id sid tid token lemma pos cord-274424-juj71nc5 1 1 key key NNP cord-274424-juj71nc5 1 2 : : : cord-274424-juj71nc5 1 3 cord-274424-juj71nc5 cord-274424-juj71nc5 NN cord-274424-juj71nc5 1 4 authors author NNS cord-274424-juj71nc5 1 5 : : : cord-274424-juj71nc5 1 6 Pulford Pulford NNP cord-274424-juj71nc5 1 7 , , , cord-274424-juj71nc5 1 8 David David NNP cord-274424-juj71nc5 1 9 J. J. NNP cord-274424-juj71nc5 1 10 ; ; : cord-274424-juj71nc5 1 11 Britton Britton NNP cord-274424-juj71nc5 1 12 , , , cord-274424-juj71nc5 2 1 Paul Paul NNP cord-274424-juj71nc5 2 2 title title NN cord-274424-juj71nc5 2 3 : : : cord-274424-juj71nc5 3 1 Intracellular Intracellular NNP cord-274424-juj71nc5 3 2 processing processing NN cord-274424-juj71nc5 3 3 of of IN cord-274424-juj71nc5 3 4 the the DT cord-274424-juj71nc5 3 5 porcine porcine JJ cord-274424-juj71nc5 3 6 coronavirus coronavirus NN cord-274424-juj71nc5 3 7 transmissible transmissible JJ cord-274424-juj71nc5 3 8 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 3 9 virus virus NN cord-274424-juj71nc5 3 10 spike spike NN cord-274424-juj71nc5 3 11 protein protein NN cord-274424-juj71nc5 3 12 expressed express VBN cord-274424-juj71nc5 3 13 by by IN cord-274424-juj71nc5 3 14 recombinant recombinant JJ cord-274424-juj71nc5 3 15 vaccinia vaccinia NN cord-274424-juj71nc5 3 16 virus virus NN cord-274424-juj71nc5 3 17 date date NN cord-274424-juj71nc5 3 18 : : : cord-274424-juj71nc5 3 19 1991 1991 CD cord-274424-juj71nc5 3 20 - - SYM cord-274424-juj71nc5 3 21 06 06 CD cord-274424-juj71nc5 3 22 - - SYM cord-274424-juj71nc5 3 23 30 30 CD cord-274424-juj71nc5 3 24 journal journal NN cord-274424-juj71nc5 3 25 : : : cord-274424-juj71nc5 4 1 Virology virology NN cord-274424-juj71nc5 4 2 DOI DOI NNP cord-274424-juj71nc5 4 3 : : : cord-274424-juj71nc5 4 4 10.1016/0042 10.1016/0042 CD cord-274424-juj71nc5 4 5 - - SYM cord-274424-juj71nc5 4 6 6822(91)90617-k 6822(91)90617-k CD cord-274424-juj71nc5 4 7 sha sha NN cord-274424-juj71nc5 4 8 : : : cord-274424-juj71nc5 4 9 8853232527089641a01b16dd0cf45cd2f45275d8 8853232527089641a01b16dd0cf45cd2f45275d8 CD cord-274424-juj71nc5 4 10 doc_id doc_id CD cord-274424-juj71nc5 4 11 : : : cord-274424-juj71nc5 5 1 274424 274424 CD cord-274424-juj71nc5 5 2 cord_uid cord_uid NNS cord-274424-juj71nc5 5 3 : : : cord-274424-juj71nc5 6 1 juj71nc5 juj71nc5 NNP cord-274424-juj71nc5 6 2 Abstract Abstract NNP cord-274424-juj71nc5 7 1 The the DT cord-274424-juj71nc5 7 2 Spike spike NN cord-274424-juj71nc5 7 3 ( ( -LRB- cord-274424-juj71nc5 7 4 S s NN cord-274424-juj71nc5 7 5 ) ) -RRB- cord-274424-juj71nc5 7 6 protein protein NN cord-274424-juj71nc5 7 7 from from IN cord-274424-juj71nc5 7 8 a a DT cord-274424-juj71nc5 7 9 virulent virulent JJ cord-274424-juj71nc5 7 10 British british JJ cord-274424-juj71nc5 7 11 field field NN cord-274424-juj71nc5 7 12 isolate isolate NN cord-274424-juj71nc5 7 13 of of IN cord-274424-juj71nc5 8 1 porcine porcine JJ cord-274424-juj71nc5 8 2 transmissible transmissible JJ cord-274424-juj71nc5 8 3 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 8 4 virus virus NN cord-274424-juj71nc5 8 5 ( ( -LRB- cord-274424-juj71nc5 8 6 TGEV TGEV NNP cord-274424-juj71nc5 8 7 ) ) -RRB- cord-274424-juj71nc5 8 8 FS772/70 FS772/70 NNP cord-274424-juj71nc5 8 9 was be VBD cord-274424-juj71nc5 8 10 constructed construct VBN cord-274424-juj71nc5 8 11 from from IN cord-274424-juj71nc5 8 12 cDNA cdna NN cord-274424-juj71nc5 8 13 and and CC cord-274424-juj71nc5 8 14 inserted insert VBN cord-274424-juj71nc5 8 15 into into IN cord-274424-juj71nc5 8 16 the the DT cord-274424-juj71nc5 8 17 vaccinia vaccinia NN cord-274424-juj71nc5 8 18 virus virus NN cord-274424-juj71nc5 8 19 ( ( -LRB- cord-274424-juj71nc5 8 20 VV VV NNP cord-274424-juj71nc5 8 21 ) ) -RRB- cord-274424-juj71nc5 8 22 thymidine thymidine NN cord-274424-juj71nc5 8 23 kinase kinase NN cord-274424-juj71nc5 8 24 gene gene NN cord-274424-juj71nc5 8 25 locus locus NN cord-274424-juj71nc5 8 26 under under IN cord-274424-juj71nc5 8 27 the the DT cord-274424-juj71nc5 8 28 control control NN cord-274424-juj71nc5 8 29 of of IN cord-274424-juj71nc5 8 30 the the DT cord-274424-juj71nc5 8 31 VV VV NNP cord-274424-juj71nc5 8 32 early early RB cord-274424-juj71nc5 8 33 / / SYM cord-274424-juj71nc5 8 34 late late JJ cord-274424-juj71nc5 8 35 gene gene NN cord-274424-juj71nc5 8 36 P7.5 P7.5 NNP cord-274424-juj71nc5 8 37 K K NNP cord-274424-juj71nc5 8 38 promoter promoter NN cord-274424-juj71nc5 8 39 . . . cord-274424-juj71nc5 9 1 Recombinant Recombinant NNP cord-274424-juj71nc5 9 2 S S NNP cord-274424-juj71nc5 9 3 protein protein NN cord-274424-juj71nc5 9 4 was be VBD cord-274424-juj71nc5 9 5 synthesized synthesize VBN cord-274424-juj71nc5 9 6 as as IN cord-274424-juj71nc5 9 7 an an DT cord-274424-juj71nc5 9 8 endo endo NN cord-274424-juj71nc5 9 9 - - HYPH cord-274424-juj71nc5 9 10 β β NNP cord-274424-juj71nc5 9 11 - - HYPH cord-274424-juj71nc5 9 12 N N NNP cord-274424-juj71nc5 9 13 - - HYPH cord-274424-juj71nc5 9 14 acetylglucosamini acetylglucosamini NNP cord-274424-juj71nc5 9 15 - - HYPH cord-274424-juj71nc5 9 16 dase dase NNP cord-274424-juj71nc5 9 17 H h NN cord-274424-juj71nc5 9 18 ( ( -LRB- cord-274424-juj71nc5 9 19 Endo Endo NNP cord-274424-juj71nc5 9 20 H)-sensitive H)-sensitive NNP cord-274424-juj71nc5 9 21 glycoprotein glycoprotein NN cord-274424-juj71nc5 9 22 with with IN cord-274424-juj71nc5 9 23 high high JJ cord-274424-juj71nc5 9 24 mannose mannose NN cord-274424-juj71nc5 9 25 simple simple JJ cord-274424-juj71nc5 9 26 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 9 27 ( ( -LRB- cord-274424-juj71nc5 9 28 gp190 gp190 NN cord-274424-juj71nc5 9 29 ) ) -RRB- cord-274424-juj71nc5 9 30 that that WDT cord-274424-juj71nc5 9 31 underwent undergo VBD cord-274424-juj71nc5 9 32 post post JJ cord-274424-juj71nc5 9 33 - - JJ cord-274424-juj71nc5 9 34 translational translational JJ cord-274424-juj71nc5 9 35 modification modification NN cord-274424-juj71nc5 9 36 to to IN cord-274424-juj71nc5 9 37 an an DT cord-274424-juj71nc5 9 38 Endo Endo NNP cord-274424-juj71nc5 9 39 H h NN cord-274424-juj71nc5 9 40 - - HYPH cord-274424-juj71nc5 9 41 resistant resistant JJ cord-274424-juj71nc5 9 42 glycoprotein glycoprotein NN cord-274424-juj71nc5 9 43 with with IN cord-274424-juj71nc5 9 44 complex complex JJ cord-274424-juj71nc5 9 45 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 9 46 ( ( -LRB- cord-274424-juj71nc5 9 47 gp210 gp210 NNP cord-274424-juj71nc5 9 48 ) ) -RRB- cord-274424-juj71nc5 9 49 . . . cord-274424-juj71nc5 10 1 Immunofluorescence immunofluorescence NN cord-274424-juj71nc5 10 2 analysis analysis NN cord-274424-juj71nc5 10 3 demonstrated demonstrate VBD cord-274424-juj71nc5 10 4 that that IN cord-274424-juj71nc5 10 5 the the DT cord-274424-juj71nc5 10 6 majority majority NN cord-274424-juj71nc5 10 7 of of IN cord-274424-juj71nc5 10 8 recombinant recombinant JJ cord-274424-juj71nc5 10 9 S S NNP cord-274424-juj71nc5 10 10 protein protein NN cord-274424-juj71nc5 10 11 was be VBD cord-274424-juj71nc5 10 12 retained retain VBN cord-274424-juj71nc5 10 13 at at IN cord-274424-juj71nc5 10 14 the the DT cord-274424-juj71nc5 10 15 Golgi Golgi NNP cord-274424-juj71nc5 10 16 but but CC cord-274424-juj71nc5 10 17 some some DT cord-274424-juj71nc5 10 18 S S NNP cord-274424-juj71nc5 10 19 protein protein NN cord-274424-juj71nc5 10 20 was be VBD cord-274424-juj71nc5 10 21 expressed express VBN cord-274424-juj71nc5 10 22 on on IN cord-274424-juj71nc5 10 23 the the DT cord-274424-juj71nc5 10 24 plasma plasma NN cord-274424-juj71nc5 10 25 membrane membrane NN cord-274424-juj71nc5 10 26 . . . cord-274424-juj71nc5 11 1 Monoclonal monoclonal JJ cord-274424-juj71nc5 11 2 antibodies antibody NNS cord-274424-juj71nc5 11 3 ( ( -LRB- cord-274424-juj71nc5 11 4 mAbs mAbs NNP cord-274424-juj71nc5 11 5 ) ) -RRB- cord-274424-juj71nc5 11 6 raised raise VBD cord-274424-juj71nc5 11 7 against against IN cord-274424-juj71nc5 11 8 native native JJ cord-274424-juj71nc5 11 9 S S NNP cord-274424-juj71nc5 11 10 protein protein NN cord-274424-juj71nc5 11 11 reacted react VBD cord-274424-juj71nc5 11 12 with with IN cord-274424-juj71nc5 11 13 this this DT cord-274424-juj71nc5 11 14 recombinant recombinant JJ cord-274424-juj71nc5 11 15 S s NN cord-274424-juj71nc5 11 16 protein protein NN cord-274424-juj71nc5 11 17 ; ; : cord-274424-juj71nc5 11 18 also also RB cord-274424-juj71nc5 11 19 , , , cord-274424-juj71nc5 11 20 mice mouse NNS cord-274424-juj71nc5 11 21 infected infect VBN cord-274424-juj71nc5 11 22 with with IN cord-274424-juj71nc5 11 23 the the DT cord-274424-juj71nc5 11 24 recombinant recombinant JJ cord-274424-juj71nc5 11 25 vaccinia vaccinia NN cord-274424-juj71nc5 11 26 virus virus NN cord-274424-juj71nc5 11 27 ( ( -LRB- cord-274424-juj71nc5 11 28 rVV rVV NNP cord-274424-juj71nc5 11 29 ) ) -RRB- cord-274424-juj71nc5 11 30 expressing express VBG cord-274424-juj71nc5 11 31 the the DT cord-274424-juj71nc5 11 32 S S NNP cord-274424-juj71nc5 11 33 protein protein NN cord-274424-juj71nc5 11 34 induced induce VBN cord-274424-juj71nc5 11 35 TGEV TGEV NNP cord-274424-juj71nc5 11 36 neutralizing neutralize VBG cord-274424-juj71nc5 11 37 antibodies antibody NNS cord-274424-juj71nc5 11 38 . . . cord-274424-juj71nc5 12 1 A a DT cord-274424-juj71nc5 12 2 truncated truncated JJ cord-274424-juj71nc5 12 3 S s NN cord-274424-juj71nc5 12 4 protein protein NN cord-274424-juj71nc5 12 5 ( ( -LRB- cord-274424-juj71nc5 12 6 SΔ SΔ NNP cord-274424-juj71nc5 12 7 ) ) -RRB- cord-274424-juj71nc5 12 8 was be VBD cord-274424-juj71nc5 12 9 also also RB cord-274424-juj71nc5 12 10 expressed express VBN cord-274424-juj71nc5 12 11 in in IN cord-274424-juj71nc5 12 12 rVV rvv RB cord-274424-juj71nc5 12 13 - - HYPH cord-274424-juj71nc5 12 14 infected infect VBN cord-274424-juj71nc5 12 15 cells cell NNS cord-274424-juj71nc5 12 16 by by IN cord-274424-juj71nc5 12 17 introducing introduce VBG cord-274424-juj71nc5 12 18 a a DT cord-274424-juj71nc5 12 19 deletion deletion NN cord-274424-juj71nc5 12 20 into into IN cord-274424-juj71nc5 12 21 the the DT cord-274424-juj71nc5 12 22 S S NNP cord-274424-juj71nc5 12 23 protein protein NN cord-274424-juj71nc5 12 24 cDNA cdna NN cord-274424-juj71nc5 12 25 that that WDT cord-274424-juj71nc5 12 26 removed remove VBD cord-274424-juj71nc5 12 27 292 292 CD cord-274424-juj71nc5 12 28 amino amino NN cord-274424-juj71nc5 12 29 acids acid NNS cord-274424-juj71nc5 12 30 from from IN cord-274424-juj71nc5 12 31 the the DT cord-274424-juj71nc5 12 32 C C NNP cord-274424-juj71nc5 12 33 - - HYPH cord-274424-juj71nc5 12 34 terminus terminus NN cord-274424-juj71nc5 12 35 . . . cord-274424-juj71nc5 13 1 The the DT cord-274424-juj71nc5 13 2 SΔ SΔ NNP cord-274424-juj71nc5 13 3 protein protein NN cord-274424-juj71nc5 13 4 ( ( -LRB- cord-274424-juj71nc5 13 5 gpl70 gpl70 CD cord-274424-juj71nc5 13 6 ) ) -RRB- cord-274424-juj71nc5 13 7 was be VBD cord-274424-juj71nc5 13 8 shown show VBN cord-274424-juj71nc5 13 9 to to TO cord-274424-juj71nc5 13 10 be be VB cord-274424-juj71nc5 13 11 antigenically antigenically RB cord-274424-juj71nc5 13 12 similar similar JJ cord-274424-juj71nc5 13 13 to to IN cord-274424-juj71nc5 13 14 TGEV TGEV NNP cord-274424-juj71nc5 13 15 S S NNP cord-274424-juj71nc5 13 16 protein protein NN cord-274424-juj71nc5 13 17 by by IN cord-274424-juj71nc5 13 18 immunofluorescence immunofluorescence NN cord-274424-juj71nc5 13 19 and and CC cord-274424-juj71nc5 13 20 immunoprecipitation immunoprecipitation NN cord-274424-juj71nc5 13 21 tests test NNS cord-274424-juj71nc5 13 22 but but CC cord-274424-juj71nc5 13 23 was be VBD cord-274424-juj71nc5 13 24 retained retain VBN cord-274424-juj71nc5 13 25 in in IN cord-274424-juj71nc5 13 26 the the DT cord-274424-juj71nc5 13 27 endoplasmic endoplasmic NN cord-274424-juj71nc5 13 28 reticulum reticulum NN cord-274424-juj71nc5 13 29 and and CC cord-274424-juj71nc5 13 30 not not RB cord-274424-juj71nc5 13 31 expressed express VBN cord-274424-juj71nc5 13 32 on on IN cord-274424-juj71nc5 13 33 the the DT cord-274424-juj71nc5 13 34 cell cell NN cord-274424-juj71nc5 13 35 surface surface NN cord-274424-juj71nc5 13 36 . . . cord-274424-juj71nc5 14 1 Transmissible transmissible JJ cord-274424-juj71nc5 14 2 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 14 3 virus virus NN cord-274424-juj71nc5 14 4 ( ( -LRB- cord-274424-juj71nc5 14 5 TGEV TGEV NNP cord-274424-juj71nc5 14 6 ) ) -RRB- cord-274424-juj71nc5 14 7 belongs belong VBZ cord-274424-juj71nc5 14 8 to to IN cord-274424-juj71nc5 14 9 the the DT cord-274424-juj71nc5 14 10 family family NN cord-274424-juj71nc5 14 11 Coronaviridae Coronaviridae NNP cord-274424-juj71nc5 14 12 , , , cord-274424-juj71nc5 14 13 a a DT cord-274424-juj71nc5 14 14 large large JJ cord-274424-juj71nc5 14 15 group group NN cord-274424-juj71nc5 14 16 of of IN cord-274424-juj71nc5 14 17 enveloped envelop VBN cord-274424-juj71nc5 14 18 viruses virus NNS cord-274424-juj71nc5 14 19 with with IN cord-274424-juj71nc5 14 20 a a DT cord-274424-juj71nc5 14 21 positive positive JJ cord-274424-juj71nc5 14 22 - - HYPH cord-274424-juj71nc5 14 23 stranded strand VBN cord-274424-juj71nc5 14 24 RNA RNA NNP cord-274424-juj71nc5 14 25 genome genome NN cord-274424-juj71nc5 14 26 . . . cord-274424-juj71nc5 15 1 The the DT cord-274424-juj71nc5 15 2 virus virus NN cord-274424-juj71nc5 15 3 causes cause VBZ cord-274424-juj71nc5 15 4 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 15 5 in in IN cord-274424-juj71nc5 15 6 neonatal neonatal JJ cord-274424-juj71nc5 15 7 pigs pig NNS cord-274424-juj71nc5 15 8 , , , cord-274424-juj71nc5 15 9 resulting result VBG cord-274424-juj71nc5 15 10 in in IN cord-274424-juj71nc5 15 11 a a DT cord-274424-juj71nc5 15 12 high high JJ cord-274424-juj71nc5 15 13 mortality mortality NN cord-274424-juj71nc5 15 14 and and CC cord-274424-juj71nc5 15 15 morbidity morbidity NN cord-274424-juj71nc5 15 16 . . . cord-274424-juj71nc5 16 1 TGEVvirions tgevvirion NNS cord-274424-juj71nc5 16 2 are be VBP cord-274424-juj71nc5 16 3 composed compose VBN cord-274424-juj71nc5 16 4 of of IN cord-274424-juj71nc5 16 5 three three CD cord-274424-juj71nc5 16 6 structural structural JJ cord-274424-juj71nc5 16 7 proteins protein NNS cord-274424-juj71nc5 16 8 ; ; : cord-274424-juj71nc5 16 9 a a DT cord-274424-juj71nc5 16 10 basic basic JJ cord-274424-juj71nc5 16 11 phosphorylated phosphorylated JJ cord-274424-juj71nc5 16 12 nucleoprotein nucleoprotein NNP cord-274424-juj71nc5 16 13 ( ( -LRB- cord-274424-juj71nc5 16 14 N N NNP cord-274424-juj71nc5 16 15 ) ) -RRB- cord-274424-juj71nc5 16 16 M M NNP cord-274424-juj71nc5 16 17 , , , cord-274424-juj71nc5 16 18 47,000 47,000 CD cord-274424-juj71nc5 16 19 was be VBD cord-274424-juj71nc5 16 20 shown show VBN cord-274424-juj71nc5 16 21 to to TO cord-274424-juj71nc5 16 22 associate associate VB cord-274424-juj71nc5 16 23 with with IN cord-274424-juj71nc5 16 24 the the DT cord-274424-juj71nc5 16 25 viral viral JJ cord-274424-juj71nc5 16 26 genomic genomic JJ cord-274424-juj71nc5 16 27 RNA RNA NNP cord-274424-juj71nc5 16 28 to to TO cord-274424-juj71nc5 16 29 form form VB cord-274424-juj71nc5 16 30 the the DT cord-274424-juj71nc5 16 31 nucleocapsid nucleocapsid NN cord-274424-juj71nc5 16 32 and and CC cord-274424-juj71nc5 16 33 interact interact VB cord-274424-juj71nc5 16 34 with with IN cord-274424-juj71nc5 16 35 a a DT cord-274424-juj71nc5 16 36 glycosylated glycosylated JJ cord-274424-juj71nc5 16 37 membrane membrane NN cord-274424-juj71nc5 16 38 protein protein NN cord-274424-juj71nc5 16 39 ( ( -LRB- cord-274424-juj71nc5 16 40 M M NNP cord-274424-juj71nc5 16 41 ) ) -RRB- cord-274424-juj71nc5 16 42 observed observe VBD cord-274424-juj71nc5 16 43 as as IN cord-274424-juj71nc5 16 44 a a DT cord-274424-juj71nc5 16 45 series series NN cord-274424-juj71nc5 16 46 of of IN cord-274424-juj71nc5 16 47 polypeptides polypeptide NNS cord-274424-juj71nc5 16 48 n n NNP cord-274424-juj71nc5 16 49 / / SYM cord-274424-juj71nc5 16 50 r r NN cord-274424-juj71nc5 16 51 , , , cord-274424-juj71nc5 16 52 28 28 CD cord-274424-juj71nc5 16 53 - - SYM cord-274424-juj71nc5 16 54 31,000 31,000 CD cord-274424-juj71nc5 16 55 , , , cord-274424-juj71nc5 16 56 and and CC cord-274424-juj71nc5 16 57 the the DT cord-274424-juj71nc5 16 58 peplomer peplomer NN cord-274424-juj71nc5 16 59 or or CC cord-274424-juj71nc5 16 60 spike spike NN cord-274424-juj71nc5 16 61 ( ( -LRB- cord-274424-juj71nc5 16 62 S S NNP cord-274424-juj71nc5 16 63 ) ) -RRB- cord-274424-juj71nc5 16 64 , , , cord-274424-juj71nc5 16 65 a a DT cord-274424-juj71nc5 16 66 surface surface NN cord-274424-juj71nc5 16 67 h h NNP cord-274424-juj71nc5 16 68 / / SYM cord-274424-juj71nc5 16 69 l l NN cord-274424-juj71nc5 16 70 , , , cord-274424-juj71nc5 16 71 200,000 200,000 CD cord-274424-juj71nc5 16 72 glycoprotein glycoprotein NN cord-274424-juj71nc5 16 73 ( ( -LRB- cord-274424-juj71nc5 16 74 Garwes Garwes NNP cord-274424-juj71nc5 16 75 and and CC cord-274424-juj71nc5 16 76 Pocock Pocock NNP cord-274424-juj71nc5 16 77 , , , cord-274424-juj71nc5 16 78 1975 1975 CD cord-274424-juj71nc5 16 79 ) ) -RRB- cord-274424-juj71nc5 16 80 . . . cord-274424-juj71nc5 17 1 The the DT cord-274424-juj71nc5 17 2 TGEV TGEV NNP cord-274424-juj71nc5 17 3 S S NNP cord-274424-juj71nc5 17 4 protein protein NN cord-274424-juj71nc5 17 5 has have VBZ cord-274424-juj71nc5 17 6 been be VBN cord-274424-juj71nc5 17 7 shown show VBN cord-274424-juj71nc5 17 8 to to TO cord-274424-juj71nc5 17 9 elicit elicit VB cord-274424-juj71nc5 17 10 a a DT cord-274424-juj71nc5 17 11 neutralizing neutralize VBG cord-274424-juj71nc5 17 12 antibody antibody NN cord-274424-juj71nc5 17 13 response response NN cord-274424-juj71nc5 17 14 ( ( -LRB- cord-274424-juj71nc5 17 15 Laude Laude NNP cord-274424-juj71nc5 17 16 et et NNP cord-274424-juj71nc5 17 17 a/. a/. NNP cord-274424-juj71nc5 17 18 , , , cord-274424-juj71nc5 17 19 1986 1986 CD cord-274424-juj71nc5 17 20 ; ; : cord-274424-juj71nc5 17 21 Jimenez Jimenez NNP cord-274424-juj71nc5 17 22 et et NNP cord-274424-juj71nc5 17 23 al al NNP cord-274424-juj71nc5 17 24 . . NNP cord-274424-juj71nc5 17 25 , , , cord-274424-juj71nc5 17 26 1986 1986 CD cord-274424-juj71nc5 17 27 ; ; : cord-274424-juj71nc5 17 28 Gatwes Gatwes NNP cord-274424-juj71nc5 17 29 et et NNP cord-274424-juj71nc5 17 30 a/. a/. NN cord-274424-juj71nc5 17 31 , , , cord-274424-juj71nc5 17 32 1987 1987 CD cord-274424-juj71nc5 17 33 ) ) -RRB- cord-274424-juj71nc5 17 34 capable capable JJ cord-274424-juj71nc5 17 35 of of IN cord-274424-juj71nc5 17 36 conferring confer VBG cord-274424-juj71nc5 17 37 some some DT cord-274424-juj71nc5 17 38 protection protection NN cord-274424-juj71nc5 17 39 to to IN cord-274424-juj71nc5 17 40 suckling suckle VBG cord-274424-juj71nc5 17 41 pigs pig NNS cord-274424-juj71nc5 17 42 ( ( -LRB- cord-274424-juj71nc5 17 43 Garwes Garwes NNP cord-274424-juj71nc5 17 44 eta/. eta/. CD cord-274424-juj71nc5 17 45 , , , cord-274424-juj71nc5 17 46 1978/79 1978/79 CD cord-274424-juj71nc5 17 47 ) ) -RRB- cord-274424-juj71nc5 17 48 . . . cord-274424-juj71nc5 18 1 By by IN cord-274424-juj71nc5 18 2 analogy analogy NN cord-274424-juj71nc5 18 3 to to IN cord-274424-juj71nc5 18 4 the the DT cord-274424-juj71nc5 18 5 S S NNP cord-274424-juj71nc5 18 6 protein protein NN cord-274424-juj71nc5 18 7 of of IN cord-274424-juj71nc5 18 8 mouse mouse NNP cord-274424-juj71nc5 18 9 hepatitis hepatitis NNP cord-274424-juj71nc5 18 10 virus virus NN cord-274424-juj71nc5 18 11 ( ( -LRB- cord-274424-juj71nc5 18 12 MHV MHV NNP cord-274424-juj71nc5 18 13 ) ) -RRB- cord-274424-juj71nc5 18 14 , , , cord-274424-juj71nc5 18 15 the the DT cord-274424-juj71nc5 18 16 TGEV TGEV NNP cord-274424-juj71nc5 18 17 S S NNP cord-274424-juj71nc5 18 18 protein protein NN cord-274424-juj71nc5 18 19 may may MD cord-274424-juj71nc5 18 20 possess possess VB cord-274424-juj71nc5 18 21 the the DT cord-274424-juj71nc5 18 22 cell cell NN cord-274424-juj71nc5 18 23 receptor receptor NN cord-274424-juj71nc5 18 24 binding bind VBG cord-274424-juj71nc5 18 25 components component NNS cord-274424-juj71nc5 18 26 ( ( -LRB- cord-274424-juj71nc5 18 27 Collins Collins NNP cord-274424-juj71nc5 18 28 et et NNP cord-274424-juj71nc5 18 29 a/. a/. NN cord-274424-juj71nc5 18 30 , , , cord-274424-juj71nc5 18 31 1982 1982 CD cord-274424-juj71nc5 18 32 ) ) -RRB- cord-274424-juj71nc5 18 33 and and CC cord-274424-juj71nc5 18 34 virulence virulence NN cord-274424-juj71nc5 18 35 determinants determinant NNS cord-274424-juj71nc5 18 36 of of IN cord-274424-juj71nc5 18 37 the the DT cord-274424-juj71nc5 18 38 virus virus NN cord-274424-juj71nc5 19 1 ( ( -LRB- cord-274424-juj71nc5 19 2 Fleming Fleming NNP cord-274424-juj71nc5 19 3 et et NNP cord-274424-juj71nc5 19 4 al al NNP cord-274424-juj71nc5 19 5 . . NNP cord-274424-juj71nc5 19 6 , , , cord-274424-juj71nc5 19 7 1986 1986 CD cord-274424-juj71nc5 19 8 ) ) -RRB- cord-274424-juj71nc5 19 9 . . . cord-274424-juj71nc5 20 1 The the DT cord-274424-juj71nc5 20 2 S S NNP cord-274424-juj71nc5 20 3 protein protein NN cord-274424-juj71nc5 20 4 of of IN cord-274424-juj71nc5 20 5 TGEV TGEV NNP cord-274424-juj71nc5 20 6 and and CC cord-274424-juj71nc5 20 7 coronaviruses coronaviruse NNS cord-274424-juj71nc5 20 8 antigenically antigenically RB cord-274424-juj71nc5 20 9 related relate VBN cord-274424-juj71nc5 20 10 to to IN cord-274424-juj71nc5 20 11 TGEV TGEV NNP cord-274424-juj71nc5 20 12 such such JJ cord-274424-juj71nc5 20 13 as as IN cord-274424-juj71nc5 20 14 feline feline JJ cord-274424-juj71nc5 20 15 infectious infectious JJ cord-274424-juj71nc5 20 16 peritonitis peritonitis NN cord-274424-juj71nc5 20 17 ' ' '' cord-274424-juj71nc5 20 18 Present present JJ cord-274424-juj71nc5 20 19 address address NN cord-274424-juj71nc5 20 20 : : : cord-274424-juj71nc5 20 21 Department Department NNP cord-274424-juj71nc5 20 22 of of IN cord-274424-juj71nc5 20 23 Pathology Pathology NNP cord-274424-juj71nc5 20 24 and and CC cord-274424-juj71nc5 20 25 Microbiology Microbiology NNP cord-274424-juj71nc5 20 26 , , , cord-274424-juj71nc5 20 27 School School NNP cord-274424-juj71nc5 20 28 of of IN cord-274424-juj71nc5 20 29 Medical Medical NNP cord-274424-juj71nc5 20 30 Sciences Sciences NNPS cord-274424-juj71nc5 20 31 , , , cord-274424-juj71nc5 20 32 University University NNP cord-274424-juj71nc5 20 33 of of IN cord-274424-juj71nc5 20 34 Bristol Bristol NNP cord-274424-juj71nc5 20 35 , , , cord-274424-juj71nc5 20 36 University University NNP cord-274424-juj71nc5 20 37 Walk Walk NNP cord-274424-juj71nc5 20 38 , , , cord-274424-juj71nc5 20 39 Bristol Bristol NNP cord-274424-juj71nc5 20 40 , , , cord-274424-juj71nc5 20 41 858 858 CD cord-274424-juj71nc5 20 42 lTD lTD NNP cord-274424-juj71nc5 20 43 , , , cord-274424-juj71nc5 20 44 UK UK NNP cord-274424-juj71nc5 20 45 . . . cord-274424-juj71nc5 21 1 * * NFP cord-274424-juj71nc5 21 2 To to TO cord-274424-juj71nc5 21 3 whom whom WP cord-274424-juj71nc5 21 4 all all DT cord-274424-juj71nc5 21 5 correspondence correspondence NN cord-274424-juj71nc5 21 6 and and CC cord-274424-juj71nc5 21 7 reprint reprint NN cord-274424-juj71nc5 21 8 requests request NNS cord-274424-juj71nc5 21 9 should should MD cord-274424-juj71nc5 21 10 be be VB cord-274424-juj71nc5 21 11 addressed address VBN cord-274424-juj71nc5 21 12 . . . cord-274424-juj71nc5 22 1 virus virus NN cord-274424-juj71nc5 22 2 ( ( -LRB- cord-274424-juj71nc5 22 3 FIPV FIPV NNP cord-274424-juj71nc5 22 4 ) ) -RRB- cord-274424-juj71nc5 22 5 , , , cord-274424-juj71nc5 22 6 canine canine JJ cord-274424-juj71nc5 22 7 coronavirus coronavirus NN cord-274424-juj71nc5 22 8 , , , cord-274424-juj71nc5 22 9 and and CC cord-274424-juj71nc5 22 10 porcine porcine JJ cord-274424-juj71nc5 22 11 respiratory respiratory JJ cord-274424-juj71nc5 22 12 coronavirus coronavirus NN cord-274424-juj71nc5 22 13 , , , cord-274424-juj71nc5 22 14 are be VBP cord-274424-juj71nc5 22 15 not not RB cord-274424-juj71nc5 22 16 cleaved cleave VBN cord-274424-juj71nc5 22 17 ( ( -LRB- cord-274424-juj71nc5 22 18 for for IN cord-274424-juj71nc5 22 19 a a DT cord-274424-juj71nc5 22 20 review review NN cord-274424-juj71nc5 22 21 see see VBP cord-274424-juj71nc5 22 22 Spaan Spaan NNP cord-274424-juj71nc5 22 23 et et FW cord-274424-juj71nc5 22 24 al al NNP cord-274424-juj71nc5 22 25 . . NNP cord-274424-juj71nc5 22 26 , , , cord-274424-juj71nc5 22 27 1988 1988 CD cord-274424-juj71nc5 22 28 ) ) -RRB- cord-274424-juj71nc5 22 29 . . . cord-274424-juj71nc5 23 1 However however RB cord-274424-juj71nc5 23 2 , , , cord-274424-juj71nc5 23 3 the the DT cord-274424-juj71nc5 23 4 S S NNP cord-274424-juj71nc5 23 5 proteins protein NNS cord-274424-juj71nc5 23 6 from from IN cord-274424-juj71nc5 23 7 MHV MHV NNP cord-274424-juj71nc5 23 8 , , , cord-274424-juj71nc5 23 9 infectious infectious JJ cord-274424-juj71nc5 23 10 bronchitis bronchitis NN cord-274424-juj71nc5 23 11 virus virus NN cord-274424-juj71nc5 23 12 , , , cord-274424-juj71nc5 23 13 bovine bovine JJ cord-274424-juj71nc5 23 14 coronavirus coronavirus NN cord-274424-juj71nc5 23 15 , , , cord-274424-juj71nc5 23 16 human human JJ cord-274424-juj71nc5 23 17 coronavirus coronavirus NN cord-274424-juj71nc5 23 18 ( ( -LRB- cord-274424-juj71nc5 23 19 OC43 OC43 NNP cord-274424-juj71nc5 23 20 and and CC cord-274424-juj71nc5 23 21 229E 229e CD cord-274424-juj71nc5 23 22 ) ) -RRB- cord-274424-juj71nc5 23 23 , , , cord-274424-juj71nc5 23 24 and and CC cord-274424-juj71nc5 23 25 porcine porcine JJ cord-274424-juj71nc5 23 26 hemagglutinating hemagglutinate VBG cord-274424-juj71nc5 23 27 encephalomyelitis encephalomyelitis NN cord-274424-juj71nc5 23 28 virus virus NN cord-274424-juj71nc5 23 29 which which WDT cord-274424-juj71nc5 23 30 are be VBP cord-274424-juj71nc5 23 31 not not RB cord-274424-juj71nc5 23 32 antigenically antigenically RB cord-274424-juj71nc5 23 33 related relate VBN cord-274424-juj71nc5 23 34 to to IN cord-274424-juj71nc5 23 35 TGEV TGEV NNP cord-274424-juj71nc5 23 36 are be VBP cord-274424-juj71nc5 23 37 proteolytically proteolytically RB cord-274424-juj71nc5 23 38 cleaved cleave VBN cord-274424-juj71nc5 23 39 into into IN cord-274424-juj71nc5 23 40 two two CD cord-274424-juj71nc5 23 41 subunits subunit NNS cord-274424-juj71nc5 23 42 ( ( -LRB- cord-274424-juj71nc5 23 43 Spaan Spaan NNP cord-274424-juj71nc5 23 44 et et FW cord-274424-juj71nc5 23 45 a/. a/. NN cord-274424-juj71nc5 23 46 , , , cord-274424-juj71nc5 23 47 1988 1988 CD cord-274424-juj71nc5 23 48 ) ) -RRB- cord-274424-juj71nc5 23 49 . . . cord-274424-juj71nc5 24 1 Some some DT cord-274424-juj71nc5 24 2 coronavirus coronavirus NN cord-274424-juj71nc5 24 3 S s NN cord-274424-juj71nc5 24 4 proteins protein NNS cord-274424-juj71nc5 24 5 have have VBP cord-274424-juj71nc5 24 6 been be VBN cord-274424-juj71nc5 24 7 demonstrated demonstrate VBN cord-274424-juj71nc5 24 8 to to TO cord-274424-juj71nc5 24 9 induce induce VB cord-274424-juj71nc5 24 10 cell cell NN cord-274424-juj71nc5 24 11 fusion fusion NN cord-274424-juj71nc5 24 12 ( ( -LRB- cord-274424-juj71nc5 24 13 Collins Collins NNP cord-274424-juj71nc5 24 14 et et NNP cord-274424-juj71nc5 24 15 a/. a/. NN cord-274424-juj71nc5 24 16 , , , cord-274424-juj71nc5 24 17 1982 1982 CD cord-274424-juj71nc5 24 18 ; ; : cord-274424-juj71nc5 24 19 Sturman Sturman NNP cord-274424-juj71nc5 24 20 et et NNP cord-274424-juj71nc5 24 21 al al NNP cord-274424-juj71nc5 24 22 . . NNP cord-274424-juj71nc5 24 23 , , , cord-274424-juj71nc5 24 24 1985 1985 CD cord-274424-juj71nc5 24 25 ; ; : cord-274424-juj71nc5 24 26 de de NNP cord-274424-juj71nc5 24 27 Groot Groot NNP cord-274424-juj71nc5 24 28 eta/. eta/. NN cord-274424-juj71nc5 24 29 , , , cord-274424-juj71nc5 24 30 1989 1989 CD cord-274424-juj71nc5 24 31 ) ) -RRB- cord-274424-juj71nc5 24 32 , , , cord-274424-juj71nc5 24 33 generating generate VBG cord-274424-juj71nc5 24 34 multinucleated multinucleated JJ cord-274424-juj71nc5 24 35 syncytia syncytia NN cord-274424-juj71nc5 24 36 . . . cord-274424-juj71nc5 25 1 The the DT cord-274424-juj71nc5 25 2 complete complete JJ cord-274424-juj71nc5 25 3 amino amino NN cord-274424-juj71nc5 25 4 acid acid NN cord-274424-juj71nc5 25 5 sequences sequence NNS cord-274424-juj71nc5 25 6 for for IN cord-274424-juj71nc5 25 7 the the DT cord-274424-juj71nc5 25 8 S S NNP cord-274424-juj71nc5 25 9 proteins protein NNS cord-274424-juj71nc5 25 10 of of IN cord-274424-juj71nc5 25 11 the the DT cord-274424-juj71nc5 25 12 virulent virulent JJ cord-274424-juj71nc5 25 13 FS772/70 FS772/70 NNP cord-274424-juj71nc5 25 14 strain strain NN cord-274424-juj71nc5 25 15 ) ) -RRB- cord-274424-juj71nc5 25 16 and and CC cord-274424-juj71nc5 25 17 the the DT cord-274424-juj71nc5 25 18 avirulent avirulent NN cord-274424-juj71nc5 25 19 Purdue Purdue NNP cord-274424-juj71nc5 25 20 strain strain NN cord-274424-juj71nc5 25 21 ( ( -LRB- cord-274424-juj71nc5 25 22 Jacobs Jacobs NNP cord-274424-juj71nc5 25 23 et et NNP cord-274424-juj71nc5 25 24 al al NNP cord-274424-juj71nc5 25 25 . . NNP cord-274424-juj71nc5 25 26 , , , cord-274424-juj71nc5 25 27 1987 1987 CD cord-274424-juj71nc5 25 28 ; ; : cord-274424-juj71nc5 25 29 Rasschaert Rasschaert NNP cord-274424-juj71nc5 25 30 and and CC cord-274424-juj71nc5 25 31 Laude Laude NNP cord-274424-juj71nc5 25 32 , , , cord-274424-juj71nc5 25 33 1987 1987 CD cord-274424-juj71nc5 25 34 ) ) -RRB- cord-274424-juj71nc5 25 35 have have VBP cord-274424-juj71nc5 25 36 been be VBN cord-274424-juj71nc5 25 37 published publish VBN cord-274424-juj71nc5 25 38 and and CC cord-274424-juj71nc5 25 39 compared compare VBN cord-274424-juj71nc5 25 40 with with IN cord-274424-juj71nc5 25 41 other other JJ cord-274424-juj71nc5 25 42 coronaviruses coronaviruse NNS cord-274424-juj71nc5 25 43 . . . cord-274424-juj71nc5 