and	CC
cord-268139-tgpsu4qz	62	15	suggest	suggest	VBP
cord-268139-tgpsu4qz	62	16	that	that	IN
cord-268139-tgpsu4qz	62	17	nsp1	nsp1	PRP
cord-268139-tgpsu4qz	62	18	might	may	MD
cord-268139-tgpsu4qz	62	19	play	play	VB
cord-268139-tgpsu4qz	62	20	an	an	DT
cord-268139-tgpsu4qz	62	21	important	important	JJ
cord-268139-tgpsu4qz	62	22	role	role	NN
cord-268139-tgpsu4qz	62	23	at	at	IN
cord-268139-tgpsu4qz	62	24	MHV	MHV	NNP
cord-268139-tgpsu4qz	62	25	replication	replication	NN
cord-268139-tgpsu4qz	62	26	complexes	complex	NNS
cord-268139-tgpsu4qz	62	27	.	.	.
cord-268139-tgpsu4qz	63	1	In	in	IN
cord-268139-tgpsu4qz	63	2	support	support	NN
cord-268139-tgpsu4qz	63	3	of	of	IN
cord-268139-tgpsu4qz	63	4	this	this	DT
cord-268139-tgpsu4qz	63	5	notion	notion	NN
cord-268139-tgpsu4qz	63	6	,	,	,
cord-268139-tgpsu4qz	63	7	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	63	8	localizes	localize	VBZ
cord-268139-tgpsu4qz	63	9	to	to	TO
cord-268139-tgpsu4qz	63	10	replication	replication	NN
cord-268139-tgpsu4qz	63	11	complexes	complex	NNS
cord-268139-tgpsu4qz	63	12	in	in	IN
cord-268139-tgpsu4qz	63	13	the	the	DT
cord-268139-tgpsu4qz	63	14	infected	infected	JJ
cord-268139-tgpsu4qz	63	15	cell	cell	NN
cord-268139-tgpsu4qz	63	16	cytoplasm	cytoplasm	NN
cord-268139-tgpsu4qz	63	17	during	during	IN
cord-268139-tgpsu4qz	63	18	times	time	NNS
cord-268139-tgpsu4qz	63	19	of	of	IN
cord-268139-tgpsu4qz	63	20	peak	peak	JJ
cord-268139-tgpsu4qz	63	21	viral	viral	JJ
cord-268139-tgpsu4qz	63	22	RNA	RNA	NNP
cord-268139-tgpsu4qz	63	23	synthesis	synthesis	NN
cord-268139-tgpsu4qz	63	24	,	,	,
cord-268139-tgpsu4qz	63	25	and	and	CC
cord-268139-tgpsu4qz	63	26	biochemical	biochemical	JJ
cord-268139-tgpsu4qz	63	27	experiments	experiment	NNS
cord-268139-tgpsu4qz	63	28	demonstrate	demonstrate	VBP
cord-268139-tgpsu4qz	63	29	interactions	interaction	NNS
cord-268139-tgpsu4qz	63	30	between	between	IN
cord-268139-tgpsu4qz	63	31	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	63	32	and	and	CC
cord-268139-tgpsu4qz	63	33	two	two	CD
cord-268139-tgpsu4qz	63	34	other	other	JJ
cord-268139-tgpsu4qz	63	35	replication	replication	NN
cord-268139-tgpsu4qz	63	36	complexassociated	complexassociate	VBN
cord-268139-tgpsu4qz	63	37	proteins	protein	NNS
cord-268139-tgpsu4qz	63	38	(	(	-LRB-
cord-268139-tgpsu4qz	63	39	nsp7	nsp7	JJ
cord-268139-tgpsu4qz	63	40	and	and	CC
cord-268139-tgpsu4qz	63	41	nsp10	nsp10	ADD
cord-268139-tgpsu4qz	63	42	)	)	-RRB-
cord-268139-tgpsu4qz	63	43	.	.	.
cord-268139-tgpsu4qz	64	1	However	however	RB
cord-268139-tgpsu4qz	64	2	,	,	,
cord-268139-tgpsu4qz	64	3	later	later	RB
cord-268139-tgpsu4qz	64	4	in	in	IN
cord-268139-tgpsu4qz	64	5	infection	infection	NN
cord-268139-tgpsu4qz	64	6	,	,	,
cord-268139-tgpsu4qz	64	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	64	8	is	be	VBZ
cord-268139-tgpsu4qz	64	9	distinct	distinct	JJ
cord-268139-tgpsu4qz	64	10	from	from	IN
cord-268139-tgpsu4qz	64	11	replication	replication	NN
cord-268139-tgpsu4qz	64	12	complexes	complex	NNS
cord-268139-tgpsu4qz	64	13	and	and	CC
cord-268139-tgpsu4qz	64	14	instead	instead	RB
cord-268139-tgpsu4qz	64	15	co	co	RB
cord-268139-tgpsu4qz	64	16	-	-	VBZ
cord-268139-tgpsu4qz	64	17	localizes	localize	VBZ
cord-268139-tgpsu4qz	64	18	with	with	IN
cord-268139-tgpsu4qz	64	19	MHV	MHV	NNP
cord-268139-tgpsu4qz	64	20	structural	structural	JJ
cord-268139-tgpsu4qz	64	21	proteins	protein	NNS
cord-268139-tgpsu4qz	64	22	at	at	IN
cord-268139-tgpsu4qz	64	23	virion	virion	NN
cord-268139-tgpsu4qz	64	24	assembly	assembly	NN
cord-268139-tgpsu4qz	64	25	sites	site	NNS
cord-268139-tgpsu4qz	64	26	.	.	.
cord-268139-tgpsu4qz	65	1	It	-PRON-	PRP
cord-268139-tgpsu4qz	65	2	has	have	VBZ
cord-268139-tgpsu4qz	65	3	also	also	RB
cord-268139-tgpsu4qz	65	4	been	be	VBN
cord-268139-tgpsu4qz	65	5	reported	report	VBN
cord-268139-tgpsu4qz	65	6	that	that	IN
cord-268139-tgpsu4qz	65	7	exogenous	exogenous	JJ
cord-268139-tgpsu4qz	65	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	65	9	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	65	10	expression	expression	NN
cord-268139-tgpsu4qz	65	11	induces	induce	VBZ
cord-268139-tgpsu4qz	65	12	cell	cell	NN
cord-268139-tgpsu4qz	65	13	-	-	HYPH
cord-268139-tgpsu4qz	65	14	cycle	cycle	NN
cord-268139-tgpsu4qz	65	15	arrest	arrest	NN
cord-268139-tgpsu4qz	65	16	(	(	-LRB-
cord-268139-tgpsu4qz	65	17	Chen	Chen	NNP
cord-268139-tgpsu4qz	65	18	et	et	NNP
cord-268139-tgpsu4qz	65	19	al	al	NNP
cord-268139-tgpsu4qz	65	20	.	.	NNP
cord-268139-tgpsu4qz	65	21	,	,	,
cord-268139-tgpsu4qz	65	22	2004	2004	CD
cord-268139-tgpsu4qz	65	23	)	)	-RRB-
cord-268139-tgpsu4qz	65	24	.	.	.
cord-268139-tgpsu4qz	66	1	Together	together	RB
cord-268139-tgpsu4qz	66	2	,	,	,
cord-268139-tgpsu4qz	66	3	these	these	DT
cord-268139-tgpsu4qz	66	4	results	result	NNS
cord-268139-tgpsu4qz	66	5	led	lead	VBD
cord-268139-tgpsu4qz	66	6	to	to	IN
cord-268139-tgpsu4qz	66	7	the	the	DT
cord-268139-tgpsu4qz	66	8	hypothesis	hypothesis	NN
cord-268139-tgpsu4qz	66	9	that	that	IN
cord-268139-tgpsu4qz	66	10	nsp1	nsp1	PRP
cord-268139-tgpsu4qz	66	11	may	may	MD
cord-268139-tgpsu4qz	66	12	participate	participate	VB
cord-268139-tgpsu4qz	66	13	in	in	IN
cord-268139-tgpsu4qz	66	14	multiple	multiple	JJ
cord-268139-tgpsu4qz	66	15	stages	stage	NNS
cord-268139-tgpsu4qz	66	16	of	of	IN
cord-268139-tgpsu4qz	66	17	the	the	DT
cord-268139-tgpsu4qz	66	18	MHV	MHV	NNP
cord-268139-tgpsu4qz	66	19	life	life	NN
cord-268139-tgpsu4qz	66	20	cycle	cycle	NN
cord-268139-tgpsu4qz	66	21	.	.	.
cord-268139-tgpsu4qz	67	1	Still	still	RB
cord-268139-tgpsu4qz	67	2	,	,	,
cord-268139-tgpsu4qz	67	3	the	the	DT
cord-268139-tgpsu4qz	67	4	function	function	NN
cord-268139-tgpsu4qz	67	5	of	of	IN
cord-268139-tgpsu4qz	67	6	this	this	DT
cord-268139-tgpsu4qz	67	7	protein	protein	NN
cord-268139-tgpsu4qz	67	8	remains	remain	VBZ
cord-268139-tgpsu4qz	67	9	a	a	DT
cord-268139-tgpsu4qz	67	10	mystery	mystery	NN
cord-268139-tgpsu4qz	67	11	as	as	IN
cord-268139-tgpsu4qz	67	12	analysis	analysis	NN
cord-268139-tgpsu4qz	67	13	of	of	IN
cord-268139-tgpsu4qz	67	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	67	15	primary	primary	JJ
cord-268139-tgpsu4qz	67	16	amino	amino	NN
cord-268139-tgpsu4qz	67	17	acid	acid	NN
cord-268139-tgpsu4qz	67	18	sequence	sequence	NN
cord-268139-tgpsu4qz	67	19	does	do	VBZ
cord-268139-tgpsu4qz	67	20	not	not	RB
cord-268139-tgpsu4qz	67	21	reveal	reveal	VB
cord-268139-tgpsu4qz	67	22	common	common	JJ
cord-268139-tgpsu4qz	67	23	motifs	motif	NNS
cord-268139-tgpsu4qz	67	24	or	or	CC
cord-268139-tgpsu4qz	67	25	protein	protein	NN
cord-268139-tgpsu4qz	67	26	homologs	homolog	NNS
cord-268139-tgpsu4qz	67	27	,	,	,
cord-268139-tgpsu4qz	67	28	and	and	CC
cord-268139-tgpsu4qz	67	29	the	the	DT
cord-268139-tgpsu4qz	67	30	nsp1	nsp1	NNP
cord-268139-tgpsu4qz	67	31	crystal	crystal	NN
cord-268139-tgpsu4qz	67	32	structure	structure	NN
cord-268139-tgpsu4qz	67	33	has	have	VBZ
cord-268139-tgpsu4qz	67	34	not	not	RB
cord-268139-tgpsu4qz	67	35	been	be	VBN
cord-268139-tgpsu4qz	67	36	solved	solve	VBN
cord-268139-tgpsu4qz	67	37	.	.	.
cord-268139-tgpsu4qz	68	1	The	the	DT
cord-268139-tgpsu4qz	68	2	goal	goal	NN
cord-268139-tgpsu4qz	68	3	of	of	IN
cord-268139-tgpsu4qz	68	4	the	the	DT
cord-268139-tgpsu4qz	68	5	current	current	JJ
cord-268139-tgpsu4qz	68	6	study	study	NN
cord-268139-tgpsu4qz	68	7	was	be	VBD
cord-268139-tgpsu4qz	68	8	to	to	TO
cord-268139-tgpsu4qz	68	9	determine	determine	VB
cord-268139-tgpsu4qz	68	10	the	the	DT
cord-268139-tgpsu4qz	68	11	role	role	NN
cord-268139-tgpsu4qz	68	12	of	of	IN
cord-268139-tgpsu4qz	68	13	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	68	14	during	during	IN
cord-268139-tgpsu4qz	68	15	MHV	MHV	NNP
cord-268139-tgpsu4qz	68	16	replication	replication	NN
cord-268139-tgpsu4qz	68	17	in	in	IN
cord-268139-tgpsu4qz	68	18	cell	cell	NN
cord-268139-tgpsu4qz	68	19	culture	culture	NN
cord-268139-tgpsu4qz	68	20	and	and	CC
cord-268139-tgpsu4qz	68	21	to	to	TO
cord-268139-tgpsu4qz	68	22	identify	identify	VB
cord-268139-tgpsu4qz	68	23	regions	region	NNS
cord-268139-tgpsu4qz	68	24	of	of	IN
cord-268139-tgpsu4qz	68	25	the	the	DT
cord-268139-tgpsu4qz	68	26	protein	protein	NN
cord-268139-tgpsu4qz	68	27	that	that	WDT
cord-268139-tgpsu4qz	68	28	are	be	VBP
cord-268139-tgpsu4qz	68	29	critical	critical	JJ
cord-268139-tgpsu4qz	68	30	for	for	IN
cord-268139-tgpsu4qz	68	31	its	-PRON-	PRP$
cord-268139-tgpsu4qz	68	32	function	function	NN
cord-268139-tgpsu4qz	68	33	.	.	.
cord-268139-tgpsu4qz	69	1	Using	use	VBG
cord-268139-tgpsu4qz	69	2	the	the	DT
cord-268139-tgpsu4qz	69	3	reverse	reverse	JJ
cord-268139-tgpsu4qz	69	4	genetic	genetic	JJ
cord-268139-tgpsu4qz	69	5	system	system	NN
cord-268139-tgpsu4qz	69	6	for	for	IN
cord-268139-tgpsu4qz	69	7	MHV	MHV	NNP
cord-268139-tgpsu4qz	69	8	developed	develop	VBN
cord-268139-tgpsu4qz	69	9	by	by	IN
cord-268139-tgpsu4qz	69	10	Yount	Yount	NNP
cord-268139-tgpsu4qz	69	11	et	et	NNP
cord-268139-tgpsu4qz	69	12	al	al	NNP
cord-268139-tgpsu4qz	69	13	.	.	.
cord-268139-tgpsu4qz	70	1	(	(	-LRB-
cord-268139-tgpsu4qz	70	2	2002	2002	CD
cord-268139-tgpsu4qz	70	3	)	)	-RRB-
cord-268139-tgpsu4qz	70	4	,	,	,
cord-268139-tgpsu4qz	70	5	genomic	genomic	JJ
cord-268139-tgpsu4qz	70	6	RNA	RNA	NNP
cord-268139-tgpsu4qz	70	7	molecules	molecule	NNS
cord-268139-tgpsu4qz	70	8	that	that	WDT
cord-268139-tgpsu4qz	70	9	contained	contain	VBD
cord-268139-tgpsu4qz	70	10	deletions	deletion	NNS
cord-268139-tgpsu4qz	70	11	or	or	CC
cord-268139-tgpsu4qz	70	12	point	point	NN
cord-268139-tgpsu4qz	70	13	mutations	mutation	NNS
cord-268139-tgpsu4qz	70	14	within	within	IN
cord-268139-tgpsu4qz	70	15	the	the	DT
cord-268139-tgpsu4qz	70	16	nsp1-coding	nsp1-coding	NNP
cord-268139-tgpsu4qz	70	17	region	region	NN
cord-268139-tgpsu4qz	70	18	were	be	VBD
cord-268139-tgpsu4qz	70	19	generated	generate	VBN
cord-268139-tgpsu4qz	70	20	and	and	CC
cord-268139-tgpsu4qz	70	21	tested	test	VBN
cord-268139-tgpsu4qz	70	22	for	for	IN
cord-268139-tgpsu4qz	70	23	their	-PRON-	PRP$
cord-268139-tgpsu4qz	70	24	capacity	capacity	NN
cord-268139-tgpsu4qz	70	25	to	to	TO
cord-268139-tgpsu4qz	70	26	produce	produce	VB
cord-268139-tgpsu4qz	70	27	infectious	infectious	JJ
cord-268139-tgpsu4qz	70	28	virus	virus	NN
cord-268139-tgpsu4qz	70	29	following	follow	VBG
cord-268139-tgpsu4qz	70	30	transfection	transfection	NN
cord-268139-tgpsu4qz	70	31	into	into	IN
cord-268139-tgpsu4qz	70	32	permissive	permissive	JJ
cord-268139-tgpsu4qz	70	33	cells	cell	NNS
cord-268139-tgpsu4qz	70	34	.	.	.
cord-268139-tgpsu4qz	71	1	The	the	DT
cord-268139-tgpsu4qz	71	2	results	result	NNS
cord-268139-tgpsu4qz	71	3	demonstrate	demonstrate	VBP
cord-268139-tgpsu4qz	71	4	that	that	IN
cord-268139-tgpsu4qz	71	5	the	the	DT
cord-268139-tgpsu4qz	71	6	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	71	7	-	-	HYPH
cord-268139-tgpsu4qz	71	8	terminal	terminal	NN
cord-268139-tgpsu4qz	71	9	half	half	NN
cord-268139-tgpsu4qz	71	10	of	of	IN
cord-268139-tgpsu4qz	71	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	71	12	is	be	VBZ
cord-268139-tgpsu4qz	71	13	dispensable	dispensable	JJ
cord-268139-tgpsu4qz	71	14	for	for	IN
cord-268139-tgpsu4qz	71	15	replication	replication	NN
cord-268139-tgpsu4qz	71	16	in	in	IN
cord-268139-tgpsu4qz	71	17	culture	culture	NN
cord-268139-tgpsu4qz	71	18	but	but	CC
cord-268139-tgpsu4qz	71	19	is	be	VBZ
cord-268139-tgpsu4qz	71	20	important	important	JJ
cord-268139-tgpsu4qz	71	21	for	for	IN
cord-268139-tgpsu4qz	71	22	efficient	efficient	JJ
cord-268139-tgpsu4qz	71	23	proteolytic	proteolytic	JJ
cord-268139-tgpsu4qz	71	24	cleavage	cleavage	NN
cord-268139-tgpsu4qz	71	25	of	of	IN
cord-268139-tgpsu4qz	71	26	the	the	DT
cord-268139-tgpsu4qz	71	27	protein	protein	NN
cord-268139-tgpsu4qz	71	28	and	and	CC
cord-268139-tgpsu4qz	71	29	optimal	optimal	JJ
cord-268139-tgpsu4qz	71	30	viral	viral	JJ
cord-268139-tgpsu4qz	71	31	replication	replication	NN
cord-268139-tgpsu4qz	71	32	.	.	.
cord-268139-tgpsu4qz	72	1	In	in	IN
cord-268139-tgpsu4qz	72	2	contrast	contrast	NN
cord-268139-tgpsu4qz	72	3	,	,	,
cord-268139-tgpsu4qz	72	4	deletion	deletion	NN
cord-268139-tgpsu4qz	72	5	of	of	IN
cord-268139-tgpsu4qz	72	6	the	the	DT
cord-268139-tgpsu4qz	72	7	entire	entire	JJ
cord-268139-tgpsu4qz	72	8	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	72	9	protein	protein	NN
cord-268139-tgpsu4qz	72	10	or	or	CC
cord-268139-tgpsu4qz	72	11	a	a	DT
cord-268139-tgpsu4qz	72	12	middle	middle	JJ
cord-268139-tgpsu4qz	72	13	region	region	NN
cord-268139-tgpsu4qz	72	14	of	of	IN
cord-268139-tgpsu4qz	72	15	the	the	DT
cord-268139-tgpsu4qz	72	16	protein	protein	NN
cord-268139-tgpsu4qz	72	17	resulted	result	VBD
cord-268139-tgpsu4qz	72	18	in	in	IN
cord-268139-tgpsu4qz	72	19	the	the	DT
cord-268139-tgpsu4qz	72	20	lack	lack	NN
cord-268139-tgpsu4qz	72	21	of	of	IN
cord-268139-tgpsu4qz	72	22	virus	virus	NN
cord-268139-tgpsu4qz	72	23	recovered	recover	VBN
cord-268139-tgpsu4qz	72	24	from	from	IN
cord-268139-tgpsu4qz	72	25	electroporated	electroporated	JJ
cord-268139-tgpsu4qz	72	26	cells	cell	NNS
cord-268139-tgpsu4qz	72	27	.	.	.
cord-268139-tgpsu4qz	73	1	Analysis	analysis	NN
cord-268139-tgpsu4qz	73	2	of	of	IN
cord-268139-tgpsu4qz	73	3	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	73	4	point	point	NN
cord-268139-tgpsu4qz	73	5	mutants	mutant	NNS
cord-268139-tgpsu4qz	73	6	identified	identify	VBD
cord-268139-tgpsu4qz	73	7	residues	residue	NNS
cord-268139-tgpsu4qz	73	8	important	important	JJ
cord-268139-tgpsu4qz	73	9	for	for	IN
cord-268139-tgpsu4qz	73	10	viral	viral	JJ
cord-268139-tgpsu4qz	73	11	replication	replication	NN
cord-268139-tgpsu4qz	73	12	and	and	CC
cord-268139-tgpsu4qz	73	13	RNA	RNA	NNP
cord-268139-tgpsu4qz	73	14	synthesis	synthesis	NN
cord-268139-tgpsu4qz	73	15	.	.	.
cord-268139-tgpsu4qz	74	1	The	the	DT
cord-268139-tgpsu4qz	74	2	results	result	NNS
cord-268139-tgpsu4qz	74	3	of	of	IN
cord-268139-tgpsu4qz	74	4	this	this	DT
cord-268139-tgpsu4qz	74	5	study	study	NN
cord-268139-tgpsu4qz	74	6	provide	provide	VB
cord-268139-tgpsu4qz	74	7	valuable	valuable	JJ
cord-268139-tgpsu4qz	74	8	information	information	NN
cord-268139-tgpsu4qz	74	9	regarding	regard	VBG
cord-268139-tgpsu4qz	74	10	the	the	DT
cord-268139-tgpsu4qz	74	11	possible	possible	JJ
cord-268139-tgpsu4qz	74	12	functions	function	NNS
cord-268139-tgpsu4qz	74	13	of	of	IN
cord-268139-tgpsu4qz	74	14	the	the	DT
cord-268139-tgpsu4qz	74	15	amino	amino	NN
cord-268139-tgpsu4qz	74	16	-	-	HYPH
cord-268139-tgpsu4qz	74	17	terminal	terminal	NN
cord-268139-tgpsu4qz	74	18	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	74	19	protein	protein	NN
cord-268139-tgpsu4qz	74	20	in	in	IN
cord-268139-tgpsu4qz	74	21	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	74	22	replication	replication	NN
cord-268139-tgpsu4qz	74	23	.	.	.
cord-268139-tgpsu4qz	75	1	The	the	DT
cord-268139-tgpsu4qz	75	2	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	75	3	-	-	HYPH
cord-268139-tgpsu4qz	75	4	terminal	terminal	NN
cord-268139-tgpsu4qz	75	5	half	half	NN
cord-268139-tgpsu4qz	75	6	of	of	IN
cord-268139-tgpsu4qz	75	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	75	8	is	be	VBZ
cord-268139-tgpsu4qz	75	9	dispensable	dispensable	JJ
cord-268139-tgpsu4qz	75	10	for	for	IN
cord-268139-tgpsu4qz	75	11	MHV	MHV	NNP
cord-268139-tgpsu4qz	75	12	replication	replication	NN
cord-268139-tgpsu4qz	75	13	To	to	TO
cord-268139-tgpsu4qz	75	14	determine	determine	VB
cord-268139-tgpsu4qz	75	15	whether	whether	IN
cord-268139-tgpsu4qz	75	16	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	75	17	is	be	VBZ
cord-268139-tgpsu4qz	75	18	required	require	VBN
cord-268139-tgpsu4qz	75	19	for	for	IN
cord-268139-tgpsu4qz	75	20	MHV	MHV	NNP
cord-268139-tgpsu4qz	75	21	replication	replication	NN
cord-268139-tgpsu4qz	75	22	,	,	,
cord-268139-tgpsu4qz	75	23	viruses	virus	NNS
cord-268139-tgpsu4qz	75	24	were	be	VBD
cord-268139-tgpsu4qz	75	25	engineered	engineer	VBN
cord-268139-tgpsu4qz	75	26	to	to	TO
cord-268139-tgpsu4qz	75	27	lack	lack	VB
cord-268139-tgpsu4qz	75	28	nsp1	nsp1	CD
cord-268139-tgpsu4qz	75	29	residues	residue	NNS
cord-268139-tgpsu4qz	76	1	A	a	DT
cord-268139-tgpsu4qz	76	2	2	2	CD
cord-268139-tgpsu4qz	76	3	through	through	IN
cord-268139-tgpsu4qz	76	4	L	l	NN
cord-268139-tgpsu4qz	76	5	241	241	CD
cord-268139-tgpsu4qz	77	1	(	(	-LRB-
cord-268139-tgpsu4qz	77	2	nsp1DFL	nsp1DFL	NNP
cord-268139-tgpsu4qz	77	3	)	)	-RRB-
cord-268139-tgpsu4qz	77	4	or	or	CC
cord-268139-tgpsu4qz	78	1	to	to	TO
cord-268139-tgpsu4qz	78	2	express	express	VB
cord-268139-tgpsu4qz	78	3	truncated	truncated	JJ
cord-268139-tgpsu4qz	78	4	forms	form	NNS
cord-268139-tgpsu4qz	78	5	of	of	IN
cord-268139-tgpsu4qz	78	6	the	the	DT
cord-268139-tgpsu4qz	78	7	protein	protein	NN
cord-268139-tgpsu4qz	78	8	lacking	lack	VBG
cord-268139-tgpsu4qz	78	9	either	either	CC
cord-268139-tgpsu4qz	78	10	Q	Q	NNP
cord-268139-tgpsu4qz	78	11	87	87	CD
cord-268139-tgpsu4qz	78	12	through	through	IN
cord-268139-tgpsu4qz	78	13	N	n	CD
cord-268139-tgpsu4qz	78	14	164	164	CD
cord-268139-tgpsu4qz	78	15	(	(	-LRB-
cord-268139-tgpsu4qz	78	16	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	78	17	)	)	-RRB-
cord-268139-tgpsu4qz	78	18	or	or	CC
cord-268139-tgpsu4qz	78	19	K	K	NNP
cord-268139-tgpsu4qz	78	20	124	124	CD
cord-268139-tgpsu4qz	78	21	through	through	IN
cord-268139-tgpsu4qz	78	22	L	L	NNP
cord-268139-tgpsu4qz	78	23	241	241	CD
cord-268139-tgpsu4qz	79	1	(	(	-LRB-
cord-268139-tgpsu4qz	79	2	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	79	3	)	)	-RRB-
cord-268139-tgpsu4qz	80	1	(	(	-LRB-
cord-268139-tgpsu4qz	80	2	Fig	Fig	NNP
cord-268139-tgpsu4qz	80	3	.	.	.
cord-268139-tgpsu4qz	80	4	1B	1b	CD
cord-268139-tgpsu4qz	80	5	)	)	-RRB-
cord-268139-tgpsu4qz	80	6	.	.	.
cord-268139-tgpsu4qz	81	1	All	all	DT
cord-268139-tgpsu4qz	81	2	deletion	deletion	NN
cord-268139-tgpsu4qz	81	3	constructs	construct	NNS
cord-268139-tgpsu4qz	81	4	were	be	VBD
cord-268139-tgpsu4qz	81	5	designed	design	VBN
cord-268139-tgpsu4qz	81	6	to	to	TO
cord-268139-tgpsu4qz	81	7	maintain	maintain	VB
cord-268139-tgpsu4qz	81	8	the	the	DT
cord-268139-tgpsu4qz	81	9	start	start	NN
cord-268139-tgpsu4qz	81	10	codon	codon	NN
cord-268139-tgpsu4qz	81	11	,	,	,
cord-268139-tgpsu4qz	81	12	the	the	DT
cord-268139-tgpsu4qz	81	13	translational	translational	JJ
cord-268139-tgpsu4qz	81	14	reading	reading	NN
cord-268139-tgpsu4qz	81	15	frame	frame	NN
cord-268139-tgpsu4qz	81	16	,	,	,
cord-268139-tgpsu4qz	81	17	and	and	CC
cord-268139-tgpsu4qz	81	18	the	the	DT
cord-268139-tgpsu4qz	81	19	minimal	minimal	JJ
cord-268139-tgpsu4qz	81	20	residues	residue	NNS
cord-268139-tgpsu4qz	81	21	thought	think	VBN
cord-268139-tgpsu4qz	81	22	to	to	TO
cord-268139-tgpsu4qz	81	23	be	be	VB
cord-268139-tgpsu4qz	81	24	required	require	VBN
cord-268139-tgpsu4qz	81	25	for	for	IN
cord-268139-tgpsu4qz	81	26	cleavage	cleavage	NN
cord-268139-tgpsu4qz	81	27	of	of	IN
cord-268139-tgpsu4qz	81	28	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	81	29	from	from	IN
cord-268139-tgpsu4qz	81	30	the	the	DT
cord-268139-tgpsu4qz	81	31	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	81	32	by	by	IN
cord-268139-tgpsu4qz	81	33	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	82	1	(	(	-LRB-
cord-268139-tgpsu4qz	82	2	nsp1	nsp1	NN
cord-268139-tgpsu4qz	82	3	residues	residue	VBZ
cord-268139-tgpsu4qz	82	4	K	K	NNP
cord-268139-tgpsu4qz	82	5	243	243	CD
cord-268139-tgpsu4qz	82	6	through	through	IN
cord-268139-tgpsu4qz	82	7	G	G	NNP
cord-268139-tgpsu4qz	82	8	247	247	CD
cord-268139-tgpsu4qz	82	9	)	)	-RRB-
cord-268139-tgpsu4qz	82	10	.	.	.
cord-268139-tgpsu4qz	83	1	Mutations	mutation	NNS
cord-268139-tgpsu4qz	83	2	were	be	VBD
cord-268139-tgpsu4qz	83	3	engineered	engineer	VBN
cord-268139-tgpsu4qz	83	4	into	into	IN
cord-268139-tgpsu4qz	83	5	the	the	DT
cord-268139-tgpsu4qz	83	6	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	83	7	region	region	NN
cord-268139-tgpsu4qz	83	8	within	within	IN
cord-268139-tgpsu4qz	83	9	the	the	DT
cord-268139-tgpsu4qz	83	10	MHV	MHV	NNP
cord-268139-tgpsu4qz	83	11	infectious	infectious	JJ
cord-268139-tgpsu4qz	83	12	cDNA	cdna	NN
cord-268139-tgpsu4qz	83	13	(	(	-LRB-
cord-268139-tgpsu4qz	83	14	icMHV	icmhv	NN
cord-268139-tgpsu4qz	83	15	)	)	-RRB-
cord-268139-tgpsu4qz	83	16	fragment	fragment	VBP
cord-268139-tgpsu4qz	83	17	A	a	DT
cord-268139-tgpsu4qz	83	18	plasmid	plasmid	NN
cord-268139-tgpsu4qz	83	19	.	.	.
cord-268139-tgpsu4qz	84	1	The	the	DT
cord-268139-tgpsu4qz	84	2	mutagenized	mutagenized	JJ
cord-268139-tgpsu4qz	84	3	plasmids	plasmid	NNS
cord-268139-tgpsu4qz	84	4	were	be	VBD
cord-268139-tgpsu4qz	84	5	then	then	RB
cord-268139-tgpsu4qz	84	6	used	use	VBN
cord-268139-tgpsu4qz	84	7	to	to	TO
cord-268139-tgpsu4qz	84	8	assemble	assemble	VB
cord-268139-tgpsu4qz	84	9	full	full	JJ
cord-268139-tgpsu4qz	84	10	-	-	HYPH
cord-268139-tgpsu4qz	84	11	length	length	NN
cord-268139-tgpsu4qz	84	12	MHV	MHV	NNP
cord-268139-tgpsu4qz	84	13	cDNA	cdna	NN
cord-268139-tgpsu4qz	84	14	,	,	,
cord-268139-tgpsu4qz	84	15	which	which	WDT
cord-268139-tgpsu4qz	84	16	was	be	VBD
cord-268139-tgpsu4qz	84	17	transcribed	transcribe	VBN
cord-268139-tgpsu4qz	84	18	in	in	IN
cord-268139-tgpsu4qz	84	19	vitro	vitro	FW
cord-268139-tgpsu4qz	84	20	to	to	TO
cord-268139-tgpsu4qz	84	21	yield	yield	VB
cord-268139-tgpsu4qz	84	22	MHV	MHV	NNP
cord-268139-tgpsu4qz	84	23	genomic	genomic	JJ
cord-268139-tgpsu4qz	84	24	RNA	RNA	NNP
cord-268139-tgpsu4qz	84	25	(	(	-LRB-
cord-268139-tgpsu4qz	84	26	Yount	Yount	NNP
cord-268139-tgpsu4qz	84	27	et	et	NNP
cord-268139-tgpsu4qz	84	28	al	al	NNP
cord-268139-tgpsu4qz	84	29	.	.	NNP
cord-268139-tgpsu4qz	84	30	,	,	,
cord-268139-tgpsu4qz	84	31	2002	2002	CD
cord-268139-tgpsu4qz	84	32	)	)	-RRB-
cord-268139-tgpsu4qz	84	33	.	.	.
cord-268139-tgpsu4qz	85	1	BHK	BHK	NNP
cord-268139-tgpsu4qz	85	2	-	-	HYPH
cord-268139-tgpsu4qz	85	3	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	85	4	cells	cell	NNS
cord-268139-tgpsu4qz	85	5	were	be	VBD
cord-268139-tgpsu4qz	85	6	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	85	7	with	with	IN
cord-268139-tgpsu4qz	85	8	either	either	CC
cord-268139-tgpsu4qz	85	9	assembled	assemble	VBN
cord-268139-tgpsu4qz	85	10	wild	wild	JJ
cord-268139-tgpsu4qz	85	11	-	-	HYPH
cord-268139-tgpsu4qz	85	12	type	type	NN
cord-268139-tgpsu4qz	85	13	(	(	-LRB-
cord-268139-tgpsu4qz	85	14	icwt	icwt	NNP
cord-268139-tgpsu4qz	85	15	)	)	-RRB-
cord-268139-tgpsu4qz	85	16	or	or	CC
cord-268139-tgpsu4qz	85	17	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	85	18	mutant	mutant	JJ
cord-268139-tgpsu4qz	85	19	genomic	genomic	JJ
cord-268139-tgpsu4qz	85	20	RNA	RNA	NNP
cord-268139-tgpsu4qz	85	21	transcripts	transcript	NNS
cord-268139-tgpsu4qz	85	22	.	.	.
cord-268139-tgpsu4qz	86	1	Cells	cell	NNS
cord-268139-tgpsu4qz	86	2	were	be	VBD
cord-268139-tgpsu4qz	86	3	incubated	incubate	VBN
cord-268139-tgpsu4qz	86	4	at	at	IN
cord-268139-tgpsu4qz	86	5	37	37	CD
cord-268139-tgpsu4qz	86	6	-C	-C	NNS
cord-268139-tgpsu4qz	86	7	and	and	CC
cord-268139-tgpsu4qz	86	8	were	be	VBD
cord-268139-tgpsu4qz	86	9	monitored	monitor	VBN
cord-268139-tgpsu4qz	86	10	for	for	IN
cord-268139-tgpsu4qz	86	11	syncytia	syncytia	NN
cord-268139-tgpsu4qz	86	12	formation	formation	NN
cord-268139-tgpsu4qz	86	13	,	,	,
cord-268139-tgpsu4qz	86	14	a	a	DT
cord-268139-tgpsu4qz	86	15	cytopathic	cytopathic	JJ
cord-268139-tgpsu4qz	86	16	effect	effect	NN
cord-268139-tgpsu4qz	86	17	(	(	-LRB-
cord-268139-tgpsu4qz	86	18	CPE	CPE	NNP
cord-268139-tgpsu4qz	86	19	)	)	-RRB-
cord-268139-tgpsu4qz	86	20	of	of	IN
cord-268139-tgpsu4qz	86	21	MHV	MHV	NNP
cord-268139-tgpsu4qz	86	22	replication	replication	NN
cord-268139-tgpsu4qz	86	23	.	.	.
cord-268139-tgpsu4qz	87	1	Virus	virus	NN
cord-268139-tgpsu4qz	87	2	-	-	HYPH
cord-268139-tgpsu4qz	87	3	induced	induce	VBN
cord-268139-tgpsu4qz	87	4	syncytia	syncytia	NN
cord-268139-tgpsu4qz	87	5	were	be	VBD
cord-268139-tgpsu4qz	87	6	detected	detect	VBN
cord-268139-tgpsu4qz	87	7	at	at	IN
cord-268139-tgpsu4qz	87	8	24	24	CD
cord-268139-tgpsu4qz	87	9	h	h	NN
cord-268139-tgpsu4qz	87	10	postelectroporation	postelectroporation	NN
cord-268139-tgpsu4qz	87	11	(	(	-LRB-
cord-268139-tgpsu4qz	87	12	p.e	p.e	NNP
cord-268139-tgpsu4qz	87	13	.	.	NNP
cord-268139-tgpsu4qz	87	14	)	)	-RRB-
cord-268139-tgpsu4qz	87	15	in	in	IN
cord-268139-tgpsu4qz	87	16	cells	cell	NNS
cord-268139-tgpsu4qz	87	17	transfected	transfecte	VBN
cord-268139-tgpsu4qz	87	18	with	with	IN
cord-268139-tgpsu4qz	87	19	icwt	icwt	NNP
cord-268139-tgpsu4qz	87	20	genomic	genomic	NNP
cord-268139-tgpsu4qz	87	21	RNA	RNA	NNP
cord-268139-tgpsu4qz	87	22	and	and	CC
cord-268139-tgpsu4qz	87	23	at	at	IN
cord-268139-tgpsu4qz	87	24	72	72	CD
cord-268139-tgpsu4qz	87	25	h	h	NN
cord-268139-tgpsu4qz	87	26	p.e	p.e	NNP
cord-268139-tgpsu4qz	87	27	.	.	.
cord-268139-tgpsu4qz	88	1	in	in	IN
cord-268139-tgpsu4qz	88	2	cells	cell	NNS
cord-268139-tgpsu4qz	88	3	transfected	transfecte	VBN
cord-268139-tgpsu4qz	88	4	with	with	IN
cord-268139-tgpsu4qz	88	5	genomic	genomic	JJ
cord-268139-tgpsu4qz	88	6	RNA	RNA	NNP
cord-268139-tgpsu4qz	88	7	for	for	IN
cord-268139-tgpsu4qz	88	8	the	the	DT
cord-268139-tgpsu4qz	88	9	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	88	10	-	-	HYPH
cord-268139-tgpsu4qz	88	11	terminal	terminal	NN
cord-268139-tgpsu4qz	88	12	truncation	truncation	NN
cord-268139-tgpsu4qz	88	13	mutant	mutant	NN
cord-268139-tgpsu4qz	88	14	(	(	-LRB-
cord-268139-tgpsu4qz	88	15	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	88	16	)	)	-RRB-
cord-268139-tgpsu4qz	88	17	.	.	.
cord-268139-tgpsu4qz	89	1	Clarified	clarify	VBN
cord-268139-tgpsu4qz	89	2	cell	cell	NN
cord-268139-tgpsu4qz	89	3	culture	culture	NN
cord-268139-tgpsu4qz	89	4	media	medium	NNS
cord-268139-tgpsu4qz	89	5	harvested	harvest	VBN
cord-268139-tgpsu4qz	89	6	from	from	IN
cord-268139-tgpsu4qz	89	7	cells	cell	NNS
cord-268139-tgpsu4qz	89	8	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	89	9	with	with	IN
cord-268139-tgpsu4qz	89	10	either	either	DT
cord-268139-tgpsu4qz	89	11	icwt	icwt	NNP
cord-268139-tgpsu4qz	89	12	or	or	CC
cord-268139-tgpsu4qz	89	13	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	89	14	RNA	RNA	NNP
cord-268139-tgpsu4qz	89	15	were	be	VBD
cord-268139-tgpsu4qz	89	16	capable	capable	JJ
cord-268139-tgpsu4qz	89	17	of	of	IN
cord-268139-tgpsu4qz	89	18	initiating	initiate	VBG
cord-268139-tgpsu4qz	89	19	a	a	DT
cord-268139-tgpsu4qz	89	20	productive	productive	JJ
cord-268139-tgpsu4qz	89	21	infection	infection	NN
cord-268139-tgpsu4qz	89	22	in	in	IN
cord-268139-tgpsu4qz	89	23	freshly	freshly	RB
cord-268139-tgpsu4qz	89	24	inoculated	inoculate	VBN
cord-268139-tgpsu4qz	89	25	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	89	26	cells	cell	NNS
cord-268139-tgpsu4qz	89	27	,	,	,
cord-268139-tgpsu4qz	89	28	demonstrating	demonstrate	VBG
cord-268139-tgpsu4qz	89	29	the	the	DT
cord-268139-tgpsu4qz	89	30	presence	presence	NN
cord-268139-tgpsu4qz	89	31	of	of	IN
cord-268139-tgpsu4qz	89	32	viable	viable	JJ
cord-268139-tgpsu4qz	89	33	virus	virus	NN
cord-268139-tgpsu4qz	89	34	.	.	.
cord-268139-tgpsu4qz	90	1	In	in	IN
cord-268139-tgpsu4qz	90	2	contrast	contrast	NN
cord-268139-tgpsu4qz	90	3	,	,	,
cord-268139-tgpsu4qz	90	4	cells	cell	NNS
cord-268139-tgpsu4qz	90	5	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	90	6	with	with	IN
cord-268139-tgpsu4qz	90	7	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	90	8	or	or	CC
cord-268139-tgpsu4qz	90	9	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	90	10	RNA	RNA	NNP
cord-268139-tgpsu4qz	90	11	showed	show	VBD
cord-268139-tgpsu4qz	90	12	foci	foci	NN
cord-268139-tgpsu4qz	90	13	containing	contain	VBG
cord-268139-tgpsu4qz	90	14	5	5	CD
cord-268139-tgpsu4qz	90	15	-10	-10	NN
cord-268139-tgpsu4qz	90	16	cell	cell	NN
cord-268139-tgpsu4qz	90	17	nuclei	nucleus	NNS
cord-268139-tgpsu4qz	90	18	at	at	IN
cord-268139-tgpsu4qz	90	19	72	72	CD
cord-268139-tgpsu4qz	90	20	-96	-96	SYM
cord-268139-tgpsu4qz	90	21	h	h	NNP
cord-268139-tgpsu4qz	90	22	p.e	p.e	NNP
cord-268139-tgpsu4qz	90	23	.	.	NNP
cord-268139-tgpsu4qz	90	24	,	,	,
cord-268139-tgpsu4qz	90	25	but	but	CC
cord-268139-tgpsu4qz	90	26	these	these	DT
cord-268139-tgpsu4qz	90	27	multi	multi	JJ
cord-268139-tgpsu4qz	90	28	-	-	JJ
cord-268139-tgpsu4qz	90	29	nucleated	nucleated	JJ
cord-268139-tgpsu4qz	90	30	foci	focus	NNS
cord-268139-tgpsu4qz	90	31	did	do	VBD
cord-268139-tgpsu4qz	90	32	not	not	RB
cord-268139-tgpsu4qz	90	33	progress	progress	VB
cord-268139-tgpsu4qz	90	34	or	or	CC
cord-268139-tgpsu4qz	90	35	spread	spread	VB
cord-268139-tgpsu4qz	90	36	over	over	IN
cord-268139-tgpsu4qz	90	37	time	time	NN
cord-268139-tgpsu4qz	90	38	like	like	IN
cord-268139-tgpsu4qz	90	39	wild	wild	JJ
cord-268139-tgpsu4qz	90	40	-	-	HYPH
cord-268139-tgpsu4qz	90	41	type	type	NN
cord-268139-tgpsu4qz	90	42	MHV	MHV	NNP
cord-268139-tgpsu4qz	90	43	syncytia	syncytia	NN
cord-268139-tgpsu4qz	90	44	.	.	.
cord-268139-tgpsu4qz	91	1	The	the	DT
cord-268139-tgpsu4qz	91	2	clarified	clarify	VBN
cord-268139-tgpsu4qz	91	3	media	medium	NNS
cord-268139-tgpsu4qz	91	4	from	from	IN
cord-268139-tgpsu4qz	91	5	cells	cell	NNS
cord-268139-tgpsu4qz	91	6	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	91	7	with	with	IN
cord-268139-tgpsu4qz	91	8	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	91	9	or	or	CC
cord-268139-tgpsu4qz	91	10	nsp1DMid	nsp1dmid	NN
cord-268139-tgpsu4qz	91	11	genomes	genome	NNS
cord-268139-tgpsu4qz	91	12	were	be	VBD
cord-268139-tgpsu4qz	91	13	not	not	RB
cord-268139-tgpsu4qz	91	14	capable	capable	JJ
cord-268139-tgpsu4qz	91	15	of	of	IN
cord-268139-tgpsu4qz	91	16	producing	produce	VBG
cord-268139-tgpsu4qz	91	17	CPE	CPE	NNP
cord-268139-tgpsu4qz	91	18	in	in	IN
cord-268139-tgpsu4qz	91	19	freshly	freshly	RB
cord-268139-tgpsu4qz	91	20	inoculated	inoculate	VBN
cord-268139-tgpsu4qz	91	21	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	91	22	cells	cell	NNS
cord-268139-tgpsu4qz	91	23	.	.	.
cord-268139-tgpsu4qz	92	1	This	this	DT
cord-268139-tgpsu4qz	92	2	result	result	NN
cord-268139-tgpsu4qz	92	3	indicated	indicate	VBD
cord-268139-tgpsu4qz	92	4	that	that	IN
cord-268139-tgpsu4qz	92	5	nsp1DFL	nsp1dfl	NN
cord-268139-tgpsu4qz	92	6	and	and	CC
cord-268139-tgpsu4qz	92	7	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	92	8	were	be	VBD
cord-268139-tgpsu4qz	92	9	not	not	RB
cord-268139-tgpsu4qz	92	10	capable	capable	JJ
cord-268139-tgpsu4qz	92	11	of	of	IN
cord-268139-tgpsu4qz	92	12	completing	complete	VBG
cord-268139-tgpsu4qz	92	13	a	a	DT
cord-268139-tgpsu4qz	92	14	productive	productive	JJ
cord-268139-tgpsu4qz	92	15	life	life	NN
cord-268139-tgpsu4qz	92	16	cycle	cycle	NN
cord-268139-tgpsu4qz	92	17	,	,	,
cord-268139-tgpsu4qz	92	18	defined	define	VBN
cord-268139-tgpsu4qz	92	19	by	by	IN
cord-268139-tgpsu4qz	92	20	the	the	DT
cord-268139-tgpsu4qz	92	21	lack	lack	NN
cord-268139-tgpsu4qz	92	22	of	of	IN
cord-268139-tgpsu4qz	92	23	infectious	infectious	JJ
cord-268139-tgpsu4qz	92	24	virus	virus	NN
cord-268139-tgpsu4qz	92	25	particles	particle	NNS
cord-268139-tgpsu4qz	92	26	in	in	IN
cord-268139-tgpsu4qz	92	27	the	the	DT
cord-268139-tgpsu4qz	92	28	cell	cell	NN
cord-268139-tgpsu4qz	92	29	media	medium	NNS
cord-268139-tgpsu4qz	92	30	supernatant	supernatant	NN
cord-268139-tgpsu4qz	92	31	following	follow	VBG
cord-268139-tgpsu4qz	92	32	electroporation	electroporation	NN
cord-268139-tgpsu4qz	92	33	.	.	.
cord-268139-tgpsu4qz	93	1	Nsp1DFL	Nsp1DFL	NNP
cord-268139-tgpsu4qz	94	1	and	and	CC
cord-268139-tgpsu4qz	94	2	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	94	3	RNA	RNA	NNP
cord-268139-tgpsu4qz	94	4	molecules	molecule	NNS
cord-268139-tgpsu4qz	94	5	were	be	VBD
cord-268139-tgpsu4qz	94	6	tested	test	VBN
cord-268139-tgpsu4qz	94	7	for	for	IN
cord-268139-tgpsu4qz	94	8	their	-PRON-	PRP$
cord-268139-tgpsu4qz	94	9	capacity	capacity	NN
cord-268139-tgpsu4qz	94	10	to	to	TO
cord-268139-tgpsu4qz	94	11	produce	produce	VB
cord-268139-tgpsu4qz	94	12	viruses	virus	NNS
cord-268139-tgpsu4qz	94	13	in	in	IN
cord-268139-tgpsu4qz	94	14	three	three	CD
cord-268139-tgpsu4qz	94	15	independent	independent	JJ
cord-268139-tgpsu4qz	94	16	experiments	experiment	NNS
cord-268139-tgpsu4qz	94	17	,	,	,
cord-268139-tgpsu4qz	94	18	all	all	DT
cord-268139-tgpsu4qz	94	19	of	of	IN
cord-268139-tgpsu4qz	94	20	which	which	WDT
cord-268139-tgpsu4qz	94	21	yielded	yield	VBD
cord-268139-tgpsu4qz	94	22	this	this	DT
cord-268139-tgpsu4qz	94	23	same	same	JJ
cord-268139-tgpsu4qz	94	24	result	result	NN
cord-268139-tgpsu4qz	94	25	.	.	.
cord-268139-tgpsu4qz	95	1	The	the	DT
cord-268139-tgpsu4qz	95	2	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	95	3	virus	virus	NN
cord-268139-tgpsu4qz	95	4	was	be	VBD
cord-268139-tgpsu4qz	95	5	purified	purify	VBN
cord-268139-tgpsu4qz	95	6	by	by	IN
cord-268139-tgpsu4qz	95	7	three	three	CD
cord-268139-tgpsu4qz	95	8	rounds	round	NNS
cord-268139-tgpsu4qz	95	9	of	of	IN
cord-268139-tgpsu4qz	95	10	plaque	plaque	NN
cord-268139-tgpsu4qz	95	11	isolation	isolation	NN
cord-268139-tgpsu4qz	95	12	,	,	,
cord-268139-tgpsu4qz	95	13	and	and	CC
cord-268139-tgpsu4qz	95	14	RT	RT	NNP
cord-268139-tgpsu4qz	95	15	-	-	HYPH
cord-268139-tgpsu4qz	95	16	PCR	PCR	NNP
cord-268139-tgpsu4qz	95	17	was	be	VBD
cord-268139-tgpsu4qz	95	18	used	use	VBN
cord-268139-tgpsu4qz	95	19	to	to	TO
cord-268139-tgpsu4qz	95	20	sequence	sequence	VB
cord-268139-tgpsu4qz	95	21	across	across	IN
cord-268139-tgpsu4qz	95	22	the	the	DT
cord-268139-tgpsu4qz	95	23	entire	entire	JJ
cord-268139-tgpsu4qz	95	24	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	95	25	region	region	NN
cord-268139-tgpsu4qz	95	26	.	.	.
cord-268139-tgpsu4qz	96	1	The	the	DT
cord-268139-tgpsu4qz	96	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	96	3	virus	virus	NN
cord-268139-tgpsu4qz	96	4	maintained	maintain	VBD
cord-268139-tgpsu4qz	96	5	the	the	DT
cord-268139-tgpsu4qz	96	6	engineered	engineer	VBN
cord-268139-tgpsu4qz	96	7	deletion	deletion	NN
cord-268139-tgpsu4qz	96	8	and	and	CC
cord-268139-tgpsu4qz	96	9	had	have	VBD
cord-268139-tgpsu4qz	96	10	no	no	DT
cord-268139-tgpsu4qz	96	11	other	other	JJ
cord-268139-tgpsu4qz	96	12	mutations	mutation	NNS
cord-268139-tgpsu4qz	96	13	within	within	IN
cord-268139-tgpsu4qz	96	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	96	15	.	.	.
cord-268139-tgpsu4qz	97	1	These	these	DT
cord-268139-tgpsu4qz	97	2	results	result	NNS
cord-268139-tgpsu4qz	97	3	demonstrate	demonstrate	VBP
cord-268139-tgpsu4qz	97	4	that	that	IN
cord-268139-tgpsu4qz	97	5	the	the	DT
cord-268139-tgpsu4qz	97	6	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	97	7	-	-	HYPH
cord-268139-tgpsu4qz	97	8	terminal	terminal	NN
cord-268139-tgpsu4qz	97	9	half	half	NN
cord-268139-tgpsu4qz	97	10	of	of	IN
cord-268139-tgpsu4qz	97	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	97	12	is	be	VBZ
cord-268139-tgpsu4qz	97	13	dispensable	dispensable	JJ
cord-268139-tgpsu4qz	97	14	for	for	IN
cord-268139-tgpsu4qz	97	15	MHV	MHV	NNP
cord-268139-tgpsu4qz	97	16	replication	replication	NN
cord-268139-tgpsu4qz	97	17	and	and	CC
cord-268139-tgpsu4qz	97	18	suggest	suggest	VBP
cord-268139-tgpsu4qz	97	19	that	that	IN
cord-268139-tgpsu4qz	97	20	deletion	deletion	NN
cord-268139-tgpsu4qz	97	21	of	of	IN
cord-268139-tgpsu4qz	97	22	residues	residue	NNS
cord-268139-tgpsu4qz	97	23	in	in	IN
cord-268139-tgpsu4qz	97	24	the	the	DT
cord-268139-tgpsu4qz	97	25	amino	amino	NN
cord-268139-tgpsu4qz	97	26	-	-	HYPH
cord-268139-tgpsu4qz	97	27	terminal	terminal	NN
cord-268139-tgpsu4qz	97	28	half	half	NN
cord-268139-tgpsu4qz	97	29	is	be	VBZ
cord-268139-tgpsu4qz	97	30	not	not	RB
cord-268139-tgpsu4qz	97	31	tolerated	tolerate	VBN
cord-268139-tgpsu4qz	97	32	for	for	IN
cord-268139-tgpsu4qz	97	33	a	a	DT
cord-268139-tgpsu4qz	97	34	productive	productive	JJ
cord-268139-tgpsu4qz	97	35	infection	infection	NN
cord-268139-tgpsu4qz	97	36	.	.	.
cord-268139-tgpsu4qz	98	1	Charge	charge	NN
cord-268139-tgpsu4qz	98	2	-	-	HYPH
cord-268139-tgpsu4qz	98	3	to	to	IN
cord-268139-tgpsu4qz	98	4	-	-	HYPH
cord-268139-tgpsu4qz	98	5	alanine	alanine	NN
cord-268139-tgpsu4qz	98	6	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	98	7	of	of	IN
cord-268139-tgpsu4qz	98	8	the	the	DT
cord-268139-tgpsu4qz	98	9	nsp1	nsp1	CD
cord-268139-tgpsu4qz	98	10	amino	amino	NN
cord-268139-tgpsu4qz	98	11	-	-	HYPH
cord-268139-tgpsu4qz	98	12	terminus	terminus	NN
cord-268139-tgpsu4qz	99	1	The	the	DT
cord-268139-tgpsu4qz	99	2	fact	fact	NN
cord-268139-tgpsu4qz	99	3	that	that	IN
cord-268139-tgpsu4qz	99	4	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	99	5	and	and	CC
cord-268139-tgpsu4qz	99	6	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	99	7	did	do	VBD
cord-268139-tgpsu4qz	99	8	not	not	RB
cord-268139-tgpsu4qz	99	9	generate	generate	VB
cord-268139-tgpsu4qz	99	10	productive	productive	JJ
cord-268139-tgpsu4qz	99	11	infections	infection	NNS
cord-268139-tgpsu4qz	99	12	suggests	suggest	VBZ
cord-268139-tgpsu4qz	99	13	that	that	IN
cord-268139-tgpsu4qz	99	14	this	this	DT
cord-268139-tgpsu4qz	99	15	region	region	NN
cord-268139-tgpsu4qz	99	16	of	of	IN
cord-268139-tgpsu4qz	99	17	the	the	DT
cord-268139-tgpsu4qz	99	18	protein	protein	NN
cord-268139-tgpsu4qz	99	19	has	have	VBZ
cord-268139-tgpsu4qz	99	20	a	a	DT
cord-268139-tgpsu4qz	99	21	critical	critical	JJ
cord-268139-tgpsu4qz	99	22	function	function	NN
cord-268139-tgpsu4qz	99	23	in	in	IN
cord-268139-tgpsu4qz	99	24	MHV	MHV	NNP
cord-268139-tgpsu4qz	99	25	replication	replication	NN
cord-268139-tgpsu4qz	99	26	.	.	.
cord-268139-tgpsu4qz	100	1	However	however	RB
cord-268139-tgpsu4qz	100	2	,	,	,
cord-268139-tgpsu4qz	100	3	it	-PRON-	PRP
cord-268139-tgpsu4qz	100	4	is	be	VBZ
cord-268139-tgpsu4qz	100	5	also	also	RB
cord-268139-tgpsu4qz	100	6	possible	possible	JJ
cord-268139-tgpsu4qz	100	7	that	that	IN
cord-268139-tgpsu4qz	100	8	the	the	DT
cord-268139-tgpsu4qz	100	9	deletions	deletion	NNS
cord-268139-tgpsu4qz	100	10	removed	remove	VBD
cord-268139-tgpsu4qz	100	11	required	require	VBN
cord-268139-tgpsu4qz	100	12	RNA	RNA	NNP
cord-268139-tgpsu4qz	100	13	elements	element	NNS
cord-268139-tgpsu4qz	100	14	within	within	IN
cord-268139-tgpsu4qz	100	15	the	the	DT
cord-268139-tgpsu4qz	100	16	5V	5v	JJ
cord-268139-tgpsu4qz	100	17	end	end	NN
cord-268139-tgpsu4qz	100	18	of	of	IN
cord-268139-tgpsu4qz	100	19	the	the	DT
cord-268139-tgpsu4qz	100	20	nsp1coding	nsp1coding	NNP
cord-268139-tgpsu4qz	100	21	sequence	sequence	NN
cord-268139-tgpsu4qz	100	22	.	.	.
cord-268139-tgpsu4qz	101	1	To	to	TO
cord-268139-tgpsu4qz	101	2	investigate	investigate	VB
cord-268139-tgpsu4qz	101	3	the	the	DT
cord-268139-tgpsu4qz	101	4	function	function	NN
cord-268139-tgpsu4qz	101	5	of	of	IN
cord-268139-tgpsu4qz	101	6	the	the	DT
cord-268139-tgpsu4qz	101	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	101	8	amino	amino	NN
cord-268139-tgpsu4qz	101	9	-	-	HYPH
cord-268139-tgpsu4qz	101	10	terminus	terminus	NN
cord-268139-tgpsu4qz	101	11	with	with	IN
cord-268139-tgpsu4qz	101	12	minimal	minimal	JJ
cord-268139-tgpsu4qz	101	13	disruption	disruption	NN
cord-268139-tgpsu4qz	101	14	of	of	IN
cord-268139-tgpsu4qz	101	15	the	the	DT
cord-268139-tgpsu4qz	101	16	correspond	correspond	VB
cord-268139-tgpsu4qz	101	17	-	-	HYPH
cord-268139-tgpsu4qz	101	18	ing	e	VBG
cord-268139-tgpsu4qz	101	19	RNA	RNA	NNP
cord-268139-tgpsu4qz	101	20	sequence	sequence	NN
cord-268139-tgpsu4qz	101	21	,	,	,
cord-268139-tgpsu4qz	101	22	viruses	virus	NNS
cord-268139-tgpsu4qz	101	23	were	be	VBD
cord-268139-tgpsu4qz	101	24	engineered	engineer	VBN
cord-268139-tgpsu4qz	101	25	to	to	TO
cord-268139-tgpsu4qz	101	26	contain	contain	VB
cord-268139-tgpsu4qz	101	27	point	point	NN
cord-268139-tgpsu4qz	101	28	mutations	mutation	NNS
cord-268139-tgpsu4qz	101	29	within	within	IN
cord-268139-tgpsu4qz	101	30	this	this	DT
cord-268139-tgpsu4qz	101	31	region	region	NN
cord-268139-tgpsu4qz	101	32	.	.	.
cord-268139-tgpsu4qz	102	1	Mutations	mutation	NNS
cord-268139-tgpsu4qz	102	2	were	be	VBD
cord-268139-tgpsu4qz	102	3	introduced	introduce	VBN
cord-268139-tgpsu4qz	102	4	into	into	IN
cord-268139-tgpsu4qz	103	1	the	the	DT
cord-268139-tgpsu4qz	103	2	icMHV	icMHV	NNP
cord-268139-tgpsu4qz	103	3	fragment	fragment	NN
cord-268139-tgpsu4qz	103	4	A	a	DT
cord-268139-tgpsu4qz	103	5	plasmid	plasmid	NN
cord-268139-tgpsu4qz	103	6	using	use	VBG
cord-268139-tgpsu4qz	103	7	sitedirected	sitedirected	JJ
cord-268139-tgpsu4qz	103	8	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	103	9	.	.	.
cord-268139-tgpsu4qz	104	1	Single	single	JJ
cord-268139-tgpsu4qz	104	2	or	or	CC
cord-268139-tgpsu4qz	104	3	paired	pair	VBN
cord-268139-tgpsu4qz	104	4	charged	charge	VBN
cord-268139-tgpsu4qz	104	5	residues	residue	NNS
cord-268139-tgpsu4qz	104	6	(	(	-LRB-
cord-268139-tgpsu4qz	104	7	D	D	NNP
cord-268139-tgpsu4qz	104	8	,	,	,
cord-268139-tgpsu4qz	104	9	E	E	NNP
cord-268139-tgpsu4qz	104	10	,	,	,
cord-268139-tgpsu4qz	104	11	R	r	NN
cord-268139-tgpsu4qz	104	12	,	,	,
cord-268139-tgpsu4qz	104	13	K	K	NNP
cord-268139-tgpsu4qz	104	14	,	,	,
cord-268139-tgpsu4qz	104	15	and	and	CC
cord-268139-tgpsu4qz	104	16	H	H	NNP
cord-268139-tgpsu4qz	104	17	)	)	-RRB-
cord-268139-tgpsu4qz	104	18	within	within	IN
cord-268139-tgpsu4qz	104	19	the	the	DT
cord-268139-tgpsu4qz	104	20	amino	amino	NN
cord-268139-tgpsu4qz	104	21	-	-	HYPH
cord-268139-tgpsu4qz	104	22	terminal	terminal	NN
cord-268139-tgpsu4qz	104	23	half	half	NN
cord-268139-tgpsu4qz	104	24	of	of	IN
cord-268139-tgpsu4qz	104	25	MHV	MHV	NNP
cord-268139-tgpsu4qz	104	26	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	104	27	that	that	WDT
cord-268139-tgpsu4qz	104	28	are	be	VBP
cord-268139-tgpsu4qz	104	29	conserved	conserve	VBN
cord-268139-tgpsu4qz	104	30	across	across	IN
cord-268139-tgpsu4qz	104	31	all	all	DT
cord-268139-tgpsu4qz	104	32	group	group	NN
cord-268139-tgpsu4qz	104	33	2	2	CD
cord-268139-tgpsu4qz	104	34	coronaviruses	coronaviruse	NNS
cord-268139-tgpsu4qz	104	35	were	be	VBD
cord-268139-tgpsu4qz	104	36	substituted	substitute	VBN
cord-268139-tgpsu4qz	104	37	with	with	IN
cord-268139-tgpsu4qz	104	38	alanine	alanine	NN
cord-268139-tgpsu4qz	104	39	(	(	-LRB-
cord-268139-tgpsu4qz	104	40	Fig	Fig	NNP
cord-268139-tgpsu4qz	104	41	.	.	.
cord-268139-tgpsu4qz	104	42	1B	1b	CD
cord-268139-tgpsu4qz	104	43	)	)	-RRB-
cord-268139-tgpsu4qz	104	44	.	.	.
cord-268139-tgpsu4qz	105	1	Mutated	mutate	VBN
cord-268139-tgpsu4qz	106	1	fragment	fragment	VB
cord-268139-tgpsu4qz	106	2	A	a	DT
cord-268139-tgpsu4qz	106	3	cDNAs	cdna	NNS
cord-268139-tgpsu4qz	106	4	were	be	VBD
cord-268139-tgpsu4qz	106	5	used	use	VBN
cord-268139-tgpsu4qz	106	6	as	as	IN
cord-268139-tgpsu4qz	106	7	before	before	RB
cord-268139-tgpsu4qz	106	8	to	to	TO
cord-268139-tgpsu4qz	106	9	generate	generate	VB
cord-268139-tgpsu4qz	106	10	full	full	JJ
cord-268139-tgpsu4qz	106	11	-	-	HYPH
cord-268139-tgpsu4qz	106	12	length	length	NN
cord-268139-tgpsu4qz	106	13	genomic	genomic	JJ
cord-268139-tgpsu4qz	106	14	viral	viral	JJ
cord-268139-tgpsu4qz	106	15	RNA	RNA	NNP
cord-268139-tgpsu4qz	106	16	.	.	.
cord-268139-tgpsu4qz	107	1	Virus	virus	NN
cord-268139-tgpsu4qz	107	2	-	-	HYPH
cord-268139-tgpsu4qz	107	3	induced	induce	VBN
cord-268139-tgpsu4qz	107	4	syncytia	syncytia	NN
cord-268139-tgpsu4qz	107	5	were	be	VBD
cord-268139-tgpsu4qz	107	6	detected	detect	VBN
cord-268139-tgpsu4qz	107	7	within	within	IN
cord-268139-tgpsu4qz	107	8	24	24	CD
cord-268139-tgpsu4qz	107	9	h	h	NN
cord-268139-tgpsu4qz	107	10	in	in	IN
cord-268139-tgpsu4qz	107	11	BHK	BHK	NNP
cord-268139-tgpsu4qz	107	12	-	-	HYPH
cord-268139-tgpsu4qz	107	13	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	107	14	cells	cell	NNS
cord-268139-tgpsu4qz	107	15	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	107	16	with	with	IN
cord-268139-tgpsu4qz	107	17	either	either	DT
cord-268139-tgpsu4qz	107	18	icwt	icwt	NNP
cord-268139-tgpsu4qz	107	19	RNA	RNA	NNP
cord-268139-tgpsu4qz	107	20	or	or	CC
cord-268139-tgpsu4qz	107	21	with	with	IN
cord-268139-tgpsu4qz	107	22	RNA	RNA	NNP
cord-268139-tgpsu4qz	107	23	for	for	IN
cord-268139-tgpsu4qz	107	24	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	107	25	mutants	mutant	NNS
cord-268139-tgpsu4qz	107	26	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	107	27	,	,	,
cord-268139-tgpsu4qz	107	28	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	107	29	,	,	,
cord-268139-tgpsu4qz	108	1	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	108	2	,	,	,
cord-268139-tgpsu4qz	108	3	or	or	CC
cord-268139-tgpsu4qz	108	4	VUSB7	vusb7	NN
cord-268139-tgpsu4qz	108	5	.	.	.
cord-268139-tgpsu4qz	109	1	The	the	DT
cord-268139-tgpsu4qz	109	2	timing	timing	NN
cord-268139-tgpsu4qz	109	3	and	and	CC
cord-268139-tgpsu4qz	109	4	extent	extent	NN
cord-268139-tgpsu4qz	109	5	of	of	IN
cord-268139-tgpsu4qz	109	6	CPE	CPE	NNP
cord-268139-tgpsu4qz	109	7	following	follow	VBG
cord-268139-tgpsu4qz	109	8	transfection	transfection	NN
cord-268139-tgpsu4qz	109	9	with	with	IN
cord-268139-tgpsu4qz	109	10	these	these	DT
cord-268139-tgpsu4qz	109	11	mutants	mutant	NNS
cord-268139-tgpsu4qz	109	12	were	be	VBD
cord-268139-tgpsu4qz	109	13	indistinguishable	indistinguishable	JJ
cord-268139-tgpsu4qz	109	14	from	from	IN
cord-268139-tgpsu4qz	109	15	icwt	icwt	NNP
cord-268139-tgpsu4qz	109	16	.	.	.
cord-268139-tgpsu4qz	110	1	Cells	cell	NNS
cord-268139-tgpsu4qz	110	2	transfected	transfecte	VBN
cord-268139-tgpsu4qz	110	3	with	with	IN
cord-268139-tgpsu4qz	110	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	110	5	for	for	IN
cord-268139-tgpsu4qz	110	6	VUSB4	VUSB4	NNS
cord-268139-tgpsu4qz	110	7	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	110	8	CPE	CPE	NNP
cord-268139-tgpsu4qz	110	9	at	at	IN
cord-268139-tgpsu4qz	110	10	72	72	CD
cord-268139-tgpsu4qz	110	11	h	h	NN
cord-268139-tgpsu4qz	110	12	p.e	p.e	NNP
cord-268139-tgpsu4qz	110	13	.	.	NNP
cord-268139-tgpsu4qz	110	14	,	,	,
cord-268139-tgpsu4qz	110	15	similar	similar	JJ
cord-268139-tgpsu4qz	110	16	to	to	IN
cord-268139-tgpsu4qz	110	17	the	the	DT
cord-268139-tgpsu4qz	110	18	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	110	19	observed	observe	VBN
cord-268139-tgpsu4qz	110	20	with	with	IN
cord-268139-tgpsu4qz	110	21	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	110	22	.	.	.
cord-268139-tgpsu4qz	111	1	The	the	DT
cord-268139-tgpsu4qz	111	2	clarified	clarify	VBN
cord-268139-tgpsu4qz	111	3	media	medium	NNS
cord-268139-tgpsu4qz	111	4	supernatants	supernatant	NNS
cord-268139-tgpsu4qz	111	5	from	from	IN
cord-268139-tgpsu4qz	111	6	cells	cell	NNS
cord-268139-tgpsu4qz	111	7	transfected	transfecte	VBN
cord-268139-tgpsu4qz	111	8	with	with	IN
cord-268139-tgpsu4qz	111	9	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	111	10	,	,	,
cord-268139-tgpsu4qz	111	11	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	111	12	,	,	,
cord-268139-tgpsu4qz	111	13	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	111	14	,	,	,
cord-268139-tgpsu4qz	111	15	VUSB4	VUSB4	NNS
cord-268139-tgpsu4qz	111	16	,	,	,
cord-268139-tgpsu4qz	111	17	or	or	CC
cord-268139-tgpsu4qz	111	18	VUSB7	vusb7	NN
cord-268139-tgpsu4qz	112	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	112	2	were	be	VBD
cord-268139-tgpsu4qz	112	3	capable	capable	JJ
cord-268139-tgpsu4qz	112	4	of	of	IN
cord-268139-tgpsu4qz	112	5	infecting	infect	VBG
cord-268139-tgpsu4qz	112	6	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	112	7	cells	cell	NNS
cord-268139-tgpsu4qz	112	8	,	,	,
cord-268139-tgpsu4qz	112	9	indicating	indicate	VBG
cord-268139-tgpsu4qz	112	10	that	that	IN
cord-268139-tgpsu4qz	112	11	they	-PRON-	PRP
cord-268139-tgpsu4qz	112	12	contained	contain	VBD
cord-268139-tgpsu4qz	112	13	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	112	14	mutant	mutant	JJ
cord-268139-tgpsu4qz	112	15	viruses	virus	NNS
cord-268139-tgpsu4qz	112	16	.	.	.
cord-268139-tgpsu4qz	113	1	As	as	IN
cord-268139-tgpsu4qz	113	2	with	with	IN
cord-268139-tgpsu4qz	113	3	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	113	4	,	,	,
cord-268139-tgpsu4qz	113	5	the	the	DT
cord-268139-tgpsu4qz	113	6	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	113	7	point	point	NN
cord-268139-tgpsu4qz	113	8	mutants	mutant	NNS
cord-268139-tgpsu4qz	113	9	were	be	VBD
cord-268139-tgpsu4qz	113	10	purified	purify	VBN
cord-268139-tgpsu4qz	113	11	by	by	IN
cord-268139-tgpsu4qz	113	12	three	three	CD
cord-268139-tgpsu4qz	113	13	rounds	round	NNS
cord-268139-tgpsu4qz	113	14	of	of	IN
cord-268139-tgpsu4qz	113	15	plaque	plaque	NN
cord-268139-tgpsu4qz	113	16	isolation	isolation	NN
cord-268139-tgpsu4qz	113	17	,	,	,
cord-268139-tgpsu4qz	113	18	and	and	CC
cord-268139-tgpsu4qz	113	19	RT	RT	NNP
cord-268139-tgpsu4qz	113	20	-	-	HYPH
cord-268139-tgpsu4qz	113	21	PCR	PCR	NNP
cord-268139-tgpsu4qz	113	22	was	be	VBD
cord-268139-tgpsu4qz	113	23	used	use	VBN
cord-268139-tgpsu4qz	113	24	to	to	TO
cord-268139-tgpsu4qz	113	25	sequence	sequence	VB
cord-268139-tgpsu4qz	113	26	across	across	IN
cord-268139-tgpsu4qz	113	27	the	the	DT
cord-268139-tgpsu4qz	113	28	entire	entire	JJ
cord-268139-tgpsu4qz	113	29	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	113	30	region	region	NN
cord-268139-tgpsu4qz	113	31	.	.	.
cord-268139-tgpsu4qz	114	1	The	the	DT
cord-268139-tgpsu4qz	114	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	114	3	viruses	virus	NNS
cord-268139-tgpsu4qz	114	4	maintained	maintain	VBD
cord-268139-tgpsu4qz	114	5	the	the	DT
cord-268139-tgpsu4qz	114	6	engineered	engineer	VBN
cord-268139-tgpsu4qz	114	7	changes	change	NNS
cord-268139-tgpsu4qz	114	8	and	and	CC
cord-268139-tgpsu4qz	114	9	lacked	lack	VBD
cord-268139-tgpsu4qz	114	10	other	other	JJ
cord-268139-tgpsu4qz	114	11	mutations	mutation	NNS
cord-268139-tgpsu4qz	114	12	within	within	IN
cord-268139-tgpsu4qz	114	13	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	114	14	.	.	.
cord-268139-tgpsu4qz	115	1	Similar	similar	JJ
cord-268139-tgpsu4qz	115	2	to	to	IN
cord-268139-tgpsu4qz	115	3	the	the	DT
cord-268139-tgpsu4qz	115	4	results	result	NNS
cord-268139-tgpsu4qz	115	5	with	with	IN
cord-268139-tgpsu4qz	115	6	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	115	7	and	and	CC
cord-268139-tgpsu4qz	115	8	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	115	9	,	,	,
cord-268139-tgpsu4qz	115	10	cells	cell	NNS
cord-268139-tgpsu4qz	115	11	transfected	transfecte	VBN
cord-268139-tgpsu4qz	115	12	with	with	IN
cord-268139-tgpsu4qz	115	13	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	115	14	or	or	CC
cord-268139-tgpsu4qz	115	15	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	115	16	RNA	RNA	NNP
cord-268139-tgpsu4qz	115	17	produced	produce	VBD
cord-268139-tgpsu4qz	115	18	foci	foci	NN
cord-268139-tgpsu4qz	115	19	containing	contain	VBG
cord-268139-tgpsu4qz	115	20	5	5	CD
cord-268139-tgpsu4qz	115	21	-10	-10	NN
cord-268139-tgpsu4qz	115	22	cell	cell	NN
cord-268139-tgpsu4qz	115	23	nuclei	nucleus	NNS
cord-268139-tgpsu4qz	115	24	at	at	IN
cord-268139-tgpsu4qz	115	25	72	72	CD
cord-268139-tgpsu4qz	115	26	-96	-96	SYM
cord-268139-tgpsu4qz	115	27	h	h	NNP
cord-268139-tgpsu4qz	115	28	p.e	p.e	NNP
cord-268139-tgpsu4qz	115	29	,	,	,
cord-268139-tgpsu4qz	115	30	but	but	CC
cord-268139-tgpsu4qz	115	31	the	the	DT
cord-268139-tgpsu4qz	115	32	extent	extent	NN
cord-268139-tgpsu4qz	115	33	of	of	IN
cord-268139-tgpsu4qz	115	34	these	these	DT
cord-268139-tgpsu4qz	115	35	multi	multi	JJ
cord-268139-tgpsu4qz	115	36	-	-	JJ
cord-268139-tgpsu4qz	115	37	nucleated	nucleated	JJ
cord-268139-tgpsu4qz	115	38	foci	focus	NNS
cord-268139-tgpsu4qz	115	39	did	do	VBD
cord-268139-tgpsu4qz	115	40	not	not	RB
cord-268139-tgpsu4qz	115	41	increase	increase	VB
cord-268139-tgpsu4qz	115	42	over	over	IN
cord-268139-tgpsu4qz	115	43	time	time	NN
cord-268139-tgpsu4qz	115	44	.	.	.
cord-268139-tgpsu4qz	116	1	Moreover	moreover	RB
cord-268139-tgpsu4qz	116	2	,	,	,
cord-268139-tgpsu4qz	116	3	clarified	clarify	VBN
cord-268139-tgpsu4qz	116	4	media	media	NN
cord-268139-tgpsu4qz	116	5	supernatant	supernatant	NN
cord-268139-tgpsu4qz	116	6	harvested	harvest	VBN
cord-268139-tgpsu4qz	116	7	from	from	IN
cord-268139-tgpsu4qz	116	8	cells	cell	NNS
cord-268139-tgpsu4qz	116	9	transfected	transfecte	VBN
cord-268139-tgpsu4qz	116	10	with	with	IN
cord-268139-tgpsu4qz	116	11	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	116	12	or	or	CC
cord-268139-tgpsu4qz	116	13	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	116	14	RNA	RNA	NNP
cord-268139-tgpsu4qz	116	15	did	do	VBD
cord-268139-tgpsu4qz	116	16	not	not	RB
cord-268139-tgpsu4qz	116	17	generate	generate	VB
cord-268139-tgpsu4qz	116	18	a	a	DT
cord-268139-tgpsu4qz	116	19	productive	productive	JJ
cord-268139-tgpsu4qz	116	20	infection	infection	NN
cord-268139-tgpsu4qz	116	21	following	follow	VBG
cord-268139-tgpsu4qz	116	22	incubation	incubation	NN
cord-268139-tgpsu4qz	116	23	with	with	IN
cord-268139-tgpsu4qz	116	24	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	116	25	cells	cell	NNS
cord-268139-tgpsu4qz	116	26	,	,	,
cord-268139-tgpsu4qz	116	27	indicating	indicate	VBG
cord-268139-tgpsu4qz	116	28	that	that	IN
cord-268139-tgpsu4qz	116	29	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	116	30	and	and	CC
cord-268139-tgpsu4qz	116	31	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	116	32	were	be	VBD
cord-268139-tgpsu4qz	116	33	blocked	block	VBN
cord-268139-tgpsu4qz	116	34	at	at	IN
cord-268139-tgpsu4qz	116	35	some	some	DT
cord-268139-tgpsu4qz	116	36	stage	stage	NN
cord-268139-tgpsu4qz	116	37	in	in	IN
cord-268139-tgpsu4qz	116	38	the	the	DT
cord-268139-tgpsu4qz	116	39	viral	viral	JJ
cord-268139-tgpsu4qz	116	40	life	life	NN
cord-268139-tgpsu4qz	116	41	cycle	cycle	NN
cord-268139-tgpsu4qz	116	42	.	.	.
cord-268139-tgpsu4qz	117	1	VUSB5	VUSB5	NNP
cord-268139-tgpsu4qz	117	2	and	and	CC
cord-268139-tgpsu4qz	117	3	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	117	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	117	5	molecule	molecule	NN
cord-268139-tgpsu4qz	117	6	were	be	VBD
cord-268139-tgpsu4qz	117	7	assayed	assay	VBN
cord-268139-tgpsu4qz	117	8	for	for	IN
cord-268139-tgpsu4qz	117	9	their	-PRON-	PRP$
cord-268139-tgpsu4qz	117	10	capacity	capacity	NN
cord-268139-tgpsu4qz	117	11	to	to	TO
cord-268139-tgpsu4qz	117	12	produce	produce	VB
cord-268139-tgpsu4qz	117	13	viruses	virus	NNS
cord-268139-tgpsu4qz	117	14	in	in	IN
cord-268139-tgpsu4qz	117	15	two	two	CD
cord-268139-tgpsu4qz	117	16	independent	independent	JJ
cord-268139-tgpsu4qz	117	17	experiments	experiment	NNS
cord-268139-tgpsu4qz	117	18	,	,	,
cord-268139-tgpsu4qz	117	19	both	both	DT
cord-268139-tgpsu4qz	117	20	of	of	IN
cord-268139-tgpsu4qz	117	21	which	which	WDT
cord-268139-tgpsu4qz	117	22	showed	show	VBD
cord-268139-tgpsu4qz	117	23	the	the	DT
cord-268139-tgpsu4qz	117	24	same	same	JJ
cord-268139-tgpsu4qz	117	25	result	result	NN
cord-268139-tgpsu4qz	117	26	.	.	.
cord-268139-tgpsu4qz	118	1	It	-PRON-	PRP
cord-268139-tgpsu4qz	118	2	has	have	VBZ
cord-268139-tgpsu4qz	118	3	been	be	VBN
cord-268139-tgpsu4qz	118	4	reported	report	VBN
cord-268139-tgpsu4qz	118	5	that	that	IN
cord-268139-tgpsu4qz	118	6	charge	charge	NN
cord-268139-tgpsu4qz	118	7	-	-	HYPH
cord-268139-tgpsu4qz	118	8	to	to	IN
cord-268139-tgpsu4qz	118	9	-	-	HYPH
cord-268139-tgpsu4qz	118	10	alanine	alanine	NN
cord-268139-tgpsu4qz	118	11	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	118	12	of	of	IN
cord-268139-tgpsu4qz	118	13	viral	viral	JJ
cord-268139-tgpsu4qz	118	14	proteins	protein	NNS
cord-268139-tgpsu4qz	118	15	may	may	MD
cord-268139-tgpsu4qz	118	16	generate	generate	VB
cord-268139-tgpsu4qz	118	17	temperaturesensitive	temperaturesensitive	JJ
cord-268139-tgpsu4qz	118	18	mutants	mutant	NNS
cord-268139-tgpsu4qz	118	19	(	(	-LRB-
cord-268139-tgpsu4qz	118	20	Hanley	Hanley	NNP
cord-268139-tgpsu4qz	118	21	et	et	NNP
cord-268139-tgpsu4qz	118	22	al	al	NNP
cord-268139-tgpsu4qz	118	23	.	.	NNP
cord-268139-tgpsu4qz	118	24	,	,	,
cord-268139-tgpsu4qz	118	25	2002	2002	CD
cord-268139-tgpsu4qz	118	26	;	;	:
cord-268139-tgpsu4qz	118	27	Hassett	Hassett	NNP
cord-268139-tgpsu4qz	118	28	and	and	CC
cord-268139-tgpsu4qz	118	29	Condit	Condit	NNP
cord-268139-tgpsu4qz	118	30	,	,	,
cord-268139-tgpsu4qz	118	31	1994	1994	CD
cord-268139-tgpsu4qz	118	32	;	;	:
cord-268139-tgpsu4qz	118	33	Tang	Tang	NNP
cord-268139-tgpsu4qz	118	34	et	et	NNP
cord-268139-tgpsu4qz	118	35	al	al	NNP
cord-268139-tgpsu4qz	118	36	.	.	NNP
cord-268139-tgpsu4qz	118	37	,	,	,
cord-268139-tgpsu4qz	118	38	2002	2002	CD
cord-268139-tgpsu4qz	118	39	)	)	-RRB-
cord-268139-tgpsu4qz	118	40	.	.	.
cord-268139-tgpsu4qz	119	1	In	in	IN
cord-268139-tgpsu4qz	119	2	the	the	DT
cord-268139-tgpsu4qz	119	3	current	current	JJ
cord-268139-tgpsu4qz	119	4	study	study	NN
cord-268139-tgpsu4qz	119	5	,	,	,
cord-268139-tgpsu4qz	119	6	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	119	7	mutants	mutant	NNS
cord-268139-tgpsu4qz	119	8	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	119	9	,	,	,
cord-268139-tgpsu4qz	119	10	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	119	11	,	,	,
cord-268139-tgpsu4qz	119	12	and	and	CC
cord-268139-tgpsu4qz	119	13	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	119	14	did	do	VBD
cord-268139-tgpsu4qz	119	15	not	not	RB
cord-268139-tgpsu4qz	119	16	exhibit	exhibit	VB
cord-268139-tgpsu4qz	119	17	temperature	temperature	NN
cord-268139-tgpsu4qz	119	18	-	-	HYPH
cord-268139-tgpsu4qz	119	19	sensitive	sensitive	JJ
cord-268139-tgpsu4qz	119	20	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	119	21	as	as	IN
cord-268139-tgpsu4qz	119	22	no	no	DT
cord-268139-tgpsu4qz	119	23	infectious	infectious	JJ
cord-268139-tgpsu4qz	119	24	virus	virus	NN
cord-268139-tgpsu4qz	119	25	was	be	VBD
cord-268139-tgpsu4qz	119	26	recovered	recover	VBN
cord-268139-tgpsu4qz	119	27	at	at	IN
cord-268139-tgpsu4qz	119	28	either	either	DT
cord-268139-tgpsu4qz	119	29	37	37	CD
cord-268139-tgpsu4qz	119	30	-C	-C	NNS
cord-268139-tgpsu4qz	119	31	or	or	CC
cord-268139-tgpsu4qz	119	32	32	32	CD
cord-268139-tgpsu4qz	119	33	-C.	-c.	NN
cord-268139-tgpsu4qz	120	1	The	the	DT
cord-268139-tgpsu4qz	120	2	nsp1DFL	nsp1DFL	NNP
cord-268139-tgpsu4qz	120	3	virus	virus	NN
cord-268139-tgpsu4qz	120	4	was	be	VBD
cord-268139-tgpsu4qz	120	5	not	not	RB
cord-268139-tgpsu4qz	120	6	tested	test	VBN
cord-268139-tgpsu4qz	120	7	at	at	IN
cord-268139-tgpsu4qz	120	8	32	32	CD
cord-268139-tgpsu4qz	120	9	-C.	-c.	NN
cord-268139-tgpsu4qz	121	1	Experiments	experiment	NNS
cord-268139-tgpsu4qz	121	2	were	be	VBD
cord-268139-tgpsu4qz	121	3	next	next	RB
cord-268139-tgpsu4qz	121	4	performed	perform	VBN
cord-268139-tgpsu4qz	121	5	to	to	TO
cord-268139-tgpsu4qz	121	6	determine	determine	VB
cord-268139-tgpsu4qz	121	7	if	if	IN
cord-268139-tgpsu4qz	121	8	viral	viral	JJ
cord-268139-tgpsu4qz	121	9	gene	gene	NN
cord-268139-tgpsu4qz	121	10	expression	expression	NN
cord-268139-tgpsu4qz	121	11	occurred	occur	VBD
cord-268139-tgpsu4qz	121	12	in	in	IN
cord-268139-tgpsu4qz	121	13	cells	cell	NNS
cord-268139-tgpsu4qz	121	14	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	121	15	with	with	IN
cord-268139-tgpsu4qz	121	16	RNA	RNA	NNP
cord-268139-tgpsu4qz	121	17	for	for	IN
cord-268139-tgpsu4qz	121	18	mutants	mutant	NNS
cord-268139-tgpsu4qz	121	19	that	that	WDT
cord-268139-tgpsu4qz	121	20	did	do	VBD
cord-268139-tgpsu4qz	121	21	not	not	RB
cord-268139-tgpsu4qz	121	22	produce	produce	VB
cord-268139-tgpsu4qz	121	23	infectious	infectious	JJ
cord-268139-tgpsu4qz	121	24	virus	virus	NN
cord-268139-tgpsu4qz	121	25	(	(	-LRB-
cord-268139-tgpsu4qz	121	26	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	121	27	,	,	,
cord-268139-tgpsu4qz	121	28	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	121	29	,	,	,
cord-268139-tgpsu4qz	121	30	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	121	31	,	,	,
cord-268139-tgpsu4qz	121	32	and	and	CC
cord-268139-tgpsu4qz	121	33	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	121	34	)	)	-RRB-
cord-268139-tgpsu4qz	121	35	.	.	.
