id sid tid token lemma pos cord-268467-btfz6ye8 1 1 key key JJ cord-268467-btfz6ye8 1 2 : : : cord-268467-btfz6ye8 1 3 cord-268467-btfz6ye8 cord-268467-btfz6ye8 NNS cord-268467-btfz6ye8 1 4 authors author NNS cord-268467-btfz6ye8 1 5 : : : cord-268467-btfz6ye8 1 6 Schreiber Schreiber NNP cord-268467-btfz6ye8 1 7 , , , cord-268467-btfz6ye8 1 8 Steven Steven NNP cord-268467-btfz6ye8 1 9 S. S. NNP cord-268467-btfz6ye8 1 10 ; ; : cord-268467-btfz6ye8 1 11 Kamahora Kamahora NNP cord-268467-btfz6ye8 1 12 , , , cord-268467-btfz6ye8 1 13 Toshio Toshio NNP cord-268467-btfz6ye8 1 14 ; ; : cord-268467-btfz6ye8 1 15 Lai Lai NNP cord-268467-btfz6ye8 1 16 , , , cord-268467-btfz6ye8 1 17 Michael Michael NNP cord-268467-btfz6ye8 1 18 M.C. M.C. NNP cord-268467-btfz6ye8 1 19 title title NN cord-268467-btfz6ye8 1 20 : : : cord-268467-btfz6ye8 2 1 Sequence sequence NN cord-268467-btfz6ye8 2 2 analysis analysis NN cord-268467-btfz6ye8 2 3 of of IN cord-268467-btfz6ye8 2 4 the the DT cord-268467-btfz6ye8 2 5 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 2 6 protein protein NNP cord-268467-btfz6ye8 2 7 gene gene NN cord-268467-btfz6ye8 2 8 of of IN cord-268467-btfz6ye8 2 9 human human JJ cord-268467-btfz6ye8 2 10 coronavirus coronavirus NN cord-268467-btfz6ye8 2 11 229E 229e CD cord-268467-btfz6ye8 2 12 date date NN cord-268467-btfz6ye8 2 13 : : : cord-268467-btfz6ye8 2 14 1989 1989 CD cord-268467-btfz6ye8 2 15 - - HYPH cord-268467-btfz6ye8 2 16 03 03 CD cord-268467-btfz6ye8 2 17 - - HYPH cord-268467-btfz6ye8 2 18 31 31 CD cord-268467-btfz6ye8 2 19 journal journal NN cord-268467-btfz6ye8 2 20 : : : cord-268467-btfz6ye8 3 1 Virology virology NN cord-268467-btfz6ye8 3 2 DOI DOI NNP cord-268467-btfz6ye8 3 3 : : : cord-268467-btfz6ye8 3 4 10.1016/0042 10.1016/0042 CD cord-268467-btfz6ye8 3 5 - - HYPH cord-268467-btfz6ye8 3 6 6822(89)90050 6822(89)90050 NNP cord-268467-btfz6ye8 3 7 - - HYPH cord-268467-btfz6ye8 3 8 0 0 CD cord-268467-btfz6ye8 4 1 sha sha NNP cord-268467-btfz6ye8 4 2 : : : cord-268467-btfz6ye8 4 3 3c1a8c61f6c227dc2eadb791ab941ed5db582092 3c1a8c61f6c227dc2eadb791ab941ed5db582092 CD cord-268467-btfz6ye8 4 4 doc_id doc_id CD cord-268467-btfz6ye8 4 5 : : : cord-268467-btfz6ye8 4 6 268467 268467 CD cord-268467-btfz6ye8 4 7 cord_uid cord_uid FW cord-268467-btfz6ye8 4 8 : : : cord-268467-btfz6ye8 5 1 btfz6ye8 btfz6ye8 CD cord-268467-btfz6ye8 5 2 Abstract Abstract NNP cord-268467-btfz6ye8 5 3 Human Human NNP cord-268467-btfz6ye8 5 4 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 5 5 are be VBP cord-268467-btfz6ye8 5 6 important important JJ cord-268467-btfz6ye8 5 7 human human JJ cord-268467-btfz6ye8 5 8 pathogens pathogen NNS cord-268467-btfz6ye8 5 9 and and CC cord-268467-btfz6ye8 5 10 have have VBP cord-268467-btfz6ye8 5 11 also also RB cord-268467-btfz6ye8 5 12 been be VBN cord-268467-btfz6ye8 5 13 implicated implicate VBN cord-268467-btfz6ye8 5 14 in in IN cord-268467-btfz6ye8 5 15 multiple multiple JJ cord-268467-btfz6ye8 5 16 sclerosis sclerosis NN cord-268467-btfz6ye8 5 17 . . . cord-268467-btfz6ye8 6 1 To to TO cord-268467-btfz6ye8 6 2 further further RB cord-268467-btfz6ye8 6 3 understand understand VB cord-268467-btfz6ye8 6 4 the the DT cord-268467-btfz6ye8 6 5 molecular molecular JJ cord-268467-btfz6ye8 6 6 biology biology NN cord-268467-btfz6ye8 6 7 of of IN cord-268467-btfz6ye8 6 8 human human JJ cord-268467-btfz6ye8 6 9 coronavirus coronavirus NN cord-268467-btfz6ye8 6 10 229E 229e CD cord-268467-btfz6ye8 7 1 ( ( -LRB- cord-268467-btfz6ye8 7 2 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 7 3 ) ) -RRB- cord-268467-btfz6ye8 7 4 , , , cord-268467-btfz6ye8 7 5 molecular molecular JJ cord-268467-btfz6ye8 7 6 cloning cloning NN cord-268467-btfz6ye8 7 7 and and CC cord-268467-btfz6ye8 7 8 sequence sequence NN cord-268467-btfz6ye8 7 9 analysis analysis NN cord-268467-btfz6ye8 7 10 of of IN cord-268467-btfz6ye8 7 11 the the DT cord-268467-btfz6ye8 7 12 viral viral JJ cord-268467-btfz6ye8 7 13 RNA RNA NNP cord-268467-btfz6ye8 7 14 have have VBP cord-268467-btfz6ye8 7 15 been be VBN cord-268467-btfz6ye8 7 16 initiated initiate VBN cord-268467-btfz6ye8 7 17 . . . cord-268467-btfz6ye8 8 1 Following follow VBG cord-268467-btfz6ye8 8 2 established establish VBN cord-268467-btfz6ye8 8 3 protocols protocol NNS cord-268467-btfz6ye8 8 4 , , , cord-268467-btfz6ye8 8 5 the the DT cord-268467-btfz6ye8 8 6 3′-terminal 3′-terminal CD cord-268467-btfz6ye8 8 7 1732 1732 CD cord-268467-btfz6ye8 8 8 nucleotides nucleotide NNS cord-268467-btfz6ye8 8 9 of of IN cord-268467-btfz6ye8 8 10 the the DT cord-268467-btfz6ye8 8 11 genome genome NN cord-268467-btfz6ye8 8 12 were be VBD cord-268467-btfz6ye8 8 13 sequenced sequence VBN cord-268467-btfz6ye8 8 14 . . . cord-268467-btfz6ye8 9 1 A a DT cord-268467-btfz6ye8 9 2 large large JJ cord-268467-btfz6ye8 9 3 open open JJ cord-268467-btfz6ye8 9 4 reading reading NN cord-268467-btfz6ye8 9 5 frame frame NN cord-268467-btfz6ye8 9 6 encodes encode VBZ cord-268467-btfz6ye8 9 7 a a DT cord-268467-btfz6ye8 9 8 389 389 CD cord-268467-btfz6ye8 9 9 amino amino NN cord-268467-btfz6ye8 9 10 acid acid NN cord-268467-btfz6ye8 9 11 protein protein NN cord-268467-btfz6ye8 9 12 of of IN cord-268467-btfz6ye8 9 13 43,366 43,366 CD cord-268467-btfz6ye8 9 14 Da Da NNP cord-268467-btfz6ye8 9 15 , , , cord-268467-btfz6ye8 9 16 which which WDT cord-268467-btfz6ye8 9 17 is be VBZ cord-268467-btfz6ye8 9 18 presumably presumably RB cord-268467-btfz6ye8 9 19 the the DT cord-268467-btfz6ye8 9 20 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 9 21 protein protein NN cord-268467-btfz6ye8 9 22 . . . cord-268467-btfz6ye8 10 1 The the DT cord-268467-btfz6ye8 10 2 predicted predict VBN cord-268467-btfz6ye8 10 3 protein protein NN cord-268467-btfz6ye8 10 4 is be VBZ cord-268467-btfz6ye8 10 5 similar similar JJ cord-268467-btfz6ye8 10 6 in in IN cord-268467-btfz6ye8 10 7 size size NN cord-268467-btfz6ye8 10 8 , , , cord-268467-btfz6ye8 10 9 chemical chemical NN cord-268467-btfz6ye8 10 10 properties property NNS cord-268467-btfz6ye8 10 11 , , , cord-268467-btfz6ye8 10 12 and and CC cord-268467-btfz6ye8 10 13 amino amino NN cord-268467-btfz6ye8 10 14 acid acid NN cord-268467-btfz6ye8 10 15 sequence sequence NN cord-268467-btfz6ye8 10 16 to to IN cord-268467-btfz6ye8 10 17 the the DT cord-268467-btfz6ye8 10 18 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 10 19 proteins protein NNS cord-268467-btfz6ye8 10 20 of of IN cord-268467-btfz6ye8 10 21 other other JJ cord-268467-btfz6ye8 10 22 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 10 23 . . . cord-268467-btfz6ye8 11 1 This this DT cord-268467-btfz6ye8 11 2 is be VBZ cord-268467-btfz6ye8 11 3 especially especially RB cord-268467-btfz6ye8 11 4 evident evident JJ cord-268467-btfz6ye8 11 5 when when WRB cord-268467-btfz6ye8 11 6 the the DT cord-268467-btfz6ye8 11 7 sequence sequence NN cord-268467-btfz6ye8 11 8 is be VBZ cord-268467-btfz6ye8 11 9 compared compare VBN cord-268467-btfz6ye8 11 10 with with IN cord-268467-btfz6ye8 11 11 that that DT cord-268467-btfz6ye8 11 12 of of IN cord-268467-btfz6ye8 11 13 the the DT cord-268467-btfz6ye8 11 14 antigenically antigenically RB cord-268467-btfz6ye8 11 15 related relate VBN cord-268467-btfz6ye8 11 16 porcine porcine JJ cord-268467-btfz6ye8 11 17 transmissible transmissible JJ cord-268467-btfz6ye8 11 18 gastroenteritis gastroenteritis NN cord-268467-btfz6ye8 11 19 virus virus NN cord-268467-btfz6ye8 11 20 ( ( -LRB- cord-268467-btfz6ye8 11 21 TGEV TGEV NNP cord-268467-btfz6ye8 11 22 ) ) -RRB- cord-268467-btfz6ye8 11 23 , , , cord-268467-btfz6ye8 11 24 with with IN cord-268467-btfz6ye8 11 25 which which WDT cord-268467-btfz6ye8 11 26 a a DT cord-268467-btfz6ye8 11 27 region region NN cord-268467-btfz6ye8 11 28 of of IN cord-268467-btfz6ye8 11 29 46 46 CD cord-268467-btfz6ye8 11 30 % % NN cord-268467-btfz6ye8 11 31 amino amino NN cord-268467-btfz6ye8 11 32 acid acid NN cord-268467-btfz6ye8 11 33 sequence sequence NN cord-268467-btfz6ye8 11 34 homology homology NN cord-268467-btfz6ye8 11 35 was be VBD cord-268467-btfz6ye8 11 36 found find VBN cord-268467-btfz6ye8 11 37 . . . cord-268467-btfz6ye8 12 1 Hydropathy Hydropathy NNP cord-268467-btfz6ye8 12 2 profiles profile NNS cord-268467-btfz6ye8 12 3 revealed reveal VBD cord-268467-btfz6ye8 12 4 the the DT cord-268467-btfz6ye8 12 5 existence existence NN cord-268467-btfz6ye8 12 6 of of IN cord-268467-btfz6ye8 12 7 several several JJ cord-268467-btfz6ye8 12 8 conserved conserve VBN cord-268467-btfz6ye8 12 9 domains domain NNS cord-268467-btfz6ye8 12 10 which which WDT cord-268467-btfz6ye8 12 11 could could MD cord-268467-btfz6ye8 12 12 have have VB cord-268467-btfz6ye8 12 13 functional functional JJ cord-268467-btfz6ye8 12 14 significance significance NN cord-268467-btfz6ye8 12 15 . . . cord-268467-btfz6ye8 13 1 An an DT cord-268467-btfz6ye8 13 2 intergenic intergenic JJ cord-268467-btfz6ye8 13 3 consensus consensus NN cord-268467-btfz6ye8 13 4 sequence sequence NN cord-268467-btfz6ye8 13 5 precedes precede VBZ cord-268467-btfz6ye8 13 6 the the DT cord-268467-btfz6ye8 13 7 5′-end 5′-end CD cord-268467-btfz6ye8 13 8 of of IN cord-268467-btfz6ye8 13 9 the the DT cord-268467-btfz6ye8 13 10 proposed propose VBN cord-268467-btfz6ye8 13 11 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 13 12 protein protein NN cord-268467-btfz6ye8 13 13 gene gene NN cord-268467-btfz6ye8 13 14 . . . cord-268467-btfz6ye8 14 1 The the DT cord-268467-btfz6ye8 14 2 consensus consensus NN cord-268467-btfz6ye8 14 3 sequence sequence NN cord-268467-btfz6ye8 14 4 is be VBZ cord-268467-btfz6ye8 14 5 present present JJ cord-268467-btfz6ye8 14 6 in in IN cord-268467-btfz6ye8 14 7 other other JJ cord-268467-btfz6ye8 14 8 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 14 9 and and CC cord-268467-btfz6ye8 14 10 has have VBZ cord-268467-btfz6ye8 14 11 been be VBN cord-268467-btfz6ye8 14 12 proposed propose VBN cord-268467-btfz6ye8 14 13 as as IN cord-268467-btfz6ye8 14 14 the the DT cord-268467-btfz6ye8 14 15 site site NN cord-268467-btfz6ye8 14 16 of of IN cord-268467-btfz6ye8 14 17 binding binding NN cord-268467-btfz6ye8 14 18 of of IN cord-268467-btfz6ye8 14 19 the the DT cord-268467-btfz6ye8 14 20 leader leader NN cord-268467-btfz6ye8 14 21 sequence sequence NN cord-268467-btfz6ye8 14 22 for for IN cord-268467-btfz6ye8 14 23 mRNA mRNA NNP cord-268467-btfz6ye8 14 24 transcriptional transcriptional JJ cord-268467-btfz6ye8 14 25 start start NN cord-268467-btfz6ye8 14 26 . . . cord-268467-btfz6ye8 15 1 This this DT cord-268467-btfz6ye8 15 2 region region NN cord-268467-btfz6ye8 15 3 was be VBD cord-268467-btfz6ye8 15 4 also also RB cord-268467-btfz6ye8 15 5 examined examine VBN cord-268467-btfz6ye8 15 6 by by IN cord-268467-btfz6ye8 15 7 primer primer NN cord-268467-btfz6ye8 15 8 extension extension NN cord-268467-btfz6ye8 15 9 analysis analysis NN cord-268467-btfz6ye8 15 10 of of IN cord-268467-btfz6ye8 15 11 mRNAs mrnas CD cord-268467-btfz6ye8 15 12 , , , cord-268467-btfz6ye8 15 13 which which WDT cord-268467-btfz6ye8 15 14 identified identify VBD cord-268467-btfz6ye8 15 15 a a DT cord-268467-btfz6ye8 15 16 60-nucleotide 60-nucleotide CD cord-268467-btfz6ye8 15 17 leader leader NN cord-268467-btfz6ye8 15 18 sequence sequence NN cord-268467-btfz6ye8 15 19 . . . cord-268467-btfz6ye8 16 1 The the DT cord-268467-btfz6ye8 16 2 3′-noncoding 3′-noncoding CD cord-268467-btfz6ye8 16 3 region region NN cord-268467-btfz6ye8 16 4 of of IN cord-268467-btfz6ye8 16 5 the the DT cord-268467-btfz6ye8 16 6 genome genome NN cord-268467-btfz6ye8 16 7 contains contain VBZ cord-268467-btfz6ye8 16 8 an an DT cord-268467-btfz6ye8 16 9 11-nucleotide 11-nucleotide CD cord-268467-btfz6ye8 16 10 sequence sequence NN cord-268467-btfz6ye8 16 11 , , , cord-268467-btfz6ye8 16 12 which which WDT cord-268467-btfz6ye8 16 13 is be VBZ cord-268467-btfz6ye8 16 14 relatively relatively RB cord-268467-btfz6ye8 16 15 conserved conserved JJ cord-268467-btfz6ye8 16 16 throughout throughout IN cord-268467-btfz6ye8 16 17 the the DT cord-268467-btfz6ye8 16 18 Coronavirus Coronavirus NNP cord-268467-btfz6ye8 16 19 family family NN cord-268467-btfz6ye8 16 20 and and CC cord-268467-btfz6ye8 16 21 lends lend VBZ cord-268467-btfz6ye8 16 22 support support NN cord-268467-btfz6ye8 16 23 to to IN cord-268467-btfz6ye8 16 24 the the DT cord-268467-btfz6ye8 16 25 theory theory NN cord-268467-btfz6ye8 16 26 that that IN cord-268467-btfz6ye8 16 27 this this DT cord-268467-btfz6ye8 16 28 region region NN cord-268467-btfz6ye8 16 29 is be VBZ cord-268467-btfz6ye8 16 30 important important JJ cord-268467-btfz6ye8 16 31 for for IN cord-268467-btfz6ye8 16 32 the the DT cord-268467-btfz6ye8 16 33 replication replication NN cord-268467-btfz6ye8 16 34 of of IN cord-268467-btfz6ye8 16 35 negative negative JJ cord-268467-btfz6ye8 16 36 - - HYPH cord-268467-btfz6ye8 16 37 strand strand NN cord-268467-btfz6ye8 16 38 RNA RNA NNP cord-268467-btfz6ye8 16 39 . . . cord-268467-btfz6ye8 17 1 Human human JJ cord-268467-btfz6ye8 17 2 coronavirus coronavirus NN cord-268467-btfz6ye8 17 3 229E 229e CD cord-268467-btfz6ye8 18 1 ( ( -LRB- cord-268467-btfz6ye8 18 2 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 18 3 ) ) -RRB- cord-268467-btfz6ye8 18 4 belongs belong VBZ cord-268467-btfz6ye8 18 5 to to IN cord-268467-btfz6ye8 18 6 one one CD cord-268467-btfz6ye8 18 7 of of IN cord-268467-btfz6ye8 18 8 two two CD cord-268467-btfz6ye8 18 9 major major JJ cord-268467-btfz6ye8 18 10 antigenic antigenic JJ cord-268467-btfz6ye8 18 11 groups group NNS cord-268467-btfz6ye8 18 12 of of IN cord-268467-btfz6ye8 18 13 human human JJ cord-268467-btfz6ye8 18 14 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 18 15 ( ( -LRB- cord-268467-btfz6ye8 18 16 MacNaughton MacNaughton NNP cord-268467-btfz6ye8 18 17 , , , cord-268467-btfz6ye8 18 18 1981 1981 CD cord-268467-btfz6ye8 18 19 ) ) -RRB- cord-268467-btfz6ye8 18 20 . . . cord-268467-btfz6ye8 19 1 It -PRON- PRP cord-268467-btfz6ye8 19 2 shares share VBZ cord-268467-btfz6ye8 19 3 antigenic antigenic JJ cord-268467-btfz6ye8 19 4 relationships relationship NNS cord-268467-btfz6ye8 19 5 with with IN cord-268467-btfz6ye8 19 6 other other JJ cord-268467-btfz6ye8 19 7 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 19 8 , , , cord-268467-btfz6ye8 19 9 such such JJ cord-268467-btfz6ye8 19 10 as as IN cord-268467-btfz6ye8 19 11 porcine porcine JJ cord-268467-btfz6ye8 19 12 transmissible transmissible JJ cord-268467-btfz6ye8 19 13 gastroenteritis gastroenteritis NN cord-268467-btfz6ye8 19 14 virus virus NN cord-268467-btfz6ye8 19 15 ( ( -LRB- cord-268467-btfz6ye8 19 16 TGEV TGEV NNP cord-268467-btfz6ye8 19 17 ) ) -RRB- cord-268467-btfz6ye8 19 18 , , , cord-268467-btfz6ye8 19 19 feline feline JJ cord-268467-btfz6ye8 19 20 infectious infectious JJ cord-268467-btfz6ye8 19 21 peritonitis peritonitis NN cord-268467-btfz6ye8 19 22 virus virus NN cord-268467-btfz6ye8 19 23 ( ( -LRB- cord-268467-btfz6ye8 19 24 FIPV FIPV NNP cord-268467-btfz6ye8 19 25 ) ) -RRB- cord-268467-btfz6ye8 19 26 , , , cord-268467-btfz6ye8 19 27 and and CC cord-268467-btfz6ye8 19 28 canine canine JJ cord-268467-btfz6ye8 19 29 coronavirus coronavirus NN cord-268467-btfz6ye8 19 30 ( ( -LRB- cord-268467-btfz6ye8 19 31 CCV CCV NNP cord-268467-btfz6ye8 19 32 ) ) -RRB- cord-268467-btfz6ye8 19 33 . . . cord-268467-btfz6ye8 20 1 The the DT cord-268467-btfz6ye8 20 2 other other JJ cord-268467-btfz6ye8 20 3 well well RB cord-268467-btfz6ye8 20 4 - - HYPH cord-268467-btfz6ye8 20 5 characterized characterize VBN cord-268467-btfz6ye8 20 6 human human JJ cord-268467-btfz6ye8 20 7 coronavirus coronavirus NN cord-268467-btfz6ye8 20 8 , , , cord-268467-btfz6ye8 20 9 HCV HCV NNP cord-268467-btfz6ye8 20 10 - - HYPH cord-268467-btfz6ye8 20 11 OC43 OC43 NNP cord-268467-btfz6ye8 20 12 , , , cord-268467-btfz6ye8 20 13 is be VBZ cord-268467-btfz6ye8 20 14 in in IN cord-268467-btfz6ye8 20 15 a a DT cord-268467-btfz6ye8 20 16 separate separate JJ cord-268467-btfz6ye8 20 17 antigenic antigenic JJ cord-268467-btfz6ye8 20 18 group group NN cord-268467-btfz6ye8 20 19 which which WDT cord-268467-btfz6ye8 20 20 includes include VBZ cord-268467-btfz6ye8 20 21 mouse mouse NN cord-268467-btfz6ye8 20 22 hepatitis hepatitis NN cord-268467-btfz6ye8 20 23 virus virus NN cord-268467-btfz6ye8 20 24 ( ( -LRB- cord-268467-btfz6ye8 20 25 MHV MHV NNP cord-268467-btfz6ye8 20 26 ) ) -RRB- cord-268467-btfz6ye8 20 27 and and CC cord-268467-btfz6ye8 20 28 bovine bovine JJ cord-268467-btfz6ye8 20 29 coronavirus coronavirus NN cord-268467-btfz6ye8 20 30 ( ( -LRB- cord-268467-btfz6ye8 20 31 BCV BCV NNP cord-268467-btfz6ye8 20 32 ) ) -RRB- cord-268467-btfz6ye8 20 33 . . . cord-268467-btfz6ye8 21 1 Both both DT cord-268467-btfz6ye8 21 2 human human JJ cord-268467-btfz6ye8 21 3 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 21 4 are be VBP cord-268467-btfz6ye8 21 5 mainly mainly RB cord-268467-btfz6ye8 21 6 respiratory respiratory JJ cord-268467-btfz6ye8 21 7 pathogens pathogen NNS cord-268467-btfz6ye8 21 8 and and CC cord-268467-btfz6ye8 21 9 have have VBP cord-268467-btfz6ye8 21 10 been be VBN cord-268467-btfz6ye8 21 11 estimated estimate VBN cord-268467-btfz6ye8 21 12 to to TO cord-268467-btfz6ye8 21 13 cause cause VB cord-268467-btfz6ye8 21 14 up up IN cord-268467-btfz6ye8 21 15 to to TO cord-268467-btfz6ye8 21 16 25 25 CD cord-268467-btfz6ye8 21 17 % % NN cord-268467-btfz6ye8 21 18 of of IN cord-268467-btfz6ye8 21 19 common common JJ cord-268467-btfz6ye8 21 20 colds cold NNS cord-268467-btfz6ye8 21 21 ( ( -LRB- cord-268467-btfz6ye8 21 22 McIntosh McIntosh NNP cord-268467-btfz6ye8 21 23 et et NNP cord-268467-btfz6ye8 21 24 a/. a/. NNP cord-268467-btfz6ye8 21 25 , , , cord-268467-btfz6ye8 21 26 1974 1974 CD cord-268467-btfz6ye8 21 27 ; ; : cord-268467-btfz6ye8 21 28 Wege Wege NNP cord-268467-btfz6ye8 21 29 et et NNP cord-268467-btfz6ye8 21 30 a/. a/. NNP cord-268467-btfz6ye8 21 31 , , , cord-268467-btfz6ye8 21 32 1982 1982 CD cord-268467-btfz6ye8 21 33 ) ) -RRB- cord-268467-btfz6ye8 21 34 . . . cord-268467-btfz6ye8 22 1 They -PRON- PRP cord-268467-btfz6ye8 22 2 have have VBP cord-268467-btfz6ye8 22 3 also also RB cord-268467-btfz6ye8 22 4 been be VBN cord-268467-btfz6ye8 22 5 implicated implicate VBN cord-268467-btfz6ye8 22 6 in in IN cord-268467-btfz6ye8 22 7 gastrointestinal gastrointestinal JJ cord-268467-btfz6ye8 22 8 diseases disease NNS cord-268467-btfz6ye8 22 9 ( ( -LRB- cord-268467-btfz6ye8 22 10 Resta Resta NNP cord-268467-btfz6ye8 22 11 et et NNP cord-268467-btfz6ye8 22 12 a/. a/. NN cord-268467-btfz6ye8 22 13 , , , cord-268467-btfz6ye8 22 14 1985 1985 CD cord-268467-btfz6ye8 22 15 ) ) -RRB- cord-268467-btfz6ye8 22 16 . . . cord-268467-btfz6ye8 23 1 Furthermore furthermore RB cord-268467-btfz6ye8 23 2 , , , cord-268467-btfz6ye8 23 3 the the DT cord-268467-btfz6ye8 23 4 isolation isolation NN cord-268467-btfz6ye8 23 5 of of IN cord-268467-btfz6ye8 23 6 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 23 7 bearing bear VBG cord-268467-btfz6ye8 23 8 an an DT cord-268467-btfz6ye8 23 9 antigenic antigenic JJ cord-268467-btfz6ye8 23 10 relationship relationship NN cord-268467-btfz6ye8 23 11 to to IN cord-268467-btfz6ye8 23 12 HCV HCV NNP cord-268467-btfz6ye8 23 13 - - HYPH cord-268467-btfz6ye8 23 14 OC43 OC43 NNP cord-268467-btfz6ye8 23 15 from from IN cord-268467-btfz6ye8 23 16 the the DT cord-268467-btfz6ye8 23 17 central central JJ cord-268467-btfz6ye8 23 18 nervous nervous JJ cord-268467-btfz6ye8 23 19 system system NN cord-268467-btfz6ye8 23 20 of of IN cord-268467-btfz6ye8 23 21 two two CD cord-268467-btfz6ye8 23 22 patients patient NNS cord-268467-btfz6ye8 23 23 with with IN cord-268467-btfz6ye8 23 24 multiple multiple JJ cord-268467-btfz6ye8 23 25 sclerosis sclerosis NN cord-268467-btfz6ye8 23 26 has have VBZ cord-268467-btfz6ye8 23 27 suggested suggest VBN cord-268467-btfz6ye8 23 28 a a DT cord-268467-btfz6ye8 23 29 possible possible JJ cord-268467-btfz6ye8 23 30 etiologic etiologic JJ cord-268467-btfz6ye8 23 31 relationship relationship NN cord-268467-btfz6ye8 23 32 between between IN cord-268467-btfz6ye8 23 33 human human JJ cord-268467-btfz6ye8 23 34 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 23 35 and and CC cord-268467-btfz6ye8 23 36 multiple multiple JJ cord-268467-btfz6ye8 23 37 sclerosis sclerosis NN cord-268467-btfz6ye8 23 38 ( ( -LRB- cord-268467-btfz6ye8 23 39 Burks Burks NNP cord-268467-btfz6ye8 23 40 et et NNP cord-268467-btfz6ye8 23 41 a/. a/. NN cord-268467-btfz6ye8 23 42 , , , cord-268467-btfz6ye8 23 43 1980 1980 CD cord-268467-btfz6ye8 23 44 ) ) -RRB- cord-268467-btfz6ye8 23 45 . . . cord-268467-btfz6ye8 24 1 This this DT cord-268467-btfz6ye8 24 2 possibility possibility NN cord-268467-btfz6ye8 24 3 is be VBZ cord-268467-btfz6ye8 24 4 supported support VBN cord-268467-btfz6ye8 24 5 by by IN cord-268467-btfz6ye8 24 6 the the DT cord-268467-btfz6ye8 24 7 observation observation NN cord-268467-btfz6ye8 24 8 that that IN cord-268467-btfz6ye8 24 9 neurotropic neurotropic NNP cord-268467-btfz6ye8 24 10 strains strain NNS cord-268467-btfz6ye8 24 11 of of IN cord-268467-btfz6ye8 24 12 MHV MHV NNP cord-268467-btfz6ye8 24 13 cause cause VBP cord-268467-btfz6ye8 24 14 demyelination demyelination NN cord-268467-btfz6ye8 24 15 in in IN cord-268467-btfz6ye8 24 16 the the DT cord-268467-btfz6ye8 24 17 central central JJ cord-268467-btfz6ye8 24 18 nervous nervous JJ cord-268467-btfz6ye8 24 19 system system NN cord-268467-btfz6ye8 24 20 of of IN cord-268467-btfz6ye8 24 21 rodents rodent NNS cord-268467-btfz6ye8 24 22 ( ( -LRB- cord-268467-btfz6ye8 24 23 Weiner Weiner NNP cord-268467-btfz6ye8 24 24 and and CC cord-268467-btfz6ye8 24 25 Stohlman Stohlman NNP cord-268467-btfz6ye8 24 26 , , , cord-268467-btfz6ye8 24 27 1978 1978 CD cord-268467-btfz6ye8 24 28 ) ) -RRB- cord-268467-btfz6ye8 24 29 . . . cord-268467-btfz6ye8 25 1 Thus thus RB cord-268467-btfz6ye8 25 2 , , , cord-268467-btfz6ye8 25 3 human human JJ cord-268467-btfz6ye8 25 4 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 25 5 are be VBP cord-268467-btfz6ye8 25 6 important important JJ cord-268467-btfz6ye8 25 7 human human JJ cord-268467-btfz6ye8 25 8 pathogens pathogen NNS cord-268467-btfz6ye8 25 9 . . . cord-268467-btfz6ye8 26 1 The the DT cord-268467-btfz6ye8 26 2 structural structural JJ cord-268467-btfz6ye8 26 3 and and CC cord-268467-btfz6ye8 26 4 biochemical biochemical JJ cord-268467-btfz6ye8 26 5 properties property NNS cord-268467-btfz6ye8 26 6 of of IN cord-268467-btfz6ye8 26 7 several several JJ cord-268467-btfz6ye8 26 8 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 26 9 , , , cord-268467-btfz6ye8 26 10 particularly particularly RB cord-268467-btfz6ye8 26 11 MHV mhv NN cord-268467-btfz6ye8 26 12 and and CC cord-268467-btfz6ye8 26 13 avian avian JJ cord-268467-btfz6ye8 26 14 infectious infectious JJ cord-268467-btfz6ye8 26 15 Sequence sequence NN cord-268467-btfz6ye8 26 16 data datum NNS cord-268467-btfz6ye8 26 17 from from IN cord-268467-btfz6ye8 26 18 this this DT cord-268467-btfz6ye8 26 19 article article NN cord-268467-btfz6ye8 26 20 have have VBP cord-268467-btfz6ye8 26 21 been be VBN cord-268467-btfz6ye8 26 22 deposited deposit VBN cord-268467-btfz6ye8 26 23 with with IN cord-268467-btfz6ye8 26 24 the the DT cord-268467-btfz6ye8 26 25 EMBUGenBank EMBUGenBank NNP cord-268467-btfz6ye8 26 26 Data Data NNP cord-268467-btfz6ye8 26 27 Libraries Libraries NNPS cord-268467-btfz6ye8 26 28 under under IN cord-268467-btfz6ye8 27 1 Accession Accession NNP cord-268467-btfz6ye8 27 2 No no NN cord-268467-btfz6ye8 27 3 . . . cord-268467-btfz6ye8 28 1 JO441 JO441 NNP cord-268467-btfz6ye8 28 2 9 9 CD cord-268467-btfz6ye8 28 3 . . . cord-268467-btfz6ye8 28 4 ' ' '' cord-268467-btfz6ye8 29 1 To to TO cord-268467-btfz6ye8 29 2 whom whom WP cord-268467-btfz6ye8 29 3 requests request VBZ cord-268467-btfz6ye8 29 4 for for IN cord-268467-btfz6ye8 29 5 reprints reprint NNS cord-268467-btfz6ye8 29 6 should should MD cord-268467-btfz6ye8 29 7 be be VB cord-268467-btfz6ye8 29 8 addressed address VBN cord-268467-btfz6ye8 29 9 . . . cord-268467-btfz6ye8 30 1 2 2 LS cord-268467-btfz6ye8 31 1 Present present JJ cord-268467-btfz6ye8 31 2 address address NN cord-268467-btfz6ye8 31 3 : : : cord-268467-btfz6ye8 31 4 Department Department NNP cord-268467-btfz6ye8 31 5 of of IN cord-268467-btfz6ye8 31 6 Virology Virology NNP cord-268467-btfz6ye8 31 7 , , , cord-268467-btfz6ye8 31 8 Tottori Tottori NNP cord-268467-btfz6ye8 31 9 University University NNP cord-268467-btfz6ye8 31 10 , , , cord-268467-btfz6ye8 31 11 School School NNP cord-268467-btfz6ye8 31 12 of of IN cord-268467-btfz6ye8 31 13 Medicine Medicine NNP cord-268467-btfz6ye8 31 14 , , , cord-268467-btfz6ye8 31 15 Yonago Yonago NNP cord-268467-btfz6ye8 31 16 683 683 CD cord-268467-btfz6ye8 31 17 , , , cord-268467-btfz6ye8 31 18 Japan Japan NNP cord-268467-btfz6ye8 31 19 . . . cord-268467-btfz6ye8 32 1 peritonitis peritonitis NN cord-268467-btfz6ye8 32 2 virus virus NN cord-268467-btfz6ye8 32 3 ( ( -LRB- cord-268467-btfz6ye8 32 4 IBV IBV NNP cord-268467-btfz6ye8 32 5 ) ) -RRB- cord-268467-btfz6ye8 32 6 , , , cord-268467-btfz6ye8 32 7 have have VBP cord-268467-btfz6ye8 32 8 been be VBN cord-268467-btfz6ye8 32 9 well well RB cord-268467-btfz6ye8 32 10 characterized characterize VBN cord-268467-btfz6ye8 32 11 ( ( -LRB- cord-268467-btfz6ye8 32 12 Lai Lai NNP cord-268467-btfz6ye8 32 13 et et NNP cord-268467-btfz6ye8 32 14 a/. a/. NN cord-268467-btfz6ye8 32 15 , , , cord-268467-btfz6ye8 32 16 1987 1987 CD cord-268467-btfz6ye8 32 17 ; ; : cord-268467-btfz6ye8 32 18 Boursnell Boursnell NNP cord-268467-btfz6ye8 32 19 et et NNP cord-268467-btfz6ye8 32 20 a/. a/. NNP cord-268467-btfz6ye8 32 21 , , , cord-268467-btfz6ye8 32 22 1987 1987 CD cord-268467-btfz6ye8 32 23 ) ) -RRB- cord-268467-btfz6ye8 32 24 . . . cord-268467-btfz6ye8 33 1 The the DT cord-268467-btfz6ye8 33 2 virion virion NN cord-268467-btfz6ye8 33 3 contains contain VBZ cord-268467-btfz6ye8 33 4 a a DT cord-268467-btfz6ye8 33 5 single single RB cord-268467-btfz6ye8 33 6 - - HYPH cord-268467-btfz6ye8 33 7 stranded stranded JJ cord-268467-btfz6ye8 33 8 , , , cord-268467-btfz6ye8 33 9 positive positive JJ cord-268467-btfz6ye8 33 10 - - HYPH cord-268467-btfz6ye8 33 11 sense sense NN cord-268467-btfz6ye8 33 12 RNA RNA NNP cord-268467-btfz6ye8 33 13 molecule molecule NN cord-268467-btfz6ye8 33 14 ( ( -LRB- cord-268467-btfz6ye8 33 15 molecular molecular JJ cord-268467-btfz6ye8 33 16 weight weight NN cord-268467-btfz6ye8 33 17 6 6 CD cord-268467-btfz6ye8 33 18 - - SYM cord-268467-btfz6ye8 33 19 8 8 CD cord-268467-btfz6ye8 33 20 X x NN cord-268467-btfz6ye8 33 21 1 1 CD cord-268467-btfz6ye8 33 22 O6 O6 NNP cord-268467-btfz6ye8 33 23 Da Da NNP cord-268467-btfz6ye8 33 24 ) ) -RRB- cord-268467-btfz6ye8 33 25 ( ( -LRB- cord-268467-btfz6ye8 33 26 Lai Lai NNP cord-268467-btfz6ye8 33 27 and and CC cord-268467-btfz6ye8 33 28 Stohlman Stohlman NNP cord-268467-btfz6ye8 33 29 , , , cord-268467-btfz6ye8 33 30 1978 1978 CD cord-268467-btfz6ye8 33 31 ) ) -RRB- cord-268467-btfz6ye8 33 32 associated associate VBD cord-268467-btfz6ye8 33 33 in in IN cord-268467-btfz6ye8 33 34 a a DT cord-268467-btfz6ye8 33 35 helical helical JJ cord-268467-btfz6ye8 33 36 conformation conformation NN cord-268467-btfz6ye8 33 37 with with IN cord-268467-btfz6ye8 33 38 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 33 39 proteins protein NNS cord-268467-btfz6ye8 33 40 ( ( -LRB- cord-268467-btfz6ye8 33 41 N N NNP cord-268467-btfz6ye8 33 42 ) ) -RRB- cord-268467-btfz6ye8 33 43 . . . cord-268467-btfz6ye8 34 1 The the DT cord-268467-btfz6ye8 34 2 viral viral JJ cord-268467-btfz6ye8 34 3 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 34 4 is be VBZ cord-268467-btfz6ye8 34 5 enclosed enclose VBN cord-268467-btfz6ye8 34 6 by by IN cord-268467-btfz6ye8 34 7 an an DT cord-268467-btfz6ye8 34 8 envelope envelope NN cord-268467-btfz6ye8 34 9 , , , cord-268467-btfz6ye8 34 10 in in IN cord-268467-btfz6ye8 34 11 which which WDT cord-268467-btfz6ye8 34 12 are be VBP cord-268467-btfz6ye8 34 13 embedded embed VBN cord-268467-btfz6ye8 34 14 at at IN cord-268467-btfz6ye8 34 15 least least RBS cord-268467-btfz6ye8 34 16 two two CD cord-268467-btfz6ye8 34 17 types type NNS cord-268467-btfz6ye8 34 18 of of IN cord-268467-btfz6ye8 34 19 viral viral JJ cord-268467-btfz6ye8 34 20 proteins protein NNS cord-268467-btfz6ye8 34 21 , , , cord-268467-btfz6ye8 34 22 the the DT cord-268467-btfz6ye8 34 23 peplomer peplomer NNP cord-268467-btfz6ye8 34 24 ( ( -LRB- cord-268467-btfz6ye8 34 25 E2 E2 NNP cord-268467-btfz6ye8 34 26 ) ) -RRB- cord-268467-btfz6ye8 34 27 and and CC cord-268467-btfz6ye8 34 28 matrix matrix NN cord-268467-btfz6ye8 34 29 ( ( -LRB- cord-268467-btfz6ye8 34 30 El El NNP cord-268467-btfz6ye8 34 31 ) ) -RRB- cord-268467-btfz6ye8 34 32 glycoproteins glycoprotein NNS cord-268467-btfz6ye8 34 33 . . . cord-268467-btfz6ye8 35 1 Coronavirus Coronavirus NNP cord-268467-btfz6ye8 35 2 RNA RNA NNP cord-268467-btfz6ye8 35 3 replication replication NN cord-268467-btfz6ye8 35 4 occurs occur VBZ cord-268467-btfz6ye8 35 5 in in IN cord-268467-btfz6ye8 35 6 the the DT cord-268467-btfz6ye8 35 7 cytoplasm cytoplasm NN cord-268467-btfz6ye8 35 8 of of IN cord-268467-btfz6ye8 35 9 infected infected JJ cord-268467-btfz6ye8 35 10 cells cell NNS cord-268467-btfz6ye8 35 11 and and CC cord-268467-btfz6ye8 35 12 is be VBZ cord-268467-btfz6ye8 35 13 mediated mediate VBN cord-268467-btfz6ye8 35 14 by by IN cord-268467-btfz6ye8 35 15 a a DT cord-268467-btfz6ye8 35 16 virusencoded virusencode VBN cord-268467-btfz6ye8 35 17 RNA RNA NNP cord-268467-btfz6ye8 35 18 - - HYPH cord-268467-btfz6ye8 35 19 dependent dependent JJ cord-268467-btfz6ye8 35 20 RNA RNA NNP cord-268467-btfz6ye8 35 21 polymerase polymerase NN cord-268467-btfz6ye8 35 22 ( ( -LRB- cord-268467-btfz6ye8 35 23 Brayton Brayton NNP cord-268467-btfz6ye8 35 24 et et NNP cord-268467-btfz6ye8 35 25 a/. a/. NNP cord-268467-btfz6ye8 35 26 , , , cord-268467-btfz6ye8 35 27 1982 1982 CD cord-268467-btfz6ye8 35 28 ) ) -RRB- cord-268467-btfz6ye8 35 29 . . . cord-268467-btfz6ye8 36 1 The the DT cord-268467-btfz6ye8 36 2 virus virus NN cord-268467-btfz6ye8 36 3 - - HYPH cord-268467-btfz6ye8 36 4 specific specific JJ cord-268467-btfz6ye8 36 5 mRNA mrna NN cord-268467-btfz6ye8 36 6 in in IN cord-268467-btfz6ye8 36 7 infected infected JJ cord-268467-btfz6ye8 36 8 cells cell NNS cord-268467-btfz6ye8 36 9 comprises comprise VBZ cord-268467-btfz6ye8 36 10 a a DT cord-268467-btfz6ye8 36 11 genomic genomic NN cord-268467-btfz6ye8 36 12 - - HYPH cord-268467-btfz6ye8 36 13 sized sized JJ cord-268467-btfz6ye8 36 14 RNA RNA NNP cord-268467-btfz6ye8 36 15 plus plus CC cord-268467-btfz6ye8 36 16 six six CD cord-268467-btfz6ye8 36 17 subgenomic subgenomic NNP cord-268467-btfz6ye8 36 18 mRNA mRNA NNP cord-268467-btfz6ye8 36 19 species specie NNS cord-268467-btfz6ye8 36 20 . . . cord-268467-btfz6ye8 37 1 These these DT cord-268467-btfz6ye8 37 2 mRNAs mrna NNS cord-268467-btfz6ye8 37 3 are be VBP cord-268467-btfz6ye8 37 4 arranged arrange VBN cord-268467-btfz6ye8 37 5 in in IN cord-268467-btfz6ye8 37 6 a a DT cord-268467-btfz6ye8 37 7 nested nest VBN cord-268467-btfz6ye8 37 8 - - HYPH cord-268467-btfz6ye8 37 9 set set VBN cord-268467-btfz6ye8 37 10 structure structure NN cord-268467-btfz6ye8 37 11 , , , cord-268467-btfz6ye8 37 12 which which WDT cord-268467-btfz6ye8 37 13 is be VBZ cord-268467-btfz6ye8 37 14 characterized characterize VBN cord-268467-btfz6ye8 37 15 by by IN cord-268467-btfz6ye8 