26 1 The the DT cord-274424-juj71nc5 26 2 FS772/70 FS772/70 NNP cord-274424-juj71nc5 26 3 strain strain NN cord-274424-juj71nc5 26 4 S s NN cord-274424-juj71nc5 26 5 protein protein NN cord-274424-juj71nc5 26 6 has have VBZ cord-274424-juj71nc5 26 7 a a DT cord-274424-juj71nc5 26 8 1449-amino 1449-amino CD cord-274424-juj71nc5 26 9 acid acid NN cord-274424-juj71nc5 26 10 precursor precursor NN cord-274424-juj71nc5 26 11 polypeptide polypeptide NN cord-274424-juj71nc5 26 12 with with IN cord-274424-juj71nc5 26 13 33 33 CD cord-274424-juj71nc5 26 14 potential potential JJ cord-274424-juj71nc5 26 15 N n CD cord-274424-juj71nc5 26 16 - - HYPH cord-274424-juj71nc5 26 17 linked link VBN cord-274424-juj71nc5 26 18 glycosylation glycosylation NN cord-274424-juj71nc5 26 19 sites site NNS cord-274424-juj71nc5 26 20 and and CC cord-274424-juj71nc5 26 21 97 97 CD cord-274424-juj71nc5 26 22 % % NN cord-274424-juj71nc5 26 23 sequence sequence NN cord-274424-juj71nc5 26 24 homology homology NN cord-274424-juj71nc5 26 25 at at IN cord-274424-juj71nc5 26 26 the the DT cord-274424-juj71nc5 26 27 nucleotide nucleotide NN cord-274424-juj71nc5 26 28 and and CC cord-274424-juj71nc5 26 29 the the DT cord-274424-juj71nc5 26 30 amino amino NN cord-274424-juj71nc5 26 31 acid acid NN cord-274424-juj71nc5 26 32 level level NN cord-274424-juj71nc5 26 33 with with IN cord-274424-juj71nc5 26 34 the the DT cord-274424-juj71nc5 26 35 Purdue Purdue NNP cord-274424-juj71nc5 26 36 strain strain NN cord-274424-juj71nc5 26 37 . . . cord-274424-juj71nc5 27 1 From from IN cord-274424-juj71nc5 27 2 the the DT cord-274424-juj71nc5 27 3 deduced deduce VBN cord-274424-juj71nc5 27 4 amino amino NN cord-274424-juj71nc5 27 5 acid acid NN cord-274424-juj71nc5 27 6 sequence sequence NN cord-274424-juj71nc5 27 7 the the DT cord-274424-juj71nc5 27 8 coronavirus coronavirus NN cord-274424-juj71nc5 28 1 S s NN cord-274424-juj71nc5 28 2 protein protein NN cord-274424-juj71nc5 28 3 was be VBD cord-274424-juj71nc5 28 4 shown show VBN cord-274424-juj71nc5 28 5 to to TO cord-274424-juj71nc5 28 6 contain contain VB cord-274424-juj71nc5 28 7 characteristic characteristic JJ cord-274424-juj71nc5 28 8 features feature NNS cord-274424-juj71nc5 28 9 : : : cord-274424-juj71nc5 28 10 a a DT cord-274424-juj71nc5 28 11 16-amino 16-amino CD cord-274424-juj71nc5 28 12 acid acid JJ cord-274424-juj71nc5 28 13 cleavable cleavable JJ cord-274424-juj71nc5 28 14 secretory secretory JJ cord-274424-juj71nc5 28 15 signal signal NN cord-274424-juj71nc5 28 16 ; ; : cord-274424-juj71nc5 28 17 a a DT cord-274424-juj71nc5 28 18 heptad heptad NN cord-274424-juj71nc5 28 19 repeat repeat NN cord-274424-juj71nc5 28 20 sequence sequence NN cord-274424-juj71nc5 28 21 that that WDT cord-274424-juj71nc5 28 22 forms form VBZ cord-274424-juj71nc5 28 23 a a DT cord-274424-juj71nc5 28 24 - - HYPH cord-274424-juj71nc5 28 25 helical helical JJ cord-274424-juj71nc5 28 26 structures structure NNS cord-274424-juj71nc5 28 27 which which WDT cord-274424-juj71nc5 28 28 may may MD cord-274424-juj71nc5 28 29 interact interact VB cord-274424-juj71nc5 28 30 with with IN cord-274424-juj71nc5 28 31 other other JJ cord-274424-juj71nc5 28 32 subunits subunit NNS cord-274424-juj71nc5 28 33 to to TO cord-274424-juj71nc5 28 34 form form VB cord-274424-juj71nc5 28 35 a a DT cord-274424-juj71nc5 28 36 coiled coil VBN cord-274424-juj71nc5 28 37 - - HYPH cord-274424-juj71nc5 28 38 coil coil NN cord-274424-juj71nc5 28 39 oligomeric oligomeric JJ cord-274424-juj71nc5 28 40 structure structure NN cord-274424-juj71nc5 28 41 ; ; : cord-274424-juj71nc5 28 42 a a DT cord-274424-juj71nc5 28 43 hydrophobic hydrophobic JJ cord-274424-juj71nc5 28 44 sequence sequence NN cord-274424-juj71nc5 28 45 near near IN cord-274424-juj71nc5 28 46 the the DT cord-274424-juj71nc5 28 47 C c NN cord-274424-juj71nc5 28 48 - - HYPH cord-274424-juj71nc5 28 49 terminus terminus NN cord-274424-juj71nc5 28 50 that that WDT cord-274424-juj71nc5 28 51 is be VBZ cord-274424-juj71nc5 28 52 probably probably RB cord-274424-juj71nc5 28 53 responsible responsible JJ cord-274424-juj71nc5 28 54 for for IN cord-274424-juj71nc5 28 55 anchoring anchor VBG cord-274424-juj71nc5 28 56 the the DT cord-274424-juj71nc5 28 57 S S NNP cord-274424-juj71nc5 28 58 protein protein NN cord-274424-juj71nc5 28 59 to to IN cord-274424-juj71nc5 28 60 the the DT cord-274424-juj71nc5 28 61 virion virion NN cord-274424-juj71nc5 28 62 envelope envelope NN cord-274424-juj71nc5 28 63 . . . cord-274424-juj71nc5 29 1 This this DT cord-274424-juj71nc5 29 2 membrane membrane NN cord-274424-juj71nc5 29 3 anchor anchor NN cord-274424-juj71nc5 29 4 region region NN cord-274424-juj71nc5 29 5 is be VBZ cord-274424-juj71nc5 29 6 imme imme NN cord-274424-juj71nc5 29 7 - - HYPH cord-274424-juj71nc5 29 8 diately diately NNP cord-274424-juj71nc5 29 9 followed follow VBN cord-274424-juj71nc5 29 10 by by IN cord-274424-juj71nc5 29 11 a a DT cord-274424-juj71nc5 29 12 cysteine cysteine NN cord-274424-juj71nc5 29 13 - - HYPH cord-274424-juj71nc5 29 14 rich rich JJ cord-274424-juj71nc5 29 15 domain domain NN cord-274424-juj71nc5 29 16 , , , cord-274424-juj71nc5 29 17 a a DT cord-274424-juj71nc5 29 18 feature feature NN cord-274424-juj71nc5 29 19 common common JJ cord-274424-juj71nc5 29 20 to to IN cord-274424-juj71nc5 29 21 all all DT cord-274424-juj71nc5 29 22 other other JJ cord-274424-juj71nc5 29 23 coronaviruses coronaviruse NNS cord-274424-juj71nc5 29 24 , , , cord-274424-juj71nc5 29 25 that that WDT cord-274424-juj71nc5 29 26 may may MD cord-274424-juj71nc5 29 27 stabilize stabilize VB cord-274424-juj71nc5 29 28 protein protein NN cord-274424-juj71nc5 29 29 - - HYPH cord-274424-juj71nc5 29 30 lipid lipid NN cord-274424-juj71nc5 29 31 interactions interaction NNS cord-274424-juj71nc5 29 32 . . . cord-274424-juj71nc5 30 1 In in IN cord-274424-juj71nc5 30 2 this this DT cord-274424-juj71nc5 30 3 paper paper NN cord-274424-juj71nc5 30 4 we -PRON- PRP cord-274424-juj71nc5 30 5 report report VBP cord-274424-juj71nc5 30 6 the the DT cord-274424-juj71nc5 30 7 construction construction NN cord-274424-juj71nc5 30 8 of of IN cord-274424-juj71nc5 30 9 a a DT cord-274424-juj71nc5 30 10 FS772/ FS772/ NNP cord-274424-juj71nc5 30 11 70 70 CD cord-274424-juj71nc5 30 12 cDNA cdna NN cord-274424-juj71nc5 30 13 S s NN cord-274424-juj71nc5 30 14 gene gene NN cord-274424-juj71nc5 30 15 and and CC cord-274424-juj71nc5 30 16 its -PRON- PRP$ cord-274424-juj71nc5 30 17 expression expression NN cord-274424-juj71nc5 30 18 by by IN cord-274424-juj71nc5 30 19 a a DT cord-274424-juj71nc5 30 20 recombinant recombinant JJ cord-274424-juj71nc5 30 21 vaccina vaccina NN cord-274424-juj71nc5 30 22 virus virus NN cord-274424-juj71nc5 30 23 ( ( -LRB- cord-274424-juj71nc5 30 24 rVV rVV NNP cord-274424-juj71nc5 30 25 ) ) -RRB- cord-274424-juj71nc5 30 26 to to TO cord-274424-juj71nc5 30 27 study study VB cord-274424-juj71nc5 30 28 the the DT cord-274424-juj71nc5 30 29 antigenicity antigenicity NN cord-274424-juj71nc5 30 30 and and CC cord-274424-juj71nc5 30 31 cellular cellular JJ cord-274424-juj71nc5 30 32 localization localization NN cord-274424-juj71nc5 30 33 of of IN cord-274424-juj71nc5 30 34 the the DT cord-274424-juj71nc5 30 35 S S NNP cord-274424-juj71nc5 30 36 protein protein NN cord-274424-juj71nc5 30 37 . . . cord-274424-juj71nc5 31 1 The the DT cord-274424-juj71nc5 31 2 role role NN cord-274424-juj71nc5 31 3 of of IN cord-274424-juj71nc5 31 4 the the DT cord-274424-juj71nc5 31 5 membrane membrane NN cord-274424-juj71nc5 31 6 anchor anchor NN cord-274424-juj71nc5 31 7 domain domain NN cord-274424-juj71nc5 31 8 was be VBD cord-274424-juj71nc5 31 9 investigated investigate VBN cord-274424-juj71nc5 31 10 by by IN cord-274424-juj71nc5 31 11 the the DT cord-274424-juj71nc5 31 12 introduction introduction NN cord-274424-juj71nc5 31 13 of of IN cord-274424-juj71nc5 31 14 a a DT cord-274424-juj71nc5 31 15 C c NN cord-274424-juj71nc5 31 16 - - HYPH cord-274424-juj71nc5 31 17 terminal terminal NN cord-274424-juj71nc5 31 18 gene gene NN cord-274424-juj71nc5 31 19 deletion deletion NN cord-274424-juj71nc5 31 20 downstream downstream RB cord-274424-juj71nc5 31 21 of of IN cord-274424-juj71nc5 31 22 the the DT cord-274424-juj71nc5 31 23 heptad heptad NN cord-274424-juj71nc5 31 24 repeat repeat NN cord-274424-juj71nc5 31 25 sequence sequence NN cord-274424-juj71nc5 31 26 . . . cord-274424-juj71nc5 32 1 TGEV TGEV NNP cord-274424-juj71nc5 32 2 , , , cord-274424-juj71nc5 32 3 strain strain VB cord-274424-juj71nc5 32 4 FS772/70 FS772/70 NNP cord-274424-juj71nc5 32 5 , , , cord-274424-juj71nc5 32 6 was be VBD cord-274424-juj71nc5 32 7 cultivated cultivate VBN cord-274424-juj71nc5 32 8 in in IN cord-274424-juj71nc5 32 9 a a DT cord-274424-juj71nc5 32 10 porcine porcine JJ cord-274424-juj71nc5 32 11 continuous continuous JJ cord-274424-juj71nc5 32 12 cell cell NN cord-274424-juj71nc5 32 13 line line NN cord-274424-juj71nc5 32 14 ( ( -LRB- cord-274424-juj71nc5 32 15 LLC LLC NNP cord-274424-juj71nc5 32 16 - - HYPH cord-274424-juj71nc5 32 17 PKl PKl NNP cord-274424-juj71nc5 32 18 ) ) -RRB- cord-274424-juj71nc5 32 19 maintained maintain VBD cord-274424-juj71nc5 32 20 with with IN cord-274424-juj71nc5 32 21 medium medium NN cord-274424-juj71nc5 32 22 containing contain VBG cord-274424-juj71nc5 32 23 10 10 CD cord-274424-juj71nc5 32 24 pg pg NN cord-274424-juj71nc5 32 25 ml- ml- NN cord-274424-juj71nc5 32 26 ' ' `` cord-274424-juj71nc5 32 27 trypsin trypsin NN cord-274424-juj71nc5 32 28 ( ( -LRB- cord-274424-juj71nc5 32 29 Hofmann Hofmann NNP cord-274424-juj71nc5 32 30 and and CC cord-274424-juj71nc5 32 31 Wyler Wyler NNP cord-274424-juj71nc5 32 32 , , , cord-274424-juj71nc5 32 33 1988 1988 CD cord-274424-juj71nc5 32 34 ) ) -RRB- cord-274424-juj71nc5 32 35 . . . cord-274424-juj71nc5 33 1 CV-1 CV-1 NNP cord-274424-juj71nc5 33 2 and and CC cord-274424-juj71nc5 33 3 human human JJ cord-274424-juj71nc5 33 4 143 143 CD cord-274424-juj71nc5 33 5 thymidine thymidine NN cord-274424-juj71nc5 33 6 kinase kinase NN cord-274424-juj71nc5 34 1 negative negative JJ cord-274424-juj71nc5 34 2 ( ( -LRB- cord-274424-juj71nc5 34 3 HTK- HTK- NNP cord-274424-juj71nc5 34 4 ) ) -RRB- cord-274424-juj71nc5 34 5 cells cell NNS cord-274424-juj71nc5 34 6 were be VBD cord-274424-juj71nc5 34 7 grown grow VBN cord-274424-juj71nc5 34 8 in in IN cord-274424-juj71nc5 34 9 Eagles Eagles NNP cord-274424-juj71nc5 34 10 MEM MEM NNP cord-274424-juj71nc5 34 11 medium medium NN cord-274424-juj71nc5 34 12 ( ( -LRB- cord-274424-juj71nc5 34 13 Flow Flow NNP cord-274424-juj71nc5 34 14 Labs Labs NNP cord-274424-juj71nc5 34 15 ) ) -RRB- cord-274424-juj71nc5 34 16 containing contain VBG cord-274424-juj71nc5 34 17 10 10 CD cord-274424-juj71nc5 34 18 % % NN cord-274424-juj71nc5 34 19 heat heat NN cord-274424-juj71nc5 34 20 - - HYPH cord-274424-juj71nc5 34 21 inactivated inactivate VBN cord-274424-juj71nc5 34 22 fetal fetal JJ cord-274424-juj71nc5 34 23 calf calf NN cord-274424-juj71nc5 34 24 serum serum NN cord-274424-juj71nc5 34 25 . . . cord-274424-juj71nc5 35 1 Transfections transfection NNS cord-274424-juj71nc5 35 2 were be VBD cord-274424-juj71nc5 35 3 performed perform VBN cord-274424-juj71nc5 35 4 on on IN cord-274424-juj71nc5 35 5 subconfluent subconfluent NN cord-274424-juj71nc5 35 6 monolayers monolayer NNS cord-274424-juj71nc5 35 7 of of IN cord-274424-juj71nc5 35 8 CV-1 CV-1 NNP cord-274424-juj71nc5 35 9 cells cell NNS cord-274424-juj71nc5 35 10 previously previously RB cord-274424-juj71nc5 35 11 infected infect VBN cord-274424-juj71nc5 35 12 with with IN cord-274424-juj71nc5 35 13 wild wild JJ cord-274424-juj71nc5 35 14 - - HYPH cord-274424-juj71nc5 35 15 type type NN cord-274424-juj71nc5 35 16 vaccinia vaccinia NN cord-274424-juj71nc5 35 17 virus virus NN cord-274424-juj71nc5 35 18 ( ( -LRB- cord-274424-juj71nc5 35 19 VV VV NNP cord-274424-juj71nc5 35 20 ) ) -RRB- cord-274424-juj71nc5 36 1 ( ( -LRB- cord-274424-juj71nc5 36 2 WR WR NNP cord-274424-juj71nc5 36 3 strain strain NN cord-274424-juj71nc5 36 4 ) ) -RRB- cord-274424-juj71nc5 36 5 using use VBG cord-274424-juj71nc5 36 6 plasmid plasmid NN cord-274424-juj71nc5 36 7 DNA dna NN cord-274424-juj71nc5 36 8 calcium calcium NN cord-274424-juj71nc5 36 9 phosphate phosphate NN cord-274424-juj71nc5 36 10 precipitates precipitate VBZ cord-274424-juj71nc5 37 1 ( ( -LRB- cord-274424-juj71nc5 37 2 Mackett Mackett NNP cord-274424-juj71nc5 37 3 et et NNP cord-274424-juj71nc5 37 4 a/. a/. NN cord-274424-juj71nc5 37 5 , , , cord-274424-juj71nc5 37 6 1985 1985 CD cord-274424-juj71nc5 37 7 ) ) -RRB- cord-274424-juj71nc5 37 8 . . . cord-274424-juj71nc5 38 1 Recombinant recombinant JJ cord-274424-juj71nc5 38 2 vaccinia vaccinia NN cord-274424-juj71nc5 38 3 viruses virus NNS cord-274424-juj71nc5 38 4 were be VBD cord-274424-juj71nc5 38 5 cultivated cultivate VBN cord-274424-juj71nc5 38 6 in in IN cord-274424-juj71nc5 38 7 HTK HTK NNP cord-274424-juj71nc5 38 8 - - HYPH cord-274424-juj71nc5 38 9 cells cell NNS cord-274424-juj71nc5 38 10 in in IN cord-274424-juj71nc5 38 11 the the DT cord-274424-juj71nc5 38 12 presence presence NN cord-274424-juj71nc5 38 13 of of IN cord-274424-juj71nc5 39 1 25 25 CD cord-274424-juj71nc5 39 2 pg pg NN cord-274424-juj71nc5 39 3 ml- ml- NN cord-274424-juj71nc5 39 4 ' ' `` cord-274424-juj71nc5 39 5 5-bromodeoxyuridine 5-bromodeoxyuridine CD cord-274424-juj71nc5 39 6 ( ( -LRB- cord-274424-juj71nc5 39 7 BUdR BUdR NNP cord-274424-juj71nc5 39 8 ; ; : cord-274424-juj71nc5 39 9 Sigma Sigma NNP cord-274424-juj71nc5 39 10 ) ) -RRB- cord-274424-juj71nc5 39 11 as as IN cord-274424-juj71nc5 39 12 described describe VBN cord-274424-juj71nc5 39 13 by by IN cord-274424-juj71nc5 39 14 Mackett Mackett NNP cord-274424-juj71nc5 39 15 et et FW cord-274424-juj71nc5 39 16 al al NNP cord-274424-juj71nc5 39 17 . . . cord-274424-juj71nc5 40 1 ( ( -LRB- cord-274424-juj71nc5 40 2 1985 1985 CD cord-274424-juj71nc5 40 3 ) ) -RRB- cord-274424-juj71nc5 40 4 . . . cord-274424-juj71nc5 41 1 of of IN cord-274424-juj71nc5 41 2 the the DT cord-274424-juj71nc5 41 3 TGEV TGEV NNP cord-274424-juj71nc5 41 4 S S NNP cord-274424-juj71nc5 41 5 and and CC cord-274424-juj71nc5 41 6 SA SA NNP cord-274424-juj71nc5 41 7 genes gene NNS cord-274424-juj71nc5 41 8 from from IN cord-274424-juj71nc5 41 9 cDNA cdna NN cord-274424-juj71nc5 41 10 Cloning clone VBG cord-274424-juj71nc5 41 11 procedures procedure NNS cord-274424-juj71nc5 41 12 were be VBD cord-274424-juj71nc5 41 13 as as IN cord-274424-juj71nc5 41 14 described describe VBN cord-274424-juj71nc5 41 15 by by IN cord-274424-juj71nc5 41 16 Maniatis Maniatis NNP cord-274424-juj71nc5 41 17 et et NNP cord-274424-juj71nc5 41 18 a/. a/. NNP cord-274424-juj71nc5 41 19 ( ( -LRB- cord-274424-juj71nc5 41 20 1982 1982 CD cord-274424-juj71nc5 41 21 ) ) -RRB- cord-274424-juj71nc5 41 22 . . . cord-274424-juj71nc5 42 1 Enzymes enzyme NNS cord-274424-juj71nc5 42 2 were be VBD cord-274424-juj71nc5 42 3 used use VBN cord-274424-juj71nc5 42 4 according accord VBG cord-274424-juj71nc5 42 5 to to IN cord-274424-juj71nc5 42 6 manufacturers manufacturer NNS cord-274424-juj71nc5 42 7 ' ' POS cord-274424-juj71nc5 42 8 instructions instruction NNS cord-274424-juj71nc5 42 9 ( ( -LRB- cord-274424-juj71nc5 42 10 New New NNP cord-274424-juj71nc5 42 11 England England NNP cord-274424-juj71nc5 42 12 Biolabs Biolabs NNP cord-274424-juj71nc5 42 13 , , , cord-274424-juj71nc5 42 14 Bethesda Bethesda NNP cord-274424-juj71nc5 42 15 Research Research NNP cord-274424-juj71nc5 42 16 Labs Labs NNP cord-274424-juj71nc5 42 17 ) ) -RRB- cord-274424-juj71nc5 42 18 . . . cord-274424-juj71nc5 43 1 Plasmids plasmid NNS cord-274424-juj71nc5 43 2 pTG47 ptg47 NN cord-274424-juj71nc5 43 3 , , , cord-274424-juj71nc5 43 4 pTG25 ptg25 NN cord-274424-juj71nc5 43 5 , , , cord-274424-juj71nc5 43 6 and and CC cord-274424-juj71nc5 43 7 pTG30 pTG30 NNS cord-274424-juj71nc5 43 8 were be VBD cord-274424-juj71nc5 43 9 used use VBN cord-274424-juj71nc5 43 10 to to TO cord-274424-juj71nc5 43 11 reconstruct reconstruct VB cord-274424-juj71nc5 43 12 a a DT cord-274424-juj71nc5 43 13 DNA dna NN cord-274424-juj71nc5 43 14 copy copy NN cord-274424-juj71nc5 43 15 of of IN cord-274424-juj71nc5 43 16 the the DT cord-274424-juj71nc5 43 17 TGEV TGEV NNP cord-274424-juj71nc5 43 18 S S NNP cord-274424-juj71nc5 43 19 gene gene NN cord-274424-juj71nc5 43 20 ; ; : cord-274424-juj71nc5 43 21 the the DT cord-274424-juj71nc5 43 22 procedure procedure NN cord-274424-juj71nc5 43 23 is be VBZ cord-274424-juj71nc5 43 24 outlined outline VBN cord-274424-juj71nc5 43 25 in in IN cord-274424-juj71nc5 43 26 Fig Fig NNP cord-274424-juj71nc5 43 27 . . . cord-274424-juj71nc5 44 1 1 1 LS cord-274424-juj71nc5 44 2 . . . cord-274424-juj71nc5 45 1 Essentially essentially RB cord-274424-juj71nc5 45 2 BarnHI BarnHI NNP cord-274424-juj71nc5 45 3 linkers linker NNS cord-274424-juj71nc5 45 4 ( ( -LRB- cord-274424-juj71nc5 45 5 pCGGATCCG pcggatccg FW cord-274424-juj71nc5 45 6 , , , cord-274424-juj71nc5 45 7 No no NN cord-274424-juj71nc5 45 8 . . NN cord-274424-juj71nc5 45 9 1021 1021 CD cord-274424-juj71nc5 45 10 Biolabs Biolabs NNP cord-274424-juj71nc5 45 11 ) ) -RRB- cord-274424-juj71nc5 45 12 were be VBD cord-274424-juj71nc5 45 13 added add VBN cord-274424-juj71nc5 45 14 to to IN cord-274424-juj71nc5 45 15 the the DT cord-274424-juj71nc5 45 16 Hoal Hoal NNP cord-274424-juj71nc5 45 17 site site NN cord-274424-juj71nc5 45 18 known know VBN cord-274424-juj71nc5 45 19 to to TO cord-274424-juj71nc5 45 20 be be VB cord-274424-juj71nc5 45 21 11 11 CD cord-274424-juj71nc5 45 22 bp bp NNP cord-274424-juj71nc5 45 23 upstream upstream RB cord-274424-juj71nc5 45 24 of of IN cord-274424-juj71nc5 45 25 the the DT cord-274424-juj71nc5 45 26 S S NNP cord-274424-juj71nc5 45 27 gene gene NN cord-274424-juj71nc5 45 28 initiation initiation NN cord-274424-juj71nc5 45 29 codon codon NNP cord-274424-juj71nc5 45 30 ) ) -RRB- cord-274424-juj71nc5 45 31 and and CC cord-274424-juj71nc5 45 32 the the DT cord-274424-juj71nc5 45 33 1.29 1.29 CD cord-274424-juj71nc5 45 34 kb kb NNP cord-274424-juj71nc5 45 35 HindIll HindIll NNP cord-274424-juj71nc5 45 36 fragment fragment NN cord-274424-juj71nc5 45 37 was be VBD cord-274424-juj71nc5 45 38 cloned clone VBN cord-274424-juj71nc5 45 39 into into IN cord-274424-juj71nc5 45 40 pUC9 pUC9 NNP cord-274424-juj71nc5 45 41 and and CC cord-274424-juj71nc5 45 42 excised excise VBD cord-274424-juj71nc5 45 43 as as IN cord-274424-juj71nc5 45 44 a a DT cord-274424-juj71nc5 45 45 0.85-kb 0.85-kb CD cord-274424-juj71nc5 45 46 Xbal Xbal NNP cord-274424-juj71nc5 45 47 - - HYPH cord-274424-juj71nc5 45 48 BarnHI BarnHI NNP cord-274424-juj71nc5 45 49 fragment fragment NN cord-274424-juj71nc5 45 50 . . . cord-274424-juj71nc5 46 1 The the DT cord-274424-juj71nc5 46 2 BarnHI BarnHI NNP cord-274424-juj71nc5 46 3 - - HYPH cord-274424-juj71nc5 46 4 Sty1 sty1 NN cord-274424-juj71nc5 46 5 fragment fragment NN cord-274424-juj71nc5 46 6 derived derive VBN cord-274424-juj71nc5 46 7 from from IN cord-274424-juj71nc5 46 8 pTG47 ptg47 NN cord-274424-juj71nc5 46 9 , , , cord-274424-juj71nc5 46 10 the the DT cord-274424-juj71nc5 46 11 Styl Styl NNP cord-274424-juj71nc5 46 12 - - HYPH cord-274424-juj71nc5 46 13 Kpnl Kpnl NNP cord-274424-juj71nc5 46 14 fragment fragment NN cord-274424-juj71nc5 46 15 derived derive VBN cord-274424-juj71nc5 46 16 from from IN cord-274424-juj71nc5 46 17 pTG25 pTG25 NNP cord-274424-juj71nc5 46 18 , , , cord-274424-juj71nc5 46 19 the the DT cord-274424-juj71nc5 46 20 Kpnl Kpnl NNP cord-274424-juj71nc5 46 21 - - HYPH cord-274424-juj71nc5 46 22 Xbal Xbal NNP cord-274424-juj71nc5 46 23 fragment fragment NN cord-274424-juj71nc5 46 24 derived derive VBN cord-274424-juj71nc5 46 25 from from IN cord-274424-juj71nc5 46 26 pTG30 pTG30 NNS cord-274424-juj71nc5 46 27 , , , cord-274424-juj71nc5 46 28 the the DT cord-274424-juj71nc5 46 29 0.85-kb 0.85-kb CD cord-274424-juj71nc5 46 30 Xbal Xbal NNP cord-274424-juj71nc5 46 31 - - HYPH cord-274424-juj71nc5 46 32 BarnHI BarnHI NNP cord-274424-juj71nc5 46 33 fragment fragment NN cord-274424-juj71nc5 46 34 , , , cord-274424-juj71nc5 46 35 and and CC cord-274424-juj71nc5 46 36 BarnHI BarnHI NNP cord-274424-juj71nc5 46 37 dephosphorylated dephosphorylate VBD cord-274424-juj71nc5 46 38 pBR322 pbr322 PRP cord-274424-juj71nc5 46 39 were be VBD cord-274424-juj71nc5 46 40 mixed mix VBN cord-274424-juj71nc5 46 41 , , , cord-274424-juj71nc5 46 42 ligated ligate VBN cord-274424-juj71nc5 46 43 in in IN cord-274424-juj71nc5 46 44 a a DT cord-274424-juj71nc5 46 45 five five CD cord-274424-juj71nc5 46 46 - - HYPH cord-274424-juj71nc5 46 47 way way NN cord-274424-juj71nc5 46 48 reaction reaction NN cord-274424-juj71nc5 46 49 mixture mixture NN cord-274424-juj71nc5 46 50 , , , cord-274424-juj71nc5 46 51 and and CC cord-274424-juj71nc5 46 52 transformed transform VBN cord-274424-juj71nc5 46 53 into into IN cord-274424-juj71nc5 46 54 DHl DHl NNP cord-274424-juj71nc5 46 55 Escherichia Escherichia NNP cord-274424-juj71nc5 46 56 co co NN cord-274424-juj71nc5 46 57 / / SYM cord-274424-juj71nc5 46 58 i i PRP cord-274424-juj71nc5 46 59 cells cell NNS cord-274424-juj71nc5 46 60 . . . cord-274424-juj71nc5 47 1 As as IN cord-274424-juj71nc5 47 2 a a DT cord-274424-juj71nc5 47 3 result result NN cord-274424-juj71nc5 47 4 of of IN cord-274424-juj71nc5 47 5 the the DT cord-274424-juj71nc5 47 6 different different JJ cord-274424-juj71nc5 47 7 cohesive cohesive JJ cord-274424-juj71nc5 47 8 ends end VBZ cord-274424-juj71nc5 47 9 an an DT cord-274424-juj71nc5 47 10 insert insert NN cord-274424-juj71nc5 47 11 of of IN cord-274424-juj71nc5 47 12 4.6 4.6 CD cord-274424-juj71nc5 47 13 kb kb NNP cord-274424-juj71nc5 47 14 would would MD cord-274424-juj71nc5 47 15 correspond correspond VB cord-274424-juj71nc5 47 16 only only RB cord-274424-juj71nc5 47 17 to to IN cord-274424-juj71nc5 47 18 the the DT cord-274424-juj71nc5 47 19 complete complete JJ cord-274424-juj71nc5 47 20 S S NNP cord-274424-juj71nc5 47 21 gene gene NN cord-274424-juj71nc5 47 22 . . . cord-274424-juj71nc5 48 1 An an DT cord-274424-juj71nc5 48 2 ApRTcS ApRTcS NNP cord-274424-juj71nc5 48 3 transformant transformant NN cord-274424-juj71nc5 48 4 was be VBD cord-274424-juj71nc5 48 5 found find VBN cord-274424-juj71nc5 48 6 to to TO cord-274424-juj71nc5 48 7 contain contain VB cord-274424-juj71nc5 48 8 a a DT cord-274424-juj71nc5 48 9 plasmid plasmid NN cord-274424-juj71nc5 48 10 , , , cord-274424-juj71nc5 48 11 pPBP1 ppbp1 UH cord-274424-juj71nc5 48 12 , , , cord-274424-juj71nc5 48 13 with with IN cord-274424-juj71nc5 48 14 a a DT cord-274424-juj71nc5 48 15 4.6-kbp 4.6-kbp CD cord-274424-juj71nc5 48 16 insert insert NN cord-274424-juj71nc5 48 17 in in IN cord-274424-juj71nc5 48 18 pBR322 pbr322 CD cord-274424-juj71nc5 48 19 , , , cord-274424-juj71nc5 48 20 corresponding correspond VBG cord-274424-juj71nc5 48 21 to to IN cord-274424-juj71nc5 48 22 the the DT cord-274424-juj71nc5 48 23 TGEV TGEV NNP cord-274424-juj71nc5 48 24 S S NNP cord-274424-juj71nc5 48 25 BarnHI BarnHI NNP cord-274424-juj71nc5 48 26 gene gene NN cord-274424-juj71nc5 48 27 cassette cassette NN cord-274424-juj71nc5 48 28 . . . cord-274424-juj71nc5 49 1 The the DT cord-274424-juj71nc5 49 2 reconstructed reconstructed JJ cord-274424-juj71nc5 49 3 gene gene NN cord-274424-juj71nc5 49 4 was be VBD cord-274424-juj71nc5 49 5 verified verify VBN cord-274424-juj71nc5 49 6 by by IN cord-274424-juj71nc5 49 7 sequencing sequence VBG cord-274424-juj71nc5 49 8 the the DT cord-274424-juj71nc5 49 9 junction junction NN cord-274424-juj71nc5 49 10 regions region NNS cord-274424-juj71nc5 49 11 . . . cord-274424-juj71nc5 50 1 The the DT cord-274424-juj71nc5 50 2 TGEV TGEV NNP cord-274424-juj71nc5 50 3 cDNA cdna NN cord-274424-juj71nc5 50 4 S s NN cord-274424-juj71nc5 50 5 gene gene NN cord-274424-juj71nc5 50 6 was be VBD cord-274424-juj71nc5 50 7 subcloned subclone VBN cord-274424-juj71nc5 50 8 from from IN cord-274424-juj71nc5 50 9 pPBP1 pPBP1 NNS cord-274424-juj71nc5 50 10 and and CC cord-274424-juj71nc5 50 11 inserted insert VBD cord-274424-juj71nc5 50 12 into into IN cord-274424-juj71nc5 50 13 the the DT cord-274424-juj71nc5 50 14 BarnHI BarnHI NNP cord-274424-juj71nc5 50 15 cloning clone VBG cord-274424-juj71nc5 50 16 site site NN cord-274424-juj71nc5 50 17 of of IN cord-274424-juj71nc5 50 18 the the DT cord-274424-juj71nc5 50 19 VV VV NNP cord-274424-juj71nc5 50 20 plasmid plasmid NN cord-274424-juj71nc5 50 21 insertion insertion NN cord-274424-juj71nc5 50 22 vector vector NN cord-274424-juj71nc5 50 23 pGS20 pGS20 NNP cord-274424-juj71nc5 51 1 ( ( -LRB- cord-274424-juj71nc5 51 2 Mackett Mackett NNP cord-274424-juj71nc5 51 3 et et FW cord-274424-juj71nc5 51 4 al al NNP cord-274424-juj71nc5 51 5 . . NNP cord-274424-juj71nc5 51 6 , , , cord-274424-juj71nc5 51 7 1984 1984 CD cord-274424-juj71nc5 51 8 ) ) -RRB- cord-274424-juj71nc5 51 9 downstream downstream NN cord-274424-juj71nc5 51 10 of of IN cord-274424-juj71nc5 51 11 the the DT cord-274424-juj71nc5 51 12 W W NNP cord-274424-juj71nc5 51 13 early early JJ cord-274424-juj71nc5 51 14 / / SYM cord-274424-juj71nc5 51 15 late late JJ cord-274424-juj71nc5 51 16 P p NN cord-274424-juj71nc5 51 17 7,5 7,5 CD cord-274424-juj71nc5 51 18 K K NNP cord-274424-juj71nc5 51 19 promoter promoter NN cord-274424-juj71nc5 51 20 . . . cord-274424-juj71nc5 52 1 The the DT cord-274424-juj71nc5 52 2 recombinant recombinant JJ cord-274424-juj71nc5 52 3 plasmid plasmid NN cord-274424-juj71nc5 52 4 , , , cord-274424-juj71nc5 52 5 pGSP-1 pgsp-1 CD cord-274424-juj71nc5 52 6 , , , cord-274424-juj71nc5 52 7 containing contain VBG cord-274424-juj71nc5 52 8 a a DT cord-274424-juj71nc5 52 9 correctly correctly RB cord-274424-juj71nc5 52 10 orientated orientated JJ cord-274424-juj71nc5 52 11 S S NNP cord-274424-juj71nc5 52 12 gene gene NN cord-274424-juj71nc5 52 13 , , , cord-274424-juj71nc5 52 14 was be VBD cord-274424-juj71nc5 52 15 identified identify VBN cord-274424-juj71nc5 52 16 by by IN cord-274424-juj71nc5 52 17 restriction restriction NN cord-274424-juj71nc5 52 18 endonuclease endonuclease NN cord-274424-juj71nc5 52 19 digestion digestion NN cord-274424-juj71nc5 52 20 with with IN cord-274424-juj71nc5 52 21 Sall Sall NNP cord-274424-juj71nc5 52 22 . . . cord-274424-juj71nc5 53 1 The the DT cord-274424-juj71nc5 53 2 S S NNP cord-274424-juj71nc5 53 3 protein protein NN cord-274424-juj71nc5 53 4 with with IN cord-274424-juj71nc5 53 5 a a DT cord-274424-juj71nc5 53 6 C c NN cord-274424-juj71nc5 53 7 - - HYPH cord-274424-juj71nc5 53 8 terminal terminal NN cord-274424-juj71nc5 53 9 deletion deletion NN cord-274424-juj71nc5 53 10 was be VBD cord-274424-juj71nc5 53 11 generated generate VBN cord-274424-juj71nc5 53 12 by by IN cord-274424-juj71nc5 53 13 cloning clone VBG cord-274424-juj71nc5 53 14 a a DT cord-274424-juj71nc5 53 15 3.7-kbp 3.7-kbp CD cord-274424-juj71nc5 53 16 SalI SalI NNP cord-274424-juj71nc5 53 17 fragment fragment NN cord-274424-juj71nc5 53 18 from from IN cord-274424-juj71nc5 53 19 pGSP-1 pGSP-1 VBG cord-274424-juj71nc5 53 20 into into IN cord-274424-juj71nc5 53 21 the the DT cord-274424-juj71nc5 53 22 SalI SalI NNP cord-274424-juj71nc5 53 23 site site NN cord-274424-juj71nc5 53 24 of of IN cord-274424-juj71nc5 53 25 pUCl2 pucl2 CD cord-274424-juj71nc5 53 26 . . . cord-274424-juj71nc5 54 1 Restriction restriction NN cord-274424-juj71nc5 54 2 endonuclease endonuclease NN cord-274424-juj71nc5 54 3 digestion digestion NN cord-274424-juj71nc5 54 4 of of IN cord-274424-juj71nc5 54 5 recombinant recombinant JJ cord-274424-juj71nc5 54 6 plasmids plasmid NNS cord-274424-juj71nc5 54 7 produced produce VBD cord-274424-juj71nc5 55 1 either either CC cord-274424-juj71nc5 55 2 a a DT cord-274424-juj71nc5 55 3 0.25-or 0.25-or CD cord-274424-juj71nc5 55 4 3.45-kbp 3.45-kbp CD cord-274424-juj71nc5 55 5 BarnHI BarnHI NNP cord-274424-juj71nc5 55 6 fragment fragment NN cord-274424-juj71nc5 55 7 and and CC cord-274424-juj71nc5 55 8 the the DT cord-274424-juj71nc5 55 9 latter latter JJ cord-274424-juj71nc5 55 10 was be VBD cord-274424-juj71nc5 55 11 recloned reclone VBN cord-274424-juj71nc5 55 12 back back RB cord-274424-juj71nc5 55 13 into into IN cord-274424-juj71nc5 55 14 BarnHI BarnHI NNP cord-274424-juj71nc5 55 15 - - HYPH cord-274424-juj71nc5 55 16 digested digest VBN cord-274424-juj71nc5 55 17 pGS20 pGS20 NNS cord-274424-juj71nc5 55 18 . . . cord-274424-juj71nc5 56 1 The the DT cord-274424-juj71nc5 56 2 recombinant recombinant JJ cord-274424-juj71nc5 56 3 plasmid plasmid NN cord-274424-juj71nc5 56 4 insertion insertion NN cord-274424-juj71nc5 56 5 vector vector NN cord-274424-juj71nc5 56 6 pGSPA-38 pgspa-38 NN cord-274424-juj71nc5 56 7 was be VBD cord-274424-juj71nc5 56 8 found find VBN cord-274424-juj71nc5 56 9 to to TO cord-274424-juj71nc5 56 10 contain contain VB cord-274424-juj71nc5 56 11 a a DT cord-274424-juj71nc5 56 12 correctly correctly RB cord-274424-juj71nc5 56 13 orientated orientate VBN cord-274424-juj71nc5 56 14 SA SA NNP cord-274424-juj71nc5 56 15 gene gene NN cord-274424-juj71nc5 56 16 by by IN cord-274424-juj71nc5 57 1 Sryl sryl NN cord-274424-juj71nc5 57 2 restriction restriction NN cord-274424-juj71nc5 57 3 endonuclease endonuclease NN cord-274424-juj71nc5 57 4 digestion digestion NN cord-274424-juj71nc5 57 5 of of IN cord-274424-juj71nc5 57 6 plasmid plasmid NN cord-274424-juj71nc5 57 7 DNA dna NN cord-274424-juj71nc5 57 8 . . . cord-274424-juj71nc5 58 1 A a DT cord-274424-juj71nc5 58 2 synthetic synthetic JJ cord-274424-juj71nc5 58 3 oligonucleotide oligonucleotide JJ cord-274424-juj71nc5 58 4 primer primer NN cord-274424-juj71nc5 58 5 5'-GTGTGCGGCTAC 5'-gtgtgcggctac CD cord-274424-juj71nc5 58 6 - - HYPH cord-274424-juj71nc5 58 7 TATAACTA-3 TATAACTA-3 NNP cord-274424-juj71nc5 58 8 ' ' '' cord-274424-juj71nc5 58 9 , , , cord-274424-juj71nc5 58 10 that that WDT cord-274424-juj71nc5 58 11 binds bind VBZ cord-274424-juj71nc5 58 12 to to IN cord-274424-juj71nc5 58 13 the the DT cord-274424-juj71nc5 58 14 complementary complementary JJ cord-274424-juj71nc5 58 15 DNA dna NN cord-274424-juj71nc5 58 16 strand strand NN cord-274424-juj71nc5 58 17 43 43 CD cord-274424-juj71nc5 58 18 bp bp NNP cord-274424-juj71nc5 58 19 downstream downstream NN cord-274424-juj71nc5 58 20 of of IN cord-274424-juj71nc5 58 21 the the DT cord-274424-juj71nc5 58 22 BarnHI BarnHI NNP cord-274424-juj71nc5 58 23 cloning clone VBG cord-274424-juj71nc5 58 24 site site NN cord-274424-juj71nc5 58 25 in in IN cord-274424-juj71nc5 58 26 pGS20 pGS20 NNP cord-274424-juj71nc5 58 27 , , , cord-274424-juj71nc5 58 28 verified verify VBD cord-274424-juj71nc5 58 29 the the DT cord-274424-juj71nc5 58 30 position position NN cord-274424-juj71nc5 58 31 of of IN cord-274424-juj71nc5 58 32 the the DT cord-274424-juj71nc5 58 33 SA SA NNP cord-274424-juj71nc5 58 34 protein protein NN cord-274424-juj71nc5 58 35 stop stop VB cord-274424-juj71nc5 58 36 codon codon NN cord-274424-juj71nc5 58 37 in in IN cord-274424-juj71nc5 58 38 pGSPA-38 pgspa-38 NN cord-274424-juj71nc5 58 39 by by IN cord-274424-juj71nc5 58 40 sequencing sequence VBG cord-274424-juj71nc5 58 41 ( ( -LRB- cord-274424-juj71nc5 58 42 Fig Fig NNP cord-274424-juj71nc5 58 43 . . . cord-274424-juj71nc5 58 44 2A 2a CD cord-274424-juj71nc5 58 45 ) ) -RRB- cord-274424-juj71nc5 58 46 . . . cord-274424-juj71nc5 59 1 The the DT cord-274424-juj71nc5 59 2 rVVs rvvs NN cord-274424-juj71nc5 59 3 , , , cord-274424-juj71nc5 59 4 vTS-1 vTS-1 NNP cord-274424-juj71nc5 59 5 and and CC cord-274424-juj71nc5 59 6 vTSA-1 vTSA-1 NNP cord-274424-juj71nc5 59 7 , , , cord-274424-juj71nc5 59 8 were be VBD cord-274424-juj71nc5 59 9 generated generate VBN cord-274424-juj71nc5 59 10 with with IN cord-274424-juj71nc5 59 11 pGSP-1 pgsp-1 CD cord-274424-juj71nc5 59 12 and and CC cord-274424-juj71nc5 59 13 pGSPA-38 pgspa-38 NN cord-274424-juj71nc5 59 14 using use VBG cord-274424-juj71nc5 59 15 methods method NNS cord-274424-juj71nc5 59 16 described describe VBN cord-274424-juj71nc5 59 17 by by IN cord-274424-juj71nc5 59 18 Mackett Mackett NNP cord-274424-juj71nc5 59 19 et et FW cord-274424-juj71nc5 59 20 al al NNP cord-274424-juj71nc5 59 21 . . . cord-274424-juj71nc5 60 1 ( ( -LRB- cord-274424-juj71nc5 60 2 1984 1984 CD cord-274424-juj71nc5 60 3 ) ) -RRB- cord-274424-juj71nc5 60 4 . . . cord-274424-juj71nc5 61 1 Thymidine thymidine NN cord-274424-juj71nc5 61 2 kinase kinase VBD cord-274424-juj71nc5 61 3 negative negative JJ cord-274424-juj71nc5 61 4 viruses virus NNS cord-274424-juj71nc5 61 5 were be VBD cord-274424-juj71nc5 61 6 screened screen VBN cord-274424-juj71nc5 61 7 by by IN cord-274424-juj71nc5 61 8 DNA dna NN cord-274424-juj71nc5 61 9 dot dot NN cord-274424-juj71nc5 61 10 blot blot NN cord-274424-juj71nc5 61 11 and and CC cord-274424-juj71nc5 61 12 DNA dna NN cord-274424-juj71nc5 61 13 from from IN cord-274424-juj71nc5 61 14 vTS-1 vTS-1 NNP cord-274424-juj71nc5 61 15 and and CC cord-274424-juj71nc5 61 16 vTSA-1 vTSA-1 , cord-274424-juj71nc5 61 17 infected infected JJ cord-274424-juj71nc5 61 18 cells cell NNS cord-274424-juj71nc5 61 19 was be VBD cord-274424-juj71nc5 61 20 analyzed analyze VBN cord-274424-juj71nc5 61 21 by by IN cord-274424-juj71nc5 61 22 Southern southern JJ cord-274424-juj71nc5 61 23 blot blot NN cord-274424-juj71nc5 61 24 using use VBG cord-274424-juj71nc5 61 25 a a DT cord-274424-juj71nc5 61 26 [ [ -LRB- cord-274424-juj71nc5 61 27 32P]-labeled 32P]-labeled NNP cord-274424-juj71nc5 61 28 S S NNP cord-274424-juj71nc5 61 29 gene gene NN cord-274424-juj71nc5 61 30 cDNA cdna NN cord-274424-juj71nc5 61 31 probe probe NN cord-274424-juj71nc5 61 32 as as IN cord-274424-juj71nc5 61 33 described describe VBN cord-274424-juj71nc5 61 34 in in IN cord-274424-juj71nc5 61 35 . . . cord-274424-juj71nc5 62 1 Groups group NNS cord-274424-juj71nc5 62 2 of of IN cord-274424-juj71nc5 62 3 three three CD cord-274424-juj71nc5 62 4 female female JJ cord-274424-juj71nc5 62 5 Balb Balb NNP cord-274424-juj71nc5 62 6 / / SYM cord-274424-juj71nc5 62 7 C C NNP cord-274424-juj71nc5 62 8 mice mouse NNS cord-274424-juj71nc5 62 9 were be VBD cord-274424-juj71nc5 62 10 immunized immunize VBN cord-274424-juj71nc5 62 11 by by IN cord-274424-juj71nc5 62 12 intraperitoneal intraperitoneal JJ cord-274424-juj71nc5 62 13 ( ( -LRB- cord-274424-juj71nc5 62 14 ip ip NN cord-274424-juj71nc5 62 15 ) ) -RRB- cord-274424-juj71nc5 62 16 inoculation inoculation NN cord-274424-juj71nc5 62 17 with with IN cord-274424-juj71nc5 62 18 l-2 l-2 NNP cord-274424-juj71nc5 62 19 X X NNP cord-274424-juj71nc5 62 20 1 1 CD cord-274424-juj71nc5 62 21 O5 o5 CD cord-274424-juj71nc5 62 22 PFU/ pfu/ JJ cord-274424-juj71nc5 62 23 mouse mouse NN cord-274424-juj71nc5 62 24 of of IN cord-274424-juj71nc5 62 25 partially partially RB cord-274424-juj71nc5 62 26 purified purify VBN cord-274424-juj71nc5 62 27 recombinant recombinant JJ cord-274424-juj71nc5 62 28 or or CC cord-274424-juj71nc5 62 29 wildtype wildtype JJ cord-274424-juj71nc5 62 30 VV VV NNP cord-274424-juj71nc5 62 31 as as IN cord-274424-juj71nc5 62 32 described describe VBN cord-274424-juj71nc5 62 33 by by IN cord-274424-juj71nc5 62 34 . . . cord-274424-juj71nc5 63 1 After after IN cord-274424-juj71nc5 63 2 4 4 CD cord-274424-juj71nc5 63 3 weeks week NNS cord-274424-juj71nc5 63 4 , , , cord-274424-juj71nc5 63 5 one one CD cord-274424-juj71nc5 63 6 animal animal NN cord-274424-juj71nc5 63 7 from from IN cord-274424-juj71nc5 63 8 each each DT cord-274424-juj71nc5 63 9 group group NN cord-274424-juj71nc5 63 10 was be VBD cord-274424-juj71nc5 63 11 sacrificed sacrifice VBN cord-274424-juj71nc5 63 12 to to TO cord-274424-juj71nc5 63 13 obtain obtain VB cord-274424-juj71nc5 63 14 convalescent convalescent JJ cord-274424-juj71nc5 63 15 serum serum NN cord-274424-juj71nc5 63 16 , , , cord-274424-juj71nc5 63 17 the the DT cord-274424-juj71nc5 63 18 remaining remain VBG cord-274424-juj71nc5 63 19 animals animal NNS cord-274424-juj71nc5 63 20 were be VBD cord-274424-juj71nc5 63 21 hyperimmunized hyperimmunize VBN cord-274424-juj71nc5 63 22 with with IN cord-274424-juj71nc5 63 23 homologous homologous JJ cord-274424-juj71nc5 63 24 virus virus NN cord-274424-juj71nc5 63 25 by by IN cord-274424-juj71nc5 63 26 ip ip NN cord-274424-juj71nc5 63 27 inoculation inoculation NN cord-274424-juj71nc5 63 28 and and CC cord-274424-juj71nc5 63 29 bled bleed VBD cord-274424-juj71nc5 63 30 after after IN cord-274424-juj71nc5 63 31 a a DT cord-274424-juj71nc5 63 32 further further JJ cord-274424-juj71nc5 63 33 4 4 CD cord-274424-juj71nc5 63 34 weeks week NNS cord-274424-juj71nc5 63 35 . . . cord-274424-juj71nc5 64 1 Mice mouse NNS cord-274424-juj71nc5 64 2 initially initially RB cord-274424-juj71nc5 64 3 inoculated inoculate VBD cord-274424-juj71nc5 64 4 with with IN cord-274424-juj71nc5 64 5 either either CC cord-274424-juj71nc5 64 6 phosphate phosphate NN cord-274424-juj71nc5 64 7 - - HYPH cord-274424-juj71nc5 64 8 buffered buffer VBN cord-274424-juj71nc5 64 9 saline saline NN cord-274424-juj71nc5 64 10 ( ( -LRB- cord-274424-juj71nc5 64 11 PBS PBS NNP cord-274424-juj71nc5 64 12 ) ) -RRB- cord-274424-juj71nc5 64 13 or or CC cord-274424-juj71nc5 64 14 WR WR NNP cord-274424-juj71nc5 64 15 strain strain NN cord-274424-juj71nc5 64 16 VV VV NNP cord-274424-juj71nc5 64 17 were be VBD cord-274424-juj71nc5 64 18 inoculated inoculate VBN cord-274424-juj71nc5 64 19 after after IN cord-274424-juj71nc5 64 20 4 4 CD cord-274424-juj71nc5 64 21 weeks week NNS cord-274424-juj71nc5 64 22 with with IN cord-274424-juj71nc5 64 23 a a DT cord-274424-juj71nc5 64 24 dose dose NN cord-274424-juj71nc5 64 25 of of IN cord-274424-juj71nc5 64 26 vTS-1 vTS-1 NNP cord-274424-juj71nc5 64 27 to to TO cord-274424-juj71nc5 64 28 establish establish VB cord-274424-juj71nc5 64 29 if if IN cord-274424-juj71nc5 64 30 age age NN cord-274424-juj71nc5 64 31 or or CC cord-274424-juj71nc5 64 32 a a DT cord-274424-juj71nc5 64 33 previous previous JJ cord-274424-juj71nc5 64 34 W w NN cord-274424-juj71nc5 64 35 infection infection NN cord-274424-juj71nc5 64 36 affected affect VBD cord-274424-juj71nc5 64 37 the the DT cord-274424-juj71nc5 64 38 stimulation stimulation NN cord-274424-juj71nc5 64 39 of of IN cord-274424-juj71nc5 64 40 TGEV TGEV NNP cord-274424-juj71nc5 64 41 neutralizing neutralize VBG cord-274424-juj71nc5 64 42 antibody antibody NN cord-274424-juj71nc5 64 43 . . . cord-274424-juj71nc5 65 1 TGEV TGEV NNP cord-274424-juj71nc5 65 2 neutralizing neutralize VBG cord-274424-juj71nc5 65 3 antibody antibody NN cord-274424-juj71nc5 65 4 was be VBD cord-274424-juj71nc5 65 5 assessed assess VBN cord-274424-juj71nc5 65 6 by by IN cord-274424-juj71nc5 65 7 plaque plaque NN cord-274424-juj71nc5 65 8 reduction reduction NN cord-274424-juj71nc5 65 9 assay assay NN cord-274424-juj71nc5 65 10 as as IN cord-274424-juj71nc5 65 11 described describe VBN cord-274424-juj71nc5 65 12 by by IN cord-274424-juj71nc5 65 13 Garwes Garwes NNP cord-274424-juj71nc5 65 14 et et NNP cord-274424-juj71nc5 65 15 a/. a/. NN cord-274424-juj71nc5 65 16 ( ( -LRB- cord-274424-juj71nc5 65 17 1987 1987 CD cord-274424-juj71nc5 65 18 ) ) -RRB- cord-274424-juj71nc5 65 19 . . . cord-274424-juj71nc5 66 1 Viral viral JJ cord-274424-juj71nc5 66 2 proteins protein NNS cord-274424-juj71nc5 66 3 were be VBD cord-274424-juj71nc5 66 4 routinely routinely RB cord-274424-juj71nc5 66 5 radiolabeled radiolabele VBN cord-274424-juj71nc5 66 6 by by IN cord-274424-juj71nc5 66 7 incubating incubate VBG cord-274424-juj71nc5 66 8 TGEV TGEV NNP cord-274424-juj71nc5 66 9 - - HYPH cord-274424-juj71nc5 66 10 infected infect VBN cord-274424-juj71nc5 66 11 LLC LLC NNP cord-274424-juj71nc5 66 12 - - HYPH cord-274424-juj71nc5 66 13 PKl PKl NNP cord-274424-juj71nc5 66 14 cells cell NNS cord-274424-juj71nc5 66 15 or or CC cord-274424-juj71nc5 66 16 rVV rvv RB cord-274424-juj71nc5 66 17 - - HYPH cord-274424-juj71nc5 66 18 infected infect VBN cord-274424-juj71nc5 66 19 HTK HTK NNP cord-274424-juj71nc5 66 20 - - HYPH cord-274424-juj71nc5 66 21 cells cell NNS cord-274424-juj71nc5 66 22 , , , cord-274424-juj71nc5 66 23 at at IN cord-274424-juj71nc5 66 24 8 8 CD cord-274424-juj71nc5 66 25 hr hr NN cord-274424-juj71nc5 66 26 p.i p.i NNP cord-274424-juj71nc5 66 27 . . NNP cord-274424-juj71nc5 66 28 or or CC cord-274424-juj71nc5 66 29 17 17 CD cord-274424-juj71nc5 66 30 hr hr NN cord-274424-juj71nc5 66 31 p.i p.i NNP cord-274424-juj71nc5 66 32 . . NNP cord-274424-juj71nc5 66 33 , , , cord-274424-juj71nc5 66 34 respectively respectively RB cord-274424-juj71nc5 66 35 , , , cord-274424-juj71nc5 66 36 in in IN cord-274424-juj71nc5 66 37 methionine methionine NN cord-274424-juj71nc5 66 38 - - HYPH cord-274424-juj71nc5 66 39 free free JJ cord-274424-juj71nc5 66 40 medium medium NN cord-274424-juj71nc5 66 41 for for IN cord-274424-juj71nc5 66 42 1 1 CD cord-274424-juj71nc5 66 43 hr hr NN cord-274424-juj71nc5 66 44 and and CC cord-274424-juj71nc5 66 45 then then RB cord-274424-juj71nc5 66 46 in in IN cord-274424-juj71nc5 66 47 the the DT cord-274424-juj71nc5 66 48 presence presence NN cord-274424-juj71nc5 66 49 of of IN cord-274424-juj71nc5 66 50 50-l 50-l CD cord-274424-juj71nc5 66 51 00 00 NFP cord-274424-juj71nc5 67 1 & & CC cord-274424-juj71nc5 67 2 i i PRP cord-274424-juj71nc5 67 3 ml- ml- VBD cord-274424-juj71nc5 67 4 ' ' '' cord-274424-juj71nc5 68 1 L-[35S]methionine L-[35S]methionine NNP cord-274424-juj71nc5 69 1 ( ( -LRB- cord-274424-juj71nc5 69 2 Amersham Amersham NNP cord-274424-juj71nc5 69 3 International International NNP cord-274424-juj71nc5 69 4 ; ; : cord-274424-juj71nc5 69 5 see see VB cord-274424-juj71nc5 69 6 figure figure NN cord-274424-juj71nc5 69 7 legends legend NNS cord-274424-juj71nc5 69 8 for for IN cord-274424-juj71nc5 69 9 further further JJ cord-274424-juj71nc5 69 10 details detail NNS cord-274424-juj71nc5 69 11 ) ) -RRB- cord-274424-juj71nc5 69 12 . . . cord-274424-juj71nc5 70 1 After after IN cord-274424-juj71nc5 70 2 pulse pulse NN cord-274424-juj71nc5 70 3 labeling labeling NN cord-274424-juj71nc5 70 4 , , , cord-274424-juj71nc5 70 5 cells cell NNS cord-274424-juj71nc5 70 6 were be VBD cord-274424-juj71nc5 70 7 washed wash VBN cord-274424-juj71nc5 70 8 in in IN cord-274424-juj71nc5 70 9 PBS PBS NNP cord-274424-juj71nc5 70 10 ( ( -LRB- cord-274424-juj71nc5 70 11 10 10 CD cord-274424-juj71nc5 70 12 mM mm CD cord-274424-juj71nc5 70 13 potassium potassium NN cord-274424-juj71nc5 70 14 phosphate phosphate NN cord-274424-juj71nc5 70 15 , , , cord-274424-juj71nc5 70 16 150 150 CD cord-274424-juj71nc5 70 17 m m NN cord-274424-juj71nc5 70 18 / / SYM cord-274424-juj71nc5 70 19 l l NN cord-274424-juj71nc5 70 20 / / SYM cord-274424-juj71nc5 70 21 l l NNP cord-274424-juj71nc5 70 22 NaCI NaCI NNP cord-274424-juj71nc5 70 23 , , , cord-274424-juj71nc5 70 24 pH pH NNP cord-274424-juj71nc5 70 25 7.2 7.2 CD cord-274424-juj71nc5 70 26 ) ) -RRB- cord-274424-juj71nc5 70 27 and and CC cord-274424-juj71nc5 70 28 chased chase VBN cord-274424-juj71nc5 70 29 in in IN cord-274424-juj71nc5 70 30 medium medium NN cord-274424-juj71nc5 70 31 supplemented supplement VBN cord-274424-juj71nc5 70 32 with with IN cord-274424-juj71nc5 70 33 2 2 CD cord-274424-juj71nc5 70 34 mM mm CD cord-274424-juj71nc5 70 35 L l NN cord-274424-juj71nc5 70 36 - - HYPH cord-274424-juj71nc5 70 37 methionine methionine NN cord-274424-juj71nc5 70 38 . . . cord-274424-juj71nc5 71 1 Cells cell NNS cord-274424-juj71nc5 71 2 were be VBD cord-274424-juj71nc5 71 3 washed wash VBN cord-274424-juj71nc5 71 4 in in IN cord-274424-juj71nc5 71 5 PBS PBS NNP cord-274424-juj71nc5 71 6 before before IN cord-274424-juj71nc5 71 7 treating treat VBG cord-274424-juj71nc5 71 8 with with IN cord-274424-juj71nc5 71 9 TEST test NN cord-274424-juj71nc5 71 10 lysis lysis NN cord-274424-juj71nc5 71 11 buffer buffer NN cord-274424-juj71nc5 71 12 ( ( -LRB- cord-274424-juj71nc5 71 13 20 20 CD cord-274424-juj71nc5 71 14 mM mM NNP cord-274424-juj71nc5 71 15 Tris Tris NNP cord-274424-juj71nc5 71 16 - - HYPH cord-274424-juj71nc5 71 17 HCI HCI NNP cord-274424-juj71nc5 71 18 , , , cord-274424-juj71nc5 71 19 pH pH NNP cord-274424-juj71nc5 71 20 7.6 7.6 CD cord-274424-juj71nc5 71 21 , , , cord-274424-juj71nc5 71 22 2 2 CD cord-274424-juj71nc5 71 23 mM mm CD cord-274424-juj71nc5 71 24 EDTA EDTA NNP cord-274424-juj71nc5 71 25 , , , cord-274424-juj71nc5 71 26 100 100 CD cord-274424-juj71nc5 71 27 mM mm CD cord-274424-juj71nc5 71 28 NaCI naci NN cord-274424-juj71nc5 71 29 , , , cord-274424-juj71nc5 71 30 1 1 CD cord-274424-juj71nc5 71 31 % % NN cord-274424-juj71nc5 71 32 Triton Triton NNP cord-274424-juj71nc5 71 33 X X NNP cord-274424-juj71nc5 71 34 - - HYPH cord-274424-juj71nc5 71 35 l l NN cord-274424-juj71nc5 71 36 00 00 CD cord-274424-juj71nc5 71 37 , , , cord-274424-juj71nc5 71 38 0.1 0.1 CD cord-274424-juj71nc5 71 39 % % NN cord-274424-juj71nc5 71 40 aprotinin aprotinin NNS cord-274424-juj71nc5 71 41 ) ) -RRB- cord-274424-juj71nc5 71 42 . . . cord-274424-juj71nc5 72 1 Nuclei nuclei NN cord-274424-juj71nc5 72 2 and and CC cord-274424-juj71nc5 72 3 ceil ceil NN cord-274424-juj71nc5 72 4 debris debris NN cord-274424-juj71nc5 72 5 were be VBD cord-274424-juj71nc5 72 6 pelleted pellete VBN cord-274424-juj71nc5 72 7 from from IN cord-274424-juj71nc5 72 8 infected infected JJ cord-274424-juj71nc5 72 9 cell cell NN cord-274424-juj71nc5 72 10 lysates lysate NNS cord-274424-juj71nc5 72 11 by by IN cord-274424-juj71nc5 72 12 centrifugation centrifugation NN cord-274424-juj71nc5 72 13 at at IN cord-274424-juj71nc5 72 14 100,000 100,000 CD cord-274424-juj71nc5 72 15 g g NN cord-274424-juj71nc5 72 16 using use VBG cord-274424-juj71nc5 72 17 a a DT cord-274424-juj71nc5 72 18 Beckman Beckman NNP cord-274424-juj71nc5 72 19 TLA-100 TLA-100 NNP cord-274424-juj71nc5 72 20 ultracentrifuge ultracentrifuge JJ cord-274424-juj71nc5 72 21 . . . cord-274424-juj71nc5 73 1 Tissue tissue NN cord-274424-juj71nc5 73 2 culture culture NN cord-274424-juj71nc5 73 3 medium medium NN cord-274424-juj71nc5 73 4 was be VBD cord-274424-juj71nc5 73 5 clarified clarify VBN cord-274424-juj71nc5 73 6 by by IN cord-274424-juj71nc5 73 7 low low JJ cord-274424-juj71nc5 73 8 speed speed NN cord-274424-juj71nc5 73 9 centrifugation centrifugation NN cord-274424-juj71nc5 73 10 . . . cord-274424-juj71nc5 74 1 Immunoprecipitations immunoprecipitation NNS cord-274424-juj71nc5 74 2 were be VBD cord-274424-juj71nc5 74 3 performed perform VBN cord-274424-juj71nc5 74 4 by by IN cord-274424-juj71nc5 74 5 mixing mix VBG cord-274424-juj71nc5 74 6 1 1 CD cord-274424-juj71nc5 74 7 vol vol NN cord-274424-juj71nc5 74 8 of of IN cord-274424-juj71nc5 74 9 cell cell NN cord-274424-juj71nc5 74 10 lysate lysate NN cord-274424-juj71nc5 74 11 with with IN cord-274424-juj71nc5 74 12 l l NNP cord-274424-juj71nc5 74 13 / / SYM cord-274424-juj71nc5 74 14 l l NNP cord-274424-juj71nc5 74 15 00th 00th NNP cord-274424-juj71nc5 74 16 vol vol NNP cord-274424-juj71nc5 74 17 of of IN cord-274424-juj71nc5 74 18 porcine porcine JJ cord-274424-juj71nc5 74 19 TGEV TGEV NNP cord-274424-juj71nc5 74 20 hyperimmune hyperimmune NNP cord-274424-juj71nc5 74 21 antiserum antiserum NN cord-274424-juj71nc5 74 22 at at IN cord-274424-juj71nc5 74 23 4 4 CD cord-274424-juj71nc5 74 24 ' ' '' cord-274424-juj71nc5 74 25 for for IN cord-274424-juj71nc5 74 26 1 1 CD cord-274424-juj71nc5 74 27 hr hr NN cord-274424-juj71nc5 74 28 and and CC cord-274424-juj71nc5 74 29 the the DT cord-274424-juj71nc5 74 30 resulting result VBG cord-274424-juj71nc5 74 31 immune immune JJ cord-274424-juj71nc5 74 32 complexes complex NNS cord-274424-juj71nc5 74 33 were be VBD cord-274424-juj71nc5 74 34 incubated incubate VBN cord-274424-juj71nc5 74 35 overnight overnight RB cord-274424-juj71nc5 74 36 with with IN cord-274424-juj71nc5 74 37 formalinfixed formalinfixed NNP cord-274424-juj71nc5 74 38 Staphy Staphy NNP cord-274424-juj71nc5 74 39 / / SYM cord-274424-juj71nc5 74 40 ococcus ococcus NNP cord-274424-juj71nc5 74 41 aureus aureus NN cord-274424-juj71nc5 74 42 cells cell NNS cord-274424-juj71nc5 74 43 ( ( -LRB- cord-274424-juj71nc5 74 44 SAC sac NN cord-274424-juj71nc5 74 45 ; ; : cord-274424-juj71nc5 74 46 BRL BRL NNP cord-274424-juj71nc5 74 47 ) ) -RRB- cord-274424-juj71nc5 74 48 previously previously RB cord-274424-juj71nc5 74 49 washed wash VBD cord-274424-juj71nc5 74 50 three three CD cord-274424-juj71nc5 74 51 times time NNS cord-274424-juj71nc5 74 52 in in IN cord-274424-juj71nc5 74 53 TEST test NN cord-274424-juj71nc5 74 54 buffer buffer NN cord-274424-juj71nc5 74 55 . . . cord-274424-juj71nc5 75 1 Immune immune JJ cord-274424-juj71nc5 75 2 complexes complex NNS cord-274424-juj71nc5 75 3 were be VBD cord-274424-juj71nc5 75 4 washed wash VBN cord-274424-juj71nc5 75 5 three three CD cord-274424-juj71nc5 75 6 times time NNS cord-274424-juj71nc5 75 7 with with IN cord-274424-juj71nc5 75 8 TEST test NN cord-274424-juj71nc5 75 9 buffer buffer NN cord-274424-juj71nc5 75 10 and and CC cord-274424-juj71nc5 75 11 resuspended resuspend VBD cord-274424-juj71nc5 75 12 in in IN cord-274424-juj71nc5 75 13 Laemmli Laemmli NNP cord-274424-juj71nc5 75 14 sample sample NN cord-274424-juj71nc5 75 15 buffer buffer NN cord-274424-juj71nc5 75 16 , , , cord-274424-juj71nc5 75 17 and and CC cord-274424-juj71nc5 75 18 proteins protein NNS cord-274424-juj71nc5 75 19 separated separate VBD cord-274424-juj71nc5 75 20 on on IN cord-274424-juj71nc5 75 21 6 6 CD cord-274424-juj71nc5 75 22 % % NN cord-274424-juj71nc5 75 23 SDS sds NN cord-274424-juj71nc5 75 24 - - HYPH cord-274424-juj71nc5 75 25 polyacrylamide polyacrylamide NN cord-274424-juj71nc5 75 26 gels gel NNS cord-274424-juj71nc5 75 27 ( ( -LRB- cord-274424-juj71nc5 75 28 Laemmli Laemmli NNP cord-274424-juj71nc5 75 29 et et NNP cord-274424-juj71nc5 75 30 a/. a/. NN cord-274424-juj71nc5 75 31 , , , cord-274424-juj71nc5 75 32 1970 1970 CD cord-274424-juj71nc5 75 33 ) ) -RRB- cord-274424-juj71nc5 75 34 . . . cord-274424-juj71nc5 76 1 14C 14c NN cord-274424-juj71nc5 76 2 - - HYPH cord-274424-juj71nc5 76 3 methylated methylate VBN cord-274424-juj71nc5 76 4 proteins protein NNS cord-274424-juj71nc5 76 5 ( ( -LRB- cord-274424-juj71nc5 76 6 Amersham Amersham NNP cord-274424-juj71nc5 76 7 , , , cord-274424-juj71nc5 76 8 code code NN cord-274424-juj71nc5 76 9 CFA.626 CFA.626 NNS cord-274424-juj71nc5 76 10 ) ) -RRB- cord-274424-juj71nc5 76 11 were be VBD cord-274424-juj71nc5 76 12 routinely routinely RB cord-274424-juj71nc5 76 13 used use VBN cord-274424-juj71nc5 76 14 as as IN cord-274424-juj71nc5 76 15 molecular molecular JJ cord-274424-juj71nc5 76 16 weight weight NN cord-274424-juj71nc5 76 17 markers marker NNS cord-274424-juj71nc5 76 18 . . . cord-274424-juj71nc5 77 1 Proteins protein NNS cord-274424-juj71nc5 77 2 were be VBD cord-274424-juj71nc5 77 3 detected detect VBN cord-274424-juj71nc5 77 4 by by IN cord-274424-juj71nc5 77 5 fluorography fluorography NN cord-274424-juj71nc5 77 6 after after IN cord-274424-juj71nc5 77 7 immersing immerse VBG cord-274424-juj71nc5 77 8 gels gel NNS cord-274424-juj71nc5 77 9 in in IN cord-274424-juj71nc5 77 10 0.8 0.8 CD cord-274424-juj71nc5 77 11 M m CD cord-274424-juj71nc5 77 12 sodium sodium NN cord-274424-juj71nc5 77 13 salicylate salicylate NN cord-274424-juj71nc5 77 14 for for IN cord-274424-juj71nc5 77 15 30 30 CD cord-274424-juj71nc5 77 16 min min NN cord-274424-juj71nc5 77 17 . . . cord-274424-juj71nc5 78 1 Pelleted pellete VBN cord-274424-juj71nc5 78 2 immune immune JJ cord-274424-juj71nc5 78 3 complexes complex NNS cord-274424-juj71nc5 78 4 were be VBD cord-274424-juj71nc5 78 5 washed wash VBN cord-274424-juj71nc5 78 6 as as IN cord-274424-juj71nc5 78 7 described describe VBN cord-274424-juj71nc5 78 8 above above RB cord-274424-juj71nc5 78 9 , , , cord-274424-juj71nc5 78 10 resuspended resuspend VBD cord-274424-juj71nc5 78 11 in in IN cord-274424-juj71nc5 78 12 40 40 CD cord-274424-juj71nc5 78 13 ~I50 ~I50 NNP cord-274424-juj71nc5 78 14 mll mll NNP cord-274424-juj71nc5 78 15 / / SYM cord-274424-juj71nc5 78 16 l l NNP cord-274424-juj71nc5 78 17 Tris Tris NNP cord-274424-juj71nc5 78 18 - - HYPH cord-274424-juj71nc5 78 19 HCI HCI NNP cord-274424-juj71nc5 78 20 , , , cord-274424-juj71nc5 78 21 pH pH NNP cord-274424-juj71nc5 78 22 6.8 6.8 CD cord-274424-juj71nc5 78 23 , , , cord-274424-juj71nc5 78 24 containing contain VBG cord-274424-juj71nc5 78 25 0.25 0.25 CD cord-274424-juj71nc5 78 26 % % NN cord-274424-juj71nc5 78 27 SDS SDS NNP cord-274424-juj71nc5 78 28 , , , cord-274424-juj71nc5 78 29 and and CC cord-274424-juj71nc5 78 30 boiled boil VBD cord-274424-juj71nc5 78 31 for for IN cord-274424-juj71nc5 78 32 2 2 CD cord-274424-juj71nc5 78 33 min min NN cord-274424-juj71nc5 78 34 . . . cord-274424-juj71nc5 79 1 The the DT cord-274424-juj71nc5 79 2 solubilized solubilized JJ cord-274424-juj71nc5 79 3 proteins protein NNS cord-274424-juj71nc5 79 4 were be VBD cord-274424-juj71nc5 79 5 incubated incubate VBN cord-274424-juj71nc5 79 6 in in IN cord-274424-juj71nc5 79 7 the the DT cord-274424-juj71nc5 79 8 presence presence NN cord-274424-juj71nc5 79 9 or or CC cord-274424-juj71nc5 79 10 absence absence NN cord-274424-juj71nc5 79 11 of of IN cord-274424-juj71nc5 79 12 1 1 CD cord-274424-juj71nc5 79 13 mU mU NNP cord-274424-juj71nc5 79 14 Endo endo NN cord-274424-juj71nc5 79 15 H H NNP cord-274424-juj71nc5 79 16 ( ( -LRB- cord-274424-juj71nc5 79 17 Boehringer Boehringer NNP cord-274424-juj71nc5 79 18 - - HYPH cord-274424-juj71nc5 79 19 Mannheim Mannheim NNP cord-274424-juj71nc5 79 20 ) ) -RRB- cord-274424-juj71nc5 79 21 in in IN cord-274424-juj71nc5 79 22 90 90 CD cord-274424-juj71nc5 79 23 mMsodium mMsodium NNP cord-274424-juj71nc5 79 24 citrate citrate NN cord-274424-juj71nc5 79 25 , , , cord-274424-juj71nc5 79 26 pH pH NNP cord-274424-juj71nc5 79 27 5.5 5.5 CD cord-274424-juj71nc5 79 28 , , , cord-274424-juj71nc5 79 29 at at IN cord-274424-juj71nc5 79 30 37 37 CD cord-274424-juj71nc5 79 31 " " `` cord-274424-juj71nc5 79 32 for for IN cord-274424-juj71nc5 79 33 18 18 CD cord-274424-juj71nc5 79 34 hr hr NN cord-274424-juj71nc5 79 35 . . . cord-274424-juj71nc5 80 1 The the DT cord-274424-juj71nc5 80 2 proteins protein NNS cord-274424-juj71nc5 80 3 were be VBD cord-274424-juj71nc5 80 4 analyzed analyze VBN cord-274424-juj71nc5 80 5 on on IN cord-274424-juj71nc5 80 6 6 6 CD cord-274424-juj71nc5 80 7 % % NN cord-274424-juj71nc5 80 8 polyacrylamide polyacrylamide NN cord-274424-juj71nc5 80 9 gels gel NNS cord-274424-juj71nc5 80 10 and and CC cord-274424-juj71nc5 80 11 detected detect VBN cord-274424-juj71nc5 80 12 by by IN cord-274424-juj71nc5 80 13 fluorography fluorography NNP cord-274424-juj71nc5 80 14 . . . cord-274424-juj71nc5 81 1 Glass glass NN cord-274424-juj71nc5 81 2 coverslips coverslip NNS cord-274424-juj71nc5 81 3 with with IN cord-274424-juj71nc5 81 4 subconfluent subconfluent NN cord-274424-juj71nc5 81 5 monolayer monolayer NN cord-274424-juj71nc5 81 6 cultures culture NNS cord-274424-juj71nc5 81 7 of of IN cord-274424-juj71nc5 81 8 HTK HTK NNP cord-274424-juj71nc5 81 9 - - HYPH cord-274424-juj71nc5 81 10 or or CC cord-274424-juj71nc5 81 11 LLC LLC NNP cord-274424-juj71nc5 81 12 - - HYPH cord-274424-juj71nc5 81 13 PKl PKl NNP cord-274424-juj71nc5 81 14 cells cell NNS cord-274424-juj71nc5 81 15 were be VBD cord-274424-juj71nc5 81 16 infected infect VBN cord-274424-juj71nc5 81 17 with with IN cord-274424-juj71nc5 81 18 TGEV TGEV NNP cord-274424-juj71nc5 81 19 and and CC cord-274424-juj71nc5 81 20 wild wild JJ cord-274424-juj71nc5 81 21 - - HYPH cord-274424-juj71nc5 81 22 type type NN cord-274424-juj71nc5 81 23 or or CC cord-274424-juj71nc5 81 24 rVVs rvvs FW cord-274424-juj71nc5 81 25 . . . cord-274424-juj71nc5 82 1 At at IN cord-274424-juj71nc5 82 2 20 20 CD cord-274424-juj71nc5 82 3 hr hr NN cord-274424-juj71nc5 82 4 p.i p.i NNP cord-274424-juj71nc5 82 5 . . NNP cord-274424-juj71nc5 82 6 cells cell NNS cord-274424-juj71nc5 82 7 were be VBD cord-274424-juj71nc5 82 8 either either RB cord-274424-juj71nc5 82 9 fixed fix VBN cord-274424-juj71nc5 82 10 with with IN cord-274424-juj71nc5 82 11 cold cold JJ cord-274424-juj71nc5 82 12 80 80 CD cord-274424-juj71nc5 82 13 % % NN cord-274424-juj71nc5 82 14 acetone acetone NN cord-274424-juj71nc5 82 15 and and CC cord-274424-juj71nc5 82 16 air air NN cord-274424-juj71nc5 82 17 dried dry VBN cord-274424-juj71nc5 82 18 or or CC cord-274424-juj71nc5 82 19 washed wash VBN cord-274424-juj71nc5 82 20 in in IN cord-274424-juj71nc5 82 21 cold cold JJ cord-274424-juj71nc5 82 22 PBS PBS NNP cord-274424-juj71nc5 82 23 and and CC cord-274424-juj71nc5 82 24 maintained maintain VBD cord-274424-juj71nc5 82 25 at at IN cord-274424-juj71nc5 82 26 4 4 CD cord-274424-juj71nc5 82 27 " " `` cord-274424-juj71nc5 82 28 for for IN cord-274424-juj71nc5 82 29 surface surface NN cord-274424-juj71nc5 82 30 staining staining NN cord-274424-juj71nc5 82 31 . . . cord-274424-juj71nc5 83 1 Cells cell NNS cord-274424-juj71nc5 83 2 were be VBD cord-274424-juj71nc5 83 3 incubated incubate VBN cord-274424-juj71nc5 83 4 for for IN cord-274424-juj71nc5 83 5 1 1 CD cord-274424-juj71nc5 83 6 hr hr NN cord-274424-juj71nc5 83 7 with with IN cord-274424-juj71nc5 83 8 porcine porcine JJ cord-274424-juj71nc5 83 9 TGEV TGEV NNP cord-274424-juj71nc5 83 10 hyperimmune hyperimmune NNP cord-274424-juj71nc5 83 11 antiserum antiserum NN cord-274424-juj71nc5 83 12 diluted dilute VBD cord-274424-juj71nc5 83 13 1 1 CD cord-274424-juj71nc5 83 14 : : : cord-274424-juj71nc5 84 1 lOO lOO NNP cord-274424-juj71nc5 84 2 in in IN cord-274424-juj71nc5 84 3 PBS PBS NNP cord-274424-juj71nc5 84 4 and and CC cord-274424-juj71nc5 84 5 then then RB cord-274424-juj71nc5 84 6 extensively extensively RB cord-274424-juj71nc5 84 7 washed wash VBD cord-274424-juj71nc5 84 8 with with IN cord-274424-juj71nc5 84 9 PBS PBS NNP cord-274424-juj71nc5 84 10 . . . cord-274424-juj71nc5 85 1 Cells cell NNS cord-274424-juj71nc5 85 2 were be VBD cord-274424-juj71nc5 85 3 then then RB cord-274424-juj71nc5 85 4 incubated incubate VBN cord-274424-juj71nc5 85 5 for for IN cord-274424-juj71nc5 85 6 30 30 CD cord-274424-juj71nc5 85 7 min min NN cord-274424-juj71nc5 85 8 with with IN cord-274424-juj71nc5 85 9 fluorescein fluorescein NN cord-274424-juj71nc5 85 10 isothiocyanate isothiocyanate NN cord-274424-juj71nc5 85 11 - - HYPH cord-274424-juj71nc5 85 12 conjugated conjugate VBN cord-274424-juj71nc5 85 13 rabbit rabbit NN cord-274424-juj71nc5 85 14 anti anti JJ cord-274424-juj71nc5 85 15 - - JJ cord-274424-juj71nc5 85 16 pig pig NN cord-274424-juj71nc5 86 1 immunoglobulin immunoglobulin NN cord-274424-juj71nc5 86 2 G G NNP cord-274424-juj71nc5 86 3 ( ( -LRB- cord-274424-juj71nc5 86 4 Nor nor CC cord-274424-juj71nc5 86 5 - - HYPH cord-274424-juj71nc5 86 6 die die VB cord-274424-juj71nc5 86 7 Immunology Immunology NNP cord-274424-juj71nc5 86 8 ) ) -RRB- cord-274424-juj71nc5 86 9 diluted dilute VBD cord-274424-juj71nc5 86 10 1:40 1:40 CD cord-274424-juj71nc5 86 11 in in IN cord-274424-juj71nc5 86 12 PBS PBS NNP cord-274424-juj71nc5 86 13 . . . cord-274424-juj71nc5 87 1 Coverslips coverslip NNS cord-274424-juj71nc5 87 2 were be VBD cord-274424-juj71nc5 87 3 washed wash VBN cord-274424-juj71nc5 87 4 , , , cord-274424-juj71nc5 87 5 air air NN cord-274424-juj71nc5 87 6 dried dry VBN cord-274424-juj71nc5 87 7 , , , cord-274424-juj71nc5 87 8 and and CC cord-274424-juj71nc5 87 9 mounted mount VBD cord-274424-juj71nc5 87 10 on on IN cord-274424-juj71nc5 87 11 glass glass NN