id sid tid token lemma pos cord-283998-whwksoxt 1 1 key key NN cord-283998-whwksoxt 1 2 : : : cord-283998-whwksoxt 2 1 cord-283998-whwksoxt cord-283998-whwksoxt NNP cord-283998-whwksoxt 3 1 authors author NNS cord-283998-whwksoxt 3 2 : : : cord-283998-whwksoxt 3 3 Tannock Tannock NNP cord-283998-whwksoxt 3 4 , , , cord-283998-whwksoxt 3 5 Gregory Gregory NNP cord-283998-whwksoxt 3 6 A. A. NNP cord-283998-whwksoxt 3 7 ; ; : cord-283998-whwksoxt 3 8 Hierholzer Hierholzer NNP cord-283998-whwksoxt 3 9 , , , cord-283998-whwksoxt 3 10 John John NNP cord-283998-whwksoxt 3 11 C. C. NNP cord-283998-whwksoxt 3 12 title title NN cord-283998-whwksoxt 3 13 : : : cord-283998-whwksoxt 4 1 The the DT cord-283998-whwksoxt 4 2 RNA RNA NNP cord-283998-whwksoxt 4 3 of of IN cord-283998-whwksoxt 4 4 human human JJ cord-283998-whwksoxt 4 5 coronavirus coronavirus NN cord-283998-whwksoxt 4 6 OC-43 OC-43 NNP cord-283998-whwksoxt 4 7 date date NN cord-283998-whwksoxt 4 8 : : : cord-283998-whwksoxt 4 9 1977 1977 CD cord-283998-whwksoxt 4 10 - - SYM cord-283998-whwksoxt 4 11 12 12 CD cord-283998-whwksoxt 4 12 - - SYM cord-283998-whwksoxt 4 13 31 31 CD cord-283998-whwksoxt 4 14 journal journal NN cord-283998-whwksoxt 4 15 : : : cord-283998-whwksoxt 5 1 Virology virology NN cord-283998-whwksoxt 5 2 DOI DOI NNP cord-283998-whwksoxt 5 3 : : : cord-283998-whwksoxt 5 4 10.1016/0042 10.1016/0042 CD cord-283998-whwksoxt 5 5 - - SYM cord-283998-whwksoxt 5 6 6822(77)90126-x 6822(77)90126-x CD cord-283998-whwksoxt 5 7 sha sha NNP cord-283998-whwksoxt 5 8 : : : cord-283998-whwksoxt 6 1 b6e753f1d598feaca24c0a19464fe7e2c503cc31 b6e753f1d598feaca24c0a19464fe7e2c503cc31 NNP cord-283998-whwksoxt 6 2 doc_id doc_id CD cord-283998-whwksoxt 6 3 : : : cord-283998-whwksoxt 6 4 283998 283998 CD cord-283998-whwksoxt 6 5 cord_uid cord_uid NNS cord-283998-whwksoxt 6 6 : : : cord-283998-whwksoxt 7 1 whwksoxt whwksoxt NNP cord-283998-whwksoxt 7 2 Abstract Abstract NNP cord-283998-whwksoxt 7 3 A a DT cord-283998-whwksoxt 7 4 homogeneous homogeneous JJ cord-283998-whwksoxt 7 5 RNA RNA NNP cord-283998-whwksoxt 7 6 complex complex NN cord-283998-whwksoxt 7 7 with with IN cord-283998-whwksoxt 7 8 a a DT cord-283998-whwksoxt 7 9 sedimentation sedimentation NN cord-283998-whwksoxt 7 10 coefficient coefficient NN cord-283998-whwksoxt 7 11 of of IN cord-283998-whwksoxt 7 12 70 70 CD cord-283998-whwksoxt 7 13 S S NNS cord-283998-whwksoxt 7 14 and and CC cord-283998-whwksoxt 7 15 an an DT cord-283998-whwksoxt 7 16 apparent apparent JJ cord-283998-whwksoxt 7 17 molecular molecular JJ cord-283998-whwksoxt 7 18 weight weight NN cord-283998-whwksoxt 7 19 of of IN cord-283998-whwksoxt 7 20 approximately approximately RB cord-283998-whwksoxt 7 21 6.1 6.1 CD cord-283998-whwksoxt 7 22 × × NN cord-283998-whwksoxt 7 23 106 106 CD cord-283998-whwksoxt 7 24 was be VBD cord-283998-whwksoxt 7 25 released release VBN cord-283998-whwksoxt 7 26 from from IN cord-283998-whwksoxt 7 27 purified purify VBN cord-283998-whwksoxt 7 28 32P 32p CD cord-283998-whwksoxt 7 29 - - HYPH cord-283998-whwksoxt 7 30 labeled label VBN cord-283998-whwksoxt 7 31 , , , cord-283998-whwksoxt 7 32 mouse mouse NN cord-283998-whwksoxt 7 33 - - HYPH cord-283998-whwksoxt 7 34 brain brain NN cord-283998-whwksoxt 7 35 - - HYPH cord-283998-whwksoxt 7 36 derived derive VBN cord-283998-whwksoxt 7 37 OC-43 OC-43 NNP cord-283998-whwksoxt 7 38 virus virus NN cord-283998-whwksoxt 7 39 after after IN cord-283998-whwksoxt 7 40 treatment treatment NN cord-283998-whwksoxt 7 41 with with IN cord-283998-whwksoxt 7 42 1 1 CD cord-283998-whwksoxt 7 43 % % NN cord-283998-whwksoxt 7 44 sodium sodium NN cord-283998-whwksoxt 7 45 dodecyl dodecyl NNP cord-283998-whwksoxt 7 46 sulfate sulfate NNP cord-283998-whwksoxt 7 47 ( ( -LRB- cord-283998-whwksoxt 7 48 SDS SDS NNP cord-283998-whwksoxt 7 49 ) ) -RRB- cord-283998-whwksoxt 7 50 for for IN cord-283998-whwksoxt 7 51 15 15 CD cord-283998-whwksoxt 7 52 min min NN cord-283998-whwksoxt 7 53 at at IN cord-283998-whwksoxt 7 54 23 23 CD cord-283998-whwksoxt 7 55 ° ° NNS cord-283998-whwksoxt 7 56 . . . cord-283998-whwksoxt 8 1 The the DT cord-283998-whwksoxt 8 2 complex complex NN cord-283998-whwksoxt 8 3 was be VBD cord-283998-whwksoxt 8 4 highly highly RB cord-283998-whwksoxt 8 5 susceptible susceptible JJ cord-283998-whwksoxt 8 6 to to IN cord-283998-whwksoxt 8 7 heat heat NN cord-283998-whwksoxt 8 8 , , , cord-283998-whwksoxt 8 9 releasing release VBG cord-283998-whwksoxt 8 10 4 4 CD cord-283998-whwksoxt 8 11 S S NNP cord-283998-whwksoxt 8 12 RNA RNA NNP cord-283998-whwksoxt 8 13 fragments fragment NNS cord-283998-whwksoxt 8 14 at at IN cord-283998-whwksoxt 8 15 37 37 CD cord-283998-whwksoxt 8 16 ° ° NNS cord-283998-whwksoxt 8 17 and and CC cord-283998-whwksoxt 8 18 breaking break VBG cord-283998-whwksoxt 8 19 down down RP cord-283998-whwksoxt 8 20 to to IN cord-283998-whwksoxt 8 21 fragments fragment NNS cord-283998-whwksoxt 8 22 of of IN cord-283998-whwksoxt 8 23 4–70 4–70 CD cord-283998-whwksoxt 8 24 S S NNP cord-283998-whwksoxt 8 25 at at IN cord-283998-whwksoxt 8 26 60 60 CD cord-283998-whwksoxt 8 27 ° ° NNS cord-283998-whwksoxt 8 28 ; ; : cord-283998-whwksoxt 8 29 it -PRON- PRP cord-283998-whwksoxt 8 30 was be VBD cord-283998-whwksoxt 8 31 also also RB cord-283998-whwksoxt 8 32 degraded degrade VBN cord-283998-whwksoxt 8 33 by by IN cord-283998-whwksoxt 8 34 centrifugation centrifugation NN cord-283998-whwksoxt 8 35 through through IN cord-283998-whwksoxt 8 36 dimethyl dimethyl NN cord-283998-whwksoxt 8 37 sulfoxide sulfoxide NN cord-283998-whwksoxt 8 38 gradients gradient NNS cord-283998-whwksoxt 8 39 . . . cord-283998-whwksoxt 9 1 Unlike unlike IN cord-283998-whwksoxt 9 2 tobacco tobacco NN cord-283998-whwksoxt 9 3 mosaic mosaic NN cord-283998-whwksoxt 9 4 virus virus NN cord-283998-whwksoxt 9 5 or or CC cord-283998-whwksoxt 9 6 Rous rous JJ cord-283998-whwksoxt 9 7 sarcoma sarcoma NN cord-283998-whwksoxt 9 8 virus virus NN cord-283998-whwksoxt 9 9 RNA RNA NNP cord-283998-whwksoxt 9 10 , , , cord-283998-whwksoxt 9 11 OC-43 OC-43 NNP cord-283998-whwksoxt 9 12 RNA RNA NNP cord-283998-whwksoxt 9 13 prepared prepare VBN cord-283998-whwksoxt 9 14 by by IN cord-283998-whwksoxt 9 15 extraction extraction NN cord-283998-whwksoxt 9 16 with with IN cord-283998-whwksoxt 9 17 phenol phenol NN cord-283998-whwksoxt 9 18 - - HYPH cord-283998-whwksoxt 9 19 SDS sds NN cord-283998-whwksoxt 9 20 or or CC cord-283998-whwksoxt 9 21 phenol phenol NN cord-283998-whwksoxt 9 22 - - HYPH cord-283998-whwksoxt 9 23 chloroform chloroform NN cord-283998-whwksoxt 9 24 degraded degrade VBN cord-283998-whwksoxt 9 25 into into IN cord-283998-whwksoxt 9 26 a a DT cord-283998-whwksoxt 9 27 range range NN cord-283998-whwksoxt 9 28 of of IN cord-283998-whwksoxt 9 29 fragments fragment NNS cord-283998-whwksoxt 9 30 with with IN cord-283998-whwksoxt 9 31 coefficients coefficient NNS cord-283998-whwksoxt 9 32 of of IN cord-283998-whwksoxt 9 33 15–55 15–55 JJ cord-283998-whwksoxt 9 34 S S NNP cord-283998-whwksoxt 9 35 ; ; : cord-283998-whwksoxt 9 36 4 4 CD cord-283998-whwksoxt 9 37 S S NNP cord-283998-whwksoxt 9 38 RNA RNA NNP cord-283998-whwksoxt 9 39 was be VBD cord-283998-whwksoxt 9 40 also also RB cord-283998-whwksoxt 9 41 present present