37 16 RNAs rna NNS cord-268467-btfz6ye8 37 17 having have VBG cord-268467-btfz6ye8 37 18 common common JJ cord-268467-btfz6ye8 37 19 3'-termini 3'-termini CD cord-268467-btfz6ye8 37 20 but but CC cord-268467-btfz6ye8 37 21 extending extend VBG cord-268467-btfz6ye8 37 22 for for IN cord-268467-btfz6ye8 37 23 varying vary VBG cord-268467-btfz6ye8 37 24 lengths length NNS cord-268467-btfz6ye8 37 25 in in IN cord-268467-btfz6ye8 37 26 the the DT cord-268467-btfz6ye8 37 27 5'direction 5'direction CD cord-268467-btfz6ye8 38 1 ( ( -LRB- cord-268467-btfz6ye8 38 2 Lai Lai NNP cord-268467-btfz6ye8 38 3 et et FW cord-268467-btfz6ye8 38 4 al al NNP cord-268467-btfz6ye8 38 5 . . NNP cord-268467-btfz6ye8 38 6 , , , cord-268467-btfz6ye8 38 7 1981 1981 CD cord-268467-btfz6ye8 38 8 ) ) -RRB- cord-268467-btfz6ye8 38 9 . . . cord-268467-btfz6ye8 39 1 Only only RB cord-268467-btfz6ye8 39 2 the the DT cord-268467-btfz6ye8 39 3 5'proximal 5'proximal CD cord-268467-btfz6ye8 39 4 regions region NNS cord-268467-btfz6ye8 39 5 of of IN cord-268467-btfz6ye8 39 6 each each DT cord-268467-btfz6ye8 39 7 mRNA mRNA NNP cord-268467-btfz6ye8 39 8 are be VBP cord-268467-btfz6ye8 39 9 translated translate VBN cord-268467-btfz6ye8 40 1 ( ( -LRB- cord-268467-btfz6ye8 40 2 Rottier Rottier NNP cord-268467-btfz6ye8 40 3 et et NNP cord-268467-btfz6ye8 40 4 a/. a/. NN cord-268467-btfz6ye8 40 5 , , , cord-268467-btfz6ye8 40 6 1981 1981 CD cord-268467-btfz6ye8 40 7 ) ) -RRB- cord-268467-btfz6ye8 40 8 . . . cord-268467-btfz6ye8 41 1 A a DT cord-268467-btfz6ye8 41 2 unique unique JJ cord-268467-btfz6ye8 41 3 feature feature NN cord-268467-btfz6ye8 41 4 of of IN cord-268467-btfz6ye8 41 5 the the DT cord-268467-btfz6ye8 41 6 structure structure NN cord-268467-btfz6ye8 41 7 of of IN cord-268467-btfz6ye8 41 8 coronavirus coronavirus NN cord-268467-btfz6ye8 41 9 is be VBZ cord-268467-btfz6ye8 41 10 the the DT cord-268467-btfz6ye8 41 11 existence existence NN cord-268467-btfz6ye8 41 12 , , , cord-268467-btfz6ye8 41 13 at at IN cord-268467-btfz6ye8 41 14 the the DT cord-268467-btfz6ye8 41 15 5'-end 5'-end CD cord-268467-btfz6ye8 41 16 of of IN cord-268467-btfz6ye8 41 17 each each DT cord-268467-btfz6ye8 41 18 mRNA mrna NN cord-268467-btfz6ye8 41 19 , , , cord-268467-btfz6ye8 41 20 of of IN cord-268467-btfz6ye8 41 21 an an DT cord-268467-btfz6ye8 41 22 identical identical JJ cord-268467-btfz6ye8 41 23 leader leader NN cord-268467-btfz6ye8 41 24 sequence sequence NN cord-268467-btfz6ye8 41 25 . . . cord-268467-btfz6ye8 42 1 This this DT cord-268467-btfz6ye8 42 2 sequence sequence NN cord-268467-btfz6ye8 42 3 is be VBZ cord-268467-btfz6ye8 42 4 derived derive VBN cord-268467-btfz6ye8 42 5 from from IN cord-268467-btfz6ye8 42 6 the the DT cord-268467-btfz6ye8 42 7 5'-end 5'-end CD cord-268467-btfz6ye8 42 8 of of IN cord-268467-btfz6ye8 42 9 the the DT cord-268467-btfz6ye8 42 10 genomic genomic JJ cord-268467-btfz6ye8 42 11 RNA RNA NNP cord-268467-btfz6ye8 42 12 and and CC cord-268467-btfz6ye8 42 13 is be VBZ cord-268467-btfz6ye8 42 14 of of IN cord-268467-btfz6ye8 42 15 approximately approximately RB cord-268467-btfz6ye8 42 16 70 70 CD cord-268467-btfz6ye8 42 17 nucleotides nucleotide NNS cord-268467-btfz6ye8 42 18 in in IN cord-268467-btfz6ye8 42 19 length length NN cord-268467-btfz6ye8 43 1 ( ( -LRB- cord-268467-btfz6ye8 43 2 Lai Lai NNP cord-268467-btfz6ye8 43 3 eta/. eta/. CD cord-268467-btfz6ye8 43 4 , , , cord-268467-btfz6ye8 43 5 1983 1983 CD cord-268467-btfz6ye8 43 6 ( ( -LRB- cord-268467-btfz6ye8 43 7 Lai Lai NNP cord-268467-btfz6ye8 43 8 eta/. eta/. NNP cord-268467-btfz6ye8 43 9 , , , cord-268467-btfz6ye8 43 10 , , , cord-268467-btfz6ye8 43 11 1984 1984 CD cord-268467-btfz6ye8 43 12 . . . cord-268467-btfz6ye8 44 1 Recent recent JJ cord-268467-btfz6ye8 44 2 evidence evidence NN cord-268467-btfz6ye8 44 3 has have VBZ cord-268467-btfz6ye8 44 4 supported support VBN cord-268467-btfz6ye8 44 5 a a DT cord-268467-btfz6ye8 44 6 role role NN cord-268467-btfz6ye8 44 7 for for IN cord-268467-btfz6ye8 44 8 the the DT cord-268467-btfz6ye8 44 9 leader leader NN cord-268467-btfz6ye8 44 10 sequence sequence NN cord-268467-btfz6ye8 44 11 in in IN cord-268467-btfz6ye8 44 12 mediating mediate VBG cord-268467-btfz6ye8 44 13 a a DT cord-268467-btfz6ye8 44 14 novel novel JJ cord-268467-btfz6ye8 44 15 type type NN cord-268467-btfz6ye8 44 16 of of IN cord-268467-btfz6ye8 44 17 discontinuous discontinuous JJ cord-268467-btfz6ye8 44 18 transcription transcription NN cord-268467-btfz6ye8 44 19 of of IN cord-268467-btfz6ye8 44 20 genomic genomic JJ cord-268467-btfz6ye8 44 21 RNA RNA NNP cord-268467-btfz6ye8 45 1 ( ( -LRB- cord-268467-btfz6ye8 45 2 Baric Baric NNP cord-268467-btfz6ye8 45 3 et et NNP cord-268467-btfz6ye8 45 4 a/. a/. NN cord-268467-btfz6ye8 45 5 , , , cord-268467-btfz6ye8 45 6 1985 1985 CD cord-268467-btfz6ye8 45 7 ; ; : cord-268467-btfz6ye8 46 1 Makino Makino NNP cord-268467-btfz6ye8 46 2 et et NNP cord-268467-btfz6ye8 46 3 al al NNP cord-268467-btfz6ye8 46 4 . . NNP cord-268467-btfz6ye8 46 5 , , , cord-268467-btfz6ye8 46 6 1986 1986 CD cord-268467-btfz6ye8 46 7 ; ; : cord-268467-btfz6ye8 46 8 . . . cord-268467-btfz6ye8 47 1 In in IN cord-268467-btfz6ye8 47 2 contrast contrast NN cord-268467-btfz6ye8 47 3 to to IN cord-268467-btfz6ye8 47 4 other other JJ cord-268467-btfz6ye8 47 5 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 47 6 , , , cord-268467-btfz6ye8 47 7 the the DT cord-268467-btfz6ye8 47 8 molecular molecular JJ cord-268467-btfz6ye8 47 9 biology biology NN cord-268467-btfz6ye8 47 10 of of IN cord-268467-btfz6ye8 47 11 human human JJ cord-268467-btfz6ye8 47 12 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 47 13 is be VBZ cord-268467-btfz6ye8 47 14 relatively relatively RB cord-268467-btfz6ye8 47 15 poorly poorly RB cord-268467-btfz6ye8 47 16 understood understand VBN cord-268467-btfz6ye8 47 17 . . . cord-268467-btfz6ye8 48 1 The the DT cord-268467-btfz6ye8 48 2 genomic genomic JJ cord-268467-btfz6ye8 48 3 RNA rna NN cord-268467-btfz6ye8 48 4 of of IN cord-268467-btfz6ye8 48 5 both both DT cord-268467-btfz6ye8 49 1 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 49 2 and and CC cord-268467-btfz6ye8 49 3 HCV HCV NNP cord-268467-btfz6ye8 49 4 - - HYPH cord-268467-btfz6ye8 49 5 OC43 OC43 NNP cord-268467-btfz6ye8 49 6 has have VBZ cord-268467-btfz6ye8 49 7 a a DT cord-268467-btfz6ye8 49 8 molecular molecular JJ cord-268467-btfz6ye8 49 9 weight weight NN cord-268467-btfz6ye8 49 10 of of IN cord-268467-btfz6ye8 49 11 approximately approximately RB cord-268467-btfz6ye8 49 12 6 6 CD cord-268467-btfz6ye8 49 13 x x SYM cord-268467-btfz6ye8 49 14 1 1 CD cord-268467-btfz6ye8 49 15 O6 O6 NNP cord-268467-btfz6ye8 49 16 Da Da NNP cord-268467-btfz6ye8 49 17 ( ( -LRB- cord-268467-btfz6ye8 49 18 Hierholzer Hierholzer NNP cord-268467-btfz6ye8 49 19 et et NNP cord-268467-btfz6ye8 49 20 al al NNP cord-268467-btfz6ye8 49 21 . . NNP cord-268467-btfz6ye8 49 22 , , , cord-268467-btfz6ye8 49 23 1981 1981 CD cord-268467-btfz6ye8 49 24 ) ) -RRB- cord-268467-btfz6ye8 49 25 . . . cord-268467-btfz6ye8 50 1 The the DT cord-268467-btfz6ye8 50 2 six six CD cord-268467-btfz6ye8 50 3 subgenomic subgenomic NNP cord-268467-btfz6ye8 50 4 RNA RNA NNP cord-268467-btfz6ye8 50 5 species specie NNS cord-268467-btfz6ye8 50 6 appear appear VBP cord-268467-btfz6ye8 50 7 to to TO cord-268467-btfz6ye8 50 8 have have VB cord-268467-btfz6ye8 50 9 lower low JJR cord-268467-btfz6ye8 50 10 molecular molecular JJ cord-268467-btfz6ye8 50 11 weights weight NNS cord-268467-btfz6ye8 50 12 than than IN cord-268467-btfz6ye8 50 13 those those DT cord-268467-btfz6ye8 50 14 of of IN cord-268467-btfz6ye8 50 15 the the DT cord-268467-btfz6ye8 50 16 corresponding corresponding JJ cord-268467-btfz6ye8 50 17 MHV MHV NNP cord-268467-btfz6ye8 50 18 RNAs rna NNS cord-268467-btfz6ye8 50 19 ( ( -LRB- cord-268467-btfz6ye8 50 20 Weiss Weiss NNP cord-268467-btfz6ye8 50 21 and and CC cord-268467-btfz6ye8 50 22 Leibowitz Leibowitz NNP cord-268467-btfz6ye8 50 23 , , , cord-268467-btfz6ye8 50 24 198 198 CD cord-268467-btfz6ye8 50 25 1 1 CD cord-268467-btfz6ye8 50 26 ) ) -RRB- cord-268467-btfz6ye8 50 27 . . . cord-268467-btfz6ye8 51 1 The the DT cord-268467-btfz6ye8 51 2 structure structure NN cord-268467-btfz6ye8 51 3 of of IN cord-268467-btfz6ye8 51 4 these these DT cord-268467-btfz6ye8 51 5 mRNAs mRNAs NNPS cord-268467-btfz6ye8 51 6 is be VBZ cord-268467-btfz6ye8 51 7 not not RB cord-268467-btfz6ye8 51 8 yet yet RB cord-268467-btfz6ye8 51 9 known know VBN cord-268467-btfz6ye8 51 10 . . . cord-268467-btfz6ye8 52 1 Analysis analysis NN cord-268467-btfz6ye8 52 2 of of IN cord-268467-btfz6ye8 52 3 purified purify VBN cord-268467-btfz6ye8 52 4 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 52 5 virions virion NNS cord-268467-btfz6ye8 52 6 has have VBZ cord-268467-btfz6ye8 52 7 revealed reveal VBN cord-268467-btfz6ye8 52 8 three three CD cord-268467-btfz6ye8 52 9 major major JJ cord-268467-btfz6ye8 52 10 polypeptides polypeptide NNS cord-268467-btfz6ye8 52 11 : : : cord-268467-btfz6ye8 52 12 a a DT cord-268467-btfz6ye8 52 13 glycosylated glycosylated JJ cord-268467-btfz6ye8 52 14 protein protein NN cord-268467-btfz6ye8 52 15 with with IN cord-268467-btfz6ye8 52 16 a a DT cord-268467-btfz6ye8 52 17 molecular molecular JJ cord-268467-btfz6ye8 52 18 weight weight NN cord-268467-btfz6ye8 52 19 of of IN cord-268467-btfz6ye8 52 20 180 180 CD cord-268467-btfz6ye8 52 21 kDa kDa NNPS cord-268467-btfz6ye8 52 22 , , , cord-268467-btfz6ye8 52 23 a a DT cord-268467-btfz6ye8 52 24 phosphorylated phosphorylated JJ cord-268467-btfz6ye8 52 25 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 52 26 protein protein NN cord-268467-btfz6ye8 52 27 of of IN cord-268467-btfz6ye8 52 28 50 50 CD cord-268467-btfz6ye8 52 29 kDa kDa NNS cord-268467-btfz6ye8 52 30 , , , cord-268467-btfz6ye8 52 31 and and CC cord-268467-btfz6ye8 52 32 a a DT cord-268467-btfz6ye8 52 33 family family NN cord-268467-btfz6ye8 52 34 of of IN cord-268467-btfz6ye8 52 35 polypeptides polypeptide NNS cord-268467-btfz6ye8 52 36 with with IN cord-268467-btfz6ye8 52 37 molecular molecular JJ cord-268467-btfz6ye8 52 38 weights weight NNS cord-268467-btfz6ye8 52 39 of of IN cord-268467-btfz6ye8 52 40 25 25 CD cord-268467-btfz6ye8 52 41 , , , cord-268467-btfz6ye8 52 42 23 23 CD cord-268467-btfz6ye8 52 43 , , , cord-268467-btfz6ye8 52 44 and and CC cord-268467-btfz6ye8 52 45 21 21 CD cord-268467-btfz6ye8 52 46 kDa kDa NNS cord-268467-btfz6ye8 52 47 ( ( -LRB- cord-268467-btfz6ye8 52 48 Kemp Kemp NNP cord-268467-btfz6ye8 52 49 et et NNP cord-268467-btfz6ye8 52 50 al al NNP cord-268467-btfz6ye8 52 51 . . NNP cord-268467-btfz6ye8 52 52 , , , cord-268467-btfz6ye8 52 53 1984 1984 CD cord-268467-btfz6ye8 52 54 ) ) -RRB- cord-268467-btfz6ye8 52 55 . . . cord-268467-btfz6ye8 53 1 In in IN cord-268467-btfz6ye8 53 2 addition addition NN cord-268467-btfz6ye8 53 3 , , , cord-268467-btfz6ye8 53 4 several several JJ cord-268467-btfz6ye8 53 5 minor minor JJ cord-268467-btfz6ye8 53 6 nonstructural nonstructural JJ cord-268467-btfz6ye8 53 7 polypeptides polypeptide NNS cord-268467-btfz6ye8 53 8 of of IN cord-268467-btfz6ye8 53 9 107 107 CD cord-268467-btfz6ye8 53 10 , , , cord-268467-btfz6ye8 53 11 92 92 CD cord-268467-btfz6ye8 53 12 , , , cord-268467-btfz6ye8 53 13 and and CC cord-268467-btfz6ye8 53 14 39 39 CD cord-268467-btfz6ye8 53 15 kDa kDa NNS cord-268467-btfz6ye8 53 16 have have VBP cord-268467-btfz6ye8 53 17 been be VBN cord-268467-btfz6ye8 53 18 identified identify VBN cord-268467-btfz6ye8 53 19 ( ( -LRB- cord-268467-btfz6ye8 53 20 Kemp Kemp NNP cord-268467-btfz6ye8 53 21 et et NNP cord-268467-btfz6ye8 53 22 al al NNP cord-268467-btfz6ye8 53 23 . . NNP cord-268467-btfz6ye8 53 24 , , , cord-268467-btfz6ye8 53 25 1984 1984 CD cord-268467-btfz6ye8 53 26 ) ) -RRB- cord-268467-btfz6ye8 53 27 . . . cord-268467-btfz6ye8 54 1 The the DT cord-268467-btfz6ye8 54 2 functions function NNS cord-268467-btfz6ye8 54 3 of of IN cord-268467-btfz6ye8 54 4 these these DT cord-268467-btfz6ye8 54 5 proteins protein NNS cord-268467-btfz6ye8 54 6 have have VBP cord-268467-btfz6ye8 54 7 not not RB cord-268467-btfz6ye8 54 8 yet yet RB cord-268467-btfz6ye8 54 9 been be VBN cord-268467-btfz6ye8 54 10 characterized characterize VBN cord-268467-btfz6ye8 54 11 . . . cord-268467-btfz6ye8 55 1 To to TO cord-268467-btfz6ye8 55 2 further further RB cord-268467-btfz6ye8 55 3 understand understand VB cord-268467-btfz6ye8 55 4 the the DT cord-268467-btfz6ye8 55 5 molecular molecular JJ cord-268467-btfz6ye8 55 6 biology biology NN cord-268467-btfz6ye8 55 7 of of IN cord-268467-btfz6ye8 55 8 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 55 9 , , , cord-268467-btfz6ye8 55 10 we -PRON- PRP cord-268467-btfz6ye8 55 11 have have VBP cord-268467-btfz6ye8 55 12 initiated initiate VBN cord-268467-btfz6ye8 55 13 molecular molecular JJ cord-268467-btfz6ye8 55 14 cloning cloning NN cord-268467-btfz6ye8 55 15 and and CC cord-268467-btfz6ye8 55 16 sequence sequence NN cord-268467-btfz6ye8 55 17 analysis analysis NN cord-268467-btfz6ye8 55 18 of of IN cord-268467-btfz6ye8 55 19 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 55 20 RNA RNA NNP cord-268467-btfz6ye8 55 21 . . . cord-268467-btfz6ye8 56 1 In in IN cord-268467-btfz6ye8 56 2 this this DT cord-268467-btfz6ye8 56 3 paper paper NN cord-268467-btfz6ye8 56 4 we -PRON- PRP cord-268467-btfz6ye8 56 5 report report VBP cord-268467-btfz6ye8 56 6 the the DT cord-268467-btfz6ye8 56 7 sequence sequence NN cord-268467-btfz6ye8 56 8 analysis analysis NN cord-268467-btfz6ye8 56 9 of of IN cord-268467-btfz6ye8 56 10 the the DT cord-268467-btfz6ye8 56 11 gene gene NN cord-268467-btfz6ye8 56 12 encoding encode VBG cord-268467-btfz6ye8 56 13 the the DT cord-268467-btfz6ye8 56 14 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 56 15 protein protein NN cord-268467-btfz6ye8 56 16 of of IN cord-268467-btfz6ye8 56 17 HCV-229E. HCV-229E. NNP cord-268467-btfz6ye8 57 1 In in IN cord-268467-btfz6ye8 57 2 addition addition NN cord-268467-btfz6ye8 57 3 , , , cord-268467-btfz6ye8 57 4 the the DT cord-268467-btfz6ye8 57 5 mRNA mRNA NNP cord-268467-btfz6ye8 57 6 leader leader NN cord-268467-btfz6ye8 57 7 sequence sequence NN cord-268467-btfz6ye8 57 8 was be VBD cord-268467-btfz6ye8 57 9 also also RB cord-268467-btfz6ye8 57 10 identified identify VBN cord-268467-btfz6ye8 57 11 . . . cord-268467-btfz6ye8 58 1 The the DT cord-268467-btfz6ye8 58 2 results result NNS cord-268467-btfz6ye8 58 3 are be VBP cord-268467-btfz6ye8 58 4 compared compare VBN cord-268467-btfz6ye8 58 5 with with IN cord-268467-btfz6ye8 58 6 sequences sequence NNS cord-268467-btfz6ye8 58 7 of of IN cord-268467-btfz6ye8 58 8 other other JJ cord-268467-btfz6ye8 58 9 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 58 10 including include VBG cord-268467-btfz6ye8 58 11 MHV MHV NNP cord-268467-btfz6ye8 58 12 , , , cord-268467-btfz6ye8 58 13 BCV BCV NNP cord-268467-btfz6ye8 58 14 , , , cord-268467-btfz6ye8 58 15 IBV IBV NNP cord-268467-btfz6ye8 58 16 , , , cord-268467-btfz6ye8 58 17 and and CC cord-268467-btfz6ye8 58 18 TGEV TGEV NNP cord-268467-btfz6ye8 58 19 . . . cord-268467-btfz6ye8 59 1 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 59 2 ( ( -LRB- cord-268467-btfz6ye8 59 3 obtained obtain VBN cord-268467-btfz6ye8 59 4 from from IN cord-268467-btfz6ye8 59 5 Dr. Dr. NNP cord-268467-btfz6ye8 59 6 J. J. NNP cord-268467-btfz6ye8 59 7 Fleming Fleming NNP cord-268467-btfz6ye8 59 8 , , , cord-268467-btfz6ye8 59 9 University University NNP cord-268467-btfz6ye8 59 10 of of IN cord-268467-btfz6ye8 59 11 Southern Southern NNP cord-268467-btfz6ye8 59 12 California California NNP cord-268467-btfz6ye8 59 13 ) ) -RRB- cord-268467-btfz6ye8 59 14 was be VBD cord-268467-btfz6ye8 59 15 propagated propagate VBN cord-268467-btfz6ye8 59 16 at at IN cord-268467-btfz6ye8 59 17 low low JJ cord-268467-btfz6ye8 59 18 multiplicities multiplicity NNS cord-268467-btfz6ye8 59 19 of of IN cord-268467-btfz6ye8 59 20 infection infection NN cord-268467-btfz6ye8 59 21 in in IN cord-268467-btfz6ye8 59 22 human human JJ cord-268467-btfz6ye8 59 23 fetal fetal JJ cord-268467-btfz6ye8 59 24 lung lung NN cord-268467-btfz6ye8 59 25 cells cell NNS cord-268467-btfz6ye8 59 26 L132 L132 . cord-268467-btfz6ye8 60 1 ( ( -LRB- cord-268467-btfz6ye8 60 2 Kennedy Kennedy NNP cord-268467-btfz6ye8 60 3 and and CC cord-268467-btfz6ye8 60 4 Johnson Johnson NNP cord-268467-btfz6ye8 60 5 - - HYPH cord-268467-btfz6ye8 60 6 Lussenberg Lussenberg NNP cord-268467-btfz6ye8 60 7 , , , cord-268467-btfz6ye8 60 8 197511976 197511976 CD cord-268467-btfz6ye8 60 9 ) ) -RRB- cord-268467-btfz6ye8 60 10 using use VBG cord-268467-btfz6ye8 60 11 Dulbecco Dulbecco NNP cord-268467-btfz6ye8 60 12 's 's POS cord-268467-btfz6ye8 60 13 modified modify VBN cord-268467-btfz6ye8 60 14 Eagle Eagle NNP cord-268467-btfz6ye8 60 15 's 's POS cord-268467-btfz6ye8 60 16 medium medium NN cord-268467-btfz6ye8 60 17 ( ( -LRB- cord-268467-btfz6ye8 60 18 DMEM DMEM NNP cord-268467-btfz6ye8 60 19 ) ) -RRB- cord-268467-btfz6ye8 60 20 supplemented supplement VBD cord-268467-btfz6ye8 60 21 with with IN cord-268467-btfz6ye8 60 22 10 10 CD cord-268467-btfz6ye8 60 23 % % NN cord-268467-btfz6ye8 60 24 fetal fetal JJ cord-268467-btfz6ye8 60 25 calf calf NN cord-268467-btfz6ye8 60 26 serum serum NN cord-268467-btfz6ye8 60 27 . . . cord-268467-btfz6ye8 61 1 Virus virus NN cord-268467-btfz6ye8 61 2 purification purification NN cord-268467-btfz6ye8 61 3 and and CC cord-268467-btfz6ye8 61 4 preparation preparation NN cord-268467-btfz6ye8 61 5 of of IN cord-268467-btfz6ye8 61 6 virion virion NN cord-268467-btfz6ye8 61 7 RNA rna NN cord-268467-btfz6ye8 61 8 Following follow VBG cord-268467-btfz6ye8 61 9 a a DT cord-268467-btfz6ye8 61 10 virus virus NN cord-268467-btfz6ye8 61 11 adsorption adsorption NN cord-268467-btfz6ye8 61 12 period period NN cord-268467-btfz6ye8 61 13 of of IN cord-268467-btfz6ye8 61 14 1 1 CD cord-268467-btfz6ye8 61 15 hr hr NN cord-268467-btfz6ye8 61 16 at at IN cord-268467-btfz6ye8 61 17 37 37 CD cord-268467-btfz6ye8 61 18 " " '' cord-268467-btfz6ye8 61 19 , , , cord-268467-btfz6ye8 61 20 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 61 21 - - HYPH cord-268467-btfz6ye8 61 22 infected infect VBN cord-268467-btfz6ye8 61 23 L132 L132 NNP cord-268467-btfz6ye8 61 24 monolayers monolayer NNS cord-268467-btfz6ye8 61 25 were be VBD cord-268467-btfz6ye8 61 26 incubated incubate VBN cord-268467-btfz6ye8 61 27 at at IN cord-268467-btfz6ye8 61 28 37 37 CD cord-268467-btfz6ye8 61 29 " " `` cord-268467-btfz6ye8 61 30 for for IN cord-268467-btfz6ye8 61 31 24 24 CD cord-268467-btfz6ye8 61 32 to to TO cord-268467-btfz6ye8 61 33 48 48 CD cord-268467-btfz6ye8 61 34 hr hr NN cord-268467-btfz6ye8 61 35 , , , cord-268467-btfz6ye8 61 36 at at IN cord-268467-btfz6ye8 61 37 which which WDT cord-268467-btfz6ye8 61 38 time time NN cord-268467-btfz6ye8 61 39 the the DT cord-268467-btfz6ye8 61 40 cell cell NN cord-268467-btfz6ye8 61 41 culture culture NN cord-268467-btfz6ye8 61 42 fluid fluid NN cord-268467-btfz6ye8 61 43 was be VBD cord-268467-btfz6ye8 61 44 harvested harvest VBN cord-268467-btfz6ye8 61 45 . . . cord-268467-btfz6ye8 62 1 Viruses virus NNS cord-268467-btfz6ye8 62 2 were be VBD cord-268467-btfz6ye8 62 3 precipitated precipitate VBN cord-268467-btfz6ye8 62 4 from from IN cord-268467-btfz6ye8 62 5 2 2 CD cord-268467-btfz6ye8 62 6 liters liter NNS cord-268467-btfz6ye8 62 7 of of IN cord-268467-btfz6ye8 62 8 culture culture NN cord-268467-btfz6ye8 62 9 fluid fluid NN cord-268467-btfz6ye8 62 10 with with IN cord-268467-btfz6ye8 62 11 50 50 CD cord-268467-btfz6ye8 62 12 % % NN cord-268467-btfz6ye8 62 13 ammonium ammonium NN cord-268467-btfz6ye8 62 14 sulfate sulfate NN cord-268467-btfz6ye8 62 15 and and CC cord-268467-btfz6ye8 62 16 centrifuged centrifuge VBD cord-268467-btfz6ye8 62 17 at at IN cord-268467-btfz6ye8 62 18 8000 8000 CD cord-268467-btfz6ye8 62 19 rpm rpm NN cord-268467-btfz6ye8 62 20 for for IN cord-268467-btfz6ye8 62 21 30 30 CD cord-268467-btfz6ye8 62 22 min min NN cord-268467-btfz6ye8 62 23 . . . cord-268467-btfz6ye8 63 1 The the DT cord-268467-btfz6ye8 63 2 pellet pellet NN cord-268467-btfz6ye8 63 3 was be VBD cord-268467-btfz6ye8 63 4 resuspended resuspend VBN cord-268467-btfz6ye8 63 5 in in IN cord-268467-btfz6ye8 63 6 NTE NTE NNP cord-268467-btfz6ye8 63 7 buffer buffer NN cord-268467-btfz6ye8 63 8 ( ( -LRB- cord-268467-btfz6ye8 63 9 0.1 0.1 CD cord-268467-btfz6ye8 63 10 M M NNP cord-268467-btfz6ye8 63 11 NaCI NaCI NNP cord-268467-btfz6ye8 63 12 , , , cord-268467-btfz6ye8 63 13 0.01 0.01 CD cord-268467-btfz6ye8 63 14 M M NNP cord-268467-btfz6ye8 63 15 Tris Tris NNP cord-268467-btfz6ye8 63 16 - - HYPH cord-268467-btfz6ye8 63 17 hydrochloride hydrochloride NNP cord-268467-btfz6ye8 63 18 ( ( -LRB- cord-268467-btfz6ye8 63 19 pH pH NNP cord-268467-btfz6ye8 63 20 7.2 7.2 CD cord-268467-btfz6ye8 63 21 ) ) -RRB- cord-268467-btfz6ye8 63 22 , , , cord-268467-btfz6ye8 63 23 1 1 CD cord-268467-btfz6ye8 63 24 mM mm CD cord-268467-btfz6ye8 63 25 EDTA EDTA NNP cord-268467-btfz6ye8 63 26 ) ) -RRB- cord-268467-btfz6ye8 63 27 and and CC cord-268467-btfz6ye8 63 28 then then RB cord-268467-btfz6ye8 63 29 placed place VBD cord-268467-btfz6ye8 63 30 on on IN cord-268467-btfz6ye8 63 31 a a DT cord-268467-btfz6ye8 63 32 discontinuous discontinuous JJ cord-268467-btfz6ye8 63 33 sucrose sucrose NN cord-268467-btfz6ye8 63 34 gradient gradient NN cord-268467-btfz6ye8 63 35 consisting consist VBG cord-268467-btfz6ye8 63 36 of of IN cord-268467-btfz6ye8 63 37 60 60 CD cord-268467-btfz6ye8 63 38 , , , cord-268467-btfz6ye8 63 39 50 50 CD cord-268467-btfz6ye8 63 40 , , , cord-268467-btfz6ye8 63 41 30 30 CD cord-268467-btfz6ye8 63 42 , , , cord-268467-btfz6ye8 63 43 and and CC cord-268467-btfz6ye8 63 44 20 20 CD cord-268467-btfz6ye8 63 45 % % NN cord-268467-btfz6ye8 63 46 ( ( -LRB- cord-268467-btfz6ye8 63 47 w w NNP cord-268467-btfz6ye8 63 48 / / SYM cord-268467-btfz6ye8 63 49 w w NN cord-268467-btfz6ye8 63 50 ) ) -RRB- cord-268467-btfz6ye8 63 51 sucrose sucrose NN cord-268467-btfz6ye8 63 52 in in IN cord-268467-btfz6ye8 63 53 NTE NTE NNP cord-268467-btfz6ye8 63 54 buffer buffer NN cord-268467-btfz6ye8 63 55 and and CC cord-268467-btfz6ye8 63 56 centrifuged centrifuge VBN cord-268467-btfz6ye8 63 57 at at IN cord-268467-btfz6ye8 63 58 26,000 26,000 CD cord-268467-btfz6ye8 63 59 rpm rpm NN cord-268467-btfz6ye8 63 60 for for IN cord-268467-btfz6ye8 63 61 13 13 CD cord-268467-btfz6ye8 63 62 hr hr NN cord-268467-btfz6ye8 63 63 at at IN cord-268467-btfz6ye8 63 64 4 4 CD cord-268467-btfz6ye8 63 65 " " `` cord-268467-btfz6ye8 63 66 in in IN cord-268467-btfz6ye8 63 67 a a DT cord-268467-btfz6ye8 63 68 Beckman Beckman NNP cord-268467-btfz6ye8 63 69 SW28.1 sw28.1 JJ cord-268467-btfz6ye8 63 70 rotor rotor NN cord-268467-btfz6ye8 63 71 . . . cord-268467-btfz6ye8 64 1 The the DT cord-268467-btfz6ye8 64 2 virus virus NN cord-268467-btfz6ye8 64 3 band band NN cord-268467-btfz6ye8 64 4 at at IN cord-268467-btfz6ye8 64 5 the the DT cord-268467-btfz6ye8 64 6 interface interface NN cord-268467-btfz6ye8 64 7 between between IN cord-268467-btfz6ye8 64 8 50 50 CD cord-268467-btfz6ye8 64 9 and and CC cord-268467-btfz6ye8 64 10 30 30 CD cord-268467-btfz6ye8 64 11 % % NN cord-268467-btfz6ye8 64 12 sucrose sucrose NN cord-268467-btfz6ye8 64 13 was be VBD cord-268467-btfz6ye8 64 14 collected collect VBN cord-268467-btfz6ye8 64 15 and and CC cord-268467-btfz6ye8 64 16 diluted dilute VBN cord-268467-btfz6ye8 64 17 threefold threefold NN cord-268467-btfz6ye8 64 18 with with IN cord-268467-btfz6ye8 64 19 NTE NTE NNP cord-268467-btfz6ye8 64 20 buffer buffer NN cord-268467-btfz6ye8 64 21 . . . cord-268467-btfz6ye8 65 1 The the DT cord-268467-btfz6ye8 65 2 diluted diluted JJ cord-268467-btfz6ye8 65 3 virus virus NN cord-268467-btfz6ye8 65 4 suspension suspension NN cord-268467-btfz6ye8 65 5 was be VBD cord-268467-btfz6ye8 65 6 centrifuged centrifuge VBN cord-268467-btfz6ye8 65 7 on on IN cord-268467-btfz6ye8 65 8 a a DT cord-268467-btfz6ye8 65 9 linear linear JJ cord-268467-btfz6ye8 65 10 sucrose sucrose NN cord-268467-btfz6ye8 65 11 gradient gradient NN cord-268467-btfz6ye8 65 12 at at IN cord-268467-btfz6ye8 65 13 26,000 26,000 CD cord-268467-btfz6ye8 65 14 rpm rpm NN cord-268467-btfz6ye8 65 15 in in IN cord-268467-btfz6ye8 65 16 an an DT cord-268467-btfz6ye8 65 17 SW28.1 sw28.1 JJ cord-268467-btfz6ye8 65 18 rotor rotor NN cord-268467-btfz6ye8 65 19 for for IN cord-268467-btfz6ye8 65 20 4 4 CD cord-268467-btfz6ye8 65 21 hr hr NN cord-268467-btfz6ye8 65 22 at at IN cord-268467-btfz6ye8 65 23 4 4 CD cord-268467-btfz6ye8 65 24 " " '' cord-268467-btfz6ye8 65 25 . . . cord-268467-btfz6ye8 66 1 The the DT cord-268467-btfz6ye8 66 2 virus virus NN cord-268467-btfz6ye8 66 3 band band NN cord-268467-btfz6ye8 66 4 was be VBD cord-268467-btfz6ye8 66 5 collected collect VBN cord-268467-btfz6ye8 66 6 and and CC cord-268467-btfz6ye8 66 7 treated treat VBN cord-268467-btfz6ye8 66 8 with with IN cord-268467-btfz6ye8 66 9 proteinase proteinase NN cord-268467-btfz6ye8 66 10 K k NN cord-268467-btfz6ye8 66 11 ( ( -LRB- cord-268467-btfz6ye8 66 12 0.2 0.2 CD cord-268467-btfz6ye8 66 13 mg mg NNP cord-268467-btfz6ye8 66 14 / / SYM cord-268467-btfz6ye8 66 15 ml ml NNP cord-268467-btfz6ye8 66 16 ) ) -RRB- cord-268467-btfz6ye8 66 17 for for IN cord-268467-btfz6ye8 66 18 20 20 CD cord-268467-btfz6ye8 66 19 min min NN cord-268467-btfz6ye8 66 20 at at IN cord-268467-btfz6ye8 66 21 37 37 CD cord-268467-btfz6ye8 66 22 " " '' cord-268467-btfz6ye8 66 23 , , , cord-268467-btfz6ye8 66 24 followed follow VBN cord-268467-btfz6ye8 66 25 by by IN cord-268467-btfz6ye8 66 26 1 1 CD cord-268467-btfz6ye8 66 27 % % NN cord-268467-btfz6ye8 66 28 SDS SDS NNP cord-268467-btfz6ye8 66 29 for for IN cord-268467-btfz6ye8 66 30 30 30 CD cord-268467-btfz6ye8 66 31 min min NN cord-268467-btfz6ye8 66 32 at at IN cord-268467-btfz6ye8 66 33 37 37 CD cord-268467-btfz6ye8 66 34 " " '' cord-268467-btfz6ye8 66 35 . . . cord-268467-btfz6ye8 67 1 Genomic genomic JJ cord-268467-btfz6ye8 67 2 RNAwas rnawas XX cord-268467-btfz6ye8 67 3 extracted extract VBN cord-268467-btfz6ye8 67 4 with with IN cord-268467-btfz6ye8 67 5 phenol phenol NN cord-268467-btfz6ye8 67 6 and and CC cord-268467-btfz6ye8 67 7 then then RB cord-268467-btfz6ye8 67 8 with with IN cord-268467-btfz6ye8 67 9 phenol phenol NN cord-268467-btfz6ye8 67 10 / / SYM cord-268467-btfz6ye8 67 11 chloroform chloroform NN cord-268467-btfz6ye8 67 12 , , , cord-268467-btfz6ye8 67 13 and and CC cord-268467-btfz6ye8 67 14 precipitated precipitate VBN cord-268467-btfz6ye8 67 15 with with IN cord-268467-btfz6ye8 67 16 ethanol ethanol NN cord-268467-btfz6ye8 67 17 . . . cord-268467-btfz6ye8 68 1 Monolayers monolayer NNS cord-268467-btfz6ye8 68 2 of of IN cord-268467-btfz6ye8 68 3 L132 L132 NNP cord-268467-btfz6ye8 68 4 cells cell NNS cord-268467-btfz6ye8 68 5 grown grow VBN cord-268467-btfz6ye8 68 6 in in IN cord-268467-btfz6ye8 68 7 100 100 CD cord-268467-btfz6ye8 68 8 X x CD cord-268467-btfz6ye8 68 9 20-mm 20-mm CD cord-268467-btfz6ye8 68 10 culture culture NN cord-268467-btfz6ye8 68 11 dishes dish NNS cord-268467-btfz6ye8 69 1 were be VBD cord-268467-btfz6ye8 69 2 infected infect VBN cord-268467-btfz6ye8 69 3 with with IN cord-268467-btfz6ye8 69 4 HCV-229E. HCV-229E. NNP cord-268467-btfz6ye8 69 5 Cells cell NNS cord-268467-btfz6ye8 69 6 were be VBD cord-268467-btfz6ye8 69 7 incubated incubate VBN cord-268467-btfz6ye8 69 8 in in IN cord-268467-btfz6ye8 69 9 phosphate phosphate NN cord-268467-btfz6ye8 69 10 - - HYPH cord-268467-btfz6ye8 69 11 free free JJ cord-268467-btfz6ye8 69 12 DMEM DMEM NNP cord-268467-btfz6ye8 69 13 containing contain VBG cord-268467-btfz6ye8 69 14 1 1 CD cord-268467-btfz6ye8 69 15 % % NN cord-268467-btfz6ye8 69 16 dialyzed dialyzed JJ cord-268467-btfz6ye8 69 17 fetal fetal JJ cord-268467-btfz6ye8 69 18 calf calf NN cord-268467-btfz6ye8 69 19 serum serum NN cord-268467-btfz6ye8 69 20 4 4 CD cord-268467-btfz6ye8 69 21 hr hr NN cord-268467-btfz6ye8 69 22 prior prior RB cord-268467-btfz6ye8 69 23 to to IN cord-268467-btfz6ye8 69 24 RNA RNA NNP cord-268467-btfz6ye8 69 25 extraction extraction NN cord-268467-btfz6ye8 69 26 . . . cord-268467-btfz6ye8 70 1 Actinomycin Actinomycin NNP cord-268467-btfz6ye8 70 2 D D NNP cord-268467-btfz6ye8 70 3 ( ( -LRB- cord-268467-btfz6ye8 70 4 1 1 CD cord-268467-btfz6ye8 70 5 pg pg NN cord-268467-btfz6ye8 70 6 / / SYM cord-268467-btfz6ye8 70 7 ml ml NN cord-268467-btfz6ye8 70 8 ) ) -RRB- cord-268467-btfz6ye8 70 9 ( ( -LRB- cord-268467-btfz6ye8 70 10 Sigma Sigma NNP cord-268467-btfz6ye8 70 11 ) ) -RRB- cord-268467-btfz6ye8 70 12 and and CC cord-268467-btfz6ye8 70 13 [ [ -LRB- cord-268467-btfz6ye8 70 14 3zP 3zp CD cord-268467-btfz6ye8 70 15 ] ] -RRB- cord-268467-btfz6ye8 70 16 or or CC cord-268467-btfz6ye8 70 17 - - HYPH cord-268467-btfz6ye8 70 18 thophosphate thophosphate NN cord-268467-btfz6ye8 70 19 ( ( -LRB- cord-268467-btfz6ye8 70 20 70 70 CD cord-268467-btfz6ye8 70 21 & & CC cord-268467-btfz6ye8 70 22 i i NNP cord-268467-btfz6ye8 70 23 / / SYM cord-268467-btfz6ye8 70 24 ml ml NNP cord-268467-btfz6ye8 70 25 ) ) -RRB- cord-268467-btfz6ye8 70 26 ( ( -LRB- cord-268467-btfz6ye8 70 27 ICN ICN NNP cord-268467-btfz6ye8 70 28 Radiochemicals Radiochemicals NNP cord-268467-btfz6ye8 70 29 ) ) -RRB- cord-268467-btfz6ye8 70 30 were be VBD cord-268467-btfz6ye8 70 31 added add VBN cord-268467-btfz6ye8 70 32 at at IN cord-268467-btfz6ye8 70 33 3 3 CD cord-268467-btfz6ye8 70 34 and and CC cord-268467-btfz6ye8 70 35 2 2 CD cord-268467-btfz6ye8 70 36 hr hr NN cord-268467-btfz6ye8 70 37 , , , cord-268467-btfz6ye8 70 38 respectively respectively RB cord-268467-btfz6ye8 70 39 , , , cord-268467-btfz6ye8 70 40 prior prior RB cord-268467-btfz6ye8 70 41 to to IN cord-268467-btfz6ye8 70 42 RNA RNA NNP cord-268467-btfz6ye8 70 43 extraction extraction NN cord-268467-btfz6ye8 70 44 at at IN cord-268467-btfz6ye8 70 45 15 15 CD cord-268467-btfz6ye8 70 46 hr hr NN cord-268467-btfz6ye8 70 47 postinfection postinfection NN cord-268467-btfz6ye8 70 48 ( ( -LRB- cord-268467-btfz6ye8 70 49 p.i p.i NNP cord-268467-btfz6ye8 70 50 . . NNP cord-268467-btfz6ye8 70 51 ) ) -RRB- cord-268467-btfz6ye8 70 52 . . . cord-268467-btfz6ye8 71 1 Cells cell NNS cord-268467-btfz6ye8 71 2 were be VBD cord-268467-btfz6ye8 71 3 collected collect VBN cord-268467-btfz6ye8 71 4 in in IN cord-268467-btfz6ye8 71 5 cold cold JJ cord-268467-btfz6ye8 71 6 phosphatebuffered phosphatebuffered JJ cord-268467-btfz6ye8 71 7 saline saline NN cord-268467-btfz6ye8 71 8 and and CC cord-268467-btfz6ye8 71 9 centrifuged centrifuge VBD cord-268467-btfz6ye8 71 10 at at IN cord-268467-btfz6ye8 71 11 5000 5000 CD cord-268467-btfz6ye8 71 12 rpm rpm NN cord-268467-btfz6ye8 71 13 for for IN cord-268467-btfz6ye8 71 14 3 3 CD cord-268467-btfz6ye8 71 15 min min NN cord-268467-btfz6ye8 71 16 at at IN cord-268467-btfz6ye8 71 17 4 4 CD cord-268467-btfz6ye8 71 18 " " '' cord-268467-btfz6ye8 71 19 . . . cord-268467-btfz6ye8 72 1 The the DT cord-268467-btfz6ye8 72 2 pellet pellet NN cord-268467-btfz6ye8 72 3 was be VBD cord-268467-btfz6ye8 72 4 mixed mix VBN cord-268467-btfz6ye8 72 5 with with IN cord-268467-btfz6ye8 72 6 cold cold JJ cord-268467-btfz6ye8 72 7 0.5 0.5 CD cord-268467-btfz6ye8 72 8 % % NN cord-268467-btfz6ye8 72 9 Nonidet Nonidet NNP cord-268467-btfz6ye8 72 10 - - HYPH cord-268467-btfz6ye8 72 11 P40 P40 NNP cord-268467-btfz6ye8 72 12 in in IN cord-268467-btfz6ye8 72 13 NTE NTE NNP cord-268467-btfz6ye8 72 14 buffer buffer