cord-268139-tgpsu4qz	122	1	Using	use	VBG
cord-268139-tgpsu4qz	122	2	antisera	antisera	NN
cord-268139-tgpsu4qz	122	3	against	against	IN
cord-268139-tgpsu4qz	122	4	MHV	MHV	NNP
cord-268139-tgpsu4qz	122	5	virions	virion	NNS
cord-268139-tgpsu4qz	122	6	(	(	-LRB-
cord-268139-tgpsu4qz	122	7	a	a	DT
cord-268139-tgpsu4qz	122	8	-	-	HYPH
cord-268139-tgpsu4qz	122	9	MHV	mhv	NN
cord-268139-tgpsu4qz	122	10	)	)	-RRB-
cord-268139-tgpsu4qz	122	11	or	or	CC
cord-268139-tgpsu4qz	122	12	against	against	IN
cord-268139-tgpsu4qz	122	13	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	122	14	(	(	-LRB-
cord-268139-tgpsu4qz	122	15	a	a	DT
cord-268139-tgpsu4qz	122	16	-	-	HYPH
cord-268139-tgpsu4qz	122	17	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	122	18	)	)	-RRB-
cord-268139-tgpsu4qz	122	19	,	,	,
cord-268139-tgpsu4qz	122	20	no	no	DT
cord-268139-tgpsu4qz	122	21	viral	viral	JJ
cord-268139-tgpsu4qz	122	22	proteins	protein	NNS
cord-268139-tgpsu4qz	122	23	could	could	MD
cord-268139-tgpsu4qz	122	24	be	be	VB
cord-268139-tgpsu4qz	122	25	detected	detect	VBN
cord-268139-tgpsu4qz	122	26	by	by	IN
cord-268139-tgpsu4qz	122	27	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	122	28	or	or	CC
cord-268139-tgpsu4qz	122	29	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	122	30	(	(	-LRB-
cord-268139-tgpsu4qz	122	31	data	datum	NNS
cord-268139-tgpsu4qz	122	32	not	not	RB
cord-268139-tgpsu4qz	122	33	shown	show	VBN
cord-268139-tgpsu4qz	122	34	)	)	-RRB-
cord-268139-tgpsu4qz	122	35	.	.	.
cord-268139-tgpsu4qz	123	1	Furthermore	furthermore	RB
cord-268139-tgpsu4qz	123	2	,	,	,
cord-268139-tgpsu4qz	123	3	no	no	DT
cord-268139-tgpsu4qz	123	4	specific	specific	JJ
cord-268139-tgpsu4qz	123	5	products	product	NNS
cord-268139-tgpsu4qz	123	6	were	be	VBD
cord-268139-tgpsu4qz	123	7	amplified	amplify	VBN
cord-268139-tgpsu4qz	123	8	from	from	IN
cord-268139-tgpsu4qz	123	9	electroporated	electroporated	JJ
cord-268139-tgpsu4qz	123	10	cells	cell	NNS
cord-268139-tgpsu4qz	123	11	using	use	VBG
cord-268139-tgpsu4qz	123	12	RT	RT	NNP
cord-268139-tgpsu4qz	123	13	-	-	HYPH
cord-268139-tgpsu4qz	123	14	PCR	PCR	NNP
cord-268139-tgpsu4qz	123	15	and	and	CC
cord-268139-tgpsu4qz	123	16	a	a	DT
cord-268139-tgpsu4qz	123	17	primer	primer	NN
cord-268139-tgpsu4qz	123	18	that	that	WDT
cord-268139-tgpsu4qz	123	19	detects	detect	VBZ
cord-268139-tgpsu4qz	123	20	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	123	21	RNAs	RNAs	NNPS
cord-268139-tgpsu4qz	123	22	(	(	-LRB-
cord-268139-tgpsu4qz	123	23	data	datum	NNS
cord-268139-tgpsu4qz	123	24	not	not	RB
cord-268139-tgpsu4qz	123	25	shown	show	VBN
cord-268139-tgpsu4qz	123	26	)	)	-RRB-
cord-268139-tgpsu4qz	123	27	.	.	.
cord-268139-tgpsu4qz	124	1	These	these	DT
cord-268139-tgpsu4qz	124	2	results	result	NNS
cord-268139-tgpsu4qz	124	3	suggest	suggest	VBP
cord-268139-tgpsu4qz	124	4	that	that	IN
cord-268139-tgpsu4qz	124	5	,	,	,
cord-268139-tgpsu4qz	124	6	if	if	IN
cord-268139-tgpsu4qz	124	7	viral	viral	JJ
cord-268139-tgpsu4qz	124	8	protein	protein	NN
cord-268139-tgpsu4qz	124	9	or	or	CC
cord-268139-tgpsu4qz	124	10	RNA	RNA	NNP
cord-268139-tgpsu4qz	124	11	expression	expression	NN
cord-268139-tgpsu4qz	124	12	occurred	occur	VBD
cord-268139-tgpsu4qz	124	13	,	,	,
cord-268139-tgpsu4qz	124	14	it	-PRON-	PRP
cord-268139-tgpsu4qz	124	15	was	be	VBD
cord-268139-tgpsu4qz	124	16	below	below	IN
cord-268139-tgpsu4qz	124	17	the	the	DT
cord-268139-tgpsu4qz	124	18	level	level	NN
cord-268139-tgpsu4qz	124	19	of	of	IN
cord-268139-tgpsu4qz	124	20	detection	detection	NN
cord-268139-tgpsu4qz	124	21	using	use	VBG
cord-268139-tgpsu4qz	124	22	these	these	DT
cord-268139-tgpsu4qz	124	23	assays	assay	NNS
cord-268139-tgpsu4qz	124	24	.	.	.
cord-268139-tgpsu4qz	125	1	To	to	TO
cord-268139-tgpsu4qz	125	2	determine	determine	VB
cord-268139-tgpsu4qz	125	3	whether	whether	IN
cord-268139-tgpsu4qz	125	4	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	125	5	of	of	IN
cord-268139-tgpsu4qz	125	6	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	125	7	results	result	NNS
cord-268139-tgpsu4qz	125	8	in	in	IN
cord-268139-tgpsu4qz	125	9	viral	viral	JJ
cord-268139-tgpsu4qz	125	10	replication	replication	NN
cord-268139-tgpsu4qz	125	11	defects	defect	NNS
cord-268139-tgpsu4qz	125	12	,	,	,
cord-268139-tgpsu4qz	125	13	single	single	JJ
cord-268139-tgpsu4qz	125	14	-	-	HYPH
cord-268139-tgpsu4qz	125	15	cycle	cycle	NN
cord-268139-tgpsu4qz	125	16	replication	replication	NN
cord-268139-tgpsu4qz	125	17	assays	assay	NNS
cord-268139-tgpsu4qz	125	18	were	be	VBD
cord-268139-tgpsu4qz	125	19	performed	perform	VBN
cord-268139-tgpsu4qz	125	20	with	with	IN
cord-268139-tgpsu4qz	125	21	the	the	DT
cord-268139-tgpsu4qz	125	22	nsp1	nsp1	CD
cord-268139-tgpsu4qz	125	23	mutants	mutant	NNS
cord-268139-tgpsu4qz	125	24	(	(	-LRB-
cord-268139-tgpsu4qz	125	25	Fig	fig	NN
cord-268139-tgpsu4qz	125	26	.	.	NNP
cord-268139-tgpsu4qz	125	27	2	2	CD
cord-268139-tgpsu4qz	125	28	)	)	-RRB-
cord-268139-tgpsu4qz	125	29	.	.	.
cord-268139-tgpsu4qz	126	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	126	2	cells	cell	NNS
cord-268139-tgpsu4qz	126	3	were	be	VBD
cord-268139-tgpsu4qz	126	4	infected	infect	VBN
cord-268139-tgpsu4qz	126	5	with	with	IN
cord-268139-tgpsu4qz	126	6	icwt	icwt	NNP
cord-268139-tgpsu4qz	126	7	or	or	CC
cord-268139-tgpsu4qz	126	8	nsp1	nsp1	CD
cord-268139-tgpsu4qz	126	9	mutants	mutant	NNS
cord-268139-tgpsu4qz	126	10	(	(	-LRB-
cord-268139-tgpsu4qz	126	11	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	126	12	,	,	,
cord-268139-tgpsu4qz	126	13	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	126	14	,	,	,
cord-268139-tgpsu4qz	126	15	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	126	16	,	,	,
cord-268139-tgpsu4qz	126	17	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	126	18	,	,	,
cord-268139-tgpsu4qz	126	19	VUSB7	VUSB7	NNP
cord-268139-tgpsu4qz	126	20	,	,	,
cord-268139-tgpsu4qz	126	21	or	or	CC
cord-268139-tgpsu4qz	126	22	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	126	23	)	)	-RRB-
cord-268139-tgpsu4qz	126	24	.	.	.
cord-268139-tgpsu4qz	127	1	Samples	sample	NNS
cord-268139-tgpsu4qz	127	2	of	of	IN
cord-268139-tgpsu4qz	127	3	infected	infected	JJ
cord-268139-tgpsu4qz	127	4	cell	cell	NN
cord-268139-tgpsu4qz	127	5	media	medium	NNS
cord-268139-tgpsu4qz	127	6	supernatant	supernatant	VBN
cord-268139-tgpsu4qz	127	7	were	be	VBD
cord-268139-tgpsu4qz	127	8	taken	take	VBN
cord-268139-tgpsu4qz	127	9	at	at	IN
cord-268139-tgpsu4qz	127	10	various	various	JJ
cord-268139-tgpsu4qz	127	11	times	time	NNS
cord-268139-tgpsu4qz	127	12	from	from	IN
cord-268139-tgpsu4qz	127	13	1	1	CD
cord-268139-tgpsu4qz	128	1	-24	-24	:
cord-268139-tgpsu4qz	128	2	h	h	FW
cord-268139-tgpsu4qz	128	3	post	post	NN
cord-268139-tgpsu4qz	128	4	-	-	NN
cord-268139-tgpsu4qz	128	5	infection	infection	JJ
cord-268139-tgpsu4qz	128	6	(	(	-LRB-
cord-268139-tgpsu4qz	128	7	p.i	p.i	NNP
cord-268139-tgpsu4qz	128	8	.	.	NNP
cord-268139-tgpsu4qz	128	9	)	)	-RRB-
cord-268139-tgpsu4qz	128	10	,	,	,
cord-268139-tgpsu4qz	128	11	and	and	CC
cord-268139-tgpsu4qz	128	12	viral	viral	JJ
cord-268139-tgpsu4qz	128	13	titers	titer	NNS
cord-268139-tgpsu4qz	128	14	in	in	IN
cord-268139-tgpsu4qz	128	15	each	each	DT
cord-268139-tgpsu4qz	128	16	sample	sample	NN
cord-268139-tgpsu4qz	128	17	were	be	VBD
cord-268139-tgpsu4qz	128	18	determined	determine	VBN
cord-268139-tgpsu4qz	128	19	by	by	IN
cord-268139-tgpsu4qz	128	20	plaque	plaque	NN
cord-268139-tgpsu4qz	128	21	assays	assay	NNS
cord-268139-tgpsu4qz	128	22	.	.	.
cord-268139-tgpsu4qz	129	1	Wild	wild	JJ
cord-268139-tgpsu4qz	129	2	-	-	HYPH
cord-268139-tgpsu4qz	129	3	type	type	NN
cord-268139-tgpsu4qz	129	4	(	(	-LRB-
cord-268139-tgpsu4qz	129	5	icwt	icwt	NNP
cord-268139-tgpsu4qz	129	6	)	)	-RRB-
cord-268139-tgpsu4qz	129	7	or	or	CC
cord-268139-tgpsu4qz	129	8	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	129	9	mutant	mutant	JJ
cord-268139-tgpsu4qz	129	10	viruses	virus	NNS
cord-268139-tgpsu4qz	129	11	were	be	VBD
cord-268139-tgpsu4qz	129	12	used	use	VBN
cord-268139-tgpsu4qz	129	13	to	to	TO
cord-268139-tgpsu4qz	129	14	infect	infect	VB
cord-268139-tgpsu4qz	129	15	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	129	16	cells	cell	NNS
cord-268139-tgpsu4qz	129	17	at	at	IN
cord-268139-tgpsu4qz	129	18	an	an	DT
cord-268139-tgpsu4qz	129	19	MOI	MOI	NNP
cord-268139-tgpsu4qz	129	20	of	of	IN
cord-268139-tgpsu4qz	129	21	5	5	CD
cord-268139-tgpsu4qz	129	22	.	.	.
cord-268139-tgpsu4qz	130	1	Cells	cell	NNS
cord-268139-tgpsu4qz	130	2	were	be	VBD
cord-268139-tgpsu4qz	130	3	rinsed	rinse	VBN
cord-268139-tgpsu4qz	130	4	three	three	CD
cord-268139-tgpsu4qz	130	5	times	time	NNS
cord-268139-tgpsu4qz	130	6	with	with	IN
cord-268139-tgpsu4qz	130	7	PBS	PBS	NNP
cord-268139-tgpsu4qz	130	8	,	,	,
cord-268139-tgpsu4qz	130	9	incubated	incubate	VBN
cord-268139-tgpsu4qz	130	10	under	under	IN
cord-268139-tgpsu4qz	130	11	medium	medium	NN
cord-268139-tgpsu4qz	130	12	at	at	IN
cord-268139-tgpsu4qz	130	13	37	37	CD
cord-268139-tgpsu4qz	130	14	-C	-C	NNS
cord-268139-tgpsu4qz	130	15	,	,	,
cord-268139-tgpsu4qz	130	16	and	and	CC
cord-268139-tgpsu4qz	130	17	samples	sample	NNS
cord-268139-tgpsu4qz	130	18	of	of	IN
cord-268139-tgpsu4qz	130	19	medium	medium	NN
cord-268139-tgpsu4qz	130	20	were	be	VBD
cord-268139-tgpsu4qz	130	21	obtained	obtain	VBN
cord-268139-tgpsu4qz	130	22	at	at	IN
cord-268139-tgpsu4qz	130	23	the	the	DT
cord-268139-tgpsu4qz	130	24	indicated	indicate	VBN
cord-268139-tgpsu4qz	130	25	times	time	NNS
cord-268139-tgpsu4qz	130	26	post	post	NN
cord-268139-tgpsu4qz	130	27	-	-	NN
cord-268139-tgpsu4qz	130	28	infection	infection	NN
cord-268139-tgpsu4qz	130	29	.	.	.
cord-268139-tgpsu4qz	131	1	Viral	viral	JJ
cord-268139-tgpsu4qz	131	2	titers	titer	NNS
cord-268139-tgpsu4qz	131	3	in	in	IN
cord-268139-tgpsu4qz	131	4	each	each	DT
cord-268139-tgpsu4qz	131	5	sample	sample	NN
cord-268139-tgpsu4qz	131	6	were	be	VBD
cord-268139-tgpsu4qz	131	7	determined	determine	VBN
cord-268139-tgpsu4qz	131	8	using	use	VBG
cord-268139-tgpsu4qz	131	9	plaque	plaque	NN
cord-268139-tgpsu4qz	131	10	assays	assay	NNS
cord-268139-tgpsu4qz	131	11	on	on	IN
cord-268139-tgpsu4qz	131	12	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	131	13	cells	cell	NNS
cord-268139-tgpsu4qz	131	14	at	at	IN
cord-268139-tgpsu4qz	131	15	37	37	CD
cord-268139-tgpsu4qz	131	16	-C.	-c.	NN
cord-268139-tgpsu4qz	132	1	The	the	DT
cord-268139-tgpsu4qz	132	2	graph	graph	NN
cord-268139-tgpsu4qz	132	3	shows	show	VBZ
cord-268139-tgpsu4qz	132	4	the	the	DT
cord-268139-tgpsu4qz	132	5	results	result	NNS
cord-268139-tgpsu4qz	132	6	of	of	IN
cord-268139-tgpsu4qz	132	7	a	a	DT
cord-268139-tgpsu4qz	132	8	representative	representative	JJ
cord-268139-tgpsu4qz	132	9	experiment	experiment	NN
cord-268139-tgpsu4qz	132	10	.	.	.
cord-268139-tgpsu4qz	133	1	Values	value	NNS
cord-268139-tgpsu4qz	133	2	are	be	VBP
cord-268139-tgpsu4qz	133	3	the	the	DT
cord-268139-tgpsu4qz	133	4	averages	average	NNS
cord-268139-tgpsu4qz	133	5	obtained	obtain	VBN
cord-268139-tgpsu4qz	133	6	from	from	IN
cord-268139-tgpsu4qz	133	7	duplicate	duplicate	JJ
cord-268139-tgpsu4qz	133	8	media	medium	NNS
cord-268139-tgpsu4qz	133	9	samples	sample	NNS
cord-268139-tgpsu4qz	133	10	.	.	.
cord-268139-tgpsu4qz	134	1	(	(	-LRB-
cord-268139-tgpsu4qz	134	2	B	b	NN
cord-268139-tgpsu4qz	134	3	)	)	-RRB-
cord-268139-tgpsu4qz	135	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	135	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	135	3	viral	viral	JJ
cord-268139-tgpsu4qz	135	4	yield	yield	NN
cord-268139-tgpsu4qz	135	5	.	.	.
cord-268139-tgpsu4qz	136	1	To	to	TO
cord-268139-tgpsu4qz	136	2	determine	determine	VB
cord-268139-tgpsu4qz	136	3	viral	viral	JJ
cord-268139-tgpsu4qz	136	4	yield	yield	NN
cord-268139-tgpsu4qz	136	5	,	,	,
cord-268139-tgpsu4qz	136	6	viral	viral	JJ
cord-268139-tgpsu4qz	136	7	titer	titer	NN
cord-268139-tgpsu4qz	136	8	at	at	IN
cord-268139-tgpsu4qz	136	9	1	1	CD
cord-268139-tgpsu4qz	136	10	h	h	NN
cord-268139-tgpsu4qz	136	11	p.i	p.i	NNP
cord-268139-tgpsu4qz	136	12	.	.	NNP
cord-268139-tgpsu4qz	136	13	was	be	VBD
cord-268139-tgpsu4qz	136	14	subtracted	subtract	VBN
cord-268139-tgpsu4qz	136	15	from	from	IN
cord-268139-tgpsu4qz	136	16	peak	peak	NN
cord-268139-tgpsu4qz	136	17	titer	titer	NN
cord-268139-tgpsu4qz	136	18	for	for	IN
cord-268139-tgpsu4qz	136	19	each	each	DT
cord-268139-tgpsu4qz	136	20	virus	virus	NN
cord-268139-tgpsu4qz	136	21	.	.	.
cord-268139-tgpsu4qz	137	1	Bars	bar	NNS
cord-268139-tgpsu4qz	137	2	represent	represent	VBP
cord-268139-tgpsu4qz	137	3	average	average	JJ
cord-268139-tgpsu4qz	137	4	viral	viral	JJ
cord-268139-tgpsu4qz	137	5	yield	yield	NN
cord-268139-tgpsu4qz	137	6	calculated	calculate	VBN
cord-268139-tgpsu4qz	137	7	from	from	IN
cord-268139-tgpsu4qz	137	8	the	the	DT
cord-268139-tgpsu4qz	137	9	experiments	experiment	NNS
cord-268139-tgpsu4qz	137	10	(	(	-LRB-
cord-268139-tgpsu4qz	137	11	n	n	NN
cord-268139-tgpsu4qz	137	12	=	=	SYM
cord-268139-tgpsu4qz	137	13	3	3	CD
cord-268139-tgpsu4qz	137	14	)	)	-RRB-
cord-268139-tgpsu4qz	137	15	.	.	.
cord-268139-tgpsu4qz	138	1	Lines	line	NNS
cord-268139-tgpsu4qz	138	2	represent	represent	VBP
cord-268139-tgpsu4qz	138	3	standard	standard	JJ
cord-268139-tgpsu4qz	138	4	error	error	NN
cord-268139-tgpsu4qz	138	5	from	from	IN
cord-268139-tgpsu4qz	138	6	the	the	DT
cord-268139-tgpsu4qz	138	7	experiments	experiment	NNS
cord-268139-tgpsu4qz	138	8	.	.	.
cord-268139-tgpsu4qz	139	1	Statistical	statistical	JJ
cord-268139-tgpsu4qz	139	2	analysis	analysis	NN
cord-268139-tgpsu4qz	139	3	software	software	NN
cord-268139-tgpsu4qz	139	4	was	be	VBD
cord-268139-tgpsu4qz	139	5	used	use	VBN
cord-268139-tgpsu4qz	139	6	to	to	TO
cord-268139-tgpsu4qz	139	7	determine	determine	VB
cord-268139-tgpsu4qz	139	8	P	p	NN
cord-268139-tgpsu4qz	139	9	values	value	NNS
cord-268139-tgpsu4qz	139	10	using	use	VBG
cord-268139-tgpsu4qz	139	11	a	a	DT
cord-268139-tgpsu4qz	139	12	two	two	CD
cord-268139-tgpsu4qz	139	13	-	-	HYPH
cord-268139-tgpsu4qz	139	14	sample	sample	NN
cord-268139-tgpsu4qz	139	15	t	t	NN
cord-268139-tgpsu4qz	139	16	test	test	NN
cord-268139-tgpsu4qz	139	17	.	.	.
cord-268139-tgpsu4qz	140	1	Asterisks	asterisk	NNS
cord-268139-tgpsu4qz	140	2	(	(	-LRB-
cord-268139-tgpsu4qz	140	3	*	*	NFP
cord-268139-tgpsu4qz	140	4	)	)	-RRB-
cord-268139-tgpsu4qz	140	5	indicate	indicate	VBP
cord-268139-tgpsu4qz	140	6	P	p	NN
cord-268139-tgpsu4qz	140	7	values	value	NNS
cord-268139-tgpsu4qz	140	8	0.05	0.05	CD
cord-268139-tgpsu4qz	140	9	.	.	.
cord-268139-tgpsu4qz	141	1	(	(	-LRB-
cord-268139-tgpsu4qz	141	2	C	C	NNP
cord-268139-tgpsu4qz	141	3	)	)	-RRB-
cord-268139-tgpsu4qz	142	1	Relative	relative	JJ
cord-268139-tgpsu4qz	142	2	plaque	plaque	NN
cord-268139-tgpsu4qz	142	3	size	size	NN
cord-268139-tgpsu4qz	142	4	of	of	IN
cord-268139-tgpsu4qz	142	5	icwt	icwt	NNP
cord-268139-tgpsu4qz	142	6	,	,	,
cord-268139-tgpsu4qz	142	7	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	142	8	,	,	,
cord-268139-tgpsu4qz	142	9	and	and	CC
cord-268139-tgpsu4qz	142	10	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	142	11	(	(	-LRB-
cord-268139-tgpsu4qz	142	12	15	15	CD
cord-268139-tgpsu4qz	142	13	h	h	NN
cord-268139-tgpsu4qz	142	14	p.i	p.i	NNP
cord-268139-tgpsu4qz	142	15	.	.	NNP
cord-268139-tgpsu4qz	142	16	)	)	-RRB-
cord-268139-tgpsu4qz	142	17	.	.	.
cord-268139-tgpsu4qz	143	1	Images	image	NNS
cord-268139-tgpsu4qz	143	2	were	be	VBD
cord-268139-tgpsu4qz	143	3	obtained	obtain	VBN
cord-268139-tgpsu4qz	143	4	at	at	IN
cord-268139-tgpsu4qz	143	5	the	the	DT
cord-268139-tgpsu4qz	143	6	same	same	JJ
cord-268139-tgpsu4qz	143	7	resolution	resolution	NN
cord-268139-tgpsu4qz	143	8	(	(	-LRB-
cord-268139-tgpsu4qz	143	9	10Â	10Â	NNP
cord-268139-tgpsu4qz	143	10	)	)	-RRB-
cord-268139-tgpsu4qz	143	11	on	on	IN
cord-268139-tgpsu4qz	143	12	a	a	DT
cord-268139-tgpsu4qz	143	13	Nikon	Nikon	NNP
cord-268139-tgpsu4qz	143	14	Eclipse	Eclipse	NNP
cord-268139-tgpsu4qz	143	15	TE2000-E	te2000-e	NN
cord-268139-tgpsu4qz	143	16	microscope	microscope	NN
cord-268139-tgpsu4qz	143	17	.	.	.
cord-268139-tgpsu4qz	144	1	White	white	JJ
cord-268139-tgpsu4qz	144	2	circles	circle	NNS
cord-268139-tgpsu4qz	144	3	were	be	VBD
cord-268139-tgpsu4qz	144	4	drawn	draw	VBN
cord-268139-tgpsu4qz	144	5	to	to	TO
cord-268139-tgpsu4qz	144	6	facilitate	facilitate	VB
cord-268139-tgpsu4qz	144	7	visualization	visualization	NN
cord-268139-tgpsu4qz	144	8	of	of	IN
cord-268139-tgpsu4qz	144	9	the	the	DT
cord-268139-tgpsu4qz	144	10	plaque	plaque	NN
cord-268139-tgpsu4qz	144	11	boundary	boundary	NN
cord-268139-tgpsu4qz	144	12	.	.	.
cord-268139-tgpsu4qz	145	1	The	the	DT
cord-268139-tgpsu4qz	145	2	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	145	3	mutants	mutant	NNS
cord-268139-tgpsu4qz	145	4	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	145	5	viral	viral	JJ
cord-268139-tgpsu4qz	145	6	replication	replication	NN
cord-268139-tgpsu4qz	145	7	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	145	8	similar	similar	JJ
cord-268139-tgpsu4qz	145	9	to	to	IN
cord-268139-tgpsu4qz	145	10	icwt	icwt	NNP
cord-268139-tgpsu4qz	145	11	with	with	IN
cord-268139-tgpsu4qz	145	12	peak	peak	NN
cord-268139-tgpsu4qz	145	13	virus	virus	NN
cord-268139-tgpsu4qz	145	14	release	release	NN
cord-268139-tgpsu4qz	145	15	occurring	occur	VBG
cord-268139-tgpsu4qz	145	16	between	between	IN
cord-268139-tgpsu4qz	145	17	12	12	CD
cord-268139-tgpsu4qz	145	18	-16	-16	NN
cord-268139-tgpsu4qz	145	19	h	h	NN
cord-268139-tgpsu4qz	145	20	p.i	p.i	NNP
cord-268139-tgpsu4qz	145	21	.	.	.
cord-268139-tgpsu4qz	145	22	(	(	-LRB-
cord-268139-tgpsu4qz	145	23	Fig	Fig	NNP
cord-268139-tgpsu4qz	145	24	.	.	.
cord-268139-tgpsu4qz	145	25	2A	2a	CD
cord-268139-tgpsu4qz	145	26	)	)	-RRB-
cord-268139-tgpsu4qz	145	27	.	.	.
cord-268139-tgpsu4qz	146	1	However	however	RB
cord-268139-tgpsu4qz	146	2	,	,	,
cord-268139-tgpsu4qz	146	3	several	several	JJ
cord-268139-tgpsu4qz	146	4	of	of	IN
cord-268139-tgpsu4qz	146	5	the	the	DT
cord-268139-tgpsu4qz	146	6	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	146	7	mutants	mutant	NNS
cord-268139-tgpsu4qz	146	8	(	(	-LRB-
cord-268139-tgpsu4qz	146	9	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	146	10	,	,	,
cord-268139-tgpsu4qz	146	11	VUSB	VUSB	NNP
cord-268139-tgpsu4qz	146	12	4	4	CD
cord-268139-tgpsu4qz	146	13	,	,	,
cord-268139-tgpsu4qz	146	14	and	and	CC
cord-268139-tgpsu4qz	146	15	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	146	16	)	)	-RRB-
cord-268139-tgpsu4qz	146	17	showed	show	VBD
cord-268139-tgpsu4qz	146	18	a	a	DT
cord-268139-tgpsu4qz	146	19	reduction	reduction	NN
cord-268139-tgpsu4qz	146	20	in	in	IN
cord-268139-tgpsu4qz	146	21	viral	viral	JJ
cord-268139-tgpsu4qz	146	22	yield	yield	NN
cord-268139-tgpsu4qz	146	23	compared	compare	VBN
cord-268139-tgpsu4qz	146	24	with	with	IN
cord-268139-tgpsu4qz	146	25	icwt	icwt	FW
cord-268139-tgpsu4qz	146	26	(	(	-LRB-
cord-268139-tgpsu4qz	146	27	Fig	Fig	NNP
cord-268139-tgpsu4qz	146	28	.	.	.
cord-268139-tgpsu4qz	146	29	2B	2b	CD
cord-268139-tgpsu4qz	146	30	)	)	-RRB-
cord-268139-tgpsu4qz	146	31	.	.	.
cord-268139-tgpsu4qz	147	1	Whereas	whereas	IN
cord-268139-tgpsu4qz	147	2	icwt	icwt	NNP
cord-268139-tgpsu4qz	147	3	gave	give	VBD
cord-268139-tgpsu4qz	147	4	an	an	DT
cord-268139-tgpsu4qz	147	5	average	average	JJ
cord-268139-tgpsu4qz	147	6	yield	yield	NN
cord-268139-tgpsu4qz	147	7	of	of	IN
cord-268139-tgpsu4qz	147	8	approximately	approximately	RB
cord-268139-tgpsu4qz	147	9	4.9	4.9	CD
cord-268139-tgpsu4qz	147	10	Â	â	CD
cord-268139-tgpsu4qz	147	11	10	10	CD
cord-268139-tgpsu4qz	147	12	6	6	CD
cord-268139-tgpsu4qz	147	13	PFU	pfu	NN
cord-268139-tgpsu4qz	147	14	/	/	SYM
cord-268139-tgpsu4qz	147	15	ml	ml	NN
cord-268139-tgpsu4qz	147	16	,	,	,
cord-268139-tgpsu4qz	147	17	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	147	18	,	,	,
cord-268139-tgpsu4qz	147	19	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	147	20	,	,	,
cord-268139-tgpsu4qz	147	21	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	147	22	viruses	virus	NNS
cord-268139-tgpsu4qz	147	23	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	147	24	viral	viral	JJ
cord-268139-tgpsu4qz	147	25	yields	yield	NNS
cord-268139-tgpsu4qz	147	26	of	of	IN
cord-268139-tgpsu4qz	147	27	2.4	2.4	CD
cord-268139-tgpsu4qz	147	28	Â	â	CD
cord-268139-tgpsu4qz	147	29	10	10	CD
cord-268139-tgpsu4qz	147	30	5	5	CD
cord-268139-tgpsu4qz	147	31	,	,	,
cord-268139-tgpsu4qz	147	32	1.4	1.4	CD
cord-268139-tgpsu4qz	147	33	Â	â	CD
cord-268139-tgpsu4qz	147	34	10	10	CD
cord-268139-tgpsu4qz	147	35	6	6	CD
cord-268139-tgpsu4qz	147	36	,	,	,
cord-268139-tgpsu4qz	147	37	1.0	1.0	CD
cord-268139-tgpsu4qz	147	38	Â	â	CD
cord-268139-tgpsu4qz	147	39	10	10	CD
cord-268139-tgpsu4qz	147	40	6	6	CD
cord-268139-tgpsu4qz	147	41	PFU	pfu	NN
cord-268139-tgpsu4qz	147	42	/	/	SYM
cord-268139-tgpsu4qz	147	43	ml	ml	NNS
cord-268139-tgpsu4qz	147	44	,	,	,
cord-268139-tgpsu4qz	147	45	respectively	respectively	RB
cord-268139-tgpsu4qz	147	46	.	.	.
cord-268139-tgpsu4qz	148	1	All	all	DT
cord-268139-tgpsu4qz	148	2	of	of	IN
cord-268139-tgpsu4qz	148	3	the	the	DT
cord-268139-tgpsu4qz	148	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	148	5	mutants	mutant	NNS
cord-268139-tgpsu4qz	148	6	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	148	7	decreased	decreased	JJ
cord-268139-tgpsu4qz	148	8	yields	yield	NNS
cord-268139-tgpsu4qz	148	9	;	;	:
cord-268139-tgpsu4qz	148	10	however	however	RB
cord-268139-tgpsu4qz	148	11	,	,	,
cord-268139-tgpsu4qz	148	12	only	only	RB
cord-268139-tgpsu4qz	148	13	the	the	DT
cord-268139-tgpsu4qz	148	14	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	148	15	,	,	,
cord-268139-tgpsu4qz	148	16	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	148	17	,	,	,
cord-268139-tgpsu4qz	148	18	and	and	CC
cord-268139-tgpsu4qz	148	19	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	148	20	yields	yield	NNS
cord-268139-tgpsu4qz	148	21	were	be	VBD
cord-268139-tgpsu4qz	148	22	statistically	statistically	RB
cord-268139-tgpsu4qz	148	23	significant	significant	JJ
cord-268139-tgpsu4qz	148	24	(	(	-LRB-
cord-268139-tgpsu4qz	148	25	P	p	NN
cord-268139-tgpsu4qz	148	26	0.05	0.05	CD
cord-268139-tgpsu4qz	148	27	using	use	VBG
cord-268139-tgpsu4qz	148	28	a	a	DT
cord-268139-tgpsu4qz	148	29	twosample	twosample	NNP
cord-268139-tgpsu4qz	148	30	t	t	NNP
cord-268139-tgpsu4qz	148	31	test	test	NN
cord-268139-tgpsu4qz	148	32	)	)	-RRB-
cord-268139-tgpsu4qz	148	33	.	.	.
cord-268139-tgpsu4qz	149	1	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	149	2	and	and	CC
cord-268139-tgpsu4qz	149	3	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	149	4	also	also	RB
cord-268139-tgpsu4qz	149	5	demonstrated	demonstrate	VBD
cord-268139-tgpsu4qz	149	6	small	small	JJ
cord-268139-tgpsu4qz	149	7	plaque	plaque	NN
cord-268139-tgpsu4qz	149	8	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	149	9	(	(	-LRB-
cord-268139-tgpsu4qz	149	10	Fig	Fig	NNP
cord-268139-tgpsu4qz	149	11	.	.	.
cord-268139-tgpsu4qz	149	12	2C	2C	NNP
cord-268139-tgpsu4qz	149	13	)	)	-RRB-
cord-268139-tgpsu4qz	149	14	.	.	.
cord-268139-tgpsu4qz	150	1	These	these	DT
cord-268139-tgpsu4qz	150	2	results	result	NNS
cord-268139-tgpsu4qz	150	3	indicate	indicate	VBP
cord-268139-tgpsu4qz	150	4	that	that	IN
cord-268139-tgpsu4qz	150	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	150	6	mutants	mutant	NNS
cord-268139-tgpsu4qz	150	7	have	have	VBP
cord-268139-tgpsu4qz	150	8	replication	replication	NN
cord-268139-tgpsu4qz	150	9	defects	defect	NNS
cord-268139-tgpsu4qz	150	10	in	in	IN
cord-268139-tgpsu4qz	150	11	cultured	cultured	JJ
cord-268139-tgpsu4qz	150	12	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	150	13	cells	cell	NNS
cord-268139-tgpsu4qz	150	14	.	.	.
cord-268139-tgpsu4qz	151	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	151	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	151	3	viruses	virus	NNS
cord-268139-tgpsu4qz	151	4	were	be	VBD
cord-268139-tgpsu4qz	151	5	evaluated	evaluate	VBN
cord-268139-tgpsu4qz	151	6	to	to	TO
cord-268139-tgpsu4qz	151	7	determine	determine	VB
cord-268139-tgpsu4qz	151	8	whether	whether	IN
cord-268139-tgpsu4qz	151	9	they	-PRON-	PRP
cord-268139-tgpsu4qz	151	10	are	be	VBP
cord-268139-tgpsu4qz	151	11	defective	defective	JJ
cord-268139-tgpsu4qz	151	12	in	in	IN
cord-268139-tgpsu4qz	151	13	the	the	DT
cord-268139-tgpsu4qz	151	14	expression	expression	NN
cord-268139-tgpsu4qz	151	15	or	or	CC
cord-268139-tgpsu4qz	151	16	processing	processing	NN
cord-268139-tgpsu4qz	151	17	of	of	IN
cord-268139-tgpsu4qz	151	18	viral	viral	JJ
cord-268139-tgpsu4qz	151	19	proteins	protein	NNS
cord-268139-tgpsu4qz	152	1	(	(	-LRB-
cord-268139-tgpsu4qz	152	2	Fig	Fig	NNP
cord-268139-tgpsu4qz	152	3	.	.	.
cord-268139-tgpsu4qz	152	4	3	3	CD
cord-268139-tgpsu4qz	152	5	)	)	-RRB-
cord-268139-tgpsu4qz	152	6	.	.	.
cord-268139-tgpsu4qz	153	1	Immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	153	2	experiments	experiment	NNS
cord-268139-tgpsu4qz	153	3	were	be	VBD
cord-268139-tgpsu4qz	153	4	performed	perform	VBN
cord-268139-tgpsu4qz	153	5	to	to	TO
cord-268139-tgpsu4qz	153	6	analyze	analyze	VB
cord-268139-tgpsu4qz	153	7	processing	processing	NN
cord-268139-tgpsu4qz	153	8	of	of	IN
cord-268139-tgpsu4qz	153	9	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	153	10	and	and	CC
cord-268139-tgpsu4qz	153	11	nsp2	nsp2	NN
cord-268139-tgpsu4qz	153	12	or	or	CC
cord-268139-tgpsu4qz	153	13	3CLpro	3clpro	CD
cord-268139-tgpsu4qz	153	14	-	-	HYPH
cord-268139-tgpsu4qz	153	15	mediated	mediate	VBN
cord-268139-tgpsu4qz	153	16	processing	processing	NN
cord-268139-tgpsu4qz	153	17	of	of	IN
cord-268139-tgpsu4qz	153	18	nsp8	nsp8	NN
cord-268139-tgpsu4qz	153	19	.	.	.
cord-268139-tgpsu4qz	154	1	To	to	TO
cord-268139-tgpsu4qz	154	2	confirm	confirm	VB
cord-268139-tgpsu4qz	154	3	expression	expression	NN
cord-268139-tgpsu4qz	154	4	of	of	IN
cord-268139-tgpsu4qz	154	5	the	the	DT
cord-268139-tgpsu4qz	154	6	structural	structural	JJ
cord-268139-tgpsu4qz	154	7	proteins	protein	NNS
cord-268139-tgpsu4qz	154	8	,	,	,
cord-268139-tgpsu4qz	154	9	antisera	antisera	NNP
cord-268139-tgpsu4qz	154	10	raised	raise	VBD
cord-268139-tgpsu4qz	154	11	against	against	IN
cord-268139-tgpsu4qz	154	12	MHV	MHV	NNP
cord-268139-tgpsu4qz	154	13	virions	virion	NNS
cord-268139-tgpsu4qz	154	14	(	(	-LRB-
cord-268139-tgpsu4qz	154	15	a	a	DT
cord-268139-tgpsu4qz	154	16	-	-	HYPH
cord-268139-tgpsu4qz	154	17	MHV	mhv	NN
cord-268139-tgpsu4qz	154	18	)	)	-RRB-
cord-268139-tgpsu4qz	154	19	were	be	VBD
cord-268139-tgpsu4qz	154	20	used	use	VBN
cord-268139-tgpsu4qz	154	21	.	.	.
cord-268139-tgpsu4qz	155	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	155	2	cells	cell	NNS
cord-268139-tgpsu4qz	155	3	were	be	VBD
cord-268139-tgpsu4qz	155	4	either	either	RB
cord-268139-tgpsu4qz	155	5	mockinfected	mockinfected	JJ
cord-268139-tgpsu4qz	155	6	or	or	CC
cord-268139-tgpsu4qz	155	7	infected	infect	VBN
cord-268139-tgpsu4qz	155	8	with	with	IN
cord-268139-tgpsu4qz	155	9	icwt	icwt	NNP
cord-268139-tgpsu4qz	155	10	or	or	CC
cord-268139-tgpsu4qz	155	11	nsp1	nsp1	CD
cord-268139-tgpsu4qz	155	12	mutants	mutant	NNS
cord-268139-tgpsu4qz	155	13	,	,	,
cord-268139-tgpsu4qz	155	14	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	155	15	from	from	IN
cord-268139-tgpsu4qz	155	16	5	5	CD
cord-268139-tgpsu4qz	155	17	-	-	SYM
cord-268139-tgpsu4qz	155	18	8	8	CD
cord-268139-tgpsu4qz	155	19	h	h	NN
cord-268139-tgpsu4qz	155	20	p.i	p.i	NNP
cord-268139-tgpsu4qz	155	21	.	.	NNP
cord-268139-tgpsu4qz	155	22	with	with	IN
cord-268139-tgpsu4qz	155	23	[	[	-LRB-
cord-268139-tgpsu4qz	155	24	35	35	CD
cord-268139-tgpsu4qz	155	25	S]Met	S]Met	NNP
cord-268139-tgpsu4qz	155	26	/	/	SYM
cord-268139-tgpsu4qz	155	27	Cys	Cys	NNP
cord-268139-tgpsu4qz	155	28	,	,	,
cord-268139-tgpsu4qz	155	29	and	and	CC
cord-268139-tgpsu4qz	155	30	used	use	VBD
cord-268139-tgpsu4qz	155	31	to	to	TO
cord-268139-tgpsu4qz	155	32	prepare	prepare	VB
cord-268139-tgpsu4qz	155	33	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	155	34	lysates	lysate	NNS
cord-268139-tgpsu4qz	155	35	for	for	IN
cord-268139-tgpsu4qz	155	36	immunoprecipitations	immunoprecipitation	NNS
cord-268139-tgpsu4qz	155	37	.	.	.
cord-268139-tgpsu4qz	156	1	Antisera	Antisera	NNP
cord-268139-tgpsu4qz	156	2	against	against	IN
cord-268139-tgpsu4qz	156	3	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	156	4	(	(	-LRB-
cord-268139-tgpsu4qz	156	5	a	a	DT
cord-268139-tgpsu4qz	156	6	-	-	HYPH
cord-268139-tgpsu4qz	156	7	nsp1	nsp1	CD
cord-268139-tgpsu4qz	156	8	)	)	-RRB-
cord-268139-tgpsu4qz	156	9	immunoprecipitated	immunoprecipitate	VBD
cord-268139-tgpsu4qz	156	10	a	a	DT
cord-268139-tgpsu4qz	156	11	28-kDa	28-kda	CD
cord-268139-tgpsu4qz	156	12	protein	protein	NN
cord-268139-tgpsu4qz	156	13	(	(	-LRB-
cord-268139-tgpsu4qz	156	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	156	15	)	)	-RRB-
cord-268139-tgpsu4qz	156	16	from	from	IN
cord-268139-tgpsu4qz	156	17	cells	cell	NNS
cord-268139-tgpsu4qz	156	18	infected	infect	VBN
cord-268139-tgpsu4qz	156	19	with	with	IN
cord-268139-tgpsu4qz	156	20	icwt	icwt	NNP
cord-268139-tgpsu4qz	156	21	or	or	CC
cord-268139-tgpsu4qz	156	22	the	the	DT
cord-268139-tgpsu4qz	156	23	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	156	24	point	point	NN
cord-268139-tgpsu4qz	156	25	mutants	mutant	NNS
cord-268139-tgpsu4qz	156	26	(	(	-LRB-
cord-268139-tgpsu4qz	156	27	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	156	28	,	,	,
cord-268139-tgpsu4qz	156	29	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	156	30	,	,	,
cord-268139-tgpsu4qz	156	31	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	156	32	,	,	,
cord-268139-tgpsu4qz	156	33	VUSB4	VUSB4	NNS
cord-268139-tgpsu4qz	156	34	,	,	,
cord-268139-tgpsu4qz	156	35	or	or	CC
cord-268139-tgpsu4qz	156	36	VUSB7	vusb7	NN
cord-268139-tgpsu4qz	156	37	)	)	-RRB-
cord-268139-tgpsu4qz	157	1	(	(	-LRB-
cord-268139-tgpsu4qz	157	2	Fig	Fig	NNP
cord-268139-tgpsu4qz	157	3	.	.	.
cord-268139-tgpsu4qz	157	4	3A	3A	NNP
cord-268139-tgpsu4qz	157	5	)	)	-RRB-
cord-268139-tgpsu4qz	157	6	.	.	.
cord-268139-tgpsu4qz	158	1	Migration	migration	NN
cord-268139-tgpsu4qz	158	2	of	of	IN
cord-268139-tgpsu4qz	158	3	VUSB7-nsp1	VUSB7-nsp1	NNPS
cord-268139-tgpsu4qz	158	4	differed	differ	VBD
cord-268139-tgpsu4qz	158	5	from	from	IN
cord-268139-tgpsu4qz	158	6	icwt	icwt	NNP
cord-268139-tgpsu4qz	158	7	and	and	CC
cord-268139-tgpsu4qz	158	8	all	all	DT
cord-268139-tgpsu4qz	158	9	other	other	JJ
cord-268139-tgpsu4qz	158	10	point	point	NN
cord-268139-tgpsu4qz	158	11	mutants	mutant	NNS
cord-268139-tgpsu4qz	158	12	,	,	,
cord-268139-tgpsu4qz	158	13	suggesting	suggest	VBG
cord-268139-tgpsu4qz	158	14	differences	difference	NNS
cord-268139-tgpsu4qz	158	15	in	in	IN
cord-268139-tgpsu4qz	158	16	protein	protein	NN
cord-268139-tgpsu4qz	158	17	folding	folding	NN
cord-268139-tgpsu4qz	158	18	or	or	CC
cord-268139-tgpsu4qz	158	19	charge	charge	NN
cord-268139-tgpsu4qz	158	20	.	.	.
cord-268139-tgpsu4qz	159	1	No	no	DT
cord-268139-tgpsu4qz	159	2	28-kDa	28-kda	CD
cord-268139-tgpsu4qz	159	3	proteins	protein	NNS
cord-268139-tgpsu4qz	159	4	were	be	VBD
cord-268139-tgpsu4qz	159	5	immunoprecipitated	immunoprecipitate	VBN
cord-268139-tgpsu4qz	159	6	from	from	IN
cord-268139-tgpsu4qz	159	7	mock	mock	JJ
cord-268139-tgpsu4qz	159	8	-	-	HYPH
cord-268139-tgpsu4qz	159	9	infected	infected	JJ
cord-268139-tgpsu4qz	159	10	cell	cell	NN
cord-268139-tgpsu4qz	159	11	lysates	lysate	NNS
cord-268139-tgpsu4qz	159	12	or	or	CC
cord-268139-tgpsu4qz	159	13	from	from	IN
cord-268139-tgpsu4qz	159	14	lysates	lysate	NNS
cord-268139-tgpsu4qz	159	15	generated	generate	VBN
cord-268139-tgpsu4qz	159	16	from	from	IN
cord-268139-tgpsu4qz	159	17	cells	cell	NNS
cord-268139-tgpsu4qz	159	18	infected	infect	VBN
cord-268139-tgpsu4qz	159	19	with	with	IN
cord-268139-tgpsu4qz	159	20	the	the	DT
cord-268139-tgpsu4qz	159	21	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	159	22	virus	virus	NN
cord-268139-tgpsu4qz	159	23	(	(	-LRB-
cord-268139-tgpsu4qz	159	24	Fig	Fig	NNP
cord-268139-tgpsu4qz	159	25	.	.	.
cord-268139-tgpsu4qz	159	26	3A	3A	NNP
cord-268139-tgpsu4qz	159	27	)	)	-RRB-
cord-268139-tgpsu4qz	159	28	.	.	.
cord-268139-tgpsu4qz	160	1	Rather	rather	RB
cord-268139-tgpsu4qz	160	2	,	,	,
cord-268139-tgpsu4qz	160	3	from	from	IN
cord-268139-tgpsu4qz	160	4	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	160	5	-	-	HYPH
cord-268139-tgpsu4qz	160	6	infected	infect	VBN
cord-268139-tgpsu4qz	160	7	cell	cell	NN
cord-268139-tgpsu4qz	160	8	lysate	lysate	NN
cord-268139-tgpsu4qz	160	9	,	,	,
cord-268139-tgpsu4qz	160	10	a	a	DT
cord-268139-tgpsu4qz	160	11	-	-	HYPH
cord-268139-tgpsu4qz	160	12	nsp1	nsp1	NN
cord-268139-tgpsu4qz	160	13	immunoprecipitated	immunoprecipitate	VBD
cord-268139-tgpsu4qz	160	14	a	a	DT
cord-268139-tgpsu4qz	160	15	unique	unique	JJ
cord-268139-tgpsu4qz	160	16	14-kDa	14-kda	CD
cord-268139-tgpsu4qz	160	17	protein	protein	NN
cord-268139-tgpsu4qz	160	18	,	,	,
cord-268139-tgpsu4qz	160	19	the	the	DT
cord-268139-tgpsu4qz	160	20	predicted	predict	VBN
cord-268139-tgpsu4qz	160	21	size	size	NN
cord-268139-tgpsu4qz	160	22	of	of	IN
cord-268139-tgpsu4qz	160	23	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	160	24	,	,	,
cord-268139-tgpsu4qz	160	25	as	as	RB
cord-268139-tgpsu4qz	160	26	well	well	RB
cord-268139-tgpsu4qz	160	27	as	as	IN
cord-268139-tgpsu4qz	160	28	a	a	DT
cord-268139-tgpsu4qz	160	29	previously	previously	RB
cord-268139-tgpsu4qz	160	30	undescribed	undescribed	JJ
cord-268139-tgpsu4qz	160	31	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	160	32	protein	protein	NN
cord-268139-tgpsu4qz	160	33	(	(	-LRB-
cord-268139-tgpsu4qz	160	34	Fig	Fig	NNP
cord-268139-tgpsu4qz	160	35	.	.	.
cord-268139-tgpsu4qz	160	36	3A	3A	NNP
cord-268139-tgpsu4qz	160	37	)	)	-RRB-
cord-268139-tgpsu4qz	160	38	.	.	.
cord-268139-tgpsu4qz	161	1	In	in	IN
cord-268139-tgpsu4qz	161	2	both	both	DT
cord-268139-tgpsu4qz	161	3	VUSB4-and	VUSB4-and	NNP
cord-268139-tgpsu4qz	161	4	nsp1DC	nsp1dc	NN
cord-268139-tgpsu4qz	161	5	-	-	HYPH
cord-268139-tgpsu4qz	161	6	infected	infect	VBN
cord-268139-tgpsu4qz	161	7	cells	cell	NNS
cord-268139-tgpsu4qz	161	8	,	,	,
cord-268139-tgpsu4qz	161	9	several	several	JJ
cord-268139-tgpsu4qz	161	10	additional	additional	JJ
cord-268139-tgpsu4qz	161	11	proteins	protein	NNS
cord-268139-tgpsu4qz	161	12	(	(	-LRB-
cord-268139-tgpsu4qz	161	13	30	30	CD
cord-268139-tgpsu4qz	161	14	to	to	TO
cord-268139-tgpsu4qz	161	15	60	60	CD
cord-268139-tgpsu4qz	161	16	kDa	kDa	NNS
cord-268139-tgpsu4qz	161	17	)	)	-RRB-
cord-268139-tgpsu4qz	161	18	of	of	IN
cord-268139-tgpsu4qz	161	19	unknown	unknown	JJ
cord-268139-tgpsu4qz	161	20	identity	identity	NN
cord-268139-tgpsu4qz	161	21	were	be	VBD
cord-268139-tgpsu4qz	161	22	also	also	RB
cord-268139-tgpsu4qz	161	23	precipitated	precipitate	VBN
cord-268139-tgpsu4qz	161	24	using	use	VBG
cord-268139-tgpsu4qz	161	25	a	a	DT
cord-268139-tgpsu4qz	161	26	-	-	HYPH
cord-268139-tgpsu4qz	161	27	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	161	28	(	(	-LRB-
cord-268139-tgpsu4qz	161	29	Fig	Fig	NNP
cord-268139-tgpsu4qz	161	30	.	.	.
cord-268139-tgpsu4qz	161	31	3A	3A	NNP
cord-268139-tgpsu4qz	161	32	)	)	-RRB-
cord-268139-tgpsu4qz	161	33	.	.	.
cord-268139-tgpsu4qz	162	1	Antisera	Antisera	NNP
cord-268139-tgpsu4qz	162	2	against	against	IN
cord-268139-tgpsu4qz	162	3	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	162	4	(	(	-LRB-
cord-268139-tgpsu4qz	162	5	a	a	LS
cord-268139-tgpsu4qz	162	6	-	-	HYPH
cord-268139-tgpsu4qz	162	7	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	162	8	)	)	-RRB-
cord-268139-tgpsu4qz	162	9	immunoprecipitated	immunoprecipitate	VBD
cord-268139-tgpsu4qz	162	10	a	a	DT
cord-268139-tgpsu4qz	162	11	65-kDa	65-kda	CD
cord-268139-tgpsu4qz	162	12	protein	protein	NN
cord-268139-tgpsu4qz	162	13	(	(	-LRB-
cord-268139-tgpsu4qz	162	14	nsp2	nsp2	NN
cord-268139-tgpsu4qz	162	15	)	)	-RRB-
cord-268139-tgpsu4qz	162	16	from	from	IN
cord-268139-tgpsu4qz	162	17	all	all	DT
cord-268139-tgpsu4qz	162	18	infected	infect	VBN
cord-268139-tgpsu4qz	162	19	cell	cell	NN
cord-268139-tgpsu4qz	162	20	lysates	lysate	NNS
cord-268139-tgpsu4qz	162	21	,	,	,
cord-268139-tgpsu4qz	162	22	but	but	CC
cord-268139-tgpsu4qz	162	23	not	not	RB
cord-268139-tgpsu4qz	162	24	from	from	IN
cord-268139-tgpsu4qz	162	25	mock	mock	JJ
cord-268139-tgpsu4qz	162	26	-	-	HYPH
cord-268139-tgpsu4qz	162	27	infected	infect	VBN
cord-268139-tgpsu4qz	162	28	lysates	lysate	NNS
cord-268139-tgpsu4qz	162	29	(	(	-LRB-
cord-268139-tgpsu4qz	162	30	Fig	Fig	NNP
cord-268139-tgpsu4qz	162	31	.	.	.
cord-268139-tgpsu4qz	162	32	3B	3B	NNP
cord-268139-tgpsu4qz	162	33	)	)	-RRB-
cord-268139-tgpsu4qz	162	34	.	.	.
cord-268139-tgpsu4qz	163	1	Similar	similar	JJ
cord-268139-tgpsu4qz	163	2	to	to	IN
cord-268139-tgpsu4qz	163	3	the	the	DT
cord-268139-tgpsu4qz	163	4	result	result	NN
cord-268139-tgpsu4qz	163	5	using	use	VBG
cord-268139-tgpsu4qz	163	6	a	a	DT
cord-268139-tgpsu4qz	163	7	-	-	HYPH
cord-268139-tgpsu4qz	163	8	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	163	9	,	,	,
cord-268139-tgpsu4qz	163	10	an	an	DT
cord-268139-tgpsu4qz	163	11	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	163	12	protein	protein	NN
cord-268139-tgpsu4qz	163	13	was	be	VBD
cord-268139-tgpsu4qz	163	14	also	also	RB
cord-268139-tgpsu4qz	163	15	detected	detect	VBN
cord-268139-tgpsu4qz	163	16	by	by	IN
cord-268139-tgpsu4qz	163	17	a	a	DT
cord-268139-tgpsu4qz	163	18	-	-	HYPH
cord-268139-tgpsu4qz	163	19	nsp2	nsp2	NN
cord-268139-tgpsu4qz	163	20	in	in	IN
cord-268139-tgpsu4qz	163	21	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	163	22	-	-	HYPH
cord-268139-tgpsu4qz	163	23	infected	infect	VBN
cord-268139-tgpsu4qz	163	24	cells	cell	NNS
cord-268139-tgpsu4qz	163	25	(	(	-LRB-
cord-268139-tgpsu4qz	163	26	Fig	fig	NN
cord-268139-tgpsu4qz	163	27	.	.	.
cord-268139-tgpsu4qz	163	28	3B	3B	NNP
cord-268139-tgpsu4qz	163	29	)	)	-RRB-
cord-268139-tgpsu4qz	163	30	.	.	.
cord-268139-tgpsu4qz	164	1	Antisera	Antisera	NNP
cord-268139-tgpsu4qz	164	2	against	against	IN
cord-268139-tgpsu4qz	164	3	nsp8	nsp8	NN
cord-268139-tgpsu4qz	164	4	(	(	-LRB-
cord-268139-tgpsu4qz	164	5	a	a	DT
cord-268139-tgpsu4qz	164	6	-	-	HYPH
cord-268139-tgpsu4qz	164	7	nsp8	nsp8	NNP
cord-268139-tgpsu4qz	164	8	)	)	-RRB-
cord-268139-tgpsu4qz	164	9	immunoprecipitated	immunoprecipitate	VBD
cord-268139-tgpsu4qz	164	10	a	a	DT
cord-268139-tgpsu4qz	164	11	22-kDa	22-kda	CD
cord-268139-tgpsu4qz	164	12	protein	protein	NN
cord-268139-tgpsu4qz	164	13	(	(	-LRB-
cord-268139-tgpsu4qz	164	14	nsp8	nsp8	NN
cord-268139-tgpsu4qz	164	15	)	)	-RRB-
cord-268139-tgpsu4qz	164	16	from	from	IN
cord-268139-tgpsu4qz	164	17	all	all	DT
cord-268139-tgpsu4qz	164	18	infected	infect	VBN
cord-268139-tgpsu4qz	164	19	cell	cell	NN
cord-268139-tgpsu4qz	164	20	lysates	lysate	NNS
cord-268139-tgpsu4qz	164	21	,	,	,
cord-268139-tgpsu4qz	164	22	but	but	CC
cord-268139-tgpsu4qz	164	23	not	not	RB
cord-268139-tgpsu4qz	164	24	from	from	IN
cord-268139-tgpsu4qz	164	25	mock	mock	VB
cord-268139-tgpsu4qz	164	26	-	-	HYPH
cord-268139-tgpsu4qz	164	27	infected	infected	JJ
cord-268139-tgpsu4qz	164	28	cell	cell	NN
cord-268139-tgpsu4qz	164	29	lysates	lysate	NNS
cord-268139-tgpsu4qz	164	30	(	(	-LRB-
cord-268139-tgpsu4qz	164	31	Fig	Fig	NNP
cord-268139-tgpsu4qz	164	32	.	.	.
cord-268139-tgpsu4qz	164	33	3C	3C	NNP
cord-268139-tgpsu4qz	164	34	)	)	-RRB-
cord-268139-tgpsu4qz	164	35	.	.	.
cord-268139-tgpsu4qz	165	1	The	the	DT
cord-268139-tgpsu4qz	165	2	viral	viral	JJ
cord-268139-tgpsu4qz	165	3	structural	structural	JJ
cord-268139-tgpsu4qz	165	4	protein	protein	NN
cord-268139-tgpsu4qz	165	5	S	S	NNP
cord-268139-tgpsu4qz	165	6	,	,	,
cord-268139-tgpsu4qz	165	7	N	N	NNP
cord-268139-tgpsu4qz	165	8	,	,	,
cord-268139-tgpsu4qz	165	9	and	and	CC
cord-268139-tgpsu4qz	165	10	M	M	NNP
cord-268139-tgpsu4qz	165	11	were	be	VBD
cord-268139-tgpsu4qz	165	12	detected	detect	VBN
cord-268139-tgpsu4qz	165	13	in	in	IN
cord-268139-tgpsu4qz	165	14	all	all	DT
cord-268139-tgpsu4qz	165	15	infected	infect	VBN
cord-268139-tgpsu4qz	165	16	cell	cell	NN
cord-268139-tgpsu4qz	165	17	lysates	lysate	NNS
cord-268139-tgpsu4qz	165	18	using	use	VBG
cord-268139-tgpsu4qz	165	19	antisera	antisera	NN
cord-268139-tgpsu4qz	165	20	against	against	IN
cord-268139-tgpsu4qz	165	21	virions	virion	NNS
cord-268139-tgpsu4qz	165	22	(	(	-LRB-
cord-268139-tgpsu4qz	165	23	a	a	DT
cord-268139-tgpsu4qz	165	24	-	-	HYPH
cord-268139-tgpsu4qz	165	25	MHV	mhv	NN
cord-268139-tgpsu4qz	165	26	)	)	-RRB-
cord-268139-tgpsu4qz	165	27	(	(	-LRB-
cord-268139-tgpsu4qz	165	28	Fig	Fig	NNP
cord-268139-tgpsu4qz	165	29	.	.	.
cord-268139-tgpsu4qz	165	30	3D	3D	NNP
cord-268139-tgpsu4qz	165	31	)	)	-RRB-
cord-268139-tgpsu4qz	165	32	.	.	.
cord-268139-tgpsu4qz	166	1	The	the	DT
cord-268139-tgpsu4qz	166	2	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	166	3	mutants	mutant	NNS
cord-268139-tgpsu4qz	166	4	showed	show	VBD
cord-268139-tgpsu4qz	166	5	band	band	NN
cord-268139-tgpsu4qz	166	6	intensities	intensity	NNS
cord-268139-tgpsu4qz	166	7	slightly	slightly	RB
cord-268139-tgpsu4qz	166	8	increased	increase	VBD
cord-268139-tgpsu4qz	166	9	compared	compare	VBN
cord-268139-tgpsu4qz	166	10	with	with	IN
cord-268139-tgpsu4qz	166	11	icwt	icwt	NNP
cord-268139-tgpsu4qz	166	12	.	.	.
cord-268139-tgpsu4qz	167	1	The	the	DT
cord-268139-tgpsu4qz	167	2	reason	reason	NN
cord-268139-tgpsu4qz	167	3	for	for	IN
cord-268139-tgpsu4qz	167	4	this	this	DT
cord-268139-tgpsu4qz	167	5	difference	difference	NN
cord-268139-tgpsu4qz	167	6	in	in	IN
cord-268139-tgpsu4qz	167	7	band	band	NN
cord-268139-tgpsu4qz	167	8	intensity	intensity	NN
cord-268139-tgpsu4qz	167	9	is	be	VBZ
cord-268139-tgpsu4qz	167	10	unknown	unknown	JJ
cord-268139-tgpsu4qz	167	11	but	but	CC
cord-268139-tgpsu4qz	167	12	might	may	MD
cord-268139-tgpsu4qz	167	13	be	be	VB
cord-268139-tgpsu4qz	167	14	a	a	DT
cord-268139-tgpsu4qz	167	15	reflection	reflection	NN
cord-268139-tgpsu4qz	167	16	of	of	IN
cord-268139-tgpsu4qz	167	17	more	more	JJR
cord-268139-tgpsu4qz	167	18	protein	protein	NN
cord-268139-tgpsu4qz	167	19	synthesis	synthesis	NN
cord-268139-tgpsu4qz	167	20	or	or	CC
cord-268139-tgpsu4qz	167	21	processing	processing	NN
cord-268139-tgpsu4qz	167	22	with	with	IN
cord-268139-tgpsu4qz	167	23	the	the	DT
cord-268139-tgpsu4qz	167	24	mutants	mutant	NNS
cord-268139-tgpsu4qz	167	25	at	at	IN
cord-268139-tgpsu4qz	167	26	the	the	DT
cord-268139-tgpsu4qz	167	27	time	time	NN
cord-268139-tgpsu4qz	167	28	of	of	IN
cord-268139-tgpsu4qz	167	29	label	label	NN
cord-268139-tgpsu4qz	167	30	.	.	.
cord-268139-tgpsu4qz	168	1	Still	still	RB
cord-268139-tgpsu4qz	168	2	,	,	,
cord-268139-tgpsu4qz	168	3	although	although	IN
cord-268139-tgpsu4qz	168	4	the	the	DT
cord-268139-tgpsu4qz	168	5	amount	amount	NN
cord-268139-tgpsu4qz	168	6	of	of	IN
cord-268139-tgpsu4qz	168	7	protein	protein	NN
cord-268139-tgpsu4qz	168	8	detected	detect	VBN
cord-268139-tgpsu4qz	168	9	differed	differ	VBD
cord-268139-tgpsu4qz	168	10	slightly	slightly	RB
cord-268139-tgpsu4qz	168	11	,	,	,
cord-268139-tgpsu4qz	168	12	the	the	DT
cord-268139-tgpsu4qz	168	13	results	result	NNS
cord-268139-tgpsu4qz	168	14	show	show	VBP
cord-268139-tgpsu4qz	168	15	that	that	IN
cord-268139-tgpsu4qz	168	16	amino	amino	NN
cord-268139-tgpsu4qz	168	17	-	-	HYPH
cord-268139-tgpsu4qz	168	18	terminal	terminal	NN
cord-268139-tgpsu4qz	168	19	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	168	20	point	point	NN
cord-268139-tgpsu4qz	168	21	mutants	mutant	NNS
cord-268139-tgpsu4qz	168	22	do	do	VBP
cord-268139-tgpsu4qz	168	23	not	not	RB
cord-268139-tgpsu4qz	168	24	have	have	VB
cord-268139-tgpsu4qz	168	25	defects	defect	NNS
cord-268139-tgpsu4qz	168	26	in	in	IN
cord-268139-tgpsu4qz	168	27	their	-PRON-	PRP$
cord-268139-tgpsu4qz	168	28	capacities	capacity	NNS
cord-268139-tgpsu4qz	168	29	to	to	TO
cord-268139-tgpsu4qz	168	30	express	express	VB
cord-268139-tgpsu4qz	168	31	or	or	CC
cord-268139-tgpsu4qz	168	32	process	process	VB
cord-268139-tgpsu4qz	168	33	viral	viral	JJ
cord-268139-tgpsu4qz	168	34	proteins	protein	NNS
cord-268139-tgpsu4qz	168	35	.	.	.
cord-268139-tgpsu4qz	169	1	Nonetheless	nonetheless	RB
cord-268139-tgpsu4qz	169	2	,	,	,
cord-268139-tgpsu4qz	169	3	the	the	DT
cord-268139-tgpsu4qz	169	4	detection	detection	NN
cord-268139-tgpsu4qz	169	5	of	of	IN
cord-268139-tgpsu4qz	169	6	an	an	DT
cord-268139-tgpsu4qz	169	7	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	169	8	protein	protein	NN
cord-268139-tgpsu4qz	169	9	from	from	IN
cord-268139-tgpsu4qz	169	10	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	169	11	-	-	HYPH
cord-268139-tgpsu4qz	169	12	infected	infect	VBN
cord-268139-tgpsu4qz	169	13	cells	cell	NNS
cord-268139-tgpsu4qz	169	14	using	use	VBG
cord-268139-tgpsu4qz	169	15	either	either	CC
cord-268139-tgpsu4qz	169	16	a	a	DT
cord-268139-tgpsu4qz	169	17	-	-	HYPH
cord-268139-tgpsu4qz	169	18	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	169	19	or	or	CC
cord-268139-tgpsu4qz	169	20	a	a	DT
cord-268139-tgpsu4qz	169	21	-	-	HYPH
cord-268139-tgpsu4qz	169	22	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	169	23	suggests	suggest	VBZ
cord-268139-tgpsu4qz	169	24	that	that	IN
cord-268139-tgpsu4qz	169	25	cleavage	cleavage	NN
cord-268139-tgpsu4qz	169	26	of	of	IN
cord-268139-tgpsu4qz	169	27	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	169	28	from	from	IN
cord-268139-tgpsu4qz	169	29	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	169	30	may	may	MD
cord-268139-tgpsu4qz	169	31	be	be	VB
cord-268139-tgpsu4qz	169	32	inefficient	inefficient	JJ
cord-268139-tgpsu4qz	169	33	.	.	.
cord-268139-tgpsu4qz	170	1	Fig	Fig	NNP
cord-268139-tgpsu4qz	170	2	.	.	NNP
cord-268139-tgpsu4qz	170	3	3	3	CD
cord-268139-tgpsu4qz	170	4	.	.	.
cord-268139-tgpsu4qz	171	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	171	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	171	3	viral	viral	JJ
cord-268139-tgpsu4qz	171	4	protein	protein	NN
cord-268139-tgpsu4qz	171	5	expression	expression	NN
cord-268139-tgpsu4qz	171	6	and	and	CC
cord-268139-tgpsu4qz	171	7	processing	processing	NN
cord-268139-tgpsu4qz	171	8	.	.	.
cord-268139-tgpsu4qz	172	1	Cytoplasmic	Cytoplasmic	NNP
cord-268139-tgpsu4qz	172	2	lysates	lysate	NNS
cord-268139-tgpsu4qz	172	3	were	be	VBD
cord-268139-tgpsu4qz	172	4	generated	generate	VBN
cord-268139-tgpsu4qz	172	5	from	from	IN
cord-268139-tgpsu4qz	172	6	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	172	7	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	172	8	cells	cell	NNS
cord-268139-tgpsu4qz	172	9	that	that	WDT
cord-268139-tgpsu4qz	172	10	were	be	VBD
cord-268139-tgpsu4qz	172	11	either	either	DT
cord-268139-tgpsu4qz	172	12	mock	mock	NN
cord-268139-tgpsu4qz	172	13	-	-	HYPH
cord-268139-tgpsu4qz	172	14	infected	infected	JJ
cord-268139-tgpsu4qz	172	15	(	(	-LRB-
cord-268139-tgpsu4qz	172	16	m	m	NN
cord-268139-tgpsu4qz	172	17	)	)	-RRB-
cord-268139-tgpsu4qz	172	18	or	or	CC
cord-268139-tgpsu4qz	172	19	infected	infect	VBN
cord-268139-tgpsu4qz	172	20	with	with	IN
cord-268139-tgpsu4qz	172	21	icwt	icwt	NNP
cord-268139-tgpsu4qz	172	22	or	or	CC
cord-268139-tgpsu4qz	172	23	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	172	24	mutant	mutant	JJ
cord-268139-tgpsu4qz	172	25	viruses	virus	NNS
cord-268139-tgpsu4qz	172	26	.	.	.
cord-268139-tgpsu4qz	173	1	Labeled	label	VBN
cord-268139-tgpsu4qz	173	2	proteins	protein	NNS
cord-268139-tgpsu4qz	173	3	were	be	VBD
cord-268139-tgpsu4qz	173	4	immunoprecipitated	immunoprecipitate	VBN
cord-268139-tgpsu4qz	173	5	from	from	IN
cord-268139-tgpsu4qz	173	6	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	173	7	lysates	lysate	NNS
cord-268139-tgpsu4qz	173	8	with	with	IN
cord-268139-tgpsu4qz	173	9	the	the	DT
cord-268139-tgpsu4qz	173	10	indicated	indicate	VBN
cord-268139-tgpsu4qz	173	11	polyclonal	polyclonal	JJ
cord-268139-tgpsu4qz	173	12	antisera	antisera	NN
cord-268139-tgpsu4qz	173	13	.	.	.
cord-268139-tgpsu4qz	174	1	Proteins	protein	NNS
cord-268139-tgpsu4qz	174	2	were	be	VBD
cord-268139-tgpsu4qz	174	3	resolved	resolve	VBN
cord-268139-tgpsu4qz	174	4	by	by	IN
cord-268139-tgpsu4qz	174	5	SDS	SDS	NNP
cord-268139-tgpsu4qz	174	6	-	-	HYPH
cord-268139-tgpsu4qz	174	7	PAGE	PAGE	NNP
cord-268139-tgpsu4qz	174	8	in	in	IN
cord-268139-tgpsu4qz	174	9	5	5	CD
cord-268139-tgpsu4qz	174	10	-18	-18	CD
cord-268139-tgpsu4qz	174	11	%	%	NN
cord-268139-tgpsu4qz	174	12	polyacrylamide	polyacrylamide	NN
cord-268139-tgpsu4qz	174	13	gradient	gradient	NN
cord-268139-tgpsu4qz	174	14	gels	gel	NNS
cord-268139-tgpsu4qz	174	15	and	and	CC
cord-268139-tgpsu4qz	174	16	visualized	visualize	VBD
cord-268139-tgpsu4qz	174	17	following	follow	VBG
cord-268139-tgpsu4qz	174	18	fluorography	fluorography	NN
cord-268139-tgpsu4qz	174	19	.	.	.
cord-268139-tgpsu4qz	175	1	Images	image	NNS
cord-268139-tgpsu4qz	175	2	were	be	VBD
cord-268139-tgpsu4qz	175	3	obtained	obtain	VBN
cord-268139-tgpsu4qz	175	4	following	follow	VBG
cord-268139-tgpsu4qz	175	5	4-day	4-day	JJ
cord-268139-tgpsu4qz	175	6	film	film	NN
cord-268139-tgpsu4qz	175	7	exposure	exposure	NN
cord-268139-tgpsu4qz	175	8	.	.	.
cord-268139-tgpsu4qz	176	1	Bands	band	NNS
cord-268139-tgpsu4qz	176	2	corresponding	correspond	VBG
cord-268139-tgpsu4qz	176	3	to	to	IN
cord-268139-tgpsu4qz	176	4	unique	unique	JJ
cord-268139-tgpsu4qz	176	5	or	or	CC
cord-268139-tgpsu4qz	176	6	predicted	predict	VBN
cord-268139-tgpsu4qz	176	7	proteins	protein	NNS
cord-268139-tgpsu4qz	176	8	are	be	VBP
cord-268139-tgpsu4qz	176	9	indicated	indicate	VBN
cord-268139-tgpsu4qz	176	10	on	on	IN
cord-268139-tgpsu4qz	176	11	the	the	DT
cord-268139-tgpsu4qz	176	12	right	right	NN
cord-268139-tgpsu4qz	176	13	of	of	IN
cord-268139-tgpsu4qz	176	14	the	the	DT
cord-268139-tgpsu4qz	176	15	fluorograms	fluorogram	NNS
cord-268139-tgpsu4qz	176	16	,	,	,
cord-268139-tgpsu4qz	176	17	and	and	CC
cord-268139-tgpsu4qz	176	18	molecular	molecular	JJ
cord-268139-tgpsu4qz	176	19	weight	weight	NN
cord-268139-tgpsu4qz	176	20	standards	standard	NNS
cord-268139-tgpsu4qz	176	21	(	(	-LRB-
cord-268139-tgpsu4qz	176	22	in	in	IN
cord-268139-tgpsu4qz	176	23	kilodaltons	kilodalton	NNS
cord-268139-tgpsu4qz	176	24	)	)	-RRB-
cord-268139-tgpsu4qz	176	25	are	be	VBP
cord-268139-tgpsu4qz	176	26	shown	show	VBN
cord-268139-tgpsu4qz	176	27	on	on	IN
cord-268139-tgpsu4qz	176	28	the	the	DT
cord-268139-tgpsu4qz	176	29	left	left	NN
cord-268139-tgpsu4qz	176	30	.	.	.
cord-268139-tgpsu4qz	177	1	The	the	DT
cord-268139-tgpsu4qz	177	2	MHV	MHV	NNP
cord-268139-tgpsu4qz	177	3	structural	structural	JJ
cord-268139-tgpsu4qz	177	4	proteins	protein	NNS
cord-268139-tgpsu4qz	177	5	are	be	VBP
cord-268139-tgpsu4qz	177	6	designated	designate	VBN
cord-268139-tgpsu4qz	177	7	as	as	IN
cord-268139-tgpsu4qz	177	8	follows	follow	VBZ
cord-268139-tgpsu4qz	177	9	:	:	:
cord-268139-tgpsu4qz	177	10	spike	spike	NN
cord-268139-tgpsu4qz	177	11	(	(	-LRB-
cord-268139-tgpsu4qz	177	12	S	S	NNP
cord-268139-tgpsu4qz	177	13	)	)	-RRB-
cord-268139-tgpsu4qz	177	14	,	,	,
cord-268139-tgpsu4qz	177	15	nucleocapsid	nucleocapsid	NN
cord-268139-tgpsu4qz	177	16	(	(	-LRB-
cord-268139-tgpsu4qz	177	17	N	N	NNP
cord-268139-tgpsu4qz	177	18	)	)	-RRB-
cord-268139-tgpsu4qz	177	19	,	,	,
cord-268139-tgpsu4qz	177	20	and	and	CC
cord-268139-tgpsu4qz	177	21	membrane	membrane	NN
cord-268139-tgpsu4qz	177	22	(	(	-LRB-
cord-268139-tgpsu4qz	177	23	M	M	NNP
cord-268139-tgpsu4qz	177	24	)	)	-RRB-
cord-268139-tgpsu4qz	177	25	.	.	.
cord-268139-tgpsu4qz	178	1	The	the	DT
cord-268139-tgpsu4qz	178	2	antisera	antisera	NN
cord-268139-tgpsu4qz	178	3	used	use	VBD
cord-268139-tgpsu4qz	178	4	for	for	IN
cord-268139-tgpsu4qz	178	5	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	178	6	are	be	VBP
cord-268139-tgpsu4qz	178	7	indicated	indicate	VBN
cord-268139-tgpsu4qz	178	8	:	:	:
cord-268139-tgpsu4qz	178	9	The	the	DT
cord-268139-tgpsu4qz	178	10	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	178	11	-	-	HYPH
cord-268139-tgpsu4qz	178	12	terminal	terminal	NN
cord-268139-tgpsu4qz	178	13	half	half	NN
cord-268139-tgpsu4qz	178	14	of	of	IN
cord-268139-tgpsu4qz	178	15	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	178	16	is	be	VBZ
cord-268139-tgpsu4qz	178	17	required	require	VBN
cord-268139-tgpsu4qz	178	18	for	for	IN
cord-268139-tgpsu4qz	178	19	efficient	efficient	JJ
cord-268139-tgpsu4qz	178	20	cleavage	cleavage	NN
cord-268139-tgpsu4qz	178	21	at	at	IN
cord-268139-tgpsu4qz	178	22	CS1	CS1	NNP
cord-268139-tgpsu4qz	178	23	Previous	Previous	NNP
cord-268139-tgpsu4qz	178	24	studies	study	NNS
cord-268139-tgpsu4qz	178	25	have	have	VBP
cord-268139-tgpsu4qz	178	26	shown	show	VBN
cord-268139-tgpsu4qz	178	27	that	that	IN
cord-268139-tgpsu4qz	178	28	nsp1	nsp1	NN
cord-268139-tgpsu4qz	178	29	is	be	VBZ
cord-268139-tgpsu4qz	178	30	processed	process	VBN
cord-268139-tgpsu4qz	178	31	from	from	IN
cord-268139-tgpsu4qz	178	32	the	the	DT
cord-268139-tgpsu4qz	178	33	gene	gene	NN
cord-268139-tgpsu4qz	178	34	1	1	CD
cord-268139-tgpsu4qz	178	35	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	178	36	very	very	RB
cord-268139-tgpsu4qz	178	37	rapidly	rapidly	RB
cord-268139-tgpsu4qz	178	38	,	,	,
cord-268139-tgpsu4qz	178	39	and	and	CC
cord-268139-tgpsu4qz	178	40	nsp1-containing	nsp1-containe	VBG
cord-268139-tgpsu4qz	178	41	precursors	precursor	NNS
cord-268139-tgpsu4qz	178	42	have	have	VBP
cord-268139-tgpsu4qz	178	43	not	not	RB
cord-268139-tgpsu4qz	178	44	been	be	VBN
cord-268139-tgpsu4qz	178	45	detected	detect	VBN
cord-268139-tgpsu4qz	179	1	(	(	-LRB-
cord-268139-tgpsu4qz	179	2	Denison	Denison	NNP
cord-268139-tgpsu4qz	179	3	et	et	NNP
cord-268139-tgpsu4qz	179	4	al	al	NNP
cord-268139-tgpsu4qz	179	5	.	.	NNP
cord-268139-tgpsu4qz	179	6	,	,	,
cord-268139-tgpsu4qz	179	7	1992	1992	CD
cord-268139-tgpsu4qz	179	8	)	)	-RRB-
cord-268139-tgpsu4qz	179	9	.	.	.
cord-268139-tgpsu4qz	180	1	In	in	IN
cord-268139-tgpsu4qz	180	2	contrast	contrast	NN
cord-268139-tgpsu4qz	180	3	,	,	,
cord-268139-tgpsu4qz	180	4	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	180	5	is	be	VBZ
cord-268139-tgpsu4qz	180	6	processed	process	VBN
cord-268139-tgpsu4qz	180	7	with	with	IN
cord-268139-tgpsu4qz	180	8	slower	slow	JJR
cord-268139-tgpsu4qz	180	9	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	180	10	likely	likely	RB
cord-268139-tgpsu4qz	180	11	from	from	IN
cord-268139-tgpsu4qz	180	12	a	a	DT
cord-268139-tgpsu4qz	180	13	275-kDa	275-kda	CD
cord-268139-tgpsu4qz	180	14	nsp2	nsp2	NN
cord-268139-tgpsu4qz	180	15	-nsp3	-nsp3	NN
cord-268139-tgpsu4qz	180	16	precursor	precursor	NN
cord-268139-tgpsu4qz	180	17	protein	protein	NN
cord-268139-tgpsu4qz	180	18	(	(	-LRB-
cord-268139-tgpsu4qz	180	19	Denison	Denison	NNP
cord-268139-tgpsu4qz	180	20	et	et	NNP
cord-268139-tgpsu4qz	180	21	al	al	NNP
cord-268139-tgpsu4qz	180	22	.	.	NNP
cord-268139-tgpsu4qz	180	23	,	,	,
cord-268139-tgpsu4qz	180	24	1992	1992	CD
cord-268139-tgpsu4qz	181	1	Harcourt	Harcourt	NNP
cord-268139-tgpsu4qz	181	2	et	et	NNP
cord-268139-tgpsu4qz	181	3	al	al	NNP
cord-268139-tgpsu4qz	181	4	.	.	NNP
cord-268139-tgpsu4qz	181	5	,	,	,
cord-268139-tgpsu4qz	181	6	2004	2004	CD
cord-268139-tgpsu4qz	182	1	;	;	:
cord-268139-tgpsu4qz	182	2	Schiller	Schiller	NNP
cord-268139-tgpsu4qz	182	3	et	et	NNP
cord-268139-tgpsu4qz	182	4	al	al	NNP
cord-268139-tgpsu4qz	182	5	.	.	NNP
cord-268139-tgpsu4qz	182	6	,	,	,
cord-268139-tgpsu4qz	182	7	1998	1998	CD
cord-268139-tgpsu4qz	182	8	)	)	-RRB-
cord-268139-tgpsu4qz	182	9	.	.	.
cord-268139-tgpsu4qz	183	1	To	to	TO
cord-268139-tgpsu4qz	183	2	determine	determine	VB
cord-268139-tgpsu4qz	183	3	the	the	DT
cord-268139-tgpsu4qz	183	4	expression	expression	NN
cord-268139-tgpsu4qz	183	5	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	183	6	of	of	IN
cord-268139-tgpsu4qz	183	7	the	the	DT
cord-268139-tgpsu4qz	183	8	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	183	9	protein	protein	NN
cord-268139-tgpsu4qz	183	10	in	in	IN
cord-268139-tgpsu4qz	183	11	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	183	12	virus	virus	NN
cord-268139-tgpsu4qz	183	13	-	-	HYPH
cord-268139-tgpsu4qz	183	14	infected	infect	VBN
cord-268139-tgpsu4qz	183	15	cells	cell	NNS
cord-268139-tgpsu4qz	183	16	and	and	CC
cord-268139-tgpsu4qz	183	17	to	to	TO
cord-268139-tgpsu4qz	183	18	determine	determine	VB
cord-268139-tgpsu4qz	183	19	whether	whether	IN
cord-268139-tgpsu4qz	183	20	this	this	DT
cord-268139-tgpsu4qz	183	21	protein	protein	NN
cord-268139-tgpsu4qz	183	22	is	be	VBZ
cord-268139-tgpsu4qz	183	23	capable	capable	JJ
cord-268139-tgpsu4qz	183	24	of	of	IN
cord-268139-tgpsu4qz	183	25	being	be	VBG
cord-268139-tgpsu4qz	183	26	processed	process	VBN
cord-268139-tgpsu4qz	183	27	into	into	IN
cord-268139-tgpsu4qz	183	28	14-kDa	14-kda	CD
cord-268139-tgpsu4qz	183	29	(	(	-LRB-
cord-268139-tgpsu4qz	183	30	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	183	31	)	)	-RRB-
cord-268139-tgpsu4qz	183	32	and	and	CC
cord-268139-tgpsu4qz	183	33	65-kDa	65-kda	CD
cord-268139-tgpsu4qz	183	34	(	(	-LRB-
cord-268139-tgpsu4qz	183	35	nsp2	nsp2	NN
cord-268139-tgpsu4qz	183	36	)	)	-RRB-
cord-268139-tgpsu4qz	183	37	proteins	protein	NNS
cord-268139-tgpsu4qz	183	38	,	,	,
cord-268139-tgpsu4qz	184	1	pulse	pulse	NN
cord-268139-tgpsu4qz	184	2	-	-	HYPH
cord-268139-tgpsu4qz	184	3	chase	chase	NN
cord-268139-tgpsu4qz	184	4	translation	translation	NN
cord-268139-tgpsu4qz	184	5	experiments	experiment	NNS
cord-268139-tgpsu4qz	184	6	were	be	VBD
cord-268139-tgpsu4qz	184	7	performed	perform	VBN
cord-268139-tgpsu4qz	184	8	(	(	-LRB-
cord-268139-tgpsu4qz	184	9	Fig	Fig	NNP
cord-268139-tgpsu4qz	184	10	.	.	NNP
cord-268139-tgpsu4qz	184	11	4	4	CD
cord-268139-tgpsu4qz	184	12	)	)	-RRB-
cord-268139-tgpsu4qz	184	13	.	.	.