JJ cord-283998-whwksoxt 9 42 as as IN cord-283998-whwksoxt 9 43 a a DT cord-283998-whwksoxt 9 44 minor minor JJ cord-283998-whwksoxt 9 45 component component NN cord-283998-whwksoxt 9 46 . . . cord-283998-whwksoxt 10 1 This this DT cord-283998-whwksoxt 10 2 suggests suggest VBZ cord-283998-whwksoxt 10 3 that that IN cord-283998-whwksoxt 10 4 ( ( -LRB- cord-283998-whwksoxt 10 5 a a DT cord-283998-whwksoxt 10 6 ) ) -RRB- cord-283998-whwksoxt 10 7 extensive extensive JJ cord-283998-whwksoxt 10 8 nicking nicking NN cord-283998-whwksoxt 10 9 of of IN cord-283998-whwksoxt 10 10 a a DT cord-283998-whwksoxt 10 11 large large JJ cord-283998-whwksoxt 10 12 RNA RNA NNP cord-283998-whwksoxt 10 13 molecule molecule NN cord-283998-whwksoxt 10 14 has have VBZ cord-283998-whwksoxt 10 15 occurred occur VBN cord-283998-whwksoxt 10 16 during during IN cord-283998-whwksoxt 10 17 viral viral JJ cord-283998-whwksoxt 10 18 growth growth NN cord-283998-whwksoxt 10 19 , , , cord-283998-whwksoxt 10 20 due due IN cord-283998-whwksoxt 10 21 to to IN cord-283998-whwksoxt 10 22 ribonucleases ribonuclease NNS cord-283998-whwksoxt 10 23 which which WDT cord-283998-whwksoxt 10 24 are be VBP cord-283998-whwksoxt 10 25 inactivated inactivate VBN cord-283998-whwksoxt 10 26 during during IN cord-283998-whwksoxt 10 27 phenol phenol NN cord-283998-whwksoxt 10 28 extractions extraction NNS cord-283998-whwksoxt 10 29 ; ; , cord-283998-whwksoxt 10 30 ( ( -LRB- cord-283998-whwksoxt 10 31 b b NN cord-283998-whwksoxt 10 32 ) ) -RRB- cord-283998-whwksoxt 10 33 heterogeneity heterogeneity NN cord-283998-whwksoxt 10 34 for for IN cord-283998-whwksoxt 10 35 OC-43 OC-43 NNP cord-283998-whwksoxt 10 36 RNA RNA NNP cord-283998-whwksoxt 10 37 is be VBZ cord-283998-whwksoxt 10 38 not not RB cord-283998-whwksoxt 10 39 due due JJ cord-283998-whwksoxt 10 40 to to IN cord-283998-whwksoxt 10 41 internal internal JJ cord-283998-whwksoxt 10 42 ribonuclease ribonuclease NN cord-283998-whwksoxt 10 43 activity activity NN cord-283998-whwksoxt 10 44 and and CC cord-283998-whwksoxt 10 45 fragments fragment NNS cord-283998-whwksoxt 10 46 are be VBP cord-283998-whwksoxt 10 47 held hold VBN cord-283998-whwksoxt 10 48 together together RB cord-283998-whwksoxt 10 49 by by IN cord-283998-whwksoxt 10 50 noncovalent noncovalent NN cord-283998-whwksoxt 10 51 linkages linkage NNS cord-283998-whwksoxt 10 52 much much RB cord-283998-whwksoxt 10 53 weaker weak JJR cord-283998-whwksoxt 10 54 than than IN cord-283998-whwksoxt 10 55 those those DT cord-283998-whwksoxt 10 56 present present JJ cord-283998-whwksoxt 10 57 in in IN cord-283998-whwksoxt 10 58 the the DT cord-283998-whwksoxt 10 59 70 70 CD cord-283998-whwksoxt 10 60 S S NNP cord-283998-whwksoxt 10 61 retroviral retroviral NN cord-283998-whwksoxt 10 62 RNA rna NN cord-283998-whwksoxt 10 63 complex complex NN cord-283998-whwksoxt 10 64 , , , cord-283998-whwksoxt 10 65 or or CC cord-283998-whwksoxt 10 66 by by IN cord-283998-whwksoxt 10 67 small small JJ cord-283998-whwksoxt 10 68 proteins protein NNS cord-283998-whwksoxt 10 69 ; ; : cord-283998-whwksoxt 10 70 or or CC cord-283998-whwksoxt 10 71 , , , cord-283998-whwksoxt 10 72 most most RBS cord-283998-whwksoxt 10 73 probably probably RB cord-283998-whwksoxt 10 74 , , , cord-283998-whwksoxt 10 75 ( ( -LRB- cord-283998-whwksoxt 10 76 c c NN cord-283998-whwksoxt 10 77 ) ) -RRB- cord-283998-whwksoxt 10 78 a a DT cord-283998-whwksoxt 10 79 combination combination NN cord-283998-whwksoxt 10 80 of of IN cord-283998-whwksoxt 10 81 extensive extensive JJ cord-283998-whwksoxt 10 82 nicking nicking NN cord-283998-whwksoxt 10 83 and and CC cord-283998-whwksoxt 10 84 weak weak JJ cord-283998-whwksoxt 10 85 noncovalent noncovalent NN cord-283998-whwksoxt 10 86 linkages linkage NNS cord-283998-whwksoxt 10 87 results result VBZ cord-283998-whwksoxt 10 88 in in IN cord-283998-whwksoxt 10 89 the the DT cord-283998-whwksoxt 10 90 heterogeneous heterogeneous JJ cord-283998-whwksoxt 10 91 denaturation denaturation NN cord-283998-whwksoxt 10 92 products product NNS cord-283998-whwksoxt 10 93 . . . cord-283998-whwksoxt 11 1 or or CC cord-283998-whwksoxt 11 2 , , , cord-283998-whwksoxt 11 3 most most RBS cord-283998-whwksoxt 11 4 probably probably RB cord-283998-whwksoxt 11 5 , , , cord-283998-whwksoxt 11 6 ( ( -LRB- cord-283998-whwksoxt 11 7 cl cl NNP cord-283998-whwksoxt 11 8 a a DT cord-283998-whwksoxt 11 9 combination combination NN cord-283998-whwksoxt 11 10 of of IN cord-283998-whwksoxt 11 11 extensive extensive JJ cord-283998-whwksoxt 11 12 nicking nicking NN cord-283998-whwksoxt 11 13 and and CC cord-283998-whwksoxt 11 14 weak weak JJ cord-283998-whwksoxt 11 15 noncovalent noncovalent NN cord-283998-whwksoxt 11 16 linkages linkage NNS cord-283998-whwksoxt 12 1 results result NNS cord-283998-whwksoxt 12 2 in in IN cord-283998-whwksoxt 12 3 the the DT cord-283998-whwksoxt 12 4 heterogeneous heterogeneous JJ cord-283998-whwksoxt 12 5 denaturation denaturation NN cord-283998-whwksoxt 12 6 products product NNS cord-283998-whwksoxt 12 7 . . . cord-283998-whwksoxt 13 1 The the DT cord-283998-whwksoxt 13 2 Coronaviridae Coronaviridae NNP cord-283998-whwksoxt 13 3 were be VBD cord-283998-whwksoxt 13 4 described describe VBN cord-283998-whwksoxt 13 5 as as IN cord-283998-whwksoxt 13 6 a a DT cord-283998-whwksoxt 13 7 separate separate JJ cord-283998-whwksoxt 13 8 taxonomic taxonomic JJ cord-283998-whwksoxt 13 9 group group NN cord-283998-whwksoxt 13 10 by by IN cord-283998-whwksoxt 13 11 Almeida Almeida NNP cord-283998-whwksoxt 13 12 et et NNP cord-283998-whwksoxt 13 13 al al NNP cord-283998-whwksoxt 13 14 . . . cord-283998-whwksoxt 14 1 ( ( -LRB- cord-283998-whwksoxt 14 2 1968 1968 CD cord-283998-whwksoxt 14 3 ) ) -RRB- cord-283998-whwksoxt 15 1 but but CC cord-283998-whwksoxt 15 2 , , , cord-283998-whwksoxt 15 3 despite despite IN cord-283998-whwksoxt 15 4 their -PRON- PRP$ cord-283998-whwksoxt 15 5 ubiquity ubiquity NN cord-283998-whwksoxt 15 6 and and CC cord-283998-whwksoxt 15 7 economic economic JJ cord-283998-whwksoxt 15 8 importance importance NN cord-283998-whwksoxt 15 9 , , , cord-283998-whwksoxt 15 10 few few JJ cord-283998-whwksoxt 15 11 biophysical biophysical JJ cord-283998-whwksoxt 15 12 characterization characterization NN cord-283998-whwksoxt 15 13 studies study NNS cord-283998-whwksoxt 15 14 have have VBP cord-283998-whwksoxt 15 15 been be VBN cord-283998-whwksoxt 15 16 reported report VBN cord-283998-whwksoxt 15 17 . . . cord-283998-whwksoxt 16 1 The the DT cord-283998-whwksoxt 16 2 RNA RNA NNP cord-283998-whwksoxt 16 3 of of IN cord-283998-whwksoxt 16 4 avian avian JJ cord-283998-whwksoxt 16 5 infectious infectious JJ cord-283998-whwksoxt 16 6 bronchitis bronchitis NN cord-283998-whwksoxt 16 7 virus virus NN cord-283998-whwksoxt 16 8 ( ( -LRB- cord-283998-whwksoxt 16 9 IBV IBV NNP cord-283998-whwksoxt 16 10 ) ) -RRB- cord-283998-whwksoxt 16 11 was be VBD cord-283998-whwksoxt 16 12 first first RB cord-283998-whwksoxt 16 13 described describe VBN cord-283998-whwksoxt 16 14 by by IN cord-283998-whwksoxt 16 15 Tannock Tannock NNP cord-283998-whwksoxt 16 16 ( ( -LRB- cord-283998-whwksoxt 16 17 1973 1973 CD cord-283998-whwksoxt 16 18 ) ) -RRB- cord-283998-whwksoxt 16 19 , , , cord-283998-whwksoxt 16 20 who