NN cord-268467-btfz6ye8 72 15 , , , cord-268467-btfz6ye8 72 16 incubated incubate VBN cord-268467-btfz6ye8 72 17 for for IN cord-268467-btfz6ye8 72 18 10 10 CD cord-268467-btfz6ye8 72 19 min min NN cord-268467-btfz6ye8 72 20 at at IN cord-268467-btfz6ye8 72 21 4 4 CD cord-268467-btfz6ye8 72 22 ' ' '' cord-268467-btfz6ye8 72 23 , , , cord-268467-btfz6ye8 72 24 and and CC cord-268467-btfz6ye8 72 25 then then RB cord-268467-btfz6ye8 72 26 centrifuged centrifuge VBN cord-268467-btfz6ye8 72 27 at at IN cord-268467-btfz6ye8 72 28 5000 5000 CD cord-268467-btfz6ye8 72 29 rpm rpm NN cord-268467-btfz6ye8 72 30 for for IN cord-268467-btfz6ye8 72 31 3 3 CD cord-268467-btfz6ye8 72 32 min min NN cord-268467-btfz6ye8 72 33 . . . cord-268467-btfz6ye8 73 1 The the DT cord-268467-btfz6ye8 73 2 supernatant supernatant NN cord-268467-btfz6ye8 73 3 was be VBD cord-268467-btfz6ye8 73 4 transferred transfer VBN cord-268467-btfz6ye8 73 5 to to IN cord-268467-btfz6ye8 73 6 a a DT cord-268467-btfz6ye8 73 7 fresh fresh JJ cord-268467-btfz6ye8 73 8 tube tube NN cord-268467-btfz6ye8 73 9 containing contain VBG cord-268467-btfz6ye8 73 10 l/10 l/10 NNP cord-268467-btfz6ye8 73 11 vol vol NNP cord-268467-btfz6ye8 73 12 of of IN cord-268467-btfz6ye8 73 13 10 10 CD cord-268467-btfz6ye8 73 14 % % NN cord-268467-btfz6ye8 73 15 SDS SDS NNP cord-268467-btfz6ye8 73 16 at at IN cord-268467-btfz6ye8 73 17 room room NN cord-268467-btfz6ye8 73 18 temperature temperature NN cord-268467-btfz6ye8 73 19 and and CC cord-268467-btfz6ye8 73 20 vortexed vortexe VBD cord-268467-btfz6ye8 73 21 briefly briefly RB cord-268467-btfz6ye8 73 22 . . . cord-268467-btfz6ye8 74 1 Intracellular Intracellular NNP cord-268467-btfz6ye8 74 2 RNA RNA NNP cord-268467-btfz6ye8 74 3 was be VBD cord-268467-btfz6ye8 74 4 extracted extract VBN cord-268467-btfz6ye8 74 5 with with IN cord-268467-btfz6ye8 74 6 phenol phenol NN cord-268467-btfz6ye8 74 7 and and CC cord-268467-btfz6ye8 74 8 phenol/ phenol/ VB cord-268467-btfz6ye8 74 9 chloroform chloroform NN cord-268467-btfz6ye8 74 10 and and CC cord-268467-btfz6ye8 74 11 precipitated precipitate VBN cord-268467-btfz6ye8 74 12 with with IN cord-268467-btfz6ye8 74 13 ethanol ethanol NN cord-268467-btfz6ye8 74 14 . . . cord-268467-btfz6ye8 75 1 Poly(A)-containing poly(a)-containe VBG cord-268467-btfz6ye8 75 2 RNA RNA NNP cord-268467-btfz6ye8 75 3 was be VBD cord-268467-btfz6ye8 75 4 selected select VBN cord-268467-btfz6ye8 75 5 by by IN cord-268467-btfz6ye8 75 6 oligo(dT)-cellulose oligo(dT)-cellulose NNP cord-268467-btfz6ye8 75 7 chromatography chromatography NN cord-268467-btfz6ye8 75 8 as as IN cord-268467-btfz6ye8 75 9 previously previously RB cord-268467-btfz6ye8 75 10 described describe VBN cord-268467-btfz6ye8 75 11 ( ( -LRB- cord-268467-btfz6ye8 75 12 Makino Makino NNP cord-268467-btfz6ye8 75 13 et et NNP cord-268467-btfz6ye8 75 14 al al NNP cord-268467-btfz6ye8 75 15 . . NNP cord-268467-btfz6ye8 75 16 , , , cord-268467-btfz6ye8 75 17 1984 1984 CD cord-268467-btfz6ye8 75 18 ) ) -RRB- cord-268467-btfz6ye8 75 19 . . . cord-268467-btfz6ye8 76 1 To to TO cord-268467-btfz6ye8 76 2 examine examine VB cord-268467-btfz6ye8 76 3 the the DT cord-268467-btfz6ye8 76 4 kinetics kinetic NNS cord-268467-btfz6ye8 76 5 of of IN cord-268467-btfz6ye8 76 6 viral viral JJ cord-268467-btfz6ye8 76 7 mRNA mRNA NNP cord-268467-btfz6ye8 76 8 synthesis synthesis NN cord-268467-btfz6ye8 76 9 , , , cord-268467-btfz6ye8 76 10 intracellular intracellular JJ cord-268467-btfz6ye8 76 11 RNA RNA NNP cord-268467-btfz6ye8 76 12 was be VBD cord-268467-btfz6ye8 76 13 extracted extract VBN cord-268467-btfz6ye8 76 14 from from IN cord-268467-btfz6ye8 76 15 virus virus NN cord-268467-btfz6ye8 76 16 - - HYPH cord-268467-btfz6ye8 76 17 infected infect VBN cord-268467-btfz6ye8 76 18 L132 L132 NNP cord-268467-btfz6ye8 76 19 monolayers monolayer NNS cord-268467-btfz6ye8 76 20 in in IN cord-268467-btfz6ye8 76 21 60 60 CD cord-268467-btfz6ye8 76 22 X x NN cord-268467-btfz6ye8 76 23 15-mm 15-mm CD cord-268467-btfz6ye8 76 24 culture culture NN cord-268467-btfz6ye8 76 25 dishes dish NNS cord-268467-btfz6ye8 76 26 at at IN cord-268467-btfz6ye8 76 27 7 7 CD cord-268467-btfz6ye8 76 28 , , , cord-268467-btfz6ye8 76 29 21 21 CD cord-268467-btfz6ye8 76 30 , , , cord-268467-btfz6ye8 76 31 29 29 CD cord-268467-btfz6ye8 76 32 , , , cord-268467-btfz6ye8 76 33 46 46 CD cord-268467-btfz6ye8 76 34 , , , cord-268467-btfz6ye8 76 35 and and CC cord-268467-btfz6ye8 76 36 58 58 CD cord-268467-btfz6ye8 76 37 hr hr NN cord-268467-btfz6ye8 76 38 postinfection postinfection NN cord-268467-btfz6ye8 76 39 . . . cord-268467-btfz6ye8 76 40 cDNA cDNA NNP cord-268467-btfz6ye8 76 41 cloning clone VBG cord-268467-btfz6ye8 76 42 cDNA cdna NN cord-268467-btfz6ye8 76 43 cloning cloning NN cord-268467-btfz6ye8 76 44 was be VBD cord-268467-btfz6ye8 76 45 performed perform VBN cord-268467-btfz6ye8 76 46 using use VBG cord-268467-btfz6ye8 76 47 a a DT cord-268467-btfz6ye8 76 48 modified modify VBN cord-268467-btfz6ye8 76 49 method method NN cord-268467-btfz6ye8 76 50 of of IN cord-268467-btfz6ye8 76 51 Gubler Gubler NNP cord-268467-btfz6ye8 76 52 and and CC cord-268467-btfz6ye8 76 53 Hoffman Hoffman NNP cord-268467-btfz6ye8 76 54 ( ( -LRB- cord-268467-btfz6ye8 76 55 1983 1983 CD cord-268467-btfz6ye8 76 56 ) ) -RRB- cord-268467-btfz6ye8 76 57 . . . cord-268467-btfz6ye8 77 1 The the DT cord-268467-btfz6ye8 77 2 poly(A)containing poly(A)containing NNP cord-268467-btfz6ye8 77 3 RNA RNA NNP cord-268467-btfz6ye8 77 4 extracted extract VBN cord-268467-btfz6ye8 77 5 from from IN cord-268467-btfz6ye8 77 6 229E 229e CD cord-268467-btfz6ye8 77 7 - - HYPH cord-268467-btfz6ye8 77 8 infected infect VBN cord-268467-btfz6ye8 77 9 L132 L132 NNP cord-268467-btfz6ye8 77 10 monolayers monolayer NNS cord-268467-btfz6ye8 77 11 was be VBD cord-268467-btfz6ye8 77 12 precipitated precipitate VBN cord-268467-btfz6ye8 77 13 , , , cord-268467-btfz6ye8 77 14 dried dry VBN cord-268467-btfz6ye8 77 15 , , , cord-268467-btfz6ye8 77 16 and and CC cord-268467-btfz6ye8 77 17 resuspended resuspend VBD cord-268467-btfz6ye8 77 18 in in IN cord-268467-btfz6ye8 77 19 6.72 6.72 CD cord-268467-btfz6ye8 77 20 ~1 ~1 CD cord-268467-btfz6ye8 77 21 of of IN cord-268467-btfz6ye8 77 22 autoclaved autoclave VBN cord-268467-btfz6ye8 77 23 water water NN cord-268467-btfz6ye8 77 24 . . . cord-268467-btfz6ye8 78 1 The the DT cord-268467-btfz6ye8 78 2 RNA RNA NNP cord-268467-btfz6ye8 78 3 was be VBD cord-268467-btfz6ye8 78 4 incubated incubate VBN cord-268467-btfz6ye8 78 5 with with IN cord-268467-btfz6ye8 78 6 10 10 CD cord-268467-btfz6ye8 78 7 mM mm CD cord-268467-btfz6ye8 78 8 methylmercuric methylmercuric JJ cord-268467-btfz6ye8 78 9 hydroxide hydroxide NN cord-268467-btfz6ye8 78 10 in in IN cord-268467-btfz6ye8 78 11 an an DT cord-268467-btfz6ye8 78 12 8 8 CD cord-268467-btfz6ye8 78 13 ~1 ~1 CD cord-268467-btfz6ye8 78 14 total total JJ cord-268467-btfz6ye8 78 15 volume volume NN cord-268467-btfz6ye8 78 16 for for IN cord-268467-btfz6ye8 78 17 10 10 CD cord-268467-btfz6ye8 78 18 min min NN cord-268467-btfz6ye8 78 19 at at IN cord-268467-btfz6ye8 78 20 room room NN cord-268467-btfz6ye8 78 21 temperature temperature NN cord-268467-btfz6ye8 78 22 . . . cord-268467-btfz6ye8 79 1 First first JJ cord-268467-btfz6ye8 79 2 - - HYPH cord-268467-btfz6ye8 79 3 strand strand NN cord-268467-btfz6ye8 79 4 cDNA cdna NN cord-268467-btfz6ye8 79 5 synthesis synthesis NN cord-268467-btfz6ye8 79 6 was be VBD cord-268467-btfz6ye8 79 7 carried carry VBN cord-268467-btfz6ye8 79 8 out out RP cord-268467-btfz6ye8 79 9 in in IN cord-268467-btfz6ye8 79 10 a a DT cord-268467-btfz6ye8 79 11 50-~1 50-~1 CD cord-268467-btfz6ye8 79 12 reaction reaction NN cord-268467-btfz6ye8 79 13 mixture mixture NN cord-268467-btfz6ye8 79 14 containing contain VBG cord-268467-btfz6ye8 79 15 60 60 CD cord-268467-btfz6ye8 79 16 units unit NNS cord-268467-btfz6ye8 80 1 RNasin RNasin NNP cord-268467-btfz6ye8 81 1 ( ( -LRB- cord-268467-btfz6ye8 81 2 Promega Promega NNP cord-268467-btfz6ye8 81 3 Biotec Biotec NNP cord-268467-btfz6ye8 81 4 ) ) -RRB- cord-268467-btfz6ye8 81 5 , , . cord-268467-btfz6ye8 82 1 10 10 CD cord-268467-btfz6ye8 82 2 mM mm CD cord-268467-btfz6ye8 82 3 MgCI MgCI NNP cord-268467-btfz6ye8 82 4 , , , cord-268467-btfz6ye8 82 5 , , , cord-268467-btfz6ye8 82 6 100 100 CD cord-268467-btfz6ye8 82 7 mM mm CD cord-268467-btfz6ye8 82 8 KCI KCI NNP cord-268467-btfz6ye8 82 9 , , , cord-268467-btfz6ye8 82 10 50 50 CD cord-268467-btfz6ye8 82 11 mM mM NNP cord-268467-btfz6ye8 82 12 Tris Tris NNP cord-268467-btfz6ye8 82 13 - - HYPH cord-268467-btfz6ye8 82 14 HCI HCI NNP cord-268467-btfz6ye8 82 15 ( ( -LRB- cord-268467-btfz6ye8 82 16 pH pH NNP cord-268467-btfz6ye8 82 17 8.3 8.3 CD cord-268467-btfz6ye8 82 18 at at IN cord-268467-btfz6ye8 82 19 42 42 CD cord-268467-btfz6ye8 82 20 " " '' cord-268467-btfz6ye8 82 21 ) ) -RRB- cord-268467-btfz6ye8 83 1 10 10 CD cord-268467-btfz6ye8 83 2 mM mm CD cord-268467-btfz6ye8 83 3 DTT DTT NNP cord-268467-btfz6ye8 83 4 , , , cord-268467-btfz6ye8 83 5 1.25 1.25 CD cord-268467-btfz6ye8 83 6 mM mm CD cord-268467-btfz6ye8 83 7 dNTPs dNTPs NNP cord-268467-btfz6ye8 83 8 , , , cord-268467-btfz6ye8 83 9 40 40 CD cord-268467-btfz6ye8 83 10 & & CC cord-268467-btfz6ye8 83 11 i i PRP cord-268467-btfz6ye8 83 12 [ [ -LRB- cord-268467-btfz6ye8 83 13 a-32P]dATP a-32P]dATP NNP cord-268467-btfz6ye8 83 14 ( ( -LRB- cord-268467-btfz6ye8 83 15 3000 3000 CD cord-268467-btfz6ye8 83 16 Ci ci NN cord-268467-btfz6ye8 83 17 / / SYM cord-268467-btfz6ye8 83 18 mmol mmol NN cord-268467-btfz6ye8 83 19 ) ) -RRB- cord-268467-btfz6ye8 83 20 , , , cord-268467-btfz6ye8 83 21 28 28 CD cord-268467-btfz6ye8 83 22 mM mM NNP cord-268467-btfz6ye8 83 23 , , , cord-268467-btfz6ye8 83 24 & & CC cord-268467-btfz6ye8 83 25 mercaptoethanol mercaptoethanol NNP cord-268467-btfz6ye8 83 26 , , , cord-268467-btfz6ye8 83 27 and and CC cord-268467-btfz6ye8 83 28 10 10 CD cord-268467-btfz6ye8 83 29 ng ng NNP cord-268467-btfz6ye8 83 30 oligo(dT),2-,s oligo(dt),2-,s NN cord-268467-btfz6ye8 83 31 primer primer NN cord-268467-btfz6ye8 83 32 . . . cord-268467-btfz6ye8 84 1 After after IN cord-268467-btfz6ye8 84 2 5 5 CD cord-268467-btfz6ye8 84 3 min min NN cord-268467-btfz6ye8 84 4 at at IN cord-268467-btfz6ye8 84 5 room room NN cord-268467-btfz6ye8 84 6 temperature temperature NN cord-268467-btfz6ye8 84 7 , , , cord-268467-btfz6ye8 84 8 40 40 CD cord-268467-btfz6ye8 84 9 units unit NNS cord-268467-btfz6ye8 84 10 of of IN cord-268467-btfz6ye8 84 11 AMV AMV NNP cord-268467-btfz6ye8 84 12 reverse reverse NN cord-268467-btfz6ye8 84 13 transcriptase transcriptase NN cord-268467-btfz6ye8 84 14 ( ( -LRB- cord-268467-btfz6ye8 84 15 Life Life NNP cord-268467-btfz6ye8 84 16 Science Science NNP cord-268467-btfz6ye8 84 17 ) ) -RRB- cord-268467-btfz6ye8 84 18 was be VBD cord-268467-btfz6ye8 84 19 added add VBN cord-268467-btfz6ye8 84 20 and and CC cord-268467-btfz6ye8 84 21 the the DT cord-268467-btfz6ye8 84 22 mixture mixture NN cord-268467-btfz6ye8 84 23 was be VBD cord-268467-btfz6ye8 84 24 incubated incubate VBN cord-268467-btfz6ye8 84 25 for for IN cord-268467-btfz6ye8 84 26 1 1 CD cord-268467-btfz6ye8 84 27 hr hr NN cord-268467-btfz6ye8 84 28 at at IN cord-268467-btfz6ye8 84 29 42 42 CD cord-268467-btfz6ye8 84 30 " " '' cord-268467-btfz6ye8 84 31 . . . cord-268467-btfz6ye8 85 1 The the DT cord-268467-btfz6ye8 85 2 reaction reaction NN cord-268467-btfz6ye8 85 3 was be VBD cord-268467-btfz6ye8 85 4 stopped stop VBN cord-268467-btfz6ye8 85 5 by by IN cord-268467-btfz6ye8 85 6 adding add VBG cord-268467-btfz6ye8 85 7 4.4 4.4 CD cord-268467-btfz6ye8 85 8 ~1 ~1 CD cord-268467-btfz6ye8 85 9 of of IN cord-268467-btfz6ye8 85 10 250 250 CD cord-268467-btfz6ye8 85 11 mM mm CD cord-268467-btfz6ye8 85 12 EDTA edta NN cord-268467-btfz6ye8 85 13 . . . cord-268467-btfz6ye8 86 1 The the DT cord-268467-btfz6ye8 86 2 products product NNS cord-268467-btfz6ye8 86 3 were be VBD cord-268467-btfz6ye8 86 4 extracted extract VBN cord-268467-btfz6ye8 86 5 with with IN cord-268467-btfz6ye8 86 6 phenol/ phenol/ JJ cord-268467-btfz6ye8 86 7 chloroform chloroform NN cord-268467-btfz6ye8 86 8 and and CC cord-268467-btfz6ye8 86 9 precipitated precipitate VBN cord-268467-btfz6ye8 86 10 with with IN cord-268467-btfz6ye8 86 11 ethanol ethanol NN cord-268467-btfz6ye8 86 12 containing contain VBG cord-268467-btfz6ye8 86 13 0.3 0.3 CD cord-268467-btfz6ye8 86 14 M m CD cord-268467-btfz6ye8 86 15 ammonium ammonium NN cord-268467-btfz6ye8 86 16 acetate acetate NN cord-268467-btfz6ye8 86 17 . . . cord-268467-btfz6ye8 87 1 For for IN cord-268467-btfz6ye8 87 2 second second JJ cord-268467-btfz6ye8 87 3 - - HYPH cord-268467-btfz6ye8 87 4 strand strand NN cord-268467-btfz6ye8 87 5 synthesis synthesis NN cord-268467-btfz6ye8 87 6 , , , cord-268467-btfz6ye8 87 7 the the DT cord-268467-btfz6ye8 87 8 lOO-~1 lOO-~1 NNP cord-268467-btfz6ye8 87 9 reaction reaction NN cord-268467-btfz6ye8 87 10 mixture mixture NN cord-268467-btfz6ye8 87 11 contained contain VBD cord-268467-btfz6ye8 87 12 5 5 CD cord-268467-btfz6ye8 87 13 mM mm CD cord-268467-btfz6ye8 87 14 MgCI MgCI NNP cord-268467-btfz6ye8 87 15 , , , cord-268467-btfz6ye8 87 16 , , , cord-268467-btfz6ye8 87 17 100 100 CD cord-268467-btfz6ye8 87 18 mM mm CD cord-268467-btfz6ye8 87 19 KCI KCI NNP cord-268467-btfz6ye8 87 20 , , , cord-268467-btfz6ye8 87 21 20 20 CD cord-268467-btfz6ye8 87 22 mM mM NNP cord-268467-btfz6ye8 87 23 Tris Tris NNP cord-268467-btfz6ye8 87 24 - - HYPH cord-268467-btfz6ye8 87 25 HCI HCI NNP cord-268467-btfz6ye8 87 26 ( ( -LRB- cord-268467-btfz6ye8 87 27 pH pH NNP cord-268467-btfz6ye8 87 28 7.5 7.5 CD cord-268467-btfz6ye8 87 29 ) ) -RRB- cord-268467-btfz6ye8 87 30 50 50 CD cord-268467-btfz6ye8 88 1 pgl pgl NNP cord-268467-btfz6ye8 88 2 ml ml NNP cord-268467-btfz6ye8 88 3 bovine bovine JJ cord-268467-btfz6ye8 88 4 serum serum NN cord-268467-btfz6ye8 88 5 albumin albumin NN cord-268467-btfz6ye8 88 6 ( ( -LRB- cord-268467-btfz6ye8 88 7 BSA BSA NNP cord-268467-btfz6ye8 88 8 ) ) -RRB- cord-268467-btfz6ye8 88 9 , , , cord-268467-btfz6ye8 88 10 10 10 CD cord-268467-btfz6ye8 88 11 mM mm CD cord-268467-btfz6ye8 88 12 ammonium ammonium NN cord-268467-btfz6ye8 88 13 sulfate sulfate NN cord-268467-btfz6ye8 88 14 , , , cord-268467-btfz6ye8 88 15 0.15 0.15 CD cord-268467-btfz6ye8 88 16 mM mm CD cord-268467-btfz6ye8 88 17 P P NNP cord-268467-btfz6ye8 88 18 - - HYPH cord-268467-btfz6ye8 88 19 NAD NAD NNP cord-268467-btfz6ye8 88 20 , , , cord-268467-btfz6ye8 88 21 100 100 CD cord-268467-btfz6ye8 88 22 pM pm NN cord-268467-btfz6ye8 89 1 dNTPs dntp NNS cord-268467-btfz6ye8 89 2 , , , cord-268467-btfz6ye8 89 3 25 25 CD cord-268467-btfz6ye8 89 4 units unit NNS cord-268467-btfz6ye8 89 5 of of IN cord-268467-btfz6ye8 89 6 Escherichia Escherichia NNP cord-268467-btfz6ye8 89 7 co co NN cord-268467-btfz6ye8 89 8 / / SYM cord-268467-btfz6ye8 89 9 i i PRP cord-268467-btfz6ye8 90 1 DNA dna NN cord-268467-btfz6ye8 90 2 polymerase polymerase NN cord-268467-btfz6ye8 90 3 I i NN cord-268467-btfz6ye8 90 4 , , , cord-268467-btfz6ye8 90 5 2 2 CD cord-268467-btfz6ye8 90 6 units unit NNS cord-268467-btfz6ye8 90 7 of of IN cord-268467-btfz6ye8 90 8 E. E. NNP cord-268467-btfz6ye8 90 9 co co NNP cord-268467-btfz6ye8 90 10 / / SYM cord-268467-btfz6ye8 90 11 i i PRP cord-268467-btfz6ye8 90 12 DNA DNA NNP cord-268467-btfz6ye8 90 13 ligase ligase NN cord-268467-btfz6ye8 90 14 , , , cord-268467-btfz6ye8 90 15 and and CC cord-268467-btfz6ye8 90 16 0.8 0.8 CD cord-268467-btfz6ye8 90 17 units unit NNS cord-268467-btfz6ye8 90 18 of of IN cord-268467-btfz6ye8 90 19 RNase RNase NNP cord-268467-btfz6ye8 90 20 H. H. NNP cord-268467-btfz6ye8 90 21 Sequential sequential JJ cord-268467-btfz6ye8 90 22 incubations incubation NNS cord-268467-btfz6ye8 90 23 were be VBD cord-268467-btfz6ye8 90 24 for for IN cord-268467-btfz6ye8 90 25 1 1 CD cord-268467-btfz6ye8 90 26 hr hr NN cord-268467-btfz6ye8 90 27 at at IN cord-268467-btfz6ye8 90 28 12 12 CD cord-268467-btfz6ye8 90 29 " " '' cord-268467-btfz6ye8 90 30 and and CC cord-268467-btfz6ye8 90 31 1 1 CD cord-268467-btfz6ye8 90 32 hr hr NN cord-268467-btfz6ye8 90 33 at at IN cord-268467-btfz6ye8 90 34 22 22 CD cord-268467-btfz6ye8 90 35 " " '' cord-268467-btfz6ye8 90 36 . . . cord-268467-btfz6ye8 91 1 The the DT cord-268467-btfz6ye8 91 2 reaction reaction NN cord-268467-btfz6ye8 91 3 was be VBD cord-268467-btfz6ye8 91 4 stopped stop VBN cord-268467-btfz6ye8 91 5 by by IN cord-268467-btfz6ye8 91 6 the the DT cord-268467-btfz6ye8 91 7 addition addition NN cord-268467-btfz6ye8 91 8 of of IN cord-268467-btfz6ye8 91 9 8.7 8.7 CD cord-268467-btfz6ye8 91 10 ~1 ~1 CD cord-268467-btfz6ye8 91 11 of of IN cord-268467-btfz6ye8 91 12 250 250 CD cord-268467-btfz6ye8 91 13 mM mm CD cord-268467-btfz6ye8 91 14 EDTA EDTA NNP cord-268467-btfz6ye8 91 15 and and CC cord-268467-btfz6ye8 91 16 the the DT cord-268467-btfz6ye8 91 17 products product NNS cord-268467-btfz6ye8 91 18 were be VBD cord-268467-btfz6ye8 91 19 extracted extract VBN cord-268467-btfz6ye8 91 20 with with IN cord-268467-btfz6ye8 91 21 phenol/ phenol/ JJ cord-268467-btfz6ye8 91 22 chloroform chloroform NN cord-268467-btfz6ye8 91 23 and and CC cord-268467-btfz6ye8 91 24 precipitated precipitate VBN cord-268467-btfz6ye8 91 25 with with IN cord-268467-btfz6ye8 91 26 ethanol ethanol NN cord-268467-btfz6ye8 91 27 in in IN cord-268467-btfz6ye8 91 28 the the DT cord-268467-btfz6ye8 91 29 presence presence NN cord-268467-btfz6ye8 91 30 of of IN cord-268467-btfz6ye8 91 31 0.3 0.3 CD cord-268467-btfz6ye8 91 32 Mammonium Mammonium NNP cord-268467-btfz6ye8 91 33 acetate acetate NN cord-268467-btfz6ye8 91 34 . . . cord-268467-btfz6ye8 92 1 Homopolymeric Homopolymeric NNP cord-268467-btfz6ye8 92 2 tailing tailing NN cord-268467-btfz6ye8 92 3 of of IN cord-268467-btfz6ye8 92 4 double double RB cord-268467-btfz6ye8 92 5 - - HYPH cord-268467-btfz6ye8 92 6 stranded strand VBN cord-268467-btfz6ye8 92 7 cDNA cdna NN cord-268467-btfz6ye8 92 8 with with IN cord-268467-btfz6ye8 92 9 poly(C poly(C NNP cord-268467-btfz6ye8 92 10 ) ) -RRB- cord-268467-btfz6ye8 92 11 was be VBD cord-268467-btfz6ye8 92 12 carried carry VBN cord-268467-btfz6ye8 92 13 out out RP cord-268467-btfz6ye8 92 14 in in IN cord-268467-btfz6ye8 92 15 a a DT cord-268467-btfz6ye8 92 16 1 1 CD cord-268467-btfz6ye8 92 17 ~-PI ~-pi NN cord-268467-btfz6ye8 92 18 reaction reaction NN cord-268467-btfz6ye8 92 19 mixture mixture NN cord-268467-btfz6ye8 92 20 containing contain VBG cord-268467-btfz6ye8 92 21 10 10 CD cord-268467-btfz6ye8 92 22 units unit NNS cord-268467-btfz6ye8 92 23 of of IN cord-268467-btfz6ye8 92 24 terminal terminal JJ cord-268467-btfz6ye8 92 25 transferase transferase NN cord-268467-btfz6ye8 92 26 , , , cord-268467-btfz6ye8 92 27 200 200 CD cord-268467-btfz6ye8 92 28 mM mm CD cord-268467-btfz6ye8 92 29 potassium potassium NN cord-268467-btfz6ye8 92 30 cacodylate cacodylate NN cord-268467-btfz6ye8 92 31 , , , cord-268467-btfz6ye8 92 32 0.5 0.5 CD cord-268467-btfz6ye8 92 33 mM mm CD cord-268467-btfz6ye8 92 34 Co Co NNP cord-268467-btfz6ye8 92 35 & & CC cord-268467-btfz6ye8 92 36 , , , cord-268467-btfz6ye8 92 37 25 25 CD cord-268467-btfz6ye8 92 38 mM mM NNP cord-268467-btfz6ye8 92 39 Tris Tris NNP cord-268467-btfz6ye8 92 40 - - HYPH cord-268467-btfz6ye8 92 41 HCI HCI NNP cord-268467-btfz6ye8 92 42 ( ( -LRB- cord-268467-btfz6ye8 92 43 pH pH NNP cord-268467-btfz6ye8 92 44 6.9 6.9 CD cord-268467-btfz6ye8 92 45 ) ) -RRB- cord-268467-btfz6ye8 92 46 , , , cord-268467-btfz6ye8 92 47 2 2 CD cord-268467-btfz6ye8 92 48 rnn rnn NNP cord-268467-btfz6ye8 92 49 / / SYM cord-268467-btfz6ye8 92 50 l l NNP cord-268467-btfz6ye8 92 51 DlT DlT NNP cord-268467-btfz6ye8 92 52 , , , cord-268467-btfz6ye8 92 53 250 250 CD cord-268467-btfz6ye8 92 54 pg pg NN cord-268467-btfz6ye8 92 55 / / SYM cord-268467-btfz6ye8 92 56 ml ml NNP cord-268467-btfz6ye8 92 57 BSA BSA NNP cord-268467-btfz6ye8 92 58 , , , cord-268467-btfz6ye8 92 59 and and CC cord-268467-btfz6ye8 92 60 50 50 CD cord-268467-btfz6ye8 92 61 PM pm NN cord-268467-btfz6ye8 92 62 dCTP dCTP VBG cord-268467-btfz6ye8 92 63 at at IN cord-268467-btfz6ye8 92 64 37 37 CD cord-268467-btfz6ye8 92 65 " " `` cord-268467-btfz6ye8 92 66 for for IN cord-268467-btfz6ye8 92 67 4 4 CD cord-268467-btfz6ye8 92 68 min min NN cord-268467-btfz6ye8 92 69 . . . cord-268467-btfz6ye8 93 1 The the DT cord-268467-btfz6ye8 93 2 dC dc CD cord-268467-btfz6ye8 93 3 - - HYPH cord-268467-btfz6ye8 93 4 tailed tailed JJ cord-268467-btfz6ye8 93 5 double double RB cord-268467-btfz6ye8 93 6 - - HYPH cord-268467-btfz6ye8 93 7 stranded strand VBN cord-268467-btfz6ye8 93 8 DNA dna NN cord-268467-btfz6ye8 93 9 was be VBD cord-268467-btfz6ye8 93 10 annealed anneal VBN cord-268467-btfz6ye8 93 11 to to IN cord-268467-btfz6ye8 93 12 200 200 CD cord-268467-btfz6ye8 93 13 pg pg CD cord-268467-btfz6ye8 93 14 of of IN cord-268467-btfz6ye8 93 15 dG dG NNP cord-268467-btfz6ye8 93 16 - - HYPH cord-268467-btfz6ye8 93 17 tailed tailed JJ cord-268467-btfz6ye8 93 18 Pstl Pstl NNP cord-268467-btfz6ye8 93 19 - - HYPH cord-268467-btfz6ye8 93 20 cut cut VBN cord-268467-btfz6ye8 93 21 pBR322 pbr322 CD cord-268467-btfz6ye8 93 22 plasmid plasmid NN cord-268467-btfz6ye8 93 23 in in IN cord-268467-btfz6ye8 93 24 20 20 CD cord-268467-btfz6ye8 93 25 ~1 ~1 CD cord-268467-btfz6ye8 93 26 of of IN cord-268467-btfz6ye8 93 27 a a DT cord-268467-btfz6ye8 93 28 buffer buffer NN cord-268467-btfz6ye8 93 29 containing contain VBG cord-268467-btfz6ye8 93 30 10 10 CD cord-268467-btfz6ye8 93 31 mNI mNI NNP cord-268467-btfz6ye8 93 32 Tris Tris NNP cord-268467-btfz6ye8 93 33 - - HYPH cord-268467-btfz6ye8 93 34 HCI HCI NNP cord-268467-btfz6ye8 93 35 ( ( -LRB- cord-268467-btfz6ye8 93 36 pH pH NNP cord-268467-btfz6ye8 93 37 7.4 7.4 CD cord-268467-btfz6ye8 93 38 ) ) -RRB- cord-268467-btfz6ye8 93 39 , , , cord-268467-btfz6ye8 93 40 100 100 CD cord-268467-btfz6ye8 93 41 mM mm CD cord-268467-btfz6ye8 93 42 NaCI naci NN cord-268467-btfz6ye8 93 43 , , , cord-268467-btfz6ye8 93 44 and and CC cord-268467-btfz6ye8 93 45 0.25 0.25 CD cord-268467-btfz6ye8 93 46 mh mh NNP cord-268467-btfz6ye8 93 47 / / SYM cord-268467-btfz6ye8 93 48 l l NNP cord-268467-btfz6ye8 93 49 EDTA EDTA NNP cord-268467-btfz6ye8 93 50 . . . cord-268467-btfz6ye8 94 1 The the DT cord-268467-btfz6ye8 94 2 mixture mixture NN cord-268467-btfz6ye8 94 3 was be VBD cord-268467-btfz6ye8 94 4 incubated incubate VBN cord-268467-btfz6ye8 94 5 for for IN cord-268467-btfz6ye8 94 6 5 5 CD cord-268467-btfz6ye8 94 7 min min NN cord-268467-btfz6ye8 94 8 at at IN cord-268467-btfz6ye8 94 9 68 68 CD cord-268467-btfz6ye8 94 10 " " '' cord-268467-btfz6ye8 94 11 and and CC cord-268467-btfz6ye8 94 12 then then RB cord-268467-btfz6ye8 94 13 cooled cool VBD cord-268467-btfz6ye8 94 14 slowly slowly RB cord-268467-btfz6ye8 94 15 overnight overnight RB cord-268467-btfz6ye8 94 16 . . . cord-268467-btfz6ye8 95 1 The the DT cord-268467-btfz6ye8 95 2 annealed anneal VBN cord-268467-btfz6ye8 95 3 molecules molecule NNS cord-268467-btfz6ye8 95 4 were be VBD cord-268467-btfz6ye8 95 5 used use VBN cord-268467-btfz6ye8 95 6 to to TO cord-268467-btfz6ye8 95 7 transform transform VB cord-268467-btfz6ye8 95 8 E. E. NNP cord-268467-btfz6ye8 95 9 co/ co/ NNP cord-268467-btfz6ye8 96 1 i i PRP cord-268467-btfz6ye8 96 2 MCI061 mci061 VBP cord-268467-btfz6ye8 96 3 as as IN cord-268467-btfz6ye8 96 4 described describe VBN cord-268467-btfz6ye8 96 5 ( ( -LRB- cord-268467-btfz6ye8 96 6 Dagert Dagert NNP cord-268467-btfz6ye8 96 7 and and CC cord-268467-btfz6ye8 96 8 Erhlich Erhlich NNP cord-268467-btfz6ye8 96 9 , , , cord-268467-btfz6ye8 96 10 1979 1979 CD cord-268467-btfz6ye8 96 11 ) ) -RRB- cord-268467-btfz6ye8 96 12 . . . cord-268467-btfz6ye8 97 1 Colonies colony NNS cord-268467-btfz6ye8 97 2 grown grow VBN cord-268467-btfz6ye8 97 3 on on IN cord-268467-btfz6ye8 97 4 LB LB NNP cord-268467-btfz6ye8 97 5 / / SYM cord-268467-btfz6ye8 97 6 tetracycline tetracycline NN cord-268467-btfz6ye8 97 7 plates plate NNS cord-268467-btfz6ye8 97 8 were be VBD cord-268467-btfz6ye8 97 9 incubated incubate VBN cord-268467-btfz6ye8 97 10 at at IN cord-268467-btfz6ye8 97 11 37 37 CD cord-268467-btfz6ye8 97 12 " " `` cord-268467-btfz6ye8 97 13 for for IN cord-268467-btfz6ye8 97 14 12 12 CD cord-268467-btfz6ye8 97 15 hr hr NN cord-268467-btfz6ye8 97 16 and and CC cord-268467-btfz6ye8 97 17 transferred transfer VBN cord-268467-btfz6ye8 97 18 to to IN cord-268467-btfz6ye8 97 19 Colony Colony NNP cord-268467-btfz6ye8 97 20 / / SYM cord-268467-btfz6ye8 97 21 Plaque Plaque NNP cord-268467-btfz6ye8 97 22 Screen screen NN cord-268467-btfz6ye8 97 23 disks disk NNS cord-268467-btfz6ye8 97 24 ( ( -LRB- cord-268467-btfz6ye8 97 25 New New NNP cord-268467-btfz6ye8 97 26 England England NNP cord-268467-btfz6ye8 97 27 Nuclear Nuclear NNP cord-268467-btfz6ye8 97 28 ) ) -RRB- cord-268467-btfz6ye8 97 29 . . . cord-268467-btfz6ye8 98 1 Bacterial bacterial JJ cord-268467-btfz6ye8 98 2 lysis lysis NN cord-268467-btfz6ye8 98 3 and and CC cord-268467-btfz6ye8 98 4 DNA dna NN cord-268467-btfz6ye8 98 5 fixation fixation NN cord-268467-btfz6ye8 98 6 were be VBD cord-268467-btfz6ye8 98 7 carried carry VBN cord-268467-btfz6ye8 98 8 out out RP cord-268467-btfz6ye8 98 9 according accord VBG cord-268467-btfz6ye8 98 10 to to IN cord-268467-btfz6ye8 98 11 the the DT cord-268467-btfz6ye8 98 12 methods method NNS cord-268467-btfz6ye8 98 13 previously previously RB cord-268467-btfz6ye8 98 14 described describe VBN cord-268467-btfz6ye8 98 15 ( ( -LRB- cord-268467-btfz6ye8 98 16 Grunstein Grunstein NNP cord-268467-btfz6ye8 98 17 and and CC cord-268467-btfz6ye8 98 18 Hogness Hogness NNP cord-268467-btfz6ye8 98 19 , , , cord-268467-btfz6ye8 98 20 1975 1975 CD cord-268467-btfz6ye8 98 21 ) ) -RRB- cord-268467-btfz6ye8 98 22 . . . cord-268467-btfz6ye8 99 1 The the DT cord-268467-btfz6ye8 99 2 disks disk NNS cord-268467-btfz6ye8 99 3 were be VBD cord-268467-btfz6ye8 99 4 prehybridized prehybridize VBN cord-268467-btfz6ye8 99 5 in in IN cord-268467-btfz6ye8 99 6 a a DT cord-268467-btfz6ye8 99 7 solution solution NN cord-268467-btfz6ye8 99 8 containing contain VBG cord-268467-btfz6ye8 99 9 0.2 0.2 CD cord-268467-btfz6ye8 99 10 % % NN cord-268467-btfz6ye8 99 11 polyvinylpyrrolidone polyvinylpyrrolidone NN cord-268467-btfz6ye8 99 12 ( ( -LRB- cord-268467-btfz6ye8 99 13 MW MW NNP cord-268467-btfz6ye8 99 14 40,000 40,000 CD cord-268467-btfz6ye8 99 15 ) ) -RRB- cord-268467-btfz6ye8 99 16 , , , cord-268467-btfz6ye8 99 17 0.2 0.2 CD cord-268467-btfz6ye8 99 18 % % NN cord-268467-btfz6ye8 99 19 Ficoll Ficoll NNP cord-268467-btfz6ye8 99 20 ( ( -LRB- cord-268467-btfz6ye8 99 21 MW MW NNP cord-268467-btfz6ye8 99 22 400,000 400,000 CD cord-268467-btfz6ye8 99 23 ) ) -RRB- cord-268467-btfz6ye8 99 24 , , , cord-268467-btfz6ye8 99 25 0.2 0.2 CD cord-268467-btfz6ye8 99 26 % % NN cord-268467-btfz6ye8 99 27 BSA BSA NNP cord-268467-btfz6ye8 99 28 , , , cord-268467-btfz6ye8 99 29 0.05 0.05 CD cord-268467-btfz6ye8 99 30 MTris MTris NNP cord-268467-btfz6ye8 99 31 - - HYPH cord-268467-btfz6ye8 99 32 HCI HCI NNP cord-268467-btfz6ye8 99 33 ( ( -LRB- cord-268467-btfz6ye8 99 34 pH pH NNP cord-268467-btfz6ye8 99 35 7.5 7.5 CD cord-268467-btfz6ye8 99 36 ) ) -RRB- cord-268467-btfz6ye8 99 37 1 1 CD cord-268467-btfz6ye8 99 38 % % NN cord-268467-btfz6ye8 99 39 SDS SDS NNP cord-268467-btfz6ye8 99 40 , , , cord-268467-btfz6ye8 99 41 1 1 CD cord-268467-btfz6ye8 99 42 l\/I l\/i NN cord-268467-btfz6ye8 99 43 NaCI naci NN cord-268467-btfz6ye8 99 44 , , , cord-268467-btfz6ye8 99 45 10 10 CD cord-268467-btfz6ye8 99 46 % % NN cord-268467-btfz6ye8 99 47 dextran dextran NN cord-268467-btfz6ye8 99 48 sulfate sulfate NN cord-268467-btfz6ye8 99 49 , , , cord-268467-btfz6ye8 99 50 and and CC cord-268467-btfz6ye8 99 51 100 100 CD cord-268467-btfz6ye8 99 52 pg pg NN cord-268467-btfz6ye8 99 53 / / SYM cord-268467-btfz6ye8 99 54 ml ml IN cord-268467-btfz6ye8 99 55 denatured denatured JJ cord-268467-btfz6ye8 99 56 salmon salmon NN cord-268467-btfz6ye8 99 57 sperm sperm NN cord-268467-btfz6ye8 99 58 DNA dna NN cord-268467-btfz6ye8 99 59 at at IN cord-268467-btfz6ye8 99 60 65 65 CD cord-268467-btfz6ye8 99 61 " " '' cord-268467-btfz6ye8 99 62 for for IN cord-268467-btfz6ye8 99 63 6hr 6hr NN cord-268467-btfz6ye8 99 64 . . . cord-268467-btfz6ye8 100 1 Fragments fragment NNS cord-268467-btfz6ye8 100 2 derived derive VBN cord-268467-btfz6ye8 100 3 from from IN cord-268467-btfz6ye8 100 4 either either CC cord-268467-btfz6ye8 100 5 the the DT cord-268467-btfz6ye8 100 6 5'-or 5'-or CD cord-268467-btfz6ye8 100 7 3'-ends 3'-ends CD cord-268467-btfz6ye8 100 8 of of IN cord-268467-btfz6ye8 100 9 gene gene NN cord-268467-btfz6ye8 100 10 7 7 CD cord-268467-btfz6ye8 100 11 were be VBD cord-268467-btfz6ye8 100 12 labeled label VBN cord-268467-btfz6ye8 100 13 with with IN cord-268467-btfz6ye8 100 14 32P 32p CD cord-268467-btfz6ye8 100 15 by by IN cord-268467-btfz6ye8 100 16 nick nick NNP cord-268467-btfz6ye8 100 17 - - HYPH cord-268467-btfz6ye8 100 18 translation translation NNP cord-268467-btfz6ye8 100 19 and and CC cord-268467-btfz6ye8 100 20 added add VBD cord-268467-btfz6ye8 100 21 to to IN cord-268467-btfz6ye8 100 22 the the DT cord-268467-btfz6ye8 100 23 solution solution NN cord-268467-btfz6ye8 100 24 . . . cord-268467-btfz6ye8 101 1 Hybridization hybridization NN cord-268467-btfz6ye8 101 2 was be VBD cord-268467-btfz6ye8 101 3 carried carry VBN cord-268467-btfz6ye8 101 4 out out RP cord-268467-btfz6ye8 101 5 for for IN cord-268467-btfz6ye8 101 6 20 20 CD cord-268467-btfz6ye8 101 7 hr hr NN cord-268467-btfz6ye8 101 8 at at IN cord-268467-btfz6ye8 101 9 65 65 CD cord-268467-btfz6ye8 101 10 " " '' cord-268467-btfz6ye8 101 11 . . . cord-268467-btfz6ye8 102 1 The the DT cord-268467-btfz6ye8 102 2 disks disk NNS cord-268467-btfz6ye8 102 3 were be VBD cord-268467-btfz6ye8 102 4 then then RB cord-268467-btfz6ye8 102 5 washed wash VBN cord-268467-btfz6ye8 102 6 twice twice RB cord-268467-btfz6ye8 102 7 in in IN cord-268467-btfz6ye8 102 8 2~ 2~ CD cord-268467-btfz6ye8 102 9 SSC SSC NNP cord-268467-btfz6ye8 102 10 ( ( -LRB- cord-268467-btfz6ye8 102 11 0.3 0.3 CD cord-268467-btfz6ye8 102 12 lvI lvi JJ cord-268467-btfz6ye8 102 13 NaCI NaCI NNP cord-268467-btfz6ye8 102 14 , , , cord-268467-btfz6ye8 102 15 30 30 CD cord-268467-btfz6ye8 102 16 mlVI mlVI NNP cord-268467-btfz6ye8 102 17 sodium sodium NN cord-268467-btfz6ye8 102 18 citrate citrate NN cord-268467-btfz6ye8 102 19 ) ) -RRB- cord-268467-btfz6ye8 102 20 at at IN cord-268467-btfz6ye8 102 21 room room NN cord-268467-btfz6ye8 102 22 temperature temperature NN cord-268467-btfz6ye8 102 23 , , , cord-268467-btfz6ye8 102 24 twice twice RB cord-268467-btfz6ye8 102 25 in in IN cord-268467-btfz6ye8 102 26 2X 2x CD cord-268467-btfz6ye8 102 27 SSC SSC NNP cord-268467-btfz6ye8 102 28 containing contain VBG cord-268467-btfz6ye8 102 29 1 1 CD cord-268467-btfz6ye8 102 30 % % NN cord-268467-btfz6ye8 102 31 SDS SDS NNP cord-268467-btfz6ye8 102 32 for for IN cord-268467-btfz6ye8 102 33 30 30 CD cord-268467-btfz6ye8 102 34 min min NN cord-268467-btfz6ye8 102 35 at at IN cord-268467-btfz6ye8 102 36 65 65 CD cord-268467-btfz6ye8 102 37 " " '' cord-268467-btfz6ye8 102 38 , , , cord-268467-btfz6ye8 102 39 and and CC cord-268467-btfz6ye8 102 40 twice twice RB cord-268467-btfz6ye8 102 41 in in IN cord-268467-btfz6ye8 102 42 0.1 0.1 CD cord-268467-btfz6ye8 102 43 X x NN cord-268467-btfz6ye8 102 44 SSC SSC NNP cord-268467-btfz6ye8 102 45 at at IN cord-268467-btfz6ye8 102 46 room room NN cord-268467-btfz6ye8 102 47 temperature