cord-268139-tgpsu4qz	185	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	185	2	cells	cell	NNS
cord-268139-tgpsu4qz	185	3	were	be	VBD
cord-268139-tgpsu4qz	185	4	infected	infect	VBN
cord-268139-tgpsu4qz	185	5	with	with	IN
cord-268139-tgpsu4qz	185	6	either	either	DT
cord-268139-tgpsu4qz	185	7	icwt	icwt	NNP
cord-268139-tgpsu4qz	185	8	or	or	CC
cord-268139-tgpsu4qz	185	9	the	the	DT
cord-268139-tgpsu4qz	185	10	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	185	11	virus	virus	NN
cord-268139-tgpsu4qz	185	12	,	,	,
cord-268139-tgpsu4qz	185	13	and	and	CC
cord-268139-tgpsu4qz	185	14	proteins	protein	NNS
cord-268139-tgpsu4qz	185	15	were	be	VBD
cord-268139-tgpsu4qz	185	16	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	185	17	from	from	IN
cord-268139-tgpsu4qz	185	18	6	6	CD
cord-268139-tgpsu4qz	185	19	-6.5	-6.5	NN
cord-268139-tgpsu4qz	185	20	h	h	NN
cord-268139-tgpsu4qz	185	21	p.i	p.i	NNP
cord-268139-tgpsu4qz	185	22	.	.	.
cord-268139-tgpsu4qz	186	1	with	with	IN
cord-268139-tgpsu4qz	186	2	[	[	-LRB-
cord-268139-tgpsu4qz	186	3	35	35	CD
cord-268139-tgpsu4qz	186	4	S]Met	S]Met	NNP
cord-268139-tgpsu4qz	186	5	/	/	SYM
cord-268139-tgpsu4qz	186	6	Cys	Cys	NNP
cord-268139-tgpsu4qz	186	7	.	.	.
cord-268139-tgpsu4qz	187	1	Infected	infected	JJ
cord-268139-tgpsu4qz	187	2	cells	cell	NNS
cord-268139-tgpsu4qz	187	3	were	be	VBD
cord-268139-tgpsu4qz	187	4	then	then	RB
cord-268139-tgpsu4qz	187	5	chased	chase	VBN
cord-268139-tgpsu4qz	187	6	in	in	IN
cord-268139-tgpsu4qz	187	7	medium	medium	NN
cord-268139-tgpsu4qz	187	8	without	without	IN
cord-268139-tgpsu4qz	187	9	radiolabel	radiolabel	NNP
cord-268139-tgpsu4qz	187	10	but	but	CC
cord-268139-tgpsu4qz	187	11	containing	contain	VBG
cord-268139-tgpsu4qz	187	12	cyclohexamide	cyclohexamide	NN
cord-268139-tgpsu4qz	187	13	to	to	TO
cord-268139-tgpsu4qz	187	14	inhibit	inhibit	VB
cord-268139-tgpsu4qz	187	15	new	new	JJ
cord-268139-tgpsu4qz	187	16	protein	protein	NN
cord-268139-tgpsu4qz	187	17	synthesis	synthesis	NN
cord-268139-tgpsu4qz	187	18	.	.	.
cord-268139-tgpsu4qz	188	1	Cells	cell	NNS
cord-268139-tgpsu4qz	188	2	were	be	VBD
cord-268139-tgpsu4qz	188	3	harvested	harvest	VBN
cord-268139-tgpsu4qz	188	4	at	at	IN
cord-268139-tgpsu4qz	188	5	various	various	JJ
cord-268139-tgpsu4qz	188	6	times	time	NNS
cord-268139-tgpsu4qz	188	7	post	post	JJ
cord-268139-tgpsu4qz	188	8	-	-	JJ
cord-268139-tgpsu4qz	188	9	chase	chase	JJ
cord-268139-tgpsu4qz	188	10	(	(	-LRB-
cord-268139-tgpsu4qz	188	11	p.c	p.c	NNP
cord-268139-tgpsu4qz	188	12	.	.	NNP
cord-268139-tgpsu4qz	188	13	)	)	-RRB-
cord-268139-tgpsu4qz	188	14	,	,	,
cord-268139-tgpsu4qz	188	15	and	and	CC
cord-268139-tgpsu4qz	188	16	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	188	17	lysates	lysate	NNS
cord-268139-tgpsu4qz	188	18	were	be	VBD
cord-268139-tgpsu4qz	188	19	generated	generate	VBN
cord-268139-tgpsu4qz	188	20	for	for	IN
cord-268139-tgpsu4qz	188	21	immunoprecipitations	immunoprecipitation	NNS
cord-268139-tgpsu4qz	188	22	using	use	VBG
cord-268139-tgpsu4qz	188	23	a	a	DT
cord-268139-tgpsu4qz	188	24	-	-	HYPH
cord-268139-tgpsu4qz	188	25	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	188	26	and	and	CC
cord-268139-tgpsu4qz	188	27	a	a	DT
cord-268139-tgpsu4qz	188	28	-	-	HYPH
cord-268139-tgpsu4qz	188	29	nsp2	nsp2	NN
cord-268139-tgpsu4qz	188	30	.	.	.
cord-268139-tgpsu4qz	189	1	In	in	IN
cord-268139-tgpsu4qz	189	2	the	the	DT
cord-268139-tgpsu4qz	189	3	current	current	JJ
cord-268139-tgpsu4qz	189	4	study	study	NN
cord-268139-tgpsu4qz	189	5	,	,	,
cord-268139-tgpsu4qz	189	6	the	the	DT
cord-268139-tgpsu4qz	189	7	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	189	8	of	of	IN
cord-268139-tgpsu4qz	189	9	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	189	10	and	and	CC
cord-268139-tgpsu4qz	189	11	nsp2	nsp2	NN
cord-268139-tgpsu4qz	189	12	expression	expression	NN
cord-268139-tgpsu4qz	189	13	and	and	CC
cord-268139-tgpsu4qz	189	14	processing	processing	NN
cord-268139-tgpsu4qz	189	15	in	in	IN
cord-268139-tgpsu4qz	189	16	icwt	icwt	NNP
cord-268139-tgpsu4qz	189	17	-	-	HYPH
cord-268139-tgpsu4qz	189	18	infected	infect	VBN
cord-268139-tgpsu4qz	189	19	cells	cell	NNS
cord-268139-tgpsu4qz	189	20	were	be	VBD
cord-268139-tgpsu4qz	189	21	similar	similar	JJ
cord-268139-tgpsu4qz	189	22	to	to	IN
cord-268139-tgpsu4qz	189	23	published	publish	VBN
cord-268139-tgpsu4qz	189	24	reports	report	NNS
cord-268139-tgpsu4qz	189	25	(	(	-LRB-
cord-268139-tgpsu4qz	189	26	Fig	Fig	NNP
cord-268139-tgpsu4qz	189	27	.	.	.
cord-268139-tgpsu4qz	189	28	4A	4a	CD
cord-268139-tgpsu4qz	189	29	)	)	-RRB-
cord-268139-tgpsu4qz	189	30	.	.	.
cord-268139-tgpsu4qz	190	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	190	2	was	be	VBD
cord-268139-tgpsu4qz	190	3	detected	detect	VBN
cord-268139-tgpsu4qz	190	4	as	as	IN
cord-268139-tgpsu4qz	190	5	a	a	DT
cord-268139-tgpsu4qz	190	6	mature	mature	JJ
cord-268139-tgpsu4qz	190	7	28-kDa	28-kda	CD
cord-268139-tgpsu4qz	190	8	protein	protein	NN
cord-268139-tgpsu4qz	190	9	at	at	IN
cord-268139-tgpsu4qz	190	10	0	0	CD
cord-268139-tgpsu4qz	190	11	min	min	NNP
cord-268139-tgpsu4qz	190	12	p.c	p.c	NNP
cord-268139-tgpsu4qz	190	13	.	.	NNP
cord-268139-tgpsu4qz	190	14	,	,	,
cord-268139-tgpsu4qz	190	15	suggesting	suggest	VBG
cord-268139-tgpsu4qz	190	16	that	that	IN
cord-268139-tgpsu4qz	190	17	this	this	DT
cord-268139-tgpsu4qz	190	18	protein	protein	NN
cord-268139-tgpsu4qz	190	19	was	be	VBD
cord-268139-tgpsu4qz	190	20	processed	process	VBN
cord-268139-tgpsu4qz	190	21	during	during	IN
cord-268139-tgpsu4qz	190	22	the	the	DT
cord-268139-tgpsu4qz	190	23	30	30	CD
cord-268139-tgpsu4qz	190	24	min	min	NN
cord-268139-tgpsu4qz	190	25	radiolabeling	radiolabeling	NN
cord-268139-tgpsu4qz	190	26	period	period	NN
cord-268139-tgpsu4qz	190	27	.	.	.
cord-268139-tgpsu4qz	191	1	Although	although	IN
cord-268139-tgpsu4qz	191	2	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	191	3	was	be	VBD
cord-268139-tgpsu4qz	191	4	detected	detect	VBN
cord-268139-tgpsu4qz	191	5	as	as	IN
cord-268139-tgpsu4qz	191	6	a	a	DT
cord-268139-tgpsu4qz	191	7	mature	mature	JJ
cord-268139-tgpsu4qz	191	8	65-kDa	65-kda	CD
cord-268139-tgpsu4qz	191	9	protein	protein	NN
cord-268139-tgpsu4qz	191	10	at	at	IN
cord-268139-tgpsu4qz	191	11	15	15	CD
cord-268139-tgpsu4qz	191	12	min	min	NNP
cord-268139-tgpsu4qz	191	13	p.c	p.c	NNP
cord-268139-tgpsu4qz	191	14	.	.	NNP
cord-268139-tgpsu4qz	191	15	,	,	,
cord-268139-tgpsu4qz	191	16	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	191	17	detection	detection	NN
cord-268139-tgpsu4qz	191	18	was	be	VBD
cord-268139-tgpsu4qz	191	19	increased	increase	VBN
cord-268139-tgpsu4qz	191	20	at	at	IN
cord-268139-tgpsu4qz	191	21	60	60	CD
cord-268139-tgpsu4qz	191	22	-	-	SYM
cord-268139-tgpsu4qz	191	23	360	360	CD
cord-268139-tgpsu4qz	191	24	min	min	NN
cord-268139-tgpsu4qz	191	25	p.c	p.c	NN
cord-268139-tgpsu4qz	191	26	.	.	.
cord-268139-tgpsu4qz	192	1	The	the	DT
cord-268139-tgpsu4qz	192	2	result	result	NN
cord-268139-tgpsu4qz	192	3	that	that	IN
cord-268139-tgpsu4qz	192	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	192	5	and	and	CC
cord-268139-tgpsu4qz	192	6	nsp2	nsp2	NN
cord-268139-tgpsu4qz	192	7	remained	remain	VBD
cord-268139-tgpsu4qz	192	8	detectable	detectable	JJ
cord-268139-tgpsu4qz	192	9	even	even	RB
cord-268139-tgpsu4qz	192	10	at	at	IN
cord-268139-tgpsu4qz	192	11	360	360	CD
cord-268139-tgpsu4qz	192	12	min	min	NN
cord-268139-tgpsu4qz	192	13	p.c	p.c	NNP
cord-268139-tgpsu4qz	192	14	.	.	NNP
cord-268139-tgpsu4qz	192	15	suggested	suggest	VBD
cord-268139-tgpsu4qz	192	16	that	that	IN
cord-268139-tgpsu4qz	192	17	these	these	DT
cord-268139-tgpsu4qz	192	18	proteins	protein	NNS
cord-268139-tgpsu4qz	192	19	were	be	VBD
cord-268139-tgpsu4qz	192	20	stable	stable	JJ
cord-268139-tgpsu4qz	192	21	.	.	.
cord-268139-tgpsu4qz	193	1	In	in	IN
cord-268139-tgpsu4qz	193	2	these	these	DT
cord-268139-tgpsu4qz	193	3	experiments	experiment	NNS
cord-268139-tgpsu4qz	193	4	,	,	,
cord-268139-tgpsu4qz	193	5	the	the	DT
cord-268139-tgpsu4qz	193	6	275-kDa	275-kda	CD
cord-268139-tgpsu4qz	193	7	nsp2	nsp2	NN
cord-268139-tgpsu4qz	193	8	-nsp3	-nsp3	NNP
cord-268139-tgpsu4qz	193	9	precursor	precursor	NN
cord-268139-tgpsu4qz	193	10	was	be	VBD
cord-268139-tgpsu4qz	193	11	not	not	RB
cord-268139-tgpsu4qz	193	12	detected	detect	VBN
cord-268139-tgpsu4qz	193	13	by	by	IN
cord-268139-tgpsu4qz	193	14	a	a	DT
cord-268139-tgpsu4qz	193	15	-	-	HYPH
cord-268139-tgpsu4qz	193	16	nsp2	nsp2	NN
cord-268139-tgpsu4qz	193	17	.	.	.
cord-268139-tgpsu4qz	194	1	Like	like	IN
cord-268139-tgpsu4qz	194	2	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	194	3	from	from	IN
cord-268139-tgpsu4qz	194	4	icwt	icwt	NNP
cord-268139-tgpsu4qz	194	5	,	,	,
cord-268139-tgpsu4qz	194	6	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	194	7	(	(	-LRB-
cord-268139-tgpsu4qz	194	8	14	14	CD
cord-268139-tgpsu4qz	194	9	kDa	kDa	NNS
cord-268139-tgpsu4qz	194	10	)	)	-RRB-
cord-268139-tgpsu4qz	194	11	was	be	VBD
cord-268139-tgpsu4qz	194	12	detectable	detectable	JJ
cord-268139-tgpsu4qz	194	13	at	at	IN
cord-268139-tgpsu4qz	194	14	0	0	CD
cord-268139-tgpsu4qz	194	15	min	min	NNP
cord-268139-tgpsu4qz	194	16	p.c	p.c	NNP
cord-268139-tgpsu4qz	194	17	.	.	NNP
cord-268139-tgpsu4qz	194	18	,	,	,
cord-268139-tgpsu4qz	194	19	indicating	indicate	VBG
cord-268139-tgpsu4qz	194	20	that	that	IN
cord-268139-tgpsu4qz	194	21	processing	processing	NN
cord-268139-tgpsu4qz	194	22	at	at	IN
cord-268139-tgpsu4qz	194	23	CS1	CS1	NNP
cord-268139-tgpsu4qz	194	24	occurred	occur	VBD
cord-268139-tgpsu4qz	194	25	within	within	IN
cord-268139-tgpsu4qz	194	26	Fig	Fig	NNP
cord-268139-tgpsu4qz	194	27	.	.	.
cord-268139-tgpsu4qz	195	1	4	4	LS
cord-268139-tgpsu4qz	195	2	.	.	.
cord-268139-tgpsu4qz	196	1	Pulse	pulse	NN
cord-268139-tgpsu4qz	196	2	-	-	HYPH
cord-268139-tgpsu4qz	196	3	chase	chase	NN
cord-268139-tgpsu4qz	196	4	translation	translation	NN
cord-268139-tgpsu4qz	196	5	in	in	IN
cord-268139-tgpsu4qz	196	6	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	196	7	virus	virus	NN
cord-268139-tgpsu4qz	196	8	-	-	HYPH
cord-268139-tgpsu4qz	196	9	infected	infect	VBN
cord-268139-tgpsu4qz	196	10	cells	cell	NNS
cord-268139-tgpsu4qz	196	11	.	.	.
cord-268139-tgpsu4qz	197	1	Proteins	protein	NNS
cord-268139-tgpsu4qz	197	2	in	in	IN
cord-268139-tgpsu4qz	197	3	infected	infected	JJ
cord-268139-tgpsu4qz	197	4	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	197	5	cells	cell	NNS
cord-268139-tgpsu4qz	197	6	were	be	VBD
cord-268139-tgpsu4qz	197	7	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	197	8	for	for	IN
cord-268139-tgpsu4qz	197	9	30	30	CD
cord-268139-tgpsu4qz	197	10	min	min	NN
cord-268139-tgpsu4qz	197	11	with	with	IN
cord-268139-tgpsu4qz	197	12	[	[	-LRB-
cord-268139-tgpsu4qz	197	13	35	35	CD
cord-268139-tgpsu4qz	197	14	S]Met	S]Met	NNP
cord-268139-tgpsu4qz	197	15	/	/	SYM
cord-268139-tgpsu4qz	197	16	Cys	Cys	NNP
cord-268139-tgpsu4qz	197	17	at	at	IN
cord-268139-tgpsu4qz	197	18	6	6	CD
cord-268139-tgpsu4qz	197	19	h	h	NN
cord-268139-tgpsu4qz	197	20	p.i	p.i	NNP
cord-268139-tgpsu4qz	197	21	.	.	NNP
cord-268139-tgpsu4qz	197	22	and	and	CC
cord-268139-tgpsu4qz	197	23	then	then	RB
cord-268139-tgpsu4qz	197	24	incubated	incubate	VBD
cord-268139-tgpsu4qz	197	25	in	in	IN
cord-268139-tgpsu4qz	197	26	medium	medium	JJ
cord-268139-tgpsu4qz	197	27	containing	contain	VBG
cord-268139-tgpsu4qz	197	28	cyclohexamide	cyclohexamide	NN
cord-268139-tgpsu4qz	197	29	for	for	IN
cord-268139-tgpsu4qz	197	30	15	15	CD
cord-268139-tgpsu4qz	197	31	to	to	TO
cord-268139-tgpsu4qz	197	32	360	360	CD
cord-268139-tgpsu4qz	197	33	min	min	NN
cord-268139-tgpsu4qz	197	34	as	as	IN
cord-268139-tgpsu4qz	197	35	described	describe	VBN
cord-268139-tgpsu4qz	197	36	in	in	IN
cord-268139-tgpsu4qz	197	37	Materials	material	NNS
cord-268139-tgpsu4qz	197	38	and	and	CC
cord-268139-tgpsu4qz	197	39	methods	method	NNS
cord-268139-tgpsu4qz	197	40	.	.	.
cord-268139-tgpsu4qz	198	1	Cells	cell	NNS
cord-268139-tgpsu4qz	198	2	were	be	VBD
cord-268139-tgpsu4qz	198	3	lysed	lyse	VBN
cord-268139-tgpsu4qz	198	4	at	at	IN
cord-268139-tgpsu4qz	198	5	the	the	DT
cord-268139-tgpsu4qz	198	6	indicated	indicate	VBN
cord-268139-tgpsu4qz	198	7	times	times	NNP
cord-268139-tgpsu4qz	198	8	(	(	-LRB-
cord-268139-tgpsu4qz	198	9	min	min	NNP
cord-268139-tgpsu4qz	198	10	)	)	-RRB-
cord-268139-tgpsu4qz	198	11	post	post	JJ
cord-268139-tgpsu4qz	198	12	-	-	JJ
cord-268139-tgpsu4qz	198	13	chase	chase	JJ
cord-268139-tgpsu4qz	198	14	(	(	-LRB-
cord-268139-tgpsu4qz	198	15	p.c	p.c	NNP
cord-268139-tgpsu4qz	198	16	.	.	NNP
cord-268139-tgpsu4qz	198	17	)	)	-RRB-
cord-268139-tgpsu4qz	198	18	,	,	,
cord-268139-tgpsu4qz	198	19	and	and	CC
cord-268139-tgpsu4qz	198	20	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	198	21	lysates	lysate	NNS
cord-268139-tgpsu4qz	198	22	were	be	VBD
cord-268139-tgpsu4qz	198	23	generated	generate	VBN
cord-268139-tgpsu4qz	198	24	for	for	IN
cord-268139-tgpsu4qz	198	25	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	198	26	studies	study	NNS
cord-268139-tgpsu4qz	198	27	using	use	VBG
cord-268139-tgpsu4qz	198	28	a	a	DT
cord-268139-tgpsu4qz	198	29	-	-	HYPH
cord-268139-tgpsu4qz	198	30	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	198	31	and	and	CC
cord-268139-tgpsu4qz	198	32	a	a	DT
cord-268139-tgpsu4qz	198	33	-	-	HYPH
cord-268139-tgpsu4qz	198	34	nsp2	nsp2	NN
cord-268139-tgpsu4qz	198	35	.	.	.
cord-268139-tgpsu4qz	199	1	Proteins	protein	NNS
cord-268139-tgpsu4qz	199	2	were	be	VBD
cord-268139-tgpsu4qz	199	3	analyzed	analyze	VBN
cord-268139-tgpsu4qz	199	4	as	as	IN
cord-268139-tgpsu4qz	199	5	in	in	IN
cord-268139-tgpsu4qz	199	6	Fig	Fig	NNP
cord-268139-tgpsu4qz	199	7	.	.	.
cord-268139-tgpsu4qz	200	1	3	3	LS
cord-268139-tgpsu4qz	200	2	.	.	.
cord-268139-tgpsu4qz	201	1	The	the	DT
cord-268139-tgpsu4qz	201	2	identities	identity	NNS
cord-268139-tgpsu4qz	201	3	of	of	IN
cord-268139-tgpsu4qz	201	4	proteins	protein	NNS
cord-268139-tgpsu4qz	201	5	are	be	VBP
cord-268139-tgpsu4qz	201	6	indicated	indicate	VBN
cord-268139-tgpsu4qz	201	7	to	to	IN
cord-268139-tgpsu4qz	201	8	the	the	DT
cord-268139-tgpsu4qz	201	9	right	right	NN
cord-268139-tgpsu4qz	201	10	of	of	IN
cord-268139-tgpsu4qz	201	11	the	the	DT
cord-268139-tgpsu4qz	201	12	fluorograms	fluorogram	NNS
cord-268139-tgpsu4qz	201	13	.	.	.
cord-268139-tgpsu4qz	202	1	The	the	DT
cord-268139-tgpsu4qz	202	2	number	number	NN
cord-268139-tgpsu4qz	202	3	of	of	IN
cord-268139-tgpsu4qz	202	4	days	day	NNS
cord-268139-tgpsu4qz	202	5	the	the	DT
cord-268139-tgpsu4qz	202	6	gels	gel	NNS
cord-268139-tgpsu4qz	202	7	were	be	VBD
cord-268139-tgpsu4qz	202	8	exposed	expose	VBN
cord-268139-tgpsu4qz	202	9	to	to	TO
cord-268139-tgpsu4qz	202	10	film	film	VB
cord-268139-tgpsu4qz	202	11	(	(	-LRB-
cord-268139-tgpsu4qz	202	12	d	d	FW
cord-268139-tgpsu4qz	202	13	exp	exp	NN
cord-268139-tgpsu4qz	202	14	)	)	-RRB-
cord-268139-tgpsu4qz	202	15	to	to	TO
cord-268139-tgpsu4qz	202	16	generate	generate	VB
cord-268139-tgpsu4qz	202	17	the	the	DT
cord-268139-tgpsu4qz	202	18	image	image	NN
cord-268139-tgpsu4qz	202	19	is	be	VBZ
cord-268139-tgpsu4qz	202	20	listed	list	VBN
cord-268139-tgpsu4qz	202	21	next	next	RB
cord-268139-tgpsu4qz	202	22	to	to	IN
cord-268139-tgpsu4qz	202	23	the	the	DT
cord-268139-tgpsu4qz	202	24	antisera	antisera	NN
cord-268139-tgpsu4qz	202	25	used	use	VBN
cord-268139-tgpsu4qz	202	26	for	for	IN
cord-268139-tgpsu4qz	202	27	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	202	28	.	.	.
cord-268139-tgpsu4qz	203	1	Pulse	pulse	NN
cord-268139-tgpsu4qz	203	2	-	-	HYPH
cord-268139-tgpsu4qz	203	3	chase	chase	NN
cord-268139-tgpsu4qz	203	4	translation	translation	NN
cord-268139-tgpsu4qz	203	5	in	in	IN
cord-268139-tgpsu4qz	203	6	cells	cell	NNS
cord-268139-tgpsu4qz	203	7	infected	infect	VBN
cord-268139-tgpsu4qz	203	8	with	with	IN
cord-268139-tgpsu4qz	203	9	(	(	-LRB-
cord-268139-tgpsu4qz	203	10	A	a	NN
cord-268139-tgpsu4qz	203	11	)	)	-RRB-
cord-268139-tgpsu4qz	203	12	icwt	icwt	NN
cord-268139-tgpsu4qz	203	13	or	or	CC
cord-268139-tgpsu4qz	203	14	(	(	-LRB-
cord-268139-tgpsu4qz	203	15	B	b	NN
cord-268139-tgpsu4qz	203	16	)	)	-RRB-
cord-268139-tgpsu4qz	203	17	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	203	18	virus	virus	NN
cord-268139-tgpsu4qz	203	19	.	.	.
cord-268139-tgpsu4qz	203	20	.	.	.
cord-268139-tgpsu4qz	204	1	Intracellular	Intracellular	NNP
cord-268139-tgpsu4qz	204	2	localization	localization	NN
cord-268139-tgpsu4qz	204	3	of	of	IN
cord-268139-tgpsu4qz	204	4	mutant	mutant	JJ
cord-268139-tgpsu4qz	204	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	204	6	proteins	protein	NNS
cord-268139-tgpsu4qz	204	7	.	.	.
cord-268139-tgpsu4qz	205	1	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	205	2	cells	cell	NNS
cord-268139-tgpsu4qz	205	3	grown	grow	VBN
cord-268139-tgpsu4qz	205	4	on	on	IN
cord-268139-tgpsu4qz	205	5	glass	glass	NN
cord-268139-tgpsu4qz	205	6	coverslips	coverslip	NNS
cord-268139-tgpsu4qz	205	7	were	be	VBD
cord-268139-tgpsu4qz	205	8	infected	infect	VBN
cord-268139-tgpsu4qz	205	9	with	with	IN
cord-268139-tgpsu4qz	205	10	icwt	icwt	NNP
cord-268139-tgpsu4qz	205	11	or	or	CC
cord-268139-tgpsu4qz	205	12	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	205	13	mutant	mutant	JJ
cord-268139-tgpsu4qz	205	14	viruses	virus	NNS
cord-268139-tgpsu4qz	205	15	for	for	IN
cord-268139-tgpsu4qz	205	16	7	7	CD
cord-268139-tgpsu4qz	205	17	h	h	NN
cord-268139-tgpsu4qz	205	18	,	,	,
cord-268139-tgpsu4qz	205	19	fixed	fix	VBN
cord-268139-tgpsu4qz	205	20	and	and	CC
cord-268139-tgpsu4qz	205	21	permeablized	permeablize	VBN
cord-268139-tgpsu4qz	205	22	with	with	IN
cord-268139-tgpsu4qz	205	23	100	100	CD
cord-268139-tgpsu4qz	205	24	%	%	NN
cord-268139-tgpsu4qz	205	25	methanol	methanol	NN
cord-268139-tgpsu4qz	205	26	,	,	,
cord-268139-tgpsu4qz	205	27	and	and	CC
cord-268139-tgpsu4qz	205	28	incubated	incubate	VBN
cord-268139-tgpsu4qz	205	29	with	with	IN
cord-268139-tgpsu4qz	205	30	antibodies	antibody	NNS
cord-268139-tgpsu4qz	205	31	against	against	IN
cord-268139-tgpsu4qz	205	32	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	205	33	(	(	-LRB-
cord-268139-tgpsu4qz	205	34	red	red	NNP
cord-268139-tgpsu4qz	205	35	)	)	-RRB-
cord-268139-tgpsu4qz	205	36	,	,	,
cord-268139-tgpsu4qz	205	37	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	205	38	(	(	-LRB-
cord-268139-tgpsu4qz	205	39	green	green	NNP
cord-268139-tgpsu4qz	205	40	)	)	-RRB-
cord-268139-tgpsu4qz	205	41	,	,	,
cord-268139-tgpsu4qz	205	42	and	and	CC
cord-268139-tgpsu4qz	205	43	N	N	NNP
cord-268139-tgpsu4qz	205	44	(	(	-LRB-
cord-268139-tgpsu4qz	205	45	purple	purple	NN
cord-268139-tgpsu4qz	205	46	)	)	-RRB-
cord-268139-tgpsu4qz	205	47	.	.	.
cord-268139-tgpsu4qz	206	1	Cells	cell	NNS
cord-268139-tgpsu4qz	206	2	were	be	VBD
cord-268139-tgpsu4qz	206	3	imaged	image	VBN
cord-268139-tgpsu4qz	206	4	using	use	VBG
cord-268139-tgpsu4qz	206	5	a	a	DT
cord-268139-tgpsu4qz	206	6	Zeiss	Zeiss	NNP
cord-268139-tgpsu4qz	206	7	LSM	LSM	NNP
cord-268139-tgpsu4qz	206	8	510	510	CD
cord-268139-tgpsu4qz	206	9	confocal	confocal	JJ
cord-268139-tgpsu4qz	206	10	microscope	microscope	NN
cord-268139-tgpsu4qz	206	11	at	at	IN
cord-268139-tgpsu4qz	206	12	546	546	CD
cord-268139-tgpsu4qz	206	13	nm	nm	NN
cord-268139-tgpsu4qz	206	14	(	(	-LRB-
cord-268139-tgpsu4qz	206	15	red	red	NNP
cord-268139-tgpsu4qz	206	16	)	)	-RRB-
cord-268139-tgpsu4qz	206	17	,	,	,
cord-268139-tgpsu4qz	206	18	488	488	CD
cord-268139-tgpsu4qz	206	19	nm	nm	NN
cord-268139-tgpsu4qz	206	20	(	(	-LRB-
cord-268139-tgpsu4qz	206	21	green	green	NNP
cord-268139-tgpsu4qz	206	22	)	)	-RRB-
cord-268139-tgpsu4qz	206	23	,	,	,
cord-268139-tgpsu4qz	206	24	and	and	CC
cord-268139-tgpsu4qz	206	25	633	633	CD
cord-268139-tgpsu4qz	206	26	nm	nm	NN
cord-268139-tgpsu4qz	206	27	(	(	-LRB-
cord-268139-tgpsu4qz	206	28	purple	purple	NN
cord-268139-tgpsu4qz	206	29	)	)	-RRB-
cord-268139-tgpsu4qz	206	30	.	.	.
cord-268139-tgpsu4qz	207	1	Images	image	NNS
cord-268139-tgpsu4qz	207	2	are	be	VBP
cord-268139-tgpsu4qz	207	3	single	single	JJ
cord-268139-tgpsu4qz	207	4	confocal	confocal	JJ
cord-268139-tgpsu4qz	207	5	slices	slice	NNS
cord-268139-tgpsu4qz	207	6	obtained	obtain	VBN
cord-268139-tgpsu4qz	207	7	using	use	VBG
cord-268139-tgpsu4qz	207	8	a	a	DT
cord-268139-tgpsu4qz	207	9	40Â	40â	CD
cord-268139-tgpsu4qz	207	10	objective	objective	NN
cord-268139-tgpsu4qz	207	11	.	.	.
cord-268139-tgpsu4qz	208	1	Co	co	NN
cord-268139-tgpsu4qz	208	2	-	-	NN
cord-268139-tgpsu4qz	208	3	localization	localization	NN
cord-268139-tgpsu4qz	208	4	of	of	IN
cord-268139-tgpsu4qz	208	5	green	green	JJ
cord-268139-tgpsu4qz	208	6	and	and	CC
cord-268139-tgpsu4qz	208	7	purple	purple	NN
cord-268139-tgpsu4qz	208	8	for	for	IN
cord-268139-tgpsu4qz	208	9	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	208	10	is	be	VBZ
cord-268139-tgpsu4qz	208	11	shown	show	VBN
cord-268139-tgpsu4qz	208	12	in	in	IN
cord-268139-tgpsu4qz	208	13	the	the	DT
cord-268139-tgpsu4qz	208	14	merged	merged	JJ
cord-268139-tgpsu4qz	208	15	image	image	NN
cord-268139-tgpsu4qz	208	16	as	as	IN
cord-268139-tgpsu4qz	208	17	light	light	JJ
cord-268139-tgpsu4qz	208	18	green	green	JJ
cord-268139-tgpsu4qz	208	19	pixels	pixel	NNS
cord-268139-tgpsu4qz	208	20	.	.	.
cord-268139-tgpsu4qz	209	1	Co	co	NN
cord-268139-tgpsu4qz	209	2	-	-	NN
cord-268139-tgpsu4qz	209	3	localization	localization	NN
cord-268139-tgpsu4qz	209	4	of	of	IN
cord-268139-tgpsu4qz	209	5	all	all	DT
cord-268139-tgpsu4qz	209	6	three	three	CD
cord-268139-tgpsu4qz	209	7	colors	color	NNS
cord-268139-tgpsu4qz	209	8	is	be	VBZ
cord-268139-tgpsu4qz	209	9	shown	show	VBN
cord-268139-tgpsu4qz	209	10	in	in	IN
cord-268139-tgpsu4qz	209	11	the	the	DT
cord-268139-tgpsu4qz	209	12	merged	merge	VBN
cord-268139-tgpsu4qz	209	13	images	image	NNS
cord-268139-tgpsu4qz	209	14	as	as	IN
cord-268139-tgpsu4qz	209	15	white	white	JJ
cord-268139-tgpsu4qz	209	16	pixels	pixel	NNS
cord-268139-tgpsu4qz	209	17	.	.	.
cord-268139-tgpsu4qz	210	1	Multi	multi	JJ
cord-268139-tgpsu4qz	210	2	-	-	JJ
cord-268139-tgpsu4qz	210	3	nucleated	nucleated	JJ
cord-268139-tgpsu4qz	210	4	cells	cell	NNS
cord-268139-tgpsu4qz	210	5	are	be	VBP
cord-268139-tgpsu4qz	210	6	a	a	DT
cord-268139-tgpsu4qz	210	7	cytopathic	cytopathic	JJ
cord-268139-tgpsu4qz	210	8	effect	effect	NN
cord-268139-tgpsu4qz	210	9	of	of	IN
cord-268139-tgpsu4qz	210	10	MHV	MHV	NNP
cord-268139-tgpsu4qz	210	11	replication	replication	NN
cord-268139-tgpsu4qz	210	12	.	.	.
cord-268139-tgpsu4qz	211	1	the	the	DT
cord-268139-tgpsu4qz	211	2	30	30	CD
cord-268139-tgpsu4qz	211	3	min	min	NN
cord-268139-tgpsu4qz	211	4	period	period	NN
cord-268139-tgpsu4qz	211	5	of	of	IN
cord-268139-tgpsu4qz	211	6	radiolabel	radiolabel	NNP
cord-268139-tgpsu4qz	211	7	.	.	.
cord-268139-tgpsu4qz	212	1	However	however	RB
cord-268139-tgpsu4qz	212	2	,	,	,
cord-268139-tgpsu4qz	212	3	in	in	IN
cord-268139-tgpsu4qz	212	4	contrast	contrast	NN
cord-268139-tgpsu4qz	212	5	to	to	IN
cord-268139-tgpsu4qz	212	6	icwt	icwt	NNP
cord-268139-tgpsu4qz	212	7	nsp1	nsp1	NNP
cord-268139-tgpsu4qz	212	8	,	,	,
cord-268139-tgpsu4qz	212	9	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	212	10	was	be	VBD
cord-268139-tgpsu4qz	212	11	not	not	RB
cord-268139-tgpsu4qz	212	12	detectable	detectable	JJ
cord-268139-tgpsu4qz	212	13	after	after	IN
cord-268139-tgpsu4qz	212	14	120	120	CD
cord-268139-tgpsu4qz	212	15	min	min	NN
cord-268139-tgpsu4qz	212	16	p.c	p.c	NNP
cord-268139-tgpsu4qz	212	17	.	.	NNP
cord-268139-tgpsu4qz	212	18	,	,	,
cord-268139-tgpsu4qz	212	19	suggesting	suggest	VBG
cord-268139-tgpsu4qz	212	20	that	that	IN
cord-268139-tgpsu4qz	212	21	once	once	RB
cord-268139-tgpsu4qz	212	22	processed	process	VBD
cord-268139-tgpsu4qz	212	23	the	the	DT
cord-268139-tgpsu4qz	212	24	protein	protein	NN
cord-268139-tgpsu4qz	212	25	may	may	MD
cord-268139-tgpsu4qz	212	26	be	be	VB
cord-268139-tgpsu4qz	212	27	subject	subject	JJ
cord-268139-tgpsu4qz	212	28	to	to	IN
cord-268139-tgpsu4qz	212	29	degradation	degradation	NN
cord-268139-tgpsu4qz	212	30	.	.	.
cord-268139-tgpsu4qz	213	1	The	the	DT
cord-268139-tgpsu4qz	213	2	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	213	3	of	of	IN
cord-268139-tgpsu4qz	213	4	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	213	5	expression	expression	NN
cord-268139-tgpsu4qz	213	6	in	in	IN
cord-268139-tgpsu4qz	213	7	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	213	8	virus	virus	NN
cord-268139-tgpsu4qz	213	9	-	-	HYPH
cord-268139-tgpsu4qz	213	10	infected	infect	VBN
cord-268139-tgpsu4qz	213	11	cells	cell	NNS
cord-268139-tgpsu4qz	213	12	were	be	VBD
cord-268139-tgpsu4qz	213	13	similar	similar	JJ
cord-268139-tgpsu4qz	213	14	to	to	IN
cord-268139-tgpsu4qz	213	15	those	those	DT
cord-268139-tgpsu4qz	213	16	of	of	IN
cord-268139-tgpsu4qz	213	17	nsp2	nsp2	NN
cord-268139-tgpsu4qz	213	18	from	from	IN
cord-268139-tgpsu4qz	213	19	icwt	icwt	NNP
cord-268139-tgpsu4qz	213	20	(	(	-LRB-
cord-268139-tgpsu4qz	213	21	Fig	Fig	NNP
cord-268139-tgpsu4qz	213	22	.	.	.
cord-268139-tgpsu4qz	213	23	4B	4b	LS
cord-268139-tgpsu4qz	213	24	)	)	-RRB-
cord-268139-tgpsu4qz	213	25	;	;	:
cord-268139-tgpsu4qz	213	26	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	213	27	(	(	-LRB-
cord-268139-tgpsu4qz	213	28	65	65	CD
cord-268139-tgpsu4qz	213	29	kDa	kDa	NNP
cord-268139-tgpsu4qz	213	30	)	)	-RRB-
cord-268139-tgpsu4qz	213	31	was	be	VBD
cord-268139-tgpsu4qz	213	32	first	first	RB
cord-268139-tgpsu4qz	213	33	detected	detect	VBN
cord-268139-tgpsu4qz	213	34	at	at	IN
cord-268139-tgpsu4qz	213	35	0	0	CD
cord-268139-tgpsu4qz	213	36	-	-	SYM
cord-268139-tgpsu4qz	213	37	15	15	CD
cord-268139-tgpsu4qz	213	38	min	min	NNP
cord-268139-tgpsu4qz	213	39	p.c	p.c	NNP
cord-268139-tgpsu4qz	213	40	.	.	NNP
cord-268139-tgpsu4qz	213	41	,	,	,
cord-268139-tgpsu4qz	213	42	but	but	CC
cord-268139-tgpsu4qz	213	43	detection	detection	NN
cord-268139-tgpsu4qz	213	44	was	be	VBD
cord-268139-tgpsu4qz	213	45	increased	increase	VBN
cord-268139-tgpsu4qz	213	46	at	at	IN
cord-268139-tgpsu4qz	213	47	60	60	CD
cord-268139-tgpsu4qz	213	48	-360	-360	NN
cord-268139-tgpsu4qz	213	49	min	min	NNP
cord-268139-tgpsu4qz	213	50	p.c	p.c	NNP
cord-268139-tgpsu4qz	213	51	.	.	.
cord-268139-tgpsu4qz	214	1	Using	use	VBG
cord-268139-tgpsu4qz	214	2	either	either	CC
cord-268139-tgpsu4qz	214	3	a	a	DT
cord-268139-tgpsu4qz	214	4	-	-	HYPH
cord-268139-tgpsu4qz	214	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	214	6	or	or	CC
cord-268139-tgpsu4qz	214	7	a	a	DT
cord-268139-tgpsu4qz	214	8	-	-	HYPH
cord-268139-tgpsu4qz	214	9	nsp2	nsp2	NN
cord-268139-tgpsu4qz	214	10	,	,	,
cord-268139-tgpsu4qz	214	11	the	the	DT
cord-268139-tgpsu4qz	214	12	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	214	13	protein	protein	NN
cord-268139-tgpsu4qz	214	14	was	be	VBD
cord-268139-tgpsu4qz	214	15	detectable	detectable	JJ
cord-268139-tgpsu4qz	214	16	by	by	IN
cord-268139-tgpsu4qz	214	17	0	0	CD
cord-268139-tgpsu4qz	214	18	min	min	NNP
cord-268139-tgpsu4qz	214	19	p.c	p.c	NNP
cord-268139-tgpsu4qz	214	20	.	.	NNP
cord-268139-tgpsu4qz	214	21	and	and	CC
cord-268139-tgpsu4qz	214	22	remained	remain	VBD
cord-268139-tgpsu4qz	214	23	stable	stable	JJ
cord-268139-tgpsu4qz	214	24	or	or	CC
cord-268139-tgpsu4qz	214	25	increasing	increase	VBG
cord-268139-tgpsu4qz	214	26	until	until	IN
cord-268139-tgpsu4qz	214	27	240	240	CD
cord-268139-tgpsu4qz	214	28	min	min	NN
cord-268139-tgpsu4qz	215	1	p.c	p.c	NNP
cord-268139-tgpsu4qz	215	2	.	.	.
cord-268139-tgpsu4qz	216	1	At	at	IN
cord-268139-tgpsu4qz	216	2	360	360	CD
cord-268139-tgpsu4qz	216	3	min	min	NN
cord-268139-tgpsu4qz	216	4	p.c	p.c	NNP
cord-268139-tgpsu4qz	216	5	.	.	NNP
cord-268139-tgpsu4qz	216	6	,	,	,
cord-268139-tgpsu4qz	216	7	the	the	DT
cord-268139-tgpsu4qz	216	8	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	216	9	band	band	NN
cord-268139-tgpsu4qz	216	10	was	be	VBD
cord-268139-tgpsu4qz	216	11	slightly	slightly	RB
cord-268139-tgpsu4qz	216	12	less	less	RBR
cord-268139-tgpsu4qz	216	13	intense	intense	JJ
cord-268139-tgpsu4qz	216	14	;	;	:
cord-268139-tgpsu4qz	216	15	however	however	RB
cord-268139-tgpsu4qz	216	16	,	,	,
cord-268139-tgpsu4qz	216	17	at	at	IN
cord-268139-tgpsu4qz	216	18	this	this	DT
cord-268139-tgpsu4qz	216	19	time	time	NN
cord-268139-tgpsu4qz	216	20	point	point	NN
cord-268139-tgpsu4qz	216	21	,	,	,
cord-268139-tgpsu4qz	216	22	there	there	EX
cord-268139-tgpsu4qz	216	23	was	be	VBD
cord-268139-tgpsu4qz	216	24	no	no	DT
cord-268139-tgpsu4qz	216	25	concurrent	concurrent	JJ
cord-268139-tgpsu4qz	216	26	increase	increase	NN
cord-268139-tgpsu4qz	216	27	in	in	IN
cord-268139-tgpsu4qz	216	28	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	216	29	or	or	CC
cord-268139-tgpsu4qz	216	30	nsp2	nsp2	NN
cord-268139-tgpsu4qz	216	31	detection	detection	NN
cord-268139-tgpsu4qz	216	32	.	.	.
cord-268139-tgpsu4qz	217	1	This	this	DT
cord-268139-tgpsu4qz	217	2	result	result	NN
cord-268139-tgpsu4qz	217	3	suggests	suggest	VBZ
cord-268139-tgpsu4qz	217	4	that	that	IN
cord-268139-tgpsu4qz	217	5	the	the	DT
cord-268139-tgpsu4qz	217	6	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	217	7	protein	protein	NN
cord-268139-tgpsu4qz	217	8	may	may	MD
cord-268139-tgpsu4qz	217	9	be	be	VB
cord-268139-tgpsu4qz	217	10	degraded	degrade	VBN
cord-268139-tgpsu4qz	217	11	at	at	IN
cord-268139-tgpsu4qz	217	12	late	late	JJ
cord-268139-tgpsu4qz	217	13	times	time	NNS
cord-268139-tgpsu4qz	217	14	of	of	IN
cord-268139-tgpsu4qz	217	15	chase	chase	NNP
cord-268139-tgpsu4qz	217	16	rather	rather	RB
cord-268139-tgpsu4qz	217	17	than	than	IN
cord-268139-tgpsu4qz	217	18	processed	process	VBN
cord-268139-tgpsu4qz	217	19	.	.	.
cord-268139-tgpsu4qz	218	1	As	as	IN
cord-268139-tgpsu4qz	218	2	with	with	IN
cord-268139-tgpsu4qz	218	3	icwt	icwt	NNP
cord-268139-tgpsu4qz	218	4	,	,	,
cord-268139-tgpsu4qz	218	5	the	the	DT
cord-268139-tgpsu4qz	218	6	high	high	JJ
cord-268139-tgpsu4qz	218	7	molecular	molecular	JJ
cord-268139-tgpsu4qz	218	8	weight	weight	NN
cord-268139-tgpsu4qz	218	9	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	218	10	viral	viral	JJ
cord-268139-tgpsu4qz	218	11	precursor	precursor	NN
cord-268139-tgpsu4qz	218	12	proteins	protein	NNS
cord-268139-tgpsu4qz	218	13	were	be	VBD
cord-268139-tgpsu4qz	218	14	not	not	RB
cord-268139-tgpsu4qz	218	15	detected	detect	VBN
cord-268139-tgpsu4qz	218	16	by	by	IN
cord-268139-tgpsu4qz	218	17	the	the	DT
cord-268139-tgpsu4qz	218	18	antisera	antisera	NN
cord-268139-tgpsu4qz	218	19	.	.	.
cord-268139-tgpsu4qz	219	1	During	during	IN
cord-268139-tgpsu4qz	219	2	early	early	JJ
cord-268139-tgpsu4qz	219	3	times	time	NNS
cord-268139-tgpsu4qz	219	4	of	of	IN
cord-268139-tgpsu4qz	219	5	infection	infection	NN
cord-268139-tgpsu4qz	219	6	(	(	-LRB-
cord-268139-tgpsu4qz	219	7	4	4	CD
cord-268139-tgpsu4qz	219	8	-7	-7	NNP
cord-268139-tgpsu4qz	219	9	h	h	NNP
cord-268139-tgpsu4qz	219	10	p.i	p.i	NNP
cord-268139-tgpsu4qz	219	11	.	.	NNP
cord-268139-tgpsu4qz	219	12	)	)	-RRB-
cord-268139-tgpsu4qz	219	13	,	,	,
cord-268139-tgpsu4qz	219	14	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	219	15	colocalizes	colocalizes	NNP
cord-268139-tgpsu4qz	219	16	with	with	IN
cord-268139-tgpsu4qz	219	17	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	219	18	and	and	CC
cord-268139-tgpsu4qz	219	19	the	the	DT
cord-268139-tgpsu4qz	219	20	viral	viral	JJ
cord-268139-tgpsu4qz	219	21	nucleocapsid	nucleocapsid	NNP
cord-268139-tgpsu4qz	219	22	protein	protein	NNP
cord-268139-tgpsu4qz	219	23	(	(	-LRB-
cord-268139-tgpsu4qz	219	24	N	N	NNP
cord-268139-tgpsu4qz	219	25	)	)	-RRB-
cord-268139-tgpsu4qz	219	26	at	at	IN
cord-268139-tgpsu4qz	219	27	viral	viral	JJ
cord-268139-tgpsu4qz	219	28	replication	replication	NN
cord-268139-tgpsu4qz	219	29	complexes	complex	NNS
cord-268139-tgpsu4qz	219	30	in	in	IN
cord-268139-tgpsu4qz	219	31	the	the	DT
cord-268139-tgpsu4qz	219	32	cytoplasm	cytoplasm	NN
cord-268139-tgpsu4qz	219	33	.	.	.
cord-268139-tgpsu4qz	220	1	At	at	IN
cord-268139-tgpsu4qz	220	2	these	these	DT
cord-268139-tgpsu4qz	220	3	times	time	NNS
cord-268139-tgpsu4qz	220	4	,	,	,
cord-268139-tgpsu4qz	220	5	nsp1	nsp1	CD
cord-268139-tgpsu4qz	220	6	is	be	VBZ
cord-268139-tgpsu4qz	220	7	mostly	mostly	RB
cord-268139-tgpsu4qz	220	8	distinct	distinct	JJ
cord-268139-tgpsu4qz	220	9	from	from	IN
cord-268139-tgpsu4qz	220	10	the	the	DT
cord-268139-tgpsu4qz	220	11	virion	virion	NN
cord-268139-tgpsu4qz	220	12	membrane	membrane	NN
cord-268139-tgpsu4qz	220	13	protein	protein	NN
cord-268139-tgpsu4qz	220	14	(	(	-LRB-
cord-268139-tgpsu4qz	220	15	M	M	NNP
cord-268139-tgpsu4qz	220	16	)	)	-RRB-
cord-268139-tgpsu4qz	220	17	,	,	,
cord-268139-tgpsu4qz	220	18	which	which	WDT
cord-268139-tgpsu4qz	220	19	localizes	localize	VBZ
cord-268139-tgpsu4qz	220	20	to	to	IN
cord-268139-tgpsu4qz	220	21	sites	site	NNS
cord-268139-tgpsu4qz	220	22	of	of	IN
cord-268139-tgpsu4qz	220	23	particle	particle	NN
cord-268139-tgpsu4qz	220	24	assembly	assembly	NN
cord-268139-tgpsu4qz	220	25	.	.	.
cord-268139-tgpsu4qz	221	1	To	to	TO
cord-268139-tgpsu4qz	221	2	determine	determine	VB
cord-268139-tgpsu4qz	221	3	the	the	DT
cord-268139-tgpsu4qz	221	4	intracellular	intracellular	JJ
cord-268139-tgpsu4qz	221	5	localization	localization	NN
cord-268139-tgpsu4qz	221	6	of	of	IN
cord-268139-tgpsu4qz	221	7	viral	viral	JJ
cord-268139-tgpsu4qz	221	8	proteins	protein	NNS
cord-268139-tgpsu4qz	221	9	from	from	IN
cord-268139-tgpsu4qz	221	10	the	the	DT
cord-268139-tgpsu4qz	221	11	nsp1	nsp1	CD
cord-268139-tgpsu4qz	221	12	mutants	mutant	NNS
cord-268139-tgpsu4qz	221	13	,	,	,
cord-268139-tgpsu4qz	221	14	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	221	15	confocal	confocal	JJ
cord-268139-tgpsu4qz	221	16	microscopy	microscopy	NNS
cord-268139-tgpsu4qz	221	17	was	be	VBD
cord-268139-tgpsu4qz	221	18	performed	perform	VBN
cord-268139-tgpsu4qz	221	19	.	.	.
cord-268139-tgpsu4qz	222	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	222	2	cells	cell	NNS
cord-268139-tgpsu4qz	222	3	on	on	IN
cord-268139-tgpsu4qz	222	4	glass	glass	NN
cord-268139-tgpsu4qz	222	5	coverslips	coverslip	NNS
cord-268139-tgpsu4qz	222	6	were	be	VBD
cord-268139-tgpsu4qz	222	7	infected	infect	VBN
cord-268139-tgpsu4qz	222	8	for	for	IN
cord-268139-tgpsu4qz	222	9	7	7	CD
cord-268139-tgpsu4qz	222	10	h	h	NN
cord-268139-tgpsu4qz	222	11	,	,	,
cord-268139-tgpsu4qz	222	12	fixed	fix	VBN
cord-268139-tgpsu4qz	222	13	and	and	CC
cord-268139-tgpsu4qz	222	14	permeabilized	permeabilize	VBN
cord-268139-tgpsu4qz	222	15	with	with	IN
cord-268139-tgpsu4qz	222	16	methanol	methanol	NN
cord-268139-tgpsu4qz	222	17	,	,	,
cord-268139-tgpsu4qz	222	18	and	and	CC
cord-268139-tgpsu4qz	222	19	incubated	incubate	VBN
cord-268139-tgpsu4qz	222	20	with	with	IN
cord-268139-tgpsu4qz	222	21	antisera	antisera	NN
cord-268139-tgpsu4qz	222	22	against	against	IN
cord-268139-tgpsu4qz	222	23	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	222	24	,	,	,
cord-268139-tgpsu4qz	222	25	nsp2	nsp2	CC
cord-268139-tgpsu4qz	222	26	,	,	,
cord-268139-tgpsu4qz	222	27	and	and	CC
cord-268139-tgpsu4qz	222	28	either	either	CC
cord-268139-tgpsu4qz	222	29	N	N	NNP
cord-268139-tgpsu4qz	222	30	or	or	CC
cord-268139-tgpsu4qz	222	31	M	M	NNP
cord-268139-tgpsu4qz	222	32	(	(	-LRB-
cord-268139-tgpsu4qz	222	33	Fig	Fig	NNP
cord-268139-tgpsu4qz	222	34	.	.	.
cord-268139-tgpsu4qz	222	35	5	5	CD
cord-268139-tgpsu4qz	222	36	)	)	-RRB-
cord-268139-tgpsu4qz	222	37	.	.	.
cord-268139-tgpsu4qz	223	1	The	the	DT
cord-268139-tgpsu4qz	223	2	a	a	DT
cord-268139-tgpsu4qz	223	3	-	-	HYPH
cord-268139-tgpsu4qz	223	4	nsp1	nsp1	NN
cord-268139-tgpsu4qz	223	5	staining	staining	NN
cord-268139-tgpsu4qz	223	6	pattern	pattern	NN
cord-268139-tgpsu4qz	223	7	in	in	IN
cord-268139-tgpsu4qz	223	8	cells	cell	NNS
cord-268139-tgpsu4qz	223	9	infected	infect	VBN
cord-268139-tgpsu4qz	223	10	with	with	IN
cord-268139-tgpsu4qz	223	11	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	223	12	,	,	,
cord-268139-tgpsu4qz	223	13	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	223	14	,	,	,
cord-268139-tgpsu4qz	223	15	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	223	16	,	,	,
cord-268139-tgpsu4qz	223	17	VUSB7	VUSB7	NNP
cord-268139-tgpsu4qz	223	18	,	,	,
cord-268139-tgpsu4qz	223	19	or	or	CC
cord-268139-tgpsu4qz	223	20	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	223	21	was	be	VBD
cord-268139-tgpsu4qz	223	22	similar	similar	JJ
cord-268139-tgpsu4qz	223	23	to	to	IN
cord-268139-tgpsu4qz	223	24	icwt	icwt	NNP
cord-268139-tgpsu4qz	223	25	.	.	.
cord-268139-tgpsu4qz	224	1	Nsp1	Nsp1	NNS
cord-268139-tgpsu4qz	224	2	from	from	IN
cord-268139-tgpsu4qz	224	3	these	these	DT
cord-268139-tgpsu4qz	224	4	mutant	mutant	JJ
cord-268139-tgpsu4qz	224	5	viruses	virus	NNS
cord-268139-tgpsu4qz	224	6	co	co	VBD
cord-268139-tgpsu4qz	224	7	-	-	VBN
cord-268139-tgpsu4qz	224	8	localized	localize	VBN
cord-268139-tgpsu4qz	224	9	with	with	IN
cord-268139-tgpsu4qz	224	10	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	224	11	and	and	CC
cord-268139-tgpsu4qz	224	12	N	N	NNP
cord-268139-tgpsu4qz	224	13	in	in	IN
cord-268139-tgpsu4qz	224	14	punctate	punctate	JJ
cord-268139-tgpsu4qz	224	15	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	224	16	replication	replication	NN
cord-268139-tgpsu4qz	224	17	complexes	complex	NNS
cord-268139-tgpsu4qz	224	18	(	(	-LRB-
cord-268139-tgpsu4qz	224	19	Fig	Fig	NNP
cord-268139-tgpsu4qz	224	20	.	.	.
cord-268139-tgpsu4qz	224	21	5	5	CD
cord-268139-tgpsu4qz	224	22	)	)	-RRB-
cord-268139-tgpsu4qz	224	23	.	.	.
cord-268139-tgpsu4qz	225	1	At	at	IN
cord-268139-tgpsu4qz	225	2	this	this	DT
cord-268139-tgpsu4qz	225	3	time	time	NN
cord-268139-tgpsu4qz	225	4	point	point	NN
cord-268139-tgpsu4qz	225	5	,	,	,
cord-268139-tgpsu4qz	225	6	the	the	DT
cord-268139-tgpsu4qz	225	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	225	8	proteins	protein	NNS
cord-268139-tgpsu4qz	225	9	from	from	IN
cord-268139-tgpsu4qz	225	10	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	225	11	,	,	,
cord-268139-tgpsu4qz	225	12	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	225	13	,	,	,
cord-268139-tgpsu4qz	225	14	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	225	15	,	,	,
cord-268139-tgpsu4qz	225	16	VUSB7	VUSB7	NNP
cord-268139-tgpsu4qz	225	17	,	,	,
cord-268139-tgpsu4qz	225	18	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	225	19	,	,	,
cord-268139-tgpsu4qz	225	20	or	or	CC
cord-268139-tgpsu4qz	225	21	icwt	icwt	JJ
cord-268139-tgpsu4qz	225	22	viruses	virus	NNS
cord-268139-tgpsu4qz	225	23	were	be	VBD
cord-268139-tgpsu4qz	225	24	also	also	RB
cord-268139-tgpsu4qz	225	25	distinct	distinct	JJ
cord-268139-tgpsu4qz	225	26	from	from	IN
cord-268139-tgpsu4qz	225	27	sites	site	NNS
cord-268139-tgpsu4qz	225	28	of	of	IN
cord-268139-tgpsu4qz	225	29	virion	virion	NN
cord-268139-tgpsu4qz	225	30	assembly	assembly	NN
cord-268139-tgpsu4qz	225	31	as	as	IN
cord-268139-tgpsu4qz	225	32	determined	determine	VBN
cord-268139-tgpsu4qz	225	33	by	by	IN
cord-268139-tgpsu4qz	225	34	lack	lack	NN
cord-268139-tgpsu4qz	225	35	of	of	IN
cord-268139-tgpsu4qz	225	36	predominant	predominant	JJ
cord-268139-tgpsu4qz	225	37	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	225	38	co	co	NN
cord-268139-tgpsu4qz	225	39	-	-	NN
cord-268139-tgpsu4qz	225	40	localization	localization	NN
cord-268139-tgpsu4qz	225	41	with	with	IN
cord-268139-tgpsu4qz	225	42	M	M	NNP
cord-268139-tgpsu4qz	225	43	(	(	-LRB-
cord-268139-tgpsu4qz	225	44	data	datum	NNS
cord-268139-tgpsu4qz	225	45	not	not	RB
cord-268139-tgpsu4qz	225	46	shown	show	VBN
cord-268139-tgpsu4qz	225	47	)	)	-RRB-
cord-268139-tgpsu4qz	225	48	.	.	.
cord-268139-tgpsu4qz	226	1	Using	use	VBG
cord-268139-tgpsu4qz	226	2	the	the	DT
cord-268139-tgpsu4qz	226	3	a	a	DT
cord-268139-tgpsu4qz	226	4	-	-	HYPH
cord-268139-tgpsu4qz	226	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	226	6	antisera	antisera	NN
cord-268139-tgpsu4qz	226	7	,	,	,
cord-268139-tgpsu4qz	226	8	no	no	DT
cord-268139-tgpsu4qz	226	9	specific	specific	JJ
cord-268139-tgpsu4qz	226	10	staining	staining	NN
cord-268139-tgpsu4qz	226	11	above	above	IN
cord-268139-tgpsu4qz	226	12	background	background	NN
cord-268139-tgpsu4qz	226	13	levels	level	NNS
cord-268139-tgpsu4qz	226	14	was	be	VBD
cord-268139-tgpsu4qz	226	15	detected	detect	VBN
cord-268139-tgpsu4qz	226	16	in	in	IN
cord-268139-tgpsu4qz	226	17	VUSB1-infected	vusb1-infected	JJ
cord-268139-tgpsu4qz	226	18	cells	cell	NNS
cord-268139-tgpsu4qz	226	19	,	,	,
cord-268139-tgpsu4qz	226	20	whereas	whereas	IN
cord-268139-tgpsu4qz	226	21	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	226	22	,	,	,
cord-268139-tgpsu4qz	226	23	N	N	NNP
cord-268139-tgpsu4qz	226	24	,	,	,
cord-268139-tgpsu4qz	226	25	and	and	CC
cord-268139-tgpsu4qz	226	26	M	M	NNP
cord-268139-tgpsu4qz	226	27	staining	staining	NN
cord-268139-tgpsu4qz	226	28	was	be	VBD
cord-268139-tgpsu4qz	226	29	indistinguishable	indistinguishable	JJ
cord-268139-tgpsu4qz	226	30	from	from	IN
cord-268139-tgpsu4qz	226	31	icwt	icwt	NNP
cord-268139-tgpsu4qz	226	32	(	(	-LRB-
cord-268139-tgpsu4qz	226	33	Fig	Fig	NNP
cord-268139-tgpsu4qz	226	34	.	.	.
cord-268139-tgpsu4qz	227	1	5	5	CD
cord-268139-tgpsu4qz	227	2	and	and	CC
cord-268139-tgpsu4qz	227	3	data	datum	NNS
cord-268139-tgpsu4qz	227	4	not	not	RB
cord-268139-tgpsu4qz	227	5	shown	show	VBN
cord-268139-tgpsu4qz	227	6	)	)	-RRB-
cord-268139-tgpsu4qz	227	7	.	.	.
cord-268139-tgpsu4qz	228	1	The	the	DT
cord-268139-tgpsu4qz	228	2	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	228	3	antiserum	antiserum	NN
cord-268139-tgpsu4qz	228	4	is	be	VBZ
cord-268139-tgpsu4qz	228	5	capable	capable	JJ
cord-268139-tgpsu4qz	228	6	of	of	IN
cord-268139-tgpsu4qz	228	7	detecting	detect	VBG
cord-268139-tgpsu4qz	228	8	VUSB1-nsp1	VUSB1-nsp1	NNS
cord-268139-tgpsu4qz	228	9	by	by	IN
cord-268139-tgpsu4qz	228	10	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	228	11	(	(	-LRB-
cord-268139-tgpsu4qz	228	12	Fig	Fig	NNP
cord-268139-tgpsu4qz	228	13	.	.	.
cord-268139-tgpsu4qz	228	14	3A	3A	NNP
cord-268139-tgpsu4qz	228	15	)	)	-RRB-
cord-268139-tgpsu4qz	228	16	,	,	,
cord-268139-tgpsu4qz	228	17	demonstrating	demonstrate	VBG
cord-268139-tgpsu4qz	228	18	that	that	IN
cord-268139-tgpsu4qz	228	19	the	the	DT
cord-268139-tgpsu4qz	228	20	engineered	engineer	VBN
cord-268139-tgpsu4qz	228	21	substitutions	substitution	NNS
cord-268139-tgpsu4qz	228	22	did	do	VBD
cord-268139-tgpsu4qz	228	23	not	not	RB
cord-268139-tgpsu4qz	228	24	abolish	abolish	VB
cord-268139-tgpsu4qz	228	25	the	the	DT
cord-268139-tgpsu4qz	228	26	epitope(s	epitope(s	NNP
cord-268139-tgpsu4qz	228	27	)	)	-RRB-
cord-268139-tgpsu4qz	228	28	.	.	.
cord-268139-tgpsu4qz	229	1	Therefore	therefore	RB
cord-268139-tgpsu4qz	229	2	,	,	,
cord-268139-tgpsu4qz	229	3	the	the	DT
cord-268139-tgpsu4qz	229	4	lack	lack	NN
cord-268139-tgpsu4qz	229	5	of	of	IN
cord-268139-tgpsu4qz	229	6	staining	stain	VBG
cord-268139-tgpsu4qz	229	7	during	during	IN
cord-268139-tgpsu4qz	229	8	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	229	9	assays	assay	NNS
cord-268139-tgpsu4qz	229	10	might	may	MD
cord-268139-tgpsu4qz	229	11	reflect	reflect	VB
cord-268139-tgpsu4qz	229	12	alternative	alternative	JJ
cord-268139-tgpsu4qz	229	13	folding	folding	NN
cord-268139-tgpsu4qz	229	14	of	of	IN
cord-268139-tgpsu4qz	229	15	VUSB1-nsp1	VUSB1-nsp1	NNPS
cord-268139-tgpsu4qz	229	16	.	.	.
cord-268139-tgpsu4qz	230	1	Nonetheless	nonetheless	RB
cord-268139-tgpsu4qz	230	2	,	,	,
cord-268139-tgpsu4qz	230	3	these	these	DT
cord-268139-tgpsu4qz	230	4	results	result	NNS
cord-268139-tgpsu4qz	230	5	suggest	suggest	VBP
cord-268139-tgpsu4qz	230	6	that	that	IN
cord-268139-tgpsu4qz	230	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	230	8	mutant	mutant	JJ
cord-268139-tgpsu4qz	230	9	viruses	virus	NNS
cord-268139-tgpsu4qz	230	10	do	do	VBP
cord-268139-tgpsu4qz	230	11	not	not	RB
cord-268139-tgpsu4qz	230	12	have	have	VB
cord-268139-tgpsu4qz	230	13	defects	defect	NNS
cord-268139-tgpsu4qz	230	14	in	in	IN
cord-268139-tgpsu4qz	230	15	intracellular	intracellular	JJ
cord-268139-tgpsu4qz	230	16	protein	protein	NN
cord-268139-tgpsu4qz	230	17	localization	localization	NN
cord-268139-tgpsu4qz	230	18	or	or	CC
cord-268139-tgpsu4qz	230	19	replication	replication	NN
cord-268139-tgpsu4qz	230	20	complex	complex	JJ
cord-268139-tgpsu4qz	230	21	formation	formation	NN
cord-268139-tgpsu4qz	230	22	.	.	.
cord-268139-tgpsu4qz	231	1	To	to	TO
cord-268139-tgpsu4qz	231	2	investigate	investigate	VB
cord-268139-tgpsu4qz	231	3	whether	whether	IN
cord-268139-tgpsu4qz	231	4	the	the	DT
cord-268139-tgpsu4qz	231	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	231	6	mutant	mutant	JJ
cord-268139-tgpsu4qz	231	7	viruses	virus	NNS
cord-268139-tgpsu4qz	231	8	exhibit	exhibit	VBP
cord-268139-tgpsu4qz	231	9	changes	change	NNS
cord-268139-tgpsu4qz	231	10	in	in	IN
cord-268139-tgpsu4qz	231	11	the	the	DT
cord-268139-tgpsu4qz	231	12	timing	timing	NN
cord-268139-tgpsu4qz	231	13	or	or	CC
cord-268139-tgpsu4qz	231	14	levels	level	NNS
cord-268139-tgpsu4qz	231	15	of	of	IN
cord-268139-tgpsu4qz	231	16	viral	viral	JJ
cord-268139-tgpsu4qz	231	17	RNA	RNA	NNP
cord-268139-tgpsu4qz	231	18	synthesis	synthesis	NN
cord-268139-tgpsu4qz	231	19	,	,	,
cord-268139-tgpsu4qz	231	20	metabolic	metabolic	JJ
cord-268139-tgpsu4qz	231	21	labeling	labeling	NN
cord-268139-tgpsu4qz	231	22	assays	assay	NNS
cord-268139-tgpsu4qz	231	23	were	be	VBD
cord-268139-tgpsu4qz	231	24	performed	perform	VBN
cord-268139-tgpsu4qz	231	25	(	(	-LRB-
cord-268139-tgpsu4qz	231	26	Fig	Fig	NNP
cord-268139-tgpsu4qz	231	27	.	.	.
cord-268139-tgpsu4qz	231	28	6	6	CD
cord-268139-tgpsu4qz	231	29	)	)	-RRB-
cord-268139-tgpsu4qz	231	30	.	.	.
cord-268139-tgpsu4qz	232	1	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	233	1	Fig	Fig	NNP
cord-268139-tgpsu4qz	233	2	.	.	.
cord-268139-tgpsu4qz	234	1	6	6	LS
cord-268139-tgpsu4qz	234	2	.	.	.
cord-268139-tgpsu4qz	235	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	235	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	235	3	viral	viral	JJ
cord-268139-tgpsu4qz	235	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	235	5	levels	level	NNS
cord-268139-tgpsu4qz	235	6	.	.	.
cord-268139-tgpsu4qz	236	1	(	(	-LRB-
cord-268139-tgpsu4qz	236	2	A	a	NN
cord-268139-tgpsu4qz	236	3	)	)	-RRB-
cord-268139-tgpsu4qz	237	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	237	2	mutant	mutant	JJ
cord-268139-tgpsu4qz	237	3	viral	viral	JJ
cord-268139-tgpsu4qz	237	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	237	5	levels	level	NNS
cord-268139-tgpsu4qz	237	6	represented	represent	VBN
cord-268139-tgpsu4qz	237	7	as	as	IN
cord-268139-tgpsu4qz	237	8	percent	percent	NN
cord-268139-tgpsu4qz	237	9	of	of	IN
cord-268139-tgpsu4qz	237	10	icwt	icwt	NNP
cord-268139-tgpsu4qz	237	11	.	.	.
cord-268139-tgpsu4qz	238	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	238	2	cells	cell	NNS
cord-268139-tgpsu4qz	238	3	were	be	VBD
cord-268139-tgpsu4qz	238	4	infected	infect	VBN
cord-268139-tgpsu4qz	238	5	at	at	IN
cord-268139-tgpsu4qz	238	6	an	an	DT
cord-268139-tgpsu4qz	238	7	MOI	MOI	NNP
cord-268139-tgpsu4qz	238	8	of	of	IN
cord-268139-tgpsu4qz	238	9	5	5	CD
cord-268139-tgpsu4qz	238	10	with	with	IN
cord-268139-tgpsu4qz	238	11	icwt	icwt	NNP
cord-268139-tgpsu4qz	238	12	or	or	CC
cord-268139-tgpsu4qz	238	13	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	238	14	mutant	mutant	JJ
cord-268139-tgpsu4qz	238	15	viruses	virus	NNS
cord-268139-tgpsu4qz	238	16	.	.	.
cord-268139-tgpsu4qz	239	1	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	239	2	D	D	NNP
cord-268139-tgpsu4qz	239	3	was	be	VBD
cord-268139-tgpsu4qz	239	4	added	add	VBN
cord-268139-tgpsu4qz	239	5	to	to	IN
cord-268139-tgpsu4qz	239	6	a	a	DT
cord-268139-tgpsu4qz	239	7	final	final	JJ
cord-268139-tgpsu4qz	239	8	concentration	concentration	NN
cord-268139-tgpsu4qz	239	9	of	of	IN
cord-268139-tgpsu4qz	239	10	20	20	CD
cord-268139-tgpsu4qz	239	11	Ag	Ag	NNP
cord-268139-tgpsu4qz	239	12	/	/	SYM
cord-268139-tgpsu4qz	239	13	ml	ml	NN
cord-268139-tgpsu4qz	239	14	30	30	CD
cord-268139-tgpsu4qz	239	15	min	min	NN
cord-268139-tgpsu4qz	239	16	prior	prior	RB
cord-268139-tgpsu4qz	239	17	to	to	IN
cord-268139-tgpsu4qz	239	18	the	the	DT
cord-268139-tgpsu4qz	239	19	addition	addition	NN
cord-268139-tgpsu4qz	239	20	of	of	IN
cord-268139-tgpsu4qz	239	21	[	[	-LRB-
cord-268139-tgpsu4qz	239	22	3	3	CD
cord-268139-tgpsu4qz	239	23	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	239	24	.	.	.
cord-268139-tgpsu4qz	240	1	Viral	Viral	NNP
cord-268139-tgpsu4qz	240	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	240	3	was	be	VBD
cord-268139-tgpsu4qz	240	4	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	240	5	from	from	IN
cord-268139-tgpsu4qz	240	6	5	5	CD
cord-268139-tgpsu4qz	240	7	-9	-9	NN
cord-268139-tgpsu4qz	240	8	h	h	NN
cord-268139-tgpsu4qz	240	9	p.i	p.i	NNP
cord-268139-tgpsu4qz	240	10	.	.	NNP
cord-268139-tgpsu4qz	240	11	and	and	CC
cord-268139-tgpsu4qz	240	12	then	then	RB
cord-268139-tgpsu4qz	240	13	precipitated	precipitate	VBD
cord-268139-tgpsu4qz	240	14	from	from	IN
cord-268139-tgpsu4qz	240	15	equal	equal	JJ
cord-268139-tgpsu4qz	240	16	volumes	volume	NNS
cord-268139-tgpsu4qz	240	17	of	of	IN
cord-268139-tgpsu4qz	240	18	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	240	19	lysates	lysate	NNS
cord-268139-tgpsu4qz	240	20	in	in	IN
cord-268139-tgpsu4qz	240	21	replicate	replicate	NN
cord-268139-tgpsu4qz	240	22	using	use	VBG
cord-268139-tgpsu4qz	240	23	trichloracetic	trichloracetic	JJ
cord-268139-tgpsu4qz	240	24	acid	acid	NN
cord-268139-tgpsu4qz	240	25	.	.	.
cord-268139-tgpsu4qz	241	1	To	to	TO
cord-268139-tgpsu4qz	241	2	quantitate	quantitate	VB
cord-268139-tgpsu4qz	241	3	[	[	-LRB-
cord-268139-tgpsu4qz	241	4	3	3	CD
cord-268139-tgpsu4qz	241	5	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	241	6	incorporation	incorporation	NN
cord-268139-tgpsu4qz	241	7	as	as	IN
cord-268139-tgpsu4qz	241	8	counts	count	NNS
cord-268139-tgpsu4qz	241	9	per	per	IN
cord-268139-tgpsu4qz	241	10	minute	minute	NN
cord-268139-tgpsu4qz	241	11	(	(	-LRB-
cord-268139-tgpsu4qz	241	12	CPM	CPM	NNP
cord-268139-tgpsu4qz	241	13	)	)	-RRB-
cord-268139-tgpsu4qz	241	14	,	,	,
cord-268139-tgpsu4qz	241	15	liquid	liquid	JJ
cord-268139-tgpsu4qz	241	16	scintillation	scintillation	NN
cord-268139-tgpsu4qz	241	17	was	be	VBD
cord-268139-tgpsu4qz	241	18	used	use	VBN
cord-268139-tgpsu4qz	241	19	.	.	.
cord-268139-tgpsu4qz	242	1	For	for	IN
cord-268139-tgpsu4qz	242	2	each	each	DT
cord-268139-tgpsu4qz	242	3	experiment	experiment	NN
cord-268139-tgpsu4qz	242	4	(	(	-LRB-
cord-268139-tgpsu4qz	242	5	n	n	NN
cord-268139-tgpsu4qz	242	6	=	=	SYM
cord-268139-tgpsu4qz	242	7	5	5	CD
cord-268139-tgpsu4qz	242	8	)	)	-RRB-
cord-268139-tgpsu4qz	242	9	,	,	,
cord-268139-tgpsu4qz	242	10	labeled	label	VBD
cord-268139-tgpsu4qz	242	11	viral	viral	JJ
cord-268139-tgpsu4qz	242	12	RNA	RNA	NNP
cord-268139-tgpsu4qz	242	13	levels	level	NNS
cord-268139-tgpsu4qz	242	14	(	(	-LRB-
cord-268139-tgpsu4qz	242	15	CPM	CPM	NNP
cord-268139-tgpsu4qz	242	16	)	)	-RRB-
cord-268139-tgpsu4qz	242	17	for	for	IN
cord-268139-tgpsu4qz	242	18	icwt	icwt	NNP
cord-268139-tgpsu4qz	242	19	were	be	VBD
cord-268139-tgpsu4qz	242	20	set	set	VBN
cord-268139-tgpsu4qz	242	21	to	to	IN
cord-268139-tgpsu4qz	242	22	100	100	CD
cord-268139-tgpsu4qz	242	23	%	%	NN
cord-268139-tgpsu4qz	242	24	,	,	,
cord-268139-tgpsu4qz	242	25	and	and	CC
cord-268139-tgpsu4qz	242	26	nsp1	nsp1	NN
cord-268139-tgpsu4qz	242	27	mutant	mutant	JJ
cord-268139-tgpsu4qz	242	28	viral	viral	JJ
cord-268139-tgpsu4qz	242	29	RNA	RNA	NNP
cord-268139-tgpsu4qz	242	30	was	be	VBD
cord-268139-tgpsu4qz	242	31	calculated	calculate	VBN
cord-268139-tgpsu4qz	242	32	as	as	IN
cord-268139-tgpsu4qz	242	33	a	a	DT
cord-268139-tgpsu4qz	242	34	percentage	percentage	NN
cord-268139-tgpsu4qz	242	35	of	of	IN
cord-268139-tgpsu4qz	242	36	the	the	DT
cord-268139-tgpsu4qz	242	37	icwt	icwt	JJ
cord-268139-tgpsu4qz	242	38	value	value	NN
cord-268139-tgpsu4qz	242	39	.	.	.
cord-268139-tgpsu4qz	243	1	The	the	DT
cord-268139-tgpsu4qz	243	2	bars	bar	NNS
cord-268139-tgpsu4qz	243	3	represent	represent	VBP
cord-268139-tgpsu4qz	243	4	the	the	DT
cord-268139-tgpsu4qz	243	5	average	average	JJ
cord-268139-tgpsu4qz	243	6	percent	percent	NN
cord-268139-tgpsu4qz	243	7	viral	viral	JJ
cord-268139-tgpsu4qz	243	8	RNA	RNA	NNP
cord-268139-tgpsu4qz	243	9	from	from	IN
cord-268139-tgpsu4qz	243	10	all	all	DT
cord-268139-tgpsu4qz	243	11	experiments	experiment	NNS
cord-268139-tgpsu4qz	243	12	,	,	,
cord-268139-tgpsu4qz	243	13	and	and	CC
cord-268139-tgpsu4qz	243	14	lines	line	NNS
cord-268139-tgpsu4qz	243	15	indicate	indicate	VBP
cord-268139-tgpsu4qz	243	16	standard	standard	JJ
cord-268139-tgpsu4qz	243	17	error	error	NN
cord-268139-tgpsu4qz	243	18	.	.	.
cord-268139-tgpsu4qz	244	1	Statistical	statistical	JJ
cord-268139-tgpsu4qz	244	2	analysis	analysis	NN
cord-268139-tgpsu4qz	244	3	software	software	NN
cord-268139-tgpsu4qz	244	4	was	be	VBD
cord-268139-tgpsu4qz	244	5	used	use	VBN
cord-268139-tgpsu4qz	244	6	to	to	TO
cord-268139-tgpsu4qz	244	7	determine	determine	VB
cord-268139-tgpsu4qz	244	8	P	p	NN
cord-268139-tgpsu4qz	244	9	values	value	NNS
cord-268139-tgpsu4qz	244	10	using	use	VBG
cord-268139-tgpsu4qz	244	11	a	a	DT
cord-268139-tgpsu4qz	244	12	one	one	CD
cord-268139-tgpsu4qz	244	13	-	-	HYPH
cord-268139-tgpsu4qz	244	14	sample	sample	NN
cord-268139-tgpsu4qz	244	15	t	t	NN
cord-268139-tgpsu4qz	244	16	test	test	NN
cord-268139-tgpsu4qz	244	17	.	.	.
cord-268139-tgpsu4qz	245	1	Asterisks	asterisk	NNS
cord-268139-tgpsu4qz	245	2	(	(	-LRB-
cord-268139-tgpsu4qz	245	3	*	*	NFP
cord-268139-tgpsu4qz	245	4	)	)	-RRB-
cord-268139-tgpsu4qz	245	5	indicate	indicate	VBP
cord-268139-tgpsu4qz	245	6	P	p	NN
cord-268139-tgpsu4qz	245	7	values	value	NNS
cord-268139-tgpsu4qz	245	8	0.05	0.05	CD
cord-268139-tgpsu4qz	245	9	.	.	.
cord-268139-tgpsu4qz	246	1	(	(	-LRB-
cord-268139-tgpsu4qz	246	2	B	b	NN
cord-268139-tgpsu4qz	246	3	)	)	-RRB-
cord-268139-tgpsu4qz	247	1	Gel	gel	NN
cord-268139-tgpsu4qz	247	2	analysis	analysis	NN
cord-268139-tgpsu4qz	247	3	of	of	IN
cord-268139-tgpsu4qz	247	4	viral	viral	JJ
cord-268139-tgpsu4qz	247	5	RNA	RNA	NNP
cord-268139-tgpsu4qz	247	6	.	.	.
cord-268139-tgpsu4qz	248	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	248	2	cells	cell	NNS
cord-268139-tgpsu4qz	248	3	were	be	VBD
cord-268139-tgpsu4qz	248	4	mock	mock	JJ
cord-268139-tgpsu4qz	248	5	-	-	HYPH
cord-268139-tgpsu4qz	248	6	infected	infect	VBN
cord-268139-tgpsu4qz	248	7	or	or	CC
cord-268139-tgpsu4qz	248	8	infected	infect	VBN
cord-268139-tgpsu4qz	248	9	with	with	IN
cord-268139-tgpsu4qz	248	10	icwt	icwt	NNP
cord-268139-tgpsu4qz	248	11	or	or	CC
cord-268139-tgpsu4qz	248	12	the	the	DT
cord-268139-tgpsu4qz	248	13	indicated	indicate	VBN
cord-268139-tgpsu4qz	248	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	248	15	mutants	mutant	NNS
cord-268139-tgpsu4qz	248	16	.	.	.
cord-268139-tgpsu4qz	249	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	249	2	was	be	VBD
cord-268139-tgpsu4qz	249	3	labeled	label	VBN
cord-268139-tgpsu4qz	249	4	as	as	IN
cord-268139-tgpsu4qz	249	5	in	in	IN
cord-268139-tgpsu4qz	249	6	panel	panel	NN
cord-268139-tgpsu4qz	249	7	(	(	-LRB-
cord-268139-tgpsu4qz	249	8	A	a	NN
cord-268139-tgpsu4qz	249	9	)	)	-RRB-
cord-268139-tgpsu4qz	249	10	above	above	RB
cord-268139-tgpsu4qz	249	11	,	,	,
cord-268139-tgpsu4qz	249	12	and	and	CC
cord-268139-tgpsu4qz	249	13	cells	cell	NNS
cord-268139-tgpsu4qz	249	14	were	be	VBD
cord-268139-tgpsu4qz	249	15	lysed	lyse	VBN
cord-268139-tgpsu4qz	249	16	using	use	VBG
cord-268139-tgpsu4qz	249	17	Trizol	Trizol	NNP
cord-268139-tgpsu4qz	249	18	.	.	.
cord-268139-tgpsu4qz	250	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	250	2	was	be	VBD
cord-268139-tgpsu4qz	250	3	isolated	isolate	VBN
cord-268139-tgpsu4qz	250	4	from	from	IN
cord-268139-tgpsu4qz	250	5	cell	cell	NN
cord-268139-tgpsu4qz	250	6	lysates	lysate	NNS
cord-268139-tgpsu4qz	250	7	and	and	CC
cord-268139-tgpsu4qz	250	8	normalized	normalize	VBD
cord-268139-tgpsu4qz	250	9	so	so	IN
cord-268139-tgpsu4qz	250	10	as	as	IN
cord-268139-tgpsu4qz	250	11	to	to	TO
cord-268139-tgpsu4qz	250	12	electrophorese	electrophorese	NNP
cord-268139-tgpsu4qz	250	13	the	the	DT
cord-268139-tgpsu4qz	250	14	same	same	JJ
cord-268139-tgpsu4qz	250	15	amount	amount	NN
cord-268139-tgpsu4qz	250	16	of	of	IN
cord-268139-tgpsu4qz	250	17	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	250	18	viral	viral	JJ
cord-268139-tgpsu4qz	250	19	RNA	RNA	NNP
cord-268139-tgpsu4qz	250	20	for	for	IN
cord-268139-tgpsu4qz	250	21	each	each	DT
cord-268139-tgpsu4qz	250	22	mutant	mutant	NN
cord-268139-tgpsu4qz	250	23	(	(	-LRB-
cord-268139-tgpsu4qz	250	24	approximately	approximately	RB
cord-268139-tgpsu4qz	250	25	400,000	400,000	CD
cord-268139-tgpsu4qz	250	26	CPM	CPM	NNS
cord-268139-tgpsu4qz	250	27	from	from	IN
cord-268139-tgpsu4qz	250	28	a	a	DT
cord-268139-tgpsu4qz	250	29	maximum	maximum	NN
cord-268139-tgpsu4qz	250	30	of	of	IN
cord-268139-tgpsu4qz	250	31	10	10	CD
cord-268139-tgpsu4qz	250	32	6	6	CD
cord-268139-tgpsu4qz	250	33	cells	cell	NNS
cord-268139-tgpsu4qz	250	34	)	)	-RRB-
cord-268139-tgpsu4qz	250	35	.	.	.
cord-268139-tgpsu4qz	251	1	For	for	IN
cord-268139-tgpsu4qz	251	2	the	the	DT
cord-268139-tgpsu4qz	251	3	mock	mock	NN
cord-268139-tgpsu4qz	251	4	-	-	HYPH
cord-268139-tgpsu4qz	251	5	infected	infect	VBN
cord-268139-tgpsu4qz	251	6	control	control	NN
cord-268139-tgpsu4qz	251	7	(	(	-LRB-
cord-268139-tgpsu4qz	251	8	m	m	NNP
cord-268139-tgpsu4qz	251	9	)	)	-RRB-
cord-268139-tgpsu4qz	251	10	,	,	,
cord-268139-tgpsu4qz	251	11	RNA	RNA	NNP
cord-268139-tgpsu4qz	251	12	from	from	IN
cord-268139-tgpsu4qz	251	13	10	10	CD
cord-268139-tgpsu4qz	251	14	6	6	CD
cord-268139-tgpsu4qz	251	15	cells	cell	NNS
cord-268139-tgpsu4qz	251	16	was	be	VBD
cord-268139-tgpsu4qz	251	17	used	use	VBN
cord-268139-tgpsu4qz	251	18	.	.	.