who WP cord-283998-whwksoxt 16 21 obtained obtain VBD cord-283998-whwksoxt 16 22 from from IN cord-283998-whwksoxt 16 23 purified purify VBN cord-283998-whwksoxt 16 24 virions virion NNS cord-283998-whwksoxt 16 25 a a DT cord-283998-whwksoxt 16 26 highly highly RB cord-283998-whwksoxt 16 27 heterogeneous heterogeneous JJ cord-283998-whwksoxt 16 28 array array NN cord-283998-whwksoxt 16 29 of of IN cord-283998-whwksoxt 16 30 RNA rna NN cord-283998-whwksoxt 16 31 fragments fragment NNS cord-283998-whwksoxt 16 32 using use VBG cord-283998-whwksoxt 16 33 a a DT cord-283998-whwksoxt 16 34 phenol phenol NN cord-283998-whwksoxt 16 35 - - HYPH cord-283998-whwksoxt 16 36 sodium sodium NN cord-283998-whwksoxt 16 37 dodecyl dodecyl NNP cord-283998-whwksoxt 16 38 sulfate sulfate NNP cord-283998-whwksoxt 16 39 ( ( -LRB- cord-283998-whwksoxt 16 40 SDS SDS NNP cord-283998-whwksoxt 16 41 ) ) -RRB- cord-283998-whwksoxt 16 42 extraction extraction NN cord-283998-whwksoxt 16 43 procedure procedure NN cord-283998-whwksoxt 16 44 . . . cord-283998-whwksoxt 17 1 Fragments fragment NNS cord-283998-whwksoxt 17 2 ranged range VBD cord-283998-whwksoxt 17 3 in in IN cord-283998-whwksoxt 17 4 molecular molecular JJ cord-283998-whwksoxt 17 5 weight weight NN cord-283998-whwksoxt 17 6 from from IN cord-283998-whwksoxt 17 7 0.5 0.5 CD cord-283998-whwksoxt 17 8 x x SYM cord-283998-whwksoxt 17 9 lo6 lo6 NNS cord-283998-whwksoxt 17 10 to to IN cord-283998-whwksoxt 17 11 more more JJR cord-283998-whwksoxt 17 12 than than IN cord-283998-whwksoxt 17 13 3.0 3.0 CD cord-283998-whwksoxt 17 14 x x SYM cord-283998-whwksoxt 17 15 106 106 CD cord-283998-whwksoxt 17 16 ; ; : cord-283998-whwksoxt 17 17 a a DT cord-283998-whwksoxt 17 18 smaller small JJR cord-283998-whwksoxt 17 19 , , , cord-283998-whwksoxt 17 20 more more RBR cord-283998-whwksoxt 17 21 homogeneous homogeneous JJ cord-283998-whwksoxt 17 22 class class NN cord-283998-whwksoxt 17 23 of of IN cord-283998-whwksoxt 17 24 a a DT cord-283998-whwksoxt 17 25 size size NN cord-283998-whwksoxt 17 26 similar similar JJ cord-283998-whwksoxt 17 27 to to IN cord-283998-whwksoxt 17 28 ribosomal ribosomal JJ cord-283998-whwksoxt 17 29 4 4 CD cord-283998-whwksoxt 17 30 S S NNP cord-283998-whwksoxt 17 31 RNA RNA NNP cord-283998-whwksoxt 17 32 was be VBD cord-283998-whwksoxt 17 33 also also RB cord-283998-whwksoxt 17 34 present present JJ cord-283998-whwksoxt 17 35 . . . cord-283998-whwksoxt 18 1 More more RBR cord-283998-whwksoxt 18 2 recently recently RB cord-283998-whwksoxt 18 3 , , , cord-283998-whwksoxt 18 4 Watkins Watkins NNP cord-283998-whwksoxt 18 5 et et NNP cord-283998-whwksoxt 18 6 al al NNP cord-283998-whwksoxt 18 7 . . . cord-283998-whwksoxt 19 1 ( ( -LRB- cord-283998-whwksoxt 19 2 1975 1975 CD cord-283998-whwksoxt 19 3 ) ) -RRB- cord-283998-whwksoxt 19 4 scribed scribe VBD cord-283998-whwksoxt 19 5 IBV IBV NNP cord-283998-whwksoxt 19 6 RNA RNA NNP cord-283998-whwksoxt 19 7 , , , cord-283998-whwksoxt 19 8 after after IN cord-283998-whwksoxt 19 9 its -PRON- PRP$ cord-283998-whwksoxt 19 10 release release NN cord-283998-whwksoxt 19 11 from from IN cord-283998-whwksoxt 19 12 virions virion NNS cord-283998-whwksoxt 19 13 by by IN cord-283998-whwksoxt 19 14 SDS sds NN cord-283998-whwksoxt 19 15 lysis lysis NN cord-283998-whwksoxt 19 16 , , , cord-283998-whwksoxt 19 17 as as IN cord-283998-whwksoxt 19 18 a a DT cord-283998-whwksoxt 19 19 single single JJ cord-283998-whwksoxt 19 20 species specie NNS cord-283998-whwksoxt 19 21 having have VBG cord-283998-whwksoxt 19 22 a a DT cord-283998-whwksoxt 19 23 molecular molecular JJ cord-283998-whwksoxt 19 24 weight weight NN cord-283998-whwksoxt 19 25 of of IN cord-283998-whwksoxt 19 26 9.0 9.0 CD cord-283998-whwksoxt 19 27 x x SYM cord-283998-whwksoxt 19 28 lo6 lo6 NNS cord-283998-whwksoxt 19 29 and and CC cord-283998-whwksoxt 19 30 a a DT cord-283998-whwksoxt 19 31 sedimentation sedimentation NN cord-283998-whwksoxt 19 32 coefficient coefficient NN cord-283998-whwksoxt 19 33 of of IN cord-283998-whwksoxt 19 34 50 50 CD cord-283998-whwksoxt 19 35 S. S. NNP cord-283998-whwksoxt 19 36 They -PRON- PRP cord-283998-whwksoxt 19 37 reported report VBD cord-283998-whwksoxt 19 38 , , , cord-283998-whwksoxt 19 39 but but CC cord-283998-whwksoxt 19 40 without without IN cord-283998-whwksoxt 19 41 supporting support VBG cord-283998-whwksoxt 19 42 evidence evidence NN cord-283998-whwksoxt 19 43 , , , cord-283998-whwksoxt 19 44 that that IN cord-283998-whwksoxt 19 45 by by IN cord-283998-whwksoxt 19 46 phenol phenol NN cord-283998-whwksoxt 19 47 extraction extraction NN cord-283998-whwksoxt 19 48 a a DT cord-283998-whwksoxt 19 49 range range NN cord-283998-whwksoxt 19 50 of of IN cord-283998-whwksoxt 19 51 RNA rna NN cord-283998-whwksoxt 19 52 fragments fragment NNS cord-283998-whwksoxt 19 53 could could MD cord-283998-whwksoxt 19 54 be be VB cord-283998-whwksoxt 19 55 obtained obtain VBN cord-283998-whwksoxt 19 56 which which WDT cord-283998-whwksoxt 19 57 was be VBD cord-283998-whwksoxt 19 58 similar similar JJ cord-283998-whwksoxt 19 59 to to IN cord-283998-whwksoxt 19 60 that that DT cord-283998-whwksoxt 20 1 Tannock Tannock NNP cord-283998-whwksoxt 20 2 ( ( -LRB- cord-283998-whwksoxt 20 3 1973 1973 CD cord-283998-whwksoxt 20 4 ) ) -RRB- cord-283998-whwksoxt 20 5 described describe VBD cord-283998-whwksoxt 20 6 . . . cord-283998-whwksoxt 21 1 Garwes Garwes NNP cord-283998-whwksoxt 21 2 et et FW cord-283998-whwksoxt 21 3 al al NNP cord-283998-whwksoxt 21 4 . . . cord-283998-whwksoxt 22 1 ( ( -LRB- cord-283998-whwksoxt 22 2 1975 1975 CD cord-283998-whwksoxt 22 3 ) ) -RRB- cord-283998-whwksoxt 22 4 examined examine VBD cord-283998-whwksoxt 22 5 the the DT cord-283998-whwksoxt 22 6 RNAs rna NNS cord-283998-whwksoxt 22 7 of of IN cord-283998-whwksoxt 22 8 two two CD cord-283998-whwksoxt 22 9 porcine porcine JJ cord-283998-whwksoxt 22 10 coronaviruses coronaviruse NNS cord-283998-whwksoxt 22 11 , , , cord-283998-whwksoxt 22 12 transmissible transmissible JJ cord-283998-whwksoxt 22 13 gastroenteritis gastroenteritis NN cord-283998-whwksoxt 22 14 virus virus NN cord-283998-whwksoxt 22 15 ( ( -LRB- cord-283998-whwksoxt 22 16 TGEV TGEV NNP cord-283998-whwksoxt 22 17 ) ) -RRB- cord-283998-whwksoxt 22 18 and and CC cord-283998-whwksoxt 22 19 hemagglutinating hemagglutinate VBG cord-283998-whwksoxt 22 20 encephalomyelitis encephalomyelitis NN cord-283998-whwksoxt 22 21 virus virus NN cord-283998-whwksoxt 22 22 ( ( -LRB- cord-283998-whwksoxt 22 23 HEV HEV NNP cord-283998-whwksoxt 22 24 ) ) -RRB- cord-283998-whwksoxt 22 25 , , , cord-283998-whwksoxt 22 26 after after IN cord-283998-whwksoxt 22 27 preparation preparation NN cord-283998-whwksoxt 22 28 by by IN cord-283998-whwksoxt 22 29 SDS sds NN cord-283998-whwksoxt 22 30 lysis lysis NN cord-283998-whwksoxt 22 31 . . . cord-283998-whwksoxt 23 1 They -PRON- PRP cord-283998-whwksoxt 23 2 obtained obtain VBD cord-283998-whwksoxt 23 3 a a DT cord-283998-whwksoxt 23 4 single single JJ cord-283998-whwksoxt 23 5 large large JJ cord-283998-whwksoxt 23 6 RNA RNA NNP cord-283998-whwksoxt 23 7 peak peak NN cord-283998-whwksoxt 23 8 with with IN cord-283998-whwksoxt 23 9 electrophoretic electrophoretic JJ cord-283998-whwksoxt 23 10 mobility mobility NN