id sid tid token lemma pos cord-275664-qbafkxtr 1 1 key key NN cord-275664-qbafkxtr 1 2 : : : cord-275664-qbafkxtr 2 1 cord-275664-qbafkxtr cord-275664-qbafkxtr NNP cord-275664-qbafkxtr 3 1 authors author NNS cord-275664-qbafkxtr 3 2 : : : cord-275664-qbafkxtr 3 3 Wei Wei NNP cord-275664-qbafkxtr 3 4 , , , cord-275664-qbafkxtr 3 5 Li Li NNP cord-275664-qbafkxtr 3 6 ; ; : cord-275664-qbafkxtr 3 7 Liu Liu NNP cord-275664-qbafkxtr 3 8 , , , cord-275664-qbafkxtr 4 1 Jue Jue NNP cord-275664-qbafkxtr 4 2 title title NN cord-275664-qbafkxtr 4 3 : : : cord-275664-qbafkxtr 5 1 Porcine porcine JJ cord-275664-qbafkxtr 5 2 circovirus circovirus JJ cord-275664-qbafkxtr 5 3 type type NN cord-275664-qbafkxtr 5 4 2 2 CD cord-275664-qbafkxtr 5 5 replication replication NN cord-275664-qbafkxtr 5 6 is be VBZ cord-275664-qbafkxtr 5 7 impaired impair VBN cord-275664-qbafkxtr 5 8 by by IN cord-275664-qbafkxtr 5 9 inhibition inhibition NN cord-275664-qbafkxtr 5 10 of of IN cord-275664-qbafkxtr 5 11 the the DT cord-275664-qbafkxtr 5 12 extracellular extracellular JJ cord-275664-qbafkxtr 5 13 signal signal NN cord-275664-qbafkxtr 5 14 - - HYPH cord-275664-qbafkxtr 5 15 regulated regulate VBN cord-275664-qbafkxtr 5 16 kinase kinase NN cord-275664-qbafkxtr 5 17 ( ( -LRB- cord-275664-qbafkxtr 5 18 ERK ERK NNP cord-275664-qbafkxtr 5 19 ) ) -RRB- cord-275664-qbafkxtr 5 20 signaling signal VBG cord-275664-qbafkxtr 5 21 pathway pathway NN cord-275664-qbafkxtr 5 22 date date NN cord-275664-qbafkxtr 5 23 : : : cord-275664-qbafkxtr 5 24 2009 2009 CD cord-275664-qbafkxtr 5 25 - - SYM cord-275664-qbafkxtr 5 26 03 03 CD cord-275664-qbafkxtr 5 27 - - SYM cord-275664-qbafkxtr 5 28 30 30 CD cord-275664-qbafkxtr 5 29 journal journal NN cord-275664-qbafkxtr 5 30 : : : cord-275664-qbafkxtr 5 31 Virology Virology NNP cord-275664-qbafkxtr 5 32 DOI DOI NNP cord-275664-qbafkxtr 5 33 : : : cord-275664-qbafkxtr 5 34 10.1016 10.1016 CD cord-275664-qbafkxtr 6 1 /j.virol.2009.01.010 /j.virol.2009.01.010 NFP cord-275664-qbafkxtr 6 2 sha sha NNP cord-275664-qbafkxtr 6 3 : : : cord-275664-qbafkxtr 6 4 73fd3edae7eff2a590a42dd7e744698881448511 73fd3edae7eff2a590a42dd7e744698881448511 CD cord-275664-qbafkxtr 6 5 doc_id doc_id CD cord-275664-qbafkxtr 6 6 : : : cord-275664-qbafkxtr 6 7 275664 275664 CD cord-275664-qbafkxtr 6 8 cord_uid cord_uid NNS cord-275664-qbafkxtr 6 9 : : : cord-275664-qbafkxtr 7 1 qbafkxtr qbafkxtr NNP cord-275664-qbafkxtr 7 2 Postweaning Postweaning NNP cord-275664-qbafkxtr 7 3 multisystemic multisystemic NNP cord-275664-qbafkxtr 7 4 wasting waste VBG cord-275664-qbafkxtr 7 5 syndrome syndrome NN cord-275664-qbafkxtr 7 6 , , , cord-275664-qbafkxtr 7 7 which which WDT cord-275664-qbafkxtr 7 8 is be VBZ cord-275664-qbafkxtr 7 9 primarily primarily RB cord-275664-qbafkxtr 7 10 caused cause VBN cord-275664-qbafkxtr 7 11 by by IN cord-275664-qbafkxtr 7 12 porcine porcine JJ cord-275664-qbafkxtr 7 13 circovirus circovirus NNP cord-275664-qbafkxtr 7 14 type type NN cord-275664-qbafkxtr 7 15 2 2 CD cord-275664-qbafkxtr 8 1 ( ( -LRB- cord-275664-qbafkxtr 8 2 PCV2 PCV2 NNP cord-275664-qbafkxtr 8 3 ) ) -RRB- cord-275664-qbafkxtr 8 4 , , , cord-275664-qbafkxtr 8 5 is be VBZ cord-275664-qbafkxtr 8 6 an an DT cord-275664-qbafkxtr 8 7 emerging emerge VBG cord-275664-qbafkxtr 8 8 and and CC cord-275664-qbafkxtr 8 9 important important JJ cord-275664-qbafkxtr 8 10 swine swine NN cord-275664-qbafkxtr 8 11 disease disease NN cord-275664-qbafkxtr 8 12 . . . cord-275664-qbafkxtr 9 1 We -PRON- PRP cord-275664-qbafkxtr 9 2 have have VBP cord-275664-qbafkxtr 9 3 recently recently RB cord-275664-qbafkxtr 9 4 shown show VBN cord-275664-qbafkxtr 9 5 that that IN cord-275664-qbafkxtr 9 6 PCV2 PCV2 NNP cord-275664-qbafkxtr 9 7 induces induce VBZ cord-275664-qbafkxtr 9 8 nuclear nuclear JJ cord-275664-qbafkxtr 9 9 factor factor NN cord-275664-qbafkxtr 9 10 kappa kappa NNP cord-275664-qbafkxtr 9 11 B B NNP cord-275664-qbafkxtr 9 12 activation activation NN cord-275664-qbafkxtr 9 13 and and CC cord-275664-qbafkxtr 9 14 its -PRON- PRP$ cord-275664-qbafkxtr 9 15 activation activation NN cord-275664-qbafkxtr 9 16 is be VBZ cord-275664-qbafkxtr 9 17 required require VBN cord-275664-qbafkxtr 9 18 for for IN cord-275664-qbafkxtr 9 19 active active JJ cord-275664-qbafkxtr 9 20 replication replication NN cord-275664-qbafkxtr 9 21 , , , cord-275664-qbafkxtr 9 22 but but CC cord-275664-qbafkxtr 9 23 the the DT cord-275664-qbafkxtr 9 24 other other JJ cord-275664-qbafkxtr 9 25 cellular cellular JJ cord-275664-qbafkxtr 9 26 factors factor NNS cord-275664-qbafkxtr 9 27 involved involve VBN cord-275664-qbafkxtr 9 28 in in IN cord-275664-qbafkxtr 9 29 PCV2 pcv2 JJ cord-275664-qbafkxtr 9 30 replication replication NN cord-275664-qbafkxtr 9 31 are be VBP cord-275664-qbafkxtr 9 32 not not RB cord-275664-qbafkxtr 9 33 well well RB cord-275664-qbafkxtr 9 34 defined define VBN cord-275664-qbafkxtr 9 35 . . . cord-275664-qbafkxtr 10 1 The the DT cord-275664-qbafkxtr 10 2 extracellular extracellular JJ cord-275664-qbafkxtr 10 3 signal signal NN cord-275664-qbafkxtr 10 4 - - HYPH cord-275664-qbafkxtr 10 5 regulated regulate VBN cord-275664-qbafkxtr 10 6 kinase kinase NN cord-275664-qbafkxtr 10 7 ( ( -LRB- cord-275664-qbafkxtr 10 8 ERK ERK NNP cord-275664-qbafkxtr 10 9 ) ) -RRB- cord-275664-qbafkxtr 10 10 which which WDT cord-275664-qbafkxtr 10 11 served serve VBD cord-275664-qbafkxtr 10 12 as as IN cord-275664-qbafkxtr 10 13 an an DT cord-275664-qbafkxtr 10 14 important important JJ cord-275664-qbafkxtr 10 15 component component NN cord-275664-qbafkxtr 10 16 of of IN cord-275664-qbafkxtr 10 17 cellular cellular JJ cord-275664-qbafkxtr 10 18 signal signal NN cord-275664-qbafkxtr 10 19 transduction transduction NN cord-275664-qbafkxtr 10 20 pathways pathway NNS cord-275664-qbafkxtr 10 21 has have VBZ cord-275664-qbafkxtr 10 22 been be VBN cord-275664-qbafkxtr 10 23 shown show VBN cord-275664-qbafkxtr 10 24 to to TO cord-275664-qbafkxtr 10 25 regulate regulate VB cord-275664-qbafkxtr 10 26 many many JJ cord-275664-qbafkxtr 10 27 viral viral JJ cord-275664-qbafkxtr 10 28 infections infection NNS cord-275664-qbafkxtr 10 29 . . . cord-275664-qbafkxtr 11 1 In in IN cord-275664-qbafkxtr 11 2 this this DT cord-275664-qbafkxtr 11 3 report report NN cord-275664-qbafkxtr 11 4 , , , cord-275664-qbafkxtr 11 5 we -PRON- PRP cord-275664-qbafkxtr 11 6 show show VBP cord-275664-qbafkxtr 11 7 that that IN cord-275664-qbafkxtr 11 8 PCV2 PCV2 NNP cord-275664-qbafkxtr 11 9 activates activate VBZ cord-275664-qbafkxtr 11 10 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 11 11 in in IN cord-275664-qbafkxtr 11 12 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 11 13 PK15 PK15 NNP cord-275664-qbafkxtr 11 14 cells cell NNS cord-275664-qbafkxtr 11 15 dependent dependent JJ cord-275664-qbafkxtr 11 16 on on IN cord-275664-qbafkxtr 11 17 viral viral JJ cord-275664-qbafkxtr 11 18 replication replication NN cord-275664-qbafkxtr 11 19 . . . cord-275664-qbafkxtr 12 1 The the DT cord-275664-qbafkxtr 12 2 PCV2-induced pcv2-induced JJ cord-275664-qbafkxtr 12 3 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 12 4 leads lead VBZ cord-275664-qbafkxtr 12 5 to to IN cord-275664-qbafkxtr 12 6 phosphorylation phosphorylation NN cord-275664-qbafkxtr 12 7 of of IN cord-275664-qbafkxtr 12 8 the the DT cord-275664-qbafkxtr 12 9 ternary ternary JJ cord-275664-qbafkxtr 12 10 complex complex JJ cord-275664-qbafkxtr 12 11 factor factor NN cord-275664-qbafkxtr 12 12 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 12 13 , , , cord-275664-qbafkxtr 12 14 which which WDT cord-275664-qbafkxtr 12 15 kinetically kinetically RB cord-275664-qbafkxtr 12 16 paralleled parallel VBD cord-275664-qbafkxtr 12 17 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 12 18 activation activation NN cord-275664-qbafkxtr 12 19 . . . cord-275664-qbafkxtr 13 1 Inhibition inhibition NN cord-275664-qbafkxtr 13 2 of of IN cord-275664-qbafkxtr 13 3 ERK ERK NNP cord-275664-qbafkxtr 13 4 activation activation NN cord-275664-qbafkxtr 13 5 with with IN cord-275664-qbafkxtr 13 6 U0126 U0126 NNP cord-275664-qbafkxtr 13 7 , , , cord-275664-qbafkxtr 13 8 a a DT cord-275664-qbafkxtr 13 9 specific specific JJ cord-275664-qbafkxtr 13 10 MEK1/2 MEK1/2 NNP cord-275664-qbafkxtr 13 11 inhibitor inhibitor NN cord-275664-qbafkxtr 13 12 , , , cord-275664-qbafkxtr 13 13 significantly significantly RB cord-275664-qbafkxtr 13 14 reduced reduce VBN cord-275664-qbafkxtr 13 15 viral viral JJ cord-275664-qbafkxtr 13 16 progeny progeny NN cord-275664-qbafkxtr 13 17 release release NN cord-275664-qbafkxtr 13 18 . . . cord-275664-qbafkxtr 14 1 Investigations investigation NNS cord-275664-qbafkxtr 14 2 into into IN cord-275664-qbafkxtr 14 3 the the DT cord-275664-qbafkxtr 14 4 mechanism mechanism NN cord-275664-qbafkxtr 14 5 of of IN cord-275664-qbafkxtr 14 6 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 14 7 regulation regulation NN cord-275664-qbafkxtr 14 8 revealed reveal VBD cord-275664-qbafkxtr 14 9 that that IN cord-275664-qbafkxtr 14 10 inhibition inhibition NN cord-275664-qbafkxtr 14 11 of of IN cord-275664-qbafkxtr 14 12 ERK ERK NNP cord-275664-qbafkxtr 14 13 activation activation NN cord-275664-qbafkxtr 14 14 leads lead VBZ cord-275664-qbafkxtr 14 15 to to IN cord-275664-qbafkxtr 14 16 decreased decrease VBN cord-275664-qbafkxtr 14 17 viral viral JJ cord-275664-qbafkxtr 14 18 transcription transcription NN cord-275664-qbafkxtr 14 19 and and CC cord-275664-qbafkxtr 14 20 lower low JJR cord-275664-qbafkxtr 14 21 virus virus NN cord-275664-qbafkxtr 14 22 protein protein NN cord-275664-qbafkxtr 14 23 expression expression NN cord-275664-qbafkxtr 14 24 . . . cord-275664-qbafkxtr 15 1 These these DT cord-275664-qbafkxtr 15 2 data datum NNS cord-275664-qbafkxtr 15 3 indicate indicate VBP cord-275664-qbafkxtr 15 4 that that IN cord-275664-qbafkxtr 15 5 the the DT cord-275664-qbafkxtr 15 6 ERK ERK NNP cord-275664-qbafkxtr 15 7 signaling signal VBG cord-275664-qbafkxtr 15 8 pathway pathway NN cord-275664-qbafkxtr 15 9 is be VBZ cord-275664-qbafkxtr 15 10 involved involve VBN cord-275664-qbafkxtr 15 11 in in IN cord-275664-qbafkxtr 15 12 PCV2 pcv2 JJ cord-275664-qbafkxtr 15 13 infection infection NN cord-275664-qbafkxtr 15 14 and and CC cord-275664-qbafkxtr 15 15 beneficial beneficial JJ cord-275664-qbafkxtr 15 16 to to IN cord-275664-qbafkxtr 15 17 PCV2 pcv2 JJ cord-275664-qbafkxtr 15 18 replication replication NN cord-275664-qbafkxtr 15 19 in in IN cord-275664-qbafkxtr 15 20 the the DT cord-275664-qbafkxtr 15 21 cultured cultured JJ cord-275664-qbafkxtr 15 22 cells cell NNS cord-275664-qbafkxtr 15 23 . . . cord-275664-qbafkxtr 16 1 Porcine porcine JJ cord-275664-qbafkxtr 16 2 circovirus circovirus NN cord-275664-qbafkxtr 16 3 ( ( -LRB- cord-275664-qbafkxtr 16 4 PCV pcv NN cord-275664-qbafkxtr 16 5 ) ) -RRB- cord-275664-qbafkxtr 16 6 is be VBZ cord-275664-qbafkxtr 16 7 classified classify VBN cord-275664-qbafkxtr 16 8 in in IN cord-275664-qbafkxtr 16 9 the the DT cord-275664-qbafkxtr 16 10 genus genus NN cord-275664-qbafkxtr 16 11 Circovirus Circovirus NNP cord-275664-qbafkxtr 16 12 of of IN cord-275664-qbafkxtr 16 13 the the DT cord-275664-qbafkxtr 16 14 family family NN cord-275664-qbafkxtr 16 15 Circoviridae Circoviridae NNP cord-275664-qbafkxtr 17 1 ( ( -LRB- cord-275664-qbafkxtr 17 2 Todd Todd NNP cord-275664-qbafkxtr 17 3 et et NNP cord-275664-qbafkxtr 17 4 al al NNP cord-275664-qbafkxtr 17 5 . . NNP cord-275664-qbafkxtr 17 6 , , , cord-275664-qbafkxtr 17 7 2005 2005 CD cord-275664-qbafkxtr 17 8 ) ) -RRB- cord-275664-qbafkxtr 17 9 . . . cord-275664-qbafkxtr 18 1 Two two CD cord-275664-qbafkxtr 18 2 genotypes genotype NNS cord-275664-qbafkxtr 18 3 of of IN cord-275664-qbafkxtr 18 4 PCV PCV NNP cord-275664-qbafkxtr 18 5 have have VBP cord-275664-qbafkxtr 18 6 been be VBN cord-275664-qbafkxtr 18 7 identified identify VBN cord-275664-qbafkxtr 18 8 . . . cord-275664-qbafkxtr 19 1 PCV PCV NNP cord-275664-qbafkxtr 19 2 type type NN cord-275664-qbafkxtr 19 3 1 1 CD cord-275664-qbafkxtr 19 4 ( ( -LRB- cord-275664-qbafkxtr 19 5 PCV1 PCV1 NNP cord-275664-qbafkxtr 19 6 ) ) -RRB- cord-275664-qbafkxtr 19 7 , , , cord-275664-qbafkxtr 19 8 which which WDT cord-275664-qbafkxtr 19 9 was be VBD cord-275664-qbafkxtr 19 10 first first RB cord-275664-qbafkxtr 19 11 recognized recognize VBN cord-275664-qbafkxtr 19 12 in in IN cord-275664-qbafkxtr 19 13 1974 1974 CD cord-275664-qbafkxtr 19 14 as as IN cord-275664-qbafkxtr 19 15 a a DT cord-275664-qbafkxtr 19 16 contaminant contaminant NN cord-275664-qbafkxtr 19 17 of of IN cord-275664-qbafkxtr 19 18 a a DT cord-275664-qbafkxtr 19 19 continuous continuous JJ cord-275664-qbafkxtr 19 20 porcine porcine JJ cord-275664-qbafkxtr 19 21 kidney kidney NN cord-275664-qbafkxtr 19 22 cell cell NN cord-275664-qbafkxtr 19 23 line line NN cord-275664-qbafkxtr 19 24 ( ( -LRB- cord-275664-qbafkxtr 19 25 PK15 PK15 NNP cord-275664-qbafkxtr 19 26 ) ) -RRB- cord-275664-qbafkxtr 20 1 ( ( -LRB- cord-275664-qbafkxtr 20 2 Tischer Tischer NNP cord-275664-qbafkxtr 20 3 et et NNP cord-275664-qbafkxtr 20 4 al al NNP cord-275664-qbafkxtr 20 5 . . NNP cord-275664-qbafkxtr 20 6 , , , cord-275664-qbafkxtr 20 7 1982 1982 CD cord-275664-qbafkxtr 20 8 ) ) -RRB- cord-275664-qbafkxtr 20 9 , , , cord-275664-qbafkxtr 20 10 is be VBZ cord-275664-qbafkxtr 20 11 known know VBN cord-275664-qbafkxtr 20 12 to to TO cord-275664-qbafkxtr 20 13 be be VB cord-275664-qbafkxtr 20 14 non non JJ cord-275664-qbafkxtr 20 15 - - JJ cord-275664-qbafkxtr 20 16 pathogenic pathogenic JJ cord-275664-qbafkxtr 20 17 to to IN cord-275664-qbafkxtr 20 18 pigs pig NNS cord-275664-qbafkxtr 20 19 ( ( -LRB- cord-275664-qbafkxtr 20 20 Allan Allan NNP cord-275664-qbafkxtr 20 21 et et NNP cord-275664-qbafkxtr 20 22 al al NNP cord-275664-qbafkxtr 20 23 . . NNP cord-275664-qbafkxtr 20 24 , , , cord-275664-qbafkxtr 20 25 1995 1995 CD cord-275664-qbafkxtr 20 26 ) ) -RRB- cord-275664-qbafkxtr 20 27 . . . cord-275664-qbafkxtr 21 1 Infection infection NN cord-275664-qbafkxtr 21 2 with with IN cord-275664-qbafkxtr 21 3 PCV pcv NN cord-275664-qbafkxtr 21 4 type type NN cord-275664-qbafkxtr 21 5 2 2 CD cord-275664-qbafkxtr 22 1 ( ( -LRB- cord-275664-qbafkxtr 22 2 PCV2 PCV2 NNP cord-275664-qbafkxtr 22 3 ) ) -RRB- cord-275664-qbafkxtr 22 4 has have VBZ cord-275664-qbafkxtr 22 5 been be VBN cord-275664-qbafkxtr 22 6 associated associate VBN cord-275664-qbafkxtr 22 7 with with IN cord-275664-qbafkxtr 22 8 postweaning postweane VBG cord-275664-qbafkxtr 22 9 multisystemic multisystemic NNP cord-275664-qbafkxtr 22 10 wasting waste VBG cord-275664-qbafkxtr 22 11 syndrome syndrome NN cord-275664-qbafkxtr 22 12 ( ( -LRB- cord-275664-qbafkxtr 22 13 PMWS PMWS NNP cord-275664-qbafkxtr 22 14 ) ) -RRB- cord-275664-qbafkxtr 23 1 in in IN cord-275664-qbafkxtr 23 2 young young JJ cord-275664-qbafkxtr 23 3 weaned wean VBN cord-275664-qbafkxtr 23 4 pigs pig NNS cord-275664-qbafkxtr 23 5 , , , cord-275664-qbafkxtr 23 6 which which WDT cord-275664-qbafkxtr 23 7 was be VBD cord-275664-qbafkxtr 23 8 first first RB cord-275664-qbafkxtr 23 9 recognized recognize VBN cord-275664-qbafkxtr 23 10 in in IN cord-275664-qbafkxtr 23 11 Canada Canada NNP cord-275664-qbafkxtr 23 12 in in IN cord-275664-qbafkxtr 23 13 1991 1991 CD cord-275664-qbafkxtr 23 14 ( ( -LRB- cord-275664-qbafkxtr 23 15 Clark Clark NNP cord-275664-qbafkxtr 23 16 , , , cord-275664-qbafkxtr 23 17 1997 1997 CD cord-275664-qbafkxtr 23 18 ) ) -RRB- cord-275664-qbafkxtr 23 19 . . . cord-275664-qbafkxtr 24 1 Nowadays nowadays RB cord-275664-qbafkxtr 24 2 , , , cord-275664-qbafkxtr 24 3 this this DT cord-275664-qbafkxtr 24 4 disease disease NN cord-275664-qbafkxtr 24 5 and and CC cord-275664-qbafkxtr 24 6 related relate VBN cord-275664-qbafkxtr 24 7 PCV2-associated pcv2-associated JJ cord-275664-qbafkxtr 24 8 diseases disease NNS cord-275664-qbafkxtr 24 9 are be VBP cord-275664-qbafkxtr 24 10 occurring occur VBG cord-275664-qbafkxtr 24 11 in in IN cord-275664-qbafkxtr 24 12 all all DT cord-275664-qbafkxtr 24 13 swine swine NN cord-275664-qbafkxtr 24 14 - - HYPH cord-275664-qbafkxtr 24 15 producing produce VBG cord-275664-qbafkxtr 24 16 areas area NNS cord-275664-qbafkxtr 24 17 of of IN cord-275664-qbafkxtr 24 18 the the DT cord-275664-qbafkxtr 24 19 world world NN cord-275664-qbafkxtr 24 20 and and CC cord-275664-qbafkxtr 24 21 have have VBP cord-275664-qbafkxtr 24 22 become become VBN cord-275664-qbafkxtr 24 23 increasingly increasingly RB cord-275664-qbafkxtr 24 24 serious serious JJ cord-275664-qbafkxtr 24 25 threats threat NNS cord-275664-qbafkxtr 24 26 to to IN cord-275664-qbafkxtr 24 27 global global JJ cord-275664-qbafkxtr 24 28 pig pig NN cord-275664-qbafkxtr 24 29 production production NN cord-275664-qbafkxtr 25 1 ( ( -LRB- cord-275664-qbafkxtr 25 2 Allan Allan NNP cord-275664-qbafkxtr 25 3 et et NNP cord-275664-qbafkxtr 25 4 al al NNP cord-275664-qbafkxtr 25 5 . . NNP cord-275664-qbafkxtr 25 6 , , , cord-275664-qbafkxtr 25 7 1998 1998 CD cord-275664-qbafkxtr 25 8 ; ; : cord-275664-qbafkxtr 26 1 Allan Allan NNP cord-275664-qbafkxtr 26 2 and and CC cord-275664-qbafkxtr 26 3 Ellis Ellis NNP cord-275664-qbafkxtr 26 4 , , , cord-275664-qbafkxtr 26 5 2000 2000 CD cord-275664-qbafkxtr 26 6 ; ; : cord-275664-qbafkxtr 26 7 Choi Choi NNP cord-275664-qbafkxtr 26 8 et et FW cord-275664-qbafkxtr 26 9 al al NNP cord-275664-qbafkxtr 26 10 . . NNP cord-275664-qbafkxtr 26 11 , , , cord-275664-qbafkxtr 26 12 2000 2000 CD cord-275664-qbafkxtr 26 13 ; ; : cord-275664-qbafkxtr 26 14 Edwards Edwards NNP cord-275664-qbafkxtr 26 15 and and CC cord-275664-qbafkxtr 26 16 Sands Sands NNPS cord-275664-qbafkxtr 26 17 , , , cord-275664-qbafkxtr 26 18 1994 1994 CD cord-275664-qbafkxtr 26 19 ; ; : cord-275664-qbafkxtr 26 20 Fenaux Fenaux NNP cord-275664-qbafkxtr 26 21 et et NNP cord-275664-qbafkxtr 26 22 al al NNP cord-275664-qbafkxtr 26 23 . . NNP cord-275664-qbafkxtr 26 24 , , , cord-275664-qbafkxtr 26 25 2000 2000 CD cord-275664-qbafkxtr 26 26 ; ; : cord-275664-qbafkxtr 26 27 Mankertz Mankertz NNP cord-275664-qbafkxtr 26 28 et et NNP cord-275664-qbafkxtr 26 29 al al NNP cord-275664-qbafkxtr 26 30 . . NNP cord-275664-qbafkxtr 26 31 , , , cord-275664-qbafkxtr 26 32 2000 2000 CD cord-275664-qbafkxtr 27 1 ; ; : cord-275664-qbafkxtr 27 2 Onuki Onuki NNP cord-275664-qbafkxtr 27 3 et et NNP cord-275664-qbafkxtr 27 4 al al NNP cord-275664-qbafkxtr 27 5 . . . cord-275664-qbafkxtr 28 1 , , , cord-275664-qbafkxtr 28 2 1999 1999 CD cord-275664-qbafkxtr 28 3 ; ; : cord-275664-qbafkxtr 28 4 Segalés Segalés NNP cord-275664-qbafkxtr 28 5 and and CC cord-275664-qbafkxtr 28 6 Domingo Domingo NNP cord-275664-qbafkxtr 28 7 , , , cord-275664-qbafkxtr 28 8 2002 2002 CD cord-275664-qbafkxtr 28 9 ) ) -RRB- cord-275664-qbafkxtr 28 10 . . . cord-275664-qbafkxtr 29 1 Usually usually RB cord-275664-qbafkxtr 29 2 PMWS PMWS NNP cord-275664-qbafkxtr 29 3 appears appear VBZ cord-275664-qbafkxtr 29 4 in in IN cord-275664-qbafkxtr 29 5 pigs pig NNS cord-275664-qbafkxtr 29 6 aged age VBN cord-275664-qbafkxtr 29 7 5 5 CD cord-275664-qbafkxtr 29 8 to to TO cord-275664-qbafkxtr 29 9 18 18 CD cord-275664-qbafkxtr 29 10 weeks week NNS cord-275664-qbafkxtr 29 11 , , , cord-275664-qbafkxtr 29 12 and and CC cord-275664-qbafkxtr 29 13 affected affect VBN cord-275664-qbafkxtr 29 14 pigs pig NNS cord-275664-qbafkxtr 29 15 show show VBP cord-275664-qbafkxtr 29 16 fever fever NN cord-275664-qbafkxtr 29 17 , , , cord-275664-qbafkxtr 29 18 wasting waste VBG cord-275664-qbafkxtr 29 19 or or CC cord-275664-qbafkxtr 29 20 unthriftiness unthriftiness NN cord-275664-qbafkxtr 29 21 , , , cord-275664-qbafkxtr 29 22 respiratory respiratory JJ cord-275664-qbafkxtr 29 23 distress distress NN cord-275664-qbafkxtr 29 24 , , , cord-275664-qbafkxtr 29 25 enlarged enlarged JJ cord-275664-qbafkxtr 29 26 lymph lymph NN cord-275664-qbafkxtr 29 27 nodes node NNS cord-275664-qbafkxtr 29 28 and and CC cord-275664-qbafkxtr 29 29 , , , cord-275664-qbafkxtr 29 30 occasionally occasionally RB cord-275664-qbafkxtr 29 31 , , , cord-275664-qbafkxtr 29 32 jaundice jaundice NN cord-275664-qbafkxtr 29 33 and and CC cord-275664-qbafkxtr 29 34 diarrhea diarrhea NN cord-275664-qbafkxtr 29 35 Harding Harding NNP cord-275664-qbafkxtr 29 36 , , , cord-275664-qbafkxtr 29 37 1996 1996 CD cord-275664-qbafkxtr 29 38 ; ; : cord-275664-qbafkxtr 29 39 Segalés Segalés NNP cord-275664-qbafkxtr 29 40 and and CC cord-275664-qbafkxtr 29 41 Domingo Domingo NNP cord-275664-qbafkxtr 29 42 , , , cord-275664-qbafkxtr 29 43 2002 2002 CD cord-275664-qbafkxtr 29 44 ) ) -RRB- cord-275664-qbafkxtr 29 45 . . . cord-275664-qbafkxtr 30 1 Mortality mortality NN cord-275664-qbafkxtr 30 2 rates rate NNS cord-275664-qbafkxtr 30 3 may may MD cord-275664-qbafkxtr 30 4 vary vary VB cord-275664-qbafkxtr 30 5 from from IN cord-275664-qbafkxtr 30 6 1 1 CD cord-275664-qbafkxtr 30 7 to to IN cord-275664-qbafkxtr 30 8 2 2 CD cord-275664-qbafkxtr 30 9 % % NN cord-275664-qbafkxtr 30 10 up up IN cord-275664-qbafkxtr 30 11 to to TO cord-275664-qbafkxtr 30 12 30 30 CD cord-275664-qbafkxtr 30 13 % % NN cord-275664-qbafkxtr 30 14 in in IN cord-275664-qbafkxtr 30 15 complicated complicated JJ cord-275664-qbafkxtr 30 16 cases case NNS cord-275664-qbafkxtr 30 17 when when WRB cord-275664-qbafkxtr 30 18 co co NNS cord-275664-qbafkxtr 30 19 - - NNS cord-275664-qbafkxtr 30 20 infections infection NNS cord-275664-qbafkxtr 30 21 with with IN cord-275664-qbafkxtr 30 22 porcine porcine JJ cord-275664-qbafkxtr 30 23 reproductive reproductive JJ cord-275664-qbafkxtr 30 24 and and CC cord-275664-qbafkxtr 30 25 respiratory respiratory JJ cord-275664-qbafkxtr 30 26 syndrome syndrome NN cord-275664-qbafkxtr 30 27 virus virus NN cord-275664-qbafkxtr 30 28 , , , cord-275664-qbafkxtr 30 29 porcine porcine JJ cord-275664-qbafkxtr 30 30 parvovirus parvovirus NN cord-275664-qbafkxtr 30 31 , , , cord-275664-qbafkxtr 30 32 or or CC cord-275664-qbafkxtr 30 33 Mycoplasma Mycoplasma NNP cord-275664-qbafkxtr 30 34 hyopneumoniae hyopneumoniae NN cord-275664-qbafkxtr 30 35 . . . cord-275664-qbafkxtr 31 1 Other other JJ cord-275664-qbafkxtr 31 2 risk risk NN cord-275664-qbafkxtr 31 3 factors factor NNS cord-275664-qbafkxtr 31 4 described describe VBN cord-275664-qbafkxtr 31 5 in in IN cord-275664-qbafkxtr 31 6 the the DT cord-275664-qbafkxtr 31 7 Madec Madec NNP cord-275664-qbafkxtr 31 8 principles principle NNS cord-275664-qbafkxtr 31 9 have have VBP cord-275664-qbafkxtr 31 10 been be VBN cord-275664-qbafkxtr 31 11 developed develop VBN cord-275664-qbafkxtr 31 12 to to TO cord-275664-qbafkxtr 31 13 correlate correlate VB cord-275664-qbafkxtr 31 14 with with IN cord-275664-qbafkxtr 31 15 PMWS PMWS NNP cord-275664-qbafkxtr 31 16 ( ( -LRB- cord-275664-qbafkxtr 31 17 Rose Rose NNP cord-275664-qbafkxtr 31 18 et et FW cord-275664-qbafkxtr 31 19 al al NNP cord-275664-qbafkxtr 31 20 . . NNP cord-275664-qbafkxtr 31 21 , , , cord-275664-qbafkxtr 31 22 2003 2003 CD cord-275664-qbafkxtr 31 23 ) ) -RRB- cord-275664-qbafkxtr 31 24 . . . cord-275664-qbafkxtr 32 1 Microscopic microscopic JJ cord-275664-qbafkxtr 32 2 lesions lesion NNS cord-275664-qbafkxtr 32 3 are be VBP cord-275664-qbafkxtr 32 4 characterized characterize VBN cord-275664-qbafkxtr 32 5 by by IN cord-275664-qbafkxtr 32 6 lymphocyte lymphocyte NN cord-275664-qbafkxtr 32 7 depletion depletion NN cord-275664-qbafkxtr 32 8 of of IN cord-275664-qbafkxtr 32 9 follicular follicular JJ cord-275664-qbafkxtr 32 10 and and CC cord-275664-qbafkxtr 32 11 interfollicular interfollicular JJ cord-275664-qbafkxtr 32 12 areas area NNS cord-275664-qbafkxtr 32 13 together together RB cord-275664-qbafkxtr 32 14 with with IN cord-275664-qbafkxtr 32 15 macrophage macrophage NN cord-275664-qbafkxtr 32 16 infiltration infiltration NN cord-275664-qbafkxtr 32 17 of of IN cord-275664-qbafkxtr 32 18 lymphoid lymphoid JJ cord-275664-qbafkxtr 32 19 tissues tissue NNS cord-275664-qbafkxtr 32 20 in in IN cord-275664-qbafkxtr 32 21 PMWS PMWS NNP cord-275664-qbafkxtr 32 22 - - HYPH cord-275664-qbafkxtr 32 23 affected affect VBN cord-275664-qbafkxtr 32 24 pigs pig NNS cord-275664-qbafkxtr 32 25 . . . cord-275664-qbafkxtr 33 1 Several several JJ cord-275664-qbafkxtr 33 2 lines line NNS cord-275664-qbafkxtr 33 3 of of IN cord-275664-qbafkxtr 33 4 field field NN cord-275664-qbafkxtr 33 5 and and CC cord-275664-qbafkxtr 33 6 experimental experimental JJ cord-275664-qbafkxtr 33 7 evidence evidence NN cord-275664-qbafkxtr 33 8 have have VBP cord-275664-qbafkxtr 33 9 suggested suggest VBN cord-275664-qbafkxtr 33 10 that that IN cord-275664-qbafkxtr 33 11 severely severely RB cord-275664-qbafkxtr 33 12 PMWS PMWS NNP cord-275664-qbafkxtr 33 13 affected affect VBN cord-275664-qbafkxtr 33 14 pigs pig NNS cord-275664-qbafkxtr 33 15 may may MD cord-275664-qbafkxtr 33 16 develop develop VB cord-275664-qbafkxtr 33 17 immunosuppression immunosuppression NN cord-275664-qbafkxtr 33 18 ( ( -LRB- cord-275664-qbafkxtr 33 19 Segalés Segalés NNP cord-275664-qbafkxtr 33 20 et et NNP cord-275664-qbafkxtr 33 21 al al NNP cord-275664-qbafkxtr 33 22 . . NNP cord-275664-qbafkxtr 33 23 , , , cord-275664-qbafkxtr 33 24 2004 2004 CD cord-275664-qbafkxtr 33 25 ) ) -RRB- cord-275664-qbafkxtr 33 26 . . . cord-275664-qbafkxtr 34 1 PCV PCV NNP cord-275664-qbafkxtr 34 2 genome genome NN cord-275664-qbafkxtr 34 3 is be VBZ cord-275664-qbafkxtr 34 4 a a DT cord-275664-qbafkxtr 34 5 closed closed JJ cord-275664-qbafkxtr 34 6 circular circular JJ cord-275664-qbafkxtr 34 7 , , , cord-275664-qbafkxtr 34 8 single single JJ cord-275664-qbafkxtr 34 9 - - HYPH cord-275664-qbafkxtr 34 10 stranded strand VBN cord-275664-qbafkxtr 34 11 DNA dna NN cord-275664-qbafkxtr 34 12 molecule molecule NN cord-275664-qbafkxtr 34 13 of of IN cord-275664-qbafkxtr 34 14 about about RB cord-275664-qbafkxtr 34 15 1.7 1.7 CD cord-275664-qbafkxtr 34 16 kb kb NNS cord-275664-qbafkxtr 34 17 . . . cord-275664-qbafkxtr 35 1 Two two CD cord-275664-qbafkxtr 35 2 major major JJ cord-275664-qbafkxtr 35 3 open open JJ cord-275664-qbafkxtr 35 4 reading reading NN cord-275664-qbafkxtr 35 5 frames frame NNS cord-275664-qbafkxtr 35 6 ( ( -LRB- cord-275664-qbafkxtr 35 7 ORFs ORFs NNP cord-275664-qbafkxtr 35 8 ) ) -RRB- cord-275664-qbafkxtr 35 9 have have VBP cord-275664-qbafkxtr 35 10 been be VBN cord-275664-qbafkxtr 35 11 recognized recognize VBN cord-275664-qbafkxtr 35 12 for for IN cord-275664-qbafkxtr 35 13 PCV PCV NNP cord-275664-qbafkxtr 35 14 , , , cord-275664-qbafkxtr 35 15 ORF1 ORF1 NNP cord-275664-qbafkxtr 35 16 , , , cord-275664-qbafkxtr 35 17 called call VBD cord-275664-qbafkxtr 35 18 rep rep NNP cord-275664-qbafkxtr 35 19 gene gene NN cord-275664-qbafkxtr 35 20 , , , cord-275664-qbafkxtr 35 21 which which WDT cord-275664-qbafkxtr 35 22 encodes encode VBZ cord-275664-qbafkxtr 35 23 a a DT cord-275664-qbafkxtr 35 24 protein protein NN cord-275664-qbafkxtr 35 25 of of IN cord-275664-qbafkxtr 35 26 35.7 35.7 CD cord-275664-qbafkxtr 35 27 kDa kDa NNS cord-275664-qbafkxtr 35 28 involved involve VBN cord-275664-qbafkxtr 35 29 in in IN cord-275664-qbafkxtr 35 30 virus virus NN cord-275664-qbafkxtr 35 31 replication replication NN cord-275664-qbafkxtr 35 32 ( ( -LRB- cord-275664-qbafkxtr 36 1 Mankertz Mankertz NNP cord-275664-qbafkxtr 36 2 et et NNP cord-275664-qbafkxtr 36 3 al al NNP cord-275664-qbafkxtr 36 4 . . NNP cord-275664-qbafkxtr 36 5 , , , cord-275664-qbafkxtr 36 6 1998 1998 CD cord-275664-qbafkxtr 36 7 ) ) -RRB- cord-275664-qbafkxtr 36 8 , , , cord-275664-qbafkxtr 36 9 and and CC cord-275664-qbafkxtr 36 10 ORF2 ORF2 NNP cord-275664-qbafkxtr 36 11 , , , cord-275664-qbafkxtr 36 12 called call VBN cord-275664-qbafkxtr 36 13 cap cap NN cord-275664-qbafkxtr 36 14 gene gene NN cord-275664-qbafkxtr 36 15 , , , cord-275664-qbafkxtr 36 16 which which WDT cord-275664-qbafkxtr 36 17 encodes encode VBZ cord-275664-qbafkxtr 36 18 the the DT cord-275664-qbafkxtr 36 19 major major JJ cord-275664-qbafkxtr 36 20 immunogenic immunogenic JJ cord-275664-qbafkxtr 36 21 capsid capsid NN cord-275664-qbafkxtr 36 22 protein protein NN cord-275664-qbafkxtr 36 23 of of IN cord-275664-qbafkxtr 36 24 27.8 27.8 CD cord-275664-qbafkxtr 36 25 kDa kDa NNPS cord-275664-qbafkxtr 36 26 ( ( -LRB- cord-275664-qbafkxtr 36 27 Cheung Cheung NNP cord-275664-qbafkxtr 36 28 , , , cord-275664-qbafkxtr 36 29 2003 2003 CD cord-275664-qbafkxtr 36 30 ; ; : cord-275664-qbafkxtr 36 31 Nawagitgul Nawagitgul NNP cord-275664-qbafkxtr 36 32 et et NNP cord-275664-qbafkxtr 36 33 al al NNP cord-275664-qbafkxtr 36 34 . . NNP cord-275664-qbafkxtr 36 35 , , , cord-275664-qbafkxtr 36 36 2000 2000 CD cord-275664-qbafkxtr 36 37 ) ) -RRB- cord-275664-qbafkxtr 36 38 . . . cord-275664-qbafkxtr 37 1 In in IN cord-275664-qbafkxtr 37 2 addition addition NN cord-275664-qbafkxtr 37 3 , , , cord-275664-qbafkxtr 37 4 a a DT cord-275664-qbafkxtr 37 5 third third JJ cord-275664-qbafkxtr 37 6 open open JJ cord-275664-qbafkxtr 37 7 reading reading NN cord-275664-qbafkxtr 37 8 frame frame NN cord-275664-qbafkxtr 37 9 ( ( -LRB- cord-275664-qbafkxtr 37 10 ORF3 orf3 NN cord-275664-qbafkxtr 37 11 ) ) -RRB- cord-275664-qbafkxtr 37 12 coding code VBG cord-275664-qbafkxtr 37 13 for for IN cord-275664-qbafkxtr 37 14 an an DT cord-275664-qbafkxtr 37 15 apoptosisassociated apoptosisassociated JJ cord-275664-qbafkxtr 37 16 protein protein NN cord-275664-qbafkxtr 37 17 has have VBZ cord-275664-qbafkxtr 37 18 been be VBN cord-275664-qbafkxtr 37 19 reported report VBN cord-275664-qbafkxtr 37 20 for for IN cord-275664-qbafkxtr 37 21 PCV2 PCV2 NNP cord-275664-qbafkxtr 38 1 and and CC cord-275664-qbafkxtr 38 2 it -PRON- PRP cord-275664-qbafkxtr 38 3 is be VBZ cord-275664-qbafkxtr 38 4 involved involve VBN cord-275664-qbafkxtr 38 5 in in IN cord-275664-qbafkxtr 38 6 viral viral JJ cord-275664-qbafkxtr 38 7 pathogenesis pathogenesis NN cord-275664-qbafkxtr 38 8 in in IN cord-275664-qbafkxtr 38 9 vitro vitro FW cord-275664-qbafkxtr 38 10 and and CC cord-275664-qbafkxtr 38 11 in in IN cord-275664-qbafkxtr 38 12 