temperature NN cord-268467-btfz6ye8 102 48 for for IN cord-268467-btfz6ye8 102 49 30 30 CD cord-268467-btfz6ye8 102 50 min min NN cord-268467-btfz6ye8 102 51 . . . cord-268467-btfz6ye8 103 1 The the DT cord-268467-btfz6ye8 103 2 disks disk NNS cord-268467-btfz6ye8 103 3 were be VBD cord-268467-btfz6ye8 103 4 air air NN cord-268467-btfz6ye8 103 5 - - HYPH cord-268467-btfz6ye8 103 6 dried dry VBN cord-268467-btfz6ye8 103 7 and and CC cord-268467-btfz6ye8 103 8 exposed expose VBN cord-268467-btfz6ye8 103 9 to to IN cord-268467-btfz6ye8 103 10 Xray Xray NNP cord-268467-btfz6ye8 103 11 film film NN cord-268467-btfz6ye8 103 12 at at IN cord-268467-btfz6ye8 103 13 -70 -70 NN cord-268467-btfz6ye8 103 14 " " '' cord-268467-btfz6ye8 103 15 . . . cord-268467-btfz6ye8 104 1 Intracellular Intracellular NNP cord-268467-btfz6ye8 104 2 RNA RNA NNP cord-268467-btfz6ye8 104 3 from from IN cord-268467-btfz6ye8 104 4 virus virus NN cord-268467-btfz6ye8 104 5 - - HYPH cord-268467-btfz6ye8 104 6 infected infect VBN cord-268467-btfz6ye8 104 7 cells cell NNS cord-268467-btfz6ye8 104 8 was be VBD cord-268467-btfz6ye8 104 9 denatured denature VBN cord-268467-btfz6ye8 104 10 by by IN cord-268467-btfz6ye8 104 11 glyoxal glyoxal NNP cord-268467-btfz6ye8 104 12 treatment treatment NN cord-268467-btfz6ye8 104 13 and and CC cord-268467-btfz6ye8 104 14 separated separate VBN cord-268467-btfz6ye8 104 15 by by IN cord-268467-btfz6ye8 104 16 electrophoresis electrophoresis NN cord-268467-btfz6ye8 104 17 on on IN cord-268467-btfz6ye8 104 18 a a DT cord-268467-btfz6ye8 104 19 1 1 CD cord-268467-btfz6ye8 104 20 % % NN cord-268467-btfz6ye8 104 21 agarose agarose NN cord-268467-btfz6ye8 104 22 gel gel NN cord-268467-btfz6ye8 104 23 containing contain VBG cord-268467-btfz6ye8 104 24 10 10 CD cord-268467-btfz6ye8 104 25 mll mll NNP cord-268467-btfz6ye8 104 26 / / SYM cord-268467-btfz6ye8 104 27 l l NN cord-268467-btfz6ye8 104 28 sodium sodium NN cord-268467-btfz6ye8 104 29 phosphate phosphate NN cord-268467-btfz6ye8 104 30 ( ( -LRB- cord-268467-btfz6ye8 104 31 pH pH NNP cord-268467-btfz6ye8 104 32 7.0 7.0 CD cord-268467-btfz6ye8 104 33 ) ) -RRB- cord-268467-btfz6ye8 104 34 as as IN cord-268467-btfz6ye8 104 35 described describe VBN cord-268467-btfz6ye8 104 36 previously previously RB cord-268467-btfz6ye8 104 37 ( ( -LRB- cord-268467-btfz6ye8 104 38 Mc Mc NNP cord-268467-btfz6ye8 104 39 - - HYPH cord-268467-btfz6ye8 104 40 Master Master NNP cord-268467-btfz6ye8 104 41 and and CC cord-268467-btfz6ye8 104 42 Carmichael Carmichael NNP cord-268467-btfz6ye8 104 43 , , , cord-268467-btfz6ye8 104 44 1977 1977 CD cord-268467-btfz6ye8 104 45 ) ) -RRB- cord-268467-btfz6ye8 104 46 . . . cord-268467-btfz6ye8 105 1 RNA RNA NNP cord-268467-btfz6ye8 105 2 transfer transfer NN cord-268467-btfz6ye8 105 3 to to IN cord-268467-btfz6ye8 105 4 Biodyne Biodyne NNP cord-268467-btfz6ye8 105 5 nylon nylon NN cord-268467-btfz6ye8 105 6 filters filter NNS cord-268467-btfz6ye8 105 7 ( ( -LRB- cord-268467-btfz6ye8 105 8 ICN ICN NNP cord-268467-btfz6ye8 105 9 Radiochemicals Radiochemicals NNP cord-268467-btfz6ye8 105 10 ) ) -RRB- cord-268467-btfz6ye8 105 11 and and CC cord-268467-btfz6ye8 105 12 subsequent subsequent JJ cord-268467-btfz6ye8 105 13 hybridization hybridization NN cord-268467-btfz6ye8 105 14 were be VBD cord-268467-btfz6ye8 105 15 performed perform VBN cord-268467-btfz6ye8 105 16 according accord VBG cord-268467-btfz6ye8 105 17 to to IN cord-268467-btfz6ye8 105 18 the the DT cord-268467-btfz6ye8 105 19 method method NN cord-268467-btfz6ye8 105 20 described describe VBN cord-268467-btfz6ye8 105 21 by by IN cord-268467-btfz6ye8 105 22 Thomas Thomas NNP cord-268467-btfz6ye8 105 23 ( ( -LRB- cord-268467-btfz6ye8 105 24 1980 1980 CD cord-268467-btfz6ye8 105 25 ) ) -RRB- cord-268467-btfz6ye8 105 26 . . . cord-268467-btfz6ye8 106 1 A a DT cord-268467-btfz6ye8 106 2 synthetic synthetic JJ cord-268467-btfz6ye8 106 3 oligodeoxyribonucleotide oligodeoxyribonucleotide NN cord-268467-btfz6ye8 106 4 was be VBD cord-268467-btfz6ye8 106 5 5'-end 5'-end CD cord-268467-btfz6ye8 106 6 - - HYPH cord-268467-btfz6ye8 106 7 labeled label VBN cord-268467-btfz6ye8 106 8 with with IN cord-268467-btfz6ye8 106 9 [ [ -LRB- cord-268467-btfz6ye8 106 10 Y-~'P]ATP Y-~'P]ATP NNP cord-268467-btfz6ye8 106 11 by by IN cord-268467-btfz6ye8 106 12 polynucleotide polynucleotide NNP cord-268467-btfz6ye8 106 13 kinase kinase NNP cord-268467-btfz6ye8 106 14 ( ( -LRB- cord-268467-btfz6ye8 106 15 Pedersen Pedersen NNP cord-268467-btfz6ye8 106 16 and and CC cord-268467-btfz6ye8 106 17 Haseltine Haseltine NNP cord-268467-btfz6ye8 106 18 , , , cord-268467-btfz6ye8 106 19 1980 1980 CD cord-268467-btfz6ye8 106 20 ) ) -RRB- cord-268467-btfz6ye8 106 21 . . . cord-268467-btfz6ye8 107 1 The the DT cord-268467-btfz6ye8 107 2 total total JJ cord-268467-btfz6ye8 107 3 amount amount NN cord-268467-btfz6ye8 107 4 of of IN cord-268467-btfz6ye8 107 5 poly(A)-containing poly(a)-containe VBG cord-268467-btfz6ye8 107 6 RNA RNA NNP cord-268467-btfz6ye8 107 7 extracted extract VBN cord-268467-btfz6ye8 107 8 from from IN cord-268467-btfz6ye8 107 9 229E 229e CD cord-268467-btfz6ye8 107 10 - - HYPH cord-268467-btfz6ye8 107 11 infected infect VBN cord-268467-btfz6ye8 107 12 cell cell NN cord-268467-btfz6ye8 107 13 monolayers monolayer NNS cord-268467-btfz6ye8 107 14 in in IN cord-268467-btfz6ye8 107 15 three three CD cord-268467-btfz6ye8 107 16 150 150 CD cord-268467-btfz6ye8 107 17 X x NN cord-268467-btfz6ye8 107 18 20-mm 20-mm CD cord-268467-btfz6ye8 107 19 culture culture NN cord-268467-btfz6ye8 107 20 dishes dish NNS cord-268467-btfz6ye8 107 21 was be VBD cord-268467-btfz6ye8 107 22 incubated incubate VBN cord-268467-btfz6ye8 107 23 in in IN cord-268467-btfz6ye8 107 24 8 8 CD cord-268467-btfz6ye8 107 25 PI pi NN cord-268467-btfz6ye8 107 26 of of IN cord-268467-btfz6ye8 107 27 distilled distilled JJ cord-268467-btfz6ye8 107 28 water water NN cord-268467-btfz6ye8 107 29 containing contain VBG cord-268467-btfz6ye8 107 30 10 10 CD cord-268467-btfz6ye8 107 31 mM mm CD cord-268467-btfz6ye8 107 32 methylmercuric methylmercuric JJ cord-268467-btfz6ye8 107 33 hydroxide hydroxide NN cord-268467-btfz6ye8 107 34 for for IN cord-268467-btfz6ye8 107 35 10 10 CD cord-268467-btfz6ye8 107 36 min min NN cord-268467-btfz6ye8 107 37 at at IN cord-268467-btfz6ye8 107 38 room room NN cord-268467-btfz6ye8 107 39 tem tem NN cord-268467-btfz6ye8 107 40 - - HYPH cord-268467-btfz6ye8 107 41 perature perature JJ cord-268467-btfz6ye8 107 42 . . . cord-268467-btfz6ye8 108 1 A a DT cord-268467-btfz6ye8 108 2 further further JJ cord-268467-btfz6ye8 108 3 incubation incubation NN cord-268467-btfz6ye8 108 4 was be VBD cord-268467-btfz6ye8 108 5 carried carry VBN cord-268467-btfz6ye8 108 6 out out RP cord-268467-btfz6ye8 108 7 in in IN cord-268467-btfz6ye8 108 8 a a DT cord-268467-btfz6ye8 108 9 50-~1 50-~1 CD cord-268467-btfz6ye8 108 10 reaction reaction NN cord-268467-btfz6ye8 108 11 volume volume NN cord-268467-btfz6ye8 108 12 containing contain VBG cord-268467-btfz6ye8 108 13 60 60 CD cord-268467-btfz6ye8 108 14 units unit NNS cord-268467-btfz6ye8 108 15 of of IN cord-268467-btfz6ye8 108 16 RNasin RNasin NNP cord-268467-btfz6ye8 108 17 ( ( -LRB- cord-268467-btfz6ye8 108 18 Promega Promega NNP cord-268467-btfz6ye8 108 19 ) ) -RRB- cord-268467-btfz6ye8 108 20 , , , cord-268467-btfz6ye8 108 21 10 10 CD cord-268467-btfz6ye8 108 22 mM mm CD cord-268467-btfz6ye8 108 23 MgC12 MgC12 NNP cord-268467-btfz6ye8 108 24 , , , cord-268467-btfz6ye8 108 25 100 100 CD cord-268467-btfz6ye8 108 26 mM mm CD cord-268467-btfz6ye8 108 27 KCI KCI NNP cord-268467-btfz6ye8 108 28 , , , cord-268467-btfz6ye8 108 29 50 50 CD cord-268467-btfz6ye8 108 30 ml\/l ml\/l NNP cord-268467-btfz6ye8 109 1 Tris Tris NNP cord-268467-btfz6ye8 109 2 - - HYPH cord-268467-btfz6ye8 109 3 HCI HCI NNP cord-268467-btfz6ye8 109 4 ( ( -LRB- cord-268467-btfz6ye8 109 5 pH pH NNP cord-268467-btfz6ye8 109 6 8.3 8.3 CD cord-268467-btfz6ye8 109 7 at at IN cord-268467-btfz6ye8 109 8 42 42 CD cord-268467-btfz6ye8 109 9 " " '' cord-268467-btfz6ye8 109 10 ) ) -RRB- cord-268467-btfz6ye8 110 1 10 10 CD cord-268467-btfz6ye8 110 2 mM mm CD cord-268467-btfz6ye8 110 3 DlT dlt NN cord-268467-btfz6ye8 110 4 , , , cord-268467-btfz6ye8 110 5 1.25 1.25 CD cord-268467-btfz6ye8 110 6 mn mn NNP cord-268467-btfz6ye8 110 7 / / SYM cord-268467-btfz6ye8 110 8 ldNTPs ldNTPs NNP cord-268467-btfz6ye8 110 9 , , , cord-268467-btfz6ye8 110 10 28 28 CD cord-268467-btfz6ye8 110 11 mM mM NNP cord-268467-btfz6ye8 110 12 , , , cord-268467-btfz6ye8 110 13 & & CC cord-268467-btfz6ye8 110 14 mercaptoethanol mercaptoethanol NNP cord-268467-btfz6ye8 110 15 , , , cord-268467-btfz6ye8 110 16 5'-end 5'-end CD cord-268467-btfz6ye8 110 17 - - HYPH cord-268467-btfz6ye8 110 18 labeled label VBN cord-268467-btfz6ye8 110 19 synthetic synthetic JJ cord-268467-btfz6ye8 110 20 oligodeoxyribonucleotides oligodeoxyribonucleotide NNS cord-268467-btfz6ye8 110 21 , , , cord-268467-btfz6ye8 110 22 and and CC cord-268467-btfz6ye8 110 23 20 20 CD cord-268467-btfz6ye8 110 24 units unit NNS cord-268467-btfz6ye8 110 25 of of IN cord-268467-btfz6ye8 110 26 AMV AMV NNP cord-268467-btfz6ye8 110 27 reverse reverse NN cord-268467-btfz6ye8 110 28 transcriptase transcriptase NN cord-268467-btfz6ye8 110 29 ( ( -LRB- cord-268467-btfz6ye8 110 30 Life Life NNP cord-268467-btfz6ye8 110 31 Science Science NNP cord-268467-btfz6ye8 110 32 ) ) -RRB- cord-268467-btfz6ye8 110 33 for for IN cord-268467-btfz6ye8 110 34 1 1 CD cord-268467-btfz6ye8 110 35 hr hr NN cord-268467-btfz6ye8 110 36 at at IN cord-268467-btfz6ye8 110 37 42 42 CD cord-268467-btfz6ye8 110 38 " " '' cord-268467-btfz6ye8 110 39 . . . cord-268467-btfz6ye8 111 1 Reaction reaction NN cord-268467-btfz6ye8 111 2 products product NNS cord-268467-btfz6ye8 111 3 were be VBD cord-268467-btfz6ye8 111 4 extracted extract VBN cord-268467-btfz6ye8 111 5 with with IN cord-268467-btfz6ye8 111 6 phenol phenol NN cord-268467-btfz6ye8 111 7 / / SYM cord-268467-btfz6ye8 111 8 chloroform chloroform NN cord-268467-btfz6ye8 111 9 , , , cord-268467-btfz6ye8 111 10 precipitated precipitate VBN cord-268467-btfz6ye8 111 11 with with IN cord-268467-btfz6ye8 111 12 ethanol ethanol NN cord-268467-btfz6ye8 111 13 , , , cord-268467-btfz6ye8 111 14 and and CC cord-268467-btfz6ye8 111 15 then then RB cord-268467-btfz6ye8 111 16 analyzed analyze VBN cord-268467-btfz6ye8 111 17 by by IN cord-268467-btfz6ye8 111 18 electrophoresis electrophoresis NN cord-268467-btfz6ye8 111 19 on on IN cord-268467-btfz6ye8 111 20 a a DT cord-268467-btfz6ye8 111 21 6 6 CD cord-268467-btfz6ye8 111 22 % % NN cord-268467-btfz6ye8 111 23 polyacrylamide polyacrylamide NN cord-268467-btfz6ye8 111 24 gel gel NN cord-268467-btfz6ye8 111 25 containing contain VBG cord-268467-btfz6ye8 111 26 8.3 8.3 CD cord-268467-btfz6ye8 111 27 M M NNP cord-268467-btfz6ye8 111 28 urea urea NN cord-268467-btfz6ye8 111 29 . . . cord-268467-btfz6ye8 112 1 The the DT cord-268467-btfz6ye8 112 2 primer primer NN cord-268467-btfz6ye8 112 3 - - HYPH cord-268467-btfz6ye8 112 4 extended extended JJ cord-268467-btfz6ye8 112 5 product product NN cord-268467-btfz6ye8 112 6 was be VBD cord-268467-btfz6ye8 112 7 identified identify VBN cord-268467-btfz6ye8 112 8 by by IN cord-268467-btfz6ye8 112 9 autoradiography autoradiography NNP cord-268467-btfz6ye8 112 10 and and CC cord-268467-btfz6ye8 112 11 eluted elute VBN cord-268467-btfz6ye8 112 12 from from IN cord-268467-btfz6ye8 112 13 the the DT cord-268467-btfz6ye8 112 14 gel gel NN cord-268467-btfz6ye8 112 15 according accord VBG cord-268467-btfz6ye8 112 16 to to IN cord-268467-btfz6ye8 112 17 the the DT cord-268467-btfz6ye8 112 18 published publish VBN cord-268467-btfz6ye8 112 19 procedure procedure NN cord-268467-btfz6ye8 112 20 ( ( -LRB- cord-268467-btfz6ye8 112 21 Maxam Maxam NNP cord-268467-btfz6ye8 112 22 and and CC cord-268467-btfz6ye8 112 23 Gilbert Gilbert NNP cord-268467-btfz6ye8 112 24 , , , cord-268467-btfz6ye8 112 25 1977 1977 CD cord-268467-btfz6ye8 112 26 ) ) -RRB- cord-268467-btfz6ye8 112 27 . . . cord-268467-btfz6ye8 113 1 Sequencing sequencing NN cord-268467-btfz6ye8 113 2 was be VBD cord-268467-btfz6ye8 113 3 carried carry VBN cord-268467-btfz6ye8 113 4 out out RP cord-268467-btfz6ye8 113 5 by by IN cord-268467-btfz6ye8 113 6 the the DT cord-268467-btfz6ye8 113 7 dideoxyribonucleotide dideoxyribonucleotide NN cord-268467-btfz6ye8 113 8 chain chain NN cord-268467-btfz6ye8 113 9 termination termination NN cord-268467-btfz6ye8 113 10 method method NN cord-268467-btfz6ye8 113 11 ( ( -LRB- cord-268467-btfz6ye8 113 12 Sanger Sanger NNP cord-268467-btfz6ye8 113 13 et et NNP cord-268467-btfz6ye8 113 14 al al NNP cord-268467-btfz6ye8 113 15 . . NNP cord-268467-btfz6ye8 113 16 , , , cord-268467-btfz6ye8 113 17 1977 1977 CD cord-268467-btfz6ye8 113 18 ) ) -RRB- cord-268467-btfz6ye8 113 19 as as RB cord-268467-btfz6ye8 113 20 well well RB cord-268467-btfz6ye8 113 21 as as IN cord-268467-btfz6ye8 113 22 the the DT cord-268467-btfz6ye8 113 23 chemical chemical JJ cord-268467-btfz6ye8 113 24 modification modification NN cord-268467-btfz6ye8 113 25 procedure procedure NN cord-268467-btfz6ye8 113 26 ( ( -LRB- cord-268467-btfz6ye8 113 27 Maxam Maxam NNP cord-268467-btfz6ye8 113 28 and and CC cord-268467-btfz6ye8 113 29 Gilbert Gilbert NNP cord-268467-btfz6ye8 113 30 , , , cord-268467-btfz6ye8 113 31 1977 1977 CD cord-268467-btfz6ye8 113 32 ) ) -RRB- cord-268467-btfz6ye8 113 33 . . . cord-268467-btfz6ye8 114 1 In in IN cord-268467-btfz6ye8 114 2 the the DT cord-268467-btfz6ye8 114 3 first first JJ cord-268467-btfz6ye8 114 4 method method NN cord-268467-btfz6ye8 114 5 , , , cord-268467-btfz6ye8 114 6 fragments fragment NNS cord-268467-btfz6ye8 114 7 of of IN cord-268467-btfz6ye8 114 8 cDNA cdna NN cord-268467-btfz6ye8 114 9 inserts insert NNS cord-268467-btfz6ye8 114 10 generated generate VBN cord-268467-btfz6ye8 114 11 by by IN cord-268467-btfz6ye8 114 12 various various JJ cord-268467-btfz6ye8 114 13 restriction restriction NN cord-268467-btfz6ye8 114 14 endonucleases endonuclease NNS cord-268467-btfz6ye8 114 15 were be VBD cord-268467-btfz6ye8 114 16 cloned clone VBN cord-268467-btfz6ye8 114 17 into into IN cord-268467-btfz6ye8 114 18 the the DT cord-268467-btfz6ye8 114 19 Ml Ml NNP cord-268467-btfz6ye8 114 20 3 3 CD cord-268467-btfz6ye8 114 21 vectors vector NNS cord-268467-btfz6ye8 114 22 mp18 mp18 NN cord-268467-btfz6ye8 114 23 and and CC cord-268467-btfz6ye8 114 24 mp19 mp19 NNP cord-268467-btfz6ye8 114 25 ( ( -LRB- cord-268467-btfz6ye8 114 26 Messing messing NN cord-268467-btfz6ye8 114 27 and and CC cord-268467-btfz6ye8 114 28 Vierira Vierira NNP cord-268467-btfz6ye8 114 29 , , , cord-268467-btfz6ye8 114 30 1982 1982 CD cord-268467-btfz6ye8 114 31 ) ) -RRB- cord-268467-btfz6ye8 114 32 . . . cord-268467-btfz6ye8 115 1 [ [ -LRB- cord-268467-btfz6ye8 115 2 ( ( -LRB- cord-268467-btfz6ye8 115 3 u-~~S]-dATP u-~~S]-dATP NNP cord-268467-btfz6ye8 115 4 was be VBD cord-268467-btfz6ye8 115 5 used use VBN cord-268467-btfz6ye8 115 6 as as IN cord-268467-btfz6ye8 115 7 a a DT cord-268467-btfz6ye8 115 8 label label NN cord-268467-btfz6ye8 115 9 . . . cord-268467-btfz6ye8 116 1 Sequence sequence NN cord-268467-btfz6ye8 116 2 data datum NNS cord-268467-btfz6ye8 116 3 were be VBD cord-268467-btfz6ye8 116 4 also also RB cord-268467-btfz6ye8 116 5 obtained obtain VBN cord-268467-btfz6ye8 116 6 by by IN cord-268467-btfz6ye8 116 7 chemical chemical NN cord-268467-btfz6ye8 116 8 modification modification NN cord-268467-btfz6ye8 116 9 ( ( -LRB- cord-268467-btfz6ye8 116 10 Maxam Maxam NNP cord-268467-btfz6ye8 116 11 and and CC cord-268467-btfz6ye8 116 12 Gilbert Gilbert NNP cord-268467-btfz6ye8 116 13 , , , cord-268467-btfz6ye8 116 14 1977 1977 CD cord-268467-btfz6ye8 116 15 ) ) -RRB- cord-268467-btfz6ye8 116 16 of of IN cord-268467-btfz6ye8 116 17 various various JJ cord-268467-btfz6ye8 116 18 cDNA cdna NN cord-268467-btfz6ye8 116 19 fragments fragment NNS cord-268467-btfz6ye8 116 20 subcloned subclone VBN cord-268467-btfz6ye8 116 21 into into IN cord-268467-btfz6ye8 116 22 the the DT cord-268467-btfz6ye8 116 23 pT7 pt7 NN cord-268467-btfz6ye8 116 24 - - SYM cord-268467-btfz6ye8 116 25 3 3 CD cord-268467-btfz6ye8 116 26 vector vector NN cord-268467-btfz6ye8 116 27 ( ( -LRB- cord-268467-btfz6ye8 116 28 Tabor Tabor NNP cord-268467-btfz6ye8 116 29 and and CC cord-268467-btfz6ye8 116 30 Richardson Richardson NNP cord-268467-btfz6ye8 116 31 , , , cord-268467-btfz6ye8 116 32 1985 1985 CD cord-268467-btfz6ye8 116 33 ) ) -RRB- cord-268467-btfz6ye8 116 34 . . . cord-268467-btfz6ye8 117 1 In in IN cord-268467-btfz6ye8 117 2 the the DT cord-268467-btfz6ye8 117 3 second second JJ cord-268467-btfz6ye8 117 4 method method NN cord-268467-btfz6ye8 117 5 , , , cord-268467-btfz6ye8 117 6 cDNA cdna NN cord-268467-btfz6ye8 117 7 fragments fragment NNS cord-268467-btfz6ye8 117 8 were be VBD cord-268467-btfz6ye8 117 9 3'-end 3'-end CD cord-268467-btfz6ye8 117 10 - - HYPH cord-268467-btfz6ye8 117 11 labeled label VBN cord-268467-btfz6ye8 117 12 with with IN cord-268467-btfz6ye8 117 13 Klenow Klenow NNP cord-268467-btfz6ye8 117 14 fragment fragment NN cord-268467-btfz6ye8 117 15 at at IN cord-268467-btfz6ye8 117 16 internal internal JJ cord-268467-btfz6ye8 117 17 restriction restriction NN cord-268467-btfz6ye8 117 18 sites site NNS cord-268467-btfz6ye8 117 19 or or CC cord-268467-btfz6ye8 117 20 , , , cord-268467-btfz6ye8 117 21 alternatively alternatively RB cord-268467-btfz6ye8 117 22 , , , cord-268467-btfz6ye8 117 23 at at IN cord-268467-btfz6ye8 117 24 the the DT cord-268467-btfz6ye8 117 25 polylinker polylinker NN cord-268467-btfz6ye8 117 26 cloning cloning NN cord-268467-btfz6ye8 117 27 site site NN cord-268467-btfz6ye8 117 28 of of IN cord-268467-btfz6ye8 117 29 pT7 pt7 NN cord-268467-btfz6ye8 117 30 - - SYM cord-268467-btfz6ye8 117 31 3 3 CD cord-268467-btfz6ye8 117 32 . . . cord-268467-btfz6ye8 118 1 End end NN cord-268467-btfz6ye8 118 2 - - HYPH cord-268467-btfz6ye8 118 3 labeled label VBN cord-268467-btfz6ye8 118 4 cDNA cdna NN cord-268467-btfz6ye8 118 5 restriction restriction NN cord-268467-btfz6ye8 118 6 fragments fragment NNS cord-268467-btfz6ye8 118 7 were be VBD cord-268467-btfz6ye8 118 8 separated separate VBN cord-268467-btfz6ye8 118 9 by by IN cord-268467-btfz6ye8 118 10 electrophoresis electrophoresis NN cord-268467-btfz6ye8 118 11 on on IN cord-268467-btfz6ye8 118 12 preparative preparative JJ cord-268467-btfz6ye8 118 13 polyacrylamide polyacrylamide NN cord-268467-btfz6ye8 118 14 gels gel NNS cord-268467-btfz6ye8 118 15 ( ( -LRB- cord-268467-btfz6ye8 118 16 Maxam Maxam NNP cord-268467-btfz6ye8 118 17 and and CC cord-268467-btfz6ye8 118 18 Gilbert Gilbert NNP cord-268467-btfz6ye8 118 19 , , , cord-268467-btfz6ye8 118 20 1980 1980 CD cord-268467-btfz6ye8 118 21 ) ) -RRB- cord-268467-btfz6ye8 118 22 and and CC cord-268467-btfz6ye8 118 23 purified purify VBN cord-268467-btfz6ye8 118 24 as as IN cord-268467-btfz6ye8 118 25 described describe VBN cord-268467-btfz6ye8 118 26 previously previously RB cord-268467-btfz6ye8 118 27 ( ( -LRB- cord-268467-btfz6ye8 118 28 Hansen Hansen NNP cord-268467-btfz6ye8 118 29 et et NNP cord-268467-btfz6ye8 118 30 a/. a/. NN cord-268467-btfz6ye8 118 31 , , , cord-268467-btfz6ye8 118 32 1980 1980 CD cord-268467-btfz6ye8 118 33 ; ; : cord-268467-btfz6ye8 118 34 Hansen Hansen NNP cord-268467-btfz6ye8 118 35 , , , cord-268467-btfz6ye8 118 36 1981 1981 CD cord-268467-btfz6ye8 118 37 ) ) -RRB- cord-268467-btfz6ye8 118 38 . . . cord-268467-btfz6ye8 119 1 Sequencing sequencing NN cord-268467-btfz6ye8 119 2 of of IN cord-268467-btfz6ye8 119 3 the the DT cord-268467-btfz6ye8 119 4 primer primer NN cord-268467-btfz6ye8 119 5 - - HYPH cord-268467-btfz6ye8 119 6 extended extended JJ cord-268467-btfz6ye8 119 7 product product NN cord-268467-btfz6ye8 119 8 of of IN cord-268467-btfz6ye8 119 9 mRNA7 mrna7 NN cord-268467-btfz6ye8 119 10 was be VBD cord-268467-btfz6ye8 119 11 performed perform VBN cord-268467-btfz6ye8 119 12 by by IN cord-268467-btfz6ye8 119 13 the the DT cord-268467-btfz6ye8 119 14 chemical chemical JJ cord-268467-btfz6ye8 119 15 modification modification NN cord-268467-btfz6ye8 119 16 procedure procedure NN cord-268467-btfz6ye8 119 17 ( ( -LRB- cord-268467-btfz6ye8 119 18 Maxam Maxam NNP cord-268467-btfz6ye8 119 19 and and CC cord-268467-btfz6ye8 119 20 Gilbert Gilbert NNP cord-268467-btfz6ye8 119 21 , , , cord-268467-btfz6ye8 119 22 1977 1977 CD cord-268467-btfz6ye8 119 23 ) ) -RRB- cord-268467-btfz6ye8 119 24 . . . cord-268467-btfz6ye8 120 1 Sequence sequence NN cord-268467-btfz6ye8 120 2 analysis analysis NN cord-268467-btfz6ye8 120 3 was be VBD cord-268467-btfz6ye8 120 4 performed perform VBN cord-268467-btfz6ye8 120 5 by by IN cord-268467-btfz6ye8 120 6 the the DT cord-268467-btfz6ye8 120 7 lntelligenetics lntelligenetic NNS cord-268467-btfz6ye8 120 8 and and CC cord-268467-btfz6ye8 120 9 Seqaid Seqaid NNP cord-268467-btfz6ye8 120 10 programs program NNS cord-268467-btfz6ye8 120 11 . . . cord-268467-btfz6ye8 121 1 Hydropathy Hydropathy NNP cord-268467-btfz6ye8 121 2 profiles profile NNS cord-268467-btfz6ye8 121 3 were be VBD cord-268467-btfz6ye8 121 4 constructed construct VBN cord-268467-btfz6ye8 121 5 using use VBG cord-268467-btfz6ye8 121 6 the the DT cord-268467-btfz6ye8 121 7 PepPlot PepPlot NNP cord-268467-btfz6ye8 121 8 program program NN cord-268467-btfz6ye8 121 9 of of IN cord-268467-btfz6ye8 121 10 the the DT cord-268467-btfz6ye8 121 11 University University NNP cord-268467-btfz6ye8 121 12 of of IN cord-268467-btfz6ye8 121 13 Wisconsin Wisconsin NNP cord-268467-btfz6ye8 121 14 Computer Computer NNP cord-268467-btfz6ye8 121 15 Genetics Genetics NNP cord-268467-btfz6ye8 121 16 Group Group NNP cord-268467-btfz6ye8 121 17 , , , cord-268467-btfz6ye8 121 18 which which WDT cord-268467-btfz6ye8 121 19 employs employ VBZ cord-268467-btfz6ye8 121 20 both both CC cord-268467-btfz6ye8 121 21 the the DT cord-268467-btfz6ye8 121 22 Kyle Kyle NNP cord-268467-btfz6ye8 121 23 - - HYPH cord-268467-btfz6ye8 121 24 Doolittle Doolittle NNP cord-268467-btfz6ye8 121 25 ( ( -LRB- cord-268467-btfz6ye8 121 26 KD KD NNP cord-268467-btfz6ye8 121 27 ) ) -RRB- cord-268467-btfz6ye8 121 28 and and CC cord-268467-btfz6ye8 121 29 Goldman Goldman NNP cord-268467-btfz6ye8 121 30 , , , cord-268467-btfz6ye8 121 31 Engelman Engelman NNP cord-268467-btfz6ye8 121 32 , , , cord-268467-btfz6ye8 121 33 Steitz Steitz NNP cord-268467-btfz6ye8 121 34 ( ( -LRB- cord-268467-btfz6ye8 121 35 GES GES NNP cord-268467-btfz6ye8 121 36 ) ) -RRB- cord-268467-btfz6ye8 121 37 algorithms algorithm NNS cord-268467-btfz6ye8 121 38 . . . cord-268467-btfz6ye8 122 1 To to TO cord-268467-btfz6ye8 122 2 determine determine VB cord-268467-btfz6ye8 122 3 the the DT cord-268467-btfz6ye8 122 4 optimum optimum JJ cord-268467-btfz6ye8 122 5 time time NN cord-268467-btfz6ye8 122 6 for for IN cord-268467-btfz6ye8 122 7 extracting extract VBG cord-268467-btfz6ye8 122 8 229Especific 229especific NN cord-268467-btfz6ye8 122 9 mRNAs mRNAs NNPS cord-268467-btfz6ye8 122 10 , , , cord-268467-btfz6ye8 122 11 we -PRON- PRP cord-268467-btfz6ye8 122 12 first first RB cord-268467-btfz6ye8 122 13 studied study VBD cord-268467-btfz6ye8 122 14 the the DT cord-268467-btfz6ye8 122 15 kinetics kinetic NNS cord-268467-btfz6ye8 122 16 of of IN cord-268467-btfz6ye8 122 17 virusspecific virusspecific NNP cord-268467-btfz6ye8 122 18 mRNA mRNA NNP cord-268467-btfz6ye8 122 19 synthesis synthesis NN cord-268467-btfz6ye8 122 20 . . . cord-268467-btfz6ye8 123 1 Intracellular Intracellular NNP cord-268467-btfz6ye8 123 2 RNA RNA NNP cord-268467-btfz6ye8 123 3 was be VBD cord-268467-btfz6ye8 123 4 extracted extract VBN cord-268467-btfz6ye8 123 5 from from IN cord-268467-btfz6ye8 123 6 infected infected JJ cord-268467-btfz6ye8 123 7 L132 L132 NNP cord-268467-btfz6ye8 123 8 monolayers monolayer NNS cord-268467-btfz6ye8 123 9 at at IN cord-268467-btfz6ye8 123 10 specified specify VBN cord-268467-btfz6ye8 123 11 times time NNS cord-268467-btfz6ye8 123 12 p.i p.i NNP cord-268467-btfz6ye8 123 13 . . . cord-268467-btfz6ye8 124 1 The the DT cord-268467-btfz6ye8 124 2 RNA RNA NNP cord-268467-btfz6ye8 124 3 was be VBD cord-268467-btfz6ye8 124 4 separated separate VBN cord-268467-btfz6ye8 124 5 by by IN cord-268467-btfz6ye8 124 6 agarose agarose JJ cord-268467-btfz6ye8 124 7 gel gel NN cord-268467-btfz6ye8 124 8 electrophoresis electrophoresis NN cord-268467-btfz6ye8 124 9 ( ( -LRB- cord-268467-btfz6ye8 124 10 Fig Fig NNP cord-268467-btfz6ye8 124 11 . . . cord-268467-btfz6ye8 124 12 1 1 CD cord-268467-btfz6ye8 124 13 ) ) -RRB- cord-268467-btfz6ye8 124 14 . . . cord-268467-btfz6ye8 125 1 As as IN cord-268467-btfz6ye8 125 2 can can MD cord-268467-btfz6ye8 125 3 be be VB cord-268467-btfz6ye8 125 4 seen see VBN cord-268467-btfz6ye8 125 5 , , , cord-268467-btfz6ye8 125 6 viral viral JJ cord-268467-btfz6ye8 125 7 mRNA mRNA NNP cord-268467-btfz6ye8 125 8 synthesis synthesis NN cord-268467-btfz6ye8 125 9 could could MD cord-268467-btfz6ye8 125 10 be be VB cord-268467-btfz6ye8 125 11 detected detect VBN cord-268467-btfz6ye8 125 12 as as RB cord-268467-btfz6ye8 125 13 early early RB cord-268467-btfz6ye8 125 14 as as IN cord-268467-btfz6ye8 125 15 7 7 CD cord-268467-btfz6ye8 125 16 hr hr NN cord-268467-btfz6ye8 125 17 p.i p.i NNP cord-268467-btfz6ye8 125 18 . . NNP cord-268467-btfz6ye8 125 19 and and CC cord-268467-btfz6ye8 125 20 reached reach VBD cord-268467-btfz6ye8 125 21 maximum maximum NN cord-268467-btfz6ye8 125 22 at at IN cord-268467-btfz6ye8 125 23 29 29 CD cord-268467-btfz6ye8 125 24 hr hr NN cord-268467-btfz6ye8 125 25 p.i p.i NNP cord-268467-btfz6ye8 125 26 . . . cord-268467-btfz6ye8 126 1 Thereafter thereafter RB cord-268467-btfz6ye8 126 2 , , , cord-268467-btfz6ye8 126 3 total total JJ cord-268467-btfz6ye8 126 4 RNA RNA NNP cord-268467-btfz6ye8 126 5 synthesis synthesis NN cord-268467-btfz6ye8 126 6 gradually gradually RB cord-268467-btfz6ye8 126 7 declined decline VBD cord-268467-btfz6ye8 126 8 . . . cord-268467-btfz6ye8 127 1 By by IN cord-268467-btfz6ye8 127 2 46 46 CD cord-268467-btfz6ye8 127 3 hr hr NN cord-268467-btfz6ye8 127 4 p.i p.i NNP cord-268467-btfz6ye8 127 5 . . NNP cord-268467-btfz6ye8 127 6 onlythe onlythe NNP cord-268467-btfz6ye8 127 7 most most RBS cord-268467-btfz6ye8 127 8 abundant abundant JJ cord-268467-btfz6ye8 127 9 mRNA mRNA NNP cord-268467-btfz6ye8 127 10 species specie NNS cord-268467-btfz6ye8 127 11 were be VBD cord-268467-btfz6ye8 127 12 evident evident JJ cord-268467-btfz6ye8 127 13 . . . cord-268467-btfz6ye8 128 1 The the DT cord-268467-btfz6ye8 128 2 number number NN cord-268467-btfz6ye8 128 3 and and CC cord-268467-btfz6ye8 128 4 size size NN cord-268467-btfz6ye8 128 5 of of IN cord-268467-btfz6ye8 128 6 these these DT cord-268467-btfz6ye8 128 7 mRNA mRNA NNP cord-268467-btfz6ye8 128 8 species specie NNS cord-268467-btfz6ye8 128 9 are be VBP cord-268467-btfz6ye8 128 10 comparable comparable JJ cord-268467-btfz6ye8 128 11 to to IN cord-268467-btfz6ye8 128 12 those those DT cord-268467-btfz6ye8 128 13 of of IN cord-268467-btfz6ye8 128 14 MHV MHV NNP cord-268467-btfz6ye8 128 15 mRNAs mRNAs NNPS cord-268467-btfz6ye8 128 16 and and CC cord-268467-btfz6ye8 128 17 are be VBP cord-268467-btfz6ye8 128 18 in in IN cord-268467-btfz6ye8 128 19 agreement agreement NN cord-268467-btfz6ye8 128 20 with with IN cord-268467-btfz6ye8 128 21 previously previously RB cord-268467-btfz6ye8 128 22 published publish VBN cord-268467-btfz6ye8 128 23 results result NNS cord-268467-btfz6ye8 128 24 ( ( -LRB- cord-268467-btfz6ye8 128 25 Weiss Weiss NNP cord-268467-btfz6ye8 128 26 and and CC cord-268467-btfz6ye8 128 27 Leibowitz Leibowitz NNP cord-268467-btfz6ye8 128 28 , , , cord-268467-btfz6ye8 128 29 1981 1981 CD cord-268467-btfz6ye8 128 30 ) ) -RRB- cord-268467-btfz6ye8 128 31 . . . cord-268467-btfz6ye8 129 1 Significantly significantly RB cord-268467-btfz6ye8 129 2 , , , cord-268467-btfz6ye8 129 3 mRNA mRNA NNP cord-268467-btfz6ye8 129 4 2a 2a CD cord-268467-btfz6ye8 129 5 , , , cord-268467-btfz6ye8 129 6 which which WDT cord-268467-btfz6ye8 129 7 was be VBD cord-268467-btfz6ye8 129 8 previously previously RB cord-268467-btfz6ye8 129 9 found find VBN cord-268467-btfz6ye8 129 10 only only RB cord-268467-btfz6ye8 129 11 in in IN cord-268467-btfz6ye8 129 12 BCV BCV NNP cord-268467-btfz6ye8 129 13 - - HYPH cord-268467-btfz6ye8 129 14 infected infect VBN cord-268467-btfz6ye8 129 15 cells cell NNS cord-268467-btfz6ye8 129 16 and and CC cord-268467-btfz6ye8 129 17 proposed propose VBD cord-268467-btfz6ye8 129 18 to to TO cord-268467-btfz6ye8 129 19 encode encode VB cord-268467-btfz6ye8 129 20 hemagglutinins hemagglutinins NNP cord-268467-btfz6ye8 129 21 ( ( -LRB- cord-268467-btfz6ye8 129 22 King King NNP cord-268467-btfz6ye8 129 23 et et NNP cord-268467-btfz6ye8 129 24 a/. a/. NNP cord-268467-btfz6ye8 129 25 , , , cord-268467-btfz6ye8 129 26 1985 1985 CD cord-268467-btfz6ye8 129 27 ; ; : cord-268467-btfz6ye8 129 28 Keck Keck NNP cord-268467-btfz6ye8 129 29 et et NNP cord-268467-btfz6ye8 129 30 a/. a/. NNP cord-268467-btfz6ye8 129 31 , , , cord-268467-btfz6ye8 129 32 1988 1988 CD cord-268467-btfz6ye8 129 33 ) ) -RRB- cord-268467-btfz6ye8 129 34 was be VBD cord-268467-btfz6ye8 129 35 not not RB cord-268467-btfz6ye8 129 36 present present JJ cord-268467-btfz6ye8 129 37 . . . cord-268467-btfz6ye8 130 1 This this DT cord-268467-btfz6ye8 130 2 is be VBZ cord-268467-btfz6ye8 130 3 consistent consistent JJ cord-268467-btfz6ye8 130 4 with with IN cord-268467-btfz6ye8 130 5 the the DT cord-268467-btfz6ye8 130 6 finding finding NN cord-268467-btfz6ye8 130 7 that that WDT cord-268467-btfz6ye8 130 8 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 130 9 does do VBZ cord-268467-btfz6ye8 130 10 not not RB cord-268467-btfz6ye8 130 11 have have VB cord-268467-btfz6ye8 130 12 hemagglutinating hemagglutinate VBG cord-268467-btfz6ye8 130 13 activity activity NN cord-268467-btfz6ye8 130 14 ( ( -LRB- cord-268467-btfz6ye8 130 15 Hierholzer Hierholzer NNP cord-268467-btfz6ye8 130 16 , , , cord-268467-btfz6ye8 130 17 1976 1976 CD cord-268467-btfz6ye8 130 18 ) ) -RRB- cord-268467-btfz6ye8 130 19 . . . cord-268467-btfz6ye8 131 1 The the DT cord-268467-btfz6ye8 131 2 relative relative JJ cord-268467-btfz6ye8 131 3 amounts amount NNS cord-268467-btfz6ye8 131 4 of of IN cord-268467-btfz6ye8 131 5 the the DT cord-268467-btfz6ye8 131 6 mRNA mRNA NNP cord-268467-btfz6ye8 131 7 species specie NNS cord-268467-btfz6ye8 131 8 were be VBD cord-268467-btfz6ye8 131 9 the the DT cord-268467-btfz6ye8 131 10 same same JJ cord-268467-btfz6ye8 131 11 throughout throughout IN cord-268467-btfz6ye8 131 12 the the DT cord-268467-btfz6ye8 131 13 replication replication NN cord-268467-btfz6ye8 131 14 cycle cycle NN cord-268467-btfz6ye8 131 15 . . . cord-268467-btfz6ye8 132 1 Therefore therefore RB cord-268467-btfz6ye8 132 2 , , , cord-268467-btfz6ye8 132 3 in in IN cord-268467-btfz6ye8 132 4 all all DT cord-268467-btfz6ye8 132 5 of of IN cord-268467-btfz6ye8 132 6 our -PRON- PRP$ cord-268467-btfz6ye8 132 7 subsequent subsequent JJ cord-268467-btfz6ye8 132 8 experiments experiment NNS cord-268467-btfz6ye8 132 9 , , , cord-268467-btfz6ye8 132 10 the the DT cord-268467-btfz6ye8 132 11 virus virus NN cord-268467-btfz6ye8 132 12 - - HYPH cord-268467-btfz6ye8 132 13 specific specific JJ cord-268467-btfz6ye8 132 14 intracellular intracellular JJ cord-268467-btfz6ye8 132 15 RNAs rna NNS cord-268467-btfz6ye8 132 16 were be VBD cord-268467-btfz6ye8 