cord-268139-tgpsu4qz	252	1	The	the	DT
cord-268139-tgpsu4qz	252	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	252	3	was	be	VBD
cord-268139-tgpsu4qz	252	4	separated	separate	VBN
cord-268139-tgpsu4qz	252	5	in	in	IN
cord-268139-tgpsu4qz	252	6	an	an	DT
cord-268139-tgpsu4qz	252	7	0.8	0.8	CD
cord-268139-tgpsu4qz	252	8	%	%	NN
cord-268139-tgpsu4qz	252	9	formaldehyde	formaldehyde	NN
cord-268139-tgpsu4qz	252	10	/	/	SYM
cord-268139-tgpsu4qz	252	11	agarose	agarose	VBN
cord-268139-tgpsu4qz	252	12	gel	gel	NN
cord-268139-tgpsu4qz	252	13	,	,	,
cord-268139-tgpsu4qz	252	14	and	and	CC
cord-268139-tgpsu4qz	252	15	individual	individual	JJ
cord-268139-tgpsu4qz	252	16	RNA	RNA	NNP
cord-268139-tgpsu4qz	252	17	species	specie	NNS
cord-268139-tgpsu4qz	252	18	were	be	VBD
cord-268139-tgpsu4qz	252	19	visualized	visualize	VBN
cord-268139-tgpsu4qz	252	20	following	follow	VBG
cord-268139-tgpsu4qz	252	21	fluorography	fluorography	NN
cord-268139-tgpsu4qz	252	22	.	.	.
cord-268139-tgpsu4qz	253	1	The	the	DT
cord-268139-tgpsu4qz	253	2	image	image	NN
cord-268139-tgpsu4qz	253	3	is	be	VBZ
cord-268139-tgpsu4qz	253	4	from	from	IN
cord-268139-tgpsu4qz	253	5	a	a	DT
cord-268139-tgpsu4qz	253	6	2-day	2-day	JJ
cord-268139-tgpsu4qz	253	7	exposure	exposure	NN
cord-268139-tgpsu4qz	253	8	of	of	IN
cord-268139-tgpsu4qz	253	9	the	the	DT
cord-268139-tgpsu4qz	253	10	gel	gel	NN
cord-268139-tgpsu4qz	253	11	to	to	TO
cord-268139-tgpsu4qz	253	12	film	film	VB
cord-268139-tgpsu4qz	253	13	.	.	.
cord-268139-tgpsu4qz	254	1	Individual	individual	JJ
cord-268139-tgpsu4qz	254	2	viral	viral	JJ
cord-268139-tgpsu4qz	254	3	RNA	RNA	NNP
cord-268139-tgpsu4qz	254	4	species	specie	NNS
cord-268139-tgpsu4qz	254	5	are	be	VBP
cord-268139-tgpsu4qz	254	6	numbered	number	VBN
cord-268139-tgpsu4qz	254	7	to	to	IN
cord-268139-tgpsu4qz	254	8	the	the	DT
cord-268139-tgpsu4qz	254	9	right	right	NN
cord-268139-tgpsu4qz	254	10	of	of	IN
cord-268139-tgpsu4qz	254	11	the	the	DT
cord-268139-tgpsu4qz	254	12	fluorogram	fluorogram	NNP
cord-268139-tgpsu4qz	255	1	(	(	-LRB-
cord-268139-tgpsu4qz	255	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	255	3	1	1	CD
cord-268139-tgpsu4qz	255	4	=	=	SYM
cord-268139-tgpsu4qz	255	5	genome	genome	NN
cord-268139-tgpsu4qz	255	6	and	and	CC
cord-268139-tgpsu4qz	255	7	RNA	RNA	NNP
cord-268139-tgpsu4qz	255	8	2	2	CD
cord-268139-tgpsu4qz	255	9	-7	-7	NNP
cord-268139-tgpsu4qz	255	10	=	=	SYM
cord-268139-tgpsu4qz	255	11	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	255	12	RNAs	RNAs	NNPS
cord-268139-tgpsu4qz	255	13	)	)	-RRB-
cord-268139-tgpsu4qz	255	14	.	.	.
cord-268139-tgpsu4qz	256	1	The	the	DT
cord-268139-tgpsu4qz	256	2	asterisk	asterisk	NN
cord-268139-tgpsu4qz	256	3	(	(	-LRB-
cord-268139-tgpsu4qz	256	4	*	*	NFP
cord-268139-tgpsu4qz	256	5	)	)	-RRB-
cord-268139-tgpsu4qz	256	6	indicates	indicate	VBZ
cord-268139-tgpsu4qz	256	7	a	a	DT
cord-268139-tgpsu4qz	256	8	unique	unique	JJ
cord-268139-tgpsu4qz	256	9	RNA	RNA	NNP
cord-268139-tgpsu4qz	256	10	band	band	NN
cord-268139-tgpsu4qz	256	11	detected	detect	VBN
cord-268139-tgpsu4qz	256	12	in	in	IN
cord-268139-tgpsu4qz	256	13	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	256	14	virus	virus	NN
cord-268139-tgpsu4qz	256	15	-	-	HYPH
cord-268139-tgpsu4qz	256	16	infected	infect	VBN
cord-268139-tgpsu4qz	256	17	cells	cell	NNS
cord-268139-tgpsu4qz	256	18	.	.	.
cord-268139-tgpsu4qz	257	1	cells	cell	NNS
cord-268139-tgpsu4qz	257	2	were	be	VBD
cord-268139-tgpsu4qz	257	3	infected	infect	VBN
cord-268139-tgpsu4qz	257	4	with	with	IN
cord-268139-tgpsu4qz	257	5	icwt	icwt	NNP
cord-268139-tgpsu4qz	257	6	or	or	CC
cord-268139-tgpsu4qz	257	7	nsp1	nsp1	CD
cord-268139-tgpsu4qz	257	8	mutants	mutant	NNS
cord-268139-tgpsu4qz	257	9	(	(	-LRB-
cord-268139-tgpsu4qz	257	10	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	257	11	,	,	,
cord-268139-tgpsu4qz	257	12	VUSB2	vusb2	NN
cord-268139-tgpsu4qz	257	13	,	,	,
cord-268139-tgpsu4qz	257	14	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	257	15	,	,	,
cord-268139-tgpsu4qz	257	16	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	257	17	,	,	,
cord-268139-tgpsu4qz	257	18	VUSB7	VUSB7	NNP
cord-268139-tgpsu4qz	257	19	,	,	,
cord-268139-tgpsu4qz	257	20	or	or	CC
cord-268139-tgpsu4qz	257	21	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	257	22	)	)	-RRB-
cord-268139-tgpsu4qz	257	23	.	.	.
cord-268139-tgpsu4qz	258	1	Viral	viral	JJ
cord-268139-tgpsu4qz	258	2	RNAs	rna	NNS
cord-268139-tgpsu4qz	258	3	were	be	VBD
cord-268139-tgpsu4qz	258	4	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	258	5	from	from	IN
cord-268139-tgpsu4qz	258	6	1	1	CD
cord-268139-tgpsu4qz	258	7	-13	-13	CD
cord-268139-tgpsu4qz	258	8	h	h	NN
cord-268139-tgpsu4qz	258	9	p.i	p.i	NNP
cord-268139-tgpsu4qz	258	10	.	.	.
cord-268139-tgpsu4qz	259	1	at	at	IN
cord-268139-tgpsu4qz	259	2	intervals	interval	NNS
cord-268139-tgpsu4qz	259	3	using	use	VBG
cord-268139-tgpsu4qz	259	4	100	100	CD
cord-268139-tgpsu4qz	259	5	ACi	ACi	NNS
cord-268139-tgpsu4qz	259	6	/	/	SYM
cord-268139-tgpsu4qz	259	7	ml	ml	NNS
cord-268139-tgpsu4qz	259	8	of	of	IN
cord-268139-tgpsu4qz	259	9	[	[	-LRB-
cord-268139-tgpsu4qz	259	10	3	3	CD
cord-268139-tgpsu4qz	259	11	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	259	12	in	in	IN
cord-268139-tgpsu4qz	259	13	the	the	DT
cord-268139-tgpsu4qz	259	14	presence	presence	NN
cord-268139-tgpsu4qz	259	15	of	of	IN
cord-268139-tgpsu4qz	259	16	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	259	17	D	D	NNP
cord-268139-tgpsu4qz	259	18	,	,	,
cord-268139-tgpsu4qz	259	19	a	a	DT
cord-268139-tgpsu4qz	259	20	drug	drug	NN
cord-268139-tgpsu4qz	259	21	that	that	WDT
cord-268139-tgpsu4qz	259	22	inhibits	inhibit	VBZ
cord-268139-tgpsu4qz	259	23	DNA	dna	NN
cord-268139-tgpsu4qz	259	24	-	-	HYPH
cord-268139-tgpsu4qz	259	25	dependent	dependent	JJ
cord-268139-tgpsu4qz	259	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	259	27	synthesis	synthesis	NN
cord-268139-tgpsu4qz	259	28	.	.	.
cord-268139-tgpsu4qz	260	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	260	2	was	be	VBD
cord-268139-tgpsu4qz	260	3	precipitated	precipitate	VBN
cord-268139-tgpsu4qz	260	4	from	from	IN
cord-268139-tgpsu4qz	260	5	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	260	6	lysates	lysate	NNS
cord-268139-tgpsu4qz	260	7	using	use	VBG
cord-268139-tgpsu4qz	260	8	trichloroacetic	trichloroacetic	JJ
cord-268139-tgpsu4qz	260	9	acid	acid	NN
cord-268139-tgpsu4qz	260	10	,	,	,
cord-268139-tgpsu4qz	260	11	and	and	CC
cord-268139-tgpsu4qz	260	12	3	3	CD
cord-268139-tgpsu4qz	260	13	H	h	NN
cord-268139-tgpsu4qz	260	14	levels	level	NNS
cord-268139-tgpsu4qz	260	15	were	be	VBD
cord-268139-tgpsu4qz	260	16	quantitated	quantitate	VBN
cord-268139-tgpsu4qz	260	17	using	use	VBG
cord-268139-tgpsu4qz	260	18	liquid	liquid	JJ
cord-268139-tgpsu4qz	260	19	scintillation	scintillation	NN
cord-268139-tgpsu4qz	260	20	.	.	.
cord-268139-tgpsu4qz	261	1	The	the	DT
cord-268139-tgpsu4qz	261	2	timing	timing	NN
cord-268139-tgpsu4qz	261	3	of	of	IN
cord-268139-tgpsu4qz	261	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	261	5	synthesis	synthesis	NN
cord-268139-tgpsu4qz	261	6	for	for	IN
cord-268139-tgpsu4qz	261	7	the	the	DT
cord-268139-tgpsu4qz	261	8	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	261	9	mutants	mutant	NNS
cord-268139-tgpsu4qz	261	10	was	be	VBD
cord-268139-tgpsu4qz	261	11	indistinguishable	indistinguishable	JJ
cord-268139-tgpsu4qz	261	12	from	from	IN
cord-268139-tgpsu4qz	261	13	icwt	icwt	NNP
cord-268139-tgpsu4qz	261	14	,	,	,
cord-268139-tgpsu4qz	261	15	with	with	IN
cord-268139-tgpsu4qz	261	16	all	all	DT
cord-268139-tgpsu4qz	261	17	viruses	virus	NNS
cord-268139-tgpsu4qz	261	18	exhibiting	exhibit	VBG
cord-268139-tgpsu4qz	261	19	maximal	maximal	JJ
cord-268139-tgpsu4qz	261	20	[	[	-LRB-
cord-268139-tgpsu4qz	261	21	3	3	CD
cord-268139-tgpsu4qz	261	22	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	261	23	incorporation	incorporation	NN
cord-268139-tgpsu4qz	261	24	when	when	WRB
cord-268139-tgpsu4qz	261	25	cells	cell	NNS
cord-268139-tgpsu4qz	261	26	were	be	VBD
cord-268139-tgpsu4qz	261	27	labeled	label	VBN
cord-268139-tgpsu4qz	261	28	between	between	IN
cord-268139-tgpsu4qz	261	29	5	5	CD
cord-268139-tgpsu4qz	261	30	-9	-9	NN
cord-268139-tgpsu4qz	261	31	h	h	NN
cord-268139-tgpsu4qz	261	32	p.i	p.i	NNP
cord-268139-tgpsu4qz	261	33	.	.	.
cord-268139-tgpsu4qz	262	1	(	(	-LRB-
cord-268139-tgpsu4qz	262	2	data	datum	NNS
cord-268139-tgpsu4qz	262	3	not	not	RB
cord-268139-tgpsu4qz	262	4	shown	show	VBN
cord-268139-tgpsu4qz	262	5	)	)	-RRB-
cord-268139-tgpsu4qz	262	6	.	.	.
cord-268139-tgpsu4qz	263	1	However	however	RB
cord-268139-tgpsu4qz	263	2	,	,	,
cord-268139-tgpsu4qz	263	3	the	the	DT
cord-268139-tgpsu4qz	263	4	total	total	JJ
cord-268139-tgpsu4qz	263	5	levels	level	NNS
cord-268139-tgpsu4qz	263	6	of	of	IN
cord-268139-tgpsu4qz	263	7	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	263	8	viral	viral	JJ
cord-268139-tgpsu4qz	263	9	RNA	RNA	NNP
cord-268139-tgpsu4qz	263	10	differed	differ	VBD
cord-268139-tgpsu4qz	263	11	among	among	IN
cord-268139-tgpsu4qz	263	12	the	the	DT
cord-268139-tgpsu4qz	263	13	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	263	14	mutants	mutant	NNS
cord-268139-tgpsu4qz	263	15	when	when	WRB
cord-268139-tgpsu4qz	263	16	compared	compare	VBN
cord-268139-tgpsu4qz	263	17	with	with	IN
cord-268139-tgpsu4qz	263	18	icwt	icwt	NNP
cord-268139-tgpsu4qz	263	19	.	.	.
cord-268139-tgpsu4qz	264	1	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	264	2	and	and	CC
cord-268139-tgpsu4qz	264	3	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	264	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	264	5	levels	level	NNS
cord-268139-tgpsu4qz	264	6	were	be	VBD
cord-268139-tgpsu4qz	264	7	only	only	RB
cord-268139-tgpsu4qz	264	8	73	73	CD
cord-268139-tgpsu4qz	264	9	%	%	NN
cord-268139-tgpsu4qz	264	10	and	and	CC
cord-268139-tgpsu4qz	264	11	79	79	CD
cord-268139-tgpsu4qz	264	12	%	%	NN
cord-268139-tgpsu4qz	264	13	of	of	IN
cord-268139-tgpsu4qz	264	14	icwt	icwt	NNP
cord-268139-tgpsu4qz	264	15	,	,	,
cord-268139-tgpsu4qz	264	16	respectively	respectively	RB
cord-268139-tgpsu4qz	264	17	,	,	,
cord-268139-tgpsu4qz	264	18	whereas	whereas	IN
cord-268139-tgpsu4qz	264	19	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	264	20	RNA	RNA	NNP
cord-268139-tgpsu4qz	264	21	levels	level	NNS
cord-268139-tgpsu4qz	264	22	were	be	VBD
cord-268139-tgpsu4qz	264	23	129	129	CD
cord-268139-tgpsu4qz	264	24	%	%	NN
cord-268139-tgpsu4qz	264	25	if	if	IN
cord-268139-tgpsu4qz	264	26	icwt	icwt	NNP
cord-268139-tgpsu4qz	264	27	(	(	-LRB-
cord-268139-tgpsu4qz	264	28	Fig	Fig	NNP
cord-268139-tgpsu4qz	264	29	.	.	.
cord-268139-tgpsu4qz	264	30	6A	6a	CD
cord-268139-tgpsu4qz	264	31	)	)	-RRB-
cord-268139-tgpsu4qz	264	32	.	.	.
cord-268139-tgpsu4qz	265	1	These	these	DT
cord-268139-tgpsu4qz	265	2	data	datum	NNS
cord-268139-tgpsu4qz	265	3	suggest	suggest	VBP
cord-268139-tgpsu4qz	265	4	that	that	IN
cord-268139-tgpsu4qz	265	5	nsp1	nsp1	PRP
cord-268139-tgpsu4qz	265	6	may	may	MD
cord-268139-tgpsu4qz	265	7	function	function	VB
cord-268139-tgpsu4qz	265	8	at	at	IN
cord-268139-tgpsu4qz	265	9	the	the	DT
cord-268139-tgpsu4qz	265	10	replication	replication	NN
cord-268139-tgpsu4qz	265	11	complex	complex	NN
cord-268139-tgpsu4qz	265	12	in	in	IN
cord-268139-tgpsu4qz	265	13	the	the	DT
cord-268139-tgpsu4qz	265	14	synthesis	synthesis	NN
cord-268139-tgpsu4qz	265	15	of	of	IN
cord-268139-tgpsu4qz	265	16	viral	viral	JJ
cord-268139-tgpsu4qz	265	17	RNA	RNA	NNP
cord-268139-tgpsu4qz	265	18	.	.	.
cord-268139-tgpsu4qz	266	1	To	to	TO
cord-268139-tgpsu4qz	266	2	determine	determine	VB
cord-268139-tgpsu4qz	266	3	whether	whether	IN
cord-268139-tgpsu4qz	266	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	266	5	mutants	mutant	NNS
cord-268139-tgpsu4qz	266	6	have	have	VBP
cord-268139-tgpsu4qz	266	7	specific	specific	JJ
cord-268139-tgpsu4qz	266	8	defects	defect	NNS
cord-268139-tgpsu4qz	266	9	in	in	IN
cord-268139-tgpsu4qz	266	10	genome	genome	NN
cord-268139-tgpsu4qz	266	11	replication	replication	NN
cord-268139-tgpsu4qz	266	12	or	or	CC
cord-268139-tgpsu4qz	266	13	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	266	14	RNA	RNA	NNP
cord-268139-tgpsu4qz	266	15	synthesis	synthesis	NN
cord-268139-tgpsu4qz	266	16	,	,	,
cord-268139-tgpsu4qz	266	17	radiolabeled	radiolabele	VBD
cord-268139-tgpsu4qz	266	18	viral	viral	JJ
cord-268139-tgpsu4qz	266	19	RNA	RNA	NNP
cord-268139-tgpsu4qz	266	20	was	be	VBD
cord-268139-tgpsu4qz	266	21	analyzed	analyze	VBN
cord-268139-tgpsu4qz	266	22	by	by	IN
cord-268139-tgpsu4qz	266	23	gel	gel	NN
cord-268139-tgpsu4qz	266	24	electrophoresis	electrophoresis	NN
cord-268139-tgpsu4qz	266	25	(	(	-LRB-
cord-268139-tgpsu4qz	266	26	Fig	Fig	NNP
cord-268139-tgpsu4qz	266	27	.	.	.
cord-268139-tgpsu4qz	266	28	6B	6b	CD
cord-268139-tgpsu4qz	266	29	)	)	-RRB-
cord-268139-tgpsu4qz	266	30	.	.	.
cord-268139-tgpsu4qz	267	1	DBT-9	dbt-9	JJ
cord-268139-tgpsu4qz	267	2	cells	cell	NNS
cord-268139-tgpsu4qz	267	3	were	be	VBD
cord-268139-tgpsu4qz	267	4	either	either	DT
cord-268139-tgpsu4qz	267	5	mock	mock	NN
cord-268139-tgpsu4qz	267	6	-	-	HYPH
cord-268139-tgpsu4qz	267	7	infected	infect	VBN
cord-268139-tgpsu4qz	267	8	or	or	CC
cord-268139-tgpsu4qz	267	9	infected	infect	VBN
cord-268139-tgpsu4qz	267	10	with	with	IN
cord-268139-tgpsu4qz	267	11	icwt	icwt	NNP
cord-268139-tgpsu4qz	267	12	or	or	CC
cord-268139-tgpsu4qz	267	13	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	267	14	mutant	mutant	JJ
cord-268139-tgpsu4qz	267	15	viruses	virus	NNS
cord-268139-tgpsu4qz	267	16	,	,	,
cord-268139-tgpsu4qz	267	17	and	and	CC
cord-268139-tgpsu4qz	267	18	RNA	RNA	NNP
cord-268139-tgpsu4qz	267	19	was	be	VBD
cord-268139-tgpsu4qz	267	20	labeled	label	VBN
cord-268139-tgpsu4qz	267	21	in	in	IN
cord-268139-tgpsu4qz	267	22	the	the	DT
cord-268139-tgpsu4qz	267	23	presence	presence	NN
cord-268139-tgpsu4qz	267	24	of	of	IN
cord-268139-tgpsu4qz	267	25	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	267	26	D	D	NNP
cord-268139-tgpsu4qz	267	27	from	from	IN
cord-268139-tgpsu4qz	267	28	5	5	CD
cord-268139-tgpsu4qz	267	29	-9	-9	NN
cord-268139-tgpsu4qz	267	30	h	h	NN
cord-268139-tgpsu4qz	267	31	p.i	p.i	NNP
cord-268139-tgpsu4qz	267	32	.	.	.
cord-268139-tgpsu4qz	267	33	using	use	VBG
cord-268139-tgpsu4qz	267	34	[	[	-LRB-
cord-268139-tgpsu4qz	267	35	3	3	CD
cord-268139-tgpsu4qz	267	36	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	267	37	.	.	.
cord-268139-tgpsu4qz	268	1	Total	total	JJ
cord-268139-tgpsu4qz	268	2	viral	viral	JJ
cord-268139-tgpsu4qz	268	3	and	and	CC
cord-268139-tgpsu4qz	268	4	cellular	cellular	JJ
cord-268139-tgpsu4qz	268	5	RNA	RNA	NNP
cord-268139-tgpsu4qz	268	6	was	be	VBD
cord-268139-tgpsu4qz	268	7	extracted	extract	VBN
cord-268139-tgpsu4qz	268	8	from	from	IN
cord-268139-tgpsu4qz	268	9	cell	cell	NN
cord-268139-tgpsu4qz	268	10	lysates	lysate	NNS
cord-268139-tgpsu4qz	268	11	,	,	,
cord-268139-tgpsu4qz	268	12	and	and	CC
cord-268139-tgpsu4qz	268	13	radioactivity	radioactivity	NN
cord-268139-tgpsu4qz	268	14	in	in	IN
cord-268139-tgpsu4qz	268	15	each	each	DT
cord-268139-tgpsu4qz	268	16	sample	sample	NN
cord-268139-tgpsu4qz	268	17	was	be	VBD
cord-268139-tgpsu4qz	268	18	quantitated	quantitate	VBN
cord-268139-tgpsu4qz	268	19	using	use	VBG
cord-268139-tgpsu4qz	268	20	liquid	liquid	JJ
cord-268139-tgpsu4qz	268	21	scintillation	scintillation	NN
cord-268139-tgpsu4qz	268	22	.	.	.
cord-268139-tgpsu4qz	269	1	Equal	equal	JJ
cord-268139-tgpsu4qz	269	2	amounts	amount	NNS
cord-268139-tgpsu4qz	269	3	of	of	IN
cord-268139-tgpsu4qz	269	4	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	269	5	viral	viral	JJ
cord-268139-tgpsu4qz	269	6	RNA	RNA	NNP
cord-268139-tgpsu4qz	269	7	(	(	-LRB-
cord-268139-tgpsu4qz	269	8	equal	equal	JJ
cord-268139-tgpsu4qz	269	9	CPM	CPM	NNP
cord-268139-tgpsu4qz	269	10	)	)	-RRB-
cord-268139-tgpsu4qz	269	11	were	be	VBD
cord-268139-tgpsu4qz	269	12	separated	separate	VBN
cord-268139-tgpsu4qz	269	13	by	by	IN
cord-268139-tgpsu4qz	269	14	electrophoresis	electrophoresis	NN
cord-268139-tgpsu4qz	269	15	in	in	IN
cord-268139-tgpsu4qz	269	16	formaldehyde	formaldehyde	NN
cord-268139-tgpsu4qz	269	17	/	/	SYM
cord-268139-tgpsu4qz	269	18	agarose	agarose	VBN
cord-268139-tgpsu4qz	269	19	gels	gel	NNS
cord-268139-tgpsu4qz	269	20	and	and	CC
cord-268139-tgpsu4qz	269	21	then	then	RB
cord-268139-tgpsu4qz	269	22	visualized	visualize	VBD
cord-268139-tgpsu4qz	269	23	following	follow	VBG
cord-268139-tgpsu4qz	269	24	fluorography	fluorography	NN
cord-268139-tgpsu4qz	269	25	.	.	.
cord-268139-tgpsu4qz	270	1	All	all	DT
cord-268139-tgpsu4qz	270	2	seven	seven	CD
cord-268139-tgpsu4qz	270	3	species	specie	NNS
cord-268139-tgpsu4qz	270	4	of	of	IN
cord-268139-tgpsu4qz	270	5	MHV	MHV	NNP
cord-268139-tgpsu4qz	270	6	viral	viral	JJ
cord-268139-tgpsu4qz	270	7	RNA	RNA	NNP
cord-268139-tgpsu4qz	270	8	were	be	VBD
cord-268139-tgpsu4qz	270	9	detected	detect	VBN
cord-268139-tgpsu4qz	270	10	with	with	IN
cord-268139-tgpsu4qz	270	11	the	the	DT
cord-268139-tgpsu4qz	270	12	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	270	13	mutant	mutant	JJ
cord-268139-tgpsu4qz	270	14	viruses	virus	NNS
cord-268139-tgpsu4qz	270	15	and	and	CC
cord-268139-tgpsu4qz	270	16	icwt	icwt	NNP
cord-268139-tgpsu4qz	270	17	.	.	.
cord-268139-tgpsu4qz	271	1	Interestingly	interestingly	RB
cord-268139-tgpsu4qz	271	2	,	,	,
cord-268139-tgpsu4qz	271	3	with	with	IN
cord-268139-tgpsu4qz	271	4	the	the	DT
cord-268139-tgpsu4qz	271	5	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	271	6	virus	virus	NN
cord-268139-tgpsu4qz	271	7	,	,	,
cord-268139-tgpsu4qz	271	8	an	an	DT
cord-268139-tgpsu4qz	271	9	additional	additional	JJ
cord-268139-tgpsu4qz	271	10	band	band	NN
cord-268139-tgpsu4qz	271	11	that	that	WDT
cord-268139-tgpsu4qz	271	12	migrated	migrate	VBD
cord-268139-tgpsu4qz	271	13	above	above	IN
cord-268139-tgpsu4qz	271	14	RNA4	RNA4	NNP
cord-268139-tgpsu4qz	271	15	was	be	VBD
cord-268139-tgpsu4qz	271	16	consistently	consistently	RB
cord-268139-tgpsu4qz	271	17	detected	detect	VBN
cord-268139-tgpsu4qz	271	18	.	.	.
cord-268139-tgpsu4qz	272	1	The	the	DT
cord-268139-tgpsu4qz	272	2	identity	identity	NN
cord-268139-tgpsu4qz	272	3	of	of	IN
cord-268139-tgpsu4qz	272	4	this	this	DT
cord-268139-tgpsu4qz	272	5	band	band	NN
cord-268139-tgpsu4qz	272	6	is	be	VBZ
cord-268139-tgpsu4qz	272	7	not	not	RB
cord-268139-tgpsu4qz	272	8	known	know	VBN
cord-268139-tgpsu4qz	272	9	,	,	,
cord-268139-tgpsu4qz	272	10	but	but	CC
cord-268139-tgpsu4qz	272	11	it	-PRON-	PRP
cord-268139-tgpsu4qz	272	12	might	may	MD
cord-268139-tgpsu4qz	272	13	represent	represent	VB
cord-268139-tgpsu4qz	272	14	a	a	DT
cord-268139-tgpsu4qz	272	15	more	more	RBR
cord-268139-tgpsu4qz	272	16	stable	stable	JJ
cord-268139-tgpsu4qz	272	17	replicative	replicative	JJ
cord-268139-tgpsu4qz	272	18	intermediate	intermediate	NN
cord-268139-tgpsu4qz	272	19	or	or	CC
cord-268139-tgpsu4qz	272	20	a	a	DT
cord-268139-tgpsu4qz	272	21	new	new	JJ
cord-268139-tgpsu4qz	272	22	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	272	23	RNA	RNA	NNP
cord-268139-tgpsu4qz	272	24	species	specie	NNS
cord-268139-tgpsu4qz	272	25	.	.	.
cord-268139-tgpsu4qz	273	1	Still	still	RB
cord-268139-tgpsu4qz	273	2	,	,	,
cord-268139-tgpsu4qz	273	3	the	the	DT
cord-268139-tgpsu4qz	273	4	approximate	approximate	JJ
cord-268139-tgpsu4qz	273	5	ratio	ratio	NN
cord-268139-tgpsu4qz	273	6	of	of	IN
cord-268139-tgpsu4qz	273	7	RNA1	RNA1	NNP
cord-268139-tgpsu4qz	273	8	(	(	-LRB-
cord-268139-tgpsu4qz	273	9	genome	genome	NN
cord-268139-tgpsu4qz	273	10	)	)	-RRB-
cord-268139-tgpsu4qz	273	11	to	to	IN
cord-268139-tgpsu4qz	273	12	RNA7	RNA7	NNP
cord-268139-tgpsu4qz	273	13	for	for	IN
cord-268139-tgpsu4qz	273	14	each	each	DT
cord-268139-tgpsu4qz	273	15	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	273	16	mutant	mutant	JJ
cord-268139-tgpsu4qz	273	17	was	be	VBD
cord-268139-tgpsu4qz	273	18	similar	similar	JJ
cord-268139-tgpsu4qz	273	19	to	to	IN
cord-268139-tgpsu4qz	273	20	that	that	DT
cord-268139-tgpsu4qz	273	21	of	of	IN
cord-268139-tgpsu4qz	273	22	icwt	icwt	NNP
cord-268139-tgpsu4qz	273	23	,	,	,
cord-268139-tgpsu4qz	273	24	suggesting	suggest	VBG
cord-268139-tgpsu4qz	273	25	that	that	IN
cord-268139-tgpsu4qz	273	26	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	273	27	mutants	mutant	NNS
cord-268139-tgpsu4qz	273	28	do	do	VBP
cord-268139-tgpsu4qz	273	29	not	not	RB
cord-268139-tgpsu4qz	273	30	have	have	VB
cord-268139-tgpsu4qz	273	31	specific	specific	JJ
cord-268139-tgpsu4qz	273	32	defects	defect	NNS
cord-268139-tgpsu4qz	273	33	in	in	IN
cord-268139-tgpsu4qz	273	34	either	either	DT
cord-268139-tgpsu4qz	273	35	genome	genome	NN
cord-268139-tgpsu4qz	273	36	replication	replication	NN
cord-268139-tgpsu4qz	273	37	or	or	CC
cord-268139-tgpsu4qz	273	38	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	273	39	RNA	RNA	NNP
cord-268139-tgpsu4qz	273	40	synthesis	synthesis	NN
cord-268139-tgpsu4qz	273	41	as	as	IN
cord-268139-tgpsu4qz	273	42	detected	detect	VBN
cord-268139-tgpsu4qz	273	43	by	by	IN
cord-268139-tgpsu4qz	273	44	this	this	DT
cord-268139-tgpsu4qz	273	45	assay	assay	NN
cord-268139-tgpsu4qz	273	46	.	.	.
cord-268139-tgpsu4qz	274	1	Coronavirus	Coronavirus	NNP
cord-268139-tgpsu4qz	274	2	gene	gene	NN
cord-268139-tgpsu4qz	274	3	1	1	CD
cord-268139-tgpsu4qz	274	4	nsps	nsp	NNS
cord-268139-tgpsu4qz	274	5	are	be	VBP
cord-268139-tgpsu4qz	274	6	predicted	predict	VBN
cord-268139-tgpsu4qz	274	7	to	to	TO
cord-268139-tgpsu4qz	274	8	function	function	VB
cord-268139-tgpsu4qz	274	9	in	in	IN
cord-268139-tgpsu4qz	274	10	the	the	DT
cord-268139-tgpsu4qz	274	11	synthesis	synthesis	NN
cord-268139-tgpsu4qz	274	12	of	of	IN
cord-268139-tgpsu4qz	274	13	viral	viral	JJ
cord-268139-tgpsu4qz	274	14	RNA	RNA	NNP
cord-268139-tgpsu4qz	274	15	at	at	IN
cord-268139-tgpsu4qz	274	16	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	274	17	replication	replication	NN
cord-268139-tgpsu4qz	274	18	complexes	complex	NNS
cord-268139-tgpsu4qz	274	19	;	;	:
cord-268139-tgpsu4qz	274	20	however	however	RB
cord-268139-tgpsu4qz	274	21	,	,	,
cord-268139-tgpsu4qz	274	22	many	many	JJ
cord-268139-tgpsu4qz	274	23	of	of	IN
cord-268139-tgpsu4qz	274	24	these	these	DT
cord-268139-tgpsu4qz	274	25	proteins	protein	NNS
cord-268139-tgpsu4qz	274	26	have	have	VBP
cord-268139-tgpsu4qz	274	27	no	no	DT
cord-268139-tgpsu4qz	274	28	known	know	VBN
cord-268139-tgpsu4qz	274	29	roles	role	NNS
cord-268139-tgpsu4qz	274	30	during	during	IN
cord-268139-tgpsu4qz	274	31	the	the	DT
cord-268139-tgpsu4qz	274	32	viral	viral	JJ
cord-268139-tgpsu4qz	274	33	life	life	NN
cord-268139-tgpsu4qz	274	34	cycle	cycle	NN
cord-268139-tgpsu4qz	274	35	.	.	.
cord-268139-tgpsu4qz	275	1	In	in	IN
cord-268139-tgpsu4qz	275	2	the	the	DT
cord-268139-tgpsu4qz	275	3	current	current	JJ
cord-268139-tgpsu4qz	275	4	study	study	NN
cord-268139-tgpsu4qz	275	5	,	,	,
cord-268139-tgpsu4qz	275	6	a	a	DT
cord-268139-tgpsu4qz	275	7	reverse	reverse	JJ
cord-268139-tgpsu4qz	275	8	genetic	genetic	JJ
cord-268139-tgpsu4qz	275	9	approach	approach	NN
cord-268139-tgpsu4qz	275	10	was	be	VBD
cord-268139-tgpsu4qz	275	11	used	use	VBN
cord-268139-tgpsu4qz	275	12	to	to	TO
cord-268139-tgpsu4qz	275	13	investigate	investigate	VB
cord-268139-tgpsu4qz	275	14	the	the	DT
cord-268139-tgpsu4qz	275	15	role	role	NN
cord-268139-tgpsu4qz	275	16	of	of	IN
cord-268139-tgpsu4qz	275	17	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	275	18	during	during	IN
cord-268139-tgpsu4qz	275	19	MHV	MHV	NNP
cord-268139-tgpsu4qz	275	20	replication	replication	NN
cord-268139-tgpsu4qz	275	21	in	in	IN
cord-268139-tgpsu4qz	275	22	cell	cell	NN
cord-268139-tgpsu4qz	275	23	culture	culture	NN
cord-268139-tgpsu4qz	275	24	and	and	CC
cord-268139-tgpsu4qz	275	25	to	to	TO
cord-268139-tgpsu4qz	275	26	identify	identify	VB
cord-268139-tgpsu4qz	275	27	residues	residue	NNS
cord-268139-tgpsu4qz	275	28	critical	critical	JJ
cord-268139-tgpsu4qz	275	29	for	for	IN
cord-268139-tgpsu4qz	275	30	its	-PRON-	PRP$
cord-268139-tgpsu4qz	275	31	function	function	NN
cord-268139-tgpsu4qz	275	32	.	.	.
cord-268139-tgpsu4qz	276	1	It	-PRON-	PRP
cord-268139-tgpsu4qz	276	2	was	be	VBD
cord-268139-tgpsu4qz	276	3	shown	show	VBN
cord-268139-tgpsu4qz	276	4	that	that	IN
cord-268139-tgpsu4qz	276	5	viruses	virus	NNS
cord-268139-tgpsu4qz	276	6	containing	contain	VBG
cord-268139-tgpsu4qz	276	7	deletions	deletion	NNS
cord-268139-tgpsu4qz	276	8	within	within	IN
cord-268139-tgpsu4qz	276	9	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	276	10	amino	amino	NN
cord-268139-tgpsu4qz	276	11	-	-	HYPH
cord-268139-tgpsu4qz	276	12	terminal	terminal	NN
cord-268139-tgpsu4qz	276	13	to	to	IN
cord-268139-tgpsu4qz	276	14	K	k	NN
cord-268139-tgpsu4qz	276	15	124	124	CD
cord-268139-tgpsu4qz	276	16	are	be	VBP
cord-268139-tgpsu4qz	276	17	not	not	RB
cord-268139-tgpsu4qz	276	18	capable	capable	JJ
cord-268139-tgpsu4qz	276	19	of	of	IN
cord-268139-tgpsu4qz	276	20	establishing	establish	VBG
cord-268139-tgpsu4qz	276	21	productive	productive	JJ
cord-268139-tgpsu4qz	276	22	infections	infection	NNS
cord-268139-tgpsu4qz	276	23	,	,	,
cord-268139-tgpsu4qz	276	24	suggesting	suggest	VBG
cord-268139-tgpsu4qz	276	25	that	that	IN
cord-268139-tgpsu4qz	276	26	residues	residue	NNS
cord-268139-tgpsu4qz	276	27	essential	essential	JJ
cord-268139-tgpsu4qz	276	28	for	for	IN
cord-268139-tgpsu4qz	276	29	MHV	MHV	NNP
cord-268139-tgpsu4qz	276	30	viability	viability	NN
cord-268139-tgpsu4qz	276	31	reside	reside	VBP
cord-268139-tgpsu4qz	276	32	within	within	IN
cord-268139-tgpsu4qz	276	33	the	the	DT
cord-268139-tgpsu4qz	276	34	amino	amino	NN
cord-268139-tgpsu4qz	276	35	-	-	HYPH
cord-268139-tgpsu4qz	276	36	terminal	terminal	NN
cord-268139-tgpsu4qz	276	37	half	half	NN
cord-268139-tgpsu4qz	276	38	of	of	IN
cord-268139-tgpsu4qz	276	39	the	the	DT
cord-268139-tgpsu4qz	276	40	protein	protein	NN
cord-268139-tgpsu4qz	276	41	.	.	.
cord-268139-tgpsu4qz	277	1	Several	several	JJ
cord-268139-tgpsu4qz	277	2	such	such	JJ
cord-268139-tgpsu4qz	277	3	residues	residue	NNS
cord-268139-tgpsu4qz	277	4	were	be	VBD
cord-268139-tgpsu4qz	277	5	identified	identify	VBN
cord-268139-tgpsu4qz	277	6	using	use	VBG
cord-268139-tgpsu4qz	277	7	point	point	NN
cord-268139-tgpsu4qz	277	8	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	277	9	,	,	,
cord-268139-tgpsu4qz	277	10	and	and	CC
cord-268139-tgpsu4qz	277	11	the	the	DT
cord-268139-tgpsu4qz	277	12	importance	importance	NN
cord-268139-tgpsu4qz	277	13	of	of	IN
cord-268139-tgpsu4qz	277	14	the	the	DT
cord-268139-tgpsu4qz	277	15	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	277	16	for	for	IN
cord-268139-tgpsu4qz	277	17	viral	viral	JJ
cord-268139-tgpsu4qz	277	18	replication	replication	NN
cord-268139-tgpsu4qz	277	19	and	and	CC
cord-268139-tgpsu4qz	277	20	RNA	RNA	NNP
cord-268139-tgpsu4qz	277	21	synthesis	synthesis	NN
cord-268139-tgpsu4qz	277	22	was	be	VBD
cord-268139-tgpsu4qz	277	23	characterized	characterize	VBN
cord-268139-tgpsu4qz	277	24	.	.	.
cord-268139-tgpsu4qz	278	1	Moreover	moreover	RB
cord-268139-tgpsu4qz	278	2	,	,	,
cord-268139-tgpsu4qz	278	3	the	the	DT
cord-268139-tgpsu4qz	278	4	results	result	NNS
cord-268139-tgpsu4qz	278	5	show	show	VBP
cord-268139-tgpsu4qz	278	6	that	that	IN
cord-268139-tgpsu4qz	278	7	the	the	DT
cord-268139-tgpsu4qz	278	8	carboxyterminal	carboxyterminal	JJ
cord-268139-tgpsu4qz	278	9	half	half	NN
cord-268139-tgpsu4qz	278	10	of	of	IN
cord-268139-tgpsu4qz	278	11	nsp1	nsp1	CD
cord-268139-tgpsu4qz	278	12	is	be	VBZ
cord-268139-tgpsu4qz	278	13	not	not	RB
cord-268139-tgpsu4qz	278	14	required	require	VBN
cord-268139-tgpsu4qz	278	15	for	for	IN
cord-268139-tgpsu4qz	278	16	MHV	MHV	NNP
cord-268139-tgpsu4qz	278	17	to	to	TO
cord-268139-tgpsu4qz	278	18	complete	complete	VB
cord-268139-tgpsu4qz	278	19	its	-PRON-	PRP$
cord-268139-tgpsu4qz	278	20	life	life	NN
cord-268139-tgpsu4qz	278	21	cycle	cycle	NN
cord-268139-tgpsu4qz	278	22	but	but	CC
cord-268139-tgpsu4qz	278	23	is	be	VBZ
cord-268139-tgpsu4qz	278	24	necessary	necessary	JJ
cord-268139-tgpsu4qz	278	25	for	for	IN
cord-268139-tgpsu4qz	278	26	efficient	efficient	JJ
cord-268139-tgpsu4qz	278	27	cleavage	cleavage	NN
cord-268139-tgpsu4qz	278	28	of	of	IN
cord-268139-tgpsu4qz	278	29	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	278	30	from	from	IN
cord-268139-tgpsu4qz	278	31	the	the	DT
cord-268139-tgpsu4qz	278	32	gene	gene	NN
cord-268139-tgpsu4qz	278	33	1	1	CD
cord-268139-tgpsu4qz	278	34	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	278	35	and	and	CC
cord-268139-tgpsu4qz	278	36	for	for	IN
cord-268139-tgpsu4qz	278	37	optimal	optimal	JJ
cord-268139-tgpsu4qz	278	38	viral	viral	JJ
cord-268139-tgpsu4qz	278	39	replication	replication	NN
cord-268139-tgpsu4qz	278	40	.	.	.
cord-268139-tgpsu4qz	279	1	Together	together	RB
cord-268139-tgpsu4qz	279	2	,	,	,
cord-268139-tgpsu4qz	279	3	these	these	DT
cord-268139-tgpsu4qz	279	4	data	datum	NNS
cord-268139-tgpsu4qz	279	5	are	be	VBP
cord-268139-tgpsu4qz	279	6	consistent	consistent	JJ
cord-268139-tgpsu4qz	279	7	with	with	IN
cord-268139-tgpsu4qz	279	8	the	the	DT
cord-268139-tgpsu4qz	279	9	hypothesis	hypothesis	NN
cord-268139-tgpsu4qz	279	10	that	that	WDT
cord-268139-tgpsu4qz	279	11	MHV	MHV	NNP
cord-268139-tgpsu4qz	279	12	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	279	13	contains	contain	VBZ
cord-268139-tgpsu4qz	279	14	at	at	IN
cord-268139-tgpsu4qz	279	15	least	least	RBS
cord-268139-tgpsu4qz	279	16	two	two	CD
cord-268139-tgpsu4qz	279	17	domains	domain	NNS
cord-268139-tgpsu4qz	279	18	important	important	JJ
cord-268139-tgpsu4qz	279	19	for	for	IN
cord-268139-tgpsu4qz	279	20	virus	virus	NN
cord-268139-tgpsu4qz	279	21	replication	replication	NN
cord-268139-tgpsu4qz	279	22	:	:	:
cord-268139-tgpsu4qz	279	23	(	(	-LRB-
cord-268139-tgpsu4qz	279	24	1	1	LS
cord-268139-tgpsu4qz	279	25	)	)	-RRB-
cord-268139-tgpsu4qz	279	26	an	an	DT
cord-268139-tgpsu4qz	279	27	essential	essential	JJ
cord-268139-tgpsu4qz	279	28	aminoterminal	aminoterminal	JJ
cord-268139-tgpsu4qz	279	29	domain	domain	NN
cord-268139-tgpsu4qz	279	30	involved	involve	VBN
cord-268139-tgpsu4qz	279	31	in	in	IN
cord-268139-tgpsu4qz	279	32	viral	viral	JJ
cord-268139-tgpsu4qz	279	33	RNA	RNA	NNP
cord-268139-tgpsu4qz	279	34	synthesis	synthesis	NN
cord-268139-tgpsu4qz	279	35	and	and	CC
cord-268139-tgpsu4qz	279	36	(	(	-LRB-
cord-268139-tgpsu4qz	279	37	2	2	LS
cord-268139-tgpsu4qz	279	38	)	)	-RRB-
cord-268139-tgpsu4qz	279	39	a	a	DT
cord-268139-tgpsu4qz	279	40	non	non	JJ
cord-268139-tgpsu4qz	279	41	-	-	JJ
cord-268139-tgpsu4qz	279	42	essential	essential	JJ
cord-268139-tgpsu4qz	279	43	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	279	44	-	-	HYPH
cord-268139-tgpsu4qz	279	45	terminal	terminal	NN
cord-268139-tgpsu4qz	279	46	domain	domain	NN
cord-268139-tgpsu4qz	279	47	that	that	WDT
cord-268139-tgpsu4qz	279	48	influences	influence	VBZ
cord-268139-tgpsu4qz	279	49	CS1	CS1	NNP
cord-268139-tgpsu4qz	279	50	cleavage	cleavage	NN
cord-268139-tgpsu4qz	279	51	efficiency	efficiency	NN
cord-268139-tgpsu4qz	279	52	.	.	.
cord-268139-tgpsu4qz	280	1	The	the	DT
cord-268139-tgpsu4qz	280	2	analyses	analysis	NNS
cord-268139-tgpsu4qz	280	3	of	of	IN
cord-268139-tgpsu4qz	280	4	MHV	MHV	NNP
cord-268139-tgpsu4qz	280	5	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	280	6	deletion	deletion	NN
cord-268139-tgpsu4qz	280	7	and	and	CC
cord-268139-tgpsu4qz	280	8	point	point	NN
cord-268139-tgpsu4qz	280	9	mutants	mutant	NNS
cord-268139-tgpsu4qz	280	10	emphasize	emphasize	VBP
cord-268139-tgpsu4qz	280	11	the	the	DT
cord-268139-tgpsu4qz	280	12	significance	significance	NN
cord-268139-tgpsu4qz	280	13	of	of	IN
cord-268139-tgpsu4qz	280	14	the	the	DT
cord-268139-tgpsu4qz	280	15	amino	amino	NN
cord-268139-tgpsu4qz	280	16	-	-	HYPH
cord-268139-tgpsu4qz	280	17	terminus	terminus	NN
cord-268139-tgpsu4qz	280	18	for	for	IN
cord-268139-tgpsu4qz	280	19	virus	virus	NN
cord-268139-tgpsu4qz	280	20	viability	viability	NN
cord-268139-tgpsu4qz	280	21	,	,	,
cord-268139-tgpsu4qz	280	22	replication	replication	NN
cord-268139-tgpsu4qz	280	23	,	,	,
cord-268139-tgpsu4qz	280	24	and	and	CC
cord-268139-tgpsu4qz	280	25	RNA	RNA	NNP
cord-268139-tgpsu4qz	280	26	synthesis	synthesis	NN
cord-268139-tgpsu4qz	280	27	.	.	.
cord-268139-tgpsu4qz	281	1	Deletion	deletion	NN
cord-268139-tgpsu4qz	281	2	of	of	IN
cord-268139-tgpsu4qz	281	3	different	different	JJ
cord-268139-tgpsu4qz	281	4	portions	portion	NNS
cord-268139-tgpsu4qz	281	5	of	of	IN
cord-268139-tgpsu4qz	281	6	the	the	DT
cord-268139-tgpsu4qz	281	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	281	8	amino	amino	NN
cord-268139-tgpsu4qz	281	9	-	-	HYPH
cord-268139-tgpsu4qz	281	10	terminal	terminal	NN
cord-268139-tgpsu4qz	281	11	half	half	NN
cord-268139-tgpsu4qz	281	12	,	,	,
cord-268139-tgpsu4qz	281	13	as	as	IN
cord-268139-tgpsu4qz	281	14	was	be	VBD
cord-268139-tgpsu4qz	281	15	done	do	VBN
cord-268139-tgpsu4qz	281	16	with	with	IN
cord-268139-tgpsu4qz	281	17	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	281	18	and	and	CC
cord-268139-tgpsu4qz	281	19	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	281	20	,	,	,
cord-268139-tgpsu4qz	281	21	was	be	VBD
cord-268139-tgpsu4qz	281	22	not	not	RB
cord-268139-tgpsu4qz	281	23	tolerated	tolerate	VBN
cord-268139-tgpsu4qz	281	24	for	for	IN
cord-268139-tgpsu4qz	281	25	virus	virus	NN
cord-268139-tgpsu4qz	281	26	viability	viability	NN
cord-268139-tgpsu4qz	281	27	.	.	.
cord-268139-tgpsu4qz	282	1	This	this	DT
cord-268139-tgpsu4qz	282	2	result	result	NN
cord-268139-tgpsu4qz	282	3	is	be	VBZ
cord-268139-tgpsu4qz	282	4	consistent	consistent	JJ
cord-268139-tgpsu4qz	282	5	with	with	IN
cord-268139-tgpsu4qz	282	6	the	the	DT
cord-268139-tgpsu4qz	282	7	idea	idea	NN
cord-268139-tgpsu4qz	282	8	that	that	IN
cord-268139-tgpsu4qz	282	9	critical	critical	JJ
cord-268139-tgpsu4qz	282	10	replication	replication	NN
cord-268139-tgpsu4qz	282	11	determinants	determinant	NNS
cord-268139-tgpsu4qz	282	12	reside	reside	VBP
cord-268139-tgpsu4qz	282	13	within	within	IN
cord-268139-tgpsu4qz	282	14	the	the	DT
cord-268139-tgpsu4qz	282	15	region	region	NN
cord-268139-tgpsu4qz	282	16	spanning	span	VBG
cord-268139-tgpsu4qz	282	17	residues	residue	NNS
cord-268139-tgpsu4qz	282	18	M	M	NNP
cord-268139-tgpsu4qz	282	19	1	1	CD
cord-268139-tgpsu4qz	282	20	through	through	IN
cord-268139-tgpsu4qz	282	21	P	p	NN
cord-268139-tgpsu4qz	282	22	123	123	CD
cord-268139-tgpsu4qz	282	23	.	.	.
cord-268139-tgpsu4qz	283	1	Charge	charge	NN
cord-268139-tgpsu4qz	283	2	-	-	HYPH
cord-268139-tgpsu4qz	283	3	to	to	IN
cord-268139-tgpsu4qz	283	4	-	-	HYPH
cord-268139-tgpsu4qz	283	5	alanine	alanine	NN
cord-268139-tgpsu4qz	283	6	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	283	7	identified	identify	VBD
cord-268139-tgpsu4qz	283	8	four	four	CD
cord-268139-tgpsu4qz	283	9	candidate	candidate	NN
cord-268139-tgpsu4qz	283	10	residues	residue	NNS
cord-268139-tgpsu4qz	283	11	(	(	-LRB-
cord-268139-tgpsu4qz	283	12	R	R	NNP
cord-268139-tgpsu4qz	283	13	64	64	CD
cord-268139-tgpsu4qz	283	14	and	and	CC
cord-268139-tgpsu4qz	283	15	E	E	NNP
cord-268139-tgpsu4qz	283	16	69	69	CD
cord-268139-tgpsu4qz	283	17	with	with	IN
cord-268139-tgpsu4qz	283	18	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	283	19	;	;	.
cord-268139-tgpsu4qz	284	1	R	R	NNP
cord-268139-tgpsu4qz	284	2	78	78	CD
cord-268139-tgpsu4qz	284	3	and	and	CC
cord-268139-tgpsu4qz	284	4	D	D	NNP
cord-268139-tgpsu4qz	284	5	79	79	CD
cord-268139-tgpsu4qz	284	6	with	with	IN
cord-268139-tgpsu4qz	284	7	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	284	8	)	)	-RRB-
cord-268139-tgpsu4qz	284	9	,	,	,
cord-268139-tgpsu4qz	284	10	all	all	DT
cord-268139-tgpsu4qz	284	11	or	or	CC
cord-268139-tgpsu4qz	284	12	any	any	DT
cord-268139-tgpsu4qz	284	13	of	of	IN
cord-268139-tgpsu4qz	284	14	which	which	WDT
cord-268139-tgpsu4qz	284	15	may	may	MD
cord-268139-tgpsu4qz	284	16	be	be	VB
cord-268139-tgpsu4qz	284	17	vital	vital	JJ
cord-268139-tgpsu4qz	284	18	for	for	IN
cord-268139-tgpsu4qz	284	19	some	some	DT
cord-268139-tgpsu4qz	284	20	stage	stage	NN
cord-268139-tgpsu4qz	284	21	of	of	IN
cord-268139-tgpsu4qz	284	22	the	the	DT
cord-268139-tgpsu4qz	284	23	MHV	MHV	NNP
cord-268139-tgpsu4qz	284	24	life	life	NN
cord-268139-tgpsu4qz	284	25	cycle	cycle	NN
cord-268139-tgpsu4qz	284	26	.	.	.
cord-268139-tgpsu4qz	285	1	In	in	IN
cord-268139-tgpsu4qz	285	2	cells	cell	NNS
cord-268139-tgpsu4qz	285	3	transfected	transfecte	VBN
cord-268139-tgpsu4qz	285	4	with	with	IN
cord-268139-tgpsu4qz	285	5	RNA	RNA	NNP
cord-268139-tgpsu4qz	285	6	genomes	genome	NNS
cord-268139-tgpsu4qz	285	7	for	for	IN
cord-268139-tgpsu4qz	285	8	these	these	DT
cord-268139-tgpsu4qz	285	9	nonproductive	nonproductive	JJ
cord-268139-tgpsu4qz	285	10	mutants	mutant	NNS
cord-268139-tgpsu4qz	285	11	(	(	-LRB-
cord-268139-tgpsu4qz	285	12	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	285	13	,	,	,
cord-268139-tgpsu4qz	285	14	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	285	15	,	,	,
cord-268139-tgpsu4qz	285	16	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	285	17	,	,	,
cord-268139-tgpsu4qz	285	18	or	or	CC
cord-268139-tgpsu4qz	285	19	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	285	20	)	)	-RRB-
cord-268139-tgpsu4qz	285	21	,	,	,
cord-268139-tgpsu4qz	285	22	foci	foci	NN
cord-268139-tgpsu4qz	285	23	containing	contain	VBG
cord-268139-tgpsu4qz	285	24	5	5	CD
cord-268139-tgpsu4qz	285	25	-10	-10	NN
cord-268139-tgpsu4qz	285	26	cell	cell	NN
cord-268139-tgpsu4qz	285	27	nuclei	nucleus	NNS
cord-268139-tgpsu4qz	285	28	were	be	VBD
cord-268139-tgpsu4qz	285	29	seen	see	VBN
cord-268139-tgpsu4qz	285	30	.	.	.
cord-268139-tgpsu4qz	286	1	How	how	WRB
cord-268139-tgpsu4qz	286	2	these	these	DT
cord-268139-tgpsu4qz	286	3	foci	foci	NN
cord-268139-tgpsu4qz	286	4	form	form	NN
cord-268139-tgpsu4qz	286	5	in	in	IN
cord-268139-tgpsu4qz	286	6	electroporated	electroporated	JJ
cord-268139-tgpsu4qz	286	7	cell	cell	NN
cord-268139-tgpsu4qz	286	8	monolayers	monolayer	NNS
cord-268139-tgpsu4qz	286	9	is	be	VBZ
cord-268139-tgpsu4qz	286	10	unknown	unknown	JJ
cord-268139-tgpsu4qz	286	11	,	,	,
cord-268139-tgpsu4qz	286	12	but	but	CC
cord-268139-tgpsu4qz	286	13	the	the	DT
cord-268139-tgpsu4qz	286	14	presence	presence	NN
cord-268139-tgpsu4qz	286	15	of	of	IN
cord-268139-tgpsu4qz	286	16	this	this	DT
cord-268139-tgpsu4qz	286	17	CPE	CPE	NNP
cord-268139-tgpsu4qz	286	18	suggests	suggest	VBZ
cord-268139-tgpsu4qz	286	19	that	that	IN
cord-268139-tgpsu4qz	286	20	transient	transient	JJ
cord-268139-tgpsu4qz	286	21	viral	viral	JJ
cord-268139-tgpsu4qz	286	22	gene	gene	NN
cord-268139-tgpsu4qz	286	23	expression	expression	NN
cord-268139-tgpsu4qz	286	24	may	may	MD
cord-268139-tgpsu4qz	286	25	have	have	VB
cord-268139-tgpsu4qz	286	26	occurred	occur	VBN
cord-268139-tgpsu4qz	286	27	.	.	.
cord-268139-tgpsu4qz	287	1	However	however	RB
cord-268139-tgpsu4qz	287	2	,	,	,
cord-268139-tgpsu4qz	287	3	no	no	DT
cord-268139-tgpsu4qz	287	4	viral	viral	JJ
cord-268139-tgpsu4qz	287	5	protein	protein	NN
cord-268139-tgpsu4qz	287	6	was	be	VBD
cord-268139-tgpsu4qz	287	7	detected	detect	VBN
cord-268139-tgpsu4qz	287	8	in	in	IN
cord-268139-tgpsu4qz	287	9	transfected	transfecte	VBN
cord-268139-tgpsu4qz	287	10	cell	cell	NN
cord-268139-tgpsu4qz	287	11	monolayers	monolayer	NNS
cord-268139-tgpsu4qz	287	12	by	by	IN
cord-268139-tgpsu4qz	287	13	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	287	14	or	or	CC
cord-268139-tgpsu4qz	287	15	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	287	16	assays	assay	NNS
cord-268139-tgpsu4qz	287	17	,	,	,
cord-268139-tgpsu4qz	287	18	and	and	CC
cord-268139-tgpsu4qz	287	19	RT	RT	NNP
cord-268139-tgpsu4qz	287	20	-	-	HYPH
cord-268139-tgpsu4qz	287	21	PCR	PCR	NNP
cord-268139-tgpsu4qz	287	22	did	do	VBD
cord-268139-tgpsu4qz	287	23	not	not	RB
cord-268139-tgpsu4qz	287	24	amplify	amplify	VB
cord-268139-tgpsu4qz	287	25	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	287	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	287	27	,	,	,
cord-268139-tgpsu4qz	287	28	indicating	indicate	VBG
cord-268139-tgpsu4qz	287	29	that	that	IN
cord-268139-tgpsu4qz	287	30	,	,	,
cord-268139-tgpsu4qz	287	31	if	if	IN
cord-268139-tgpsu4qz	287	32	gene	gene	NN
cord-268139-tgpsu4qz	287	33	expression	expression	NN
cord-268139-tgpsu4qz	287	34	occurred	occur	VBD
cord-268139-tgpsu4qz	287	35	,	,	,
cord-268139-tgpsu4qz	287	36	it	-PRON-	PRP
cord-268139-tgpsu4qz	287	37	was	be	VBD
cord-268139-tgpsu4qz	287	38	below	below	IN
cord-268139-tgpsu4qz	287	39	the	the	DT
cord-268139-tgpsu4qz	287	40	limits	limit	NNS
cord-268139-tgpsu4qz	287	41	of	of	IN
cord-268139-tgpsu4qz	287	42	detection	detection	NN
cord-268139-tgpsu4qz	287	43	using	use	VBG
cord-268139-tgpsu4qz	287	44	these	these	DT
cord-268139-tgpsu4qz	287	45	assays	assay	NNS
cord-268139-tgpsu4qz	287	46	.	.	.
cord-268139-tgpsu4qz	288	1	Consequently	consequently	RB
cord-268139-tgpsu4qz	288	2	,	,	,
cord-268139-tgpsu4qz	288	3	the	the	DT
cord-268139-tgpsu4qz	288	4	exact	exact	JJ
cord-268139-tgpsu4qz	288	5	stage	stage	NN
cord-268139-tgpsu4qz	288	6	of	of	IN
cord-268139-tgpsu4qz	288	7	the	the	DT
cord-268139-tgpsu4qz	288	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	288	9	life	life	NN
cord-268139-tgpsu4qz	288	10	cycle	cycle	NN
cord-268139-tgpsu4qz	288	11	at	at	IN
cord-268139-tgpsu4qz	288	12	which	which	WDT
cord-268139-tgpsu4qz	288	13	these	these	DT
cord-268139-tgpsu4qz	288	14	mutant	mutant	JJ
cord-268139-tgpsu4qz	288	15	viruses	virus	NNS
cord-268139-tgpsu4qz	288	16	are	be	VBP
cord-268139-tgpsu4qz	288	17	blocked	block	VBN
cord-268139-tgpsu4qz	288	18	could	could	MD
cord-268139-tgpsu4qz	288	19	not	not	RB
cord-268139-tgpsu4qz	288	20	be	be	VB
cord-268139-tgpsu4qz	288	21	determined	determine	VBN
cord-268139-tgpsu4qz	288	22	.	.	.
cord-268139-tgpsu4qz	289	1	Still	still	RB
cord-268139-tgpsu4qz	289	2	,	,	,
cord-268139-tgpsu4qz	289	3	the	the	DT
cord-268139-tgpsu4qz	289	4	result	result	NN
cord-268139-tgpsu4qz	289	5	that	that	IN
cord-268139-tgpsu4qz	289	6	nsp1DFL	nsp1DFL	NNP
cord-268139-tgpsu4qz	289	7	,	,	,
cord-268139-tgpsu4qz	289	8	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	289	9	,	,	,
cord-268139-tgpsu4qz	289	10	VUSB5	vusb5	NN
cord-268139-tgpsu4qz	289	11	,	,	,
cord-268139-tgpsu4qz	289	12	or	or	CC
cord-268139-tgpsu4qz	289	13	VUSB6	VUSB6	NNP
cord-268139-tgpsu4qz	289	14	did	do	VBD
cord-268139-tgpsu4qz	289	15	not	not	RB
cord-268139-tgpsu4qz	289	16	establish	establish	VB
cord-268139-tgpsu4qz	289	17	productive	productive	JJ
cord-268139-tgpsu4qz	289	18	infections	infection	NNS
cord-268139-tgpsu4qz	289	19	underscores	underscore	VBZ
cord-268139-tgpsu4qz	289	20	the	the	DT
cord-268139-tgpsu4qz	289	21	importance	importance	NN
cord-268139-tgpsu4qz	289	22	of	of	IN
cord-268139-tgpsu4qz	289	23	the	the	DT
cord-268139-tgpsu4qz	289	24	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	289	25	amino	amino	NN
cord-268139-tgpsu4qz	289	26	-	-	HYPH
cord-268139-tgpsu4qz	289	27	terminus	terminus	NN
cord-268139-tgpsu4qz	289	28	and	and	CC
cord-268139-tgpsu4qz	289	29	suggests	suggest	VBZ
cord-268139-tgpsu4qz	289	30	that	that	IN
cord-268139-tgpsu4qz	289	31	this	this	DT
cord-268139-tgpsu4qz	289	32	protein	protein	NN
cord-268139-tgpsu4qz	289	33	has	have	VBZ
cord-268139-tgpsu4qz	289	34	a	a	DT
cord-268139-tgpsu4qz	289	35	critical	critical	JJ
cord-268139-tgpsu4qz	289	36	function	function	NN
cord-268139-tgpsu4qz	289	37	during	during	IN
cord-268139-tgpsu4qz	289	38	viral	viral	JJ
cord-268139-tgpsu4qz	289	39	replication	replication	NN
cord-268139-tgpsu4qz	289	40	.	.	.
cord-268139-tgpsu4qz	290	1	In	in	IN
cord-268139-tgpsu4qz	290	2	support	support	NN
cord-268139-tgpsu4qz	290	3	of	of	IN
cord-268139-tgpsu4qz	290	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	290	5	having	have	VBG
cord-268139-tgpsu4qz	290	6	a	a	DT
cord-268139-tgpsu4qz	290	7	central	central	JJ
cord-268139-tgpsu4qz	290	8	role	role	NN
cord-268139-tgpsu4qz	290	9	in	in	IN
cord-268139-tgpsu4qz	290	10	the	the	DT
cord-268139-tgpsu4qz	290	11	MHV	MHV	NNP
cord-268139-tgpsu4qz	290	12	life	life	NN
cord-268139-tgpsu4qz	290	13	cycle	cycle	NN
cord-268139-tgpsu4qz	290	14	,	,	,
cord-268139-tgpsu4qz	290	15	it	-PRON-	PRP
cord-268139-tgpsu4qz	290	16	was	be	VBD
cord-268139-tgpsu4qz	290	17	demonstrated	demonstrate	VBN
cord-268139-tgpsu4qz	290	18	that	that	IN
cord-268139-tgpsu4qz	290	19	all	all	DT
cord-268139-tgpsu4qz	290	20	of	of	IN
cord-268139-tgpsu4qz	290	21	the	the	DT
cord-268139-tgpsu4qz	290	22	viable	viable	JJ
cord-268139-tgpsu4qz	290	23	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	290	24	point	point	NN
cord-268139-tgpsu4qz	290	25	mutants	mutant	NNS
cord-268139-tgpsu4qz	290	26	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	290	27	reduced	reduce	VBN
cord-268139-tgpsu4qz	290	28	viral	viral	JJ
cord-268139-tgpsu4qz	290	29	yields	yield	NNS
cord-268139-tgpsu4qz	290	30	during	during	IN
cord-268139-tgpsu4qz	290	31	singlecycle	singlecycle	NNP
cord-268139-tgpsu4qz	290	32	replication	replication	NN
cord-268139-tgpsu4qz	290	33	assays	assay	NNS
cord-268139-tgpsu4qz	290	34	.	.	.
cord-268139-tgpsu4qz	291	1	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	291	2	and	and	CC
cord-268139-tgpsu4qz	291	3	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	291	4	were	be	VBD
cord-268139-tgpsu4qz	291	5	the	the	DT
cord-268139-tgpsu4qz	291	6	most	most	RBS
cord-268139-tgpsu4qz	291	7	defective	defective	JJ
cord-268139-tgpsu4qz	291	8	and	and	CC
cord-268139-tgpsu4qz	291	9	resulted	result	VBD
cord-268139-tgpsu4qz	291	10	in	in	IN
cord-268139-tgpsu4qz	291	11	viral	viral	JJ
cord-268139-tgpsu4qz	291	12	titers	titer	NNS
cord-268139-tgpsu4qz	291	13	approximately	approximately	RB
cord-268139-tgpsu4qz	291	14	0.2-to	0.2-to	CD
cord-268139-tgpsu4qz	291	15	1.3-log	1.3-log	CD
cord-268139-tgpsu4qz	291	16	reduced	reduce	VBN
cord-268139-tgpsu4qz	291	17	compared	compare	VBN
cord-268139-tgpsu4qz	291	18	with	with	IN
cord-268139-tgpsu4qz	291	19	icwt	icwt	NNP
cord-268139-tgpsu4qz	291	20	.	.	.
cord-268139-tgpsu4qz	292	1	These	these	DT
cord-268139-tgpsu4qz	292	2	two	two	CD
cord-268139-tgpsu4qz	292	3	viruses	virus	NNS
cord-268139-tgpsu4qz	292	4	also	also	RB
cord-268139-tgpsu4qz	292	5	exhibited	exhibit	VBD
cord-268139-tgpsu4qz	292	6	small	small	JJ
cord-268139-tgpsu4qz	292	7	plaque	plaque	NN
cord-268139-tgpsu4qz	292	8	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	292	9	,	,	,
cord-268139-tgpsu4qz	292	10	suggesting	suggest	VBG
cord-268139-tgpsu4qz	292	11	defects	defect	NNS
cord-268139-tgpsu4qz	292	12	in	in	IN
cord-268139-tgpsu4qz	292	13	cell	cell	NN
cord-268139-tgpsu4qz	292	14	-	-	HYPH
cord-268139-tgpsu4qz	292	15	to	to	IN
cord-268139-tgpsu4qz	292	16	-	-	HYPH
cord-268139-tgpsu4qz	292	17	cell	cell	NN
cord-268139-tgpsu4qz	292	18	spread	spread	NN
cord-268139-tgpsu4qz	292	19	.	.	.
cord-268139-tgpsu4qz	293	1	The	the	DT
cord-268139-tgpsu4qz	293	2	reduced	reduce	VBN
cord-268139-tgpsu4qz	293	3	replication	replication	NN
cord-268139-tgpsu4qz	293	4	and	and	CC
cord-268139-tgpsu4qz	293	5	small	small	JJ
cord-268139-tgpsu4qz	293	6	plaques	plaque	NNS
cord-268139-tgpsu4qz	293	7	seen	see	VBN
cord-268139-tgpsu4qz	293	8	with	with	IN
cord-268139-tgpsu4qz	293	9	VUSB1	vusb1	NN
cord-268139-tgpsu4qz	294	1	and	and	CC
cord-268139-tgpsu4qz	294	2	VUSB4	VUSB4	NNS
cord-268139-tgpsu4qz	294	3	are	be	VBP
cord-268139-tgpsu4qz	294	4	most	most	RBS
cord-268139-tgpsu4qz	294	5	likely	likely	RB
cord-268139-tgpsu4qz	294	6	a	a	DT
cord-268139-tgpsu4qz	294	7	reflection	reflection	NN
cord-268139-tgpsu4qz	294	8	of	of	IN
cord-268139-tgpsu4qz	294	9	reduced	reduce	VBN
cord-268139-tgpsu4qz	294	10	viral	viral	JJ
cord-268139-tgpsu4qz	294	11	gene	gene	NN
cord-268139-tgpsu4qz	294	12	expression	expression	NN
cord-268139-tgpsu4qz	294	13	as	as	IN
cord-268139-tgpsu4qz	294	14	both	both	DT
cord-268139-tgpsu4qz	294	15	of	of	IN
cord-268139-tgpsu4qz	294	16	these	these	DT
cord-268139-tgpsu4qz	294	17	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	294	18	point	point	NN
cord-268139-tgpsu4qz	294	19	mutants	mutant	NNS
cord-268139-tgpsu4qz	294	20	showed	show	VBD
cord-268139-tgpsu4qz	294	21	significantly	significantly	RB
cord-268139-tgpsu4qz	294	22	lower	low	JJR
cord-268139-tgpsu4qz	294	23	levels	level	NNS
cord-268139-tgpsu4qz	294	24	of	of	IN
cord-268139-tgpsu4qz	294	25	viral	viral	JJ
cord-268139-tgpsu4qz	294	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	294	27	.	.	.
cord-268139-tgpsu4qz	295	1	Interestingly	interestingly	RB
cord-268139-tgpsu4qz	295	2	,	,	,
cord-268139-tgpsu4qz	295	3	compared	compare	VBN
cord-268139-tgpsu4qz	295	4	with	with	IN
cord-268139-tgpsu4qz	295	5	icwt	icwt	NNP
cord-268139-tgpsu4qz	295	6	,	,	,
cord-268139-tgpsu4qz	295	7	VUSB3	VUSB3	NNP
cord-268139-tgpsu4qz	295	8	had	have	VBD
cord-268139-tgpsu4qz	295	9	increased	increase	VBN
cord-268139-tgpsu4qz	295	10	levels	level	NNS
cord-268139-tgpsu4qz	295	11	of	of	IN
cord-268139-tgpsu4qz	295	12	viral	viral	JJ
cord-268139-tgpsu4qz	295	13	RNA	rna	NN
cord-268139-tgpsu4qz	295	14	associated	associate	VBN
cord-268139-tgpsu4qz	295	15	with	with	IN
cord-268139-tgpsu4qz	295	16	a	a	DT
cord-268139-tgpsu4qz	295	17	slight	slight	JJ
cord-268139-tgpsu4qz	295	18	reduction	reduction	NN
cord-268139-tgpsu4qz	295	19	in	in	IN
cord-268139-tgpsu4qz	295	20	viral	viral	JJ
cord-268139-tgpsu4qz	295	21	replication	replication	NN
cord-268139-tgpsu4qz	295	22	,	,	,
cord-268139-tgpsu4qz	295	23	suggesting	suggest	VBG
cord-268139-tgpsu4qz	295	24	that	that	IN
cord-268139-tgpsu4qz	295	25	any	any	DT
cord-268139-tgpsu4qz	295	26	deviation	deviation	NN
cord-268139-tgpsu4qz	295	27	from	from	IN
cord-268139-tgpsu4qz	295	28	wild	wild	JJ
cord-268139-tgpsu4qz	295	29	-	-	HYPH
cord-268139-tgpsu4qz	295	30	type	type	NN
cord-268139-tgpsu4qz	295	31	viral	viral	JJ
cord-268139-tgpsu4qz	295	32	RNA	RNA	NNP
cord-268139-tgpsu4qz	295	33	levels	level	NNS
cord-268139-tgpsu4qz	295	34	may	may	MD
cord-268139-tgpsu4qz	295	35	negatively	negatively	RB
cord-268139-tgpsu4qz	295	36	impact	impact	VB
cord-268139-tgpsu4qz	295	37	viral	viral	JJ
cord-268139-tgpsu4qz	295	38	replication	replication	NN
cord-268139-tgpsu4qz	295	39	.	.	.
cord-268139-tgpsu4qz	296	1	There	there	EX
cord-268139-tgpsu4qz	296	2	were	be	VBD
cord-268139-tgpsu4qz	296	3	no	no	DT
cord-268139-tgpsu4qz	296	4	detectable	detectable	JJ
cord-268139-tgpsu4qz	296	5	differences	difference	NNS
cord-268139-tgpsu4qz	296	6	in	in	IN
cord-268139-tgpsu4qz	296	7	the	the	DT
cord-268139-tgpsu4qz	296	8	ratios	ratio	NNS
cord-268139-tgpsu4qz	296	9	of	of	IN
cord-268139-tgpsu4qz	296	10	genome	genome	NN
cord-268139-tgpsu4qz	296	11	RNA	RNA	NNP
cord-268139-tgpsu4qz	296	12	versus	versus	IN
cord-268139-tgpsu4qz	296	13	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	296	14	RNAs	RNAs	NNPS
cord-268139-tgpsu4qz	296	15	for	for	IN
cord-268139-tgpsu4qz	296	16	any	any	DT
cord-268139-tgpsu4qz	296	17	of	of	IN
cord-268139-tgpsu4qz	296	18	the	the	DT
cord-268139-tgpsu4qz	296	19	viable	viable	JJ
cord-268139-tgpsu4qz	296	20	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	296	21	mutants	mutant	NNS
cord-268139-tgpsu4qz	296	22	.	.	.
cord-268139-tgpsu4qz	297	1	These	these	DT
cord-268139-tgpsu4qz	297	2	results	result	NNS
cord-268139-tgpsu4qz	297	3	suggest	suggest	VBP
cord-268139-tgpsu4qz	297	4	that	that	IN
cord-268139-tgpsu4qz	297	5	nsp1	nsp1	PRP
cord-268139-tgpsu4qz	297	6	might	may	MD
cord-268139-tgpsu4qz	297	7	play	play	VB
cord-268139-tgpsu4qz	297	8	a	a	DT
cord-268139-tgpsu4qz	297	9	role	role	NN
cord-268139-tgpsu4qz	297	10	in	in	IN
cord-268139-tgpsu4qz	297	11	the	the	DT
cord-268139-tgpsu4qz	297	12	regulation	regulation	NN
cord-268139-tgpsu4qz	297	13	of	of	IN
cord-268139-tgpsu4qz	297	14	total	total	JJ
cord-268139-tgpsu4qz	297	15	viral	viral	JJ
cord-268139-tgpsu4qz	297	16	RNA	RNA	NNP
cord-268139-tgpsu4qz	297	17	amounts	amount	NNS
cord-268139-tgpsu4qz	297	18	rather	rather	RB
cord-268139-tgpsu4qz	297	19	than	than	IN
cord-268139-tgpsu4qz	297	20	in	in	IN
cord-268139-tgpsu4qz	297	21	species	species	NN
cord-268139-tgpsu4qz	297	22	-	-	HYPH
cord-268139-tgpsu4qz	297	23	specific	specific	JJ
cord-268139-tgpsu4qz	297	24	viral	viral	JJ
cord-268139-tgpsu4qz	297	25	RNA	RNA	NNP
cord-268139-tgpsu4qz	297	26	synthesis	synthesis	NN
cord-268139-tgpsu4qz	297	27	.	.	.
cord-268139-tgpsu4qz	298	1	It	-PRON-	PRP
cord-268139-tgpsu4qz	298	2	has	have	VBZ
cord-268139-tgpsu4qz	298	3	been	be	VBN
cord-268139-tgpsu4qz	298	4	previously	previously	RB
cord-268139-tgpsu4qz	298	5	shown	show	VBN
cord-268139-tgpsu4qz	298	6	that	that	IN
cord-268139-tgpsu4qz	298	7	MHV	MHV	NNP
cord-268139-tgpsu4qz	298	8	mutants	mutant	NNS
cord-268139-tgpsu4qz	298	9	unable	unable	JJ
cord-268139-tgpsu4qz	298	10	to	to	TO
cord-268139-tgpsu4qz	298	11	liberate	liberate	VB
cord-268139-tgpsu4qz	298	12	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	298	13	from	from	IN
cord-268139-tgpsu4qz	298	14	the	the	DT
cord-268139-tgpsu4qz	298	15	gene	gene	NN
cord-268139-tgpsu4qz	298	16	1	1	CD
cord-268139-tgpsu4qz	298	17	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	298	18	(	(	-LRB-
cord-268139-tgpsu4qz	298	19	DCS1	dcs1	JJ
cord-268139-tgpsu4qz	298	20	mutants	mutant	NNS
cord-268139-tgpsu4qz	298	21	)	)	-RRB-
cord-268139-tgpsu4qz	298	22	exhibit	exhibit	NN
cord-268139-tgpsu4qz	298	23	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	298	24	very	very	RB
cord-268139-tgpsu4qz	298	25	similar	similar	JJ
cord-268139-tgpsu4qz	298	26	to	to	IN
cord-268139-tgpsu4qz	298	27	the	the	DT
cord-268139-tgpsu4qz	298	28	nsp1	nsp1	NNP
cord-268139-tgpsu4qz	298	29	mutant	mutant	JJ
cord-268139-tgpsu4qz	298	30	viruses	virus	NNS
cord-268139-tgpsu4qz	298	31	described	describe	VBN
cord-268139-tgpsu4qz	298	32	in	in	IN
cord-268139-tgpsu4qz	298	33	this	this	DT
cord-268139-tgpsu4qz	298	34	study	study	NN
cord-268139-tgpsu4qz	298	35	.	.	.
cord-268139-tgpsu4qz	299	1	Because	because	IN
cord-268139-tgpsu4qz	299	2	the	the	DT
cord-268139-tgpsu4qz	299	3	DCS1	DCS1	NNP
cord-268139-tgpsu4qz	299	4	mutations	mutation	NNS
cord-268139-tgpsu4qz	299	5	resulted	result	VBD
cord-268139-tgpsu4qz	299	6	in	in	IN
cord-268139-tgpsu4qz	299	7	a	a	DT
cord-268139-tgpsu4qz	299	8	noncleaved	noncleaved	JJ
cord-268139-tgpsu4qz	299	9	nsp1-nsp2	nsp1-nsp2	NNP
cord-268139-tgpsu4qz	299	10	fusion	fusion	NN
cord-268139-tgpsu4qz	299	11	protein	protein	NN
cord-268139-tgpsu4qz	299	12	,	,	,
cord-268139-tgpsu4qz	299	13	it	-PRON-	PRP
cord-268139-tgpsu4qz	299	14	could	could	MD
cord-268139-tgpsu4qz	299	15	not	not	RB
cord-268139-tgpsu4qz	299	16	be	be	VB
cord-268139-tgpsu4qz	299	17	concluded	conclude	VBN
cord-268139-tgpsu4qz	299	18	that	that	IN
cord-268139-tgpsu4qz	299	19	the	the	DT
cord-268139-tgpsu4qz	299	20	viral	viral	JJ
cord-268139-tgpsu4qz	299	21	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	299	22	were	be	VBD
cord-268139-tgpsu4qz	299	23	solely	solely	RB
cord-268139-tgpsu4qz	299	24	related	relate	VBN
cord-268139-tgpsu4qz	299	25	to	to	IN
cord-268139-tgpsu4qz	299	26	defects	defect	NNS
cord-268139-tgpsu4qz	299	27	in	in	IN
cord-268139-tgpsu4qz	299	28	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	299	29	function	function	NN
cord-268139-tgpsu4qz	299	30	.	.	.
cord-268139-tgpsu4qz	300	1	This	this	DT
cord-268139-tgpsu4qz	300	2	study	study	NN
cord-268139-tgpsu4qz	300	3	corroborates	corroborate	VBZ
cord-268139-tgpsu4qz	300	4	and	and	CC
cord-268139-tgpsu4qz	300	5	extends	extend	VBZ
cord-268139-tgpsu4qz	300	6	these	these	DT
cord-268139-tgpsu4qz	300	7	previous	previous	JJ
cord-268139-tgpsu4qz	300	8	findings	finding	NNS
cord-268139-tgpsu4qz	300	9	and	and	CC
cord-268139-tgpsu4qz	300	10	directly	directly	RB
cord-268139-tgpsu4qz	300	11	correlates	correlate	VBZ
cord-268139-tgpsu4qz	300	12	mutations	mutation	NNS
cord-268139-tgpsu4qz	300	13	in	in	IN
cord-268139-tgpsu4qz	300	14	the	the	DT
cord-268139-tgpsu4qz	300	15	MHV	MHV	NNP
cord-268139-tgpsu4qz	300	16	nsp1-coding	nsp1-coding	NNP
cord-268139-tgpsu4qz	300	17	region	region	NN
cord-268139-tgpsu4qz	300	18	with	with	IN
cord-268139-tgpsu4qz	300	19	changes	change	NNS
cord-268139-tgpsu4qz	300	20	in	in	IN
cord-268139-tgpsu4qz	300	21	viral	viral	JJ
cord-268139-tgpsu4qz	300	22	replication	replication	NN
cord-268139-tgpsu4qz	300	23	and	and	CC
cord-268139-tgpsu4qz	300	24	RNA	RNA	NNP
cord-268139-tgpsu4qz	300	25	synthesis	synthesis	NN
cord-268139-tgpsu4qz	300	26	.	.	.
cord-268139-tgpsu4qz	301	1	The	the	DT
cord-268139-tgpsu4qz	301	2	results	result	NNS
cord-268139-tgpsu4qz	301	3	of	of	IN
cord-268139-tgpsu4qz	301	4	this	this	DT
cord-268139-tgpsu4qz	301	5	study	study	NN
cord-268139-tgpsu4qz	301	6	raise	raise	VB
cord-268139-tgpsu4qz	301	7	important	important	JJ
cord-268139-tgpsu4qz	301	8	questions	question	NNS
cord-268139-tgpsu4qz	301	9	regarding	regard	VBG
cord-268139-tgpsu4qz	301	10	the	the	DT
cord-268139-tgpsu4qz	301	11	mechanistic	mechanistic	JJ
cord-268139-tgpsu4qz	301	12	basis	basis	NN
cord-268139-tgpsu4qz	301	13	for	for	IN
cord-268139-tgpsu4qz	301	14	the	the	DT
cord-268139-tgpsu4qz	301	15	described	describe	VBN
cord-268139-tgpsu4qz	301	16	replication	replication	NN
cord-268139-tgpsu4qz	301	17	and	and	CC
cord-268139-tgpsu4qz	301	18	RNA	RNA	NNP
cord-268139-tgpsu4qz	301	19	synthesis	synthesis	NN
cord-268139-tgpsu4qz	301	20	defects	defect	NNS
cord-268139-tgpsu4qz	301	21	with	with	IN
cord-268139-tgpsu4qz	301	22	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	301	23	mutants	mutant	NNS
cord-268139-tgpsu4qz	301	24	.	.	.
cord-268139-tgpsu4qz	302	1	Why	why	WRB
cord-268139-tgpsu4qz	302	2	is	be	VBZ
cord-268139-tgpsu4qz	302	3	the	the	DT
cord-268139-tgpsu4qz	302	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	302	5	amino	amino	NN
cord-268139-tgpsu4qz	302	6	-	-	HYPH
cord-268139-tgpsu4qz	302	7	terminus	terminus	NN
cord-268139-tgpsu4qz	302	8	required	require	VBN
cord-268139-tgpsu4qz	302	9	for	for	IN
cord-268139-tgpsu4qz	302	10	MHV	MHV	NNP
cord-268139-tgpsu4qz	302	11	replication	replication	NN
cord-268139-tgpsu4qz	302	12	?	?	.
cord-268139-tgpsu4qz	303	1	What	what	WP
cord-268139-tgpsu4qz	303	2	function	function	NN
cord-268139-tgpsu4qz	303	3	does	do	VBZ
cord-268139-tgpsu4qz	303	4	this	this	DT
cord-268139-tgpsu4qz	303	5	domain	domain	NN
cord-268139-tgpsu4qz	303	6	have	have	VB
cord-268139-tgpsu4qz	303	7	during	during	IN
cord-268139-tgpsu4qz	303	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	303	9	RNA	RNA	NNP
cord-268139-tgpsu4qz	303	10	synthesis	synthesis	NN
cord-268139-tgpsu4qz	303	11	?	?	.
cord-268139-tgpsu4qz	304	1	Do	do	VB
cord-268139-tgpsu4qz	304	2	the	the	DT
cord-268139-tgpsu4qz	304	3	deletion	deletion	NN
cord-268139-tgpsu4qz	304	4	and	and	CC
cord-268139-tgpsu4qz	304	5	point	point	NN
cord-268139-tgpsu4qz	304	6	mutations	mutation	NNS
cord-268139-tgpsu4qz	304	7	change	change	VBP
cord-268139-tgpsu4qz	304	8	the	the	DT
cord-268139-tgpsu4qz	304	9	nature	nature	NN
cord-268139-tgpsu4qz	304	10	of	of	IN
cord-268139-tgpsu4qz	304	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	304	12	-protein	-protein	WDT
cord-268139-tgpsu4qz	304	13	interactions	interaction	NNS
cord-268139-tgpsu4qz	304	14	?	?	.