cord-274424-juj71nc5 87 12 slides slide NNS cord-274424-juj71nc5 87 13 with with IN cord-274424-juj71nc5 87 14 80 80 CD cord-274424-juj71nc5 87 15 % % NN cord-274424-juj71nc5 87 16 glycerol glycerol NN cord-274424-juj71nc5 87 17 . . . cord-274424-juj71nc5 88 1 Fluorescent fluorescent NN cord-274424-juj71nc5 88 2 cells cell NNS cord-274424-juj71nc5 88 3 were be VBD cord-274424-juj71nc5 88 4 observed observe VBN cord-274424-juj71nc5 88 5 and and CC cord-274424-juj71nc5 88 6 photographed photograph VBN cord-274424-juj71nc5 88 7 with with IN cord-274424-juj71nc5 88 8 a a DT cord-274424-juj71nc5 88 9 Leitz Leitz NNP cord-274424-juj71nc5 88 10 Wetzlar Wetzlar NNP cord-274424-juj71nc5 88 11 UV uv NN cord-274424-juj71nc5 88 12 microscope microscope NN cord-274424-juj71nc5 88 13 . . . cord-274424-juj71nc5 89 1 A a DT cord-274424-juj71nc5 89 2 4.6-kbp 4.6-kbp CD cord-274424-juj71nc5 89 3 BarnHI BarnHI NNP cord-274424-juj71nc5 89 4 S S NNP cord-274424-juj71nc5 89 5 gene gene NN cord-274424-juj71nc5 89 6 cassette cassette NN cord-274424-juj71nc5 89 7 was be VBD cord-274424-juj71nc5 89 8 constructed construct VBN cord-274424-juj71nc5 89 9 from from IN cord-274424-juj71nc5 89 10 TGEV TGEV NNP cord-274424-juj71nc5 89 11 FS772/70 FS772/70 NNP cord-274424-juj71nc5 89 12 cDNA cdna NN cord-274424-juj71nc5 89 13 and and CC cord-274424-juj71nc5 89 14 used use VBD cord-274424-juj71nc5 89 15 to to TO cord-274424-juj71nc5 89 16 generate generate VB cord-274424-juj71nc5 89 17 pPBP1 pPBP1 NNP cord-274424-juj71nc5 90 1 ( ( -LRB- cord-274424-juj71nc5 90 2 Fig Fig NNP cord-274424-juj71nc5 90 3 . . NNP cord-274424-juj71nc5 90 4 1 1 CD cord-274424-juj71nc5 90 5 ) ) -RRB- cord-274424-juj71nc5 90 6 . . . cord-274424-juj71nc5 91 1 The the DT cord-274424-juj71nc5 91 2 4350-bp 4350-bp JJ cord-274424-juj71nc5 91 3 S S NNP cord-274424-juj71nc5 91 4 gene gene NN cord-274424-juj71nc5 91 5 was be VBD cord-274424-juj71nc5 91 6 capable capable JJ cord-274424-juj71nc5 91 7 of of IN cord-274424-juj71nc5 91 8 encoding encode VBG cord-274424-juj71nc5 91 9 a a DT cord-274424-juj71nc5 91 10 precursor precursor NN cord-274424-juj71nc5 91 11 polypeptide polypeptide NN cord-274424-juj71nc5 91 12 of of IN cord-274424-juj71nc5 91 13 1449 1449 CD cord-274424-juj71nc5 91 14 amino amino NN cord-274424-juj71nc5 91 15 acids acid NNS cord-274424-juj71nc5 91 16 with with IN cord-274424-juj71nc5 91 17 a a DT cord-274424-juj71nc5 91 18 M M NNP cord-274424-juj71nc5 91 19 , , , cord-274424-juj71nc5 91 20 159,811 159,811 CD cord-274424-juj71nc5 91 21 . . . cord-274424-juj71nc5 92 1 After after IN cord-274424-juj71nc5 92 2 cleavage cleavage NN cord-274424-juj71nc5 92 3 of of IN cord-274424-juj71nc5 92 4 the the DT cord-274424-juj71nc5 92 5 N n CD cord-274424-juj71nc5 92 6 - - HYPH cord-274424-juj71nc5 92 7 terminal terminal NN cord-274424-juj71nc5 92 8 signal signal NN cord-274424-juj71nc5 92 9 peptide peptide NN cord-274424-juj71nc5 92 10 , , , cord-274424-juj71nc5 92 11 shown show VBN cord-274424-juj71nc5 92 12 to to TO cord-274424-juj71nc5 92 13 be be VB cord-274424-juj71nc5 92 14 absent absent JJ cord-274424-juj71nc5 92 15 in in IN cord-274424-juj71nc5 92 16 virion virion NN cord-274424-juj71nc5 92 17 - - HYPH cord-274424-juj71nc5 92 18 associated associate VBN cord-274424-juj71nc5 92 19 Purdue Purdue NNP cord-274424-juj71nc5 92 20 strain strain NN cord-274424-juj71nc5 92 21 S s NN cord-274424-juj71nc5 92 22 protein protein NN cord-274424-juj71nc5 92 23 ( ( -LRB- cord-274424-juj71nc5 92 24 Rasschaert Rasschaert NNP cord-274424-juj71nc5 92 25 and and CC cord-274424-juj71nc5 92 26 Laude Laude NNP cord-274424-juj71nc5 92 27 , , , cord-274424-juj71nc5 92 28 1987 1987 CD cord-274424-juj71nc5 92 29 ) ) -RRB- cord-274424-juj71nc5 93 1 the the DT cord-274424-juj71nc5 93 2 protein protein NN cord-274424-juj71nc5 93 3 would would MD cord-274424-juj71nc5 93 4 consist consist VB cord-274424-juj71nc5 93 5 of of IN cord-274424-juj71nc5 93 6 1433 1433 CD cord-274424-juj71nc5 93 7 amino amino NN cord-274424-juj71nc5 93 8 acid acid NN cord-274424-juj71nc5 93 9 residues residue NNS cord-274424-juj71nc5 93 10 with with IN cord-274424-juj71nc5 93 11 a a DT cord-274424-juj71nc5 93 12 AI ai NN cord-274424-juj71nc5 93 13 , , , cord-274424-juj71nc5 93 14 157,891 157,891 CD cord-274424-juj71nc5 93 15 . . . cord-274424-juj71nc5 94 1 The the DT cord-274424-juj71nc5 94 2 S S NNP cord-274424-juj71nc5 94 3 gene gene NN cord-274424-juj71nc5 94 4 BarnHI BarnHI NNP cord-274424-juj71nc5 94 5 gene gene NN cord-274424-juj71nc5 94 6 cassette cassette NN cord-274424-juj71nc5 94 7 was be VBD cord-274424-juj71nc5 94 8 subcloned subclone VBN cord-274424-juj71nc5 94 9 into into IN cord-274424-juj71nc5 94 10 the the DT cord-274424-juj71nc5 94 11 VV VV NNP cord-274424-juj71nc5 94 12 insetion insetion NN cord-274424-juj71nc5 94 13 vector vector NNP cord-274424-juj71nc5 94 14 pGS20 pGS20 NNP cord-274424-juj71nc5 94 15 to to TO cord-274424-juj71nc5 94 16 give give VB cord-274424-juj71nc5 94 17 pGSP-1 pGSP-1 NNP cord-274424-juj71nc5 94 18 as as IN cord-274424-juj71nc5 94 19 described describe VBN cord-274424-juj71nc5 94 20 under under IN cord-274424-juj71nc5 94 21 Materials material NNS cord-274424-juj71nc5 94 22 and and CC cord-274424-juj71nc5 94 23 Methods method NNS cord-274424-juj71nc5 94 24 . . . cord-274424-juj71nc5 95 1 The the DT cord-274424-juj71nc5 95 2 SA SA NNP cord-274424-juj71nc5 95 3 gene gene NN cord-274424-juj71nc5 95 4 , , , cord-274424-juj71nc5 95 5 contained contain VBN cord-274424-juj71nc5 95 6 in in IN cord-274424-juj71nc5 95 7 pGSPA-38 pGSPA-38 NNP cord-274424-juj71nc5 95 8 , , , cord-274424-juj71nc5 95 9 was be VBD cord-274424-juj71nc5 95 10 3474 3474 CD cord-274424-juj71nc5 95 11 bp bp NNP cord-274424-juj71nc5 95 12 long long NNP cord-274424-juj71nc5 95 13 , , , cord-274424-juj71nc5 95 14 capable capable JJ cord-274424-juj71nc5 95 15 of of IN cord-274424-juj71nc5 95 16 encoding encode VBG cord-274424-juj71nc5 95 17 a a DT cord-274424-juj71nc5 95 18 1157-amino 1157-amino CD cord-274424-juj71nc5 95 19 acid acid NN cord-274424-juj71nc5 95 20 polypeptide polypeptide NN cord-274424-juj71nc5 95 21 , , , cord-274424-juj71nc5 95 22 corresponding correspond VBG cord-274424-juj71nc5 95 23 to to IN cord-274424-juj71nc5 95 24 a a DT cord-274424-juj71nc5 95 25 deletion deletion NN cord-274424-juj71nc5 95 26 of of IN cord-274424-juj71nc5 95 27 292 292 CD cord-274424-juj71nc5 95 28 residues residue NNS cord-274424-juj71nc5 95 29 from from IN cord-274424-juj71nc5 95 30 the the DT cord-274424-juj71nc5 95 31 complete complete JJ cord-274424-juj71nc5 95 32 S S NNP cord-274424-juj71nc5 95 33 protein protein NN cord-274424-juj71nc5 95 34 and and CC cord-274424-juj71nc5 95 35 also also RB cord-274424-juj71nc5 95 36 included include VBD cord-274424-juj71nc5 95 37 eight eight CD cord-274424-juj71nc5 95 38 amino amino NN cord-274424-juj71nc5 95 39 acid acid NN cord-274424-juj71nc5 95 40 residues residue NNS cord-274424-juj71nc5 95 41 derived derive VBN cord-274424-juj71nc5 95 42 from from IN cord-274424-juj71nc5 95 43 the the DT cord-274424-juj71nc5 95 44 pUCl2 pucl2 CD cord-274424-juj71nc5 95 45 polylinker polylinker NN cord-274424-juj71nc5 95 46 and and CC cord-274424-juj71nc5 95 47 pGS20 pGS20 NNP cord-274424-juj71nc5 95 48 sequences sequence NNS cord-274424-juj71nc5 95 49 . . . cord-274424-juj71nc5 96 1 The the DT cord-274424-juj71nc5 96 2 SA SA NNP cord-274424-juj71nc5 96 3 gene gene NN cord-274424-juj71nc5 96 4 terminated terminate VBN cord-274424-juj71nc5 96 5 in in IN cord-274424-juj71nc5 96 6 a a DT cord-274424-juj71nc5 96 7 new new JJ cord-274424-juj71nc5 96 8 TGA TGA NNP cord-274424-juj71nc5 96 9 stop stop NN cord-274424-juj71nc5 96 10 codon codon NN cord-274424-juj71nc5 96 11 contained contain VBN cord-274424-juj71nc5 96 12 within within IN cord-274424-juj71nc5 96 13 the the DT cord-274424-juj71nc5 96 14 VV VV NNP cord-274424-juj71nc5 96 15 TK TK NNP cord-274424-juj71nc5 96 16 sequences sequence NNS cord-274424-juj71nc5 96 17 ( ( -LRB- cord-274424-juj71nc5 96 18 Fig Fig NNP cord-274424-juj71nc5 96 19 . . NNP cord-274424-juj71nc5 96 20 2 2 CD cord-274424-juj71nc5 96 21 ) ) -RRB- cord-274424-juj71nc5 96 22 . . . cord-274424-juj71nc5 97 1 The the DT cord-274424-juj71nc5 97 2 predicted predict VBN cord-274424-juj71nc5 97 3 size size NN cord-274424-juj71nc5 97 4 of of IN cord-274424-juj71nc5 97 5 the the DT cord-274424-juj71nc5 97 6 precursor precursor NN cord-274424-juj71nc5 97 7 SA SA NNP cord-274424-juj71nc5 97 8 polypeptide polypeptide NN cord-274424-juj71nc5 97 9 was be VBD cord-274424-juj71nc5 97 10 M M NNP cord-274424-juj71nc5 97 11 , , , cord-274424-juj71nc5 97 12 126,748 126,748 CD cord-274424-juj71nc5 97 13 , , , cord-274424-juj71nc5 97 14 which which WDT cord-274424-juj71nc5 97 15 when when WRB cord-274424-juj71nc5 97 16 modified modify VBN cord-274424-juj71nc5 97 17 by by IN cord-274424-juj71nc5 97 18 the the DT cord-274424-juj71nc5 97 19 removal removal NN cord-274424-juj71nc5 97 20 of of IN cord-274424-juj71nc5 97 21 the the DT cord-274424-juj71nc5 97 22 16-amino 16-amino CD cord-274424-juj71nc5 97 23 acid acid NN cord-274424-juj71nc5 97 24 N n CD cord-274424-juj71nc5 97 25 - - HYPH cord-274424-juj71nc5 97 26 terminal terminal NN cord-274424-juj71nc5 97 27 signal signal NN cord-274424-juj71nc5 97 28 peptide peptide NN cord-274424-juj71nc5 97 29 was be VBD cord-274424-juj71nc5 97 30 reduced reduce VBN cord-274424-juj71nc5 97 31 to to IN cord-274424-juj71nc5 97 32 M M NNP cord-274424-juj71nc5 97 33 , , , cord-274424-juj71nc5 97 34 124,916 124,916 CD cord-274424-juj71nc5 97 35 . . . cord-274424-juj71nc5 98 1 The the DT cord-274424-juj71nc5 98 2 C c NN cord-274424-juj71nc5 98 3 - - HYPH cord-274424-juj71nc5 98 4 terminal terminal NN cord-274424-juj71nc5 98 5 deletion deletion NN cord-274424-juj71nc5 98 6 also also RB cord-274424-juj71nc5 98 7 resulted result VBD cord-274424-juj71nc5 98 8 in in IN cord-274424-juj71nc5 98 9 removal removal NN cord-274424-juj71nc5 98 10 of of IN cord-274424-juj71nc5 98 11 eight eight CD cord-274424-juj71nc5 98 12 potential potential JJ cord-274424-juj71nc5 98 13 Nlinked Nlinked NNP cord-274424-juj71nc5 98 14 glycosylation glycosylation NN cord-274424-juj71nc5 98 15 sites site NNS cord-274424-juj71nc5 98 16 predicted predict VBN cord-274424-juj71nc5 98 17 for for IN cord-274424-juj71nc5 98 18 the the DT cord-274424-juj71nc5 98 19 S S NNP cord-274424-juj71nc5 98 20 protein protein NN cord-274424-juj71nc5 98 21 . . . cord-274424-juj71nc5 99 1 The the DT cord-274424-juj71nc5 99 2 S S NNP cord-274424-juj71nc5 99 3 gene gene NN cord-274424-juj71nc5 99 4 initiation initiation NN cord-274424-juj71nc5 99 5 codon codon NN cord-274424-juj71nc5 99 6 was be VBD cord-274424-juj71nc5 99 7 54 54 CD cord-274424-juj71nc5 99 8 bp bp NNS cord-274424-juj71nc5 99 9 away away RB cord-274424-juj71nc5 99 10 from from IN cord-274424-juj71nc5 99 11 the the DT cord-274424-juj71nc5 99 12 VV VV NNP cord-274424-juj71nc5 99 13 early early JJ cord-274424-juj71nc5 99 14 promoter promoter NN cord-274424-juj71nc5 99 15 RNA RNA NNP cord-274424-juj71nc5 99 16 start start NN cord-274424-juj71nc5 99 17 site site NN cord-274424-juj71nc5 99 18 for for IN cord-274424-juj71nc5 99 19 both both DT cord-274424-juj71nc5 99 20 pGSP-1 pgsp-1 CD cord-274424-juj71nc5 99 21 and and CC cord-274424-juj71nc5 99 22 pGSPA-38 pgspa-38 JJ cord-274424-juj71nc5 99 23 insertion insertion NN cord-274424-juj71nc5 99 24 vectors vector NNS cord-274424-juj71nc5 99 25 . . . cord-274424-juj71nc5 100 1 The the DT cord-274424-juj71nc5 100 2 TGEV TGEV NNP cord-274424-juj71nc5 100 3 S S NNP cord-274424-juj71nc5 100 4 genes gene NNS cord-274424-juj71nc5 100 5 were be VBD cord-274424-juj71nc5 100 6 inserted insert VBN cord-274424-juj71nc5 100 7 into into IN cord-274424-juj71nc5 100 8 the the DT cord-274424-juj71nc5 100 9 VV VV NNP cord-274424-juj71nc5 100 10 genome genome NN cord-274424-juj71nc5 100 11 by by IN cord-274424-juj71nc5 100 12 homologous homologous JJ cord-274424-juj71nc5 100 13 recombination recombination NN cord-274424-juj71nc5 100 14 into into IN cord-274424-juj71nc5 100 15 the the DT cord-274424-juj71nc5 100 16 TK TK NNP cord-274424-juj71nc5 100 17 locus locus NN cord-274424-juj71nc5 100 18 , , , cord-274424-juj71nc5 100 19 and and CC cord-274424-juj71nc5 100 20 Southern southern JJ cord-274424-juj71nc5 100 21 blot blot NN cord-274424-juj71nc5 100 22 analysis analysis NN cord-274424-juj71nc5 100 23 was be VBD cord-274424-juj71nc5 100 24 used use VBN cord-274424-juj71nc5 100 25 to to TO cord-274424-juj71nc5 100 26 confirm confirm VB cord-274424-juj71nc5 100 27 that that IN cord-274424-juj71nc5 100 28 the the DT cord-274424-juj71nc5 100 29 resulting result VBG cord-274424-juj71nc5 100 30 rVVs rvvs FW cord-274424-juj71nc5 100 31 vTS-1 vTS-1 NNP cord-274424-juj71nc5 100 32 and and CC cord-274424-juj71nc5 100 33 vTSA-1 vTSA-1 , cord-274424-juj71nc5 100 34 contained contain VBD cord-274424-juj71nc5 100 35 the the DT cord-274424-juj71nc5 100 36 4.6-and 4.6-and CD cord-274424-juj71nc5 100 37 3.45-kbp 3.45-kbp CD cord-274424-juj71nc5 100 38 TGEV TGEV NNP cord-274424-juj71nc5 100 39 BarnHI BarnHI NNP cord-274424-juj71nc5 100 40 gene gene NN cord-274424-juj71nc5 100 41 fragment fragment NN cord-274424-juj71nc5 100 42 , , , cord-274424-juj71nc5 100 43 respectively respectively RB cord-274424-juj71nc5 100 44 . . . cord-274424-juj71nc5 101 1 Two two CD cord-274424-juj71nc5 101 2 plaque plaque NN cord-274424-juj71nc5 101 3 - - HYPH cord-274424-juj71nc5 101 4 purified purify VBN cord-274424-juj71nc5 101 5 rVV rVV NNS cord-274424-juj71nc5 101 6 clones clone NNS cord-274424-juj71nc5 101 7 , , , cord-274424-juj71nc5 101 8 vTS-1 vTS-1 NNP cord-274424-juj71nc5 101 9 and and CC cord-274424-juj71nc5 101 10 vTS-2 vTS-2 NNP cord-274424-juj71nc5 101 11 , , , cord-274424-juj71nc5 101 12 were be VBD cord-274424-juj71nc5 101 13 used use VBN cord-274424-juj71nc5 101 14 to to TO cord-274424-juj71nc5 101 15 immunize immunize VB cord-274424-juj71nc5 102 1 Balb Balb NNP cord-274424-juj71nc5 102 2 / / SYM cord-274424-juj71nc5 102 3 C C NNP cord-274424-juj71nc5 102 4 mice mouse NNS cord-274424-juj71nc5 102 5 , , , cord-274424-juj71nc5 102 6 and and CC cord-274424-juj71nc5 102 7 the the DT cord-274424-juj71nc5 102 8 level level NN cord-274424-juj71nc5 102 9 of of IN cord-274424-juj71nc5 102 10 TGEV TGEV NNP cord-274424-juj71nc5 102 11 neutralizing neutralize VBG cord-274424-juj71nc5 102 12 antibodies antibody NNS cord-274424-juj71nc5 102 13 was be VBD cord-274424-juj71nc5 102 14 measured measure VBN cord-274424-juj71nc5 102 15 by by IN cord-274424-juj71nc5 102 16 a a DT cord-274424-juj71nc5 102 17 plaque plaque NN cord-274424-juj71nc5 102 18 reduction reduction NN cord-274424-juj71nc5 102 19 assay assay NN cord-274424-juj71nc5 102 20 ( ( -LRB- cord-274424-juj71nc5 102 21 Table table NN cord-274424-juj71nc5 102 22 1 1 CD cord-274424-juj71nc5 102 23 ) ) -RRB- cord-274424-juj71nc5 102 24 . . . cord-274424-juj71nc5 103 1 Both both DT cord-274424-juj71nc5 103 2 recombinant recombinant JJ cord-274424-juj71nc5 103 3 virus virus NN cord-274424-juj71nc5 103 4 clones clone NNS cord-274424-juj71nc5 103 5 induced induce VBN cord-274424-juj71nc5 103 6 TGEV TGEV NNP cord-274424-juj71nc5 103 7 neutralizing neutralize VBG cord-274424-juj71nc5 103 8 antibodies antibody NNS cord-274424-juj71nc5 103 9 that that WDT cord-274424-juj71nc5 103 10 were be VBD cord-274424-juj71nc5 103 11 boosted boost VBN cord-274424-juj71nc5 103 12 with with IN cord-274424-juj71nc5 103 13 a a DT cord-274424-juj71nc5 103 14 second second JJ cord-274424-juj71nc5 103 15 inoculation inoculation NN cord-274424-juj71nc5 103 16 . . . cord-274424-juj71nc5 104 1 Mice mouse NNS cord-274424-juj71nc5 104 2 previously previously RB cord-274424-juj71nc5 104 3 inoculated inoculate VBD cord-274424-juj71nc5 104 4 with with IN cord-274424-juj71nc5 104 5 the the DT cord-274424-juj71nc5 104 6 wild wild JJ cord-274424-juj71nc5 104 7 - - HYPH cord-274424-juj71nc5 104 8 type type NN cord-274424-juj71nc5 104 9 VV vv NN cord-274424-juj71nc5 104 10 and and CC cord-274424-juj71nc5 104 11 inoculated inoculate VBN cord-274424-juj71nc5 104 12 with with IN cord-274424-juj71nc5 104 13 vTS-1 vTS-1 NNP cord-274424-juj71nc5 104 14 produced produce VBD cord-274424-juj71nc5 104 15 a a DT cord-274424-juj71nc5 104 16 22-fold 22-fold CD cord-274424-juj71nc5 104 17 lower low JJR cord-274424-juj71nc5 104 18 level level NN cord-274424-juj71nc5 104 19 of of IN cord-274424-juj71nc5 104 20 TGEV TGEV NNP cord-274424-juj71nc5 104 21 neutralizing neutralize VBG cord-274424-juj71nc5 104 22 antibody antibody NN cord-274424-juj71nc5 104 23 compared compare VBN cord-274424-juj71nc5 104 24 to to IN cord-274424-juj71nc5 104 25 the the DT cord-274424-juj71nc5 104 26 convalescent convalescent JJ cord-274424-juj71nc5 104 27 serum serum NN cord-274424-juj71nc5 104 28 of of IN cord-274424-juj71nc5 104 29 vTS-1 vTS-1 NNP cord-274424-juj71nc5 104 30 . . . cord-274424-juj71nc5 105 1 A a DT cord-274424-juj71nc5 105 2 primary primary JJ cord-274424-juj71nc5 105 3 infection infection NN cord-274424-juj71nc5 105 4 with with IN cord-274424-juj71nc5 105 5 wild wild JJ cord-274424-juj71nc5 105 6 - - HYPH cord-274424-juj71nc5 105 7 type type NN cord-274424-juj71nc5 105 8 VV VV NNP cord-274424-juj71nc5 105 9 could could MD cord-274424-juj71nc5 105 10 have have VB cord-274424-juj71nc5 105 11 stimulated stimulate VBN cord-274424-juj71nc5 105 12 the the DT cord-274424-juj71nc5 105 13 mouse mouse NN cord-274424-juj71nc5 105 14 immune immune JJ cord-274424-juj71nc5 105 15 system system NN cord-274424-juj71nc5 105 16 FIG fig NN cord-274424-juj71nc5 105 17 . . . cord-274424-juj71nc5 106 1 1 1 LS cord-274424-juj71nc5 106 2 . . . cord-274424-juj71nc5 107 1 Construction construction NN cord-274424-juj71nc5 107 2 of of IN cord-274424-juj71nc5 107 3 the the DT cord-274424-juj71nc5 107 4 TGEV TGEV NNP cord-274424-juj71nc5 107 5 S S NNP cord-274424-juj71nc5 107 6 gene gene NN cord-274424-juj71nc5 107 7 from from IN cord-274424-juj71nc5 107 8 the the DT cord-274424-juj71nc5 107 9 FS772/70 FS772/70 NNP cord-274424-juj71nc5 107 10 cDNA cdna NN cord-274424-juj71nc5 107 11 . . . cord-274424-juj71nc5 108 1 The the DT cord-274424-juj71nc5 108 2 complete complete JJ cord-274424-juj71nc5 108 3 TGEV TGEV NNP cord-274424-juj71nc5 108 4 S S NNP cord-274424-juj71nc5 108 5 gene gene NN cord-274424-juj71nc5 108 6 was be VBD cord-274424-juj71nc5 108 7 generated generate VBN cord-274424-juj71nc5 108 8 by by IN cord-274424-juj71nc5 108 9 a a DT cord-274424-juj71nc5 108 10 four four CD cord-274424-juj71nc5 108 11 - - HYPH cord-274424-juj71nc5 108 12 way way NN cord-274424-juj71nc5 108 13 ligation ligation NN cord-274424-juj71nc5 108 14 into into IN cord-274424-juj71nc5 108 15 pBR322 pbr322 CD cord-274424-juj71nc5 108 16 . . . cord-274424-juj71nc5 109 1 The the DT cord-274424-juj71nc5 109 2 thin thin JJ cord-274424-juj71nc5 109 3 lines line NNS cord-274424-juj71nc5 109 4 represent represent VBP cord-274424-juj71nc5 109 5 TGEV TGEV NNP cord-274424-juj71nc5 109 6 cDNA cdna NN cord-274424-juj71nc5 109 7 and and CC cord-274424-juj71nc5 109 8 the the DT cord-274424-juj71nc5 109 9 thick thick JJ cord-274424-juj71nc5 109 10 lines line NNS cord-274424-juj71nc5 109 11 vector vector NN cord-274424-juj71nc5 109 12 DNA dna NN cord-274424-juj71nc5 109 13 sequence sequence NN cord-274424-juj71nc5 109 14 . . . cord-274424-juj71nc5 110 1 The the DT cord-274424-juj71nc5 110 2 top top JJ cord-274424-juj71nc5 110 3 line line NN cord-274424-juj71nc5 110 4 represents represent VBZ cord-274424-juj71nc5 110 5 the the DT cord-274424-juj71nc5 110 6 three three CD cord-274424-juj71nc5 110 7 cDNA cdna NN cord-274424-juj71nc5 110 8 clones clone NNS cord-274424-juj71nc5 110 9 used use VBN cord-274424-juj71nc5 110 10 to to TO cord-274424-juj71nc5 110 11 generate generate VB cord-274424-juj71nc5 110 12 specific specific JJ cord-274424-juj71nc5 110 13 cDNA cdna NN cord-274424-juj71nc5 110 14 fragments fragment NNS cord-274424-juj71nc5 110 15 . . . cord-274424-juj71nc5 111 1 If if IN cord-274424-juj71nc5 111 2 more more JJR cord-274424-juj71nc5 111 3 than than IN cord-274424-juj71nc5 111 4 one one CD cord-274424-juj71nc5 111 5 enzyme enzyme NN cord-274424-juj71nc5 111 6 was be VBD cord-274424-juj71nc5 111 7 used use VBN cord-274424-juj71nc5 111 8 they -PRON- PRP cord-274424-juj71nc5 111 9 are be VBP cord-274424-juj71nc5 111 10 listed list VBN cord-274424-juj71nc5 111 11 in in IN cord-274424-juj71nc5 111 12 a a DT cord-274424-juj71nc5 111 13 descending descend VBG cord-274424-juj71nc5 111 14 order order NN cord-274424-juj71nc5 111 15 next next RB cord-274424-juj71nc5 111 16 to to IN cord-274424-juj71nc5 111 17 the the DT cord-274424-juj71nc5 111 18 appropriate appropriate JJ cord-274424-juj71nc5 111 19 arrow arrow NN cord-274424-juj71nc5 111 20 . . . cord-274424-juj71nc5 112 1 The the DT cord-274424-juj71nc5 112 2 0.4-bp 0.4-bp CD cord-274424-juj71nc5 112 3 Hpal Hpal NNP cord-274424-juj71nc5 112 4 - - HYPH cord-274424-juj71nc5 112 5 Pstl Pstl NNP cord-274424-juj71nc5 112 6 fragment fragment NN cord-274424-juj71nc5 112 7 from from IN cord-274424-juj71nc5 112 8 pTG47 ptg47 NN cord-274424-juj71nc5 112 9 had have VBD cord-274424-juj71nc5 112 10 BarnHI BarnHI NNP cord-274424-juj71nc5 112 11 linkers linker NNS cord-274424-juj71nc5 112 12 added add VBD cord-274424-juj71nc5 112 13 and and CC cord-274424-juj71nc5 112 14 was be VBD cord-274424-juj71nc5 112 15 then then RB cord-274424-juj71nc5 112 16 digested digest VBN cord-274424-juj71nc5 112 17 with with IN cord-274424-juj71nc5 112 18 BarnHI BarnHI NNP cord-274424-juj71nc5 112 19 and and CC cord-274424-juj71nc5 112 20 Sty1 Sty1 NNP cord-274424-juj71nc5 112 21 to to TO cord-274424-juj71nc5 112 22 generate generate VB cord-274424-juj71nc5 112 23 a a DT cord-274424-juj71nc5 112 24 0.2.kb 0.2.kb CD cord-274424-juj71nc5 112 25 fragment fragment NN cord-274424-juj71nc5 112 26 with with IN cord-274424-juj71nc5 112 27 a a DT cord-274424-juj71nc5 112 28 BarnHI BarnHI NNP cord-274424-juj71nc5 112 29 site site NN cord-274424-juj71nc5 112 30 upstream upstream RB cord-274424-juj71nc5 112 31 of of IN cord-274424-juj71nc5 112 32 the the DT cord-274424-juj71nc5 112 33 S S NNP cord-274424-juj71nc5 112 34 gene gene NN cord-274424-juj71nc5 112 35 initiation initiation NN cord-274424-juj71nc5 112 36 codon codon NN cord-274424-juj71nc5 112 37 . . . cord-274424-juj71nc5 113 1 The the DT cord-274424-juj71nc5 113 2 1.29.kb 1.29.kb CD cord-274424-juj71nc5 113 3 HindIll HindIll NNP cord-274424-juj71nc5 113 4 - - HYPH cord-274424-juj71nc5 113 5 HindIll HindIll NNP cord-274424-juj71nc5 113 6 fragment fragment NN cord-274424-juj71nc5 113 7 from from IN cord-274424-juj71nc5 113 8 pTG30 pTG30 NNS cord-274424-juj71nc5 113 9 was be VBD cord-274424-juj71nc5 113 10 initially initially RB cord-274424-juj71nc5 113 11 cloned clone VBN cord-274424-juj71nc5 113 12 into into IN cord-274424-juj71nc5 113 13 pUC9 pUC9 NNP cord-274424-juj71nc5 113 14 and and CC cord-274424-juj71nc5 113 15 removed remove VBD cord-274424-juj71nc5 113 16 as as IN cord-274424-juj71nc5 113 17 a a DT cord-274424-juj71nc5 113 18 Xbal Xbal NNP cord-274424-juj71nc5 113 19 - - HYPH cord-274424-juj71nc5 113 20 BarnHI BarnHI NNP cord-274424-juj71nc5 113 21 fragment fragment NN cord-274424-juj71nc5 113 22 , , , cord-274424-juj71nc5 113 23 with with IN cord-274424-juj71nc5 113 24 the the DT cord-274424-juj71nc5 113 25 BarnHI BarnHI NNP cord-274424-juj71nc5 113 26 site site NN cord-274424-juj71nc5 113 27 derived derive VBN cord-274424-juj71nc5 113 28 from from IN cord-274424-juj71nc5 113 29 the the DT cord-274424-juj71nc5 113 30 pUC9 pUC9 NNP cord-274424-juj71nc5 113 31 polylinker polylinker NN cord-274424-juj71nc5 113 32 sequence sequence NN cord-274424-juj71nc5 113 33 , , , cord-274424-juj71nc5 113 34 to to TO cord-274424-juj71nc5 113 35 provide provide VB cord-274424-juj71nc5 113 36 a a DT cord-274424-juj71nc5 113 37 BarnHI BarnHI NNP cord-274424-juj71nc5 113 38 site site NN cord-274424-juj71nc5 113 39 within within IN cord-274424-juj71nc5 113 40 the the DT cord-274424-juj71nc5 113 41 TGEV TGEV NNP cord-274424-juj71nc5 113 42 ORF-3a ORF-3a NNP cord-274424-juj71nc5 113 43 gene gene NN cord-274424-juj71nc5 113 44 3'to 3'to CD cord-274424-juj71nc5 113 45 the the DT cord-274424-juj71nc5 113 46 end end NN cord-274424-juj71nc5 113 47 of of IN cord-274424-juj71nc5 113 48 the the DT cord-274424-juj71nc5 113 49 S S NNP cord-274424-juj71nc5 113 50 gene gene NN cord-274424-juj71nc5 113 51 . . . cord-274424-juj71nc5 114 1 The the DT cord-274424-juj71nc5 114 2 relevant relevant JJ cord-274424-juj71nc5 114 3 fragments fragment NNS cord-274424-juj71nc5 114 4 were be VBD cord-274424-juj71nc5 114 5 then then RB cord-274424-juj71nc5 114 6 ligated ligate VBN cord-274424-juj71nc5 114 7 together together RB cord-274424-juj71nc5 114 8 with with IN cord-274424-juj71nc5 114 9 pBR322 pbr322 CD cord-274424-juj71nc5 114 10 such such JJ cord-274424-juj71nc5 114 11 that that IN cord-274424-juj71nc5 114 12 only only RB cord-274424-juj71nc5 114 13 the the DT cord-274424-juj71nc5 114 14 correct correct JJ cord-274424-juj71nc5 114 15 alignment alignment NN cord-274424-juj71nc5 114 16 of of IN cord-274424-juj71nc5 114 17 the the DT cord-274424-juj71nc5 114 18 cohesive cohesive JJ cord-274424-juj71nc5 114 19 ends end NNS cord-274424-juj71nc5 114 20 would would MD cord-274424-juj71nc5 114 21 give give VB cord-274424-juj71nc5 114 22 a a DT cord-274424-juj71nc5 114 23 fragment fragment NN cord-274424-juj71nc5 114 24 of of IN cord-274424-juj71nc5 114 25 4.6 4.6 CD cord-274424-juj71nc5 114 26 kb kb NNP cord-274424-juj71nc5 114 27 in in IN cord-274424-juj71nc5 114 28 pBR322 pBR322 NNP cord-274424-juj71nc5 114 29 corresponding correspond VBG cord-274424-juj71nc5 114 30 to to IN cord-274424-juj71nc5 114 31 the the DT cord-274424-juj71nc5 114 32 TGEV TGEV NNP cord-274424-juj71nc5 114 33 S S NNP cord-274424-juj71nc5 114 34 gene gene NN cord-274424-juj71nc5 114 35 BamHl bamhl NN cord-274424-juj71nc5 114 36 cassette cassette NN cord-274424-juj71nc5 114 37 . . . cord-274424-juj71nc5 115 1 into into IN cord-274424-juj71nc5 115 2 producing produce VBG cord-274424-juj71nc5 115 3 a a DT cord-274424-juj71nc5 115 4 rapid rapid JJ cord-274424-juj71nc5 115 5 clearance clearance NN cord-274424-juj71nc5 115 6 of of IN cord-274424-juj71nc5 115 7 a a DT cord-274424-juj71nc5 115 8 subsequent subsequent JJ cord-274424-juj71nc5 115 9 rW rw DT cord-274424-juj71nc5 115 10 infection infection NN cord-274424-juj71nc5 115 11 and and CC cord-274424-juj71nc5 115 12 prevented prevent VBD cord-274424-juj71nc5 115 13 the the DT cord-274424-juj71nc5 115 14 expression expression NN cord-274424-juj71nc5 115 15 of of IN cord-274424-juj71nc5 115 16 large large JJ cord-274424-juj71nc5 115 17 amounts amount NNS cord-274424-juj71nc5 115 18 of of IN cord-274424-juj71nc5 115 19 S s NN cord-274424-juj71nc5 115 20 protein protein NN cord-274424-juj71nc5 115 21 . . . cord-274424-juj71nc5 116 1 Mice mouse NNS cord-274424-juj71nc5 116 2 given give VBD cord-274424-juj71nc5 116 3 a a DT cord-274424-juj71nc5 116 4 single single JJ cord-274424-juj71nc5 116 5 inoculation inoculation NN cord-274424-juj71nc5 116 6 with with IN cord-274424-juj71nc5 116 7 vTS-1 vTS-1 NNP cord-274424-juj71nc5 116 8 4 4 CD cord-274424-juj71nc5 116 9 weeks week NNS cord-274424-juj71nc5 116 10 after after IN cord-274424-juj71nc5 116 11 the the DT cord-274424-juj71nc5 116 12 other other JJ cord-274424-juj71nc5 116 13 mouse mouse NN cord-274424-juj71nc5 116 14 group group NN cord-274424-juj71nc5 116 15 had have VBD cord-274424-juj71nc5 116 16 a a DT cord-274424-juj71nc5 116 17 fourfold fourfold JJ cord-274424-juj71nc5 116 18 reduction reduction NN cord-274424-juj71nc5 116 19 in in IN cord-274424-juj71nc5 116 20 the the DT cord-274424-juj71nc5 116 21 level level NN cord-274424-juj71nc5 116 22 of of IN cord-274424-juj71nc5 116 23 TGEV TGEV NNP cord-274424-juj71nc5 116 24 neutralizing neutralize VBG cord-274424-juj71nc5 116 25 antibody antibody NN cord-274424-juj71nc5 116 26 . . . cord-274424-juj71nc5 117 1 This this DT cord-274424-juj71nc5 117 2 suggested suggest VBD cord-274424-juj71nc5 117 3 that that IN cord-274424-juj71nc5 117 4 age age NN cord-274424-juj71nc5 117 5 may may MD cord-274424-juj71nc5 117 6 have have VB cord-274424-juj71nc5 117 7 a a DT cord-274424-juj71nc5 117 8 significant significant JJ cord-274424-juj71nc5 117 9 effect effect NN cord-274424-juj71nc5 117 10 on on IN cord-274424-juj71nc5 117 11 the the DT cord-274424-juj71nc5 117 12 induction induction NN cord-274424-juj71nc5 117 13 of of IN cord-274424-juj71nc5 117 14 TGEV TGEV NNP cord-274424-juj71nc5 117 15 neutralizing neutralize VBG cord-274424-juj71nc5 117 16 antibodies antibody NNS cord-274424-juj71nc5 117 17 , , , cord-274424-juj71nc5 117 18 but but CC cord-274424-juj71nc5 117 19 further further JJ cord-274424-juj71nc5 117 20 animal animal NN cord-274424-juj71nc5 117 21 studies study NNS cord-274424-juj71nc5 117 22 are be VBP cord-274424-juj71nc5 117 23 needed need VBN cord-274424-juj71nc5 117 24 to to TO cord-274424-juj71nc5 117 25 make make VB cord-274424-juj71nc5 117 26 any any DT cord-274424-juj71nc5 117 27 firm firm JJ cord-274424-juj71nc5 117 28 conclusions conclusion NNS cord-274424-juj71nc5 117 29 . . . cord-274424-juj71nc5 118 1 Expression expression NN cord-274424-juj71nc5 118 2 of of IN cord-274424-juj71nc5 118 3 the the DT cord-274424-juj71nc5 118 4 recombinant recombinant JJ cord-274424-juj71nc5 118 5 spike spike NN cord-274424-juj71nc5 118 6 antigens antigen NNS cord-274424-juj71nc5 118 7 TGEV TGEV NNP cord-274424-juj71nc5 118 8 gene gene NN cord-274424-juj71nc5 118 9 products product NNS cord-274424-juj71nc5 118 10 synthesized synthesize