vivo vivo NN cord-275664-qbafkxtr 39 1 ( ( -LRB- cord-275664-qbafkxtr 39 2 Liu Liu NNP cord-275664-qbafkxtr 39 3 et et NNP cord-275664-qbafkxtr 39 4 al al NNP cord-275664-qbafkxtr 39 5 . . . cord-275664-qbafkxtr 40 1 , , , cord-275664-qbafkxtr 40 2 2005 2005 CD cord-275664-qbafkxtr 41 1 ( ( -LRB- cord-275664-qbafkxtr 41 2 Liu Liu NNP cord-275664-qbafkxtr 41 3 et et NNP cord-275664-qbafkxtr 41 4 al al NNP cord-275664-qbafkxtr 41 5 . . NNP cord-275664-qbafkxtr 41 6 , , , cord-275664-qbafkxtr 41 7 , , , cord-275664-qbafkxtr 41 8 2006 2006 CD cord-275664-qbafkxtr 41 9 . . . cord-275664-qbafkxtr 42 1 A a DT cord-275664-qbafkxtr 42 2 recent recent JJ cord-275664-qbafkxtr 42 3 report report NN cord-275664-qbafkxtr 42 4 has have VBZ cord-275664-qbafkxtr 42 5 shown show VBN cord-275664-qbafkxtr 42 6 that that IN cord-275664-qbafkxtr 42 7 PCV2 PCV2 NNP cord-275664-qbafkxtr 42 8 induces induce VBZ cord-275664-qbafkxtr 42 9 nuclear nuclear JJ cord-275664-qbafkxtr 42 10 factor factor NN cord-275664-qbafkxtr 42 11 kappa kappa NN cord-275664-qbafkxtr 42 12 B B NNP cord-275664-qbafkxtr 42 13 ( ( -LRB- cord-275664-qbafkxtr 42 14 NF NF NNP cord-275664-qbafkxtr 42 15 - - HYPH cord-275664-qbafkxtr 42 16 κB κB NNP cord-275664-qbafkxtr 42 17 ) ) -RRB- cord-275664-qbafkxtr 42 18 activation activation NN cord-275664-qbafkxtr 42 19 in in IN cord-275664-qbafkxtr 42 20 cultured cultured JJ cord-275664-qbafkxtr 42 21 cells cell NNS cord-275664-qbafkxtr 42 22 , , , cord-275664-qbafkxtr 42 23 and and CC cord-275664-qbafkxtr 42 24 further further RB cord-275664-qbafkxtr 42 25 revealed reveal VBD cord-275664-qbafkxtr 42 26 the the DT cord-275664-qbafkxtr 42 27 role role NN cord-275664-qbafkxtr 42 28 of of IN cord-275664-qbafkxtr 42 29 NF nf NN cord-275664-qbafkxtr 42 30 - - HYPH cord-275664-qbafkxtr 42 31 κB κb CD cord-275664-qbafkxtr 42 32 activation activation NN cord-275664-qbafkxtr 42 33 in in IN cord-275664-qbafkxtr 42 34 viral viral JJ cord-275664-qbafkxtr 42 35 replication replication NN cord-275664-qbafkxtr 42 36 and and CC cord-275664-qbafkxtr 42 37 PCV2-mediated pcv2-mediated JJ cord-275664-qbafkxtr 42 38 apoptotic apoptotic JJ cord-275664-qbafkxtr 42 39 change change NN cord-275664-qbafkxtr 42 40 ( ( -LRB- cord-275664-qbafkxtr 42 41 Wei Wei NNP cord-275664-qbafkxtr 42 42 et et NNP cord-275664-qbafkxtr 42 43 al al NNP cord-275664-qbafkxtr 42 44 . . NNP cord-275664-qbafkxtr 42 45 , , , cord-275664-qbafkxtr 42 46 2008 2008 CD cord-275664-qbafkxtr 42 47 ) ) -RRB- cord-275664-qbafkxtr 42 48 . . . cord-275664-qbafkxtr 43 1 However however RB cord-275664-qbafkxtr 43 2 , , , cord-275664-qbafkxtr 43 3 whether whether IN cord-275664-qbafkxtr 43 4 the the DT cord-275664-qbafkxtr 43 5 other other JJ cord-275664-qbafkxtr 43 6 signaling signal VBG cord-275664-qbafkxtr 43 7 pathways pathway NNS cord-275664-qbafkxtr 43 8 may may MD cord-275664-qbafkxtr 43 9 also also RB cord-275664-qbafkxtr 43 10 contribute contribute VB cord-275664-qbafkxtr 43 11 to to IN cord-275664-qbafkxtr 43 12 PCV2 pcv2 JJ cord-275664-qbafkxtr 43 13 infection infection NN cord-275664-qbafkxtr 43 14 in in IN cord-275664-qbafkxtr 43 15 the the DT cord-275664-qbafkxtr 43 16 cultured cultured JJ cord-275664-qbafkxtr 43 17 cells cell NNS cord-275664-qbafkxtr 43 18 is be VBZ cord-275664-qbafkxtr 43 19 still still RB cord-275664-qbafkxtr 43 20 unclear unclear JJ cord-275664-qbafkxtr 43 21 . . . cord-275664-qbafkxtr 44 1 The the DT cord-275664-qbafkxtr 44 2 extracellular extracellular JJ cord-275664-qbafkxtr 44 3 signal signal NN cord-275664-qbafkxtr 44 4 - - HYPH cord-275664-qbafkxtr 44 5 regulated regulate VBN cord-275664-qbafkxtr 44 6 kinase kinase NN cord-275664-qbafkxtr 44 7 ( ( -LRB- cord-275664-qbafkxtr 44 8 ERK ERK NNP cord-275664-qbafkxtr 44 9 ) ) -RRB- cord-275664-qbafkxtr 44 10 signaling signal VBG cord-275664-qbafkxtr 44 11 pathway pathway NN cord-275664-qbafkxtr 44 12 is be VBZ cord-275664-qbafkxtr 44 13 one one CD cord-275664-qbafkxtr 44 14 of of IN cord-275664-qbafkxtr 44 15 the the DT cord-275664-qbafkxtr 44 16 three three CD cord-275664-qbafkxtr 44 17 mitogen mitogen NN cord-275664-qbafkxtr 44 18 - - HYPH cord-275664-qbafkxtr 44 19 activated activate VBN cord-275664-qbafkxtr 44 20 protein protein NN cord-275664-qbafkxtr 44 21 kinase kinase NN cord-275664-qbafkxtr 44 22 ( ( -LRB- cord-275664-qbafkxtr 44 23 MAPK MAPK NNP cord-275664-qbafkxtr 44 24 ) ) -RRB- cord-275664-qbafkxtr 44 25 cascades cascade NNS cord-275664-qbafkxtr 44 26 that that WDT cord-275664-qbafkxtr 44 27 play play VBP cord-275664-qbafkxtr 44 28 important important JJ cord-275664-qbafkxtr 44 29 roles role NNS cord-275664-qbafkxtr 44 30 in in IN cord-275664-qbafkxtr 44 31 the the DT cord-275664-qbafkxtr 44 32 regulation regulation NN cord-275664-qbafkxtr 44 33 of of IN cord-275664-qbafkxtr 44 34 cell cell NN cord-275664-qbafkxtr 44 35 proliferation proliferation NN cord-275664-qbafkxtr 44 36 and and CC cord-275664-qbafkxtr 44 37 Virology Virology NNP cord-275664-qbafkxtr 44 38 386 386 CD cord-275664-qbafkxtr 44 39 ( ( -LRB- cord-275664-qbafkxtr 44 40 2009 2009 CD cord-275664-qbafkxtr 44 41 ) ) -RRB- cord-275664-qbafkxtr 44 42 203 203 CD cord-275664-qbafkxtr 44 43 - - SYM cord-275664-qbafkxtr 44 44 209 209 CD cord-275664-qbafkxtr 44 45 differentiation differentiation NN cord-275664-qbafkxtr 44 46 , , , cord-275664-qbafkxtr 44 47 cytokine cytokine NN cord-275664-qbafkxtr 44 48 production production NN cord-275664-qbafkxtr 44 49 and and CC cord-275664-qbafkxtr 44 50 apoptosis apoptosis NN cord-275664-qbafkxtr 44 51 ( ( -LRB- cord-275664-qbafkxtr 44 52 Garrington Garrington NNP cord-275664-qbafkxtr 44 53 and and CC cord-275664-qbafkxtr 44 54 Johnson Johnson NNP cord-275664-qbafkxtr 44 55 , , , cord-275664-qbafkxtr 44 56 1999 1999 CD cord-275664-qbafkxtr 44 57 ; ; : cord-275664-qbafkxtr 44 58 Roux Roux NNP cord-275664-qbafkxtr 44 59 and and CC cord-275664-qbafkxtr 44 60 Blenis Blenis NNP cord-275664-qbafkxtr 44 61 , , , cord-275664-qbafkxtr 44 62 2004 2004 CD cord-275664-qbafkxtr 44 63 ) ) -RRB- cord-275664-qbafkxtr 44 64 . . . cord-275664-qbafkxtr 45 1 The the DT cord-275664-qbafkxtr 45 2 ERK ERK NNP cord-275664-qbafkxtr 45 3 activation activation NN cord-275664-qbafkxtr 45 4 is be VBZ cord-275664-qbafkxtr 45 5 initiated initiate VBN cord-275664-qbafkxtr 45 6 by by IN cord-275664-qbafkxtr 45 7 receptor receptor NN cord-275664-qbafkxtr 45 8 tyrosine tyrosine NN cord-275664-qbafkxtr 45 9 kinases kinase NNS cord-275664-qbafkxtr 45 10 that that WDT cord-275664-qbafkxtr 45 11 signal signal VBP cord-275664-qbafkxtr 45 12 through through IN cord-275664-qbafkxtr 45 13 the the DT cord-275664-qbafkxtr 45 14 small small JJ cord-275664-qbafkxtr 45 15 GTPbinding gtpbinding NN cord-275664-qbafkxtr 45 16 protein protein NN cord-275664-qbafkxtr 45 17 Ras Ras NNP cord-275664-qbafkxtr 45 18 . . . cord-275664-qbafkxtr 46 1 Activation activation NN cord-275664-qbafkxtr 46 2 of of IN cord-275664-qbafkxtr 46 3 Ras Ras NNP cord-275664-qbafkxtr 46 4 leads lead VBZ cord-275664-qbafkxtr 46 5 to to IN cord-275664-qbafkxtr 46 6 phosphorylation phosphorylation NN cord-275664-qbafkxtr 46 7 of of IN cord-275664-qbafkxtr 46 8 Raf Raf NNP cord-275664-qbafkxtr 46 9 kinase kinase NN cord-275664-qbafkxtr 46 10 and and CC cord-275664-qbafkxtr 46 11 in in IN cord-275664-qbafkxtr 46 12 turn turn NN cord-275664-qbafkxtr 46 13 phosphorylates phosphorylate VBZ cord-275664-qbafkxtr 46 14 MEK1/2 mek1/2 XX cord-275664-qbafkxtr 47 1 followed follow VBN cord-275664-qbafkxtr 47 2 by by IN cord-275664-qbafkxtr 47 3 activating activate VBG cord-275664-qbafkxtr 47 4 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 47 5 via via IN cord-275664-qbafkxtr 47 6 phosphorylation phosphorylation NN cord-275664-qbafkxtr 47 7 on on IN cord-275664-qbafkxtr 47 8 tyrosine tyrosine NN cord-275664-qbafkxtr 47 9 and and CC cord-275664-qbafkxtr 47 10 threonine threonine NN cord-275664-qbafkxtr 47 11 residues residue NNS cord-275664-qbafkxtr 47 12 ( ( -LRB- cord-275664-qbafkxtr 47 13 Rubinfeld Rubinfeld NNP cord-275664-qbafkxtr 47 14 and and CC cord-275664-qbafkxtr 47 15 Seger Seger NNP cord-275664-qbafkxtr 47 16 , , , cord-275664-qbafkxtr 47 17 2005 2005 CD cord-275664-qbafkxtr 47 18 ) ) -RRB- cord-275664-qbafkxtr 47 19 . . . cord-275664-qbafkxtr 48 1 The the DT cord-275664-qbafkxtr 48 2 activated activate VBN cord-275664-qbafkxtr 48 3 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 48 4 translocates translocate NNS cord-275664-qbafkxtr 48 5 into into IN cord-275664-qbafkxtr 48 6 the the DT cord-275664-qbafkxtr 48 7 nucleus nucleus NN cord-275664-qbafkxtr 48 8 and and CC cord-275664-qbafkxtr 48 9 phosphorylates phosphorylate VBZ cord-275664-qbafkxtr 48 10 numerous numerous JJ cord-275664-qbafkxtr 48 11 downstream downstream JJ cord-275664-qbafkxtr 48 12 substrates substrate NNS cord-275664-qbafkxtr 48 13 such such JJ cord-275664-qbafkxtr 48 14 as as IN cord-275664-qbafkxtr 48 15 transcription transcription NN cord-275664-qbafkxtr 48 16 factors factor NNS cord-275664-qbafkxtr 48 17 c c NNP cord-275664-qbafkxtr 48 18 - - HYPH cord-275664-qbafkxtr 48 19 myc myc NNP cord-275664-qbafkxtr 48 20 , , , cord-275664-qbafkxtr 48 21 Ets Ets NNP cord-275664-qbafkxtr 48 22 , , , cord-275664-qbafkxtr 48 23 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 48 24 , , , cord-275664-qbafkxtr 48 25 and and CC cord-275664-qbafkxtr 48 26 Egr-1 Egr-1 NNP cord-275664-qbafkxtr 48 27 , , , cord-275664-qbafkxtr 48 28 which which WDT cord-275664-qbafkxtr 48 29 ultimately ultimately RB cord-275664-qbafkxtr 48 30 regulate regulate VBP cord-275664-qbafkxtr 48 31 gene gene NN cord-275664-qbafkxtr 48 32 expression expression NN cord-275664-qbafkxtr 48 33 ( ( -LRB- cord-275664-qbafkxtr 48 34 Luttrell Luttrell NNP cord-275664-qbafkxtr 48 35 , , , cord-275664-qbafkxtr 48 36 2003 2003 CD cord-275664-qbafkxtr 48 37 ) ) -RRB- cord-275664-qbafkxtr 48 38 . . . cord-275664-qbafkxtr 49 1 Therefore therefore RB cord-275664-qbafkxtr 49 2 , , , cord-275664-qbafkxtr 49 3 the the DT cord-275664-qbafkxtr 49 4 ERK ERK NNP cord-275664-qbafkxtr 49 5 signaling signal VBG cord-275664-qbafkxtr 49 6 pathway pathway NN cord-275664-qbafkxtr 49 7 involves involve VBZ cord-275664-qbafkxtr 49 8 in in IN cord-275664-qbafkxtr 49 9 a a DT cord-275664-qbafkxtr 49 10 wide wide JJ cord-275664-qbafkxtr 49 11 range range NN cord-275664-qbafkxtr 49 12 of of IN cord-275664-qbafkxtr 49 13 cellular cellular JJ cord-275664-qbafkxtr 49 14 functions function NNS cord-275664-qbafkxtr 49 15 including include VBG cord-275664-qbafkxtr 49 16 cell cell NN cord-275664-qbafkxtr 49 17 proliferation proliferation NN cord-275664-qbafkxtr 49 18 , , , cord-275664-qbafkxtr 49 19 transformatiom transformatiom JJ cord-275664-qbafkxtr 49 20 , , , cord-275664-qbafkxtr 49 21 differentiation differentiation NN cord-275664-qbafkxtr 49 22 , , , cord-275664-qbafkxtr 49 23 and and CC cord-275664-qbafkxtr 49 24 cell cell NN cord-275664-qbafkxtr 49 25 survival survival NN cord-275664-qbafkxtr 49 26 and and CC cord-275664-qbafkxtr 49 27 death death NN cord-275664-qbafkxtr 49 28 ( ( -LRB- cord-275664-qbafkxtr 49 29 Sebolt Sebolt NNP cord-275664-qbafkxtr 49 30 - - HYPH cord-275664-qbafkxtr 49 31 Leopold Leopold NNP cord-275664-qbafkxtr 49 32 et et FW cord-275664-qbafkxtr 49 33 al al NNP cord-275664-qbafkxtr 49 34 . . . cord-275664-qbafkxtr 50 1 , , , cord-275664-qbafkxtr 50 2 1999 1999 CD cord-275664-qbafkxtr 50 3 ; ; : cord-275664-qbafkxtr 50 4 Luttrell Luttrell NNP cord-275664-qbafkxtr 50 5 , , , cord-275664-qbafkxtr 50 6 2003 2003 CD cord-275664-qbafkxtr 50 7 ) ) -RRB- cord-275664-qbafkxtr 50 8 . . . cord-275664-qbafkxtr 51 1 Research research NN cord-275664-qbafkxtr 51 2 data datum NNS cord-275664-qbafkxtr 51 3 have have VBP cord-275664-qbafkxtr 51 4 shown show VBN cord-275664-qbafkxtr 51 5 that that IN cord-275664-qbafkxtr 51 6 many many JJ cord-275664-qbafkxtr 51 7 viruses virus NNS cord-275664-qbafkxtr 51 8 such such JJ cord-275664-qbafkxtr 51 9 as as IN cord-275664-qbafkxtr 51 10 human human JJ cord-275664-qbafkxtr 51 11 cytomegalovirus cytomegalovirus NN cord-275664-qbafkxtr 51 12 ( ( -LRB- cord-275664-qbafkxtr 51 13 Johnson Johnson NNP cord-275664-qbafkxtr 51 14 et et NNP cord-275664-qbafkxtr 51 15 al al NNP cord-275664-qbafkxtr 51 16 . . NNP cord-275664-qbafkxtr 51 17 , , , cord-275664-qbafkxtr 51 18 2001 2001 CD cord-275664-qbafkxtr 51 19 ) ) -RRB- cord-275664-qbafkxtr 51 20 , , , cord-275664-qbafkxtr 51 21 human human JJ cord-275664-qbafkxtr 51 22 immunodeficiency immunodeficiency NN cord-275664-qbafkxtr 51 23 virus virus NN cord-275664-qbafkxtr 51 24 type type NN cord-275664-qbafkxtr 51 25 1 1 CD cord-275664-qbafkxtr 52 1 ( ( -LRB- cord-275664-qbafkxtr 52 2 Yang Yang NNP cord-275664-qbafkxtr 52 3 and and CC cord-275664-qbafkxtr 52 4 Gabuzda Gabuzda NNP cord-275664-qbafkxtr 52 5 , , , cord-275664-qbafkxtr 52 6 1999 1999 CD cord-275664-qbafkxtr 52 7 ) ) -RRB- cord-275664-qbafkxtr 52 8 , , , cord-275664-qbafkxtr 52 9 influenza influenza NN cord-275664-qbafkxtr 52 10 virus virus NN cord-275664-qbafkxtr 52 11 , , , cord-275664-qbafkxtr 52 12 herpes herpes NN cord-275664-qbafkxtr 52 13 virus virus NN cord-275664-qbafkxtr 52 14 ( ( -LRB- cord-275664-qbafkxtr 52 15 Perkins Perkins NNP cord-275664-qbafkxtr 52 16 et et NNP cord-275664-qbafkxtr 52 17 al al NNP cord-275664-qbafkxtr 52 18 . . NNP cord-275664-qbafkxtr 52 19 , , , cord-275664-qbafkxtr 52 20 2002 2002 CD cord-275664-qbafkxtr 52 21 ) ) -RRB- cord-275664-qbafkxtr 52 22 , , , cord-275664-qbafkxtr 52 23 coxsackievirus coxsackievirus NNP cord-275664-qbafkxtr 52 24 B3 B3 NNP cord-275664-qbafkxtr 52 25 ( ( -LRB- cord-275664-qbafkxtr 52 26 Luo Luo NNP cord-275664-qbafkxtr 52 27 et et NNP cord-275664-qbafkxtr 52 28 al al NNP cord-275664-qbafkxtr 52 29 . . NNP cord-275664-qbafkxtr 52 30 , , , cord-275664-qbafkxtr 52 31 2002 2002 CD cord-275664-qbafkxtr 52 32 ) ) -RRB- cord-275664-qbafkxtr 52 33 , , , cord-275664-qbafkxtr 52 34 vaccinia vaccinia NN cord-275664-qbafkxtr 52 35 virus virus NN cord-275664-qbafkxtr 52 36 ( ( -LRB- cord-275664-qbafkxtr 52 37 Andrade Andrade NNP cord-275664-qbafkxtr 52 38 et et NNP cord-275664-qbafkxtr 52 39 al al NNP cord-275664-qbafkxtr 52 40 . . NNP cord-275664-qbafkxtr 52 41 , , , cord-275664-qbafkxtr 52 42 2004 2004 CD cord-275664-qbafkxtr 52 43 ; ; : cord-275664-qbafkxtr 52 44 de de NNP cord-275664-qbafkxtr 52 45 Magalhães Magalhães NNP cord-275664-qbafkxtr 52 46 et et NNP cord-275664-qbafkxtr 52 47 al al NNP cord-275664-qbafkxtr 52 48 . . NNP cord-275664-qbafkxtr 52 49 , , , cord-275664-qbafkxtr 52 50 2001 2001 CD cord-275664-qbafkxtr 52 51 ) ) -RRB- cord-275664-qbafkxtr 52 52 , , , cord-275664-qbafkxtr 52 53 respiratory respiratory JJ cord-275664-qbafkxtr 52 54 syncytial syncytial JJ cord-275664-qbafkxtr 52 55 virus virus NN cord-275664-qbafkxtr 52 56 ( ( -LRB- cord-275664-qbafkxtr 52 57 Kong Kong NNP cord-275664-qbafkxtr 52 58 et et NNP cord-275664-qbafkxtr 52 59 al al NNP cord-275664-qbafkxtr 52 60 . . NNP cord-275664-qbafkxtr 52 61 , , , cord-275664-qbafkxtr 52 62 2004 2004 CD cord-275664-qbafkxtr 52 63 ) ) -RRB- cord-275664-qbafkxtr 52 64 , , , cord-275664-qbafkxtr 52 65 Borna Borna NNP cord-275664-qbafkxtr 52 66 disease disease NN cord-275664-qbafkxtr 52 67 virus virus NN cord-275664-qbafkxtr 52 68 , , , cord-275664-qbafkxtr 52 69 and and CC cord-275664-qbafkxtr 52 70 coronavirus coronavirus NN cord-275664-qbafkxtr 52 71 ( ( -LRB- cord-275664-qbafkxtr 52 72 Cai Cai NNP cord-275664-qbafkxtr 52 73 et et FW cord-275664-qbafkxtr 52 74 al al NNP cord-275664-qbafkxtr 52 75 . . NNP cord-275664-qbafkxtr 52 76 , , , cord-275664-qbafkxtr 52 77 2007 2007 CD cord-275664-qbafkxtr 52 78 ) ) -RRB- cord-275664-qbafkxtr 52 79 manipulate manipulate VBP cord-275664-qbafkxtr 52 80 the the DT cord-275664-qbafkxtr 52 81 ERK ERK NNP cord-275664-qbafkxtr 52 82 signaling signal VBG cord-275664-qbafkxtr 52 83 pathway pathway NN cord-275664-qbafkxtr 52 84 to to TO cord-275664-qbafkxtr 52 85 regulate regulate VB cord-275664-qbafkxtr 52 86 viral viral JJ cord-275664-qbafkxtr 52 87 replication replication NN cord-275664-qbafkxtr 52 88 and and CC cord-275664-qbafkxtr 52 89 gene gene NN cord-275664-qbafkxtr 52 90 expression expression NN cord-275664-qbafkxtr 52 91 . . . cord-275664-qbafkxtr 53 1 However however RB cord-275664-qbafkxtr 53 2 , , , cord-275664-qbafkxtr 53 3 the the DT cord-275664-qbafkxtr 53 4 role role NN cord-275664-qbafkxtr 53 5 of of IN cord-275664-qbafkxtr 53 6 the the DT cord-275664-qbafkxtr 53 7 ERK ERK NNP cord-275664-qbafkxtr 53 8 signaling signal VBG cord-275664-qbafkxtr 53 9 pathway pathway NN cord-275664-qbafkxtr 53 10 during during IN cord-275664-qbafkxtr 53 11 PCV2 pcv2 JJ cord-275664-qbafkxtr 53 12 replication replication NN cord-275664-qbafkxtr 53 13 is be VBZ cord-275664-qbafkxtr 53 14 not not RB cord-275664-qbafkxtr 53 15 clear clear JJ cord-275664-qbafkxtr 53 16 . . . cord-275664-qbafkxtr 54 1 Here here RB cord-275664-qbafkxtr 54 2 , , , cord-275664-qbafkxtr 54 3 we -PRON- PRP cord-275664-qbafkxtr 54 4 reported report VBD cord-275664-qbafkxtr 54 5 that that IN cord-275664-qbafkxtr 54 6 the the DT cord-275664-qbafkxtr 54 7 ERK ERK NNP cord-275664-qbafkxtr 54 8 signaling signal VBG cord-275664-qbafkxtr 54 9 pathway pathway NN cord-275664-qbafkxtr 54 10 was be VBD cord-275664-qbafkxtr 54 11 activated activate VBN cord-275664-qbafkxtr 54 12 in in IN cord-275664-qbafkxtr 54 13 the the DT cord-275664-qbafkxtr 54 14 cultured cultured JJ cord-275664-qbafkxtr 54 15 cells cell NNS cord-275664-qbafkxtr 54 16 by by IN cord-275664-qbafkxtr 54 17 PCV2 pcv2 JJ cord-275664-qbafkxtr 54 18 infection infection NN cord-275664-qbafkxtr 54 19 and and CC cord-275664-qbafkxtr 54 20 found find VBD cord-275664-qbafkxtr 54 21 that that DT cord-275664-qbafkxtr 54 22 activation activation NN cord-275664-qbafkxtr 54 23 of of IN cord-275664-qbafkxtr 54 24 the the DT cord-275664-qbafkxtr 54 25 host host NN cord-275664-qbafkxtr 54 26 cellular cellular JJ cord-275664-qbafkxtr 54 27 pathway pathway NN cord-275664-qbafkxtr 54 28 is be VBZ cord-275664-qbafkxtr 54 29 essential essential JJ cord-275664-qbafkxtr 54 30 for for IN cord-275664-qbafkxtr 54 31 efficient efficient JJ cord-275664-qbafkxtr 54 32 PCV2 pcv2 JJ cord-275664-qbafkxtr 54 33 infection infection NN cord-275664-qbafkxtr 54 34 . . . cord-275664-qbafkxtr 55 1 Further further JJ cord-275664-qbafkxtr 55 2 experiments experiment NNS cord-275664-qbafkxtr 55 3 revealed reveal VBD cord-275664-qbafkxtr 55 4 that that IN cord-275664-qbafkxtr 55 5 inhibition inhibition NN cord-275664-qbafkxtr 55 6 of of IN cord-275664-qbafkxtr 55 7 this this DT cord-275664-qbafkxtr 55 8 pathway pathway NN cord-275664-qbafkxtr 55 9 reduces reduce VBZ cord-275664-qbafkxtr 55 10 viral viral JJ cord-275664-qbafkxtr 55 11 transcription transcription NN cord-275664-qbafkxtr 55 12 , , , cord-275664-qbafkxtr 55 13 protein protein NN cord-275664-qbafkxtr 55 14 synthesis synthesis NN cord-275664-qbafkxtr 55 15 , , , cord-275664-qbafkxtr 55 16 and and CC cord-275664-qbafkxtr 55 17 PCV2 pcv2 JJ cord-275664-qbafkxtr 55 18 viral viral JJ cord-275664-qbafkxtr 55 19 progeny progeny NN cord-275664-qbafkxtr 55 20 release release NN cord-275664-qbafkxtr 55 21 . . . cord-275664-qbafkxtr 56 1 These these DT cord-275664-qbafkxtr 56 2 results result NNS cord-275664-qbafkxtr 56 3 illustrate illustrate VBP cord-275664-qbafkxtr 56 4 a a DT cord-275664-qbafkxtr 56 5 mechanism mechanism NN cord-275664-qbafkxtr 56 6 by by IN cord-275664-qbafkxtr 56 7 which which WDT cord-275664-qbafkxtr 56 8 PCV2 PCV2 NNP cord-275664-qbafkxtr 56 9 manipulates manipulate VBZ cord-275664-qbafkxtr 56 10 the the DT cord-275664-qbafkxtr 56 11 ERK ERK NNP cord-275664-qbafkxtr 56 12 signaling signal VBG cord-275664-qbafkxtr 56 13 pathway pathway NN cord-275664-qbafkxtr 56 14 to to TO cord-275664-qbafkxtr 56 15 facilitate facilitate VB cord-275664-qbafkxtr 56 16 its -PRON- PRP$ cord-275664-qbafkxtr 56 17 infection infection NN cord-275664-qbafkxtr 56 18 and and CC cord-275664-qbafkxtr 56 19 replication replication NN cord-275664-qbafkxtr 56 20 during during IN cord-275664-qbafkxtr 56 21 viral viral JJ cord-275664-qbafkxtr 56 22 infection infection NN cord-275664-qbafkxtr 56 23 . . . cord-275664-qbafkxtr 57 1 It -PRON- PRP cord-275664-qbafkxtr 57 2 was be VBD cord-275664-qbafkxtr 57 3 reported report VBN cord-275664-qbafkxtr 57 4 that that IN cord-275664-qbafkxtr 57 5 ERK ERK NNP cord-275664-qbafkxtr 57 6 is be VBZ cord-275664-qbafkxtr 57 7 phosphorylated phosphorylate VBN cord-275664-qbafkxtr 57 8 during during IN cord-275664-qbafkxtr 57 9 varieties variety NNS cord-275664-qbafkxtr 57 10 of of IN cord-275664-qbafkxtr 57 11 virus virus NN cord-275664-qbafkxtr 57 12 replication replication NN cord-275664-qbafkxtr 57 13 . . . cord-275664-qbafkxtr 58 1 In in IN cord-275664-qbafkxtr 58 2 order order NN cord-275664-qbafkxtr 58 3 to to TO cord-275664-qbafkxtr 58 4 assess assess VB cord-275664-qbafkxtr 58 5 whether whether IN cord-275664-qbafkxtr 58 6 PCV2 pcv2 JJ cord-275664-qbafkxtr 58 7 infection infection NN cord-275664-qbafkxtr 58 8 activated activate VBN cord-275664-qbafkxtr 58 9 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 58 10 , , , cord-275664-qbafkxtr 58 11 the the DT cord-275664-qbafkxtr 58 12 phosphorylation phosphorylation NN cord-275664-qbafkxtr 58 13 status status NN cord-275664-qbafkxtr 58 14 of of IN cord-275664-qbafkxtr 58 15 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 58 16 was be VBD cord-275664-qbafkxtr 58 17 monitored monitor VBN cord-275664-qbafkxtr 58 18 on on IN cord-275664-qbafkxtr 58 19 PK15 PK15 NNP cord-275664-qbafkxtr 58 20 cells cell NNS cord-275664-qbafkxtr 58 21 at at IN cord-275664-qbafkxtr 58 22 different different JJ cord-275664-qbafkxtr 58 23 time time NN cord-275664-qbafkxtr 58 24 points point NNS cord-275664-qbafkxtr 58 25 after after IN cord-275664-qbafkxtr 58 26 infection infection NN cord-275664-qbafkxtr 58 27 by by IN cord-275664-qbafkxtr 58 28 Western western JJ cord-275664-qbafkxtr 58 29 blot blot NN cord-275664-qbafkxtr 58 30 analysis analysis NN cord-275664-qbafkxtr 58 31 . . . cord-275664-qbafkxtr 59 1 The the DT cord-275664-qbafkxtr 59 2 PK15 PK15 NNP cord-275664-qbafkxtr 59 3 cells cell NNS cord-275664-qbafkxtr 59 4 were be VBD cord-275664-qbafkxtr 59 5 infected infect VBN cord-275664-qbafkxtr 59 6 with with IN cord-275664-qbafkxtr 59 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 59 8 strain strain NN cord-275664-qbafkxtr 59 9 BJW bjw NN cord-275664-qbafkxtr 59 10 at at IN cord-275664-qbafkxtr 59 11 a a DT cord-275664-qbafkxtr 59 12 MOI moi NN cord-275664-qbafkxtr 59 13 of of IN cord-275664-qbafkxtr 59 14 1 1 CD cord-275664-qbafkxtr 59 15 TCID TCID NNP cord-275664-qbafkxtr 59 16 50 50 CD cord-275664-qbafkxtr 59 17 , , , cord-275664-qbafkxtr 59 18 and and CC cord-275664-qbafkxtr 59 19 whole whole JJ cord-275664-qbafkxtr 59 20 cell cell NN cord-275664-qbafkxtr 59 21 lysates lysate NNS cord-275664-qbafkxtr 59 22 were be VBD cord-275664-qbafkxtr 59 23 prepared prepare VBN cord-275664-qbafkxtr 59 24 at at IN cord-275664-qbafkxtr 59 25 the the DT cord-275664-qbafkxtr 59 26 indicated indicate VBN cord-275664-qbafkxtr 59 27 times time NNS cord-275664-qbafkxtr 59 28 after after IN cord-275664-qbafkxtr 59 29 virus virus NN cord-275664-qbafkxtr 59 30 infection infection NN cord-275664-qbafkxtr 59 31 . . . cord-275664-qbafkxtr 60 1 Inoculation Inoculation NNP cord-275664-qbafkxtr 60 2 of of IN cord-275664-qbafkxtr 60 3 PBS PBS NNP cord-275664-qbafkxtr 60 4 into into IN cord-275664-qbafkxtr 60 5 PK15 PK15 NNP cord-275664-qbafkxtr 60 6 cells cell NNS cord-275664-qbafkxtr 60 7 served serve VBD cord-275664-qbafkxtr 60 8 as as IN cord-275664-qbafkxtr 60 9 mock mock JJ cord-275664-qbafkxtr 60 10 - - HYPH cord-275664-qbafkxtr 60 11 infected infect VBN cord-275664-qbafkxtr 60 12 controls control NNS cord-275664-qbafkxtr 60 13 . . . cord-275664-qbafkxtr 61 1 As as IN cord-275664-qbafkxtr 61 2 shown show VBN cord-275664-qbafkxtr 61 3 in in IN cord-275664-qbafkxtr 61 4 Fig Fig NNP cord-275664-qbafkxtr 61 5 . . . cord-275664-qbafkxtr 62 1 1A 1a LS cord-275664-qbafkxtr 62 2 , , , cord-275664-qbafkxtr 62 3 infection infection NN cord-275664-qbafkxtr 62 4 with with IN cord-275664-qbafkxtr 62 5 PCV2 PCV2 NNP cord-275664-qbafkxtr 62 6 led lead VBD cord-275664-qbafkxtr 62 7 to to IN cord-275664-qbafkxtr 62 8 progressive progressive JJ cord-275664-qbafkxtr 62 9 accumulation accumulation NN cord-275664-qbafkxtr 62 10 of of IN cord-275664-qbafkxtr 62 11 p p NN cord-275664-qbafkxtr 62 12 - - HYPH cord-275664-qbafkxtr 62 13 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 62 14 signals signal NNS cord-275664-qbafkxtr 62 15 over over IN cord-275664-qbafkxtr 62 16 time time NN cord-275664-qbafkxtr 62 17 , , , cord-275664-qbafkxtr 62 18 and and CC cord-275664-qbafkxtr 62 19 the the DT cord-275664-qbafkxtr 62 20 maximal maximal JJ cord-275664-qbafkxtr 62 21 induction induction NN cord-275664-qbafkxtr 62 22 of of IN cord-275664-qbafkxtr 62 23 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 62 24 was be VBD cord-275664-qbafkxtr 62 25 seen see VBN cord-275664-qbafkxtr 62 26 at at IN cord-275664-qbafkxtr 62 27 72 72 CD cord-275664-qbafkxtr 62 28 h h NN cord-275664-qbafkxtr 62 29 postinfection postinfection NN cord-275664-qbafkxtr 62 30 . . . cord-275664-qbafkxtr 63 1 The the DT cord-275664-qbafkxtr 63 2 increased increase VBN cord-275664-qbafkxtr 63 3 levels level NNS cord-275664-qbafkxtr 63 4 of of IN cord-275664-qbafkxtr 63 5 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 63 6 phosphorylation phosphorylation NN cord-275664-qbafkxtr 63 7 were be VBD cord-275664-qbafkxtr 63 8 concurrent concurrent JJ cord-275664-qbafkxtr 63 9 with with IN cord-275664-qbafkxtr 63 10 expression expression NN cord-275664-qbafkxtr 63 11 of of IN cord-275664-qbafkxtr 63 12 viral viral JJ cord-275664-qbafkxtr 63 13 capsid capsid NN cord-275664-qbafkxtr 63 14 protein protein NN cord-275664-qbafkxtr 63 15 ORF2 ORF2 NNP cord-275664-qbafkxtr 63 16 in in IN cord-275664-qbafkxtr 63 17 the the DT cord-275664-qbafkxtr 63 18 infected infected JJ cord-275664-qbafkxtr 63 19 cells cell NNS cord-275664-qbafkxtr 63 20 , , , cord-275664-qbafkxtr 63 21 but but CC cord-275664-qbafkxtr 63 22 not not RB cord-275664-qbafkxtr 63 23 due due IN cord-275664-qbafkxtr 63 24 to to IN cord-275664-qbafkxtr 63 25 the the DT cord-275664-qbafkxtr 63 26 enhanced enhanced JJ cord-275664-qbafkxtr 63 27 production production NN cord-275664-qbafkxtr 63 28 of of IN cord-275664-qbafkxtr 63 29 these these DT cord-275664-qbafkxtr 63 30 molecules molecule NNS cord-275664-qbafkxtr 63 31 or or CC cord-275664-qbafkxtr 63 32 the the DT cord-275664-qbafkxtr 63 33 difference difference NN cord-275664-qbafkxtr 63 34 in in IN cord-275664-qbafkxtr 63 35 protein protein NN cord-275664-qbafkxtr 63 36 extracts extract NNS cord-275664-qbafkxtr 63 37 loaded load VBN cord-275664-qbafkxtr 63 38 , , , cord-275664-qbafkxtr 63 39 as as IN cord-275664-qbafkxtr 63 40 the the DT cord-275664-qbafkxtr 63 41 protein protein NN cord-275664-qbafkxtr 63 42 levels level NNS cord-275664-qbafkxtr 63 43 of of IN cord-275664-qbafkxtr 63 44 total total JJ cord-275664-qbafkxtr 63 45 amounts amount NNS cord-275664-qbafkxtr 63 46 of of IN cord-275664-qbafkxtr 63 47 ERK2 ERK2 NNP cord-275664-qbafkxtr 63 48 , , , cord-275664-qbafkxtr 63 49 as as RB cord-275664-qbafkxtr 63 50 well well RB cord-275664-qbafkxtr 63 51 as as IN cord-275664-qbafkxtr 63 52 β β NN cord-275664-qbafkxtr 63 53 - - HYPH cord-275664-qbafkxtr 63 54 actin actin NN cord-275664-qbafkxtr 63 55 , , , cord-275664-qbafkxtr 63 56 in in IN cord-275664-qbafkxtr 63 57 each each DT cord-275664-qbafkxtr 63 58 sample sample NN cord-275664-qbafkxtr 63 59 were be VBD cord-275664-qbafkxtr 63 60 comparable comparable JJ cord-275664-qbafkxtr 63 61 . . . cord-275664-qbafkxtr 64 1 To to TO cord-275664-qbafkxtr 64 2 further further RB cord-275664-qbafkxtr 64 3 determine determine VB cord-275664-qbafkxtr 64 4 activated activate VBN cord-275664-qbafkxtr 64 5 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 65 1 quantitatively quantitatively RB cord-275664-qbafkxtr 65 2 in in IN cord-275664-qbafkxtr 65 3 the the DT cord-275664-qbafkxtr 65 4 infected infected JJ cord-275664-qbafkxtr 65 5 cells cell NNS cord-275664-qbafkxtr 65 6 , , , cord-275664-qbafkxtr 65 7 we -PRON- PRP cord-275664-qbafkxtr 65 8 used use VBD cord-275664-qbafkxtr 65 9 a a DT cord-275664-qbafkxtr 65 10 Fast Fast NNP cord-275664-qbafkxtr 65 11 Activated Activated NNP cord-275664-qbafkxtr 65 12 Cell cell NN cord-275664-qbafkxtr 65 13 - - HYPH cord-275664-qbafkxtr 65 14 Based base VBN cord-275664-qbafkxtr 65 15 ELISA ELISA NNP cord-275664-qbafkxtr 65 16 ( ( -LRB- cord-275664-qbafkxtr 65 17 FACE face NN cord-275664-qbafkxtr 65 18 ) ) -RRB- cord-275664-qbafkxtr 65 19 assay assay NN cord-275664-qbafkxtr 65 20 to to TO cord-275664-qbafkxtr 65 21 investigate investigate VB cord-275664-qbafkxtr 65 22 the the DT cord-275664-qbafkxtr 65 23 levels level NNS cord-275664-qbafkxtr 65 24 of of IN cord-275664-qbafkxtr 65 25 ability ability NN cord-275664-qbafkxtr 65 26 of of IN cord-275664-qbafkxtr 65 27 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 65 28 phosphorylation phosphorylation NN cord-275664-qbafkxtr 65 29 at at IN cord-275664-qbafkxtr 65 30 different different JJ cord-275664-qbafkxtr 65 31 time time NN cord-275664-qbafkxtr 65 32 points point NNS cord-275664-qbafkxtr 65 33 after after IN cord-275664-qbafkxtr 65 34 PCV2 pcv2 JJ cord-275664-qbafkxtr 65 35 infection infection NN cord-275664-qbafkxtr 65 36 . . . cord-275664-qbafkxtr 66 1 Consistent consistent JJ cord-275664-qbafkxtr 66 2 with with IN cord-275664-qbafkxtr 66 3 the the DT cord-275664-qbafkxtr 66 4 results result NNS cord-275664-qbafkxtr 66 5 shown show VBN cord-275664-qbafkxtr 66 6 in in IN cord-275664-qbafkxtr 66 7 Fig Fig NNP cord-275664-qbafkxtr 66 8 . . . cord-275664-qbafkxtr 67 1 1A 1a LS cord-275664-qbafkxtr 67 2 , , , cord-275664-qbafkxtr 67 3 there there EX cord-275664-qbafkxtr 