cord-268139-tgpsu4qz	305	1	Yeast	yeast	NN
cord-268139-tgpsu4qz	305	2	two	two	CD
cord-268139-tgpsu4qz	305	3	-	-	HYPH
cord-268139-tgpsu4qz	305	4	hybrid	hybrid	NN
cord-268139-tgpsu4qz	305	5	and	and	CC
cord-268139-tgpsu4qz	305	6	co	co	JJ
cord-268139-tgpsu4qz	305	7	-	-	JJ
cord-268139-tgpsu4qz	305	8	immunoprecipitation	immunoprecipitation	JJ
cord-268139-tgpsu4qz	305	9	assays	assay	NNS
cord-268139-tgpsu4qz	305	10	previously	previously	RB
cord-268139-tgpsu4qz	305	11	demonstrated	demonstrate	VBD
cord-268139-tgpsu4qz	305	12	that	that	IN
cord-268139-tgpsu4qz	305	13	wild	wild	JJ
cord-268139-tgpsu4qz	305	14	-	-	HYPH
cord-268139-tgpsu4qz	305	15	type	type	NN
cord-268139-tgpsu4qz	305	16	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	305	17	directly	directly	RB
cord-268139-tgpsu4qz	305	18	binds	bind	VBZ
cord-268139-tgpsu4qz	305	19	to	to	IN
cord-268139-tgpsu4qz	305	20	nsp7	nsp7	NN
cord-268139-tgpsu4qz	305	21	and	and	CC
cord-268139-tgpsu4qz	305	22	nsp10	nsp10	NNPS
cord-268139-tgpsu4qz	305	23	,	,	,
cord-268139-tgpsu4qz	305	24	two	two	CD
cord-268139-tgpsu4qz	305	25	other	other	JJ
cord-268139-tgpsu4qz	305	26	gene	gene	NN
cord-268139-tgpsu4qz	305	27	1	1	CD
cord-268139-tgpsu4qz	305	28	proteins	protein	NNS
cord-268139-tgpsu4qz	305	29	.	.	.
cord-268139-tgpsu4qz	306	1	The	the	DT
cord-268139-tgpsu4qz	306	2	interactions	interaction	NNS
cord-268139-tgpsu4qz	306	3	between	between	IN
cord-268139-tgpsu4qz	306	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	306	5	,	,	,
cord-268139-tgpsu4qz	306	6	nsp7	nsp7	UH
cord-268139-tgpsu4qz	306	7	,	,	,
cord-268139-tgpsu4qz	306	8	and	and	CC
cord-268139-tgpsu4qz	306	9	nsp10	nsp10	NNPS
cord-268139-tgpsu4qz	306	10	do	do	VBP
cord-268139-tgpsu4qz	306	11	not	not	RB
cord-268139-tgpsu4qz	306	12	require	require	VB
cord-268139-tgpsu4qz	306	13	the	the	DT
cord-268139-tgpsu4qz	306	14	nsp1	nsp1	NNP
cord-268139-tgpsu4qz	306	15	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	306	16	-	-	HYPH
cord-268139-tgpsu4qz	306	17	terminus	terminus	NN
cord-268139-tgpsu4qz	306	18	(	(	-LRB-
cord-268139-tgpsu4qz	306	19	F	F	NNP
cord-268139-tgpsu4qz	306	20	167	167	CD
cord-268139-tgpsu4qz	306	21	through	through	IN
cord-268139-tgpsu4qz	306	22	G	G	NNP
cord-268139-tgpsu4qz	306	23	247	247	CD
cord-268139-tgpsu4qz	306	24	)	)	-RRB-
cord-268139-tgpsu4qz	306	25	but	but	CC
cord-268139-tgpsu4qz	306	26	do	do	VBP
cord-268139-tgpsu4qz	306	27	require	require	VB
cord-268139-tgpsu4qz	306	28	residues	residue	NNS
cord-268139-tgpsu4qz	306	29	amino	amino	NN
cord-268139-tgpsu4qz	306	30	-	-	HYPH
cord-268139-tgpsu4qz	306	31	terminal	terminal	NN
cord-268139-tgpsu4qz	306	32	to	to	IN
cord-268139-tgpsu4qz	306	33	F	F	NNP
cord-268139-tgpsu4qz	306	34	167	167	CD
cord-268139-tgpsu4qz	306	35	(	(	-LRB-
cord-268139-tgpsu4qz	306	36	i.e.	i.e.	FW
cord-268139-tgpsu4qz	306	37	,	,	,
cord-268139-tgpsu4qz	306	38	residues	residue	NNS
cord-268139-tgpsu4qz	306	39	E	E	NNP
cord-268139-tgpsu4qz	306	40	84	84	CD
cord-268139-tgpsu4qz	306	41	through	through	IN
cord-268139-tgpsu4qz	306	42	R	R	NNP
cord-268139-tgpsu4qz	306	43	166	166	CD
cord-268139-tgpsu4qz	306	44	)	)	-RRB-
cord-268139-tgpsu4qz	306	45	.	.	.
cord-268139-tgpsu4qz	307	1	Because	because	IN
cord-268139-tgpsu4qz	307	2	the	the	DT
cord-268139-tgpsu4qz	307	3	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	307	4	virus	virus	NN
cord-268139-tgpsu4qz	307	5	was	be	VBD
cord-268139-tgpsu4qz	307	6	engineered	engineer	VBN
cord-268139-tgpsu4qz	307	7	to	to	TO
cord-268139-tgpsu4qz	307	8	lack	lack	VB
cord-268139-tgpsu4qz	307	9	this	this	DT
cord-268139-tgpsu4qz	307	10	putative	putative	JJ
cord-268139-tgpsu4qz	307	11	interaction	interaction	NN
cord-268139-tgpsu4qz	307	12	domain	domain	NN
cord-268139-tgpsu4qz	307	13	,	,	,
cord-268139-tgpsu4qz	307	14	it	-PRON-	PRP
cord-268139-tgpsu4qz	307	15	is	be	VBZ
cord-268139-tgpsu4qz	307	16	interesting	interesting	JJ
cord-268139-tgpsu4qz	307	17	to	to	TO
cord-268139-tgpsu4qz	307	18	speculate	speculate	VB
cord-268139-tgpsu4qz	307	19	whether	whether	IN
cord-268139-tgpsu4qz	307	20	the	the	DT
cord-268139-tgpsu4qz	307	21	lack	lack	NN
cord-268139-tgpsu4qz	307	22	of	of	IN
cord-268139-tgpsu4qz	307	23	viability	viability	NN
cord-268139-tgpsu4qz	307	24	for	for	IN
cord-268139-tgpsu4qz	307	25	this	this	DT
cord-268139-tgpsu4qz	307	26	mutant	mutant	NN
cord-268139-tgpsu4qz	307	27	correlates	correlate	VBZ
cord-268139-tgpsu4qz	307	28	with	with	IN
cord-268139-tgpsu4qz	307	29	a	a	DT
cord-268139-tgpsu4qz	307	30	loss	loss	NN
cord-268139-tgpsu4qz	307	31	of	of	IN
cord-268139-tgpsu4qz	307	32	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	307	33	binding	bind	VBG
cord-268139-tgpsu4qz	307	34	to	to	IN
cord-268139-tgpsu4qz	307	35	either	either	CC
cord-268139-tgpsu4qz	307	36	nsp7	nsp7	NN
cord-268139-tgpsu4qz	307	37	and/or	and/or	CC
cord-268139-tgpsu4qz	307	38	nsp10	nsp10	NNP
cord-268139-tgpsu4qz	307	39	.	.	.
cord-268139-tgpsu4qz	308	1	Furthermore	furthermore	RB
cord-268139-tgpsu4qz	308	2	,	,	,
cord-268139-tgpsu4qz	308	3	it	-PRON-	PRP
cord-268139-tgpsu4qz	308	4	is	be	VBZ
cord-268139-tgpsu4qz	308	5	thought	think	VBN
cord-268139-tgpsu4qz	308	6	that	that	IN
cord-268139-tgpsu4qz	308	7	charge	charge	NN
cord-268139-tgpsu4qz	308	8	-	-	HYPH
cord-268139-tgpsu4qz	308	9	to	to	IN
cord-268139-tgpsu4qz	308	10	-	-	HYPH
cord-268139-tgpsu4qz	308	11	alanine	alanine	NN
cord-268139-tgpsu4qz	308	12	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	308	13	alters	alter	VBZ
cord-268139-tgpsu4qz	308	14	a	a	DT
cord-268139-tgpsu4qz	308	15	protein	protein	NN
cord-268139-tgpsu4qz	308	16	's	's	POS
cord-268139-tgpsu4qz	308	17	capacity	capacity	NN
cord-268139-tgpsu4qz	308	18	to	to	TO
cord-268139-tgpsu4qz	308	19	participate	participate	VB
cord-268139-tgpsu4qz	308	20	in	in	IN
cord-268139-tgpsu4qz	308	21	interand	interand	NN
cord-268139-tgpsu4qz	308	22	intra	intra	JJ
cord-268139-tgpsu4qz	308	23	-	-	JJ
cord-268139-tgpsu4qz	308	24	molecular	molecular	JJ
cord-268139-tgpsu4qz	308	25	interactions	interaction	NNS
cord-268139-tgpsu4qz	308	26	(	(	-LRB-
cord-268139-tgpsu4qz	308	27	Cunningham	Cunningham	NNP
cord-268139-tgpsu4qz	308	28	and	and	CC
cord-268139-tgpsu4qz	308	29	Wells	Wells	NNP
cord-268139-tgpsu4qz	308	30	,	,	,
cord-268139-tgpsu4qz	308	31	1989	1989	CD
cord-268139-tgpsu4qz	308	32	)	)	-RRB-
cord-268139-tgpsu4qz	308	33	.	.	.
cord-268139-tgpsu4qz	309	1	This	this	DT
cord-268139-tgpsu4qz	309	2	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	309	3	approach	approach	NN
cord-268139-tgpsu4qz	309	4	is	be	VBZ
cord-268139-tgpsu4qz	309	5	supposed	suppose	VBN
cord-268139-tgpsu4qz	309	6	to	to	TO
cord-268139-tgpsu4qz	309	7	minimize	minimize	VB
cord-268139-tgpsu4qz	309	8	disruption	disruption	NN
cord-268139-tgpsu4qz	309	9	of	of	IN
cord-268139-tgpsu4qz	309	10	protein	protein	NN
cord-268139-tgpsu4qz	309	11	secondary	secondary	JJ
cord-268139-tgpsu4qz	309	12	structure	structure	NN
cord-268139-tgpsu4qz	309	13	;	;	:
cord-268139-tgpsu4qz	309	14	however	however	RB
cord-268139-tgpsu4qz	309	15	,	,	,
cord-268139-tgpsu4qz	309	16	it	-PRON-	PRP
cord-268139-tgpsu4qz	309	17	is	be	VBZ
cord-268139-tgpsu4qz	309	18	possible	possible	JJ
cord-268139-tgpsu4qz	309	19	that	that	IN
cord-268139-tgpsu4qz	309	20	some	some	DT
cord-268139-tgpsu4qz	309	21	of	of	IN
cord-268139-tgpsu4qz	309	22	the	the	DT
cord-268139-tgpsu4qz	309	23	charge	charge	NN
cord-268139-tgpsu4qz	309	24	-	-	HYPH
cord-268139-tgpsu4qz	309	25	to	to	IN
cord-268139-tgpsu4qz	309	26	-	-	HYPH
cord-268139-tgpsu4qz	309	27	alanine	alanine	NN
cord-268139-tgpsu4qz	309	28	mutations	mutation	NNS
cord-268139-tgpsu4qz	309	29	caused	cause	VBD
cord-268139-tgpsu4qz	309	30	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	309	31	to	to	TO
cord-268139-tgpsu4qz	309	32	misfold	misfold	VB
cord-268139-tgpsu4qz	309	33	.	.	.
cord-268139-tgpsu4qz	310	1	In	in	IN
cord-268139-tgpsu4qz	310	2	support	support	NN
cord-268139-tgpsu4qz	310	3	of	of	IN
cord-268139-tgpsu4qz	310	4	this	this	DT
cord-268139-tgpsu4qz	310	5	possibility	possibility	NN
cord-268139-tgpsu4qz	310	6	,	,	,
cord-268139-tgpsu4qz	310	7	VUSB1-nsp1	VUSB1-nsp1	NNP
cord-268139-tgpsu4qz	310	8	was	be	VBD
cord-268139-tgpsu4qz	310	9	detected	detect	VBN
cord-268139-tgpsu4qz	310	10	by	by	IN
cord-268139-tgpsu4qz	310	11	a	a	DT
cord-268139-tgpsu4qz	310	12	-	-	HYPH
cord-268139-tgpsu4qz	310	13	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	310	14	antisera	antisera	NN
cord-268139-tgpsu4qz	310	15	during	during	IN
cord-268139-tgpsu4qz	310	16	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	310	17	assays	assay	NNS
cord-268139-tgpsu4qz	310	18	but	but	CC
cord-268139-tgpsu4qz	310	19	not	not	RB
cord-268139-tgpsu4qz	310	20	during	during	IN
cord-268139-tgpsu4qz	310	21	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	310	22	assays	assay	NNS
cord-268139-tgpsu4qz	310	23	,	,	,
cord-268139-tgpsu4qz	310	24	suggesting	suggest	VBG
cord-268139-tgpsu4qz	310	25	that	that	IN
cord-268139-tgpsu4qz	310	26	this	this	DT
cord-268139-tgpsu4qz	310	27	mutant	mutant	JJ
cord-268139-tgpsu4qz	310	28	protein	protein	NN
cord-268139-tgpsu4qz	310	29	may	may	MD
cord-268139-tgpsu4qz	310	30	have	have	VB
cord-268139-tgpsu4qz	310	31	a	a	DT
cord-268139-tgpsu4qz	310	32	different	different	JJ
cord-268139-tgpsu4qz	310	33	structural	structural	JJ
cord-268139-tgpsu4qz	310	34	conformation	conformation	NN
cord-268139-tgpsu4qz	310	35	.	.	.
cord-268139-tgpsu4qz	311	1	Regardless	regardless	RB
cord-268139-tgpsu4qz	311	2	,	,	,
cord-268139-tgpsu4qz	311	3	the	the	DT
cord-268139-tgpsu4qz	311	4	introduced	introduce	VBN
cord-268139-tgpsu4qz	311	5	mutations	mutation	NNS
cord-268139-tgpsu4qz	311	6	may	may	MD
cord-268139-tgpsu4qz	311	7	have	have	VB
cord-268139-tgpsu4qz	311	8	altered	alter	VBN
cord-268139-tgpsu4qz	311	9	the	the	DT
cord-268139-tgpsu4qz	311	10	capacity	capacity	NN
cord-268139-tgpsu4qz	311	11	of	of	IN
cord-268139-tgpsu4qz	311	12	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	311	13	to	to	TO
cord-268139-tgpsu4qz	311	14	bind	bind	VB
cord-268139-tgpsu4qz	311	15	to	to	IN
cord-268139-tgpsu4qz	311	16	nsp7	nsp7	NNP
cord-268139-tgpsu4qz	311	17	,	,	,
cord-268139-tgpsu4qz	311	18	nsp10	nsp10	NNPS
cord-268139-tgpsu4qz	311	19	,	,	,
cord-268139-tgpsu4qz	311	20	or	or	CC
cord-268139-tgpsu4qz	311	21	other	other	JJ
cord-268139-tgpsu4qz	311	22	proteins	protein	NNS
cord-268139-tgpsu4qz	311	23	resulting	result	VBG
cord-268139-tgpsu4qz	311	24	in	in	IN
cord-268139-tgpsu4qz	311	25	viral	viral	JJ
cord-268139-tgpsu4qz	311	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	311	27	synthesis	synthesis	NN
cord-268139-tgpsu4qz	311	28	and	and	CC
cord-268139-tgpsu4qz	311	29	viral	viral	JJ
cord-268139-tgpsu4qz	311	30	replication	replication	NN
cord-268139-tgpsu4qz	311	31	defects	defect	NNS
cord-268139-tgpsu4qz	311	32	.	.	.
cord-268139-tgpsu4qz	312	1	During	during	IN
cord-268139-tgpsu4qz	312	2	wild	wild	JJ
cord-268139-tgpsu4qz	312	3	-	-	HYPH
cord-268139-tgpsu4qz	312	4	type	type	NN
cord-268139-tgpsu4qz	312	5	MHV	MHV	NNP
cord-268139-tgpsu4qz	312	6	infection	infection	NN
cord-268139-tgpsu4qz	312	7	,	,	,
cord-268139-tgpsu4qz	312	8	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	312	9	,	,	,
cord-268139-tgpsu4qz	312	10	nsp2	nsp2	CC
cord-268139-tgpsu4qz	312	11	,	,	,
cord-268139-tgpsu4qz	312	12	and	and	CC
cord-268139-tgpsu4qz	312	13	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	312	14	are	be	VBP
cord-268139-tgpsu4qz	312	15	processed	process	VBN
cord-268139-tgpsu4qz	312	16	in	in	IN
cord-268139-tgpsu4qz	312	17	an	an	DT
cord-268139-tgpsu4qz	312	18	ordered	order	VBN
cord-268139-tgpsu4qz	312	19	manner	manner	NN
cord-268139-tgpsu4qz	312	20	(	(	-LRB-
cord-268139-tgpsu4qz	312	21	Fig	Fig	NNP
cord-268139-tgpsu4qz	312	22	.	.	.
cord-268139-tgpsu4qz	312	23	7A	7a	NN
cord-268139-tgpsu4qz	312	24	)	)	-RRB-
cord-268139-tgpsu4qz	312	25	.	.	.
cord-268139-tgpsu4qz	313	1	Immediately	immediately	RB
cord-268139-tgpsu4qz	313	2	following	follow	VBG
cord-268139-tgpsu4qz	313	3	translation	translation	NN
cord-268139-tgpsu4qz	313	4	of	of	IN
cord-268139-tgpsu4qz	313	5	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	313	6	within	within	IN
cord-268139-tgpsu4qz	313	7	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	313	8	,	,	,
cord-268139-tgpsu4qz	313	9	CS1	CS1	NNP
cord-268139-tgpsu4qz	313	10	is	be	VBZ
cord-268139-tgpsu4qz	313	11	rapidly	rapidly	RB
cord-268139-tgpsu4qz	313	12	cleaved	cleave	VBN
cord-268139-tgpsu4qz	313	13	,	,	,
cord-268139-tgpsu4qz	313	14	releasing	release	VBG
cord-268139-tgpsu4qz	313	15	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	313	16	as	as	IN
cord-268139-tgpsu4qz	313	17	polyprotein	polyprotein	NNP
cord-268139-tgpsu4qz	313	18	synthesis	synthesis	NNP
cord-268139-tgpsu4qz	313	19	continues	continue	VBZ
cord-268139-tgpsu4qz	313	20	(	(	-LRB-
cord-268139-tgpsu4qz	314	1	Denison	Denison	NNP
cord-268139-tgpsu4qz	314	2	et	et	NNP
cord-268139-tgpsu4qz	314	3	al	al	NNP
cord-268139-tgpsu4qz	314	4	.	.	.
cord-268139-tgpsu4qz	315	1	,	,	,
cord-268139-tgpsu4qz	315	2	1992	1992	CD
cord-268139-tgpsu4qz	316	1	Harcourt	Harcourt	NNP
cord-268139-tgpsu4qz	316	2	et	et	NNP
cord-268139-tgpsu4qz	316	3	al	al	NNP
cord-268139-tgpsu4qz	316	4	.	.	NNP
cord-268139-tgpsu4qz	316	5	,	,	,
cord-268139-tgpsu4qz	316	6	2004	2004	CD
cord-268139-tgpsu4qz	317	1	;	;	:
cord-268139-tgpsu4qz	317	2	Schiller	Schiller	NNP
cord-268139-tgpsu4qz	317	3	et	et	NNP
cord-268139-tgpsu4qz	317	4	al	al	NNP
cord-268139-tgpsu4qz	317	5	.	.	NNP
cord-268139-tgpsu4qz	317	6	,	,	,
cord-268139-tgpsu4qz	317	7	1998	1998	CD
cord-268139-tgpsu4qz	317	8	)	)	-RRB-
cord-268139-tgpsu4qz	317	9	.	.	.
cord-268139-tgpsu4qz	318	1	Next	next	RB
cord-268139-tgpsu4qz	318	2	,	,	,
cord-268139-tgpsu4qz	318	3	cleavage	cleavage	NN
cord-268139-tgpsu4qz	318	4	site	site	NN
cord-268139-tgpsu4qz	318	5	3	3	CD
cord-268139-tgpsu4qz	318	6	(	(	-LRB-
cord-268139-tgpsu4qz	318	7	CS3	CS3	NNP
cord-268139-tgpsu4qz	318	8	)	)	-RRB-
cord-268139-tgpsu4qz	318	9	is	be	VBZ
cord-268139-tgpsu4qz	318	10	cleaved	cleave	VBN
cord-268139-tgpsu4qz	318	11	(	(	-LRB-
cord-268139-tgpsu4qz	318	12	likely	likely	JJ
cord-268139-tgpsu4qz	318	13	by	by	IN
cord-268139-tgpsu4qz	318	14	PLP2	PLP2	NNP
cord-268139-tgpsu4qz	318	15	)	)	-RRB-
cord-268139-tgpsu4qz	318	16	liberating	liberate	VBG
cord-268139-tgpsu4qz	318	17	an	an	DT
cord-268139-tgpsu4qz	318	18	nsp2	nsp2	NN
cord-268139-tgpsu4qz	318	19	-nsp3	-nsp3	NNP
cord-268139-tgpsu4qz	318	20	precursor	precursor	NN
cord-268139-tgpsu4qz	318	21	,	,	,
cord-268139-tgpsu4qz	318	22	which	which	WDT
cord-268139-tgpsu4qz	318	23	is	be	VBZ
cord-268139-tgpsu4qz	318	24	finally	finally	RB
cord-268139-tgpsu4qz	318	25	processed	process	VBN
cord-268139-tgpsu4qz	318	26	at	at	IN
cord-268139-tgpsu4qz	318	27	cleavage	cleavage	NN
cord-268139-tgpsu4qz	318	28	site	site	NN
cord-268139-tgpsu4qz	318	29	2	2	CD
cord-268139-tgpsu4qz	318	30	(	(	-LRB-
cord-268139-tgpsu4qz	318	31	CS2	CS2	NNP
cord-268139-tgpsu4qz	318	32	)	)	-RRB-
cord-268139-tgpsu4qz	318	33	to	to	TO
cord-268139-tgpsu4qz	318	34	release	release	VB
cord-268139-tgpsu4qz	318	35	mature	mature	NN
cord-268139-tgpsu4qz	318	36	nsp2	nsp2	NNS
cord-268139-tgpsu4qz	318	37	and	and	CC
cord-268139-tgpsu4qz	318	38	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	318	39	products	product	NNS
cord-268139-tgpsu4qz	318	40	(	(	-LRB-
cord-268139-tgpsu4qz	318	41	Denison	Denison	NNP
cord-268139-tgpsu4qz	318	42	et	et	NNP
cord-268139-tgpsu4qz	318	43	al	al	NNP
cord-268139-tgpsu4qz	318	44	.	.	NNP
cord-268139-tgpsu4qz	318	45	,	,	,
cord-268139-tgpsu4qz	318	46	1992	1992	CD
cord-268139-tgpsu4qz	319	1	Harcourt	Harcourt	NNP
cord-268139-tgpsu4qz	319	2	et	et	NNP
cord-268139-tgpsu4qz	319	3	al	al	NNP
cord-268139-tgpsu4qz	319	4	.	.	NNP
cord-268139-tgpsu4qz	319	5	,	,	,
cord-268139-tgpsu4qz	319	6	2004	2004	CD
cord-268139-tgpsu4qz	320	1	;	;	:
cord-268139-tgpsu4qz	320	2	Schiller	Schiller	NNP
cord-268139-tgpsu4qz	320	3	et	et	NNP
cord-268139-tgpsu4qz	320	4	al	al	NNP
cord-268139-tgpsu4qz	320	5	.	.	NNP
cord-268139-tgpsu4qz	320	6	,	,	,
cord-268139-tgpsu4qz	320	7	1998	1998	CD
cord-268139-tgpsu4qz	320	8	)	)	-RRB-
cord-268139-tgpsu4qz	320	9	.	.	.
cord-268139-tgpsu4qz	321	1	In	in	IN
cord-268139-tgpsu4qz	321	2	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	321	3	virus	virus	NN
cord-268139-tgpsu4qz	321	4	-	-	HYPH
cord-268139-tgpsu4qz	321	5	infected	infect	VBN
cord-268139-tgpsu4qz	321	6	cells	cell	NNS
cord-268139-tgpsu4qz	321	7	,	,	,
cord-268139-tgpsu4qz	321	8	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	321	9	(	(	-LRB-
cord-268139-tgpsu4qz	321	10	14-kDa	14-kda	CD
cord-268139-tgpsu4qz	321	11	)	)	-RRB-
cord-268139-tgpsu4qz	321	12	and	and	CC
cord-268139-tgpsu4qz	321	13	nsp2	nsp2	NN
cord-268139-tgpsu4qz	321	14	are	be	VBP
cord-268139-tgpsu4qz	321	15	present	present	JJ
cord-268139-tgpsu4qz	321	16	,	,	,
cord-268139-tgpsu4qz	321	17	demonstrating	demonstrate	VBG
cord-268139-tgpsu4qz	321	18	that	that	IN
cord-268139-tgpsu4qz	321	19	the	the	DT
cord-268139-tgpsu4qz	321	20	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	321	21	-	-	HYPH
cord-268139-tgpsu4qz	321	22	terminal	terminal	NN
cord-268139-tgpsu4qz	321	23	half	half	NN
cord-268139-tgpsu4qz	321	24	of	of	IN
cord-268139-tgpsu4qz	321	25	nsp1	nsp1	CD
cord-268139-tgpsu4qz	321	26	is	be	VBZ
cord-268139-tgpsu4qz	321	27	not	not	RB
cord-268139-tgpsu4qz	321	28	absolutely	absolutely	RB
cord-268139-tgpsu4qz	321	29	essential	essential	JJ
cord-268139-tgpsu4qz	321	30	for	for	IN
cord-268139-tgpsu4qz	321	31	cleavage	cleavage	NN
cord-268139-tgpsu4qz	321	32	at	at	IN
cord-268139-tgpsu4qz	321	33	either	either	CC
cord-268139-tgpsu4qz	321	34	CS1	CS1	NNP
cord-268139-tgpsu4qz	321	35	or	or	CC
cord-268139-tgpsu4qz	321	36	CS2	CS2	NNP
cord-268139-tgpsu4qz	321	37	.	.	.
cord-268139-tgpsu4qz	322	1	However	however	RB
cord-268139-tgpsu4qz	322	2	,	,	,
cord-268139-tgpsu4qz	322	3	the	the	DT
cord-268139-tgpsu4qz	322	4	identification	identification	NN
cord-268139-tgpsu4qz	322	5	of	of	IN
cord-268139-tgpsu4qz	322	6	an	an	DT
cord-268139-tgpsu4qz	322	7	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	322	8	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	322	9	-nsp2	-nsp2	NNP
cord-268139-tgpsu4qz	322	10	polypeptide	polypeptide	NN
cord-268139-tgpsu4qz	322	11	in	in	IN
cord-268139-tgpsu4qz	322	12	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	322	13	virus	virus	NN
cord-268139-tgpsu4qz	322	14	-	-	HYPH
cord-268139-tgpsu4qz	322	15	infected	infect	VBN
cord-268139-tgpsu4qz	322	16	cells	cell	NNS
cord-268139-tgpsu4qz	322	17	reveals	reveal	VBZ
cord-268139-tgpsu4qz	322	18	that	that	IN
cord-268139-tgpsu4qz	322	19	this	this	DT
cord-268139-tgpsu4qz	322	20	region	region	NN
cord-268139-tgpsu4qz	322	21	of	of	IN
cord-268139-tgpsu4qz	322	22	the	the	DT
cord-268139-tgpsu4qz	322	23	protein	protein	NN
cord-268139-tgpsu4qz	322	24	(	(	-LRB-
cord-268139-tgpsu4qz	322	25	residues	residue	NNS
cord-268139-tgpsu4qz	322	26	K	K	NNP
cord-268139-tgpsu4qz	322	27	124	124	CD
cord-268139-tgpsu4qz	322	28	through	through	IN
cord-268139-tgpsu4qz	322	29	L	L	NNP
cord-268139-tgpsu4qz	322	30	241	241	CD
cord-268139-tgpsu4qz	322	31	)	)	-RRB-
cord-268139-tgpsu4qz	322	32	is	be	VBZ
cord-268139-tgpsu4qz	322	33	important	important	JJ
cord-268139-tgpsu4qz	322	34	for	for	IN
cord-268139-tgpsu4qz	322	35	efficient	efficient	JJ
cord-268139-tgpsu4qz	322	36	CS1	CS1	NNP
cord-268139-tgpsu4qz	322	37	processing	processing	NN
cord-268139-tgpsu4qz	322	38	.	.	.
cord-268139-tgpsu4qz	323	1	Fig	Fig	NNP
cord-268139-tgpsu4qz	323	2	.	.	NNP
cord-268139-tgpsu4qz	323	3	7	7	CD
cord-268139-tgpsu4qz	323	4	.	.	.
cord-268139-tgpsu4qz	324	1	Model	Model	NNP
cord-268139-tgpsu4qz	324	2	of	of	IN
cord-268139-tgpsu4qz	324	3	icwt	icwt	NNP
cord-268139-tgpsu4qz	324	4	and	and	CC
cord-268139-tgpsu4qz	324	5	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	324	6	virus	virus	NN
cord-268139-tgpsu4qz	324	7	protein	protein	NN
cord-268139-tgpsu4qz	324	8	processing	processing	NN
cord-268139-tgpsu4qz	324	9	.	.	.
cord-268139-tgpsu4qz	325	1	Shown	show	VBN
cord-268139-tgpsu4qz	325	2	are	be	VBP
cord-268139-tgpsu4qz	325	3	schematics	schematic	NNS
cord-268139-tgpsu4qz	325	4	of	of	IN
cord-268139-tgpsu4qz	325	5	wild	wild	JJ
cord-268139-tgpsu4qz	325	6	-	-	HYPH
cord-268139-tgpsu4qz	325	7	type	type	NN
cord-268139-tgpsu4qz	325	8	and	and	CC
cord-268139-tgpsu4qz	325	9	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	325	10	virus	virus	NN
cord-268139-tgpsu4qz	325	11	amino	amino	NN
cord-268139-tgpsu4qz	325	12	-	-	HYPH
cord-268139-tgpsu4qz	325	13	terminal	terminal	NN
cord-268139-tgpsu4qz	325	14	gene	gene	NN
cord-268139-tgpsu4qz	325	15	1	1	CD
cord-268139-tgpsu4qz	325	16	nsps	nsp	NNS
cord-268139-tgpsu4qz	325	17	(	(	-LRB-
cord-268139-tgpsu4qz	325	18	boxes	box	NNS
cord-268139-tgpsu4qz	325	19	)	)	-RRB-
cord-268139-tgpsu4qz	325	20	.	.	.
cord-268139-tgpsu4qz	326	1	PLP1	PLP1	NNP
cord-268139-tgpsu4qz	326	2	and	and	CC
cord-268139-tgpsu4qz	326	3	PLP2	PLP2	NNP
cord-268139-tgpsu4qz	326	4	are	be	VBP
cord-268139-tgpsu4qz	326	5	shown	show	VBN
cord-268139-tgpsu4qz	326	6	as	as	IN
cord-268139-tgpsu4qz	326	7	gray	gray	JJ
cord-268139-tgpsu4qz	326	8	boxes	box	NNS
cord-268139-tgpsu4qz	326	9	within	within	IN
cord-268139-tgpsu4qz	326	10	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	326	11	.	.	.
cord-268139-tgpsu4qz	327	1	The	the	DT
cord-268139-tgpsu4qz	327	2	individual	individual	JJ
cord-268139-tgpsu4qz	327	3	cleavage	cleavage	NN
cord-268139-tgpsu4qz	327	4	sites	site	NNS
cord-268139-tgpsu4qz	327	5	are	be	VBP
cord-268139-tgpsu4qz	327	6	labeled	label	VBN
cord-268139-tgpsu4qz	327	7	,	,	,
cord-268139-tgpsu4qz	327	8	and	and	CC
cord-268139-tgpsu4qz	327	9	the	the	DT
cord-268139-tgpsu4qz	327	10	order	order	NN
cord-268139-tgpsu4qz	327	11	of	of	IN
cord-268139-tgpsu4qz	327	12	processing	processing	NN
cord-268139-tgpsu4qz	327	13	is	be	VBZ
cord-268139-tgpsu4qz	327	14	indicated	indicate	VBN
cord-268139-tgpsu4qz	327	15	above	above	IN
cord-268139-tgpsu4qz	327	16	the	the	DT
cord-268139-tgpsu4qz	327	17	closed	closed	JJ
cord-268139-tgpsu4qz	327	18	arrowheads	arrowhead	NNS
cord-268139-tgpsu4qz	327	19	.	.	.
cord-268139-tgpsu4qz	328	1	Lines	line	NNS
cord-268139-tgpsu4qz	328	2	below	below	IN
cord-268139-tgpsu4qz	328	3	the	the	DT
cord-268139-tgpsu4qz	328	4	protein	protein	NN
cord-268139-tgpsu4qz	328	5	schematics	schematic	NNS
cord-268139-tgpsu4qz	328	6	illustrate	illustrate	VBP
cord-268139-tgpsu4qz	328	7	the	the	DT
cord-268139-tgpsu4qz	328	8	size	size	NN
cord-268139-tgpsu4qz	328	9	of	of	IN
cord-268139-tgpsu4qz	328	10	mature	mature	JJ
cord-268139-tgpsu4qz	328	11	proteins	protein	NNS
cord-268139-tgpsu4qz	328	12	following	follow	VBG
cord-268139-tgpsu4qz	328	13	cleavage	cleavage	NN
cord-268139-tgpsu4qz	328	14	at	at	IN
cord-268139-tgpsu4qz	328	15	individual	individual	JJ
cord-268139-tgpsu4qz	328	16	sites	site	NNS
cord-268139-tgpsu4qz	328	17	.	.	.
cord-268139-tgpsu4qz	329	1	(	(	-LRB-
cord-268139-tgpsu4qz	329	2	A	a	NN
cord-268139-tgpsu4qz	329	3	)	)	-RRB-
cord-268139-tgpsu4qz	329	4	Processing	processing	NN
cord-268139-tgpsu4qz	329	5	of	of	IN
cord-268139-tgpsu4qz	329	6	wild	wild	JJ
cord-268139-tgpsu4qz	329	7	-	-	HYPH
cord-268139-tgpsu4qz	329	8	type	type	NN
cord-268139-tgpsu4qz	329	9	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	329	10	,	,	,
cord-268139-tgpsu4qz	329	11	nsp2	nsp2	CC
cord-268139-tgpsu4qz	329	12	,	,	,
cord-268139-tgpsu4qz	329	13	and	and	CC
cord-268139-tgpsu4qz	329	14	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	329	15	.	.	.
cord-268139-tgpsu4qz	330	1	During	during	IN
cord-268139-tgpsu4qz	330	2	infection	infection	NN
cord-268139-tgpsu4qz	330	3	,	,	,
cord-268139-tgpsu4qz	330	4	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	330	5	(	(	-LRB-
cord-268139-tgpsu4qz	330	6	28	28	CD
cord-268139-tgpsu4qz	330	7	kDa	kDa	NNPS
cord-268139-tgpsu4qz	330	8	)	)	-RRB-
cord-268139-tgpsu4qz	330	9	is	be	VBZ
cord-268139-tgpsu4qz	330	10	cleaved	cleave	VBN
cord-268139-tgpsu4qz	330	11	rapidly	rapidly	RB
cord-268139-tgpsu4qz	330	12	at	at	IN
cord-268139-tgpsu4qz	330	13	cleavage	cleavage	NN
cord-268139-tgpsu4qz	330	14	site	site	NN
cord-268139-tgpsu4qz	330	15	1	1	CD
cord-268139-tgpsu4qz	330	16	(	(	-LRB-
cord-268139-tgpsu4qz	330	17	CS1	CS1	NNP
cord-268139-tgpsu4qz	330	18	)	)	-RRB-
cord-268139-tgpsu4qz	330	19	as	as	IN
cord-268139-tgpsu4qz	330	20	the	the	DT
cord-268139-tgpsu4qz	330	21	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	330	22	is	be	VBZ
cord-268139-tgpsu4qz	330	23	translated	translate	VBN
cord-268139-tgpsu4qz	330	24	(	(	-LRB-
cord-268139-tgpsu4qz	330	25	indicated	indicate	VBN
cord-268139-tgpsu4qz	330	26	by	by	IN
cord-268139-tgpsu4qz	330	27	forward	forward	JJ
cord-268139-tgpsu4qz	330	28	slashes	slash	NNS
cord-268139-tgpsu4qz	330	29	)	)	-RRB-
cord-268139-tgpsu4qz	330	30	.	.	.
cord-268139-tgpsu4qz	331	1	Next	next	RB
cord-268139-tgpsu4qz	331	2	,	,	,
cord-268139-tgpsu4qz	331	3	cleavage	cleavage	NN
cord-268139-tgpsu4qz	331	4	site	site	NN
cord-268139-tgpsu4qz	331	5	3	3	CD
cord-268139-tgpsu4qz	331	6	(	(	-LRB-
cord-268139-tgpsu4qz	331	7	CS3	CS3	NNP
cord-268139-tgpsu4qz	331	8	)	)	-RRB-
cord-268139-tgpsu4qz	331	9	is	be	VBZ
cord-268139-tgpsu4qz	331	10	processed	process	VBN
cord-268139-tgpsu4qz	331	11	to	to	TO
cord-268139-tgpsu4qz	331	12	yield	yield	VB
cord-268139-tgpsu4qz	331	13	a	a	DT
cord-268139-tgpsu4qz	331	14	275-kDa	275-kda	CD
cord-268139-tgpsu4qz	331	15	nsp2	nsp2	NN
cord-268139-tgpsu4qz	331	16	-nsp3	-nsp3	NN
cord-268139-tgpsu4qz	331	17	precursor	precursor	NN
cord-268139-tgpsu4qz	331	18	.	.	.
cord-268139-tgpsu4qz	332	1	Finally	finally	RB
cord-268139-tgpsu4qz	332	2	,	,	,
cord-268139-tgpsu4qz	332	3	cleavage	cleavage	NN
cord-268139-tgpsu4qz	332	4	site	site	NN
cord-268139-tgpsu4qz	332	5	2	2	CD
cord-268139-tgpsu4qz	332	6	(	(	-LRB-
cord-268139-tgpsu4qz	332	7	CS2	CS2	NNP
cord-268139-tgpsu4qz	332	8	)	)	-RRB-
cord-268139-tgpsu4qz	332	9	is	be	VBZ
cord-268139-tgpsu4qz	332	10	cleaved	cleave	VBN
cord-268139-tgpsu4qz	332	11	to	to	TO
cord-268139-tgpsu4qz	332	12	liberate	liberate	VB
cord-268139-tgpsu4qz	332	13	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	332	14	(	(	-LRB-
cord-268139-tgpsu4qz	332	15	65	65	CD
cord-268139-tgpsu4qz	332	16	kDa	kDa	NNPS
cord-268139-tgpsu4qz	332	17	)	)	-RRB-
cord-268139-tgpsu4qz	332	18	and	and	CC
cord-268139-tgpsu4qz	332	19	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	332	20	(	(	-LRB-
cord-268139-tgpsu4qz	332	21	210	210	CD
cord-268139-tgpsu4qz	332	22	kDa	kDa	NNPS
cord-268139-tgpsu4qz	332	23	)	)	-RRB-
cord-268139-tgpsu4qz	332	24	.	.	.
cord-268139-tgpsu4qz	333	1	(	(	-LRB-
cord-268139-tgpsu4qz	333	2	B	b	NN
cord-268139-tgpsu4qz	333	3	)	)	-RRB-
cord-268139-tgpsu4qz	334	1	Wildtype	Wildtype	NNP
cord-268139-tgpsu4qz	334	2	pattern	pattern	NN
cord-268139-tgpsu4qz	334	3	of	of	IN
cord-268139-tgpsu4qz	334	4	processing	processing	NN
cord-268139-tgpsu4qz	334	5	for	for	IN
cord-268139-tgpsu4qz	334	6	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	334	7	mutant	mutant	NN
cord-268139-tgpsu4qz	334	8	.	.	.
cord-268139-tgpsu4qz	335	1	The	the	DT
cord-268139-tgpsu4qz	335	2	order	order	NN
cord-268139-tgpsu4qz	335	3	of	of	IN
cord-268139-tgpsu4qz	335	4	cleavage	cleavage	NN
cord-268139-tgpsu4qz	335	5	for	for	IN
cord-268139-tgpsu4qz	335	6	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	335	7	mutant	mutant	NNP
cord-268139-tgpsu4qz	335	8	polyprotein	polyprotein	NNP
cord-268139-tgpsu4qz	335	9	is	be	VBZ
cord-268139-tgpsu4qz	335	10	identical	identical	JJ
cord-268139-tgpsu4qz	335	11	to	to	IN
cord-268139-tgpsu4qz	335	12	that	that	DT
cord-268139-tgpsu4qz	335	13	of	of	IN
cord-268139-tgpsu4qz	335	14	icwt	icwt	NNP
cord-268139-tgpsu4qz	335	15	.	.	.
cord-268139-tgpsu4qz	336	1	Nsp1DC	Nsp1DC	NNP
cord-268139-tgpsu4qz	336	2	protein	protein	NN
cord-268139-tgpsu4qz	336	3	(	(	-LRB-
cord-268139-tgpsu4qz	336	4	DC	DC	NNP
cord-268139-tgpsu4qz	336	5	;	;	:
cord-268139-tgpsu4qz	336	6	14	14	CD
cord-268139-tgpsu4qz	336	7	kDa	kDa	NNS
cord-268139-tgpsu4qz	336	8	)	)	-RRB-
cord-268139-tgpsu4qz	336	9	is	be	VBZ
cord-268139-tgpsu4qz	336	10	liberated	liberate	VBN
cord-268139-tgpsu4qz	336	11	by	by	IN
cord-268139-tgpsu4qz	336	12	CS1	CS1	NNP
cord-268139-tgpsu4qz	336	13	cleavage	cleavage	NN
cord-268139-tgpsu4qz	336	14	.	.	.
cord-268139-tgpsu4qz	337	1	Next	next	RB
cord-268139-tgpsu4qz	337	2	,	,	,
cord-268139-tgpsu4qz	337	3	CS3	CS3	NNP
cord-268139-tgpsu4qz	337	4	is	be	VBZ
cord-268139-tgpsu4qz	337	5	processed	process	VBN
cord-268139-tgpsu4qz	337	6	to	to	TO
cord-268139-tgpsu4qz	337	7	yield	yield	VB
cord-268139-tgpsu4qz	337	8	a	a	DT
cord-268139-tgpsu4qz	337	9	275-kDa	275-kda	CD
cord-268139-tgpsu4qz	337	10	nsp2	nsp2	NN
cord-268139-tgpsu4qz	337	11	-nsp3	-nsp3	NN
cord-268139-tgpsu4qz	337	12	precursor	precursor	NN
cord-268139-tgpsu4qz	337	13	.	.	.
cord-268139-tgpsu4qz	338	1	Finally	finally	RB
cord-268139-tgpsu4qz	338	2	,	,	,
cord-268139-tgpsu4qz	338	3	CS2	CS2	NNP
cord-268139-tgpsu4qz	338	4	is	be	VBZ
cord-268139-tgpsu4qz	338	5	cleaved	cleave	VBN
cord-268139-tgpsu4qz	338	6	to	to	TO
cord-268139-tgpsu4qz	338	7	liberate	liberate	VB
cord-268139-tgpsu4qz	338	8	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	338	9	(	(	-LRB-
cord-268139-tgpsu4qz	338	10	65	65	CD
cord-268139-tgpsu4qz	338	11	kDa	kDa	NNPS
cord-268139-tgpsu4qz	338	12	)	)	-RRB-
cord-268139-tgpsu4qz	338	13	and	and	CC
cord-268139-tgpsu4qz	338	14	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	338	15	(	(	-LRB-
cord-268139-tgpsu4qz	338	16	210	210	CD
cord-268139-tgpsu4qz	338	17	kDa	kDa	NNPS
cord-268139-tgpsu4qz	338	18	)	)	-RRB-
cord-268139-tgpsu4qz	338	19	.	.	.
cord-268139-tgpsu4qz	339	1	(	(	-LRB-
cord-268139-tgpsu4qz	339	2	C	C	NNP
cord-268139-tgpsu4qz	339	3	)	)	-RRB-
cord-268139-tgpsu4qz	339	4	Alternative	alternative	JJ
cord-268139-tgpsu4qz	339	5	pattern	pattern	NN
cord-268139-tgpsu4qz	339	6	of	of	IN
cord-268139-tgpsu4qz	339	7	processing	processing	NN
cord-268139-tgpsu4qz	339	8	for	for	IN
cord-268139-tgpsu4qz	339	9	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	339	10	mutant	mutant	NN
cord-268139-tgpsu4qz	339	11	.	.	.
cord-268139-tgpsu4qz	340	1	If	if	IN
cord-268139-tgpsu4qz	340	2	CS1	CS1	NNP
cord-268139-tgpsu4qz	340	3	is	be	VBZ
cord-268139-tgpsu4qz	340	4	not	not	RB
cord-268139-tgpsu4qz	340	5	initially	initially	RB
cord-268139-tgpsu4qz	340	6	cleaved	cleave	VBN
cord-268139-tgpsu4qz	340	7	,	,	,
cord-268139-tgpsu4qz	340	8	a	a	DT
cord-268139-tgpsu4qz	340	9	290-kDa	290-kda	CD
cord-268139-tgpsu4qz	340	10	nsp1DC	nsp1dc	NN
cord-268139-tgpsu4qz	340	11	-nsp2	-nsp2	.
cord-268139-tgpsu4qz	341	1	-nsp3	-nsp3	NNPS
cord-268139-tgpsu4qz	342	1	precursor	precursor	NN
cord-268139-tgpsu4qz	342	2	is	be	VBZ
cord-268139-tgpsu4qz	342	3	made	make	VBN
cord-268139-tgpsu4qz	342	4	following	follow	VBG
cord-268139-tgpsu4qz	342	5	CS3	CS3	NNP
cord-268139-tgpsu4qz	342	6	processing	processing	NN
cord-268139-tgpsu4qz	342	7	.	.	.
cord-268139-tgpsu4qz	343	1	This	this	DT
cord-268139-tgpsu4qz	343	2	precursor	precursor	NN
cord-268139-tgpsu4qz	343	3	is	be	VBZ
cord-268139-tgpsu4qz	343	4	then	then	RB
cord-268139-tgpsu4qz	343	5	cleaved	cleave	VBN
cord-268139-tgpsu4qz	343	6	at	at	IN
cord-268139-tgpsu4qz	343	7	CS2	CS2	NNP
cord-268139-tgpsu4qz	343	8	to	to	TO
cord-268139-tgpsu4qz	343	9	yield	yield	VB
cord-268139-tgpsu4qz	343	10	an	an	DT
cord-268139-tgpsu4qz	343	11	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	343	12	protein	protein	NN
cord-268139-tgpsu4qz	343	13	(	(	-LRB-
cord-268139-tgpsu4qz	343	14	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	343	15	-nsp2	-nsp2	NNP
cord-268139-tgpsu4qz	343	16	)	)	-RRB-
cord-268139-tgpsu4qz	343	17	and	and	CC
cord-268139-tgpsu4qz	343	18	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	343	19	.	.	.
cord-268139-tgpsu4qz	344	1	The	the	DT
cord-268139-tgpsu4qz	344	2	results	result	NNS
cord-268139-tgpsu4qz	344	3	described	describe	VBN
cord-268139-tgpsu4qz	344	4	in	in	IN
cord-268139-tgpsu4qz	344	5	this	this	DT
cord-268139-tgpsu4qz	344	6	report	report	NN
cord-268139-tgpsu4qz	344	7	are	be	VBP
cord-268139-tgpsu4qz	344	8	consistent	consistent	JJ
cord-268139-tgpsu4qz	344	9	with	with	IN
cord-268139-tgpsu4qz	344	10	a	a	DT
cord-268139-tgpsu4qz	344	11	model	model	NN
cord-268139-tgpsu4qz	344	12	in	in	IN
cord-268139-tgpsu4qz	344	13	which	which	WDT
cord-268139-tgpsu4qz	344	14	the	the	DT
cord-268139-tgpsu4qz	344	15	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	344	16	gene	gene	NN
cord-268139-tgpsu4qz	344	17	1	1	CD
cord-268139-tgpsu4qz	344	18	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	344	19	exists	exist	VBZ
cord-268139-tgpsu4qz	344	20	in	in	IN
cord-268139-tgpsu4qz	344	21	two	two	CD
cord-268139-tgpsu4qz	344	22	different	different	JJ
cord-268139-tgpsu4qz	344	23	conformations	conformation	NNS
cord-268139-tgpsu4qz	344	24	:	:	:
cord-268139-tgpsu4qz	344	25	a	a	DT
cord-268139-tgpsu4qz	344	26	CS1	CS1	NNP
cord-268139-tgpsu4qz	344	27	cleavable	cleavable	JJ
cord-268139-tgpsu4qz	344	28	form	form	NN
cord-268139-tgpsu4qz	344	29	and	and	CC
cord-268139-tgpsu4qz	344	30	a	a	DT
cord-268139-tgpsu4qz	344	31	CS1	CS1	NNP
cord-268139-tgpsu4qz	344	32	non	non	JJ
cord-268139-tgpsu4qz	344	33	-	-	JJ
cord-268139-tgpsu4qz	344	34	cleavable	cleavable	JJ
cord-268139-tgpsu4qz	344	35	from	from	IN
cord-268139-tgpsu4qz	344	36	.	.	.
cord-268139-tgpsu4qz	345	1	If	if	IN
cord-268139-tgpsu4qz	345	2	the	the	DT
cord-268139-tgpsu4qz	345	3	mutant	mutant	JJ
cord-268139-tgpsu4qz	345	4	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	345	5	is	be	VBZ
cord-268139-tgpsu4qz	345	6	folded	fold	VBN
cord-268139-tgpsu4qz	345	7	in	in	IN
cord-268139-tgpsu4qz	345	8	such	such	PDT
cord-268139-tgpsu4qz	345	9	a	a	DT
cord-268139-tgpsu4qz	345	10	manner	manner	NN
cord-268139-tgpsu4qz	345	11	that	that	WDT
cord-268139-tgpsu4qz	345	12	CS1	CS1	NNP
cord-268139-tgpsu4qz	345	13	is	be	VBZ
cord-268139-tgpsu4qz	345	14	accessible	accessible	JJ
cord-268139-tgpsu4qz	345	15	by	by	IN
cord-268139-tgpsu4qz	345	16	the	the	DT
cord-268139-tgpsu4qz	345	17	proteinase	proteinase	NN
cord-268139-tgpsu4qz	345	18	,	,	,
cord-268139-tgpsu4qz	345	19	then	then	RB
cord-268139-tgpsu4qz	345	20	this	this	DT
cord-268139-tgpsu4qz	345	21	site	site	NN
cord-268139-tgpsu4qz	345	22	is	be	VBZ
cord-268139-tgpsu4qz	345	23	cleaved	cleave	VBN
cord-268139-tgpsu4qz	345	24	normally	normally	RB
cord-268139-tgpsu4qz	345	25	and	and	CC
cord-268139-tgpsu4qz	345	26	the	the	DT
cord-268139-tgpsu4qz	345	27	mutant	mutant	JJ
cord-268139-tgpsu4qz	345	28	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	345	29	follows	follow	VBZ
cord-268139-tgpsu4qz	345	30	a	a	DT
cord-268139-tgpsu4qz	345	31	wild	wild	JJ
cord-268139-tgpsu4qz	345	32	-	-	HYPH
cord-268139-tgpsu4qz	345	33	type	type	NN
cord-268139-tgpsu4qz	345	34	pattern	pattern	NN
cord-268139-tgpsu4qz	345	35	of	of	IN
cord-268139-tgpsu4qz	345	36	processing	processing	NN
cord-268139-tgpsu4qz	345	37	(	(	-LRB-
cord-268139-tgpsu4qz	345	38	Fig	Fig	NNP
cord-268139-tgpsu4qz	345	39	.	.	.
cord-268139-tgpsu4qz	345	40	7B	7B	NNP
cord-268139-tgpsu4qz	345	41	)	)	-RRB-
cord-268139-tgpsu4qz	345	42	.	.	.
cord-268139-tgpsu4qz	346	1	Alternatively	alternatively	RB
cord-268139-tgpsu4qz	346	2	,	,	,
cord-268139-tgpsu4qz	346	3	if	if	IN
cord-268139-tgpsu4qz	346	4	the	the	DT
cord-268139-tgpsu4qz	346	5	mutant	mutant	JJ
cord-268139-tgpsu4qz	346	6	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	346	7	is	be	VBZ
cord-268139-tgpsu4qz	346	8	folded	fold	VBN
cord-268139-tgpsu4qz	346	9	in	in	IN
cord-268139-tgpsu4qz	346	10	a	a	DT
cord-268139-tgpsu4qz	346	11	way	way	NN
cord-268139-tgpsu4qz	346	12	that	that	WDT
cord-268139-tgpsu4qz	346	13	masks	mask	VBZ
cord-268139-tgpsu4qz	346	14	CS1	CS1	NNP
cord-268139-tgpsu4qz	346	15	,	,	,
cord-268139-tgpsu4qz	346	16	this	this	DT
cord-268139-tgpsu4qz	346	17	site	site	NN
cord-268139-tgpsu4qz	346	18	is	be	VBZ
cord-268139-tgpsu4qz	346	19	not	not	RB
cord-268139-tgpsu4qz	346	20	processed	process	VBN
cord-268139-tgpsu4qz	346	21	and	and	CC
cord-268139-tgpsu4qz	346	22	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	346	23	remains	remain	VBZ
cord-268139-tgpsu4qz	346	24	fused	fuse	VBN
cord-268139-tgpsu4qz	346	25	to	to	IN
cord-268139-tgpsu4qz	346	26	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	346	27	(	(	-LRB-
cord-268139-tgpsu4qz	346	28	Fig	Fig	NNP
cord-268139-tgpsu4qz	346	29	.	.	.
cord-268139-tgpsu4qz	346	30	7C	7C	NNP
cord-268139-tgpsu4qz	346	31	)	)	-RRB-
cord-268139-tgpsu4qz	346	32	.	.	.
cord-268139-tgpsu4qz	347	1	In	in	IN
cord-268139-tgpsu4qz	347	2	this	this	DT
cord-268139-tgpsu4qz	347	3	model	model	NN
cord-268139-tgpsu4qz	347	4	,	,	,
cord-268139-tgpsu4qz	347	5	an	an	DT
cord-268139-tgpsu4qz	347	6	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	347	7	-	-	HYPH
cord-268139-tgpsu4qz	347	8	nsp2	nsp2	NN
cord-268139-tgpsu4qz	347	9	-nsp3	-nsp3	NNP
cord-268139-tgpsu4qz	347	10	precursor	precursor	NN
cord-268139-tgpsu4qz	347	11	is	be	VBZ
cord-268139-tgpsu4qz	347	12	the	the	DT
cord-268139-tgpsu4qz	347	13	first	first	RB
cord-268139-tgpsu4qz	347	14	processed	process	VBN
cord-268139-tgpsu4qz	347	15	protein	protein	NN
cord-268139-tgpsu4qz	347	16	following	follow	VBG
cord-268139-tgpsu4qz	347	17	CS3	CS3	NNP
cord-268139-tgpsu4qz	347	18	cleavage	cleavage	NN
cord-268139-tgpsu4qz	347	19	.	.	.
cord-268139-tgpsu4qz	348	1	This	this	DT
cord-268139-tgpsu4qz	348	2	polypeptide	polypeptide	NN
cord-268139-tgpsu4qz	348	3	is	be	VBZ
cord-268139-tgpsu4qz	348	4	then	then	RB
cord-268139-tgpsu4qz	348	5	processed	process	VBN
cord-268139-tgpsu4qz	348	6	at	at	IN
cord-268139-tgpsu4qz	348	7	CS2	CS2	NNP
cord-268139-tgpsu4qz	348	8	to	to	TO
cord-268139-tgpsu4qz	348	9	yield	yield	VB
cord-268139-tgpsu4qz	348	10	the	the	DT
cord-268139-tgpsu4qz	348	11	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	348	12	protein	protein	NN
cord-268139-tgpsu4qz	348	13	(	(	-LRB-
cord-268139-tgpsu4qz	348	14	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	348	15	-nsp2	-nsp2	NNP
cord-268139-tgpsu4qz	348	16	)	)	-RRB-
cord-268139-tgpsu4qz	348	17	and	and	CC
cord-268139-tgpsu4qz	348	18	nsp3	nsp3	NNS
cord-268139-tgpsu4qz	348	19	.	.	.
cord-268139-tgpsu4qz	349	1	Although	although	IN
cord-268139-tgpsu4qz	349	2	the	the	DT
cord-268139-tgpsu4qz	349	3	high	high	JJ
cord-268139-tgpsu4qz	349	4	molecular	molecular	JJ
cord-268139-tgpsu4qz	349	5	weight	weight	NN
cord-268139-tgpsu4qz	349	6	precursors	precursor	NNS
cord-268139-tgpsu4qz	349	7	were	be	VBD
cord-268139-tgpsu4qz	349	8	not	not	RB
cord-268139-tgpsu4qz	349	9	detected	detect	VBN
cord-268139-tgpsu4qz	349	10	in	in	IN
cord-268139-tgpsu4qz	349	11	these	these	DT
cord-268139-tgpsu4qz	349	12	studies	study	NNS
cord-268139-tgpsu4qz	349	13	,	,	,
cord-268139-tgpsu4qz	349	14	the	the	DT
cord-268139-tgpsu4qz	349	15	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	349	16	of	of	IN
cord-268139-tgpsu4qz	349	17	appearance	appearance	NN
cord-268139-tgpsu4qz	349	18	for	for	IN
cord-268139-tgpsu4qz	349	19	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	349	20	,	,	,
cord-268139-tgpsu4qz	349	21	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	349	22	,	,	,
cord-268139-tgpsu4qz	349	23	and	and	CC
cord-268139-tgpsu4qz	349	24	the	the	DT
cord-268139-tgpsu4qz	349	25	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	349	26	protein	protein	NN
cord-268139-tgpsu4qz	349	27	are	be	VBP
cord-268139-tgpsu4qz	349	28	compatible	compatible	JJ
cord-268139-tgpsu4qz	349	29	with	with	IN
cord-268139-tgpsu4qz	349	30	the	the	DT
cord-268139-tgpsu4qz	349	31	proposed	propose	VBN
cord-268139-tgpsu4qz	349	32	model	model	NN
cord-268139-tgpsu4qz	349	33	.	.	.
cord-268139-tgpsu4qz	350	1	During	during	IN
cord-268139-tgpsu4qz	350	2	pulse	pulse	NN
cord-268139-tgpsu4qz	350	3	-	-	HYPH
cord-268139-tgpsu4qz	350	4	chase	chase	NN
cord-268139-tgpsu4qz	350	5	experiments	experiment	NNS
cord-268139-tgpsu4qz	350	6	,	,	,
cord-268139-tgpsu4qz	350	7	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	350	8	and	and	CC
cord-268139-tgpsu4qz	350	9	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	350	10	are	be	VBP
cord-268139-tgpsu4qz	350	11	first	first	RB
cord-268139-tgpsu4qz	350	12	detected	detect	VBN
cord-268139-tgpsu4qz	350	13	in	in	IN
cord-268139-tgpsu4qz	350	14	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	350	15	virus	virus	NN
cord-268139-tgpsu4qz	350	16	-	-	HYPH
cord-268139-tgpsu4qz	350	17	infected	infect	VBN
cord-268139-tgpsu4qz	350	18	cells	cell	NNS
cord-268139-tgpsu4qz	350	19	with	with	IN
cord-268139-tgpsu4qz	350	20	the	the	DT
cord-268139-tgpsu4qz	350	21	same	same	JJ
cord-268139-tgpsu4qz	350	22	timing	timing	NN
cord-268139-tgpsu4qz	350	23	as	as	IN
cord-268139-tgpsu4qz	351	1	the	the	DT
cord-268139-tgpsu4qz	351	2	corresponding	correspond	VBG
cord-268139-tgpsu4qz	351	3	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	351	4	and	and	CC
cord-268139-tgpsu4qz	351	5	nsp2	nsp2	NN
cord-268139-tgpsu4qz	351	6	proteins	protein	NNS
cord-268139-tgpsu4qz	351	7	in	in	IN
cord-268139-tgpsu4qz	351	8	icwt	icwt	NNP
cord-268139-tgpsu4qz	351	9	-	-	HYPH
cord-268139-tgpsu4qz	351	10	infected	infect	VBN
cord-268139-tgpsu4qz	351	11	cells	cell	NNS
cord-268139-tgpsu4qz	351	12	.	.	.
cord-268139-tgpsu4qz	352	1	However	however	RB
cord-268139-tgpsu4qz	352	2	,	,	,
cord-268139-tgpsu4qz	352	3	the	the	DT
cord-268139-tgpsu4qz	352	4	expression	expression	NN
cord-268139-tgpsu4qz	352	5	kinetics	kinetic	NNS
cord-268139-tgpsu4qz	352	6	for	for	IN
cord-268139-tgpsu4qz	352	7	the	the	DT
cord-268139-tgpsu4qz	352	8	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	353	1	protein	protein	NN
cord-268139-tgpsu4qz	353	2	are	be	VBP
cord-268139-tgpsu4qz	353	3	unlike	unlike	IN
cord-268139-tgpsu4qz	353	4	either	either	CC
cord-268139-tgpsu4qz	353	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	353	6	or	or	CC
cord-268139-tgpsu4qz	353	7	nsp2	nsp2	NN
cord-268139-tgpsu4qz	353	8	,	,	,
cord-268139-tgpsu4qz	353	9	suggesting	suggest	VBG
cord-268139-tgpsu4qz	353	10	that	that	IN
cord-268139-tgpsu4qz	353	11	the	the	DT
cord-268139-tgpsu4qz	353	12	timing	timing	NN
cord-268139-tgpsu4qz	353	13	and	and	CC
cord-268139-tgpsu4qz	353	14	order	order	NN
cord-268139-tgpsu4qz	353	15	of	of	IN
cord-268139-tgpsu4qz	353	16	polyprotein	polyprotein	JJ
cord-268139-tgpsu4qz	353	17	processing	processing	NN
cord-268139-tgpsu4qz	353	18	are	be	VBP
cord-268139-tgpsu4qz	353	19	different	different	JJ
cord-268139-tgpsu4qz	353	20	under	under	IN
cord-268139-tgpsu4qz	353	21	circumstances	circumstance	NNS
cord-268139-tgpsu4qz	353	22	where	where	WRB
cord-268139-tgpsu4qz	353	23	CS1	CS1	NNP
cord-268139-tgpsu4qz	353	24	is	be	VBZ
cord-268139-tgpsu4qz	353	25	not	not	RB
cord-268139-tgpsu4qz	353	26	immediately	immediately	RB
cord-268139-tgpsu4qz	353	27	cleaved	cleave	VBN
cord-268139-tgpsu4qz	353	28	.	.	.
cord-268139-tgpsu4qz	354	1	Whether	whether	IN
cord-268139-tgpsu4qz	354	2	the	the	DT
cord-268139-tgpsu4qz	354	3	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	354	4	protein	protein	NN
cord-268139-tgpsu4qz	354	5	is	be	VBZ
cord-268139-tgpsu4qz	354	6	capable	capable	JJ
cord-268139-tgpsu4qz	354	7	of	of	IN
cord-268139-tgpsu4qz	354	8	being	be	VBG
cord-268139-tgpsu4qz	354	9	subsequently	subsequently	RB
cord-268139-tgpsu4qz	354	10	processed	process	VBN
cord-268139-tgpsu4qz	354	11	into	into	IN
cord-268139-tgpsu4qz	354	12	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	354	13	and	and	CC
cord-268139-tgpsu4qz	354	14	nsp2	nsp2	NN
cord-268139-tgpsu4qz	354	15	could	could	MD
cord-268139-tgpsu4qz	354	16	not	not	RB
cord-268139-tgpsu4qz	354	17	be	be	VB
cord-268139-tgpsu4qz	354	18	determined	determine	VBN
cord-268139-tgpsu4qz	354	19	from	from	IN
cord-268139-tgpsu4qz	354	20	these	these	DT
cord-268139-tgpsu4qz	354	21	studies	study	NNS
cord-268139-tgpsu4qz	354	22	.	.	.
cord-268139-tgpsu4qz	355	1	At	at	IN
cord-268139-tgpsu4qz	355	2	extended	extended	JJ
cord-268139-tgpsu4qz	355	3	times	time	NNS
cord-268139-tgpsu4qz	355	4	p.c	p.c	NNP
cord-268139-tgpsu4qz	355	5	.	.	NNP
cord-268139-tgpsu4qz	355	6	(	(	-LRB-
cord-268139-tgpsu4qz	355	7	360	360	CD
cord-268139-tgpsu4qz	355	8	min	min	NNP
cord-268139-tgpsu4qz	355	9	)	)	-RRB-
cord-268139-tgpsu4qz	355	10	,	,	,
cord-268139-tgpsu4qz	355	11	the	the	DT
cord-268139-tgpsu4qz	355	12	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	355	13	protein	protein	NN
cord-268139-tgpsu4qz	355	14	is	be	VBZ
cord-268139-tgpsu4qz	355	15	still	still	RB
cord-268139-tgpsu4qz	355	16	detectable	detectable	JJ
cord-268139-tgpsu4qz	355	17	,	,	,
cord-268139-tgpsu4qz	355	18	albeit	albeit	IN
cord-268139-tgpsu4qz	355	19	at	at	IN
cord-268139-tgpsu4qz	355	20	slightly	slightly	RB
cord-268139-tgpsu4qz	355	21	reduced	reduce	VBN
cord-268139-tgpsu4qz	355	22	levels	level	NNS
cord-268139-tgpsu4qz	355	23	,	,	,
cord-268139-tgpsu4qz	355	24	and	and	CC
cord-268139-tgpsu4qz	355	25	there	there	EX
cord-268139-tgpsu4qz	355	26	is	be	VBZ
cord-268139-tgpsu4qz	355	27	no	no	DT
cord-268139-tgpsu4qz	355	28	new	new	JJ
cord-268139-tgpsu4qz	355	29	accumulation	accumulation	NN
cord-268139-tgpsu4qz	355	30	of	of	IN
cord-268139-tgpsu4qz	355	31	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	355	32	at	at	IN
cord-268139-tgpsu4qz	355	33	this	this	DT
cord-268139-tgpsu4qz	355	34	same	same	JJ
cord-268139-tgpsu4qz	355	35	time	time	NN
cord-268139-tgpsu4qz	355	36	.	.	.
cord-268139-tgpsu4qz	356	1	These	these	DT
cord-268139-tgpsu4qz	356	2	data	datum	NNS
cord-268139-tgpsu4qz	356	3	are	be	VBP
cord-268139-tgpsu4qz	356	4	most	most	RBS
cord-268139-tgpsu4qz	356	5	consistent	consistent	JJ
cord-268139-tgpsu4qz	356	6	with	with	IN
cord-268139-tgpsu4qz	356	7	the	the	DT
cord-268139-tgpsu4qz	356	8	idea	idea	NN
cord-268139-tgpsu4qz	356	9	that	that	IN
cord-268139-tgpsu4qz	356	10	the	the	DT
cord-268139-tgpsu4qz	356	11	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	356	12	protein	protein	NN
cord-268139-tgpsu4qz	356	13	is	be	VBZ
cord-268139-tgpsu4qz	356	14	not	not	RB
cord-268139-tgpsu4qz	356	15	further	further	RB
cord-268139-tgpsu4qz	356	16	processed	process	VBN
cord-268139-tgpsu4qz	356	17	once	once	IN
cord-268139-tgpsu4qz	356	18	it	-PRON-	PRP
cord-268139-tgpsu4qz	356	19	is	be	VBZ
cord-268139-tgpsu4qz	356	20	generated	generate	VBN
cord-268139-tgpsu4qz	356	21	.	.	.
cord-268139-tgpsu4qz	357	1	In	in	IN
cord-268139-tgpsu4qz	357	2	addition	addition	NN
cord-268139-tgpsu4qz	357	3	to	to	IN
cord-268139-tgpsu4qz	357	4	CS1	CS1	NNP
cord-268139-tgpsu4qz	357	5	processing	processing	NN
cord-268139-tgpsu4qz	357	6	defects	defect	NNS
cord-268139-tgpsu4qz	357	7	,	,	,
cord-268139-tgpsu4qz	357	8	the	the	DT
cord-268139-tgpsu4qz	357	9	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	357	10	virus	virus	NN
cord-268139-tgpsu4qz	357	11	also	also	RB
cord-268139-tgpsu4qz	357	12	exhibits	exhibit	VBZ
cord-268139-tgpsu4qz	357	13	subtle	subtle	JJ
cord-268139-tgpsu4qz	357	14	replication	replication	NN
cord-268139-tgpsu4qz	357	15	defects	defect	NNS
cord-268139-tgpsu4qz	357	16	,	,	,
cord-268139-tgpsu4qz	357	17	but	but	CC
cord-268139-tgpsu4qz	357	18	normal	normal	JJ
cord-268139-tgpsu4qz	357	19	viral	viral	JJ
cord-268139-tgpsu4qz	357	20	RNA	rna	NN
cord-268139-tgpsu4qz	357	21	synthesis	synthesis	NN
cord-268139-tgpsu4qz	357	22	levels	level	NNS
cord-268139-tgpsu4qz	357	23	,	,	,
cord-268139-tgpsu4qz	357	24	compared	compare	VBN
cord-268139-tgpsu4qz	357	25	with	with	IN
cord-268139-tgpsu4qz	357	26	icwt	icwt	NNP
cord-268139-tgpsu4qz	357	27	.	.	.
cord-268139-tgpsu4qz	358	1	A	a	DT
cord-268139-tgpsu4qz	358	2	unique	unique	JJ
cord-268139-tgpsu4qz	358	3	RNA	RNA	NNP
cord-268139-tgpsu4qz	358	4	species	specie	NNS
cord-268139-tgpsu4qz	358	5	migrating	migrate	VBG
cord-268139-tgpsu4qz	358	6	above	above	IN
cord-268139-tgpsu4qz	358	7	RNA4	RNA4	NNP
cord-268139-tgpsu4qz	358	8	was	be	VBD
cord-268139-tgpsu4qz	358	9	detected	detect	VBN
cord-268139-tgpsu4qz	358	10	in	in	IN
cord-268139-tgpsu4qz	358	11	nsp1DCinfected	nsp1DCinfected	NNP
cord-268139-tgpsu4qz	358	12	cells	cell	NNS
cord-268139-tgpsu4qz	358	13	.	.	.
cord-268139-tgpsu4qz	359	1	The	the	DT
cord-268139-tgpsu4qz	359	2	identity	identity	NN
cord-268139-tgpsu4qz	359	3	of	of	IN
cord-268139-tgpsu4qz	359	4	this	this	DT
cord-268139-tgpsu4qz	359	5	RNA	RNA	NNP
cord-268139-tgpsu4qz	359	6	band	band	NN
cord-268139-tgpsu4qz	359	7	remains	remain	VBZ
cord-268139-tgpsu4qz	359	8	to	to	TO
cord-268139-tgpsu4qz	359	9	be	be	VB
cord-268139-tgpsu4qz	359	10	determined	determine	VBN
cord-268139-tgpsu4qz	359	11	,	,	,
cord-268139-tgpsu4qz	359	12	but	but	CC
cord-268139-tgpsu4qz	359	13	it	-PRON-	PRP
cord-268139-tgpsu4qz	359	14	might	may	MD
cord-268139-tgpsu4qz	359	15	represent	represent	VB
cord-268139-tgpsu4qz	359	16	a	a	DT
cord-268139-tgpsu4qz	359	17	stable	stable	JJ
cord-268139-tgpsu4qz	359	18	replicative	replicative	JJ
cord-268139-tgpsu4qz	359	19	intermediate	intermediate	NN
cord-268139-tgpsu4qz	359	20	and	and	CC
cord-268139-tgpsu4qz	359	21	implies	imply	VBZ
cord-268139-tgpsu4qz	359	22	that	that	IN
cord-268139-tgpsu4qz	359	23	this	this	DT
cord-268139-tgpsu4qz	359	24	mutant	mutant	JJ
cord-268139-tgpsu4qz	359	25	virus	virus	NN
cord-268139-tgpsu4qz	359	26	may	may	MD
cord-268139-tgpsu4qz	359	27	have	have	VB
cord-268139-tgpsu4qz	359	28	minor	minor	JJ
cord-268139-tgpsu4qz	359	29	defects	defect	NNS
cord-268139-tgpsu4qz	359	30	in	in	IN
cord-268139-tgpsu4qz	359	31	discontinuous	discontinuous	JJ
cord-268139-tgpsu4qz	359	32	transcription	transcription	NN
cord-268139-tgpsu4qz	359	33	.	.	.
cord-268139-tgpsu4qz	360	1	Another	another	DT
cord-268139-tgpsu4qz	360	2	interesting	interesting	JJ
cord-268139-tgpsu4qz	360	3	possibility	possibility	NN
cord-268139-tgpsu4qz	360	4	is	be	VBZ
cord-268139-tgpsu4qz	360	5	that	that	IN
cord-268139-tgpsu4qz	360	6	this	this	DT
cord-268139-tgpsu4qz	360	7	band	band	NN
cord-268139-tgpsu4qz	360	8	might	may	MD
cord-268139-tgpsu4qz	360	9	represent	represent	VB
cord-268139-tgpsu4qz	360	10	a	a	DT
cord-268139-tgpsu4qz	360	11	new	new	JJ
cord-268139-tgpsu4qz	360	12	species	specie	NNS
cord-268139-tgpsu4qz	360	13	of	of	IN
cord-268139-tgpsu4qz	360	14	subgenomic	subgenomic	NNP
cord-268139-tgpsu4qz	360	15	RNA	RNA	NNP
cord-268139-tgpsu4qz	360	16	.	.	.
cord-268139-tgpsu4qz	361	1	Because	because	IN
cord-268139-tgpsu4qz	361	2	some	some	DT
cord-268139-tgpsu4qz	361	3	molecules	molecule	NNS
cord-268139-tgpsu4qz	361	4	of	of	IN
cord-268139-tgpsu4qz	361	5	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	361	6	are	be	VBP
cord-268139-tgpsu4qz	361	7	fused	fuse	VBN
cord-268139-tgpsu4qz	361	8	to	to	IN
cord-268139-tgpsu4qz	361	9	nsp2	nsp2	NN
cord-268139-tgpsu4qz	361	10	,	,	,
cord-268139-tgpsu4qz	361	11	defects	defect	NNS
cord-268139-tgpsu4qz	361	12	in	in	IN
cord-268139-tgpsu4qz	361	13	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	361	14	function	function	NN
cord-268139-tgpsu4qz	361	15	may	may	MD
cord-268139-tgpsu4qz	361	16	contribute	contribute	VB
cord-268139-tgpsu4qz	361	17	to	to	IN
cord-268139-tgpsu4qz	361	18	the	the	DT
cord-268139-tgpsu4qz	361	19	mutant	mutant	JJ
cord-268139-tgpsu4qz	361	20	viral	viral	JJ
cord-268139-tgpsu4qz	361	21	phenotype	phenotype	NN
cord-268139-tgpsu4qz	361	22	.	.	.
cord-268139-tgpsu4qz	362	1	Alternatively	alternatively	RB
cord-268139-tgpsu4qz	362	2	,	,	,
cord-268139-tgpsu4qz	362	3	the	the	DT
cord-268139-tgpsu4qz	362	4	reduced	reduce	VBN
cord-268139-tgpsu4qz	362	5	replication	replication	NN
cord-268139-tgpsu4qz	362	6	of	of	IN
cord-268139-tgpsu4qz	362	7	the	the	DT
cord-268139-tgpsu4qz	362	8	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	362	9	virus	virus	NN
cord-268139-tgpsu4qz	362	10	might	may	MD
cord-268139-tgpsu4qz	362	11	suggest	suggest	VB
cord-268139-tgpsu4qz	362	12	that	that	IN
cord-268139-tgpsu4qz	362	13	nsp1	nsp1	PRP
cord-268139-tgpsu4qz	362	14	plays	play	VBZ
cord-268139-tgpsu4qz	362	15	an	an	DT
cord-268139-tgpsu4qz	362	16	important	important	JJ
cord-268139-tgpsu4qz	362	17	role	role	NN
cord-268139-tgpsu4qz	362	18	downstream	downstream	RB
cord-268139-tgpsu4qz	362	19	of	of	IN
cord-268139-tgpsu4qz	362	20	viral	viral	JJ
cord-268139-tgpsu4qz	362	21	RNA	RNA	NNP
cord-268139-tgpsu4qz	362	22	synthesis	synthesis	NN
cord-268139-tgpsu4qz	362	23	,	,	,
cord-268139-tgpsu4qz	362	24	such	such	JJ
cord-268139-tgpsu4qz	362	25	as	as	IN
cord-268139-tgpsu4qz	362	26	in	in	IN
cord-268139-tgpsu4qz	362	27	virion	virion	NN
cord-268139-tgpsu4qz	362	28	assembly	assembly	NN
cord-268139-tgpsu4qz	362	29	.	.	.
cord-268139-tgpsu4qz	363	1	At	at	IN
cord-268139-tgpsu4qz	363	2	late	late	JJ
cord-268139-tgpsu4qz	363	3	times	time	NNS
cord-268139-tgpsu4qz	363	4	of	of	IN
cord-268139-tgpsu4qz	363	5	infection	infection	NN
cord-268139-tgpsu4qz	363	6	,	,	,
cord-268139-tgpsu4qz	363	7	wild	wild	JJ
cord-268139-tgpsu4qz	363	8	-	-	HYPH
cord-268139-tgpsu4qz	363	9	type	type	NN
cord-268139-tgpsu4qz	363	10	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	363	11	co	co	NN
cord-268139-tgpsu4qz	363	12	-	-	NN
cord-268139-tgpsu4qz	363	13	localizes	localize	VBZ
cord-268139-tgpsu4qz	363	14	with	with	IN
cord-268139-tgpsu4qz	363	15	the	the	DT
cord-268139-tgpsu4qz	363	16	MHV	MHV	NNP
cord-268139-tgpsu4qz	363	17	structural	structural	JJ
cord-268139-tgpsu4qz	363	18	protein	protein	NN
cord-268139-tgpsu4qz	363	19	M	M	NNP
cord-268139-tgpsu4qz	363	20	at	at	IN
cord-268139-tgpsu4qz	363	21	virion	virion	NN
cord-268139-tgpsu4qz	363	22	assembly	assembly	NN
cord-268139-tgpsu4qz	363	23	sites	site	NNS
cord-268139-tgpsu4qz	363	24	.	.	.
cord-268139-tgpsu4qz	364	1	Because	because	IN
cord-268139-tgpsu4qz	364	2	the	the	DT
cord-268139-tgpsu4qz	364	3	a	a	DT
cord-268139-tgpsu4qz	364	4	-	-	HYPH
cord-268139-tgpsu4qz	364	5	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	364	6	antisera	antisera	NN
cord-268139-tgpsu4qz	364	7	used	use	VBN
cord-268139-tgpsu4qz	364	8	in	in	IN
cord-268139-tgpsu4qz	364	9	this	this	DT
cord-268139-tgpsu4qz	364	10	study	study	NN
cord-268139-tgpsu4qz	364	11	also	also	RB
cord-268139-tgpsu4qz	364	12	detects	detect	VBZ
cord-268139-tgpsu4qz	364	13	the	the	DT
cord-268139-tgpsu4qz	364	14	80-kDa	80-kda	CD
cord-268139-tgpsu4qz	365	1	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	365	2	-nsp2	-nsp2	NNP
cord-268139-tgpsu4qz	365	3	protein	protein	NN
cord-268139-tgpsu4qz	365	4	,	,	,
cord-268139-tgpsu4qz	365	5	we	-PRON-	PRP
cord-268139-tgpsu4qz	365	6	were	be	VBD
cord-268139-tgpsu4qz	365	7	unable	unable	JJ
cord-268139-tgpsu4qz	365	8	to	to	TO
cord-268139-tgpsu4qz	365	9	precisely	precisely	RB
cord-268139-tgpsu4qz	365	10	determine	determine	VB
cord-268139-tgpsu4qz	365	11	whether	whether	IN
cord-268139-tgpsu4qz	365	12	the	the	DT
cord-268139-tgpsu4qz	365	13	14-kDa	14-kda	CD
cord-268139-tgpsu4qz	365	14	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	365	15	protein	protein	NN
cord-268139-tgpsu4qz	365	16	is	be	VBZ
cord-268139-tgpsu4qz	365	17	capable	capable	JJ
cord-268139-tgpsu4qz	365	18	of	of	IN
cord-268139-tgpsu4qz	365	19	localizing	localize	VBG
cord-268139-tgpsu4qz	365	20	to	to	IN
cord-268139-tgpsu4qz	365	21	virion	virion	NN
cord-268139-tgpsu4qz	365	22	assembly	assembly	NN
cord-268139-tgpsu4qz	365	23	sites	site	NNS
cord-268139-tgpsu4qz	365	24	late	late	RB
cord-268139-tgpsu4qz	365	25	in	in	IN
cord-268139-tgpsu4qz	365	26	infection	infection	NN
cord-268139-tgpsu4qz	365	27	.	.	.
cord-268139-tgpsu4qz	366	1	Nonetheless	nonetheless	RB
cord-268139-tgpsu4qz	366	2	,	,	,
cord-268139-tgpsu4qz	366	3	it	-PRON-	PRP
cord-268139-tgpsu4qz	366	4	will	will	MD
cord-268139-tgpsu4qz	366	5	be	be	VB
cord-268139-tgpsu4qz	366	6	valuable	valuable	JJ
cord-268139-tgpsu4qz	366	7	to	to	TO
cord-268139-tgpsu4qz	366	8	determine	determine	VB
cord-268139-tgpsu4qz	366	9	whether	whether	IN
cord-268139-tgpsu4qz	366	10	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	366	11	,	,	,
cord-268139-tgpsu4qz	366	12	or	or	CC
cord-268139-tgpsu4qz	366	13	any	any	DT
cord-268139-tgpsu4qz	366	14	of	of	IN
cord-268139-tgpsu4qz	366	15	the	the	DT
cord-268139-tgpsu4qz	366	16	mutant	mutant	JJ
cord-268139-tgpsu4qz	366	17	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	366	18	proteins	protein	NNS
cord-268139-tgpsu4qz	366	19	,	,	,
cord-268139-tgpsu4qz	366	20	exhibit	exhibit	VBP
cord-268139-tgpsu4qz	366	21	differences	difference	NNS
cord-268139-tgpsu4qz	366	22	in	in	IN
cord-268139-tgpsu4qz	366	23	protein	protein	NN
cord-268139-tgpsu4qz	366	24	localization	localization	NN
cord-268139-tgpsu4qz	366	25	during	during	IN
cord-268139-tgpsu4qz	366	26	late	late	JJ
cord-268139-tgpsu4qz	366	27	times	time	NNS
cord-268139-tgpsu4qz	366	28	of	of	IN
cord-268139-tgpsu4qz	366	29	infection	infection	NN
cord-268139-tgpsu4qz	366	30	.	.	.
cord-268139-tgpsu4qz	367	1	In	in	IN
cord-268139-tgpsu4qz	367	2	summary	summary	NN
cord-268139-tgpsu4qz	367	3	,	,	,
cord-268139-tgpsu4qz	367	4	the	the	DT
cord-268139-tgpsu4qz	367	5	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	367	6	of	of	IN
cord-268139-tgpsu4qz	367	7	these	these	DT
cord-268139-tgpsu4qz	367	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	367	9	mutant	mutant	JJ
cord-268139-tgpsu4qz	367	10	viruses	virus	NNS
cord-268139-tgpsu4qz	367	11	reveal	reveal	VBP
cord-268139-tgpsu4qz	367	12	new	new	JJ
cord-268139-tgpsu4qz	367	13	information	information	NN
cord-268139-tgpsu4qz	367	14	about	about	IN
cord-268139-tgpsu4qz	367	15	the	the	DT
cord-268139-tgpsu4qz	367	16	possible	possible	JJ
cord-268139-tgpsu4qz	367	17	roles	role	NNS
cord-268139-tgpsu4qz	367	18	of	of	IN
cord-268139-tgpsu4qz	367	19	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	367	20	during	during	IN
cord-268139-tgpsu4qz	367	21	viral	viral	JJ
cord-268139-tgpsu4qz	367	22	replication	replication	NN
cord-268139-tgpsu4qz	367	23	.	.	.
cord-268139-tgpsu4qz	368	1	However	however	RB
cord-268139-tgpsu4qz	368	2	,	,	,
cord-268139-tgpsu4qz	368	3	it	-PRON-	PRP
cord-268139-tgpsu4qz	368	4	is	be	VBZ
cord-268139-tgpsu4qz	368	5	important	important	JJ
cord-268139-tgpsu4qz	368	6	to	to	TO
cord-268139-tgpsu4qz	368	7	note	note	VB
cord-268139-tgpsu4qz	368	8	the	the	DT
cord-268139-tgpsu4qz	368	9	limitations	limitation	NNS
cord-268139-tgpsu4qz	368	10	of	of	IN
cord-268139-tgpsu4qz	368	11	the	the	DT
cord-268139-tgpsu4qz	368	12	reverse	reverse	JJ
cord-268139-tgpsu4qz	368	13	genetic	genetic	JJ
cord-268139-tgpsu4qz	368	14	approach	approach	NN
cord-268139-tgpsu4qz	368	15	used	use	VBN
cord-268139-tgpsu4qz	368	16	in	in	IN
cord-268139-tgpsu4qz	368	17	this	this	DT
cord-268139-tgpsu4qz	368	18	study	study	NN
cord-268139-tgpsu4qz	368	19	and	and	CC
cord-268139-tgpsu4qz	368	20	to	to	TO
cord-268139-tgpsu4qz	368	21	explore	explore	VB
cord-268139-tgpsu4qz	368	22	alternative	alternative	JJ
cord-268139-tgpsu4qz	368	23	explanations	explanation	NNS
cord-268139-tgpsu4qz	368	24	of	of	IN
cord-268139-tgpsu4qz	368	25	the	the	DT
cord-268139-tgpsu4qz	368	26	available	available	JJ
cord-268139-tgpsu4qz	368	27	data	datum	NNS
cord-268139-tgpsu4qz	368	28	.	.	.