cord-283998-whwksoxt 23 11 similar similar JJ cord-283998-whwksoxt 23 12 to to IN cord-283998-whwksoxt 23 13 that that DT cord-283998-whwksoxt 23 14 of of IN cord-283998-whwksoxt 23 15 70 70 CD cord-283998-whwksoxt 23 16 S S NNP cord-283998-whwksoxt 23 17 Rous rous JJ cord-283998-whwksoxt 23 18 sarcoma sarcoma NN cord-283998-whwksoxt 23 19 virus virus NN cord-283998-whwksoxt 23 20 ( ( -LRB- cord-283998-whwksoxt 23 21 RSV RSV NNP cord-283998-whwksoxt 23 22 ) ) -RRB- cord-283998-whwksoxt 23 23 RNA RNA NNP cord-283998-whwksoxt 23 24 . . . cord-283998-whwksoxt 24 1 Like like IN cord-283998-whwksoxt 24 2 RSV RSV NNP cord-283998-whwksoxt 24 3 RNA RNA NNP cord-283998-whwksoxt 24 4 ( ( -LRB- cord-283998-whwksoxt 24 5 Duesberg Duesberg NNP cord-283998-whwksoxt 24 6 , , , cord-283998-whwksoxt 24 7 19681 19681 CD cord-283998-whwksoxt 24 8 , , , cord-283998-whwksoxt 24 9 the the DT cord-283998-whwksoxt 24 10 RNAs rna NNS cord-283998-whwksoxt 24 11 of of IN cord-283998-whwksoxt 24 12 TGEV TGEV NNP cord-283998-whwksoxt 24 13 and and CC cord-283998-whwksoxt 24 14 HEV HEV NNP cord-283998-whwksoxt 24 15 could could MD cord-283998-whwksoxt 24 16 be be VB cord-283998-whwksoxt 24 17 readily readily RB cord-283998-whwksoxt 24 18 dissociated dissociate VBN cord-283998-whwksoxt 24 19 to to IN cord-283998-whwksoxt 24 20 35 35 CD cord-283998-whwksoxt 24 21 S s NN cord-283998-whwksoxt 24 22 and and CC cord-283998-whwksoxt 24 23 4 4 CD cord-283998-whwksoxt 24 24 S S NNP cord-283998-whwksoxt 24 25 subunits subunit NNS cord-283998-whwksoxt 24 26 , , , cord-283998-whwksoxt 24 27 if if IN cord-283998-whwksoxt 24 28 prepared prepare VBN cord-283998-whwksoxt 24 29 by by IN cord-283998-whwksoxt 24 30 SDS sds NN cord-283998-whwksoxt 24 31 lysis lysis NN cord-283998-whwksoxt 24 32 at at IN cord-283998-whwksoxt 24 33 temperatures temperature NNS cord-283998-whwksoxt 24 34 above above IN cord-283998-whwksoxt 24 35 60 60 CD cord-283998-whwksoxt 24 36 " " '' cord-283998-whwksoxt 24 37 . . . cord-283998-whwksoxt 25 1 In in IN cord-283998-whwksoxt 25 2 the the DT cord-283998-whwksoxt 25 3 present present JJ cord-283998-whwksoxt 25 4 work work NN cord-283998-whwksoxt 25 5 , , , cord-283998-whwksoxt 25 6 we -PRON- PRP cord-283998-whwksoxt 25 7 attempt attempt VBP cord-283998-whwksoxt 25 8 to to TO cord-283998-whwksoxt 25 9 resolve resolve VB cord-283998-whwksoxt 25 10 apparent apparent JJ cord-283998-whwksoxt 25 11 differences difference NNS cord-283998-whwksoxt 25 12 in in IN cord-283998-whwksoxt 25 13 coronaviral coronaviral JJ cord-283998-whwksoxt 25 14 RNA RNA NNP cord-283998-whwksoxt 25 15 profiles profile NNS cord-283998-whwksoxt 25 16 obtained obtain VBN cord-283998-whwksoxt 25 17 by by IN cord-283998-whwksoxt 25 18 different different JJ cord-283998-whwksoxt 25 19 methods method NNS cord-283998-whwksoxt 25 20 of of IN cord-283998-whwksoxt 25 21 preparation preparation NN cord-283998-whwksoxt 25 22 , , , cord-283998-whwksoxt 25 23 using use VBG cord-283998-whwksoxt 25 24 human human JJ cord-283998-whwksoxt 25 25 coronavir coronavir NNS cord-283998-whwksoxt 25 26 - - : cord-283998-whwksoxt 25 27 us -PRON- PRP cord-283998-whwksoxt 25 28 OC-43 OC-43 NNP cord-283998-whwksoxt 25 29 as as IN cord-283998-whwksoxt 25 30 the the DT cord-283998-whwksoxt 25 31 model model NN cord-283998-whwksoxt 25 32 for for IN cord-283998-whwksoxt 25 33 study study NN cord-283998-whwksoxt 25 34 . . . cord-283998-whwksoxt 26 1 Copyright copyright NN cord-283998-whwksoxt 26 2 0 0 CD cord-283998-whwksoxt 26 3 1977 1977 CD cord-283998-whwksoxt 26 4 by by IN cord-283998-whwksoxt 26 5 Academic Academic NNP cord-283998-whwksoxt 26 6 Press Press NNP cord-283998-whwksoxt 26 7 , , , cord-283998-whwksoxt 27 1 Inc. Inc. NNP cord-283998-whwksoxt 27 2 All all DT cord-283998-whwksoxt 27 3 rights right NNS cord-283998-whwksoxt 27 4 of of IN cord-283998-whwksoxt 27 5 reproduction reproduction NN cord-283998-whwksoxt 27 6 in in IN cord-283998-whwksoxt 27 7 any any DT cord-283998-whwksoxt 27 8 form form NN cord-283998-whwksoxt 27 9 reserved reserve VBN cord-283998-whwksoxt 27 10 . . . cord-283998-whwksoxt 28 1 ISSN ISSN NNP cord-283998-whwksoxt 28 2 00426622 00426622 CD cord-283998-whwksoxt 28 3 was be VBD cord-283998-whwksoxt 28 4 first first RB cord-283998-whwksoxt 28 5 isolated isolate VBN cord-283998-whwksoxt 28 6 in in IN cord-283998-whwksoxt 28 7 human human JJ cord-283998-whwksoxt 28 8 tracheal tracheal JJ cord-283998-whwksoxt 28 9 organ organ NN cord-283998-whwksoxt 28 10 culture culture NN cord-283998-whwksoxt 28 11 , , , cord-283998-whwksoxt 28 12 but but CC cord-283998-whwksoxt 28 13 it -PRON- PRP cord-283998-whwksoxt 28 14 has have VBZ cord-283998-whwksoxt 28 15 been be VBN cord-283998-whwksoxt 28 16 adapted adapt VBN cord-283998-whwksoxt 28 17 to to TO cord-283998-whwksoxt 28 18 grow grow VB cord-283998-whwksoxt 28 19 to to IN cord-283998-whwksoxt 28 20 high high JJ cord-283998-whwksoxt 28 21 titer titer NN cord-283998-whwksoxt 28 22 in in IN cord-283998-whwksoxt 28 23 suckling suckle VBG cord-283998-whwksoxt 28 24 mouse mouse NN cord-283998-whwksoxt 28 25 brain brain NN cord-283998-whwksoxt 28 26 ( ( -LRB- cord-283998-whwksoxt 29 1 Mc Mc NNP cord-283998-whwksoxt 29 2 - - HYPH cord-283998-whwksoxt 29 3 Intosh Intosh NNP cord-283998-whwksoxt 29 4 et et NNP cord-283998-whwksoxt 29 5 al al NNP cord-283998-whwksoxt 29 6 . . NNP cord-283998-whwksoxt 29 7 , , , cord-283998-whwksoxt 29 8 1967 1967 CD cord-283998-whwksoxt 29 9 ) ) -RRB- cord-283998-whwksoxt 29 10 . . . cord-283998-whwksoxt 30 1 Unlike unlike IN cord-283998-whwksoxt 30 2 2293 2293 CD cord-283998-whwksoxt 30 3 , , , cord-283998-whwksoxt 30 4 a a DT cord-283998-whwksoxt 30 5 human human JJ cord-283998-whwksoxt 30 6 coronavirus coronavirus NN cord-283998-whwksoxt 30 7 strain strain NN cord-283998-whwksoxt 30 8 which which WDT cord-283998-whwksoxt 30 9 can can MD cord-283998-whwksoxt 30 10 be be VB cord-283998-whwksoxt 30 11 propagated propagate VBN cord-283998-whwksoxt 30 12 only only RB cord-283998-whwksoxt 30 13 in in IN cord-283998-whwksoxt 30 14 cell cell NN cord-283998-whwksoxt 30 15 culture culture NN cord-283998-whwksoxt 30 16 but but CC cord-283998-whwksoxt 30 17 is be VBZ cord-283998-whwksoxt 30 18 extremely extremely RB cord-283998-whwksoxt 30 19 labile labile JJ cord-283998-whwksoxt 30 20 ( ( -LRB- cord-283998-whwksoxt 30 21 Hierholzer Hierholzer NNP cord-283998-whwksoxt 30 22 , , , cord-283998-whwksoxt 30 23 1976 1976 CD cord-283998-whwksoxt 30 24 ) ) -RRB- cord-283998-whwksoxt 30 25 , , , cord-283998-whwksoxt 30 26 OC-43 OC-43 NNP cord-283998-whwksoxt 30 27 is be VBZ cord-283998-whwksoxt 30 28 a a DT cord-283998-whwksoxt 30 29 relatively relatively RB cord-283998-whwksoxt 30 30 stable stable JJ cord-283998-whwksoxt 30 31 virus virus NN cord-283998-whwksoxt 30 32 and and CC cord-283998-whwksoxt 30 33 possesses possess VBZ cord-283998-whwksoxt 30 34 a a DT cord-283998-whwksoxt 30 35 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 30 36 ( ( -LRB- cord-283998-whwksoxt 30 37 Kaye Kaye NNP cord-283998-whwksoxt 30 38 et et NNP cord-283998-whwksoxt 30 39 al al NNP cord-283998-whwksoxt 30 40 . . NNP cord-283998-whwksoxt 30 41 , , , cord-283998-whwksoxt 30 42 1970 1970 CD cord-283998-whwksoxt 30 43 ; ; . cord-283998-whwksoxt 31 