67 4 was be VBD cord-275664-qbafkxtr 67 5 a a DT cord-275664-qbafkxtr 67 6 time time NN cord-275664-qbafkxtr 67 7 - - HYPH cord-275664-qbafkxtr 67 8 dependent dependent JJ cord-275664-qbafkxtr 67 9 increase increase NN cord-275664-qbafkxtr 67 10 in in IN cord-275664-qbafkxtr 67 11 the the DT cord-275664-qbafkxtr 67 12 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 67 13 phosphorylation phosphorylation NN cord-275664-qbafkxtr 67 14 in in IN cord-275664-qbafkxtr 67 15 the the DT cord-275664-qbafkxtr 67 16 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 67 17 cells cell NNS cord-275664-qbafkxtr 67 18 at at IN cord-275664-qbafkxtr 67 19 72 72 CD cord-275664-qbafkxtr 67 20 h h NN cord-275664-qbafkxtr 67 21 postinfection postinfection NN cord-275664-qbafkxtr 67 22 which which WDT cord-275664-qbafkxtr 67 23 decreased decrease VBD cord-275664-qbafkxtr 67 24 thereafter thereafter RB cord-275664-qbafkxtr 67 25 ( ( -LRB- cord-275664-qbafkxtr 67 26 Fig fig NN cord-275664-qbafkxtr 67 27 . . . cord-275664-qbafkxtr 67 28 1B 1b CD cord-275664-qbafkxtr 67 29 ) ) -RRB- cord-275664-qbafkxtr 67 30 . . . cord-275664-qbafkxtr 68 1 At at IN cord-275664-qbafkxtr 68 2 72 72 CD cord-275664-qbafkxtr 68 3 h h NN cord-275664-qbafkxtr 68 4 after after IN cord-275664-qbafkxtr 68 5 infection infection NN cord-275664-qbafkxtr 68 6 , , , cord-275664-qbafkxtr 68 7 the the DT cord-275664-qbafkxtr 68 8 activation activation NN cord-275664-qbafkxtr 68 9 of of IN cord-275664-qbafkxtr 68 10 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 68 11 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 68 12 showed show VBD cord-275664-qbafkxtr 68 13 approximately approximately RB cord-275664-qbafkxtr 68 14 3.1-fold 3.1-fold CD cord-275664-qbafkxtr 68 15 higher high JJR cord-275664-qbafkxtr 68 16 than than IN cord-275664-qbafkxtr 68 17 that that DT cord-275664-qbafkxtr 68 18 in in IN cord-275664-qbafkxtr 68 19 the the DT cord-275664-qbafkxtr 68 20 mock mock NN cord-275664-qbafkxtr 68 21 - - HYPH cord-275664-qbafkxtr 68 22 infected infect VBN cord-275664-qbafkxtr 68 23 cells cell NNS cord-275664-qbafkxtr 68 24 . . . cord-275664-qbafkxtr 69 1 In in IN cord-275664-qbafkxtr 69 2 the the DT cord-275664-qbafkxtr 69 3 mock mock NN cord-275664-qbafkxtr 69 4 - - HYPH cord-275664-qbafkxtr 69 5 infected infect VBN cord-275664-qbafkxtr 69 6 cells cell NNS cord-275664-qbafkxtr 69 7 , , , cord-275664-qbafkxtr 69 8 the the DT cord-275664-qbafkxtr 69 9 level level NN cord-275664-qbafkxtr 69 10 of of IN cord-275664-qbafkxtr 69 11 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 69 12 phosphorylation phosphorylation NN cord-275664-qbafkxtr 69 13 fell fall VBD cord-275664-qbafkxtr 69 14 to to IN cord-275664-qbafkxtr 69 15 its -PRON- PRP$ cord-275664-qbafkxtr 69 16 basal basal NN cord-275664-qbafkxtr 69 17 level level NN cord-275664-qbafkxtr 69 18 . . . cord-275664-qbafkxtr 70 1 In in IN cord-275664-qbafkxtr 70 2 addition addition NN cord-275664-qbafkxtr 70 3 , , , cord-275664-qbafkxtr 70 4 the the DT cord-275664-qbafkxtr 70 5 levels level NNS cord-275664-qbafkxtr 70 6 of of IN cord-275664-qbafkxtr 70 7 total total JJ cord-275664-qbafkxtr 70 8 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 70 9 remain remain VBP cord-275664-qbafkxtr 70 10 unchanged unchanged JJ cord-275664-qbafkxtr 70 11 in in IN cord-275664-qbafkxtr 70 12 the the DT cord-275664-qbafkxtr 70 13 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 70 14 cells cell NNS cord-275664-qbafkxtr 70 15 at at IN cord-275664-qbafkxtr 70 16 various various JJ cord-275664-qbafkxtr 70 17 time time NN cord-275664-qbafkxtr 70 18 points point NNS cord-275664-qbafkxtr 70 19 after after IN cord-275664-qbafkxtr 70 20 infection infection NN cord-275664-qbafkxtr 70 21 when when WRB cord-275664-qbafkxtr 70 22 compared compare VBN cord-275664-qbafkxtr 70 23 to to IN cord-275664-qbafkxtr 70 24 that that DT cord-275664-qbafkxtr 70 25 in in IN cord-275664-qbafkxtr 70 26 the the DT cord-275664-qbafkxtr 70 27 mock mock NN cord-275664-qbafkxtr 70 28 - - HYPH cord-275664-qbafkxtr 70 29 infected infect VBN cord-275664-qbafkxtr 70 30 cells cell NNS cord-275664-qbafkxtr 70 31 . . . cord-275664-qbafkxtr 71 1 Thus thus RB cord-275664-qbafkxtr 71 2 , , , cord-275664-qbafkxtr 71 3 these these DT cord-275664-qbafkxtr 71 4 data datum NNS cord-275664-qbafkxtr 71 5 indicate indicate VBP cord-275664-qbafkxtr 71 6 that that IN cord-275664-qbafkxtr 71 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 71 8 infection infection NN cord-275664-qbafkxtr 71 9 induces induce VBZ cord-275664-qbafkxtr 71 10 the the DT cord-275664-qbafkxtr 71 11 activation activation NN cord-275664-qbafkxtr 71 12 of of IN cord-275664-qbafkxtr 71 13 the the DT cord-275664-qbafkxtr 71 14 cellular cellular JJ cord-275664-qbafkxtr 71 15 signaling signal VBG cord-275664-qbafkxtr 71 16 pathway pathway NN cord-275664-qbafkxtr 71 17 mediated mediate VBN cord-275664-qbafkxtr 71 18 by by IN cord-275664-qbafkxtr 71 19 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 71 20 . . . cord-275664-qbafkxtr 72 1 To to TO cord-275664-qbafkxtr 72 2 determine determine VB cord-275664-qbafkxtr 72 3 whether whether IN cord-275664-qbafkxtr 72 4 PCV2 pcv2 JJ cord-275664-qbafkxtr 72 5 replication replication NN cord-275664-qbafkxtr 72 6 was be VBD cord-275664-qbafkxtr 72 7 required require VBN cord-275664-qbafkxtr 72 8 for for IN cord-275664-qbafkxtr 72 9 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 72 10 phosphorylation phosphorylation NN cord-275664-qbafkxtr 72 11 , , , cord-275664-qbafkxtr 72 12 we -PRON- PRP cord-275664-qbafkxtr 72 13 used use VBD cord-275664-qbafkxtr 72 14 the the DT cord-275664-qbafkxtr 72 15 FACE face NN cord-275664-qbafkxtr 72 16 assay assay NN cord-275664-qbafkxtr 72 17 to to TO cord-275664-qbafkxtr 72 18 examine examine VB cord-275664-qbafkxtr 72 19 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 73 1 phosphorylation phosphorylation NN cord-275664-qbafkxtr 73 2 in in IN cord-275664-qbafkxtr 73 3 the the DT cord-275664-qbafkxtr 73 4 cultured cultured JJ cord-275664-qbafkxtr 73 5 cells cell NNS cord-275664-qbafkxtr 73 6 when when WRB cord-275664-qbafkxtr 73 7 infected infect VBN cord-275664-qbafkxtr 73 8 with with IN cord-275664-qbafkxtr 73 9 a a DT cord-275664-qbafkxtr 73 10 UV UV NNP cord-275664-qbafkxtr 73 11 lightirradiated lightirradiated JJ cord-275664-qbafkxtr 73 12 virus virus NN cord-275664-qbafkxtr 73 13 sample sample NN cord-275664-qbafkxtr 73 14 . . . cord-275664-qbafkxtr 74 1 PK15 PK15 NNP cord-275664-qbafkxtr 74 2 cells cell NNS cord-275664-qbafkxtr 74 3 were be VBD cord-275664-qbafkxtr 74 4 infected infect VBN cord-275664-qbafkxtr 74 5 an an DT cord-275664-qbafkxtr 74 6 unirradiated unirradiated JJ cord-275664-qbafkxtr 74 7 or or CC cord-275664-qbafkxtr 74 8 irradiated irradiate VBN cord-275664-qbafkxtr 74 9 virus virus NN cord-275664-qbafkxtr 74 10 samples sample NNS cord-275664-qbafkxtr 74 11 for for IN cord-275664-qbafkxtr 74 12 72 72 CD cord-275664-qbafkxtr 74 13 h h NN cord-275664-qbafkxtr 74 14 postinfection postinfection NN cord-275664-qbafkxtr 74 15 . . . cord-275664-qbafkxtr 75 1 As as IN cord-275664-qbafkxtr 75 2 shown show VBN cord-275664-qbafkxtr 75 3 in in IN cord-275664-qbafkxtr 75 4 Fig Fig NNP cord-275664-qbafkxtr 75 5 . . . cord-275664-qbafkxtr 76 1 2 2 LS cord-275664-qbafkxtr 76 2 , , , cord-275664-qbafkxtr 76 3 no no DT cord-275664-qbafkxtr 76 4 significant significant JJ cord-275664-qbafkxtr 76 5 increase increase NN cord-275664-qbafkxtr 76 6 in in IN cord-275664-qbafkxtr 76 7 activation activation NN cord-275664-qbafkxtr 76 8 of of IN cord-275664-qbafkxtr 76 9 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 76 10 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 76 11 was be VBD cord-275664-qbafkxtr 76 12 observed observe VBN cord-275664-qbafkxtr 76 13 in in IN cord-275664-qbafkxtr 76 14 the the DT cord-275664-qbafkxtr 76 15 UV uv NN cord-275664-qbafkxtr 76 16 - - HYPH cord-275664-qbafkxtr 76 17 irradiated irradiate VBN cord-275664-qbafkxtr 76 18 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 76 19 cells cell NNS cord-275664-qbafkxtr 76 20 as as IN cord-275664-qbafkxtr 76 21 compared compare VBN cord-275664-qbafkxtr 76 22 to to IN cord-275664-qbafkxtr 76 23 that that DT cord-275664-qbafkxtr 76 24 in in IN cord-275664-qbafkxtr 76 25 the the DT cord-275664-qbafkxtr 76 26 mock mock NN cord-275664-qbafkxtr 76 27 - - HYPH cord-275664-qbafkxtr 76 28 infected infect VBN cord-275664-qbafkxtr 76 29 cells cell NNS cord-275664-qbafkxtr 76 30 . . . cord-275664-qbafkxtr 77 1 In in IN cord-275664-qbafkxtr 77 2 contrast contrast NN cord-275664-qbafkxtr 77 3 , , , cord-275664-qbafkxtr 77 4 the the DT cord-275664-qbafkxtr 77 5 levels level NNS cord-275664-qbafkxtr 77 6 of of IN cord-275664-qbafkxtr 77 7 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 77 8 phosphorylation phosphorylation NN cord-275664-qbafkxtr 77 9 increased increase VBN cord-275664-qbafkxtr 77 10 by by IN cord-275664-qbafkxtr 77 11 3.5-fold 3.5-fold CD cord-275664-qbafkxtr 77 12 when when WRB cord-275664-qbafkxtr 77 13 infected infect VBN cord-275664-qbafkxtr 77 14 with with IN cord-275664-qbafkxtr 77 15 the the DT cord-275664-qbafkxtr 77 16 unirradiated unirradiated JJ cord-275664-qbafkxtr 77 17 PCV2 PCV2 NNP cord-275664-qbafkxtr 77 18 at at IN cord-275664-qbafkxtr 77 19 72 72 CD cord-275664-qbafkxtr 77 20 h h NN cord-275664-qbafkxtr 77 21 postinfection postinfection NN cord-275664-qbafkxtr 77 22 . . . cord-275664-qbafkxtr 78 1 The the DT cord-275664-qbafkxtr 78 2 levels level NNS cord-275664-qbafkxtr 78 3 of of IN cord-275664-qbafkxtr 78 4 total total JJ cord-275664-qbafkxtr 78 5 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 78 6 remain remain VBP cord-275664-qbafkxtr 78 7 unchanged unchanged JJ cord-275664-qbafkxtr 78 8 in in IN cord-275664-qbafkxtr 78 9 the the DT cord-275664-qbafkxtr 78 10 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 78 11 cells cell NNS cord-275664-qbafkxtr 78 12 when when WRB cord-275664-qbafkxtr 78 13 compared compare VBN cord-275664-qbafkxtr 78 14 to to IN cord-275664-qbafkxtr 78 15 that that DT cord-275664-qbafkxtr 78 16 PCV2-infected PCV2-infected NNP cord-275664-qbafkxtr 78 17 cells cell NNS cord-275664-qbafkxtr 78 18 24 24 CD cord-275664-qbafkxtr 78 19 , , , cord-275664-qbafkxtr 78 20 48 48 CD cord-275664-qbafkxtr 78 21 , , , cord-275664-qbafkxtr 78 22 72 72 CD cord-275664-qbafkxtr 78 23 , , , cord-275664-qbafkxtr 78 24 and and CC cord-275664-qbafkxtr 78 25 96 96 CD cord-275664-qbafkxtr 78 26 h h NN cord-275664-qbafkxtr 78 27 were be VBD cord-275664-qbafkxtr 78 28 harvested harvest VBN cord-275664-qbafkxtr 78 29 , , , cord-275664-qbafkxtr 78 30 whole whole JJ cord-275664-qbafkxtr 78 31 cell cell NN cord-275664-qbafkxtr 78 32 lysates lysate NNS cord-275664-qbafkxtr 78 33 were be VBD cord-275664-qbafkxtr 78 34 prepared prepare VBN cord-275664-qbafkxtr 78 35 and and CC cord-275664-qbafkxtr 78 36 resolved resolve VBN cord-275664-qbafkxtr 78 37 by by IN cord-275664-qbafkxtr 78 38 SDS SDS NNP cord-275664-qbafkxtr 78 39 - - HYPH cord-275664-qbafkxtr 78 40 PAGE PAGE NNP cord-275664-qbafkxtr 78 41 , , , cord-275664-qbafkxtr 78 42 transferred transfer VBN cord-275664-qbafkxtr 78 43 to to IN cord-275664-qbafkxtr 78 44 nitrocellulose nitrocellulose JJ cord-275664-qbafkxtr 78 45 membranes membrane NNS cord-275664-qbafkxtr 78 46 , , , cord-275664-qbafkxtr 78 47 and and CC cord-275664-qbafkxtr 78 48 immunoblotted immunoblotte VBN cord-275664-qbafkxtr 78 49 by by IN cord-275664-qbafkxtr 78 50 using use VBG cord-275664-qbafkxtr 78 51 antibody antibody NN cord-275664-qbafkxtr 78 52 specific specific JJ cord-275664-qbafkxtr 78 53 for for IN cord-275664-qbafkxtr 78 54 ERK2 ERK2 NNP cord-275664-qbafkxtr 78 55 , , , cord-275664-qbafkxtr 78 56 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 78 57 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 78 58 , , , cord-275664-qbafkxtr 78 59 as as RB cord-275664-qbafkxtr 78 60 well well RB cord-275664-qbafkxtr 78 61 as as IN cord-275664-qbafkxtr 78 62 PCV2 pcv2 JJ cord-275664-qbafkxtr 78 63 viral viral JJ cord-275664-qbafkxtr 78 64 capsid capsid NN cord-275664-qbafkxtr 78 65 protein protein NN cord-275664-qbafkxtr 78 66 ORF2 ORF2 NNP cord-275664-qbafkxtr 78 67 . . . cord-275664-qbafkxtr 79 1 The the DT cord-275664-qbafkxtr 79 2 amounts amount NNS cord-275664-qbafkxtr 79 3 of of IN cord-275664-qbafkxtr 79 4 β β NN cord-275664-qbafkxtr 79 5 - - HYPH cord-275664-qbafkxtr 79 6 actin actin NN cord-275664-qbafkxtr 79 7 were be VBD cord-275664-qbafkxtr 79 8 also also RB cord-275664-qbafkxtr 79 9 assessed assess VBN cord-275664-qbafkxtr 79 10 to to TO cord-275664-qbafkxtr 79 11 monitor monitor VB cord-275664-qbafkxtr 79 12 the the DT cord-275664-qbafkxtr 79 13 equal equal JJ cord-275664-qbafkxtr 79 14 loadings loading NNS cord-275664-qbafkxtr 79 15 of of IN cord-275664-qbafkxtr 79 16 protein protein NN cord-275664-qbafkxtr 79 17 extracts extract NNS cord-275664-qbafkxtr 79 18 . . . cord-275664-qbafkxtr 80 1 ( ( -LRB- cord-275664-qbafkxtr 80 2 B b NN cord-275664-qbafkxtr 80 3 ) ) -RRB- cord-275664-qbafkxtr 80 4 ERK1/2 erk1/2 CD cord-275664-qbafkxtr 80 5 activation activation NN cord-275664-qbafkxtr 80 6 induced induce VBN cord-275664-qbafkxtr 80 7 by by IN cord-275664-qbafkxtr 80 8 PCV2 pcv2 JJ cord-275664-qbafkxtr 80 9 infection infection NN cord-275664-qbafkxtr 80 10 was be VBD cord-275664-qbafkxtr 80 11 quantitatively quantitatively RB cord-275664-qbafkxtr 80 12 determined determine VBN cord-275664-qbafkxtr 80 13 using use VBG cord-275664-qbafkxtr 80 14 FACS FACS NNP cord-275664-qbafkxtr 80 15 assay assay NN cord-275664-qbafkxtr 80 16 . . . cord-275664-qbafkxtr 81 1 PK15 PK15 NNP cord-275664-qbafkxtr 81 2 cells cell NNS cord-275664-qbafkxtr 81 3 were be VBD cord-275664-qbafkxtr 81 4 fixed fix VBN cord-275664-qbafkxtr 81 5 at at IN cord-275664-qbafkxtr 81 6 the the DT cord-275664-qbafkxtr 81 7 indicated indicate VBN cord-275664-qbafkxtr 81 8 time time NN cord-275664-qbafkxtr 81 9 points point NNS cord-275664-qbafkxtr 81 10 with with IN cord-275664-qbafkxtr 81 11 4 4 CD cord-275664-qbafkxtr 81 12 % % NN cord-275664-qbafkxtr 81 13 formaldehyde formaldehyde NN cord-275664-qbafkxtr 81 14 and and CC cord-275664-qbafkxtr 81 15 incubated incubate VBN cord-275664-qbafkxtr 81 16 with with IN cord-275664-qbafkxtr 81 17 normal normal JJ cord-275664-qbafkxtr 81 18 or or CC cord-275664-qbafkxtr 81 19 anti anti JJ cord-275664-qbafkxtr 81 20 - - JJ cord-275664-qbafkxtr 81 21 phospho phospho JJ cord-275664-qbafkxtr 81 22 - - HYPH cord-275664-qbafkxtr 81 23 specific specific JJ cord-275664-qbafkxtr 81 24 antibodies antibody NNS cord-275664-qbafkxtr 81 25 directed direct VBN cord-275664-qbafkxtr 81 26 against against IN cord-275664-qbafkxtr 81 27 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 81 28 followed follow VBN cord-275664-qbafkxtr 81 29 by by IN cord-275664-qbafkxtr 81 30 HRP HRP NNP cord-275664-qbafkxtr 81 31 - - HYPH cord-275664-qbafkxtr 81 32 conjugated conjugate VBN cord-275664-qbafkxtr 81 33 IgG IgG NNP cord-275664-qbafkxtr 81 34 antibodies antibody NNS cord-275664-qbafkxtr 81 35 . . . cord-275664-qbafkxtr 82 1 Total total JJ cord-275664-qbafkxtr 82 2 and and CC cord-275664-qbafkxtr 82 3 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 82 4 ERK ERK NNP cord-275664-qbafkxtr 82 5 were be VBD cord-275664-qbafkxtr 82 6 each each DT cord-275664-qbafkxtr 82 7 assayed assay VBN cord-275664-qbafkxtr 82 8 in in IN cord-275664-qbafkxtr 82 9 triplicate triplicate NN cord-275664-qbafkxtr 82 10 . . . cord-275664-qbafkxtr 83 1 Cell cell NN cord-275664-qbafkxtr 83 2 numbers number NNS cord-275664-qbafkxtr 83 3 were be VBD cord-275664-qbafkxtr 83 4 normalized normalize VBN cord-275664-qbafkxtr 83 5 using use VBG cord-275664-qbafkxtr 83 6 crystal crystal NN cord-275664-qbafkxtr 83 7 violet violet NN cord-275664-qbafkxtr 83 8 . . . cord-275664-qbafkxtr 84 1 These these DT cord-275664-qbafkxtr 84 2 results result NNS cord-275664-qbafkxtr 84 3 are be VBP cord-275664-qbafkxtr 84 4 representative representative JJ cord-275664-qbafkxtr 84 5 of of IN cord-275664-qbafkxtr 84 6 three three CD cord-275664-qbafkxtr 84 7 independent independent JJ cord-275664-qbafkxtr 84 8 experiments experiment NNS cord-275664-qbafkxtr 84 9 . . . cord-275664-qbafkxtr 85 1 Values value NNS cord-275664-qbafkxtr 85 2 are be VBP cord-275664-qbafkxtr 85 3 shown show VBN cord-275664-qbafkxtr 85 4 as as IN cord-275664-qbafkxtr 85 5 the the DT cord-275664-qbafkxtr 85 6 mean mean JJ cord-275664-qbafkxtr 85 7 ± ± NNP cord-275664-qbafkxtr 85 8 SD SD NNP cord-275664-qbafkxtr 85 9 from from IN cord-275664-qbafkxtr 85 10 triplicate triplicate JJ cord-275664-qbafkxtr 85 11 wells well NNS cord-275664-qbafkxtr 85 12 . . . cord-275664-qbafkxtr 86 1 p- p- UH cord-275664-qbafkxtr 86 2 , , , cord-275664-qbafkxtr 87 1 phospho- phospho- NNP cord-275664-qbafkxtr 87 2 . . . cord-275664-qbafkxtr 88 1 in in IN cord-275664-qbafkxtr 88 2 the the DT cord-275664-qbafkxtr 88 3 mock mock NN cord-275664-qbafkxtr 88 4 - - HYPH cord-275664-qbafkxtr 88 5 infected infect VBN cord-275664-qbafkxtr 88 6 cells cell NNS cord-275664-qbafkxtr 88 7 . . . cord-275664-qbafkxtr 89 1 The the DT cord-275664-qbafkxtr 89 2 result result NN cord-275664-qbafkxtr 89 3 demonstrated demonstrate VBD cord-275664-qbafkxtr 89 4 that that IN cord-275664-qbafkxtr 89 5 PCV2 pcv2 JJ cord-275664-qbafkxtr 89 6 replication replication NN cord-275664-qbafkxtr 89 7 was be VBD cord-275664-qbafkxtr 89 8 required require VBN cord-275664-qbafkxtr 89 9 for for IN cord-275664-qbafkxtr 89 10 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 89 11 phosphorylation phosphorylation NN cord-275664-qbafkxtr 89 12 . . . cord-275664-qbafkxtr 90 1 To to TO cord-275664-qbafkxtr 90 2 confirm confirm VB cord-275664-qbafkxtr 90 3 that that IN cord-275664-qbafkxtr 90 4 phosphorylation phosphorylation NN cord-275664-qbafkxtr 90 5 of of IN cord-275664-qbafkxtr 90 6 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 91 1 following follow VBG cord-275664-qbafkxtr 91 2 PCV2 pcv2 JJ cord-275664-qbafkxtr 91 3 infection infection NN cord-275664-qbafkxtr 91 4 truly truly RB cord-275664-qbafkxtr 91 5 activated activate VBD cord-275664-qbafkxtr 91 6 its -PRON- PRP$ cord-275664-qbafkxtr 91 7 downstream downstream NN cord-275664-qbafkxtr 91 8 substrate substrate NN cord-275664-qbafkxtr 91 9 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 91 10 , , , cord-275664-qbafkxtr 91 11 the the DT cord-275664-qbafkxtr 91 12 phosphorylation phosphorylation NN cord-275664-qbafkxtr 91 13 of of IN cord-275664-qbafkxtr 91 14 the the DT cord-275664-qbafkxtr 91 15 transcription transcription NN cord-275664-qbafkxtr 91 16 factor factor NN cord-275664-qbafkxtr 91 17 was be VBD cord-275664-qbafkxtr 91 18 measured measure VBN cord-275664-qbafkxtr 91 19 in in IN cord-275664-qbafkxtr 91 20 the the DT cord-275664-qbafkxtr 91 21 infected infected JJ cord-275664-qbafkxtr 91 22 cells cell NNS cord-275664-qbafkxtr 91 23 by by IN cord-275664-qbafkxtr 91 24 Western western JJ cord-275664-qbafkxtr 91 25 blotting blotting NN cord-275664-qbafkxtr 91 26 . . . cord-275664-qbafkxtr 92 1 No no DT cord-275664-qbafkxtr 92 2 activation activation NN cord-275664-qbafkxtr 92 3 of of IN cord-275664-qbafkxtr 92 4 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 92 5 was be VBD cord-275664-qbafkxtr 92 6 detected detect VBN cord-275664-qbafkxtr 92 7 in in IN cord-275664-qbafkxtr 92 8 the the DT cord-275664-qbafkxtr 92 9 mock mock NN cord-275664-qbafkxtr 92 10 - - HYPH cord-275664-qbafkxtr 92 11 infected infected JJ cord-275664-qbafkxtr 92 12 PK15 PK15 NNP cord-275664-qbafkxtr 92 13 cells cell NNS cord-275664-qbafkxtr 92 14 . . . cord-275664-qbafkxtr 93 1 Sustained sustained JJ cord-275664-qbafkxtr 93 2 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 93 3 activation activation NN cord-275664-qbafkxtr 93 4 after after IN cord-275664-qbafkxtr 93 5 PCV2 pcv2 JJ cord-275664-qbafkxtr 93 6 infection infection NN cord-275664-qbafkxtr 93 7 leads lead VBZ cord-275664-qbafkxtr 93 8 to to IN cord-275664-qbafkxtr 93 9 prolonged prolonged JJ cord-275664-qbafkxtr 93 10 phosphorylation phosphorylation NN cord-275664-qbafkxtr 93 11 of of IN cord-275664-qbafkxtr 94 1 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 94 2 ( ( -LRB- cord-275664-qbafkxtr 94 3 Fig Fig NNP cord-275664-qbafkxtr 94 4 . . . cord-275664-qbafkxtr 94 5 3A 3A NNP cord-275664-qbafkxtr 94 6 ) ) -RRB- cord-275664-qbafkxtr 94 7 , , , cord-275664-qbafkxtr 94 8 with with IN cord-275664-qbafkxtr 94 9 kinetics kinetic NNS cord-275664-qbafkxtr 94 10 that that WDT cord-275664-qbafkxtr 94 11 paralleled parallel VBD cord-275664-qbafkxtr 94 12 those those DT cord-275664-qbafkxtr 94 13 observed observe VBN cord-275664-qbafkxtr 94 14 for for IN cord-275664-qbafkxtr 94 15 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 94 16 . . . cord-275664-qbafkxtr 95 1 Cells cell NNS cord-275664-qbafkxtr 95 2 exposed expose VBN cord-275664-qbafkxtr 95 3 to to IN cord-275664-qbafkxtr 95 4 the the DT cord-275664-qbafkxtr 95 5 UV uv NN cord-275664-qbafkxtr 95 6 - - HYPH cord-275664-qbafkxtr 95 7 irradiated irradiate VBN cord-275664-qbafkxtr 95 8 virus virus NN cord-275664-qbafkxtr 95 9 sample sample NN cord-275664-qbafkxtr 95 10 failed fail VBD cord-275664-qbafkxtr 95 11 to to TO cord-275664-qbafkxtr 95 12 phosphorylate phosphorylate VB cord-275664-qbafkxtr 95 13 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 95 14 ( ( -LRB- cord-275664-qbafkxtr 95 15 Fig Fig NNP cord-275664-qbafkxtr 95 16 . . . cord-275664-qbafkxtr 95 17 3B 3B NNP cord-275664-qbafkxtr 95 18 ) ) -RRB- cord-275664-qbafkxtr 95 19 . . . cord-275664-qbafkxtr 96 1 In in IN cord-275664-qbafkxtr 96 2 addition addition NN cord-275664-qbafkxtr 96 3 , , , cord-275664-qbafkxtr 96 4 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 96 5 phosphorylation phosphorylation NN cord-275664-qbafkxtr 96 6 was be VBD cord-275664-qbafkxtr 96 7 specifically specifically RB cord-275664-qbafkxtr 96 8 impaired impair VBN cord-275664-qbafkxtr 96 9 by by IN cord-275664-qbafkxtr 96 10 preincubation preincubation NN cord-275664-qbafkxtr 96 11 with with IN cord-275664-qbafkxtr 96 12 MEK1/2 MEK1/2 NNP cord-275664-qbafkxtr 97 1 inhibitor inhibitor NN cord-275664-qbafkxtr 97 2 U0126 U0126 NNP cord-275664-qbafkxtr 97 3 at at IN cord-275664-qbafkxtr 97 4 20 20 CD cord-275664-qbafkxtr 97 5 μM μM NNP cord-275664-qbafkxtr 97 6 ( ( -LRB- cord-275664-qbafkxtr 97 7 Fig fig NN cord-275664-qbafkxtr 97 8 . . . cord-275664-qbafkxtr 97 9 3B 3B NNP cord-275664-qbafkxtr 97 10 ) ) -RRB- cord-275664-qbafkxtr 97 11 . . . cord-275664-qbafkxtr 98 1 The the DT cord-275664-qbafkxtr 98 2 result result NN cord-275664-qbafkxtr 98 3 demonstrated demonstrate VBD cord-275664-qbafkxtr 98 4 that that IN cord-275664-qbafkxtr 98 5 activation activation NN cord-275664-qbafkxtr 98 6 of of IN cord-275664-qbafkxtr 98 7 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 98 8 induced induce VBN cord-275664-qbafkxtr 98 9 by by IN cord-275664-qbafkxtr 98 10 PCV2 pcv2 JJ cord-275664-qbafkxtr 98 11 infection infection NN cord-275664-qbafkxtr 98 12 is be VBZ cord-275664-qbafkxtr 98 13 possibly possibly RB cord-275664-qbafkxtr 98 14 through through IN cord-275664-qbafkxtr 98 15 the the DT cord-275664-qbafkxtr 98 16 activation activation NN cord-275664-qbafkxtr 98 17 of of IN cord-275664-qbafkxtr 98 18 its -PRON- PRP$ cord-275664-qbafkxtr 98 19 downstream downstream JJ cord-275664-qbafkxtr 98 20 components component NNS cord-275664-qbafkxtr 98 21 such such JJ cord-275664-qbafkxtr 98 22 as as IN cord-275664-qbafkxtr 98 23 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 98 24 . . . cord-275664-qbafkxtr 99 1 To to TO cord-275664-qbafkxtr 99 2 determine determine VB cord-275664-qbafkxtr 99 3 whether whether IN cord-275664-qbafkxtr 99 4 activated activate VBN cord-275664-qbafkxtr 99 5 ERK ERK NNP cord-275664-qbafkxtr 99 6 plays play VBZ cord-275664-qbafkxtr 99 7 any any DT cord-275664-qbafkxtr 99 8 role role NN cord-275664-qbafkxtr 99 9 in in IN cord-275664-qbafkxtr 99 10 the the DT cord-275664-qbafkxtr 99 11 replication replication NN cord-275664-qbafkxtr 99 12 of of IN cord-275664-qbafkxtr 99 13 PCV2 PCV2 NNP cord-275664-qbafkxtr 99 14 , , , cord-275664-qbafkxtr 99 15 we -PRON- PRP cord-275664-qbafkxtr 99 16 examined examine VBD cord-275664-qbafkxtr 99 17 the the DT cord-275664-qbafkxtr 99 18 effect effect NN cord-275664-qbafkxtr 99 19 of of IN cord-275664-qbafkxtr 99 20 the the DT cord-275664-qbafkxtr 99 21 kinase kinase NN cord-275664-qbafkxtr 99 22 on on IN cord-275664-qbafkxtr 99 23 progeny progeny JJ cord-275664-qbafkxtr 99 24 virus virus NN cord-275664-qbafkxtr 99 25 production production NN cord-275664-qbafkxtr 99 26 in in IN cord-275664-qbafkxtr 99 27 the the DT cord-275664-qbafkxtr 99 28 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 99 29 PK15 PK15 NNP cord-275664-qbafkxtr 99 30 cells cell NNS cord-275664-qbafkxtr 99 31 by by IN cord-275664-qbafkxtr 99 32 blocking block VBG cord-275664-qbafkxtr 99 33 ERK ERK NNP cord-275664-qbafkxtr 99 34 activation activation NN cord-275664-qbafkxtr 99 35 . . . cord-275664-qbafkxtr 100 1 We -PRON- PRP cord-275664-qbafkxtr 100 2 infected infect VBD cord-275664-qbafkxtr 100 3 PK15 PK15 NNP cord-275664-qbafkxtr 100 4 cells cell NNS cord-275664-qbafkxtr 100 5 with with IN cord-275664-qbafkxtr 100 6 PCV2 PCV2 NNP cord-275664-qbafkxtr 100 7 in in IN cord-275664-qbafkxtr 100 8 the the DT cord-275664-qbafkxtr 100 9 presence presence NN cord-275664-qbafkxtr 100 10 of of IN cord-275664-qbafkxtr 100 11 the the DT cord-275664-qbafkxtr 100 12 inhibitor inhibitor NN cord-275664-qbafkxtr 100 13 U0126 U0126 NNP cord-275664-qbafkxtr 101 1 ( ( -LRB- cord-275664-qbafkxtr 101 2 5 5 CD cord-275664-qbafkxtr 101 3 - - SYM cord-275664-qbafkxtr 101 4 20 20 CD cord-275664-qbafkxtr 101 5 μM μM NNP cord-275664-qbafkxtr 101 6 ) ) -RRB- cord-275664-qbafkxtr 101 7 or or CC cord-275664-qbafkxtr 101 8 control control NN cord-275664-qbafkxtr 101 9 ( ( -LRB- cord-275664-qbafkxtr 101 10 DMSO DMSO NNP cord-275664-qbafkxtr 101 11 ) ) -RRB- cord-275664-qbafkxtr 101 12 , , , cord-275664-qbafkxtr 101 13 and and CC cord-275664-qbafkxtr 101 14 determined determine VBD cord-275664-qbafkxtr 101 15 the the DT cord-275664-qbafkxtr 101 16 viral viral JJ cord-275664-qbafkxtr 101 17 concentration concentration NN cord-275664-qbafkxtr 101 18 in in IN cord-275664-qbafkxtr 101 19 the the DT cord-275664-qbafkxtr 101 20 cell cell NN cord-275664-qbafkxtr 101 21 culture culture NN cord-275664-qbafkxtr 101 22 supernatant supernatant VBN cord-275664-qbafkxtr 101 23 72 72 CD cord-275664-qbafkxtr 101 24 h h NN cord-275664-qbafkxtr 101 25 after after IN cord-275664-qbafkxtr 101 26 infection infection NN cord-275664-qbafkxtr 101 27 . . . cord-275664-qbafkxtr 102 1 The the DT cord-275664-qbafkxtr 102 2 kinase kinase NN cord-275664-qbafkxtr 102 3 inhibitor inhibitor NN cord-275664-qbafkxtr 102 4 was be VBD cord-275664-qbafkxtr 102 5 also also RB cord-275664-qbafkxtr 102 6 present present JJ cord-275664-qbafkxtr 102 7 during during IN cord-275664-qbafkxtr 102 8 infection infection NN cord-275664-qbafkxtr 102 9 and and CC cord-275664-qbafkxtr 102 10 in in IN cord-275664-qbafkxtr 102 11 subsequent subsequent JJ cord-275664-qbafkxtr 102 12 incubation incubation NN cord-275664-qbafkxtr 102 13 periods period NNS cord-275664-qbafkxtr 102 14 . . . cord-275664-qbafkxtr 103 1 The the DT cord-275664-qbafkxtr 103 2 selected select VBN cord-275664-qbafkxtr 103 3 concentrations concentration NNS cord-275664-qbafkxtr 103 4 of of IN cord-275664-qbafkxtr 103 5 the the DT cord-275664-qbafkxtr 103 6 kinase kinase NN cord-275664-qbafkxtr 103 7 inhibitor inhibitor NN cord-275664-qbafkxtr 103 8 were be VBD cord-275664-qbafkxtr 103 9 tested test VBN cord-275664-qbafkxtr 103 10 to to TO cord-275664-qbafkxtr 103 11 show show VB cord-275664-qbafkxtr 103 12 their -PRON- PRP$ cord-275664-qbafkxtr 103 13 effectiveness effectiveness NN cord-275664-qbafkxtr 103 14 in in IN cord-275664-qbafkxtr 103 15 inhibiting inhibit VBG cord-275664-qbafkxtr 104 1 virus virus NN cord-275664-qbafkxtr 104 2 - - HYPH cord-275664-qbafkxtr 104 3 induced induce VBN cord-275664-qbafkxtr 104 4 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 104 5 activity activity NN cord-275664-qbafkxtr 104 6 by by IN cord-275664-qbafkxtr 104 7 the the DT cord-275664-qbafkxtr 104 8 FACS FACS NNP cord-275664-qbafkxtr 104 9 assay assay NN cord-275664-qbafkxtr 104 10 , , , cord-275664-qbafkxtr 104 11 which which WDT cord-275664-qbafkxtr 104 12 demonstrated demonstrate VBD cord-275664-qbafkxtr 104 13 that that IN cord-275664-qbafkxtr 104 14 activation activation NN cord-275664-qbafkxtr 104 15 of of IN cord-275664-qbafkxtr 104 16 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 104 17 reduced reduce VBN cord-275664-qbafkxtr 104 18 dose dose NN cord-275664-qbafkxtr 104 19 - - HYPH cord-275664-qbafkxtr 104 20 dependently dependently RB cord-275664-qbafkxtr 104 21 ( ( -LRB- cord-275664-qbafkxtr 104 22 data datum NNS cord-275664-qbafkxtr 104 23 not not RB cord-275664-qbafkxtr 104 24 shown show VBN cord-275664-qbafkxtr 104 25 ) ) -RRB- cord-275664-qbafkxtr 104 26 . . . cord-275664-qbafkxtr 105 1 Seventy seventy CD cord-275664-qbafkxtr 105 2 - - HYPH cord-275664-qbafkxtr 105 3 two two CD cord-275664-qbafkxtr 105 4 hours hour NNS cord-275664-qbafkxtr 105 5 postinfection postinfection NN cord-275664-qbafkxtr 105 6 supernatants supernatant NNS cord-275664-qbafkxtr 105 7 were be VBD cord-275664-qbafkxtr 105 8 collected collect VBN cord-275664-qbafkxtr 105 9 and and CC cord-275664-qbafkxtr 105 10 viral viral JJ cord-275664-qbafkxtr 105 11 production production NN cord-275664-qbafkxtr 105 12 was be VBD cord-275664-qbafkxtr 105 13 determined determine VBN cord-275664-qbafkxtr 105 14 by by IN cord-275664-qbafkxtr 105 15 the the DT cord-275664-qbafkxtr 105 16 IFA IFA NNP cord-275664-qbafkxtr 105 17 method method NN cord-275664-qbafkxtr 105 18 . . . cord-275664-qbafkxtr 106 1 The the DT cord-275664-qbafkxtr 106 2 inhibitor inhibitor NN cord-275664-qbafkxtr 106 3 U0126 U0126 NNP cord-275664-qbafkxtr 106 4 at at IN cord-275664-qbafkxtr 106 5 5 5 CD cord-275664-qbafkxtr 106 6 μM μm CD cord-275664-qbafkxtr 106 7 inhibited inhibit VBN cord-275664-qbafkxtr 106 8 PCV2 pcv2 JJ cord-275664-qbafkxtr 106 9 production production NN cord-275664-qbafkxtr 106 10 in in IN cord-275664-qbafkxtr 106 11 the the DT cord-275664-qbafkxtr 106 12 cultured cultured JJ cord-275664-qbafkxtr 106 13 cells cell NNS cord-275664-qbafkxtr 106 14 by by IN cord-275664-qbafkxtr 106 15 29 29 CD cord-275664-qbafkxtr 106 16 % % NN cord-275664-qbafkxtr 106 17 , , , cord-275664-qbafkxtr 106 18 compared compare VBN cord-275664-qbafkxtr 106 19 with with IN cord-275664-qbafkxtr 106 20 controls control NNS cord-275664-qbafkxtr 106 21 ( ( -LRB- cord-275664-qbafkxtr 106 22 Fig Fig NNP cord-275664-qbafkxtr 106 23 . . NNP cord-275664-qbafkxtr 106 24 4 4 CD cord-275664-qbafkxtr 106 25 ) ) -RRB- cord-275664-qbafkxtr 106 26 . . . cord-275664-qbafkxtr 107 1 Treatment treatment NN cord-275664-qbafkxtr 107 2 with with IN cord-275664-qbafkxtr 107 3 the the DT cord-275664-qbafkxtr 107 4 inhibitor inhibitor NN cord-275664-qbafkxtr 107 5 U0126 U0126 NNP cord-275664-qbafkxtr 108 1 at at IN cord-275664-qbafkxtr 108 2 10 10 CD cord-275664-qbafkxtr 108 3 μM μm CD cord-275664-qbafkxtr 108 4 and and CC cord-275664-qbafkxtr 108 5 20 20 CD cord-275664-qbafkxtr 108 6 μM μm CD cord-275664-qbafkxtr 109 1 reduced reduce VBN cord-275664-qbafkxtr 109 2 PCV2 pcv2 JJ cord-275664-qbafkxtr 109 3 growth growth NN cord-275664-qbafkxtr 109 4 by by IN cord-275664-qbafkxtr 109 5 73 73 CD cord-275664-qbafkxtr 109 6 % % NN cord-275664-qbafkxtr 109 7 and and CC cord-275664-qbafkxtr 109 8 92 92 CD cord-275664-qbafkxtr 109 9 % % NN cord-275664-qbafkxtr 109 10 , , , cord-275664-qbafkxtr 109 11 respectively respectively RB cord-275664-qbafkxtr 109 12 . . . cord-275664-qbafkxtr 110 1 As as IN cord-275664-qbafkxtr 110 2 expected expect VBN cord-275664-qbafkxtr 110 3 , , , cord-275664-qbafkxtr 110 4 the the DT cord-275664-qbafkxtr 110 5 UV uv NN cord-275664-qbafkxtr 110 6 - - HYPH cord-275664-qbafkxtr 110 7 irradiated irradiate VBN cord-275664-qbafkxtr 110 8 PCV2 PCV2 NNP cord-275664-qbafkxtr 110 9 failed fail VBD cord-275664-qbafkxtr 110 10 to to TO cord-275664-qbafkxtr 110 11 grow grow VB cord-275664-qbafkxtr 110 12 comparable comparable JJ cord-275664-qbafkxtr 110 13 to to IN cord-275664-qbafkxtr 110 14 those those DT cord-275664-qbafkxtr 110 15 seen see VBN cord-275664-qbafkxtr 110 16 in in IN cord-275664-qbafkxtr 110 17 the the DT cord-275664-qbafkxtr 110 18 mock mock NN cord-275664-qbafkxtr 110 19 - - HYPH cord-275664-qbafkxtr 110 20 infected infect VBN cord-275664-qbafkxtr 110 21 cells cell NNS cord-275664-qbafkxtr 110 22 ( ( -LRB- cord-275664-qbafkxtr 110 23 Fig Fig NNP cord-275664-qbafkxtr 110 24 . . NNP cord-275664-qbafkxtr 110 25 4 4 CD cord-275664-qbafkxtr 110 26 ) ) -RRB- cord-275664-qbafkxtr 110 27 . . . cord-275664-qbafkxtr 111 1 The the DT cord-275664-qbafkxtr 111 2 result result NN cord-275664-qbafkxtr 111 3 suggested suggest VBD cord-275664-qbafkxtr 111 4 that that IN cord-275664-qbafkxtr 111 5 ERK ERK NNP cord-275664-qbafkxtr 111 6 signal signal NN cord-275664-qbafkxtr 111 7 regulates regulate VBZ cord-275664-qbafkxtr 111 8 the the DT cord-275664-qbafkxtr 111 9 replication replication NN cord-275664-qbafkxtr 111 10 of of IN cord-275664-qbafkxtr 111 11 PCV2 PCV2 NNP cord-275664-qbafkxtr 111 12 in in IN cord-275664-qbafkxtr 111 13 PK15 PK15 NNP cord-275664-qbafkxtr 111 14 cells cell NNS cord-275664-qbafkxtr 111 15 . . . cord-275664-qbafkxtr 112 1 To to TO cord-275664-qbafkxtr 112 2 more more RBR cord-275664-qbafkxtr 112 3 specifically specifically RB cord-275664-qbafkxtr 112 4 identify identify VB cord-275664-qbafkxtr 112 5 the the DT cord-275664-qbafkxtr 112 6 stage stage NN cord-275664-qbafkxtr 112 7 of of IN cord-275664-qbafkxtr 112 8 PCV2 pcv2 JJ cord-275664-qbafkxtr 112 9 infection infection NN cord-275664-qbafkxtr 112 10 that that WDT cord-275664-qbafkxtr 112 11 was be VBD cord-275664-qbafkxtr 112 12 targeted target VBN cord-275664-qbafkxtr 112 13 by by IN cord-275664-qbafkxtr 112 14 the the DT cord-275664-qbafkxtr 112 15 inhibition inhibition NN cord-275664-qbafkxtr 112 16 of of IN cord-275664-qbafkxtr 112 17 ERK ERK NNP cord-275664-qbafkxtr 112 18 activation activation NN cord-275664-qbafkxtr 112 19 , , , cord-275664-qbafkxtr 112 20 we -PRON- PRP cord-275664-qbafkxtr 112 21 quantified quantify VBD cord-275664-qbafkxtr 112 22 PCV2 pcv2 JJ cord-275664-qbafkxtr 112 23 production production NN cord-275664-qbafkxtr 112 24 after after IN cord-275664-qbafkxtr 112 25 applying apply VBG cord-275664-qbafkxtr 112 26 the the DT cord-275664-qbafkxtr 112 27 inhibitor inhibitor NN cord-275664-qbafkxtr 112 28 U0126 U0126 NNP cord-275664-qbafkxtr 112 29 at at IN cord-275664-qbafkxtr 112 30 different different JJ cord-275664-qbafkxtr 112 31 time time NN cord-275664-qbafkxtr 112 32 points point NNS cord-275664-qbafkxtr 112 33 postinfection postinfection NN cord-275664-qbafkxtr 112 34 . . . cord-275664-qbafkxtr 113 1 The the DT cord-275664-qbafkxtr 113 2 cells cell NNS cord-275664-qbafkxtr 113 3 were be VBD cord-275664-qbafkxtr 113 4 incubated incubate VBN cord-275664-qbafkxtr 113 5 until until IN cord-275664-qbafkxtr 113 6 72 72 CD cord-275664-qbafkxtr 113 7 h h NN cord-275664-qbafkxtr 113 8 postinfection postinfection NN cord-275664-qbafkxtr 113 9 , , , cord-275664-qbafkxtr 113 10 and and CC cord-275664-qbafkxtr 113 11 the the DT cord-275664-qbafkxtr 113 12 supernatants supernatant NNS cord-275664-qbafkxtr 113 13 were be VBD cord-275664-qbafkxtr 113 14 collected collect VBN cord-275664-qbafkxtr 113 15 for for IN cord-275664-qbafkxtr 113 16 virus virus NN cord-275664-qbafkxtr 113 17 production production NN cord-275664-qbafkxtr 113 18 assay assay NN cord-275664-qbafkxtr 113 19 . . . cord-275664-qbafkxtr 114 1 As as IN cord-275664-qbafkxtr 114 2 shown show VBN cord-275664-qbafkxtr 114 3 in in IN cord-275664-qbafkxtr 114 4 Fig Fig NNP cord-275664-qbafkxtr 114 5 . . . cord-275664-qbafkxtr 115 1 5 5 LS cord-275664-qbafkxtr 115 2 , , , cord-275664-qbafkxtr 115 3 addition addition NN cord-275664-qbafkxtr 115 4 of of IN cord-275664-qbafkxtr 115 5 U0126 U0126 NNP cord-275664-qbafkxtr 115 6 at at IN cord-275664-qbafkxtr 115 7 the the DT cord-275664-qbafkxtr 115 8 time time NN cord-275664-qbafkxtr 115 9 of of IN cord-275664-qbafkxtr 115 10 infection infection NN cord-275664-qbafkxtr 115 11 led lead VBD cord-275664-qbafkxtr 115 12 to to IN cord-275664-qbafkxtr 115 13 a a DT cord-275664-qbafkxtr 115 14 91 91 CD cord-275664-qbafkxtr 115 15 % % NN cord-275664-qbafkxtr 115 16 inhibition inhibition NN cord-275664-qbafkxtr 115 17 in in IN cord-275664-qbafkxtr 115 18 virus virus NN cord-275664-qbafkxtr 115 19 production production NN cord-275664-qbafkxtr 115 20 as as IN cord-275664-qbafkxtr 115 21 compared compare VBN cord-275664-qbafkxtr 115 22 to to IN cord-275664-qbafkxtr 115 23 the the DT cord-275664-qbafkxtr 115 24 DMSO DMSO NNP cord-275664-qbafkxtr 115 25 - - HYPH cord-275664-qbafkxtr 115 26 treated treat VBN cord-275664-qbafkxtr 115 27 control control NN cord-275664-qbafkxtr 115 28 . . . cord-275664-qbafkxtr 116 1 Treatment treatment NN cord-275664-qbafkxtr 116 2 between between IN cord-275664-qbafkxtr 116 3 2 2 CD cord-275664-qbafkxtr 116 4 and and CC cord-275664-qbafkxtr 116 5 8 8 CD cord-275664-qbafkxtr 116 6 h h NN cord-275664-qbafkxtr 116 7 postinfection postinfection NN cord-275664-qbafkxtr 116 8 resulted result VBD cord-275664-qbafkxtr 116 9 in in IN cord-275664-qbafkxtr 116 10 significant significant JJ cord-275664-qbafkxtr 116 11 reduction reduction NN cord-275664-qbafkxtr 116 12 of of IN cord-275664-qbafkxtr 116 13 virus virus NN cord-275664-qbafkxtr 116 14 production production NN cord-275664-qbafkxtr 116 15 ( ( -LRB- cord-275664-qbafkxtr 116 16 73 73 CD cord-275664-qbafkxtr 116 17 % % NN cord-275664-qbafkxtr 116 18 to to IN cord-275664-qbafkxtr 116 19 28 28 CD cord-275664-qbafkxtr 116 20 % % NN cord-275664-qbafkxtr 116 21 reduction reduction NN cord-275664-qbafkxtr 116 22 compared compare VBN cord-275664-qbafkxtr 116 23 to to IN cord-275664-qbafkxtr 116 24 the the DT cord-275664-qbafkxtr 116 25 DMSO DMSO NNP cord-275664-qbafkxtr 116 26 - - HYPH cord-275664-qbafkxtr 116 27 treated treat VBN cord-275664-qbafkxtr 116 28 control control NN cord-275664-qbafkxtr 116 29 ) ) -RRB- cord-275664-qbafkxtr 116 30 . . . cord-275664-qbafkxtr 117 1 In in IN cord-275664-qbafkxtr 117 2 contrast contrast NN cord-275664-qbafkxtr 117 3 , , , cord-275664-qbafkxtr 117 4 no no DT cord-275664-qbafkxtr 117 5 significant significant JJ cord-275664-qbafkxtr 117 6 differences difference NNS cord-275664-qbafkxtr 117 7 in in IN cord-275664-qbafkxtr 117 8 viral viral JJ cord-275664-qbafkxtr 117 9 production production NN cord-275664-qbafkxtr 117 10 were be VBD cord-275664-qbafkxtr 117 11 observed observe VBN cord-275664-qbafkxtr 117 12 when when WRB cord-275664-qbafkxtr 117 13 the the DT cord-275664-qbafkxtr 117 14 inhibitor inhibitor NN cord-275664-qbafkxtr 117 15 was be VBD cord-275664-qbafkxtr 117 16 added add VBN cord-275664-qbafkxtr 117 17 at at IN cord-275664-qbafkxtr 117 18 12 12 CD cord-275664-qbafkxtr 117 19 or or CC cord-275664-qbafkxtr 117 20 24 24 CD cord-275664-qbafkxtr 117 21 h h NN cord-275664-qbafkxtr 117 22 postinfection postinfection NN cord-275664-qbafkxtr 117 23 . . . cord-275664-qbafkxtr 118 1 The the DT cord-275664-qbafkxtr 118 2 result result NN cord-275664-qbafkxtr 118 3 indicates indicate VBZ cord-275664-qbafkxtr 118 4 that that IN cord-275664-qbafkxtr 118 5 inhibition inhibition NN cord-275664-qbafkxtr 118 6 of of IN cord-275664-qbafkxtr 118 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 118 8 infectivity infectivity NN cord-275664-qbafkxtr 118 9 by by IN cord-275664-qbafkxtr 118 10 the the DT cord-275664-qbafkxtr 118 11 inhibitor inhibitor NN cord-275664-qbafkxtr 118 12 indeed indeed RB cord-275664-qbafkxtr 118 13 occurs occur VBZ cord-275664-qbafkxtr 118 14 at at IN cord-275664-qbafkxtr 118 15 the the DT cord-275664-qbafkxtr 118 16 early early JJ cord-275664-qbafkxtr 118 17 stage stage NN cord-275664-qbafkxtr 118 18 in in IN cord-275664-qbafkxtr 118 19 PCV2 pcv2 JJ cord-275664-qbafkxtr 118 20 infection infection NN cord-275664-qbafkxtr 118 21 . . . cord-275664-qbafkxtr 119 1 PCV2 pcv2 JJ cord-275664-qbafkxtr 119 2 replication replication NN cord-275664-qbafkxtr 119 3 is be VBZ cord-275664-qbafkxtr 119 4 required require VBN cord-275664-qbafkxtr 119 5 for for IN cord-275664-qbafkxtr 119 6 ERK ERK NNP cord-275664-qbafkxtr 119 7 phosphorylation phosphorylation NN cord-275664-qbafkxtr 119 8 . . . cord-275664-qbafkxtr 120 1 Monolayer Monolayer NNP cord-275664-qbafkxtr 120 2 PK15 PK15 NNP cord-275664-qbafkxtr 120 3 cells cell NNS cord-275664-qbafkxtr 120 4 were be VBD cord-275664-qbafkxtr 120 5 infected infect VBN cord-275664-qbafkxtr 120 6 with with IN cord-275664-qbafkxtr 120 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 120 8 strain strain NN cord-275664-qbafkxtr 120 9 BJW bjw NN cord-275664-qbafkxtr 120 10 at at IN cord-275664-qbafkxtr 120 11 a a DT cord-275664-qbafkxtr 120 12 MOI moi NN cord-275664-qbafkxtr 120 13 of of IN cord-275664-qbafkxtr 120 14 1 1 CD cord-275664-qbafkxtr 120 15 TCID TCID NNP cord-275664-qbafkxtr 120 16 50 50 CD cord-275664-qbafkxtr 120 17 . . . cord-275664-qbafkxtr 121 1 At at IN cord-275664-qbafkxtr 121 2 72 72 CD cord-275664-qbafkxtr 121 3 h h NN cord-275664-qbafkxtr 121 4 postinfection postinfection NN cord-275664-qbafkxtr 121 5 , , , cord-275664-qbafkxtr 121 6 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 121 7 cells cell NNS cord-275664-qbafkxtr 121 8 and and CC cord-275664-qbafkxtr 121 9 UV uv NN cord-275664-qbafkxtr 121 10 - - HYPH cord-275664-qbafkxtr 121 11 irradiated irradiate VBN cord-275664-qbafkxtr 121 12 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 121 13 cells cell NNS cord-275664-qbafkxtr 121 14 were be VBD cord-275664-qbafkxtr 121 15 fixed fix VBN cord-275664-qbafkxtr 121 16 with with IN cord-275664-qbafkxtr 121 17 4 4 CD cord-275664-qbafkxtr 121 18 % % NN cord-275664-qbafkxtr 121 19 formaldehyde formaldehyde NN cord-275664-qbafkxtr 121 20 . . . cord-275664-qbafkxtr 122 1 The the DT cord-275664-qbafkxtr 122 2 FACE face NN cord-275664-qbafkxtr 122 3 assay assay NN cord-275664-qbafkxtr 122 4 was be VBD cord-275664-qbafkxtr 122 5 performed perform VBN cord-275664-qbafkxtr 122 6 using use VBG cord-275664-qbafkxtr 122 7 normal normal JJ cord-275664-qbafkxtr 122 8 or or CC cord-275664-qbafkxtr 122 9 anti anti JJ cord-275664-qbafkxtr 122 10 - - JJ cord-275664-qbafkxtr 122 11 phospho phospho JJ cord-275664-qbafkxtr 122 12 - - HYPH cord-275664-qbafkxtr 122 13 specific specific JJ cord-275664-qbafkxtr 122 14 antibodies antibody NNS cord-275664-qbafkxtr 122 15 directed direct VBN cord-275664-qbafkxtr 122 16 against against IN cord-275664-qbafkxtr 122 17 ERK ERK NNP cord-275664-qbafkxtr 122 18 followed follow VBN cord-275664-qbafkxtr 122 19 by by IN cord-275664-qbafkxtr 122 20 HRP HRP NNP cord-275664-qbafkxtr 122 21 - - HYPH cord-275664-qbafkxtr 122 22 conjugated conjugate VBN cord-275664-qbafkxtr 122 23 IgG IgG NNP cord-275664-qbafkxtr 122 24 antibodies antibody NNS cord-275664-qbafkxtr 122 25 . . . cord-275664-qbafkxtr 123 1 Total total JJ cord-275664-qbafkxtr 123 2 and and CC cord-275664-qbafkxtr 123 3 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 123 4 ERK ERK NNP cord-275664-qbafkxtr 123 5 were be VBD cord-275664-qbafkxtr 123 6 each each DT cord-275664-qbafkxtr 123 7 assayed assay VBN cord-275664-qbafkxtr 123 8 in in IN cord-275664-qbafkxtr 123 9 triplicate triplicate NN cord-275664-qbafkxtr 123 10 . . . cord-275664-qbafkxtr 124 1 Cell cell NN cord-275664-qbafkxtr 124 2 numbers number NNS cord-275664-qbafkxtr 124 3 were be VBD cord-275664-qbafkxtr 124 4 normalized normalize VBN cord-275664-qbafkxtr 124 5 using use VBG cord-275664-qbafkxtr 124 6 crystal crystal NN cord-275664-qbafkxtr 124 7 violet violet NN cord-275664-qbafkxtr 124 8 . . . cord-275664-qbafkxtr 125 1 These these DT cord-275664-qbafkxtr 125 2 results result NNS cord-275664-qbafkxtr 125 3 are be VBP cord-275664-qbafkxtr 125 4 representative representative JJ cord-275664-qbafkxtr 125 5 of of IN cord-275664-qbafkxtr 125 6 three three CD cord-275664-qbafkxtr 125 7 independent independent JJ cord-275664-qbafkxtr 125 8 experiments experiment NNS cord-275664-qbafkxtr 125 9 . . . cord-275664-qbafkxtr 126 1 Values value NNS cord-275664-qbafkxtr 126 2 are be VBP cord-275664-qbafkxtr 126 3 shown show VBN cord-275664-qbafkxtr 126 4 as as IN cord-275664-qbafkxtr 126 5 the the DT cord-275664-qbafkxtr 126 6 mean mean JJ cord-275664-qbafkxtr 126 7 ± ± NNP cord-275664-qbafkxtr 126 8 SD SD NNP cord-275664-qbafkxtr 126 9 from from IN cord-275664-qbafkxtr 126 10 triplicate triplicate JJ cord-275664-qbafkxtr 126 11 wells well NNS cord-275664-qbafkxtr 126 12 . . . cord-275664-qbafkxtr 127 1 To to TO cord-275664-qbafkxtr 127 2 help help VB cord-275664-qbafkxtr 127 3 delineate delineate VB cord-275664-qbafkxtr 127 4 the the DT cord-275664-qbafkxtr 127 5 mechanism mechanism NN cord-275664-qbafkxtr 127 6 of of IN cord-275664-qbafkxtr 127 7 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 127 8 regulation regulation NN cord-275664-qbafkxtr 127 9 of of IN cord-275664-qbafkxtr 127 10 PCV2 pcv2 JJ cord-275664-qbafkxtr 127 11 infection infection NN cord-275664-qbafkxtr 127 12 , , , cord-275664-qbafkxtr 127 13 we -PRON- PRP cord-275664-qbafkxtr 127 14 examined examine VBD cord-275664-qbafkxtr 127 15 the the DT cord-275664-qbafkxtr 127 16 effect effect NN cord-275664-qbafkxtr 127 17 of of IN cord-275664-qbafkxtr 127 18 ERK ERK NNP cord-275664-qbafkxtr 127 19 inhibition inhibition NN cord-275664-qbafkxtr 127 20 on on IN cord-275664-qbafkxtr 127 21 viral viral JJ cord-275664-qbafkxtr 127 22 protein protein NN cord-275664-qbafkxtr 127 23 expression expression NN cord-275664-qbafkxtr 127 24 . . . cord-275664-qbafkxtr 128 1 PK15 PK15 NNP cord-275664-qbafkxtr 128 2 cells cell NNS cord-275664-qbafkxtr 128 3 were be VBD cord-275664-qbafkxtr 128 4 infected infect VBN cord-275664-qbafkxtr 128 5 with with IN cord-275664-qbafkxtr 128 6 PCV2 PCV2 NNP cord-275664-qbafkxtr 128 7 ( ( -LRB- cord-275664-qbafkxtr 128 8 MOI MOI NNP cord-275664-qbafkxtr 128 9 of of IN cord-275664-qbafkxtr 128 10 1 1 CD cord-275664-qbafkxtr 128 11 ) ) -RRB- cord-275664-qbafkxtr 128 12 in in IN cord-275664-qbafkxtr 128 13 the the DT cord-275664-qbafkxtr 128 14 presence presence NN cord-275664-qbafkxtr 128 15 or or CC cord-275664-qbafkxtr 128 16 absence absence NN cord-275664-qbafkxtr 128 17 of of IN cord-275664-qbafkxtr 128 18 U0126 U0126 NNP cord-275664-qbafkxtr 128 19 ( ( -LRB- cord-275664-qbafkxtr 128 20 20 20 CD cord-275664-qbafkxtr 128 21 μM μM NNP cord-275664-qbafkxtr 128 22 ) ) -RRB- cord-275664-qbafkxtr 128 23 , , , cord-275664-qbafkxtr 128 24 and and CC cord-275664-qbafkxtr 128 25 ORF2 ORF2 NNP cord-275664-qbafkxtr 128 26 protein protein NN cord-275664-qbafkxtr 128 27 expression expression NN cord-275664-qbafkxtr 128 28 was be VBD cord-275664-qbafkxtr 128 29 monitored monitor VBN cord-275664-qbafkxtr 128 30 by by IN cord-275664-qbafkxtr 128 31 a a DT cord-275664-qbafkxtr 128 32 fluorescence fluorescence NN cord-275664-qbafkxtr 128 33 microscopy microscopy NN cord-275664-qbafkxtr 128 34 . . . cord-275664-qbafkxtr 129 1 The the DT cord-275664-qbafkxtr 129 2 ORF2 ORF2 NNP cord-275664-qbafkxtr 129 3 protein protein NN cord-275664-qbafkxtr 129 4 expression expression NN cord-275664-qbafkxtr 129 5 was be VBD cord-275664-qbafkxtr 129 6 significantly significantly RB cord-275664-qbafkxtr 129 7 reduced reduce VBN cord-275664-qbafkxtr 129 8 when when WRB cord-275664-qbafkxtr 129 9 cells cell NNS cord-275664-qbafkxtr 129 10 were be VBD cord-275664-qbafkxtr 129 11 treated treat VBN cord-275664-qbafkxtr 129 12 with with IN cord-275664-qbafkxtr 129 13 U0126 U0126 NNP cord-275664-qbafkxtr 129 14 , , , cord-275664-qbafkxtr 129 15 as as IN cord-275664-qbafkxtr 129 16 demonstrated demonstrate VBN cord-275664-qbafkxtr 129 17 by by IN cord-275664-qbafkxtr 129 18 the the DT cord-275664-qbafkxtr 129 19 increased increase VBN cord-275664-qbafkxtr 129 20 number number NN cord-275664-qbafkxtr 129 21 of of IN cord-275664-qbafkxtr 129 22 PCV2-positive pcv2-positive JJ cord-275664-qbafkxtr 129 23 cells cell NNS cord-275664-qbafkxtr 129 24 observed observe VBN cord-275664-qbafkxtr 129 25 in in IN cord-275664-qbafkxtr 129 26 DMSO DMSO NNP cord-275664-qbafkxtr 129 27 - - HYPH cord-275664-qbafkxtr 129 28 treated treat VBN cord-275664-qbafkxtr 129 29 infected infected JJ cord-275664-qbafkxtr 129 30 cells cell NNS cord-275664-qbafkxtr 129 31 ( ( -LRB- cord-275664-qbafkxtr 129 32 Fig fig NN cord-275664-qbafkxtr 129 33 . . . cord-275664-qbafkxtr 129 34 6A 6a CD cord-275664-qbafkxtr 129 35 ) ) -RRB- cord-275664-qbafkxtr 129 36 . . . cord-275664-qbafkxtr 130 1 No no DT cord-275664-qbafkxtr 130 2 significant significant JJ cord-275664-qbafkxtr 130 3 differences difference NNS cord-275664-qbafkxtr 130 4 were be VBD cord-275664-qbafkxtr 130 5 seen see VBN cord-275664-qbafkxtr 130 6 in in IN cord-275664-qbafkxtr 130 7 the the DT cord-275664-qbafkxtr 130 8 ORF2 ORF2 NNP cord-275664-qbafkxtr 130 9 protein protein NN cord-275664-qbafkxtr 130 10 expression expression NN cord-275664-qbafkxtr 130 11 between between IN cord-275664-qbafkxtr 130 12 DMSOtreated dmsotreated JJ cord-275664-qbafkxtr 130 13 infected infected JJ cord-275664-qbafkxtr 130 14 cells cell NNS cord-275664-qbafkxtr 130 15 and and CC cord-275664-qbafkxtr 130 16 untreated untreated JJ cord-275664-qbafkxtr 130 17 infected infected JJ cord-275664-qbafkxtr 130 18 cells cell NNS cord-275664-qbafkxtr 130 19 ( ( -LRB- cord-275664-qbafkxtr 130 20 data datum NNS cord-275664-qbafkxtr 130 21 not not RB cord-275664-qbafkxtr 130 22 shown show VBN cord-275664-qbafkxtr 130 23 ) ) -RRB- cord-275664-qbafkxtr 130 24 . . . cord-275664-qbafkxtr 131 1 No no DT cord-275664-qbafkxtr 131 2 ORF2 ORF2 NNP cord-275664-qbafkxtr 131 3 protein protein NN cord-275664-qbafkxtr 131 4 expression expression NN cord-275664-qbafkxtr 131 5 was be VBD cord-275664-qbafkxtr 131 6 detected detect VBN cord-275664-qbafkxtr 131 7 in in IN cord-275664-qbafkxtr 131 8 the the DT cord-275664-qbafkxtr 131 9 UV uv NN cord-275664-qbafkxtr 131 10 - - HYPH cord-275664-qbafkxtr 131 11 irradiated irradiate VBN cord-275664-qbafkxtr 131 12 PCV2infected PCV2infected NNP cord-275664-qbafkxtr 131 13 as as RB cord-275664-qbafkxtr 131 14 well well RB cord-275664-qbafkxtr 131 15 as as IN cord-275664-qbafkxtr 131 16 mock mock JJ cord-275664-qbafkxtr 131 17 - - HYPH cord-275664-qbafkxtr 131 18 infected infect VBN cord-275664-qbafkxtr 131 19 cells cell NNS cord-275664-qbafkxtr 131 20 ( ( -LRB- cord-275664-qbafkxtr 131 21 Fig fig NN cord-275664-qbafkxtr 131 22 . . . cord-275664-qbafkxtr 131 23 6A 6a CD cord-275664-qbafkxtr 131 24 ) ) -RRB- cord-275664-qbafkxtr 131 25 . . . cord-275664-qbafkxtr 132 1 We -PRON- PRP cord-275664-qbafkxtr 132 2 then then RB cord-275664-qbafkxtr 132 3 examined examine VBD cord-275664-qbafkxtr 132 4 the the DT cord-275664-qbafkxtr 132 5 effect effect NN cord-275664-qbafkxtr 132 6 of of IN cord-275664-qbafkxtr 132 7 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 132 8 inhibition inhibition NN cord-275664-qbafkxtr 132 9 on on IN cord-275664-qbafkxtr 132 10 PCV2 pcv2 PRP$ cord-275664-qbafkxtr 132 11 viral viral JJ cord-275664-qbafkxtr 132 12 mRNA mRNA NNP cord-275664-qbafkxtr 132 13 synthesis synthesis NN cord-275664-qbafkxtr 132 14 , , , cord-275664-qbafkxtr 132 15 the the DT cord-275664-qbafkxtr 132 16 real real JJ cord-275664-qbafkxtr 132 17 - - HYPH cord-275664-qbafkxtr 132 18 time time NN cord-275664-qbafkxtr 132 19 RT RT NNP cord-275664-qbafkxtr 132 20 - - HYPH cord-275664-qbafkxtr 132 21 PCR PCR NNP cord-275664-qbafkxtr 132 22 analysis analysis NN cord-275664-qbafkxtr 132 23 was be VBD cord-275664-qbafkxtr 132 24 performed perform VBN cord-275664-qbafkxtr 132 25 with with IN cord-275664-qbafkxtr 132 26 RNA RNA NNP cord-275664-qbafkxtr 132 27 extracted extract VBN cord-275664-qbafkxtr 132 28 from from IN cord-275664-qbafkxtr 132 29 PK15 PK15 NNP cord-275664-qbafkxtr 132 30 cells cell NNS cord-275664-qbafkxtr 132 31 48 48 CD cord-275664-qbafkxtr 132 32 h h NN cord-275664-qbafkxtr 132 33 after after IN cord-275664-qbafkxtr 132 34 treatment treatment NN cord-275664-qbafkxtr 132 35 with with IN cord-275664-qbafkxtr 132 36 U0126 U0126 NNP cord-275664-qbafkxtr 132 37 at at IN cord-275664-qbafkxtr 132 38 20 20 CD cord-275664-qbafkxtr 133 1 μM. μM. NNP cord-275664-qbafkxtr 134 1 The the DT cord-275664-qbafkxtr 134 2 amount amount NN cord-275664-qbafkxtr 134 3 of of IN cord-275664-qbafkxtr 134 4 each each DT cord-275664-qbafkxtr 134 5 viral viral JJ cord-275664-qbafkxtr 134 6 mRNA mrna NN cord-275664-qbafkxtr 134 7 was be VBD cord-275664-qbafkxtr 134 8 normalized normalize VBN cord-275664-qbafkxtr 134 9 to to IN cord-275664-qbafkxtr 134 10 that that DT cord-275664-qbafkxtr 134 11 of of IN cord-275664-qbafkxtr 134 12 βactin βactin NNP cord-275664-qbafkxtr 134 13 mRNA mRNA NNP cord-275664-qbafkxtr 134 14 in in IN cord-275664-qbafkxtr 134 15 the the DT cord-275664-qbafkxtr 134 16 same same JJ cord-275664-qbafkxtr 134 17 sample sample NN cord-275664-qbafkxtr 134 18 . . . cord-275664-qbafkxtr 135 1 The the DT cord-275664-qbafkxtr 135 2 abundance abundance NN cord-275664-qbafkxtr 135 3 of of IN cord-275664-qbafkxtr 136 1 PCV2 PCV2 NNP cord-275664-qbafkxtr 136 2 mRNA mrna NN cord-275664-qbafkxtr 136 3 was be VBD cord-275664-qbafkxtr 136 4 significantly significantly RB cord-275664-qbafkxtr 136 5 decreased decrease VBN cord-275664-qbafkxtr 136 6 in in IN cord-275664-qbafkxtr 136 7 the the DT cord-275664-qbafkxtr 136 8 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 136 9 cells cell NNS cord-275664-qbafkxtr 136 10 with with IN cord-275664-qbafkxtr 136 11 U0126 U0126 NNP cord-275664-qbafkxtr 136 12 treatment treatment NN cord-275664-qbafkxtr 137 1 ( ( -LRB- cord-275664-qbafkxtr 137 2 Fig Fig NNP cord-275664-qbafkxtr 137 3 . . . cord-275664-qbafkxtr 137 4 6B 6b CD cord-275664-qbafkxtr 137 5 ) ) -RRB- cord-275664-qbafkxtr 137 6 . . . cord-275664-qbafkxtr 138 1 At at IN cord-275664-qbafkxtr 138 2 the the DT cord-275664-qbafkxtr 138 3 indicated indicate VBN cord-275664-qbafkxtr 138 4 time time NN cord-275664-qbafkxtr 138 5 point point NN cord-275664-qbafkxtr 138 6 , , , cord-275664-qbafkxtr 138 7 the the DT cord-275664-qbafkxtr 138 8 amount amount NN cord-275664-qbafkxtr 138 9 of of IN cord-275664-qbafkxtr 138 10 PCV2-specific PCV2-specific NNP cord-275664-qbafkxtr 138 11 mRNA mrna NN cord-275664-qbafkxtr 138 12 in in IN cord-275664-qbafkxtr 138 13 U0126-treated u0126-treated JJ cord-275664-qbafkxtr 138 14 cells cell NNS cord-275664-qbafkxtr 138 15 was be VBD cord-275664-qbafkxtr 138 16 approximately approximately RB cord-275664-qbafkxtr 138 17 2.6 2.6 CD cord-275664-qbafkxtr 138 18 % % NN cord-275664-qbafkxtr 138 19 that that DT cord-275664-qbafkxtr 138 20 of of IN cord-275664-qbafkxtr 138 21 untreated untreated JJ cord-275664-qbafkxtr 138 22 cells cell NNS cord-275664-qbafkxtr 138 23 . . . cord-275664-qbafkxtr 139 1 In in IN cord-275664-qbafkxtr 139 2 contrast contrast NN cord-275664-qbafkxtr 139 3 , , , cord-275664-qbafkxtr 139 4 the the DT cord-275664-qbafkxtr 139 5 mRNA mrna NN cord-275664-qbafkxtr 139 6 was be VBD cord-275664-qbafkxtr 139 7 detected detect VBN cord-275664-qbafkxtr 139 8 in in IN cord-275664-qbafkxtr 139 9 the the DT cord-275664-qbafkxtr 139 10 DMSO DMSO NNP cord-275664-qbafkxtr 139 11 - - HYPH cord-275664-qbafkxtr 139 12 treated treat VBN cord-275664-qbafkxtr 139 13 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 139 14 cells cell NNS cord-275664-qbafkxtr 139 15 as as IN cord-275664-qbafkxtr 139 16 seen see VBN cord-275664-qbafkxtr 139 17 in in IN cord-275664-qbafkxtr 139 18 that that DT cord-275664-qbafkxtr 139 19 in in IN cord-275664-qbafkxtr 139 20 the the DT cord-275664-qbafkxtr 139 21 PCV2-alonedinfected PCV2-alonedinfected NNP cord-275664-qbafkxtr 139 22 cells cell NNS cord-275664-qbafkxtr 139 23 ( ( -LRB- cord-275664-qbafkxtr 139 24 data datum NNS cord-275664-qbafkxtr 139 25 not not RB cord-275664-qbafkxtr 139 26 shown show VBN cord-275664-qbafkxtr 139 27 ) ) -RRB- cord-275664-qbafkxtr 139 28 . . . cord-275664-qbafkxtr 140 1 No no DT cord-275664-qbafkxtr 140 2 viral viral JJ cord-275664-qbafkxtr 140 3 mRNA mrna NN cord-275664-qbafkxtr 140 4 accumulation accumulation NN cord-275664-qbafkxtr 140 5 was be VBD cord-275664-qbafkxtr 140 6 detected detect VBN cord-275664-qbafkxtr 140 7 in in IN cord-275664-qbafkxtr 140 8 the the DT cord-275664-qbafkxtr 140 9 UV uv NN cord-275664-qbafkxtr 140 10 - - HYPH cord-275664-qbafkxtr 140 11 irradiated irradiate VBN cord-275664-qbafkxtr 140 12 PCV2-infected PCV2-infected NNP cord-275664-qbafkxtr 140 13 as as RB cord-275664-qbafkxtr 140 14 well well RB cord-275664-qbafkxtr 140 15 as as IN cord-275664-qbafkxtr 140 16 mock mock JJ cord-275664-qbafkxtr 140 17 - - HYPH cord-275664-qbafkxtr 140 18 infected infect VBN cord-275664-qbafkxtr 140 19 cells cell NNS cord-275664-qbafkxtr 141 1 ( ( -LRB- cord-275664-qbafkxtr 141 2 Fig Fig NNP cord-275664-qbafkxtr 141 3 . . . cord-275664-qbafkxtr 141 4 6B 6b CD cord-275664-qbafkxtr 141 5 ) ) -RRB- cord-275664-qbafkxtr 141 6 . . . cord-275664-qbafkxtr 142 1 The the DT cord-275664-qbafkxtr 142 2 results result NNS cord-275664-qbafkxtr 142 3 indicate indicate VBP cord-275664-qbafkxtr 142 4 that that IN cord-275664-qbafkxtr 142 5 inhibition inhibition NN cord-275664-qbafkxtr 142 6 of of IN cord-275664-qbafkxtr 142 7 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 142 8 activation activation NN cord-275664-qbafkxtr 142 9 did do VBD cord-275664-qbafkxtr 142 10 have have VB cord-275664-qbafkxtr 142 11 an an DT cord-275664-qbafkxtr 142 12 inhibitory inhibitory JJ cord-275664-qbafkxtr 142 13 effect effect NN cord-275664-qbafkxtr 142 14 on on IN cord-275664-qbafkxtr 142 15 viral viral JJ cord-275664-qbafkxtr 142 16 protein protein NN cord-275664-qbafkxtr 142 17 synthesis synthesis NN cord-275664-qbafkxtr 142 18 and and CC cord-275664-qbafkxtr 142 19 the the DT cord-275664-qbafkxtr 142 20 accumulation accumulation NN cord-275664-qbafkxtr 142 21 of of IN cord-275664-qbafkxtr 142 22 viral viral JJ cord-275664-qbafkxtr 142 23 - - HYPH cord-275664-qbafkxtr 142 24 specific specific JJ cord-275664-qbafkxtr 142 25 mRNA mrna NN cord-275664-qbafkxtr 142 26 in in IN cord-275664-qbafkxtr 142 27 the the DT cord-275664-qbafkxtr 142 28 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 142 29 cells cell NNS cord-275664-qbafkxtr 142 30 . . . cord-275664-qbafkxtr 143 1 Infection infection NN cord-275664-qbafkxtr 143 2 with with IN cord-275664-qbafkxtr 143 3 a a DT cord-275664-qbafkxtr 143 4 variety variety NN cord-275664-qbafkxtr 143 5 of of IN cord-275664-qbafkxtr 143 6 viruses virus NNS cord-275664-qbafkxtr 143 7 lead lead VBP cord-275664-qbafkxtr 143 8 to to IN cord-275664-qbafkxtr 143 9 the the DT cord-275664-qbafkxtr 143 10 perturbation perturbation NN cord-275664-qbafkxtr 143 11 of of IN cord-275664-qbafkxtr 143 12 host host NN cord-275664-qbafkxtr 143 13 cell cell NN cord-275664-qbafkxtr 143 14 signaling signal VBG cord-275664-qbafkxtr 143 15 pathways pathway NNS cord-275664-qbafkxtr 143 16 including include VBG cord-275664-qbafkxtr 143 17 ERK ERK NNP cord-275664-qbafkxtr 143 18 MAPK MAPK NNP cord-275664-qbafkxtr 143 19 cascade cascade NN cord-275664-qbafkxtr 143 20 , , , cord-275664-qbafkxtr 143 21 which which WDT cord-275664-qbafkxtr 143 22 can can MD cord-275664-qbafkxtr 143 23 affect affect VB cord-275664-qbafkxtr 143 24 cellular cellular JJ cord-275664-qbafkxtr 143 25 function function NN cord-275664-qbafkxtr 143 26 and and CC cord-275664-qbafkxtr 143 27 virus virus NN cord-275664-qbafkxtr 