132 17 extracted extract VBN cord-268467-btfz6ye8 132 18 at at IN cord-268467-btfz6ye8 132 19 15 15 CD cord-268467-btfz6ye8 132 20 hr hr NN cord-268467-btfz6ye8 132 21 p.i p.i NNP cord-268467-btfz6ye8 132 22 . . . cord-268467-btfz6ye8 132 23 Molecular molecular JJ cord-268467-btfz6ye8 132 24 cloning cloning NN cord-268467-btfz6ye8 132 25 of of IN cord-268467-btfz6ye8 132 26 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 132 27 genomic genomic JJ cord-268467-btfz6ye8 132 28 RNA RNA NNP cord-268467-btfz6ye8 132 29 and and CC cord-268467-btfz6ye8 132 30 intracellular intracellular JJ cord-268467-btfz6ye8 132 31 virus virus NN cord-268467-btfz6ye8 132 32 - - HYPH cord-268467-btfz6ye8 132 33 specific specific JJ cord-268467-btfz6ye8 132 34 mRNAs mrnas POS cord-268467-btfz6ye8 132 35 cDNA cdna NN cord-268467-btfz6ye8 132 36 cloning cloning NN cord-268467-btfz6ye8 132 37 was be VBD cord-268467-btfz6ye8 132 38 initially initially RB cord-268467-btfz6ye8 132 39 performed perform VBN cord-268467-btfz6ye8 132 40 using use VBG cord-268467-btfz6ye8 132 41 virion virion NN cord-268467-btfz6ye8 132 42 genomic genomic JJ cord-268467-btfz6ye8 132 43 RNA RNA NNP cord-268467-btfz6ye8 132 44 as as IN cord-268467-btfz6ye8 132 45 a a DT cord-268467-btfz6ye8 132 46 template template NN cord-268467-btfz6ye8 132 47 . . . cord-268467-btfz6ye8 133 1 The the DT cord-268467-btfz6ye8 133 2 sizes size NNS cord-268467-btfz6ye8 133 3 of of IN cord-268467-btfz6ye8 133 4 inserts insert NNS cord-268467-btfz6ye8 133 5 in in IN cord-268467-btfz6ye8 133 6 the the DT cord-268467-btfz6ye8 133 7 resultant resultant JJ cord-268467-btfz6ye8 133 8 cDNA cdna NN cord-268467-btfz6ye8 133 9 clones clone NNS cord-268467-btfz6ye8 133 10 ranged range VBD cord-268467-btfz6ye8 133 11 from from IN cord-268467-btfz6ye8 133 12 0.2 0.2 CD cord-268467-btfz6ye8 133 13 to to IN cord-268467-btfz6ye8 133 14 0.5 0.5 CD cord-268467-btfz6ye8 133 15 kb kb NNS cord-268467-btfz6ye8 133 16 in in IN cord-268467-btfz6ye8 133 17 length length NN cord-268467-btfz6ye8 133 18 . . . cord-268467-btfz6ye8 134 1 One one CD cord-268467-btfz6ye8 134 2 clone clone NN cord-268467-btfz6ye8 134 3 , , , cord-268467-btfz6ye8 134 4 A34 A34 NNP cord-268467-btfz6ye8 134 5 , , , cord-268467-btfz6ye8 134 6 contained contain VBD cord-268467-btfz6ye8 134 7 a a DT cord-268467-btfz6ye8 134 8 0.45-kb 0.45-kb CD cord-268467-btfz6ye8 134 9 insert insert NN cord-268467-btfz6ye8 134 10 , , , cord-268467-btfz6ye8 134 11 which which WDT cord-268467-btfz6ye8 134 12 was be VBD cord-268467-btfz6ye8 134 13 subsequently subsequently RB cord-268467-btfz6ye8 134 14 characterized characterize VBN cord-268467-btfz6ye8 134 15 by by IN cord-268467-btfz6ye8 134 16 restriction restriction NN cord-268467-btfz6ye8 134 17 mapping mapping NN cord-268467-btfz6ye8 134 18 and and CC cord-268467-btfz6ye8 134 19 Northern northern JJ cord-268467-btfz6ye8 134 20 blot blot NN cord-268467-btfz6ye8 134 21 analysis analysis NN cord-268467-btfz6ye8 134 22 . . . cord-268467-btfz6ye8 135 1 The the DT cord-268467-btfz6ye8 135 2 0.45 0.45 CD cord-268467-btfz6ye8 135 3 kb kb NNP cord-268467-btfz6ye8 135 4 fragment fragment NN cord-268467-btfz6ye8 135 5 was be VBD cord-268467-btfz6ye8 135 6 labeled label VBN cord-268467-btfz6ye8 135 7 with with IN cord-268467-btfz6ye8 135 8 32P 32p CD cord-268467-btfz6ye8 135 9 by by IN cord-268467-btfz6ye8 135 10 nick nick NNP cord-268467-btfz6ye8 135 11 - - HYPH cord-268467-btfz6ye8 135 12 translation translation NNP cord-268467-btfz6ye8 135 13 and and CC cord-268467-btfz6ye8 135 14 hybridized hybridize VBN cord-268467-btfz6ye8 135 15 with with IN cord-268467-btfz6ye8 135 16 intracellular intracellular JJ cord-268467-btfz6ye8 135 17 RNA RNA NNP cord-268467-btfz6ye8 135 18 from from IN cord-268467-btfz6ye8 135 19 229E 229e CD cord-268467-btfz6ye8 135 20 - - HYPH cord-268467-btfz6ye8 135 21 infected infect VBN cord-268467-btfz6ye8 135 22 cells cell NNS cord-268467-btfz6ye8 135 23 . . . cord-268467-btfz6ye8 136 1 The the DT cord-268467-btfz6ye8 136 2 result result NN cord-268467-btfz6ye8 136 3 , , , cord-268467-btfz6ye8 136 4 shown show VBN cord-268467-btfz6ye8 136 5 in in IN cord-268467-btfz6ye8 136 6 Fig Fig NNP cord-268467-btfz6ye8 136 7 . . NNP cord-268467-btfz6ye8 136 8 2 2 CD cord-268467-btfz6ye8 136 9 , , , cord-268467-btfz6ye8 136 10 revealed reveal VBD cord-268467-btfz6ye8 136 11 that that IN cord-268467-btfz6ye8 136 12 the the DT cord-268467-btfz6ye8 136 13 fragment fragment NN cord-268467-btfz6ye8 136 14 hybridized hybridize VBN cord-268467-btfz6ye8 136 15 to to IN cord-268467-btfz6ye8 136 16 each each DT cord-268467-btfz6ye8 136 17 of of IN cord-268467-btfz6ye8 136 18 the the DT cord-268467-btfz6ye8 136 19 mRNA mRNA NNP cord-268467-btfz6ye8 136 20 species specie NNS cord-268467-btfz6ye8 136 21 . . . cord-268467-btfz6ye8 137 1 This this DT cord-268467-btfz6ye8 137 2 result result NN cord-268467-btfz6ye8 137 3 suggested suggest VBD cord-268467-btfz6ye8 137 4 that that IN cord-268467-btfz6ye8 137 5 the the DT cord-268467-btfz6ye8 137 6 HCV229E HCV229E NNP cord-268467-btfz6ye8 137 7 subgenomic subgenomic NNP cord-268467-btfz6ye8 137 8 mRNAs mRNAs NNPS cord-268467-btfz6ye8 137 9 possess possess VBP cord-268467-btfz6ye8 137 10 a a DT cord-268467-btfz6ye8 137 11 nested nest VBN cord-268467-btfz6ye8 137 12 - - HYPH cord-268467-btfz6ye8 137 13 set set VBN cord-268467-btfz6ye8 137 14 structure structure NN cord-268467-btfz6ye8 137 15 similar similar JJ cord-268467-btfz6ye8 137 16 to to IN cord-268467-btfz6ye8 137 17 other other JJ cord-268467-btfz6ye8 137 18 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 137 19 and and CC cord-268467-btfz6ye8 137 20 that that IN cord-268467-btfz6ye8 137 21 A34 A34 NNP cord-268467-btfz6ye8 137 22 represented represent VBD cord-268467-btfz6ye8 137 23 a a DT cord-268467-btfz6ye8 137 24 cDNA cdna NN cord-268467-btfz6ye8 137 25 clone clone NN cord-268467-btfz6ye8 137 26 of of IN cord-268467-btfz6ye8 137 27 either either CC cord-268467-btfz6ye8 137 28 the the DT cord-268467-btfz6ye8 137 29 3'-end 3'-end CD cord-268467-btfz6ye8 137 30 of of IN cord-268467-btfz6ye8 137 31 the the DT cord-268467-btfz6ye8 137 32 genomic genomic JJ cord-268467-btfz6ye8 137 33 RNA RNA NNP cord-268467-btfz6ye8 137 34 or or CC cord-268467-btfz6ye8 137 35 the the DT cord-268467-btfz6ye8 137 36 leader leader NN cord-268467-btfz6ye8 137 37 sequence sequence NN cord-268467-btfz6ye8 137 38 . . . cord-268467-btfz6ye8 138 1 Cloning clone VBG cord-268467-btfz6ye8 138 2 was be VBD cord-268467-btfz6ye8 138 3 subsequently subsequently RB cord-268467-btfz6ye8 138 4 carried carry VBN cord-268467-btfz6ye8 138 5 out out RP cord-268467-btfz6ye8 138 6 using use VBG cord-268467-btfz6ye8 138 7 intracellular intracellular JJ cord-268467-btfz6ye8 138 8 RNA RNA NNP cord-268467-btfz6ye8 138 9 from from IN cord-268467-btfz6ye8 138 10 229E 229e CD cord-268467-btfz6ye8 138 11 - - HYPH cord-268467-btfz6ye8 138 12 infected infect VBN cord-268467-btfz6ye8 138 13 cells cell NNS cord-268467-btfz6ye8 138 14 as as IN cord-268467-btfz6ye8 138 15 a a DT cord-268467-btfz6ye8 138 16 template template NN cord-268467-btfz6ye8 138 17 . . . cord-268467-btfz6ye8 139 1 The the DT cord-268467-btfz6ye8 139 2 resulting result VBG cord-268467-btfz6ye8 139 3 cDNA cdna NN cord-268467-btfz6ye8 139 4 clones clone NNS cord-268467-btfz6ye8 139 5 were be VBD cord-268467-btfz6ye8 139 6 screened screen VBN cord-268467-btfz6ye8 139 7 by by IN cord-268467-btfz6ye8 139 8 colony colony NN cord-268467-btfz6ye8 139 9 hybridization hybridization NN cord-268467-btfz6ye8 139 10 using use VBG cord-268467-btfz6ye8 139 11 the the DT cord-268467-btfz6ye8 139 12 0.45-kb 0.45-kb CD cord-268467-btfz6ye8 139 13 fragment fragment NN cord-268467-btfz6ye8 139 14 from from IN cord-268467-btfz6ye8 139 15 clone clone NN cord-268467-btfz6ye8 139 16 A34 A34 NNP cord-268467-btfz6ye8 139 17 as as IN cord-268467-btfz6ye8 139 18 a a DT cord-268467-btfz6ye8 139 19 nick nick NNP cord-268467-btfz6ye8 139 20 - - HYPH cord-268467-btfz6ye8 139 21 translated translate VBN cord-268467-btfz6ye8 139 22 probe probe NN cord-268467-btfz6ye8 139 23 ( ( -LRB- cord-268467-btfz6ye8 139 24 Fig Fig NNP cord-268467-btfz6ye8 139 25 . . . cord-268467-btfz6ye8 139 26 3 3 CD cord-268467-btfz6ye8 139 27 ) ) -RRB- cord-268467-btfz6ye8 139 28 . . . cord-268467-btfz6ye8 140 1 Several several JJ cord-268467-btfz6ye8 140 2 positive positive JJ cord-268467-btfz6ye8 140 3 colonies colony NNS cord-268467-btfz6ye8 140 4 were be VBD cord-268467-btfz6ye8 140 5 identified identify VBN cord-268467-btfz6ye8 140 6 and and CC cord-268467-btfz6ye8 140 7 characterized characterize VBN cord-268467-btfz6ye8 140 8 further further RB cord-268467-btfz6ye8 140 9 . . . cord-268467-btfz6ye8 141 1 Clone Clone NNP cord-268467-btfz6ye8 141 2 L8 L8 NNP cord-268467-btfz6ye8 141 3 contained contain VBD cord-268467-btfz6ye8 141 4 a a DT cord-268467-btfz6ye8 141 5 3.6-kb 3.6-kb CD cord-268467-btfz6ye8 141 6 insert insert NN cord-268467-btfz6ye8 141 7 but but CC cord-268467-btfz6ye8 141 8 lacked lack VBD cord-268467-btfz6ye8 141 9 a a DT cord-268467-btfz6ye8 141 10 3'-poly(A 3'-poly(a CD cord-268467-btfz6ye8 141 11 ) ) -RRB- cord-268467-btfz6ye8 141 12 tail tail NN cord-268467-btfz6ye8 141 13 . . . cord-268467-btfz6ye8 142 1 Clone Clone NNP cord-268467-btfz6ye8 142 2 L37 L37 NNP cord-268467-btfz6ye8 142 3 , , , cord-268467-btfz6ye8 142 4 which which WDT cord-268467-btfz6ye8 142 5 contained contain VBD cord-268467-btfz6ye8 142 6 an an DT cord-268467-btfz6ye8 142 7 insert insert NN cord-268467-btfz6ye8 142 8 of of IN cord-268467-btfz6ye8 142 9 1.7 1.7 CD cord-268467-btfz6ye8 142 10 kb kb NNP cord-268467-btfz6ye8 142 11 , , , cord-268467-btfz6ye8 142 12 overlapped overlap VBD cord-268467-btfz6ye8 142 13 L8 L8 NNP cord-268467-btfz6ye8 142 14 but but CC cord-268467-btfz6ye8 142 15 was be VBD cord-268467-btfz6ye8 142 16 0.1 0.1 CD cord-268467-btfz6ye8 142 17 kb kb XX cord-268467-btfz6ye8 142 18 shorter short JJR cord-268467-btfz6ye8 142 19 at at IN cord-268467-btfz6ye8 142 20 the the DT cord-268467-btfz6ye8 142 21 3'-end 3'-end CD cord-268467-btfz6ye8 142 22 . . . cord-268467-btfz6ye8 143 1 This this DT cord-268467-btfz6ye8 143 2 clone clone NN cord-268467-btfz6ye8 143 3 also also RB cord-268467-btfz6ye8 143 4 lacked lack VBD cord-268467-btfz6ye8 143 5 a a DT cord-268467-btfz6ye8 143 6 poly(A poly(a JJ cord-268467-btfz6ye8 143 7 ) ) -RRB- cord-268467-btfz6ye8 143 8 sequence sequence NN cord-268467-btfz6ye8 143 9 ( ( -LRB- cord-268467-btfz6ye8 143 10 see see VB cord-268467-btfz6ye8 143 11 below below RB cord-268467-btfz6ye8 143 12 ) ) -RRB- cord-268467-btfz6ye8 143 13 . . . cord-268467-btfz6ye8 144 1 Therefore therefore RB cord-268467-btfz6ye8 144 2 , , , cord-268467-btfz6ye8 144 3 additional additional JJ cord-268467-btfz6ye8 144 4 cDNA cdna NN cord-268467-btfz6ye8 144 5 clones clone NNS cord-268467-btfz6ye8 144 6 were be VBD cord-268467-btfz6ye8 144 7 isolated isolate VBN cord-268467-btfz6ye8 144 8 using use VBG cord-268467-btfz6ye8 144 9 a a DT cord-268467-btfz6ye8 144 10 0.24-kb 0.24-kb CD cord-268467-btfz6ye8 144 11 Bal Bal NNP cord-268467-btfz6ye8 144 12 I i NN cord-268467-btfz6ye8 144 13 - - HYPH cord-268467-btfz6ye8 144 14 EcoRI ecori NN cord-268467-btfz6ye8 144 15 fragment fragment NN cord-268467-btfz6ye8 144 16 of of IN cord-268467-btfz6ye8 144 17 L8 L8 NNP cord-268467-btfz6ye8 144 18 ( ( -LRB- cord-268467-btfz6ye8 144 19 Fig Fig NNP cord-268467-btfz6ye8 144 20 . . . cord-268467-btfz6ye8 144 21 3a 3a LS cord-268467-btfz6ye8 144 22 ) ) -RRB- cord-268467-btfz6ye8 144 23 as as IN cord-268467-btfz6ye8 144 24 a a DT cord-268467-btfz6ye8 144 25 probe probe NN cord-268467-btfz6ye8 144 26 . . . cord-268467-btfz6ye8 145 1 These these DT cord-268467-btfz6ye8 145 2 latter latter JJ cord-268467-btfz6ye8 145 3 clones clone NNS cord-268467-btfz6ye8 145 4 were be VBD cord-268467-btfz6ye8 145 5 further further RB cord-268467-btfz6ye8 145 6 characterized characterize VBN cord-268467-btfz6ye8 145 7 by by IN cord-268467-btfz6ye8 145 8 Southern southern JJ cord-268467-btfz6ye8 145 9 blot blot NN cord-268467-btfz6ye8 145 10 analysis analysis NN cord-268467-btfz6ye8 145 11 . . . cord-268467-btfz6ye8 146 1 Clone clone NN cord-268467-btfz6ye8 146 2 SlO slo NN cord-268467-btfz6ye8 146 3 contained contain VBD cord-268467-btfz6ye8 146 4 an an DT cord-268467-btfz6ye8 146 5 insert insert NN cord-268467-btfz6ye8 146 6 of of IN cord-268467-btfz6ye8 146 7 0.8 0.8 CD cord-268467-btfz6ye8 146 8 kb kb NNP cord-268467-btfz6ye8 146 9 which which WDT cord-268467-btfz6ye8 146 10 overlapped overlap VBD cord-268467-btfz6ye8 146 11 the the DT cord-268467-btfz6ye8 146 12 3'-ends 3'-ends CD cord-268467-btfz6ye8 146 13 of of IN cord-268467-btfz6ye8 146 14 the the DT cord-268467-btfz6ye8 146 15 two two CD cord-268467-btfz6ye8 146 16 previous previous JJ cord-268467-btfz6ye8 146 17 clones clone NNS cord-268467-btfz6ye8 146 18 and and CC cord-268467-btfz6ye8 146 19 extended extend VBD cord-268467-btfz6ye8 146 20 another another DT cord-268467-btfz6ye8 146 21 0.4 0.4 CD cord-268467-btfz6ye8 146 22 kb kb NNS cord-268467-btfz6ye8 146 23 in in IN cord-268467-btfz6ye8 146 24 that that DT cord-268467-btfz6ye8 146 25 direction direction NN cord-268467-btfz6ye8 146 26 . . . cord-268467-btfz6ye8 147 1 Figure figure NN cord-268467-btfz6ye8 147 2 3b 3b CD cord-268467-btfz6ye8 147 3 shows show VBZ cord-268467-btfz6ye8 147 4 the the DT cord-268467-btfz6ye8 147 5 orientation orientation NN cord-268467-btfz6ye8 147 6 and and CC cord-268467-btfz6ye8 147 7 sizes size NNS cord-268467-btfz6ye8 147 8 of of IN cord-268467-btfz6ye8 147 9 clones clone NNS cord-268467-btfz6ye8 147 10 L8 L8 NNP cord-268467-btfz6ye8 147 11 , , , cord-268467-btfz6ye8 147 12 L37 L37 NNP cord-268467-btfz6ye8 147 13 , , , cord-268467-btfz6ye8 147 14 Sl Sl NNP cord-268467-btfz6ye8 147 15 0 0 CD cord-268467-btfz6ye8 147 16 , , , cord-268467-btfz6ye8 147 17 and and CC cord-268467-btfz6ye8 147 18 A34 A34 NNP cord-268467-btfz6ye8 147 19 with with IN cord-268467-btfz6ye8 147 20 reference reference NN cord-268467-btfz6ye8 147 21 to to IN cord-268467-btfz6ye8 147 22 theviral theviral NNP cord-268467-btfz6ye8 147 23 genome genome NNP cord-268467-btfz6ye8 147 24 . . . cord-268467-btfz6ye8 148 1 Restriction restriction NN cord-268467-btfz6ye8 148 2 enzyme enzyme NN cord-268467-btfz6ye8 148 3 sites site NNS cord-268467-btfz6ye8 148 4 used use VBN cord-268467-btfz6ye8 148 5 for for IN cord-268467-btfz6ye8 148 6 sequencing sequencing NN cord-268467-btfz6ye8 148 7 are be VBP cord-268467-btfz6ye8 148 8 also also RB cord-268467-btfz6ye8 148 9 shown show VBN cord-268467-btfz6ye8 148 10 . . . cord-268467-btfz6ye8 149 1 To to TO cord-268467-btfz6ye8 149 2 determine determine VB cord-268467-btfz6ye8 149 3 the the DT cord-268467-btfz6ye8 149 4 sequence sequence NN cord-268467-btfz6ye8 149 5 of of IN cord-268467-btfz6ye8 149 6 the the DT cord-268467-btfz6ye8 149 7 3'-end 3'-end CD cord-268467-btfz6ye8 149 8 of of IN cord-268467-btfz6ye8 149 9 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 149 10 genome genome NN cord-268467-btfz6ye8 149 11 , , , cord-268467-btfz6ye8 149 12 various various JJ cord-268467-btfz6ye8 149 13 restriction restriction NN cord-268467-btfz6ye8 149 14 fragments fragment NNS cord-268467-btfz6ye8 149 15 of of IN cord-268467-btfz6ye8 149 16 L8 L8 NNP cord-268467-btfz6ye8 149 17 , , , cord-268467-btfz6ye8 149 18 L37 L37 NNP cord-268467-btfz6ye8 149 19 , , , cord-268467-btfz6ye8 149 20 and and CC cord-268467-btfz6ye8 149 21 SlO slo NN cord-268467-btfz6ye8 149 22 were be VBD cord-268467-btfz6ye8 149 23 subcloned subclone VBN cord-268467-btfz6ye8 149 24 into into IN cord-268467-btfz6ye8 149 25 Ml Ml NNP cord-268467-btfz6ye8 149 26 3 3 CD cord-268467-btfz6ye8 149 27 vectors vector NNS cord-268467-btfz6ye8 149 28 . . . cord-268467-btfz6ye8 150 1 For for IN cord-268467-btfz6ye8 150 2 L8 L8 NNP cord-268467-btfz6ye8 150 3 , , , cord-268467-btfz6ye8 150 4 only only RB cord-268467-btfz6ye8 150 5 the the DT cord-268467-btfz6ye8 150 6 1.2-kb 1.2-kb CD cord-268467-btfz6ye8 150 7 fragment fragment NN cord-268467-btfz6ye8 150 8 extending extend VBG cord-268467-btfz6ye8 150 9 from from IN cord-268467-btfz6ye8 150 10 an an DT cord-268467-btfz6ye8 150 11 internal internal JJ cord-268467-btfz6ye8 150 12 Pstl Pstl NNP cord-268467-btfz6ye8 150 13 site site NN cord-268467-btfz6ye8 150 14 toward toward IN cord-268467-btfz6ye8 150 15 the the DT cord-268467-btfz6ye8 150 16 3'-end 3'-end CD cord-268467-btfz6ye8 150 17 was be VBD cord-268467-btfz6ye8 150 18 sequenced sequence VBN cord-268467-btfz6ye8 150 19 . . . cord-268467-btfz6ye8 151 1 Clone Clone NNP cord-268467-btfz6ye8 151 2 L37 L37 NNP cord-268467-btfz6ye8 151 3 was be VBD cord-268467-btfz6ye8 151 4 also also RB cord-268467-btfz6ye8 151 5 sequenced sequence VBN cord-268467-btfz6ye8 151 6 in in IN cord-268467-btfz6ye8 151 7 part part NN cord-268467-btfz6ye8 151 8 . . . cord-268467-btfz6ye8 152 1 Figure figure NN cord-268467-btfz6ye8 152 2 3c 3c CD cord-268467-btfz6ye8 152 3 shows show VBZ cord-268467-btfz6ye8 152 4 the the DT cord-268467-btfz6ye8 152 5 cDNA cdna NN cord-268467-btfz6ye8 152 6 fragments fragment NNS cord-268467-btfz6ye8 152 7 and and CC cord-268467-btfz6ye8 152 8 strategy strategy NN cord-268467-btfz6ye8 152 9 used use VBN cord-268467-btfz6ye8 152 10 in in IN cord-268467-btfz6ye8 152 11 sequencing sequencing NN cord-268467-btfz6ye8 152 12 . . . cord-268467-btfz6ye8 153 1 Each each DT cord-268467-btfz6ye8 153 2 region region NN cord-268467-btfz6ye8 153 3 A a DT cord-268467-btfz6ye8 153 4 primer primer NN cord-268467-btfz6ye8 153 5 extension extension NN cord-268467-btfz6ye8 153 6 study study NN cord-268467-btfz6ye8 153 7 was be VBD cord-268467-btfz6ye8 153 8 carried carry VBN cord-268467-btfz6ye8 153 9 out out RP cord-268467-btfz6ye8 153 10 using use VBG cord-268467-btfz6ye8 153 11 a a DT cord-268467-btfz6ye8 153 12 synthetic synthetic JJ cord-268467-btfz6ye8 153 13 oligodeoxyribonucleotide oligodeoxyribonucleotide NN cord-268467-btfz6ye8 153 14 complementary complementary JJ cord-268467-btfz6ye8 153 15 to to IN cord-268467-btfz6ye8 153 16 an an DT cord-268467-btfz6ye8 153 17 18.mer 18.mer CD cord-268467-btfz6ye8 153 18 sequence sequence NN cord-268467-btfz6ye8 153 19 underlined underline VBN cord-268467-btfz6ye8 153 20 near near IN cord-268467-btfz6ye8 153 21 the the DT cord-268467-btfz6ye8 153 22 5'.end 5'.end CD cord-268467-btfz6ye8 153 23 of of IN cord-268467-btfz6ye8 153 24 the the DT cord-268467-btfz6ye8 153 25 gene gene NN cord-268467-btfz6ye8 153 26 . . . cord-268467-btfz6ye8 154 1 The the DT cord-268467-btfz6ye8 154 2 3'noncoding 3'noncoding CD cord-268467-btfz6ye8 154 3 region region NN cord-268467-btfz6ye8 154 4 contains contain VBZ cord-268467-btfz6ye8 154 5 a a DT cord-268467-btfz6ye8 154 6 conserved conserved JJ cord-268467-btfz6ye8 154 7 sequence sequence NN cord-268467-btfz6ye8 154 8 which which WDT cord-268467-btfz6ye8 154 9 is be VBZ cord-268467-btfz6ye8 154 10 shown show VBN cord-268467-btfz6ye8 154 11 by by IN cord-268467-btfz6ye8 154 12 the the DT cord-268467-btfz6ye8 154 13 double double JJ cord-268467-btfz6ye8 154 14 line line NN cord-268467-btfz6ye8 154 15 . . . cord-268467-btfz6ye8 155 1 The the DT cord-268467-btfz6ye8 155 2 intergenic intergenic JJ cord-268467-btfz6ye8 155 3 conserved conserved JJ cord-268467-btfz6ye8 155 4 sequence sequence NN cord-268467-btfz6ye8 155 5 , , , cord-268467-btfz6ye8 155 6 TCTAAACT TCTAAACT NNP cord-268467-btfz6ye8 155 7 , , , cord-268467-btfz6ye8 155 8 is be VBZ cord-268467-btfz6ye8 155 9 also also RB cord-268467-btfz6ye8 155 10 shown show VBN cord-268467-btfz6ye8 155 11 ( ( -LRB- cord-268467-btfz6ye8 155 12 dotted dotted JJ cord-268467-btfz6ye8 155 13 line line NN cord-268467-btfz6ye8 155 14 ) ) -RRB- cord-268467-btfz6ye8 155 15 was be VBD cord-268467-btfz6ye8 155 16 verified verify VBN cord-268467-btfz6ye8 155 17 by by IN cord-268467-btfz6ye8 155 18 dideoxy dideoxy NN cord-268467-btfz6ye8 155 19 chain chain NN cord-268467-btfz6ye8 155 20 termination termination NN cord-268467-btfz6ye8 155 21 sequencing sequencing NN cord-268467-btfz6ye8 155 22 of of IN cord-268467-btfz6ye8 155 23 both both DT cord-268467-btfz6ye8 155 24 strands strand NNS cord-268467-btfz6ye8 155 25 or or CC cord-268467-btfz6ye8 155 26 by by IN cord-268467-btfz6ye8 155 27 the the DT cord-268467-btfz6ye8 155 28 chemical chemical NN cord-268467-btfz6ye8 155 29 modification modification NN cord-268467-btfz6ye8 155 30 method method NN cord-268467-btfz6ye8 155 31 . . . cord-268467-btfz6ye8 156 1 Clone clone NN cord-268467-btfz6ye8 156 2 Sl Sl NNP cord-268467-btfz6ye8 156 3 0 0 NNP cord-268467-btfz6ye8 156 4 was be VBD cord-268467-btfz6ye8 156 5 found find VBN cord-268467-btfz6ye8 156 6 to to TO cord-268467-btfz6ye8 156 7 have have VB cord-268467-btfz6ye8 156 8 a a DT cord-268467-btfz6ye8 156 9 poly(A poly(a JJ cord-268467-btfz6ye8 156 10 ) ) -RRB- cord-268467-btfz6ye8 156 11 stretch stretch NN cord-268467-btfz6ye8 156 12 of of IN cord-268467-btfz6ye8 156 13 34 34 CD cord-268467-btfz6ye8 156 14 bases basis NNS cord-268467-btfz6ye8 156 15 . . . cord-268467-btfz6ye8 157 1 Figure figure NN cord-268467-btfz6ye8 157 2 4 4 CD cord-268467-btfz6ye8 157 3 shows show VBZ cord-268467-btfz6ye8 157 4 the the DT cord-268467-btfz6ye8 157 5 complete complete JJ cord-268467-btfz6ye8 157 6 DNA dna NN cord-268467-btfz6ye8 157 7 sequence sequence NN cord-268467-btfz6ye8 157 8 with with IN cord-268467-btfz6ye8 157 9 a a DT cord-268467-btfz6ye8 157 10 translation translation NN cord-268467-btfz6ye8 157 11 of of IN cord-268467-btfz6ye8 157 12 the the DT cord-268467-btfz6ye8 157 13 main main JJ cord-268467-btfz6ye8 157 14 open open JJ cord-268467-btfz6ye8 157 15 reading reading NN cord-268467-btfz6ye8 157 16 frame frame NN cord-268467-btfz6ye8 157 17 ( ( -LRB- cord-268467-btfz6ye8 157 18 ORF ORF NNP cord-268467-btfz6ye8 157 19 ) ) -RRB- cord-268467-btfz6ye8 157 20 in in IN cord-268467-btfz6ye8 157 21 one one CD cord-268467-btfz6ye8 157 22 - - HYPH cord-268467-btfz6ye8 157 23 letter letter NN cord-268467-btfz6ye8 157 24 amino amino NN cord-268467-btfz6ye8 157 25 acid acid NN cord-268467-btfz6ye8 157 26 code code NN cord-268467-btfz6ye8 157 27 . . . cord-268467-btfz6ye8 158 1 This this DT cord-268467-btfz6ye8 158 2 ORF ORF NNP cord-268467-btfz6ye8 158 3 extends extend VBZ cord-268467-btfz6ye8 158 4 from from IN cord-268467-btfz6ye8 158 5 base base NN cord-268467-btfz6ye8 158 6 147 147 CD cord-268467-btfz6ye8 158 7 to to TO cord-268467-btfz6ye8 158 8 base base VB cord-268467-btfz6ye8 158 9 13 13 CD cord-268467-btfz6ye8 158 10 13 13 CD cord-268467-btfz6ye8 158 11 and and CC cord-268467-btfz6ye8 158 12 predicts predict VBZ cord-268467-btfz6ye8 158 13 a a DT cord-268467-btfz6ye8 158 14 389 389 CD cord-268467-btfz6ye8 158 15 amino amino NN cord-268467-btfz6ye8 158 16 acid acid NN cord-268467-btfz6ye8 158 17 protein protein NN cord-268467-btfz6ye8 158 18 with with IN cord-268467-btfz6ye8 158 19 a a DT cord-268467-btfz6ye8 158 20 molecular molecular JJ cord-268467-btfz6ye8 158 21 weight weight NN cord-268467-btfz6ye8 158 22 of of IN cord-268467-btfz6ye8 158 23 43,366 43,366 CD cord-268467-btfz6ye8 158 24 Da Da NNS cord-268467-btfz6ye8 158 25 . . . cord-268467-btfz6ye8 159 1 This this DT cord-268467-btfz6ye8 159 2 predicted predict VBD cord-268467-btfz6ye8 159 3 molecular molecular JJ cord-268467-btfz6ye8 159 4 weight weight NN cord-268467-btfz6ye8 159 5 is be VBZ cord-268467-btfz6ye8 159 6 slightly slightly RB cord-268467-btfz6ye8 159 7 smaller small JJR cord-268467-btfz6ye8 159 8 than than IN cord-268467-btfz6ye8 159 9 the the DT cord-268467-btfz6ye8 159 10 measured measure VBN cord-268467-btfz6ye8 159 11 molecular molecular JJ cord-268467-btfz6ye8 159 12 weight weight NN cord-268467-btfz6ye8 159 13 of of IN cord-268467-btfz6ye8 159 14 the the DT cord-268467-btfz6ye8 159 15 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 159 16 protein protein NN cord-268467-btfz6ye8 159 17 of of IN cord-268467-btfz6ye8 159 18 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 159 19 , , , cord-268467-btfz6ye8 159 20 which which WDT cord-268467-btfz6ye8 159 21 is be VBZ cord-268467-btfz6ye8 159 22 50 50 CD cord-268467-btfz6ye8 159 23 kDa kDa NNS cord-268467-btfz6ye8 159 24 as as IN cord-268467-btfz6ye8 159 25 determined determine VBN cord-268467-btfz6ye8 159 26 by by IN cord-268467-btfz6ye8 159 27 SDS SDS NNP cord-268467-btfz6ye8 159 28 - - HYPH cord-268467-btfz6ye8 159 29 polyacrylamide polyacrylamide NN cord-268467-btfz6ye8 159 30 gel gel NN cord-268467-btfz6ye8 159 31 electrophoresis electrophoresis NN cord-268467-btfz6ye8 159 32 ( ( -LRB- cord-268467-btfz6ye8 159 33 MacNaughton MacNaughton NNP cord-268467-btfz6ye8 159 34 , , , cord-268467-btfz6ye8 159 35 1980 1980 CD cord-268467-btfz6ye8 159 36 ) ) -RRB- cord-268467-btfz6ye8 159 37 . . . cord-268467-btfz6ye8 160 1 The the DT cord-268467-btfz6ye8 160 2 difference difference NN cord-268467-btfz6ye8 160 3 is be VBZ cord-268467-btfz6ye8 160 4 probably probably RB cord-268467-btfz6ye8 160 5 due due JJ cord-268467-btfz6ye8 160 6 to to IN cord-268467-btfz6ye8 160 7 phosphorylation phosphorylation NN cord-268467-btfz6ye8 160 8 or or CC cord-268467-btfz6ye8 160 9 other other JJ cord-268467-btfz6ye8 160 10 modification modification NN cord-268467-btfz6ye8 160 11 of of IN cord-268467-btfz6ye8 160 12 the the DT cord-268467-btfz6ye8 160 13 protein protein NN cord-268467-btfz6ye8 160 14 . . . cord-268467-btfz6ye8 161 1 The the DT cord-268467-btfz6ye8 161 2 predicted predict VBN cord-268467-btfz6ye8 161 3 protein protein NN cord-268467-btfz6ye8 161 4 shares share NNS cord-268467-btfz6ye8 161 5 features feature VBZ cord-268467-btfz6ye8 161 6 with with IN cord-268467-btfz6ye8 161 7 the the DT cord-268467-btfz6ye8 161 8 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 161 9 proteins protein NNS cord-268467-btfz6ye8 161 10 of of IN cord-268467-btfz6ye8 161 11 TGEV TGEV NNP cord-268467-btfz6ye8 161 12 , , , cord-268467-btfz6ye8 161 13 MHV MHV NNP cord-268467-btfz6ye8 161 14 , , , cord-268467-btfz6ye8 161 15 BCV BCV NNP cord-268467-btfz6ye8 161 16 , , , cord-268467-btfz6ye8 161 17 HCV HCV NNP cord-268467-btfz6ye8 161 18 - - HYPH cord-268467-btfz6ye8 161 19 OC43 OC43 NNP cord-268467-btfz6ye8 161 20 , , , cord-268467-btfz6ye8 161 21 and and CC cord-268467-btfz6ye8 161 22 IBV IBV NNP cord-268467-btfz6ye8 161 23 ( ( -LRB- cord-268467-btfz6ye8 161 24 Kapke Kapke NNP cord-268467-btfz6ye8 161 25 and and CC cord-268467-btfz6ye8 161 26 Brian Brian NNP cord-268467-btfz6ye8 161 27 , , , cord-268467-btfz6ye8 161 28 1986 1986 CD cord-268467-btfz6ye8 161 29 ; ; : cord-268467-btfz6ye8 161 30 Skinner Skinner NNP cord-268467-btfz6ye8 161 31 and and CC cord-268467-btfz6ye8 161 32 Siddell Siddell NNP cord-268467-btfz6ye8 161 33 , , , cord-268467-btfz6ye8 161 34 1984 1984 CD cord-268467-btfz6ye8 161 35 ; ; . cord-268467-btfz6ye8 162 1 Armstrong Armstrong NNP cord-268467-btfz6ye8 162 2 er er UH cord-268467-btfz6ye8 162 3 a/. a/. NNP cord-268467-btfz6ye8 162 4 , , , cord-268467-btfz6ye8 162 5 1983 1983 CD cord-268467-btfz6ye8 162 6 ; ; : cord-268467-btfz6ye8 163 1 Lapps Lapps NNP cord-268467-btfz6ye8 163 2 et et NNP cord-268467-btfz6ye8 163 3 a/. a/. NN cord-268467-btfz6ye8 163 4 , , , cord-268467-btfz6ye8 163 5 1987 1987 CD cord-268467-btfz6ye8 163 6 ; ; : cord-268467-btfz6ye8 163 7 Kamahora Kamahora NNP cord-268467-btfz6ye8 163 8 et et NNP cord-268467-btfz6ye8 163 9 al al NNP cord-268467-btfz6ye8 163 10 . . NNP cord-268467-btfz6ye8 163 11 , , , cord-268467-btfz6ye8 163 12 1988 1988 CD cord-268467-btfz6ye8 163 13 ; ; . cord-268467-btfz6ye8 164 1 Boursnell Boursnell NNP cord-268467-btfz6ye8 164 2 et et IN cord-268467-btfz6ye8 164 3 a/. a/. NN cord-268467-btfz6ye8 164 4 , , , cord-268467-btfz6ye8 164 5 1985 1985 CD cord-268467-btfz6ye8 164 6 ) ) -RRB- cord-268467-btfz6ye8 164 7 . . . cord-268467-btfz6ye8 165 1 Namely namely RB cord-268467-btfz6ye8 165 2 , , , cord-268467-btfz6ye8 165 3 the the DT cord-268467-btfz6ye8 165 4 protein protein NN cord-268467-btfz6ye8 165 5 is be VBZ cord-268467-btfz6ye8 165 6 highly highly RB cord-268467-btfz6ye8 165 7 basic basic JJ cord-268467-btfz6ye8 165 8 and and CC cord-268467-btfz6ye8 165 9 rich rich JJ cord-268467-btfz6ye8 165 10 in in IN cord-268467-btfz6ye8 165 11 serine serine JJ cord-268467-btfz6ye8 165 12 residues residue NNS cord-268467-btfz6ye8 165 13 . . . cord-268467-btfz6ye8 166 1 Sixty sixty CD cord-268467-btfz6ye8 166 2 percent percent NN cord-268467-btfz6ye8 166 3 of of IN cord-268467-btfz6ye8 166 4 the the DT cord-268467-btfz6ye8 166 5 amino amino NN cord-268467-btfz6ye8 166 6 acid acid NN cord-268467-btfz6ye8 166 7 residues residue NNS cord-268467-btfz6ye8 166 8 are be VBP cord-268467-btfz6ye8 166 9 basic basic JJ cord-268467-btfz6ye8 166 10 and and CC cord-268467-btfz6ye8 166 11 12 12 CD cord-268467-btfz6ye8 166 12 % % NN cord-268467-btfz6ye8 166 13 are be VBP cord-268467-btfz6ye8 166 14 acidic acidic JJ cord-268467-btfz6ye8 166 15 . . . cord-268467-btfz6ye8 167 1 There there EX cord-268467-btfz6ye8 167 2 are be VBP cord-268467-btfz6ye8 167 3 39 39 CD cord-268467-btfz6ye8 167 4 serine serine NN cord-268467-btfz6ye8 167 5 residues residue NNS cord-268467-btfz6ye8 167 6 ( ( -LRB- cord-268467-btfz6ye8 167 7 10 10 CD cord-268467-btfz6ye8 167 8 % % NN cord-268467-btfz6ye8 167 9 of of IN cord-268467-btfz6ye8 167 10 total total NN cord-268467-btfz6ye8 167 11 ) ) -RRB- cord-268467-btfz6ye8 167 12 , , , cord-268467-btfz6ye8 167 13 which which WDT cord-268467-btfz6ye8 167 14 are be VBP cord-268467-btfz6ye8 167 15 presumed presume VBN cord-268467-btfz6ye8 167 16 to to TO cord-268467-btfz6ye8 167 17 be be VB cord-268467-btfz6ye8 167 18 sites site NNS cord-268467-btfz6ye8 167 19 of of IN cord-268467-btfz6ye8 167 20 phosphorylation phosphorylation NN cord-268467-btfz6ye8 168 1 ( ( -LRB- cord-268467-btfz6ye8 168 2 Stohlman Stohlman NNP cord-268467-btfz6ye8 168 3 and and CC cord-268467-btfz6ye8 168 4 Lai Lai NNP cord-268467-btfz6ye8 168 5 , , , cord-268467-btfz6ye8 168 6 1979 1979 CD cord-268467-btfz6ye8 168 7 ) ) -RRB- cord-268467-btfz6ye8 168 8 . . . cord-268467-btfz6ye8 169 1 When when WRB cord-268467-btfz6ye8 169 2 compared compare VBN cord-268467-btfz6ye8 169 3 to to IN cord-268467-btfz6ye8 169 4 TGEV TGEV NNP cord-268467-btfz6ye8 169 5 , , , cord-268467-btfz6ye8 169 6 with with IN cord-268467-btfz6ye8 169 7 which which WDT cord-268467-btfz6ye8 169 8 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 169 9 shares share NNS cord-268467-btfz6ye8 169 10 antigenic antigenic JJ cord-268467-btfz6ye8 169 11 properties property NNS cord-268467-btfz6ye8 169 12 , , , cord-268467-btfz6ye8 169 13 both both CC cord-268467-btfz6ye8 169 14 N n CD cord-268467-btfz6ye8 169 15 proteins protein NNS cord-268467-btfz6ye8 169 16 have have VBP cord-268467-btfz6ye8 169 17 identical identical JJ cord-268467-btfz6ye8 169 18 amounts amount NNS cord-268467-btfz6ye8 169 19 of of IN cord-268467-btfz6ye8 169 20 basic basic JJ cord-268467-btfz6ye8 169 21 and and CC cord-268467-btfz6ye8 169 22 acidic acidic JJ cord-268467-btfz6ye8 169 23 amino amino NN cord-268467-btfz6ye8 169 24 acids acid NNS cord-268467-btfz6ye8 169 25 and and CC cord-268467-btfz6ye8 169 26 serine serine NN cord-268467-btfz6ye8 169 27 residues residue NNS cord-268467-btfz6ye8 169 28 and and CC cord-268467-btfz6ye8 169 29 similar similar JJ cord-268467-btfz6ye8 169 30 molecular molecular JJ cord-268467-btfz6ye8 169 31 weights weight NNS cord-268467-btfz6ye8 169 32 ( ( -LRB- cord-268467-btfz6ye8 169 33 Kapke Kapke