cord-268139-tgpsu4qz	369	1	For	for	IN
cord-268139-tgpsu4qz	369	2	example	example	NN
cord-268139-tgpsu4qz	369	3	,	,	,
cord-268139-tgpsu4qz	369	4	it	-PRON-	PRP
cord-268139-tgpsu4qz	369	5	has	have	VBZ
cord-268139-tgpsu4qz	369	6	been	be	VBN
cord-268139-tgpsu4qz	369	7	reported	report	VBN
cord-268139-tgpsu4qz	369	8	that	that	IN
cord-268139-tgpsu4qz	369	9	exogenous	exogenous	JJ
cord-268139-tgpsu4qz	369	10	MHV	MHV	NNP
cord-268139-tgpsu4qz	369	11	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	369	12	expression	expression	NN
cord-268139-tgpsu4qz	369	13	induces	induce	VBZ
cord-268139-tgpsu4qz	369	14	cell	cell	NN
cord-268139-tgpsu4qz	369	15	-	-	HYPH
cord-268139-tgpsu4qz	369	16	cycle	cycle	NN
cord-268139-tgpsu4qz	369	17	arrest	arrest	NN
cord-268139-tgpsu4qz	369	18	(	(	-LRB-
cord-268139-tgpsu4qz	369	19	Chen	Chen	NNP
cord-268139-tgpsu4qz	369	20	et	et	NNP
cord-268139-tgpsu4qz	369	21	al	al	NNP
cord-268139-tgpsu4qz	369	22	.	.	NNP
cord-268139-tgpsu4qz	369	23	,	,	,
cord-268139-tgpsu4qz	369	24	2004	2004	CD
cord-268139-tgpsu4qz	369	25	)	)	-RRB-
cord-268139-tgpsu4qz	369	26	.	.	.
cord-268139-tgpsu4qz	370	1	Therefore	therefore	RB
cord-268139-tgpsu4qz	370	2	,	,	,
cord-268139-tgpsu4qz	370	3	it	-PRON-	PRP
cord-268139-tgpsu4qz	370	4	is	be	VBZ
cord-268139-tgpsu4qz	370	5	also	also	RB
cord-268139-tgpsu4qz	370	6	possible	possible	JJ
cord-268139-tgpsu4qz	370	7	that	that	IN
cord-268139-tgpsu4qz	370	8	the	the	DT
cord-268139-tgpsu4qz	370	9	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	370	10	mutations	mutation	NNS
cord-268139-tgpsu4qz	370	11	have	have	VBP
cord-268139-tgpsu4qz	370	12	an	an	DT
cord-268139-tgpsu4qz	370	13	indirect	indirect	JJ
cord-268139-tgpsu4qz	370	14	effect	effect	NN
cord-268139-tgpsu4qz	370	15	on	on	IN
cord-268139-tgpsu4qz	370	16	MHV	MHV	NNP
cord-268139-tgpsu4qz	370	17	replication	replication	NN
cord-268139-tgpsu4qz	370	18	as	as	IN
cord-268139-tgpsu4qz	370	19	a	a	DT
cord-268139-tgpsu4qz	370	20	result	result	NN
cord-268139-tgpsu4qz	370	21	of	of	IN
cord-268139-tgpsu4qz	370	22	cell	cell	NN
cord-268139-tgpsu4qz	370	23	biological	biological	JJ
cord-268139-tgpsu4qz	370	24	changes	change	NNS
cord-268139-tgpsu4qz	370	25	during	during	IN
cord-268139-tgpsu4qz	370	26	infection	infection	NN
cord-268139-tgpsu4qz	370	27	.	.	.
cord-268139-tgpsu4qz	371	1	Moreover	moreover	RB
cord-268139-tgpsu4qz	371	2	,	,	,
cord-268139-tgpsu4qz	371	3	while	while	IN
cord-268139-tgpsu4qz	371	4	it	-PRON-	PRP
cord-268139-tgpsu4qz	371	5	is	be	VBZ
cord-268139-tgpsu4qz	371	6	clear	clear	JJ
cord-268139-tgpsu4qz	371	7	that	that	IN
cord-268139-tgpsu4qz	371	8	some	some	DT
cord-268139-tgpsu4qz	371	9	of	of	IN
cord-268139-tgpsu4qz	371	10	the	the	DT
cord-268139-tgpsu4qz	371	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	371	12	mutants	mutant	NNS
cord-268139-tgpsu4qz	371	13	have	have	VBP
cord-268139-tgpsu4qz	371	14	differences	difference	NNS
cord-268139-tgpsu4qz	371	15	in	in	IN
cord-268139-tgpsu4qz	371	16	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	371	17	confirmation	confirmation	NN
cord-268139-tgpsu4qz	371	18	and	and	CC
cord-268139-tgpsu4qz	371	19	interactions	interaction	NNS
cord-268139-tgpsu4qz	371	20	,	,	,
cord-268139-tgpsu4qz	371	21	it	-PRON-	PRP
cord-268139-tgpsu4qz	371	22	is	be	VBZ
cord-268139-tgpsu4qz	371	23	possible	possible	JJ
cord-268139-tgpsu4qz	371	24	that	that	IN
cord-268139-tgpsu4qz	371	25	uncharacterized	uncharacterized	JJ
cord-268139-tgpsu4qz	371	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	371	27	structural	structural	JJ
cord-268139-tgpsu4qz	371	28	elements	element	NNS
cord-268139-tgpsu4qz	371	29	have	have	VBP
cord-268139-tgpsu4qz	371	30	also	also	RB
cord-268139-tgpsu4qz	371	31	been	be	VBN
cord-268139-tgpsu4qz	371	32	disrupted	disrupt	VBN
cord-268139-tgpsu4qz	371	33	as	as	IN
cord-268139-tgpsu4qz	371	34	a	a	DT
cord-268139-tgpsu4qz	371	35	result	result	NN
cord-268139-tgpsu4qz	371	36	of	of	IN
cord-268139-tgpsu4qz	371	37	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	371	38	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	371	39	resulting	result	VBG
cord-268139-tgpsu4qz	371	40	in	in	IN
cord-268139-tgpsu4qz	371	41	changes	change	NNS
cord-268139-tgpsu4qz	371	42	in	in	IN
cord-268139-tgpsu4qz	371	43	viral	viral	JJ
cord-268139-tgpsu4qz	371	44	RNA	RNA	NNP
cord-268139-tgpsu4qz	371	45	synthesis	synthesis	NN
cord-268139-tgpsu4qz	371	46	and	and	CC
cord-268139-tgpsu4qz	371	47	replication	replication	NN
cord-268139-tgpsu4qz	371	48	.	.	.
cord-268139-tgpsu4qz	372	1	Furthermore	furthermore	RB
cord-268139-tgpsu4qz	372	2	,	,	,
cord-268139-tgpsu4qz	372	3	in	in	IN
cord-268139-tgpsu4qz	372	4	the	the	DT
cord-268139-tgpsu4qz	372	5	absence	absence	NN
cord-268139-tgpsu4qz	372	6	of	of	IN
cord-268139-tgpsu4qz	372	7	sequencing	sequence	VBG
cord-268139-tgpsu4qz	372	8	the	the	DT
cord-268139-tgpsu4qz	372	9	entire	entire	JJ
cord-268139-tgpsu4qz	372	10	32-kb	32-kb	CD
cord-268139-tgpsu4qz	372	11	genomes	genome	NNS
cord-268139-tgpsu4qz	372	12	of	of	IN
cord-268139-tgpsu4qz	372	13	the	the	DT
cord-268139-tgpsu4qz	372	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	372	15	mutant	mutant	JJ
cord-268139-tgpsu4qz	372	16	viruses	virus	NNS
cord-268139-tgpsu4qz	372	17	,	,	,
cord-268139-tgpsu4qz	372	18	the	the	DT
cord-268139-tgpsu4qz	372	19	possibility	possibility	NN
cord-268139-tgpsu4qz	372	20	that	that	IN
cord-268139-tgpsu4qz	372	21	other	other	JJ
cord-268139-tgpsu4qz	372	22	mutations	mutation	NNS
cord-268139-tgpsu4qz	372	23	contribute	contribute	VBP
cord-268139-tgpsu4qz	372	24	to	to	IN
cord-268139-tgpsu4qz	372	25	the	the	DT
cord-268139-tgpsu4qz	372	26	described	describe	VBN
cord-268139-tgpsu4qz	372	27	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	372	28	can	can	MD
cord-268139-tgpsu4qz	372	29	not	not	RB
cord-268139-tgpsu4qz	372	30	be	be	VB
cord-268139-tgpsu4qz	372	31	excluded	exclude	VBN
cord-268139-tgpsu4qz	372	32	.	.	.
cord-268139-tgpsu4qz	373	1	To	to	TO
cord-268139-tgpsu4qz	373	2	rule	rule	VB
cord-268139-tgpsu4qz	373	3	out	out	RP
cord-268139-tgpsu4qz	373	4	the	the	DT
cord-268139-tgpsu4qz	373	5	contributions	contribution	NNS
cord-268139-tgpsu4qz	373	6	of	of	IN
cord-268139-tgpsu4qz	373	7	other	other	JJ
cord-268139-tgpsu4qz	373	8	mutations	mutation	NNS
cord-268139-tgpsu4qz	373	9	or	or	CC
cord-268139-tgpsu4qz	373	10	RNA	RNA	NNP
cord-268139-tgpsu4qz	373	11	elements	element	NNS
cord-268139-tgpsu4qz	373	12	,	,	,
cord-268139-tgpsu4qz	373	13	wild	wild	JJ
cord-268139-tgpsu4qz	373	14	-	-	HYPH
cord-268139-tgpsu4qz	373	15	type	type	NN
cord-268139-tgpsu4qz	373	16	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	373	17	expressed	express	VBN
cord-268139-tgpsu4qz	373	18	in	in	IN
cord-268139-tgpsu4qz	373	19	cells	cell	NNS
cord-268139-tgpsu4qz	373	20	must	must	MD
cord-268139-tgpsu4qz	373	21	complement	complement	VB
cord-268139-tgpsu4qz	373	22	the	the	DT
cord-268139-tgpsu4qz	373	23	defects	defect	NNS
cord-268139-tgpsu4qz	373	24	of	of	IN
cord-268139-tgpsu4qz	373	25	these	these	DT
cord-268139-tgpsu4qz	373	26	viruses	virus	NNS
cord-268139-tgpsu4qz	373	27	.	.	.
cord-268139-tgpsu4qz	374	1	While	while	IN
cord-268139-tgpsu4qz	374	2	ideal	ideal	JJ
cord-268139-tgpsu4qz	374	3	,	,	,
cord-268139-tgpsu4qz	374	4	trans	trans	NN
cord-268139-tgpsu4qz	374	5	-	-	NN
cord-268139-tgpsu4qz	374	6	complementation	complementation	NN
cord-268139-tgpsu4qz	374	7	using	use	VBG
cord-268139-tgpsu4qz	374	8	protein	protein	NN
cord-268139-tgpsu4qz	374	9	expression	expression	NN
cord-268139-tgpsu4qz	374	10	has	have	VBZ
cord-268139-tgpsu4qz	374	11	not	not	RB
cord-268139-tgpsu4qz	374	12	been	be	VBN
cord-268139-tgpsu4qz	374	13	successful	successful	JJ
cord-268139-tgpsu4qz	374	14	for	for	IN
cord-268139-tgpsu4qz	374	15	coronaviruses	coronaviruse	NNS
cord-268139-tgpsu4qz	374	16	.	.	.
cord-268139-tgpsu4qz	375	1	In	in	IN
cord-268139-tgpsu4qz	375	2	fact	fact	NN
cord-268139-tgpsu4qz	375	3	,	,	,
cord-268139-tgpsu4qz	375	4	efficient	efficient	JJ
cord-268139-tgpsu4qz	375	5	complementation	complementation	NN
cord-268139-tgpsu4qz	375	6	for	for	IN
cord-268139-tgpsu4qz	375	7	other	other	JJ
cord-268139-tgpsu4qz	375	8	positive	positive	JJ
cord-268139-tgpsu4qz	375	9	-	-	HYPH
cord-268139-tgpsu4qz	375	10	strand	strand	NN
cord-268139-tgpsu4qz	375	11	RNA	RNA	NNP
cord-268139-tgpsu4qz	375	12	viruses	virus	NNS
cord-268139-tgpsu4qz	375	13	has	have	VBZ
cord-268139-tgpsu4qz	375	14	required	require	VBN
cord-268139-tgpsu4qz	375	15	the	the	DT
cord-268139-tgpsu4qz	375	16	development	development	NN
cord-268139-tgpsu4qz	375	17	of	of	IN
cord-268139-tgpsu4qz	375	18	replicon	replicon	NN
cord-268139-tgpsu4qz	375	19	systems	system	NNS
cord-268139-tgpsu4qz	375	20	to	to	TO
cord-268139-tgpsu4qz	375	21	facilitate	facilitate	VB
cord-268139-tgpsu4qz	375	22	viral	viral	JJ
cord-268139-tgpsu4qz	375	23	replication	replication	NN
cord-268139-tgpsu4qz	375	24	complex	complex	JJ
cord-268139-tgpsu4qz	375	25	formation	formation	NN
cord-268139-tgpsu4qz	376	1	(	(	-LRB-
cord-268139-tgpsu4qz	376	2	Appel	Appel	NNP
cord-268139-tgpsu4qz	376	3	et	et	FW
cord-268139-tgpsu4qz	376	4	al	al	NNP
cord-268139-tgpsu4qz	376	5	.	.	.
cord-268139-tgpsu4qz	377	1	,	,	,
cord-268139-tgpsu4qz	377	2	2005	2005	CD
cord-268139-tgpsu4qz	377	3	;	;	:
cord-268139-tgpsu4qz	377	4	Grassmann	Grassmann	NNP
cord-268139-tgpsu4qz	377	5	et	et	NNP
cord-268139-tgpsu4qz	377	6	al	al	NNP
cord-268139-tgpsu4qz	377	7	.	.	NNP
cord-268139-tgpsu4qz	377	8	,	,	,
cord-268139-tgpsu4qz	377	9	2001	2001	CD
cord-268139-tgpsu4qz	377	10	;	;	:
cord-268139-tgpsu4qz	377	11	Khromykh	Khromykh	NNP
cord-268139-tgpsu4qz	377	12	et	et	NNP
cord-268139-tgpsu4qz	377	13	al	al	NNP
cord-268139-tgpsu4qz	377	14	.	.	NNP
cord-268139-tgpsu4qz	377	15	,	,	,
cord-268139-tgpsu4qz	377	16	1998	1998	CD
cord-268139-tgpsu4qz	377	17	;	;	:
cord-268139-tgpsu4qz	377	18	Lindenbach	Lindenbach	NNP
cord-268139-tgpsu4qz	377	19	and	and	CC
cord-268139-tgpsu4qz	377	20	Rice	Rice	NNP
cord-268139-tgpsu4qz	377	21	,	,	,
cord-268139-tgpsu4qz	377	22	1997	1997	CD
cord-268139-tgpsu4qz	377	23	;	;	:
cord-268139-tgpsu4qz	377	24	Liu	Liu	NNP
cord-268139-tgpsu4qz	377	25	et	et	NNP
cord-268139-tgpsu4qz	377	26	al	al	NNP
cord-268139-tgpsu4qz	377	27	.	.	NNP
cord-268139-tgpsu4qz	377	28	,	,	,
cord-268139-tgpsu4qz	377	29	2002	2002	CD
cord-268139-tgpsu4qz	377	30	)	)	-RRB-
cord-268139-tgpsu4qz	377	31	.	.	.
cord-268139-tgpsu4qz	378	1	Despite	despite	IN
cord-268139-tgpsu4qz	378	2	these	these	DT
cord-268139-tgpsu4qz	378	3	limitations	limitation	NNS
cord-268139-tgpsu4qz	378	4	,	,	,
cord-268139-tgpsu4qz	378	5	this	this	DT
cord-268139-tgpsu4qz	378	6	is	be	VBZ
cord-268139-tgpsu4qz	378	7	the	the	DT
cord-268139-tgpsu4qz	378	8	first	first	JJ
cord-268139-tgpsu4qz	378	9	report	report	NN
cord-268139-tgpsu4qz	378	10	of	of	IN
cord-268139-tgpsu4qz	378	11	detailed	detailed	JJ
cord-268139-tgpsu4qz	378	12	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	378	13	of	of	IN
cord-268139-tgpsu4qz	378	14	an	an	DT
cord-268139-tgpsu4qz	378	15	MHV	MHV	NNP
cord-268139-tgpsu4qz	378	16	gene	gene	NN
cord-268139-tgpsu4qz	378	17	1	1	CD
cord-268139-tgpsu4qz	378	18	nsp	nsp	NN
cord-268139-tgpsu4qz	378	19	-	-	HYPH
cord-268139-tgpsu4qz	378	20	coding	code	VBG
cord-268139-tgpsu4qz	378	21	region	region	NN
cord-268139-tgpsu4qz	378	22	within	within	IN
cord-268139-tgpsu4qz	378	23	the	the	DT
cord-268139-tgpsu4qz	378	24	context	context	NN
cord-268139-tgpsu4qz	378	25	of	of	IN
cord-268139-tgpsu4qz	378	26	a	a	DT
cord-268139-tgpsu4qz	378	27	virus	virus	NN
cord-268139-tgpsu4qz	378	28	.	.	.
cord-268139-tgpsu4qz	379	1	These	these	DT
cord-268139-tgpsu4qz	379	2	results	result	NNS
cord-268139-tgpsu4qz	379	3	will	will	MD
cord-268139-tgpsu4qz	379	4	enhance	enhance	VB
cord-268139-tgpsu4qz	379	5	our	-PRON-	PRP$
cord-268139-tgpsu4qz	379	6	knowledge	knowledge	NN
cord-268139-tgpsu4qz	379	7	of	of	IN
cord-268139-tgpsu4qz	379	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	379	9	replication	replication	NN
cord-268139-tgpsu4qz	379	10	determinants	determinant	NNS
cord-268139-tgpsu4qz	379	11	,	,	,
cord-268139-tgpsu4qz	379	12	and	and	CC
cord-268139-tgpsu4qz	379	13	future	future	JJ
cord-268139-tgpsu4qz	379	14	analysis	analysis	NN
cord-268139-tgpsu4qz	379	15	of	of	IN
cord-268139-tgpsu4qz	379	16	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	379	17	mutant	mutant	JJ
cord-268139-tgpsu4qz	379	18	viruses	virus	NNS
cord-268139-tgpsu4qz	379	19	will	will	MD
cord-268139-tgpsu4qz	379	20	provide	provide	VB
cord-268139-tgpsu4qz	379	21	greater	great	JJR
cord-268139-tgpsu4qz	379	22	insight	insight	NN
cord-268139-tgpsu4qz	379	23	into	into	IN
cord-268139-tgpsu4qz	379	24	the	the	DT
cord-268139-tgpsu4qz	379	25	function	function	NN
cord-268139-tgpsu4qz	379	26	of	of	IN
cord-268139-tgpsu4qz	379	27	RNA	RNA	NNP
cord-268139-tgpsu4qz	379	28	and	and	CC
cord-268139-tgpsu4qz	379	29	protein	protein	NN
cord-268139-tgpsu4qz	379	30	elements	element	NNS
cord-268139-tgpsu4qz	379	31	within	within	IN
cord-268139-tgpsu4qz	379	32	the	the	DT
cord-268139-tgpsu4qz	379	33	5V	5v	JJ
cord-268139-tgpsu4qz	379	34	end	end	NN
cord-268139-tgpsu4qz	379	35	of	of	IN
cord-268139-tgpsu4qz	379	36	gene	gene	NN
cord-268139-tgpsu4qz	379	37	1	1	CD
cord-268139-tgpsu4qz	379	38	.	.	.
cord-268139-tgpsu4qz	380	1	Even	even	RB
cord-268139-tgpsu4qz	380	2	more	more	JJR
cord-268139-tgpsu4qz	380	3	,	,	,
cord-268139-tgpsu4qz	380	4	studies	study	NNS
cord-268139-tgpsu4qz	380	5	with	with	IN
cord-268139-tgpsu4qz	380	6	these	these	DT
cord-268139-tgpsu4qz	380	7	engineered	engineer	VBN
cord-268139-tgpsu4qz	380	8	mutant	mutant	JJ
cord-268139-tgpsu4qz	380	9	viruses	virus	NNS
cord-268139-tgpsu4qz	380	10	are	be	VBP
cord-268139-tgpsu4qz	380	11	expected	expect	VBN
cord-268139-tgpsu4qz	380	12	to	to	TO
cord-268139-tgpsu4qz	380	13	contribute	contribute	VB
cord-268139-tgpsu4qz	380	14	to	to	IN
cord-268139-tgpsu4qz	380	15	understanding	understand	VBG
cord-268139-tgpsu4qz	380	16	the	the	DT
cord-268139-tgpsu4qz	380	17	replication	replication	NN
cord-268139-tgpsu4qz	380	18	strategies	strategy	NNS
cord-268139-tgpsu4qz	380	19	of	of	IN
cord-268139-tgpsu4qz	380	20	other	other	JJ
cord-268139-tgpsu4qz	380	21	coronaviruses	coronaviruse	NNS
cord-268139-tgpsu4qz	380	22	,	,	,
cord-268139-tgpsu4qz	380	23	such	such	JJ
cord-268139-tgpsu4qz	380	24	as	as	IN
cord-268139-tgpsu4qz	380	25	SARS	SARS	NNP
cord-268139-tgpsu4qz	380	26	-	-	HYPH
cord-268139-tgpsu4qz	380	27	CoV.	CoV.	NNP
cord-268139-tgpsu4qz	380	28	Delayed	delayed	JJ
cord-268139-tgpsu4qz	380	29	brain	brain	NN
cord-268139-tgpsu4qz	380	30	tumor	tumor	NN
cord-268139-tgpsu4qz	380	31	cells	cell	NNS
cord-268139-tgpsu4qz	380	32	selected	select	VBN
cord-268139-tgpsu4qz	380	33	for	for	IN
cord-268139-tgpsu4qz	380	34	high	high	JJ
cord-268139-tgpsu4qz	380	35	-	-	HYPH
cord-268139-tgpsu4qz	380	36	level	level	NN
cord-268139-tgpsu4qz	380	37	expression	expression	NN
cord-268139-tgpsu4qz	380	38	of	of	IN
cord-268139-tgpsu4qz	380	39	the	the	DT
cord-268139-tgpsu4qz	380	40	MHV	MHV	NNP
cord-268139-tgpsu4qz	380	41	receptor	receptor	NN
cord-268139-tgpsu4qz	380	42	carcino	carcino	NN
cord-268139-tgpsu4qz	380	43	-	-	HYPH
cord-268139-tgpsu4qz	380	44	embryonic	embryonic	JJ
cord-268139-tgpsu4qz	380	45	antigen	antigen	NN
cord-268139-tgpsu4qz	380	46	cell	cell	NN
cord-268139-tgpsu4qz	380	47	adhesion	adhesion	NN
cord-268139-tgpsu4qz	380	48	molecule-1	molecule-1	NNP
cord-268139-tgpsu4qz	381	1	(	(	-LRB-
cord-268139-tgpsu4qz	381	2	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	381	3	)	)	-RRB-
cord-268139-tgpsu4qz	382	1	(	(	-LRB-
cord-268139-tgpsu4qz	382	2	Chen	Chen	NNP
cord-268139-tgpsu4qz	382	3	et	et	NNP
cord-268139-tgpsu4qz	382	4	al	al	NNP
cord-268139-tgpsu4qz	382	5	.	.	NNP
cord-268139-tgpsu4qz	382	6	,	,	,
cord-268139-tgpsu4qz	382	7	1997	1997	CD
cord-268139-tgpsu4qz	382	8	;	;	:
cord-268139-tgpsu4qz	382	9	Hirano	Hirano	NNP
cord-268139-tgpsu4qz	382	10	et	et	NNP
cord-268139-tgpsu4qz	382	11	al	al	NNP
cord-268139-tgpsu4qz	382	12	.	.	NNP
cord-268139-tgpsu4qz	382	13	,	,	,
cord-268139-tgpsu4qz	382	14	1976	1976	CD
cord-268139-tgpsu4qz	382	15	;	;	:
cord-268139-tgpsu4qz	382	16	Yount	Yount	NNP
cord-268139-tgpsu4qz	382	17	et	et	NNP
cord-268139-tgpsu4qz	382	18	al	al	NNP
cord-268139-tgpsu4qz	382	19	.	.	NNP
cord-268139-tgpsu4qz	382	20	,	,	,
cord-268139-tgpsu4qz	382	21	2002	2002	CD
cord-268139-tgpsu4qz	382	22	)	)	-RRB-
cord-268139-tgpsu4qz	382	23	and	and	CC
cord-268139-tgpsu4qz	383	1	baby	baby	NNP
cord-268139-tgpsu4qz	383	2	hamster	hamster	NNP
cord-268139-tgpsu4qz	383	3	kidney-21	kidney-21	NNP
cord-268139-tgpsu4qz	383	4	cells	cell	NNS
cord-268139-tgpsu4qz	383	5	expressing	express	VBG
cord-268139-tgpsu4qz	383	6	the	the	DT
cord-268139-tgpsu4qz	383	7	MHV	MHV	NNP
cord-268139-tgpsu4qz	383	8	receptor	receptor	NN
cord-268139-tgpsu4qz	383	9	(	(	-LRB-
cord-268139-tgpsu4qz	383	10	BHK	BHK	NNP
cord-268139-tgpsu4qz	383	11	-	-	HYPH
cord-268139-tgpsu4qz	383	12	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	383	13	)	)	-RRB-
cord-268139-tgpsu4qz	384	1	(	(	-LRB-
cord-268139-tgpsu4qz	384	2	Chen	Chen	NNP
cord-268139-tgpsu4qz	384	3	et	et	NNP
cord-268139-tgpsu4qz	384	4	al	al	NNP
cord-268139-tgpsu4qz	384	5	.	.	NNP
cord-268139-tgpsu4qz	384	6	,	,	,
cord-268139-tgpsu4qz	384	7	1997	1997	CD
cord-268139-tgpsu4qz	384	8	;	;	:
cord-268139-tgpsu4qz	384	9	Yount	Yount	NNP
cord-268139-tgpsu4qz	384	10	et	et	NNP
cord-268139-tgpsu4qz	384	11	al	al	NNP
cord-268139-tgpsu4qz	384	12	.	.	NNP
cord-268139-tgpsu4qz	384	13	,	,	,
cord-268139-tgpsu4qz	384	14	2002	2002	CD
cord-268139-tgpsu4qz	384	15	)	)	-RRB-
cord-268139-tgpsu4qz	384	16	were	be	VBD
cord-268139-tgpsu4qz	384	17	grown	grow	VBN
cord-268139-tgpsu4qz	384	18	in	in	IN
cord-268139-tgpsu4qz	384	19	Dulbecco	Dulbecco	NNP
cord-268139-tgpsu4qz	384	20	's	's	POS
cord-268139-tgpsu4qz	384	21	modified	modify	VBN
cord-268139-tgpsu4qz	384	22	Eagle	Eagle	NNP
cord-268139-tgpsu4qz	384	23	medium	medium	NN
cord-268139-tgpsu4qz	384	24	(	(	-LRB-
cord-268139-tgpsu4qz	384	25	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	384	26	)	)	-RRB-
cord-268139-tgpsu4qz	384	27	(	(	-LRB-
cord-268139-tgpsu4qz	384	28	Gibco	Gibco	NNP
cord-268139-tgpsu4qz	384	29	)	)	-RRB-
cord-268139-tgpsu4qz	384	30	that	that	WDT
cord-268139-tgpsu4qz	384	31	was	be	VBD
cord-268139-tgpsu4qz	384	32	supplemented	supplement	VBN
cord-268139-tgpsu4qz	384	33	with	with	IN
cord-268139-tgpsu4qz	384	34	10	10	CD
cord-268139-tgpsu4qz	384	35	%	%	NN
cord-268139-tgpsu4qz	384	36	heat	heat	NN
cord-268139-tgpsu4qz	384	37	-	-	HYPH
cord-268139-tgpsu4qz	384	38	inactivated	inactivate	VBN
cord-268139-tgpsu4qz	384	39	fetal	fetal	JJ
cord-268139-tgpsu4qz	384	40	calf	calf	NN
cord-268139-tgpsu4qz	384	41	serum	serum	NN
cord-268139-tgpsu4qz	384	42	(	(	-LRB-
cord-268139-tgpsu4qz	384	43	FCS	FCS	NNP
cord-268139-tgpsu4qz	384	44	)	)	-RRB-
cord-268139-tgpsu4qz	384	45	(	(	-LRB-
cord-268139-tgpsu4qz	384	46	Sigma	Sigma	NNP
cord-268139-tgpsu4qz	384	47	)	)	-RRB-
cord-268139-tgpsu4qz	384	48	for	for	IN
cord-268139-tgpsu4qz	384	49	all	all	DT
cord-268139-tgpsu4qz	384	50	experiments	experiment	NNS
cord-268139-tgpsu4qz	384	51	.	.	.
cord-268139-tgpsu4qz	385	1	Medium	medium	NN
cord-268139-tgpsu4qz	385	2	for	for	IN
cord-268139-tgpsu4qz	386	1	BHK	BHK	NNP
cord-268139-tgpsu4qz	386	2	-	-	HYPH
cord-268139-tgpsu4qz	386	3	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	386	4	cells	cell	NNS
cord-268139-tgpsu4qz	386	5	was	be	VBD
cord-268139-tgpsu4qz	386	6	supplemented	supplement	VBN
cord-268139-tgpsu4qz	386	7	with	with	IN
cord-268139-tgpsu4qz	386	8	G418	G418	NNPS
cord-268139-tgpsu4qz	386	9	(	(	-LRB-
cord-268139-tgpsu4qz	386	10	800	800	CD
cord-268139-tgpsu4qz	386	11	Ag/	Ag/	NNP
cord-268139-tgpsu4qz	386	12	ml	ml	NNP
cord-268139-tgpsu4qz	386	13	)	)	-RRB-
cord-268139-tgpsu4qz	386	14	to	to	TO
cord-268139-tgpsu4qz	386	15	select	select	VB
cord-268139-tgpsu4qz	386	16	for	for	IN
cord-268139-tgpsu4qz	386	17	cells	cell	NNS
cord-268139-tgpsu4qz	386	18	expressing	express	VBG
cord-268139-tgpsu4qz	386	19	the	the	DT
cord-268139-tgpsu4qz	386	20	MHV	MHV	NNP
cord-268139-tgpsu4qz	386	21	receptor	receptor	NN
cord-268139-tgpsu4qz	386	22	.	.	.
cord-268139-tgpsu4qz	387	1	Polyclonal	Polyclonal	NNP
cord-268139-tgpsu4qz	387	2	antisera	antisera	NNP
cord-268139-tgpsu4qz	387	3	used	use	VBD
cord-268139-tgpsu4qz	387	4	for	for	IN
cord-268139-tgpsu4qz	387	5	biochemical	biochemical	JJ
cord-268139-tgpsu4qz	387	6	and	and	CC
cord-268139-tgpsu4qz	387	7	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	387	8	experiments	experiment	NNS
cord-268139-tgpsu4qz	387	9	have	have	VBP
cord-268139-tgpsu4qz	387	10	been	be	VBN
cord-268139-tgpsu4qz	387	11	previously	previously	RB
cord-268139-tgpsu4qz	387	12	described	describe	VBN
cord-268139-tgpsu4qz	387	13	.	.	.
cord-268139-tgpsu4qz	388	1	These	these	DT
cord-268139-tgpsu4qz	388	2	include	include	VBP
cord-268139-tgpsu4qz	388	3	guinea	guinea	NN
cord-268139-tgpsu4qz	388	4	pig	pig	NN
cord-268139-tgpsu4qz	388	5	a	a	DT
cord-268139-tgpsu4qz	388	6	-	-	HYPH
cord-268139-tgpsu4qz	388	7	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	388	8	antisera	antisera	NN
cord-268139-tgpsu4qz	388	9	(	(	-LRB-
cord-268139-tgpsu4qz	388	10	GP3	GP3	NNP
cord-268139-tgpsu4qz	388	11	)	)	-RRB-
cord-268139-tgpsu4qz	388	12	;	;	:
cord-268139-tgpsu4qz	388	13	rabbit	rabbit	VB
cord-268139-tgpsu4qz	388	14	a	a	DT
cord-268139-tgpsu4qz	388	15	-	-	HYPH
cord-268139-tgpsu4qz	388	16	nsp2	nsp2	NNP
cord-268139-tgpsu4qz	388	17	antisera	antisera	NNP
cord-268139-tgpsu4qz	388	18	(	(	-LRB-
cord-268139-tgpsu4qz	388	19	VU153	VU153	NNP
cord-268139-tgpsu4qz	388	20	)	)	-RRB-
cord-268139-tgpsu4qz	389	1	(	(	-LRB-
cord-268139-tgpsu4qz	389	2	Sims	Sims	NNP
cord-268139-tgpsu4qz	389	3	et	et	NNP
cord-268139-tgpsu4qz	389	4	al	al	NNP
cord-268139-tgpsu4qz	389	5	.	.	NNP
cord-268139-tgpsu4qz	389	6	,	,	,
cord-268139-tgpsu4qz	389	7	2000	2000	CD
cord-268139-tgpsu4qz	389	8	)	)	-RRB-
cord-268139-tgpsu4qz	389	9	;	;	:
cord-268139-tgpsu4qz	389	10	rabbit	rabbit	VB
cord-268139-tgpsu4qz	389	11	a	a	DT
cord-268139-tgpsu4qz	389	12	-	-	HYPH
cord-268139-tgpsu4qz	389	13	nsp8	nsp8	NN
cord-268139-tgpsu4qz	389	14	antisera	antisera	NN
cord-268139-tgpsu4qz	389	15	(	(	-LRB-
cord-268139-tgpsu4qz	389	16	p1a-22	p1a-22	NNP
cord-268139-tgpsu4qz	389	17	)	)	-RRB-
cord-268139-tgpsu4qz	390	1	(	(	-LRB-
cord-268139-tgpsu4qz	390	2	Bost	Bost	NNP
cord-268139-tgpsu4qz	390	3	et	et	NNP
cord-268139-tgpsu4qz	390	4	al	al	NNP
cord-268139-tgpsu4qz	390	5	.	.	NNP
cord-268139-tgpsu4qz	390	6	,	,	,
cord-268139-tgpsu4qz	390	7	2000	2000	CD
cord-268139-tgpsu4qz	390	8	)	)	-RRB-
cord-268139-tgpsu4qz	390	9	;	;	:
cord-268139-tgpsu4qz	390	10	and	and	CC
cord-268139-tgpsu4qz	390	11	rabbit	rabbit	VB
cord-268139-tgpsu4qz	390	12	a	a	DT
cord-268139-tgpsu4qz	390	13	-	-	HYPH
cord-268139-tgpsu4qz	390	14	MHV	MHV	NNP
cord-268139-tgpsu4qz	390	15	antisera	antisera	NN
cord-268139-tgpsu4qz	390	16	generated	generate	VBN
cord-268139-tgpsu4qz	390	17	against	against	IN
cord-268139-tgpsu4qz	390	18	intact	intact	JJ
cord-268139-tgpsu4qz	390	19	virions	virion	NNS
cord-268139-tgpsu4qz	390	20	.	.	.
cord-268139-tgpsu4qz	391	1	J.	J.	NNP
cord-268139-tgpsu4qz	391	2	Fleming	Fleming	NNP
cord-268139-tgpsu4qz	391	3	(	(	-LRB-
cord-268139-tgpsu4qz	391	4	University	University	NNP
cord-268139-tgpsu4qz	391	5	of	of	IN
cord-268139-tgpsu4qz	391	6	Wisconsin	Wisconsin	NNP
cord-268139-tgpsu4qz	391	7	,	,	,
cord-268139-tgpsu4qz	391	8	Madison	Madison	NNP
cord-268139-tgpsu4qz	391	9	)	)	-RRB-
cord-268139-tgpsu4qz	391	10	kindly	kindly	RB
cord-268139-tgpsu4qz	391	11	provided	provide	VBD
cord-268139-tgpsu4qz	391	12	murine	murine	JJ
cord-268139-tgpsu4qz	391	13	monoclonal	monoclonal	JJ
cord-268139-tgpsu4qz	391	14	antibodies	antibody	NNS
cord-268139-tgpsu4qz	391	15	specific	specific	JJ
cord-268139-tgpsu4qz	391	16	for	for	IN
cord-268139-tgpsu4qz	391	17	the	the	DT
cord-268139-tgpsu4qz	391	18	structural	structural	JJ
cord-268139-tgpsu4qz	391	19	proteins	protein	NNS
cord-268139-tgpsu4qz	391	20	nucleocapsid	nucleocapsid	NN
cord-268139-tgpsu4qz	391	21	(	(	-LRB-
cord-268139-tgpsu4qz	391	22	a	a	NNP
cord-268139-tgpsu4qz	391	23	-	-	HYPH
cord-268139-tgpsu4qz	391	24	N	N	NNP
cord-268139-tgpsu4qz	391	25	,	,	,
cord-268139-tgpsu4qz	391	26	J.3.3	J.3.3	NNP
cord-268139-tgpsu4qz	391	27	)	)	-RRB-
cord-268139-tgpsu4qz	391	28	and	and	CC
cord-268139-tgpsu4qz	391	29	membrane	membrane	NN
cord-268139-tgpsu4qz	391	30	protein	protein	NN
cord-268139-tgpsu4qz	391	31	(	(	-LRB-
cord-268139-tgpsu4qz	391	32	a	a	LS
cord-268139-tgpsu4qz	391	33	-	-	HYPH
cord-268139-tgpsu4qz	391	34	M	M	NNP
cord-268139-tgpsu4qz	391	35	,	,	,
cord-268139-tgpsu4qz	391	36	J.1.3	J.1.3	NNP
cord-268139-tgpsu4qz	391	37	)	)	-RRB-
cord-268139-tgpsu4qz	391	38	.	.	.
cord-268139-tgpsu4qz	392	1	To	to	TO
cord-268139-tgpsu4qz	392	2	delete	delete	VB
cord-268139-tgpsu4qz	392	3	portions	portion	NNS
cord-268139-tgpsu4qz	392	4	of	of	IN
cord-268139-tgpsu4qz	392	5	the	the	DT
cord-268139-tgpsu4qz	392	6	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	392	7	sequence	sequence	NN
cord-268139-tgpsu4qz	392	8	,	,	,
cord-268139-tgpsu4qz	392	9	PCR	PCR	NNP
cord-268139-tgpsu4qz	392	10	was	be	VBD
cord-268139-tgpsu4qz	392	11	performed	perform	VBN
cord-268139-tgpsu4qz	392	12	using	use	VBG
cord-268139-tgpsu4qz	392	13	the	the	DT
cord-268139-tgpsu4qz	392	14	icMHV	icmhv	NN
cord-268139-tgpsu4qz	392	15	-	-	HYPH
cord-268139-tgpsu4qz	392	16	A59	A59	NNP
cord-268139-tgpsu4qz	392	17	fragment	fragment	NN
cord-268139-tgpsu4qz	392	18	A	a	DT
cord-268139-tgpsu4qz	392	19	plasmid	plasmid	NN
cord-268139-tgpsu4qz	392	20	(	(	-LRB-
cord-268139-tgpsu4qz	392	21	pCR	pCR	NNP
cord-268139-tgpsu4qz	392	22	-	-	HYPH
cord-268139-tgpsu4qz	392	23	XL	XL	NNP
cord-268139-tgpsu4qz	392	24	-	-	HYPH
cord-268139-tgpsu4qz	392	25	TopoA	TopoA	NNP
cord-268139-tgpsu4qz	392	26	)	)	-RRB-
cord-268139-tgpsu4qz	392	27	as	as	IN
cord-268139-tgpsu4qz	392	28	template	template	NN
cord-268139-tgpsu4qz	392	29	(	(	-LRB-
cord-268139-tgpsu4qz	392	30	Yount	Yount	NNP
cord-268139-tgpsu4qz	392	31	et	et	NNP
cord-268139-tgpsu4qz	392	32	al	al	NNP
cord-268139-tgpsu4qz	392	33	.	.	NNP
cord-268139-tgpsu4qz	392	34	,	,	,
cord-268139-tgpsu4qz	392	35	2002	2002	CD
cord-268139-tgpsu4qz	392	36	)	)	-RRB-
cord-268139-tgpsu4qz	392	37	.	.	.
cord-268139-tgpsu4qz	393	1	To	to	TO
cord-268139-tgpsu4qz	393	2	generate	generate	VB
cord-268139-tgpsu4qz	393	3	nsp1DFL	nsp1DFL	NNP
cord-268139-tgpsu4qz	393	4	,	,	,
cord-268139-tgpsu4qz	393	5	deleting	delete	VBG
cord-268139-tgpsu4qz	393	6	nearly	nearly	RB
cord-268139-tgpsu4qz	393	7	the	the	DT
cord-268139-tgpsu4qz	393	8	entire	entire	JJ
cord-268139-tgpsu4qz	393	9	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	393	10	sequence	sequence	NN
cord-268139-tgpsu4qz	393	11	(	(	-LRB-
cord-268139-tgpsu4qz	393	12	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	393	13	amino	amino	NN
cord-268139-tgpsu4qz	393	14	acids	acid	NNS
cord-268139-tgpsu4qz	394	1	A	a	DT
cord-268139-tgpsu4qz	394	2	2	2	CD
cord-268139-tgpsu4qz	394	3	through	through	IN
cord-268139-tgpsu4qz	394	4	L	L	NNP
cord-268139-tgpsu4qz	394	5	241	241	CD
cord-268139-tgpsu4qz	394	6	)	)	-RRB-
cord-268139-tgpsu4qz	394	7	,	,	,
cord-268139-tgpsu4qz	394	8	a	a	DT
cord-268139-tgpsu4qz	394	9	PCR	PCR	NNP
cord-268139-tgpsu4qz	394	10	product	product	NN
cord-268139-tgpsu4qz	394	11	was	be	VBD
cord-268139-tgpsu4qz	394	12	amplified	amplify	VBN
cord-268139-tgpsu4qz	394	13	using	use	VBG
cord-268139-tgpsu4qz	394	14	oligodeoxynucleotide	oligodeoxynucleotide	JJ
cord-268139-tgpsu4qz	394	15	primers	primer	NNS
cord-268139-tgpsu4qz	394	16	(	(	-LRB-
cord-268139-tgpsu4qz	394	17	sense	sense	NN
cord-268139-tgpsu4qz	394	18	)	)	-RRB-
cord-268139-tgpsu4qz	394	19	5V	5V	NNP
cord-268139-tgpsu4qz	394	20	-	-	HYPH
cord-268139-tgpsu4qz	394	21	GTT	GTT	NNP
cord-268139-tgpsu4qz	394	22	TAA	TAA	NNP
cord-268139-tgpsu4qz	394	23	ACG	ACG	NNP
cord-268139-tgpsu4qz	394	24	AGA	AGA	NNP
cord-268139-tgpsu4qz	394	25	CAT	cat	NN
cord-268139-tgpsu4qz	394	26	AAT	AAT	NNP
cord-268139-tgpsu4qz	394	27	ACG-3V	ACG-3V	NNP
cord-268139-tgpsu4qz	394	28	and	and	CC
cord-268139-tgpsu4qz	394	29	(	(	-LRB-
cord-268139-tgpsu4qz	394	30	antisense	antisense	NNP
cord-268139-tgpsu4qz	394	31	)	)	-RRB-
cord-268139-tgpsu4qz	395	1	5V	5V	NNP
cord-268139-tgpsu4qz	395	2	-	-	HYPH
cord-268139-tgpsu4qz	395	3	CTT	CTT	NNP
cord-268139-tgpsu4qz	395	4	AAG	AAG	NNP
cord-268139-tgpsu4qz	396	1	CAT	CAT	NNP
cord-268139-tgpsu4qz	396	2	TAT	TAT	NNP
cord-268139-tgpsu4qz	396	3	GCA	GCA	NNP
cord-268139-tgpsu4qz	396	4	ACC	ACC	NNP
cord-268139-tgpsu4qz	396	5	TAT-3V.	tat-3v.	NN
cord-268139-tgpsu4qz	397	1	To	to	TO
cord-268139-tgpsu4qz	397	2	generate	generate	VB
cord-268139-tgpsu4qz	397	3	nsp1DMid	nsp1DMid	NNP
cord-268139-tgpsu4qz	397	4	,	,	,
cord-268139-tgpsu4qz	397	5	deleting	delete	VBG
cord-268139-tgpsu4qz	397	6	the	the	DT
cord-268139-tgpsu4qz	397	7	middle	middle	JJ
cord-268139-tgpsu4qz	397	8	portion	portion	NN
cord-268139-tgpsu4qz	397	9	of	of	IN
cord-268139-tgpsu4qz	397	10	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	397	11	(	(	-LRB-
cord-268139-tgpsu4qz	397	12	Q	Q	NNP
cord-268139-tgpsu4qz	397	13	87	87	CD
cord-268139-tgpsu4qz	397	14	through	through	IN
cord-268139-tgpsu4qz	397	15	N	n	CD
cord-268139-tgpsu4qz	397	16	164	164	CD
cord-268139-tgpsu4qz	397	17	)	)	-RRB-
cord-268139-tgpsu4qz	397	18	,	,	,
cord-268139-tgpsu4qz	397	19	two	two	CD
cord-268139-tgpsu4qz	397	20	separate	separate	JJ
cord-268139-tgpsu4qz	397	21	PCR	PCR	NNP
cord-268139-tgpsu4qz	397	22	products	product	NNS
cord-268139-tgpsu4qz	397	23	were	be	VBD
cord-268139-tgpsu4qz	397	24	amplified	amplify	VBN
cord-268139-tgpsu4qz	397	25	.	.	.
cord-268139-tgpsu4qz	398	1	The	the	DT
cord-268139-tgpsu4qz	398	2	first	first	JJ
cord-268139-tgpsu4qz	398	3	PCR	PCR	NNP
cord-268139-tgpsu4qz	398	4	reaction	reaction	NN
cord-268139-tgpsu4qz	398	5	used	use	VBD
cord-268139-tgpsu4qz	398	6	primers	primer	NNS
cord-268139-tgpsu4qz	398	7	(	(	-LRB-
cord-268139-tgpsu4qz	398	8	sense	sense	NN
cord-268139-tgpsu4qz	398	9	)	)	-RRB-
cord-268139-tgpsu4qz	399	1	5V	5v	JJ
cord-268139-tgpsu4qz	399	2	-	-	HYPH
cord-268139-tgpsu4qz	399	3	CCG	CCG	NNP
cord-268139-tgpsu4qz	399	4	CCG	CCG	NNP
cord-268139-tgpsu4qz	399	5	GCC	gcc	NN
cord-268139-tgpsu4qz	399	6	TGG	TGG	NNP
cord-268139-tgpsu4qz	399	7	TCT	TCT	NNP
cord-268139-tgpsu4qz	399	8	TGT-3V	TGT-3V	NNP
cord-268139-tgpsu4qz	399	9	and	and	CC
cord-268139-tgpsu4qz	399	10	(	(	-LRB-
cord-268139-tgpsu4qz	399	11	antisense	antisense	NNP
cord-268139-tgpsu4qz	399	12	)	)	-RRB-
cord-268139-tgpsu4qz	400	1	5V	5V	NNP
cord-268139-tgpsu4qz	400	2	-	-	HYPH
cord-268139-tgpsu4qz	400	3	GGA	GGA	NNP
cord-268139-tgpsu4qz	400	4	TCC	TCC	NNP
cord-268139-tgpsu4qz	400	5	TCA	TCA	NNP
cord-268139-tgpsu4qz	400	6	TCT	TCT	NNP
cord-268139-tgpsu4qz	400	7	ACA	ACA	NNP
cord-268139-tgpsu4qz	400	8	AA-3V	AA-3V	NNP
cord-268139-tgpsu4qz	400	9	to	to	TO
cord-268139-tgpsu4qz	400	10	amplify	amplify	VB
cord-268139-tgpsu4qz	400	11	sequences	sequence	NNS
cord-268139-tgpsu4qz	400	12	corresponding	correspond	VBG
cord-268139-tgpsu4qz	400	13	to	to	IN
cord-268139-tgpsu4qz	400	14	the	the	DT
cord-268139-tgpsu4qz	400	15	5VUTR	5vutr	CD
cord-268139-tgpsu4qz	400	16	and	and	CC
cord-268139-tgpsu4qz	400	17	the	the	DT
cord-268139-tgpsu4qz	400	18	amino	amino	NN
cord-268139-tgpsu4qz	400	19	-	-	HYPH
cord-268139-tgpsu4qz	400	20	terminal	terminal	NN
cord-268139-tgpsu4qz	400	21	86	86	CD
cord-268139-tgpsu4qz	400	22	residues	residue	NNS
cord-268139-tgpsu4qz	400	23	(	(	-LRB-
cord-268139-tgpsu4qz	400	24	M	M	NNP
cord-268139-tgpsu4qz	400	25	1	1	CD
cord-268139-tgpsu4qz	400	26	through	through	IN
cord-268139-tgpsu4qz	400	27	P	p	NN
cord-268139-tgpsu4qz	400	28	86	86	CD
cord-268139-tgpsu4qz	400	29	)	)	-RRB-
cord-268139-tgpsu4qz	400	30	of	of	IN
cord-268139-tgpsu4qz	400	31	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	400	32	.	.	.
cord-268139-tgpsu4qz	401	1	The	the	DT
cord-268139-tgpsu4qz	401	2	second	second	JJ
cord-268139-tgpsu4qz	401	3	reaction	reaction	NN
cord-268139-tgpsu4qz	401	4	used	use	VBN
cord-268139-tgpsu4qz	401	5	primers	primer	NNS
cord-268139-tgpsu4qz	401	6	(	(	-LRB-
cord-268139-tgpsu4qz	401	7	sense	sense	NN
cord-268139-tgpsu4qz	401	8	)	)	-RRB-
cord-268139-tgpsu4qz	401	9	5V	5v	JJ
cord-268139-tgpsu4qz	401	10	-	-	HYPH
cord-268139-tgpsu4qz	401	11	GAT	GAT	NNP
cord-268139-tgpsu4qz	401	12	CCC	CCC	NNP
cord-268139-tgpsu4qz	401	13	GGC	GGC	NNP
cord-268139-tgpsu4qz	401	14	CGT	CGT	NNP
cord-268139-tgpsu4qz	401	15	TTT	TTT	NNP
cord-268139-tgpsu4qz	401	16	ATA	ATA	NNP
cord-268139-tgpsu4qz	401	17	GGC-3V	GGC-3V	NNP
cord-268139-tgpsu4qz	401	18	and	and	CC
cord-268139-tgpsu4qz	401	19	(	(	-LRB-
cord-268139-tgpsu4qz	401	20	antisense	antisense	NNP
cord-268139-tgpsu4qz	401	21	)	)	-RRB-
cord-268139-tgpsu4qz	401	22	5V	5V	NNP
cord-268139-tgpsu4qz	401	23	-	-	HYPH
cord-268139-tgpsu4qz	401	24	CTT	CTT	NNP
cord-268139-tgpsu4qz	401	25	AAG	AAG	NNP
cord-268139-tgpsu4qz	401	26	AAG	AAG	NNP
cord-268139-tgpsu4qz	401	27	AGC	AGC	NNP
cord-268139-tgpsu4qz	401	28	ATA-3V	ATA-3V	NNP
cord-268139-tgpsu4qz	401	29	to	to	TO
cord-268139-tgpsu4qz	401	30	amplify	amplify	VB
cord-268139-tgpsu4qz	401	31	sequences	sequence	NNS
cord-268139-tgpsu4qz	401	32	corresponding	correspond	VBG
cord-268139-tgpsu4qz	401	33	to	to	IN
cord-268139-tgpsu4qz	401	34	the	the	DT
cord-268139-tgpsu4qz	401	35	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	401	36	-	-	HYPH
cord-268139-tgpsu4qz	401	37	terminal	terminal	NN
cord-268139-tgpsu4qz	401	38	78	78	CD
cord-268139-tgpsu4qz	401	39	residues	residue	NNS
cord-268139-tgpsu4qz	401	40	(	(	-LRB-
cord-268139-tgpsu4qz	401	41	G	G	NNP
cord-268139-tgpsu4qz	401	42	165	165	CD
cord-268139-tgpsu4qz	401	43	through	through	IN
cord-268139-tgpsu4qz	401	44	L	L	NNP
cord-268139-tgpsu4qz	401	45	241	241	CD
cord-268139-tgpsu4qz	401	46	)	)	-RRB-
cord-268139-tgpsu4qz	401	47	.	.	.
cord-268139-tgpsu4qz	402	1	To	to	TO
cord-268139-tgpsu4qz	402	2	generate	generate	VB
cord-268139-tgpsu4qz	402	3	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	402	4	,	,	,
cord-268139-tgpsu4qz	402	5	deleting	delete	VBG
cord-268139-tgpsu4qz	402	6	the	the	DT
cord-268139-tgpsu4qz	402	7	carboxy	carboxy	JJ
cord-268139-tgpsu4qz	402	8	-	-	HYPH
cord-268139-tgpsu4qz	402	9	terminus	terminus	NN
cord-268139-tgpsu4qz	402	10	of	of	IN
cord-268139-tgpsu4qz	402	11	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	402	12	(	(	-LRB-
cord-268139-tgpsu4qz	402	13	K	K	NNP
cord-268139-tgpsu4qz	402	14	124	124	CD
cord-268139-tgpsu4qz	402	15	through	through	IN
cord-268139-tgpsu4qz	402	16	L	L	NNP
cord-268139-tgpsu4qz	402	17	241	241	CD
cord-268139-tgpsu4qz	402	18	)	)	-RRB-
cord-268139-tgpsu4qz	402	19	,	,	,
cord-268139-tgpsu4qz	402	20	a	a	DT
cord-268139-tgpsu4qz	402	21	PCR	PCR	NNP
cord-268139-tgpsu4qz	402	22	product	product	NN
cord-268139-tgpsu4qz	402	23	was	be	VBD
cord-268139-tgpsu4qz	402	24	amplified	amplify	VBN
cord-268139-tgpsu4qz	402	25	using	use	VBG
cord-268139-tgpsu4qz	402	26	primers	primer	NNS
cord-268139-tgpsu4qz	402	27	(	(	-LRB-
cord-268139-tgpsu4qz	402	28	sense	sense	NN
cord-268139-tgpsu4qz	402	29	)	)	-RRB-
cord-268139-tgpsu4qz	402	30	5V	5v	JJ
cord-268139-tgpsu4qz	402	31	-	-	HYPH
cord-268139-tgpsu4qz	402	32	CCG	ccg	NN
cord-268139-tgpsu4qz	402	33	CCG	CCG	NNP
cord-268139-tgpsu4qz	402	34	GCC	gcc	NN
cord-268139-tgpsu4qz	402	35	TGG	TGG	NNP
cord-268139-tgpsu4qz	402	36	TCT	TCT	NNP
cord-268139-tgpsu4qz	402	37	TGT-3V	TGT-3V	NNP
cord-268139-tgpsu4qz	402	38	and	and	CC
cord-268139-tgpsu4qz	402	39	(	(	-LRB-
cord-268139-tgpsu4qz	402	40	antisense	antisense	NNP
cord-268139-tgpsu4qz	402	41	)	)	-RRB-
cord-268139-tgpsu4qz	403	1	5V	5V	NNP
cord-268139-tgpsu4qz	403	2	-	-	HYPH
cord-268139-tgpsu4qz	403	3	CTT	CTT	NNP
cord-268139-tgpsu4qz	403	4	AAG	AAG	NNP
cord-268139-tgpsu4qz	403	5	GGG	GGG	NNP
cord-268139-tgpsu4qz	403	6	AAG	AAG	NNP
cord-268139-tgpsu4qz	403	7	CAC	CAC	NNP
cord-268139-tgpsu4qz	403	8	ACC	ACC	NNP
cord-268139-tgpsu4qz	404	1	CAA-3V.	CAA-3V.	NNP
cord-268139-tgpsu4qz	405	1	All	all	DT
cord-268139-tgpsu4qz	405	2	PCR	PCR	NNP
cord-268139-tgpsu4qz	405	3	products	product	NNS
cord-268139-tgpsu4qz	405	4	were	be	VBD
cord-268139-tgpsu4qz	405	5	ligated	ligate	VBN
cord-268139-tgpsu4qz	405	6	into	into	IN
cord-268139-tgpsu4qz	405	7	pGEM	pgem	JJ
cord-268139-tgpsu4qz	405	8	-	-	HYPH
cord-268139-tgpsu4qz	405	9	T	T	NNP
cord-268139-tgpsu4qz	405	10	-	-	HYPH
cord-268139-tgpsu4qz	405	11	Easy	Easy	NNP
cord-268139-tgpsu4qz	405	12	(	(	-LRB-
cord-268139-tgpsu4qz	405	13	Promega	Promega	NNP
cord-268139-tgpsu4qz	405	14	)	)	-RRB-
cord-268139-tgpsu4qz	405	15	and	and	CC
cord-268139-tgpsu4qz	405	16	sequenced	sequence	VBN
cord-268139-tgpsu4qz	405	17	to	to	TO
cord-268139-tgpsu4qz	405	18	ensure	ensure	VB
cord-268139-tgpsu4qz	405	19	the	the	DT
cord-268139-tgpsu4qz	405	20	fidelity	fidelity	NN
cord-268139-tgpsu4qz	405	21	of	of	IN
cord-268139-tgpsu4qz	405	22	PCR	PCR	NNP
cord-268139-tgpsu4qz	405	23	.	.	.
cord-268139-tgpsu4qz	406	1	Nsp1	nsp1	NN
cord-268139-tgpsu4qz	406	2	sequences	sequence	NNS
cord-268139-tgpsu4qz	406	3	were	be	VBD
cord-268139-tgpsu4qz	406	4	then	then	RB
cord-268139-tgpsu4qz	406	5	subcloned	subclone	VBN
cord-268139-tgpsu4qz	406	6	into	into	IN
cord-268139-tgpsu4qz	406	7	pCR	pCR	NNP
cord-268139-tgpsu4qz	406	8	-	-	HYPH
cord-268139-tgpsu4qz	406	9	XL	XL	NNP
cord-268139-tgpsu4qz	406	10	-	-	HYPH
cord-268139-tgpsu4qz	406	11	TopoA	TopoA	NNP
cord-268139-tgpsu4qz	406	12	in	in	IN
cord-268139-tgpsu4qz	406	13	place	place	NN
cord-268139-tgpsu4qz	406	14	of	of	IN
cord-268139-tgpsu4qz	406	15	wild	wild	JJ
cord-268139-tgpsu4qz	406	16	-	-	HYPH
cord-268139-tgpsu4qz	406	17	type	type	NN
cord-268139-tgpsu4qz	406	18	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	406	19	using	use	VBG
cord-268139-tgpsu4qz	406	20	primer	primer	NN
cord-268139-tgpsu4qz	406	21	-	-	HYPH
cord-268139-tgpsu4qz	406	22	generated	generate	VBN
cord-268139-tgpsu4qz	406	23	restriction	restriction	NN
cord-268139-tgpsu4qz	406	24	sites	site	NNS
cord-268139-tgpsu4qz	406	25	:	:	:
cord-268139-tgpsu4qz	406	26	nsp1DFL	nsp1dfl	ADD
cord-268139-tgpsu4qz	406	27	(	(	-LRB-
cord-268139-tgpsu4qz	406	28	5VPmeI	5vpmei	CD
cord-268139-tgpsu4qz	406	29	to	to	IN
cord-268139-tgpsu4qz	406	30	3VAflII	3vaflii	CD
cord-268139-tgpsu4qz	406	31	)	)	-RRB-
cord-268139-tgpsu4qz	406	32	,	,	,
cord-268139-tgpsu4qz	406	33	nsp1DMid	nsp1dmid	FW
cord-268139-tgpsu4qz	406	34	(	(	-LRB-
cord-268139-tgpsu4qz	406	35	first	first	RB
cord-268139-tgpsu4qz	406	36	ligated	ligate	VBN
cord-268139-tgpsu4qz	406	37	at	at	IN
cord-268139-tgpsu4qz	406	38	the	the	DT
cord-268139-tgpsu4qz	406	39	BamHI	BamHI	NNP
cord-268139-tgpsu4qz	406	40	site	site	NN
cord-268139-tgpsu4qz	406	41	then	then	RB
cord-268139-tgpsu4qz	406	42	subcloned	subclone	VBD
cord-268139-tgpsu4qz	406	43	into	into	IN
cord-268139-tgpsu4qz	406	44	pCR	pCR	NNP
cord-268139-tgpsu4qz	406	45	-	-	HYPH
cord-268139-tgpsu4qz	406	46	XL	XL	NNP
cord-268139-tgpsu4qz	406	47	-	-	HYPH
cord-268139-tgpsu4qz	406	48	TopoA	TopoA	NNP
cord-268139-tgpsu4qz	406	49	using	use	VBG
cord-268139-tgpsu4qz	406	50	5V	5v	JJ
cord-268139-tgpsu4qz	406	51	SacII	SacII	NNP
cord-268139-tgpsu4qz	406	52	and	and	CC
cord-268139-tgpsu4qz	406	53	3VAflII	3vaflii	CD
cord-268139-tgpsu4qz	406	54	)	)	-RRB-
cord-268139-tgpsu4qz	406	55	,	,	,
cord-268139-tgpsu4qz	406	56	and	and	CC
cord-268139-tgpsu4qz	406	57	nsp1DC	nsp1DC	NNP
cord-268139-tgpsu4qz	406	58	(	(	-LRB-
cord-268139-tgpsu4qz	406	59	5V	5v	VB
cord-268139-tgpsu4qz	406	60	SacII	SacII	NNP
cord-268139-tgpsu4qz	406	61	to	to	IN
cord-268139-tgpsu4qz	406	62	3VAflII	3vaflii	CD
cord-268139-tgpsu4qz	406	63	)	)	-RRB-
cord-268139-tgpsu4qz	406	64	.	.	.
cord-268139-tgpsu4qz	407	1	All	all	DT
cord-268139-tgpsu4qz	407	2	fragment	fragment	NN
cord-268139-tgpsu4qz	407	3	A	a	DT
cord-268139-tgpsu4qz	407	4	plasmids	plasmid	NNS
cord-268139-tgpsu4qz	407	5	were	be	VBD
cord-268139-tgpsu4qz	407	6	sequenced	sequence	VBN
cord-268139-tgpsu4qz	407	7	across	across	IN
cord-268139-tgpsu4qz	407	8	the	the	DT
cord-268139-tgpsu4qz	407	9	nsp1coding	nsp1coding	NNP
cord-268139-tgpsu4qz	407	10	region	region	NN
cord-268139-tgpsu4qz	407	11	to	to	TO
cord-268139-tgpsu4qz	407	12	ensure	ensure	VB
cord-268139-tgpsu4qz	407	13	proper	proper	JJ
cord-268139-tgpsu4qz	407	14	ligation	ligation	NN
cord-268139-tgpsu4qz	407	15	and	and	CC
cord-268139-tgpsu4qz	407	16	maintenance	maintenance	NN
cord-268139-tgpsu4qz	407	17	of	of	IN
cord-268139-tgpsu4qz	407	18	the	the	DT
cord-268139-tgpsu4qz	407	19	translational	translational	JJ
cord-268139-tgpsu4qz	407	20	reading	reading	NN
cord-268139-tgpsu4qz	407	21	frame	frame	NN
cord-268139-tgpsu4qz	407	22	.	.	.
cord-268139-tgpsu4qz	408	1	The	the	DT
cord-268139-tgpsu4qz	408	2	MHV	MHV	NNP
cord-268139-tgpsu4qz	408	3	-	-	HYPH
cord-268139-tgpsu4qz	408	4	A59	A59	NNP
cord-268139-tgpsu4qz	408	5	nucleotides	nucleotide	NNS
cord-268139-tgpsu4qz	408	6	(	(	-LRB-
cord-268139-tgpsu4qz	408	7	nt	nt	RB
cord-268139-tgpsu4qz	408	8	)	)	-RRB-
cord-268139-tgpsu4qz	408	9	103	103	CD
cord-268139-tgpsu4qz	408	10	to	to	IN
cord-268139-tgpsu4qz	408	11	954	954	CD
cord-268139-tgpsu4qz	408	12	(	(	-LRB-
cord-268139-tgpsu4qz	408	13	restriction	restriction	NN
cord-268139-tgpsu4qz	408	14	sites	site	NNS
cord-268139-tgpsu4qz	408	15	5V	5v	VBP
cord-268139-tgpsu4qz	408	16	SacII	sacii	NN
cord-268139-tgpsu4qz	408	17	to	to	IN
cord-268139-tgpsu4qz	408	18	3VAflII	3vaflii	CD
cord-268139-tgpsu4qz	408	19	)	)	-RRB-
cord-268139-tgpsu4qz	408	20	were	be	VBD
cord-268139-tgpsu4qz	408	21	PCR	PCR	NNP
cord-268139-tgpsu4qz	408	22	amplified	amplify	VBN
cord-268139-tgpsu4qz	408	23	using	use	VBG
cord-268139-tgpsu4qz	408	24	pCR	pCR	NNP
cord-268139-tgpsu4qz	408	25	-	-	HYPH
cord-268139-tgpsu4qz	408	26	XL	XL	NNP
cord-268139-tgpsu4qz	408	27	-	-	HYPH
cord-268139-tgpsu4qz	408	28	TopoA	TopoA	NNP
cord-268139-tgpsu4qz	408	29	as	as	IN
cord-268139-tgpsu4qz	408	30	template	template	NN
cord-268139-tgpsu4qz	408	31	and	and	CC
cord-268139-tgpsu4qz	408	32	cloned	clone	VBN
cord-268139-tgpsu4qz	408	33	into	into	IN
cord-268139-tgpsu4qz	408	34	pGEM	pgem	JJ
cord-268139-tgpsu4qz	408	35	-	-	HYPH
cord-268139-tgpsu4qz	408	36	T	T	NNP
cord-268139-tgpsu4qz	408	37	-	-	HYPH
cord-268139-tgpsu4qz	408	38	Easy	Easy	NNP
cord-268139-tgpsu4qz	408	39	to	to	TO
cord-268139-tgpsu4qz	408	40	generate	generate	VB
cord-268139-tgpsu4qz	408	41	pGEM	pGEM	NNS
cord-268139-tgpsu4qz	408	42	-	-	HYPH
cord-268139-tgpsu4qz	408	43	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	408	44	.	.	.
cord-268139-tgpsu4qz	409	1	Charge	charge	NN
cord-268139-tgpsu4qz	409	2	-	-	HYPH
cord-268139-tgpsu4qz	409	3	to	to	IN
cord-268139-tgpsu4qz	409	4	-	-	HYPH
cord-268139-tgpsu4qz	409	5	alanine	alanine	NN
cord-268139-tgpsu4qz	409	6	substitutions	substitution	NNS
cord-268139-tgpsu4qz	409	7	were	be	VBD
cord-268139-tgpsu4qz	409	8	introduced	introduce	VBN
cord-268139-tgpsu4qz	409	9	into	into	IN
cord-268139-tgpsu4qz	409	10	pGEM	pGEM	NNS
cord-268139-tgpsu4qz	409	11	-	-	HYPH
cord-268139-tgpsu4qz	409	12	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	409	13	using	use	VBG
cord-268139-tgpsu4qz	409	14	complementary	complementary	JJ
cord-268139-tgpsu4qz	409	15	pairs	pair	NNS
cord-268139-tgpsu4qz	409	16	of	of	IN
cord-268139-tgpsu4qz	409	17	mutagenic	mutagenic	JJ
cord-268139-tgpsu4qz	409	18	primers	primer	NNS
cord-268139-tgpsu4qz	409	19	and	and	CC
cord-268139-tgpsu4qz	409	20	the	the	DT
cord-268139-tgpsu4qz	409	21	QuikChange	QuikChange	NNP
cord-268139-tgpsu4qz	409	22	Site	Site	NNP
cord-268139-tgpsu4qz	409	23	-	-	:
cord-268139-tgpsu4qz	409	24	Directed	Directed	NNP
cord-268139-tgpsu4qz	409	25	Mutagenesis	Mutagenesis	NNP
cord-268139-tgpsu4qz	409	26	Kit	kit	NN
cord-268139-tgpsu4qz	409	27	(	(	-LRB-
cord-268139-tgpsu4qz	409	28	Stratagene	Stratagene	NNP
cord-268139-tgpsu4qz	409	29	)	)	-RRB-
cord-268139-tgpsu4qz	409	30	.	.	.
cord-268139-tgpsu4qz	410	1	Mutagenized	Mutagenized	NNP
cord-268139-tgpsu4qz	411	1	pGEM	pGEM	NNP
cord-268139-tgpsu4qz	411	2	-	-	HYPH
cord-268139-tgpsu4qz	411	3	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	411	4	plasmids	plasmid	NNS
cord-268139-tgpsu4qz	411	5	were	be	VBD
cord-268139-tgpsu4qz	411	6	sequenced	sequence	VBN
cord-268139-tgpsu4qz	411	7	to	to	TO
cord-268139-tgpsu4qz	411	8	verify	verify	VB
cord-268139-tgpsu4qz	411	9	the	the	DT
cord-268139-tgpsu4qz	411	10	incorporation	incorporation	NN
cord-268139-tgpsu4qz	411	11	of	of	IN
cord-268139-tgpsu4qz	411	12	the	the	DT
cord-268139-tgpsu4qz	411	13	appropriate	appropriate	JJ
cord-268139-tgpsu4qz	411	14	substitutions	substitution	NNS
cord-268139-tgpsu4qz	411	15	and	and	CC
cord-268139-tgpsu4qz	411	16	the	the	DT
cord-268139-tgpsu4qz	411	17	fidelity	fidelity	NN
cord-268139-tgpsu4qz	411	18	of	of	IN
cord-268139-tgpsu4qz	411	19	PCR	PCR	NNP
cord-268139-tgpsu4qz	411	20	.	.	.
cord-268139-tgpsu4qz	412	1	Restriction	restriction	NN
cord-268139-tgpsu4qz	412	2	enzyme	enzyme	NN
cord-268139-tgpsu4qz	412	3	sites	site	NNS
cord-268139-tgpsu4qz	412	4	5V	5v	VBP
cord-268139-tgpsu4qz	412	5	SacII	SacII	NNP
cord-268139-tgpsu4qz	412	6	and	and	CC
cord-268139-tgpsu4qz	412	7	3VAflII	3vaflii	CD
cord-268139-tgpsu4qz	412	8	were	be	VBD
cord-268139-tgpsu4qz	412	9	then	then	RB
cord-268139-tgpsu4qz	412	10	used	use	VBN
cord-268139-tgpsu4qz	412	11	to	to	TO
cord-268139-tgpsu4qz	412	12	subclone	subclone	VB
cord-268139-tgpsu4qz	412	13	mutant	mutant	JJ
cord-268139-tgpsu4qz	412	14	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	412	15	sequences	sequence	NNS
cord-268139-tgpsu4qz	412	16	into	into	IN
cord-268139-tgpsu4qz	412	17	pCR	pCR	NNP
cord-268139-tgpsu4qz	412	18	-	-	HYPH
cord-268139-tgpsu4qz	412	19	XL	XL	NNP
cord-268139-tgpsu4qz	412	20	-	-	HYPH
cord-268139-tgpsu4qz	412	21	TopoA	TopoA	NNP
cord-268139-tgpsu4qz	412	22	in	in	IN
cord-268139-tgpsu4qz	412	23	place	place	NN
cord-268139-tgpsu4qz	412	24	of	of	IN
cord-268139-tgpsu4qz	412	25	the	the	DT
cord-268139-tgpsu4qz	412	26	wild	wild	JJ
cord-268139-tgpsu4qz	412	27	-	-	HYPH
cord-268139-tgpsu4qz	412	28	type	type	NN
cord-268139-tgpsu4qz	412	29	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	412	30	sequence	sequence	NN
cord-268139-tgpsu4qz	412	31	.	.	.
cord-268139-tgpsu4qz	413	1	Viruses	virus	NNS
cord-268139-tgpsu4qz	413	2	containing	contain	VBG
cord-268139-tgpsu4qz	413	3	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	413	4	mutations	mutation	NNS
cord-268139-tgpsu4qz	413	5	were	be	VBD
cord-268139-tgpsu4qz	413	6	produced	produce	VBN
cord-268139-tgpsu4qz	413	7	using	use	VBG
cord-268139-tgpsu4qz	413	8	the	the	DT
cord-268139-tgpsu4qz	413	9	infectious	infectious	JJ
cord-268139-tgpsu4qz	413	10	clone	clone	NN
cord-268139-tgpsu4qz	413	11	strategy	strategy	NN
cord-268139-tgpsu4qz	413	12	for	for	IN
cord-268139-tgpsu4qz	413	13	MHV	MHV	NNP
cord-268139-tgpsu4qz	413	14	-	-	HYPH
cord-268139-tgpsu4qz	413	15	A59	A59	NNP
cord-268139-tgpsu4qz	413	16	(	(	-LRB-
cord-268139-tgpsu4qz	413	17	icMHV	icmhv	NN
cord-268139-tgpsu4qz	413	18	)	)	-RRB-
cord-268139-tgpsu4qz	413	19	described	describe	VBN
cord-268139-tgpsu4qz	413	20	by	by	IN
cord-268139-tgpsu4qz	413	21	Yount	Yount	NNP
cord-268139-tgpsu4qz	413	22	et	et	NNP
cord-268139-tgpsu4qz	413	23	al	al	NNP
cord-268139-tgpsu4qz	413	24	.	.	.
cord-268139-tgpsu4qz	414	1	(	(	-LRB-
cord-268139-tgpsu4qz	414	2	2002	2002	CD
cord-268139-tgpsu4qz	414	3	)	)	-RRB-
cord-268139-tgpsu4qz	414	4	and	and	CC
cord-268139-tgpsu4qz	414	5	modified	modify	VBN
cord-268139-tgpsu4qz	414	6	by	by	IN
cord-268139-tgpsu4qz	414	7	Denison	Denison	NNP
cord-268139-tgpsu4qz	414	8	et	et	NNP
cord-268139-tgpsu4qz	414	9	al	al	NNP
cord-268139-tgpsu4qz	414	10	.	.	.
cord-268139-tgpsu4qz	415	1	(	(	-LRB-
cord-268139-tgpsu4qz	415	2	2004	2004	CD
cord-268139-tgpsu4qz	415	3	)	)	-RRB-
cord-268139-tgpsu4qz	415	4	.	.	.
cord-268139-tgpsu4qz	416	1	All	all	DT
cord-268139-tgpsu4qz	416	2	assembled	assemble	VBN
cord-268139-tgpsu4qz	416	3	viruses	virus	NNS
cord-268139-tgpsu4qz	416	4	,	,	,
cord-268139-tgpsu4qz	416	5	including	include	VBG
cord-268139-tgpsu4qz	416	6	the	the	DT
cord-268139-tgpsu4qz	416	7	wildtype	wildtype	NN
cord-268139-tgpsu4qz	416	8	control	control	NN
cord-268139-tgpsu4qz	416	9	(	(	-LRB-
cord-268139-tgpsu4qz	416	10	icwt	icwt	NNP
cord-268139-tgpsu4qz	416	11	)	)	-RRB-
cord-268139-tgpsu4qz	416	12	,	,	,
cord-268139-tgpsu4qz	416	13	were	be	VBD
cord-268139-tgpsu4qz	416	14	generated	generate	VBN
cord-268139-tgpsu4qz	416	15	using	use	VBG
cord-268139-tgpsu4qz	416	16	the	the	DT
cord-268139-tgpsu4qz	416	17	icMHV	icmhv	NN
cord-268139-tgpsu4qz	416	18	fragment	fragment	NN
cord-268139-tgpsu4qz	416	19	F	F	NNP
cord-268139-tgpsu4qz	416	20	plasmid	plasmid	NN
cord-268139-tgpsu4qz	416	21	corresponding	correspond	VBG
cord-268139-tgpsu4qz	416	22	to	to	IN
cord-268139-tgpsu4qz	416	23	the	the	DT
cord-268139-tgpsu4qz	416	24	virulent	virulent	JJ
cord-268139-tgpsu4qz	416	25	VUSS3	vuss3	NN
cord-268139-tgpsu4qz	416	26	strain	strain	NN
cord-268139-tgpsu4qz	416	27	(	(	-LRB-
cord-268139-tgpsu4qz	416	28	Sperry	Sperry	NNP
cord-268139-tgpsu4qz	416	29	et	et	NNP
cord-268139-tgpsu4qz	416	30	al	al	NNP
cord-268139-tgpsu4qz	416	31	.	.	NNP
cord-268139-tgpsu4qz	416	32	,	,	,
cord-268139-tgpsu4qz	416	33	2005	2005	CD
cord-268139-tgpsu4qz	416	34	)	)	-RRB-
cord-268139-tgpsu4qz	416	35	.	.	.
cord-268139-tgpsu4qz	417	1	Plasmids	plasmid	NNS
cord-268139-tgpsu4qz	417	2	containing	contain	VBG
cord-268139-tgpsu4qz	417	3	the	the	DT
cord-268139-tgpsu4qz	417	4	cDNA	cdna	NN
cord-268139-tgpsu4qz	417	5	cassettes	cassette	NNS
cord-268139-tgpsu4qz	417	6	of	of	IN
cord-268139-tgpsu4qz	417	7	the	the	DT
cord-268139-tgpsu4qz	417	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	417	9	genome	genome	NN
cord-268139-tgpsu4qz	417	10	were	be	VBD
cord-268139-tgpsu4qz	417	11	digested	digest	VBN
cord-268139-tgpsu4qz	417	12	using	use	VBG
cord-268139-tgpsu4qz	417	13	MluI	MluI	NNP
cord-268139-tgpsu4qz	417	14	and	and	CC
cord-268139-tgpsu4qz	417	15	BsmBI	BsmBI	NNP
cord-268139-tgpsu4qz	417	16	for	for	IN
cord-268139-tgpsu4qz	417	17	fragment	fragment	NN
cord-268139-tgpsu4qz	417	18	A	a	NN
cord-268139-tgpsu4qz	417	19	,	,	,
cord-268139-tgpsu4qz	417	20	BglI	BglI	NNP
cord-268139-tgpsu4qz	417	21	and	and	CC
cord-268139-tgpsu4qz	417	22	BsmBI	BsmBI	NNP
cord-268139-tgpsu4qz	417	23	for	for	IN
cord-268139-tgpsu4qz	417	24	fragments	fragment	NNS
cord-268139-tgpsu4qz	417	25	B	b	NN
cord-268139-tgpsu4qz	417	26	and	and	CC
cord-268139-tgpsu4qz	417	27	C	C	NNP
cord-268139-tgpsu4qz	417	28	,	,	,
cord-268139-tgpsu4qz	417	29	NciI	NciI	NNP
cord-268139-tgpsu4qz	417	30	and	and	CC
cord-268139-tgpsu4qz	417	31	BsmBI	BsmBI	NNP
cord-268139-tgpsu4qz	417	32	for	for	IN
cord-268139-tgpsu4qz	417	33	fragments	fragment	NNS
cord-268139-tgpsu4qz	417	34	D	d	NN
cord-268139-tgpsu4qz	417	35	and	and	CC
cord-268139-tgpsu4qz	417	36	E	e	NN
cord-268139-tgpsu4qz	417	37	,	,	,
cord-268139-tgpsu4qz	417	38	BsmBI	BsmBI	NNP
cord-268139-tgpsu4qz	417	39	for	for	IN
cord-268139-tgpsu4qz	417	40	F	F	NNP
cord-268139-tgpsu4qz	417	41	,	,	,
cord-268139-tgpsu4qz	417	42	and	and	CC
cord-268139-tgpsu4qz	417	43	SfiI	SfiI	NNP
cord-268139-tgpsu4qz	417	44	and	and	CC
cord-268139-tgpsu4qz	417	45	BsmBI	BsmBI	NNP
cord-268139-tgpsu4qz	418	1	for	for	IN
cord-268139-tgpsu4qz	418	2	fragment	fragment	NN
cord-268139-tgpsu4qz	418	3	G.	G.	NNP
cord-268139-tgpsu4qz	418	4	Gel	Gel	NNP
cord-268139-tgpsu4qz	418	5	purified	purify	VBN
cord-268139-tgpsu4qz	418	6	restriction	restriction	NN
cord-268139-tgpsu4qz	418	7	fragments	fragment	NNS
cord-268139-tgpsu4qz	418	8	were	be	VBD
cord-268139-tgpsu4qz	418	9	ligated	ligate	VBN
cord-268139-tgpsu4qz	418	10	together	together	RB
cord-268139-tgpsu4qz	418	11	using	use	VBG
cord-268139-tgpsu4qz	418	12	T4	T4	NNP
cord-268139-tgpsu4qz	418	13	DNA	DNA	NNP
cord-268139-tgpsu4qz	418	14	ligase	ligase	NN
cord-268139-tgpsu4qz	418	15	(	(	-LRB-
cord-268139-tgpsu4qz	418	16	New	New	NNP
cord-268139-tgpsu4qz	418	17	England	England	NNP
cord-268139-tgpsu4qz	418	18	Biolabs	Biolabs	NNP
cord-268139-tgpsu4qz	418	19	)	)	-RRB-
cord-268139-tgpsu4qz	418	20	in	in	IN
cord-268139-tgpsu4qz	418	21	a	a	DT
cord-268139-tgpsu4qz	418	22	total	total	JJ
cord-268139-tgpsu4qz	418	23	reaction	reaction	NN
cord-268139-tgpsu4qz	418	24	volume	volume	NN
cord-268139-tgpsu4qz	418	25	of	of	IN
cord-268139-tgpsu4qz	418	26	150	150	CD
cord-268139-tgpsu4qz	418	27	Al	Al	NNP
cord-268139-tgpsu4qz	418	28	at	at	IN
cord-268139-tgpsu4qz	418	29	16	16	CD
cord-268139-tgpsu4qz	418	30	-C	-C	NNS
cord-268139-tgpsu4qz	418	31	overnight	overnight	RB
cord-268139-tgpsu4qz	418	32	.	.	.
cord-268139-tgpsu4qz	419	1	Following	follow	VBG
cord-268139-tgpsu4qz	419	2	chloroform	chloroform	NN
cord-268139-tgpsu4qz	419	3	extraction	extraction	NN
cord-268139-tgpsu4qz	419	4	and	and	CC
cord-268139-tgpsu4qz	419	5	isopropanol	isopropanol	NN
cord-268139-tgpsu4qz	419	6	precipitation	precipitation	NN
cord-268139-tgpsu4qz	419	7	of	of	IN
cord-268139-tgpsu4qz	419	8	ligated	ligate	VBN
cord-268139-tgpsu4qz	419	9	cDNA	cdna	NN
cord-268139-tgpsu4qz	419	10	,	,	,
cord-268139-tgpsu4qz	419	11	full	full	JJ
cord-268139-tgpsu4qz	419	12	-	-	HYPH
cord-268139-tgpsu4qz	419	13	length	length	NN
cord-268139-tgpsu4qz	419	14	transcripts	transcript	NNS
cord-268139-tgpsu4qz	419	15	of	of	IN
cord-268139-tgpsu4qz	419	16	icMHV	icmhv	NN
cord-268139-tgpsu4qz	420	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	420	2	were	be	VBD
cord-268139-tgpsu4qz	420	3	generated	generate	VBN
cord-268139-tgpsu4qz	420	4	in	in	IN
cord-268139-tgpsu4qz	420	5	vitro	vitro	FW
cord-268139-tgpsu4qz	420	6	using	use	VBG
cord-268139-tgpsu4qz	420	7	the	the	DT
cord-268139-tgpsu4qz	420	8	mMessage	mMessage	NNP
cord-268139-tgpsu4qz	420	9	mMachine	mMachine	NNP
cord-268139-tgpsu4qz	420	10	T7	T7	NNP
cord-268139-tgpsu4qz	420	11	Transcription	Transcription	NNP
cord-268139-tgpsu4qz	420	12	Kit	kit	NN
cord-268139-tgpsu4qz	420	13	(	(	-LRB-
cord-268139-tgpsu4qz	420	14	Ambion	Ambion	NNP
cord-268139-tgpsu4qz	420	15	)	)	-RRB-
cord-268139-tgpsu4qz	420	16	according	accord	VBG
cord-268139-tgpsu4qz	420	17	to	to	IN
cord-268139-tgpsu4qz	420	18	the	the	DT
cord-268139-tgpsu4qz	420	19	manufacturer	manufacturer	NN
cord-268139-tgpsu4qz	420	20	's	's	POS
cord-268139-tgpsu4qz	420	21	protocol	protocol	NN
cord-268139-tgpsu4qz	420	22	with	with	IN
cord-268139-tgpsu4qz	420	23	the	the	DT
cord-268139-tgpsu4qz	420	24	following	following	JJ
cord-268139-tgpsu4qz	420	25	modifications	modification	NNS
cord-268139-tgpsu4qz	420	26	.	.	.
cord-268139-tgpsu4qz	421	1	Fifty	fifty	CD
cord-268139-tgpsu4qz	421	2	microliter	microliter	NN
cord-268139-tgpsu4qz	421	3	reactions	reaction	NNS
cord-268139-tgpsu4qz	421	4	were	be	VBD
cord-268139-tgpsu4qz	421	5	supplemented	supplement	VBN
cord-268139-tgpsu4qz	421	6	with	with	IN
cord-268139-tgpsu4qz	421	7	7.5	7.5	CD
cord-268139-tgpsu4qz	421	8	Al	Al	NNP
cord-268139-tgpsu4qz	421	9	of	of	IN
cord-268139-tgpsu4qz	421	10	30	30	CD
cord-268139-tgpsu4qz	421	11	mM	mm	CD
cord-268139-tgpsu4qz	421	12	GTP	GTP	NNP
cord-268139-tgpsu4qz	421	13	,	,	,
cord-268139-tgpsu4qz	421	14	and	and	CC
cord-268139-tgpsu4qz	421	15	transcription	transcription	NN
cord-268139-tgpsu4qz	421	16	was	be	VBD
cord-268139-tgpsu4qz	421	17	performed	perform	VBN
cord-268139-tgpsu4qz	421	18	at	at	IN
cord-268139-tgpsu4qz	421	19	40.5	40.5	CD
cord-268139-tgpsu4qz	421	20	-C	-C	:
cord-268139-tgpsu4qz	421	21	for	for	IN
cord-268139-tgpsu4qz	421	22	25	25	CD
cord-268139-tgpsu4qz	421	23	min	min	NN
cord-268139-tgpsu4qz	421	24	,	,	,
cord-268139-tgpsu4qz	421	25	37.5	37.5	CD
cord-268139-tgpsu4qz	421	26	-C	-C	:
cord-268139-tgpsu4qz	421	27	for	for	IN
cord-268139-tgpsu4qz	421	28	50	50	CD
cord-268139-tgpsu4qz	421	29	min	min	NN
cord-268139-tgpsu4qz	421	30	,	,	,
cord-268139-tgpsu4qz	421	31	and	and	CC
cord-268139-tgpsu4qz	421	32	40.5	40.5	CD
cord-268139-tgpsu4qz	421	33	-C	-C	NNS
cord-268139-tgpsu4qz	421	34	for	for	IN
cord-268139-tgpsu4qz	421	35	25	25	CD
cord-268139-tgpsu4qz	421	36	min	min	NN
cord-268139-tgpsu4qz	421	37	.	.	.