143 28 replication replication NN cord-275664-qbafkxtr 143 29 . . . cord-275664-qbafkxtr 144 1 In in IN cord-275664-qbafkxtr 144 2 the the DT cord-275664-qbafkxtr 144 3 present present JJ cord-275664-qbafkxtr 144 4 study study NN cord-275664-qbafkxtr 144 5 , , , cord-275664-qbafkxtr 144 6 we -PRON- PRP cord-275664-qbafkxtr 144 7 show show VBP cord-275664-qbafkxtr 144 8 that that IN cord-275664-qbafkxtr 144 9 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 144 10 MAPK MAPK NNP cord-275664-qbafkxtr 144 11 pathway pathway NN cord-275664-qbafkxtr 144 12 was be VBD cord-275664-qbafkxtr 144 13 activated activate VBN cord-275664-qbafkxtr 144 14 during during IN cord-275664-qbafkxtr 144 15 the the DT cord-275664-qbafkxtr 144 16 course course NN cord-275664-qbafkxtr 144 17 of of IN cord-275664-qbafkxtr 144 18 PCV2 pcv2 JJ cord-275664-qbafkxtr 144 19 infection infection NN cord-275664-qbafkxtr 144 20 in in IN cord-275664-qbafkxtr 144 21 PK15 PK15 NNP cord-275664-qbafkxtr 144 22 cells cell NNS cord-275664-qbafkxtr 144 23 ( ( -LRB- cord-275664-qbafkxtr 144 24 Figs fig NNS cord-275664-qbafkxtr 144 25 . . . cord-275664-qbafkxtr 145 1 1A 1A NNP cord-275664-qbafkxtr 145 2 and and CC cord-275664-qbafkxtr 145 3 B B NNP cord-275664-qbafkxtr 145 4 ) ) -RRB- cord-275664-qbafkxtr 145 5 , , , cord-275664-qbafkxtr 145 6 and and CC cord-275664-qbafkxtr 145 7 its -PRON- PRP$ cord-275664-qbafkxtr 145 8 activation activation NN cord-275664-qbafkxtr 145 9 was be VBD cord-275664-qbafkxtr 145 10 required require VBN cord-275664-qbafkxtr 145 11 for for IN cord-275664-qbafkxtr 145 12 viral viral JJ cord-275664-qbafkxtr 145 13 replication replication NN cord-275664-qbafkxtr 145 14 ( ( -LRB- cord-275664-qbafkxtr 145 15 Fig fig NN cord-275664-qbafkxtr 145 16 . . NNP cord-275664-qbafkxtr 145 17 2 2 CD cord-275664-qbafkxtr 145 18 ) ) -RRB- cord-275664-qbafkxtr 145 19 . . . cord-275664-qbafkxtr 146 1 Also also RB cord-275664-qbafkxtr 146 2 , , , cord-275664-qbafkxtr 146 3 we -PRON- PRP cord-275664-qbafkxtr 146 4 show show VBP cord-275664-qbafkxtr 146 5 that that IN cord-275664-qbafkxtr 146 6 PCV2 pcv2 CC cord-275664-qbafkxtr 146 7 infection infection NN cord-275664-qbafkxtr 146 8 induced induce VBD cord-275664-qbafkxtr 146 9 the the DT cord-275664-qbafkxtr 146 10 transcription transcription NN cord-275664-qbafkxtr 146 11 factor factor NN cord-275664-qbafkxtr 146 12 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 146 13 , , , cord-275664-qbafkxtr 146 14 which which WDT cord-275664-qbafkxtr 146 15 is be VBZ cord-275664-qbafkxtr 146 16 a a DT cord-275664-qbafkxtr 146 17 downstream downstream JJ cord-275664-qbafkxtr 146 18 substrate substrate NN cord-275664-qbafkxtr 146 19 of of IN cord-275664-qbafkxtr 146 20 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 146 21 , , , cord-275664-qbafkxtr 146 22 with with IN cord-275664-qbafkxtr 146 23 kinetics kinetic NNS cord-275664-qbafkxtr 146 24 that that WDT cord-275664-qbafkxtr 146 25 paralleled parallel VBD cord-275664-qbafkxtr 146 26 those those DT cord-275664-qbafkxtr 146 27 observed observe VBN cord-275664-qbafkxtr 146 28 for for IN cord-275664-qbafkxtr 146 29 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 147 1 ( ( -LRB- cord-275664-qbafkxtr 147 2 Fig Fig NNP cord-275664-qbafkxtr 147 3 . . . cord-275664-qbafkxtr 147 4 3 3 CD cord-275664-qbafkxtr 147 5 ) ) -RRB- cord-275664-qbafkxtr 147 6 . . . cord-275664-qbafkxtr 148 1 PCV2 pcv2 JJ cord-275664-qbafkxtr 148 2 replication replication NN cord-275664-qbafkxtr 148 3 was be VBD cord-275664-qbafkxtr 148 4 inhibited inhibit VBN cord-275664-qbafkxtr 148 5 with with IN cord-275664-qbafkxtr 148 6 the the DT cord-275664-qbafkxtr 148 7 MEK1/2 MEK1/2 NNP cord-275664-qbafkxtr 148 8 U0126 U0126 NNP cord-275664-qbafkxtr 148 9 ( ( -LRB- cord-275664-qbafkxtr 148 10 Fig fig NN cord-275664-qbafkxtr 148 11 . . NNP cord-275664-qbafkxtr 148 12 4 4 CD cord-275664-qbafkxtr 148 13 ) ) -RRB- cord-275664-qbafkxtr 148 14 . . . cord-275664-qbafkxtr 149 1 U0126 U0126 NNP cord-275664-qbafkxtr 149 2 inhibited inhibit VBN cord-275664-qbafkxtr 149 3 viral viral JJ cord-275664-qbafkxtr 149 4 replication replication NN cord-275664-qbafkxtr 149 5 by by IN cord-275664-qbafkxtr 149 6 28 28 CD cord-275664-qbafkxtr 149 7 % % NN cord-275664-qbafkxtr 149 8 when when WRB cord-275664-qbafkxtr 149 9 added add VBD cord-275664-qbafkxtr 149 10 up up RP cord-275664-qbafkxtr 149 11 to to TO cord-275664-qbafkxtr 149 12 8 8 CD cord-275664-qbafkxtr 149 13 h h NN cord-275664-qbafkxtr 149 14 postinfection postinfection NN cord-275664-qbafkxtr 149 15 ( ( -LRB- cord-275664-qbafkxtr 149 16 Fig Fig NNP cord-275664-qbafkxtr 149 17 . . NNP cord-275664-qbafkxtr 149 18 5 5 CD cord-275664-qbafkxtr 149 19 ) ) -RRB- cord-275664-qbafkxtr 149 20 , , , cord-275664-qbafkxtr 149 21 but but CC cord-275664-qbafkxtr 149 22 subsequent subsequent JJ cord-275664-qbafkxtr 149 23 addition addition NN cord-275664-qbafkxtr 149 24 had have VBD cord-275664-qbafkxtr 149 25 little little JJ cord-275664-qbafkxtr 149 26 effect effect NN cord-275664-qbafkxtr 149 27 , , , cord-275664-qbafkxtr 149 28 confirming confirm VBG cord-275664-qbafkxtr 149 29 that that IN cord-275664-qbafkxtr 149 30 ERK ERK NNP cord-275664-qbafkxtr 149 31 is be VBZ cord-275664-qbafkxtr 149 32 important important JJ cord-275664-qbafkxtr 149 33 at at IN cord-275664-qbafkxtr 149 34 early early JJ cord-275664-qbafkxtr 149 35 steps step NNS cord-275664-qbafkxtr 149 36 of of IN cord-275664-qbafkxtr 149 37 the the DT cord-275664-qbafkxtr 149 38 viral viral JJ cord-275664-qbafkxtr 149 39 cycle cycle NN cord-275664-qbafkxtr 149 40 . . . cord-275664-qbafkxtr 150 1 In in IN cord-275664-qbafkxtr 150 2 addition addition NN cord-275664-qbafkxtr 150 3 , , , cord-275664-qbafkxtr 150 4 PCV2 pcv2 JJ cord-275664-qbafkxtr 150 5 viral viral JJ cord-275664-qbafkxtr 150 6 transcription transcription NN cord-275664-qbafkxtr 150 7 ( ( -LRB- cord-275664-qbafkxtr 150 8 Fig Fig NNP cord-275664-qbafkxtr 150 9 . . . cord-275664-qbafkxtr 150 10 6B 6b CD cord-275664-qbafkxtr 150 11 ) ) -RRB- cord-275664-qbafkxtr 150 12 and and CC cord-275664-qbafkxtr 150 13 virus virus NN cord-275664-qbafkxtr 150 14 protein protein NN cord-275664-qbafkxtr 150 15 synthesis synthesis NN cord-275664-qbafkxtr 150 16 ( ( -LRB- cord-275664-qbafkxtr 150 17 Fig Fig NNP cord-275664-qbafkxtr 150 18 . . . cord-275664-qbafkxtr 150 19 6A 6a CD cord-275664-qbafkxtr 150 20 ) ) -RRB- cord-275664-qbafkxtr 150 21 were be VBD cord-275664-qbafkxtr 150 22 reduced reduce VBN cord-275664-qbafkxtr 150 23 in in IN cord-275664-qbafkxtr 150 24 the the DT cord-275664-qbafkxtr 150 25 presence presence NN cord-275664-qbafkxtr 150 26 of of IN cord-275664-qbafkxtr 150 27 U0126 U0126 NNP cord-275664-qbafkxtr 150 28 . . . cord-275664-qbafkxtr 151 1 Together together RB cord-275664-qbafkxtr 151 2 , , , cord-275664-qbafkxtr 151 3 these these DT cord-275664-qbafkxtr 151 4 data datum NNS cord-275664-qbafkxtr 151 5 suggest suggest VBP cord-275664-qbafkxtr 151 6 that that IN cord-275664-qbafkxtr 151 7 the the DT cord-275664-qbafkxtr 151 8 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 151 9 signaling signal VBG cord-275664-qbafkxtr 151 10 pathway pathway NN cord-275664-qbafkxtr 151 11 is be VBZ cord-275664-qbafkxtr 151 12 manipulated manipulate VBN cord-275664-qbafkxtr 151 13 by by IN cord-275664-qbafkxtr 151 14 PCV2 PCV2 NNP cord-275664-qbafkxtr 151 15 and and CC cord-275664-qbafkxtr 151 16 that that IN cord-275664-qbafkxtr 151 17 the the DT cord-275664-qbafkxtr 151 18 pathway pathway NN cord-275664-qbafkxtr 151 19 plays play VBZ cord-275664-qbafkxtr 151 20 a a DT cord-275664-qbafkxtr 151 21 beneficial beneficial JJ cord-275664-qbafkxtr 151 22 role role NN cord-275664-qbafkxtr 151 23 in in IN cord-275664-qbafkxtr 151 24 PCV2 PCV2 NNP cord-275664-qbafkxtr 151 25 replication replication NN cord-275664-qbafkxtr 151 26 . . . cord-275664-qbafkxtr 152 1 Activation activation NN cord-275664-qbafkxtr 152 2 of of IN cord-275664-qbafkxtr 152 3 the the DT cord-275664-qbafkxtr 152 4 ERK ERK NNP cord-275664-qbafkxtr 152 5 pathway pathway NN cord-275664-qbafkxtr 152 6 seems seem VBZ cord-275664-qbafkxtr 152 7 to to TO cord-275664-qbafkxtr 152 8 be be VB cord-275664-qbafkxtr 152 9 an an DT cord-275664-qbafkxtr 152 10 essential essential JJ cord-275664-qbafkxtr 152 11 requirement requirement NN cord-275664-qbafkxtr 152 12 to to TO cord-275664-qbafkxtr 152 13 regulate regulate VB cord-275664-qbafkxtr 152 14 signals signal NNS cord-275664-qbafkxtr 152 15 associated associate VBN cord-275664-qbafkxtr 152 16 with with IN cord-275664-qbafkxtr 152 17 biological biological JJ cord-275664-qbafkxtr 152 18 functions function NNS cord-275664-qbafkxtr 152 19 during during IN cord-275664-qbafkxtr 152 20 virus virus NN cord-275664-qbafkxtr 152 21 infections infection NNS cord-275664-qbafkxtr 152 22 . . . cord-275664-qbafkxtr 153 1 Many many JJ cord-275664-qbafkxtr 153 2 viruses virus NNS cord-275664-qbafkxtr 153 3 utilize utilize VBP cord-275664-qbafkxtr 153 4 the the DT cord-275664-qbafkxtr 153 5 ERK ERK NNP cord-275664-qbafkxtr 153 6 pathway pathway NN cord-275664-qbafkxtr 153 7 for for IN cord-275664-qbafkxtr 153 8 maximal maximal JJ cord-275664-qbafkxtr 153 9 viral viral JJ cord-275664-qbafkxtr 153 10 replication replication NN cord-275664-qbafkxtr 153 11 . . . cord-275664-qbafkxtr 154 1 Activated activate VBN cord-275664-qbafkxtr 154 2 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 154 3 was be VBD cord-275664-qbafkxtr 154 4 reported report VBN cord-275664-qbafkxtr 154 5 to to TO cord-275664-qbafkxtr 154 6 enhance enhance VB cord-275664-qbafkxtr 154 7 the the DT cord-275664-qbafkxtr 154 8 infectivity infectivity NN cord-275664-qbafkxtr 154 9 of of IN cord-275664-qbafkxtr 154 10 human human JJ cord-275664-qbafkxtr 154 11 immunodeficiency immunodeficiency NN cord-275664-qbafkxtr 154 12 virus virus NN cord-275664-qbafkxtr 154 13 ( ( -LRB- cord-275664-qbafkxtr 154 14 HIV HIV NNP cord-275664-qbafkxtr 154 15 ) ) -RRB- cord-275664-qbafkxtr 154 16 , , , cord-275664-qbafkxtr 154 17 whereas whereas IN cord-275664-qbafkxtr 154 18 treatment treatment NN cord-275664-qbafkxtr 154 19 of of IN cord-275664-qbafkxtr 154 20 cells cell NNS cord-275664-qbafkxtr 154 21 with with IN cord-275664-qbafkxtr 154 22 the the DT cord-275664-qbafkxtr 154 23 MEK1/2 MEK1/2 NNP cord-275664-qbafkxtr 154 24 inhibitor inhibitor NN cord-275664-qbafkxtr 154 25 significantly significantly RB cord-275664-qbafkxtr 154 26 inhibited inhibit VBD cord-275664-qbafkxtr 154 27 HIV HIV NNP cord-275664-qbafkxtr 154 28 infectivity infectivity NN cord-275664-qbafkxtr 154 29 Gabuzda Gabuzda NNP cord-275664-qbafkxtr 154 30 , , , cord-275664-qbafkxtr 154 31 1998 1998 CD cord-275664-qbafkxtr 154 32 , , , cord-275664-qbafkxtr 154 33 1999 1999 CD cord-275664-qbafkxtr 154 34 ) ) -RRB- cord-275664-qbafkxtr 154 35 . . . cord-275664-qbafkxtr 155 1 ERK ERK NNP cord-275664-qbafkxtr 155 2 MAPK MAPK NNP cord-275664-qbafkxtr 155 3 is be VBZ cord-275664-qbafkxtr 155 4 required require VBN cord-275664-qbafkxtr 155 5 for for IN cord-275664-qbafkxtr 155 6 Visna Visna NNP cord-275664-qbafkxtr 155 7 virus virus NN cord-275664-qbafkxtr 155 8 replication replication NN cord-275664-qbafkxtr 155 9 and and CC cord-275664-qbafkxtr 155 10 virus virus NN cord-275664-qbafkxtr 155 11 - - HYPH cord-275664-qbafkxtr 155 12 induced induce VBN cord-275664-qbafkxtr 155 13 neuropathology neuropathology NN cord-275664-qbafkxtr 155 14 ( ( -LRB- cord-275664-qbafkxtr 155 15 Barber Barber NNP cord-275664-qbafkxtr 155 16 et et NNP cord-275664-qbafkxtr 155 17 al al NNP cord-275664-qbafkxtr 155 18 . . NNP cord-275664-qbafkxtr 155 19 , , , cord-275664-qbafkxtr 155 20 2002 2002 CD cord-275664-qbafkxtr 155 21 ) ) -RRB- cord-275664-qbafkxtr 155 22 . . . cord-275664-qbafkxtr 156 1 Treating treat VBG cord-275664-qbafkxtr 156 2 cells cell NNS cord-275664-qbafkxtr 156 3 with with IN cord-275664-qbafkxtr 156 4 the the DT cord-275664-qbafkxtr 156 5 MEK1/2 MEK1/2 NNP cord-275664-qbafkxtr 156 6 inhibitor inhibitor NN cord-275664-qbafkxtr 156 7 also also RB cord-275664-qbafkxtr 156 8 significantly significantly RB cord-275664-qbafkxtr 156 9 inhibited inhibit VBD cord-275664-qbafkxtr 156 10 the the DT cord-275664-qbafkxtr 156 11 propagation propagation NN cord-275664-qbafkxtr 156 12 of of IN cord-275664-qbafkxtr 156 13 influenza influenza NN cord-275664-qbafkxtr 156 14 A a DT cord-275664-qbafkxtr 156 15 virus virus NN cord-275664-qbafkxtr 156 16 , , , cord-275664-qbafkxtr 156 17 Borna Borna NNP cord-275664-qbafkxtr 156 18 disease disease NN cord-275664-qbafkxtr 156 19 virus virus NN cord-275664-qbafkxtr 156 20 , , , cord-275664-qbafkxtr 156 21 Coxsackievirus Coxsackievirus NNP cord-275664-qbafkxtr 156 22 B3 b3 NN cord-275664-qbafkxtr 156 23 ( ( -LRB- cord-275664-qbafkxtr 156 24 Luo Luo NNP cord-275664-qbafkxtr 156 25 et et NNP cord-275664-qbafkxtr 156 26 al al NNP cord-275664-qbafkxtr 156 27 . . NNP cord-275664-qbafkxtr 156 28 , , , cord-275664-qbafkxtr 156 29 2002 2002 CD cord-275664-qbafkxtr 156 30 ) ) -RRB- cord-275664-qbafkxtr 156 31 , , , cord-275664-qbafkxtr 156 32 human human JJ cord-275664-qbafkxtr 156 33 cytomegalovirus cytomegalovirus NN cord-275664-qbafkxtr 156 34 ( ( -LRB- cord-275664-qbafkxtr 156 35 Johnson Johnson NNP cord-275664-qbafkxtr 156 36 et et NNP cord-275664-qbafkxtr 156 37 al al NNP cord-275664-qbafkxtr 156 38 . . NNP cord-275664-qbafkxtr 156 39 , , , cord-275664-qbafkxtr 156 40 2001 2001 CD cord-275664-qbafkxtr 156 41 ) ) -RRB- cord-275664-qbafkxtr 156 42 , , , cord-275664-qbafkxtr 156 43 vaccinia vaccinia NN cord-275664-qbafkxtr 156 44 virus virus NN cord-275664-qbafkxtr 156 45 ( ( -LRB- cord-275664-qbafkxtr 156 46 Andrade Andrade NNP cord-275664-qbafkxtr 156 47 et et NNP cord-275664-qbafkxtr 156 48 al al NNP cord-275664-qbafkxtr 156 49 . . NNP cord-275664-qbafkxtr 156 50 , , , cord-275664-qbafkxtr 156 51 2004 2004 CD cord-275664-qbafkxtr 156 52 ) ) -RRB- cord-275664-qbafkxtr 156 53 , , , cord-275664-qbafkxtr 156 54 coronavirus coronavirus NN cord-275664-qbafkxtr 156 55 ( ( -LRB- cord-275664-qbafkxtr 156 56 Cai Cai NNP cord-275664-qbafkxtr 156 57 et et FW cord-275664-qbafkxtr 156 58 al al NNP cord-275664-qbafkxtr 156 59 . . NNP cord-275664-qbafkxtr 156 60 , , , cord-275664-qbafkxtr 156 61 2007 2007 CD cord-275664-qbafkxtr 156 62 ) ) -RRB- cord-275664-qbafkxtr 156 63 , , , cord-275664-qbafkxtr 156 64 and and CC cord-275664-qbafkxtr 156 65 astrovirus astrovirus NNP cord-275664-qbafkxtr 156 66 ( ( -LRB- cord-275664-qbafkxtr 156 67 Moser Moser NNP cord-275664-qbafkxtr 156 68 and and CC cord-275664-qbafkxtr 156 69 Schultz Schultz NNP cord-275664-qbafkxtr 156 70 - - HYPH cord-275664-qbafkxtr 156 71 Cherry Cherry NNP cord-275664-qbafkxtr 156 72 , , , cord-275664-qbafkxtr 156 73 2008 2008 CD cord-275664-qbafkxtr 156 74 ) ) -RRB- cord-275664-qbafkxtr 156 75 . . . cord-275664-qbafkxtr 157 1 In in IN cord-275664-qbafkxtr 157 2 the the DT cord-275664-qbafkxtr 157 3 present present JJ cord-275664-qbafkxtr 157 4 study study NN cord-275664-qbafkxtr 157 5 , , , cord-275664-qbafkxtr 157 6 we -PRON- PRP cord-275664-qbafkxtr 157 7 determined determine VBD cord-275664-qbafkxtr 157 8 whether whether IN cord-275664-qbafkxtr 157 9 PCV2-induced PCV2-induced NNP cord-275664-qbafkxtr 157 10 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 157 11 activation activation NN cord-275664-qbafkxtr 157 12 is be VBZ cord-275664-qbafkxtr 157 13 involved involve VBN cord-275664-qbafkxtr 157 14 in in IN cord-275664-qbafkxtr 157 15 viral viral JJ cord-275664-qbafkxtr 157 16 replication replication NN cord-275664-qbafkxtr 157 17 by by IN cord-275664-qbafkxtr 157 18 examining examine VBG cord-275664-qbafkxtr 157 19 the the DT cord-275664-qbafkxtr 157 20 effect effect NN cord-275664-qbafkxtr 157 21 of of IN cord-275664-qbafkxtr 157 22 the the DT cord-275664-qbafkxtr 157 23 inhibitor inhibitor NN cord-275664-qbafkxtr 157 24 U0126 U0126 NNP cord-275664-qbafkxtr 157 25 on on IN cord-275664-qbafkxtr 157 26 PCV2 PCV2 NNP cord-275664-qbafkxtr 157 27 production production NN cord-275664-qbafkxtr 157 28 and and CC cord-275664-qbafkxtr 157 29 found find VBD cord-275664-qbafkxtr 157 30 that that DT cord-275664-qbafkxtr 157 31 inhibition inhibition NN cord-275664-qbafkxtr 157 32 of of IN cord-275664-qbafkxtr 157 33 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 157 34 activation activation NN cord-275664-qbafkxtr 157 35 significantly significantly RB cord-275664-qbafkxtr 157 36 reduced reduce VBD cord-275664-qbafkxtr 157 37 the the DT cord-275664-qbafkxtr 157 38 production production NN cord-275664-qbafkxtr 157 39 of of IN cord-275664-qbafkxtr 157 40 progeny progeny JJ cord-275664-qbafkxtr 157 41 virus virus NN cord-275664-qbafkxtr 157 42 ( ( -LRB- cord-275664-qbafkxtr 157 43 Fig Fig NNP cord-275664-qbafkxtr 157 44 . . . cord-275664-qbafkxtr 157 45 4 4 CD cord-275664-qbafkxtr 157 46 ) ) -RRB- cord-275664-qbafkxtr 157 47 . . . cord-275664-qbafkxtr 158 1 This this DT cord-275664-qbafkxtr 158 2 shows show VBZ cord-275664-qbafkxtr 158 3 that that DT cord-275664-qbafkxtr 158 4 optimal optimal JJ cord-275664-qbafkxtr 159 1 PCV2 pcv2 JJ cord-275664-qbafkxtr 159 2 replication replication NN cord-275664-qbafkxtr 159 3 requires require VBZ cord-275664-qbafkxtr 159 4 the the DT cord-275664-qbafkxtr 159 5 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 159 6 activity activity NN cord-275664-qbafkxtr 159 7 , , , cord-275664-qbafkxtr 159 8 and and CC cord-275664-qbafkxtr 159 9 raises raise VBZ cord-275664-qbafkxtr 159 10 the the DT cord-275664-qbafkxtr 159 11 possibility possibility NN cord-275664-qbafkxtr 159 12 that that IN cord-275664-qbafkxtr 159 13 PCV2 PCV2 NNP cord-275664-qbafkxtr 159 14 has have VBZ cord-275664-qbafkxtr 159 15 acquired acquire VBN cord-275664-qbafkxtr 159 16 the the DT cord-275664-qbafkxtr 159 17 ability ability NN cord-275664-qbafkxtr 159 18 to to TO cord-275664-qbafkxtr 159 19 activate activate VB cord-275664-qbafkxtr 159 20 the the DT cord-275664-qbafkxtr 159 21 kinase kinase NN cord-275664-qbafkxtr 159 22 to to TO cord-275664-qbafkxtr 159 23 aid aid VB cord-275664-qbafkxtr 159 24 its -PRON- PRP$ cord-275664-qbafkxtr 159 25 replication replication NN cord-275664-qbafkxtr 159 26 . . . cord-275664-qbafkxtr 160 1 Therefore therefore RB cord-275664-qbafkxtr 160 2 , , , cord-275664-qbafkxtr 160 3 our -PRON- PRP$ cord-275664-qbafkxtr 160 4 findings finding NNS cord-275664-qbafkxtr 160 5 indicate indicate VBP cord-275664-qbafkxtr 160 6 that that IN cord-275664-qbafkxtr 160 7 PCV2-induced pcv2-induced JJ cord-275664-qbafkxtr 160 8 activation activation NN cord-275664-qbafkxtr 160 9 of of IN cord-275664-qbafkxtr 160 10 ERK ERK NNP cord-275664-qbafkxtr 160 11 is be VBZ cord-275664-qbafkxtr 160 12 truly truly RB cord-275664-qbafkxtr 160 13 involved involve VBN cord-275664-qbafkxtr 160 14 in in IN cord-275664-qbafkxtr 160 15 the the DT cord-275664-qbafkxtr 160 16 replication replication NN cord-275664-qbafkxtr 160 17 of of IN cord-275664-qbafkxtr 160 18 PCV2 PCV2 NNP cord-275664-qbafkxtr 160 19 in in IN cord-275664-qbafkxtr 160 20 PK15 PK15 NNP cord-275664-qbafkxtr 160 21 cells cell NNS cord-275664-qbafkxtr 160 22 and and CC cord-275664-qbafkxtr 160 23 add add VB cord-275664-qbafkxtr 160 24 a a DT cord-275664-qbafkxtr 160 25 new new JJ cord-275664-qbafkxtr 160 26 member member NN cord-275664-qbafkxtr 160 27 to to IN cord-275664-qbafkxtr 160 28 the the DT cord-275664-qbafkxtr 160 29 growing grow VBG cord-275664-qbafkxtr 160 30 list list NN cord-275664-qbafkxtr 160 31 of of IN cord-275664-qbafkxtr 160 32 viruses virus NNS cord-275664-qbafkxtr 160 33 whose whose WP$ cord-275664-qbafkxtr 160 34 replication replication NN cord-275664-qbafkxtr 160 35 is be VBZ cord-275664-qbafkxtr 160 36 modulated modulate VBN cord-275664-qbafkxtr 160 37 by by IN cord-275664-qbafkxtr 160 38 the the DT cord-275664-qbafkxtr 160 39 ERK ERK NNP cord-275664-qbafkxtr 160 40 signaling signal VBG cord-275664-qbafkxtr 160 41 pathway pathway NN cord-275664-qbafkxtr 160 42 . . . cord-275664-qbafkxtr 161 1 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 162 1 regulation regulation NN cord-275664-qbafkxtr 162 2 of of IN cord-275664-qbafkxtr 162 3 viral viral JJ cord-275664-qbafkxtr 162 4 replication replication NN cord-275664-qbafkxtr 162 5 can can MD cord-275664-qbafkxtr 162 6 act act VB cord-275664-qbafkxtr 162 7 at at IN cord-275664-qbafkxtr 162 8 specific specific JJ cord-275664-qbafkxtr 162 9 steps step NNS cord-275664-qbafkxtr 162 10 of of IN cord-275664-qbafkxtr 162 11 the the DT cord-275664-qbafkxtr 162 12 replication replication NN cord-275664-qbafkxtr 162 13 cycle cycle NN cord-275664-qbafkxtr 162 14 , , , cord-275664-qbafkxtr 162 15 such such JJ cord-275664-qbafkxtr 162 16 as as IN cord-275664-qbafkxtr 162 17 attachment attachment NN cord-275664-qbafkxtr 162 18 , , , cord-275664-qbafkxtr 162 19 entry entry NN cord-275664-qbafkxtr 162 20 , , , cord-275664-qbafkxtr 162 21 gene gene NN cord-275664-qbafkxtr 162 22 transcription transcription NN cord-275664-qbafkxtr 162 23 , , , cord-275664-qbafkxtr 162 24 protein protein NN cord-275664-qbafkxtr 162 25 expression expression NN cord-275664-qbafkxtr 162 26 , , , cord-275664-qbafkxtr 162 27 and and CC cord-275664-qbafkxtr 162 28 assembly assembly NN cord-275664-qbafkxtr 162 29 . . . cord-275664-qbafkxtr 163 1 Borna Borna NNP cord-275664-qbafkxtr 163 2 disease disease NN cord-275664-qbafkxtr 163 3 virus virus NN cord-275664-qbafkxtr 163 4 requires require VBZ cord-275664-qbafkxtr 163 5 active active JJ cord-275664-qbafkxtr 163 6 ERK ERK NNP cord-275664-qbafkxtr 163 7 for for IN cord-275664-qbafkxtr 163 8 entry entry NN cord-275664-qbafkxtr 163 9 . . . cord-275664-qbafkxtr 164 1 HIV-1 HIV-1 NNP cord-275664-qbafkxtr 164 2 depends depend VBZ cord-275664-qbafkxtr 164 3 on on IN cord-275664-qbafkxtr 164 4 the the DT cord-275664-qbafkxtr 164 5 activation activation NN cord-275664-qbafkxtr 164 6 of of IN cord-275664-qbafkxtr 164 7 MEK MEK NNP cord-275664-qbafkxtr 164 8 ERK ERK NNP cord-275664-qbafkxtr 164 9 pathway pathway NN cord-275664-qbafkxtr 164 10 to to TO cord-275664-qbafkxtr 164 11 deliver deliver VB cord-275664-qbafkxtr 164 12 its -PRON- PRP$ cord-275664-qbafkxtr 164 13 genomes genome NNS cord-275664-qbafkxtr 164 14 into into IN cord-275664-qbafkxtr 164 15 the the DT cord-275664-qbafkxtr 164 16 cultured cultured JJ cord-275664-qbafkxtr 164 17 cells cell NNS cord-275664-qbafkxtr 164 18 ( ( -LRB- cord-275664-qbafkxtr 164 19 Liu Liu NNP cord-275664-qbafkxtr 164 20 et et NNP cord-275664-qbafkxtr 164 21 al al NNP cord-275664-qbafkxtr 164 22 . . NNP cord-275664-qbafkxtr 164 23 , , , cord-275664-qbafkxtr 164 24 2002 2002 CD cord-275664-qbafkxtr 164 25 ) ) -RRB- cord-275664-qbafkxtr 164 26 . . . cord-275664-qbafkxtr 165 1 Inhibition inhibition NN cord-275664-qbafkxtr 165 2 of of IN cord-275664-qbafkxtr 165 3 ERK ERK NNP cord-275664-qbafkxtr 165 4 during during IN cord-275664-qbafkxtr 165 5 coronavirus coronavirus NN cord-275664-qbafkxtr 165 6 infection infection NN cord-275664-qbafkxtr 165 7 specifically specifically RB cord-275664-qbafkxtr 165 8 decreases decrease VBZ cord-275664-qbafkxtr 165 9 genomic genomic JJ cord-275664-qbafkxtr 165 10 and and CC cord-275664-qbafkxtr 165 11 sgRNA sgRNA JJR cord-275664-qbafkxtr 165 12 production production NN cord-275664-qbafkxtr 165 13 but but CC cord-275664-qbafkxtr 165 14 has have VBZ cord-275664-qbafkxtr 165 15 no no DT cord-275664-qbafkxtr 165 16 effect effect NN cord-275664-qbafkxtr 165 17 on on IN cord-275664-qbafkxtr 165 18 protein protein NN cord-275664-qbafkxtr 165 19 synthesis synthesis NN cord-275664-qbafkxtr 165 20 ( ( -LRB- cord-275664-qbafkxtr 166 1 Cai Cai NNP cord-275664-qbafkxtr 166 2 et et FW cord-275664-qbafkxtr 166 3 al al NNP cord-275664-qbafkxtr 166 4 . . NNP cord-275664-qbafkxtr 166 5 , , , cord-275664-qbafkxtr 166 6 2007 2007 CD cord-275664-qbafkxtr 166 7 ) ) -RRB- cord-275664-qbafkxtr 166 8 . . . cord-275664-qbafkxtr 167 1 ERK ERK NNP cord-275664-qbafkxtr 167 2 activates activate VBZ cord-275664-qbafkxtr 167 3 adenovirus adenovirus NN cord-275664-qbafkxtr 167 4 gene gene NN cord-275664-qbafkxtr 167 5 transactivators transactivator NNS cord-275664-qbafkxtr 167 6 , , , cord-275664-qbafkxtr 167 7 modulating modulate VBG cord-275664-qbafkxtr 167 8 protein protein NN cord-275664-qbafkxtr 167 9 expression expression NN cord-275664-qbafkxtr 167 10 ( ( -LRB- cord-275664-qbafkxtr 167 11 Schümann Schümann NNP cord-275664-qbafkxtr 167 12 and and CC cord-275664-qbafkxtr 167 13 Dobbelstein Dobbelstein NNP cord-275664-qbafkxtr 167 14 , , , cord-275664-qbafkxtr 167 15 2006 2006 CD cord-275664-qbafkxtr 167 16 ; ; : cord-275664-qbafkxtr 167 17 Whalen Whalen NNP cord-275664-qbafkxtr 167 18 et et NNP cord-275664-qbafkxtr 167 19 al al NNP cord-275664-qbafkxtr 167 20 . . NNP cord-275664-qbafkxtr 167 21 , , , cord-275664-qbafkxtr 167 22 1997 1997 CD cord-275664-qbafkxtr 167 23 ) ) -RRB- cord-275664-qbafkxtr 167 24 , , , cord-275664-qbafkxtr 167 25 with with IN cord-275664-qbafkxtr 167 26 little little JJ cord-275664-qbafkxtr 167 27 effect effect NN cord-275664-qbafkxtr 167 28 on on IN cord-275664-qbafkxtr 167 29 RNA RNA NNP cord-275664-qbafkxtr 167 30 levels level NNS cord-275664-qbafkxtr 167 31 ( ( -LRB- cord-275664-qbafkxtr 167 32 Schümann Schümann NNP cord-275664-qbafkxtr 167 33 and and CC cord-275664-qbafkxtr 167 34 Dobbelstein Dobbelstein NNP cord-275664-qbafkxtr 167 35 , , , cord-275664-qbafkxtr 167 36 2006 2006 CD cord-275664-qbafkxtr 167 37 ) ) -RRB- cord-275664-qbafkxtr 167 38 . . . cord-275664-qbafkxtr 168 1 Inhibition inhibition NN cord-275664-qbafkxtr 168 2 of of IN cord-275664-qbafkxtr 168 3 MEK MEK NNP cord-275664-qbafkxtr 168 4 / / SYM cord-275664-qbafkxtr 168 5 ERK ERK NNP cord-275664-qbafkxtr 168 6 pathway pathway NN cord-275664-qbafkxtr 168 7 reduces reduce VBZ cord-275664-qbafkxtr 168 8 Kaposi Kaposi NNP cord-275664-qbafkxtr 168 9 's 's POS cord-275664-qbafkxtr 168 10 sarcoma sarcoma NN cord-275664-qbafkxtr 168 11 - - HYPH cord-275664-qbafkxtr 168 12 associated associate VBN cord-275664-qbafkxtr 168 13 herpesvirus herpesvirus NN cord-275664-qbafkxtr 168 14 infectivity infectivity NN cord-275664-qbafkxtr 168 15 but but CC cord-275664-qbafkxtr 168 16 has have VBZ cord-275664-qbafkxtr 168 17 no no DT cord-275664-qbafkxtr 168 18 effect effect NN cord-275664-qbafkxtr 168 19 on on IN cord-275664-qbafkxtr 168 20 virus virus NN cord-275664-qbafkxtr 168 21 binding bind VBG cord-275664-qbafkxtr 168 22 to to IN cord-275664-qbafkxtr 168 23 the the DT cord-275664-qbafkxtr 168 24 cell cell NN cord-275664-qbafkxtr 168 25 surface surface NN cord-275664-qbafkxtr 168 26 receptor receptor NN cord-275664-qbafkxtr 168 27 ( ( -LRB- cord-275664-qbafkxtr 168 28 Naranatt Naranatt NNP cord-275664-qbafkxtr 168 29 et et FW cord-275664-qbafkxtr 168 30 al al NNP cord-275664-qbafkxtr 168 31 . . NNP cord-275664-qbafkxtr 168 32 , , , cord-275664-qbafkxtr 168 33 2003 2003 CD cord-275664-qbafkxtr 168 34 ) ) -RRB- cord-275664-qbafkxtr 168 35 . . . cord-275664-qbafkxtr 169 1 Vaccinia vaccinia NN cord-275664-qbafkxtr 169 2 virus virus NN cord-275664-qbafkxtr 169 3 - - HYPH cord-275664-qbafkxtr 169 4 induced induce VBN cord-275664-qbafkxtr 169 5 ERK ERK NNP cord-275664-qbafkxtr 169 6 activation activation NN cord-275664-qbafkxtr 169 7 was be VBD cord-275664-qbafkxtr 169 8 significantly significantly RB cord-275664-qbafkxtr 169 9 blocked block VBN cord-275664-qbafkxtr 169 10 by by IN cord-275664-qbafkxtr 169 11 inhibition inhibition NN cord-275664-qbafkxtr 169 12 of of IN cord-275664-qbafkxtr 169 13 ERK ERK NNP cord-275664-qbafkxtr 169 14 , , , cord-275664-qbafkxtr 169 15 which which WDT cord-275664-qbafkxtr 169 16 was be VBD cord-275664-qbafkxtr 169 17 simultaneously simultaneously RB cord-275664-qbafkxtr 169 18 paralleled parallel VBN cord-275664-qbafkxtr 169 19 by by IN cord-275664-qbafkxtr 169 20 both both CC cord-275664-qbafkxtr 169 21 delayed delay VBN cord-275664-qbafkxtr 169 22 viral viral JJ cord-275664-qbafkxtr 169 23 early early JJ cord-275664-qbafkxtr 169 24 - - HYPH cord-275664-qbafkxtr 169 25 gene gene NN cord-275664-qbafkxtr 169 26 expression expression NN cord-275664-qbafkxtr 169 27 and and CC cord-275664-qbafkxtr 169 28 a a DT cord-275664-qbafkxtr 169 29 decrease decrease NN cord-275664-qbafkxtr 169 30 in in IN cord-275664-qbafkxtr 169 31 viral viral JJ cord-275664-qbafkxtr 169 32 DNA dna NN cord-275664-qbafkxtr 169 33 synthesis synthesis NN cord-275664-qbafkxtr 169 34 ( ( -LRB- cord-275664-qbafkxtr 169 35 Andrade Andrade NNP cord-275664-qbafkxtr 169 36 et et NNP cord-275664-qbafkxtr 169 37 al al NNP cord-275664-qbafkxtr 169 38 . . NNP cord-275664-qbafkxtr 169 39 , , , cord-275664-qbafkxtr 169 40 2004 2004 CD cord-275664-qbafkxtr 169 41 ) ) -RRB- cord-275664-qbafkxtr 169 42 . . . cord-275664-qbafkxtr 170 1 Inhibition inhibition NN cord-275664-qbafkxtr 170 2 of of IN cord-275664-qbafkxtr 170 3 ERK ERK NNP cord-275664-qbafkxtr 170 4 activation activation NN cord-275664-qbafkxtr 170 5 mediated mediate VBN cord-275664-qbafkxtr 170 6 by by IN cord-275664-qbafkxtr 170 7 astrovirus astrovirus NNP cord-275664-qbafkxtr 170 8 reduces reduce VBZ cord-275664-qbafkxtr 170 9 all all DT cord-275664-qbafkxtr 170 10 steps step NNS cord-275664-qbafkxtr 170 11 of of IN cord-275664-qbafkxtr 170 12 the the DT cord-275664-qbafkxtr 170 13 viral viral JJ cord-275664-qbafkxtr 170 14 life life NN cord-275664-qbafkxtr 170 15 cycle cycle NN cord-275664-qbafkxtr 170 16 , , , cord-275664-qbafkxtr 170 17 including include VBG cord-275664-qbafkxtr 170 18 early early JJ cord-275664-qbafkxtr 170 19 and and CC cord-275664-qbafkxtr 170 20 late late JJ cord-275664-qbafkxtr 170 21 protein protein NN cord-275664-qbafkxtr 170 22 expression expression NN cord-275664-qbafkxtr 170 23 as as RB cord-275664-qbafkxtr 170 24 well well RB cord-275664-qbafkxtr 170 25 as as IN cord-275664-qbafkxtr 170 26 subgenomic subgenomic JJ cord-275664-qbafkxtr 170 27 and and CC cord-275664-qbafkxtr 170 28 genomic genomic JJ cord-275664-qbafkxtr 170 29 