VBN cord-274424-juj71nc5 118 11 by by IN cord-274424-juj71nc5 118 12 vTS-1 vTS-1 NNP cord-274424-juj71nc5 118 13 and and CC cord-274424-juj71nc5 118 14 vTSA-1 vTSA-1 , cord-274424-juj71nc5 118 15 were be VBD cord-274424-juj71nc5 118 16 analyzed analyze VBN cord-274424-juj71nc5 118 17 by by IN cord-274424-juj71nc5 118 18 pulse pulse NN cord-274424-juj71nc5 118 19 labeling labeling NN cord-274424-juj71nc5 118 20 rVV rvv RB cord-274424-juj71nc5 118 21 - - HYPH cord-274424-juj71nc5 118 22 infected infect VBN cord-274424-juj71nc5 118 23 HTK HTK NNP cord-274424-juj71nc5 118 24 - - HYPH cord-274424-juj71nc5 118 25 cells cell NNS cord-274424-juj71nc5 118 26 with with IN cord-274424-juj71nc5 118 27 L-[35S]methionine l-[35s]methionine NN cord-274424-juj71nc5 118 28 in in IN cord-274424-juj71nc5 118 29 the the DT cord-274424-juj71nc5 118 30 presence presence NN cord-274424-juj71nc5 118 31 or or CC cord-274424-juj71nc5 118 32 absence absence NN cord-274424-juj71nc5 118 33 of of IN cord-274424-juj71nc5 118 34 10 10 CD cord-274424-juj71nc5 118 35 pg pg NN cord-274424-juj71nc5 118 36 ml- ml- NN cord-274424-juj71nc5 118 37 ' ' `` cord-274424-juj71nc5 118 38 tunicamycin tunicamycin NNS cord-274424-juj71nc5 118 39 for for IN cord-274424-juj71nc5 118 40 6 6 CD cord-274424-juj71nc5 118 41 hr hr NN cord-274424-juj71nc5 118 42 . . . cord-274424-juj71nc5 119 1 TGEV TGEV NNP cord-274424-juj71nc5 119 2 S S NNP cord-274424-juj71nc5 119 3 protein protein NN cord-274424-juj71nc5 119 4 was be VBD cord-274424-juj71nc5 119 5 immunoprecipitated immunoprecipitate VBN cord-274424-juj71nc5 119 6 from from IN cord-274424-juj71nc5 119 7 cell cell NN cord-274424-juj71nc5 119 8 lysates lysate NNS cord-274424-juj71nc5 119 9 or or CC cord-274424-juj71nc5 119 10 tissue tissue NN cord-274424-juj71nc5 119 11 culture culture NN cord-274424-juj71nc5 119 12 fluids fluid NNS cord-274424-juj71nc5 119 13 using use VBG cord-274424-juj71nc5 119 14 porcine porcine JJ cord-274424-juj71nc5 119 15 TGEV TGEV NNP cord-274424-juj71nc5 119 16 hyperimmune hyperimmune NNP cord-274424-juj71nc5 119 17 antiserum antiserum NN cord-274424-juj71nc5 119 18 . . . cord-274424-juj71nc5 120 1 This this DT cord-274424-juj71nc5 120 2 resulted result VBD cord-274424-juj71nc5 120 3 in in IN cord-274424-juj71nc5 120 4 S S NNP cord-274424-juj71nc5 120 5 polypeptides polypeptide NNS cord-274424-juj71nc5 120 6 of of IN cord-274424-juj71nc5 120 7 AJ AJ NNP cord-274424-juj71nc5 120 8 190,000 190,000 CD cord-274424-juj71nc5 120 9 ( ( -LRB- cord-274424-juj71nc5 120 10 gpl90 gpl90 NNP cord-274424-juj71nc5 120 11 ) ) -RRB- cord-274424-juj71nc5 120 12 and and CC cord-274424-juj71nc5 120 13 AJ AJ NNP cord-274424-juj71nc5 120 14 210,000 210,000 CD cord-274424-juj71nc5 120 15 ( ( -LRB- cord-274424-juj71nc5 120 16 gp210 gp210 NNP cord-274424-juj71nc5 120 17 ) ) -RRB- cord-274424-juj71nc5 120 18 being be VBG cord-274424-juj71nc5 120 19 immunoprecipitated immunoprecipitate VBN cord-274424-juj71nc5 120 20 from from IN cord-274424-juj71nc5 120 21 vTS-1 vTS-1 NNP cord-274424-juj71nc5 120 22 -infected -infected JJ cord-274424-juj71nc5 120 23 cell cell NN cord-274424-juj71nc5 120 24 lysates lysate NNS cord-274424-juj71nc5 120 25 in in IN cord-274424-juj71nc5 120 26 the the DT cord-274424-juj71nc5 120 27 absence absence NN cord-274424-juj71nc5 120 28 of of IN cord-274424-juj71nc5 120 29 tunicamycin tunicamycin NNS cord-274424-juj71nc5 120 30 ( ( -LRB- cord-274424-juj71nc5 120 31 Fig Fig NNP cord-274424-juj71nc5 120 32 . . . cord-274424-juj71nc5 120 33 3 3 CD cord-274424-juj71nc5 120 34 , , , cord-274424-juj71nc5 120 35 lane lane NN cord-274424-juj71nc5 120 36 2 2 CD cord-274424-juj71nc5 120 37 ) ) -RRB- cord-274424-juj71nc5 120 38 while while IN cord-274424-juj71nc5 120 39 in in IN cord-274424-juj71nc5 120 40 the the DT cord-274424-juj71nc5 120 41 presence presence NN cord-274424-juj71nc5 120 42 of of IN cord-274424-juj71nc5 120 43 tunicamycin tunicamycin NNP cord-274424-juj71nc5 120 44 , , , cord-274424-juj71nc5 120 45 a a DT cord-274424-juj71nc5 120 46 AA AA NNP cord-274424-juj71nc5 120 47 , , , cord-274424-juj71nc5 120 48 160,000 160,000 CD cord-274424-juj71nc5 120 49 ( ( -LRB- cord-274424-juj71nc5 120 50 ~160 ~160 NN cord-274424-juj71nc5 120 51 ) ) -RRB- cord-274424-juj71nc5 120 52 precursor precursor NN cord-274424-juj71nc5 120 53 S S NNP cord-274424-juj71nc5 120 54 polypeptide polypeptide NN cord-274424-juj71nc5 120 55 was be VBD cord-274424-juj71nc5 120 56 synthesized synthesize VBN cord-274424-juj71nc5 120 57 ( ( -LRB- cord-274424-juj71nc5 120 58 Fig Fig NNP cord-274424-juj71nc5 120 59 . . . cord-274424-juj71nc5 120 60 3 3 CD cord-274424-juj71nc5 120 61 , , , cord-274424-juj71nc5 120 62 lane lane NN cord-274424-juj71nc5 120 63 3 3 CD cord-274424-juj71nc5 120 64 ) ) -RRB- cord-274424-juj71nc5 120 65 . . . cord-274424-juj71nc5 121 1 Cells cell NNS cord-274424-juj71nc5 121 2 infected infect VBN cord-274424-juj71nc5 121 3 with with IN cord-274424-juj71nc5 121 4 vTSA-1 vtsa-1 ADD cord-274424-juj71nc5 121 5 expressed express VBD cord-274424-juj71nc5 121 6 a a DT cord-274424-juj71nc5 121 7 M M NNP cord-274424-juj71nc5 121 8 , , , cord-274424-juj71nc5 121 9 170,000 170,000 CD cord-274424-juj71nc5 121 10 glycosylated glycosylated JJ cord-274424-juj71nc5 121 11 polypeptide polypeptide NN cord-274424-juj71nc5 121 12 ( ( -LRB- cord-274424-juj71nc5 121 13 gpl70 gpl70 CD cord-274424-juj71nc5 121 14 ) ) -RRB- cord-274424-juj71nc5 121 15 only only RB cord-274424-juj71nc5 121 16 ( ( -LRB- cord-274424-juj71nc5 121 17 Fig Fig NNP cord-274424-juj71nc5 121 18 . . . cord-274424-juj71nc5 121 19 3 3 CD cord-274424-juj71nc5 121 20 , , , cord-274424-juj71nc5 121 21 lane lane NN cord-274424-juj71nc5 121 22 4 4 CD cord-274424-juj71nc5 121 23 ) ) -RRB- cord-274424-juj71nc5 121 24 that that WDT cord-274424-juj71nc5 121 25 was be VBD cord-274424-juj71nc5 121 26 expressed express VBN cord-274424-juj71nc5 121 27 as as IN cord-274424-juj71nc5 121 28 a a DT cord-274424-juj71nc5 121 29 AA AA NNP cord-274424-juj71nc5 121 30 , , , cord-274424-juj71nc5 121 31 130,000 130,000 CD cord-274424-juj71nc5 121 32 ( ( -LRB- cord-274424-juj71nc5 121 33 ~130 ~130 NFP cord-274424-juj71nc5 121 34 ) ) -RRB- cord-274424-juj71nc5 121 35 precursor precursor NN cord-274424-juj71nc5 121 36 protein protein NN cord-274424-juj71nc5 121 37 ( ( -LRB- cord-274424-juj71nc5 121 38 Fig Fig NNP cord-274424-juj71nc5 121 39 . . . cord-274424-juj71nc5 121 40 3 3 CD cord-274424-juj71nc5 121 41 , , , cord-274424-juj71nc5 121 42 lane lane NN cord-274424-juj71nc5 121 43 5 5 CD cord-274424-juj71nc5 121 44 ) ) -RRB- cord-274424-juj71nc5 121 45 in in IN cord-274424-juj71nc5 121 46 the the DT cord-274424-juj71nc5 121 47 presence presence NN cord-274424-juj71nc5 121 48 of of IN cord-274424-juj71nc5 121 49 tunicamycin tunicamycin NNS cord-274424-juj71nc5 121 50 . . . cord-274424-juj71nc5 122 1 It -PRON- PRP cord-274424-juj71nc5 122 2 was be VBD cord-274424-juj71nc5 122 3 noted note VBN cord-274424-juj71nc5 122 4 that that IN cord-274424-juj71nc5 122 5 the the DT cord-274424-juj71nc5 122 6 levels level NNS cord-274424-juj71nc5 122 7 of of IN cord-274424-juj71nc5 122 8 S S NNP cord-274424-juj71nc5 122 9 protein protein NN cord-274424-juj71nc5 122 10 expressed express VBD cord-274424-juj71nc5 122 11 were be VBD cord-274424-juj71nc5 122 12 appreciably appreciably RB cord-274424-juj71nc5 122 13 less less JJR cord-274424-juj71nc5 122 14 in in IN cord-274424-juj71nc5 122 15 the the DT cord-274424-juj71nc5 122 16 presence presence NN cord-274424-juj71nc5 122 17 of of IN cord-274424-juj71nc5 122 18 tunicamycin tunicamycin NNS cord-274424-juj71nc5 122 19 ( ( -LRB- cord-274424-juj71nc5 122 20 Fig Fig NNP cord-274424-juj71nc5 122 21 . . . cord-274424-juj71nc5 122 22 3 3 CD cord-274424-juj71nc5 122 23 ) ) -RRB- cord-274424-juj71nc5 122 24 . . . cord-274424-juj71nc5 123 1 Comparison comparison NN cord-274424-juj71nc5 123 2 of of IN cord-274424-juj71nc5 123 3 the the DT cord-274424-juj71nc5 123 4 nonspecifically nonspecifically RB cord-274424-juj71nc5 123 5 precipitated precipitate VBN cord-274424-juj71nc5 123 6 proteins protein NNS cord-274424-juj71nc5 123 7 in in IN cord-274424-juj71nc5 123 8 the the DT cord-274424-juj71nc5 123 9 presence presence NN cord-274424-juj71nc5 123 10 or or CC cord-274424-juj71nc5 123 11 absence absence NN cord-274424-juj71nc5 123 12 of of IN cord-274424-juj71nc5 123 13 tunicamycin tunicamycin NNS cord-274424-juj71nc5 123 14 ( ( -LRB- cord-274424-juj71nc5 123 15 Fig Fig NNP cord-274424-juj71nc5 123 16 . . . cord-274424-juj71nc5 123 17 3 3 LS cord-274424-juj71nc5 123 18 ) ) -RRB- cord-274424-juj71nc5 123 19 indicated indicate VBD cord-274424-juj71nc5 123 20 that that IN cord-274424-juj71nc5 123 21 the the DT cord-274424-juj71nc5 123 22 reduction reduction NN cord-274424-juj71nc5 123 23 in in IN cord-274424-juj71nc5 123 24 S S NNP cord-274424-juj71nc5 123 25 protein protein NN cord-274424-juj71nc5 123 26 synthesis synthesis NN cord-274424-juj71nc5 123 27 was be VBD cord-274424-juj71nc5 123 28 not not RB cord-274424-juj71nc5 123 29 due due JJ cord-274424-juj71nc5 123 30 to to IN cord-274424-juj71nc5 123 31 inhibition inhibition NN cord-274424-juj71nc5 123 32 of of IN cord-274424-juj71nc5 123 33 viral viral JJ cord-274424-juj71nc5 123 34 replication replication NN cord-274424-juj71nc5 123 35 in in IN cord-274424-juj71nc5 123 36 the the DT cord-274424-juj71nc5 123 37 presence presence NN cord-274424-juj71nc5 123 38 of of IN cord-274424-juj71nc5 123 39 tunicamycin tunicamycin NNS cord-274424-juj71nc5 123 40 . . . cord-274424-juj71nc5 124 1 These these DT cord-274424-juj71nc5 124 2 observations observation NNS cord-274424-juj71nc5 124 3 suggested suggest VBD cord-274424-juj71nc5 124 4 therefore therefore RB cord-274424-juj71nc5 124 5 that that IN cord-274424-juj71nc5 124 6 either either DT cord-274424-juj71nc5 124 7 glycosylation glycosylation NN cord-274424-juj71nc5 124 8 was be VBD cord-274424-juj71nc5 124 9 required require VBN cord-274424-juj71nc5 124 10 for for IN cord-274424-juj71nc5 124 11 efficient efficient JJ cord-274424-juj71nc5 124 12 synthesis synthesis NN cord-274424-juj71nc5 124 13 of of IN cord-274424-juj71nc5 124 14 the the DT cord-274424-juj71nc5 124 15 S S NNP cord-274424-juj71nc5 124 16 protein protein NN cord-274424-juj71nc5 124 17 or or CC cord-274424-juj71nc5 124 18 the the DT cord-274424-juj71nc5 124 19 majority majority NN cord-274424-juj71nc5 124 20 of of IN cord-274424-juj71nc5 124 21 the the DT cord-274424-juj71nc5 124 22 anti anti JJ cord-274424-juj71nc5 124 23 - - JJ cord-274424-juj71nc5 124 24 S s JJ cord-274424-juj71nc5 124 25 antibodies antibody NNS cord-274424-juj71nc5 124 26 were be VBD cord-274424-juj71nc5 124 27 directed direct VBN cord-274424-juj71nc5 124 28 against against IN cord-274424-juj71nc5 124 29 the the DT cord-274424-juj71nc5 124 30 glycosylated glycosylated JJ cord-274424-juj71nc5 124 31 form form NN cord-274424-juj71nc5 124 32 of of IN cord-274424-juj71nc5 124 33 the the DT cord-274424-juj71nc5 124 34 protein protein NN cord-274424-juj71nc5 124 35 resulting result VBG cord-274424-juj71nc5 124 36 in in IN cord-274424-juj71nc5 124 37 less less RBR cord-274424-juj71nc5 124 38 efficient efficient JJ cord-274424-juj71nc5 124 39 immunoprecipitation immunoprecipitation NN cord-274424-juj71nc5 124 40 of of IN cord-274424-juj71nc5 124 41 the the DT cord-274424-juj71nc5 124 42 nonglycosylated nonglycosylate VBN cord-274424-juj71nc5 124 43 form form NN cord-274424-juj71nc5 124 44 . . . cord-274424-juj71nc5 125 1 Although although IN cord-274424-juj71nc5 125 2 gp210 gp210 NNP cord-274424-juj71nc5 125 3 was be VBD cord-274424-juj71nc5 125 4 immunoprecipitated immunoprecipitate VBN cord-274424-juj71nc5 125 5 from from IN cord-274424-juj71nc5 125 6 the the DT cord-274424-juj71nc5 125 7 vTS-1 vTS-1 NNP cord-274424-juj71nc5 125 8 -infected -infected JJ cord-274424-juj71nc5 125 9 cell cell NN cord-274424-juj71nc5 125 10 culture culture NN cord-274424-juj71nc5 125 11 medium medium NN cord-274424-juj71nc5 125 12 in in IN cord-274424-juj71nc5 125 13 the the DT cord-274424-juj71nc5 125 14 absence absence NN cord-274424-juj71nc5 125 15 of of IN cord-274424-juj71nc5 125 16 tunicamycin tunicamycin NNS cord-274424-juj71nc5 125 17 ( ( -LRB- cord-274424-juj71nc5 125 18 Fig Fig NNP cord-274424-juj71nc5 125 19 . . NNP cord-274424-juj71nc5 125 20 4 4 CD cord-274424-juj71nc5 125 21 , , , cord-274424-juj71nc5 125 22 lane lane NN cord-274424-juj71nc5 125 23 3 3 CD cord-274424-juj71nc5 125 24 ) ) -RRB- cord-274424-juj71nc5 126 1 no no DT cord-274424-juj71nc5 126 2 detectable detectable JJ cord-274424-juj71nc5 126 3 extracellular extracellular JJ cord-274424-juj71nc5 126 4 SA SA NNP cord-274424-juj71nc5 126 5 protein protein NN cord-274424-juj71nc5 126 6 ( ( -LRB- cord-274424-juj71nc5 126 7 gpl70 gpl70 NNP cord-274424-juj71nc5 126 8 ) ) -RRB- cord-274424-juj71nc5 126 9 was be VBD cord-274424-juj71nc5 126 10 recovered recover VBN cord-274424-juj71nc5 126 11 from from IN cord-274424-juj71nc5 126 12 medium medium NN cord-274424-juj71nc5 126 13 of of IN cord-274424-juj71nc5 126 14 vTSA vtsa NN cord-274424-juj71nc5 126 15 - - HYPH cord-274424-juj71nc5 126 16 l l NN cord-274424-juj71nc5 126 17 - - HYPH cord-274424-juj71nc5 126 18 infected infect VBN cord-274424-juj71nc5 126 19 cells cell NNS cord-274424-juj71nc5 127 1 ( ( -LRB- cord-274424-juj71nc5 127 2 Fig Fig NNP cord-274424-juj71nc5 127 3 . . NNP cord-274424-juj71nc5 127 4 4 4 CD cord-274424-juj71nc5 127 5 , , , cord-274424-juj71nc5 127 6 lane lane NN cord-274424-juj71nc5 127 7 6 6 CD cord-274424-juj71nc5 127 8 ) ) -RRB- cord-274424-juj71nc5 127 9 suggesting suggest VBG cord-274424-juj71nc5 127 10 that that IN cord-274424-juj71nc5 127 11 the the DT cord-274424-juj71nc5 127 12 membrane membrane NN cord-274424-juj71nc5 127 13 anchor anchor NN cord-274424-juj71nc5 127 14 is be VBZ cord-274424-juj71nc5 127 15 required require VBN cord-274424-juj71nc5 127 16 for for IN cord-274424-juj71nc5 127 17 export export NN cord-274424-juj71nc5 127 18 out out IN cord-274424-juj71nc5 127 19 of of IN cord-274424-juj71nc5 127 20 cells cell NNS cord-274424-juj71nc5 127 21 . . . cord-274424-juj71nc5 128 1 The the DT cord-274424-juj71nc5 128 2 lack lack NN cord-274424-juj71nc5 128 3 of of IN cord-274424-juj71nc5 128 4 detectable detectable JJ cord-274424-juj71nc5 128 5 ~160 ~160 NN cord-274424-juj71nc5 128 6 or or CC cord-274424-juj71nc5 128 7 ~130 ~130 . cord-274424-juj71nc5 128 8 in in IN cord-274424-juj71nc5 128 9 the the DT cord-274424-juj71nc5 128 10 culture culture NN cord-274424-juj71nc5 128 11 medium medium NN cord-274424-juj71nc5 128 12 of of IN cord-274424-juj71nc5 128 13 vTS vTS NNP cord-274424-juj71nc5 128 14 - - HYPH cord-274424-juj71nc5 128 15 l l NN cord-274424-juj71nc5 128 16 - - HYPH cord-274424-juj71nc5 128 17 and and CC cord-274424-juj71nc5 128 18 vTSA vtsa NN cord-274424-juj71nc5 128 19 - - HYPH cord-274424-juj71nc5 128 20 l l NN cord-274424-juj71nc5 128 21 - - HYPH cord-274424-juj71nc5 128 22 infected infect VBN cord-274424-juj71nc5 128 23 cells cell NNS cord-274424-juj71nc5 128 24 , , , cord-274424-juj71nc5 128 25 respectively respectively RB cord-274424-juj71nc5 128 26 ( ( -LRB- cord-274424-juj71nc5 128 27 Fig Fig NNP cord-274424-juj71nc5 128 28 . . . cord-274424-juj71nc5 128 29 4 4 CD cord-274424-juj71nc5 128 30 lanes lane NNS cord-274424-juj71nc5 128 31 4 4 CD cord-274424-juj71nc5 128 32 and and CC cord-274424-juj71nc5 128 33 _SP cord-274424-juj71nc5 128 34 7 7 CD cord-274424-juj71nc5 128 35 ) ) -RRB- cord-274424-juj71nc5 128 36 , , , cord-274424-juj71nc5 128 37 could could MD cord-274424-juj71nc5 128 38 result result VB cord-274424-juj71nc5 128 39 from from IN cord-274424-juj71nc5 128 40 the the DT cord-274424-juj71nc5 128 41 levels level NNS cord-274424-juj71nc5 128 42 being be VBG cord-274424-juj71nc5 128 43 too too RB cord-274424-juj71nc5 128 44 low low JJ cord-274424-juj71nc5 128 45 to to TO cord-274424-juj71nc5 128 46 be be VB cord-274424-juj71nc5 128 47 detected detect VBN cord-274424-juj71nc5 128 48 by by IN cord-274424-juj71nc5 128 49 the the DT cord-274424-juj71nc5 128 50 antibodies antibody NNS cord-274424-juj71nc5 128 51 due due JJ cord-274424-juj71nc5 128 52 to to IN cord-274424-juj71nc5 128 53 reduction reduction NN cord-274424-juj71nc5 128 54 in in IN cord-274424-juj71nc5 128 55 synthesis synthesis NN cord-274424-juj71nc5 128 56 or or CC cord-274424-juj71nc5 128 57 the the DT cord-274424-juj71nc5 128 58 lack lack NN cord-274424-juj71nc5 128 59 of of IN cord-274424-juj71nc5 128 60 glycosylation glycosylation NN cord-274424-juj71nc5 128 61 of of IN cord-274424-juj71nc5 128 62 the the DT cord-274424-juj71nc5 128 63 S S NNP cord-274424-juj71nc5 128 64 protein protein NN cord-274424-juj71nc5 128 65 in in IN cord-274424-juj71nc5 128 66 the the DT cord-274424-juj71nc5 128 67 presence presence NN cord-274424-juj71nc5 128 68 of of IN cord-274424-juj71nc5 128 69 tunicamycin tunicamycin NNS cord-274424-juj71nc5 128 70 . . . cord-274424-juj71nc5 129 1 The the DT cord-274424-juj71nc5 129 2 rate rate NN cord-274424-juj71nc5 129 3 of of IN cord-274424-juj71nc5 129 4 S s NN cord-274424-juj71nc5 129 5 protein protein NN cord-274424-juj71nc5 129 6 intracellular intracellular JJ cord-274424-juj71nc5 129 7 transport transport NN cord-274424-juj71nc5 129 8 was be VBD cord-274424-juj71nc5 129 9 compared compare VBN cord-274424-juj71nc5 129 10 by by IN cord-274424-juj71nc5 129 11 measuring measure VBG cord-274424-juj71nc5 129 12 the the DT cord-274424-juj71nc5 129 13 acquisition acquisition NN cord-274424-juj71nc5 129 14 of of IN cord-274424-juj71nc5 129 15 Endo Endo NNP cord-274424-juj71nc5 129 16 H H NNP cord-274424-juj71nc5 129 17 resis- resis- XX cord-274424-juj71nc5 130 1 Note note VB cord-274424-juj71nc5 130 2 . . . cord-274424-juj71nc5 131 1 TGEV TGEV NNP cord-274424-juj71nc5 131 2 neutralizing neutralize VBG cord-274424-juj71nc5 131 3 antibody antibody NN cord-274424-juj71nc5 131 4 was be VBD cord-274424-juj71nc5 131 5 titrated titrate VBN cord-274424-juj71nc5 131 6 by by IN cord-274424-juj71nc5 131 7 a a DT cord-274424-juj71nc5 131 8 50 50 CD cord-274424-juj71nc5 131 9 % % NN cord-274424-juj71nc5 131 10 plaque plaque NN cord-274424-juj71nc5 131 11 reduction reduction NN cord-274424-juj71nc5 131 12 assay assay NN cord-274424-juj71nc5 131 13 and and CC cord-274424-juj71nc5 131 14 expressed express VBD cord-274424-juj71nc5 131 15 as as IN cord-274424-juj71nc5 131 16 the the DT cord-274424-juj71nc5 131 17 reciprocal reciprocal NN cord-274424-juj71nc5 131 18 of of IN cord-274424-juj71nc5 131 19 the the DT cord-274424-juj71nc5 131 20 antiserum antiserum NN cord-274424-juj71nc5 131 21 dilution dilution NN cord-274424-juj71nc5 131 22 . . . cord-274424-juj71nc5 132 1 tance tance NNP cord-274424-juj71nc5 132 2 in in IN cord-274424-juj71nc5 132 3 TGEV- TGEV- NNP cord-274424-juj71nc5 132 4 , , , cord-274424-juj71nc5 132 5 vTS vts FW cord-274424-juj71nc5 132 6 - - HYPH cord-274424-juj71nc5 132 7 I- I- NNP cord-274424-juj71nc5 132 8 , , , cord-274424-juj71nc5 132 9 and and CC cord-274424-juj71nc5 132 10 vTSA-1 vTSA-1 NNP cord-274424-juj71nc5 132 11 -infected -infected JJ cord-274424-juj71nc5 132 12 cells cell NNS cord-274424-juj71nc5 132 13 . . . cord-274424-juj71nc5 133 1 After after IN cord-274424-juj71nc5 133 2 a a DT cord-274424-juj71nc5 133 3 I i NN cord-274424-juj71nc5 133 4 - - HYPH cord-274424-juj71nc5 133 5 hr hr NN cord-274424-juj71nc5 133 6 pulse pulse NN cord-274424-juj71nc5 133 7 , , , cord-274424-juj71nc5 133 8 the the DT cord-274424-juj71nc5 133 9 TGEV TGEV NNP cord-274424-juj71nc5 133 10 S S NNP cord-274424-juj71nc5 133 11 protein protein NN cord-274424-juj71nc5 133 12 consisted consist VBD cord-274424-juj71nc5 133 13 of of IN cord-274424-juj71nc5 133 14 a a DT cord-274424-juj71nc5 133 15 major major JJ cord-274424-juj71nc5 133 16 gp190 gp190 CD cord-274424-juj71nc5 133 17 component component NN cord-274424-juj71nc5 133 18 and and CC cord-274424-juj71nc5 133 19 a a DT cord-274424-juj71nc5 133 20 minor minor JJ cord-274424-juj71nc5 133 21 gp210 gp210 NN cord-274424-juj71nc5 133 22 component component NN cord-274424-juj71nc5 133 23 ( ( -LRB- cord-274424-juj71nc5 133 24 Fig Fig NNP cord-274424-juj71nc5 133 25 . . . cord-274424-juj71nc5 133 26 5A 5a CD cord-274424-juj71nc5 133 27 ) ) -RRB- cord-274424-juj71nc5 133 28 . . . cord-274424-juj71nc5 134 1 After after IN cord-274424-juj71nc5 134 2 a a DT cord-274424-juj71nc5 134 3 1-hr 1-hr CD cord-274424-juj71nc5 134 4 chase chase NN cord-274424-juj71nc5 134 5 , , , cord-274424-juj71nc5 134 6 gpl90 gpl90 NNP cord-274424-juj71nc5 134 7 and and CC cord-274424-juj71nc5 134 8 gp210 gp210 NNP cord-274424-juj71nc5 134 9 were be VBD cord-274424-juj71nc5 134 10 present present JJ cord-274424-juj71nc5 134 11 in in IN cord-274424-juj71nc5 134 12 approximately approximately RB cord-274424-juj71nc5 134 13 equal equal JJ cord-274424-juj71nc5 134 14 proportions proportion NNS cord-274424-juj71nc5 134 15 but but CC cord-274424-juj71nc5 134 16 after after IN cord-274424-juj71nc5 134 17 a a DT cord-274424-juj71nc5 134 18 2-hr 2-hr JJ cord-274424-juj71nc5 134 19 chase chase NN cord-274424-juj71nc5 134 20 , , , cord-274424-juj71nc5 134 21 gp210 gp210 NNP cord-274424-juj71nc5 134 22 was be VBD cord-274424-juj71nc5 134 23 the the DT cord-274424-juj71nc5 134 24 dominant dominant JJ cord-274424-juj71nc5 134 25 S S NNP cord-274424-juj71nc5 134 26 protein protein NN cord-274424-juj71nc5 134 27 component component NN cord-274424-juj71nc5 134 28 and and CC cord-274424-juj71nc5 134 29 the the DT cord-274424-juj71nc5 134 30 majority majority NN cord-274424-juj71nc5 134 31 was be VBD cord-274424-juj71nc5 134 32 Endo Endo NNP cord-274424-juj71nc5 134 33 H H NNP cord-274424-juj71nc5 134 34 resistant resistant JJ cord-274424-juj71nc5 134 35 . . . cord-274424-juj71nc5 135 1 TGEV TGEV NNP cord-274424-juj71nc5 135 2 S S NNP cord-274424-juj71nc5 135 3 protein protein NN cord-274424-juj71nc5 135 4 steadily steadily RB cord-274424-juj71nc5 135 5 became become VBD cord-274424-juj71nc5 135 6 Endo Endo NNP cord-274424-juj71nc5 135 7 H H NNP cord-274424-juj71nc5 135 8 resistant resistant JJ cord-274424-juj71nc5 135 9 with with IN cord-274424-juj71nc5 135 10 time time NN cord-274424-juj71nc5 135 11 such such JJ cord-274424-juj71nc5 135 12 that that IN cord-274424-juj71nc5 135 13 after after IN cord-274424-juj71nc5 135 14 a a DT cord-274424-juj71nc5 135 15 4-hr 4-hr CD cord-274424-juj71nc5 135 16 chase chase NN cord-274424-juj71nc5 136 1 virtually virtually RB cord-274424-juj71nc5 136 2 all all DT cord-274424-juj71nc5 136 3 S S NNP cord-274424-juj71nc5 136 4 protein protein NN cord-274424-juj71nc5 136 5 had have VBD cord-274424-juj71nc5 136 6 been be VBN cord-274424-juj71nc5 136 7 modified modify VBN cord-274424-juj71nc5 136 8 in in IN cord-274424-juj71nc5 136 9 the the DT cord-274424-juj71nc5 136 10 Golgi Golgi NNP cord-274424-juj71nc5 136 11 . . . cord-274424-juj71nc5 137 1 S s NN cord-274424-juj71nc5 137 2 protein protein NN cord-274424-juj71nc5 137 3 was be VBD cord-274424-juj71nc5 137 4 also also RB cord-274424-juj71nc5 137 5 detected detect VBN cord-274424-juj71nc5 137 6 in in IN cord-274424-juj71nc5 137 7 the the DT cord-274424-juj71nc5 137 8 tissue tissue NN cord-274424-juj71nc5 137 9 culture culture NN cord-274424-juj71nc5 137 10 medium medium NN cord-274424-juj71nc5 137 11 of of IN cord-274424-juj71nc5 137 12 TGEV TGEV NNP cord-274424-juj71nc5 137 13 - - HYPH cord-274424-juj71nc5 137 14 infected infect VBN cord-274424-juj71nc5 137 15 cells cell NNS cord-274424-juj71nc5 137 16 after after IN cord-274424-juj71nc5 137 17 a a DT cord-274424-juj71nc5 137 18 1 1 CD cord-274424-juj71nc5 137 19 -hr -hr NN cord-274424-juj71nc5 137 20 chase chase NN cord-274424-juj71nc5 137 21 and and CC cord-274424-juj71nc5 137 22 accumulated accumulate VBN cord-274424-juj71nc5 137 23 steadily steadily RB cord-274424-juj71nc5 137 24 with with IN cord-274424-juj71nc5 137 25 time time NN cord-274424-juj71nc5 137 26 . . . cord-274424-juj71nc5 138 1 Digestion digestion NN cord-274424-juj71nc5 138 2 of of IN cord-274424-juj71nc5 138 3 gp210 gp210 NNP cord-274424-juj71nc5 138 4 from from IN cord-274424-juj71nc5 138 5 extracellular extracellular JJ cord-274424-juj71nc5 138 6 or or CC cord-274424-juj71nc5 138 7 intracellular intracellular JJ cord-274424-juj71nc5 138 8 sources source NNS cord-274424-juj71nc5 138 9 with with IN cord-274424-juj71nc5 138 10 Endo Endo NNP cord-274424-juj71nc5 138 11 H H NNP cord-274424-juj71nc5 138 12 reduced reduce VBD cord-274424-juj71nc5 138 13 the the DT cord-274424-juj71nc5 138 14 size size NN cord-274424-juj71nc5 138 15 of of IN cord-274424-juj71nc5 138 16 the the DT cord-274424-juj71nc5 138 17 S S NNP cord-274424-juj71nc5 138 18 protein protein NN cord-274424-juj71nc5 138 19 to to IN cord-274424-juj71nc5 138 20 M M NNP cord-274424-juj71nc5 138 21 , , , cord-274424-juj71nc5 138 22 205,000 205,000 CD cord-274424-juj71nc5 138 23 . . . cord-274424-juj71nc5 139 1 This this DT cord-274424-juj71nc5 139 2 observation observation NN cord-274424-juj71nc5 139 3 has have VBZ cord-274424-juj71nc5 139 4 also also RB cord-274424-juj71nc5 139 5 been be VBN cord-274424-juj71nc5 139 6 made make VBN cord-274424-juj71nc5 139 7 for for IN cord-274424-juj71nc5 139 8 the the DT cord-274424-juj71nc5 139 9 FIPV FIPV NNP cord-274424-juj71nc5 139 10 S S NNP cord-274424-juj71nc5 139 11 protein protein NN cord-274424-juj71nc5 139 12 from from IN cord-274424-juj71nc5 139 13 FIPV FIPV NNP cord-274424-juj71nc5 139 14 - - HYPH cord-274424-juj71nc5 139 15 infected infect VBN cord-274424-juj71nc5 139 16 feline feline JJ cord-274424-juj71nc5 139 17 cells cell NNS cord-274424-juj71nc5 139 18 ( ( -LRB- cord-274424-juj71nc5 139 19 Vennema Vennema NNP cord-274424-juj71nc5 139 20 eta/. eta/. NNP cord-274424-juj71nc5 139 21 , , , cord-274424-juj71nc5 139 22 1990 1990 CD cord-274424-juj71nc5 139 23 ) ) -RRB- cord-274424-juj71nc5 139 24 and and CC cord-274424-juj71nc5 139 25 suggested suggest VBD cord-274424-juj71nc5 139 26 that that IN cord-274424-juj71nc5 139 27 coronavirus coronavirus NN cord-274424-juj71nc5 139 28 S s NN cord-274424-juj71nc5 139 29 proteins protein NNS cord-274424-juj71nc5 139 30 still still RB cord-274424-juj71nc5 139 31 have have VBP cord-274424-juj71nc5 139 32 high high JJ cord-274424-juj71nc5 139 33 mannose mannose NN cord-274424-juj71nc5 139 34 or or CC cord-274424-juj71nc5 139 35 hybrid hybrid JJ cord-274424-juj71nc5 139 36 oligosaccharide oligosaccharide NN cord-274424-juj71nc5 139 37 structures structure NNS cord-274424-juj71nc5 139 38 following follow VBG cord-274424-juj71nc5 139 39 Golgi Golgi NNP cord-274424-juj71nc5 139 40 processing processing NN cord-274424-juj71nc5 139 41 . . . cord-274424-juj71nc5 140 1 The the DT cord-274424-juj71nc5 140 2 recombinant recombinant JJ cord-274424-juj71nc5 140 3 S s NN cord-274424-juj71nc5 140 4 protein protein NN cord-274424-juj71nc5 140 5 was be VBD cord-274424-juj71nc5 140 6 completely completely RB cord-274424-juj71nc5 140 7 Endo Endo NNP cord-274424-juj71nc5 140 8 H H NNP cord-274424-juj71nc5 140 9 sensitive sensitive JJ cord-274424-juj71nc5 140 10 after after IN cord-274424-juj71nc5 140 11 a a DT cord-274424-juj71nc5 140 12 1-hr 1-hr CD cord-274424-juj71nc5 140 13 pulse pulse NN cord-274424-juj71nc5 140 14 and and CC cord-274424-juj71nc5 140 15 after after IN cord-274424-juj71nc5 140 16 chasing chase VBG cord-274424-juj71nc5 140 17 became become VBD cord-274424-juj71nc5 140 18 partially partially RB cord-274424-juj71nc5 140 19 Endo Endo NNP cord-274424-juj71nc5 140 20 H H NNP cord-274424-juj71nc5 140 21 resistant resistant JJ cord-274424-juj71nc5 140 22 with with IN cord-274424-juj71nc5 140 23 approximately approximately RB cord-274424-juj71nc5 140 24 half half NN cord-274424-juj71nc5 140 25 of of IN cord-274424-juj71nc5 140 26 the the DT cord-274424-juj71nc5 140 27 protein protein NN cord-274424-juj71nc5 140 28 observed observe VBD cord-274424-juj71nc5 140 29 as as IN cord-274424-juj71nc5 140 30 unresolved unresolved JJ cord-274424-juj71nc5 140 31 components component NNS cord-274424-juj71nc5 140 32 of of IN cord-274424-juj71nc5 140 33 high high JJ cord-274424-juj71nc5 140 34 molecular molecular JJ cord-274424-juj71nc5 140 35 weight weight NN cord-274424-juj71nc5 140 36 ( ( -LRB- cord-274424-juj71nc5 140 37 Fig Fig NNP cord-274424-juj71nc5 140 38 . . . cord-274424-juj71nc5 140 39 5B 5b CD cord-274424-juj71nc5 140 40 ) ) -RRB- cord-274424-juj71nc5 140 41 . . . cord-274424-juj71nc5 141 1 Extracellular Extracellular NNP cord-274424-juj71nc5 141 2 S S NNP cord-274424-juj71nc5 141 3 protein protein NN cord-274424-juj71nc5 141 4 was be VBD cord-274424-juj71nc5 141 5 not not RB cord-274424-juj71nc5 141 6 detected detect VBN cord-274424-juj71nc5 141 7 in in IN cord-274424-juj71nc5 141 8 the the DT cord-274424-juj71nc5 141 9 culture culture NN cord-274424-juj71nc5 141 10 medium medium NN cord-274424-juj71nc5 141 11 after after IN cord-274424-juj71nc5 141 12 chasing chase VBG cord-274424-juj71nc5 141 13 for for IN cord-274424-juj71nc5 141 14 4 4 CD cord-274424-juj71nc5 141 15 hr hr NN cord-274424-juj71nc5 141 16 because because IN cord-274424-juj71nc5 141 17 S s NN cord-274424-juj71nc5 141 18 protein protein NN cord-274424-juj71nc5 141 19 transport transport NN cord-274424-juj71nc5 141 20 from from IN cord-274424-juj71nc5 141 21 the the DT cord-274424-juj71nc5 141 22 RER rer NN cord-274424-juj71nc5 141 23 through through IN cord-274424-juj71nc5 141 24 the the DT cord-274424-juj71nc5 141 25 Golgi Golgi NNP cord-274424-juj71nc5 141 26 stack stack NN cord-274424-juj71nc5 141 27 and and CC cord-274424-juj71nc5 141 28 out out IN cord-274424-juj71nc5 141 29 of of IN cord-274424-juj71nc5 141 30 the the DT