1 Hierholzer Hierholzer NNP cord-283998-whwksoxt 31 2 et et NNP cord-283998-whwksoxt 31 3 al al NNP cord-283998-whwksoxt 31 4 . . NNP cord-283998-whwksoxt 31 5 , , , cord-283998-whwksoxt 31 6 1972 1972 CD cord-283998-whwksoxt 31 7 ) ) -RRB- cord-283998-whwksoxt 31 8 . . . cord-283998-whwksoxt 32 1 It -PRON- PRP cord-283998-whwksoxt 32 2 is be VBZ cord-283998-whwksoxt 32 3 , , , cord-283998-whwksoxt 32 4 therefore therefore RB cord-283998-whwksoxt 32 5 , , , cord-283998-whwksoxt 32 6 a a DT cord-283998-whwksoxt 32 7 more more RBR cord-283998-whwksoxt 32 8 convenient convenient JJ cord-283998-whwksoxt 32 9 model model NN cord-283998-whwksoxt 32 10 for for IN cord-283998-whwksoxt 32 11 study study NN cord-283998-whwksoxt 32 12 . . . cord-283998-whwksoxt 33 1 Solutions solution NNS cord-283998-whwksoxt 33 2 and and CC cord-283998-whwksoxt 33 3 reagents reagent NNS cord-283998-whwksoxt 33 4 . . . cord-283998-whwksoxt 34 1 The the DT cord-283998-whwksoxt 34 2 following following JJ cord-283998-whwksoxt 34 3 buffers buffer NNS cord-283998-whwksoxt 34 4 were be VBD cord-283998-whwksoxt 34 5 used use VBN cord-283998-whwksoxt 34 6 : : : cord-283998-whwksoxt 34 7 NET net NN cord-283998-whwksoxt 34 8 ( ( -LRB- cord-283998-whwksoxt 34 9 0 0 CD cord-283998-whwksoxt 34 10 . . . cord-283998-whwksoxt 35 1 Virus virus NN cord-283998-whwksoxt 35 2 was be VBD cord-283998-whwksoxt 35 3 released release VBN cord-283998-whwksoxt 35 4 from from IN cord-283998-whwksoxt 35 5 cells cell NNS cord-283998-whwksoxt 35 6 in in IN cord-283998-whwksoxt 35 7 the the DT cord-283998-whwksoxt 35 8 suspension suspension NN cord-283998-whwksoxt 35 9 by by IN cord-283998-whwksoxt 35 10 freezing freeze VBG cord-283998-whwksoxt 35 11 and and CC cord-283998-whwksoxt 35 12 thawing thaw VBG cord-283998-whwksoxt 35 13 four four CD cord-283998-whwksoxt 35 14 times time NNS cord-283998-whwksoxt 35 15 , , , cord-283998-whwksoxt 35 16 and and CC cord-283998-whwksoxt 35 17 particulate particulate NN cord-283998-whwksoxt 35 18 matter matter NN cord-283998-whwksoxt 35 19 was be VBD cord-283998-whwksoxt 35 20 removed remove VBN cord-283998-whwksoxt 35 21 by by IN cord-283998-whwksoxt 35 22 centrifuging centrifuge VBG cord-283998-whwksoxt 35 23 at at IN cord-283998-whwksoxt 35 24 5,000 5,000 CD cord-283998-whwksoxt 35 25 g g NN cord-283998-whwksoxt 35 26 in in IN cord-283998-whwksoxt 35 27 a a DT cord-283998-whwksoxt 35 28 Spinco Spinco NNP cord-283998-whwksoxt 35 29 40 40 CD cord-283998-whwksoxt 35 30 rotor rotor NN cord-283998-whwksoxt 35 31 for for IN cord-283998-whwksoxt 35 32 20 20 CD cord-283998-whwksoxt 35 33 min min NN cord-283998-whwksoxt 35 34 . . . cord-283998-whwksoxt 36 1 The the DT cord-283998-whwksoxt 36 2 supernatant supernatant NN cord-283998-whwksoxt 36 3 was be VBD cord-283998-whwksoxt 36 4 retained retain VBN cord-283998-whwksoxt 36 5 in in IN cord-283998-whwksoxt 36 6 an an DT cord-283998-whwksoxt 36 7 ice ice NN cord-283998-whwksoxt 36 8 bath bath NN cord-283998-whwksoxt 36 9 while while IN cord-283998-whwksoxt 36 10 the the DT cord-283998-whwksoxt 36 11 pellet pellet NN cord-283998-whwksoxt 36 12 was be VBD cord-283998-whwksoxt 36 13 resuspended resuspend VBN cord-283998-whwksoxt 36 14 in in IN cord-283998-whwksoxt 36 15 PBS PBS NNP cord-283998-whwksoxt 36 16 to to IN cord-283998-whwksoxt 36 17 20 20 CD cord-283998-whwksoxt 36 18 % % NN cord-283998-whwksoxt 36 19 of of IN cord-283998-whwksoxt 36 20 the the DT cord-283998-whwksoxt 36 21 original original JJ cord-283998-whwksoxt 36 22 volume volume NN cord-283998-whwksoxt 36 23 and and CC cord-283998-whwksoxt 36 24 recentrifuged recentrifuge VBN cord-283998-whwksoxt 36 25 ; ; : cord-283998-whwksoxt 36 26 both both DT cord-283998-whwksoxt 36 27 supernatants supernatant NNS cord-283998-whwksoxt 36 28 were be VBD cord-283998-whwksoxt 36 29 then then RB cord-283998-whwksoxt 36 30 combined combine VBN cord-283998-whwksoxt 36 31 for for IN cord-283998-whwksoxt 36 32 purification purification NN cord-283998-whwksoxt 36 33 . . . cord-283998-whwksoxt 37 1 Virus virus NN cord-283998-whwksoxt 37 2 was be VBD cord-283998-whwksoxt 37 3 partially partially RB cord-283998-whwksoxt 37 4 purified purify VBN cord-283998-whwksoxt 37 5 by by IN cord-283998-whwksoxt 37 6 adsorption adsorption NN cord-283998-whwksoxt 37 7 to to IN cord-283998-whwksoxt 37 8 and and CC cord-283998-whwksoxt 37 9 elution elution NN cord-283998-whwksoxt 37 10 from from IN cord-283998-whwksoxt 37 11 human human JJ cord-283998-whwksoxt 37 12 " " `` cord-283998-whwksoxt 37 13 0 0 CD cord-283998-whwksoxt 37 14 " " '' cord-283998-whwksoxt 37 15 erythrocytes erythrocyte NNS cord-283998-whwksoxt 37 16 according accord VBG cord-283998-whwksoxt 37 17 to to IN cord-283998-whwksoxt 37 18 the the DT cord-283998-whwksoxt 37 19 method method NN cord-283998-whwksoxt 37 20 of of IN cord-283998-whwksoxt 37 21 Kaye Kaye NNP cord-283998-whwksoxt 37 22 et et NNP cord-283998-whwksoxt 37 23 al al NNP cord-283998-whwksoxt 37 24 . . . cord-283998-whwksoxt 38 1 ( ( -LRB- cord-283998-whwksoxt 38 2 1970 1970 CD cord-283998-whwksoxt 38 3 ) ) -RRB- cord-283998-whwksoxt 38 4 . . . cord-283998-whwksoxt 39 1 Virus virus NN cord-283998-whwksoxt 39 2 - - HYPH cord-283998-whwksoxt 39 3 adsorbed adsorb VBN cord-283998-whwksoxt 39 4 erythrocytes erythrocyte NNS cord-283998-whwksoxt 39 5 were be VBD cord-283998-whwksoxt 39 6 washed wash VBN cord-283998-whwksoxt 39 7 three three CD cord-283998-whwksoxt 39 8 times time NNS cord-283998-whwksoxt 39 9 with with IN cord-283998-whwksoxt 39 10 ice ice NN cord-283998-whwksoxt 39 11 - - HYPH cord-283998-whwksoxt 39 12 cold cold JJ cord-283998-whwksoxt 39 13 PBS PBS NNP cord-283998-whwksoxt 39 14 , , , cord-283998-whwksoxt 39 15 and and CC cord-283998-whwksoxt 39 16 virus virus NN cord-283998-whwksoxt 39 17 was be VBD cord-283998-whwksoxt 39 18 finally finally RB cord-283998-whwksoxt 39 19 eluted elute VBN cord-283998-whwksoxt 39 20 in in IN cord-283998-whwksoxt 39 21 two two CD cord-283998-whwksoxt 39 22 steps step NNS cord-283998-whwksoxt 39 23 at at IN cord-283998-whwksoxt 39 24 37 37 CD cord-283998-whwksoxt 39 25 " " `` cord-283998-whwksoxt 39 26 into into IN cord-283998-whwksoxt 39 27 approximately approximately RB cord-283998-whwksoxt 39 28 20 20 CD cord-283998-whwksoxt 39 29 % % NN cord-283998-whwksoxt 39 30 of of IN cord-283998-whwksoxt 39 31 the the DT cord-283998-whwksoxt 39 32 original original JJ cord-283998-whwksoxt 39 33 volume volume NN cord-283998-whwksoxt 39 34 of of IN cord-283998-whwksoxt 39 35 PBS PBS NNP cord-283998-whwksoxt 39 36 . . . cord-283998-whwksoxt 40 1 In in IN cord-283998-whwksoxt 40 2 some some DT cord-283998-whwksoxt 40 3 early early JJ cord-283998-whwksoxt 40 4 purifications purification NNS cord-283998-whwksoxt 40 5 , , , cord-283998-whwksoxt 40 6 the the DT cord-283998-whwksoxt 40 7 eluate eluate NN cord-283998-whwksoxt 40 8 was be VBD cord-283998-whwksoxt 40 9 then then RB cord-283998-whwksoxt 40 10 treated treat VBN cord-283998-whwksoxt 40 11 with with IN cord-283998-whwksoxt 40 12 ribonuclease ribonuclease NN cord-283998-whwksoxt 40 13 and and CC cord-283998-whwksoxt 40 14 deoxyribonuclease deoxyribonuclease NN cord-283998-whwksoxt 40 15 ( ( -LRB- cord-283998-whwksoxt 40 16 each each DT cord-283998-whwksoxt 