cord-268139-tgpsu4qz	422	1	In	in	IN
cord-268139-tgpsu4qz	422	2	parallel	parallel	JJ
cord-268139-tgpsu4qz	422	3	,	,	,
cord-268139-tgpsu4qz	422	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	422	5	transcripts	transcript	NNS
cord-268139-tgpsu4qz	422	6	encoding	encode	VBG
cord-268139-tgpsu4qz	422	7	the	the	DT
cord-268139-tgpsu4qz	422	8	MHV	MHV	NNP
cord-268139-tgpsu4qz	422	9	nucleocapsid	nucleocapsid	NN
cord-268139-tgpsu4qz	422	10	protein	protein	NN
cord-268139-tgpsu4qz	422	11	(	(	-LRB-
cord-268139-tgpsu4qz	422	12	N	N	NNP
cord-268139-tgpsu4qz	422	13	)	)	-RRB-
cord-268139-tgpsu4qz	422	14	were	be	VBD
cord-268139-tgpsu4qz	422	15	generated	generate	VBN
cord-268139-tgpsu4qz	422	16	from	from	IN
cord-268139-tgpsu4qz	422	17	N	n	CD
cord-268139-tgpsu4qz	422	18	cDNA	cdna	NN
cord-268139-tgpsu4qz	422	19	.	.	.
cord-268139-tgpsu4qz	423	1	N	n	CD
cord-268139-tgpsu4qz	423	2	transcripts	transcript	NNS
cord-268139-tgpsu4qz	423	3	and	and	CC
cord-268139-tgpsu4qz	423	4	icMHV	icmhv	NN
cord-268139-tgpsu4qz	424	1	genomic	genomic	JJ
cord-268139-tgpsu4qz	424	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	424	3	were	be	VBD
cord-268139-tgpsu4qz	424	4	then	then	RB
cord-268139-tgpsu4qz	424	5	mixed	mix	VBN
cord-268139-tgpsu4qz	424	6	and	and	CC
cord-268139-tgpsu4qz	424	7	electroporated	electroporate	VBN
cord-268139-tgpsu4qz	424	8	into	into	IN
cord-268139-tgpsu4qz	424	9	BHK	BHK	NNP
cord-268139-tgpsu4qz	424	10	-	-	HYPH
cord-268139-tgpsu4qz	424	11	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	424	12	cells	cell	NNS
cord-268139-tgpsu4qz	424	13	.	.	.
cord-268139-tgpsu4qz	425	1	Briefly	briefly	RB
cord-268139-tgpsu4qz	425	2	,	,	,
cord-268139-tgpsu4qz	425	3	BHK	BHK	NNP
cord-268139-tgpsu4qz	425	4	-	-	HYPH
cord-268139-tgpsu4qz	425	5	MHVR	MHVR	NNP
cord-268139-tgpsu4qz	425	6	cells	cell	NNS
cord-268139-tgpsu4qz	425	7	were	be	VBD
cord-268139-tgpsu4qz	425	8	grown	grow	VBN
cord-268139-tgpsu4qz	425	9	to	to	IN
cord-268139-tgpsu4qz	425	10	sub	sub	JJ
cord-268139-tgpsu4qz	425	11	-	-	JJ
cord-268139-tgpsu4qz	425	12	confluence	confluence	JJ
cord-268139-tgpsu4qz	425	13	,	,	,
cord-268139-tgpsu4qz	425	14	trypsinized	trypsinize	VBN
cord-268139-tgpsu4qz	425	15	,	,	,
cord-268139-tgpsu4qz	425	16	then	then	RB
cord-268139-tgpsu4qz	425	17	washed	wash	VBN
cord-268139-tgpsu4qz	425	18	twice	twice	RB
cord-268139-tgpsu4qz	425	19	with	with	IN
cord-268139-tgpsu4qz	425	20	phosphate	phosphate	NN
cord-268139-tgpsu4qz	425	21	-	-	HYPH
cord-268139-tgpsu4qz	425	22	buffered	buffer	VBN
cord-268139-tgpsu4qz	425	23	saline	saline	NN
cord-268139-tgpsu4qz	425	24	(	(	-LRB-
cord-268139-tgpsu4qz	425	25	PBS	PBS	NNP
cord-268139-tgpsu4qz	425	26	)	)	-RRB-
cord-268139-tgpsu4qz	425	27	and	and	CC
cord-268139-tgpsu4qz	425	28	resuspended	resuspend	VBD
cord-268139-tgpsu4qz	425	29	in	in	IN
cord-268139-tgpsu4qz	425	30	PBS	PBS	NNP
cord-268139-tgpsu4qz	425	31	at	at	IN
cord-268139-tgpsu4qz	425	32	10	10	CD
cord-268139-tgpsu4qz	425	33	7	7	CD
cord-268139-tgpsu4qz	425	34	cells	cell	NNS
cord-268139-tgpsu4qz	425	35	/	/	SYM
cord-268139-tgpsu4qz	425	36	ml	ml	NN
cord-268139-tgpsu4qz	425	37	.	.	.
cord-268139-tgpsu4qz	426	1	Six	six	CD
cord-268139-tgpsu4qz	426	2	hundred	hundred	CD
cord-268139-tgpsu4qz	426	3	microliters	microliter	NNS
cord-268139-tgpsu4qz	426	4	cells	cell	NNS
cord-268139-tgpsu4qz	426	5	were	be	VBD
cord-268139-tgpsu4qz	426	6	then	then	RB
cord-268139-tgpsu4qz	426	7	added	add	VBN
cord-268139-tgpsu4qz	426	8	to	to	IN
cord-268139-tgpsu4qz	426	9	RNA	RNA	NNP
cord-268139-tgpsu4qz	426	10	transcripts	transcript	NNS
cord-268139-tgpsu4qz	426	11	in	in	IN
cord-268139-tgpsu4qz	426	12	a	a	DT
cord-268139-tgpsu4qz	426	13	4-mm	4-mm	CD
cord-268139-tgpsu4qz	426	14	gap	gap	NN
cord-268139-tgpsu4qz	426	15	electroporation	electroporation	NN
cord-268139-tgpsu4qz	426	16	cuvette	cuvette	NN
cord-268139-tgpsu4qz	426	17	,	,	,
cord-268139-tgpsu4qz	426	18	and	and	CC
cord-268139-tgpsu4qz	426	19	three	three	CD
cord-268139-tgpsu4qz	426	20	electrical	electrical	JJ
cord-268139-tgpsu4qz	426	21	pulses	pulse	NNS
cord-268139-tgpsu4qz	426	22	of	of	IN
cord-268139-tgpsu4qz	426	23	850	850	CD
cord-268139-tgpsu4qz	426	24	V	v	NN
cord-268139-tgpsu4qz	426	25	at	at	IN
cord-268139-tgpsu4qz	426	26	25	25	CD
cord-268139-tgpsu4qz	426	27	AF	AF	NNP
cord-268139-tgpsu4qz	426	28	were	be	VBD
cord-268139-tgpsu4qz	426	29	delivered	deliver	VBN
cord-268139-tgpsu4qz	426	30	with	with	IN
cord-268139-tgpsu4qz	426	31	a	a	DT
cord-268139-tgpsu4qz	426	32	Gene	Gene	NNP
cord-268139-tgpsu4qz	426	33	Pulser	Pulser	NNP
cord-268139-tgpsu4qz	426	34	II	II	NNP
cord-268139-tgpsu4qz	426	35	electroporator	electroporator	NNP
cord-268139-tgpsu4qz	426	36	(	(	-LRB-
cord-268139-tgpsu4qz	426	37	Bio	Bio	NNP
cord-268139-tgpsu4qz	426	38	-	-	HYPH
cord-268139-tgpsu4qz	426	39	Rad	Rad	NNP
cord-268139-tgpsu4qz	426	40	)	)	-RRB-
cord-268139-tgpsu4qz	426	41	.	.	.
cord-268139-tgpsu4qz	427	1	Transfected	transfected	JJ
cord-268139-tgpsu4qz	427	2	cells	cell	NNS
cord-268139-tgpsu4qz	427	3	were	be	VBD
cord-268139-tgpsu4qz	427	4	then	then	RB
cord-268139-tgpsu4qz	427	5	seeded	seed	VBN
cord-268139-tgpsu4qz	427	6	on	on	IN
cord-268139-tgpsu4qz	427	7	a	a	DT
cord-268139-tgpsu4qz	427	8	monolayer	monolayer	NN
cord-268139-tgpsu4qz	427	9	of	of	IN
cord-268139-tgpsu4qz	427	10	10	10	CD
cord-268139-tgpsu4qz	427	11	6	6	CD
cord-268139-tgpsu4qz	427	12	uninfected	uninfected	JJ
cord-268139-tgpsu4qz	427	13	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	427	14	cells	cell	NNS
cord-268139-tgpsu4qz	427	15	in	in	IN
cord-268139-tgpsu4qz	427	16	a	a	DT
cord-268139-tgpsu4qz	427	17	150	150	CD
cord-268139-tgpsu4qz	427	18	cm	cm	NN
cord-268139-tgpsu4qz	427	19	2	2	CD
cord-268139-tgpsu4qz	427	20	flask	flask	NN
cord-268139-tgpsu4qz	427	21	and	and	CC
cord-268139-tgpsu4qz	427	22	incubated	incubate	VBN
cord-268139-tgpsu4qz	427	23	at	at	IN
cord-268139-tgpsu4qz	427	24	either	either	DT
cord-268139-tgpsu4qz	427	25	37	37	CD
cord-268139-tgpsu4qz	427	26	-C	-C	NNS
cord-268139-tgpsu4qz	427	27	or	or	CC
cord-268139-tgpsu4qz	427	28	32	32	CD
cord-268139-tgpsu4qz	427	29	-C	-C	NNS
cord-268139-tgpsu4qz	427	30	for	for	IN
cord-268139-tgpsu4qz	427	31	24	24	CD
cord-268139-tgpsu4qz	427	32	to	to	TO
cord-268139-tgpsu4qz	427	33	96	96	CD
cord-268139-tgpsu4qz	427	34	h.	h.	NN
cord-268139-tgpsu4qz	427	35	Virus	virus	NN
cord-268139-tgpsu4qz	427	36	viability	viability	NN
cord-268139-tgpsu4qz	427	37	was	be	VBD
cord-268139-tgpsu4qz	427	38	determined	determine	VBN
cord-268139-tgpsu4qz	427	39	by	by	IN
cord-268139-tgpsu4qz	427	40	syncytia	syncytia	NN
cord-268139-tgpsu4qz	427	41	formation	formation	NN
cord-268139-tgpsu4qz	427	42	in	in	IN
cord-268139-tgpsu4qz	427	43	the	the	DT
cord-268139-tgpsu4qz	427	44	electroporated	electroporated	JJ
cord-268139-tgpsu4qz	427	45	cell	cell	NN
cord-268139-tgpsu4qz	427	46	culture	culture	NN
cord-268139-tgpsu4qz	427	47	and	and	CC
cord-268139-tgpsu4qz	427	48	by	by	IN
cord-268139-tgpsu4qz	427	49	the	the	DT
cord-268139-tgpsu4qz	427	50	capacity	capacity	NN
cord-268139-tgpsu4qz	427	51	of	of	IN
cord-268139-tgpsu4qz	427	52	the	the	DT
cord-268139-tgpsu4qz	427	53	clarified	clarify	VBN
cord-268139-tgpsu4qz	427	54	transfected	transfected	JJ
cord-268139-tgpsu4qz	427	55	cell	cell	NN
cord-268139-tgpsu4qz	427	56	supernatant	supernatant	VBN
cord-268139-tgpsu4qz	427	57	to	to	TO
cord-268139-tgpsu4qz	427	58	induce	induce	VB
cord-268139-tgpsu4qz	427	59	syncytia	syncytia	NN
cord-268139-tgpsu4qz	427	60	in	in	IN
cord-268139-tgpsu4qz	427	61	a	a	DT
cord-268139-tgpsu4qz	427	62	monolayer	monolayer	NN
cord-268139-tgpsu4qz	427	63	of	of	IN
cord-268139-tgpsu4qz	427	64	fresh	fresh	JJ
cord-268139-tgpsu4qz	427	65	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	427	66	cells	cell	NNS
cord-268139-tgpsu4qz	427	67	.	.	.
cord-268139-tgpsu4qz	428	1	Plaque	plaque	NN
cord-268139-tgpsu4qz	428	2	purification	purification	NN
cord-268139-tgpsu4qz	428	3	,	,	,
cord-268139-tgpsu4qz	428	4	RT	RT	NNP
cord-268139-tgpsu4qz	428	5	-	-	HYPH
cord-268139-tgpsu4qz	428	6	PCR	PCR	NNP
cord-268139-tgpsu4qz	428	7	,	,	,
cord-268139-tgpsu4qz	428	8	and	and	CC
cord-268139-tgpsu4qz	428	9	sequencing	sequence	VBG
cord-268139-tgpsu4qz	428	10	Mutant	mutant	JJ
cord-268139-tgpsu4qz	428	11	viruses	virus	NNS
cord-268139-tgpsu4qz	428	12	were	be	VBD
cord-268139-tgpsu4qz	428	13	subjected	subject	VBN
cord-268139-tgpsu4qz	428	14	to	to	IN
cord-268139-tgpsu4qz	428	15	three	three	CD
cord-268139-tgpsu4qz	428	16	rounds	round	NNS
cord-268139-tgpsu4qz	428	17	of	of	IN
cord-268139-tgpsu4qz	428	18	plaque	plaque	NN
cord-268139-tgpsu4qz	428	19	purification	purification	NN
cord-268139-tgpsu4qz	428	20	,	,	,
cord-268139-tgpsu4qz	428	21	and	and	CC
cord-268139-tgpsu4qz	428	22	reverse	reverse	VB
cord-268139-tgpsu4qz	428	23	transcription	transcription	NN
cord-268139-tgpsu4qz	428	24	(	(	-LRB-
cord-268139-tgpsu4qz	428	25	RT)-PCR	RT)-PCR	NNP
cord-268139-tgpsu4qz	428	26	was	be	VBD
cord-268139-tgpsu4qz	428	27	used	use	VBN
cord-268139-tgpsu4qz	428	28	to	to	TO
cord-268139-tgpsu4qz	428	29	amplify	amplify	VB
cord-268139-tgpsu4qz	428	30	the	the	DT
cord-268139-tgpsu4qz	428	31	5V	5v	JJ
cord-268139-tgpsu4qz	428	32	end	end	NN
cord-268139-tgpsu4qz	428	33	of	of	IN
cord-268139-tgpsu4qz	428	34	the	the	DT
cord-268139-tgpsu4qz	428	35	viral	viral	JJ
cord-268139-tgpsu4qz	428	36	genome	genome	NN
cord-268139-tgpsu4qz	428	37	for	for	IN
cord-268139-tgpsu4qz	428	38	sequence	sequence	NN
cord-268139-tgpsu4qz	428	39	analysis	analysis	NN
cord-268139-tgpsu4qz	428	40	.	.	.
cord-268139-tgpsu4qz	429	1	RNA	RNA	NNP
cord-268139-tgpsu4qz	429	2	was	be	VBD
cord-268139-tgpsu4qz	429	3	harvested	harvest	VBN
cord-268139-tgpsu4qz	429	4	from	from	IN
cord-268139-tgpsu4qz	429	5	infected	infected	JJ
cord-268139-tgpsu4qz	429	6	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	429	7	cells	cell	NNS
cord-268139-tgpsu4qz	429	8	using	use	VBG
cord-268139-tgpsu4qz	429	9	Trizol	Trizol	NNP
cord-268139-tgpsu4qz	429	10	(	(	-LRB-
cord-268139-tgpsu4qz	429	11	Invitrogen	Invitrogen	NNP
cord-268139-tgpsu4qz	429	12	)	)	-RRB-
cord-268139-tgpsu4qz	429	13	according	accord	VBG
cord-268139-tgpsu4qz	429	14	to	to	IN
cord-268139-tgpsu4qz	429	15	the	the	DT
cord-268139-tgpsu4qz	429	16	manufacturer	manufacturer	NN
cord-268139-tgpsu4qz	429	17	's	's	POS
cord-268139-tgpsu4qz	429	18	protocol	protocol	NN
cord-268139-tgpsu4qz	429	19	and	and	CC
cord-268139-tgpsu4qz	429	20	used	use	VBD
cord-268139-tgpsu4qz	429	21	as	as	IN
cord-268139-tgpsu4qz	429	22	template	template	NN
cord-268139-tgpsu4qz	429	23	for	for	IN
cord-268139-tgpsu4qz	429	24	RT	RT	NNP
cord-268139-tgpsu4qz	429	25	-	-	HYPH
cord-268139-tgpsu4qz	429	26	PCR	PCR	NNP
cord-268139-tgpsu4qz	429	27	.	.	.
cord-268139-tgpsu4qz	430	1	To	to	TO
cord-268139-tgpsu4qz	430	2	generate	generate	VB
cord-268139-tgpsu4qz	430	3	viral	viral	JJ
cord-268139-tgpsu4qz	430	4	cDNA	cdna	NN
cord-268139-tgpsu4qz	430	5	,	,	,
cord-268139-tgpsu4qz	430	6	reverse	reverse	JJ
cord-268139-tgpsu4qz	430	7	transcription	transcription	NN
cord-268139-tgpsu4qz	430	8	was	be	VBD
cord-268139-tgpsu4qz	430	9	performed	perform	VBN
cord-268139-tgpsu4qz	430	10	using	use	VBG
cord-268139-tgpsu4qz	430	11	Superscript	Superscript	NNP
cord-268139-tgpsu4qz	430	12	II	II	NNP
cord-268139-tgpsu4qz	430	13	RT	RT	NNP
cord-268139-tgpsu4qz	430	14	(	(	-LRB-
cord-268139-tgpsu4qz	430	15	Invitrogen	Invitrogen	NNP
cord-268139-tgpsu4qz	430	16	)	)	-RRB-
cord-268139-tgpsu4qz	430	17	and	and	CC
cord-268139-tgpsu4qz	430	18	an	an	DT
cord-268139-tgpsu4qz	430	19	antisense	antisense	NN
cord-268139-tgpsu4qz	430	20	primer	primer	NN
cord-268139-tgpsu4qz	430	21	complimentary	complimentary	JJ
cord-268139-tgpsu4qz	430	22	to	to	IN
cord-268139-tgpsu4qz	430	23	nt	nt	RB
cord-268139-tgpsu4qz	430	24	5531	5531	CD
cord-268139-tgpsu4qz	431	1	-5500	-5500	.
cord-268139-tgpsu4qz	432	1	of	of	IN
cord-268139-tgpsu4qz	432	2	the	the	DT
cord-268139-tgpsu4qz	432	3	MHV	MHV	NNP
cord-268139-tgpsu4qz	432	4	-	-	HYPH
cord-268139-tgpsu4qz	432	5	A59	A59	NNP
cord-268139-tgpsu4qz	432	6	genome	genome	NN
cord-268139-tgpsu4qz	432	7	.	.	.
cord-268139-tgpsu4qz	433	1	The	the	DT
cord-268139-tgpsu4qz	433	2	nsp1-coding	nsp1-coding	NNP
cord-268139-tgpsu4qz	433	3	region	region	NN
cord-268139-tgpsu4qz	433	4	was	be	VBD
cord-268139-tgpsu4qz	433	5	then	then	RB
cord-268139-tgpsu4qz	433	6	amplified	amplify	VBN
cord-268139-tgpsu4qz	433	7	using	use	VBG
cord-268139-tgpsu4qz	433	8	PCR	PCR	NNP
cord-268139-tgpsu4qz	433	9	with	with	IN
cord-268139-tgpsu4qz	433	10	primers	primer	NNS
cord-268139-tgpsu4qz	433	11	corresponding	correspond	VBG
cord-268139-tgpsu4qz	433	12	to	to	IN
cord-268139-tgpsu4qz	433	13	nt	nt	RB
cord-268139-tgpsu4qz	433	14	284	284	CD
cord-268139-tgpsu4qz	433	15	-	-	HYPH
cord-268139-tgpsu4qz	433	16	302	302	CD
cord-268139-tgpsu4qz	433	17	(	(	-LRB-
cord-268139-tgpsu4qz	433	18	sense	sense	NN
cord-268139-tgpsu4qz	433	19	)	)	-RRB-
cord-268139-tgpsu4qz	433	20	and	and	CC
cord-268139-tgpsu4qz	433	21	nt	nt	RB
cord-268139-tgpsu4qz	433	22	1150	1150	CD
cord-268139-tgpsu4qz	433	23	-	-	SYM
cord-268139-tgpsu4qz	433	24	1165	1165	CD
cord-268139-tgpsu4qz	433	25	(	(	-LRB-
cord-268139-tgpsu4qz	433	26	antisense	antisense	NN
cord-268139-tgpsu4qz	433	27	)	)	-RRB-
cord-268139-tgpsu4qz	433	28	.	.	.
cord-268139-tgpsu4qz	434	1	The	the	DT
cord-268139-tgpsu4qz	434	2	resulting	result	VBG
cord-268139-tgpsu4qz	434	3	amplicons	amplicon	NNS
cord-268139-tgpsu4qz	434	4	were	be	VBD
cord-268139-tgpsu4qz	434	5	sequenced	sequence	VBN
cord-268139-tgpsu4qz	434	6	across	across	IN
cord-268139-tgpsu4qz	434	7	the	the	DT
cord-268139-tgpsu4qz	434	8	nsp1-coding	nsp1-coding	NN
cord-268139-tgpsu4qz	434	9	region	region	NN
cord-268139-tgpsu4qz	434	10	to	to	TO
cord-268139-tgpsu4qz	434	11	confirm	confirm	VB
cord-268139-tgpsu4qz	434	12	the	the	DT
cord-268139-tgpsu4qz	434	13	retention	retention	NN
cord-268139-tgpsu4qz	434	14	of	of	IN
cord-268139-tgpsu4qz	434	15	the	the	DT
cord-268139-tgpsu4qz	434	16	introduced	introduce	VBN
cord-268139-tgpsu4qz	434	17	nsp1	nsp1	CD
cord-268139-tgpsu4qz	434	18	mutations	mutation	NNS
cord-268139-tgpsu4qz	434	19	and	and	CC
cord-268139-tgpsu4qz	434	20	the	the	DT
cord-268139-tgpsu4qz	434	21	absence	absence	NN
cord-268139-tgpsu4qz	434	22	of	of	IN
cord-268139-tgpsu4qz	434	23	second	second	JJ
cord-268139-tgpsu4qz	434	24	-	-	HYPH
cord-268139-tgpsu4qz	434	25	site	site	NN
cord-268139-tgpsu4qz	434	26	mutations	mutation	NNS
cord-268139-tgpsu4qz	434	27	.	.	.
cord-268139-tgpsu4qz	435	1	Viable	viable	JJ
cord-268139-tgpsu4qz	435	2	nsp1	nsp1	NNS
cord-268139-tgpsu4qz	435	3	mutant	mutant	JJ
cord-268139-tgpsu4qz	435	4	viruses	virus	NNS
cord-268139-tgpsu4qz	435	5	were	be	VBD
cord-268139-tgpsu4qz	435	6	analyzed	analyze	VBN
cord-268139-tgpsu4qz	435	7	for	for	IN
cord-268139-tgpsu4qz	435	8	replication	replication	NN
cord-268139-tgpsu4qz	435	9	using	use	VBG
cord-268139-tgpsu4qz	435	10	single	single	JJ
cord-268139-tgpsu4qz	435	11	-	-	HYPH
cord-268139-tgpsu4qz	435	12	cycle	cycle	NN
cord-268139-tgpsu4qz	435	13	replication	replication	NN
cord-268139-tgpsu4qz	435	14	assays	assay	NNS
cord-268139-tgpsu4qz	435	15	as	as	IN
cord-268139-tgpsu4qz	435	16	previously	previously	RB
cord-268139-tgpsu4qz	435	17	described	describe	VBN
cord-268139-tgpsu4qz	435	18	.	.	.
cord-268139-tgpsu4qz	436	1	Briefly	briefly	RB
cord-268139-tgpsu4qz	436	2	,	,	,
cord-268139-tgpsu4qz	436	3	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	436	4	cells	cell	NNS
cord-268139-tgpsu4qz	436	5	(	(	-LRB-
cord-268139-tgpsu4qz	436	6	approximately	approximately	RB
cord-268139-tgpsu4qz	436	7	10	10	CD
cord-268139-tgpsu4qz	436	8	6	6	CD
cord-268139-tgpsu4qz	436	9	cells	cell	NNS
cord-268139-tgpsu4qz	436	10	)	)	-RRB-
cord-268139-tgpsu4qz	436	11	were	be	VBD
cord-268139-tgpsu4qz	436	12	infected	infect	VBN
cord-268139-tgpsu4qz	436	13	at	at	IN
cord-268139-tgpsu4qz	436	14	an	an	DT
cord-268139-tgpsu4qz	436	15	MOI	MOI	NNP
cord-268139-tgpsu4qz	436	16	of	of	IN
cord-268139-tgpsu4qz	436	17	5	5	CD
cord-268139-tgpsu4qz	436	18	PFU	pfu	NN
cord-268139-tgpsu4qz	436	19	/	/	SYM
cord-268139-tgpsu4qz	436	20	cell	cell	NN
cord-268139-tgpsu4qz	436	21	with	with	IN
cord-268139-tgpsu4qz	436	22	icwt	icwt	NNP
cord-268139-tgpsu4qz	436	23	or	or	CC
cord-268139-tgpsu4qz	436	24	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	436	25	mutant	mutant	JJ
cord-268139-tgpsu4qz	436	26	viruses	virus	NNS
cord-268139-tgpsu4qz	436	27	.	.	.
cord-268139-tgpsu4qz	437	1	Virus	virus	NN
cord-268139-tgpsu4qz	437	2	inoculum	inoculum	NN
cord-268139-tgpsu4qz	437	3	was	be	VBD
cord-268139-tgpsu4qz	437	4	removed	remove	VBN
cord-268139-tgpsu4qz	437	5	following	follow	VBG
cord-268139-tgpsu4qz	437	6	30	30	CD
cord-268139-tgpsu4qz	437	7	min	min	NN
cord-268139-tgpsu4qz	437	8	adsorption	adsorption	NN
cord-268139-tgpsu4qz	437	9	at	at	IN
cord-268139-tgpsu4qz	437	10	25	25	CD
cord-268139-tgpsu4qz	437	11	-C	-C	NNS
cord-268139-tgpsu4qz	437	12	,	,	,
cord-268139-tgpsu4qz	437	13	and	and	CC
cord-268139-tgpsu4qz	437	14	cells	cell	NNS
cord-268139-tgpsu4qz	437	15	were	be	VBD
cord-268139-tgpsu4qz	437	16	washed	wash	VBN
cord-268139-tgpsu4qz	437	17	three	three	CD
cord-268139-tgpsu4qz	437	18	times	time	NNS
cord-268139-tgpsu4qz	437	19	with	with	IN
cord-268139-tgpsu4qz	437	20	PBS	PBS	NNP
cord-268139-tgpsu4qz	437	21	.	.	.
cord-268139-tgpsu4qz	438	1	Cells	cell	NNS
cord-268139-tgpsu4qz	438	2	were	be	VBD
cord-268139-tgpsu4qz	438	3	then	then	RB
cord-268139-tgpsu4qz	438	4	incubated	incubate	VBN
cord-268139-tgpsu4qz	438	5	at	at	IN
cord-268139-tgpsu4qz	438	6	37	37	CD
cord-268139-tgpsu4qz	438	7	-C	-C	NNS
cord-268139-tgpsu4qz	438	8	in	in	IN
cord-268139-tgpsu4qz	438	9	fresh	fresh	JJ
cord-268139-tgpsu4qz	438	10	pre	pre	JJ
cord-268139-tgpsu4qz	438	11	-	-	JJ
cord-268139-tgpsu4qz	438	12	warmed	warm	VBN
cord-268139-tgpsu4qz	438	13	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	438	14	with	with	IN
cord-268139-tgpsu4qz	438	15	10	10	CD
cord-268139-tgpsu4qz	438	16	%	%	NN
cord-268139-tgpsu4qz	438	17	FCS	FCS	NNP
cord-268139-tgpsu4qz	438	18	,	,	,
cord-268139-tgpsu4qz	438	19	and	and	CC
cord-268139-tgpsu4qz	438	20	samples	sample	NNS
cord-268139-tgpsu4qz	438	21	of	of	IN
cord-268139-tgpsu4qz	438	22	medium	medium	NN
cord-268139-tgpsu4qz	438	23	were	be	VBD
cord-268139-tgpsu4qz	438	24	collected	collect	VBN
cord-268139-tgpsu4qz	438	25	at	at	IN
cord-268139-tgpsu4qz	438	26	various	various	JJ
cord-268139-tgpsu4qz	438	27	times	time	NNS
cord-268139-tgpsu4qz	438	28	up	up	IN
cord-268139-tgpsu4qz	438	29	to	to	TO
cord-268139-tgpsu4qz	438	30	24	24	CD
cord-268139-tgpsu4qz	438	31	h	h	NN
cord-268139-tgpsu4qz	438	32	post	post	NN
cord-268139-tgpsu4qz	438	33	-	-	NN
cord-268139-tgpsu4qz	438	34	infection	infection	JJ
cord-268139-tgpsu4qz	438	35	(	(	-LRB-
cord-268139-tgpsu4qz	438	36	p.i	p.i	NNP
cord-268139-tgpsu4qz	438	37	.	.	NNP
cord-268139-tgpsu4qz	438	38	)	)	-RRB-
cord-268139-tgpsu4qz	438	39	.	.	.
cord-268139-tgpsu4qz	439	1	Viral	viral	JJ
cord-268139-tgpsu4qz	439	2	titers	titer	NNS
cord-268139-tgpsu4qz	439	3	in	in	IN
cord-268139-tgpsu4qz	439	4	the	the	DT
cord-268139-tgpsu4qz	439	5	medium	medium	NN
cord-268139-tgpsu4qz	439	6	were	be	VBD
cord-268139-tgpsu4qz	439	7	determined	determine	VBN
cord-268139-tgpsu4qz	439	8	by	by	IN
cord-268139-tgpsu4qz	439	9	plaque	plaque	NN
cord-268139-tgpsu4qz	439	10	assay	assay	NN
cord-268139-tgpsu4qz	439	11	(	(	-LRB-
cord-268139-tgpsu4qz	439	12	Hirano	Hirano	NNP
cord-268139-tgpsu4qz	439	13	et	et	FW
cord-268139-tgpsu4qz	439	14	al	al	NNP
cord-268139-tgpsu4qz	439	15	.	.	NNP
cord-268139-tgpsu4qz	439	16	,	,	,
cord-268139-tgpsu4qz	439	17	1976	1976	CD
cord-268139-tgpsu4qz	439	18	)	)	-RRB-
cord-268139-tgpsu4qz	439	19	.	.	.
cord-268139-tgpsu4qz	440	1	To	to	TO
cord-268139-tgpsu4qz	440	2	determine	determine	VB
cord-268139-tgpsu4qz	440	3	viral	viral	JJ
cord-268139-tgpsu4qz	440	4	yield	yield	NN
cord-268139-tgpsu4qz	440	5	,	,	,
cord-268139-tgpsu4qz	440	6	titer	titer	NN
cord-268139-tgpsu4qz	440	7	at	at	IN
cord-268139-tgpsu4qz	440	8	1	1	CD
cord-268139-tgpsu4qz	440	9	h	h	NN
cord-268139-tgpsu4qz	440	10	p.i	p.i	NNP
cord-268139-tgpsu4qz	440	11	.	.	NNP
cord-268139-tgpsu4qz	440	12	was	be	VBD
cord-268139-tgpsu4qz	440	13	subtracted	subtract	VBN
cord-268139-tgpsu4qz	440	14	from	from	IN
cord-268139-tgpsu4qz	440	15	peak	peak	NN
cord-268139-tgpsu4qz	440	16	titer	titer	NN
cord-268139-tgpsu4qz	440	17	for	for	IN
cord-268139-tgpsu4qz	440	18	each	each	DT
cord-268139-tgpsu4qz	440	19	virus	virus	NN
cord-268139-tgpsu4qz	440	20	,	,	,
cord-268139-tgpsu4qz	440	21	and	and	CC
cord-268139-tgpsu4qz	440	22	an	an	DT
cord-268139-tgpsu4qz	440	23	average	average	NN
cord-268139-tgpsu4qz	440	24	was	be	VBD
cord-268139-tgpsu4qz	440	25	calculated	calculate	VBN
cord-268139-tgpsu4qz	440	26	using	use	VBG
cord-268139-tgpsu4qz	440	27	data	datum	NNS
cord-268139-tgpsu4qz	440	28	from	from	IN
cord-268139-tgpsu4qz	440	29	three	three	CD
cord-268139-tgpsu4qz	440	30	separate	separate	JJ
cord-268139-tgpsu4qz	440	31	experiments	experiment	NNS
cord-268139-tgpsu4qz	440	32	.	.	.
cord-268139-tgpsu4qz	441	1	Data	data	NN
cord-268139-tgpsu4qz	441	2	analysis	analysis	NN
cord-268139-tgpsu4qz	441	3	was	be	VBD
cord-268139-tgpsu4qz	441	4	performed	perform	VBN
cord-268139-tgpsu4qz	441	5	using	use	VBG
cord-268139-tgpsu4qz	441	6	Smith	Smith	NNP
cord-268139-tgpsu4qz	441	7	's	's	POS
cord-268139-tgpsu4qz	441	8	Statistical	Statistical	NNP
cord-268139-tgpsu4qz	441	9	Package	Package	NNP
cord-268139-tgpsu4qz	441	10	-	-	HYPH
cord-268139-tgpsu4qz	441	11	Version	Version	NNP
cord-268139-tgpsu4qz	441	12	2.75	2.75	CD
cord-268139-tgpsu4qz	441	13	.	.	.
cord-268139-tgpsu4qz	442	1	A	a	DT
cord-268139-tgpsu4qz	442	2	two	two	CD
cord-268139-tgpsu4qz	442	3	-	-	HYPH
cord-268139-tgpsu4qz	442	4	sample	sample	NN
cord-268139-tgpsu4qz	442	5	t	t	NN
cord-268139-tgpsu4qz	442	6	test	test	NN
cord-268139-tgpsu4qz	442	7	was	be	VBD
cord-268139-tgpsu4qz	442	8	performed	perform	VBN
cord-268139-tgpsu4qz	442	9	for	for	IN
cord-268139-tgpsu4qz	442	10	each	each	DT
cord-268139-tgpsu4qz	442	11	virus	virus	NN
cord-268139-tgpsu4qz	442	12	(	(	-LRB-
cord-268139-tgpsu4qz	442	13	P	p	NN
cord-268139-tgpsu4qz	442	14	value	value	NN
cord-268139-tgpsu4qz	442	15	0.05	0.05	CD
cord-268139-tgpsu4qz	442	16	)	)	-RRB-
cord-268139-tgpsu4qz	442	17	.	.	.
cord-268139-tgpsu4qz	443	1	For	for	IN
cord-268139-tgpsu4qz	443	2	imaging	image	VBG
cord-268139-tgpsu4qz	443	3	relative	relative	JJ
cord-268139-tgpsu4qz	443	4	plaque	plaque	NN
cord-268139-tgpsu4qz	443	5	size	size	NN
cord-268139-tgpsu4qz	443	6	,	,	,
cord-268139-tgpsu4qz	443	7	confluent	confluent	JJ
cord-268139-tgpsu4qz	443	8	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	443	9	cells	cell	NNS
cord-268139-tgpsu4qz	443	10	in	in	IN
cord-268139-tgpsu4qz	443	11	60-mm	60-mm	CD
cord-268139-tgpsu4qz	443	12	culture	culture	NN
cord-268139-tgpsu4qz	443	13	dishes	dish	NNS
cord-268139-tgpsu4qz	443	14	were	be	VBD
cord-268139-tgpsu4qz	443	15	infected	infect	VBN
cord-268139-tgpsu4qz	443	16	with	with	IN
cord-268139-tgpsu4qz	443	17	serial	serial	JJ
cord-268139-tgpsu4qz	443	18	dilutions	dilution	NNS
cord-268139-tgpsu4qz	443	19	of	of	IN
cord-268139-tgpsu4qz	443	20	icwt	icwt	NNP
cord-268139-tgpsu4qz	443	21	,	,	,
cord-268139-tgpsu4qz	443	22	VUSB1	VUSB1	NNP
cord-268139-tgpsu4qz	443	23	,	,	,
cord-268139-tgpsu4qz	443	24	or	or	CC
cord-268139-tgpsu4qz	443	25	VUSB4	VUSB4	NNP
cord-268139-tgpsu4qz	443	26	virus	virus	NN
cord-268139-tgpsu4qz	443	27	and	and	CC
cord-268139-tgpsu4qz	443	28	then	then	RB
cord-268139-tgpsu4qz	443	29	were	be	VBD
cord-268139-tgpsu4qz	443	30	overlaid	overlay	VBN
cord-268139-tgpsu4qz	443	31	with	with	IN
cord-268139-tgpsu4qz	443	32	1	1	CD
cord-268139-tgpsu4qz	443	33	%	%	NN
cord-268139-tgpsu4qz	443	34	agar	agar	NN
cord-268139-tgpsu4qz	443	35	-DMEM	-dmem	NN
cord-268139-tgpsu4qz	443	36	supplemented	supplement	VBD
cord-268139-tgpsu4qz	443	37	with	with	IN
cord-268139-tgpsu4qz	443	38	10	10	CD
cord-268139-tgpsu4qz	443	39	%	%	NN
cord-268139-tgpsu4qz	443	40	FCS	FCS	NNP
cord-268139-tgpsu4qz	443	41	.	.	.
cord-268139-tgpsu4qz	444	1	At	at	IN
cord-268139-tgpsu4qz	444	2	15	15	CD
cord-268139-tgpsu4qz	444	3	h	h	NN
cord-268139-tgpsu4qz	444	4	p.i	p.i	NNP
cord-268139-tgpsu4qz	444	5	.	.	NNP
cord-268139-tgpsu4qz	444	6	,	,	,
cord-268139-tgpsu4qz	444	7	the	the	DT
cord-268139-tgpsu4qz	444	8	agar	agar	NN
cord-268139-tgpsu4qz	444	9	medium	medium	NN
cord-268139-tgpsu4qz	444	10	was	be	VBD
cord-268139-tgpsu4qz	444	11	removed	remove	VBN
cord-268139-tgpsu4qz	444	12	,	,	,
cord-268139-tgpsu4qz	444	13	and	and	CC
cord-268139-tgpsu4qz	444	14	the	the	DT
cord-268139-tgpsu4qz	444	15	cells	cell	NNS
cord-268139-tgpsu4qz	444	16	were	be	VBD
cord-268139-tgpsu4qz	444	17	fixed	fix	VBN
cord-268139-tgpsu4qz	444	18	for	for	IN
cord-268139-tgpsu4qz	444	19	30	30	CD
cord-268139-tgpsu4qz	444	20	min	min	NN
cord-268139-tgpsu4qz	444	21	using	use	VBG
cord-268139-tgpsu4qz	444	22	À20	À20	NNP
cord-268139-tgpsu4qz	444	23	-C	-C	:
cord-268139-tgpsu4qz	444	24	100	100	CD
cord-268139-tgpsu4qz	444	25	%	%	NN
cord-268139-tgpsu4qz	444	26	methanol	methanol	NN
cord-268139-tgpsu4qz	444	27	.	.	.
cord-268139-tgpsu4qz	445	1	Cells	cell	NNS
cord-268139-tgpsu4qz	445	2	were	be	VBD
cord-268139-tgpsu4qz	445	3	incubated	incubate	VBN
cord-268139-tgpsu4qz	445	4	in	in	IN
cord-268139-tgpsu4qz	445	5	PBS	PBS	NNP
cord-268139-tgpsu4qz	445	6	for	for	IN
cord-268139-tgpsu4qz	445	7	30	30	CD
cord-268139-tgpsu4qz	445	8	min	min	NN
cord-268139-tgpsu4qz	445	9	,	,	,
cord-268139-tgpsu4qz	445	10	and	and	CC
cord-268139-tgpsu4qz	445	11	then	then	RB
cord-268139-tgpsu4qz	445	12	images	image	NNS
cord-268139-tgpsu4qz	445	13	of	of	IN
cord-268139-tgpsu4qz	445	14	plaques	plaque	NNS
cord-268139-tgpsu4qz	445	15	were	be	VBD
cord-268139-tgpsu4qz	445	16	obtained	obtain	VBN
cord-268139-tgpsu4qz	445	17	on	on	IN
cord-268139-tgpsu4qz	445	18	a	a	DT
cord-268139-tgpsu4qz	445	19	Nikon	Nikon	NNP
cord-268139-tgpsu4qz	445	20	Eclipse	Eclipse	NNP
cord-268139-tgpsu4qz	445	21	TE2000-E	te2000-e	NN
cord-268139-tgpsu4qz	445	22	microscope	microscope	NN
cord-268139-tgpsu4qz	445	23	using	use	VBG
cord-268139-tgpsu4qz	445	24	a	a	DT
cord-268139-tgpsu4qz	445	25	10Â	10â	CD
cord-268139-tgpsu4qz	445	26	objective	objective	NN
cord-268139-tgpsu4qz	445	27	.	.	.
cord-268139-tgpsu4qz	446	1	Images	image	NNS
cord-268139-tgpsu4qz	446	2	were	be	VBD
cord-268139-tgpsu4qz	446	3	prepared	prepare	VBN
cord-268139-tgpsu4qz	446	4	using	use	VBG
cord-268139-tgpsu4qz	446	5	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	446	6	Photoshop	Photoshop	NNP
cord-268139-tgpsu4qz	446	7	8.0	8.0	CD
cord-268139-tgpsu4qz	446	8	(	(	-LRB-
cord-268139-tgpsu4qz	446	9	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	446	10	)	)	-RRB-
cord-268139-tgpsu4qz	446	11	.	.	.
cord-268139-tgpsu4qz	447	1	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	447	2	cells	cell	NNS
cord-268139-tgpsu4qz	447	3	(	(	-LRB-
cord-268139-tgpsu4qz	447	4	approximately	approximately	RB
cord-268139-tgpsu4qz	447	5	3	3	CD
cord-268139-tgpsu4qz	447	6	Â	â	CD
cord-268139-tgpsu4qz	447	7	10	10	CD
cord-268139-tgpsu4qz	447	8	6	6	CD
cord-268139-tgpsu4qz	447	9	cells	cell	NNS
cord-268139-tgpsu4qz	447	10	)	)	-RRB-
cord-268139-tgpsu4qz	447	11	were	be	VBD
cord-268139-tgpsu4qz	447	12	either	either	CC
cord-268139-tgpsu4qz	447	13	infected	infect	VBN
cord-268139-tgpsu4qz	447	14	at	at	IN
cord-268139-tgpsu4qz	447	15	an	an	DT
cord-268139-tgpsu4qz	447	16	MOI	MOI	NNP
cord-268139-tgpsu4qz	447	17	of	of	IN
cord-268139-tgpsu4qz	447	18	5	5	CD
cord-268139-tgpsu4qz	447	19	or	or	CC
cord-268139-tgpsu4qz	447	20	mock	mock	RB
cord-268139-tgpsu4qz	447	21	-	-	HYPH
cord-268139-tgpsu4qz	447	22	infected	infected	JJ
cord-268139-tgpsu4qz	447	23	using	use	VBG
cord-268139-tgpsu4qz	447	24	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	447	25	with	with	IN
cord-268139-tgpsu4qz	447	26	10	10	CD
cord-268139-tgpsu4qz	447	27	%	%	NN
cord-268139-tgpsu4qz	447	28	FCS	FCS	NNP
cord-268139-tgpsu4qz	447	29	.	.	.
cord-268139-tgpsu4qz	448	1	At	at	IN
cord-268139-tgpsu4qz	448	2	2.5	2.5	CD
cord-268139-tgpsu4qz	448	3	h	h	NN
cord-268139-tgpsu4qz	448	4	p.i	p.i	NNP
cord-268139-tgpsu4qz	448	5	.	.	NNP
cord-268139-tgpsu4qz	448	6	,	,	,
cord-268139-tgpsu4qz	448	7	the	the	DT
cord-268139-tgpsu4qz	448	8	medium	medium	NN
cord-268139-tgpsu4qz	448	9	was	be	VBD
cord-268139-tgpsu4qz	448	10	replaced	replace	VBN
cord-268139-tgpsu4qz	448	11	with	with	IN
cord-268139-tgpsu4qz	448	12	fresh	fresh	JJ
cord-268139-tgpsu4qz	448	13	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	448	14	lacking	lack	VBG
cord-268139-tgpsu4qz	448	15	methionine	methionine	NN
cord-268139-tgpsu4qz	448	16	and	and	CC
cord-268139-tgpsu4qz	448	17	cysteine	cysteine	NN
cord-268139-tgpsu4qz	448	18	and	and	CC
cord-268139-tgpsu4qz	448	19	supplemented	supplement	VBD
cord-268139-tgpsu4qz	448	20	with	with	IN
cord-268139-tgpsu4qz	448	21	5	5	CD
cord-268139-tgpsu4qz	448	22	%	%	NN
cord-268139-tgpsu4qz	448	23	FCS	FCS	NNP
cord-268139-tgpsu4qz	448	24	and	and	CC
cord-268139-tgpsu4qz	448	25	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	448	26	D	D	NNP
cord-268139-tgpsu4qz	448	27	(	(	-LRB-
cord-268139-tgpsu4qz	448	28	5	5	CD
cord-268139-tgpsu4qz	448	29	Ag	Ag	NNP
cord-268139-tgpsu4qz	448	30	/	/	SYM
cord-268139-tgpsu4qz	448	31	ml	ml	NN
cord-268139-tgpsu4qz	448	32	)	)	-RRB-
cord-268139-tgpsu4qz	448	33	.	.	.
cord-268139-tgpsu4qz	449	1	Proteins	protein	NNS
cord-268139-tgpsu4qz	449	2	were	be	VBD
cord-268139-tgpsu4qz	449	3	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	449	4	from	from	IN
cord-268139-tgpsu4qz	449	5	5	5	CD
cord-268139-tgpsu4qz	449	6	-8	-8	NNP
cord-268139-tgpsu4qz	449	7	h	h	NN
cord-268139-tgpsu4qz	449	8	p.i	p.i	NNP
cord-268139-tgpsu4qz	449	9	.	.	.
cord-268139-tgpsu4qz	449	10	using	use	VBG
cord-268139-tgpsu4qz	449	11	100	100	CD
cord-268139-tgpsu4qz	449	12	ACi	ACi	NNS
cord-268139-tgpsu4qz	449	13	/	/	SYM
cord-268139-tgpsu4qz	449	14	ml	ml	NNS
cord-268139-tgpsu4qz	449	15	of	of	IN
cord-268139-tgpsu4qz	449	16	[	[	-LRB-
cord-268139-tgpsu4qz	449	17	35	35	CD
cord-268139-tgpsu4qz	449	18	S]Met/	S]Met/	NNP
cord-268139-tgpsu4qz	449	19	Cys	Cys	NNP
cord-268139-tgpsu4qz	449	20	(	(	-LRB-
cord-268139-tgpsu4qz	449	21	Translabel	Translabel	NNP
cord-268139-tgpsu4qz	449	22	;	;	:
cord-268139-tgpsu4qz	449	23	ICN	ICN	NNP
cord-268139-tgpsu4qz	449	24	)	)	-RRB-
cord-268139-tgpsu4qz	449	25	.	.	.
cord-268139-tgpsu4qz	450	1	For	for	IN
cord-268139-tgpsu4qz	450	2	pulse	pulse	NN
cord-268139-tgpsu4qz	450	3	-	-	HYPH
cord-268139-tgpsu4qz	450	4	chase	chase	NN
cord-268139-tgpsu4qz	450	5	analysis	analysis	NN
cord-268139-tgpsu4qz	450	6	,	,	,
cord-268139-tgpsu4qz	450	7	proteins	protein	NNS
cord-268139-tgpsu4qz	450	8	were	be	VBD
cord-268139-tgpsu4qz	450	9	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	450	10	from	from	IN
cord-268139-tgpsu4qz	450	11	6	6	CD
cord-268139-tgpsu4qz	450	12	-	-	SYM
cord-268139-tgpsu4qz	450	13	6.5	6.5	CD
cord-268139-tgpsu4qz	450	14	h	h	NN
cord-268139-tgpsu4qz	450	15	p.i	p.i	NNP
cord-268139-tgpsu4qz	450	16	.	.	NNP
cord-268139-tgpsu4qz	450	17	and	and	CC
cord-268139-tgpsu4qz	450	18	then	then	RB
cord-268139-tgpsu4qz	450	19	chased	chase	VBD
cord-268139-tgpsu4qz	450	20	for	for	IN
cord-268139-tgpsu4qz	450	21	various	various	JJ
cord-268139-tgpsu4qz	450	22	times	time	NNS
cord-268139-tgpsu4qz	450	23	with	with	IN
cord-268139-tgpsu4qz	450	24	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	450	25	supplemented	supplement	VBD
cord-268139-tgpsu4qz	450	26	with	with	IN
cord-268139-tgpsu4qz	450	27	10	10	CD
cord-268139-tgpsu4qz	450	28	%	%	NN
cord-268139-tgpsu4qz	450	29	FCS	FCS	NNP
cord-268139-tgpsu4qz	450	30	and	and	CC
cord-268139-tgpsu4qz	450	31	cyclohexamide	cyclohexamide	NN
cord-268139-tgpsu4qz	451	1	(	(	-LRB-
cord-268139-tgpsu4qz	451	2	250	250	CD
cord-268139-tgpsu4qz	451	3	Ag	Ag	NNP
cord-268139-tgpsu4qz	451	4	/	/	SYM
cord-268139-tgpsu4qz	451	5	ml	ml	NN
cord-268139-tgpsu4qz	451	6	)	)	-RRB-
cord-268139-tgpsu4qz	451	7	.	.	.
cord-268139-tgpsu4qz	452	1	Cells	cell	NNS
cord-268139-tgpsu4qz	452	2	were	be	VBD
cord-268139-tgpsu4qz	452	3	washed	wash	VBN
cord-268139-tgpsu4qz	452	4	using	use	VBG
cord-268139-tgpsu4qz	452	5	500	500	CD
cord-268139-tgpsu4qz	452	6	Al	Al	NNP
cord-268139-tgpsu4qz	452	7	of	of	IN
cord-268139-tgpsu4qz	452	8	PBS	PBS	NNP
cord-268139-tgpsu4qz	452	9	and	and	CC
cord-268139-tgpsu4qz	452	10	then	then	RB
cord-268139-tgpsu4qz	452	11	lysed	lyse	VBD
cord-268139-tgpsu4qz	452	12	in	in	IN
cord-268139-tgpsu4qz	452	13	300	300	CD
cord-268139-tgpsu4qz	452	14	Al	Al	NNP
cord-268139-tgpsu4qz	452	15	of	of	IN
cord-268139-tgpsu4qz	452	16	lysis	lysis	NN
cord-268139-tgpsu4qz	452	17	buffer	buffer	NN
cord-268139-tgpsu4qz	452	18	containing	contain	VBG
cord-268139-tgpsu4qz	452	19	150	150	CD
cord-268139-tgpsu4qz	452	20	mM	mm	CD
cord-268139-tgpsu4qz	452	21	NaCl	nacl	NN
cord-268139-tgpsu4qz	452	22	,	,	,
cord-268139-tgpsu4qz	452	23	1	1	CD
cord-268139-tgpsu4qz	452	24	%	%	NN
cord-268139-tgpsu4qz	453	1	NP40	NP40	NNP
cord-268139-tgpsu4qz	453	2	,	,	,
cord-268139-tgpsu4qz	453	3	0.5	0.5	CD
cord-268139-tgpsu4qz	453	4	%	%	NN
cord-268139-tgpsu4qz	453	5	DOC	doc	NN
cord-268139-tgpsu4qz	453	6	,	,	,
cord-268139-tgpsu4qz	453	7	50	50	CD
cord-268139-tgpsu4qz	453	8	mM	mm	CD
cord-268139-tgpsu4qz	453	9	Tris	Tris	NNP
cord-268139-tgpsu4qz	453	10	pH	ph	NN
cord-268139-tgpsu4qz	453	11	8.0	8.0	CD
cord-268139-tgpsu4qz	453	12	.	.	.
cord-268139-tgpsu4qz	454	1	Lysates	Lysates	NNP
cord-268139-tgpsu4qz	454	2	were	be	VBD
cord-268139-tgpsu4qz	454	3	subjected	subject	VBN
cord-268139-tgpsu4qz	454	4	to	to	IN
cord-268139-tgpsu4qz	454	5	centrifugation	centrifugation	NN
cord-268139-tgpsu4qz	454	6	at	at	IN
cord-268139-tgpsu4qz	454	7	3500	3500	CD
cord-268139-tgpsu4qz	454	8	Â	â	NN
cord-268139-tgpsu4qz	454	9	g	g	NN
cord-268139-tgpsu4qz	454	10	to	to	TO
cord-268139-tgpsu4qz	454	11	remove	remove	VB
cord-268139-tgpsu4qz	454	12	cell	cell	NN
cord-268139-tgpsu4qz	454	13	nuclei	nucleus	NNS
cord-268139-tgpsu4qz	454	14	.	.	.
cord-268139-tgpsu4qz	455	1	Immunoprecipitations	Immunoprecipitations	NNPS
cord-268139-tgpsu4qz	455	2	were	be	VBD
cord-268139-tgpsu4qz	455	3	performed	perform	VBN
cord-268139-tgpsu4qz	455	4	in	in	IN
cord-268139-tgpsu4qz	455	5	a	a	DT
cord-268139-tgpsu4qz	455	6	final	final	JJ
cord-268139-tgpsu4qz	455	7	volume	volume	NN
cord-268139-tgpsu4qz	455	8	of	of	IN
cord-268139-tgpsu4qz	455	9	500	500	CD
cord-268139-tgpsu4qz	455	10	Al	Al	NNP
cord-268139-tgpsu4qz	455	11	using	use	VBG
cord-268139-tgpsu4qz	455	12	protein	protein	NN
cord-268139-tgpsu4qz	455	13	A	a	NN
cord-268139-tgpsu4qz	456	1	-Sepharose	-sepharose	WDT
cord-268139-tgpsu4qz	456	2	beads	bead	NNS
cord-268139-tgpsu4qz	456	3	(	(	-LRB-
cord-268139-tgpsu4qz	456	4	Sigma	Sigma	NNP
cord-268139-tgpsu4qz	456	5	)	)	-RRB-
cord-268139-tgpsu4qz	456	6	,	,	,
cord-268139-tgpsu4qz	456	7	100	100	CD
cord-268139-tgpsu4qz	456	8	Al	Al	NNP
cord-268139-tgpsu4qz	456	9	of	of	IN
cord-268139-tgpsu4qz	456	10	radiolabeled	radiolabeled	JJ
cord-268139-tgpsu4qz	456	11	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	456	12	lysate	lysate	NN
cord-268139-tgpsu4qz	456	13	(	(	-LRB-
cord-268139-tgpsu4qz	456	14	derived	derive	VBN
cord-268139-tgpsu4qz	456	15	from	from	IN
cord-268139-tgpsu4qz	456	16	approximately	approximately	RB
cord-268139-tgpsu4qz	456	17	10	10	CD
cord-268139-tgpsu4qz	456	18	6	6	CD
cord-268139-tgpsu4qz	456	19	cells	cell	NNS
cord-268139-tgpsu4qz	456	20	)	)	-RRB-
cord-268139-tgpsu4qz	456	21	which	which	WDT
cord-268139-tgpsu4qz	456	22	was	be	VBD
cord-268139-tgpsu4qz	456	23	boiled	boil	VBN
cord-268139-tgpsu4qz	456	24	for	for	IN
cord-268139-tgpsu4qz	456	25	5	5	CD
cord-268139-tgpsu4qz	456	26	min	min	NN
cord-268139-tgpsu4qz	456	27	in	in	IN
cord-268139-tgpsu4qz	456	28	1	1	CD
cord-268139-tgpsu4qz	456	29	%	%	NN
cord-268139-tgpsu4qz	456	30	SDS	SDS	NNP
cord-268139-tgpsu4qz	456	31	,	,	,
cord-268139-tgpsu4qz	456	32	and	and	CC
cord-268139-tgpsu4qz	456	33	2	2	CD
cord-268139-tgpsu4qz	456	34	-10	-10	IN
cord-268139-tgpsu4qz	456	35	Al	Al	NNP
cord-268139-tgpsu4qz	456	36	of	of	IN
cord-268139-tgpsu4qz	456	37	polyclonal	polyclonal	JJ
cord-268139-tgpsu4qz	456	38	antisera	antisera	NN
cord-268139-tgpsu4qz	456	39	in	in	IN
cord-268139-tgpsu4qz	456	40	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	456	41	buffer	buffer	NN
cord-268139-tgpsu4qz	456	42	containing	contain	VBG
cord-268139-tgpsu4qz	456	43	300	300	CD
cord-268139-tgpsu4qz	456	44	mM	mm	CD
cord-268139-tgpsu4qz	456	45	NaCl	nacl	NN
cord-268139-tgpsu4qz	456	46	,	,	,
cord-268139-tgpsu4qz	456	47	0.1	0.1	CD
cord-268139-tgpsu4qz	456	48	%	%	NN
cord-268139-tgpsu4qz	456	49	SDS	SDS	NNP
cord-268139-tgpsu4qz	456	50	,	,	,
cord-268139-tgpsu4qz	456	51	0.5	0.5	CD
cord-268139-tgpsu4qz	456	52	%	%	NN
cord-268139-tgpsu4qz	456	53	TX-100	TX-100	NNP
cord-268139-tgpsu4qz	456	54	,	,	,
cord-268139-tgpsu4qz	456	55	4	4	CD
cord-268139-tgpsu4qz	456	56	mM	mm	CD
cord-268139-tgpsu4qz	456	57	EDTA	EDTA	NNP
cord-268139-tgpsu4qz	456	58	,	,	,
cord-268139-tgpsu4qz	456	59	0.1	0.1	CD
cord-268139-tgpsu4qz	456	60	%	%	NN
cord-268139-tgpsu4qz	456	61	DTT	DTT	NNP
cord-268139-tgpsu4qz	456	62	,	,	,
cord-268139-tgpsu4qz	456	63	and	and	CC
cord-268139-tgpsu4qz	456	64	50	50	CD
cord-268139-tgpsu4qz	456	65	mM	mm	CD
cord-268139-tgpsu4qz	456	66	Tris	Tris	NNP
cord-268139-tgpsu4qz	456	67	pH	pH	NNP
cord-268139-tgpsu4qz	456	68	7.4	7.4	CD
cord-268139-tgpsu4qz	456	69	.	.	.
cord-268139-tgpsu4qz	457	1	Immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	457	2	reactions	reaction	NNS
cord-268139-tgpsu4qz	457	3	were	be	VBD
cord-268139-tgpsu4qz	457	4	incubated	incubate	VBN
cord-268139-tgpsu4qz	457	5	at	at	IN
cord-268139-tgpsu4qz	457	6	4	4	CD
cord-268139-tgpsu4qz	457	7	-C	-C	NNS
cord-268139-tgpsu4qz	457	8	for	for	IN
cord-268139-tgpsu4qz	457	9	4	4	CD
cord-268139-tgpsu4qz	457	10	h	h	NN
cord-268139-tgpsu4qz	457	11	with	with	IN
cord-268139-tgpsu4qz	457	12	rotation	rotation	NN
cord-268139-tgpsu4qz	457	13	.	.	.
cord-268139-tgpsu4qz	458	1	Protein	protein	NN
cord-268139-tgpsu4qz	458	2	-	-	HYPH
cord-268139-tgpsu4qz	458	3	bead	bead	NN
cord-268139-tgpsu4qz	458	4	conjugates	conjugate	NNS
cord-268139-tgpsu4qz	458	5	were	be	VBD
cord-268139-tgpsu4qz	458	6	washed	wash	VBN
cord-268139-tgpsu4qz	458	7	three	three	CD
cord-268139-tgpsu4qz	458	8	times	time	NNS
cord-268139-tgpsu4qz	458	9	in	in	IN
cord-268139-tgpsu4qz	458	10	immunoprecipitation	immunoprecipitation	NN
cord-268139-tgpsu4qz	458	11	buffer	buffer	NN
cord-268139-tgpsu4qz	458	12	,	,	,
cord-268139-tgpsu4qz	458	13	and	and	CC
cord-268139-tgpsu4qz	458	14	proteins	protein	NNS
cord-268139-tgpsu4qz	458	15	were	be	VBD
cord-268139-tgpsu4qz	458	16	eluted	elute	VBN
cord-268139-tgpsu4qz	458	17	from	from	IN
cord-268139-tgpsu4qz	458	18	beads	bead	NNS
cord-268139-tgpsu4qz	458	19	by	by	IN
cord-268139-tgpsu4qz	458	20	boiling	boil	VBG
cord-268139-tgpsu4qz	458	21	for	for	IN
cord-268139-tgpsu4qz	458	22	5	5	CD
cord-268139-tgpsu4qz	458	23	min	min	NN
cord-268139-tgpsu4qz	458	24	in	in	IN
cord-268139-tgpsu4qz	458	25	2Â	2â	CD
cord-268139-tgpsu4qz	458	26	protein	protein	NN
cord-268139-tgpsu4qz	458	27	loading	loading	NN
cord-268139-tgpsu4qz	458	28	buffer	buffer	NN
cord-268139-tgpsu4qz	458	29	(	(	-LRB-
cord-268139-tgpsu4qz	458	30	200	200	CD
cord-268139-tgpsu4qz	458	31	mM	mm	CD
cord-268139-tgpsu4qz	458	32	DTT	DTT	NNP
cord-268139-tgpsu4qz	458	33	,	,	,
cord-268139-tgpsu4qz	458	34	100	100	CD
cord-268139-tgpsu4qz	458	35	mM	mm	CD
cord-268139-tgpsu4qz	458	36	Tris	Tris	NNP
cord-268139-tgpsu4qz	458	37	pH	pH	NNP
cord-268139-tgpsu4qz	458	38	6.8	6.8	CD
cord-268139-tgpsu4qz	458	39	,	,	,
cord-268139-tgpsu4qz	458	40	0.04	0.04	CD
cord-268139-tgpsu4qz	458	41	%	%	NN
cord-268139-tgpsu4qz	458	42	bromophenol	bromophenol	NN
cord-268139-tgpsu4qz	458	43	blue	blue	JJ
cord-268139-tgpsu4qz	458	44	,	,	,
cord-268139-tgpsu4qz	458	45	20	20	CD
cord-268139-tgpsu4qz	458	46	%	%	NN
cord-268139-tgpsu4qz	458	47	glycerol	glycerol	NN
cord-268139-tgpsu4qz	458	48	)	)	-RRB-
cord-268139-tgpsu4qz	458	49	.	.	.
cord-268139-tgpsu4qz	459	1	Proteins	protein	NNS
cord-268139-tgpsu4qz	459	2	were	be	VBD
cord-268139-tgpsu4qz	459	3	resolved	resolve	VBN
cord-268139-tgpsu4qz	459	4	by	by	IN
cord-268139-tgpsu4qz	459	5	SDS	SDS	NNP
cord-268139-tgpsu4qz	459	6	-	-	HYPH
cord-268139-tgpsu4qz	459	7	PAGE	PAGE	NNP
cord-268139-tgpsu4qz	459	8	in	in	IN
cord-268139-tgpsu4qz	459	9	5	5	CD
cord-268139-tgpsu4qz	459	10	-18	-18	CD
cord-268139-tgpsu4qz	459	11	%	%	NN
cord-268139-tgpsu4qz	459	12	polyacrylamide	polyacrylamide	NN
cord-268139-tgpsu4qz	459	13	gradient	gradient	NN
cord-268139-tgpsu4qz	459	14	gels	gel	NNS
cord-268139-tgpsu4qz	459	15	and	and	CC
cord-268139-tgpsu4qz	459	16	analyzed	analyze	VBN
cord-268139-tgpsu4qz	459	17	by	by	IN
cord-268139-tgpsu4qz	459	18	fluorography	fluorography	NNP
cord-268139-tgpsu4qz	459	19	.	.	.
cord-268139-tgpsu4qz	460	1	The	the	DT
cord-268139-tgpsu4qz	460	2	[	[	-LRB-
cord-268139-tgpsu4qz	460	3	14	14	CD
cord-268139-tgpsu4qz	460	4	C	C	NNP
cord-268139-tgpsu4qz	460	5	]	]	-RRB-
cord-268139-tgpsu4qz	460	6	high	high	JJ
cord-268139-tgpsu4qz	460	7	molecular	molecular	JJ
cord-268139-tgpsu4qz	460	8	weight	weight	NN
cord-268139-tgpsu4qz	460	9	standard	standard	NN
cord-268139-tgpsu4qz	460	10	(	(	-LRB-
cord-268139-tgpsu4qz	460	11	Gibco	Gibco	NNP
cord-268139-tgpsu4qz	460	12	)	)	-RRB-
cord-268139-tgpsu4qz	460	13	and	and	CC
cord-268139-tgpsu4qz	460	14	full	full	JJ
cord-268139-tgpsu4qz	460	15	-	-	HYPH
cord-268139-tgpsu4qz	460	16	range	range	NN
cord-268139-tgpsu4qz	460	17	rainbow	rainbow	NN
cord-268139-tgpsu4qz	460	18	marker	marker	NN
cord-268139-tgpsu4qz	460	19	(	(	-LRB-
cord-268139-tgpsu4qz	460	20	Invitrogen	Invitrogen	NNP
cord-268139-tgpsu4qz	460	21	)	)	-RRB-
cord-268139-tgpsu4qz	460	22	were	be	VBD
cord-268139-tgpsu4qz	460	23	used	use	VBN
cord-268139-tgpsu4qz	460	24	as	as	IN
cord-268139-tgpsu4qz	460	25	molecular	molecular	JJ
cord-268139-tgpsu4qz	460	26	weight	weight	NN
cord-268139-tgpsu4qz	460	27	standards	standard	NNS
cord-268139-tgpsu4qz	460	28	.	.	.
cord-268139-tgpsu4qz	461	1	Images	image	NNS
cord-268139-tgpsu4qz	461	2	were	be	VBD
cord-268139-tgpsu4qz	461	3	prepared	prepare	VBN
cord-268139-tgpsu4qz	461	4	using	use	VBG
cord-268139-tgpsu4qz	461	5	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	461	6	Photoshop	Photoshop	NNP
cord-268139-tgpsu4qz	461	7	8.0	8.0	CD
cord-268139-tgpsu4qz	461	8	(	(	-LRB-
cord-268139-tgpsu4qz	461	9	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	461	10	)	)	-RRB-
cord-268139-tgpsu4qz	461	11	.	.	.
cord-268139-tgpsu4qz	462	1	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	462	2	cells	cell	NNS
cord-268139-tgpsu4qz	462	3	cultured	culture	VBN
cord-268139-tgpsu4qz	462	4	on	on	IN
cord-268139-tgpsu4qz	462	5	glass	glass	NN
cord-268139-tgpsu4qz	462	6	coverslips	coverslip	NNS
cord-268139-tgpsu4qz	462	7	were	be	VBD
cord-268139-tgpsu4qz	462	8	either	either	DT
cord-268139-tgpsu4qz	462	9	mock	mock	NN
cord-268139-tgpsu4qz	462	10	-	-	HYPH
cord-268139-tgpsu4qz	462	11	infected	infect	VBN
cord-268139-tgpsu4qz	462	12	or	or	CC
cord-268139-tgpsu4qz	462	13	infected	infect	VBN
cord-268139-tgpsu4qz	462	14	at	at	IN
cord-268139-tgpsu4qz	462	15	an	an	DT
cord-268139-tgpsu4qz	462	16	MOI	MOI	NNP
cord-268139-tgpsu4qz	462	17	of	of	IN
cord-268139-tgpsu4qz	462	18	5	5	CD
cord-268139-tgpsu4qz	462	19	at	at	IN
cord-268139-tgpsu4qz	462	20	25	25	CD
cord-268139-tgpsu4qz	462	21	-C	-C	NNS
cord-268139-tgpsu4qz	462	22	for	for	IN
cord-268139-tgpsu4qz	462	23	30	30	CD
cord-268139-tgpsu4qz	462	24	min	min	NN
cord-268139-tgpsu4qz	462	25	.	.	.
cord-268139-tgpsu4qz	463	1	Following	follow	VBG
cord-268139-tgpsu4qz	463	2	virus	virus	NN
cord-268139-tgpsu4qz	463	3	adsorption	adsorption	NN
cord-268139-tgpsu4qz	463	4	,	,	,
cord-268139-tgpsu4qz	463	5	infected	infected	JJ
cord-268139-tgpsu4qz	463	6	medium	medium	NN
cord-268139-tgpsu4qz	463	7	was	be	VBD
cord-268139-tgpsu4qz	463	8	replaced	replace	VBN
cord-268139-tgpsu4qz	463	9	with	with	IN
cord-268139-tgpsu4qz	463	10	pre	pre	JJ
cord-268139-tgpsu4qz	463	11	-	-	JJ
cord-268139-tgpsu4qz	463	12	warmed	warm	VBN
cord-268139-tgpsu4qz	463	13	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	463	14	with	with	IN
cord-268139-tgpsu4qz	463	15	10	10	CD
cord-268139-tgpsu4qz	463	16	%	%	NN
cord-268139-tgpsu4qz	463	17	FCS	FCS	NNP
cord-268139-tgpsu4qz	463	18	,	,	,
cord-268139-tgpsu4qz	463	19	and	and	CC
cord-268139-tgpsu4qz	463	20	cells	cell	NNS
cord-268139-tgpsu4qz	463	21	were	be	VBD
cord-268139-tgpsu4qz	463	22	incubated	incubate	VBN
cord-268139-tgpsu4qz	463	23	at	at	IN
cord-268139-tgpsu4qz	463	24	37	37	CD
cord-268139-tgpsu4qz	463	25	-C.	-c.	NN
cord-268139-tgpsu4qz	464	1	At	at	IN
cord-268139-tgpsu4qz	464	2	7	7	CD
cord-268139-tgpsu4qz	464	3	h	h	NN
cord-268139-tgpsu4qz	464	4	p.i	p.i	NNP
cord-268139-tgpsu4qz	464	5	.	.	NNP
cord-268139-tgpsu4qz	464	6	,	,	,
cord-268139-tgpsu4qz	464	7	cells	cell	NNS
cord-268139-tgpsu4qz	464	8	were	be	VBD
cord-268139-tgpsu4qz	464	9	fixed	fix	VBN
cord-268139-tgpsu4qz	464	10	and	and	CC
cord-268139-tgpsu4qz	464	11	permeablized	permeablize	VBN
cord-268139-tgpsu4qz	464	12	with	with	IN
cord-268139-tgpsu4qz	464	13	À20	À20	NNP
cord-268139-tgpsu4qz	464	14	-C	-C	VBZ
cord-268139-tgpsu4qz	464	15	100	100	CD
cord-268139-tgpsu4qz	464	16	%	%	NN
cord-268139-tgpsu4qz	464	17	methanol	methanol	NN
cord-268139-tgpsu4qz	464	18	for	for	IN
cord-268139-tgpsu4qz	464	19	a	a	DT
cord-268139-tgpsu4qz	464	20	minimum	minimum	NN
cord-268139-tgpsu4qz	464	21	of	of	IN
cord-268139-tgpsu4qz	464	22	30	30	CD
cord-268139-tgpsu4qz	464	23	min	min	NN
cord-268139-tgpsu4qz	464	24	.	.	.
cord-268139-tgpsu4qz	465	1	Indirect	indirect	JJ
cord-268139-tgpsu4qz	465	2	immunofluorescence	immunofluorescence	NN
cord-268139-tgpsu4qz	465	3	assays	assay	NNS
cord-268139-tgpsu4qz	465	4	were	be	VBD
cord-268139-tgpsu4qz	465	5	performed	perform	VBN
cord-268139-tgpsu4qz	465	6	as	as	IN
cord-268139-tgpsu4qz	465	7	previously	previously	RB
cord-268139-tgpsu4qz	465	8	described	describe	VBN
cord-268139-tgpsu4qz	465	9	(	(	-LRB-
cord-268139-tgpsu4qz	465	10	Bost	Bost	NNP
cord-268139-tgpsu4qz	465	11	et	et	NNP
cord-268139-tgpsu4qz	465	12	al	al	NNP
cord-268139-tgpsu4qz	465	13	.	.	NNP
cord-268139-tgpsu4qz	465	14	,	,	,
cord-268139-tgpsu4qz	465	15	2000	2000	CD
cord-268139-tgpsu4qz	465	16	)	)	-RRB-
cord-268139-tgpsu4qz	465	17	.	.	.
cord-268139-tgpsu4qz	466	1	GP3	GP3	NNP
cord-268139-tgpsu4qz	466	2	was	be	VBD
cord-268139-tgpsu4qz	466	3	used	use	VBN
cord-268139-tgpsu4qz	466	4	at	at	IN
cord-268139-tgpsu4qz	466	5	1:2000	1:2000	CD
cord-268139-tgpsu4qz	466	6	dilution	dilution	NN
cord-268139-tgpsu4qz	466	7	,	,	,
cord-268139-tgpsu4qz	466	8	and	and	CC
cord-268139-tgpsu4qz	466	9	all	all	DT
cord-268139-tgpsu4qz	466	10	rabbit	rabbit	NN
cord-268139-tgpsu4qz	466	11	polyclonal	polyclonal	JJ
cord-268139-tgpsu4qz	466	12	antisera	antisera	NNP
cord-268139-tgpsu4qz	466	13	were	be	VBD
cord-268139-tgpsu4qz	466	14	used	use	VBN
cord-268139-tgpsu4qz	466	15	at	at	IN
cord-268139-tgpsu4qz	466	16	1:100	1:100	CD
cord-268139-tgpsu4qz	466	17	dilution	dilution	NN
cord-268139-tgpsu4qz	466	18	.	.	.
cord-268139-tgpsu4qz	467	1	Murine	murine	JJ
cord-268139-tgpsu4qz	467	2	monoclonal	monoclonal	JJ
cord-268139-tgpsu4qz	467	3	antibodies	antibody	NNS
cord-268139-tgpsu4qz	467	4	were	be	VBD
cord-268139-tgpsu4qz	467	5	used	use	VBN
cord-268139-tgpsu4qz	467	6	at	at	IN
cord-268139-tgpsu4qz	467	7	1:1000	1:1000	CD
cord-268139-tgpsu4qz	467	8	dilution	dilution	NN
cord-268139-tgpsu4qz	467	9	.	.	.
cord-268139-tgpsu4qz	468	1	Secondary	secondary	JJ
cord-268139-tgpsu4qz	468	2	antibodies	antibody	NNS
cord-268139-tgpsu4qz	468	3	conjugated	conjugate	VBN
cord-268139-tgpsu4qz	468	4	to	to	IN
cord-268139-tgpsu4qz	468	5	fluorophores	fluorophore	NNS
cord-268139-tgpsu4qz	468	6	(	(	-LRB-
cord-268139-tgpsu4qz	468	7	Molecular	Molecular	NNP
cord-268139-tgpsu4qz	468	8	Probes	Probes	NNPS
cord-268139-tgpsu4qz	468	9	)	)	-RRB-
cord-268139-tgpsu4qz	468	10	were	be	VBD
cord-268139-tgpsu4qz	468	11	used	use	VBN
cord-268139-tgpsu4qz	468	12	at	at	IN
cord-268139-tgpsu4qz	468	13	1:1000	1:1000	CD
cord-268139-tgpsu4qz	468	14	and	and	CC
cord-268139-tgpsu4qz	468	15	included	include	VBD
cord-268139-tgpsu4qz	468	16	a	a	DT
cord-268139-tgpsu4qz	468	17	-	-	HYPH
cord-268139-tgpsu4qz	468	18	guinea	guinea	NN
cord-268139-tgpsu4qz	468	19	pig	pig	NN
cord-268139-tgpsu4qz	468	20	-	-	:
cord-268139-tgpsu4qz	468	21	Alexa	Alexa	NNP
cord-268139-tgpsu4qz	468	22	546	546	CD
cord-268139-tgpsu4qz	468	23	,	,	,
cord-268139-tgpsu4qz	468	24	arabbit	arabbit	NNP
cord-268139-tgpsu4qz	468	25	-	-	:
cord-268139-tgpsu4qz	468	26	Alexa	Alexa	NNP
cord-268139-tgpsu4qz	468	27	488	488	CD
cord-268139-tgpsu4qz	468	28	,	,	,
cord-268139-tgpsu4qz	468	29	and	and	CC
cord-268139-tgpsu4qz	468	30	a	a	DT
cord-268139-tgpsu4qz	468	31	-	-	HYPH
cord-268139-tgpsu4qz	468	32	murine	murine	NN
cord-268139-tgpsu4qz	468	33	-	-	HYPH
cord-268139-tgpsu4qz	468	34	Alexa	Alexa	NNP
cord-268139-tgpsu4qz	468	35	633	633	CD
cord-268139-tgpsu4qz	468	36	.	.	.
cord-268139-tgpsu4qz	469	1	Immunofluorescence	Immunofluorescence	NNP
cord-268139-tgpsu4qz	469	2	was	be	VBD
cord-268139-tgpsu4qz	469	3	detected	detect	VBN
cord-268139-tgpsu4qz	469	4	using	use	VBG
cord-268139-tgpsu4qz	469	5	a	a	DT
cord-268139-tgpsu4qz	469	6	Zeiss	Zeiss	NNP
cord-268139-tgpsu4qz	469	7	LSM	LSM	NNP
cord-268139-tgpsu4qz	469	8	510	510	CD
cord-268139-tgpsu4qz	469	9	laser	laser	NN
cord-268139-tgpsu4qz	469	10	scanning	scanning	NN
cord-268139-tgpsu4qz	469	11	confocal	confocal	JJ
cord-268139-tgpsu4qz	469	12	microscope	microscope	NN
cord-268139-tgpsu4qz	469	13	with	with	IN
cord-268139-tgpsu4qz	469	14	a	a	DT
cord-268139-tgpsu4qz	469	15	40Â	40â	CD
cord-268139-tgpsu4qz	469	16	oil	oil	NN
cord-268139-tgpsu4qz	469	17	immersion	immersion	NN
cord-268139-tgpsu4qz	469	18	objective	objective	NN
cord-268139-tgpsu4qz	469	19	.	.	.
cord-268139-tgpsu4qz	470	1	Image	image	NN
cord-268139-tgpsu4qz	470	2	analysis	analysis	NN
cord-268139-tgpsu4qz	470	3	and	and	CC
cord-268139-tgpsu4qz	470	4	merging	merging	NN
cord-268139-tgpsu4qz	470	5	were	be	VBD
cord-268139-tgpsu4qz	470	6	performed	perform	VBN
cord-268139-tgpsu4qz	470	7	using	use	VBG
cord-268139-tgpsu4qz	470	8	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	470	9	Photoshop	Photoshop	NNP
cord-268139-tgpsu4qz	470	10	8.0	8.0	CD
cord-268139-tgpsu4qz	470	11	(	(	-LRB-
cord-268139-tgpsu4qz	470	12	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	470	13	)	)	-RRB-
cord-268139-tgpsu4qz	470	14	.	.	.
cord-268139-tgpsu4qz	471	1	DBT-9	DBT-9	NNP
cord-268139-tgpsu4qz	471	2	cells	cell	NNS
cord-268139-tgpsu4qz	471	3	(	(	-LRB-
cord-268139-tgpsu4qz	471	4	approximately	approximately	RB
cord-268139-tgpsu4qz	471	5	10	10	CD
cord-268139-tgpsu4qz	471	6	6	6	CD
cord-268139-tgpsu4qz	471	7	cells	cell	NNS
cord-268139-tgpsu4qz	471	8	)	)	-RRB-
cord-268139-tgpsu4qz	471	9	were	be	VBD
cord-268139-tgpsu4qz	471	10	infected	infect	VBN
cord-268139-tgpsu4qz	471	11	at	at	IN
cord-268139-tgpsu4qz	471	12	an	an	DT
cord-268139-tgpsu4qz	471	13	MOI	MOI	NNP
cord-268139-tgpsu4qz	471	14	of	of	IN
cord-268139-tgpsu4qz	471	15	5	5	CD
cord-268139-tgpsu4qz	471	16	with	with	IN
cord-268139-tgpsu4qz	471	17	icwt	icwt	NNP
cord-268139-tgpsu4qz	471	18	or	or	CC
cord-268139-tgpsu4qz	471	19	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	471	20	mutant	mutant	JJ
cord-268139-tgpsu4qz	471	21	viruses	virus	NNS
cord-268139-tgpsu4qz	471	22	.	.	.
cord-268139-tgpsu4qz	472	1	Virus	virus	NN
cord-268139-tgpsu4qz	472	2	inoculum	inoculum	NN
cord-268139-tgpsu4qz	472	3	was	be	VBD
cord-268139-tgpsu4qz	472	4	removed	remove	VBN
cord-268139-tgpsu4qz	472	5	following	follow	VBG
cord-268139-tgpsu4qz	472	6	30	30	CD
cord-268139-tgpsu4qz	472	7	min	min	NN
cord-268139-tgpsu4qz	472	8	adsorption	adsorption	NN
cord-268139-tgpsu4qz	472	9	at	at	IN
cord-268139-tgpsu4qz	472	10	25	25	CD
cord-268139-tgpsu4qz	472	11	-C	-C	NNS
cord-268139-tgpsu4qz	472	12	,	,	,
cord-268139-tgpsu4qz	472	13	and	and	CC
cord-268139-tgpsu4qz	472	14	cells	cell	NNS
cord-268139-tgpsu4qz	472	15	were	be	VBD
cord-268139-tgpsu4qz	472	16	washed	wash	VBN
cord-268139-tgpsu4qz	472	17	three	three	CD
cord-268139-tgpsu4qz	472	18	times	time	NNS
cord-268139-tgpsu4qz	472	19	with	with	IN
cord-268139-tgpsu4qz	472	20	PBS	PBS	NNP
cord-268139-tgpsu4qz	472	21	.	.	.
cord-268139-tgpsu4qz	473	1	Cells	cell	NNS
cord-268139-tgpsu4qz	473	2	were	be	VBD
cord-268139-tgpsu4qz	473	3	then	then	RB
cord-268139-tgpsu4qz	473	4	incubated	incubate	VBN
cord-268139-tgpsu4qz	473	5	for	for	IN
cord-268139-tgpsu4qz	473	6	various	various	JJ
cord-268139-tgpsu4qz	473	7	times	time	NNS
cord-268139-tgpsu4qz	473	8	in	in	IN
cord-268139-tgpsu4qz	473	9	fresh	fresh	JJ
cord-268139-tgpsu4qz	473	10	pre	pre	JJ
cord-268139-tgpsu4qz	473	11	-	-	JJ
cord-268139-tgpsu4qz	473	12	warmed	warm	VBN
cord-268139-tgpsu4qz	473	13	DMEM	DMEM	NNP
cord-268139-tgpsu4qz	473	14	with	with	IN
cord-268139-tgpsu4qz	473	15	10	10	CD
cord-268139-tgpsu4qz	473	16	%	%	NN
cord-268139-tgpsu4qz	473	17	FCS	FCS	NNP
cord-268139-tgpsu4qz	473	18	at	at	IN
cord-268139-tgpsu4qz	473	19	37	37	CD
cord-268139-tgpsu4qz	473	20	-C.	-c.	NN
cord-268139-tgpsu4qz	474	1	At	at	IN
cord-268139-tgpsu4qz	474	2	30	30	CD
cord-268139-tgpsu4qz	474	3	min	min	NN
cord-268139-tgpsu4qz	474	4	prior	prior	RB
cord-268139-tgpsu4qz	474	5	to	to	IN
cord-268139-tgpsu4qz	474	6	addition	addition	NN
cord-268139-tgpsu4qz	474	7	of	of	IN
cord-268139-tgpsu4qz	474	8	radiolabel	radiolabel	NN
cord-268139-tgpsu4qz	474	9	,	,	,
cord-268139-tgpsu4qz	474	10	cell	cell	NN
cord-268139-tgpsu4qz	474	11	medium	medium	NN
cord-268139-tgpsu4qz	474	12	was	be	VBD
cord-268139-tgpsu4qz	474	13	supplemented	supplement	VBN
cord-268139-tgpsu4qz	474	14	with	with	IN
cord-268139-tgpsu4qz	474	15	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	474	16	D	D	NNP
cord-268139-tgpsu4qz	474	17	(	(	-LRB-
cord-268139-tgpsu4qz	474	18	5	5	CD
cord-268139-tgpsu4qz	474	19	Ag	Ag	NNP
cord-268139-tgpsu4qz	474	20	/	/	SYM
cord-268139-tgpsu4qz	474	21	ml	ml	NN
cord-268139-tgpsu4qz	474	22	)	)	-RRB-
cord-268139-tgpsu4qz	474	23	.	.	.
cord-268139-tgpsu4qz	475	1	Viral	Viral	NNP
cord-268139-tgpsu4qz	475	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	475	3	was	be	VBD
cord-268139-tgpsu4qz	475	4	metabolically	metabolically	RB
cord-268139-tgpsu4qz	475	5	labeled	label	VBN
cord-268139-tgpsu4qz	475	6	in	in	IN
cord-268139-tgpsu4qz	475	7	the	the	DT
cord-268139-tgpsu4qz	475	8	presence	presence	NN
cord-268139-tgpsu4qz	475	9	of	of	IN
cord-268139-tgpsu4qz	475	10	Actinomycin	Actinomycin	NNP
cord-268139-tgpsu4qz	475	11	D	D	NNP
cord-268139-tgpsu4qz	475	12	using	use	VBG
cord-268139-tgpsu4qz	475	13	100	100	CD
cord-268139-tgpsu4qz	475	14	ACi	ACi	NNS
cord-268139-tgpsu4qz	475	15	/	/	SYM
cord-268139-tgpsu4qz	475	16	ml	ml	NN
cord-268139-tgpsu4qz	475	17	[	[	-LRB-
cord-268139-tgpsu4qz	475	18	3	3	CD
cord-268139-tgpsu4qz	475	19	H]uridine	H]uridine	NNP
cord-268139-tgpsu4qz	475	20	.	.	.