cord-274424-juj71nc5 141 31 cell cell NN cord-274424-juj71nc5 141 32 was be VBD cord-274424-juj71nc5 141 33 significantly significantly RB cord-274424-juj71nc5 141 34 disrupted disrupt VBN cord-274424-juj71nc5 141 35 in in IN cord-274424-juj71nc5 141 36 the the DT cord-274424-juj71nc5 141 37 absence absence NN cord-274424-juj71nc5 141 38 of of IN cord-274424-juj71nc5 141 39 coronaviral coronaviral JJ cord-274424-juj71nc5 141 40 morphogenesis morphogenesis NN cord-274424-juj71nc5 141 41 . . . cord-274424-juj71nc5 142 1 Infection infection NN cord-274424-juj71nc5 142 2 of of IN cord-274424-juj71nc5 142 3 LLC LLC NNP cord-274424-juj71nc5 142 4 - - HYPH cord-274424-juj71nc5 142 5 PKl PKl NNP cord-274424-juj71nc5 142 6 cells cell NNS cord-274424-juj71nc5 142 7 with with IN cord-274424-juj71nc5 142 8 vTS-1 vTS-1 NNP cord-274424-juj71nc5 142 9 produced produce VBD cord-274424-juj71nc5 142 10 a a DT cord-274424-juj71nc5 142 11 discrete discrete JJ cord-274424-juj71nc5 142 12 Mr Mr NNP cord-274424-juj71nc5 142 13 2 2 CD cord-274424-juj71nc5 142 14 10,000 10,000 CD cord-274424-juj71nc5 142 15 Endo Endo NNP cord-274424-juj71nc5 142 16 H h NN cord-274424-juj71nc5 142 17 - - HYPH cord-274424-juj71nc5 142 18 resistant resistant JJ cord-274424-juj71nc5 142 19 form form NN cord-274424-juj71nc5 142 20 of of IN cord-274424-juj71nc5 142 21 S s NN cord-274424-juj71nc5 142 22 protein protein NN cord-274424-juj71nc5 142 23 ( ( -LRB- cord-274424-juj71nc5 142 24 data datum NNS cord-274424-juj71nc5 142 25 not not RB cord-274424-juj71nc5 142 26 shown show VBN cord-274424-juj71nc5 142 27 ) ) -RRB- cord-274424-juj71nc5 142 28 . . . cord-274424-juj71nc5 143 1 This this DT cord-274424-juj71nc5 143 2 was be VBD cord-274424-juj71nc5 143 3 in in IN cord-274424-juj71nc5 143 4 contrast contrast NN cord-274424-juj71nc5 143 5 to to IN cord-274424-juj71nc5 143 6 the the DT cord-274424-juj71nc5 143 7 partial partial JJ cord-274424-juj71nc5 143 8 Endo Endo NNP cord-274424-juj71nc5 143 9 H H NNP cord-274424-juj71nc5 143 10 - - HYPH cord-274424-juj71nc5 143 11 resistant resistant JJ cord-274424-juj71nc5 143 12 forms form NNS cord-274424-juj71nc5 143 13 observed observe VBN cord-274424-juj71nc5 143 14 in in IN cord-274424-juj71nc5 143 15 vTS-1 vTS-1 NNP cord-274424-juj71nc5 143 16 -infected -infected JJ cord-274424-juj71nc5 143 17 HTK HTK NNP cord-274424-juj71nc5 143 18 - - HYPH cord-274424-juj71nc5 143 19 cells cell NNS cord-274424-juj71nc5 143 20 , , , cord-274424-juj71nc5 143 21 implying imply VBG cord-274424-juj71nc5 143 22 that that IN cord-274424-juj71nc5 143 23 the the DT cord-274424-juj71nc5 143 24 TGEV TGEV NNP cord-274424-juj71nc5 143 25 S S NNP cord-274424-juj71nc5 143 26 protein protein NN cord-274424-juj71nc5 143 27 may may MD cord-274424-juj71nc5 143 28 be be VB cord-274424-juj71nc5 143 29 post post JJ cord-274424-juj71nc5 143 30 - - JJ cord-274424-juj71nc5 143 31 translationally translationally RB cord-274424-juj71nc5 143 32 modified modify VBN cord-274424-juj71nc5 143 33 to to IN cord-274424-juj71nc5 143 34 a a DT cord-274424-juj71nc5 143 35 different different JJ cord-274424-juj71nc5 143 36 extent extent NN cord-274424-juj71nc5 143 37 depending depend VBG cord-274424-juj71nc5 143 38 on on IN cord-274424-juj71nc5 143 39 the the DT cord-274424-juj71nc5 143 40 origin origin NN cord-274424-juj71nc5 143 41 of of IN cord-274424-juj71nc5 143 42 the the DT cord-274424-juj71nc5 143 43 cell cell NN cord-274424-juj71nc5 143 44 line line NN cord-274424-juj71nc5 143 45 . . . cord-274424-juj71nc5 144 1 The the DT cord-274424-juj71nc5 144 2 SA SA NNP cord-274424-juj71nc5 144 3 protein protein NN cord-274424-juj71nc5 144 4 remained remain VBD cord-274424-juj71nc5 144 5 completely completely RB cord-274424-juj71nc5 144 6 Endo Endo NNP cord-274424-juj71nc5 144 7 H H NNP cord-274424-juj71nc5 144 8 sensitive sensitive JJ cord-274424-juj71nc5 144 9 , , , cord-274424-juj71nc5 144 10 even even RB cord-274424-juj71nc5 144 11 after after IN cord-274424-juj71nc5 144 12 a a DT cord-274424-juj71nc5 144 13 4-hr 4-hr CD cord-274424-juj71nc5 144 14 chase chase NN cord-274424-juj71nc5 144 15 , , , cord-274424-juj71nc5 144 16 demonstrating demonstrate VBG cord-274424-juj71nc5 144 17 that that IN cord-274424-juj71nc5 144 18 the the DT cord-274424-juj71nc5 144 19 truncated truncated JJ cord-274424-juj71nc5 144 20 S S NNP cord-274424-juj71nc5 144 21 protein protein NN cord-274424-juj71nc5 144 22 was be VBD cord-274424-juj71nc5 144 23 glycosylated glycosylate VBN cord-274424-juj71nc5 144 24 with with IN cord-274424-juj71nc5 144 25 high high JJ cord-274424-juj71nc5 144 26 mannose mannose NN cord-274424-juj71nc5 144 27 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 144 28 only only RB cord-274424-juj71nc5 144 29 and and CC cord-274424-juj71nc5 144 30 was be VBD cord-274424-juj71nc5 144 31 not not RB cord-274424-juj71nc5 144 32 subject subject JJ cord-274424-juj71nc5 144 33 to to IN cord-274424-juj71nc5 144 34 Golgi golgi NN cord-274424-juj71nc5 144 35 - - HYPH cord-274424-juj71nc5 144 36 specific specific JJ cord-274424-juj71nc5 144 37 modifications modification NNS cord-274424-juj71nc5 144 38 ( ( -LRB- cord-274424-juj71nc5 144 39 Fig fig NN cord-274424-juj71nc5 144 40 . . . cord-274424-juj71nc5 144 41 5B 5b CD cord-274424-juj71nc5 144 42 ) ) -RRB- cord-274424-juj71nc5 144 43 . . . cord-274424-juj71nc5 145 1 The the DT cord-274424-juj71nc5 145 2 cellular cellular JJ cord-274424-juj71nc5 145 3 location location NN cord-274424-juj71nc5 145 4 of of IN cord-274424-juj71nc5 145 5 the the DT cord-274424-juj71nc5 145 6 S S NNP cord-274424-juj71nc5 145 7 and and CC cord-274424-juj71nc5 145 8 SA SA NNP cord-274424-juj71nc5 145 9 protein protein NN cord-274424-juj71nc5 145 10 in in IN cord-274424-juj71nc5 145 11 rVVinfected rvvinfected JJ cord-274424-juj71nc5 145 12 cells cell NNS cord-274424-juj71nc5 145 13 was be VBD cord-274424-juj71nc5 145 14 analyzed analyze VBN cord-274424-juj71nc5 145 15 by by IN cord-274424-juj71nc5 145 16 indirect indirect JJ cord-274424-juj71nc5 145 17 immunofluorescence immunofluorescence NN cord-274424-juj71nc5 145 18 microscopy microscopy NN cord-274424-juj71nc5 145 19 ( ( -LRB- cord-274424-juj71nc5 145 20 Fig Fig NNP cord-274424-juj71nc5 145 21 . . . cord-274424-juj71nc5 145 22 6 6 CD cord-274424-juj71nc5 145 23 ) ) -RRB- cord-274424-juj71nc5 145 24 . . . cord-274424-juj71nc5 146 1 Acetone acetone NN cord-274424-juj71nc5 146 2 - - HYPH cord-274424-juj71nc5 146 3 fixed fix VBN cord-274424-juj71nc5 146 4 TGEV TGEV NNP cord-274424-juj71nc5 146 5 - - HYPH cord-274424-juj71nc5 146 6 infected infect VBN cord-274424-juj71nc5 146 7 LLC LLC NNP cord-274424-juj71nc5 146 8 - - HYPH cord-274424-juj71nc5 146 9 PKl PKl NNP cord-274424-juj71nc5 146 10 cells cell NNS cord-274424-juj71nc5 146 11 , , , cord-274424-juj71nc5 146 12 probed probe VBN cord-274424-juj71nc5 146 13 with with IN cord-274424-juj71nc5 146 14 anti anti JJ cord-274424-juj71nc5 146 15 - - JJ cord-274424-juj71nc5 146 16 S s JJ cord-274424-juj71nc5 146 17 - - HYPH cord-274424-juj71nc5 146 18 specific specific JJ cord-274424-juj71nc5 146 19 serum serum NN cord-274424-juj71nc5 146 20 , , , cord-274424-juj71nc5 146 21 had have VBD cord-274424-juj71nc5 146 22 a a DT cord-274424-juj71nc5 146 23 granular granular JJ cord-274424-juj71nc5 146 24 appearance appearance NN cord-274424-juj71nc5 146 25 with with IN cord-274424-juj71nc5 146 26 occasional occasional JJ cord-274424-juj71nc5 146 27 bright bright JJ cord-274424-juj71nc5 146 28 accumulations accumulation NNS cord-274424-juj71nc5 146 29 in in IN cord-274424-juj71nc5 146 30 localized localized JJ cord-274424-juj71nc5 146 31 parts part NNS cord-274424-juj71nc5 146 32 of of IN cord-274424-juj71nc5 146 33 the the DT cord-274424-juj71nc5 146 34 cell cell NN cord-274424-juj71nc5 146 35 ( ( -LRB- cord-274424-juj71nc5 146 36 data datum NNS cord-274424-juj71nc5 146 37 not not RB cord-274424-juj71nc5 146 38 shown show VBN cord-274424-juj71nc5 146 39 ) ) -RRB- cord-274424-juj71nc5 146 40 . . . cord-274424-juj71nc5 147 1 In in IN cord-274424-juj71nc5 147 2 vTS vTS NNP cord-274424-juj71nc5 147 3 - - HYPH cord-274424-juj71nc5 147 4 l l NN cord-274424-juj71nc5 147 5 - - HYPH cord-274424-juj71nc5 147 6 infected infect VBN cord-274424-juj71nc5 147 7 HTK- HTK- NNP cord-274424-juj71nc5 147 8 ( ( -LRB- cord-274424-juj71nc5 147 9 Fig fig NN cord-274424-juj71nc5 147 10 . . . cord-274424-juj71nc5 147 11 6A 6a CD cord-274424-juj71nc5 147 12 ) ) -RRB- cord-274424-juj71nc5 147 13 and and CC cord-274424-juj71nc5 147 14 LLC LLC NNP cord-274424-juj71nc5 147 15 - - HYPH cord-274424-juj71nc5 147 16 PKl PKl NNP cord-274424-juj71nc5 147 17 ( ( -LRB- cord-274424-juj71nc5 147 18 Fig Fig NNP cord-274424-juj71nc5 147 19 . . . cord-274424-juj71nc5 147 20 6C 6c NN cord-274424-juj71nc5 147 21 ) ) -RRB- cord-274424-juj71nc5 148 1 an an DT cord-274424-juj71nc5 148 2 intracellular intracellular JJ cord-274424-juj71nc5 148 3 compartment compartment NN cord-274424-juj71nc5 148 4 in in IN cord-274424-juj71nc5 148 5 all all DT cord-274424-juj71nc5 148 6 infected infected JJ cord-274424-juj71nc5 148 7 cells cell NNS cord-274424-juj71nc5 148 8 ( ( -LRB- cord-274424-juj71nc5 148 9 represented represent VBN cord-274424-juj71nc5 148 10 with with IN cord-274424-juj71nc5 148 11 arrows arrow NNS cord-274424-juj71nc5 148 12 ) ) -RRB- cord-274424-juj71nc5 148 13 , , , cord-274424-juj71nc5 148 14 while while IN cord-274424-juj71nc5 148 15 vTSA-1 vTSA-1 , cord-274424-juj71nc5 148 16 -infected -infected JJ cord-274424-juj71nc5 148 17 cells cell NNS cord-274424-juj71nc5 148 18 had have VBD cord-274424-juj71nc5 148 19 a a DT cord-274424-juj71nc5 148 20 uniform uniform JJ cord-274424-juj71nc5 148 21 distribution distribution NN cord-274424-juj71nc5 148 22 of of IN cord-274424-juj71nc5 148 23 SA SA NNP cord-274424-juj71nc5 148 24 protein protein NN cord-274424-juj71nc5 148 25 throughout throughout IN cord-274424-juj71nc5 148 26 the the DT cord-274424-juj71nc5 148 27 cytoplasm cytoplasm NN cord-274424-juj71nc5 149 1 ( ( -LRB- cord-274424-juj71nc5 149 2 Fig Fig NNP cord-274424-juj71nc5 149 3 . . . cord-274424-juj71nc5 149 4 6B 6b CD cord-274424-juj71nc5 149 5 and and CC cord-274424-juj71nc5 149 6 6D 6D NNP cord-274424-juj71nc5 149 7 ) ) -RRB- cord-274424-juj71nc5 149 8 . . . cord-274424-juj71nc5 150 1 Recombinant Recombinant NNP cord-274424-juj71nc5 150 2 S S NNP cord-274424-juj71nc5 150 3 protein protein NN cord-274424-juj71nc5 150 4 was be VBD cord-274424-juj71nc5 150 5 also also RB cord-274424-juj71nc5 150 6 observed observe VBN cord-274424-juj71nc5 150 7 on on IN cord-274424-juj71nc5 150 8 the the DT cord-274424-juj71nc5 150 9 cell cell NN cord-274424-juj71nc5 150 10 surface surface NN cord-274424-juj71nc5 150 11 of of IN cord-274424-juj71nc5 150 12 unfixed unfixed JJ cord-274424-juj71nc5 150 13 infected infected JJ cord-274424-juj71nc5 150 14 HTK HTK NNP cord-274424-juj71nc5 150 15 - - HYPH cord-274424-juj71nc5 150 16 cells cell NNS cord-274424-juj71nc5 150 17 ( ( -LRB- cord-274424-juj71nc5 150 18 Fig Fig NNP cord-274424-juj71nc5 150 19 . . . cord-274424-juj71nc5 150 20 6E 6E NNP cord-274424-juj71nc5 150 21 ) ) -RRB- cord-274424-juj71nc5 150 22 , , , cord-274424-juj71nc5 150 23 unlike unlike IN cord-274424-juj71nc5 150 24 SA SA NNP cord-274424-juj71nc5 150 25 protein protein NN cord-274424-juj71nc5 150 26 ( ( -LRB- cord-274424-juj71nc5 150 27 Fig Fig NNP cord-274424-juj71nc5 150 28 . . . cord-274424-juj71nc5 150 29 6F 6F NNP cord-274424-juj71nc5 150 30 ) ) -RRB- cord-274424-juj71nc5 150 31 , , , cord-274424-juj71nc5 150 32 demonstrating demonstrate VBG cord-274424-juj71nc5 150 33 that that IN cord-274424-juj71nc5 150 34 the the DT cord-274424-juj71nc5 150 35 full full JJ cord-274424-juj71nc5 150 36 - - HYPH cord-274424-juj71nc5 150 37 length length NN cord-274424-juj71nc5 150 38 protein protein NN cord-274424-juj71nc5 150 39 has have VBZ cord-274424-juj71nc5 150 40 all all PDT cord-274424-juj71nc5 150 41 the the DT cord-274424-juj71nc5 150 42 intrinsic intrinsic JJ cord-274424-juj71nc5 150 43 properties property NNS cord-274424-juj71nc5 150 44 for for IN cord-274424-juj71nc5 150 45 cell cell NN cord-274424-juj71nc5 150 46 surface surface NN cord-274424-juj71nc5 150 47 transport transport NN cord-274424-juj71nc5 150 48 and and CC cord-274424-juj71nc5 150 49 does do VBZ cord-274424-juj71nc5 150 50 not not RB cord-274424-juj71nc5 150 51 require require VB cord-274424-juj71nc5 150 52 the the DT cord-274424-juj71nc5 150 53 cooperative cooperative JJ cord-274424-juj71nc5 150 54 effect effect NN cord-274424-juj71nc5 150 55 of of IN cord-274424-juj71nc5 150 56 other other JJ cord-274424-juj71nc5 150 57 TGEV TGEV NNP cord-274424-juj71nc5 150 58 proteins protein NNS cord-274424-juj71nc5 150 59 . . . cord-274424-juj71nc5 151 1 The the DT cord-274424-juj71nc5 151 2 possibility possibility NN cord-274424-juj71nc5 151 3 that that IN cord-274424-juj71nc5 151 4 soluble soluble JJ cord-274424-juj71nc5 151 5 S S NNP cord-274424-juj71nc5 151 6 protein protein NN cord-274424-juj71nc5 151 7 released release VBN cord-274424-juj71nc5 151 8 from from IN cord-274424-juj71nc5 151 9 human human JJ cord-274424-juj71nc5 151 10 cells cell NNS cord-274424-juj71nc5 151 11 may may MD cord-274424-juj71nc5 151 12 bind bind VB cord-274424-juj71nc5 151 13 back back RB cord-274424-juj71nc5 151 14 to to IN cord-274424-juj71nc5 151 15 cell cell NN cord-274424-juj71nc5 151 16 surface surface NN cord-274424-juj71nc5 151 17 receptors receptor NNS cord-274424-juj71nc5 151 18 and and CC cord-274424-juj71nc5 151 19 give give VB cord-274424-juj71nc5 151 20 the the DT cord-274424-juj71nc5 151 21 appearance appearance NN cord-274424-juj71nc5 151 22 of of IN cord-274424-juj71nc5 151 23 cell cell NN cord-274424-juj71nc5 151 24 surface surface NN cord-274424-juj71nc5 151 25 fluorescence fluorescence NN cord-274424-juj71nc5 151 26 can can MD cord-274424-juj71nc5 151 27 be be VB cord-274424-juj71nc5 151 28 disregarded disregard VBN cord-274424-juj71nc5 151 29 as as IN cord-274424-juj71nc5 151 30 TGEV TGEV NNP cord-274424-juj71nc5 151 31 S S NNP cord-274424-juj71nc5 151 32 protein protein NN cord-274424-juj71nc5 151 33 only only RB cord-274424-juj71nc5 151 34 binds bind VBZ cord-274424-juj71nc5 151 35 porcine porcine JJ cord-274424-juj71nc5 151 36 cell cell NN cord-274424-juj71nc5 151 37 surface surface NN cord-274424-juj71nc5 151 38 receptors receptor NNS cord-274424-juj71nc5 151 39 . . . cord-274424-juj71nc5 152 1 Studies study NNS cord-274424-juj71nc5 152 2 described describe VBN cord-274424-juj71nc5 152 3 in in IN cord-274424-juj71nc5 152 4 this this DT cord-274424-juj71nc5 152 5 paper paper NN cord-274424-juj71nc5 152 6 with with IN cord-274424-juj71nc5 152 7 TGEV TGEV NNP cord-274424-juj71nc5 152 8 recombinant recombinant JJ cord-274424-juj71nc5 152 9 S S NNP cord-274424-juj71nc5 152 10 protein protein NN cord-274424-juj71nc5 152 11 have have VBP cord-274424-juj71nc5 152 12 shown show VBN cord-274424-juj71nc5 152 13 that that IN cord-274424-juj71nc5 152 14 it -PRON- PRP cord-274424-juj71nc5 152 15 is be VBZ cord-274424-juj71nc5 152 16 an an DT cord-274424-juj71nc5 152 17 N n CD cord-274424-juj71nc5 152 18 - - HYPH cord-274424-juj71nc5 152 19 linked link VBN cord-274424-juj71nc5 152 20 glyco glyco NNP cord-274424-juj71nc5 152 21 - - HYPH cord-274424-juj71nc5 152 22 protein protein NN cord-274424-juj71nc5 152 23 with with IN cord-274424-juj71nc5 152 24 a a DT cord-274424-juj71nc5 152 25 M M NNP cord-274424-juj71nc5 152 26 , , , cord-274424-juj71nc5 152 27 190 190 CD cord-274424-juj71nc5 152 28 - - SYM cord-274424-juj71nc5 152 29 210,000 210,000 CD cord-274424-juj71nc5 152 30 . . . cord-274424-juj71nc5 153 1 Mice mouse NNS cord-274424-juj71nc5 153 2 inoculated inoculate VBD cord-274424-juj71nc5 153 3 with with IN cord-274424-juj71nc5 153 4 two two CD cord-274424-juj71nc5 153 5 separate separate JJ cord-274424-juj71nc5 153 6 clones clone NNS cord-274424-juj71nc5 153 7 of of IN cord-274424-juj71nc5 153 8 vTS vts NN cord-274424-juj71nc5 153 9 demonstrated demonstrate VBD cord-274424-juj71nc5 153 10 that that IN cord-274424-juj71nc5 153 11 the the DT cord-274424-juj71nc5 153 12 recombinant recombinant JJ cord-274424-juj71nc5 153 13 S S NNP cord-274424-juj71nc5 153 14 protein protein NN cord-274424-juj71nc5 153 15 was be VBD cord-274424-juj71nc5 153 16 immunogenic immunogenic JJ cord-274424-juj71nc5 153 17 and and CC cord-274424-juj71nc5 153 18 elicited elicit VBD cord-274424-juj71nc5 153 19 TGEV TGEV NNP cord-274424-juj71nc5 153 20 neutralizing neutralize VBG cord-274424-juj71nc5 153 21 antibodies antibody NNS cord-274424-juj71nc5 153 22 . . . cord-274424-juj71nc5 154 1 This this DT cord-274424-juj71nc5 154 2 was be VBD cord-274424-juj71nc5 154 3 in in IN cord-274424-juj71nc5 154 4 contrast contrast NN cord-274424-juj71nc5 154 5 to to IN cord-274424-juj71nc5 154 6 previous previous JJ cord-274424-juj71nc5 154 7 studies study NNS cord-274424-juj71nc5 154 8 with with IN cord-274424-juj71nc5 154 9 TGEV TGEV NNP cord-274424-juj71nc5 154 10 N N NNP cord-274424-juj71nc5 154 11 and and CC cord-274424-juj71nc5 154 12 M M NNP cord-274424-juj71nc5 154 13 proteins protein NNS cord-274424-juj71nc5 154 14 expressed express VBN cord-274424-juj71nc5 154 15 by by IN cord-274424-juj71nc5 154 16 rVVs rvvs FW cord-274424-juj71nc5 154 17 that that WDT cord-274424-juj71nc5 154 18 induced induce VBD cord-274424-juj71nc5 154 19 an an DT cord-274424-juj71nc5 154 20 immune immune JJ cord-274424-juj71nc5 154 21 response response NN cord-274424-juj71nc5 154 22 but but CC cord-274424-juj71nc5 154 23 no no DT cord-274424-juj71nc5 154 24 neutralizing neutralize VBG cord-274424-juj71nc5 154 25 antibodies antibody NNS cord-274424-juj71nc5 154 26 . . . cord-274424-juj71nc5 155 1 The the DT cord-274424-juj71nc5 155 2 TGEV TGEV NNP cord-274424-juj71nc5 155 3 S S NNP cord-274424-juj71nc5 155 4 protein protein NN cord-274424-juj71nc5 155 5 neutralizing neutralize VBG cord-274424-juj71nc5 155 6 epitopes epitope NNS cord-274424-juj71nc5 155 7 and and CC cord-274424-juj71nc5 155 8 major major JJ cord-274424-juj71nc5 155 9 antigenic antigenic JJ cord-274424-juj71nc5 155 10 sites site NNS cord-274424-juj71nc5 155 11 were be VBD cord-274424-juj71nc5 155 12 retained retain VBN cord-274424-juj71nc5 155 13 by by IN cord-274424-juj71nc5 155 14 the the DT cord-274424-juj71nc5 155 15 SA SA NNP cord-274424-juj71nc5 155 16 protein protein NN cord-274424-juj71nc5 155 17 but but CC cord-274424-juj71nc5 155 18 this this DT cord-274424-juj71nc5 155 19 protein protein NN cord-274424-juj71nc5 155 20 was be VBD cord-274424-juj71nc5 155 21 not not RB cord-274424-juj71nc5 155 22 transported transport VBN cord-274424-juj71nc5 155 23 to to IN cord-274424-juj71nc5 155 24 the the DT cord-274424-juj71nc5 155 25 plasma plasma NN cord-274424-juj71nc5 155 26 membrane membrane NN cord-274424-juj71nc5 155 27 or or CC cord-274424-juj71nc5 155 28 through through IN cord-274424-juj71nc5 155 29 the the DT cord-274424-juj71nc5 155 30 Golgi Golgi NNP cord-274424-juj71nc5 155 31 stack stack NN cord-274424-juj71nc5 155 32 and and CC cord-274424-juj71nc5 155 33 must must MD cord-274424-juj71nc5 155 34 therefore therefore RB cord-274424-juj71nc5 155 35 be be VB cord-274424-juj71nc5 155 36 accumulated accumulate VBN cord-274424-juj71nc5 155 37 in in IN cord-274424-juj71nc5 155 38 the the DT cord-274424-juj71nc5 155 39 endoplasmic endoplasmic NN cord-274424-juj71nc5 155 40 reticulum reticulum NN cord-274424-juj71nc5 155 41 . . . cord-274424-juj71nc5 156 1 Hu Hu NNP cord-274424-juj71nc5 156 2 et et FW cord-274424-juj71nc5 156 3 al al NNP cord-274424-juj71nc5 156 4 . . . cord-274424-juj71nc5 157 1 ( ( -LRB- cord-274424-juj71nc5 157 2 1984 1984 CD cord-274424-juj71nc5 157 3 ) ) -RRB- cord-274424-juj71nc5 157 4 constructed construct VBD cord-274424-juj71nc5 157 5 an an DT cord-274424-juj71nc5 157 6 incomplete incomplete JJ cord-274424-juj71nc5 157 7 TGEV TGEV NNP cord-274424-juj71nc5 157 8 S s NN cord-274424-juj71nc5 157 9 protein protein NN cord-274424-juj71nc5 157 10 gene gene NN cord-274424-juj71nc5 157 11 that that WDT cord-274424-juj71nc5 157 12 initiated initiate VBD cord-274424-juj71nc5 157 13 8 8 CD cord-274424-juj71nc5 157 14 bp bp NNP cord-274424-juj71nc5 157 15 downstream downstream NN cord-274424-juj71nc5 157 16 of of IN cord-274424-juj71nc5 157 17 a a DT cord-274424-juj71nc5 157 18 Hpal Hpal NNP cord-274424-juj71nc5 157 19 site site NN cord-274424-juj71nc5 157 20 but but CC cord-274424-juj71nc5 157 21 ended end VBD cord-274424-juj71nc5 157 22 80 80 CD cord-274424-juj71nc5 157 23 bp bp NNS cord-274424-juj71nc5 157 24 upstream upstream RB cord-274424-juj71nc5 157 25 of of IN cord-274424-juj71nc5 157 26 a a DT cord-274424-juj71nc5 157 27 Xbal Xbal NNP cord-274424-juj71nc5 157 28 site site NN cord-274424-juj71nc5 157 29 . . . cord-274424-juj71nc5 158 1 This this DT cord-274424-juj71nc5 158 2 gene gene NN cord-274424-juj71nc5 158 3 construct construct VBZ cord-274424-juj71nc5 158 4 expressed express VBN cord-274424-juj71nc5 158 5 an an DT cord-274424-juj71nc5 158 6 Endo Endo NNP cord-274424-juj71nc5 158 7 H H NNP cord-274424-juj71nc5 158 8 sensitive sensitive JJ cord-274424-juj71nc5 158 9 M M NNP cord-274424-juj71nc5 158 10 , , , cord-274424-juj71nc5 158 11 180,000 180,000 CD cord-274424-juj71nc5 158 12 polypeptide polypeptide NN cord-274424-juj71nc5 158 13 that that WDT cord-274424-juj71nc5 158 14 was be VBD cord-274424-juj71nc5 158 15 not not RB cord-274424-juj71nc5 158 16 transported transport VBN cord-274424-juj71nc5 158 17 to to IN cord-274424-juj71nc5 158 18 the the DT cord-274424-juj71nc5 158 19 cell cell NN cord-274424-juj71nc5 158 20 surface surface NN cord-274424-juj71nc5 158 21 in in IN cord-274424-juj71nc5 158 22 rVV rvv RB cord-274424-juj71nc5 158 23 - - HYPH cord-274424-juj71nc5 158 24 infected infect VBN cord-274424-juj71nc5 158 25 cells cell NNS cord-274424-juj71nc5 158 26 ( ( -LRB- cord-274424-juj71nc5 159 1 Hu Hu NNP cord-274424-juj71nc5 159 2 et et FW cord-274424-juj71nc5 159 3 al al NNP cord-274424-juj71nc5 159 4 . . NNP cord-274424-juj71nc5 159 5 , , , cord-274424-juj71nc5 159 6 1985 1985 CD cord-274424-juj71nc5 159 7 ) ) -RRB- cord-274424-juj71nc5 159 8 . . . cord-274424-juj71nc5 160 1 Sequence sequence NN cord-274424-juj71nc5 160 2 analysis analysis NN cord-274424-juj71nc5 160 3 of of IN cord-274424-juj71nc5 160 4 the the DT cord-274424-juj71nc5 160 5 TGEV TGEV NNP cord-274424-juj71nc5 160 6 Purdue Purdue NNP cord-274424-juj71nc5 160 7 - - HYPH cord-274424-juj71nc5 160 8 l l NNP cord-274424-juj71nc5 160 9 15 15 CD cord-274424-juj71nc5 160 10 ( ( -LRB- cord-274424-juj71nc5 160 11 Jacobs Jacobs NNP cord-274424-juj71nc5 160 12 et et NNP cord-274424-juj71nc5 160 13 al al NNP cord-274424-juj71nc5 160 14 . . NNP cord-274424-juj71nc5 160 15 , , , cord-274424-juj71nc5 160 16 1987 1987 CD cord-274424-juj71nc5 160 17 ; ; : cord-274424-juj71nc5 160 18 Rasschaet Rasschaet NNP cord-274424-juj71nc5 160 19 - - HYPH cord-274424-juj71nc5 160 20 t t NNP cord-274424-juj71nc5 160 21 et et FW cord-274424-juj71nc5 160 22 al al NNP cord-274424-juj71nc5 160 23 . . NNP cord-274424-juj71nc5 160 24 , , , cord-274424-juj71nc5 160 25 1987 1987 CD cord-274424-juj71nc5 160 26 ) ) -RRB- cord-274424-juj71nc5 160 27 and and CC cord-274424-juj71nc5 160 28 FS772/70 FS772/70 NNP cord-274424-juj71nc5 160 29 strains strain NNS cord-274424-juj71nc5 160 30 demonstrated demonstrate VBD cord-274424-juj71nc5 160 31 that that IN cord-274424-juj71nc5 160 32 the the DT cord-274424-juj71nc5 160 33 Xbal Xbal NNP cord-274424-juj71nc5 160 34 site site NN cord-274424-juj71nc5 160 35 was be VBD cord-274424-juj71nc5 160 36 3776 3776 CD cord-274424-juj71nc5 160 37 bp bp NNP cord-274424-juj71nc5 160 38 from from IN cord-274424-juj71nc5 160 39 the the DT cord-274424-juj71nc5 160 40 S S NNP cord-274424-juj71nc5 160 41 protein protein NN cord-274424-juj71nc5 160 42 initiation initiation NN cord-274424-juj71nc5 160 43 codon codon NN cord-274424-juj71nc5 160 44 and and CC cord-274424-juj71nc5 160 45 within within IN cord-274424-juj71nc5 160 46 an an DT cord-274424-juj71nc5 160 47 ORF ORF NNP cord-274424-juj71nc5 160 48 of of IN cord-274424-juj71nc5 160 49 4350 4350 CD cord-274424-juj71nc5 160 50 bp bp NNP cord-274424-juj71nc5 160 51 , , , cord-274424-juj71nc5 160 52 implying imply VBG cord-274424-juj71nc5 160 53 that that IN cord-274424-juj71nc5 160 54 the the DT cord-274424-juj71nc5 160 55 S S NNP cord-274424-juj71nc5 160 56 gene gene NN cord-274424-juj71nc5 160 57 product product NN cord-274424-juj71nc5 160 58 expressed express VBN cord-274424-juj71nc5 160 59 by by IN cord-274424-juj71nc5 160 60 Hu Hu NNP cord-274424-juj71nc5 160 61 et et NNP cord-274424-juj71nc5 160 62 a/. a/. NNP cord-274424-juj71nc5 160 63 ( ( -LRB- cord-274424-juj71nc5 160 64 1985 1985 CD cord-274424-juj71nc5 160 65 ) ) -RRB- cord-274424-juj71nc5 160 66 was be VBD cord-274424-juj71nc5 160 67 a a DT cord-274424-juj71nc5 160 68 truncated truncated JJ cord-274424-juj71nc5 160 69 protein protein NN cord-274424-juj71nc5 160 70 . . . cord-274424-juj71nc5 161 1 Coronavirus Coronavirus NNP cord-274424-juj71nc5 161 2 S S NNP cord-274424-juj71nc5 161 3 proteins protein NNS cord-274424-juj71nc5 161 4 are be VBP cord-274424-juj71nc5 161 5 known know VBN cord-274424-juj71nc5 161 6 to to TO cord-274424-juj71nc5 161 7 be be VB cord-274424-juj71nc5 161 8 highly highly RB cord-274424-juj71nc5 161 9 glycosylated glycosylate VBN cord-274424-juj71nc5 161 10 with with IN cord-274424-juj71nc5 161 11 N n NN cord-274424-juj71nc5 161 12 - - HYPH cord-274424-juj71nc5 161 13 linked link VBN cord-274424-juj71nc5 161 14 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 161 15 and and CC cord-274424-juj71nc5 161 16 undergo undergo VB cord-274424-juj71nc5 161 17 modification modification NN cord-274424-juj71nc5 161 18 with with IN cord-274424-juj71nc5 161 19 complex complex JJ cord-274424-juj71nc5 161 20 sugars sugar NNS cord-274424-juj71nc5 161 21 at at IN cord-274424-juj71nc5 161 22 the the DT cord-274424-juj71nc5 161 23 medial medial JJ cord-274424-juj71nc5 161 24 compartment compartment NN cord-274424-juj71nc5 161 25 of of IN cord-274424-juj71nc5 161 26 the the DT cord-274424-juj71nc5 161 27 Golgi Golgi NNP cord-274424-juj71nc5 161 28 stack stack NN cord-274424-juj71nc5 161 29 during during IN cord-274424-juj71nc5 161 30 virus virus NN cord-274424-juj71nc5 161 31 maturation maturation NN cord-274424-juj71nc5 161 32 ( ( -LRB- cord-274424-juj71nc5 161 33 Niemann Niemann NNP cord-274424-juj71nc5 161 34 et et NNP cord-274424-juj71nc5 161 35 al al NNP cord-274424-juj71nc5 161 36 . . NNP cord-274424-juj71nc5 161 37 , , , cord-274424-juj71nc5 161 38 1982 1982 CD cord-274424-juj71nc5 161 39 ) ) -RRB- cord-274424-juj71nc5 161 40 . . . cord-274424-juj71nc5 162 1 Simple simple JJ cord-274424-juj71nc5 162 2 high high JJ cord-274424-juj71nc5 162 3 mannose mannose NN cord-274424-juj71nc5 162 4 and and CC cord-274424-juj71nc5 162 5 hybrid hybrid JJ cord-274424-juj71nc5 162 6 structures structure NNS cord-274424-juj71nc5 162 7 can can MD cord-274424-juj71nc5 162 8 be be VB cord-274424-juj71nc5 162 9 removed remove VBN cord-274424-juj71nc5 162 10 from from IN cord-274424-juj71nc5 162 11 glycoproteins glycoprotein NNS cord-274424-juj71nc5 162 12 by by IN cord-274424-juj71nc5 162 13 digestion digestion NN cord-274424-juj71nc5 162 14 with with IN cord-274424-juj71nc5 162 15 Endo Endo NNP cord-274424-juj71nc5 162 16 H H NNP cord-274424-juj71nc5 162 17 but but CC cord-274424-juj71nc5 162 18 glycoproteins glycoprotein NNS cord-274424-juj71nc5 162 19 modified modify VBN cord-274424-juj71nc5 162 20 with with IN cord-274424-juj71nc5 162 21 complex complex JJ cord-274424-juj71nc5 162 22 sugar sugar NN cord-274424-juj71nc5 162 23 structures structure NNS cord-274424-juj71nc5 162 24 are be VBP cord-274424-juj71nc5 162 25 resistant resistant JJ cord-274424-juj71nc5 162 26 to to IN cord-274424-juj71nc5 162 27 cleavage cleavage NN cord-274424-juj71nc5 162 28 with with IN cord-274424-juj71nc5 162 29 Endo Endo NNP cord-274424-juj71nc5 162 30 H H NNP cord-274424-juj71nc5 162 31 ( ( -LRB- cord-274424-juj71nc5 162 32 Hubbard Hubbard NNP cord-274424-juj71nc5 162 33 and and CC cord-274424-juj71nc5 162 34 Ivatt Ivatt NNP cord-274424-juj71nc5 162 35 , , , cord-274424-juj71nc5 162 36 1981 1981 CD cord-274424-juj71nc5 162 37 ; ; : cord-274424-juj71nc5 162 38 Dunphy Dunphy NNP cord-274424-juj71nc5 162 39 and and CC cord-274424-juj71nc5 162 40 Rothman Rothman NNP cord-274424-juj71nc5 162 41 , , , cord-274424-juj71nc5 162 42 1985 1985 CD cord-274424-juj71nc5 162 43 ) ) -RRB- cord-274424-juj71nc5 162 44 . . . cord-274424-juj71nc5 163 1 The the DT cord-274424-juj71nc5 163 2 rVV rvv CD cord-274424-juj71nc5 163 3 vTS-1 vTS-1 NNP cord-274424-juj71nc5 163 4 produced produce VBD cord-274424-juj71nc5 163 5 two two CD cord-274424-juj71nc5 163 6 S s NN cord-274424-juj71nc5 163 7 protein protein NN cord-274424-juj71nc5 163 8 species specie NNS cord-274424-juj71nc5 163 9 in in IN cord-274424-juj71nc5 163 10 human human JJ cord-274424-juj71nc5 163 11 cells cell NNS cord-274424-juj71nc5 163 12 with with IN cord-274424-juj71nc5 163 13 different different JJ cord-274424-juj71nc5 163 14 oligosaccharide oligosaccharide NN cord-274424-juj71nc5 163 15 composition composition NN cord-274424-juj71nc5 163 16 , , , cord-274424-juj71nc5 163 17 assigned assign VBN cord-274424-juj71nc5 163 18 gpl90 gpl90 . cord-274424-juj71nc5 163 19 and and CC cord-274424-juj71nc5 163 20 gp2 gp2 NNP cord-274424-juj71nc5 163 21 10 10 CD cord-274424-juj71nc5 163 22 , , , cord-274424-juj71nc5 163 23 while while IN cord-274424-juj71nc5 163 24 the the DT cord-274424-juj71nc5 163 25 SA SA NNP cord-274424-juj71nc5 163 26 protein protein NN cord-274424-juj71nc5 163 