RNA RNA NNP cord-275664-qbafkxtr 170 30 transcription transcription NN cord-275664-qbafkxtr 170 31 ( ( -LRB- cord-275664-qbafkxtr 170 32 Moser Moser NNP cord-275664-qbafkxtr 170 33 and and CC cord-275664-qbafkxtr 170 34 Schultz Schultz NNP cord-275664-qbafkxtr 170 35 - - HYPH cord-275664-qbafkxtr 170 36 Cherry Cherry NNP cord-275664-qbafkxtr 170 37 , , , cord-275664-qbafkxtr 170 38 2008 2008 CD cord-275664-qbafkxtr 170 39 ) ) -RRB- cord-275664-qbafkxtr 170 40 . . . cord-275664-qbafkxtr 171 1 The the DT cord-275664-qbafkxtr 171 2 function function NN cord-275664-qbafkxtr 171 3 of of IN cord-275664-qbafkxtr 171 4 activated activate VBN cord-275664-qbafkxtr 171 5 ERK ERK NNP cord-275664-qbafkxtr 171 6 during during IN cord-275664-qbafkxtr 171 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 171 8 infection infection NN cord-275664-qbafkxtr 171 9 was be VBD cord-275664-qbafkxtr 171 10 investigated investigate VBN cord-275664-qbafkxtr 171 11 in in IN cord-275664-qbafkxtr 171 12 the the DT cord-275664-qbafkxtr 171 13 present present JJ cord-275664-qbafkxtr 171 14 study study NN cord-275664-qbafkxtr 171 15 . . . cord-275664-qbafkxtr 172 1 Our -PRON- PRP$ cord-275664-qbafkxtr 172 2 data datum NNS cord-275664-qbafkxtr 172 3 showed show VBD cord-275664-qbafkxtr 172 4 that that IN cord-275664-qbafkxtr 172 5 treatment treatment NN cord-275664-qbafkxtr 172 6 of of IN cord-275664-qbafkxtr 172 7 cells cell NNS cord-275664-qbafkxtr 172 8 with with IN cord-275664-qbafkxtr 172 9 U0126 U0126 NNP cord-275664-qbafkxtr 172 10 suppressed suppress VBN cord-275664-qbafkxtr 172 11 virus virus NN cord-275664-qbafkxtr 172 12 propagation propagation NN cord-275664-qbafkxtr 172 13 at at IN cord-275664-qbafkxtr 172 14 the the DT cord-275664-qbafkxtr 172 15 step step NN cord-275664-qbafkxtr 172 16 of of IN cord-275664-qbafkxtr 172 17 viral viral JJ cord-275664-qbafkxtr 172 18 RNA RNA NNP cord-275664-qbafkxtr 172 19 transcription transcription NN cord-275664-qbafkxtr 172 20 as as RB cord-275664-qbafkxtr 172 21 well well RB cord-275664-qbafkxtr 172 22 as as IN cord-275664-qbafkxtr 172 23 viral viral JJ cord-275664-qbafkxtr 172 24 protein protein NN cord-275664-qbafkxtr 172 25 synthesis synthesis NN cord-275664-qbafkxtr 172 26 . . . cord-275664-qbafkxtr 173 1 PCV PCV NNP cord-275664-qbafkxtr 173 2 replicates replicate VBZ cord-275664-qbafkxtr 173 3 via via IN cord-275664-qbafkxtr 173 4 rolling roll VBG cord-275664-qbafkxtr 173 5 circle circle NN cord-275664-qbafkxtr 173 6 replication replication NN cord-275664-qbafkxtr 173 7 involving involve VBG cord-275664-qbafkxtr 173 8 an an DT cord-275664-qbafkxtr 173 9 intermediate intermediate JJ cord-275664-qbafkxtr 173 10 double double RB cord-275664-qbafkxtr 173 11 - - HYPH cord-275664-qbafkxtr 173 12 stranded strand VBN cord-275664-qbafkxtr 173 13 replicative replicative JJ cord-275664-qbafkxtr 173 14 form form NN cord-275664-qbafkxtr 173 15 of of IN cord-275664-qbafkxtr 173 16 DNA dna NN cord-275664-qbafkxtr 173 17 , , , cord-275664-qbafkxtr 173 18 and and CC cord-275664-qbafkxtr 173 19 the the DT cord-275664-qbafkxtr 173 20 rep rep NNP cord-275664-qbafkxtr 173 21 proteins protein NNS cord-275664-qbafkxtr 173 22 nick nick NNP cord-275664-qbafkxtr 173 23 and and CC cord-275664-qbafkxtr 173 24 join join VB cord-275664-qbafkxtr 173 25 the the DT cord-275664-qbafkxtr 173 26 nucleotide nucleotide JJ cord-275664-qbafkxtr 173 27 segments segment NNS cord-275664-qbafkxtr 173 28 at at IN cord-275664-qbafkxtr 173 29 the the DT cord-275664-qbafkxtr 173 30 initiation initiation NN cord-275664-qbafkxtr 173 31 and and CC cord-275664-qbafkxtr 173 32 termination termination NN cord-275664-qbafkxtr 173 33 of of IN cord-275664-qbafkxtr 173 34 the the DT cord-275664-qbafkxtr 173 35 replication replication NN cord-275664-qbafkxtr 173 36 cycle cycle NN cord-275664-qbafkxtr 173 37 ( ( -LRB- cord-275664-qbafkxtr 173 38 Cheung Cheung NNP cord-275664-qbafkxtr 173 39 , , , cord-275664-qbafkxtr 173 40 2006 2006 CD cord-275664-qbafkxtr 173 41 ; ; : cord-275664-qbafkxtr 173 42 Steinfeldt Steinfeldt NNP cord-275664-qbafkxtr 173 43 et et NNP cord-275664-qbafkxtr 173 44 al al NNP cord-275664-qbafkxtr 173 45 . . NNP cord-275664-qbafkxtr 173 46 , , , cord-275664-qbafkxtr 173 47 2006 2006 CD cord-275664-qbafkxtr 173 48 ) ) -RRB- cord-275664-qbafkxtr 173 49 . . . cord-275664-qbafkxtr 174 1 Thus thus RB cord-275664-qbafkxtr 174 2 , , , cord-275664-qbafkxtr 174 3 it -PRON- PRP cord-275664-qbafkxtr 174 4 was be VBD cord-275664-qbafkxtr 174 5 not not RB cord-275664-qbafkxtr 174 6 surprising surprising JJ cord-275664-qbafkxtr 174 7 that that IN cord-275664-qbafkxtr 174 8 treatment treatment NN cord-275664-qbafkxtr 174 9 of of IN cord-275664-qbafkxtr 174 10 PCV2-infected pcv2-infected JJ cord-275664-qbafkxtr 174 11 cells cell NNS cord-275664-qbafkxtr 174 12 with with IN cord-275664-qbafkxtr 174 13 inhibition inhibition NN cord-275664-qbafkxtr 174 14 of of IN cord-275664-qbafkxtr 174 15 ERK ERK NNP cord-275664-qbafkxtr 174 16 reduced reduce VBN cord-275664-qbafkxtr 174 17 viral viral JJ cord-275664-qbafkxtr 174 18 transcripts transcript NNS cord-275664-qbafkxtr 174 19 as as RB cord-275664-qbafkxtr 174 20 well well RB cord-275664-qbafkxtr 174 21 as as IN cord-275664-qbafkxtr 174 22 protein protein NN cord-275664-qbafkxtr 174 23 expression expression NN cord-275664-qbafkxtr 174 24 , , , cord-275664-qbafkxtr 174 25 because because IN cord-275664-qbafkxtr 174 26 viral viral JJ cord-275664-qbafkxtr 174 27 transcription transcription NN cord-275664-qbafkxtr 174 28 and and CC cord-275664-qbafkxtr 174 29 protein protein NN cord-275664-qbafkxtr 174 30 synthesis synthesis NN cord-275664-qbafkxtr 174 31 are be VBP cord-275664-qbafkxtr 174 32 closely closely RB cord-275664-qbafkxtr 174 33 related relate VBN cord-275664-qbafkxtr 174 34 in in IN cord-275664-qbafkxtr 174 35 the the DT cord-275664-qbafkxtr 174 36 replication replication NN cord-275664-qbafkxtr 174 37 of of IN cord-275664-qbafkxtr 174 38 PCV2 PCV2 NNP cord-275664-qbafkxtr 174 39 . . . cord-275664-qbafkxtr 175 1 However however RB cord-275664-qbafkxtr 175 2 , , , cord-275664-qbafkxtr 175 3 a a DT cord-275664-qbafkxtr 175 4 detailed detailed JJ cord-275664-qbafkxtr 175 5 mechanistic mechanistic JJ cord-275664-qbafkxtr 175 6 understanding understanding NN cord-275664-qbafkxtr 175 7 of of IN cord-275664-qbafkxtr 175 8 the the DT cord-275664-qbafkxtr 175 9 inhibition inhibition NN cord-275664-qbafkxtr 175 10 of of IN cord-275664-qbafkxtr 175 11 ERK ERK NNP cord-275664-qbafkxtr 175 12 activation activation NN cord-275664-qbafkxtr 175 13 on on IN cord-275664-qbafkxtr 175 14 PCV2 pcv2 PRP$ cord-275664-qbafkxtr 175 15 replication replication NN cord-275664-qbafkxtr 175 16 requires require VBZ cord-275664-qbafkxtr 175 17 the the DT cord-275664-qbafkxtr 175 18 identification identification NN cord-275664-qbafkxtr 175 19 of of IN cord-275664-qbafkxtr 175 20 particular particular JJ cord-275664-qbafkxtr 175 21 cellular cellular JJ cord-275664-qbafkxtr 175 22 factors factor NNS cord-275664-qbafkxtr 175 23 which which WDT cord-275664-qbafkxtr 175 24 are be VBP cord-275664-qbafkxtr 175 25 the the DT cord-275664-qbafkxtr 175 26 components component NNS cord-275664-qbafkxtr 175 27 of of IN cord-275664-qbafkxtr 175 28 the the DT cord-275664-qbafkxtr 175 29 ERK ERK NNP cord-275664-qbafkxtr 175 30 signaling signal VBG cord-275664-qbafkxtr 175 31 pathway pathway NN cord-275664-qbafkxtr 175 32 . . . cord-275664-qbafkxtr 176 1 In in IN cord-275664-qbafkxtr 176 2 conclusion conclusion NN cord-275664-qbafkxtr 176 3 , , , cord-275664-qbafkxtr 176 4 we -PRON- PRP cord-275664-qbafkxtr 176 5 have have VBP cord-275664-qbafkxtr 176 6 shown show VBN cord-275664-qbafkxtr 176 7 that that IN cord-275664-qbafkxtr 176 8 PCV2 pcv2 JJ cord-275664-qbafkxtr 176 9 infection infection NN cord-275664-qbafkxtr 176 10 induces induce VBZ cord-275664-qbafkxtr 176 11 the the DT cord-275664-qbafkxtr 176 12 activation activation NN cord-275664-qbafkxtr 176 13 of of IN cord-275664-qbafkxtr 176 14 ERK ERK NNP cord-275664-qbafkxtr 176 15 and and CC cord-275664-qbafkxtr 176 16 its -PRON- PRP$ cord-275664-qbafkxtr 176 17 involvement involvement NN cord-275664-qbafkxtr 176 18 in in IN cord-275664-qbafkxtr 176 19 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 176 20 activation activation NN cord-275664-qbafkxtr 176 21 in in IN cord-275664-qbafkxtr 176 22 the the DT cord-275664-qbafkxtr 176 23 cultured cultured JJ cord-275664-qbafkxtr 176 24 cells cell NNS cord-275664-qbafkxtr 176 25 and and CC cord-275664-qbafkxtr 176 26 demonstrate demonstrate VBP cord-275664-qbafkxtr 176 27 that that IN cord-275664-qbafkxtr 176 28 the the DT cord-275664-qbafkxtr 176 29 activation activation NN cord-275664-qbafkxtr 176 30 of of IN cord-275664-qbafkxtr 176 31 ERK ERK NNP cord-275664-qbafkxtr 176 32 is be VBZ cord-275664-qbafkxtr 176 33 required require VBN cord-275664-qbafkxtr 176 34 for for IN cord-275664-qbafkxtr 176 35 efficient efficient JJ cord-275664-qbafkxtr 176 36 PCV2 pcv2 JJ cord-275664-qbafkxtr 176 37 replication replication NN cord-275664-qbafkxtr 176 38 . . . cord-275664-qbafkxtr 177 1 Inhibition inhibition NN cord-275664-qbafkxtr 177 2 of of IN cord-275664-qbafkxtr 177 3 ERK ERK NNP cord-275664-qbafkxtr 177 4 activation activation NN cord-275664-qbafkxtr 177 5 significantly significantly RB cord-275664-qbafkxtr 177 6 reduces reduce VBZ cord-275664-qbafkxtr 177 7 viral viral JJ cord-275664-qbafkxtr 177 8 protein protein NN cord-275664-qbafkxtr 177 9 expression expression NN cord-275664-qbafkxtr 177 10 and and CC cord-275664-qbafkxtr 177 11 viral viral JJ cord-275664-qbafkxtr 177 12 RNA RNA NNP cord-275664-qbafkxtr 177 13 transcription transcription NN cord-275664-qbafkxtr 177 14 . . . cord-275664-qbafkxtr 178 1 The the DT cord-275664-qbafkxtr 178 2 role role NN cord-275664-qbafkxtr 178 3 of of IN cord-275664-qbafkxtr 178 4 ERK ERK NNP cord-275664-qbafkxtr 178 5 activation activation NN cord-275664-qbafkxtr 178 6 in in IN cord-275664-qbafkxtr 178 7 PCV2 pcv2 JJ cord-275664-qbafkxtr 178 8 replication replication NN cord-275664-qbafkxtr 178 9 will will MD cord-275664-qbafkxtr 178 10 contribute contribute VB cord-275664-qbafkxtr 178 11 important important JJ cord-275664-qbafkxtr 178 12 information information NN cord-275664-qbafkxtr 178 13 about about IN cord-275664-qbafkxtr 178 14 the the DT cord-275664-qbafkxtr 178 15 molecular molecular JJ cord-275664-qbafkxtr 178 16 mechanism mechanism NN cord-275664-qbafkxtr 178 17 of of IN cord-275664-qbafkxtr 178 18 PCV2 pcv2 JJ cord-275664-qbafkxtr 178 19 infection infection NN cord-275664-qbafkxtr 178 20 . . . cord-275664-qbafkxtr 179 1 The the DT cord-275664-qbafkxtr 179 2 permanent permanent JJ cord-275664-qbafkxtr 179 3 PK15 PK15 NNP cord-275664-qbafkxtr 179 4 cell cell NN cord-275664-qbafkxtr 179 5 line line NN cord-275664-qbafkxtr 179 6 , , , cord-275664-qbafkxtr 179 7 which which WDT cord-275664-qbafkxtr 179 8 was be VBD cord-275664-qbafkxtr 179 9 free free JJ cord-275664-qbafkxtr 179 10 of of IN cord-275664-qbafkxtr 179 11 PCV PCV NNP cord-275664-qbafkxtr 179 12 , , , cord-275664-qbafkxtr 179 13 was be VBD cord-275664-qbafkxtr 179 14 maintained maintain VBN cord-275664-qbafkxtr 179 15 in in IN cord-275664-qbafkxtr 179 16 minimal minimal JJ cord-275664-qbafkxtr 179 17 essential essential JJ cord-275664-qbafkxtr 179 18 medium medium NN cord-275664-qbafkxtr 179 19 ( ( -LRB- cord-275664-qbafkxtr 179 20 MEM MEM NNP cord-275664-qbafkxtr 179 21 ) ) -RRB- cord-275664-qbafkxtr 179 22 supplemented supplement VBD cord-275664-qbafkxtr 179 23 with with IN cord-275664-qbafkxtr 179 24 5 5 CD cord-275664-qbafkxtr 179 25 % % NN cord-275664-qbafkxtr 179 26 heat heat NN cord-275664-qbafkxtr 179 27 - - HYPH cord-275664-qbafkxtr 179 28 inactivated inactivate VBN cord-275664-qbafkxtr 179 29 fetal fetal JJ cord-275664-qbafkxtr 179 30 bovine bovine NN cord-275664-qbafkxtr 179 31 serum serum NN cord-275664-qbafkxtr 179 32 ( ( -LRB- cord-275664-qbafkxtr 179 33 FBS FBS NNP cord-275664-qbafkxtr 179 34 ) ) -RRB- cord-275664-qbafkxtr 179 35 , , , cord-275664-qbafkxtr 179 36 5 5 CD cord-275664-qbafkxtr 179 37 % % NN cord-275664-qbafkxtr 179 38 L l NN cord-275664-qbafkxtr 179 39 - - HYPH cord-275664-qbafkxtr 179 40 glutamine glutamine NN cord-275664-qbafkxtr 179 41 , , , cord-275664-qbafkxtr 179 42 100 100 CD cord-275664-qbafkxtr 179 43 U U NNS cord-275664-qbafkxtr 179 44 of of IN cord-275664-qbafkxtr 179 45 penicillin penicillin NN cord-275664-qbafkxtr 179 46 G G NNP cord-275664-qbafkxtr 179 47 / / SYM cord-275664-qbafkxtr 179 48 ml ml NN cord-275664-qbafkxtr 179 49 , , , cord-275664-qbafkxtr 179 50 and and CC cord-275664-qbafkxtr 179 51 100 100 CD cord-275664-qbafkxtr 179 52 μl μl NN cord-275664-qbafkxtr 179 53 of of IN cord-275664-qbafkxtr 179 54 streptomycin streptomycin NNS cord-275664-qbafkxtr 179 55 / / SYM cord-275664-qbafkxtr 179 56 ml ml NN cord-275664-qbafkxtr 179 57 at at IN cord-275664-qbafkxtr 179 58 37 37 CD cord-275664-qbafkxtr 179 59 ° ° NN cord-275664-qbafkxtr 179 60 C c NN cord-275664-qbafkxtr 179 61 in in IN cord-275664-qbafkxtr 179 62 a a DT cord-275664-qbafkxtr 179 63 humidified humidify VBN cord-275664-qbafkxtr 179 64 5 5 CD cord-275664-qbafkxtr 179 65 % % NN cord-275664-qbafkxtr 179 66 CO co NN cord-275664-qbafkxtr 179 67 2 2 CD cord-275664-qbafkxtr 179 68 incubator incubator NN cord-275664-qbafkxtr 179 69 . . . cord-275664-qbafkxtr 180 1 The the DT cord-275664-qbafkxtr 180 2 PCV2 PCV2 NNP cord-275664-qbafkxtr 180 3 virus virus NN cord-275664-qbafkxtr 180 4 used use VBN cord-275664-qbafkxtr 180 5 in in IN cord-275664-qbafkxtr 180 6 the the DT cord-275664-qbafkxtr 180 7 study study NN cord-275664-qbafkxtr 180 8 was be VBD cord-275664-qbafkxtr 180 9 originally originally RB cord-275664-qbafkxtr 180 10 isolated isolate VBN cord-275664-qbafkxtr 180 11 from from IN cord-275664-qbafkxtr 180 12 a a DT cord-275664-qbafkxtr 180 13 kidney kidney NN cord-275664-qbafkxtr 180 14 tissue tissue NN cord-275664-qbafkxtr 180 15 sample sample NN cord-275664-qbafkxtr 180 16 of of IN cord-275664-qbafkxtr 180 17 a a DT cord-275664-qbafkxtr 180 18 pig pig NN cord-275664-qbafkxtr 180 19 with with IN cord-275664-qbafkxtr 180 20 naturally naturally RB cord-275664-qbafkxtr 180 21 occurring occur VBG cord-275664-qbafkxtr 180 22 PMWS PMWS NNP cord-275664-qbafkxtr 180 23 ( ( -LRB- cord-275664-qbafkxtr 181 1 strain strain VB cord-275664-qbafkxtr 181 2 BJW BJW NNP cord-275664-qbafkxtr 181 3 ) ) -RRB- cord-275664-qbafkxtr 182 1 ( ( -LRB- cord-275664-qbafkxtr 182 2 Liu Liu NNP cord-275664-qbafkxtr 182 3 et et NNP cord-275664-qbafkxtr 182 4 al al NNP cord-275664-qbafkxtr 182 5 . . NNP cord-275664-qbafkxtr 182 6 , , , cord-275664-qbafkxtr 182 7 2005 2005 CD cord-275664-qbafkxtr 182 8 ) ) -RRB- cord-275664-qbafkxtr 182 9 . . . cord-275664-qbafkxtr 183 1 For for IN cord-275664-qbafkxtr 183 2 PCV2 pcv2 JJ cord-275664-qbafkxtr 183 3 infection infection NN cord-275664-qbafkxtr 183 4 , , , cord-275664-qbafkxtr 183 5 PK15 PK15 NNP cord-275664-qbafkxtr 183 6 cells cell NNS cord-275664-qbafkxtr 183 7 seeded seed VBD cord-275664-qbafkxtr 183 8 the the DT cord-275664-qbafkxtr 183 9 day day NN cord-275664-qbafkxtr 183 10 before before RB cord-275664-qbafkxtr 183 11 were be VBD cord-275664-qbafkxtr 183 12 infected infect VBN cord-275664-qbafkxtr 183 13 with with IN cord-275664-qbafkxtr 183 14 PCV2 pcv2 JJ cord-275664-qbafkxtr 183 15 strain strain NN cord-275664-qbafkxtr 183 16 BJW bjw NN cord-275664-qbafkxtr 183 17 at at IN cord-275664-qbafkxtr 183 18 a a DT cord-275664-qbafkxtr 183 19 multiplicity multiplicity NN cord-275664-qbafkxtr 183 20 of of IN cord-275664-qbafkxtr 183 21 infection infection NN cord-275664-qbafkxtr 183 22 ( ( -LRB- cord-275664-qbafkxtr 183 23 MOI MOI NNP cord-275664-qbafkxtr 183 24 ) ) -RRB- cord-275664-qbafkxtr 183 25 of of IN cord-275664-qbafkxtr 183 26 1 1 CD cord-275664-qbafkxtr 183 27 TCID TCID NNP cord-275664-qbafkxtr 183 28 50 50 CD cord-275664-qbafkxtr 183 29 . . . cord-275664-qbafkxtr 184 1 Cells cell NNS cord-275664-qbafkxtr 184 2 were be VBD cord-275664-qbafkxtr 184 3 additionally additionally RB cord-275664-qbafkxtr 184 4 treated treat VBN cord-275664-qbafkxtr 184 5 with with IN cord-275664-qbafkxtr 184 6 300 300 CD cord-275664-qbafkxtr 184 7 mM mm NN cord-275664-qbafkxtr 184 8 D d NN cord-275664-qbafkxtr 184 9 - - HYPH cord-275664-qbafkxtr 184 10 glucosamine glucosamine NN cord-275664-qbafkxtr 184 11 at at IN cord-275664-qbafkxtr 184 12 24 24 CD cord-275664-qbafkxtr 184 13 h h NN cord-275664-qbafkxtr 184 14 after after IN cord-275664-qbafkxtr 184 15 infection infection NN cord-275664-qbafkxtr 184 16 as as IN cord-275664-qbafkxtr 184 17 described describe VBN cord-275664-qbafkxtr 184 18 previously previously RB cord-275664-qbafkxtr 184 19 ( ( -LRB- cord-275664-qbafkxtr 184 20 Tischer Tischer NNP cord-275664-qbafkxtr 184 21 et et NNP cord-275664-qbafkxtr 184 22 al al NNP cord-275664-qbafkxtr 184 23 . . NNP cord-275664-qbafkxtr 184 24 , , , cord-275664-qbafkxtr 184 25 1987 1987 CD cord-275664-qbafkxtr 184 26 ) ) -RRB- cord-275664-qbafkxtr 184 27 . . . cord-275664-qbafkxtr 185 1 U0126 U0126 NNP cord-275664-qbafkxtr 185 2 was be VBD cord-275664-qbafkxtr 185 3 purchased purchase VBN cord-275664-qbafkxtr 185 4 from from IN cord-275664-qbafkxtr 185 5 Calbiochem Calbiochem NNP cord-275664-qbafkxtr 185 6 ( ( -LRB- cord-275664-qbafkxtr 185 7 La La NNP cord-275664-qbafkxtr 185 8 Jolla Jolla NNP cord-275664-qbafkxtr 185 9 , , , cord-275664-qbafkxtr 185 10 Calif. California NNP cord-275664-qbafkxtr 185 11 ) ) -RRB- cord-275664-qbafkxtr 185 12 . . . cord-275664-qbafkxtr 186 1 PK15 PK15 NNP cord-275664-qbafkxtr 186 2 cells cell NNS cord-275664-qbafkxtr 186 3 were be VBD cord-275664-qbafkxtr 186 4 treated treat VBN cord-275664-qbafkxtr 186 5 with with IN cord-275664-qbafkxtr 186 6 either either CC cord-275664-qbafkxtr 186 7 DMSO DMSO NNP cord-275664-qbafkxtr 186 8 which which WDT cord-275664-qbafkxtr 186 9 is be VBZ cord-275664-qbafkxtr 186 10 the the DT cord-275664-qbafkxtr 186 11 solvent solvent NN cord-275664-qbafkxtr 186 12 for for IN cord-275664-qbafkxtr 186 13 U0126 U0126 NNP cord-275664-qbafkxtr 186 14 or or CC cord-275664-qbafkxtr 186 15 various various JJ cord-275664-qbafkxtr 186 16 concentrations concentration NNS cord-275664-qbafkxtr 186 17 ( ( -LRB- cord-275664-qbafkxtr 186 18 5 5 CD cord-275664-qbafkxtr 186 19 - - SYM cord-275664-qbafkxtr 186 20 20 20 CD cord-275664-qbafkxtr 186 21 μM μM NNP cord-275664-qbafkxtr 186 22 ) ) -RRB- cord-275664-qbafkxtr 186 23 for for IN cord-275664-qbafkxtr 186 24 1 1 CD cord-275664-qbafkxtr 186 25 h h NN cord-275664-qbafkxtr 186 26 prior prior RB cord-275664-qbafkxtr 186 27 to to IN cord-275664-qbafkxtr 186 28 infection infection NN cord-275664-qbafkxtr 186 29 . . . cord-275664-qbafkxtr 187 1 After after IN cord-275664-qbafkxtr 187 2 1 1 CD cord-275664-qbafkxtr 187 3 h h NN cord-275664-qbafkxtr 187 4 of of IN cord-275664-qbafkxtr 187 5 virus virus NN cord-275664-qbafkxtr 187 6 adsorption adsorption NN cord-275664-qbafkxtr 187 7 , , , cord-275664-qbafkxtr 187 8 the the DT cord-275664-qbafkxtr 187 9 virus virus NN cord-275664-qbafkxtr 187 10 inoculum inoculum NN cord-275664-qbafkxtr 187 11 was be VBD cord-275664-qbafkxtr 187 12 removed remove VBN cord-275664-qbafkxtr 187 13 and and CC cord-275664-qbafkxtr 187 14 fresh fresh JJ cord-275664-qbafkxtr 187 15 basal basal NN cord-275664-qbafkxtr 187 16 medium medium NN cord-275664-qbafkxtr 187 17 containing contain VBG cord-275664-qbafkxtr 187 18 fresh fresh JJ cord-275664-qbafkxtr 187 19 inhibitor inhibitor NN cord-275664-qbafkxtr 187 20 was be VBD cord-275664-qbafkxtr 187 21 added add VBN cord-275664-qbafkxtr 187 22 to to IN cord-275664-qbafkxtr 187 23 the the DT cord-275664-qbafkxtr 187 24 culture culture NN cord-275664-qbafkxtr 187 25 . . . cord-275664-qbafkxtr 188 1 The the DT cord-275664-qbafkxtr 188 2 cytotoxicity cytotoxicity NN cord-275664-qbafkxtr 188 3 of of IN cord-275664-qbafkxtr 188 4 the the DT cord-275664-qbafkxtr 188 5 inhibitors inhibitor NNS cord-275664-qbafkxtr 188 6 on on IN cord-275664-qbafkxtr 188 7 PK15 PK15 NNP cord-275664-qbafkxtr 188 8 cells cell NNS cord-275664-qbafkxtr 188 9 was be VBD cord-275664-qbafkxtr 188 10 determined determine VBN cord-275664-qbafkxtr 188 11 by by IN cord-275664-qbafkxtr 188 12 trypan trypan NNP cord-275664-qbafkxtr 188 13 blue blue JJ cord-275664-qbafkxtr 188 14 exclusion exclusion NN cord-275664-qbafkxtr 188 15 dye dye NN cord-275664-qbafkxtr 188 16 staining staining NN cord-275664-qbafkxtr 188 17 . . . cord-275664-qbafkxtr 189 1 It -PRON- PRP cord-275664-qbafkxtr 189 2 was be VBD cord-275664-qbafkxtr 189 3 noted note VBN cord-275664-qbafkxtr 189 4 that that IN cord-275664-qbafkxtr 189 5 throughout throughout IN cord-275664-qbafkxtr 189 6 all all DT cord-275664-qbafkxtr 189 7 doses dose NNS cord-275664-qbafkxtr 189 8 of of IN cord-275664-qbafkxtr 189 9 the the DT cord-275664-qbafkxtr 189 10 inhibitor inhibitor NN cord-275664-qbafkxtr 189 11 used use VBN cord-275664-qbafkxtr 189 12 in in IN cord-275664-qbafkxtr 189 13 this this DT cord-275664-qbafkxtr 189 14 study study NN cord-275664-qbafkxtr 189 15 , , , cord-275664-qbafkxtr 189 16 cell cell NN cord-275664-qbafkxtr 189 17 viability viability NN cord-275664-qbafkxtr 189 18 assay assay NN cord-275664-qbafkxtr 189 19 showed show VBD cord-275664-qbafkxtr 189 20 no no DT cord-275664-qbafkxtr 189 21 detectable detectable JJ cord-275664-qbafkxtr 189 22 cell cell NN cord-275664-qbafkxtr 189 23 death death NN cord-275664-qbafkxtr 189 24 in in IN cord-275664-qbafkxtr 189 25 PK15 PK15 NNP cord-275664-qbafkxtr 189 26 cells cell NNS cord-275664-qbafkxtr 189 27 . . . cord-275664-qbafkxtr 190 1 Rabbit Rabbit NNP cord-275664-qbafkxtr 190 2 antibodies antibody NNS cord-275664-qbafkxtr 190 3 against against IN cord-275664-qbafkxtr 190 4 ERK2 ERK2 NNP cord-275664-qbafkxtr 190 5 , , , cord-275664-qbafkxtr 190 6 β β NN cord-275664-qbafkxtr 190 7 - - HYPH cord-275664-qbafkxtr 190 8 actin actin NN cord-275664-qbafkxtr 190 9 , , , cord-275664-qbafkxtr 190 10 as as RB cord-275664-qbafkxtr 190 11 well well RB cord-275664-qbafkxtr 190 12 as as IN cord-275664-qbafkxtr 190 13 phosphorylated phosphorylate VBN cord-275664-qbafkxtr 191 1 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 192 1 ( ( -LRB- cord-275664-qbafkxtr 192 2 p p NN cord-275664-qbafkxtr 192 3 - - HYPH cord-275664-qbafkxtr 192 4 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 192 5 ) ) -RRB- cord-275664-qbafkxtr 192 6 were be VBD cord-275664-qbafkxtr 192 7 purchased purchase VBN cord-275664-qbafkxtr 192 8 from from IN cord-275664-qbafkxtr 192 9 Santa Santa NNP cord-275664-qbafkxtr 192 10 Cruz Cruz NNP cord-275664-qbafkxtr 192 11 Biotechnology Biotechnology NNP cord-275664-qbafkxtr 192 12 ( ( -LRB- cord-275664-qbafkxtr 192 13 Hercules Hercules NNP cord-275664-qbafkxtr 192 14 , , , cord-275664-qbafkxtr 192 15 CA CA NNP cord-275664-qbafkxtr 192 16 ) ) -RRB- cord-275664-qbafkxtr 192 17 . . . cord-275664-qbafkxtr 193 1 Antibody antibody NN cord-275664-qbafkxtr 193 2 specific specific JJ cord-275664-qbafkxtr 193 3 for for IN cord-275664-qbafkxtr 193 4 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 193 5 forms form NNS cord-275664-qbafkxtr 193 6 of of IN cord-275664-qbafkxtr 193 7 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 193 8 ( ( -LRB- cord-275664-qbafkxtr 193 9 p p NNP cord-275664-qbafkxtr 193 10 - - : cord-275664-qbafkxtr 193 11 Elk-1 Elk-1 NNP cord-275664-qbafkxtr 193 12 ) ) -RRB- cord-275664-qbafkxtr 193 13 was be VBD cord-275664-qbafkxtr 193 14 obtained obtain VBN cord-275664-qbafkxtr 193 15 from from IN cord-275664-qbafkxtr 193 16 Cell Cell NNP cord-275664-qbafkxtr 193 17 Signaling Signaling NNP cord-275664-qbafkxtr 193 18 Technology Technology NNP cord-275664-qbafkxtr 193 19 . . . cord-275664-qbafkxtr 194 1 Horseradish horseradish NN cord-275664-qbafkxtr 194 2 peroxidise peroxidise NN cord-275664-qbafkxtr 194 3 ( ( -LRB- cord-275664-qbafkxtr 194 4 HRP)-linked hrp)-linked JJ cord-275664-qbafkxtr 194 5 secondary secondary JJ cord-275664-qbafkxtr 194 6 antibodies antibody NNS cord-275664-qbafkxtr 194 7 were be VBD cord-275664-qbafkxtr 194 8 purchased purchase VBN cord-275664-qbafkxtr 194 9 from from IN cord-275664-qbafkxtr 194 10 Sigma Sigma NNP cord-275664-qbafkxtr 194 11 . . . cord-275664-qbafkxtr 195 1 FACE face NN cord-275664-qbafkxtr 195 2 kit kit NN cord-275664-qbafkxtr 195 3 to to TO cord-275664-qbafkxtr 195 4 monitor monitor VB cord-275664-qbafkxtr 195 5 the the DT cord-275664-qbafkxtr 195 6 levels level NNS cord-275664-qbafkxtr 195 7 of of IN cord-275664-qbafkxtr 195 8 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 195 9 MAPK MAPK NNP cord-275664-qbafkxtr 195 10 activation activation NN cord-275664-qbafkxtr 195 11 was be VBD cord-275664-qbafkxtr 195 12 obtained obtain VBN cord-275664-qbafkxtr 195 13 from from IN cord-275664-qbafkxtr 195 14 Active active JJ cord-275664-qbafkxtr 195 15 Motif motif NN cord-275664-qbafkxtr 195 16 . . . cord-275664-qbafkxtr 196 1 Procedure procedure NN cord-275664-qbafkxtr 196 2 was be VBD cord-275664-qbafkxtr 196 3 performed perform VBN cord-275664-qbafkxtr 196 4 strictly strictly RB cord-275664-qbafkxtr 196 5 according accord VBG cord-275664-qbafkxtr 196 6 to to IN cord-275664-qbafkxtr 196 7 the the DT cord-275664-qbafkxtr 196 8 manufacturer manufacturer NN cord-275664-qbafkxtr 196 9 's 's POS cord-275664-qbafkxtr 196 10 instructions instruction NNS cord-275664-qbafkxtr 196 11 . . . cord-275664-qbafkxtr 197 1 Briefly briefly RB cord-275664-qbafkxtr 197 2 , , , cord-275664-qbafkxtr 197 3 PK15 PK15 NNP cord-275664-qbafkxtr 197 4 cells cell NNS cord-275664-qbafkxtr 197 5 were be VBD cord-275664-qbafkxtr 197 6 seeded seed VBN cord-275664-qbafkxtr 197 7 in in IN cord-275664-qbafkxtr 197 8 96-well 96-well CD cord-275664-qbafkxtr 197 9 plates plate NNS cord-275664-qbafkxtr 197 10 1 1 CD cord-275664-qbafkxtr 197 11 day day NN cord-275664-qbafkxtr 197 12 prior prior RB cord-275664-qbafkxtr 197 13 to to IN cord-275664-qbafkxtr 197 14 infection infection NN cord-275664-qbafkxtr 197 15 . . . cord-275664-qbafkxtr 198 1 After after IN cord-275664-qbafkxtr 198 2 treatment treatment NN cord-275664-qbafkxtr 198 3 and/or and/or CC cord-275664-qbafkxtr 198 4 infection infection NN cord-275664-qbafkxtr 198 5 , , , cord-275664-qbafkxtr 198 6 cells cell NNS cord-275664-qbafkxtr 198 7 were be VBD cord-275664-qbafkxtr 198 8 fixed fix VBN cord-275664-qbafkxtr 198 9 with with IN cord-275664-qbafkxtr 198 10 4 4 CD cord-275664-qbafkxtr 198 11 % % NN cord-275664-qbafkxtr 198 12 formaldehyde formaldehyde NN cord-275664-qbafkxtr 198 13 in in IN cord-275664-qbafkxtr 198 14 PBS PBS NNP cord-275664-qbafkxtr 198 15 . . . cord-275664-qbafkxtr 199 1 After after IN cord-275664-qbafkxtr 199 2 washing washing NN cord-275664-qbafkxtr 199 3 and and CC cord-275664-qbafkxtr 199 4 blocking blocking NN cord-275664-qbafkxtr 199 5 , , , cord-275664-qbafkxtr 199 6 cells cell NNS cord-275664-qbafkxtr 199 7 were be VBD cord-275664-qbafkxtr 199 8 reacted react VBN cord-275664-qbafkxtr 199 9 overnight overnight RB cord-275664-qbafkxtr 199 10 with with IN cord-275664-qbafkxtr 199 11 an an DT cord-275664-qbafkxtr 199 12 anti anti JJ cord-275664-qbafkxtr 199 13 - - JJ cord-275664-qbafkxtr 199 14 ERK1/2 erk1/2 JJ cord-275664-qbafkxtr 199 15 or or CC cord-275664-qbafkxtr 199 16 anti anti JJ cord-275664-qbafkxtr 199 17 - - JJ cord-275664-qbafkxtr 199 18 phospho phospho JJ cord-275664-qbafkxtr 199 19 - - HYPH cord-275664-qbafkxtr 199 20 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 199 21 antibody antibody NN cord-275664-qbafkxtr 199 22 . . . cord-275664-qbafkxtr 200 1 Following follow VBG cord-275664-qbafkxtr 200 2 incubation incubation NN cord-275664-qbafkxtr 200 3 with with IN cord-275664-qbafkxtr 200 4 a a DT cord-275664-qbafkxtr 200 5 HRP HRP NNP cord-275664-qbafkxtr 200 6 - - HYPH cord-275664-qbafkxtr 200 7 conjugated conjugate VBN cord-275664-qbafkxtr 200 8 secondary secondary JJ cord-275664-qbafkxtr 200 9 antibody antibody NN cord-275664-qbafkxtr 200 10 , , , cord-275664-qbafkxtr 200 11 colorimetric colorimetric JJ cord-275664-qbafkxtr 200 12 analysis analysis NN cord-275664-qbafkxtr 200 13 was be VBD cord-275664-qbafkxtr 200 14 performed perform VBN cord-275664-qbafkxtr 200 15 . . . cord-275664-qbafkxtr 201 1 A450 A450 NNP cord-275664-qbafkxtr 201 2 was be VBD cord-275664-qbafkxtr 201 3 determined determine VBN cord-275664-qbafkxtr 201 4 using use VBG cord-275664-qbafkxtr 201 5 a a DT cord-275664-qbafkxtr 201 6 plate plate NN cord-275664-qbafkxtr 201 7 spectrophotometer spectrophotometer NN cord-275664-qbafkxtr 201 8 . . . cord-275664-qbafkxtr 202 1 Total total JJ cord-275664-qbafkxtr 202 2 cell cell NN cord-275664-qbafkxtr 202 3 RNAs rna NNS cord-275664-qbafkxtr 202 4 were be VBD cord-275664-qbafkxtr 202 5 prepared prepare VBN cord-275664-qbafkxtr 202 6 from from IN cord-275664-qbafkxtr 202 7 PCV2-infected PCV2-infected NNP cord-275664-qbafkxtr 203 1 PK15 PK15 NNP cord-275664-qbafkxtr 203 2 cell cell NN cord-275664-qbafkxtr 203 3 48 48 CD cord-275664-qbafkxtr 203 4 h h NN cord-275664-qbafkxtr 203 5 after after IN cord-275664-qbafkxtr 203 6 being be VBG cord-275664-qbafkxtr 203 7 treated treat VBN cord-275664-qbafkxtr 203 8 with with IN cord-275664-qbafkxtr 203 9 various various JJ cord-275664-qbafkxtr 203 10 concentrations concentration NNS cord-275664-qbafkxtr 203 11 of of IN cord-275664-qbafkxtr 203 12 the the DT cord-275664-qbafkxtr 203 13 inhibitor inhibitor NN cord-275664-qbafkxtr 203 14 U0126 U0126 NNP cord-275664-qbafkxtr 203 15 by by IN cord-275664-qbafkxtr 203 16 using use VBG cord-275664-qbafkxtr 203 17 Trizol Trizol NNP cord-275664-qbafkxtr 203 18 RNA RNA NNP cord-275664-qbafkxtr 203 19 extract extract NN cord-275664-qbafkxtr 203 20 reagent reagent NN cord-275664-qbafkxtr 203 21 ( ( -LRB- cord-275664-qbafkxtr 203 22 Invitrogen Invitrogen NNP cord-275664-qbafkxtr 203 23 ) ) -RRB- cord-275664-qbafkxtr 203 24 . . . cord-275664-qbafkxtr 204 1 The the