cord-268139-tgpsu4qz	476	1	To	to	TO
cord-268139-tgpsu4qz	476	2	harvest	harvest	VB
cord-268139-tgpsu4qz	476	3	viral	viral	JJ
cord-268139-tgpsu4qz	476	4	RNA	RNA	NNP
cord-268139-tgpsu4qz	476	5	,	,	,
cord-268139-tgpsu4qz	476	6	cells	cell	NNS
cord-268139-tgpsu4qz	476	7	were	be	VBD
cord-268139-tgpsu4qz	476	8	washed	wash	VBN
cord-268139-tgpsu4qz	476	9	twice	twice	RB
cord-268139-tgpsu4qz	476	10	with	with	IN
cord-268139-tgpsu4qz	476	11	PBS	PBS	NNP
cord-268139-tgpsu4qz	476	12	and	and	CC
cord-268139-tgpsu4qz	476	13	then	then	RB
cord-268139-tgpsu4qz	476	14	lysed	lyse	VBN
cord-268139-tgpsu4qz	476	15	with	with	IN
cord-268139-tgpsu4qz	476	16	300	300	CD
cord-268139-tgpsu4qz	476	17	Al	Al	NNP
cord-268139-tgpsu4qz	476	18	of	of	IN
cord-268139-tgpsu4qz	476	19	cell	cell	NN
cord-268139-tgpsu4qz	476	20	lysis	lysis	NN
cord-268139-tgpsu4qz	476	21	buffer	buffer	NN
cord-268139-tgpsu4qz	476	22	(	(	-LRB-
cord-268139-tgpsu4qz	476	23	150	150	CD
cord-268139-tgpsu4qz	476	24	mM	mm	CD
cord-268139-tgpsu4qz	476	25	NaCl	nacl	NN
cord-268139-tgpsu4qz	476	26	,	,	,
cord-268139-tgpsu4qz	476	27	1	1	CD
cord-268139-tgpsu4qz	476	28	%	%	NN
cord-268139-tgpsu4qz	477	1	NP40	NP40	NNP
cord-268139-tgpsu4qz	477	2	,	,	,
cord-268139-tgpsu4qz	477	3	0.5	0.5	CD
cord-268139-tgpsu4qz	477	4	%	%	NN
cord-268139-tgpsu4qz	477	5	DOC	doc	NN
cord-268139-tgpsu4qz	477	6	and	and	CC
cord-268139-tgpsu4qz	478	1	50	50	CD
cord-268139-tgpsu4qz	478	2	mM	mm	CD
cord-268139-tgpsu4qz	478	3	Tris	Tris	NNP
cord-268139-tgpsu4qz	478	4	pH	pH	NNP
cord-268139-tgpsu4qz	478	5	8.0	8.0	CD
cord-268139-tgpsu4qz	478	6	)	)	-RRB-
cord-268139-tgpsu4qz	478	7	.	.	.
cord-268139-tgpsu4qz	479	1	Lysates	Lysates	NNP
cord-268139-tgpsu4qz	479	2	were	be	VBD
cord-268139-tgpsu4qz	479	3	centrifuged	centrifuge	VBN
cord-268139-tgpsu4qz	479	4	at	at	IN
cord-268139-tgpsu4qz	479	5	3500	3500	CD
cord-268139-tgpsu4qz	479	6	Â	â	NN
cord-268139-tgpsu4qz	479	7	g	g	NN
cord-268139-tgpsu4qz	479	8	to	to	TO
cord-268139-tgpsu4qz	479	9	remove	remove	VB
cord-268139-tgpsu4qz	479	10	nuclei	nucleus	NNS
cord-268139-tgpsu4qz	479	11	,	,	,
cord-268139-tgpsu4qz	479	12	and	and	CC
cord-268139-tgpsu4qz	479	13	RNA	RNA	NNP
cord-268139-tgpsu4qz	479	14	in	in	IN
cord-268139-tgpsu4qz	479	15	100	100	CD
cord-268139-tgpsu4qz	479	16	Al	Al	NNP
cord-268139-tgpsu4qz	479	17	of	of	IN
cord-268139-tgpsu4qz	479	18	cytoplasmic	cytoplasmic	JJ
cord-268139-tgpsu4qz	479	19	extract	extract	NN
cord-268139-tgpsu4qz	479	20	was	be	VBD
cord-268139-tgpsu4qz	479	21	precipitated	precipitate	VBN
cord-268139-tgpsu4qz	479	22	in	in	IN
cord-268139-tgpsu4qz	479	23	replicate	replicate	NN
cord-268139-tgpsu4qz	479	24	using	use	VBG
cord-268139-tgpsu4qz	479	25	10	10	CD
cord-268139-tgpsu4qz	479	26	%	%	NN
cord-268139-tgpsu4qz	479	27	trichloroacetic	trichloroacetic	JJ
cord-268139-tgpsu4qz	479	28	acid	acid	NN
cord-268139-tgpsu4qz	479	29	.	.	.
cord-268139-tgpsu4qz	480	1	Precipitated	Precipitated	NNP
cord-268139-tgpsu4qz	480	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	480	3	was	be	VBD
cord-268139-tgpsu4qz	480	4	dried	dry	VBN
cord-268139-tgpsu4qz	480	5	onto	onto	IN
cord-268139-tgpsu4qz	480	6	glass	glass	NN
cord-268139-tgpsu4qz	480	7	microfiber	microfiber	NN
cord-268139-tgpsu4qz	480	8	filters	filter	NNS
cord-268139-tgpsu4qz	480	9	(	(	-LRB-
cord-268139-tgpsu4qz	480	10	Whatman	Whatman	NNP
cord-268139-tgpsu4qz	480	11	)	)	-RRB-
cord-268139-tgpsu4qz	480	12	using	use	VBG
cord-268139-tgpsu4qz	480	13	vacuum	vacuum	NN
cord-268139-tgpsu4qz	480	14	filtration	filtration	NN
cord-268139-tgpsu4qz	480	15	,	,	,
cord-268139-tgpsu4qz	480	16	and	and	CC
cord-268139-tgpsu4qz	480	17	radioactivity	radioactivity	NN
cord-268139-tgpsu4qz	480	18	was	be	VBD
cord-268139-tgpsu4qz	480	19	measured	measure	VBN
cord-268139-tgpsu4qz	480	20	as	as	IN
cord-268139-tgpsu4qz	480	21	counts	count	NNS
cord-268139-tgpsu4qz	480	22	per	per	IN
cord-268139-tgpsu4qz	480	23	minute	minute	NN
cord-268139-tgpsu4qz	480	24	(	(	-LRB-
cord-268139-tgpsu4qz	480	25	CPM	CPM	NNP
cord-268139-tgpsu4qz	480	26	)	)	-RRB-
cord-268139-tgpsu4qz	480	27	using	use	VBG
cord-268139-tgpsu4qz	480	28	a	a	DT
cord-268139-tgpsu4qz	480	29	liquid	liquid	JJ
cord-268139-tgpsu4qz	480	30	scintillation	scintillation	NN
cord-268139-tgpsu4qz	480	31	counter	counter	NN
cord-268139-tgpsu4qz	480	32	(	(	-LRB-
cord-268139-tgpsu4qz	480	33	Beckman	Beckman	NNP
cord-268139-tgpsu4qz	480	34	)	)	-RRB-
cord-268139-tgpsu4qz	480	35	.	.	.
cord-268139-tgpsu4qz	481	1	Statistical	statistical	JJ
cord-268139-tgpsu4qz	481	2	analysis	analysis	NN
cord-268139-tgpsu4qz	481	3	software	software	NN
cord-268139-tgpsu4qz	481	4	(	(	-LRB-
cord-268139-tgpsu4qz	481	5	Smith	Smith	NNP
cord-268139-tgpsu4qz	481	6	's	's	POS
cord-268139-tgpsu4qz	481	7	Statistical	Statistical	NNP
cord-268139-tgpsu4qz	481	8	Package	Package	NNP
cord-268139-tgpsu4qz	481	9	-	-	HYPH
cord-268139-tgpsu4qz	481	10	Version	Version	NNP
cord-268139-tgpsu4qz	481	11	2.75	2.75	CD
cord-268139-tgpsu4qz	481	12	)	)	-RRB-
cord-268139-tgpsu4qz	481	13	was	be	VBD
cord-268139-tgpsu4qz	481	14	used	use	VBN
cord-268139-tgpsu4qz	481	15	for	for	IN
cord-268139-tgpsu4qz	481	16	data	datum	NNS
cord-268139-tgpsu4qz	481	17	analysis	analysis	NN
cord-268139-tgpsu4qz	481	18	.	.	.
cord-268139-tgpsu4qz	482	1	For	for	IN
cord-268139-tgpsu4qz	482	2	each	each	DT
cord-268139-tgpsu4qz	482	3	experiment	experiment	NN
cord-268139-tgpsu4qz	482	4	(	(	-LRB-
cord-268139-tgpsu4qz	482	5	n	n	NN
cord-268139-tgpsu4qz	482	6	=	=	SYM
cord-268139-tgpsu4qz	482	7	5	5	CD
cord-268139-tgpsu4qz	482	8	)	)	-RRB-
cord-268139-tgpsu4qz	482	9	,	,	,
cord-268139-tgpsu4qz	482	10	icwt	icwt	JJ
cord-268139-tgpsu4qz	482	11	values	value	NNS
cord-268139-tgpsu4qz	482	12	(	(	-LRB-
cord-268139-tgpsu4qz	482	13	CPM	CPM	NNP
cord-268139-tgpsu4qz	482	14	)	)	-RRB-
cord-268139-tgpsu4qz	482	15	from	from	IN
cord-268139-tgpsu4qz	482	16	the	the	DT
cord-268139-tgpsu4qz	482	17	5	5	CD
cord-268139-tgpsu4qz	482	18	-	-	SYM
cord-268139-tgpsu4qz	482	19	9	9	CD
cord-268139-tgpsu4qz	482	20	h	h	NN
cord-268139-tgpsu4qz	482	21	time	time	NN
cord-268139-tgpsu4qz	482	22	intervals	interval	NNS
cord-268139-tgpsu4qz	482	23	were	be	VBD
cord-268139-tgpsu4qz	482	24	set	set	VBN
cord-268139-tgpsu4qz	482	25	to	to	TO
cord-268139-tgpsu4qz	482	26	equal	equal	VB
cord-268139-tgpsu4qz	482	27	100	100	CD
cord-268139-tgpsu4qz	482	28	%	%	NN
cord-268139-tgpsu4qz	482	29	.	.	.
cord-268139-tgpsu4qz	483	1	And	and	CC
cord-268139-tgpsu4qz	483	2	,	,	,
cord-268139-tgpsu4qz	483	3	nsp1	nsp1	VBZ
cord-268139-tgpsu4qz	483	4	mutant	mutant	JJ
cord-268139-tgpsu4qz	483	5	values	value	NNS
cord-268139-tgpsu4qz	483	6	were	be	VBD
cord-268139-tgpsu4qz	483	7	calculated	calculate	VBN
cord-268139-tgpsu4qz	483	8	as	as	IN
cord-268139-tgpsu4qz	483	9	percent	percent	NN
cord-268139-tgpsu4qz	483	10	of	of	IN
cord-268139-tgpsu4qz	483	11	icwt	icwt	NNP
cord-268139-tgpsu4qz	483	12	.	.	.
cord-268139-tgpsu4qz	484	1	One	one	CD
cord-268139-tgpsu4qz	484	2	-	-	HYPH
cord-268139-tgpsu4qz	484	3	sample	sample	NN
cord-268139-tgpsu4qz	484	4	t	t	NN
cord-268139-tgpsu4qz	484	5	tests	test	NNS
cord-268139-tgpsu4qz	484	6	were	be	VBD
cord-268139-tgpsu4qz	484	7	performed	perform	VBN
cord-268139-tgpsu4qz	484	8	using	use	VBG
cord-268139-tgpsu4qz	484	9	a	a	DT
cord-268139-tgpsu4qz	484	10	P	p	NN
cord-268139-tgpsu4qz	484	11	value	value	NN
cord-268139-tgpsu4qz	484	12	0.05	0.05	CD
cord-268139-tgpsu4qz	484	13	.	.	.
cord-268139-tgpsu4qz	485	1	Analysis	analysis	NN
cord-268139-tgpsu4qz	485	2	of	of	IN
cord-268139-tgpsu4qz	485	3	[	[	-LRB-
cord-268139-tgpsu4qz	485	4	3	3	CD
cord-268139-tgpsu4qz	485	5	H	H	NNP
cord-268139-tgpsu4qz	485	6	]	]	-RRB-
cord-268139-tgpsu4qz	485	7	viral	viral	JJ
cord-268139-tgpsu4qz	485	8	RNA	rna	NN
cord-268139-tgpsu4qz	485	9	by	by	IN
cord-268139-tgpsu4qz	485	10	gel	gel	NN
cord-268139-tgpsu4qz	485	11	electrophoresis	electrophoresis	NN
cord-268139-tgpsu4qz	485	12	was	be	VBD
cord-268139-tgpsu4qz	485	13	performed	perform	VBN
cord-268139-tgpsu4qz	485	14	as	as	IN
cord-268139-tgpsu4qz	485	15	described	describe	VBN
cord-268139-tgpsu4qz	485	16	previously	previously	RB
cord-268139-tgpsu4qz	485	17	(	(	-LRB-
cord-268139-tgpsu4qz	485	18	Kim	Kim	NNP
cord-268139-tgpsu4qz	485	19	et	et	NNP
cord-268139-tgpsu4qz	485	20	al	al	NNP
cord-268139-tgpsu4qz	485	21	.	.	NNP
cord-268139-tgpsu4qz	485	22	,	,	,
cord-268139-tgpsu4qz	485	23	1995	1995	CD
cord-268139-tgpsu4qz	485	24	)	)	-RRB-
cord-268139-tgpsu4qz	485	25	with	with	IN
cord-268139-tgpsu4qz	485	26	a	a	DT
cord-268139-tgpsu4qz	485	27	few	few	JJ
cord-268139-tgpsu4qz	485	28	modifications	modification	NNS
cord-268139-tgpsu4qz	485	29	.	.	.
cord-268139-tgpsu4qz	486	1	Viral	Viral	NNP
cord-268139-tgpsu4qz	486	2	RNA	RNA	NNP
cord-268139-tgpsu4qz	486	3	was	be	VBD
cord-268139-tgpsu4qz	486	4	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	486	5	from	from	IN
cord-268139-tgpsu4qz	486	6	5	5	CD
cord-268139-tgpsu4qz	486	7	-	-	SYM
cord-268139-tgpsu4qz	486	8	9	9	CD
cord-268139-tgpsu4qz	486	9	h	h	NN
cord-268139-tgpsu4qz	486	10	p.i	p.i	NNP
cord-268139-tgpsu4qz	486	11	.	.	NNP
cord-268139-tgpsu4qz	486	12	as	as	IN
cord-268139-tgpsu4qz	486	13	before	before	RB
cord-268139-tgpsu4qz	486	14	and	and	CC
cord-268139-tgpsu4qz	486	15	was	be	VBD
cord-268139-tgpsu4qz	486	16	isolated	isolate	VBN
cord-268139-tgpsu4qz	486	17	using	use	VBG
cord-268139-tgpsu4qz	486	18	Trizol	Trizol	NNP
cord-268139-tgpsu4qz	486	19	(	(	-LRB-
cord-268139-tgpsu4qz	486	20	Invitrogen	Invitrogen	NNP
cord-268139-tgpsu4qz	486	21	)	)	-RRB-
cord-268139-tgpsu4qz	486	22	according	accord	VBG
cord-268139-tgpsu4qz	486	23	to	to	IN
cord-268139-tgpsu4qz	486	24	the	the	DT
cord-268139-tgpsu4qz	486	25	manufacturer	manufacturer	NN
cord-268139-tgpsu4qz	486	26	's	's	POS
cord-268139-tgpsu4qz	486	27	protocol	protocol	NN
cord-268139-tgpsu4qz	486	28	.	.	.
cord-268139-tgpsu4qz	487	1	The	the	DT
cord-268139-tgpsu4qz	487	2	amount	amount	NN
cord-268139-tgpsu4qz	487	3	of	of	IN
cord-268139-tgpsu4qz	487	4	[	[	-LRB-
cord-268139-tgpsu4qz	487	5	3	3	CD
cord-268139-tgpsu4qz	487	6	H	h	NN
cord-268139-tgpsu4qz	487	7	]	]	-RRB-
cord-268139-tgpsu4qz	487	8	incorporation	incorporation	NN
cord-268139-tgpsu4qz	487	9	was	be	VBD
cord-268139-tgpsu4qz	487	10	quantitated	quantitate	VBN
cord-268139-tgpsu4qz	487	11	using	use	VBG
cord-268139-tgpsu4qz	487	12	liquid	liquid	JJ
cord-268139-tgpsu4qz	487	13	scintillation	scintillation	NN
cord-268139-tgpsu4qz	487	14	.	.	.
cord-268139-tgpsu4qz	488	1	Equal	equal	JJ
cord-268139-tgpsu4qz	488	2	amounts	amount	NNS
cord-268139-tgpsu4qz	488	3	of	of	IN
cord-268139-tgpsu4qz	488	4	radiolabeled	radiolabele	VBN
cord-268139-tgpsu4qz	488	5	viral	viral	JJ
cord-268139-tgpsu4qz	488	6	RNA	RNA	NNP
cord-268139-tgpsu4qz	488	7	(	(	-LRB-
cord-268139-tgpsu4qz	488	8	approximately	approximately	RB
cord-268139-tgpsu4qz	488	9	400,000	400,000	CD
cord-268139-tgpsu4qz	488	10	CPM	CPM	NNS
cord-268139-tgpsu4qz	488	11	from	from	IN
cord-268139-tgpsu4qz	488	12	a	a	DT
cord-268139-tgpsu4qz	488	13	maximum	maximum	NN
cord-268139-tgpsu4qz	488	14	of	of	IN
cord-268139-tgpsu4qz	488	15	10	10	CD
cord-268139-tgpsu4qz	488	16	6	6	CD
cord-268139-tgpsu4qz	488	17	cells	cell	NNS
cord-268139-tgpsu4qz	488	18	)	)	-RRB-
cord-268139-tgpsu4qz	488	19	were	be	VBD
cord-268139-tgpsu4qz	488	20	denatured	denature	VBN
cord-268139-tgpsu4qz	488	21	in	in	IN
cord-268139-tgpsu4qz	488	22	formamide	formamide	JJ
cord-268139-tgpsu4qz	488	23	gel	gel	NN
cord-268139-tgpsu4qz	488	24	loading	loading	NN
cord-268139-tgpsu4qz	488	25	buffer	buffer	NN
cord-268139-tgpsu4qz	488	26	at	at	IN
cord-268139-tgpsu4qz	488	27	65	65	CD
cord-268139-tgpsu4qz	488	28	-C	-C	NNS
cord-268139-tgpsu4qz	488	29	for	for	IN
cord-268139-tgpsu4qz	488	30	10	10	CD
cord-268139-tgpsu4qz	488	31	min	min	NN
cord-268139-tgpsu4qz	488	32	and	and	CC
cord-268139-tgpsu4qz	488	33	then	then	RB
cord-268139-tgpsu4qz	488	34	electrophoresed	electrophorese	VBD
cord-268139-tgpsu4qz	488	35	in	in	IN
cord-268139-tgpsu4qz	488	36	a	a	DT
cord-268139-tgpsu4qz	488	37	0.8	0.8	CD
cord-268139-tgpsu4qz	488	38	%	%	NN
cord-268139-tgpsu4qz	488	39	formaldehyde/	formaldehyde/	CD
cord-268139-tgpsu4qz	488	40	agarose	agarose	NN
cord-268139-tgpsu4qz	488	41	gel	gel	NN
cord-268139-tgpsu4qz	488	42	at	at	IN
cord-268139-tgpsu4qz	488	43	144	144	CD
cord-268139-tgpsu4qz	488	44	V	v	NN
cord-268139-tgpsu4qz	488	45	for	for	IN
cord-268139-tgpsu4qz	488	46	4	4	CD
cord-268139-tgpsu4qz	488	47	h.	h.	NN
cord-268139-tgpsu4qz	489	1	For	for	IN
cord-268139-tgpsu4qz	489	2	mock	mock	JJ
cord-268139-tgpsu4qz	489	3	-	-	HYPH
cord-268139-tgpsu4qz	489	4	infected	infect	VBN
cord-268139-tgpsu4qz	489	5	samples	sample	NNS
cord-268139-tgpsu4qz	489	6	,	,	,
cord-268139-tgpsu4qz	489	7	RNA	RNA	NNP
cord-268139-tgpsu4qz	489	8	from	from	IN
cord-268139-tgpsu4qz	489	9	10	10	CD
cord-268139-tgpsu4qz	489	10	6	6	CD
cord-268139-tgpsu4qz	489	11	cells	cell	NNS
cord-268139-tgpsu4qz	489	12	was	be	VBD
cord-268139-tgpsu4qz	489	13	used	use	VBN
cord-268139-tgpsu4qz	489	14	.	.	.
cord-268139-tgpsu4qz	490	1	Following	follow	VBG
cord-268139-tgpsu4qz	490	2	electrophoresis	electrophoresis	NN
cord-268139-tgpsu4qz	490	3	,	,	,
cord-268139-tgpsu4qz	490	4	the	the	DT
cord-268139-tgpsu4qz	490	5	gel	gel	NN
cord-268139-tgpsu4qz	490	6	was	be	VBD
cord-268139-tgpsu4qz	490	7	incubated	incubate	VBN
cord-268139-tgpsu4qz	490	8	in	in	IN
cord-268139-tgpsu4qz	490	9	100	100	CD
cord-268139-tgpsu4qz	490	10	%	%	NN
cord-268139-tgpsu4qz	490	11	methanol	methanol	NN
cord-268139-tgpsu4qz	490	12	for	for	IN
cord-268139-tgpsu4qz	490	13	2	2	CD
cord-268139-tgpsu4qz	490	14	h	h	NN
cord-268139-tgpsu4qz	490	15	then	then	RB
cord-268139-tgpsu4qz	490	16	in	in	IN
cord-268139-tgpsu4qz	490	17	1	1	CD
cord-268139-tgpsu4qz	490	18	%	%	NN
cord-268139-tgpsu4qz	490	19	diphenyloxazole	diphenyloxazole	NN
cord-268139-tgpsu4qz	490	20	/	/	SYM
cord-268139-tgpsu4qz	490	21	methanol	methanol	NN
cord-268139-tgpsu4qz	490	22	for	for	IN
cord-268139-tgpsu4qz	490	23	2	2	CD
cord-268139-tgpsu4qz	490	24	h	h	NN
cord-268139-tgpsu4qz	490	25	prior	prior	RB
cord-268139-tgpsu4qz	490	26	to	to	IN
cord-268139-tgpsu4qz	490	27	overnight	overnight	JJ
cord-268139-tgpsu4qz	490	28	incubation	incubation	NN
cord-268139-tgpsu4qz	490	29	in	in	IN
cord-268139-tgpsu4qz	490	30	distilled	distilled	JJ
cord-268139-tgpsu4qz	490	31	water	water	NN
cord-268139-tgpsu4qz	490	32	.	.	.
cord-268139-tgpsu4qz	491	1	The	the	DT
cord-268139-tgpsu4qz	491	2	gel	gel	NN
cord-268139-tgpsu4qz	491	3	was	be	VBD
cord-268139-tgpsu4qz	491	4	dried	dry	VBN
cord-268139-tgpsu4qz	491	5	at	at	IN
cord-268139-tgpsu4qz	491	6	50	50	CD
cord-268139-tgpsu4qz	491	7	-C	-C	NNS
cord-268139-tgpsu4qz	491	8	using	use	VBG
cord-268139-tgpsu4qz	491	9	vacuum	vacuum	NN
cord-268139-tgpsu4qz	491	10	filtration	filtration	NN
cord-268139-tgpsu4qz	491	11	before	before	IN
cord-268139-tgpsu4qz	491	12	exposing	expose	VBG
cord-268139-tgpsu4qz	491	13	it	-PRON-	PRP
cord-268139-tgpsu4qz	491	14	to	to	TO
cord-268139-tgpsu4qz	491	15	film	film	VB
cord-268139-tgpsu4qz	491	16	.	.	.
cord-268139-tgpsu4qz	492	1	Images	image	NNS
cord-268139-tgpsu4qz	492	2	were	be	VBD
cord-268139-tgpsu4qz	492	3	prepared	prepare	VBN
cord-268139-tgpsu4qz	492	4	using	use	VBG
cord-268139-tgpsu4qz	492	5	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	492	6	Photoshop	Photoshop	NNP
cord-268139-tgpsu4qz	492	7	8.0	8.0	CD
cord-268139-tgpsu4qz	492	8	(	(	-LRB-
cord-268139-tgpsu4qz	492	9	Adobe	Adobe	NNP
cord-268139-tgpsu4qz	492	10	)	)	-RRB-
cord-268139-tgpsu4qz	492	11	.	.	.
cord-268139-tgpsu4qz	493	1	Efficient	efficient	JJ
cord-268139-tgpsu4qz	493	2	rescue	rescue	NN
cord-268139-tgpsu4qz	493	3	of	of	IN
cord-268139-tgpsu4qz	493	4	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	493	5	C	C	NNP
cord-268139-tgpsu4qz	493	6	virus	virus	NN
cord-268139-tgpsu4qz	493	7	RNA	RNA	NNP
cord-268139-tgpsu4qz	493	8	replication	replication	NN
cord-268139-tgpsu4qz	493	9	by	by	IN
cord-268139-tgpsu4qz	493	10	trans	trans	NN
cord-268139-tgpsu4qz	493	11	-	-	NN
cord-268139-tgpsu4qz	493	12	complementation	complementation	NN
cord-268139-tgpsu4qz	493	13	with	with	IN
cord-268139-tgpsu4qz	493	14	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	493	15	protein	protein	NN
cord-268139-tgpsu4qz	493	16	5A	5a	NN
cord-268139-tgpsu4qz	493	17	Identification	identification	NN
cord-268139-tgpsu4qz	493	18	of	of	IN
cord-268139-tgpsu4qz	493	19	a	a	DT
cord-268139-tgpsu4qz	493	20	domain	domain	NN
cord-268139-tgpsu4qz	493	21	required	require	VBN
cord-268139-tgpsu4qz	493	22	for	for	IN
cord-268139-tgpsu4qz	493	23	autoproteolytic	autoproteolytic	JJ
cord-268139-tgpsu4qz	493	24	cleavage	cleavage	NN
cord-268139-tgpsu4qz	493	25	of	of	IN
cord-268139-tgpsu4qz	493	26	murine	murine	JJ
cord-268139-tgpsu4qz	493	27	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	493	28	gene	gene	NN
cord-268139-tgpsu4qz	493	29	A	A	NNP
cord-268139-tgpsu4qz	493	30	polyprotein	polyprotein	JJ
cord-268139-tgpsu4qz	493	31	Identification	identification	NN
cord-268139-tgpsu4qz	493	32	of	of	IN
cord-268139-tgpsu4qz	493	33	the	the	DT
cord-268139-tgpsu4qz	493	34	catalytic	catalytic	JJ
cord-268139-tgpsu4qz	493	35	sites	site	NNS
cord-268139-tgpsu4qz	493	36	of	of	IN
cord-268139-tgpsu4qz	493	37	a	a	DT
cord-268139-tgpsu4qz	493	38	papain	papain	NN
cord-268139-tgpsu4qz	493	39	-	-	HYPH
cord-268139-tgpsu4qz	493	40	like	like	JJ
cord-268139-tgpsu4qz	493	41	cysteine	cysteine	NN
cord-268139-tgpsu4qz	493	42	proteinase	proteinase	NN
cord-268139-tgpsu4qz	493	43	of	of	IN
cord-268139-tgpsu4qz	493	44	murine	murine	JJ
cord-268139-tgpsu4qz	493	45	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	494	1	The	the	DT
cord-268139-tgpsu4qz	494	2	severe	severe	JJ
cord-268139-tgpsu4qz	494	3	acute	acute	JJ
cord-268139-tgpsu4qz	494	4	respiratory	respiratory	JJ
cord-268139-tgpsu4qz	494	5	syndrome	syndrome	NN
cord-268139-tgpsu4qz	494	6	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	495	1	Nsp15	Nsp15	NNP
cord-268139-tgpsu4qz	495	2	protein	protein	NN
cord-268139-tgpsu4qz	495	3	is	be	VBZ
cord-268139-tgpsu4qz	495	4	an	an	DT
cord-268139-tgpsu4qz	495	5	endoribonuclease	endoribonuclease	NN
cord-268139-tgpsu4qz	495	6	that	that	WDT
cord-268139-tgpsu4qz	495	7	prefers	prefer	VBZ
cord-268139-tgpsu4qz	495	8	manganese	manganese	NN
cord-268139-tgpsu4qz	495	9	as	as	IN
cord-268139-tgpsu4qz	495	10	a	a	DT
cord-268139-tgpsu4qz	495	11	cofactor	cofactor	NN
cord-268139-tgpsu4qz	495	12	Mouse	Mouse	NNP
cord-268139-tgpsu4qz	495	13	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	495	14	virus	virus	NN
cord-268139-tgpsu4qz	495	15	strain	strain	VBP
cord-268139-tgpsu4qz	495	16	A59	A59	NNP
cord-268139-tgpsu4qz	495	17	RNA	RNA	NNP
cord-268139-tgpsu4qz	495	18	polymerase	polymerase	NN
cord-268139-tgpsu4qz	495	19	gene	gene	NN
cord-268139-tgpsu4qz	495	20	ORF	ORF	NNP
cord-268139-tgpsu4qz	495	21	1a	1a	NN
cord-268139-tgpsu4qz	495	22	:	:	:
cord-268139-tgpsu4qz	496	1	heterogeneity	heterogeneity	NN
cord-268139-tgpsu4qz	496	2	among	among	IN
cord-268139-tgpsu4qz	496	3	MHV	MHV	NNP
cord-268139-tgpsu4qz	496	4	strains	strain	VBZ
cord-268139-tgpsu4qz	496	5	Characterization	characterization	NN
cord-268139-tgpsu4qz	496	6	of	of	IN
cord-268139-tgpsu4qz	496	7	the	the	DT
cord-268139-tgpsu4qz	496	8	leader	leader	NN
cord-268139-tgpsu4qz	496	9	papain	papain	NN
cord-268139-tgpsu4qz	496	10	-	-	HYPH
cord-268139-tgpsu4qz	496	11	like	like	JJ
cord-268139-tgpsu4qz	496	12	proteinase	proteinase	NN
cord-268139-tgpsu4qz	496	13	of	of	IN
cord-268139-tgpsu4qz	496	14	MHV	MHV	NNP
cord-268139-tgpsu4qz	496	15	-	-	HYPH
cord-268139-tgpsu4qz	496	16	A59	a59	NN
cord-268139-tgpsu4qz	496	17	:	:	:
cord-268139-tgpsu4qz	496	18	identification	identification	NN
cord-268139-tgpsu4qz	496	19	of	of	IN
cord-268139-tgpsu4qz	496	20	a	a	DT
cord-268139-tgpsu4qz	496	21	new	new	JJ
cord-268139-tgpsu4qz	496	22	in	in	FW
cord-268139-tgpsu4qz	496	23	vitro	vitro	FW
cord-268139-tgpsu4qz	496	24	cleavage	cleavage	NN
cord-268139-tgpsu4qz	496	25	site	site	NN
cord-268139-tgpsu4qz	496	26	Four	four	CD
cord-268139-tgpsu4qz	496	27	proteins	protein	NNS
cord-268139-tgpsu4qz	496	28	processed	process	VBN
cord-268139-tgpsu4qz	496	29	from	from	IN
cord-268139-tgpsu4qz	496	30	the	the	DT
cord-268139-tgpsu4qz	496	31	replicase	replicase	NNP
cord-268139-tgpsu4qz	496	32	gene	gene	NNP
cord-268139-tgpsu4qz	496	33	polyprotein	polyprotein	NNP
cord-268139-tgpsu4qz	496	34	of	of	IN
cord-268139-tgpsu4qz	496	35	mouse	mouse	NNP
cord-268139-tgpsu4qz	496	36	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	496	37	virus	virus	NN
cord-268139-tgpsu4qz	496	38	colocalize	colocalize	NN
cord-268139-tgpsu4qz	496	39	in	in	IN
cord-268139-tgpsu4qz	496	40	the	the	DT
cord-268139-tgpsu4qz	496	41	cell	cell	NN
cord-268139-tgpsu4qz	496	42	periphery	periphery	NN
cord-268139-tgpsu4qz	496	43	and	and	CC
cord-268139-tgpsu4qz	496	44	adjacent	adjacent	JJ
cord-268139-tgpsu4qz	496	45	to	to	IN
cord-268139-tgpsu4qz	496	46	sites	site	NNS
cord-268139-tgpsu4qz	496	47	of	of	IN
cord-268139-tgpsu4qz	496	48	virion	virion	NN
cord-268139-tgpsu4qz	496	49	assembly	assembly	NN
cord-268139-tgpsu4qz	496	50	Mouse	Mouse	NNP
cord-268139-tgpsu4qz	496	51	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	496	52	virus	virus	NN
cord-268139-tgpsu4qz	496	53	replicase	replicase	NNP
cord-268139-tgpsu4qz	496	54	protein	protein	NN
cord-268139-tgpsu4qz	496	55	complexes	complex	NNS
cord-268139-tgpsu4qz	496	56	are	be	VBP
cord-268139-tgpsu4qz	496	57	translocated	translocate	VBN
cord-268139-tgpsu4qz	496	58	to	to	IN
cord-268139-tgpsu4qz	496	59	sites	site	NNS
cord-268139-tgpsu4qz	496	60	of	of	IN
cord-268139-tgpsu4qz	496	61	M	M	NNP
cord-268139-tgpsu4qz	496	62	protein	protein	NN
cord-268139-tgpsu4qz	496	63	accumulation	accumulation	NN
cord-268139-tgpsu4qz	496	64	in	in	IN
cord-268139-tgpsu4qz	496	65	the	the	DT
cord-268139-tgpsu4qz	496	66	ERGIC	ERGIC	NNP
cord-268139-tgpsu4qz	496	67	at	at	IN
cord-268139-tgpsu4qz	496	68	late	late	JJ
cord-268139-tgpsu4qz	496	69	times	time	NNS
cord-268139-tgpsu4qz	496	70	of	of	IN
cord-268139-tgpsu4qz	496	71	infection	infection	NN
cord-268139-tgpsu4qz	497	1	The	the	DT
cord-268139-tgpsu4qz	497	2	primary	primary	JJ
cord-268139-tgpsu4qz	497	3	structure	structure	NN
cord-268139-tgpsu4qz	497	4	and	and	CC
cord-268139-tgpsu4qz	497	5	expression	expression	NN
cord-268139-tgpsu4qz	497	6	of	of	IN
cord-268139-tgpsu4qz	497	7	the	the	DT
cord-268139-tgpsu4qz	497	8	second	second	JJ
cord-268139-tgpsu4qz	497	9	open	open	JJ
cord-268139-tgpsu4qz	497	10	reading	reading	NN
cord-268139-tgpsu4qz	497	11	frame	frame	NN
cord-268139-tgpsu4qz	497	12	of	of	IN
cord-268139-tgpsu4qz	497	13	the	the	DT
cord-268139-tgpsu4qz	497	14	polymerase	polymerase	NN
cord-268139-tgpsu4qz	497	15	gene	gene	NN
cord-268139-tgpsu4qz	497	16	of	of	IN
cord-268139-tgpsu4qz	497	17	the	the	DT
cord-268139-tgpsu4qz	497	18	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	497	19	MHV	MHV	NNP
cord-268139-tgpsu4qz	497	20	-	-	HYPH
cord-268139-tgpsu4qz	497	21	A59	A59	NNP
cord-268139-tgpsu4qz	497	22	;	;	:
cord-268139-tgpsu4qz	497	23	a	a	DT
cord-268139-tgpsu4qz	497	24	highly	highly	RB
cord-268139-tgpsu4qz	497	25	conserved	conserved	JJ
cord-268139-tgpsu4qz	497	26	polymerase	polymerase	NN
cord-268139-tgpsu4qz	497	27	is	be	VBZ
cord-268139-tgpsu4qz	497	28	expressed	express	VBN
cord-268139-tgpsu4qz	497	29	by	by	IN
cord-268139-tgpsu4qz	497	30	an	an	DT
cord-268139-tgpsu4qz	497	31	efficient	efficient	JJ
cord-268139-tgpsu4qz	497	32	ribosomal	ribosomal	JJ
cord-268139-tgpsu4qz	497	33	frameshifting	frameshifting	NN
cord-268139-tgpsu4qz	497	34	mechanism	mechanism	NN
cord-268139-tgpsu4qz	497	35	Characterization	characterization	NN
cord-268139-tgpsu4qz	497	36	of	of	IN
cord-268139-tgpsu4qz	497	37	an	an	DT
cord-268139-tgpsu4qz	497	38	efficient	efficient	JJ
cord-268139-tgpsu4qz	497	39	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	497	40	ribosomal	ribosomal	NN
cord-268139-tgpsu4qz	497	41	frameshifting	frameshifte	VBG
cord-268139-tgpsu4qz	497	42	signal	signal	NN
cord-268139-tgpsu4qz	497	43	:	:	:
cord-268139-tgpsu4qz	497	44	requirement	requirement	NN
cord-268139-tgpsu4qz	497	45	for	for	IN
cord-268139-tgpsu4qz	497	46	an	an	DT
cord-268139-tgpsu4qz	497	47	RNA	RNA	NNP
cord-268139-tgpsu4qz	497	48	pseudoknot	pseudoknot	NN
cord-268139-tgpsu4qz	497	49	Characterization	characterization	NN
cord-268139-tgpsu4qz	497	50	of	of	IN
cord-268139-tgpsu4qz	497	51	the	the	DT
cord-268139-tgpsu4qz	497	52	expression	expression	NN
cord-268139-tgpsu4qz	497	53	,	,	,
cord-268139-tgpsu4qz	497	54	intracellular	intracellular	JJ
cord-268139-tgpsu4qz	497	55	localization	localization	NN
cord-268139-tgpsu4qz	497	56	,	,	,
cord-268139-tgpsu4qz	497	57	and	and	CC
cord-268139-tgpsu4qz	497	58	replication	replication	NN
cord-268139-tgpsu4qz	497	59	complex	complex	NNP
cord-268139-tgpsu4qz	497	60	association	association	NNP
cord-268139-tgpsu4qz	497	61	of	of	IN
cord-268139-tgpsu4qz	497	62	the	the	DT
cord-268139-tgpsu4qz	497	63	putative	putative	JJ
cord-268139-tgpsu4qz	497	64	mouse	mouse	NN
cord-268139-tgpsu4qz	497	65	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	497	66	virus	virus	NN
cord-268139-tgpsu4qz	497	67	RNAdependent	RNAdependent	NNP
cord-268139-tgpsu4qz	497	68	RNA	RNA	NNP
cord-268139-tgpsu4qz	497	69	polymerase	polymerase	NN
cord-268139-tgpsu4qz	498	1	Intracellular	Intracellular	NNP
cord-268139-tgpsu4qz	498	2	localization	localization	NN
cord-268139-tgpsu4qz	498	3	and	and	CC
cord-268139-tgpsu4qz	498	4	protein	protein	NN
cord-268139-tgpsu4qz	498	5	interactions	interaction	NNS
cord-268139-tgpsu4qz	498	6	of	of	IN
cord-268139-tgpsu4qz	498	7	the	the	DT
cord-268139-tgpsu4qz	498	8	gene	gene	NN
cord-268139-tgpsu4qz	498	9	1	1	CD
cord-268139-tgpsu4qz	498	10	protein	protein	NN
cord-268139-tgpsu4qz	498	11	p28	p28	NN
cord-268139-tgpsu4qz	498	12	during	during	IN
cord-268139-tgpsu4qz	498	13	mouse	mouse	NNP
cord-268139-tgpsu4qz	498	14	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	498	15	virus	virus	NN
cord-268139-tgpsu4qz	498	16	replication	replication	NN
cord-268139-tgpsu4qz	498	17	Human	Human	NNP
cord-268139-tgpsu4qz	498	18	carcinoembryonic	carcinoembryonic	JJ
cord-268139-tgpsu4qz	498	19	antigen	antigen	NN
cord-268139-tgpsu4qz	498	20	and	and	CC
cord-268139-tgpsu4qz	498	21	biliary	biliary	JJ
cord-268139-tgpsu4qz	498	22	glycoprotein	glycoprotein	NN
cord-268139-tgpsu4qz	498	23	can	can	MD
cord-268139-tgpsu4qz	498	24	serve	serve	VB
cord-268139-tgpsu4qz	498	25	as	as	IN
cord-268139-tgpsu4qz	498	26	mouse	mouse	NN
cord-268139-tgpsu4qz	498	27	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	498	28	virus	virus	NN
cord-268139-tgpsu4qz	498	29	receptors	receptor	NNS
cord-268139-tgpsu4qz	498	30	Murine	murine	JJ
cord-268139-tgpsu4qz	498	31	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	498	32	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	498	33	protein	protein	NN
cord-268139-tgpsu4qz	498	34	p28	p28	CD
cord-268139-tgpsu4qz	498	35	arrests	arrest	NNS
cord-268139-tgpsu4qz	498	36	cell	cell	NN
cord-268139-tgpsu4qz	498	37	cycle	cycle	NN
cord-268139-tgpsu4qz	498	38	in	in	IN
cord-268139-tgpsu4qz	498	39	G0	G0	NNP
cord-268139-tgpsu4qz	498	40	/	/	SYM
cord-268139-tgpsu4qz	498	41	G1	G1	NNP
cord-268139-tgpsu4qz	498	42	phase	phase	NN
cord-268139-tgpsu4qz	498	43	Expression	expression	NN
cord-268139-tgpsu4qz	498	44	,	,	,
cord-268139-tgpsu4qz	498	45	purification	purification	NN
cord-268139-tgpsu4qz	498	46	,	,	,
cord-268139-tgpsu4qz	498	47	and	and	CC
cord-268139-tgpsu4qz	498	48	characterization	characterization	NN
cord-268139-tgpsu4qz	498	49	of	of	IN
cord-268139-tgpsu4qz	498	50	SARS	SARS	NNP
cord-268139-tgpsu4qz	498	51	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	498	52	RNA	RNA	NNP
cord-268139-tgpsu4qz	498	53	polymerase	polymerase	NN
cord-268139-tgpsu4qz	498	54	High	high	JJ
cord-268139-tgpsu4qz	498	55	-	-	HYPH
cord-268139-tgpsu4qz	498	56	resolution	resolution	NN
cord-268139-tgpsu4qz	498	57	epitope	epitope	NN
cord-268139-tgpsu4qz	498	58	mapping	mapping	NN
cord-268139-tgpsu4qz	498	59	of	of	IN
cord-268139-tgpsu4qz	498	60	hGH	hGH	NNP
cord-268139-tgpsu4qz	498	61	-receptor	-receptor	NN
cord-268139-tgpsu4qz	498	62	interactions	interaction	NNS
cord-268139-tgpsu4qz	498	63	by	by	IN
cord-268139-tgpsu4qz	498	64	alanine	alanine	NN
cord-268139-tgpsu4qz	498	65	-	-	HYPH
cord-268139-tgpsu4qz	498	66	scanning	scan	VBG
cord-268139-tgpsu4qz	498	67	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	498	68	Translation	translation	NN
cord-268139-tgpsu4qz	498	69	and	and	CC
cord-268139-tgpsu4qz	498	70	processing	processing	NN
cord-268139-tgpsu4qz	498	71	of	of	IN
cord-268139-tgpsu4qz	498	72	mouse	mouse	NN
cord-268139-tgpsu4qz	498	73	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	498	74	virus	virus	NN
cord-268139-tgpsu4qz	498	75	virion	virion	NN
cord-268139-tgpsu4qz	498	76	RNA	RNA	NNP
cord-268139-tgpsu4qz	498	77	in	in	IN
cord-268139-tgpsu4qz	498	78	a	a	DT
cord-268139-tgpsu4qz	498	79	cell	cell	NN
cord-268139-tgpsu4qz	498	80	-	-	HYPH
cord-268139-tgpsu4qz	498	81	free	free	JJ
cord-268139-tgpsu4qz	498	82	system	system	NN
cord-268139-tgpsu4qz	498	83	Identification	identification	NN
cord-268139-tgpsu4qz	498	84	of	of	IN
cord-268139-tgpsu4qz	498	85	putative	putative	JJ
cord-268139-tgpsu4qz	498	86	polymerase	polymerase	NN
cord-268139-tgpsu4qz	498	87	gene	gene	NN
cord-268139-tgpsu4qz	498	88	product	product	NN
cord-268139-tgpsu4qz	498	89	in	in	IN
cord-268139-tgpsu4qz	498	90	cells	cell	NNS
cord-268139-tgpsu4qz	498	91	infected	infect	VBN
cord-268139-tgpsu4qz	498	92	with	with	IN
cord-268139-tgpsu4qz	498	93	murine	murine	JJ
cord-268139-tgpsu4qz	498	94	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	498	95	A59	A59	NNP
cord-268139-tgpsu4qz	498	96	Intracellular	Intracellular	NNP
cord-268139-tgpsu4qz	498	97	processing	processing	NN
cord-268139-tgpsu4qz	498	98	of	of	IN
cord-268139-tgpsu4qz	498	99	the	the	DT
cord-268139-tgpsu4qz	498	100	N	n	CD
cord-268139-tgpsu4qz	498	101	-	-	HYPH
cord-268139-tgpsu4qz	498	102	terminal	terminal	JJ
cord-268139-tgpsu4qz	498	103	ORF	ORF	NNP
cord-268139-tgpsu4qz	498	104	1a	1a	CD
cord-268139-tgpsu4qz	498	105	proteins	protein	NNS
cord-268139-tgpsu4qz	498	106	of	of	IN
cord-268139-tgpsu4qz	498	107	the	the	DT
cord-268139-tgpsu4qz	498	108	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	498	109	MHV	MHV	NNP
cord-268139-tgpsu4qz	498	110	-	-	HYPH
cord-268139-tgpsu4qz	498	111	A59	A59	NNP
cord-268139-tgpsu4qz	498	112	requires	require	VBZ
cord-268139-tgpsu4qz	498	113	multiple	multiple	JJ
cord-268139-tgpsu4qz	498	114	proteolytic	proteolytic	JJ
cord-268139-tgpsu4qz	498	115	events	event	NNS
cord-268139-tgpsu4qz	498	116	Identification	identification	NN
cord-268139-tgpsu4qz	498	117	and	and	CC
cord-268139-tgpsu4qz	498	118	characterization	characterization	NN
cord-268139-tgpsu4qz	498	119	of	of	IN
cord-268139-tgpsu4qz	498	120	a	a	DT
cord-268139-tgpsu4qz	498	121	65-kDa	65-kda	CD
cord-268139-tgpsu4qz	498	122	protein	protein	NN
cord-268139-tgpsu4qz	498	123	processed	process	VBN
cord-268139-tgpsu4qz	498	124	from	from	IN
cord-268139-tgpsu4qz	498	125	the	the	DT
cord-268139-tgpsu4qz	498	126	gene	gene	NN
cord-268139-tgpsu4qz	498	127	1	1	CD
cord-268139-tgpsu4qz	498	128	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	498	129	of	of	IN
cord-268139-tgpsu4qz	498	130	the	the	DT
cord-268139-tgpsu4qz	498	131	murine	murine	JJ
cord-268139-tgpsu4qz	498	132	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	498	133	MHV	MHV	NNP
cord-268139-tgpsu4qz	498	134	-	-	HYPH
cord-268139-tgpsu4qz	498	135	A59	A59	NNP
cord-268139-tgpsu4qz	499	1	The	the	DT
cord-268139-tgpsu4qz	499	2	putative	putative	JJ
cord-268139-tgpsu4qz	499	3	helicase	helicase	NN
cord-268139-tgpsu4qz	499	4	of	of	IN
cord-268139-tgpsu4qz	499	5	the	the	DT
cord-268139-tgpsu4qz	499	6	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	499	7	mouse	mouse	NN
cord-268139-tgpsu4qz	499	8	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	499	9	virus	virus	NN
cord-268139-tgpsu4qz	499	10	is	be	VBZ
cord-268139-tgpsu4qz	499	11	processed	process	VBN
cord-268139-tgpsu4qz	499	12	from	from	IN
cord-268139-tgpsu4qz	499	13	the	the	DT
cord-268139-tgpsu4qz	499	14	replicase	replicase	NNP
cord-268139-tgpsu4qz	499	15	gene	gene	NNP
cord-268139-tgpsu4qz	499	16	polyprotein	polyprotein	NNP
cord-268139-tgpsu4qz	499	17	and	and	CC
cord-268139-tgpsu4qz	499	18	localizes	localize	VBZ
cord-268139-tgpsu4qz	499	19	in	in	IN
cord-268139-tgpsu4qz	499	20	complexes	complex	NNS
cord-268139-tgpsu4qz	499	21	that	that	WDT
cord-268139-tgpsu4qz	499	22	are	be	VBP
cord-268139-tgpsu4qz	499	23	active	active	JJ
cord-268139-tgpsu4qz	499	24	in	in	IN
cord-268139-tgpsu4qz	499	25	viral	viral	JJ
cord-268139-tgpsu4qz	499	26	RNA	RNA	NNP
cord-268139-tgpsu4qz	499	27	synthesis	synthesis	NN
cord-268139-tgpsu4qz	499	28	Cleavage	cleavage	NN
cord-268139-tgpsu4qz	499	29	between	between	IN
cord-268139-tgpsu4qz	499	30	replicase	replicase	NN
cord-268139-tgpsu4qz	499	31	proteins	protein	NNS
cord-268139-tgpsu4qz	499	32	p28	p28	CD
cord-268139-tgpsu4qz	499	33	and	and	CC
cord-268139-tgpsu4qz	499	34	p65	p65	NN
cord-268139-tgpsu4qz	499	35	of	of	IN
cord-268139-tgpsu4qz	499	36	mouse	mouse	NNP
cord-268139-tgpsu4qz	499	37	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	499	38	virus	virus	NN
cord-268139-tgpsu4qz	499	39	is	be	VBZ
cord-268139-tgpsu4qz	499	40	not	not	RB
cord-268139-tgpsu4qz	499	41	required	require	VBN
cord-268139-tgpsu4qz	499	42	for	for	IN
cord-268139-tgpsu4qz	499	43	virus	virus	NN
cord-268139-tgpsu4qz	499	44	replication	replication	NN
cord-268139-tgpsu4qz	499	45	Coronavirus	Coronavirus	NNP
cord-268139-tgpsu4qz	499	46	genome	genome	NN
cord-268139-tgpsu4qz	499	47	:	:	:
cord-268139-tgpsu4qz	499	48	prediction	prediction	NN
cord-268139-tgpsu4qz	499	49	of	of	IN
cord-268139-tgpsu4qz	499	50	putative	putative	JJ
cord-268139-tgpsu4qz	499	51	functional	functional	JJ
cord-268139-tgpsu4qz	499	52	domains	domain	NNS
cord-268139-tgpsu4qz	499	53	in	in	IN
cord-268139-tgpsu4qz	499	54	the	the	DT
cord-268139-tgpsu4qz	499	55	non	non	JJ
cord-268139-tgpsu4qz	499	56	-	-	JJ
cord-268139-tgpsu4qz	499	57	structural	structural	JJ
cord-268139-tgpsu4qz	499	58	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	499	59	by	by	IN
cord-268139-tgpsu4qz	499	60	comparative	comparative	JJ
cord-268139-tgpsu4qz	499	61	amino	amino	NN
cord-268139-tgpsu4qz	499	62	acid	acid	NN
cord-268139-tgpsu4qz	499	63	sequence	sequence	NN
cord-268139-tgpsu4qz	499	64	analysis	analysis	NN
cord-268139-tgpsu4qz	499	65	RNA	RNA	NNP
cord-268139-tgpsu4qz	499	66	replication	replication	NN
cord-268139-tgpsu4qz	499	67	of	of	IN
cord-268139-tgpsu4qz	499	68	mouse	mouse	NN
cord-268139-tgpsu4qz	499	69	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	499	70	virus	virus	NN
cord-268139-tgpsu4qz	499	71	takes	take	VBZ
cord-268139-tgpsu4qz	499	72	place	place	NN
cord-268139-tgpsu4qz	499	73	at	at	IN
cord-268139-tgpsu4qz	499	74	doublemembrane	doublemembrane	NNP
cord-268139-tgpsu4qz	499	75	vesicles	vesicle	VBZ
cord-268139-tgpsu4qz	499	76	Genetic	genetic	JJ
cord-268139-tgpsu4qz	499	77	analysis	analysis	NN
cord-268139-tgpsu4qz	499	78	of	of	IN
cord-268139-tgpsu4qz	499	79	the	the	DT
cord-268139-tgpsu4qz	499	80	pestivirus	pestivirus	NNP
cord-268139-tgpsu4qz	499	81	nonstructural	nonstructural	NNP
cord-268139-tgpsu4qz	499	82	coding	code	VBG
cord-268139-tgpsu4qz	499	83	region	region	NN
cord-268139-tgpsu4qz	499	84	:	:	:
cord-268139-tgpsu4qz	499	85	defects	defect	NNS
cord-268139-tgpsu4qz	499	86	in	in	IN
cord-268139-tgpsu4qz	499	87	the	the	DT
cord-268139-tgpsu4qz	499	88	NS5A	NS5A	NNP
cord-268139-tgpsu4qz	499	89	unit	unit	NN
cord-268139-tgpsu4qz	499	90	can	can	MD
cord-268139-tgpsu4qz	499	91	be	be	VB
cord-268139-tgpsu4qz	499	92	complemented	complement	VBN
cord-268139-tgpsu4qz	499	93	in	in	IN
cord-268139-tgpsu4qz	499	94	trans	trans	NNP
cord-268139-tgpsu4qz	499	95	Paired	Paired	NNP
cord-268139-tgpsu4qz	499	96	charge	charge	NN
cord-268139-tgpsu4qz	499	97	-	-	HYPH
cord-268139-tgpsu4qz	499	98	to	to	IN
cord-268139-tgpsu4qz	499	99	-	-	HYPH
cord-268139-tgpsu4qz	499	100	alanine	alanine	NN
cord-268139-tgpsu4qz	499	101	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	499	102	of	of	IN
cord-268139-tgpsu4qz	499	103	dengue	dengue	NN
cord-268139-tgpsu4qz	499	104	virus	virus	NN
cord-268139-tgpsu4qz	499	105	type	type	NN
cord-268139-tgpsu4qz	499	106	4	4	CD
cord-268139-tgpsu4qz	499	107	NS5	NS5	NNP
cord-268139-tgpsu4qz	499	108	generates	generate	VBZ
cord-268139-tgpsu4qz	499	109	mutants	mutant	NNS
cord-268139-tgpsu4qz	499	110	with	with	IN
cord-268139-tgpsu4qz	499	111	temperature	temperature	NN
cord-268139-tgpsu4qz	499	112	-	-	HYPH
cord-268139-tgpsu4qz	499	113	sensitive	sensitive	JJ
cord-268139-tgpsu4qz	499	114	,	,	,
cord-268139-tgpsu4qz	499	115	host	host	NN
cord-268139-tgpsu4qz	499	116	range	range	NN
cord-268139-tgpsu4qz	499	117	,	,	,
cord-268139-tgpsu4qz	499	118	and	and	CC
cord-268139-tgpsu4qz	499	119	mouse	mouse	NN
cord-268139-tgpsu4qz	499	120	attenuation	attenuation	NN
cord-268139-tgpsu4qz	499	121	phenotypes	phenotype	NNS
cord-268139-tgpsu4qz	499	122	Identification	identification	NN
cord-268139-tgpsu4qz	499	123	of	of	IN
cord-268139-tgpsu4qz	499	124	severe	severe	JJ
cord-268139-tgpsu4qz	499	125	acute	acute	JJ
cord-268139-tgpsu4qz	499	126	respiratory	respiratory	JJ
cord-268139-tgpsu4qz	499	127	syndrome	syndrome	NN
cord-268139-tgpsu4qz	499	128	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	499	129	replicase	replicase	NN
cord-268139-tgpsu4qz	499	130	products	product	NNS
cord-268139-tgpsu4qz	499	131	and	and	CC
cord-268139-tgpsu4qz	499	132	characterization	characterization	NN
cord-268139-tgpsu4qz	499	133	of	of	IN
cord-268139-tgpsu4qz	499	134	papain	papain	NN
cord-268139-tgpsu4qz	499	135	-	-	HYPH
cord-268139-tgpsu4qz	499	136	like	like	JJ
cord-268139-tgpsu4qz	499	137	protease	protease	NN
cord-268139-tgpsu4qz	499	138	activity	activity	NN
cord-268139-tgpsu4qz	499	139	Targeted	target	VBN
cord-268139-tgpsu4qz	499	140	construction	construction	NN
cord-268139-tgpsu4qz	499	141	of	of	IN
cord-268139-tgpsu4qz	499	142	temperaturesensitive	temperaturesensitive	JJ
cord-268139-tgpsu4qz	499	143	mutations	mutation	NNS
cord-268139-tgpsu4qz	499	144	in	in	IN
cord-268139-tgpsu4qz	499	145	vaccinia	vaccinia	NN
cord-268139-tgpsu4qz	499	146	virus	virus	NN
cord-268139-tgpsu4qz	499	147	by	by	IN
cord-268139-tgpsu4qz	499	148	replacing	replace	VBG
cord-268139-tgpsu4qz	499	149	clustered	cluster	VBN
cord-268139-tgpsu4qz	499	150	charged	charge	VBD
cord-268139-tgpsu4qz	499	151	residues	residue	NNS
cord-268139-tgpsu4qz	499	152	with	with	IN
cord-268139-tgpsu4qz	499	153	alanine	alanine	JJ
cord-268139-tgpsu4qz	499	154	Mouse	Mouse	NNP
cord-268139-tgpsu4qz	499	155	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	499	156	virus	virus	NN
cord-268139-tgpsu4qz	499	157	(	(	-LRB-
cord-268139-tgpsu4qz	499	158	MHV-2	mhv-2	NN
cord-268139-tgpsu4qz	499	159	)	)	-RRB-
cord-268139-tgpsu4qz	499	160	.	.	.
cord-268139-tgpsu4qz	500	1	Plaque	plaque	NN
cord-268139-tgpsu4qz	500	2	assay	assay	NN
cord-268139-tgpsu4qz	500	3	and	and	CC
cord-268139-tgpsu4qz	500	4	propagation	propagation	NN
cord-268139-tgpsu4qz	500	5	in	in	IN
cord-268139-tgpsu4qz	500	6	mouse	mouse	NN
cord-268139-tgpsu4qz	500	7	cell	cell	NN
cord-268139-tgpsu4qz	500	8	line	line	NN
cord-268139-tgpsu4qz	500	9	DBT	DBT	NNP
cord-268139-tgpsu4qz	500	10	cells	cell	NNS
cord-268139-tgpsu4qz	500	11	Identification	identification	NN
cord-268139-tgpsu4qz	500	12	of	of	IN
cord-268139-tgpsu4qz	500	13	the	the	DT
cord-268139-tgpsu4qz	500	14	murine	murine	JJ
cord-268139-tgpsu4qz	500	15	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	500	16	p28	p28	CD
cord-268139-tgpsu4qz	500	17	cleavage	cleavage	NN
cord-268139-tgpsu4qz	500	18	site	site	NN
cord-268139-tgpsu4qz	500	19	Major	major	JJ
cord-268139-tgpsu4qz	500	20	genetic	genetic	JJ
cord-268139-tgpsu4qz	500	21	marker	marker	NN
cord-268139-tgpsu4qz	500	22	of	of	IN
cord-268139-tgpsu4qz	500	23	nidoviruses	nidoviruses	NNP
cord-268139-tgpsu4qz	500	24	encodes	encode	VBZ
cord-268139-tgpsu4qz	500	25	a	a	DT
cord-268139-tgpsu4qz	500	26	replicative	replicative	JJ
cord-268139-tgpsu4qz	500	27	endoribonuclease	endoribonuclease	NN
cord-268139-tgpsu4qz	500	28	trans	trans	NNP
cord-268139-tgpsu4qz	500	29	-	-	NNP
cord-268139-tgpsu4qz	500	30	Complementation	Complementation	NNP
cord-268139-tgpsu4qz	500	31	of	of	IN
cord-268139-tgpsu4qz	500	32	flavivirus	flavivirus	NNP
cord-268139-tgpsu4qz	500	33	RNA	RNA	NNP
cord-268139-tgpsu4qz	500	34	polymerase	polymerase	NN
cord-268139-tgpsu4qz	500	35	gene	gene	NN
cord-268139-tgpsu4qz	500	36	NS5	ns5	JJ
cord-268139-tgpsu4qz	500	37	by	by	IN
cord-268139-tgpsu4qz	500	38	using	use	VBG
cord-268139-tgpsu4qz	500	39	Kunjin	Kunjin	NNP
cord-268139-tgpsu4qz	500	40	virus	virus	NN
cord-268139-tgpsu4qz	500	41	replicon	replicon	NN
cord-268139-tgpsu4qz	500	42	-	-	HYPH
cord-268139-tgpsu4qz	500	43	expressing	express	VBG
cord-268139-tgpsu4qz	500	44	BHK	BHK	NNP
cord-268139-tgpsu4qz	500	45	cells	cell	NNS
cord-268139-tgpsu4qz	501	1	Coronavirus	Coronavirus	NNP
cord-268139-tgpsu4qz	501	2	protein	protein	NN
cord-268139-tgpsu4qz	501	3	processing	processing	NN
cord-268139-tgpsu4qz	501	4	and	and	CC
cord-268139-tgpsu4qz	501	5	RNA	RNA	NNP
cord-268139-tgpsu4qz	501	6	synthesis	synthesis	NN
cord-268139-tgpsu4qz	501	7	is	be	VBZ
cord-268139-tgpsu4qz	501	8	inhibited	inhibit	VBN
cord-268139-tgpsu4qz	501	9	by	by	IN
cord-268139-tgpsu4qz	501	10	the	the	DT
cord-268139-tgpsu4qz	501	11	cysteine	cysteine	NN
cord-268139-tgpsu4qz	501	12	proteinase	proteinase	NN
cord-268139-tgpsu4qz	501	13	inhibitor	inhibitor	NN
cord-268139-tgpsu4qz	501	14	E64d	E64d	NNS
cord-268139-tgpsu4qz	501	15	Newly	newly	RB
cord-268139-tgpsu4qz	501	16	discovered	discover	VBD
cord-268139-tgpsu4qz	501	17	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	501	18	as	as	IN
cord-268139-tgpsu4qz	501	19	the	the	DT
cord-268139-tgpsu4qz	501	20	primary	primary	JJ
cord-268139-tgpsu4qz	501	21	cause	cause	NN
cord-268139-tgpsu4qz	501	22	of	of	IN
cord-268139-tgpsu4qz	501	23	severe	severe	JJ
cord-268139-tgpsu4qz	501	24	acute	acute	JJ
cord-268139-tgpsu4qz	501	25	respiratory	respiratory	JJ
cord-268139-tgpsu4qz	501	26	syndrome	syndrome	NN
cord-268139-tgpsu4qz	502	1	The	the	DT
cord-268139-tgpsu4qz	502	2	complete	complete	JJ
cord-268139-tgpsu4qz	502	3	sequence	sequence	NN
cord-268139-tgpsu4qz	502	4	(	(	-LRB-
cord-268139-tgpsu4qz	502	5	22	22	CD
cord-268139-tgpsu4qz	502	6	kilobases	kilobase	NNS
cord-268139-tgpsu4qz	502	7	)	)	-RRB-
cord-268139-tgpsu4qz	502	8	of	of	IN
cord-268139-tgpsu4qz	502	9	murine	murine	JJ
cord-268139-tgpsu4qz	502	10	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	502	11	gene	gene	NN
cord-268139-tgpsu4qz	502	12	1	1	CD
cord-268139-tgpsu4qz	502	13	encoding	encode	VBG
cord-268139-tgpsu4qz	502	14	the	the	DT
cord-268139-tgpsu4qz	502	15	putative	putative	JJ
cord-268139-tgpsu4qz	502	16	proteases	protease	NNS
cord-268139-tgpsu4qz	502	17	and	and	CC
cord-268139-tgpsu4qz	502	18	RNA	RNA	NNP
cord-268139-tgpsu4qz	502	19	polymerase	polymerase	NN
cord-268139-tgpsu4qz	502	20	trans	trans	NNP
cord-268139-tgpsu4qz	502	21	-	-	NNP
cord-268139-tgpsu4qz	502	22	Complementation	Complementation	NNP
cord-268139-tgpsu4qz	502	23	of	of	IN
cord-268139-tgpsu4qz	502	24	yellow	yellow	JJ
cord-268139-tgpsu4qz	502	25	fever	fever	NN
cord-268139-tgpsu4qz	502	26	virus	virus	NN
cord-268139-tgpsu4qz	502	27	NS1	ns1	CD
cord-268139-tgpsu4qz	502	28	reveals	reveal	VBZ
cord-268139-tgpsu4qz	502	29	a	a	DT
cord-268139-tgpsu4qz	502	30	role	role	NN
cord-268139-tgpsu4qz	502	31	in	in	IN
cord-268139-tgpsu4qz	502	32	early	early	JJ
cord-268139-tgpsu4qz	502	33	RNA	RNA	NNP
cord-268139-tgpsu4qz	502	34	replication	replication	NN
cord-268139-tgpsu4qz	502	35	Complementation	Complementation	NNP
cord-268139-tgpsu4qz	502	36	analysis	analysis	NN
cord-268139-tgpsu4qz	502	37	of	of	IN
cord-268139-tgpsu4qz	502	38	the	the	DT
cord-268139-tgpsu4qz	502	39	flavivirus	flavivirus	NNP
cord-268139-tgpsu4qz	502	40	Kunjin	Kunjin	NNP
cord-268139-tgpsu4qz	502	41	NS3	NS3	NNP
cord-268139-tgpsu4qz	502	42	and	and	CC
cord-268139-tgpsu4qz	502	43	NS5	ns5	JJ
cord-268139-tgpsu4qz	502	44	proteins	protein	NNS
cord-268139-tgpsu4qz	502	45	defines	define	VBZ
cord-268139-tgpsu4qz	502	46	the	the	DT
cord-268139-tgpsu4qz	502	47	minimal	minimal	JJ
cord-268139-tgpsu4qz	502	48	regions	region	NNS
cord-268139-tgpsu4qz	502	49	essential	essential	JJ
cord-268139-tgpsu4qz	502	50	for	for	IN
cord-268139-tgpsu4qz	502	51	formation	formation	NN
cord-268139-tgpsu4qz	502	52	of	of	IN
cord-268139-tgpsu4qz	502	53	a	a	DT
cord-268139-tgpsu4qz	502	54	replication	replication	NN
cord-268139-tgpsu4qz	502	55	complex	complex	NN
cord-268139-tgpsu4qz	502	56	and	and	CC
cord-268139-tgpsu4qz	502	57	shows	show	VBZ
cord-268139-tgpsu4qz	502	58	a	a	DT
cord-268139-tgpsu4qz	502	59	requirement	requirement	NN
cord-268139-tgpsu4qz	502	60	of	of	IN
cord-268139-tgpsu4qz	502	61	NS3	ns3	RB
cord-268139-tgpsu4qz	502	62	in	in	IN
cord-268139-tgpsu4qz	502	63	cis	cis	NNP
cord-268139-tgpsu4qz	502	64	for	for	IN
cord-268139-tgpsu4qz	502	65	virus	virus	NNP
cord-268139-tgpsu4qz	502	66	assembly	assembly	NNP
cord-268139-tgpsu4qz	502	67	Identification	Identification	NNP
cord-268139-tgpsu4qz	502	68	and	and	CC
cord-268139-tgpsu4qz	502	69	characterization	characterization	NN
cord-268139-tgpsu4qz	502	70	of	of	IN
cord-268139-tgpsu4qz	502	71	a	a	DT
cord-268139-tgpsu4qz	502	72	serine	serine	NN
cord-268139-tgpsu4qz	502	73	-	-	HYPH
cord-268139-tgpsu4qz	502	74	like	like	JJ
cord-268139-tgpsu4qz	502	75	proteinase	proteinase	NN
cord-268139-tgpsu4qz	502	76	of	of	IN
cord-268139-tgpsu4qz	502	77	the	the	DT
cord-268139-tgpsu4qz	502	78	murine	murine	JJ
cord-268139-tgpsu4qz	502	79	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	502	80	MHV	MHV	NNP
cord-268139-tgpsu4qz	502	81	-	-	HYPH
cord-268139-tgpsu4qz	502	82	A59	A59	NNP
cord-268139-tgpsu4qz	502	83	Intracellular	Intracellular	NNP
cord-268139-tgpsu4qz	502	84	and	and	CC
cord-268139-tgpsu4qz	502	85	in	in	IN
cord-268139-tgpsu4qz	502	86	vitro	vitro	FW
cord-268139-tgpsu4qz	502	87	-	-	HYPH
cord-268139-tgpsu4qz	502	88	translated	translate	VBN
cord-268139-tgpsu4qz	502	89	27-kDa	27-kda	CD
cord-268139-tgpsu4qz	502	90	proteins	protein	NNS
cord-268139-tgpsu4qz	502	91	contain	contain	VBP
cord-268139-tgpsu4qz	502	92	the	the	DT
cord-268139-tgpsu4qz	502	93	3C	3c	NN
cord-268139-tgpsu4qz	502	94	-	-	HYPH
cord-268139-tgpsu4qz	502	95	like	like	JJ
cord-268139-tgpsu4qz	502	96	proteinase	proteinase	NN
cord-268139-tgpsu4qz	502	97	activity	activity	NN
cord-268139-tgpsu4qz	502	98	of	of	IN
cord-268139-tgpsu4qz	502	99	the	the	DT
cord-268139-tgpsu4qz	502	100	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	502	101	MHV	MHV	NNP
cord-268139-tgpsu4qz	502	102	-	-	HYPH
cord-268139-tgpsu4qz	502	103	A59	A59	NNP
cord-268139-tgpsu4qz	502	104	Molecular	Molecular	NNP
cord-268139-tgpsu4qz	502	105	cloning	cloning	NN
cord-268139-tgpsu4qz	502	106	of	of	IN
cord-268139-tgpsu4qz	502	107	the	the	DT
cord-268139-tgpsu4qz	502	108	gene	gene	NN
cord-268139-tgpsu4qz	502	109	encoding	encode	VBG
cord-268139-tgpsu4qz	502	110	the	the	DT
cord-268139-tgpsu4qz	502	111	putative	putative	JJ
cord-268139-tgpsu4qz	502	112	polymerase	polymerase	NN
cord-268139-tgpsu4qz	502	113	of	of	IN
cord-268139-tgpsu4qz	502	114	mouse	mouse	NN
cord-268139-tgpsu4qz	502	115	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	502	116	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	502	117	,	,	,
cord-268139-tgpsu4qz	502	118	strain	strain	NN
cord-268139-tgpsu4qz	503	1	A59	A59	NNP
cord-268139-tgpsu4qz	503	2	Coronavirus	Coronavirus	NNP
cord-268139-tgpsu4qz	503	3	replication	replication	NN
cord-268139-tgpsu4qz	503	4	complex	complex	JJ
cord-268139-tgpsu4qz	503	5	formation	formation	NN
cord-268139-tgpsu4qz	503	6	utilizes	utilize	VBZ
cord-268139-tgpsu4qz	503	7	components	component	NNS
cord-268139-tgpsu4qz	503	8	of	of	IN
cord-268139-tgpsu4qz	503	9	cellular	cellular	JJ
cord-268139-tgpsu4qz	503	10	autophagy	autophagy	NN
cord-268139-tgpsu4qz	503	11	Identification	identification	NN
cord-268139-tgpsu4qz	503	12	and	and	CC
cord-268139-tgpsu4qz	503	13	characterization	characterization	NN
cord-268139-tgpsu4qz	503	14	of	of	IN
cord-268139-tgpsu4qz	503	15	severe	severe	JJ
cord-268139-tgpsu4qz	503	16	acute	acute	JJ
cord-268139-tgpsu4qz	503	17	respiratory	respiratory	JJ
cord-268139-tgpsu4qz	503	18	syndrome	syndrome	NN
cord-268139-tgpsu4qz	503	19	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	503	20	replicase	replicase	NN
cord-268139-tgpsu4qz	503	21	proteins	protein	NNS
cord-268139-tgpsu4qz	503	22	Processing	process	VBG
cord-268139-tgpsu4qz	503	23	of	of	IN
cord-268139-tgpsu4qz	503	24	the	the	DT
cord-268139-tgpsu4qz	503	25	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	503	26	MHV	MHV	NNP
cord-268139-tgpsu4qz	503	27	-	-	HYPH
cord-268139-tgpsu4qz	503	28	JHM	JHM	NNP
cord-268139-tgpsu4qz	503	29	polymerase	polymerase	NN
cord-268139-tgpsu4qz	503	30	polyprotein	polyprotein	NN
cord-268139-tgpsu4qz	503	31	:	:	:
cord-268139-tgpsu4qz	503	32	identification	identification	NN
cord-268139-tgpsu4qz	503	33	of	of	IN
cord-268139-tgpsu4qz	503	34	precursors	precursor	NNS
cord-268139-tgpsu4qz	503	35	and	and	CC
cord-268139-tgpsu4qz	503	36	proteolytic	proteolytic	JJ
cord-268139-tgpsu4qz	503	37	products	product	NNS
cord-268139-tgpsu4qz	503	38	spanning	span	VBG
cord-268139-tgpsu4qz	503	39	400	400	CD
cord-268139-tgpsu4qz	503	40	kilodaltons	kilodalton	NNS
cord-268139-tgpsu4qz	503	41	of	of	IN
cord-268139-tgpsu4qz	503	42	ORF1a	ORF1a	NNP
cord-268139-tgpsu4qz	503	43	Guanosine	Guanosine	NNP
cord-268139-tgpsu4qz	503	44	triphosphatase	triphosphatase	NN
cord-268139-tgpsu4qz	503	45	activity	activity	NN
cord-268139-tgpsu4qz	503	46	of	of	IN
cord-268139-tgpsu4qz	503	47	the	the	DT
cord-268139-tgpsu4qz	503	48	human	human	JJ
cord-268139-tgpsu4qz	503	49	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	503	50	helicase	helicase	NNP
cord-268139-tgpsu4qz	504	1	The	the	DT
cord-268139-tgpsu4qz	504	2	human	human	JJ
cord-268139-tgpsu4qz	504	3	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	504	4	229E	229e	CD
cord-268139-tgpsu4qz	504	5	superfamily	superfamily	NN
cord-268139-tgpsu4qz	504	6	1	1	CD
cord-268139-tgpsu4qz	504	7	helicase	helicase	NNP
cord-268139-tgpsu4qz	504	8	has	have	VBZ
cord-268139-tgpsu4qz	504	9	RNA	RNA	NNP
cord-268139-tgpsu4qz	504	10	and	and	CC
cord-268139-tgpsu4qz	504	11	DNA	DNA	NNP
cord-268139-tgpsu4qz	504	12	duplexunwinding	duplexunwinding	NN
cord-268139-tgpsu4qz	504	13	activities	activity	NNS
cord-268139-tgpsu4qz	504	14	with	with	IN
cord-268139-tgpsu4qz	504	15	5V	5v	NN
cord-268139-tgpsu4qz	504	16	-	-	HYPH
cord-268139-tgpsu4qz	504	17	to-3V	to-3v	NN
cord-268139-tgpsu4qz	504	18	polarity	polarity	NN
cord-268139-tgpsu4qz	504	19	Colocalization	Colocalization	NNP
cord-268139-tgpsu4qz	504	20	and	and	CC
cord-268139-tgpsu4qz	504	21	membrane	membrane	NNP
cord-268139-tgpsu4qz	504	22	association	association	NNP
cord-268139-tgpsu4qz	504	23	of	of	IN
cord-268139-tgpsu4qz	504	24	murine	murine	NNP
cord-268139-tgpsu4qz	504	25	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	504	26	virus	virus	NN
cord-268139-tgpsu4qz	504	27	gene	gene	NN
cord-268139-tgpsu4qz	504	28	1	1	CD
cord-268139-tgpsu4qz	504	29	products	product	NNS
cord-268139-tgpsu4qz	504	30	and	and	CC
cord-268139-tgpsu4qz	504	31	de	de	NNP
cord-268139-tgpsu4qz	504	32	novo	novo	VB
cord-268139-tgpsu4qz	504	33	-	-	HYPH
cord-268139-tgpsu4qz	504	34	synthesized	synthesize	VBN
cord-268139-tgpsu4qz	504	35	viral	viral	JJ
cord-268139-tgpsu4qz	504	36	RNA	RNA	NNP
cord-268139-tgpsu4qz	504	37	in	in	IN
cord-268139-tgpsu4qz	504	38	infected	infected	JJ
cord-268139-tgpsu4qz	504	39	cells	cell	NNS
cord-268139-tgpsu4qz	505	1	Mouse	Mouse	NNP
cord-268139-tgpsu4qz	505	2	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	505	3	virus	virus	NN
cord-268139-tgpsu4qz	505	4	replicase	replicase	NN
cord-268139-tgpsu4qz	505	5	proteins	protein	NNS
cord-268139-tgpsu4qz	505	6	associate	associate	VBP
cord-268139-tgpsu4qz	505	7	with	with	IN
cord-268139-tgpsu4qz	505	8	two	two	CD
cord-268139-tgpsu4qz	505	9	distinct	distinct	JJ
cord-268139-tgpsu4qz	505	10	populations	population	NNS
cord-268139-tgpsu4qz	505	11	of	of	IN
cord-268139-tgpsu4qz	505	12	intracellular	intracellular	JJ
cord-268139-tgpsu4qz	505	13	membranes	membrane	NNS
cord-268139-tgpsu4qz	505	14	Unique	unique	JJ
cord-268139-tgpsu4qz	505	15	and	and	CC
cord-268139-tgpsu4qz	505	16	conserved	conserve	VBN
cord-268139-tgpsu4qz	505	17	features	feature	NNS
cord-268139-tgpsu4qz	505	18	of	of	IN
cord-268139-tgpsu4qz	505	19	genome	genome	NN
cord-268139-tgpsu4qz	505	20	and	and	CC
cord-268139-tgpsu4qz	505	21	proteome	proteome	NN
cord-268139-tgpsu4qz	505	22	of	of	IN
cord-268139-tgpsu4qz	505	23	SARScoronavirus	SARScoronavirus	NNP
cord-268139-tgpsu4qz	505	24	,	,	,
cord-268139-tgpsu4qz	505	25	an	an	DT
cord-268139-tgpsu4qz	505	26	early	early	JJ
cord-268139-tgpsu4qz	505	27	split	split	NN
cord-268139-tgpsu4qz	505	28	-	-	HYPH
cord-268139-tgpsu4qz	505	29	off	off	NN
cord-268139-tgpsu4qz	505	30	from	from	IN
cord-268139-tgpsu4qz	505	31	the	the	DT
cord-268139-tgpsu4qz	505	32	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	505	33	group	group	NN
cord-268139-tgpsu4qz	505	34	2	2	CD
cord-268139-tgpsu4qz	505	35	lineage	lineage	NN
cord-268139-tgpsu4qz	505	36	Single	single	JJ
cord-268139-tgpsu4qz	505	37	-	-	HYPH
cord-268139-tgpsu4qz	505	38	amino	amino	NN
cord-268139-tgpsu4qz	505	39	-	-	HYPH
cord-268139-tgpsu4qz	505	40	acid	acid	NN
cord-268139-tgpsu4qz	505	41	substitutions	substitution	NNS
cord-268139-tgpsu4qz	505	42	in	in	IN
cord-268139-tgpsu4qz	505	43	open	open	JJ
cord-268139-tgpsu4qz	505	44	reading	reading	NN
cord-268139-tgpsu4qz	505	45	frame	frame	NN
cord-268139-tgpsu4qz	506	1	(	(	-LRB-
cord-268139-tgpsu4qz	506	2	ORF	ORF	NNP
cord-268139-tgpsu4qz	506	3	)	)	-RRB-
cord-268139-tgpsu4qz	507	1	1b	1b	LS
cord-268139-tgpsu4qz	507	2	-	-	HYPH
cord-268139-tgpsu4qz	507	3	nsp14	nsp14	NNP
cord-268139-tgpsu4qz	507	4	and	and	CC
cord-268139-tgpsu4qz	507	5	ORF	ORF	NNP
cord-268139-tgpsu4qz	507	6	2a	2a	CD
cord-268139-tgpsu4qz	507	7	proteins	protein	NNS
cord-268139-tgpsu4qz	507	8	of	of	IN
cord-268139-tgpsu4qz	507	9	the	the	DT
cord-268139-tgpsu4qz	507	10	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	507	11	mouse	mouse	NN
cord-268139-tgpsu4qz	507	12	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	507	13	virus	virus	NN
cord-268139-tgpsu4qz	507	14	are	be	VBP
cord-268139-tgpsu4qz	507	15	attenuating	attenuate	VBG
cord-268139-tgpsu4qz	507	16	in	in	IN
cord-268139-tgpsu4qz	508	1	mice	mouse	NNS
cord-268139-tgpsu4qz	508	2	Clustered	Clustered	NNPS
cord-268139-tgpsu4qz	508	3	charge	charge	NN
cord-268139-tgpsu4qz	508	4	-	-	HYPH
cord-268139-tgpsu4qz	508	5	toalanine	toalanine	NN
cord-268139-tgpsu4qz	508	6	mutagenesis	mutagenesis	NN
cord-268139-tgpsu4qz	508	7	of	of	IN
cord-268139-tgpsu4qz	508	8	human	human	JJ
cord-268139-tgpsu4qz	508	9	respiratory	respiratory	JJ
cord-268139-tgpsu4qz	508	10	syncytial	syncytial	JJ
cord-268139-tgpsu4qz	508	11	virus	virus	NN
cord-268139-tgpsu4qz	508	12	L	l	NN
cord-268139-tgpsu4qz	508	13	polymerase	polymerase	NN
cord-268139-tgpsu4qz	508	14	generates	generate	VBZ
cord-268139-tgpsu4qz	508	15	temperature	temperature	NN
cord-268139-tgpsu4qz	508	16	-	-	HYPH
cord-268139-tgpsu4qz	508	17	sensitive	sensitive	JJ
cord-268139-tgpsu4qz	508	18	viruses	virus	NNS
cord-268139-tgpsu4qz	508	19	Mechanisms	mechanism	NNS
cord-268139-tgpsu4qz	508	20	and	and	CC
cord-268139-tgpsu4qz	508	21	enzymes	enzyme	NNS
cord-268139-tgpsu4qz	508	22	involved	involve	VBN
cord-268139-tgpsu4qz	508	23	in	in	IN
cord-268139-tgpsu4qz	508	24	SARS	SARS	NNP
cord-268139-tgpsu4qz	508	25	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	508	26	genome	genome	NN
cord-268139-tgpsu4qz	508	27	expression	expression	NN
cord-268139-tgpsu4qz	508	28	Localization	Localization	NNP
cord-268139-tgpsu4qz	508	29	of	of	IN
cord-268139-tgpsu4qz	508	30	mouse	mouse	NN
cord-268139-tgpsu4qz	508	31	hepatitis	hepatitis	NN
cord-268139-tgpsu4qz	508	32	virus	virus	NN
cord-268139-tgpsu4qz	508	33	nonstructural	nonstructural	JJ
cord-268139-tgpsu4qz	508	34	proteins	protein	NNS
cord-268139-tgpsu4qz	508	35	and	and	CC
cord-268139-tgpsu4qz	508	36	RNA	RNA	NNP
cord-268139-tgpsu4qz	508	37	synthesis	synthesis	NN
cord-268139-tgpsu4qz	508	38	indicates	indicate	VBZ
cord-268139-tgpsu4qz	508	39	a	a	DT
cord-268139-tgpsu4qz	508	40	role	role	NN
cord-268139-tgpsu4qz	508	41	for	for	IN
cord-268139-tgpsu4qz	508	42	late	late	JJ
cord-268139-tgpsu4qz	508	43	endosomes	endosome	NNS
cord-268139-tgpsu4qz	508	44	in	in	IN
cord-268139-tgpsu4qz	508	45	viral	viral	JJ
cord-268139-tgpsu4qz	508	46	replication	replication	NN
cord-268139-tgpsu4qz	508	47	Systematic	systematic	JJ
cord-268139-tgpsu4qz	508	48	assembly	assembly	NN
cord-268139-tgpsu4qz	508	49	of	of	IN
cord-268139-tgpsu4qz	508	50	a	a	DT
cord-268139-tgpsu4qz	508	51	full	full	JJ
cord-268139-tgpsu4qz	508	52	-	-	HYPH
cord-268139-tgpsu4qz	508	53	length	length	NN
cord-268139-tgpsu4qz	508	54	infectious	infectious	JJ
cord-268139-tgpsu4qz	508	55	cDNA	cdna	NN
cord-268139-tgpsu4qz	508	56	of	of	IN
cord-268139-tgpsu4qz	508	57	mouse	mouse	NNP
cord-268139-tgpsu4qz	508	58	hepatitis	hepatitis	NNP
cord-268139-tgpsu4qz	508	59	virus	virus	NN
cord-268139-tgpsu4qz	508	60	strain	strain	VBP
cord-268139-tgpsu4qz	508	61	A59	A59	NNP
cord-268139-tgpsu4qz	509	1	The	the	DT
cord-268139-tgpsu4qz	509	2	coronavirus	coronavirus	NN
cord-268139-tgpsu4qz	509	3	replicase	replicase	NN
cord-268139-tgpsu4qz	509	4	We	-PRON-	PRP
cord-268139-tgpsu4qz	509	5	express	express	VBP
cord-268139-tgpsu4qz	509	6	our	-PRON-	PRP$
cord-268139-tgpsu4qz	509	7	appreciation	appreciation	NN
cord-268139-tgpsu4qz	509	8	to	to	IN
cord-268139-tgpsu4qz	509	9	Xiao	Xiao	NNP
cord-268139-tgpsu4qz	509	10	Tao	Tao	NNP
cord-268139-tgpsu4qz	509	11	Lu	Lu	NNP
cord-268139-tgpsu4qz	509	12	 	 	_SP