27 was be VBD cord-274424-juj71nc5 163 28 expressed express VBN cord-274424-juj71nc5 163 29 in in IN cord-274424-juj71nc5 163 30 human human JJ cord-274424-juj71nc5 163 31 cells cell NNS cord-274424-juj71nc5 163 32 as as IN cord-274424-juj71nc5 163 33 a a DT cord-274424-juj71nc5 163 34 single single JJ cord-274424-juj71nc5 163 35 glycoprotein glycoprotein NN cord-274424-juj71nc5 163 36 species species NN cord-274424-juj71nc5 163 37 designated designate VBN cord-274424-juj71nc5 163 38 gp170 gp170 NNP cord-274424-juj71nc5 163 39 . . . cord-274424-juj71nc5 164 1 The the DT cord-274424-juj71nc5 164 2 gp210 gp210 NNP cord-274424-juj71nc5 164 3 was be VBD cord-274424-juj71nc5 164 4 partially partially RB cord-274424-juj71nc5 164 5 resistant resistant JJ cord-274424-juj71nc5 164 6 to to IN cord-274424-juj71nc5 164 7 Endo Endo NNP cord-274424-juj71nc5 164 8 H H NNP cord-274424-juj71nc5 164 9 , , , cord-274424-juj71nc5 164 10 suggesting suggest VBG cord-274424-juj71nc5 164 11 that that IN cord-274424-juj71nc5 164 12 gpl90 gpl90 NNP cord-274424-juj71nc5 164 13 was be VBD cord-274424-juj71nc5 164 14 modified modify VBN cord-274424-juj71nc5 164 15 to to IN cord-274424-juj71nc5 164 16 gp210 gp210 NNP cord-274424-juj71nc5 164 17 by by IN cord-274424-juj71nc5 164 18 the the DT cord-274424-juj71nc5 164 19 addition addition NN cord-274424-juj71nc5 164 20 of of IN cord-274424-juj71nc5 164 21 complex complex JJ cord-274424-juj71nc5 164 22 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 164 23 at at IN cord-274424-juj71nc5 164 24 the the DT cord-274424-juj71nc5 164 25 Golgi Golgi NNP cord-274424-juj71nc5 164 26 . . . cord-274424-juj71nc5 165 1 The the DT cord-274424-juj71nc5 165 2 SA SA NNP cord-274424-juj71nc5 165 3 protein protein NN cord-274424-juj71nc5 165 4 remained remain VBD cord-274424-juj71nc5 165 5 Endo Endo NNP cord-274424-juj71nc5 165 6 H H NNP cord-274424-juj71nc5 165 7 sensitive sensitive JJ cord-274424-juj71nc5 165 8 even even RB cord-274424-juj71nc5 165 9 after after IN cord-274424-juj71nc5 165 10 extensive extensive JJ cord-274424-juj71nc5 165 11 chasing chasing NN cord-274424-juj71nc5 165 12 , , , cord-274424-juj71nc5 165 13 implying imply VBG cord-274424-juj71nc5 165 14 that that IN cord-274424-juj71nc5 165 15 it -PRON- PRP cord-274424-juj71nc5 165 16 was be VBD cord-274424-juj71nc5 165 17 not not RB cord-274424-juj71nc5 165 18 transported transport VBN cord-274424-juj71nc5 165 19 to to IN cord-274424-juj71nc5 165 20 the the DT cord-274424-juj71nc5 165 21 Golgi Golgi NNP cord-274424-juj71nc5 165 22 stack stack NN cord-274424-juj71nc5 165 23 . . . cord-274424-juj71nc5 166 1 Pulse pulse NN cord-274424-juj71nc5 166 2 - - HYPH cord-274424-juj71nc5 166 3 chase chase NN cord-274424-juj71nc5 166 4 analysis analysis NN cord-274424-juj71nc5 166 5 of of IN cord-274424-juj71nc5 166 6 TGEV TGEV NNP cord-274424-juj71nc5 166 7 - - , cord-274424-juj71nc5 166 8 or or CC cord-274424-juj71nc5 166 9 vTS-1 vTS-1 NNP cord-274424-juj71nc5 167 1 -infected -infected JJ cord-274424-juj71nc5 167 2 cells cell NNS cord-274424-juj71nc5 167 3 demonstrated demonstrate VBD cord-274424-juj71nc5 167 4 that that IN cord-274424-juj71nc5 167 5 S S NNP cord-274424-juj71nc5 167 6 protein protein NN cord-274424-juj71nc5 167 7 was be VBD cord-274424-juj71nc5 167 8 initially initially RB cord-274424-juj71nc5 167 9 synthesized synthesize VBN cord-274424-juj71nc5 167 10 as as IN cord-274424-juj71nc5 167 11 gpl90 gpl90 CD cord-274424-juj71nc5 167 12 but but CC cord-274424-juj71nc5 167 13 gradually gradually RB cord-274424-juj71nc5 167 14 accumulated accumulate VBN cord-274424-juj71nc5 167 15 as as IN cord-274424-juj71nc5 167 16 gp210 gp210 NNP cord-274424-juj71nc5 167 17 due due JJ cord-274424-juj71nc5 167 18 to to IN cord-274424-juj71nc5 167 19 post post JJ cord-274424-juj71nc5 167 20 - - JJ cord-274424-juj71nc5 167 21 translational translational JJ cord-274424-juj71nc5 167 22 modifications modification NNS cord-274424-juj71nc5 167 23 . . . cord-274424-juj71nc5 168 1 Endo endo NN cord-274424-juj71nc5 168 2 H H NNP cord-274424-juj71nc5 168 3 digestion digestion NN cord-274424-juj71nc5 168 4 of of IN cord-274424-juj71nc5 168 5 recombinant recombinant JJ cord-274424-juj71nc5 168 6 S S NNP cord-274424-juj71nc5 168 7 protein protein NN cord-274424-juj71nc5 168 8 expressed express VBN cord-274424-juj71nc5 168 9 in in IN cord-274424-juj71nc5 168 10 HTK HTK NNP cord-274424-juj71nc5 168 11 - - HYPH cord-274424-juj71nc5 168 12 produced produce VBD cord-274424-juj71nc5 168 13 a a DT cord-274424-juj71nc5 168 14 range range NN cord-274424-juj71nc5 168 15 ( ( -LRB- cord-274424-juj71nc5 168 16 n n NN cord-274424-juj71nc5 168 17 / / SYM cord-274424-juj71nc5 168 18 l l NN cord-274424-juj71nc5 168 19 , , , cord-274424-juj71nc5 168 20 160 160 CD cord-274424-juj71nc5 168 21 - - SYM cord-274424-juj71nc5 168 22 210,000 210,000 CD cord-274424-juj71nc5 168 23 ) ) -RRB- cord-274424-juj71nc5 168 24 of of IN cord-274424-juj71nc5 168 25 partially partially RB cord-274424-juj71nc5 168 26 resistant resistant JJ cord-274424-juj71nc5 168 27 polypeptides polypeptide NNS cord-274424-juj71nc5 168 28 . . . cord-274424-juj71nc5 169 1 Both both DT cord-274424-juj71nc5 169 2 of of IN cord-274424-juj71nc5 169 3 these these DT cord-274424-juj71nc5 169 4 observations observation NNS cord-274424-juj71nc5 169 5 were be VBD cord-274424-juj71nc5 169 6 made make VBN cord-274424-juj71nc5 169 7 for for IN cord-274424-juj71nc5 169 8 the the DT cord-274424-juj71nc5 169 9 FIPV FIPV NNS cord-274424-juj71nc5 169 10 ( ( -LRB- cord-274424-juj71nc5 169 11 79-l 79-l CD cord-274424-juj71nc5 169 12 146 146 CD cord-274424-juj71nc5 169 13 ) ) -RRB- cord-274424-juj71nc5 170 1 S S NNP cord-274424-juj71nc5 170 2 protein protein NN cord-274424-juj71nc5 170 3 expressed express VBD cord-274424-juj71nc5 170 4 in in IN cord-274424-juj71nc5 170 5 a a DT cord-274424-juj71nc5 170 6 bovine bovine JJ cord-274424-juj71nc5 170 7 papilloma papilloma NN cord-274424-juj71nc5 170 8 virus virus NN cord-274424-juj71nc5 170 9 transformed transform VBN cord-274424-juj71nc5 170 10 mouse mouse NN cord-274424-juj71nc5 170 11 cell cell NN cord-274424-juj71nc5 170 12 line line NN cord-274424-juj71nc5 170 13 ( ( -LRB- cord-274424-juj71nc5 170 14 de de FW cord-274424-juj71nc5 170 15 Groot Groot NNP cord-274424-juj71nc5 170 16 et et NNP cord-274424-juj71nc5 170 17 a/. a/. NN cord-274424-juj71nc5 170 18 , , , cord-274424-juj71nc5 170 19 1989 1989 CD cord-274424-juj71nc5 170 20 ) ) -RRB- cord-274424-juj71nc5 170 21 . . . cord-274424-juj71nc5 171 1 However however RB cord-274424-juj71nc5 171 2 , , , cord-274424-juj71nc5 171 3 the the DT cord-274424-juj71nc5 171 4 TGEV TGEV NNP cord-274424-juj71nc5 171 5 S S NNP cord-274424-juj71nc5 171 6 gene gene NN cord-274424-juj71nc5 171 7 expressed express VBN cord-274424-juj71nc5 171 8 by by IN cord-274424-juj71nc5 171 9 a a DT cord-274424-juj71nc5 171 10 rVV rvv NN cord-274424-juj71nc5 171 11 in in IN cord-274424-juj71nc5 171 12 porcine porcine JJ cord-274424-juj71nc5 171 13 LLC LLC NNP cord-274424-juj71nc5 171 14 - - HYPH cord-274424-juj71nc5 171 15 PKl PKl NNP cord-274424-juj71nc5 171 16 cells cell NNS cord-274424-juj71nc5 171 17 produced produce VBD cord-274424-juj71nc5 171 18 an an DT cord-274424-juj71nc5 171 19 Endo Endo NNP cord-274424-juj71nc5 171 20 H h NN cord-274424-juj71nc5 171 21 - - HYPH cord-274424-juj71nc5 171 22 resistant resistant JJ cord-274424-juj71nc5 171 23 gp210 gp210 NNP cord-274424-juj71nc5 171 24 product product NN cord-274424-juj71nc5 171 25 with with IN cord-274424-juj71nc5 171 26 no no DT cord-274424-juj71nc5 171 27 partially partially RB cord-274424-juj71nc5 171 28 resistant resistant JJ cord-274424-juj71nc5 171 29 Endo endo NN cord-274424-juj71nc5 171 30 H H NNP cord-274424-juj71nc5 171 31 intermediates intermediate NNS cord-274424-juj71nc5 171 32 . . . cord-274424-juj71nc5 172 1 A a DT cord-274424-juj71nc5 172 2 similar similar JJ cord-274424-juj71nc5 172 3 observation observation NN cord-274424-juj71nc5 172 4 was be VBD cord-274424-juj71nc5 172 5 also also RB cord-274424-juj71nc5 172 6 made make VBN cord-274424-juj71nc5 172 7 for for IN cord-274424-juj71nc5 172 8 the the DT cord-274424-juj71nc5 172 9 FIPV FIPV NNP cord-274424-juj71nc5 172 10 S S NNP cord-274424-juj71nc5 172 11 gene gene NN cord-274424-juj71nc5 172 12 expressed express VBN cord-274424-juj71nc5 172 13 by by IN cord-274424-juj71nc5 172 14 a a DT cord-274424-juj71nc5 172 15 rW rw NN cord-274424-juj71nc5 172 16 in in IN cord-274424-juj71nc5 172 17 feline feline JJ cord-274424-juj71nc5 172 18 cells cell NNS cord-274424-juj71nc5 172 19 ( ( -LRB- cord-274424-juj71nc5 172 20 Vennema Vennema NNP cord-274424-juj71nc5 172 21 et et FW cord-274424-juj71nc5 172 22 al al NNP cord-274424-juj71nc5 172 23 . . NNP cord-274424-juj71nc5 172 24 , , , cord-274424-juj71nc5 172 25 1990 1990 CD cord-274424-juj71nc5 172 26 ) ) -RRB- cord-274424-juj71nc5 172 27 , , , cord-274424-juj71nc5 172 28 suggesting suggest VBG cord-274424-juj71nc5 172 29 that that IN cord-274424-juj71nc5 172 30 expression expression NN cord-274424-juj71nc5 172 31 of of IN cord-274424-juj71nc5 172 32 coronavirus coronavirus NN cord-274424-juj71nc5 172 33 S s NN cord-274424-juj71nc5 172 34 proteins protein NNS cord-274424-juj71nc5 172 35 in in IN cord-274424-juj71nc5 172 36 a a DT cord-274424-juj71nc5 172 37 cell cell NN cord-274424-juj71nc5 172 38 line line NN cord-274424-juj71nc5 172 39 compatible compatible JJ cord-274424-juj71nc5 172 40 with with IN cord-274424-juj71nc5 172 41 their -PRON- PRP$ cord-274424-juj71nc5 172 42 origin origin NN cord-274424-juj71nc5 172 43 may may MD cord-274424-juj71nc5 172 44 have have VB cord-274424-juj71nc5 172 45 profound profound JJ cord-274424-juj71nc5 172 46 effects effect NNS cord-274424-juj71nc5 172 47 upon upon IN cord-274424-juj71nc5 172 48 the the DT cord-274424-juj71nc5 172 49 extent extent NN cord-274424-juj71nc5 172 50 of of IN cord-274424-juj71nc5 172 51 post post JJ cord-274424-juj71nc5 172 52 - - JJ cord-274424-juj71nc5 172 53 translational translational JJ cord-274424-juj71nc5 172 54 processing processing NN cord-274424-juj71nc5 172 55 of of IN cord-274424-juj71nc5 172 56 these these DT cord-274424-juj71nc5 172 57 proteins protein NNS cord-274424-juj71nc5 172 58 . . . cord-274424-juj71nc5 173 1 The the DT cord-274424-juj71nc5 173 2 acquisition acquisition NN cord-274424-juj71nc5 173 3 of of IN cord-274424-juj71nc5 173 4 Endo Endo NNP cord-274424-juj71nc5 173 5 H H NNP cord-274424-juj71nc5 173 6 resistance resistance NN cord-274424-juj71nc5 173 7 was be VBD cord-274424-juj71nc5 173 8 significantly significantly RB cord-274424-juj71nc5 173 9 retarded retarded JJ cord-274424-juj71nc5 173 10 for for IN cord-274424-juj71nc5 173 11 recombinant recombinant JJ cord-274424-juj71nc5 173 12 S S NNP cord-274424-juj71nc5 173 13 protein protein NN cord-274424-juj71nc5 173 14 compared compare VBN cord-274424-juj71nc5 173 15 to to IN cord-274424-juj71nc5 173 16 S S NNP cord-274424-juj71nc5 173 17 protein protein NN cord-274424-juj71nc5 173 18 expressed express VBD cord-274424-juj71nc5 173 19 during during IN cord-274424-juj71nc5 173 20 a a DT cord-274424-juj71nc5 173 21 TGEV TGEV NNP cord-274424-juj71nc5 173 22 infection infection NN cord-274424-juj71nc5 173 23 . . . cord-274424-juj71nc5 174 1 Retardation retardation NN cord-274424-juj71nc5 174 2 of of IN cord-274424-juj71nc5 174 3 coronavirus coronavirus NN cord-274424-juj71nc5 174 4 S s NN cord-274424-juj71nc5 174 5 protein protein NN cord-274424-juj71nc5 174 6 intracellular intracellular JJ cord-274424-juj71nc5 174 7 transport transport NN cord-274424-juj71nc5 174 8 has have VBZ cord-274424-juj71nc5 174 9 also also RB cord-274424-juj71nc5 174 10 been be VBN cord-274424-juj71nc5 174 11 observed observe VBN cord-274424-juj71nc5 174 12 for for IN cord-274424-juj71nc5 174 13 the the DT cord-274424-juj71nc5 174 14 FIPV FIPV NNPS cord-274424-juj71nc5 174 15 and and CC cord-274424-juj71nc5 174 16 MHV MHV NNP cord-274424-juj71nc5 174 17 S S NNP cord-274424-juj71nc5 174 18 proteins protein NNS cord-274424-juj71nc5 174 19 expressed express VBN cord-274424-juj71nc5 174 20 by by IN cord-274424-juj71nc5 174 21 rVVs rvvs XX cord-274424-juj71nc5 175 1 ( ( -LRB- cord-274424-juj71nc5 175 2 Vennema Vennema NNP cord-274424-juj71nc5 175 3 et et FW cord-274424-juj71nc5 175 4 al al NNP cord-274424-juj71nc5 175 5 . . NNP cord-274424-juj71nc5 175 6 , , , cord-274424-juj71nc5 175 7 1990 1990 CD cord-274424-juj71nc5 175 8 ) ) -RRB- cord-274424-juj71nc5 175 9 . . . cord-274424-juj71nc5 176 1 In in IN cord-274424-juj71nc5 176 2 this this DT cord-274424-juj71nc5 176 3 study study NN cord-274424-juj71nc5 176 4 , , , cord-274424-juj71nc5 176 5 retardation retardation NN cord-274424-juj71nc5 176 6 between between IN cord-274424-juj71nc5 176 7 the the DT cord-274424-juj71nc5 176 8 RER rer NN cord-274424-juj71nc5 176 9 and and CC cord-274424-juj71nc5 176 10 the the DT cord-274424-juj71nc5 176 11 Golgi Golgi NNP cord-274424-juj71nc5 176 12 in in IN cord-274424-juj71nc5 176 13 the the DT cord-274424-juj71nc5 176 14 absence absence NN cord-274424-juj71nc5 176 15 of of IN cord-274424-juj71nc5 176 16 virus virus NN cord-274424-juj71nc5 176 17 budding budding NN cord-274424-juj71nc5 176 18 was be VBD cord-274424-juj71nc5 176 19 interpreted interpret VBN cord-274424-juj71nc5 176 20 as as IN cord-274424-juj71nc5 176 21 a a DT cord-274424-juj71nc5 176 22 transient transient JJ cord-274424-juj71nc5 176 23 accumulation accumulation NN cord-274424-juj71nc5 176 24 of of IN cord-274424-juj71nc5 176 25 the the DT cord-274424-juj71nc5 176 26 S S NNP cord-274424-juj71nc5 176 27 protein protein NN cord-274424-juj71nc5 176 28 at at IN cord-274424-juj71nc5 176 29 or or CC cord-274424-juj71nc5 176 30 near near IN cord-274424-juj71nc5 176 31 the the DT cord-274424-juj71nc5 176 32 site site NN cord-274424-juj71nc5 176 33 of of IN cord-274424-juj71nc5 176 34 virus virus NN cord-274424-juj71nc5 176 35 budding bud VBG cord-274424-juj71nc5 176 36 and and CC cord-274424-juj71nc5 176 37 suggested suggest VBD cord-274424-juj71nc5 176 38 that that IN cord-274424-juj71nc5 176 39 the the DT cord-274424-juj71nc5 176 40 S S NNP cord-274424-juj71nc5 176 41 protein protein NN cord-274424-juj71nc5 176 42 may may MD cord-274424-juj71nc5 176 43 have have VB cord-274424-juj71nc5 176 44 a a DT cord-274424-juj71nc5 176 45 role role NN cord-274424-juj71nc5 176 46 in in IN cord-274424-juj71nc5 176 47 defining define VBG cord-274424-juj71nc5 176 48 the the DT cord-274424-juj71nc5 176 49 site site NN cord-274424-juj71nc5 176 50 of of IN cord-274424-juj71nc5 176 51 virus virus NN cord-274424-juj71nc5 176 52 budding bud VBG cord-274424-juj71nc5 176 53 , , , cord-274424-juj71nc5 176 54 a a DT cord-274424-juj71nc5 176 55 func func NN cord-274424-juj71nc5 176 56 - - HYPH cord-274424-juj71nc5 176 57 tion tion NN cord-274424-juj71nc5 176 58 previously previously RB cord-274424-juj71nc5 176 59 ascribed ascribe VBN cord-274424-juj71nc5 176 60 to to IN cord-274424-juj71nc5 176 61 the the DT cord-274424-juj71nc5 176 62 M M NNP cord-274424-juj71nc5 176 63 proteins protein NNS cord-274424-juj71nc5 176 64 of of IN cord-274424-juj71nc5 176 65 coronaviruses coronaviruse NNS cord-274424-juj71nc5 177 1 ( ( -LRB- cord-274424-juj71nc5 177 2 Booze booze NN cord-274424-juj71nc5 177 3 et et NNP cord-274424-juj71nc5 177 4 al al NNP cord-274424-juj71nc5 177 5 . . NNP cord-274424-juj71nc5 177 6 , , , cord-274424-juj71nc5 177 7 1985 1985 CD cord-274424-juj71nc5 177 8 ) ) -RRB- cord-274424-juj71nc5 177 9 . . . cord-274424-juj71nc5 178 1 lmmunofluorescence lmmunofluorescence NNP cord-274424-juj71nc5 178 2 studies study VBZ cord-274424-juj71nc5 178 3 with with IN cord-274424-juj71nc5 178 4 vTS vTS NNP cord-274424-juj71nc5 178 5 - - HYPH cord-274424-juj71nc5 178 6 infected infect VBN cord-274424-juj71nc5 178 7 cells cell NNS cord-274424-juj71nc5 178 8 demonstrated demonstrate VBD cord-274424-juj71nc5 178 9 that that IN cord-274424-juj71nc5 178 10 recombinant recombinant JJ cord-274424-juj71nc5 178 11 S S NNP cord-274424-juj71nc5 178 12 protein protein NN cord-274424-juj71nc5 178 13 accumulated accumulate VBN cord-274424-juj71nc5 178 14 in in IN cord-274424-juj71nc5 178 15 a a DT cord-274424-juj71nc5 178 16 polar polar JJ cord-274424-juj71nc5 178 17 perinuclear perinuclear NNP cord-274424-juj71nc5 178 18 compartment compartment NN cord-274424-juj71nc5 178 19 of of IN cord-274424-juj71nc5 178 20 the the DT cord-274424-juj71nc5 178 21 cell cell NN cord-274424-juj71nc5 178 22 , , , cord-274424-juj71nc5 178 23 a a DT cord-274424-juj71nc5 178 24 similar similar JJ cord-274424-juj71nc5 178 25 observation observation NN cord-274424-juj71nc5 178 26 was be VBD cord-274424-juj71nc5 178 27 seen see VBN cord-274424-juj71nc5 178 28 for for IN cord-274424-juj71nc5 178 29 the the DT cord-274424-juj71nc5 178 30 cellular cellular JJ cord-274424-juj71nc5 178 31 localization localization NN cord-274424-juj71nc5 178 32 of of IN cord-274424-juj71nc5 178 33 rVV rVV NNP cord-274424-juj71nc5 178 34 expressed express VBD cord-274424-juj71nc5 178 35 TGEV TGEV NNP cord-274424-juj71nc5 178 36 M M NNP cord-274424-juj71nc5 178 37 protein protein NN cord-274424-juj71nc5 178 38 ( ( -LRB- cord-274424-juj71nc5 178 39 Pulford Pulford NNP cord-274424-juj71nc5 178 40 and and CC cord-274424-juj71nc5 178 41 Britton Britton NNP cord-274424-juj71nc5 178 42 , , , cord-274424-juj71nc5 178 43 1990 1990 CD cord-274424-juj71nc5 178 44 ) ) -RRB- cord-274424-juj71nc5 178 45 and and CC cord-274424-juj71nc5 178 46 at at IN cord-274424-juj71nc5 178 47 the the DT cord-274424-juj71nc5 178 48 cell cell NN cord-274424-juj71nc5 178 49 surface surface NN cord-274424-juj71nc5 178 50 . . . cord-274424-juj71nc5 179 1 The the DT cord-274424-juj71nc5 179 2 presence presence NN cord-274424-juj71nc5 179 3 of of IN cord-274424-juj71nc5 179 4 TGEV TGEV NNP cord-274424-juj71nc5 179 5 gp210 gp210 NNP cord-274424-juj71nc5 179 6 in in IN cord-274424-juj71nc5 179 7 the the DT cord-274424-juj71nc5 179 8 culture culture NN cord-274424-juj71nc5 179 9 medium medium NN cord-274424-juj71nc5 179 10 of of IN cord-274424-juj71nc5 179 11 vTS vts NN cord-274424-juj71nc5 179 12 - - HYPH cord-274424-juj71nc5 179 13 infected infect VBN cord-274424-juj71nc5 179 14 cells cell NNS cord-274424-juj71nc5 179 15 suggested suggest VBD cord-274424-juj71nc5 179 16 that that IN cord-274424-juj71nc5 179 17 gp210 gp210 NNP cord-274424-juj71nc5 179 18 may may MD cord-274424-juj71nc5 179 19 be be VB cord-274424-juj71nc5 179 20 released release VBN cord-274424-juj71nc5 179 21 from from IN cord-274424-juj71nc5 179 22 the the DT cord-274424-juj71nc5 179 23 cell cell NN cord-274424-juj71nc5 179 24 . . . cord-274424-juj71nc5 180 1 Proteins protein NNS cord-274424-juj71nc5 180 2 can can MD cord-274424-juj71nc5 180 3 be be VB cord-274424-juj71nc5 180 4 transported transport VBN cord-274424-juj71nc5 180 5 from from IN cord-274424-juj71nc5 180 6 the the DT cord-274424-juj71nc5 180 7 RER RER NNP cord-274424-juj71nc5 180 8 to to IN cord-274424-juj71nc5 180 9 the the DT cord-274424-juj71nc5 180 10 Golgi Golgi NNP cord-274424-juj71nc5 180 11 by by IN cord-274424-juj71nc5 180 12 interacting interact VBG cord-274424-juj71nc5 180 13 with with IN cord-274424-juj71nc5 180 14 carrier carrier NN cord-274424-juj71nc5 180 15 proteins protein NNS cord-274424-juj71nc5 180 16 or or CC cord-274424-juj71nc5 180 17 following follow VBG cord-274424-juj71nc5 180 18 membrane membrane NN cord-274424-juj71nc5 180 19 incorporation incorporation NN cord-274424-juj71nc5 180 20 , , , cord-274424-juj71nc5 180 21 by by IN cord-274424-juj71nc5 180 22 the the DT cord-274424-juj71nc5 180 23 flow flow NN cord-274424-juj71nc5 180 24 of of IN cord-274424-juj71nc5 180 25 membrane membrane NN cord-274424-juj71nc5 180 26 from from IN cord-274424-juj71nc5 180 27 the the DT cord-274424-juj71nc5 180 28 ER ER NNP cord-274424-juj71nc5 180 29 to to IN cord-274424-juj71nc5 180 30 the the DT cord-274424-juj71nc5 180 31 cell cell NN cord-274424-juj71nc5 180 32 surface surface NN cord-274424-juj71nc5 180 33 . . . cord-274424-juj71nc5 181 1 This this DT cord-274424-juj71nc5 181 2 membrane membrane NN cord-274424-juj71nc5 181 3 flow flow NN cord-274424-juj71nc5 181 4 may may MD cord-274424-juj71nc5 181 5 be be VB cord-274424-juj71nc5 181 6 responsible responsible JJ cord-274424-juj71nc5 181 7 for for IN cord-274424-juj71nc5 181 8 the the DT cord-274424-juj71nc5 181 9 cell cell NN cord-274424-juj71nc5 181 10 surface surface NN cord-274424-juj71nc5 181 11 presence presence NN cord-274424-juj71nc5 181 12 of of IN cord-274424-juj71nc5 181 13 coronavirus coronavirus NN cord-274424-juj71nc5 181 14 S s NN cord-274424-juj71nc5 181 15 proteins protein NNS cord-274424-juj71nc5 181 16 . . . cord-274424-juj71nc5 182 1 The the DT cord-274424-juj71nc5 182 2 SA SA NNP cord-274424-juj71nc5 182 3 protein protein NN cord-274424-juj71nc5 182 4 was be VBD cord-274424-juj71nc5 182 5 not not RB cord-274424-juj71nc5 182 6 processed process VBN cord-274424-juj71nc5 182 7 in in IN cord-274424-juj71nc5 182 8 the the DT cord-274424-juj71nc5 182 9 Golgi Golgi NNP cord-274424-juj71nc5 182 10 apparatus apparatus NN cord-274424-juj71nc5 182 11 , , , cord-274424-juj71nc5 182 12 nor nor CC cord-274424-juj71nc5 182 13 found find VBN cord-274424-juj71nc5 182 14 on on IN cord-274424-juj71nc5 182 15 the the DT cord-274424-juj71nc5 182 16 cell cell NN cord-274424-juj71nc5 182 17 surface surface NN cord-274424-juj71nc5 182 18 nor nor CC cord-274424-juj71nc5 182 19 found find VBN cord-274424-juj71nc5 182 20 to to TO cord-274424-juj71nc5 182 21 be be VB cord-274424-juj71nc5 182 22 exported export VBN cord-274424-juj71nc5 182 23 into into IN cord-274424-juj71nc5 182 24 the the DT cord-274424-juj71nc5 182 25 extracellular extracellular JJ cord-274424-juj71nc5 182 26 medium medium NN cord-274424-juj71nc5 182 27 , , , cord-274424-juj71nc5 182 28 indicating indicate VBG cord-274424-juj71nc5 182 29 that that IN cord-274424-juj71nc5 182 30 the the DT cord-274424-juj71nc5 182 31 C C NNP cord-274424-juj71nc5 182 32 - - HYPH cord-274424-juj71nc5 182 33 terminal terminal NN cord-274424-juj71nc5 182 34 membrane membrane NN cord-274424-juj71nc5 182 35 anchor anchor NN cord-274424-juj71nc5 182 36 domain domain NN cord-274424-juj71nc5 182 37 of of IN cord-274424-juj71nc5 182 38 TGEV TGEV NNP cord-274424-juj71nc5 182 39 S S NNP cord-274424-juj71nc5 182 40 protein protein NN cord-274424-juj71nc5 182 41 may may MD cord-274424-juj71nc5 182 42 be be VB cord-274424-juj71nc5 182 43 required require VBN cord-274424-juj71nc5 182 44 for for IN cord-274424-juj71nc5 182 45 all all DT cord-274424-juj71nc5 182 46 of of IN cord-274424-juj71nc5 182 47 these these DT cord-274424-juj71nc5 182 48 functions function NNS cord-274424-juj71nc5 182 49 . . . cord-274424-juj71nc5 183 1 Puddington Puddington NNP cord-274424-juj71nc5 183 2 eta/. eta/. NN cord-274424-juj71nc5 183 3 ( ( -LRB- cord-274424-juj71nc5 183 4 1986 1986 CD cord-274424-juj71nc5 183 5 ) ) -RRB- cord-274424-juj71nc5 183 6 used use VBD cord-274424-juj71nc5 183 7 a a DT cord-274424-juj71nc5 183 8 mutant mutant JJ cord-274424-juj71nc5 183 9 VSV VSV NNP cord-274424-juj71nc5 183 10 G G NNP cord-274424-juj71nc5 183 11 protein protein NN cord-274424-juj71nc5 183 12 to to TO cord-274424-juj71nc5 183 13 establish establish VB cord-274424-juj71nc5 183 14 that that IN cord-274424-juj71nc5 183 15 the the DT cord-274424-juj71nc5 183 16 cytoplasmic cytoplasmic JJ cord-274424-juj71nc5 183 17 tail tail NN cord-274424-juj71nc5 183 18 of of IN cord-274424-juj71nc5 183 19 the the DT cord-274424-juj71nc5 183 20 G g NN cord-274424-juj71nc5 183 21 protein protein NN cord-274424-juj71nc5 183 22 was be VBD cord-274424-juj71nc5 183 23 essential essential JJ cord-274424-juj71nc5 183 24 for for IN cord-274424-juj71nc5 183 25 its -PRON- PRP$ cord-274424-juj71nc5 183 26 transport transport NN cord-274424-juj71nc5 183 27 through through IN cord-274424-juj71nc5 183 28 the the DT cord-274424-juj71nc5 183 29 Golgi Golgi NNP cord-274424-juj71nc5 183 30 stack stack NN cord-274424-juj71nc5 183 31 . . . cord-274424-juj71nc5 184 1 In in IN cord-274424-juj71nc5 184 2 addition addition NN cord-274424-juj71nc5 184 3 , , , cord-274424-juj71nc5 184 4 Bray Bray NNP cord-274424-juj71nc5 184 5 eta/. eta/. CD cord-274424-juj71nc5 184 6 ( ( -LRB- cord-274424-juj71nc5 184 7 1989 1989 CD cord-274424-juj71nc5 184 8 ) ) -RRB- cord-274424-juj71nc5 184 9 used use VBD cord-274424-juj71nc5 184 10 rWs rws NN cord-274424-juj71nc5 184 11 to to TO cord-274424-juj71nc5 184 12 demonstrate demonstrate VB cord-274424-juj71nc5 184 13 that that IN cord-274424-juj71nc5 184 14 a a DT cord-274424-juj71nc5 184 15 Dengue Dengue NNP cord-274424-juj71nc5 184 16 virus virus NN cord-274424-juj71nc5 184 17 envelope envelope NN cord-274424-juj71nc5 184 18 protein protein NN cord-274424-juj71nc5 184 19 truncated truncate VBD cord-274424-juj71nc5 184 20 at at IN cord-274424-juj71nc5 184 21 the the DT cord-274424-juj71nc5 184 22 C C NNP cord-274424-juj71nc5 184 23 - - HYPH cord-274424-juj71nc5 184 24 terminus terminus NN cord-274424-juj71nc5 184 25 , , , cord-274424-juj71nc5 184 26 unlike unlike IN cord-274424-juj71nc5 184 27 the the DT cord-274424-juj71nc5 184 28 wild wild JJ cord-274424-juj71nc5 184 29 - - HYPH cord-274424-juj71nc5 184 30 type type NN cord-274424-juj71nc5 184 31 protein protein NN cord-274424-juj71nc5 184 32 , , , cord-274424-juj71nc5 184 33 was be VBD cord-274424-juj71nc5 184 34 not not RB cord-274424-juj71nc5 184 35 secreted secrete VBN cord-274424-juj71nc5 184 36 into into IN cord-274424-juj71nc5 184 37 the the DT cord-274424-juj71nc5 184 38 extracellular extracellular JJ cord-274424-juj71nc5 184 39 medium medium NN cord-274424-juj71nc5 184 40 . . . cord-274424-juj71nc5 185 1 However however RB cord-274424-juj71nc5 185 2 , , , cord-274424-juj71nc5 185 3 Sveda Sveda NNP cord-274424-juj71nc5 185 4 et et NNP cord-274424-juj71nc5 185 5 a/. a/. NN cord-274424-juj71nc5 185 6 ( ( -LRB- cord-274424-juj71nc5 185 7 1982 1982 CD cord-274424-juj71nc5 185 8 ) ) -RRB- cord-274424-juj71nc5 185 9 found find VBD cord-274424-juj71nc5 185 10 that that IN cord-274424-juj71nc5 185 11 C c NN cord-274424-juj71nc5 185 12 - - HYPH cord-274424-juj71nc5 185 13 terminal terminal NN cord-274424-juj71nc5 185 14 sequences sequence NNS cord-274424-juj71nc5 185 15 of of IN cord-274424-juj71nc5 185 16 the the DT cord-274424-juj71nc5 185 17 influenza influenza NN cord-274424-juj71nc5 185 18 virus virus NN cord-274424-juj71nc5 185 19 hemagglutinin hemagglutinin NNP cord-274424-juj71nc5 185 20 ( ( -LRB- cord-274424-juj71nc5 185 21 HA HA NNP cord-274424-juj71nc5 185 22 ) ) -RRB- cord-274424-juj71nc5 185 23 were be VBD cord-274424-juj71nc5 185 24 essential essential JJ cord-274424-juj71nc5 185 25 for for IN cord-274424-juj71nc5 185 26 cell cell NN cord-274424-juj71nc5 185 27 surface surface NN cord-274424-juj71nc5 185 28 expression expression NN cord-274424-juj71nc5 185 29 and and CC cord-274424-juj71nc5 185 30 that that IN cord-274424-juj71nc5 185 31 mutants mutant NNS cord-274424-juj71nc5 185 32 with with IN cord-274424-juj71nc5 185 33 a a DT cord-274424-juj71nc5 185 34 disrupted disrupt VBN cord-274424-juj71nc5 185 35 anchor anchor NN cord-274424-juj71nc5 185 36 domain domain NN cord-274424-juj71nc5 185 37 were be VBD cord-274424-juj71nc5 185 38 secreted secrete VBN cord-274424-juj71nc5 185 39 , , , cord-274424-juj71nc5 185 40 suggesting suggest VBG cord-274424-juj71nc5 185 41 that that IN cord-274424-juj71nc5 185 42 influenza influenza NN cord-274424-juj71nc5 185 43 virus virus NN cord-274424-juj71nc5 185 44 HA HA NNP cord-274424-juj71nc5 185 45 , , , cord-274424-juj71nc5 185 46 unlike unlike IN cord-274424-juj71nc5 185 47 TGEV TGEV NNP cord-274424-juj71nc5 185 48 , , , cord-274424-juj71nc5 185 49 VSV VSV NNP cord-274424-juj71nc5 185 50 , , , cord-274424-juj71nc5 185 51 and and CC cord-274424-juj71nc5 185 52 Dengue Dengue NNP cord-274424-juj71nc5 185 53 virus virus NN cord-274424-juj71nc5 185 54 envelope envelope NN cord-274424-juj71nc5 185 55 proteins protein NNS cord-274424-juj71nc5 185 56 , , , cord-274424-juj71nc5 185 57 underwent undergo VBD cord-274424-juj71nc5 185 58 a a DT cord-274424-juj71nc5 185 59 different different JJ cord-274424-juj71nc5 185 60 protein protein NN cord-274424-juj71nc5 185 61 maturation maturation NN cord-274424-juj71nc5 185 62 pathway pathway NN cord-274424-juj71nc5 185 63 . . . cord-274424-juj71nc5 186 1 Evidence evidence NN cord-274424-juj71nc5 186 2 suggests suggest VBZ cord-274424-juj71nc5 186 3 that that IN cord-274424-juj71nc5 186 4 unless unless IN cord-274424-juj71nc5 186 5 a a DT cord-274424-juj71nc5 186 6 membrane membrane NN cord-274424-juj71nc5 186 7 glycoprotein glycoprotein NN cord-274424-juj71nc5 186 8 folds fold VBZ cord-274424-juj71nc5 186 9 into into IN cord-274424-juj71nc5 186 10 a a DT cord-274424-juj71nc5 186 11 native native JJ cord-274424-juj71nc5 186 12 or or CC cord-274424-juj71nc5 186 13 near near RB cord-274424-juj71nc5 186 14 - - HYPH cord-274424-juj71nc5 186 15 native native JJ cord-274424-juj71nc5 186 16 conformation conformation NN cord-274424-juj71nc5 186 17 it -PRON- PRP cord-274424-juj71nc5 186 18 will will MD cord-274424-juj71nc5 186 19 not not RB cord-274424-juj71nc5 186 20 be be VB cord-274424-juj71nc5 186 21 exported export VBN cord-274424-juj71nc5 186 22 from from IN cord-274424-juj71nc5 186 23 the the DT cord-274424-juj71nc5 186 24 RER RER NNP cord-274424-juj71nc5 186 25 ( ( -LRB- cord-274424-juj71nc5 186 26 Lodish Lodish NNP cord-274424-juj71nc5 186 27 , , , cord-274424-juj71nc5 186 28 1988 1988 CD cord-274424-juj71nc5 186 29 ) ) -RRB- cord-274424-juj71nc5 186 30 . . . cord-274424-juj71nc5 187 1 However