40 17 at at IN cord-283998-whwksoxt 40 18 50 50 CD cord-283998-whwksoxt 40 19 pg pg NN cord-283998-whwksoxt 40 20 / / SYM cord-283998-whwksoxt 40 21 ml ml CC cord-283998-whwksoxt 40 22 final final JJ cord-283998-whwksoxt 40 23 concentration concentration NN cord-283998-whwksoxt 40 24 ) ) -RRB- cord-283998-whwksoxt 40 25 in in IN cord-283998-whwksoxt 40 26 order order NN cord-283998-whwksoxt 40 27 to to TO cord-283998-whwksoxt 40 28 degrade degrade VB cord-283998-whwksoxt 40 29 any any DT cord-283998-whwksoxt 40 30 cellular cellular JJ cord-283998-whwksoxt 40 31 nucleic nucleic JJ cord-283998-whwksoxt 40 32 acids acid NNS cord-283998-whwksoxt 40 33 adherent adherent JJ cord-283998-whwksoxt 40 34 to to IN cord-283998-whwksoxt 40 35 the the DT cord-283998-whwksoxt 40 36 virus virus NN cord-283998-whwksoxt 40 37 . . . cord-283998-whwksoxt 41 1 When when WRB cord-283998-whwksoxt 41 2 later later RB cord-283998-whwksoxt 41 3 work work NN cord-283998-whwksoxt 41 4 revealed reveal VBD cord-283998-whwksoxt 41 5 that that IN cord-283998-whwksoxt 41 6 the the DT cord-283998-whwksoxt 41 7 profile profile NN cord-283998-whwksoxt 41 8 of of IN cord-283998-whwksoxt 41 9 RNA RNA NNP cord-283998-whwksoxt 41 10 extracted extract VBN cord-283998-whwksoxt 41 11 from from IN cord-283998-whwksoxt 41 12 virus virus NN cord-283998-whwksoxt 41 13 treated treat VBN cord-283998-whwksoxt 41 14 in in IN cord-283998-whwksoxt 41 15 this this DT cord-283998-whwksoxt 41 16 manner manner NN cord-283998-whwksoxt 41 17 was be VBD cord-283998-whwksoxt 41 18 similar similar JJ cord-283998-whwksoxt 41 19 to to IN cord-283998-whwksoxt 41 20 that that DT cord-283998-whwksoxt 41 21 from from IN cord-283998-whwksoxt 41 22 untreated untreated JJ cord-283998-whwksoxt 41 23 virus virus NN cord-283998-whwksoxt 41 24 , , , cord-283998-whwksoxt 41 25 we -PRON- PRP cord-283998-whwksoxt 41 26 omitted omit VBD cord-283998-whwksoxt 41 27 the the DT cord-283998-whwksoxt 41 28 step step NN cord-283998-whwksoxt 41 29 . . . cord-283998-whwksoxt 42 1 Virus virus NN cord-283998-whwksoxt 42 2 in in IN cord-283998-whwksoxt 42 3 the the DT cord-283998-whwksoxt 42 4 eluate eluate NN cord-283998-whwksoxt 42 5 was be VBD cord-283998-whwksoxt 42 6 then then RB cord-283998-whwksoxt 42 7 concentrated concentrate VBN cord-283998-whwksoxt 42 8 by by IN cord-283998-whwksoxt 42 9 centrifuging centrifuge VBG cord-283998-whwksoxt 42 10 through through IN cord-283998-whwksoxt 42 11 a a DT cord-283998-whwksoxt 42 12 15 15 CD cord-283998-whwksoxt 42 13 % % NN cord-283998-whwksoxt 42 14 sucrose sucrose NN cord-283998-whwksoxt 42 15 interface interface NN cord-283998-whwksoxt 42 16 to to IN cord-283998-whwksoxt 42 17 a a DT cord-283998-whwksoxt 42 18 0.75-ml 0.75-ml CD cord-283998-whwksoxt 42 19 cushion cushion NN cord-283998-whwksoxt 42 20 of of IN cord-283998-whwksoxt 42 21 65 65 CD cord-283998-whwksoxt 42 22 % % NN cord-283998-whwksoxt 42 23 sucrose sucrose NN cord-283998-whwksoxt 42 24 in in IN cord-283998-whwksoxt 42 25 NET net NN cord-283998-whwksoxt 42 26 at at IN cord-283998-whwksoxt 42 27 43,200 43,200 CD cord-283998-whwksoxt 42 28 g g NN cord-283998-whwksoxt 42 29 for for IN cord-283998-whwksoxt 42 30 30 30 CD cord-283998-whwksoxt 42 31 min min NN cord-283998-whwksoxt 42 32 in in IN cord-283998-whwksoxt 42 33 a a DT cord-283998-whwksoxt 42 34 Spinco Spinco NNP cord-283998-whwksoxt 42 35 SW SW NNP cord-283998-whwksoxt 42 36 36 36 CD cord-283998-whwksoxt 42 37 rotor rotor NN cord-283998-whwksoxt 42 38 . . . cord-283998-whwksoxt 43 1 The the DT cord-283998-whwksoxt 43 2 concentrates concentrate NNS cord-283998-whwksoxt 43 3 were be VBD cord-283998-whwksoxt 43 4 pooled pool VBN cord-283998-whwksoxt 43 5 , , , cord-283998-whwksoxt 43 6 diluted dilute VBN cord-283998-whwksoxt 43 7 with with IN cord-283998-whwksoxt 43 8 NET net NN cord-283998-whwksoxt 43 9 , , , cord-283998-whwksoxt 43 10 and and CC cord-283998-whwksoxt 43 11 centrifuged centrifuge VBN cord-283998-whwksoxt 43 12 to to IN cord-283998-whwksoxt 43 13 equilibrium equilibrium NN cord-283998-whwksoxt 43 14 for for IN cord-283998-whwksoxt 43 15 15 15 CD cord-283998-whwksoxt 43 16 hr hr NN cord-283998-whwksoxt 43 17 at at IN cord-283998-whwksoxt 43 18 97,lOOg 97,loog CD cord-283998-whwksoxt 43 19 through through IN cord-283998-whwksoxt 43 20 a a DT cord-283998-whwksoxt 43 21 lo lo NNP cord-283998-whwksoxt 43 22 - - HYPH cord-283998-whwksoxt 43 23 ml ml NNP cord-283998-whwksoxt 43 24 25 25 CD cord-283998-whwksoxt 43 25 - - SYM cord-283998-whwksoxt 43 26 65 65 CD cord-283998-whwksoxt 43 27 % % NN cord-283998-whwksoxt 43 28 sucrose sucrose NN cord-283998-whwksoxt 43 29 gradient gradient NN cord-283998-whwksoxt 43 30 in in IN cord-283998-whwksoxt 43 31 NET net NN cord-283998-whwksoxt 43 32 at at IN cord-283998-whwksoxt 43 33 7 7 CD cord-283998-whwksoxt 43 34 " " '' cord-283998-whwksoxt 43 35 in in IN cord-283998-whwksoxt 43 36 an an DT cord-283998-whwksoxt 43 37 SW SW NNP cord-283998-whwksoxt 43 38 36 36 CD cord-283998-whwksoxt 43 39 rotor rotor NN cord-283998-whwksoxt 43 40 . . . cord-283998-whwksoxt 44 1 Thirtydrop Thirtydrop NNP cord-283998-whwksoxt 44 2 fractions fraction NNS cord-283998-whwksoxt 44 3 were be VBD cord-283998-whwksoxt 44 4 collected collect VBN cord-283998-whwksoxt 44 5 , , , cord-283998-whwksoxt 44 6 and and CC cord-283998-whwksoxt 44 7 aliquots aliquot NNS cord-283998-whwksoxt 44 8 of of IN cord-283998-whwksoxt 44 9 each each DT cord-283998-whwksoxt 44 10 were be VBD cord-283998-whwksoxt 44 11 assayed assay VBN cord-283998-whwksoxt 44 12 for for IN cord-283998-whwksoxt 44 13 total total JJ cord-283998-whwksoxt 44 14 radioactivity radioactivity NN cord-283998-whwksoxt 44 15 , , , cord-283998-whwksoxt 44 16 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 44 17 , , , cord-283998-whwksoxt 44 18 density density NN cord-283998-whwksoxt 44 19 ( ( -LRB- cord-283998-whwksoxt 44 20 from from IN cord-283998-whwksoxt 44 21 refractive refractive JJ cord-283998-whwksoxt 44 22 index index NN cord-283998-whwksoxt 44 23 ) ) -RRB- cord-283998-whwksoxt 44 24 , , , cord-283998-whwksoxt 44 25 and and CC cord-283998-whwksoxt 44 26 absorbance absorbance NN cord-283998-whwksoxt 44 27 at at IN cord-283998-whwksoxt 44 28 280 280 CD cord-283998-whwksoxt 44 29 nm nm NN cord-283998-whwksoxt 44 30 . . . cord-283998-whwksoxt 45 1 A a DT cord-283998-whwksoxt 45 2 typical typical JJ cord-283998-whwksoxt 45 3 profile profile NN cord-283998-whwksoxt 45 4 is be VBZ cord-283998-whwksoxt 45 5 seen see VBN cord-283998-whwksoxt 45 6 in in IN cord-283998-whwksoxt 45 7 Fig Fig NNP cord-283998-whwksoxt 45 8 . . . cord-283998-whwksoxt 45 9 1A 1a NN cord-283998-whwksoxt 45 10 . . . cord-283998-whwksoxt 46 1 Two two CD cord-283998-whwksoxt 46 2 visible visible JJ cord-283998-whwksoxt 46 3 bands band NNS cord-283998-whwksoxt 46 4 are be VBP cord-283998-whwksoxt 46 5 present present JJ cord-283998-whwksoxt 46 6 : : : cord-283998-whwksoxt 46 7 ( ( -LRB- cord-283998-whwksoxt 46 8 1 1 LS cord-283998-whwksoxt 46 9 ) ) -RRB- cord-283998-whwksoxt 46 10 a a DT cord-283998-whwksoxt 46 11 lower low JJR cord-283998-whwksoxt 46 12 opalescent opalescent JJ cord-283998-whwksoxt 46 13 band band NN cord-283998-whwksoxt 46 14 in in IN cord-283998-whwksoxt 46 15 the the DT