DT cord-275664-qbafkxtr 204 2 following follow VBG cord-275664-qbafkxtr 204 3 primers primer NNS cord-275664-qbafkxtr 204 4 were be VBD cord-275664-qbafkxtr 204 5 used use VBN cord-275664-qbafkxtr 204 6 : : : cord-275664-qbafkxtr 204 7 ORF2 ORF2 NNP cord-275664-qbafkxtr 204 8 ( ( -LRB- cord-275664-qbafkxtr 204 9 sense sense NN cord-275664-qbafkxtr 204 10 ) ) -RRB- cord-275664-qbafkxtr 204 11 ( ( -LRB- cord-275664-qbafkxtr 204 12 5′-ATCAAGCGAACCACAG-3′ 5′-atcaagcgaaccacag-3′ CD cord-275664-qbafkxtr 204 13 ) ) -RRB- cord-275664-qbafkxtr 204 14 and and CC cord-275664-qbafkxtr 204 15 ORF2 ORF2 NNP cord-275664-qbafkxtr 204 16 ( ( -LRB- cord-275664-qbafkxtr 204 17 antisense antisense NN cord-275664-qbafkxtr 204 18 ) ) -RRB- cord-275664-qbafkxtr 205 1 ( ( -LRB- cord-275664-qbafkxtr 205 2 5′-GGTCATAGGTGAGGGGC-3′ 5′-ggtcataggtgaggggc-3′ CD cord-275664-qbafkxtr 205 3 ) ) -RRB- cord-275664-qbafkxtr 205 4 for for IN cord-275664-qbafkxtr 205 5 PCV2 PCV2 NNP cord-275664-qbafkxtr 205 6 ORF2 ORF2 NNP cord-275664-qbafkxtr 205 7 and and CC cord-275664-qbafkxtr 205 8 sense sense NN cord-275664-qbafkxtr 205 9 ( ( -LRB- cord-275664-qbafkxtr 205 10 5′-CACGCCATCCTGCGTCTGGA-3′ 5′-cacgccatcctgcgtctgga-3′ CD cord-275664-qbafkxtr 205 11 ) ) -RRB- cord-275664-qbafkxtr 205 12 and and CC cord-275664-qbafkxtr 205 13 antisense antisense NN cord-275664-qbafkxtr 205 14 ( ( -LRB- cord-275664-qbafkxtr 205 15 5′-AGCACC 5′-agcacc CD cord-275664-qbafkxtr 205 16 - - HYPH cord-275664-qbafkxtr 205 17 GTGTTGGCGTAGAG GTGTTGGCGTAGAG NNP cord-275664-qbafkxtr 205 18 ) ) -RRB- cord-275664-qbafkxtr 205 19 for for IN cord-275664-qbafkxtr 205 20 β β NN cord-275664-qbafkxtr 205 21 - - HYPH cord-275664-qbafkxtr 205 22 actin actin NN cord-275664-qbafkxtr 205 23 . . . cord-275664-qbafkxtr 206 1 The the DT cord-275664-qbafkxtr 206 2 RNA RNA NNP cord-275664-qbafkxtr 206 3 samples sample NNS cord-275664-qbafkxtr 206 4 were be VBD cord-275664-qbafkxtr 206 5 incubated incubate VBN cord-275664-qbafkxtr 206 6 with with IN cord-275664-qbafkxtr 206 7 DNase DNase NNP cord-275664-qbafkxtr 206 8 I i NN cord-275664-qbafkxtr 206 9 for for IN cord-275664-qbafkxtr 206 10 60 60 CD cord-275664-qbafkxtr 206 11 min min NN cord-275664-qbafkxtr 206 12 at at IN cord-275664-qbafkxtr 206 13 37 37 CD cord-275664-qbafkxtr 206 14 ° ° NN cord-275664-qbafkxtr 206 15 C c NN cord-275664-qbafkxtr 206 16 to to TO cord-275664-qbafkxtr 206 17 remove remove VB cord-275664-qbafkxtr 206 18 any any DT cord-275664-qbafkxtr 206 19 contaminating contaminate VBG cord-275664-qbafkxtr 206 20 viral viral JJ cord-275664-qbafkxtr 206 21 DNA dna NN cord-275664-qbafkxtr 206 22 . . . cord-275664-qbafkxtr 207 1 cDNAs cDNAs NNPS cord-275664-qbafkxtr 207 2 were be VBD cord-275664-qbafkxtr 207 3 reverse reverse JJ cord-275664-qbafkxtr 207 4 transcribed transcribe VBN cord-275664-qbafkxtr 207 5 from from IN cord-275664-qbafkxtr 207 6 total total JJ cord-275664-qbafkxtr 207 7 RNAs rna NNS cord-275664-qbafkxtr 207 8 by by IN cord-275664-qbafkxtr 207 9 the the DT cord-275664-qbafkxtr 207 10 use use NN cord-275664-qbafkxtr 207 11 of of IN cord-275664-qbafkxtr 207 12 antisense antisense NN cord-275664-qbafkxtr 207 13 primers primer NNS cord-275664-qbafkxtr 207 14 and and CC cord-275664-qbafkxtr 207 15 the the DT cord-275664-qbafkxtr 207 16 First First NNP cord-275664-qbafkxtr 207 17 - - HYPH cord-275664-qbafkxtr 207 18 Strand strand NN cord-275664-qbafkxtr 207 19 synthesis synthesis NN cord-275664-qbafkxtr 207 20 system system NN cord-275664-qbafkxtr 207 21 ( ( -LRB- cord-275664-qbafkxtr 207 22 Avian Avian NNP cord-275664-qbafkxtr 207 23 Myeloblastosis Myeloblastosis NNP cord-275664-qbafkxtr 207 24 Virus Virus NNP cord-275664-qbafkxtr 207 25 Reverse Reverse NNP cord-275664-qbafkxtr 207 26 Transcriptase Transcriptase NNP cord-275664-qbafkxtr 207 27 kit kit NN cord-275664-qbafkxtr 207 28 ; ; : cord-275664-qbafkxtr 207 29 Roche Roche NNP cord-275664-qbafkxtr 207 30 ) ) -RRB- cord-275664-qbafkxtr 207 31 . . . cord-275664-qbafkxtr 208 1 Quantitative quantitative JJ cord-275664-qbafkxtr 208 2 real real JJ cord-275664-qbafkxtr 208 3 - - HYPH cord-275664-qbafkxtr 208 4 time time NN cord-275664-qbafkxtr 208 5 PCR PCR NNP cord-275664-qbafkxtr 208 6 was be VBD cord-275664-qbafkxtr 208 7 performed perform VBN cord-275664-qbafkxtr 208 8 on on IN cord-275664-qbafkxtr 208 9 a a DT cord-275664-qbafkxtr 208 10 LightCycler LightCycler NNP cord-275664-qbafkxtr 208 11 ( ( -LRB- cord-275664-qbafkxtr 208 12 Roche Roche NNP cord-275664-qbafkxtr 208 13 ) ) -RRB- cord-275664-qbafkxtr 208 14 instrument instrument NN cord-275664-qbafkxtr 208 15 according accord VBG cord-275664-qbafkxtr 208 16 to to IN cord-275664-qbafkxtr 208 17 the the DT cord-275664-qbafkxtr 208 18 instructions instruction NNS cord-275664-qbafkxtr 208 19 of of IN cord-275664-qbafkxtr 208 20 the the DT cord-275664-qbafkxtr 208 21 LightCycler LightCycler NNP cord-275664-qbafkxtr 208 22 Fast Fast NNP cord-275664-qbafkxtr 208 23 Start Start NNP cord-275664-qbafkxtr 208 24 DNA DNA NNP cord-275664-qbafkxtr 208 25 Master Master NNP cord-275664-qbafkxtr 208 26 plus plus CC cord-275664-qbafkxtr 208 27 SYBR SYBR NNP cord-275664-qbafkxtr 208 28 Green Green NNP cord-275664-qbafkxtr 209 1 I -PRON- PRP cord-275664-qbafkxtr 209 2 kit kit VBP cord-275664-qbafkxtr 209 3 ( ( -LRB- cord-275664-qbafkxtr 209 4 Roche Roche NNP cord-275664-qbafkxtr 209 5 ) ) -RRB- cord-275664-qbafkxtr 209 6 . . . cord-275664-qbafkxtr 210 1 The the DT cord-275664-qbafkxtr 210 2 PCR PCR NNP cord-275664-qbafkxtr 210 3 parameters parameter NNS cord-275664-qbafkxtr 210 4 consisted consist VBD cord-275664-qbafkxtr 210 5 of of IN cord-275664-qbafkxtr 210 6 an an DT cord-275664-qbafkxtr 210 7 initial initial JJ cord-275664-qbafkxtr 210 8 denaturation denaturation NN cord-275664-qbafkxtr 210 9 at at IN cord-275664-qbafkxtr 210 10 94 94 CD cord-275664-qbafkxtr 210 11 ° ° NN cord-275664-qbafkxtr 210 12 C c NN cord-275664-qbafkxtr 210 13 for for IN cord-275664-qbafkxtr 210 14 5 5 CD cord-275664-qbafkxtr 210 15 min min NN cord-275664-qbafkxtr 210 16 , , , cord-275664-qbafkxtr 210 17 followed follow VBN cord-275664-qbafkxtr 210 18 by by IN cord-275664-qbafkxtr 210 19 40 40 CD cord-275664-qbafkxtr 210 20 cycles cycle NNS cord-275664-qbafkxtr 210 21 of of IN cord-275664-qbafkxtr 210 22 94 94 CD cord-275664-qbafkxtr 210 23 ° ° NN cord-275664-qbafkxtr 210 24 C c NN cord-275664-qbafkxtr 210 25 for for IN cord-275664-qbafkxtr 210 26 10 10 CD cord-275664-qbafkxtr 210 27 s s NN cord-275664-qbafkxtr 210 28 , , , cord-275664-qbafkxtr 210 29 52 52 CD cord-275664-qbafkxtr 210 30 ° ° NN cord-275664-qbafkxtr 210 31 C c NN cord-275664-qbafkxtr 210 32 for for IN cord-275664-qbafkxtr 210 33 5 5 CD cord-275664-qbafkxtr 210 34 s s NNPS cord-275664-qbafkxtr 210 35 and and CC cord-275664-qbafkxtr 210 36 72 72 CD cord-275664-qbafkxtr 210 37 ° ° NN cord-275664-qbafkxtr 210 38 C c NN cord-275664-qbafkxtr 210 39 for for IN cord-275664-qbafkxtr 210 40 10 10 CD cord-275664-qbafkxtr 211 1 s. s. NNP cord-275664-qbafkxtr 211 2 Subsequent subsequent JJ cord-275664-qbafkxtr 211 3 melting melt VBG cord-275664-qbafkxtr 211 4 curve curve NN cord-275664-qbafkxtr 211 5 analysis analysis NN cord-275664-qbafkxtr 211 6 and and CC cord-275664-qbafkxtr 211 7 C C NNP cord-275664-qbafkxtr 211 8 T T NNP cord-275664-qbafkxtr 211 9 value value NN cord-275664-qbafkxtr 211 10 determination determination NN cord-275664-qbafkxtr 211 11 were be VBD cord-275664-qbafkxtr 211 12 performed perform VBN cord-275664-qbafkxtr 211 13 using use VBG cord-275664-qbafkxtr 211 14 Roche Roche NNP cord-275664-qbafkxtr 211 15 LightCycler LightCycler NNP cord-275664-qbafkxtr 211 16 software software NN cord-275664-qbafkxtr 211 17 version version NN cord-275664-qbafkxtr 211 18 3.5 3.5 CD cord-275664-qbafkxtr 211 19 . . . cord-275664-qbafkxtr 212 1 Each each DT cord-275664-qbafkxtr 212 2 sample sample NN cord-275664-qbafkxtr 212 3 was be VBD cord-275664-qbafkxtr 212 4 run run VBN cord-275664-qbafkxtr 212 5 in in IN cord-275664-qbafkxtr 212 6 triplicate triplicate NN cord-275664-qbafkxtr 212 7 . . . cord-275664-qbafkxtr 213 1 The the DT cord-275664-qbafkxtr 213 2 relative relative JJ cord-275664-qbafkxtr 213 3 amount amount NN cord-275664-qbafkxtr 213 4 of of IN cord-275664-qbafkxtr 213 5 target target NN cord-275664-qbafkxtr 214 1 viral viral JJ cord-275664-qbafkxtr 214 2 mRNA mRNA NNP cord-275664-qbafkxtr 214 3 was be VBD cord-275664-qbafkxtr 214 4 normalized normalize VBN cord-275664-qbafkxtr 214 5 to to IN cord-275664-qbafkxtr 214 6 that that DT cord-275664-qbafkxtr 214 7 of of IN cord-275664-qbafkxtr 214 8 β β NN cord-275664-qbafkxtr 214 9 - - HYPH cord-275664-qbafkxtr 214 10 actin actin JJ cord-275664-qbafkxtr 214 11 mRNA mrna NN cord-275664-qbafkxtr 214 12 in in IN cord-275664-qbafkxtr 214 13 the the DT cord-275664-qbafkxtr 214 14 same same JJ cord-275664-qbafkxtr 214 15 sample sample NN cord-275664-qbafkxtr 214 16 . . . cord-275664-qbafkxtr 215 1 PK15 PK15 NNP cord-275664-qbafkxtr 215 2 monolayer monolayer NN cord-275664-qbafkxtr 215 3 cells cell NNS cord-275664-qbafkxtr 215 4 seeded seed VBN cord-275664-qbafkxtr 215 5 in in IN cord-275664-qbafkxtr 215 6 24-well 24-well CD cord-275664-qbafkxtr 215 7 culture culture NN cord-275664-qbafkxtr 215 8 plates plate NNS cord-275664-qbafkxtr 215 9 were be VBD cord-275664-qbafkxtr 215 10 infected infect VBN cord-275664-qbafkxtr 215 11 with with IN cord-275664-qbafkxtr 215 12 PCV2 pcv2 JJ cord-275664-qbafkxtr 215 13 strain strain NN cord-275664-qbafkxtr 215 14 BJW bjw NN cord-275664-qbafkxtr 215 15 . . . cord-275664-qbafkxtr 216 1 At at IN cord-275664-qbafkxtr 216 2 72 72 CD cord-275664-qbafkxtr 216 3 h h NN cord-275664-qbafkxtr 216 4 , , , cord-275664-qbafkxtr 216 5 the the DT cord-275664-qbafkxtr 216 6 cells cell NNS cord-275664-qbafkxtr 216 7 were be VBD cord-275664-qbafkxtr 216 8 washed wash VBN cord-275664-qbafkxtr 216 9 with with IN cord-275664-qbafkxtr 216 10 phosphate phosphate NN cord-275664-qbafkxtr 216 11 - - HYPH cord-275664-qbafkxtr 216 12 buffered buffer VBN cord-275664-qbafkxtr 216 13 saline saline NN cord-275664-qbafkxtr 216 14 ( ( -LRB- cord-275664-qbafkxtr 216 15 PBS PBS NNP cord-275664-qbafkxtr 216 16 ) ) -RRB- cord-275664-qbafkxtr 216 17 and and CC cord-275664-qbafkxtr 216 18 fixed fix VBN cord-275664-qbafkxtr 216 19 in in IN cord-275664-qbafkxtr 216 20 4 4 CD cord-275664-qbafkxtr 216 21 % % NN cord-275664-qbafkxtr 216 22 paraformaldehyde paraformaldehyde NNP cord-275664-qbafkxtr 216 23 ( ( -LRB- cord-275664-qbafkxtr 216 24 PFA PFA NNP cord-275664-qbafkxtr 216 25 ) ) -RRB- cord-275664-qbafkxtr 216 26 . . . cord-275664-qbafkxtr 217 1 After after IN cord-275664-qbafkxtr 217 2 three three CD cord-275664-qbafkxtr 217 3 washes wash NNS cord-275664-qbafkxtr 217 4 , , , cord-275664-qbafkxtr 217 5 the the DT cord-275664-qbafkxtr 217 6 cells cell NNS cord-275664-qbafkxtr 217 7 were be VBD cord-275664-qbafkxtr 217 8 incubated incubate VBN cord-275664-qbafkxtr 217 9 with with IN cord-275664-qbafkxtr 217 10 mouse mouse NN cord-275664-qbafkxtr 217 11 anti anti JJ cord-275664-qbafkxtr 217 12 - - JJ cord-275664-qbafkxtr 217 13 ORF2 orf2 JJ cord-275664-qbafkxtr 217 14 antibody antibody NN cord-275664-qbafkxtr 217 15 diluted dilute VBN cord-275664-qbafkxtr 217 16 in in IN cord-275664-qbafkxtr 217 17 3 3 CD cord-275664-qbafkxtr 217 18 % % NN cord-275664-qbafkxtr 217 19 bovine bovine JJ cord-275664-qbafkxtr 217 20 serum serum NN cord-275664-qbafkxtr 217 21 albumin albumin NN cord-275664-qbafkxtr 217 22 ( ( -LRB- cord-275664-qbafkxtr 217 23 BSA)-PBS bsa)-pb NNS cord-275664-qbafkxtr 217 24 at at IN cord-275664-qbafkxtr 217 25 room room NN cord-275664-qbafkxtr 217 26 temperature temperature NN cord-275664-qbafkxtr 217 27 ( ( -LRB- cord-275664-qbafkxtr 217 28 RT RT NNP cord-275664-qbafkxtr 217 29 ) ) -RRB- cord-275664-qbafkxtr 217 30 for for IN cord-275664-qbafkxtr 217 31 1 1 CD cord-275664-qbafkxtr 217 32 h. h. NN cord-275664-qbafkxtr 218 1 After after IN cord-275664-qbafkxtr 218 2 three three CD cord-275664-qbafkxtr 218 3 further further JJ cord-275664-qbafkxtr 218 4 washes wash NNS cord-275664-qbafkxtr 218 5 , , , cord-275664-qbafkxtr 218 6 cells cell NNS cord-275664-qbafkxtr 218 7 were be VBD cord-275664-qbafkxtr 218 8 incubated incubate VBN cord-275664-qbafkxtr 218 9 with with IN cord-275664-qbafkxtr 218 10 fluorescein fluorescein NN cord-275664-qbafkxtr 218 11 isothiocyanate isothiocyanate NN cord-275664-qbafkxtr 218 12 ( ( -LRB- cord-275664-qbafkxtr 218 13 FITC)-conjugated fitc)-conjugate VBN cord-275664-qbafkxtr 218 14 antimouse antimouse IN cord-275664-qbafkxtr 218 15 immunoglobulin immunoglobulin NN cord-275664-qbafkxtr 218 16 G G NNP cord-275664-qbafkxtr 218 17 ( ( -LRB- cord-275664-qbafkxtr 218 18 Sigma Sigma NNP cord-275664-qbafkxtr 218 19 ) ) -RRB- cord-275664-qbafkxtr 218 20 at at IN cord-275664-qbafkxtr 218 21 RT RT NNP cord-275664-qbafkxtr 218 22 for for IN cord-275664-qbafkxtr 218 23 1 1 CD cord-275664-qbafkxtr 218 24 h h NN cord-275664-qbafkxtr 218 25 and and CC cord-275664-qbafkxtr 218 26 washed wash VBD cord-275664-qbafkxtr 218 27 with with IN cord-275664-qbafkxtr 218 28 PBS PBS NNP cord-275664-qbafkxtr 218 29 three three CD cord-275664-qbafkxtr 218 30 times time NNS cord-275664-qbafkxtr 218 31 . . . cord-275664-qbafkxtr 219 1 The the DT cord-275664-qbafkxtr 219 2 cells cell NNS cord-275664-qbafkxtr 219 3 were be VBD cord-275664-qbafkxtr 219 4 examined examine VBN cord-275664-qbafkxtr 219 5 under under IN cord-275664-qbafkxtr 219 6 a a DT cord-275664-qbafkxtr 219 7 fluorescence fluorescence NN cord-275664-qbafkxtr 219 8 microscopy microscopy NN cord-275664-qbafkxtr 219 9 and and CC cord-275664-qbafkxtr 219 10 cells cell NNS cord-275664-qbafkxtr 219 11 positive positive JJ cord-275664-qbafkxtr 219 12 for for IN cord-275664-qbafkxtr 219 13 PCV2 pcv2 JJ cord-275664-qbafkxtr 219 14 viral viral JJ cord-275664-qbafkxtr 219 15 antigens antigen NNS cord-275664-qbafkxtr 219 16 were be VBD cord-275664-qbafkxtr 219 17 counted count VBN cord-275664-qbafkxtr 219 18 in in IN cord-275664-qbafkxtr 219 19 six six CD cord-275664-qbafkxtr 219 20 fields field NNS cord-275664-qbafkxtr 219 21 of of IN cord-275664-qbafkxtr 219 22 view view NN cord-275664-qbafkxtr 219 23 . . . cord-275664-qbafkxtr 220 1 Whole whole JJ cord-275664-qbafkxtr 220 2 cell cell NN cord-275664-qbafkxtr 220 3 lysate lysate NN cord-275664-qbafkxtr 220 4 extracts extract NNS cord-275664-qbafkxtr 220 5 from from IN cord-275664-qbafkxtr 220 6 PK15 PK15 NNP cord-275664-qbafkxtr 220 7 cells cell NNS cord-275664-qbafkxtr 220 8 after after IN cord-275664-qbafkxtr 220 9 infection infection NN cord-275664-qbafkxtr 220 10 at at IN cord-275664-qbafkxtr 220 11 various various JJ cord-275664-qbafkxtr 220 12 time time NN cord-275664-qbafkxtr 220 13 points point NNS cord-275664-qbafkxtr 220 14 were be VBD cord-275664-qbafkxtr 220 15 prepared prepare VBN cord-275664-qbafkxtr 220 16 with with IN cord-275664-qbafkxtr 220 17 the the DT cord-275664-qbafkxtr 220 18 Nuclear Nuclear NNP cord-275664-qbafkxtr 220 19 Extract Extract NNP cord-275664-qbafkxtr 220 20 kit kit NN cord-275664-qbafkxtr 220 21 ( ( -LRB- cord-275664-qbafkxtr 220 22 Active active JJ cord-275664-qbafkxtr 220 23 Motif motif NN cord-275664-qbafkxtr 220 24 ) ) -RRB- cord-275664-qbafkxtr 220 25 according accord VBG cord-275664-qbafkxtr 220 26 to to IN cord-275664-qbafkxtr 220 27 the the DT cord-275664-qbafkxtr 220 28 manufacturer manufacturer NN cord-275664-qbafkxtr 220 29 's 's POS cord-275664-qbafkxtr 220 30 protocol protocol NN cord-275664-qbafkxtr 220 31 . . . cord-275664-qbafkxtr 221 1 Protein protein NN cord-275664-qbafkxtr 221 2 concentration concentration NN cord-275664-qbafkxtr 221 3 was be VBD cord-275664-qbafkxtr 221 4 determined determine VBN cord-275664-qbafkxtr 221 5 by by IN cord-275664-qbafkxtr 221 6 the the DT cord-275664-qbafkxtr 221 7 Bio Bio NNP cord-275664-qbafkxtr 221 8 - - HYPH cord-275664-qbafkxtr 221 9 Rad Rad NNP cord-275664-qbafkxtr 221 10 protein protein NN cord-275664-qbafkxtr 221 11 assay assay NN cord-275664-qbafkxtr 221 12 ( ( -LRB- cord-275664-qbafkxtr 221 13 Bio Bio NNP cord-275664-qbafkxtr 221 14 - - HYPH cord-275664-qbafkxtr 221 15 Rad Rad NNP cord-275664-qbafkxtr 221 16 , , , cord-275664-qbafkxtr 221 17 Hercules Hercules NNP cord-275664-qbafkxtr 221 18 , , , cord-275664-qbafkxtr 221 19 CA CA NNP cord-275664-qbafkxtr 221 20 ) ) -RRB- cord-275664-qbafkxtr 221 21 with with IN cord-275664-qbafkxtr 221 22 BSA BSA NNP cord-275664-qbafkxtr 221 23 as as IN cord-275664-qbafkxtr 221 24 a a DT cord-275664-qbafkxtr 221 25 standard standard NN cord-275664-qbafkxtr 221 26 . . . cord-275664-qbafkxtr 222 1 The the DT cord-275664-qbafkxtr 222 2 whole whole JJ cord-275664-qbafkxtr 222 3 cell cell NN cord-275664-qbafkxtr 222 4 lysate lysate NN cord-275664-qbafkxtr 222 5 extracts extract NNS cord-275664-qbafkxtr 222 6 prepared prepare VBN cord-275664-qbafkxtr 222 7 as as IN cord-275664-qbafkxtr 222 8 described describe VBN cord-275664-qbafkxtr 222 9 above above RB cord-275664-qbafkxtr 222 10 were be VBD cord-275664-qbafkxtr 222 11 diluted dilute VBN cord-275664-qbafkxtr 222 12 in in IN cord-275664-qbafkxtr 222 13 2× 2× CD cord-275664-qbafkxtr 222 14 sample sample NN cord-275664-qbafkxtr 222 15 buffer buffer NN cord-275664-qbafkxtr 222 16 and and CC cord-275664-qbafkxtr 222 17 boiled boil VBN cord-275664-qbafkxtr 222 18 for for IN cord-275664-qbafkxtr 222 19 5 5 CD cord-275664-qbafkxtr 222 20 min min NN cord-275664-qbafkxtr 222 21 . . . cord-275664-qbafkxtr 223 1 Twenty twenty CD cord-275664-qbafkxtr 223 2 micrograms microgram NNS cord-275664-qbafkxtr 223 3 of of IN cord-275664-qbafkxtr 223 4 each each DT cord-275664-qbafkxtr 223 5 extract extract NN cord-275664-qbafkxtr 223 6 was be VBD cord-275664-qbafkxtr 223 7 resolved resolve VBN cord-275664-qbafkxtr 223 8 on on IN cord-275664-qbafkxtr 223 9 10 10 CD cord-275664-qbafkxtr 223 10 % % NN cord-275664-qbafkxtr 223 11 sodium sodium NN cord-275664-qbafkxtr 223 12 dodecyl dodecyl NNP cord-275664-qbafkxtr 223 13 sulphatepolyacrylamide sulphatepolyacrylamide NNP cord-275664-qbafkxtr 223 14 gel gel NN cord-275664-qbafkxtr 223 15 electrophoresis electrophoresis NN cord-275664-qbafkxtr 223 16 ( ( -LRB- cord-275664-qbafkxtr 223 17 SDS SDS NNP cord-275664-qbafkxtr 223 18 - - HYPH cord-275664-qbafkxtr 223 19 PAGE PAGE NNP cord-275664-qbafkxtr 223 20 ) ) -RRB- cord-275664-qbafkxtr 223 21 and and CC cord-275664-qbafkxtr 223 22 blotted blot VBN cord-275664-qbafkxtr 223 23 onto onto IN cord-275664-qbafkxtr 223 24 nitrocellulose nitrocellulose NN cord-275664-qbafkxtr 223 25 ( ( -LRB- cord-275664-qbafkxtr 223 26 NC NC NNP cord-275664-qbafkxtr 223 27 ) ) -RRB- cord-275664-qbafkxtr 223 28 membranes membrane NNS cord-275664-qbafkxtr 223 29 ( ( -LRB- cord-275664-qbafkxtr 223 30 Stratagene Stratagene NNP cord-275664-qbafkxtr 223 31 ) ) -RRB- cord-275664-qbafkxtr 223 32 with with IN cord-275664-qbafkxtr 223 33 a a DT cord-275664-qbafkxtr 223 34 semidry semidry JJ cord-275664-qbafkxtr 223 35 transfer transfer NN cord-275664-qbafkxtr 223 36 cell cell NN cord-275664-qbafkxtr 223 37 ( ( -LRB- cord-275664-qbafkxtr 223 38 Bio Bio NNP cord-275664-qbafkxtr 223 39 - - HYPH cord-275664-qbafkxtr 223 40 Rad Rad NNP cord-275664-qbafkxtr 223 41 Trans Trans NNP cord-275664-qbafkxtr 223 42 - - HYPH cord-275664-qbafkxtr 223 43 Blot Blot NNP cord-275664-qbafkxtr 223 44 SD SD NNP cord-275664-qbafkxtr 223 45 ) ) -RRB- cord-275664-qbafkxtr 223 46 . . . cord-275664-qbafkxtr 224 1 The the DT cord-275664-qbafkxtr 224 2 membranes membrane NNS cord-275664-qbafkxtr 224 3 were be VBD cord-275664-qbafkxtr 224 4 blocked block VBN cord-275664-qbafkxtr 224 5 for for IN cord-275664-qbafkxtr 224 6 2 2 CD cord-275664-qbafkxtr 224 7 h h NN cord-275664-qbafkxtr 224 8 at at IN cord-275664-qbafkxtr 224 9 RT RT NNP cord-275664-qbafkxtr 224 10 in in IN cord-275664-qbafkxtr 224 11 blocking block VBG cord-275664-qbafkxtr 224 12 buffer buffer NN cord-275664-qbafkxtr 224 13 TBST TBST NNP cord-275664-qbafkxtr 224 14 ( ( -LRB- cord-275664-qbafkxtr 224 15 20 20 CD cord-275664-qbafkxtr 224 16 mM mM NNP cord-275664-qbafkxtr 224 17 Tris Tris NNP cord-275664-qbafkxtr 224 18 - - HYPH cord-275664-qbafkxtr 224 19 HCl HCl NNP cord-275664-qbafkxtr 224 20 [ [ -LRB- cord-275664-qbafkxtr 224 21 pH pH NNP cord-275664-qbafkxtr 224 22 7.4 7.4 CD cord-275664-qbafkxtr 224 23 ] ] -RRB- cord-275664-qbafkxtr 224 24 , , , cord-275664-qbafkxtr 224 25 150 150 CD cord-275664-qbafkxtr 224 26 mM mm CD cord-275664-qbafkxtr 224 27 NaCl nacl NN cord-275664-qbafkxtr 224 28 , , , cord-275664-qbafkxtr 224 29 0.1 0.1 CD cord-275664-qbafkxtr 224 30 % % NN cord-275664-qbafkxtr 224 31 Tween-20 Tween-20 NNP cord-275664-qbafkxtr 224 32 ) ) -RRB- cord-275664-qbafkxtr 224 33 containing contain VBG cord-275664-qbafkxtr 224 34 5 5 CD cord-275664-qbafkxtr 224 35 % % NN cord-275664-qbafkxtr 224 36 skim skim NN cord-275664-qbafkxtr 224 37 milk milk NN cord-275664-qbafkxtr 224 38 powder powder NN cord-275664-qbafkxtr 224 39 to to TO cord-275664-qbafkxtr 224 40 prevent prevent VB cord-275664-qbafkxtr 224 41 nonspecific nonspecific JJ cord-275664-qbafkxtr 224 42 binding binding NN cord-275664-qbafkxtr 224 43 , , , cord-275664-qbafkxtr 224 44 and and CC cord-275664-qbafkxtr 224 45 then then RB cord-275664-qbafkxtr 224 46 incubated incubate VBN cord-275664-qbafkxtr 224 47 with with IN cord-275664-qbafkxtr 224 48 specific specific JJ cord-275664-qbafkxtr 224 49 primary primary JJ cord-275664-qbafkxtr 224 50 antibodies antibody NNS cord-275664-qbafkxtr 224 51 raised raise VBD cord-275664-qbafkxtr 224 52 against against IN cord-275664-qbafkxtr 224 53 ORF2 ORF2 NNP cord-275664-qbafkxtr 224 54 , , , cord-275664-qbafkxtr 224 55 ERK2 ERK2 NNP cord-275664-qbafkxtr 224 56 , , , cord-275664-qbafkxtr 224 57 phosphorylated phosphorylated JJ cord-275664-qbafkxtr 224 58 ( ( -LRB- cord-275664-qbafkxtr 224 59 p)-ERK1/2 p)-erk1/2 UH cord-275664-qbafkxtr 224 60 , , , cord-275664-qbafkxtr 224 61 and and CC cord-275664-qbafkxtr 224 62 ( ( -LRB- cord-275664-qbafkxtr 224 63 p)-ElK-1 p)-ElK-1 NNP cord-275664-qbafkxtr 224 64 , , , cord-275664-qbafkxtr 224 65 as as RB cord-275664-qbafkxtr 224 66 well well RB cord-275664-qbafkxtr 224 67 as as IN cord-275664-qbafkxtr 224 68 β β NN cord-275664-qbafkxtr 224 69 - - HYPH cord-275664-qbafkxtr 224 70 actin actin NN cord-275664-qbafkxtr 224 71 at at IN cord-275664-qbafkxtr 224 72 RT RT NNP cord-275664-qbafkxtr 224 73 for for IN cord-275664-qbafkxtr 224 74 2 2 CD cord-275664-qbafkxtr 224 75 h. h. NN cord-275664-qbafkxtr 225 1 The the DT cord-275664-qbafkxtr 225 2 membranes membrane NNS cord-275664-qbafkxtr 225 3 were be VBD cord-275664-qbafkxtr 225 4 washed wash VBN cord-275664-qbafkxtr 225 5 three three CD cord-275664-qbafkxtr 225 6 times time NNS cord-275664-qbafkxtr 225 7 with with IN cord-275664-qbafkxtr 225 8 TBST TBST NNP cord-275664-qbafkxtr 225 9 buffer buffer NN cord-275664-qbafkxtr 225 10 , , , cord-275664-qbafkxtr 225 11 and and CC cord-275664-qbafkxtr 225 12 incubated incubate VBN cord-275664-qbafkxtr 225 13 for for IN cord-275664-qbafkxtr 225 14 2 2 CD cord-275664-qbafkxtr 225 15 h h NN cord-275664-qbafkxtr 225 16 at at IN cord-275664-qbafkxtr 225 17 RT RT NNP cord-275664-qbafkxtr 225 18 with with IN cord-275664-qbafkxtr 225 19 HRP HRP NNP cord-275664-qbafkxtr 225 20 - - HYPH cord-275664-qbafkxtr 225 21 conjugated conjugate VBN cord-275664-qbafkxtr 225 22 secondary secondary JJ cord-275664-qbafkxtr 225 23 antibodies antibody NNS cord-275664-qbafkxtr 225 24 diluted dilute VBN cord-275664-qbafkxtr 225 25 in in IN cord-275664-qbafkxtr 225 26 blocking block VBG cord-275664-qbafkxtr 225 27 buffer buffer NN cord-275664-qbafkxtr 225 28 ( ( -LRB- cord-275664-qbafkxtr 225 29 1:2 1:2 CD cord-275664-qbafkxtr 225 30 000 000 CD cord-275664-qbafkxtr 225 31 ) ) -RRB- cord-275664-qbafkxtr 225 32 . . . cord-275664-qbafkxtr 226 1 Immunoreactive Immunoreactive NNP cord-275664-qbafkxtr 226 2 bands band NNS cord-275664-qbafkxtr 226 3 were be VBD cord-275664-qbafkxtr 226 4 visualized visualize VBN cord-275664-qbafkxtr 226 5 by by IN cord-275664-qbafkxtr 226 6 enhanced enhance VBN cord-275664-qbafkxtr 226 7 chemiluminescence chemiluminescence NN cord-275664-qbafkxtr 226 8 system system NN cord-275664-qbafkxtr 226 9 ( ( -LRB- cord-275664-qbafkxtr 226 10 Amersham Amersham NNP cord-275664-qbafkxtr 226 11 Biosciences Biosciences NNP cord-275664-qbafkxtr 226 12 ) ) -RRB- cord-275664-qbafkxtr 226 13 . . . cord-275664-qbafkxtr 227 1 Results result NNS cord-275664-qbafkxtr 227 2 are be VBP cord-275664-qbafkxtr 227 3 presented present VBN cord-275664-qbafkxtr 227 4 as as IN cord-275664-qbafkxtr 227 5 averages average NNS cord-275664-qbafkxtr 227 6 ± ± NNP cord-275664-qbafkxtr 227 7 the the DT cord-275664-qbafkxtr 227 8 standard standard JJ cord-275664-qbafkxtr 227 9 deviations deviation NNS cord-275664-qbafkxtr 227 10 or or CC cord-275664-qbafkxtr 227 11 standard standard JJ cord-275664-qbafkxtr 227 12 errors error NNS cord-275664-qbafkxtr 227 13 of of IN cord-275664-qbafkxtr 227 14 the the DT cord-275664-qbafkxtr 227 15 means mean NNS cord-275664-qbafkxtr 227 16 , , , cord-275664-qbafkxtr 227 17 as as IN cord-275664-qbafkxtr 227 18 indicated indicate VBN cord-275664-qbafkxtr 227 19 . . . cord-275664-qbafkxtr 228 1 Statistical statistical JJ cord-275664-qbafkxtr 228 2 comparisons comparison NNS cord-275664-qbafkxtr 228 3 are be VBP cord-275664-qbafkxtr 228 4 made make VBN cord-275664-qbafkxtr 228 5 by by IN cord-275664-qbafkxtr 228 6 using use VBG cord-275664-qbafkxtr 228 7 Student Student NNP cord-275664-qbafkxtr 228 8 's 's POS cord-275664-qbafkxtr 228 9 t t NN cord-275664-qbafkxtr 228 10 test test NN cord-275664-qbafkxtr 228 11 , , , cord-275664-qbafkxtr 228 12 and and CC cord-275664-qbafkxtr 228 13 differences difference NNS cord-275664-qbafkxtr 228 14 between between IN cord-275664-qbafkxtr 228 15 groups group NNS cord-275664-qbafkxtr 228 16 were be VBD cord-275664-qbafkxtr 228 17 considered consider VBN cord-275664-qbafkxtr 228 18 significant significant JJ cord-275664-qbafkxtr 228 19 if if IN cord-275664-qbafkxtr 228 20 the the DT cord-275664-qbafkxtr 228 21 P p NN cord-275664-qbafkxtr 228 22 value value NN cord-275664-qbafkxtr 228 23 was be VBD cord-275664-qbafkxtr 228 24 b b NN cord-275664-qbafkxtr 228 25 0.05 0.05 CD cord-275664-qbafkxtr 228 26 . . . cord-275664-qbafkxtr 229 1 Porcine porcine JJ cord-275664-qbafkxtr 229 2 circoviruses circoviruse NNS cord-275664-qbafkxtr 229 3 : : : cord-275664-qbafkxtr 230 1 a a DT cord-275664-qbafkxtr 230 2 review review NN cord-275664-qbafkxtr 230 3 Pathogenesis Pathogenesis NNP cord-275664-qbafkxtr 230 4 of of IN cord-275664-qbafkxtr 230 5 porcine porcine JJ cord-275664-qbafkxtr 230 6 circovirus circovirus NNP cord-275664-qbafkxtr 230 7 : : : cord-275664-qbafkxtr 230 8 experimental experimental JJ cord-275664-qbafkxtr 230 9 infections infection NNS cord-275664-qbafkxtr 230 10 of of IN cord-275664-qbafkxtr 230 11 colostrum colostrum NN cord-275664-qbafkxtr 230 12 deprived deprive VBD cord-275664-qbafkxtr 230 13 piglets piglet NNS cord-275664-qbafkxtr 230 14 and and CC cord-275664-qbafkxtr 230 15 examination examination NN cord-275664-qbafkxtr 230 16 of of IN cord-275664-qbafkxtr 230 17 pig pig NN cord-275664-qbafkxtr 230 18 foetal foetal JJ cord-275664-qbafkxtr 230 19 material material NN cord-275664-qbafkxtr 230 20 Isolation isolation NN cord-275664-qbafkxtr 230 21 of of IN cord-275664-qbafkxtr 230 22 porcine porcine JJ cord-275664-qbafkxtr 230 23 circovirus circovirus JJ cord-275664-qbafkxtr 230 24 - - HYPH cord-275664-qbafkxtr 230 25 like like JJ cord-275664-qbafkxtr 230 26 viruses virus NNS cord-275664-qbafkxtr 230 27 from from IN cord-275664-qbafkxtr 230 28 pigs pig NNS cord-275664-qbafkxtr 230 29 with with IN cord-275664-qbafkxtr 230 30 a a DT cord-275664-qbafkxtr 230 31 wasting waste VBG cord-275664-qbafkxtr 230 32 disease disease NN cord-275664-qbafkxtr 230 33 in in IN cord-275664-qbafkxtr 230 34 the the DT cord-275664-qbafkxtr 230 35 USA USA NNP cord-275664-qbafkxtr 230 36 and and CC cord-275664-qbafkxtr 230 37 Europe Europe NNP cord-275664-qbafkxtr 231 1 The the DT cord-275664-qbafkxtr 231 2 vaccinia vaccinia NN cord-275664-qbafkxtr 231 3 virus virus NN cord-275664-qbafkxtr 231 4 - - HYPH cord-275664-qbafkxtr 231 5 stimulated stimulate VBN cord-275664-qbafkxtr 231 6 mitogen mitogen NN cord-275664-qbafkxtr 231 7 - - HYPH cord-275664-qbafkxtr 231 8 activated activate VBN cord-275664-qbafkxtr 231 9 protein protein NN cord-275664-qbafkxtr 231 10 kinase kinase NN cord-275664-qbafkxtr 231 11 ( ( -LRB- cord-275664-qbafkxtr 231 12 MAPK MAPK NNP cord-275664-qbafkxtr 231 13 ) ) -RRB- cord-275664-qbafkxtr 231 14 pathway pathway NN cord-275664-qbafkxtr 231 15 is be VBZ cord-275664-qbafkxtr 231 16 required require VBN cord-275664-qbafkxtr 231 17 for for IN cord-275664-qbafkxtr 231 18 virus virus NN cord-275664-qbafkxtr 231 19 multiplication multiplication NN cord-275664-qbafkxtr 231 20 Visna Visna NNP cord-275664-qbafkxtr 231 21 virus virus NN cord-275664-qbafkxtr 231 22 - - HYPH cord-275664-qbafkxtr 231 23 induced induce VBN cord-275664-qbafkxtr 231 24 activation activation NN cord-275664-qbafkxtr 231 25 of of IN cord-275664-qbafkxtr 231 26 MAPKs mapk NNS cord-275664-qbafkxtr 231 27 is be VBZ cord-275664-qbafkxtr 231 28 required require VBN cord-275664-qbafkxtr 231 29 for for IN cord-275664-qbafkxtr 231 30 virus virus NN cord-275664-qbafkxtr 231 31 replication replication NN cord-275664-qbafkxtr 231 32 and and CC cord-275664-qbafkxtr 231 33 correlates correlate VBZ cord-275664-qbafkxtr 231 34 with with IN cord-275664-qbafkxtr 231 35 virus virus NN cord-275664-qbafkxtr 231 36 - - HYPH cord-275664-qbafkxtr 231 37 induced induce VBN cord-275664-qbafkxtr 231 38 neuropathology neuropathology NN cord-275664-qbafkxtr 231 39 Suppression suppression NN cord-275664-qbafkxtr 231 40 of of IN cord-275664-qbafkxtr 231 41 coronavirus coronavirus NN cord-275664-qbafkxtr 231 42 replication replication NN cord-275664-qbafkxtr 231 43 by by IN cord-275664-qbafkxtr 231 