however RB cord-274424-juj71nc5 187 2 , , , cord-274424-juj71nc5 187 3 the the DT cord-274424-juj71nc5 187 4 general general JJ cord-274424-juj71nc5 187 5 conformation conformation NN cord-274424-juj71nc5 187 6 and and CC cord-274424-juj71nc5 187 7 antigenicity antigenicity NN cord-274424-juj71nc5 187 8 of of IN cord-274424-juj71nc5 187 9 the the DT cord-274424-juj71nc5 187 10 SA SA NNP cord-274424-juj71nc5 187 11 protein protein NN cord-274424-juj71nc5 187 12 appeared appear VBD cord-274424-juj71nc5 187 13 to to TO cord-274424-juj71nc5 187 14 be be VB cord-274424-juj71nc5 187 15 retained retain VBN cord-274424-juj71nc5 187 16 as as IN cord-274424-juj71nc5 187 17 it -PRON- PRP cord-274424-juj71nc5 187 18 reacted react VBD cord-274424-juj71nc5 187 19 with with IN cord-274424-juj71nc5 187 20 both both DT cord-274424-juj71nc5 187 21 polyclonal polyclonal JJ cord-274424-juj71nc5 187 22 and and CC cord-274424-juj71nc5 187 23 monoclonal monoclonal JJ cord-274424-juj71nc5 187 24 TGEV TGEV NNP cord-274424-juj71nc5 187 25 S S NNP cord-274424-juj71nc5 187 26 antisera antisera NN cord-274424-juj71nc5 187 27 in in IN cord-274424-juj71nc5 187 28 contrast contrast NN cord-274424-juj71nc5 187 29 to to IN cord-274424-juj71nc5 187 30 denatured denatured JJ cord-274424-juj71nc5 187 31 S S NNP cord-274424-juj71nc5 187 32 protein protein NN cord-274424-juj71nc5 187 33 or or CC cord-274424-juj71nc5 187 34 S S NNP cord-274424-juj71nc5 187 35 protein protein NN cord-274424-juj71nc5 187 36 expressed express VBN cord-274424-juj71nc5 187 37 in in IN cord-274424-juj71nc5 187 38 E. E. NNP cord-274424-juj71nc5 188 1 co/ co/ NNP cord-274424-juj71nc5 189 1 i i PRP cord-274424-juj71nc5 189 2 cells cells VBP cord-274424-juj71nc5 190 1 ( ( -LRB- cord-274424-juj71nc5 190 2 Correa Correa NNP cord-274424-juj71nc5 190 3 et et NNP cord-274424-juj71nc5 190 4 a/. a/. NNP cord-274424-juj71nc5 190 5 , , , cord-274424-juj71nc5 190 6 1990 1990 CD cord-274424-juj71nc5 190 7 ; ; : cord-274424-juj71nc5 190 8 Delmas Delmas NNP cord-274424-juj71nc5 190 9 et et NNP cord-274424-juj71nc5 190 10 a/. a/. NNP cord-274424-juj71nc5 190 11 , , , cord-274424-juj71nc5 190 12 1990 1990 CD cord-274424-juj71nc5 190 13 ; ; : cord-274424-juj71nc5 190 14 Pulford Pulford NNP cord-274424-juj71nc5 190 15 , , , cord-274424-juj71nc5 190 16 unpublished unpublished JJ cord-274424-juj71nc5 190 17 observations observation NNS cord-274424-juj71nc5 190 18 ) ) -RRB- cord-274424-juj71nc5 190 19 . . . cord-274424-juj71nc5 191 1 The the DT cord-274424-juj71nc5 191 2 results result NNS cord-274424-juj71nc5 191 3 presented present VBN cord-274424-juj71nc5 191 4 in in IN cord-274424-juj71nc5 191 5 this this DT cord-274424-juj71nc5 191 6 paper paper NN cord-274424-juj71nc5 191 7 suggest suggest VBP cord-274424-juj71nc5 191 8 that that IN cord-274424-juj71nc5 191 9 the the DT cord-274424-juj71nc5 191 10 SA SA NNP cord-274424-juj71nc5 191 11 protein protein NN cord-274424-juj71nc5 191 12 was be VBD cord-274424-juj71nc5 191 13 retained retain VBN cord-274424-juj71nc5 191 14 in in IN cord-274424-juj71nc5 191 15 the the DT cord-274424-juj71nc5 191 16 RER RER NNP cord-274424-juj71nc5 191 17 possibly possibly RB cord-274424-juj71nc5 191 18 due due IN cord-274424-juj71nc5 191 19 to to IN cord-274424-juj71nc5 191 20 the the DT cord-274424-juj71nc5 191 21 removal removal NN cord-274424-juj71nc5 191 22 of of IN cord-274424-juj71nc5 191 23 essential essential JJ cord-274424-juj71nc5 191 24 C c NN cord-274424-juj71nc5 191 25 - - HYPH cord-274424-juj71nc5 191 26 terminal terminal NN cord-274424-juj71nc5 191 27 transport transport NN cord-274424-juj71nc5 191 28 signals signal NNS cord-274424-juj71nc5 191 29 . . . cord-274424-juj71nc5 192 1 We -PRON- PRP cord-274424-juj71nc5 192 2 would would MD cord-274424-juj71nc5 192 3 like like VB cord-274424-juj71nc5 192 4 to to TO cord-274424-juj71nc5 192 5 thank thank VB cord-274424-juj71nc5 192 6 Miss Miss NNP cord-274424-juj71nc5 192 7 K. K. NNP cord-274424-juj71nc5 192 8 Mawditt Mawditt NNP cord-274424-juj71nc5 192 9 for for IN cord-274424-juj71nc5 192 10 synthesizing synthesize VBG cord-274424-juj71nc5 192 11 the the DT cord-274424-juj71nc5 192 12 oligonucleotide oligonucleotide NN cord-274424-juj71nc5 192 13 used use VBN cord-274424-juj71nc5 192 14 in in IN cord-274424-juj71nc5 192 15 the the DT cord-274424-juj71nc5 192 16 sequencing sequencing NN cord-274424-juj71nc5 192 17 , , , cord-274424-juj71nc5 192 18 Mrs. Mrs. NNP cord-274424-juj71nc5 192 19 A. A. NNP cord-274424-juj71nc5 192 20 Waite Waite NNP cord-274424-juj71nc5 192 21 for for IN cord-274424-juj71nc5 192 22 carrying carry VBG cord-274424-juj71nc5 192 23 out out RP cord-274424-juj71nc5 192 24 the the DT cord-274424-juj71nc5 192 25 animal animal NN cord-274424-juj71nc5 192 26 inoculations inoculation NNS cord-274424-juj71nc5 192 27 and and CC cord-274424-juj71nc5 192 28 Dr Dr NNP cord-274424-juj71nc5 192 29 D. D. NNP cord-274424-juj71nc5 192 30 Pocock Pocock NNP cord-274424-juj71nc5 192 31 for for IN cord-274424-juj71nc5 192 32 helpful helpful JJ cord-274424-juj71nc5 192 33 comments comment NNS cord-274424-juj71nc5 192 34 on on IN cord-274424-juj71nc5 192 35 the the DT cord-274424-juj71nc5 192 36 manuscript manuscript NN cord-274424-juj71nc5 192 37 . . . cord-274424-juj71nc5 193 1 This this DT cord-274424-juj71nc5 193 2 research research NN cord-274424-juj71nc5 193 3 was be VBD cord-274424-juj71nc5 193 4 supported support VBN cord-274424-juj71nc5 193 5 by by IN cord-274424-juj71nc5 193 6 the the DT cord-274424-juj71nc5 193 7 Biotechnology Biotechnology NNP cord-274424-juj71nc5 193 8 Action Action NNP cord-274424-juj71nc5 193 9 Programme Programme NNP cord-274424-juj71nc5 193 10 of of IN cord-274424-juj71nc5 193 11 the the DT cord-274424-juj71nc5 193 12 Commission Commission NNP cord-274424-juj71nc5 193 13 of of IN cord-274424-juj71nc5 193 14 the the DT cord-274424-juj71nc5 193 15 European European NNP cord-274424-juj71nc5 193 16 Communities Communities NNPS cord-274424-juj71nc5 193 17 Contract Contract NNP cord-274424-juj71nc5 193 18 N N NNP cord-274424-juj71nc5 193 19 " " '' cord-274424-juj71nc5 193 20 [ [ -LRB- cord-274424-juj71nc5 193 21 BAP-0235.UK(Hl bap-0235.uk(hl NN cord-274424-juj71nc5 193 22 ) ) -RRB- cord-274424-juj71nc5 193 23 ] ] -RRB- cord-274424-juj71nc5 193 24 . . . cord-274424-juj71nc5 194 1 Mice mouse NNS cord-274424-juj71nc5 194 2 immunised immunise VBN cord-274424-juj71nc5 194 3 with with IN cord-274424-juj71nc5 194 4 recombinant recombinant JJ cord-274424-juj71nc5 194 5 vaccinia vaccinia NN cord-274424-juj71nc5 194 6 virus virus NN cord-274424-juj71nc5 194 7 expressing express VBG cord-274424-juj71nc5 194 8 Dengue dengue NN cord-274424-juj71nc5 194 9 4 4 CD cord-274424-juj71nc5 194 10 virus virus NN cord-274424-juj71nc5 194 11 structural structural JJ cord-274424-juj71nc5 194 12 proteins protein NNS cord-274424-juj71nc5 194 13 with with IN cord-274424-juj71nc5 194 14 or or CC cord-274424-juj71nc5 194 15 without without IN cord-274424-juj71nc5 194 16 nonstructural nonstructural JJ cord-274424-juj71nc5 194 17 protein protein NN cord-274424-juj71nc5 194 18 NSl NSl NNS cord-274424-juj71nc5 194 19 are be VBP cord-274424-juj71nc5 194 20 protected protect VBN cord-274424-juj71nc5 194 21 against against IN cord-274424-juj71nc5 194 22 fatal fatal JJ cord-274424-juj71nc5 194 23 Dengue Dengue NNP cord-274424-juj71nc5 194 24 virus virus NN cord-274424-juj71nc5 194 25 encephalitis encephalitis RB cord-274424-juj71nc5 194 26 Sequence sequence NN cord-274424-juj71nc5 194 27 of of IN cord-274424-juj71nc5 194 28 the the DT cord-274424-juj71nc5 194 29 S S NNP cord-274424-juj71nc5 194 30 gene gene NN cord-274424-juj71nc5 194 31 from from IN cord-274424-juj71nc5 194 32 a a DT cord-274424-juj71nc5 194 33 virulent virulent JJ cord-274424-juj71nc5 194 34 British british JJ cord-274424-juj71nc5 194 35 field field NN cord-274424-juj71nc5 194 36 isolate isolate VBP cord-274424-juj71nc5 194 37 of of IN cord-274424-juj71nc5 194 38 transmissible transmissible JJ cord-274424-juj71nc5 194 39 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 194 40 virus virus NN cord-274424-juj71nc5 194 41 Monoclonal monoclonal JJ cord-274424-juj71nc5 194 42 antibodies antibody NNS cord-274424-juj71nc5 194 43 to to IN cord-274424-juj71nc5 194 44 murine murine JJ cord-274424-juj71nc5 194 45 hepatitis hepatitis NN cord-274424-juj71nc5 194 46 virus-4 virus-4 NNP cord-274424-juj71nc5 195 1 ( ( -LRB- cord-274424-juj71nc5 195 2 strain strain NN cord-274424-juj71nc5 195 3 JHM JHM NNP cord-274424-juj71nc5 195 4 ) ) -RRB- cord-274424-juj71nc5 195 5 define define VB cord-274424-juj71nc5 195 6 the the DT cord-274424-juj71nc5 195 7 viral viral JJ cord-274424-juj71nc5 195 8 glycoprotein glycoprotein NN cord-274424-juj71nc5 195 9 responsible responsible JJ cord-274424-juj71nc5 195 10 for for IN cord-274424-juj71nc5 195 11 attachment attachment NN cord-274424-juj71nc5 195 12 and and CC cord-274424-juj71nc5 195 13 cell cell NN cord-274424-juj71nc5 195 14 - - HYPH cord-274424-juj71nc5 195 15 cell cell NN cord-274424-juj71nc5 195 16 fusion fusion NN cord-274424-juj71nc5 195 17 Localization localization NN cord-274424-juj71nc5 195 18 of of IN cord-274424-juj71nc5 195 19 antigenic antigenic JJ cord-274424-juj71nc5 195 20 sites site NNS cord-274424-juj71nc5 195 21 of of IN cord-274424-juj71nc5 195 22 the the DT cord-274424-juj71nc5 195 23 E2 E2 NNP cord-274424-juj71nc5 195 24 glycoprotein glycoprotein NN cord-274424-juj71nc5 195 25 of of IN cord-274424-juj71nc5 195 26 transmissible transmissible JJ cord-274424-juj71nc5 195 27 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 195 28 coronavirus coronavirus NN cord-274424-juj71nc5 195 29 cDNA cdna NN cord-274424-juj71nc5 195 30 cloning cloning NN cord-274424-juj71nc5 195 31 and and CC cord-274424-juj71nc5 195 32 sequence sequence NN cord-274424-juj71nc5 195 33 analysis analysis NN cord-274424-juj71nc5 195 34 of of IN cord-274424-juj71nc5 195 35 the the DT cord-274424-juj71nc5 195 36 gene gene NN cord-274424-juj71nc5 195 37 encoding encode VBG cord-274424-juj71nc5 195 38 the the DT cord-274424-juj71nc5 195 39 peplomer peplomer JJ cord-274424-juj71nc5 195 40 protein protein NN cord-274424-juj71nc5 195 41 of of IN cord-274424-juj71nc5 195 42 feline feline JJ cord-274424-juj71nc5 195 43 infectious infectious JJ cord-274424-juj71nc5 195 44 peritonitis peritonitis NN cord-274424-juj71nc5 195 45 virus virus NN cord-274424-juj71nc5 195 46 Stably stably RB cord-274424-juj71nc5 195 47 expressed express VBD cord-274424-juj71nc5 195 48 FIPV FIPV NNP cord-274424-juj71nc5 195 49 peplomer peplomer NNP cord-274424-juj71nc5 195 50 protein protein NN cord-274424-juj71nc5 195 51 induces induce VBZ cord-274424-juj71nc5 195 52 cell cell NN cord-274424-juj71nc5 195 53 fusion fusion NN cord-274424-juj71nc5 195 54 and and CC cord-274424-juj71nc5 195 55 elicits elicit VBZ cord-274424-juj71nc5 195 56 neutralizing neutralize VBG cord-274424-juj71nc5 195 57 antibodies antibody NNS cord-274424-juj71nc5 195 58 in in IN cord-274424-juj71nc5 195 59 mice mouse NNS cord-274424-juj71nc5 196 1 Four four CD cord-274424-juj71nc5 196 2 major major JJ cord-274424-juj71nc5 196 3 antigenic antigenic JJ cord-274424-juj71nc5 196 4 sites site NNS cord-274424-juj71nc5 196 5 of of IN cord-274424-juj71nc5 196 6 the the DT cord-274424-juj71nc5 196 7 coronavirus coronavirus NN cord-274424-juj71nc5 196 8 transmissible transmissible JJ cord-274424-juj71nc5 196 9 gastroenteritls gastroenteritls NNP cord-274424-juj71nc5 196 10 virus virus NN cord-274424-juj71nc5 196 11 are be VBP cord-274424-juj71nc5 196 12 located locate VBN cord-274424-juj71nc5 196 13 on on IN cord-274424-juj71nc5 196 14 the the DT cord-274424-juj71nc5 196 15 amino amino NN cord-274424-juj71nc5 196 16 - - HYPH cord-274424-juj71nc5 196 17 terminal terminal NN cord-274424-juj71nc5 196 18 half half NN cord-274424-juj71nc5 196 19 of of IN cord-274424-juj71nc5 196 20 spike spike NN cord-274424-juj71nc5 196 21 glycoprotein glycoprotein NN cord-274424-juj71nc5 196 22 S S NNP cord-274424-juj71nc5 196 23 Compartmental Compartmental NNP cord-274424-juj71nc5 196 24 organisation organisation NN cord-274424-juj71nc5 196 25 of of IN cord-274424-juj71nc5 196 26 the the DT cord-274424-juj71nc5 196 27 Golgi Golgi NNP cord-274424-juj71nc5 196 28 stack stack NN cord-274424-juj71nc5 196 29 Pathogenicity Pathogenicity NNP cord-274424-juj71nc5 196 30 of of IN cord-274424-juj71nc5 196 31 antigenic antigenic JJ cord-274424-juj71nc5 196 32 variants variant NNS cord-274424-juj71nc5 196 33 of of IN cord-274424-juj71nc5 196 34 murine murine JJ cord-274424-juj71nc5 196 35 coronavirus coronavirus NN cord-274424-juj71nc5 196 36 JHM JHM NNP cord-274424-juj71nc5 196 37 selected select VBN cord-274424-juj71nc5 196 38 with with IN cord-274424-juj71nc5 196 39 monoclonal monoclonal JJ cord-274424-juj71nc5 196 40 antibodies antibody NNS cord-274424-juj71nc5 197 1 The the DT cord-274424-juj71nc5 197 2 polypeptide polypeptide JJ cord-274424-juj71nc5 197 3 structure structure NN cord-274424-juj71nc5 197 4 of of IN cord-274424-juj71nc5 197 5 transmissible transmissible JJ cord-274424-juj71nc5 197 6 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 197 7 virus virus NN cord-274424-juj71nc5 197 8 /79 /79 . cord-274424-juj71nc5 197 9 ) ) -RRB- cord-274424-juj71nc5 197 10 . . . cord-274424-juj71nc5 198 1 Antigenicity antigenicity NN cord-274424-juj71nc5 198 2 of of IN cord-274424-juj71nc5 198 3 structural structural JJ cord-274424-juj71nc5 198 4 components component NNS cord-274424-juj71nc5 198 5 from from IN cord-274424-juj71nc5 198 6 porcine porcine JJ cord-274424-juj71nc5 198 7 transmissible transmissible JJ cord-274424-juj71nc5 198 8 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 198 9 virus virus NN cord-274424-juj71nc5 198 10 Identification identification NN cord-274424-juj71nc5 198 11 of of IN cord-274424-juj71nc5 198 12 epitopes epitope NNS cord-274424-juj71nc5 198 13 of of IN cord-274424-juj71nc5 198 14 immunological immunological JJ cord-274424-juj71nc5 198 15 importance importance NN cord-274424-juj71nc5 198 16 on on IN cord-274424-juj71nc5 198 17 the the DT cord-274424-juj71nc5 198 18 peplomer peplomer NN cord-274424-juj71nc5 198 19 of of IN cord-274424-juj71nc5 198 20 porcine porcine JJ cord-274424-juj71nc5 198 21 transmissible transmissible JJ cord-274424-juj71nc5 198 22 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 198 23 virus virus NN cord-274424-juj71nc5 198 24 Propagation propagation NN cord-274424-juj71nc5 198 25 of of IN cord-274424-juj71nc5 198 26 the the DT cord-274424-juj71nc5 198 27 porcine porcine JJ cord-274424-juj71nc5 198 28 epidemic epidemic NN cord-274424-juj71nc5 198 29 diarrhoea diarrhoea NN cord-274424-juj71nc5 198 30 in in IN cord-274424-juj71nc5 198 31 cell cell NN cord-274424-juj71nc5 198 32 culture culture NN cord-274424-juj71nc5 198 33 . . . cord-274424-juj71nc5 198 34 /. /. . cord-274424-juj71nc5 199 1 C C NNP cord-274424-juj71nc5 199 2 / / SYM cord-274424-juj71nc5 199 3 in in IN cord-274424-juj71nc5 199 4 . . . cord-274424-juj71nc5 200 1 Microbial microbial JJ cord-274424-juj71nc5 200 2 Cloning cloning NN cord-274424-juj71nc5 200 3 and and CC cord-274424-juj71nc5 200 4 expression expression NN cord-274424-juj71nc5 200 5 of of IN cord-274424-juj71nc5 200 6 the the DT cord-274424-juj71nc5 200 7 surface surface NN cord-274424-juj71nc5 200 8 glycoprotein glycoprotein NN cord-274424-juj71nc5 200 9 gpl95 gpl95 CD cord-274424-juj71nc5 200 10 of of IN cord-274424-juj71nc5 200 11 porcine porcine JJ cord-274424-juj71nc5 200 12 transmissible transmissible JJ cord-274424-juj71nc5 200 13 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 200 14 virus virus NN cord-274424-juj71nc5 200 15 Infectious infectious JJ cord-274424-juj71nc5 200 16 vaccinia vaccinia NN cord-274424-juj71nc5 200 17 virus virus NN cord-274424-juj71nc5 200 18 recombinant recombinant JJ cord-274424-juj71nc5 200 19 that that WDT cord-274424-juj71nc5 200 20 expresses express VBZ cord-274424-juj71nc5 200 21 the the DT cord-274424-juj71nc5 200 22 surface surface NN cord-274424-juj71nc5 200 23 antigen antigen NN cord-274424-juj71nc5 200 24 of of IN cord-274424-juj71nc5 200 25 porcine porcine JJ cord-274424-juj71nc5 200 26 transmissible transmissible JJ cord-274424-juj71nc5 200 27 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 200 28 virus virus NN cord-274424-juj71nc5 200 29 ( ( -LRB- cord-274424-juj71nc5 200 30 TGEV TGEV NNP cord-274424-juj71nc5 200 31 ) ) -RRB- cord-274424-juj71nc5 200 32 . . . cord-274424-juj71nc5 201 1 In in IN cord-274424-juj71nc5 201 2 " " `` cord-274424-juj71nc5 201 3 Vaccinia vaccinia NN cord-274424-juj71nc5 201 4 Viruses virus NNS cord-274424-juj71nc5 201 5 as as IN cord-274424-juj71nc5 201 6 Vectors Vectors NNPS cord-274424-juj71nc5 201 7 for for IN cord-274424-juj71nc5 201 8 Vaccine Vaccine NNP cord-274424-juj71nc5 201 9 Antigens Antigens NNP cord-274424-juj71nc5 201 10 Synthesis Synthesis NNP cord-274424-juj71nc5 201 11 and and CC cord-274424-juj71nc5 201 12 processing processing NN cord-274424-juj71nc5 201 13 of of IN cord-274424-juj71nc5 201 14 asparagine asparagine NN cord-274424-juj71nc5 201 15 linked link VBN cord-274424-juj71nc5 201 16 oligosaccharides oligosaccharide NNS cord-274424-juj71nc5 202 1 The the DT cord-274424-juj71nc5 202 2 nucleotide nucleotide JJ cord-274424-juj71nc5 202 3 sequence sequence NN cord-274424-juj71nc5 202 4 of of IN cord-274424-juj71nc5 202 5 the the DT cord-274424-juj71nc5 202 6 peplomer peplomer JJ cord-274424-juj71nc5 202 7 gene gene NN cord-274424-juj71nc5 202 8 of of IN cord-274424-juj71nc5 202 9 porcine porcine JJ cord-274424-juj71nc5 202 10 transmissible transmissible JJ cord-274424-juj71nc5 202 11 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 202 12 virus virus NN cord-274424-juj71nc5 202 13 ( ( -LRB- cord-274424-juj71nc5 202 14 TGEV TGEV NNP cord-274424-juj71nc5 202 15 ) ) -RRB- cord-274424-juj71nc5 202 16 : : : cord-274424-juj71nc5 203 1 Comparison comparison NN cord-274424-juj71nc5 203 2 with with IN cord-274424-juj71nc5 203 3 the the DT cord-274424-juj71nc5 203 4 sequence sequence NN cord-274424-juj71nc5 203 5 of of IN cord-274424-juj71nc5 203 6 the the DT cord-274424-juj71nc5 203 7 peplomer peplomer JJ cord-274424-juj71nc5 203 8 protein protein NN cord-274424-juj71nc5 203 9 of of IN cord-274424-juj71nc5 203 10 feline feline JJ cord-274424-juj71nc5 203 11 infectious infectious JJ cord-274424-juj71nc5 203 12 peritonitis peritonitis NN cord-274424-juj71nc5 203 13 virus virus NN cord-274424-juj71nc5 203 14 ( ( -LRB- cord-274424-juj71nc5 203 15 FIPV FIPV NNP cord-274424-juj71nc5 203 16 ) ) -RRB- cord-274424-juj71nc5 204 1 Critical critical JJ cord-274424-juj71nc5 204 2 epitopes epitope NNS cord-274424-juj71nc5 204 3 in in IN cord-274424-juj71nc5 204 4 transmissible transmissible JJ cord-274424-juj71nc5 204 5 gastroenteritis gastroenteritis JJ cord-274424-juj71nc5 204 6 virus virus NN cord-274424-juj71nc5 204 7 neutralization neutralization NN cord-274424-juj71nc5 204 8 Cleavage cleavage NN cord-274424-juj71nc5 204 9 of of IN cord-274424-juj71nc5 204 10 structural structural JJ cord-274424-juj71nc5 204 11 proteins protein NNS cord-274424-juj71nc5 204 12 during during IN cord-274424-juj71nc5 204 13 the the DT cord-274424-juj71nc5 204 14 assembly assembly NN cord-274424-juj71nc5 204 15 of of IN cord-274424-juj71nc5 204 16 the the DT cord-274424-juj71nc5 204 17 head head NN cord-274424-juj71nc5 204 18 of of IN cord-274424-juj71nc5 204 19 the the DT cord-274424-juj71nc5 204 20 bacteriophage bacteriophage NN cord-274424-juj71nc5 204 21 T T NNP cord-274424-juj71nc5 204 22 Antigenic Antigenic NNP cord-274424-juj71nc5 204 23 structure structure NN cord-274424-juj71nc5 204 24 of of IN cord-274424-juj71nc5 204 25 transmissible transmissible JJ cord-274424-juj71nc5 204 26 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 204 27 virus virus NN cord-274424-juj71nc5 204 28 . . . cord-274424-juj71nc5 205 1 I. I. NNP cord-274424-juj71nc5 205 2 Properties Properties NNP cord-274424-juj71nc5 205 3 of of IN cord-274424-juj71nc5 205 4 monoclonal monoclonal JJ cord-274424-juj71nc5 205 5 antibodies antibody NNS cord-274424-juj71nc5 205 6 directed direct VBN cord-274424-juj71nc5 205 7 against against IN cord-274424-juj71nc5 205 8 virion virion NN cord-274424-juj71nc5 205 9 proteins protein NNS cord-274424-juj71nc5 205 10 Transport Transport NNP cord-274424-juj71nc5 205 11 of of IN cord-274424-juj71nc5 205 12 secretory secretory NN cord-274424-juj71nc5 205 13 and and CC cord-274424-juj71nc5 205 14 membrane membrane NN cord-274424-juj71nc5 205 15 glycoproteins glycoprotein NNS cord-274424-juj71nc5 205 16 from from IN cord-274424-juj71nc5 205 17 the the DT cord-274424-juj71nc5 205 18 rough rough JJ cord-274424-juj71nc5 205 19 endoplasmic endoplasmic NN cord-274424-juj71nc5 205 20 reticulum reticulum NN cord-274424-juj71nc5 205 21 to to IN cord-274424-juj71nc5 205 22 the the DT cord-274424-juj71nc5 205 23 Golgi Golgi NNP cord-274424-juj71nc5 205 24 General General NNP cord-274424-juj71nc5 205 25 method method NN cord-274424-juj71nc5 205 26 for for IN cord-274424-juj71nc5 205 27 production production NN cord-274424-juj71nc5 205 28 and and CC cord-274424-juj71nc5 205 29 selection selection NN cord-274424-juj71nc5 205 30 of of IN cord-274424-juj71nc5 205 31 infectious infectious JJ cord-274424-juj71nc5 205 32 vaccinia vaccinia NN cord-274424-juj71nc5 205 33 virus virus NN cord-274424-juj71nc5 205 34 recombinants recombinant NNS cord-274424-juj71nc5 205 35 expressing express VBG cord-274424-juj71nc5 205 36 foreign foreign JJ cord-274424-juj71nc5 205 37 genes gene NNS cord-274424-juj71nc5 206 1 The the DT cord-274424-juj71nc5 206 2 construction construction NN cord-274424-juj71nc5 206 3 and and CC cord-274424-juj71nc5 206 4 characterisation characterisation NN cord-274424-juj71nc5 206 5 of of IN cord-274424-juj71nc5 206 6 vaccinia vaccinia NN cord-274424-juj71nc5 206 7 virus virus NN cord-274424-juj71nc5 206 8 recombinants recombinant NNS cord-274424-juj71nc5 206 9 expressing express VBG cord-274424-juj71nc5 206 10 foreign foreign JJ cord-274424-juj71nc5 206 11 genes gene NNS cord-274424-juj71nc5 206 12 . . . cord-274424-juj71nc5 207 1 ln ln JJ cord-274424-juj71nc5 207 2 " " `` cord-274424-juj71nc5 207 3 DNA DNA NNP cord-274424-juj71nc5 207 4 Cloning Cloning NNP cord-274424-juj71nc5 207 5 , , , cord-274424-juj71nc5 207 6 a a DT cord-274424-juj71nc5 207 7 Practical Practical NNP cord-274424-juj71nc5 207 8 Approach Approach NNP cord-274424-juj71nc5 207 9 Molecular Molecular NNP cord-274424-juj71nc5 207 10 Cloning cloning NN cord-274424-juj71nc5 207 11 : : : cord-274424-juj71nc5 207 12 A a DT cord-274424-juj71nc5 207 13 Laboratory Laboratory NNP cord-274424-juj71nc5 207 14 Manual Manual NNP cord-274424-juj71nc5 207 15 Post Post NNP cord-274424-juj71nc5 207 16 - - JJ cord-274424-juj71nc5 207 17 translational translational JJ cord-274424-juj71nc5 207 18 glycosylation glycosylation NN cord-274424-juj71nc5 207 19 of of IN cord-274424-juj71nc5 207 20 glycoprotein glycoprotein NN cord-274424-juj71nc5 207 21 El El NNP cord-274424-juj71nc5 207 22 : : : cord-274424-juj71nc5 208 1 Inhibition inhibition NN cord-274424-juj71nc5 208 2 by by IN cord-274424-juj71nc5 208 3 monensin monensin NNP cord-274424-juj71nc5 208 4 Cytoplasmic Cytoplasmic NNP cord-274424-juj71nc5 208 5 domains domain NNS cord-274424-juj71nc5 208 6 of of IN cord-274424-juj71nc5 208 7 cellular cellular JJ cord-274424-juj71nc5 208 8 and and CC cord-274424-juj71nc5 208 9 viral viral JJ cord-274424-juj71nc5 208 10 integral integral JJ cord-274424-juj71nc5 208 11 membrane membrane NN cord-274424-juj71nc5 208 12 proteins protein NNS cord-274424-juj71nc5 208 13 substatute substatute VBP cord-274424-juj71nc5 208 14 for for IN cord-274424-juj71nc5 208 15 the the DT cord-274424-juj71nc5 208 16 cytoplasmic cytoplasmic JJ cord-274424-juj71nc5 208 17 domain domain NN cord-274424-juj71nc5 208 18 of of IN cord-274424-juj71nc5 208 19 the the DT cord-274424-juj71nc5 208 20 vesicular vesicular JJ cord-274424-juj71nc5 208 21 stomatitis stomatitis NN cord-274424-juj71nc5 208 22 virus virus NN cord-274424-juj71nc5 208 23 glycoprotein glycoprotein NN cord-274424-juj71nc5 208 24 in in IN cord-274424-juj71nc5 208 25 transport transport NN cord-274424-juj71nc5 208 26 to to IN cord-274424-juj71nc5 208 27 the the DT cord-274424-juj71nc5 208 28 plasma plasma NN cord-274424-juj71nc5 208 29 membrane membrane NN cord-274424-juj71nc5 208 30 Expression expression NN cord-274424-juj71nc5 208 31 of of IN cord-274424-juj71nc5 208 32 TGEV TGEV NNP cord-274424-juj71nc5 208 33 structural structural JJ cord-274424-juj71nc5 208 34 genes gene NNS cord-274424-juj71nc5 208 35 in in IN cord-274424-juj71nc5 208 36 virus virus NN cord-274424-juj71nc5 208 37 vectors vector VBZ cord-274424-juj71nc5 208 38 Expression expression NN cord-274424-juj71nc5 208 39 and and CC cord-274424-juj71nc5 208 40 cellular cellular JJ cord-274424-juj71nc5 208 41 localisation localisation NN cord-274424-juj71nc5 208 42 of of IN cord-274424-juj71nc5 208 43 porcine porcine JJ cord-274424-juj71nc5 208 44 transmissible transmissible JJ cord-274424-juj71nc5 208 45 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 208 46 virus virus NN cord-274424-juj71nc5 208 47 N N NNP cord-274424-juj71nc5 208 48 and and CC cord-274424-juj71nc5 208 49 M M NNP cord-274424-juj71nc5 208 50 proteins protein NNS cord-274424-juj71nc5 208 51 by by IN cord-274424-juj71nc5 208 52 recombinant recombinant JJ cord-274424-juj71nc5 208 53 vaccinia vaccinia NN cord-274424-juj71nc5 208 54 viruses virus NNS cord-274424-juj71nc5 209 1 The the DT cord-274424-juj71nc5 209 2 predicted predict VBN cord-274424-juj71nc5 209 3 structure structure NN cord-274424-juj71nc5 209 4 of of IN cord-274424-juj71nc5 209 5 the the DT cord-274424-juj71nc5 209 6 peplomer peplomer NNP cord-274424-juj71nc5 209 7 protein protein NN cord-274424-juj71nc5 209 8 E2 E2 NNP cord-274424-juj71nc5 209 9 of of IN cord-274424-juj71nc5 209 10 the the DT cord-274424-juj71nc5 209 11 porcine porcine JJ cord-274424-juj71nc5 209 12 coronavirus coronavirus NN cord-274424-juj71nc5 209 13 transmissible transmissible JJ cord-274424-juj71nc5 209 14 gastroenteritis gastroenteritis NN cord-274424-juj71nc5 209 15 virus virus NN cord-274424-juj71nc5 209 16 Coronaviruses Coronaviruses NNP cord-274424-juj71nc5 209 17 : : : cord-274424-juj71nc5 210 1 Structure structure NN cord-274424-juj71nc5 210 2 and and CC cord-274424-juj71nc5 210 3 genome genome NN cord-274424-juj71nc5 210 4 expression expression NN cord-274424-juj71nc5 211 1 Proteolytic proteolytic JJ cord-274424-juj71nc5 211 2 cleavage cleavage NN cord-274424-juj71nc5 211 3 of of IN cord-274424-juj71nc5 211 4 the the DT cord-274424-juj71nc5 211 5 E2 E2 NNP cord-274424-juj71nc5 211 6 glycoprotein glycoprotein NN cord-274424-juj71nc5 211 7 of of IN cord-274424-juj71nc5 211 8 murine murine JJ cord-274424-juj71nc5 211 9 coronavirus coronavirus NN cord-274424-juj71nc5 211 10 : : : cord-274424-juj71nc5 211 11 Activation activation NN cord-274424-juj71nc5 211 12 of of IN cord-274424-juj71nc5 211 13 cell cell NN cord-274424-juj71nc5 211 14 - - HYPH cord-274424-juj71nc5 211 15 fusing fuse VBG cord-274424-juj71nc5 211 16 activity activity NN cord-274424-juj71nc5 211 17 of of IN cord-274424-juj71nc5 211 18 virions virion NNS cord-274424-juj71nc5 211 19 by by IN cord-274424-juj71nc5 211 20 ttypsin ttypsin NNP cord-274424-juj71nc5 211 21 and and CC cord-274424-juj71nc5 211 22 separation separation NN cord-274424-juj71nc5 211 23 of of IN cord-274424-juj71nc5 211 24 two two CD cord-274424-juj71nc5 211 25 different different JJ cord-274424-juj71nc5 211 26 90 90 CD cord-274424-juj71nc5 211 27 K k NN cord-274424-juj71nc5 211 28 cleavage cleavage NN cord-274424-juj71nc5 211 29 fragments fragment NNS cord-274424-juj71nc5 211 30 Cell cell NN cord-274424-juj71nc5 211 31 surface surface NN cord-274424-juj71nc5 211 32 expression expression NN cord-274424-juj71nc5 211 33 of of IN cord-274424-juj71nc5 211 34 the the DT cord-274424-juj71nc5 211 35 influenza influenza NN cord-274424-juj71nc5 211 36 virus virus NN cord-274424-juj71nc5 211 37 haemagglutinin haemagglutinin NNP cord-274424-juj71nc5 211 38 requires require VBZ cord-274424-juj71nc5 211 39 the the DT cord-274424-juj71nc5 211 40 hydrophobic hydrophobic JJ cord-274424-juj71nc5 211 41 carboxy carboxy JJ cord-274424-juj71nc5 211 42 - - HYPH cord-274424-juj71nc5 211 43 terminal terminal NN cord-274424-juj71nc5 211 44 sequences sequence NNS cord-274424-juj71nc5 211 45 Laminated laminate VBN cord-274424-juj71nc5 211 46 cisternae cisternae NNP cord-274424-juj71nc5 211 47 of of IN cord-274424-juj71nc5 211 48 the the DT cord-274424-juj71nc5 211 49 rough rough JJ cord-274424-juj71nc5 211 50 endoplasmic endoplasmic NN cord-274424-juj71nc5 211 51 reticulum reticulum NN cord-274424-juj71nc5 211 52 induced induce VBN cord-274424-juj71nc5 211 53 by by IN cord-274424-juj71nc5 211 54 coronavirus coronavirus NN cord-274424-juj71nc5 211 55 MHV MHV NNP cord-274424-juj71nc5 211 56 - - HYPH cord-274424-juj71nc5 211 57 A59 A59 NNP cord-274424-juj71nc5 211 58 infection infection NN cord-274424-juj71nc5 212 1 Intracellular intracellular JJ cord-274424-juj71nc5 212 2 transport transport NN cord-274424-juj71nc5 212 3 of of IN cord-274424-juj71nc5 212 4 recombinant recombinant JJ cord-274424-juj71nc5 212 5 coronavirus coronavirus NN cord-274424-juj71nc5 212 6 spike spike NN cord-274424-juj71nc5 212 7 proteins protein NNS cord-274424-juj71nc5 212 8 : : : cord-274424-juj71nc5 212 9 Implications implication NNS cord-274424-juj71nc5 212 10 for for IN cord-274424-juj71nc5 212 11 virus virus NN cord-274424-juj71nc5 212 12 assembly assembly NN