cord-283998-whwksoxt 46 16 density density NN cord-283998-whwksoxt 46 17 range range NN cord-283998-whwksoxt 46 18 1.168 1.168 CD cord-283998-whwksoxt 46 19 - - HYPH cord-283998-whwksoxt 46 20 1.202 1.202 CD cord-283998-whwksoxt 46 21 g g NN cord-283998-whwksoxt 46 22 / / SYM cord-283998-whwksoxt 46 23 ml ml NN cord-283998-whwksoxt 46 24 containing contain VBG cord-283998-whwksoxt 46 25 all all DT cord-283998-whwksoxt 46 26 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 46 27 activity activity NN cord-283998-whwksoxt 46 28 and and CC cord-283998-whwksoxt 46 29 ( ( -LRB- cord-283998-whwksoxt 46 30 2 2 LS cord-283998-whwksoxt 46 31 ) ) -RRB- cord-283998-whwksoxt 46 32 an an DT cord-283998-whwksoxt 46 33 upper upper JJ cord-283998-whwksoxt 46 34 , , , cord-283998-whwksoxt 46 35 more more RBR cord-283998-whwksoxt 46 36 flocculent flocculent JJ cord-283998-whwksoxt 46 37 band band NN cord-283998-whwksoxt 46 38 of of IN cord-283998-whwksoxt 46 39 membranous membranous JJ cord-283998-whwksoxt 46 40 material material NN cord-283998-whwksoxt 46 41 , , , cord-283998-whwksoxt 46 42 having have VBG cord-283998-whwksoxt 46 43 a a DT cord-283998-whwksoxt 46 44 density density NN cord-283998-whwksoxt 46 45 of of IN cord-283998-whwksoxt 46 46 1.074 1.074 CD cord-283998-whwksoxt 46 47 - - HYPH cord-283998-whwksoxt 46 48 1.133 1.133 CD cord-283998-whwksoxt 46 49 g g NN cord-283998-whwksoxt 46 50 / / SYM cord-283998-whwksoxt 46 51 ml ml NN cord-283998-whwksoxt 46 52 and and CC cord-283998-whwksoxt 46 53 no no DT cord-283998-whwksoxt 46 54 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 46 55 . . . cord-283998-whwksoxt 47 1 Fractions fraction NNS cord-283998-whwksoxt 47 2 6 6 CD cord-283998-whwksoxt 47 3 - - SYM cord-283998-whwksoxt 47 4 10 10 CD cord-283998-whwksoxt 47 5 containing contain VBG cord-283998-whwksoxt 47 6 the the DT cord-283998-whwksoxt 47 7 lower low JJR cord-283998-whwksoxt 47 8 band band NN cord-283998-whwksoxt 47 9 were be VBD cord-283998-whwksoxt 47 10 then then RB cord-283998-whwksoxt 47 11 pooled pool VBN cord-283998-whwksoxt 47 12 , , , cord-283998-whwksoxt 47 13 diluted dilute VBN cord-283998-whwksoxt 47 14 with with IN cord-283998-whwksoxt 47 15 NET net NN cord-283998-whwksoxt 47 16 , , , cord-283998-whwksoxt 47 17 and and CC cord-283998-whwksoxt 47 18 centrifuged centrifuge VBN cord-283998-whwksoxt 47 19 in in IN cord-283998-whwksoxt 47 20 a a DT cord-283998-whwksoxt 47 21 velocity velocity NN cord-283998-whwksoxt 47 22 gradient gradient NN cord-283998-whwksoxt 47 23 in in IN cord-283998-whwksoxt 47 24 the the DT cord-283998-whwksoxt 47 25 SW SW NNP cord-283998-whwksoxt 47 26 36 36 CD cord-283998-whwksoxt 47 27 rotor rotor NN cord-283998-whwksoxt 47 28 for for IN cord-283998-whwksoxt 47 29 1 1 CD cord-283998-whwksoxt 47 30 hr hr NN cord-283998-whwksoxt 47 31 at at IN cord-283998-whwksoxt 47 32 43,200 43,200 CD cord-283998-whwksoxt 47 33 g g NN cord-283998-whwksoxt 47 34 in in IN cord-283998-whwksoxt 47 35 a a DT cord-283998-whwksoxt 47 36 9.0ml 9.0ml CD cord-283998-whwksoxt 47 37 15 15 CD cord-283998-whwksoxt 47 38 - - SYM cord-283998-whwksoxt 47 39 65 65 CD cord-283998-whwksoxt 47 40 % % NN cord-283998-whwksoxt 47 41 sucrose sucrose NN cord-283998-whwksoxt 47 42 gradient gradient NN cord-283998-whwksoxt 47 43 in in IN cord-283998-whwksoxt 47 44 NET net NN cord-283998-whwksoxt 47 45 . . . cord-283998-whwksoxt 48 1 Fractions fraction NNS cord-283998-whwksoxt 48 2 were be VBD cord-283998-whwksoxt 48 3 collected collect VBN cord-283998-whwksoxt 48 4 and and CC cord-283998-whwksoxt 48 5 assayed assay VBN cord-283998-whwksoxt 48 6 for for IN cord-283998-whwksoxt 48 7 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 48 8 and and CC cord-283998-whwksoxt 48 9 radioactivity radioactivity NN cord-283998-whwksoxt 48 10 . . . cord-283998-whwksoxt 49 1 The the DT cord-283998-whwksoxt 49 2 profile profile NN cord-283998-whwksoxt 49 3 in in IN cord-283998-whwksoxt 49 4 Fig Fig NNP cord-283998-whwksoxt 49 5 . . . cord-283998-whwksoxt 50 1 1B 1b CD cord-283998-whwksoxt 50 2 equilibrium equilibrium NNP cord-283998-whwksoxt 51 1 centrifugation centrifugation NN cord-283998-whwksoxt 51 2 may may MD cord-283998-whwksoxt 51 3 be be VB cord-283998-whwksoxt 51 4 further further RB cord-283998-whwksoxt 51 5 resolved resolve VBN cord-283998-whwksoxt 51 6 into into IN cord-283998-whwksoxt 51 7 ( ( -LRB- cord-283998-whwksoxt 51 8 1 1 CD cord-283998-whwksoxt 51 9 ) ) -RRB- cord-283998-whwksoxt 51 10 a a DT cord-283998-whwksoxt 51 11 fast fast RB cord-283998-whwksoxt 51 12 - - HYPH cord-283998-whwksoxt 51 13 sedimenting sediment VBG cord-283998-whwksoxt 51 14 peak peak NN cord-283998-whwksoxt 51 15 with with IN cord-283998-whwksoxt 51 16 radioactivity radioactivity NN cord-283998-whwksoxt 51 17 but but CC cord-283998-whwksoxt 51 18 little little JJ cord-283998-whwksoxt 51 19 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 51 20 , , , cord-283998-whwksoxt 51 21 and and CC cord-283998-whwksoxt 51 22 ( ( -LRB- cord-283998-whwksoxt 51 23 2 2 LS cord-283998-whwksoxt 51 24 ) ) -RRB- cord-283998-whwksoxt 51 25 an an DT cord-283998-whwksoxt 51 26 upper upper JJ cord-283998-whwksoxt 51 27 heterogeneous heterogeneous JJ cord-283998-whwksoxt 51 28 peak peak NN cord-283998-whwksoxt 51 29 of of IN cord-283998-whwksoxt 51 30 coincident coincident JJ cord-283998-whwksoxt 51 31 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 51 32 and and CC cord-283998-whwksoxt 51 33 radioactivity radioactivity NN cord-283998-whwksoxt 51 34 . . . cord-283998-whwksoxt 52 1 The the DT cord-283998-whwksoxt 52 2 lower low JJR cord-283998-whwksoxt 52 3 peak peak NN cord-283998-whwksoxt 52 4 , , , cord-283998-whwksoxt 52 5 which which WDT cord-283998-whwksoxt 52 6 is be VBZ cord-283998-whwksoxt 52 7 not not RB cord-283998-whwksoxt 52 8 usually usually RB cord-283998-whwksoxt 52 9 visible visible JJ cord-283998-whwksoxt 52 10 , , , cord-283998-whwksoxt 52 11 probably probably RB cord-283998-whwksoxt 52 12 consists consist VBZ cord-283998-whwksoxt 52 13 of of IN cord-283998-whwksoxt 52 14 internal internal JJ cord-283998-whwksoxt 52 15 cell cell NN cord-283998-whwksoxt 52 16 membranes membrane NNS cord-283998-whwksoxt 52 17 and and CC cord-283998-whwksoxt 52 18 has have VBZ cord-283998-whwksoxt 52 19 been be VBN cord-283998-whwksoxt 52 20 observed observe VBN cord-283998-whwksoxt 52 21 by by IN cord-283998-whwksoxt 52 22 Pocock Pocock NNP cord-283998-whwksoxt 52 23 and and CC cord-283998-whwksoxt 52 24 Garwes Garwes NNP cord-283998-whwksoxt 52 25 ( ( -LRB- cord-283998-whwksoxt 52 26 1975 1975 CD cord-283998-whwksoxt 52 27 ) ) -RRB- cord-283998-whwksoxt 52 28 in in IN cord-283998-whwksoxt 52 29 the the DT cord-283998-whwksoxt 52 30 purification purification NN cord-283998-whwksoxt 52 31 of of IN cord-283998-whwksoxt 52 32 TGEV TGEV NNP cord-283998-whwksoxt 52 33 . . . cord-283998-whwksoxt 53 1 Heterogeneity heterogeneity NN cord-283998-whwksoxt 53 2 in in IN cord-283998-whwksoxt 53 3 the the DT cord-283998-whwksoxt 53 4 upper upper JJ cord-283998-whwksoxt 53 5 peak peak NN cord-283998-whwksoxt 53 6 is be VBZ cord-283998-whwksoxt 53 7 consistent consistent JJ cord-283998-whwksoxt 53 8 with with IN cord-283998-whwksoxt 53 9 the the DT cord-283998-whwksoxt 53 10 