NNP cord-268467-btfz6ye8 169 34 and and CC cord-268467-btfz6ye8 169 35 Brian Brian NNP cord-268467-btfz6ye8 169 36 , , , cord-268467-btfz6ye8 169 37 1986 1986 CD cord-268467-btfz6ye8 169 38 ) ) -RRB- cord-268467-btfz6ye8 169 39 . . . cord-268467-btfz6ye8 170 1 Figure figure NN cord-268467-btfz6ye8 170 2 5 5 CD cord-268467-btfz6ye8 170 3 shows show VBZ cord-268467-btfz6ye8 170 4 a a DT cord-268467-btfz6ye8 170 5 schematic schematic JJ cord-268467-btfz6ye8 170 6 diagram diagram NN cord-268467-btfz6ye8 170 7 of of IN cord-268467-btfz6ye8 170 8 the the DT cord-268467-btfz6ye8 170 9 possible possible JJ cord-268467-btfz6ye8 170 10 ORFs orfs NN cord-268467-btfz6ye8 170 11 obtained obtain VBN cord-268467-btfz6ye8 170 12 by by IN cord-268467-btfz6ye8 170 13 translating translate VBG cord-268467-btfz6ye8 170 14 the the DT cord-268467-btfz6ye8 170 15 nucleotide nucleotide JJ cord-268467-btfz6ye8 170 16 sequence sequence NN cord-268467-btfz6ye8 170 17 . . . cord-268467-btfz6ye8 171 1 The the DT cord-268467-btfz6ye8 171 2 ORF ORF NNP cord-268467-btfz6ye8 171 3 in in IN cord-268467-btfz6ye8 171 4 frame frame NN cord-268467-btfz6ye8 171 5 3 3 CD cord-268467-btfz6ye8 171 6 is be VBZ cord-268467-btfz6ye8 171 7 likely likely JJ cord-268467-btfz6ye8 171 8 the the DT cord-268467-btfz6ye8 171 9 one one NN cord-268467-btfz6ye8 171 10 which which WDT cord-268467-btfz6ye8 171 11 encodes encode VBZ cord-268467-btfz6ye8 171 12 the the DT cord-268467-btfz6ye8 171 13 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 171 14 protein protein NN cord-268467-btfz6ye8 171 15 . . . cord-268467-btfz6ye8 172 1 In in IN cord-268467-btfz6ye8 172 2 frame frame NN cord-268467-btfz6ye8 172 3 2 2 CD cord-268467-btfz6ye8 172 4 , , , cord-268467-btfz6ye8 172 5 the the DT cord-268467-btfz6ye8 172 6 5'-flanking 5'-flanking CD cord-268467-btfz6ye8 172 7 region region NN cord-268467-btfz6ye8 172 8 probably probably RB cord-268467-btfz6ye8 172 9 contains contain VBZ cord-268467-btfz6ye8 172 10 part part NN cord-268467-btfz6ye8 172 11 of of IN cord-268467-btfz6ye8 172 12 the the DT cord-268467-btfz6ye8 172 13 sequence sequence NN cord-268467-btfz6ye8 172 14 of of IN cord-268467-btfz6ye8 172 15 the the DT cord-268467-btfz6ye8 172 16 matrix matrix NN cord-268467-btfz6ye8 172 17 protein protein NN cord-268467-btfz6ye8 172 18 encoded encode VBN cord-268467-btfz6ye8 172 19 by by IN cord-268467-btfz6ye8 172 20 gene gene NN cord-268467-btfz6ye8 172 21 6 6 CD cord-268467-btfz6ye8 172 22 . . . cord-268467-btfz6ye8 173 1 This this DT cord-268467-btfz6ye8 173 2 possibility possibility NN cord-268467-btfz6ye8 173 3 is be VBZ cord-268467-btfz6ye8 173 4 sup- sup- JJ cord-268467-btfz6ye8 174 1 --I1 --I1 : cord-268467-btfz6ye8 175 1 I -PRON- PRP cord-268467-btfz6ye8 176 1 II II NNP cord-268467-btfz6ye8 177 1 I -PRON- PRP cord-268467-btfz6ye8 177 2 III iii CD cord-268467-btfz6ye8 177 3 _SP cord-268467-btfz6ye8 178 1 ----- ----- : cord-268467-btfz6ye8 179 1 _ _ NNP cord-268467-btfz6ye8 179 2 _ _ NNP cord-268467-btfz6ye8 179 3 _SP cord-268467-btfz6ye8 180 1 1 1 LS cord-268467-btfz6ye8 180 2 _SP cord-268467-btfz6ye8 181 1 III1 III1 NNP cord-268467-btfz6ye8 181 2 _SP cord-268467-btfz6ye8 182 1 -I -I NFP cord-268467-btfz6ye8 182 2 _SP cord-268467-btfz6ye8 183 1 -llil -llil NNP cord-268467-btfz6ye8 183 2 _SP cord-268467-btfz6ye8 184 1 I -PRON- PRP cord-268467-btfz6ye8 184 2 _SP cord-268467-btfz6ye8 184 3 II II NNP cord-268467-btfz6ye8 184 4 _SP cord-268467-btfz6ye8 185 1 II II NNP cord-268467-btfz6ye8 185 2 _SP cord-268467-btfz6ye8 186 1 I -PRON- PRP cord-268467-btfz6ye8 187 1 I -PRON- PRP cord-268467-btfz6ye8 187 2 _SP cord-268467-btfz6ye8 188 1 I -PRON- PRP cord-268467-btfz6ye8 188 2 _SP cord-268467-btfz6ye8 189 1 III III NNP cord-268467-btfz6ye8 190 1 I -PRON- PRP cord-268467-btfz6ye8 190 2 II ii CD cord-268467-btfz6ye8 190 3 _SP cord-268467-btfz6ye8 191 1 2-J 2-j CD cord-268467-btfz6ye8 191 2 _SP cord-268467-btfz6ye8 192 1 I -PRON- PRP cord-268467-btfz6ye8 193 1 I -PRON- PRP cord-268467-btfz6ye8 194 1 111111 111111 CD cord-268467-btfz6ye8 194 2 II II NNP cord-268467-btfz6ye8 194 3 I1111111 I1111111 NNP cord-268467-btfz6ye8 194 4 lllll lllll NNP cord-268467-btfz6ye8 194 5 _SP cord-268467-btfz6ye8 195 1 III III NNP cord-268467-btfz6ye8 195 2 I -PRON- PRP cord-268467-btfz6ye8 195 3 II II NNP cord-268467-btfz6ye8 196 1 , , , cord-268467-btfz6ye8 196 2 1111 1111 CD cord-268467-btfz6ye8 196 3 I -PRON- PRP cord-268467-btfz6ye8 197 1 Ill1 Ill1 NNP cord-268467-btfz6ye8 197 2 IIII IIII NNP cord-268467-btfz6ye8 198 1 I -PRON- PRP cord-268467-btfz6ye8 198 2 _SP cord-268467-btfz6ye8 199 1 3 3 CD cord-268467-btfz6ye8 199 2 _SP cord-268467-btfz6ye8 200 1 I -PRON- PRP cord-268467-btfz6ye8 200 2 _SP cord-268467-btfz6ye8 201 1 I -PRON- PRP cord-268467-btfz6ye8 201 2 _SP cord-268467-btfz6ye8 202 1 I -PRON- PRP cord-268467-btfz6ye8 202 2 _SP cord-268467-btfz6ye8 203 1 I -PRON- PRP cord-268467-btfz6ye8 203 2 _SP cord-268467-btfz6ye8 204 1 Ill Ill NNP cord-268467-btfz6ye8 204 2 _SP cord-268467-btfz6ye8 205 1 I -PRON- PRP cord-268467-btfz6ye8 206 1 I -PRON- PRP cord-268467-btfz6ye8 207 1 I11 I11 NNP cord-268467-btfz6ye8 207 2 _SP cord-268467-btfz6ye8 208 1 I -PRON- PRP cord-268467-btfz6ye8 208 2 _SP cord-268467-btfz6ye8 209 1 I -PRON- PRP cord-268467-btfz6ye8 209 2 _SP cord-268467-btfz6ye8 210 1 I -PRON- PRP cord-268467-btfz6ye8 210 2 _SP cord-268467-btfz6ye8 211 1 I -PRON- PRP cord-268467-btfz6ye8 211 2 _SP cord-268467-btfz6ye8 212 1 I -PRON- PRP cord-268467-btfz6ye8 212 2 ported port VBD cord-268467-btfz6ye8 212 3 by by IN cord-268467-btfz6ye8 212 4 the the DT cord-268467-btfz6ye8 212 5 finding finding NN cord-268467-btfz6ye8 212 6 that that IN cord-268467-btfz6ye8 212 7 reading read VBG cord-268467-btfz6ye8 212 8 frame frame NN cord-268467-btfz6ye8 212 9 2 2 CD cord-268467-btfz6ye8 212 10 remains remain VBZ cord-268467-btfz6ye8 212 11 open open JJ cord-268467-btfz6ye8 212 12 at at IN cord-268467-btfz6ye8 212 13 the the DT cord-268467-btfz6ye8 212 14 extreme extreme JJ cord-268467-btfz6ye8 212 15 5'-end 5'-end CD cord-268467-btfz6ye8 212 16 . . . cord-268467-btfz6ye8 213 1 Furthermore furthermore RB cord-268467-btfz6ye8 213 2 , , , cord-268467-btfz6ye8 213 3 the the DT cord-268467-btfz6ye8 213 4 sequence sequence NN cord-268467-btfz6ye8 213 5 TCTAAACT TCTAAACT NNP cord-268467-btfz6ye8 213 6 , , , cord-268467-btfz6ye8 213 7 which which WDT cord-268467-btfz6ye8 213 8 is be VBZ cord-268467-btfz6ye8 213 9 found find VBN cord-268467-btfz6ye8 213 10 in in IN cord-268467-btfz6ye8 213 11 the the DT cord-268467-btfz6ye8 213 12 intergenic intergenic JJ cord-268467-btfz6ye8 213 13 regions region NNS cord-268467-btfz6ye8 213 14 of of IN cord-268467-btfz6ye8 213 15 several several JJ cord-268467-btfz6ye8 213 16 other other JJ cord-268467-btfz6ye8 213 17 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 213 18 ( ( -LRB- cord-268467-btfz6ye8 213 19 Kapke Kapke NNP cord-268467-btfz6ye8 213 20 and and CC cord-268467-btfz6ye8 213 21 Brian Brian NNP cord-268467-btfz6ye8 213 22 , , , cord-268467-btfz6ye8 213 23 1986 1986 CD cord-268467-btfz6ye8 213 24 ; ; : cord-268467-btfz6ye8 213 25 Skinner Skinner NNP cord-268467-btfz6ye8 213 26 and and CC cord-268467-btfz6ye8 213 27 Siddell Siddell NNP cord-268467-btfz6ye8 213 28 , , , cord-268467-btfz6ye8 213 29 1984 1984 CD cord-268467-btfz6ye8 213 30 ; ; : cord-268467-btfz6ye8 213 31 Armstrong Armstrong NNP cord-268467-btfz6ye8 213 32 et et NNP cord-268467-btfz6ye8 213 33 a/. a/. NNP cord-268467-btfz6ye8 213 34 , , , cord-268467-btfz6ye8 213 35 1983 1983 CD cord-268467-btfz6ye8 213 36 ; ; : cord-268467-btfz6ye8 213 37 Lapps Lapps NNP cord-268467-btfz6ye8 213 38 et et NNP cord-268467-btfz6ye8 213 39 al al NNP cord-268467-btfz6ye8 213 40 . . NNP cord-268467-btfz6ye8 213 41 , , , cord-268467-btfz6ye8 213 42 1987 1987 CD cord-268467-btfz6ye8 213 43 ; ; : cord-268467-btfz6ye8 213 44 Kamahora Kamahora NNP cord-268467-btfz6ye8 213 45 et et NNP cord-268467-btfz6ye8 213 46 a/. a/. NNP cord-268467-btfz6ye8 213 47 , , , cord-268467-btfz6ye8 213 48 1988 1988 CD cord-268467-btfz6ye8 213 49 ; ; : cord-268467-btfz6ye8 213 50 Budzilowicz Budzilowicz NNP cord-268467-btfz6ye8 213 51 eta/. eta/. CD cord-268467-btfz6ye8 213 52 , , , cord-268467-btfz6ye8 213 53 1985 1985 CD cord-268467-btfz6ye8 213 54 ) ) -RRB- cord-268467-btfz6ye8 213 55 is be VBZ cord-268467-btfz6ye8 213 56 also also RB cord-268467-btfz6ye8 213 57 present present JJ cord-268467-btfz6ye8 213 58 between between IN cord-268467-btfz6ye8 213 59 the the DT cord-268467-btfz6ye8 213 60 presumed presume VBN cord-268467-btfz6ye8 213 61 initiation initiation NN cord-268467-btfz6ye8 213 62 codon codon NN cord-268467-btfz6ye8 213 63 of of IN cord-268467-btfz6ye8 213 64 the the DT cord-268467-btfz6ye8 213 65 main main JJ cord-268467-btfz6ye8 213 66 ORF ORF NNP cord-268467-btfz6ye8 213 67 and and CC cord-268467-btfz6ye8 213 68 the the DT cord-268467-btfz6ye8 213 69 3'-end 3'-end CD cord-268467-btfz6ye8 213 70 of of IN cord-268467-btfz6ye8 213 71 gene gene NN cord-268467-btfz6ye8 213 72 6 6 CD cord-268467-btfz6ye8 213 73 . . . cord-268467-btfz6ye8 214 1 This this DT cord-268467-btfz6ye8 214 2 sequence sequence NN cord-268467-btfz6ye8 214 3 is be VBZ cord-268467-btfz6ye8 214 4 the the DT cord-268467-btfz6ye8 214 5 proposed propose VBN cord-268467-btfz6ye8 214 6 site site NN cord-268467-btfz6ye8 214 7 of of IN cord-268467-btfz6ye8 214 8 fusion fusion NN cord-268467-btfz6ye8 214 9 of of IN cord-268467-btfz6ye8 214 10 the the DT cord-268467-btfz6ye8 214 11 leader leader NN cord-268467-btfz6ye8 214 12 sequence sequence NN cord-268467-btfz6ye8 214 13 with with IN cord-268467-btfz6ye8 214 14 the the DT cord-268467-btfz6ye8 214 15 mRNA mrna NN cord-268467-btfz6ye8 214 16 coding code VBG cord-268467-btfz6ye8 214 17 region region NN cord-268467-btfz6ye8 214 18 Makino Makino NNP cord-268467-btfz6ye8 214 19 et et NNP cord-268467-btfz6ye8 214 20 al al NNP cord-268467-btfz6ye8 214 21 . . . cord-268467-btfz6ye8 215 1 , , , cord-268467-btfz6ye8 215 2 1986 1986 CD cord-268467-btfz6ye8 215 3 ; ; : cord-268467-btfz6ye8 215 4 Budzilowicz Budzilowicz NNP cord-268467-btfz6ye8 215 5 eT eT NNP cord-268467-btfz6ye8 216 1 al al NNP cord-268467-btfz6ye8 216 2 . . NNP cord-268467-btfz6ye8 216 3 , , , cord-268467-btfz6ye8 216 4 1985 1985 CD cord-268467-btfz6ye8 216 5 ) ) -RRB- cord-268467-btfz6ye8 216 6 . . . cord-268467-btfz6ye8 217 1 The the DT cord-268467-btfz6ye8 217 2 3'-noncoding 3'-noncode VBG cord-268467-btfz6ye8 217 3 region region NN cord-268467-btfz6ye8 217 4 contains contain VBZ cord-268467-btfz6ye8 217 5 the the DT cord-268467-btfz6ye8 217 6 sequence sequence NN cord-268467-btfz6ye8 217 7 TGGAAGAGCCA TGGAAGAGCCA NNP cord-268467-btfz6ye8 217 8 , , , cord-268467-btfz6ye8 217 9 75 75 CD cord-268467-btfz6ye8 217 10 nucleotides nucleotide NNS cord-268467-btfz6ye8 217 11 from from IN cord-268467-btfz6ye8 217 12 the the DT cord-268467-btfz6ye8 217 13 3'-end 3'-end CD cord-268467-btfz6ye8 218 1 ( ( -LRB- cord-268467-btfz6ye8 218 2 Fig Fig NNP cord-268467-btfz6ye8 218 3 . . . cord-268467-btfz6ye8 218 4 _SP cord-268467-btfz6ye8 218 5 4 4 CD cord-268467-btfz6ye8 218 6 ) ) -RRB- cord-268467-btfz6ye8 218 7 which which WDT cord-268467-btfz6ye8 218 8 is be VBZ cord-268467-btfz6ye8 218 9 relatively relatively RB cord-268467-btfz6ye8 218 10 conserved conserved JJ cord-268467-btfz6ye8 218 11 among among IN cord-268467-btfz6ye8 218 12 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 218 13 and and CC cord-268467-btfz6ye8 218 14 is be VBZ cord-268467-btfz6ye8 218 15 found find VBN cord-268467-btfz6ye8 218 16 at at IN cord-268467-btfz6ye8 218 17 approximately approximately RB cord-268467-btfz6ye8 218 18 the the DT cord-268467-btfz6ye8 218 19 same same JJ cord-268467-btfz6ye8 218 20 location location NN cord-268467-btfz6ye8 218 21 in in IN cord-268467-btfz6ye8 218 22 all all DT cord-268467-btfz6ye8 218 23 of of IN cord-268467-btfz6ye8 218 24 these these DT cord-268467-btfz6ye8 218 25 viral viral JJ cord-268467-btfz6ye8 218 26 genomes genome NNS cord-268467-btfz6ye8 218 27 ( ( -LRB- cord-268467-btfz6ye8 218 28 Kapke Kapke NNP cord-268467-btfz6ye8 218 29 and and CC cord-268467-btfz6ye8 218 30 Brian Brian NNP cord-268467-btfz6ye8 218 31 , , , cord-268467-btfz6ye8 218 32 1986 1986 CD cord-268467-btfz6ye8 218 33 ; ; : cord-268467-btfz6ye8 218 34 Skinner Skinner NNP cord-268467-btfz6ye8 218 35 and and CC cord-268467-btfz6ye8 218 36 Siddell Siddell NNP cord-268467-btfz6ye8 218 37 , , , cord-268467-btfz6ye8 218 38 1984 1984 CD cord-268467-btfz6ye8 218 39 ; ; : cord-268467-btfz6ye8 218 40 Armstrong Armstrong NNP cord-268467-btfz6ye8 218 41 et et NNP cord-268467-btfz6ye8 218 42 a/. a/. NNP cord-268467-btfz6ye8 218 43 , , , cord-268467-btfz6ye8 218 44 1983 1983 CD cord-268467-btfz6ye8 218 45 ; ; : cord-268467-btfz6ye8 218 46 Lapps Lapps NNP cord-268467-btfz6ye8 218 47 et et NNP cord-268467-btfz6ye8 218 48 al al NNP cord-268467-btfz6ye8 218 49 . . NNP cord-268467-btfz6ye8 218 50 , , , cord-268467-btfz6ye8 218 51 1987 1987 CD cord-268467-btfz6ye8 218 52 ; ; : cord-268467-btfz6ye8 218 53 Kamahora Kamahora NNP cord-268467-btfz6ye8 218 54 et et NNP cord-268467-btfz6ye8 218 55 a/. a/. NNP cord-268467-btfz6ye8 218 56 , , , cord-268467-btfz6ye8 218 57 1988 1988 CD cord-268467-btfz6ye8 218 58 ; ; : cord-268467-btfz6ye8 219 1 Boursnell Boursnell NNP cord-268467-btfz6ye8 219 2 et et FW cord-268467-btfz6ye8 219 3 al al NNP cord-268467-btfz6ye8 219 4 . . NNP cord-268467-btfz6ye8 219 5 , , , cord-268467-btfz6ye8 219 6 1985 1985 CD cord-268467-btfz6ye8 219 7 ) ) -RRB- cord-268467-btfz6ye8 220 1 ( ( -LRB- cord-268467-btfz6ye8 220 2 Table table NN cord-268467-btfz6ye8 220 3 1 1 CD cord-268467-btfz6ye8 220 4 ) ) -RRB- cord-268467-btfz6ye8 220 5 . . . cord-268467-btfz6ye8 221 1 There there EX cord-268467-btfz6ye8 221 2 is be VBZ cord-268467-btfz6ye8 221 3 only only RB cord-268467-btfz6ye8 221 4 one one CD cord-268467-btfz6ye8 221 5 nucleotide nucleotide JJ cord-268467-btfz6ye8 221 6 difference difference NN cord-268467-btfz6ye8 221 7 in in IN cord-268467-btfz6ye8 221 8 this this DT cord-268467-btfz6ye8 221 9 conserved conserve VBN cord-268467-btfz6ye8 221 10 sequence sequence NN cord-268467-btfz6ye8 221 11 when when WRB cord-268467-btfz6ye8 221 12 it -PRON- PRP cord-268467-btfz6ye8 221 13 is be VBZ cord-268467-btfz6ye8 221 14 compared compare VBN cord-268467-btfz6ye8 221 15 with with IN cord-268467-btfz6ye8 221 16 that that DT cord-268467-btfz6ye8 221 17 of of IN cord-268467-btfz6ye8 221 18 TGEV TGEV NNP cord-268467-btfz6ye8 221 19 , , , cord-268467-btfz6ye8 221 20 BCV BCV NNP cord-268467-btfz6ye8 221 21 , , , cord-268467-btfz6ye8 221 22 and and CC cord-268467-btfz6ye8 221 23 HCV HCV NNP cord-268467-btfz6ye8 221 24 - - HYPH cord-268467-btfz6ye8 221 25 OC43 OC43 NNP cord-268467-btfz6ye8 221 26 . . . cord-268467-btfz6ye8 222 1 Two two CD cord-268467-btfz6ye8 222 2 and and CC cord-268467-btfz6ye8 222 3 three three CD cord-268467-btfz6ye8 222 4 nucleotide nucleotide JJ cord-268467-btfz6ye8 222 5 differences difference NNS cord-268467-btfz6ye8 222 6 are be VBP cord-268467-btfz6ye8 222 7 found find VBN cord-268467-btfz6ye8 222 8 in in IN cord-268467-btfz6ye8 222 9 IBV IBV NNP cord-268467-btfz6ye8 222 10 and and CC cord-268467-btfz6ye8 222 11 MHV MHV NNP cord-268467-btfz6ye8 222 12 , , , cord-268467-btfz6ye8 222 13 respectively respectively RB cord-268467-btfz6ye8 222 14 . . . cord-268467-btfz6ye8 223 1 This this DT cord-268467-btfz6ye8 223 2 conservation conservation NN cord-268467-btfz6ye8 223 3 of of IN cord-268467-btfz6ye8 223 4 sequence sequence NN cord-268467-btfz6ye8 223 5 and and CC cord-268467-btfz6ye8 223 6 location location NN cord-268467-btfz6ye8 223 7 suggests suggest VBZ cord-268467-btfz6ye8 223 8 that that IN cord-268467-btfz6ye8 223 9 it -PRON- PRP cord-268467-btfz6ye8 223 10 may may MD cord-268467-btfz6ye8 223 11 be be VB cord-268467-btfz6ye8 223 12 important important JJ cord-268467-btfz6ye8 223 13 for for IN cord-268467-btfz6ye8 223 14 viral viral JJ cord-268467-btfz6ye8 223 15 RNA RNA NNP cord-268467-btfz6ye8 223 16 replication replication NN cord-268467-btfz6ye8 223 17 . . . cord-268467-btfz6ye8 224 1 In in IN cord-268467-btfz6ye8 224 2 frame frame NN cord-268467-btfz6ye8 224 3 1 1 CD cord-268467-btfz6ye8 224 4 , , , cord-268467-btfz6ye8 224 5 there there EX cord-268467-btfz6ye8 224 6 are be VBP cord-268467-btfz6ye8 224 7 several several JJ cord-268467-btfz6ye8 224 8 additional additional JJ cord-268467-btfz6ye8 224 9 ORFs orf NNS cord-268467-btfz6ye8 224 10 of of IN cord-268467-btfz6ye8 224 11 at at RB cord-268467-btfz6ye8 224 12 least least RBS cord-268467-btfz6ye8 224 13 30 30 CD cord-268467-btfz6ye8 224 14 amino amino NN cord-268467-btfz6ye8 224 15 acids acid NNS cord-268467-btfz6ye8 224 16 . . . cord-268467-btfz6ye8 225 1 Some some DT cord-268467-btfz6ye8 225 2 of of IN cord-268467-btfz6ye8 225 3 these these DT cord-268467-btfz6ye8 225 4 , , , cord-268467-btfz6ye8 225 5 including include VBG cord-268467-btfz6ye8 225 6 one one CD cord-268467-btfz6ye8 225 7 found find VBN cord-268467-btfz6ye8 225 8 in in IN cord-268467-btfz6ye8 225 9 the the DT cord-268467-btfz6ye8 225 10 3'-noncoding 3'-noncoding NN cord-268467-btfz6ye8 225 11 region region NN cord-268467-btfz6ye8 225 12 , , , cord-268467-btfz6ye8 225 13 lack lack VBP cord-268467-btfz6ye8 225 14 appropriate appropriate JJ cord-268467-btfz6ye8 225 15 translation translation NN cord-268467-btfz6ye8 225 16 start start NN cord-268467-btfz6ye8 225 17 sites site NNS cord-268467-btfz6ye8 225 18 . . . cord-268467-btfz6ye8 226 1 Another another DT cord-268467-btfz6ye8 226 2 long long JJ cord-268467-btfz6ye8 226 3 internal internal JJ cord-268467-btfz6ye8 226 4 ORF ORF NNP cord-268467-btfz6ye8 226 5 is be VBZ cord-268467-btfz6ye8 226 6 found find VBN cord-268467-btfz6ye8 226 7 from from IN cord-268467-btfz6ye8 226 8 base base NN cord-268467-btfz6ye8 226 9 322 322 CD cord-268467-btfz6ye8 226 10 through through IN cord-268467-btfz6ye8 226 11 693 693 CD cord-268467-btfz6ye8 226 12 . . . cord-268467-btfz6ye8 227 1 This this DT cord-268467-btfz6ye8 227 2 contains contain VBZ cord-268467-btfz6ye8 227 3 an an DT cord-268467-btfz6ye8 227 4 appropriate appropriate JJ cord-268467-btfz6ye8 227 5 initiation initiation NN cord-268467-btfz6ye8 227 6 sequence sequence NN cord-268467-btfz6ye8 227 7 and and CC cord-268467-btfz6ye8 227 8 encodes encode VBZ cord-268467-btfz6ye8 227 9 a a DT cord-268467-btfz6ye8 227 10 hypothetical hypothetical JJ cord-268467-btfz6ye8 227 11 protein protein NN cord-268467-btfz6ye8 227 12 of of IN cord-268467-btfz6ye8 227 13 13,974 13,974 CD cord-268467-btfz6ye8 227 14 Da Da NNP cord-268467-btfz6ye8 227 15 , , , cord-268467-btfz6ye8 227 16 which which WDT cord-268467-btfz6ye8 227 17 is be VBZ cord-268467-btfz6ye8 227 18 rich rich JJ cord-268467-btfz6ye8 227 19 in in IN cord-268467-btfz6ye8 227 20 leucine leucine NN cord-268467-btfz6ye8 227 21 residues residue NNS cord-268467-btfz6ye8 227 22 ( ( -LRB- cord-268467-btfz6ye8 227 23 17 17 CD cord-268467-btfz6ye8 227 24 % % NN cord-268467-btfz6ye8 227 25 ) ) -RRB- cord-268467-btfz6ye8 227 26 . . . cord-268467-btfz6ye8 228 1 The the DT cord-268467-btfz6ye8 228 2 significance significance NN cord-268467-btfz6ye8 228 3 of of IN cord-268467-btfz6ye8 228 4 this this DT cord-268467-btfz6ye8 228 5 ORF ORF NNP cord-268467-btfz6ye8 228 6 remains remain VBZ cord-268467-btfz6ye8 228 7 to to TO cord-268467-btfz6ye8 228 8 be be VB cord-268467-btfz6ye8 228 9 defined define VBN cord-268467-btfz6ye8 228 10 . . . cord-268467-btfz6ye8 229 1 The the DT cord-268467-btfz6ye8 229 2 mRNAs mrna NNS cord-268467-btfz6ye8 229 3 of of IN cord-268467-btfz6ye8 229 4 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 229 5 contain contain VBP cord-268467-btfz6ye8 229 6 a a DT cord-268467-btfz6ye8 229 7 stretch stretch NN cord-268467-btfz6ye8 229 8 of of IN cord-268467-btfz6ye8 229 9 leader leader NN cord-268467-btfz6ye8 229 10 sequence sequence NN cord-268467-btfz6ye8 229 11 which which WDT cord-268467-btfz6ye8 229 12 is be VBZ cord-268467-btfz6ye8 229 13 derived derive VBN cord-268467-btfz6ye8 229 14 from from IN cord-268467-btfz6ye8 229 15 the the DT cord-268467-btfz6ye8 229 16 5'-end 5'-end CD cord-268467-btfz6ye8 229 17 of of IN cord-268467-btfz6ye8 229 18 the the DT cord-268467-btfz6ye8 229 19 viral viral JJ cord-268467-btfz6ye8 229 20 genome genome NN cord-268467-btfz6ye8 229 21 and and CC cord-268467-btfz6ye8 230 1 exhibits exhibit NNS cord-268467-btfz6ye8 230 2 homologywith homologywith NNP cord-268467-btfz6ye8 230 3 the the DT cord-268467-btfz6ye8 230 4 intergenic intergenic JJ cord-268467-btfz6ye8 230 5 consensus consensus NN cord-268467-btfz6ye8 230 6 sequence sequence NN cord-268467-btfz6ye8 231 1 Budzilowicz Budzilowicz NNP cord-268467-btfz6ye8 231 2 et et NNP cord-268467-btfz6ye8 231 3 al al NNP cord-268467-btfz6ye8 231 4 . . NNP cord-268467-btfz6ye8 231 5 , , , cord-268467-btfz6ye8 231 6 1985 1985 CD cord-268467-btfz6ye8 231 7 ) ) -RRB- cord-268467-btfz6ye8 231 8 . . . cord-268467-btfz6ye8 232 1 Since since IN cord-268467-btfz6ye8 232 2 our -PRON- PRP$ cord-268467-btfz6ye8 232 3 cDNA cdna NN cord-268467-btfz6ye8 232 4 clones clone NNS cord-268467-btfz6ye8 232 5 did do VBD cord-268467-btfz6ye8 232 6 not not RB cord-268467-btfz6ye8 232 7 appear appear VB cord-268467-btfz6ye8 232 8 to to TO cord-268467-btfz6ye8 232 9 contain contain VB cord-268467-btfz6ye8 232 10 leader leader NN cord-268467-btfz6ye8 232 11 sequences sequence NNS cord-268467-btfz6ye8 232 12 , , , cord-268467-btfz6ye8 232 13 we -PRON- PRP cord-268467-btfz6ye8 232 14 used use VBD cord-268467-btfz6ye8 232 15 primer primer NN cord-268467-btfz6ye8 232 16 extension extension NN cord-268467-btfz6ye8 232 17 studies study NNS cord-268467-btfz6ye8 232 18 to to TO cord-268467-btfz6ye8 232 19 determine determine VB cord-268467-btfz6ye8 232 20 the the DT cord-268467-btfz6ye8 232 21 sequence sequence NN cord-268467-btfz6ye8 232 22 of of IN cord-268467-btfz6ye8 232 23 the the DT cord-268467-btfz6ye8 232 24 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 232 25 leader leader NN cord-268467-btfz6ye8 232 26 RNA RNA NNP cord-268467-btfz6ye8 232 27 . . . cord-268467-btfz6ye8 233 1 A a DT cord-268467-btfz6ye8 233 2 synthetic synthetic JJ cord-268467-btfz6ye8 233 3 oligodeoxyribonucleotide oligodeoxyribonucleotide NN cord-268467-btfz6ye8 233 4 which which WDT cord-268467-btfz6ye8 233 5 was be VBD cord-268467-btfz6ye8 233 6 complementary complementary JJ cord-268467-btfz6ye8 233 7 to to IN cord-268467-btfz6ye8 233 8 an an DT cord-268467-btfz6ye8 233 9 18-mer 18-mer CD cord-268467-btfz6ye8 233 10 sequence sequence NN cord-268467-btfz6ye8 233 11 located locate VBN cord-268467-btfz6ye8 233 12 near near IN cord-268467-btfz6ye8 233 13 the the DT cord-268467-btfz6ye8 233 14 5'-end 5'-end CD cord-268467-btfz6ye8 233 15 of of IN cord-268467-btfz6ye8 233 16 the the DT cord-268467-btfz6ye8 233 17 gene gene NN cord-268467-btfz6ye8 233 18 ( ( -LRB- cord-268467-btfz6ye8 233 19 Fig Fig NNP cord-268467-btfz6ye8 233 20 . . . cord-268467-btfz6ye8 233 21 4 4 LS cord-268467-btfz6ye8 233 22 ) ) -RRB- cord-268467-btfz6ye8 233 23 was be VBD cord-268467-btfz6ye8 233 24 end end NN cord-268467-btfz6ye8 233 25 - - HYPH cord-268467-btfz6ye8 233 26 labeled label VBN cord-268467-btfz6ye8 233 27 and and CC cord-268467-btfz6ye8 233 28 used use VBN cord-268467-btfz6ye8 233 29 in in IN cord-268467-btfz6ye8 233 30 a a DT cord-268467-btfz6ye8 233 31 primer primer NN cord-268467-btfz6ye8 233 32 extension extension NN cord-268467-btfz6ye8 233 33 study study NN cord-268467-btfz6ye8 233 34 with with IN cord-268467-btfz6ye8 233 35 poly(A)-selected poly(A)-selected NNP cord-268467-btfz6ye8 233 36 intracellular intracellular JJ cord-268467-btfz6ye8 233 37 mRNA mRNA NNP cord-268467-btfz6ye8 233 38 as as IN cord-268467-btfz6ye8 233 39 a a DT cord-268467-btfz6ye8 233 40 template template NN cord-268467-btfz6ye8 233 41 . . . cord-268467-btfz6ye8 234 1 The the DT cord-268467-btfz6ye8 234 2 reaction reaction NN cord-268467-btfz6ye8 234 3 products product NNS cord-268467-btfz6ye8 234 4 , , , cord-268467-btfz6ye8 234 5 separated separate VBN cord-268467-btfz6ye8 234 6 by by IN cord-268467-btfz6ye8 234 7 agarose agarose JJ cord-268467-btfz6ye8 234 8 gel gel NN cord-268467-btfz6ye8 234 9 electrophoresis electrophoresis NN cord-268467-btfz6ye8 234 10 , , , cord-268467-btfz6ye8 234 11 revealed reveal VBD cord-268467-btfz6ye8 234 12 six six CD cord-268467-btfz6ye8 234 13 bands band NNS cord-268467-btfz6ye8 234 14 ( ( -LRB- cord-268467-btfz6ye8 234 15 data datum NNS cord-268467-btfz6ye8 234 16 not not RB cord-268467-btfz6ye8 234 17 shown show VBN cord-268467-btfz6ye8 234 18 ) ) -RRB- cord-268467-btfz6ye8 234 19 . . . cord-268467-btfz6ye8 235 1 Since since IN cord-268467-btfz6ye8 235 2 these these DT cord-268467-btfz6ye8 235 3 bands band NNS cord-268467-btfz6ye8 235 4 were be VBD cord-268467-btfz6ye8 235 5 most most RBS cord-268467-btfz6ye8 235 6 likely likely JJ cord-268467-btfz6ye8 235 7 to to TO cord-268467-btfz6ye8 235 8 represent represent VB cord-268467-btfz6ye8 235 9 the the DT cord-268467-btfz6ye8 235 10 primerextended primerextende VBN cord-268467-btfz6ye8 235 11 products product NNS cord-268467-btfz6ye8 235 12 of of IN cord-268467-btfz6ye8 235 13 the the DT cord-268467-btfz6ye8 235 14 individual individual JJ cord-268467-btfz6ye8 235 15 mRNA mRNA NNP cord-268467-btfz6ye8 235 16 species specie NNS cord-268467-btfz6ye8 235 17 , , , cord-268467-btfz6ye8 235 18 the the DT cord-268467-btfz6ye8 235 19 smallest small JJS cord-268467-btfz6ye8 235 20 and and CC cord-268467-btfz6ye8 235 21 most most RBS cord-268467-btfz6ye8 235 22 abundant abundant JJ cord-268467-btfz6ye8 235 23 band band NN cord-268467-btfz6ye8 235 24 , , , cord-268467-btfz6ye8 235 25 corresponding correspond VBG cord-268467-btfz6ye8 235 26 to to IN cord-268467-btfz6ye8 235 27 the the DT cord-268467-btfz6ye8 235 28 primer primer NN cord-268467-btfz6ye8 235 29 - - HYPH cord-268467-btfz6ye8 235 30 extended extended JJ cord-268467-btfz6ye8 235 31 product product NN cord-268467-btfz6ye8 235 32 of of IN cord-268467-btfz6ye8 235 33 mRNA7 mrna7 NN cord-268467-btfz6ye8 235 34 , , , cord-268467-btfz6ye8 235 35 was be VBD cord-268467-btfz6ye8 235 36 eluted elute VBN cord-268467-btfz6ye8 235 37 and and CC cord-268467-btfz6ye8 235 38 sequenced sequence VBN cord-268467-btfz6ye8 235 39 by by IN cord-268467-btfz6ye8 235 40 the the DT cord-268467-btfz6ye8 235 41 chemical chemical NN cord-268467-btfz6ye8 235 42 modification modification NN cord-268467-btfz6ye8 235 43 method method NN cord-268467-btfz6ye8 235 44 ( ( -LRB- cord-268467-btfz6ye8 235 45 Maxam Maxam NNP cord-268467-btfz6ye8 235 46 and and CC cord-268467-btfz6ye8 235 47 Gilbert Gilbert NNP cord-268467-btfz6ye8 235 48 , , , cord-268467-btfz6ye8 235 49 1977 1977 CD cord-268467-btfz6ye8 235 50 ) ) -RRB- cord-268467-btfz6ye8 235 51 . . . cord-268467-btfz6ye8 236 1 The the DT cord-268467-btfz6ye8 236 2 sequence sequence NN cord-268467-btfz6ye8 236 3 of of IN cord-268467-btfz6ye8 236 4 the the DT cord-268467-btfz6ye8 236 5 3'-end 3'-end CD cord-268467-btfz6ye8 236 6 of of IN cord-268467-btfz6ye8 236 7 the the DT cord-268467-btfz6ye8 236 8 primer primer NN cord-268467-btfz6ye8 236 9 - - HYPH cord-268467-btfz6ye8 236 10 extended extended JJ cord-268467-btfz6ye8 236 11 product product NN cord-268467-btfz6ye8 236 12 was be VBD cord-268467-btfz6ye8 236 13 identical identical JJ cord-268467-btfz6ye8 236 14 to to IN cord-268467-btfz6ye8 236 15 the the DT cord-268467-btfz6ye8 236 16 L8 L8 NNP cord-268467-btfz6ye8 236 17 sequence sequence NN cord-268467-btfz6ye8 236 18 from from IN cord-268467-btfz6ye8 236 19 nucleotides nucleotide NNS cord-268467-btfz6ye8 236 20 129 129 CD cord-268467-btfz6ye8 236 21 to to TO cord-268467-btfz6ye8 236 22 17 17 CD cord-268467-btfz6ye8 236 23 1 1 CD cord-268467-btfz6ye8 236 24 . . . cord-268467-btfz6ye8 237 1 At at IN cord-268467-btfz6ye8 237 2 nucleotide nucleotide JJ cord-268467-btfz6ye8 237 3 128 128 CD cord-268467-btfz6ye8 237 4 , , , cord-268467-btfz6ye8 237 5 immediately immediately RB cord-268467-btfz6ye8 237 6 5 5 CD cord-268467-btfz6ye8 237 7 ' ' '' cord-268467-btfz6ye8 237 8 to to IN cord-268467-btfz6ye8 237 9 the the DT cord-268467-btfz6ye8 237 10 proposed propose VBN cord-268467-btfz6ye8 237 11 leader leader NN cord-268467-btfz6ye8 237 12 mRNA mRNA NNP cord-268467-btfz6ye8 237 13 fusion fusion NN cord-268467-btfz6ye8 237 14 site site NN cord-268467-btfz6ye8 237 15 , , , cord-268467-btfz6ye8 237 16 the the DT cord-268467-btfz6ye8 237 17 sequence sequence NN cord-268467-btfz6ye8 237 18 diverged diverge VBD cord-268467-btfz6ye8 237 19 from from IN cord-268467-btfz6ye8 237 20 the the DT cord-268467-btfz6ye8 237 21 L8 L8 NNP cord-268467-btfz6ye8 237 22 sequence sequence NN cord-268467-btfz6ye8 237 23 and and CC cord-268467-btfz6ye8 237 24 revealed reveal VBD cord-268467-btfz6ye8 237 25 a a DT cord-268467-btfz6ye8 237 26 putative putative JJ cord-268467-btfz6ye8 237 27 60-base 60-base CD cord-268467-btfz6ye8 237 28 leader leader NN cord-268467-btfz6ye8 237 29 sequence sequence NN cord-268467-btfz6ye8 237 30 which which WDT cord-268467-btfz6ye8 237 31 is be VBZ cord-268467-btfz6ye8 237 32 shown show VBN cord-268467-btfz6ye8 237 33 in in IN cord-268467-btfz6ye8 237 34 Fig Fig NNP cord-268467-btfz6ye8 237 35 . . . cord-268467-btfz6ye8 238 1 6 6 LS cord-268467-btfz6ye8 238 2 . . . cord-268467-btfz6ye8 239 1 The the DT cord-268467-btfz6ye8 239 2 figure figure NN cord-268467-btfz6ye8 239 3 also also RB cord-268467-btfz6ye8 239 4 shows show VBZ cord-268467-btfz6ye8 239 5 a a DT cord-268467-btfz6ye8 239 6 degree degree NN cord-268467-btfz6ye8 239 7 of of IN cord-268467-btfz6ye8 239 8 homology homology NN cord-268467-btfz6ye8 239 9 with with IN cord-268467-btfz6ye8 239 10 the the DT cord-268467-btfz6ye8 239 11 leader leader NN cord-268467-btfz6ye8 239 12 sequence sequence NN cord-268467-btfz6ye8 239 13 of of IN cord-268467-btfz6ye8 239 14 IBV IBV NNP cord-268467-btfz6ye8 239 15 . . . cord-268467-btfz6ye8 240 1 Considerably considerably RB cord-268467-btfz6ye8 240 2 less less JJR cord-268467-btfz6ye8 240 3 homology homology NN cord-268467-btfz6ye8 240 4 exists exist VBZ cord-268467-btfz6ye8 240 5 between between IN cord-268467-btfz6ye8 240 6 the the DT cord-268467-btfz6ye8 240 7 leader leader NN cord-268467-btfz6ye8 240 8 sequence sequence NN cord-268467-btfz6ye8 240 9 of of IN cord-268467-btfz6ye8 240 10 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 240 11 and and CC cord-268467-btfz6ye8 240 12 those those DT cord-268467-btfz6ye8 240 13 of of IN cord-268467-btfz6ye8 240 14 HCV HCV NNP cord-268467-btfz6ye8 240 15 - - HYPH cord-268467-btfz6ye8 240 16 OC43 OC43 NNP cord-268467-btfz6ye8 240 17 and and CC cord-268467-btfz6ye8 240 18 MHV MHV NNP cord-268467-btfz6ye8 240 19 - - HYPH cord-268467-btfz6ye8 240 20 JHM JHM NNP cord-268467-btfz6ye8 240 21 ( ( -LRB- cord-268467-btfz6ye8 240 22 data datum NNS cord-268467-btfz6ye8 240 23 not not RB cord-268467-btfz6ye8 240 24 shown show VBN cord-268467-btfz6ye8 240 25 ) ) -RRB- cord-268467-btfz6ye8 240 26 . . . cord-268467-btfz6ye8 241 1 This this DT cord-268467-btfz6ye8 241 2 report report NN cord-268467-btfz6ye8 241 3 presented present VBD cord-268467-btfz6ye8 241 4 the the DT cord-268467-btfz6ye8 241 5 primary primary JJ cord-268467-btfz6ye8 241 6 sequence sequence NN cord-268467-btfz6ye8 241 7 of of IN cord-268467-btfz6ye8 241 8 the the DT cord-268467-btfz6ye8 241 9 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 241 10 gene gene NN cord-268467-btfz6ye8 