44 inhibition inhibition NN cord-275664-qbafkxtr 231 45 of of IN cord-275664-qbafkxtr 231 46 the the DT cord-275664-qbafkxtr 231 47 MEK MEK NNP cord-275664-qbafkxtr 231 48 signaling signal VBG cord-275664-qbafkxtr 231 49 pathway pathway NN cord-275664-qbafkxtr 231 50 Transcriptional Transcriptional NNP cord-275664-qbafkxtr 231 51 analysis analysis NN cord-275664-qbafkxtr 231 52 of of IN cord-275664-qbafkxtr 231 53 porcine porcine JJ cord-275664-qbafkxtr 231 54 circovirus circovirus JJ cord-275664-qbafkxtr 231 55 type type NN cord-275664-qbafkxtr 231 56 2 2 CD cord-275664-qbafkxtr 231 57 Rolling rolling NN cord-275664-qbafkxtr 231 58 - - HYPH cord-275664-qbafkxtr 231 59 circle circle NN cord-275664-qbafkxtr 231 60 replication replication NN cord-275664-qbafkxtr 231 61 of of IN cord-275664-qbafkxtr 231 62 an an DT cord-275664-qbafkxtr 231 63 animal animal NN cord-275664-qbafkxtr 231 64 circovirus circovirus NN cord-275664-qbafkxtr 231 65 genome genome NN cord-275664-qbafkxtr 231 66 in in IN cord-275664-qbafkxtr 231 67 a a DT cord-275664-qbafkxtr 231 68 thetareplicating thetareplicating JJ cord-275664-qbafkxtr 231 69 bacterial bacterial JJ cord-275664-qbafkxtr 231 70 plasmid plasmid NN cord-275664-qbafkxtr 231 71 in in IN cord-275664-qbafkxtr 231 72 Escherichia Escherichia NNP cord-275664-qbafkxtr 231 73 coli coli VBZ cord-275664-qbafkxtr 231 74 Porcine porcine JJ cord-275664-qbafkxtr 231 75 postweaning postweane VBG cord-275664-qbafkxtr 231 76 multisystemic multisystemic NNP cord-275664-qbafkxtr 231 77 wasting waste VBG cord-275664-qbafkxtr 231 78 syndrome syndrome NN cord-275664-qbafkxtr 231 79 in in IN cord-275664-qbafkxtr 231 80 Korean korean JJ cord-275664-qbafkxtr 231 81 pig pig NN cord-275664-qbafkxtr 231 82 : : : cord-275664-qbafkxtr 231 83 detection detection NN cord-275664-qbafkxtr 231 84 of of IN cord-275664-qbafkxtr 231 85 porcine porcine JJ cord-275664-qbafkxtr 231 86 circovirus circovirus NNP cord-275664-qbafkxtr 231 87 2 2 CD cord-275664-qbafkxtr 231 88 infection infection NN cord-275664-qbafkxtr 231 89 by by IN cord-275664-qbafkxtr 231 90 immunohistochemistry immunohistochemistry NN cord-275664-qbafkxtr 231 91 and and CC cord-275664-qbafkxtr 231 92 polymerase polymerase NN cord-275664-qbafkxtr 231 93 chain chain NN cord-275664-qbafkxtr 231 94 reaction reaction NN cord-275664-qbafkxtr 231 95 Post Post NNP cord-275664-qbafkxtr 231 96 - - HYPH cord-275664-qbafkxtr 231 97 weaning wean VBG cord-275664-qbafkxtr 231 98 multisystemic multisystemic NNP cord-275664-qbafkxtr 232 1 wasting waste VBG cord-275664-qbafkxtr 232 2 syndrome syndrome NN cord-275664-qbafkxtr 232 3 Pathogenesis Pathogenesis NNP cord-275664-qbafkxtr 232 4 of of IN cord-275664-qbafkxtr 232 5 postweaning postweane VBG cord-275664-qbafkxtr 232 6 multisystemic multisystemic NNP cord-275664-qbafkxtr 232 7 wasting waste VBG cord-275664-qbafkxtr 232 8 syndrome syndrome NN cord-275664-qbafkxtr 232 9 caused cause VBN cord-275664-qbafkxtr 232 10 by by IN cord-275664-qbafkxtr 232 11 porcine porcine JJ cord-275664-qbafkxtr 232 12 circovirus circovirus NNP cord-275664-qbafkxtr 232 13 2 2 CD cord-275664-qbafkxtr 232 14 : : : cord-275664-qbafkxtr 232 15 an an DT cord-275664-qbafkxtr 232 16 immune immune JJ cord-275664-qbafkxtr 232 17 riddle riddle NN cord-275664-qbafkxtr 233 1 A a DT cord-275664-qbafkxtr 233 2 mitogenic mitogenic JJ cord-275664-qbafkxtr 233 3 signal signal NN cord-275664-qbafkxtr 233 4 triggered trigger VBN cord-275664-qbafkxtr 233 5 at at IN cord-275664-qbafkxtr 233 6 an an DT cord-275664-qbafkxtr 233 7 early early JJ cord-275664-qbafkxtr 233 8 stage stage NN cord-275664-qbafkxtr 233 9 of of IN cord-275664-qbafkxtr 233 10 vaccinia vaccinia NN cord-275664-qbafkxtr 233 11 virus virus NN cord-275664-qbafkxtr 233 12 infection infection NN cord-275664-qbafkxtr 233 13 : : : cord-275664-qbafkxtr 233 14 implication implication NN cord-275664-qbafkxtr 233 15 of of IN cord-275664-qbafkxtr 233 16 MEK MEK NNP cord-275664-qbafkxtr 233 17 / / SYM cord-275664-qbafkxtr 233 18 ERK ERK NNP cord-275664-qbafkxtr 233 19 and and CC cord-275664-qbafkxtr 233 20 protein protein NN cord-275664-qbafkxtr 233 21 kinase kinase VBZ cord-275664-qbafkxtr 233 22 A a NN cord-275664-qbafkxtr 233 23 in in IN cord-275664-qbafkxtr 233 24 virus virus NN cord-275664-qbafkxtr 233 25 multiplication multiplication NN cord-275664-qbafkxtr 233 26 Evidence evidence NN cord-275664-qbafkxtr 233 27 of of IN cord-275664-qbafkxtr 233 28 circovirus circovirus JJ cord-275664-qbafkxtr 233 29 infection infection NN cord-275664-qbafkxtr 233 30 in in IN cord-275664-qbafkxtr 233 31 British british JJ cord-275664-qbafkxtr 233 32 pigs pig NNS cord-275664-qbafkxtr 233 33 Genetic genetic JJ cord-275664-qbafkxtr 233 34 characterization characterization NN cord-275664-qbafkxtr 233 35 of of IN cord-275664-qbafkxtr 233 36 type type NN cord-275664-qbafkxtr 233 37 2 2 CD cord-275664-qbafkxtr 233 38 porcine porcine JJ cord-275664-qbafkxtr 233 39 circovirus circovirus NNP cord-275664-qbafkxtr 233 40 ( ( -LRB- cord-275664-qbafkxtr 233 41 PCV-2 PCV-2 NNP cord-275664-qbafkxtr 233 42 ) ) -RRB- cord-275664-qbafkxtr 233 43 from from IN cord-275664-qbafkxtr 233 44 pigs pig NNS cord-275664-qbafkxtr 233 45 with with IN cord-275664-qbafkxtr 233 46 postweaning postweane VBG cord-275664-qbafkxtr 233 47 multisystemic multisystemic NNP cord-275664-qbafkxtr 233 48 wasting waste VBG cord-275664-qbafkxtr 233 49 syndrome syndrome NN cord-275664-qbafkxtr 233 50 in in IN cord-275664-qbafkxtr 233 51 different different JJ cord-275664-qbafkxtr 233 52 geographic geographic JJ cord-275664-qbafkxtr 233 53 regions region NNS cord-275664-qbafkxtr 233 54 of of IN cord-275664-qbafkxtr 233 55 North North NNP cord-275664-qbafkxtr 233 56 America America NNP cord-275664-qbafkxtr 233 57 and and CC cord-275664-qbafkxtr 233 58 development development NN cord-275664-qbafkxtr 233 59 of of IN cord-275664-qbafkxtr 233 60 a a DT cord-275664-qbafkxtr 233 61 differential differential JJ cord-275664-qbafkxtr 233 62 PCR pcr NN cord-275664-qbafkxtr 233 63 - - HYPH cord-275664-qbafkxtr 233 64 restriction restriction NN cord-275664-qbafkxtr 233 65 fragment fragment NN cord-275664-qbafkxtr 233 66 length length NN cord-275664-qbafkxtr 233 67 polymorphism polymorphism NN cord-275664-qbafkxtr 233 68 assay assay NN cord-275664-qbafkxtr 233 69 to to TO cord-275664-qbafkxtr 233 70 detect detect VB cord-275664-qbafkxtr 233 71 and and CC cord-275664-qbafkxtr 233 72 differentiate differentiate VB cord-275664-qbafkxtr 233 73 between between IN cord-275664-qbafkxtr 233 74 infections infection NNS cord-275664-qbafkxtr 233 75 with with IN cord-275664-qbafkxtr 233 76 PCV-1 PCV-1 NNP cord-275664-qbafkxtr 233 77 and and CC cord-275664-qbafkxtr 233 78 PCV-2 PCV-2 NNP cord-275664-qbafkxtr 233 79 Organization Organization NNP cord-275664-qbafkxtr 233 80 and and CC cord-275664-qbafkxtr 233 81 regulation regulation NN cord-275664-qbafkxtr 233 82 of of IN cord-275664-qbafkxtr 233 83 mitogen mitogen NN cord-275664-qbafkxtr 233 84 - - HYPH cord-275664-qbafkxtr 233 85 activated activate VBN cord-275664-qbafkxtr 233 86 protein protein NN cord-275664-qbafkxtr 233 87 kinase kinase NN cord-275664-qbafkxtr 233 88 signaling signal VBG cord-275664-qbafkxtr 233 89 pathways pathway NNS cord-275664-qbafkxtr 234 1 Post post JJ cord-275664-qbafkxtr 234 2 - - JJ cord-275664-qbafkxtr 234 3 weaning wean VBG cord-275664-qbafkxtr 234 4 multisystemic multisystemic NNP cord-275664-qbafkxtr 234 5 wasting waste VBG cord-275664-qbafkxtr 234 6 syndrome syndrome NN cord-275664-qbafkxtr 234 7 ( ( -LRB- cord-275664-qbafkxtr 234 8 PMWS PMWS NNP cord-275664-qbafkxtr 234 9 ) ) -RRB- cord-275664-qbafkxtr 234 10 : : : cord-275664-qbafkxtr 234 11 preliminary preliminary JJ cord-275664-qbafkxtr 234 12 epidemiology epidemiology NN cord-275664-qbafkxtr 234 13 and and CC cord-275664-qbafkxtr 234 14 clinical clinical JJ cord-275664-qbafkxtr 234 15 presentation presentation NN cord-275664-qbafkxtr 235 1 The the DT cord-275664-qbafkxtr 235 2 role role NN cord-275664-qbafkxtr 235 3 of of IN cord-275664-qbafkxtr 235 4 MKK1/2 MKK1/2 NNP cord-275664-qbafkxtr 235 5 kinase kinase NN cord-275664-qbafkxtr 235 6 activity activity NN cord-275664-qbafkxtr 235 7 in in IN cord-275664-qbafkxtr 235 8 human human JJ cord-275664-qbafkxtr 235 9 cytomegalovirus cytomegalovirus NN cord-275664-qbafkxtr 235 10 infection infection NN cord-275664-qbafkxtr 235 11 ERK-1/2 erk-1/2 IN cord-275664-qbafkxtr 235 12 activity activity NN cord-275664-qbafkxtr 235 13 is be VBZ cord-275664-qbafkxtr 235 14 required require VBN cord-275664-qbafkxtr 235 15 for for IN cord-275664-qbafkxtr 235 16 RSV RSV NNP cord-275664-qbafkxtr 235 17 efficient efficient JJ cord-275664-qbafkxtr 235 18 infection infection NN cord-275664-qbafkxtr 235 19 Human human JJ cord-275664-qbafkxtr 235 20 immunodeficiency immunodeficiency NN cord-275664-qbafkxtr 235 21 virus virus NN cord-275664-qbafkxtr 235 22 type type NN cord-275664-qbafkxtr 235 23 1 1 CD cord-275664-qbafkxtr 235 24 enters enter VBZ cord-275664-qbafkxtr 235 25 brain brain NN cord-275664-qbafkxtr 235 26 microvascular microvascular JJ cord-275664-qbafkxtr 235 27 endothelia endothelia NNP cord-275664-qbafkxtr 235 28 by by IN cord-275664-qbafkxtr 235 29 macropinocytosis macropinocytosis NNP cord-275664-qbafkxtr 235 30 dependent dependent JJ cord-275664-qbafkxtr 235 31 on on IN cord-275664-qbafkxtr 235 32 lipid lipid NN cord-275664-qbafkxtr 235 33 rafts raft NNS cord-275664-qbafkxtr 235 34 and and CC cord-275664-qbafkxtr 235 35 mitogen mitogen NN cord-275664-qbafkxtr 235 36 - - HYPH cord-275664-qbafkxtr 235 37 activated activate VBN cord-275664-qbafkxtr 235 38 protein protein NN cord-275664-qbafkxtr 235 39 kinase kinase NN cord-275664-qbafkxtr 235 40 signaling signal VBG cord-275664-qbafkxtr 235 41 pathway pathway NN cord-275664-qbafkxtr 235 42 Characterization characterization NN cord-275664-qbafkxtr 235 43 of of IN cord-275664-qbafkxtr 235 44 a a DT cord-275664-qbafkxtr 235 45 previously previously RB cord-275664-qbafkxtr 235 46 unidentified unidentified JJ cord-275664-qbafkxtr 235 47 viral viral JJ cord-275664-qbafkxtr 235 48 protein protein NN cord-275664-qbafkxtr 235 49 of of IN cord-275664-qbafkxtr 235 50 porcine porcine JJ cord-275664-qbafkxtr 235 51 circovirus circovirus NNP cord-275664-qbafkxtr 235 52 type type NN cord-275664-qbafkxtr 235 53 2-infected 2-infected CD cord-275664-qbafkxtr 235 54 cells cell NNS cord-275664-qbafkxtr 235 55 and and CC cord-275664-qbafkxtr 235 56 its -PRON- PRP$ cord-275664-qbafkxtr 235 57 role role NN cord-275664-qbafkxtr 235 58 in in IN cord-275664-qbafkxtr 235 59 virus virus NN cord-275664-qbafkxtr 235 60 - - HYPH cord-275664-qbafkxtr 235 61 induced induce VBN cord-275664-qbafkxtr 235 62 apoptosis apoptosis NN cord-275664-qbafkxtr 236 1 The the DT cord-275664-qbafkxtr 236 2 ORF3 ORF3 NNP cord-275664-qbafkxtr 236 3 protein protein NN cord-275664-qbafkxtr 236 4 of of IN cord-275664-qbafkxtr 236 5 porcine porcine JJ cord-275664-qbafkxtr 236 6 circovirus circovirus JJ cord-275664-qbafkxtr 236 7 type type NN cord-275664-qbafkxtr 236 8 2 2 CD cord-275664-qbafkxtr 236 9 is be VBZ cord-275664-qbafkxtr 236 10 involved involve VBN cord-275664-qbafkxtr 236 11 in in IN cord-275664-qbafkxtr 236 12 viral viral JJ cord-275664-qbafkxtr 236 13 pathogenesis pathogenesis NN cord-275664-qbafkxtr 236 14 in in IN cord-275664-qbafkxtr 236 15 vivo vivo NNP cord-275664-qbafkxtr 236 16 Coxsackievirus Coxsackievirus NNP cord-275664-qbafkxtr 236 17 B3 b3 NN cord-275664-qbafkxtr 236 18 replication replication NN cord-275664-qbafkxtr 236 19 is be VBZ cord-275664-qbafkxtr 236 20 reduced reduce VBN cord-275664-qbafkxtr 236 21 by by IN cord-275664-qbafkxtr 236 22 inhibition inhibition NN cord-275664-qbafkxtr 236 23 of of IN cord-275664-qbafkxtr 236 24 the the DT cord-275664-qbafkxtr 236 25 extracellular extracellular JJ cord-275664-qbafkxtr 236 26 signal signal NN cord-275664-qbafkxtr 236 27 - - HYPH cord-275664-qbafkxtr 236 28 regulated regulate VBN cord-275664-qbafkxtr 236 29 kinase kinase NN cord-275664-qbafkxtr 236 30 ( ( -LRB- cord-275664-qbafkxtr 236 31 ERK ERK NNP cord-275664-qbafkxtr 236 32 ) ) -RRB- cord-275664-qbafkxtr 236 33 signaling signal VBG cord-275664-qbafkxtr 236 34 pathway pathway NN cord-275664-qbafkxtr 236 35 Location location NN cord-275664-qbafkxtr 236 36 , , , cord-275664-qbafkxtr 236 37 location location NN cord-275664-qbafkxtr 236 38 , , , cord-275664-qbafkxtr 236 39 location location NN cord-275664-qbafkxtr 236 40 " " '' cord-275664-qbafkxtr 236 41 : : : cord-275664-qbafkxtr 236 42 activation activation NN cord-275664-qbafkxtr 236 43 and and CC cord-275664-qbafkxtr 236 44 targeting targeting NN cord-275664-qbafkxtr 236 45 of of IN cord-275664-qbafkxtr 236 46 MAP MAP NNP cord-275664-qbafkxtr 236 47 kinases kinase NNS cord-275664-qbafkxtr 236 48 by by IN cord-275664-qbafkxtr 236 49 G g NN cord-275664-qbafkxtr 236 50 protein protein NN cord-275664-qbafkxtr 236 51 - - HYPH cord-275664-qbafkxtr 236 52 coupled couple VBN cord-275664-qbafkxtr 236 53 receptors receptor NNS cord-275664-qbafkxtr 236 54 Identification identification NN cord-275664-qbafkxtr 236 55 of of IN cord-275664-qbafkxtr 236 56 a a DT cord-275664-qbafkxtr 236 57 protein protein NN cord-275664-qbafkxtr 236 58 essential essential JJ cord-275664-qbafkxtr 236 59 for for IN cord-275664-qbafkxtr 236 60 replication replication NN cord-275664-qbafkxtr 236 61 of of IN cord-275664-qbafkxtr 236 62 porcine porcine JJ cord-275664-qbafkxtr 236 63 circovirus circovirus JJ cord-275664-qbafkxtr 236 64 Suppression Suppression NNP cord-275664-qbafkxtr 236 65 of of IN cord-275664-qbafkxtr 236 66 astrovirus astrovirus NNP cord-275664-qbafkxtr 236 67 replication replication NN cord-275664-qbafkxtr 236 68 by by IN cord-275664-qbafkxtr 236 69 an an DT cord-275664-qbafkxtr 236 70 ERK1/2 ERK1/2 NNP cord-275664-qbafkxtr 236 71 inhibition inhibition NN cord-275664-qbafkxtr 237 1 Kaposi Kaposi NNP cord-275664-qbafkxtr 237 2 's 's POS cord-275664-qbafkxtr 237 3 sarcomaassociated sarcomaassociate VBN cord-275664-qbafkxtr 237 4 herpesvirus herpesvirus NN cord-275664-qbafkxtr 237 5 induces induce VBZ cord-275664-qbafkxtr 237 6 the the DT cord-275664-qbafkxtr 237 7 phosphatidylinositol phosphatidylinositol JJ cord-275664-qbafkxtr 237 8 3-kinase 3-kinase NNP cord-275664-qbafkxtr 237 9 - - HYPH cord-275664-qbafkxtr 237 10 PKC PKC NNP cord-275664-qbafkxtr 237 11 - - HYPH cord-275664-qbafkxtr 237 12 ζ ζ NNP cord-275664-qbafkxtr 237 13 - - HYPH cord-275664-qbafkxtr 237 14 NEK NEK NNP cord-275664-qbafkxtr 237 15 - - HYPH cord-275664-qbafkxtr 237 16 ERK ERK NNP cord-275664-qbafkxtr 237 17 signaling signal VBG cord-275664-qbafkxtr 237 18 pathway pathway NN cord-275664-qbafkxtr 237 19 in in IN cord-275664-qbafkxtr 237 20 target target NN cord-275664-qbafkxtr 237 21 cells cell NNS cord-275664-qbafkxtr 237 22 early early RB cord-275664-qbafkxtr 237 23 during during IN cord-275664-qbafkxtr 237 24 infection infection NN cord-275664-qbafkxtr 237 25 : : : cord-275664-qbafkxtr 237 26 implications implication NNS cord-275664-qbafkxtr 237 27 for for IN cord-275664-qbafkxtr 237 28 infectivity infectivity NN cord-275664-qbafkxtr 237 29 Open open JJ cord-275664-qbafkxtr 237 30 reading reading NN cord-275664-qbafkxtr 237 31 frame frame NN cord-275664-qbafkxtr 237 32 2 2 CD cord-275664-qbafkxtr 237 33 of of IN cord-275664-qbafkxtr 237 34 porcine porcine JJ cord-275664-qbafkxtr 237 35 circovirus circovirus NN cord-275664-qbafkxtr 237 36 type type NN cord-275664-qbafkxtr 237 37 2 2 CD cord-275664-qbafkxtr 237 38 encodes encode VBZ cord-275664-qbafkxtr 237 39 a a DT cord-275664-qbafkxtr 237 40 major major JJ cord-275664-qbafkxtr 237 41 capsid capsid NN cord-275664-qbafkxtr 237 42 protein protein NN cord-275664-qbafkxtr 237 43 Detection detection NN cord-275664-qbafkxtr 237 44 of of IN cord-275664-qbafkxtr 237 45 porcine porcine JJ cord-275664-qbafkxtr 237 46 circovirus circovirus NN cord-275664-qbafkxtr 237 47 from from IN cord-275664-qbafkxtr 237 48 lesions lesion NNS cord-275664-qbafkxtr 237 49 of of IN cord-275664-qbafkxtr 237 50 a a DT cord-275664-qbafkxtr 237 51 pig pig NN cord-275664-qbafkxtr 237 52 with with IN cord-275664-qbafkxtr 237 53 wasting waste VBG cord-275664-qbafkxtr 237 54 disease disease NN cord-275664-qbafkxtr 237 55 in in IN cord-275664-qbafkxtr 237 56 Japan Japan NNP cord-275664-qbafkxtr 238 1 The the DT cord-275664-qbafkxtr 238 2 herpes herpe NNS cord-275664-qbafkxtr 238 3 simplex simplex NN cord-275664-qbafkxtr 238 4 virus virus NN cord-275664-qbafkxtr 238 5 type type NN cord-275664-qbafkxtr 238 6 2 2 CD cord-275664-qbafkxtr 238 7 R1 r1 NN cord-275664-qbafkxtr 238 8 protein protein NN cord-275664-qbafkxtr 238 9 kinase kinase NN cord-275664-qbafkxtr 238 10 ( ( -LRB- cord-275664-qbafkxtr 238 11 ICP10 icp10 NN cord-275664-qbafkxtr 238 12 PK PK NNP cord-275664-qbafkxtr 238 13 ) ) -RRB- cord-275664-qbafkxtr 238 14 blocks block VBZ cord-275664-qbafkxtr 238 15 apoptosis apoptosis NN cord-275664-qbafkxtr 238 16 in in IN cord-275664-qbafkxtr 238 17 hippocampal hippocampal JJ cord-275664-qbafkxtr 238 18 neurons neuron NNS cord-275664-qbafkxtr 238 19 , , , cord-275664-qbafkxtr 238 20 involving involve VBG cord-275664-qbafkxtr 238 21 activation activation NN cord-275664-qbafkxtr 238 22 of of IN cord-275664-qbafkxtr 238 23 the the DT cord-275664-qbafkxtr 238 24 MEK MEK NNP cord-275664-qbafkxtr 238 25 / / SYM cord-275664-qbafkxtr 238 26 MAPK MAPK NNP cord-275664-qbafkxtr 238 27 survival survival NN cord-275664-qbafkxtr 238 28 pathway pathway NN cord-275664-qbafkxtr 238 29 MEK mek NN cord-275664-qbafkxtr 238 30 - - HYPH cord-275664-qbafkxtr 238 31 specific specific JJ cord-275664-qbafkxtr 238 32 inhibitor inhibitor NN cord-275664-qbafkxtr 238 33 U0126 U0126 NNP cord-275664-qbafkxtr 238 34 blocks block NNS cord-275664-qbafkxtr 238 35 spread spread NN cord-275664-qbafkxtr 238 36 of of IN cord-275664-qbafkxtr 238 37 Borna Borna NNP cord-275664-qbafkxtr 238 38 disease disease NN cord-275664-qbafkxtr 238 39 virus virus NN cord-275664-qbafkxtr 238 40 in in IN cord-275664-qbafkxtr 238 41 cultured cultured JJ cord-275664-qbafkxtr 238 42 cells cell NNS cord-275664-qbafkxtr 239 1 Influenza influenza NN cord-275664-qbafkxtr 239 2 virus virus NN cord-275664-qbafkxtr 239 3 propagation propagation NN cord-275664-qbafkxtr 239 4 is be VBZ cord-275664-qbafkxtr 239 5 impaired impair VBN cord-275664-qbafkxtr 239 6 by by IN cord-275664-qbafkxtr 239 7 inhibition inhibition NN cord-275664-qbafkxtr 239 8 of of IN cord-275664-qbafkxtr 239 9 the the DT cord-275664-qbafkxtr 239 10 Raf Raf NNP cord-275664-qbafkxtr 239 11 / / SYM cord-275664-qbafkxtr 239 12 MEK MEK NNP cord-275664-qbafkxtr 239 13 / / SYM cord-275664-qbafkxtr 239 14 ERK ERK NNP cord-275664-qbafkxtr 239 15 signalling signal VBG cord-275664-qbafkxtr 239 16 cascade cascade NN cord-275664-qbafkxtr 239 17 Risk risk NN cord-275664-qbafkxtr 239 18 factors factor NNS cord-275664-qbafkxtr 239 19 for for IN cord-275664-qbafkxtr 239 20 porcine porcine JJ cord-275664-qbafkxtr 239 21 post post JJ cord-275664-qbafkxtr 239 22 - - JJ cord-275664-qbafkxtr 239 23 weaning wean VBG cord-275664-qbafkxtr 239 24 multisystemic multisystemic NNP cord-275664-qbafkxtr 239 25 wasting waste VBG cord-275664-qbafkxtr 239 26 syndrome syndrome NN cord-275664-qbafkxtr 239 27 ( ( -LRB- cord-275664-qbafkxtr 239 28 PMWS PMWS NNP cord-275664-qbafkxtr 239 29 ) ) -RRB- cord-275664-qbafkxtr 239 30 in in IN cord-275664-qbafkxtr 239 31 149 149 CD cord-275664-qbafkxtr 239 32 French french JJ cord-275664-qbafkxtr 239 33 farrow farrow NN cord-275664-qbafkxtr 239 34 - - HYPH cord-275664-qbafkxtr 239 35 to to IN cord-275664-qbafkxtr 239 36 - - HYPH cord-275664-qbafkxtr 239 37 finish finish NN cord-275664-qbafkxtr 239 38 herds herd NNS cord-275664-qbafkxtr 239 39 ERK ERK NNP cord-275664-qbafkxtr 239 40 and and CC cord-275664-qbafkxtr 239 41 p38 p38 CD cord-275664-qbafkxtr 239 42 MAPK MAPK NNP cord-275664-qbafkxtr 239 43 - - HYPH cord-275664-qbafkxtr 239 44 activated activate VBN cord-275664-qbafkxtr 239 45 protein protein NN cord-275664-qbafkxtr 239 46 kinases kinase NNS cord-275664-qbafkxtr 239 47 : : : cord-275664-qbafkxtr 239 48 a a DT cord-275664-qbafkxtr 239 49 family family NN cord-275664-qbafkxtr 239 50 of of IN cord-275664-qbafkxtr 239 51 protein protein NN cord-275664-qbafkxtr 239 52 kinases kinase NNS cord-275664-qbafkxtr 239 53 with with IN cord-275664-qbafkxtr 239 54 diverse diverse JJ cord-275664-qbafkxtr 239 55 biological biological JJ cord-275664-qbafkxtr 239 56 functions function NNS cord-275664-qbafkxtr 239 57 . . . cord-275664-qbafkxtr 240 1 Microbiol Microbiol NNP cord-275664-qbafkxtr 241 1 The the DT cord-275664-qbafkxtr 241 2 ERK ERK NNP cord-275664-qbafkxtr 241 3 cascade cascade NN cord-275664-qbafkxtr 241 4 : : : cord-275664-qbafkxtr 241 5 a a DT cord-275664-qbafkxtr 241 6 prototype prototype NN cord-275664-qbafkxtr 241 7 of of IN cord-275664-qbafkxtr 241 8 MAPK MAPK NNP cord-275664-qbafkxtr 241 9 signaling signal VBG cord-275664-qbafkxtr 241 10 Adenovirus Adenovirus NNP cord-275664-qbafkxtr 241 11 - - HYPH cord-275664-qbafkxtr 241 12 induced induce VBN cord-275664-qbafkxtr 241 13 extracellular extracellular JJ cord-275664-qbafkxtr 241 14 signalregulated signalregulate VBN cord-275664-qbafkxtr 241 15 kinase kinase NN cord-275664-qbafkxtr 241 16 phosphorylation phosphorylation NN cord-275664-qbafkxtr 241 17 during during IN cord-275664-qbafkxtr 241 18 the the DT cord-275664-qbafkxtr 241 19 late late JJ cord-275664-qbafkxtr 241 20 phase phase NN cord-275664-qbafkxtr 241 21 of of IN cord-275664-qbafkxtr 241 22 infection infection NN cord-275664-qbafkxtr 241 23 enhances enhance VBZ cord-275664-qbafkxtr 241 24 viral viral JJ cord-275664-qbafkxtr 241 25 protein protein NN cord-275664-qbafkxtr 241 26 levels level NNS cord-275664-qbafkxtr 241 27 and and CC cord-275664-qbafkxtr 241 28 virus virus NN cord-275664-qbafkxtr 241 29 progeny progeny NN cord-275664-qbafkxtr 241 30 Blockade blockade NN cord-275664-qbafkxtr 241 31 of of IN cord-275664-qbafkxtr 241 32 the the DT cord-275664-qbafkxtr 241 33 MAP map NN cord-275664-qbafkxtr 241 34 kinase kinase NN cord-275664-qbafkxtr 241 35 pathway pathway NN cord-275664-qbafkxtr 241 36 suppresses suppress VBZ cord-275664-qbafkxtr 241 37 growth growth NN cord-275664-qbafkxtr 241 38 of of IN cord-275664-qbafkxtr 241 39 colon colon NN cord-275664-qbafkxtr 241 40 tumors tumor NNS cord-275664-qbafkxtr 241 41 in in IN cord-275664-qbafkxtr 241 42 vivo vivo NNP cord-275664-qbafkxtr 241 43 Postweaning Postweaning NNP cord-275664-qbafkxtr 241 44 multisystemic multisystemic NNP cord-275664-qbafkxtr 241 45 wasting waste VBG cord-275664-qbafkxtr 241 46 syndrome syndrome NN cord-275664-qbafkxtr 241 47 ( ( -LRB- cord-275664-qbafkxtr 241 48 PMWS PMWS NNP cord-275664-qbafkxtr 241 49 ) ) -RRB- cord-275664-qbafkxtr 241 50 in in IN cord-275664-qbafkxtr 241 51 pigs pig NNS cord-275664-qbafkxtr 241 52 : : : cord-275664-qbafkxtr 241 53 a a DT cord-275664-qbafkxtr 241 54 review review NN cord-275664-qbafkxtr 241 55 Immunosuppression Immunosuppression NNP cord-275664-qbafkxtr 241 56 in in IN cord-275664-qbafkxtr 241 57 postweaning postweane VBG cord-275664-qbafkxtr 241 58 multisystemic multisystemic NNP cord-275664-qbafkxtr 241 59 wasting waste VBG cord-275664-qbafkxtr 241 60 syndrome syndrome NN cord-275664-qbafkxtr 241 61 affected affect VBD cord-275664-qbafkxtr 241 62 pigs pig NNS cord-275664-qbafkxtr 241 63 Demonstration Demonstration NNP cord-275664-qbafkxtr 241 64 of of IN cord-275664-qbafkxtr 241 65 nicking nick VBG cord-275664-qbafkxtr 241 66 / / SYM cord-275664-qbafkxtr 241 67 joining join VBG cord-275664-qbafkxtr 241 68 activity activity NN cord-275664-qbafkxtr 241 69 at at IN cord-275664-qbafkxtr 241 70 the the DT cord-275664-qbafkxtr 241 71 origin origin NN cord-275664-qbafkxtr 241 72 of of IN cord-275664-qbafkxtr 241 73 DNA dna NN cord-275664-qbafkxtr 241 74 replication replication NN cord-275664-qbafkxtr 241 75 associated associate VBN cord-275664-qbafkxtr 241 76 with with IN cord-275664-qbafkxtr 241 77 the the DT cord-275664-qbafkxtr 241 78 rep rep NNP cord-275664-qbafkxtr 241 79 and and CC cord-275664-qbafkxtr 241 80 rep rep NNP cord-275664-qbafkxtr 241 81 ' ' POS cord-275664-qbafkxtr 241 82 proteins protein NNS cord-275664-qbafkxtr 241 83 of of IN cord-275664-qbafkxtr 241 84 porcine porcine JJ cord-275664-qbafkxtr 241 85 circovirus circovirus JJ cord-275664-qbafkxtr 241 86 type type NN cord-275664-qbafkxtr 241 87 1 1 CD cord-275664-qbafkxtr 241 88 A a DT cord-275664-qbafkxtr 241 89 very very RB cord-275664-qbafkxtr 241 90 small small JJ cord-275664-qbafkxtr 241 91 porcine porcine JJ cord-275664-qbafkxtr 241 92 virus virus NN cord-275664-qbafkxtr 241 93 with with IN cord-275664-qbafkxtr 241 94 circular circular JJ cord-275664-qbafkxtr 241 95 single single RB cord-275664-qbafkxtr 241 96 - - HYPH cord-275664-qbafkxtr 241 97 stranded strand VBN cord-275664-qbafkxtr 241 98 DNA dna NN cord-275664-qbafkxtr 241 99 Replication replication NN cord-275664-qbafkxtr 241 100 of of IN cord-275664-qbafkxtr 241 101 porcine porcine JJ cord-275664-qbafkxtr 241 102 circovirus circovirus NN cord-275664-qbafkxtr 241 103 : : : cord-275664-qbafkxtr 241 104 induction induction NN cord-275664-qbafkxtr 241 105 by by IN cord-275664-qbafkxtr 241 106 glucosamine glucosamine NN cord-275664-qbafkxtr 241 107 and and CC cord-275664-qbafkxtr 241 108 cell cell NN cord-275664-qbafkxtr 241 109 cycle cycle NN cord-275664-qbafkxtr 241 110 dependence dependence NN cord-275664-qbafkxtr 242 1 Circoviridae Circoviridae NNP cord-275664-qbafkxtr 242 2 Porcine porcine JJ cord-275664-qbafkxtr 242 3 circovirus circovirus JJ cord-275664-qbafkxtr 242 4 type type NN cord-275664-qbafkxtr 242 5 2 2 CD cord-275664-qbafkxtr 242 6 induces induce VBZ cord-275664-qbafkxtr 242 7 the the DT cord-275664-qbafkxtr 242 8 activation activation NN cord-275664-qbafkxtr 242 9 of of IN cord-275664-qbafkxtr 242 10 nuclear nuclear JJ cord-275664-qbafkxtr 242 11 factor factor NN cord-275664-qbafkxtr 242 12 kappa kappa NN cord-275664-qbafkxtr 242 13 B B NNP cord-275664-qbafkxtr 242 14 by by IN cord-275664-qbafkxtr 242 15 IκBα IκBα NNP cord-275664-qbafkxtr 242 16 degradation degradation NN cord-275664-qbafkxtr 242 17 Phosphorylation Phosphorylation NNP cord-275664-qbafkxtr 242 18 within within IN cord-275664-qbafkxtr 242 19 the the DT cord-275664-qbafkxtr 242 20 transactivation transactivation NN cord-275664-qbafkxtr 242 21 domain domain NN cord-275664-qbafkxtr 242 22 of of IN cord-275664-qbafkxtr 242 23 adenovirus adenovirus NN cord-275664-qbafkxtr 242 24 E1A e1a NN cord-275664-qbafkxtr 242 25 protein protein NN cord-275664-qbafkxtr 242 26 by by IN cord-275664-qbafkxtr 242 27 mitogen mitogen NN cord-275664-qbafkxtr 242 28 - - HYPH cord-275664-qbafkxtr 242 29 activated activate VBN cord-275664-qbafkxtr 242 30 protein protein NN cord-275664-qbafkxtr 242 31 kinase kinase NN cord-275664-qbafkxtr 242 32 regulates regulate VBZ cord-275664-qbafkxtr 242 33 expression expression NN cord-275664-qbafkxtr 242 34 of of IN cord-275664-qbafkxtr 242 35 early early JJ cord-275664-qbafkxtr 242 36 region region NN cord-275664-qbafkxtr 242 37 4 4 CD cord-275664-qbafkxtr 242 38 Mitogen Mitogen NNP cord-275664-qbafkxtr 242 39 - - HYPH cord-275664-qbafkxtr 242 40 activated activate VBN cord-275664-qbafkxtr 242 41 protein protein NN cord-275664-qbafkxtr 242 42 kinase kinase NN cord-275664-qbafkxtr 242 43 phosphorylates phosphorylate VBZ cord-275664-qbafkxtr 242 44 and and CC cord-275664-qbafkxtr 242 45 regulates regulate VBZ cord-275664-qbafkxtr 242 46 the the DT cord-275664-qbafkxtr 242 47 HIV-1 HIV-1 NNP cord-275664-qbafkxtr 242 48 Vif Vif NNP cord-275664-qbafkxtr 242 49 protein protein NN cord-275664-qbafkxtr 242 50 Regulation regulation NN cord-275664-qbafkxtr 242 51 of of IN cord-275664-qbafkxtr 242 52 human human JJ cord-275664-qbafkxtr 242 53 immunodeficiency immunodeficiency NN cord-275664-qbafkxtr 242 54 virus virus NN cord-275664-qbafkxtr 242 55 type type NN cord-275664-qbafkxtr 242 56 1 1 CD cord-275664-qbafkxtr 242 57 infectivity infectivity NN cord-275664-qbafkxtr 242 58 by by IN cord-275664-qbafkxtr 242 59 the the DT cord-275664-qbafkxtr 242 60 ERK ERK NNP cord-275664-qbafkxtr 242 61 mitogen mitogen NNP cord-275664-qbafkxtr 242 62 - - HYPH cord-275664-qbafkxtr 242 63 activated activate VBN cord-275664-qbafkxtr 242 64 protein protein NN cord-275664-qbafkxtr 242 65 kinase kinase NN cord-275664-qbafkxtr 242 66 signaling signal VBG cord-275664-qbafkxtr 242 67 pathway pathway NN cord-275664-qbafkxtr 242 68 This this DT cord-275664-qbafkxtr 242 69 work work NN cord-275664-qbafkxtr 242 70 was be VBD cord-275664-qbafkxtr 242 71 supported support VBN cord-275664-qbafkxtr 242 72 by by IN cord-275664-qbafkxtr 242 73 grants grant NNS cord-275664-qbafkxtr 242 74 from from IN cord-275664-qbafkxtr 242 75 National National NNP cord-275664-qbafkxtr 242 76 Natural Natural NNP cord-275664-qbafkxtr 242 77 Science Science NNP cord-275664-qbafkxtr 242 78 Foundation Foundation NNP cord-275664-qbafkxtr 242 79 ( ( -LRB- cord-275664-qbafkxtr 242 80 30871866 30871866 CD cord-275664-qbafkxtr 242 81 ) ) -RRB- cord-275664-qbafkxtr 242 82 and and CC cord-275664-qbafkxtr 242 83 Beijing Beijing NNP cord-275664-qbafkxtr 242 84 Municipal Municipal NNP cord-275664-qbafkxtr 242 85 Science Science NNP cord-275664-qbafkxtr 242 86 and and CC cord-275664-qbafkxtr 242 87 Technology Technology NNP cord-275664-qbafkxtr 242 88 Contract Contract NNP cord-275664-qbafkxtr 242 89 Project Project NNP cord-275664-qbafkxtr 242 90 ( ( -LRB- cord-275664-qbafkxtr 242 91 Z07010501780701 Z07010501780701 NNP cord-275664-qbafkxtr 242 92 ) ) -RRB- cord-275664-qbafkxtr 242 93 , , , cord-275664-qbafkxtr 242 94 the the DT cord-275664-qbafkxtr 242 95 People People NNPS cord-275664-qbafkxtr 242 96 's 's POS cord-275664-qbafkxtr 242 97 Republic Republic NNP cord-275664-qbafkxtr 242 98 of of IN cord-275664-qbafkxtr 242 99 China China NNP cord-275664-qbafkxtr 242 100 . . .