241 11 and and CC cord-268467-btfz6ye8 241 12 leader leader NN cord-268467-btfz6ye8 241 13 sequence sequence NN cord-268467-btfz6ye8 241 14 of of IN cord-268467-btfz6ye8 241 15 HCV-229E. HCV-229E. NNP cord-268467-btfz6ye8 241 16 When when WRB cord-268467-btfz6ye8 241 17 compared compare VBN cord-268467-btfz6ye8 241 18 to to IN cord-268467-btfz6ye8 241 19 the the DT cord-268467-btfz6ye8 241 20 known know VBN cord-268467-btfz6ye8 241 21 sequences sequence NNS cord-268467-btfz6ye8 241 22 of of IN cord-268467-btfz6ye8 241 23 other other JJ cord-268467-btfz6ye8 241 24 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 241 25 ( ( -LRB- cord-268467-btfz6ye8 241 26 Kapke Kapke NNP cord-268467-btfz6ye8 241 27 and and CC cord-268467-btfz6ye8 241 28 Brian Brian NNP cord-268467-btfz6ye8 241 29 , , , cord-268467-btfz6ye8 241 30 1986 1986 CD cord-268467-btfz6ye8 241 31 ; ; : cord-268467-btfz6ye8 241 32 Skinner Skinner NNP cord-268467-btfz6ye8 241 33 and and CC cord-268467-btfz6ye8 241 34 Siddell Siddell NNP cord-268467-btfz6ye8 241 35 , , , cord-268467-btfz6ye8 241 36 1984 1984 CD cord-268467-btfz6ye8 241 37 ; ; : cord-268467-btfz6ye8 241 38 Armstrong Armstrong NNP cord-268467-btfz6ye8 241 39 et et NNP cord-268467-btfz6ye8 241 40 a/. a/. NNP cord-268467-btfz6ye8 241 41 , , , cord-268467-btfz6ye8 241 42 1983 1983 CD cord-268467-btfz6ye8 241 43 ; ; : cord-268467-btfz6ye8 241 44 Lapps Lapps NNP cord-268467-btfz6ye8 241 45 et et NNP cord-268467-btfz6ye8 241 46 al al NNP cord-268467-btfz6ye8 241 47 . . NNP cord-268467-btfz6ye8 241 48 , , , cord-268467-btfz6ye8 241 49 1987 1987 CD cord-268467-btfz6ye8 241 50 ; ; : cord-268467-btfz6ye8 241 51 Kamahora Kamahora NNP cord-268467-btfz6ye8 241 52 et et NNP cord-268467-btfz6ye8 241 53 al al NNP cord-268467-btfz6ye8 241 54 . . NNP cord-268467-btfz6ye8 241 55 , , , cord-268467-btfz6ye8 241 56 1988 1988 CD cord-268467-btfz6ye8 241 57 ; ; : cord-268467-btfz6ye8 241 58 Boursnell Boursnell NNP cord-268467-btfz6ye8 241 59 et et FW cord-268467-btfz6ye8 241 60 al al NNP cord-268467-btfz6ye8 241 61 . . NNP cord-268467-btfz6ye8 241 62 , , , cord-268467-btfz6ye8 241 63 1985 1985 CD cord-268467-btfz6ye8 241 64 ) ) -RRB- cord-268467-btfz6ye8 241 65 , , , cord-268467-btfz6ye8 241 66 common common JJ cord-268467-btfz6ye8 241 67 features feature NNS cord-268467-btfz6ye8 241 68 of of IN cord-268467-btfz6ye8 241 69 coronavirus coronavirus NN cord-268467-btfz6ye8 241 70 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 241 71 proteins protein NNS cord-268467-btfz6ye8 241 72 emerged emerge VBD cord-268467-btfz6ye8 241 73 ; ; : cord-268467-btfz6ye8 241 74 namely namely RB cord-268467-btfz6ye8 241 75 , , , cord-268467-btfz6ye8 241 76 they -PRON- PRP cord-268467-btfz6ye8 241 77 are be VBP cord-268467-btfz6ye8 241 78 highly highly RB cord-268467-btfz6ye8 241 79 basic basic JJ cord-268467-btfz6ye8 241 80 and and CC cord-268467-btfz6ye8 241 81 have have VBP cord-268467-btfz6ye8 241 82 a a DT cord-268467-btfz6ye8 241 83 high high JJ cord-268467-btfz6ye8 241 84 proportion proportion NN cord-268467-btfz6ye8 241 85 of of IN cord-268467-btfz6ye8 241 86 serine serine JJ cord-268467-btfz6ye8 241 87 residues residue NNS cord-268467-btfz6ye8 241 88 , , , cord-268467-btfz6ye8 241 89 which which WDT cord-268467-btfz6ye8 241 90 have have VBP cord-268467-btfz6ye8 241 91 been be VBN cord-268467-btfz6ye8 241 92 shown show VBN cord-268467-btfz6ye8 241 93 30 30 CD cord-268467-btfz6ye8 241 94 _SP cord-268467-btfz6ye8 241 95 40 40 CD cord-268467-btfz6ye8 241 96 _SP cord-268467-btfz6ye8 241 97 50 50 CD cord-268467-btfz6ye8 241 98 _SP cord-268467-btfz6ye8 241 99 60 60 CD cord-268467-btfz6ye8 241 100 _SP cord-268467-btfz6ye8 242 1 I -PRON- PRP cord-268467-btfz6ye8 242 2 _SP cord-268467-btfz6ye8 243 1 I -PRON- PRP cord-268467-btfz6ye8 243 2 _SP cord-268467-btfz6ye8 244 1 I -PRON- PRP cord-268467-btfz6ye8 244 2 _SP cord-268467-btfz6ye8 245 1 I -PRON- PRP cord-268467-btfz6ye8 245 2 _SP cord-268467-btfz6ye8 246 1 I -PRON- PRP cord-268467-btfz6ye8 246 2 _SP cord-268467-btfz6ye8 247 1 I -PRON- PRP cord-268467-btfz6ye8 247 2 _SP cord-268467-btfz6ye8 248 1 HCV-22 HCV-22 NNP cord-268467-btfz6ye8 248 2 _SP cord-268467-btfz6ye8 248 3 9E 9e NN cord-268467-btfz6ye8 248 4 5'-CTTAAG*TACCTTAT*CTATCTA*CAAATAGAAAAG 5'-cttaag*taccttat*ctatcta*caaatagaaaag CD cord-268467-btfz6ye8 249 1 * * NFP cord-268467-btfz6ye8 249 2 * * NFP cord-268467-btfz6ye8 249 3 TTGCTTTTTAGACTTTGTGTC*TA*CTTC ttgctttttagactttgtgtc*ta*cttc NN cord-268467-btfz6ye8 249 4 . . . cord-268467-btfz6ye8 249 5 . . . cord-268467-btfz6ye8 249 6 . . . cord-268467-btfz6ye8 249 7 . . . cord-268467-btfz6ye8 249 8 . . . cord-268467-btfz6ye8 249 9 . . . cord-268467-btfz6ye8 249 10 . . . cord-268467-btfz6ye8 249 11 . . . cord-268467-btfz6ye8 249 12 . . . cord-268467-btfz6ye8 249 13 . . . cord-268467-btfz6ye8 249 14 . . . cord-268467-btfz6ye8 249 15 . . . cord-268467-btfz6ye8 250 1 : : : cord-268467-btfz6ye8 250 2 : : : cord-268467-btfz6ye8 250 3 : : : cord-268467-btfz6ye8 250 4 : : : cord-268467-btfz6ye8 250 5 : : : cord-268467-btfz6ye8 250 6 : : : cord-268467-btfz6ye8 250 7 : : : cord-268467-btfz6ye8 250 8 : : : cord-268467-btfz6ye8 250 9 : : : cord-268467-btfz6ye8 250 10 : : : cord-268467-btfz6ye8 250 11 : : : cord-268467-btfz6ye8 250 12 : : : cord-268467-btfz6ye8 250 13 : : : cord-268467-btfz6ye8 250 14 : : : cord-268467-btfz6ye8 250 15 : : : cord-268467-btfz6ye8 250 16 : : : cord-268467-btfz6ye8 250 17 : : : cord-268467-btfz6ye8 250 18 : : : cord-268467-btfz6ye8 250 19 : : : cord-268467-btfz6ye8 250 20 : : : cord-268467-btfz6ye8 250 21 : : : cord-268467-btfz6ye8 250 22 : : : cord-268467-btfz6ye8 250 23 : : : cord-268467-btfz6ye8 250 24 . . . cord-268467-btfz6ye8 250 25 . . . cord-268467-btfz6ye8 250 26 : : : cord-268467-btfz6ye8 250 27 : : : cord-268467-btfz6ye8 250 28 : : : cord-268467-btfz6ye8 250 29 : : : cord-268467-btfz6ye8 250 30 : : : cord-268467-btfz6ye8 250 31 : : : cord-268467-btfz6ye8 250 32 : : : cord-268467-btfz6ye8 250 33 IBV IBV NNP cord-268467-btfz6ye8 250 34 5'-ACTTAAGATAGATATTAATATATATCTATTACACTAGCCTTGC**GCTAGATTTTTAA*CTTAACAAA 5'-acttaagatagatattaatatatatctattacactagccttgc**gctagatttttaa*cttaacaaa CD cord-268467-btfz6ye8 250 35 ..... ..... NFP cord-268467-btfz6ye8 251 1 FIG FIG NNP cord-268467-btfz6ye8 251 2 . . . cord-268467-btfz6ye8 252 1 6 6 LS cord-268467-btfz6ye8 252 2 . . . cord-268467-btfz6ye8 253 1 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 253 2 mRNA mRNA NNP cord-268467-btfz6ye8 253 3 leader leader NN cord-268467-btfz6ye8 253 4 sequence sequence NN cord-268467-btfz6ye8 253 5 compared compare VBN cord-268467-btfz6ye8 253 6 to to IN cord-268467-btfz6ye8 253 7 the the DT cord-268467-btfz6ye8 253 8 leader leader NN cord-268467-btfz6ye8 253 9 sequence sequence NN cord-268467-btfz6ye8 253 10 of of IN cord-268467-btfz6ye8 253 11 IBV IBV NNP cord-268467-btfz6ye8 253 12 . . . cord-268467-btfz6ye8 254 1 The the DT cord-268467-btfz6ye8 254 2 IBV IBV NNP cord-268467-btfz6ye8 254 3 leader leader NN cord-268467-btfz6ye8 254 4 extends extend VBZ cord-268467-btfz6ye8 254 5 for for IN cord-268467-btfz6ye8 254 6 at at RB cord-268467-btfz6ye8 254 7 least least RBS cord-268467-btfz6ye8 254 8 16 16 CD cord-268467-btfz6ye8 254 9 nucleotides nucleotide NNS cord-268467-btfz6ye8 254 10 in in IN cord-268467-btfz6ye8 254 11 the the DT cord-268467-btfz6ye8 254 12 3 3 CD cord-268467-btfz6ye8 254 13 ' ' POS cord-268467-btfz6ye8 254 14 direction direction NN cord-268467-btfz6ye8 254 15 . . . cord-268467-btfz6ye8 255 1 to to TO cord-268467-btfz6ye8 255 2 be be VB cord-268467-btfz6ye8 255 3 sites site NNS cord-268467-btfz6ye8 255 4 of of IN cord-268467-btfz6ye8 255 5 phosphorylation phosphorylation NN cord-268467-btfz6ye8 256 1 ( ( -LRB- cord-268467-btfz6ye8 256 2 Stohlman Stohlman NNP cord-268467-btfz6ye8 256 3 and and CC cord-268467-btfz6ye8 256 4 Lai Lai NNP cord-268467-btfz6ye8 256 5 , , , cord-268467-btfz6ye8 256 6 region region NN cord-268467-btfz6ye8 256 7 of of IN cord-268467-btfz6ye8 256 8 46 46 CD cord-268467-btfz6ye8 256 9 % % NN cord-268467-btfz6ye8 256 10 homology homology NN cord-268467-btfz6ye8 256 11 within within IN cord-268467-btfz6ye8 256 12 the the DT cord-268467-btfz6ye8 256 13 amino amino NN cord-268467-btfz6ye8 256 14 - - HYPH cord-268467-btfz6ye8 256 15 terminal terminal NN cord-268467-btfz6ye8 256 16 1979 1979 CD cord-268467-btfz6ye8 256 17 ) ) -RRB- cord-268467-btfz6ye8 256 18 . . . cord-268467-btfz6ye8 257 1 The the DT cord-268467-btfz6ye8 257 2 relationship relationship NN cord-268467-btfz6ye8 257 3 between between IN cord-268467-btfz6ye8 257 4 the the DT cord-268467-btfz6ye8 257 5 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 257 6 one one CD cord-268467-btfz6ye8 257 7 - - HYPH cord-268467-btfz6ye8 257 8 third third NN cord-268467-btfz6ye8 257 9 of of IN cord-268467-btfz6ye8 257 10 the the DT cord-268467-btfz6ye8 257 11 protein protein NN cord-268467-btfz6ye8 257 12 which which WDT cord-268467-btfz6ye8 257 13 extends extend VBZ cord-268467-btfz6ye8 257 14 from from IN cord-268467-btfz6ye8 257 15 residues residue NNS cord-268467-btfz6ye8 257 16 genes gene NNS cord-268467-btfz6ye8 257 17 of of IN cord-268467-btfz6ye8 257 18 WV-229E WV-229E NNP cord-268467-btfz6ye8 257 19 and and CC cord-268467-btfz6ye8 257 20 TGEV TGEV NNP cord-268467-btfz6ye8 257 21 is be VBZ cord-268467-btfz6ye8 257 22 particularly particularly RB cord-268467-btfz6ye8 257 23 interest-29 interest-29 CD cord-268467-btfz6ye8 257 24 to to IN cord-268467-btfz6ye8 257 25 134 134 CD cord-268467-btfz6ye8 257 26 in in IN cord-268467-btfz6ye8 257 27 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 257 28 , , , cord-268467-btfz6ye8 257 29 and and CC cord-268467-btfz6ye8 257 30 41 41 CD cord-268467-btfz6ye8 257 31 to to IN cord-268467-btfz6ye8 257 32 146 146 CD cord-268467-btfz6ye8 257 33 in in IN cord-268467-btfz6ye8 257 34 TGEV TGEV NNP cord-268467-btfz6ye8 257 35 . . . cord-268467-btfz6ye8 258 1 Furing furing NN cord-268467-btfz6ye8 258 2 since since IN cord-268467-btfz6ye8 258 3 the the DT cord-268467-btfz6ye8 258 4 viruses virus NNS cord-268467-btfz6ye8 258 5 are be VBP cord-268467-btfz6ye8 258 6 antigenically antigenically RB cord-268467-btfz6ye8 258 7 related relate VBN cord-268467-btfz6ye8 258 8 ( ( -LRB- cord-268467-btfz6ye8 258 9 Mac Mac NNP cord-268467-btfz6ye8 258 10 - - HYPH cord-268467-btfz6ye8 258 11 thermore thermore NNP cord-268467-btfz6ye8 258 12 , , , cord-268467-btfz6ye8 258 13 approximately approximately RB cord-268467-btfz6ye8 258 14 10 10 CD cord-268467-btfz6ye8 258 15 amino amino NN cord-268467-btfz6ye8 258 16 acids acid NNS cord-268467-btfz6ye8 258 17 downstream downstream IN cord-268467-btfz6ye8 258 18 Naughton Naughton NNP cord-268467-btfz6ye8 258 19 , , , cord-268467-btfz6ye8 258 20 1981 1981 CD cord-268467-btfz6ye8 258 21 ) ) -RRB- cord-268467-btfz6ye8 258 22 . . . cord-268467-btfz6ye8 259 1 The the DT cord-268467-btfz6ye8 259 2 predicted predict VBN cord-268467-btfz6ye8 259 3 molecular molecular JJ cord-268467-btfz6ye8 259 4 weights weight NNS cord-268467-btfz6ye8 259 5 of of IN cord-268467-btfz6ye8 259 6 from from IN cord-268467-btfz6ye8 259 7 the the DT cord-268467-btfz6ye8 259 8 homologous homologous JJ cord-268467-btfz6ye8 259 9 region region NN cord-268467-btfz6ye8 259 10 in in IN cord-268467-btfz6ye8 259 11 both both DT cord-268467-btfz6ye8 259 12 proteins protein NNS cord-268467-btfz6ye8 259 13 lies lie VBZ cord-268467-btfz6ye8 259 14 an an DT cord-268467-btfz6ye8 259 15 the the DT cord-268467-btfz6ye8 259 16 N n CD cord-268467-btfz6ye8 259 17 protein protein NN cord-268467-btfz6ye8 259 18 and and CC cord-268467-btfz6ye8 259 19 the the DT cord-268467-btfz6ye8 259 20 number number NN cord-268467-btfz6ye8 259 21 of of IN cord-268467-btfz6ye8 259 22 potential potential JJ cord-268467-btfz6ye8 259 23 phosphoryla phosphoryla NN cord-268467-btfz6ye8 259 24 - - HYPH cord-268467-btfz6ye8 259 25 area area NN cord-268467-btfz6ye8 259 26 which which WDT cord-268467-btfz6ye8 259 27 is be VBZ cord-268467-btfz6ye8 259 28 abundant abundant JJ cord-268467-btfz6ye8 259 29 in in IN cord-268467-btfz6ye8 259 30 serine serine JJ cord-268467-btfz6ye8 259 31 residues residue NNS cord-268467-btfz6ye8 259 32 , , , cord-268467-btfz6ye8 259 33 suggesting suggest VBG cord-268467-btfz6ye8 259 34 tion tion NN cord-268467-btfz6ye8 259 35 sites site NNS cord-268467-btfz6ye8 259 36 of of IN cord-268467-btfz6ye8 259 37 both both DT cord-268467-btfz6ye8 259 38 viruses virus NNS cord-268467-btfz6ye8 259 39 are be VBP cord-268467-btfz6ye8 259 40 almost almost RB cord-268467-btfz6ye8 259 41 identical identical JJ cord-268467-btfz6ye8 259 42 . . . cord-268467-btfz6ye8 260 1 Although although IN cord-268467-btfz6ye8 260 2 that that IN cord-268467-btfz6ye8 260 3 this this DT cord-268467-btfz6ye8 260 4 may may MD cord-268467-btfz6ye8 260 5 be be VB cord-268467-btfz6ye8 260 6 an an DT cord-268467-btfz6ye8 260 7 important important JJ cord-268467-btfz6ye8 260 8 functional functional JJ cord-268467-btfz6ye8 260 9 domain domain NN cord-268467-btfz6ye8 260 10 of of IN cord-268467-btfz6ye8 260 11 the the DT cord-268467-btfz6ye8 260 12 these these DT cord-268467-btfz6ye8 260 13 two two CD cord-268467-btfz6ye8 260 14 viruses virus NNS cord-268467-btfz6ye8 260 15 have have VBP cord-268467-btfz6ye8 260 16 little little JJ cord-268467-btfz6ye8 260 17 nucleotide nucleotide JJ cord-268467-btfz6ye8 260 18 sequence sequence NN cord-268467-btfz6ye8 260 19 ho ho NN cord-268467-btfz6ye8 260 20 - - HYPH cord-268467-btfz6ye8 260 21 molecule molecule NN cord-268467-btfz6ye8 260 22 . . . cord-268467-btfz6ye8 261 1 To to TO cord-268467-btfz6ye8 261 2 further further RB cord-268467-btfz6ye8 261 3 examine examine VB cord-268467-btfz6ye8 261 4 such such JJ cord-268467-btfz6ye8 261 5 functional functional JJ cord-268467-btfz6ye8 261 6 homolmology homolmology NN cord-268467-btfz6ye8 261 7 between between IN cord-268467-btfz6ye8 261 8 their -PRON- PRP$ cord-268467-btfz6ye8 261 9 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 261 10 genes gene NNS cord-268467-btfz6ye8 261 11 , , , cord-268467-btfz6ye8 261 12 the the DT cord-268467-btfz6ye8 261 13 amino amino NN cord-268467-btfz6ye8 261 14 ogy ogy NN cord-268467-btfz6ye8 261 15 between between IN cord-268467-btfz6ye8 261 16 the the DT cord-268467-btfz6ye8 261 17 two two CD cord-268467-btfz6ye8 261 18 proteins protein NNS cord-268467-btfz6ye8 261 19 , , , cord-268467-btfz6ye8 261 20 hydropathy hydropathy NNP cord-268467-btfz6ye8 261 21 profiles profile NNS cord-268467-btfz6ye8 262 1 acid acid NN cord-268467-btfz6ye8 262 2 sequences sequence NNS cord-268467-btfz6ye8 262 3 are be VBP cord-268467-btfz6ye8 262 4 homologous homologous JJ cord-268467-btfz6ye8 262 5 within within IN cord-268467-btfz6ye8 262 6 a a DT cord-268467-btfz6ye8 262 7 limited limited JJ cord-268467-btfz6ye8 262 8 re re NN cord-268467-btfz6ye8 262 9 - - , cord-268467-btfz6ye8 262 10 were be VBD cord-268467-btfz6ye8 262 11 constructed construct VBN cord-268467-btfz6ye8 262 12 ( ( -LRB- cord-268467-btfz6ye8 262 13 Fig Fig NNP cord-268467-btfz6ye8 262 14 . . NNP cord-268467-btfz6ye8 262 15 7 7 CD cord-268467-btfz6ye8 262 16 ) ) -RRB- cord-268467-btfz6ye8 262 17 . . . cord-268467-btfz6ye8 263 1 The the DT cord-268467-btfz6ye8 263 2 contour contour NN cord-268467-btfz6ye8 263 3 of of IN cord-268467-btfz6ye8 263 4 these these DT cord-268467-btfz6ye8 263 5 plots plot NNS cord-268467-btfz6ye8 263 6 gion gion NNP cord-268467-btfz6ye8 263 7 . . . cord-268467-btfz6ye8 264 1 Amino amino NN cord-268467-btfz6ye8 264 2 acid acid NN cord-268467-btfz6ye8 264 3 sequence sequence NN cord-268467-btfz6ye8 264 4 analysis analysis NN cord-268467-btfz6ye8 264 5 revealed reveal VBD cord-268467-btfz6ye8 264 6 several several JJ cord-268467-btfz6ye8 264 7 suggests suggest VBZ cord-268467-btfz6ye8 264 8 that that IN cord-268467-btfz6ye8 264 9 a a DT cord-268467-btfz6ye8 264 10 certain certain JJ cord-268467-btfz6ye8 264 11 degree degree NN cord-268467-btfz6ye8 264 12 of of IN cord-268467-btfz6ye8 264 13 functional functional JJ cord-268467-btfz6ye8 264 14 homology homology NN cord-268467-btfz6ye8 264 15 structural structural JJ cord-268467-btfz6ye8 264 16 features feature VBZ cord-268467-btfz6ye8 264 17 common common JJ cord-268467-btfz6ye8 264 18 to to IN cord-268467-btfz6ye8 264 19 both both DT cord-268467-btfz6ye8 264 20 viruses virus NNS cord-268467-btfz6ye8 264 21 , , , cord-268467-btfz6ye8 264 22 which which WDT cord-268467-btfz6ye8 264 23 may may MD cord-268467-btfz6ye8 264 24 exists exist VBZ cord-268467-btfz6ye8 264 25 within within IN cord-268467-btfz6ye8 264 26 the the DT cord-268467-btfz6ye8 264 27 first first JJ cord-268467-btfz6ye8 264 28 and and CC cord-268467-btfz6ye8 264 29 last last JJ cord-268467-btfz6ye8 264 30 one one CD cord-268467-btfz6ye8 264 31 - - HYPH cord-268467-btfz6ye8 264 32 third third NN cord-268467-btfz6ye8 264 33 of of IN cord-268467-btfz6ye8 264 34 each each DT cord-268467-btfz6ye8 264 35 molehave molehave JJ cord-268467-btfz6ye8 264 36 functional functional JJ cord-268467-btfz6ye8 264 37 significance significance NN cord-268467-btfz6ye8 264 38 . . . cord-268467-btfz6ye8 265 1 For for IN cord-268467-btfz6ye8 265 2 instance instance NN cord-268467-btfz6ye8 265 3 , , , cord-268467-btfz6ye8 265 4 there there EX cord-268467-btfz6ye8 265 5 is be VBZ cord-268467-btfz6ye8 265 6 a a DT cord-268467-btfz6ye8 265 7 cule cule NN cord-268467-btfz6ye8 265 8 , , , cord-268467-btfz6ye8 265 9 with with IN cord-268467-btfz6ye8 265 10 an an DT cord-268467-btfz6ye8 265 11 additional additional JJ cord-268467-btfz6ye8 265 12 region region NN cord-268467-btfz6ye8 265 13 around around IN cord-268467-btfz6ye8 265 14 position position NN cord-268467-btfz6ye8 265 15 200 200 CD cord-268467-btfz6ye8 265 16 . . . cord-268467-btfz6ye8 266 1 The the DT cord-268467-btfz6ye8 266 2 peak peak NN cord-268467-btfz6ye8 266 3 around around IN cord-268467-btfz6ye8 266 4 position position NN cord-268467-btfz6ye8 266 5 200 200 CD cord-268467-btfz6ye8 266 6 occurs occur VBZ cord-268467-btfz6ye8 266 7 just just RB cord-268467-btfz6ye8 266 8 after after IN cord-268467-btfz6ye8 266 9 the the DT cord-268467-btfz6ye8 266 10 serine serine NN cord-268467-btfz6ye8 266 11 - - HYPH cord-268467-btfz6ye8 266 12 rich rich JJ cord-268467-btfz6ye8 266 13 region region NN cord-268467-btfz6ye8 266 14 of of IN cord-268467-btfz6ye8 266 15 the the DT cord-268467-btfz6ye8 266 16 molecule molecule NN cord-268467-btfz6ye8 266 17 . . . cord-268467-btfz6ye8 267 1 The the DT cord-268467-btfz6ye8 267 2 relative relative JJ cord-268467-btfz6ye8 267 3 conservation conservation NN cord-268467-btfz6ye8 267 4 of of IN cord-268467-btfz6ye8 267 5 these these DT cord-268467-btfz6ye8 267 6 regions region NNS cord-268467-btfz6ye8 267 7 suggests suggest VBZ cord-268467-btfz6ye8 267 8 a a DT cord-268467-btfz6ye8 267 9 possible possible JJ cord-268467-btfz6ye8 267 10 role role NN cord-268467-btfz6ye8 267 11 in in IN cord-268467-btfz6ye8 267 12 the the DT cord-268467-btfz6ye8 267 13 interaction interaction NN cord-268467-btfz6ye8 267 14 of of IN cord-268467-btfz6ye8 267 15 the the DT cord-268467-btfz6ye8 267 16 N n CD cord-268467-btfz6ye8 267 17 protein protein NN cord-268467-btfz6ye8 267 18 with with IN cord-268467-btfz6ye8 267 19 the the DT cord-268467-btfz6ye8 267 20 viral viral JJ cord-268467-btfz6ye8 267 21 genome genome NN cord-268467-btfz6ye8 267 22 . . . cord-268467-btfz6ye8 268 1 Similar similar JJ cord-268467-btfz6ye8 268 2 structural structural JJ cord-268467-btfz6ye8 268 3 features feature NNS cord-268467-btfz6ye8 268 4 exist exist VBP cord-268467-btfz6ye8 268 5 among among IN cord-268467-btfz6ye8 268 6 the the DT cord-268467-btfz6ye8 268 7 N n CD cord-268467-btfz6ye8 268 8 proteins protein NNS cord-268467-btfz6ye8 268 9 of of IN cord-268467-btfz6ye8 268 10 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 268 11 , , , cord-268467-btfz6ye8 268 12 IBV IBV NNP cord-268467-btfz6ye8 268 13 , , , cord-268467-btfz6ye8 268 14 MHV MHV NNP cord-268467-btfz6ye8 268 15 , , , cord-268467-btfz6ye8 268 16 HCV HCV NNP cord-268467-btfz6ye8 268 17 - - HYPH cord-268467-btfz6ye8 268 18 OC43 OC43 NNP cord-268467-btfz6ye8 268 19 , , , cord-268467-btfz6ye8 268 20 and and CC cord-268467-btfz6ye8 268 21 BCV BCV NNP cord-268467-btfz6ye8 268 22 ( ( -LRB- cord-268467-btfz6ye8 268 23 Skinner Skinner NNP cord-268467-btfz6ye8 268 24 and and CC cord-268467-btfz6ye8 268 25 Siddell Siddell NNP cord-268467-btfz6ye8 268 26 , , , cord-268467-btfz6ye8 268 27 1984 1984 CD cord-268467-btfz6ye8 268 28 ; ; : cord-268467-btfz6ye8 268 29 Lapps Lapps NNP cord-268467-btfz6ye8 268 30 eT eT NNP cord-268467-btfz6ye8 269 1 a/. a/. NNP cord-268467-btfz6ye8 269 2 , , , cord-268467-btfz6ye8 269 3 1987 1987 CD cord-268467-btfz6ye8 269 4 ; ; : cord-268467-btfz6ye8 270 1 Kamahora Kamahora NNP cord-268467-btfz6ye8 270 2 et et NNP cord-268467-btfz6ye8 270 3 a/. a/. NN cord-268467-btfz6ye8 270 4 , , , cord-268467-btfz6ye8 270 5 1988 1988 CD cord-268467-btfz6ye8 270 6 ; ; : cord-268467-btfz6ye8 270 7 Boursnell Boursnell NNP cord-268467-btfz6ye8 270 8 et et NNP cord-268467-btfz6ye8 270 9 a/. a/. NNP cord-268467-btfz6ye8 270 10 , , , cord-268467-btfz6ye8 270 11 1985 1985 CD cord-268467-btfz6ye8 270 12 ) ) -RRB- cord-268467-btfz6ye8 270 13 . . . cord-268467-btfz6ye8 271 1 This this DT cord-268467-btfz6ye8 271 2 is be VBZ cord-268467-btfz6ye8 271 3 demonstrated demonstrate VBN cord-268467-btfz6ye8 271 4 by by IN cord-268467-btfz6ye8 271 5 the the DT cord-268467-btfz6ye8 271 6 hydropathy hydropathy JJ cord-268467-btfz6ye8 271 7 profiles profile NNS cord-268467-btfz6ye8 271 8 of of IN cord-268467-btfz6ye8 271 9 these these DT cord-268467-btfz6ye8 271 10 proteins protein NNS cord-268467-btfz6ye8 271 11 , , , cord-268467-btfz6ye8 271 12 which which WDT cord-268467-btfz6ye8 271 13 are be VBP cord-268467-btfz6ye8 271 14 also also RB cord-268467-btfz6ye8 271 15 shown show VBN cord-268467-btfz6ye8 271 16 in in IN cord-268467-btfz6ye8 271 17 Fig Fig NNP cord-268467-btfz6ye8 271 18 . . . cord-268467-btfz6ye8 272 1 7 7 CD cord-268467-btfz6ye8 272 2 . . . cord-268467-btfz6ye8 273 1 Further further JJ cord-268467-btfz6ye8 273 2 studies study NNS cord-268467-btfz6ye8 273 3 are be VBP cord-268467-btfz6ye8 273 4 required require VBN cord-268467-btfz6ye8 273 5 to to TO cord-268467-btfz6ye8 273 6 reveal reveal VB cord-268467-btfz6ye8 273 7 the the DT cord-268467-btfz6ye8 273 8 functional functional JJ cord-268467-btfz6ye8 273 9 significance significance NN cord-268467-btfz6ye8 273 10 of of IN cord-268467-btfz6ye8 273 11 the the DT cord-268467-btfz6ye8 273 12 conserved conserve VBN cord-268467-btfz6ye8 273 13 domains domain NNS cord-268467-btfz6ye8 273 14 . . . cord-268467-btfz6ye8 274 1 Another another DT cord-268467-btfz6ye8 274 2 interesting interesting JJ cord-268467-btfz6ye8 274 3 finding finding NN cord-268467-btfz6ye8 274 4 is be VBZ cord-268467-btfz6ye8 274 5 the the DT cord-268467-btfz6ye8 274 6 open open JJ cord-268467-btfz6ye8 274 7 reading reading NN cord-268467-btfz6ye8 274 8 frame frame NN cord-268467-btfz6ye8 274 9 internal internal JJ cord-268467-btfz6ye8 274 10 to to IN cord-268467-btfz6ye8 274 11 the the DT cord-268467-btfz6ye8 274 12 main main JJ cord-268467-btfz6ye8 274 13 coding code VBG cord-268467-btfz6ye8 274 14 region region NN cord-268467-btfz6ye8 274 15 of of IN cord-268467-btfz6ye8 274 16 the the DT cord-268467-btfz6ye8 274 17 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 274 18 N N NNP cord-268467-btfz6ye8 274 19 gene gene NN cord-268467-btfz6ye8 274 20 . . . cord-268467-btfz6ye8 275 1 Thus thus RB cord-268467-btfz6ye8 275 2 far far RB cord-268467-btfz6ye8 275 3 , , , cord-268467-btfz6ye8 275 4 two two CD cord-268467-btfz6ye8 275 5 other other JJ cord-268467-btfz6ye8 275 6 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 275 7 , , , cord-268467-btfz6ye8 275 8 BCV BCV NNP cord-268467-btfz6ye8 275 9 and and CC cord-268467-btfz6ye8 275 10 MHV MHV NNP cord-268467-btfz6ye8 275 11 - - HYPH cord-268467-btfz6ye8 275 12 JHM JHM NNP cord-268467-btfz6ye8 275 13 , , , cord-268467-btfz6ye8 275 14 have have VBP cord-268467-btfz6ye8 275 15 been be VBN cord-268467-btfz6ye8 275 16 found find VBN cord-268467-btfz6ye8 275 17 to to TO cord-268467-btfz6ye8 275 18 contain contain VB cord-268467-btfz6ye8 275 19 internal internal JJ cord-268467-btfz6ye8 275 20 ORFs orfs NN cord-268467-btfz6ye8 275 21 in in IN cord-268467-btfz6ye8 275 22 gene gene NN cord-268467-btfz6ye8 275 23 7 7 CD cord-268467-btfz6ye8 275 24 ( ( -LRB- cord-268467-btfz6ye8 275 25 Skinner Skinner NNP cord-268467-btfz6ye8 275 26 and and CC cord-268467-btfz6ye8 275 27 Siddell Siddell NNP cord-268467-btfz6ye8 275 28 , , , cord-268467-btfz6ye8 275 29 1984 1984 CD cord-268467-btfz6ye8 275 30 ; ; : cord-268467-btfz6ye8 275 31 Lapps Lapps NNP cord-268467-btfz6ye8 275 32 eta/. eta/. CD cord-268467-btfz6ye8 275 33 , , , cord-268467-btfz6ye8 275 34 1987 1987 CD cord-268467-btfz6ye8 275 35 ) ) -RRB- cord-268467-btfz6ye8 275 36 which which WDT cord-268467-btfz6ye8 275 37 are be VBP cord-268467-btfz6ye8 275 38 preceded precede VBN cord-268467-btfz6ye8 275 39 by by IN cord-268467-btfz6ye8 275 40 optimum optimum JJ cord-268467-btfz6ye8 275 41 translation translation NN cord-268467-btfz6ye8 275 42 initiation initiation NN cord-268467-btfz6ye8 275 43 signals signal NNS cord-268467-btfz6ye8 275 44 according accord VBG cord-268467-btfz6ye8 275 45 to to IN cord-268467-btfz6ye8 275 46 Kozak Kozak NNP cord-268467-btfz6ye8 275 47 's 's POS cord-268467-btfz6ye8 275 48 consensus consensus NN cord-268467-btfz6ye8 275 49 sequence sequence NN cord-268467-btfz6ye8 275 50 ( ( -LRB- cord-268467-btfz6ye8 275 51 Kozak Kozak NNP cord-268467-btfz6ye8 275 52 , , , cord-268467-btfz6ye8 275 53 1983 1983 CD cord-268467-btfz6ye8 275 54 ) ) -RRB- cord-268467-btfz6ye8 275 55 . . . cord-268467-btfz6ye8 276 1 The the DT cord-268467-btfz6ye8 276 2 predicted predict VBN cord-268467-btfz6ye8 276 3 amino amino NN cord-268467-btfz6ye8 276 4 acid acid NN cord-268467-btfz6ye8 276 5 sequences sequence NNS cord-268467-btfz6ye8 276 6 could could MD cord-268467-btfz6ye8 276 7 encode encode VB cord-268467-btfz6ye8 276 8 hypothetical hypothetical JJ cord-268467-btfz6ye8 276 9 proteins protein NNS cord-268467-btfz6ye8 276 10 of of IN cord-268467-btfz6ye8 276 11 molecular molecular JJ cord-268467-btfz6ye8 276 12 weights weight NNS cord-268467-btfz6ye8 276 13 13,973 13,973 CD cord-268467-btfz6ye8 276 14 ; ; : cord-268467-btfz6ye8 276 15 14,842 14,842 CD cord-268467-btfz6ye8 276 16 ; ; : cord-268467-btfz6ye8 276 17 and and CC cord-268467-btfz6ye8 276 18 23,057 23,057 CD cord-268467-btfz6ye8 276 19 for for IN cord-268467-btfz6ye8 276 20 HCV-229E HCV-229E NNP cord-268467-btfz6ye8 276 21 , , , cord-268467-btfz6ye8 276 22 MHV MHV NNP cord-268467-btfz6ye8 276 23 - - HYPH cord-268467-btfz6ye8 276 24 JHM JHM NNP cord-268467-btfz6ye8 276 25 , , , cord-268467-btfz6ye8 276 26 and and CC cord-268467-btfz6ye8 276 27 BCV BCV NNP cord-268467-btfz6ye8 276 28 , , , cord-268467-btfz6ye8 276 29 respectively respectively RB cord-268467-btfz6ye8 276 30 . . . cord-268467-btfz6ye8 277 1 interestingly interestingly RB cord-268467-btfz6ye8 277 2 , , , cord-268467-btfz6ye8 277 3 all all DT cord-268467-btfz6ye8 277 4 three three CD cord-268467-btfz6ye8 277 5 sequences sequence NNS cord-268467-btfz6ye8 277 6 are be VBP cord-268467-btfz6ye8 277 7 abundant abundant JJ cord-268467-btfz6ye8 277 8 in in IN cord-268467-btfz6ye8 277 9 leucine leucine NN cord-268467-btfz6ye8 277 10 residues residue NNS cord-268467-btfz6ye8 277 11 ( ( -LRB- cord-268467-btfz6ye8 277 12 17 17 CD cord-268467-btfz6ye8 277 13 to to TO cord-268467-btfz6ye8 277 14 19 19 CD cord-268467-btfz6ye8 277 15 % % NN cord-268467-btfz6ye8 277 16 ) ) -RRB- cord-268467-btfz6ye8 277 17 . . . cord-268467-btfz6ye8 278 1 HCV HCV NNP cord-268467-btfz6ye8 278 2 - - HYPH cord-268467-btfz6ye8 278 3 OC43 OC43 NNP cord-268467-btfz6ye8 278 4 also also RB cord-268467-btfz6ye8 278 5 has have VBZ cord-268467-btfz6ye8 278 6 two two CD cord-268467-btfz6ye8 278 7 smaller small JJR cord-268467-btfz6ye8 278 8 internal internal JJ cord-268467-btfz6ye8 278 9 ORFs orfs NN cord-268467-btfz6ye8 278 10 encoding encode VBG cord-268467-btfz6ye8 278 11 potential potential JJ cord-268467-btfz6ye8 278 12 leucine leucine NN cord-268467-btfz6ye8 278 13 - - HYPH cord-268467-btfz6ye8 278 14 rich rich JJ cord-268467-btfz6ye8 278 15 proteins protein NNS cord-268467-btfz6ye8 278 16 of of IN cord-268467-btfz6ye8 278 17 8830 8830 CD cord-268467-btfz6ye8 278 18 and and CC cord-268467-btfz6ye8 278 19 16,297 16,297 CD cord-268467-btfz6ye8 278 20 molecular molecular JJ cord-268467-btfz6ye8 278 21 weights weight NNS cord-268467-btfz6ye8 279 1 ( ( -LRB- cord-268467-btfz6ye8 279 2 Kamahora Kamahora NNP cord-268467-btfz6ye8 279 3 et et NNP cord-268467-btfz6ye8 279 4 a/. a/. NNP cord-268467-btfz6ye8 279 5 , , , cord-268467-btfz6ye8 279 6 1988 1988 CD cord-268467-btfz6ye8 279 7 ) ) -RRB- cord-268467-btfz6ye8 279 8 . . . cord-268467-btfz6ye8 280 1 Further further JJ cord-268467-btfz6ye8 280 2 studies study NNS cord-268467-btfz6ye8 280 3 to to TO cord-268467-btfz6ye8 280 4 determine determine VB cord-268467-btfz6ye8 280 5 whether whether IN cord-268467-btfz6ye8 280 6 this this DT cord-268467-btfz6ye8 280 7 hypothetical hypothetical JJ cord-268467-btfz6ye8 280 8 protein protein NN cord-268467-btfz6ye8 280 9 can can MD cord-268467-btfz6ye8 280 10 be be VB cord-268467-btfz6ye8 280 11 detected detect VBN cord-268467-btfz6ye8 280 12 in in IN cord-268467-btfz6ye8 280 13 229E 229e CD cord-268467-btfz6ye8 280 14 - - HYPH cord-268467-btfz6ye8 280 15 infected infect VBN cord-268467-btfz6ye8 280 16 cells cell NNS cord-268467-btfz6ye8 280 17 or or CC cord-268467-btfz6ye8 280 18 by by IN cord-268467-btfz6ye8 280 19 in in FW cord-268467-btfz6ye8 280 20 vitro vitro FW cord-268467-btfz6ye8 280 21 translation translation NN cord-268467-btfz6ye8 280 22 of of IN cord-268467-btfz6ye8 280 23 a a DT cord-268467-btfz6ye8 280 24 full full JJ cord-268467-btfz6ye8 280 25 - - HYPH cord-268467-btfz6ye8 280 26 length length NN cord-268467-btfz6ye8 280 27 cDNA cdna NN cord-268467-btfz6ye8 280 28 clone clone NN cord-268467-btfz6ye8 280 29 ( ( -LRB- cord-268467-btfz6ye8 280 30 i.e. i.e. FW cord-268467-btfz6ye8 280 31 , , , cord-268467-btfz6ye8 280 32 L8 L8 NNP cord-268467-btfz6ye8 280 33 ) ) -RRB- cord-268467-btfz6ye8 280 34 are be VBP cord-268467-btfz6ye8 280 35 in in IN cord-268467-btfz6ye8 280 36 progress progress NN cord-268467-btfz6ye8 280 37 . . . cord-268467-btfz6ye8 281 1 Finally finally RB cord-268467-btfz6ye8 281 2 , , , cord-268467-btfz6ye8 281 3 the the DT cord-268467-btfz6ye8 281 4 3'-noncoding 3'-noncode VBG cord-268467-btfz6ye8 281 5 conserved conserved JJ cord-268467-btfz6ye8 281 6 sequence sequence NN cord-268467-btfz6ye8 281 7 of of IN cord-268467-btfz6ye8 281 8 gene gene NN cord-268467-btfz6ye8 281 9 7 7 CD cord-268467-btfz6ye8 281 10 lends lend VBZ cord-268467-btfz6ye8 281 11 additional additional JJ cord-268467-btfz6ye8 281 12 support support NN cord-268467-btfz6ye8 281 13 to to IN cord-268467-btfz6ye8 281 14 a a DT cord-268467-btfz6ye8 281 15 common common JJ cord-268467-btfz6ye8 281 16 ancestry ancestry NN cord-268467-btfz6ye8 281 17 for for IN cord-268467-btfz6ye8 281 18 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 281 19 , , , cord-268467-btfz6ye8 281 20 regardless regardless RB cord-268467-btfz6ye8 281 21 of of IN cord-268467-btfz6ye8 281 22 antigenic antigenic JJ cord-268467-btfz6ye8 281 23 subgroup subgroup NN cord-268467-btfz6ye8 281 24 . . . cord-268467-btfz6ye8 282 1 This this DT cord-268467-btfz6ye8 282 2 sequence sequence NN cord-268467-btfz6ye8 282 3 has have VBZ cord-268467-btfz6ye8 282 4 been be VBN cord-268467-btfz6ye8 282 5 proposed propose VBN cord-268467-btfz6ye8 282 6 as as IN cord-268467-btfz6ye8 282 7 a a DT cord-268467-btfz6ye8 282 8 recognition recognition NN cord-268467-btfz6ye8 282 9 site site NN cord-268467-btfz6ye8 282 10 for for IN cord-268467-btfz6ye8 282 11 the the DT cord-268467-btfz6ye8 282 12 virus virus NN cord-268467-btfz6ye8 282 13 - - HYPH cord-268467-btfz6ye8 282 14 encoded encode VBN cord-268467-btfz6ye8 282 15 RNA rna NN cord-268467-btfz6ye8 282 16 - - HYPH cord-268467-btfz6ye8 282 17 dependent dependent JJ cord-268467-btfz6ye8 282 18 RNA RNA NNP cord-268467-btfz6ye8 282 19 polymerase polymerase NN cord-268467-btfz6ye8 282 20 prior prior RB cord-268467-btfz6ye8 282 21 to to IN cord-268467-btfz6ye8 282 22 negative negative JJ cord-268467-btfz6ye8 282 23 - - HYPH cord-268467-btfz6ye8 282 24 strand strand NN cord-268467-btfz6ye8 282 25 synthesis synthesis NN cord-268467-btfz6ye8 282 26 ( ( -LRB- cord-268467-btfz6ye8 282 27 Kapke Kapke NNP cord-268467-btfz6ye8 282 28 and and CC cord-268467-btfz6ye8 282 29 Brian Brian NNP cord-268467-btfz6ye8 282 30 , , , cord-268467-btfz6ye8 282 31 1986 1986 CD cord-268467-btfz6ye8 282 32 ) ) -RRB- cord-268467-btfz6ye8 282 33 . . . cord-268467-btfz6ye8 283 1 Certainly certainly RB cord-268467-btfz6ye8 283 2 future future JJ cord-268467-btfz6ye8 283 3 studies study NNS cord-268467-btfz6ye8 283 4 must must MD cord-268467-btfz6ye8 283 5 focus focus VB cord-268467-btfz6ye8 283 6 on on IN cord-268467-btfz6ye8 283 7 examining examine VBG cord-268467-btfz6ye8 283 8 the the DT cord-268467-btfz6ye8 283 9 role role NN cord-268467-btfz6ye8 283 10 of of IN cord-268467-btfz6ye8 283 11 this this DT cord-268467-btfz6ye8 283 12 conserved conserve VBN cord-268467-btfz6ye8 283 13 region region NN cord-268467-btfz6ye8 283 14 in in IN cord-268467-btfz6ye8 283 15 the the DT cord-268467-btfz6ye8 283 16 viral viral JJ cord-268467-btfz6ye8 283 17 replication replication NN cord-268467-btfz6ye8 283 18 cycle cycle NN cord-268467-btfz6ye8 283 19 . . . cord-268467-btfz6ye8 284 1 Sequence sequence NN cord-268467-btfz6ye8 284 2 of of IN cord-268467-btfz6ye8 284 3 the the DT cord-268467-btfz6ye8 284 4 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 284 5 gene gene NN cord-268467-btfz6ye8 284 6 from from IN cord-268467-btfz6ye8 284 7 murine murine JJ cord-268467-btfz6ye8 284 8 coronavirus coronavirus NN cord-268467-btfz6ye8 284 9 MHV mhv NN cord-268467-btfz6ye8 284 10 - - HYPH cord-268467-btfz6ye8 284 11 A59 A59 NNP cord-268467-btfz6ye8 284 12 Characterization characterization NN cord-268467-btfz6ye8 284 13 of of IN cord-268467-btfz6ye8 284 14 leader leader NN cord-268467-btfz6ye8 284 15 - - HYPH cord-268467-btfz6ye8 284 16 related relate VBN cord-268467-btfz6ye8 284 17 small small JJ cord-268467-btfz6ye8 284 18 RNAs rna NNS cord-268467-btfz6ye8 284 19 in in IN cord-268467-btfz6ye8 284 20 coronavirus coronavirus NN cord-268467-btfz6ye8 284 21 - - HYPH cord-268467-btfz6ye8 284 22 infected infect VBN cord-268467-btfz6ye8 284 23 cells cell NNS cord-268467-btfz6ye8 284 24 : : : cord-268467-btfz6ye8 285 1 Further further JJ cord-268467-btfz6ye8 285 2 evidence evidence NN cord-268467-btfz6ye8 285 3 for for IN cord-268467-btfz6ye8 285 4 leader leader NN cord-268467-btfz6ye8 285 5 - - HYPH cord-268467-btfz6ye8 285 6 primed prime VBN cord-268467-btfz6ye8 285 7 mechanism mechanism NN cord-268467-btfz6ye8 285 8 of of IN cord-268467-btfz6ye8 285 9 transcription transcription NN cord-268467-btfz6ye8 285 10 Sequences sequence NNS cord-268467-btfz6ye8 285 11 of of IN cord-268467-btfz6ye8 285 12 the the DT cord-268467-btfz6ye8 285 13 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 285 14 genes gene NNS cord-268467-btfz6ye8 285 15 from from IN cord-268467-btfz6ye8 285 16 two two CD cord-268467-btfz6ye8 285 17 strains strain NNS cord-268467-btfz6ye8 285 18 of of IN cord-268467-btfz6ye8 285 19 avian avian JJ cord-268467-btfz6ye8 285 20 infectious infectious JJ cord-268467-btfz6ye8 285 21 bronchitis bronchitis NN cord-268467-btfz6ye8 285 22 virus virus NN cord-268467-btfz6ye8 285 23 Completion completion NN cord-268467-btfz6ye8 285 24 of of IN cord-268467-btfz6ye8 285 25 the the DT cord-268467-btfz6ye8 285 26 sequence sequence NN cord-268467-btfz6ye8 285 27 of of IN cord-268467-btfz6ye8 285 28 the the DT cord-268467-btfz6ye8 285 29 genome genome NN cord-268467-btfz6ye8 285 30 of of IN cord-268467-btfz6ye8 285 31 the the DT cord-268467-btfz6ye8 285 32 coronavirus coronavirus NN cord-268467-btfz6ye8 285 33 avian avian JJ cord-268467-btfz6ye8 285 34 infectious infectious JJ cord-268467-btfz6ye8 285 35 bronchitis bronchitis NN cord-268467-btfz6ye8 285 36 virus virus NN cord-268467-btfz6ye8 285 37 Characterization characterization NN cord-268467-btfz6ye8 285 38 of of IN cord-268467-btfz6ye8 285 39 two two CD cord-268467-btfz6ye8 285 40 RNA RNA NNP cord-268467-btfz6ye8 285 41 polymerase polymerase NN cord-268467-btfz6ye8 285 42 activities activity NNS cord-268467-btfz6ye8 285 43 induced induce VBN cord-268467-btfz6ye8 285 44 by by IN cord-268467-btfz6ye8 285 45 mouse mouse NNP cord-268467-btfz6ye8 285 46 hepatitis hepatitis NNP cord-268467-btfz6ye8 285 47 virus virus NN cord-268467-btfz6ye8 285 48 . . . cord-268467-btfz6ye8 286 1 1 1 LS cord-268467-btfz6ye8 286 2 . . . cord-268467-btfz6ye8 287 1 Viral Viral NNP cord-268467-btfz6ye8 287 2 Three three CD cord-268467-btfz6ye8 287 3 intergenic intergenic JJ cord-268467-btfz6ye8 287 4 regions region NNS cord-268467-btfz6ye8 287 5 of of IN cord-268467-btfz6ye8 287 6 coronavirus coronavirus NN cord-268467-btfz6ye8 287 7 mouse mouse NN cord-268467-btfz6ye8 287 8 hepatitis hepatitis NN cord-268467-btfz6ye8 287 9 virus virus NN cord-268467-btfz6ye8 287 10 strain strain NN cord-268467-btfz6ye8 288 1 A59 A59 NNP cord-268467-btfz6ye8 288 2 genome genome NN cord-268467-btfz6ye8 288 3 RNA RNA NNP cord-268467-btfz6ye8 288 4 contain contain VBP cord-268467-btfz6ye8 288 5 a a DT cord-268467-btfz6ye8 288 6 common common JJ cord-268467-btfz6ye8 288 7 nucleotide nucleotide JJ cord-268467-btfz6ye8 288 8 sequence sequence NN cord-268467-btfz6ye8 288 9 that that WDT cord-268467-btfz6ye8 288 10 is be VBZ cord-268467-btfz6ye8 288 11 homologous homologous JJ cord-268467-btfz6ye8 288 12 to to IN cord-268467-btfz6ye8 288 13 the the DT cord-268467-btfz6ye8 288 14 3'end 3'end CD cord-268467-btfz6ye8 288 15 of of IN cord-268467-btfz6ye8 288 16 the the DT cord-268467-btfz6ye8 288 17 viral viral JJ cord-268467-btfz6ye8 288 18 mRNA mRNA NNP cord-268467-btfz6ye8 288 19 leader leader NN cord-268467-btfz6ye8 288 20 sequence sequence NN cord-268467-btfz6ye8 288 21 Two two CD cord-268467-btfz6ye8 288 22 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 288 23 isolated isolate VBN cord-268467-btfz6ye8 288 24 from from IN cord-268467-btfz6ye8 288 25 central central JJ cord-268467-btfz6ye8 288 26 nervous nervous JJ cord-268467-btfz6ye8 288 27 system system NN cord-268467-btfz6ye8 288 28 tissue tissue NN cord-268467-btfz6ye8 288 29 of of IN cord-268467-btfz6ye8 288 30 two two CD cord-268467-btfz6ye8 288 31 multiple multiple JJ cord-268467-btfz6ye8 288 32 sclerosis sclerosis NN cord-268467-btfz6ye8 288 33 patients patient NNS cord-268467-btfz6ye8 289 1 Prolonged prolong VBN cord-268467-btfz6ye8 289 2 incubation incubation NN cord-268467-btfz6ye8 289 3 in in IN cord-268467-btfz6ye8 289 4 calcium calcium NN cord-268467-btfz6ye8 289 5 chloride chloride NN cord-268467-btfz6ye8 289 6 improves improve VBZ cord-268467-btfz6ye8 289 7 the the DT cord-268467-btfz6ye8 289 8 competence competence NN cord-268467-btfz6ye8 289 9 of of IN cord-268467-btfz6ye8 289 10 Escherichia Escherichia NNP cord-268467-btfz6ye8 289 11 coli coli NNS cord-268467-btfz6ye8 289 12 cells cell NNS cord-268467-btfz6ye8 289 13 Colony Colony NNP cord-268467-btfz6ye8 289 14 hybridization hybridization NN cord-268467-btfz6ye8 289 15 : : : cord-268467-btfz6ye8 290 1 A a DT cord-268467-btfz6ye8 290 2 method method NN cord-268467-btfz6ye8 290 3 for for IN cord-268467-btfz6ye8 290 4 the the DT cord-268467-btfz6ye8 290 5 isolation isolation NN cord-268467-btfz6ye8 290 6 of of IN cord-268467-btfz6ye8 290 7 cloned clone VBN cord-268467-btfz6ye8 290 8 DNAs dna NNS cord-268467-btfz6ye8 290 9 that that WDT cord-268467-btfz6ye8 290 10 contain contain VBP cord-268467-btfz6ye8 290 11 a a DT cord-268467-btfz6ye8 290 12 specific specific JJ cord-268467-btfz6ye8 290 13 gene gene NN cord-268467-btfz6ye8 290 14 Simple simple JJ cord-268467-btfz6ye8 290 15 and and CC cord-268467-btfz6ye8 290 16 very very RB cord-268467-btfz6ye8 290 17 efficient efficient JJ cord-268467-btfz6ye8 290 18 method method NN cord-268467-btfz6ye8 290 19 for for IN cord-268467-btfz6ye8 290 20 generating generate VBG cord-268467-btfz6ye8 290 21 cDNA cdna NN cord-268467-btfz6ye8 290 22 libraries library NNS cord-268467-btfz6ye8 290 23 Use Use NNP cord-268467-btfz6ye8 290 24 of of IN cord-268467-btfz6ye8 290 25 solubilizable solubilizable JJ cord-268467-btfz6ye8 290 26 acrylamide acrylamide NN cord-268467-btfz6ye8 290 27 disulfide disulfide NN cord-268467-btfz6ye8 290 28 gels gel NNS cord-268467-btfz6ye8 290 29 for for IN cord-268467-btfz6ye8 290 30 isolation isolation NN cord-268467-btfz6ye8 290 31 of of IN cord-268467-btfz6ye8 290 32 DNA dna NN cord-268467-btfz6ye8 290 33 fragments fragment NNS cord-268467-btfz6ye8 290 34 suitable suitable JJ cord-268467-btfz6ye8 290 35 for for IN cord-268467-btfz6ye8 290 36 sequence sequence NN cord-268467-btfz6ye8 290 37 analysis analysis NN cord-268467-btfz6ye8 290 38 Chemical chemical NN cord-268467-btfz6ye8 290 39 and and CC cord-268467-btfz6ye8 290 40 electrophoretic electrophoretic JJ cord-268467-btfz6ye8 290 41 properties property NNS cord-268467-btfz6ye8 290 42 of of IN cord-268467-btfz6ye8 290 43 solubilizable solubilizable JJ cord-268467-btfz6ye8 290 44 disulfide disulfide NN cord-268467-btfz6ye8 290 45 gels gel NNS cord-268467-btfz6ye8 290 46 Purification purification NN cord-268467-btfz6ye8 290 47 and and CC cord-268467-btfz6ye8 290 48 biophysical biophysical JJ cord-268467-btfz6ye8 290 49 properties property NNS cord-268467-btfz6ye8 290 50 of of IN cord-268467-btfz6ye8 290 51 human human JJ cord-268467-btfz6ye8 290 52 coronavirus coronavirus NN cord-268467-btfz6ye8 290 53 229E 229e CD cord-268467-btfz6ye8 291 1 The the DT cord-268467-btfz6ye8 291 2 RNA RNA NNP cord-268467-btfz6ye8 291 3 and and CC cord-268467-btfz6ye8 291 4 proteins protein NNS cord-268467-btfz6ye8 291 5 of of IN cord-268467-btfz6ye8 291 6 human human JJ cord-268467-btfz6ye8 291 7 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 291 8 Sequence Sequence NNP cord-268467-btfz6ye8 291 9 analysis analysis NN cord-268467-btfz6ye8 291 10 of of IN cord-268467-btfz6ye8 291 11 nucleocapsid nucleocapsid NNP cord-268467-btfz6ye8 291 12 gene gene NN cord-268467-btfz6ye8 291 13 and and CC cord-268467-btfz6ye8 291 14 leader leader NN cord-268467-btfz6ye8 291 15 RNA RNA NNP cord-268467-btfz6ye8 291 16 of of IN cord-268467-btfz6ye8 291 17 human human JJ cord-268467-btfz6ye8 291 18 coronavirus coronavirus NN cord-268467-btfz6ye8 291 19 OC43 OC43 NNP cord-268467-btfz6ye8 291 20 Sequence Sequence NNP cord-268467-btfz6ye8 291 21 analysis analysis NN cord-268467-btfz6ye8 291 22 of of IN cord-268467-btfz6ye8 291 23 the the DT cord-268467-btfz6ye8 291 24 porcine porcine JJ cord-268467-btfz6ye8 291 25 transmissable transmissable JJ cord-268467-btfz6ye8 291 26 gastroenteritis gastroenteritis NN cord-268467-btfz6ye8 291 27 coronavirus coronavirus NN cord-268467-btfz6ye8 291 28 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 291 29 protein protein NN cord-268467-btfz6ye8 291 30 gene gene NN cord-268467-btfz6ye8 291 31 Temporal temporal JJ cord-268467-btfz6ye8 291 32 regulation regulation NN cord-268467-btfz6ye8 291 33 of of IN cord-268467-btfz6ye8 291 34 bovine bovine JJ cord-268467-btfz6ye8 291 35 coronavirus coronavirus NN cord-268467-btfz6ye8 291 36 RNA rna NN cord-268467-btfz6ye8 291 37 synthesis synthesis NN cord-268467-btfz6ye8 291 38 Characterization characterization NN cord-268467-btfz6ye8 291 39 of of IN cord-268467-btfz6ye8 291 40 viral viral JJ cord-268467-btfz6ye8 291 41 proteins protein NNS cord-268467-btfz6ye8 291 42 synthesized synthesize VBN cord-268467-btfz6ye8 291 43 in in IN cord-268467-btfz6ye8 291 44 229E 229e CD cord-268467-btfz6ye8 291 45 - - HYPH cord-268467-btfz6ye8 291 46 infected infect VBN cord-268467-btfz6ye8 291 47 cells cell NNS cord-268467-btfz6ye8 291 48 and and CC cord-268467-btfz6ye8 291 49 effect(s effect(s NNP cord-268467-btfz6ye8 291 50 ) ) -RRB- cord-268467-btfz6ye8 291 51 of of IN cord-268467-btfz6ye8 291 52 inhibition inhibition NN cord-268467-btfz6ye8 291 53 of of IN cord-268467-btfz6ye8 291 54 glycosylation glycosylation NN cord-268467-btfz6ye8 291 55 and and CC cord-268467-btfz6ye8 291 56 glycoprotein glycoprotein NN cord-268467-btfz6ye8 291 57 transport transport NN cord-268467-btfz6ye8 291 58 /76 /76 NN cord-268467-btfz6ye8 291 59 ) ) -RRB- cord-268467-btfz6ye8 291 60 . . . cord-268467-btfz6ye8 292 1 Isolation isolation NN cord-268467-btfz6ye8 292 2 and and CC cord-268467-btfz6ye8 292 3 morphology morphology NN cord-268467-btfz6ye8 292 4 of of IN cord-268467-btfz6ye8 292 5 the the DT cord-268467-btfz6ye8 292 6 internal internal JJ cord-268467-btfz6ye8 292 7 component component NN cord-268467-btfz6ye8 292 8 of of IN cord-268467-btfz6ye8 292 9 human human JJ cord-268467-btfz6ye8 292 10 coronavirus coronavirus NN cord-268467-btfz6ye8 292 11 , , , cord-268467-btfz6ye8 292 12 strain strain NN cord-268467-btfz6ye8 292 13 229E 229e CD cord-268467-btfz6ye8 292 14 Bovine bovine JJ cord-268467-btfz6ye8 292 15 coronavirus coronavirus NN cord-268467-btfz6ye8 292 16 hemagglutinin hemagglutinin NN cord-268467-btfz6ye8 292 17 protein protein NN cord-268467-btfz6ye8 292 18 Comparison comparison NN cord-268467-btfz6ye8 292 19 of of IN cord-268467-btfz6ye8 292 20 initiation initiation NN cord-268467-btfz6ye8 292 21 of of IN cord-268467-btfz6ye8 292 22 protein protein NN cord-268467-btfz6ye8 292 23 synthesis synthesis NN cord-268467-btfz6ye8 292 24 in in IN cord-268467-btfz6ye8 292 25 procaryotes procaryotes NNP cord-268467-btfz6ye8 292 26 , , , cord-268467-btfz6ye8 292 27 eucaryotes eucaryote NNS cord-268467-btfz6ye8 292 28 , , , cord-268467-btfz6ye8 292 29 and and CC cord-268467-btfz6ye8 292 30 organelles organelle NNS cord-268467-btfz6ye8 293 1 Replication replication NN cord-268467-btfz6ye8 293 2 of of IN cord-268467-btfz6ye8 293 3 coronavirus coronavirus NN cord-268467-btfz6ye8 293 4 RNA RNA NNP cord-268467-btfz6ye8 293 5 . . . cord-268467-btfz6ye8 294 1 /n /n NNP cord-268467-btfz6ye8 295 1 " " `` cord-268467-btfz6ye8 295 2 RNAGenetits rnagenetit VBZ cord-268467-btfz6ye8 295 3 Characterization characterization NN cord-268467-btfz6ye8 295 4 of of IN cord-268467-btfz6ye8 295 5 leader leader NN cord-268467-btfz6ye8 295 6 RNA RNA NNP cord-268467-btfz6ye8 295 7 sequences sequence NNS cord-268467-btfz6ye8 295 8 on on IN cord-268467-btfz6ye8 295 9 the the DT cord-268467-btfz6ye8 295 10 virion virion NN cord-268467-btfz6ye8 295 11 and and CC cord-268467-btfz6ye8 295 12 mRNAs mrnas CD cord-268467-btfz6ye8 295 13 of of IN cord-268467-btfz6ye8 295 14 mouse mouse NNP cord-268467-btfz6ye8 295 15 hepatitis hepatitis NNP cord-268467-btfz6ye8 295 16 virus virus NN cord-268467-btfz6ye8 295 17 , , , cord-268467-btfz6ye8 295 18 a a DT cord-268467-btfz6ye8 295 19 cytoplasmic cytoplasmic JJ cord-268467-btfz6ye8 295 20 virus virus NN cord-268467-btfz6ye8 295 21 Mouse Mouse NNP cord-268467-btfz6ye8 295 22 hepatitis hepatitis NN cord-268467-btfz6ye8 295 23 virus virus NN cord-268467-btfz6ye8 295 24 A59 A59 NNP cord-268467-btfz6ye8 295 25 : : : cord-268467-btfz6ye8 295 26 Messenger Messenger NNP cord-268467-btfz6ye8 295 27 RNA RNA NNP cord-268467-btfz6ye8 295 28 structure structure NN cord-268467-btfz6ye8 295 29 and and CC cord-268467-btfz6ye8 295 30 genetic genetic JJ cord-268467-btfz6ye8 295 31 localization localization NN cord-268467-btfz6ye8 295 32 of of IN cord-268467-btfz6ye8 295 33 the the DT cord-268467-btfz6ye8 295 34 sequence sequence NN cord-268467-btfz6ye8 295 35 divergence divergence NN cord-268467-btfz6ye8 295 36 from from IN cord-268467-btfz6ye8 295 37 the the DT cord-268467-btfz6ye8 295 38 hepatotropic hepatotropic NNP cord-268467-btfz6ye8 295 39 strain strain NN cord-268467-btfz6ye8 295 40 MHV-3 MHV-3 NNP cord-268467-btfz6ye8 295 41 Coronavirus Coronavirus NNP cord-268467-btfz6ye8 295 42 : : : cord-268467-btfz6ye8 296 1 A a DT cord-268467-btfz6ye8 296 2 jumping jump VBG cord-268467-btfz6ye8 296 3 RNA RNA NNP cord-268467-btfz6ye8 296 4 transcription transcription NN cord-268467-btfz6ye8 296 5 Presence Presence NNP cord-268467-btfz6ye8 296 6 of of IN cord-268467-btfz6ye8 296 7 leader leader NN cord-268467-btfz6ye8 296 8 sequences sequence NNS cord-268467-btfz6ye8 296 9 in in IN cord-268467-btfz6ye8 296 10 the the DT cord-268467-btfz6ye8 296 11 mRNA mRNA NNP cord-268467-btfz6ye8 296 12 of of IN cord-268467-btfz6ye8 296 13 mouse mouse NNP cord-268467-btfz6ye8 296 14 hepatitis hepatitis NNP cord-268467-btfz6ye8 296 15 virus virus NN cord-268467-btfz6ye8 297 1 The the DT cord-268467-btfz6ye8 297 2 RNAof RNAof NNP cord-268467-btfz6ye8 297 3 mouse mouse NN cord-268467-btfz6ye8 297 4 hepatitis hepatitis NN cord-268467-btfz6ye8 297 5 virus virus NN cord-268467-btfz6ye8 297 6 Sequence sequence NN cord-268467-btfz6ye8 297 7 analysis analysis NN cord-268467-btfz6ye8 297 8 of of IN cord-268467-btfz6ye8 297 9 the the DT cord-268467-btfz6ye8 297 10 bovine bovine JJ cord-268467-btfz6ye8 297 11 coronavirus coronavirus NN cord-268467-btfz6ye8 297 12 nucleocapsid nucleocapsid NN cord-268467-btfz6ye8 297 13 and and CC cord-268467-btfz6ye8 297 14 matrix matrix NN cord-268467-btfz6ye8 297 15 protein protein NN cord-268467-btfz6ye8 297 16 genes gene NNS cord-268467-btfz6ye8 298 1 The the DT cord-268467-btfz6ye8 298 2 polypeptides polypeptide NNS cord-268467-btfz6ye8 298 3 of of IN cord-268467-btfz6ye8 298 4 human human NN cord-268467-btfz6ye8 298 5 and and CC cord-268467-btfz6ye8 298 6 mouse mouse NN cord-268467-btfz6ye8 298 7 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 298 8 Structural structural JJ cord-268467-btfz6ye8 298 9 and and CC cord-268467-btfz6ye8 298 10 antigenic antigenic JJ cord-268467-btfz6ye8 298 11 relationships relationship NNS cord-268467-btfz6ye8 298 12 between between IN cord-268467-btfz6ye8 298 13 human human NN cord-268467-btfz6ye8 298 14 , , , cord-268467-btfz6ye8 298 15 murine murine JJ cord-268467-btfz6ye8 298 16 and and CC cord-268467-btfz6ye8 298 17 avian avian JJ cord-268467-btfz6ye8 298 18 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 298 19 Leader Leader NNP cord-268467-btfz6ye8 298 20 sequences sequence NNS cord-268467-btfz6ye8 298 21 of of IN cord-268467-btfz6ye8 298 22 murine murine JJ cord-268467-btfz6ye8 298 23 coronavirus coronavirus NN cord-268467-btfz6ye8 298 24 RNA RNA NNP cord-268467-btfz6ye8 298 25 can can MD cord-268467-btfz6ye8 298 26 be be VB cord-268467-btfz6ye8 298 27 freely freely RB cord-268467-btfz6ye8 298 28 reassorted reassorte VBN cord-268467-btfz6ye8 298 29 : : : cord-268467-btfz6ye8 298 30 Evidence evidence NN cord-268467-btfz6ye8 298 31 for for IN cord-268467-btfz6ye8 298 32 the the DT cord-268467-btfz6ye8 298 33 role role NN cord-268467-btfz6ye8 298 34 of of IN cord-268467-btfz6ye8 298 35 free free JJ cord-268467-btfz6ye8 298 36 leader leader NN cord-268467-btfz6ye8 298 37 RNA RNA NNP cord-268467-btfz6ye8 298 38 in in IN cord-268467-btfz6ye8 298 39 transcription transcription NN cord-268467-btfz6ye8 298 40 Analysis Analysis NNP cord-268467-btfz6ye8 298 41 of of IN cord-268467-btfz6ye8 298 42 genomic genomic JJ cord-268467-btfz6ye8 298 43 and and CC cord-268467-btfz6ye8 298 44 intracellular intracellular JJ cord-268467-btfz6ye8 298 45 viral viral JJ cord-268467-btfz6ye8 298 46 RNAs rna NNS cord-268467-btfz6ye8 298 47 of of IN cord-268467-btfz6ye8 298 48 small small JJ cord-268467-btfz6ye8 298 49 plaque plaque NN cord-268467-btfz6ye8 298 50 mutants mutant NNS cord-268467-btfz6ye8 298 51 of of IN cord-268467-btfz6ye8 298 52 mouse mouse NNP cord-268467-btfz6ye8 298 53 hepatitis hepatitis NNP cord-268467-btfz6ye8 298 54 virus virus NN cord-268467-btfz6ye8 299 1 A a DT cord-268467-btfz6ye8 299 2 new new JJ cord-268467-btfz6ye8 299 3 method method NN cord-268467-btfz6ye8 299 4 for for IN cord-268467-btfz6ye8 299 5 sequencing sequence VBG cord-268467-btfz6ye8 299 6 DNA DNA NNP cord-268467-btfz6ye8 299 7 Sequencing Sequencing NNP cord-268467-btfz6ye8 299 8 end end NN cord-268467-btfz6ye8 299 9 - - HYPH cord-268467-btfz6ye8 299 10 labeled label VBN cord-268467-btfz6ye8 299 11 DNA dna NN cord-268467-btfz6ye8 299 12 with with IN cord-268467-btfz6ye8 299 13 base base NN cord-268467-btfz6ye8 299 14 - - HYPH cord-268467-btfz6ye8 299 15 specific specific JJ cord-268467-btfz6ye8 299 16 chemical chemical NN cord-268467-btfz6ye8 299 17 cleavages cleavage NNS cord-268467-btfz6ye8 300 1 Coronavirus Coronavirus NNP cord-268467-btfz6ye8 300 2 infection infection NN cord-268467-btfz6ye8 300 3 in in IN cord-268467-btfz6ye8 300 4 acute acute JJ cord-268467-btfz6ye8 300 5 lower low JJR cord-268467-btfz6ye8 300 6 respiratory respiratory JJ cord-268467-btfz6ye8 300 7 tract tract NN cord-268467-btfz6ye8 300 8 disease disease NN cord-268467-btfz6ye8 300 9 of of IN cord-268467-btfz6ye8 300 10 infants infant NNS cord-268467-btfz6ye8 300 11 Analysis Analysis NNP cord-268467-btfz6ye8 300 12 of of IN cord-268467-btfz6ye8 300 13 singleand singleand NN cord-268467-btfz6ye8 300 14 double double RB cord-268467-btfz6ye8 300 15 - - HYPH cord-268467-btfz6ye8 300 16 stranded strand VBN cord-268467-btfz6ye8 300 17 nucleic nucleic JJ cord-268467-btfz6ye8 300 18 acids acid NNS cord-268467-btfz6ye8 300 19 on on IN cord-268467-btfz6ye8 300 20 polyacrylamide polyacrylamide NN cord-268467-btfz6ye8 300 21 and and CC cord-268467-btfz6ye8 300 22 agarose agarose JJ cord-268467-btfz6ye8 300 23 gels gel NNS cord-268467-btfz6ye8 300 24 by by IN cord-268467-btfz6ye8 300 25 using use VBG cord-268467-btfz6ye8 300 26 glyoxal glyoxal NNP cord-268467-btfz6ye8 300 27 and and CC cord-268467-btfz6ye8 300 28 acridine acridine NNP cord-268467-btfz6ye8 300 29 orange orange NNP cord-268467-btfz6ye8 300 30 A a DT cord-268467-btfz6ye8 300 31 new new JJ cord-268467-btfz6ye8 300 32 pair pair NN cord-268467-btfz6ye8 300 33 of of IN cord-268467-btfz6ye8 300 34 Ml3 ml3 NN cord-268467-btfz6ye8 300 35 vectors vector NNS cord-268467-btfz6ye8 300 36 for for IN cord-268467-btfz6ye8 300 37 selecting select VBG cord-268467-btfz6ye8 300 38 either either CC cord-268467-btfz6ye8 300 39 DNA dna NN cord-268467-btfz6ye8 300 40 strand strand NN cord-268467-btfz6ye8 300 41 of of IN cord-268467-btfz6ye8 300 42 double double JJ cord-268467-btfz6ye8 300 43 - - HYPH cord-268467-btfz6ye8 300 44 digest digest NN cord-268467-btfz6ye8 300 45 restriction restriction NN cord-268467-btfz6ye8 300 46 fragments fragment NNS cord-268467-btfz6ye8 300 47 A a DT cord-268467-btfz6ye8 300 48 micromethod micromethod NN cord-268467-btfz6ye8 300 49 for for IN cord-268467-btfz6ye8 300 50 detailed detailed JJ cord-268467-btfz6ye8 300 51 characterization characterization NN cord-268467-btfz6ye8 300 52 of of IN cord-268467-btfz6ye8 300 53 high high JJ cord-268467-btfz6ye8 300 54 molecular molecular JJ cord-268467-btfz6ye8 300 55 weight weight NN cord-268467-btfz6ye8 300 56 RNA RNA NNP cord-268467-btfz6ye8 300 57 Antigenic Antigenic NNP cord-268467-btfz6ye8 300 58 relationship relationship NN cord-268467-btfz6ye8 300 59 of of IN cord-268467-btfz6ye8 300 60 the the DT cord-268467-btfz6ye8 300 61 feline feline JJ cord-268467-btfz6ye8 300 62 infectious infectious JJ cord-268467-btfz6ye8 300 63 peritonitis peritonitis NN cord-268467-btfz6ye8 300 64 virus virus NN cord-268467-btfz6ye8 300 65 to to IN cord-268467-btfz6ye8 300 66 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 300 67 of of IN cord-268467-btfz6ye8 300 68 other other JJ cord-268467-btfz6ye8 300 69 species specie NNS cord-268467-btfz6ye8 300 70 Isolation isolation NN cord-268467-btfz6ye8 300 71 and and CC cord-268467-btfz6ye8 300 72 propagation propagation NN cord-268467-btfz6ye8 300 73 of of IN cord-268467-btfz6ye8 300 74 a a DT cord-268467-btfz6ye8 300 75 human human JJ cord-268467-btfz6ye8 300 76 enteric enteric JJ cord-268467-btfz6ye8 300 77 coronavirus coronavirus NN cord-268467-btfz6ye8 300 78 Translation translation NN cord-268467-btfz6ye8 300 79 of of IN cord-268467-btfz6ye8 300 80 three three CD cord-268467-btfz6ye8 300 81 mouse mouse NN cord-268467-btfz6ye8 300 82 hepatitis hepatitis NN cord-268467-btfz6ye8 300 83 virus virus NN cord-268467-btfz6ye8 301 1 strain strain VB cord-268467-btfz6ye8 301 2 A59 A59 NNP cord-268467-btfz6ye8 301 3 subgenomic subgenomic JJ cord-268467-btfz6ye8 301 4 RNAs rna NNS cord-268467-btfz6ye8 301 5 in in IN cord-268467-btfz6ye8 301 6 Xenopuslaevisoocytes Xenopuslaevisoocytes NNP cord-268467-btfz6ye8 301 7 DNA DNA NNP cord-268467-btfz6ye8 301 8 sequencing sequencing NN cord-268467-btfz6ye8 301 9 with with IN cord-268467-btfz6ye8 301 10 chain chain NN cord-268467-btfz6ye8 301 11 - - HYPH cord-268467-btfz6ye8 301 12 terminating terminate VBG cord-268467-btfz6ye8 301 13 inhibitors inhibitor NNS cord-268467-btfz6ye8 302 1 The the DT cord-268467-btfz6ye8 302 2 5'-end 5'-end CD cord-268467-btfz6ye8 302 3 sequence sequence NN cord-268467-btfz6ye8 302 4 of of IN cord-268467-btfz6ye8 302 5 the the DT cord-268467-btfz6ye8 302 6 murine murine JJ cord-268467-btfz6ye8 302 7 coronavirus coronavirus NN cord-268467-btfz6ye8 302 8 genome genome NN cord-268467-btfz6ye8 302 9 : : : cord-268467-btfz6ye8 302 10 Implications implication NNS cord-268467-btfz6ye8 302 11 for for IN cord-268467-btfz6ye8 302 12 multiple multiple JJ cord-268467-btfz6ye8 302 13 fusion fusion NN cord-268467-btfz6ye8 302 14 sites site NNS cord-268467-btfz6ye8 302 15 in in IN cord-268467-btfz6ye8 302 16 leaderprimed leaderprimed CD cord-268467-btfz6ye8 302 17 transcription transcription NN cord-268467-btfz6ye8 302 18 Nucleotide Nucleotide NNP cord-268467-btfz6ye8 302 19 sequencing sequencing NN cord-268467-btfz6ye8 302 20 of of IN cord-268467-btfz6ye8 302 21 mouse mouse NN cord-268467-btfz6ye8 302 22 hepatitis hepatitis NNP cord-268467-btfz6ye8 302 23 virus virus NN cord-268467-btfz6ye8 302 24 strain strain NN cord-268467-btfz6ye8 302 25 JHM JHM NNP cord-268467-btfz6ye8 302 26 messenger messenger NN cord-268467-btfz6ye8 302 27 RNA RNA NNP cord-268467-btfz6ye8 302 28 7 7 CD cord-268467-btfz6ye8 302 29 Phosphoproteins Phosphoproteins NNP cord-268467-btfz6ye8 302 30 of of IN cord-268467-btfz6ye8 302 31 murine murine JJ cord-268467-btfz6ye8 302 32 hepatitis hepatitis NN cord-268467-btfz6ye8 302 33 virus virus NN cord-268467-btfz6ye8 302 34 A a DT cord-268467-btfz6ye8 302 35 bacteriophage bacteriophage NN cord-268467-btfz6ye8 302 36 T7 T7 NNP cord-268467-btfz6ye8 302 37 RNA RNA NNP cord-268467-btfz6ye8 302 38 polymerase polymerase NN cord-268467-btfz6ye8 302 39 / / SYM cord-268467-btfz6ye8 302 40 promoter promoter NN cord-268467-btfz6ye8 302 41 system system NN cord-268467-btfz6ye8 302 42 for for IN cord-268467-btfz6ye8 302 43 controlled control VBN cord-268467-btfz6ye8 302 44 exclusive exclusive JJ cord-268467-btfz6ye8 302 45 expression expression NN cord-268467-btfz6ye8 302 46 of of IN cord-268467-btfz6ye8 302 47 specific specific JJ cord-268467-btfz6ye8 302 48 genes gene NNS cord-268467-btfz6ye8 302 49 Hybridization hybridization NN cord-268467-btfz6ye8 302 50 of of IN cord-268467-btfz6ye8 302 51 denatured denature VBN cord-268467-btfz6ye8 302 52 RNA RNA NNP cord-268467-btfz6ye8 302 53 and and CC cord-268467-btfz6ye8 302 54 small small JJ cord-268467-btfz6ye8 302 55 DNA dna NN cord-268467-btfz6ye8 302 56 fragments fragment NNS cord-268467-btfz6ye8 302 57 transferred transfer VBN cord-268467-btfz6ye8 302 58 to to IN cord-268467-btfz6ye8 302 59 nitrocellulose nitrocellulose NN cord-268467-btfz6ye8 303 1 The the DT cord-268467-btfz6ye8 303 2 biology biology NN cord-268467-btfz6ye8 303 3 and and CC cord-268467-btfz6ye8 303 4 pathogenesis pathogenesis NN cord-268467-btfz6ye8 303 5 of of IN cord-268467-btfz6ye8 303 6 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 304 1 Viral viral JJ cord-268467-btfz6ye8 304 2 models model NNS cord-268467-btfz6ye8 304 3 of of IN cord-268467-btfz6ye8 304 4 demyelination demyelination NN cord-268467-btfz6ye8 304 5 Comparison comparison NN cord-268467-btfz6ye8 304 6 of of IN cord-268467-btfz6ye8 304 7 the the DT cord-268467-btfz6ye8 304 8 RNAs rna NNS cord-268467-btfz6ye8 304 9 of of IN cord-268467-btfz6ye8 304 10 murine murine JJ cord-268467-btfz6ye8 304 11 and and CC cord-268467-btfz6ye8 304 12 human human JJ cord-268467-btfz6ye8 304 13 coronaviruses coronaviruse NNS cord-268467-btfz6ye8 305 1 We -PRON- PRP cord-268467-btfz6ye8 305 2 thank thank VBP cord-268467-btfz6ye8 305 3 Carol Carol NNP cord-268467-btfz6ye8 305 4 Flores Flores NNP cord-268467-btfz6ye8 305 5 for for IN cord-268467-btfz6ye8 305 6 assistance assistance NN cord-268467-btfz6ye8 305 7 in in IN cord-268467-btfz6ye8 305 8 preparation preparation NN cord-268467-btfz6ye8 305 9 of of IN cord-268467-btfz6ye8 305 10 the the DT cord-268467-btfz6ye8 305 11 manuscript manuscript NN cord-268467-btfz6ye8 305 12 . . . cord-268467-btfz6ye8 306 1 This this DT cord-268467-btfz6ye8 306 2 work work NN cord-268467-btfz6ye8 306 3 was be VBD cord-268467-btfz6ye8 306 4 supported support VBN cord-268467-btfz6ye8 306 5 by by IN cord-268467-btfz6ye8 306 6 Public Public NNP cord-268467-btfz6ye8 306 7 Health Health NNP cord-268467-btfz6ye8 306 8 Service Service NNP cord-268467-btfz6ye8 306 9 Research Research NNP cord-268467-btfz6ye8 306 10 Grants Grants NNPS cord-268467-btfz6ye8 306 11 NSl8146 nsl8146 NN cord-268467-btfz6ye8 306 12 and and CC cord-268467-btfz6ye8 306 13 All all DT cord-268467-btfz6ye8 306 14 9244 9244 CD cord-268467-btfz6ye8 306 15 from from IN cord-268467-btfz6ye8 306 16 the the DT cord-268467-btfz6ye8 306 17 National National NNP cord-268467-btfz6ye8 306 18 Institutes Institutes NNPS cord-268467-btfz6ye8 306 19 of of IN cord-268467-btfz6ye8 306 20 Health Health NNP cord-268467-btfz6ye8 306 21 and and CC cord-268467-btfz6ye8 306 22 Grant Grant NNP cord-268467-btfz6ye8 306 23 1449 1449 CD cord-268467-btfz6ye8 306 24 from from IN cord-268467-btfz6ye8 306 25 the the DT cord-268467-btfz6ye8 306 26 National National NNP cord-268467-btfz6ye8 306 27 Multiple Multiple NNP cord-268467-btfz6ye8 306 28 Sclerosis Sclerosis NNP cord-268467-btfz6ye8 306 29 Society Society NNP cord-268467-btfz6ye8 306 30 . . . cord-268467-btfz6ye8 307 1 S.S.S. S.S.S. NNP cord-268467-btfz6ye8 307 2 is be VBZ cord-268467-btfz6ye8 307 3 supported support VBN cord-268467-btfz6ye8 307 4 by by IN cord-268467-btfz6ye8 307 5 a a DT cord-268467-btfz6ye8 307 6 postdoctoral postdoctoral JJ cord-268467-btfz6ye8 307 7 training training NN cord-268467-btfz6ye8 307 8 fellowship fellowship NN cord-268467-btfz6ye8 307 9 from from IN cord-268467-btfz6ye8 307 10 the the DT cord-268467-btfz6ye8 307 11 National National NNP cord-268467-btfz6ye8 307 12 institutes institute NNS cord-268467-btfz6ye8 307 13 of of IN cord-268467-btfz6ye8 307 14 Health Health NNP cord-268467-btfz6ye8 307 15 Grant Grant NNP cord-268467-btfz6ye8 307 16 NS07149 NS07149 NNP cord-268467-btfz6ye8 307 17 . . .