high high JJ cord-283998-whwksoxt 53 11 degree degree NN cord-283998-whwksoxt 53 12 of of IN cord-283998-whwksoxt 53 13 pleomorphism pleomorphism NN cord-283998-whwksoxt 53 14 observed observe VBN cord-283998-whwksoxt 53 15 for for IN cord-283998-whwksoxt 53 16 coronaviruses coronaviruse NNS cord-283998-whwksoxt 54 1 ( ( -LRB- cord-283998-whwksoxt 54 2 Almeida Almeida NNP cord-283998-whwksoxt 54 3 et et NNP cord-283998-whwksoxt 54 4 al al NNP cord-283998-whwksoxt 54 5 . . NNP cord-283998-whwksoxt 54 6 , , , cord-283998-whwksoxt 54 7 1968 1968 CD cord-283998-whwksoxt 54 8 ) ) -RRB- cord-283998-whwksoxt 54 9 . . . cord-283998-whwksoxt 55 1 Fractions fraction NNS cord-283998-whwksoxt 55 2 from from IN cord-283998-whwksoxt 55 3 the the DT cord-283998-whwksoxt 55 4 upper upper JJ cord-283998-whwksoxt 55 5 peak peak NN cord-283998-whwksoxt 55 6 , , , cord-283998-whwksoxt 55 7 which which WDT cord-283998-whwksoxt 55 8 were be VBD cord-283998-whwksoxt 55 9 shown show VBN cord-283998-whwksoxt 55 10 by by IN cord-283998-whwksoxt 55 11 electron electron NN cord-283998-whwksoxt 55 12 microscopy microscopy NN cord-283998-whwksoxt 55 13 to to TO cord-283998-whwksoxt 55 14 contain contain VB cord-283998-whwksoxt 55 15 highly highly RB cord-283998-whwksoxt 55 16 purified purify VBN cord-283998-whwksoxt 55 17 virus virus NN cord-283998-whwksoxt 55 18 , , , cord-283998-whwksoxt 55 19 were be VBD cord-283998-whwksoxt 55 20 pooled pool VBN cord-283998-whwksoxt 55 21 , , , cord-283998-whwksoxt 55 22 diluted dilute VBN cord-283998-whwksoxt 55 23 to to IN cord-283998-whwksoxt 55 24 5 5 CD cord-283998-whwksoxt 55 25 % % NN cord-283998-whwksoxt 55 26 sucrose sucrose NN cord-283998-whwksoxt 55 27 with with IN cord-283998-whwksoxt 55 28 NET net NN cord-283998-whwksoxt 55 29 , , , cord-283998-whwksoxt 55 30 and and CC cord-283998-whwksoxt 55 31 held hold VBD cord-283998-whwksoxt 55 32 at at IN cord-283998-whwksoxt 55 33 4 4 CD cord-283998-whwksoxt 55 34 " " `` cord-283998-whwksoxt 55 35 for for IN cord-283998-whwksoxt 55 36 RNA RNA NNP cord-283998-whwksoxt 55 37 analysis analysis NN cord-283998-whwksoxt 55 38 . . . cord-283998-whwksoxt 56 1 Final final JJ cord-283998-whwksoxt 56 2 virus virus NN cord-283998-whwksoxt 56 3 preparations preparation NNS cord-283998-whwksoxt 56 4 contained contain VBD cord-283998-whwksoxt 56 5 ap ap NNP cord-283998-whwksoxt 56 6 proximately proximately RB cord-283998-whwksoxt 56 7 11 11 CD cord-283998-whwksoxt 56 8 cpm cpm NNP cord-283998-whwksoxt 56 9 / / SYM cord-283998-whwksoxt 56 10 HA HA NNP cord-283998-whwksoxt 56 11 unit unit NN cord-283998-whwksoxt 56 12 and and CC cord-283998-whwksoxt 56 13 purification purification NN cord-283998-whwksoxt 56 14 resulted result VBD cord-283998-whwksoxt 56 15 in in IN cord-283998-whwksoxt 56 16 a a DT cord-283998-whwksoxt 56 17 1Cfold 1cfold CD cord-283998-whwksoxt 56 18 enrichment enrichment NN cord-283998-whwksoxt 56 19 of of IN cord-283998-whwksoxt 56 20 hemagglutinin hemagglutinin NN cord-283998-whwksoxt 56 21 per per IN cord-283998-whwksoxt 56 22 unit unit NN cord-283998-whwksoxt 56 23 of of IN cord-283998-whwksoxt 56 24 protein protein NN cord-283998-whwksoxt 56 25 . . . cord-283998-whwksoxt 57 1 RNA RNA NNP cord-283998-whwksoxt 57 2 extraction extraction NN cord-283998-whwksoxt 57 3 . . . cord-283998-whwksoxt 58 1 The the DT cord-283998-whwksoxt 58 2 following following JJ cord-283998-whwksoxt 58 3 methods method NNS cord-283998-whwksoxt 58 4 were be VBD cord-283998-whwksoxt 58 5 used use VBN cord-283998-whwksoxt 58 6 . . . cord-283998-whwksoxt 59 1 ( ( -LRB- cord-283998-whwksoxt 59 2 1 1 CD cord-283998-whwksoxt 59 3 ) ) -RRB- cord-283998-whwksoxt 60 1 In in IN cord-283998-whwksoxt 60 2 the the DT cord-283998-whwksoxt 60 3 warm warm JJ cord-283998-whwksoxt 60 4 phenol phenol NN cord-283998-whwksoxt 60 5 - - HYPH cord-283998-whwksoxt 60 6 SDS SDS NNP cord-283998-whwksoxt 60 7 ( ( -LRB- cord-283998-whwksoxt 60 8 WPS WPS NNP cord-283998-whwksoxt 60 9 ) ) -RRB- cord-283998-whwksoxt 60 10 method method NN cord-283998-whwksoxt 60 11 ( ( -LRB- cord-283998-whwksoxt 60 12 Tannock Tannock NNP cord-283998-whwksoxt 60 13 , , , cord-283998-whwksoxt 60 14 19731 19731 CD cord-283998-whwksoxt 60 15 , , , cord-283998-whwksoxt 60 16 the the DT cord-283998-whwksoxt 60 17 viral viral JJ cord-283998-whwksoxt 60 18 suspension suspension NN cord-283998-whwksoxt 60 19 was be VBD cord-283998-whwksoxt 60 20 shaken shake VBN cord-283998-whwksoxt 60 21 for for IN cord-283998-whwksoxt 60 22 10 10 CD cord-283998-whwksoxt 60 23 min min NN cord-283998-whwksoxt 60 24 at at IN cord-283998-whwksoxt 60 25 37 37 CD cord-283998-whwksoxt 60 26 " " `` cord-283998-whwksoxt 60 27 with with IN cord-283998-whwksoxt 60 28 phenol phenol NN cord-283998-whwksoxt 60 29 containing contain VBG cord-283998-whwksoxt 60 30 1 1 CD cord-283998-whwksoxt 60 31 % % NN cord-283998-whwksoxt 60 32 SDS SDS NNP cord-283998-whwksoxt 60 33 . . . cord-283998-whwksoxt 61 1 The the DT cord-283998-whwksoxt 61 2 two two CD cord-283998-whwksoxt 61 3 phases phase NNS cord-283998-whwksoxt 61 4 were be VBD cord-283998-whwksoxt 61 5 separated separate VBN cord-283998-whwksoxt 61 6 by by IN cord-283998-whwksoxt 61 7 centrifugation centrifugation NN cord-283998-whwksoxt 61 8 , , , cord-283998-whwksoxt 61 9 and and CC cord-283998-whwksoxt 61 10 the the DT cord-283998-whwksoxt 61 11 extraction extraction NN cord-283998-whwksoxt 61 12 was be VBD cord-283998-whwksoxt 61 13 repeated repeat VBN cord-283998-whwksoxt 61 14 . . . cord-283998-whwksoxt 62 1 After after IN cord-283998-whwksoxt 62 2 a a DT cord-283998-whwksoxt 62 3 suitable suitable JJ cord-283998-whwksoxt 62 4 marker marker NN cord-283998-whwksoxt 62 5 RNA RNA NNP cord-283998-whwksoxt 62 6 , , , cord-283998-whwksoxt 62 7 100 100 CD cord-283998-whwksoxt 62 8 pg pg NN cord-283998-whwksoxt 62 9 of of IN cord-283998-whwksoxt 62 10 carrier carrier NNP cord-283998-whwksoxt 62 11 RNA RNA NNP cord-283998-whwksoxt 62 12 , , , cord-283998-whwksoxt 62 13 and and CC cord-283998-whwksoxt 62 14 sodium sodium NN cord-283998-whwksoxt 62 15 acetate acetate NN cord-283998-whwksoxt 62 16 to to IN cord-283998-whwksoxt 62 17 0.15 0.15 CD cord-283998-whwksoxt 62 18 it4 it4 NNP cord-283998-whwksoxt 62 19 were be VBD cord-283998-whwksoxt 62 20 added add VBN cord-283998-whwksoxt 62 21 , , , cord-283998-whwksoxt 62 22 2 2 CD cord-283998-whwksoxt 62 23 vol vol NN cord-283998-whwksoxt 62 24 of of IN cord-283998-whwksoxt 62 25 ethanol ethanol NN cord-283998-whwksoxt 62 26 were be VBD cord-283998-whwksoxt 62 27 added add VBN cord-283998-whwksoxt 62 28 and and CC cord-283998-whwksoxt 62 29 all all DT cord-283998-whwksoxt 62 30 RNAs rna NNS cord-283998-whwksoxt 62 31 were be VBD cord-283998-whwksoxt 62 32 allowed allow VBN cord-283998-whwksoxt 62 33 to to TO cord-283998-whwksoxt 62 34 precipitate precipitate VB cord-283998-whwksoxt 62 35 for for IN cord-283998-whwksoxt 62 36 16 16 CD cord-283998-whwksoxt 62 37 hr hr NN cord-283998-whwksoxt 62 38 at at IN cord-283998-whwksoxt 62 39 -15 -15 CD cord-283998-whwksoxt 62 40 " " '' cord-283998-whwksoxt 62 41 . . . cord-283998-whwksoxt 63 1 The the DT cord-283998-whwksoxt 63 2 precipitate precipitate NN cord-283998-whwksoxt 63 3 was be VBD cord-283998-whwksoxt 63 4 collected collect VBN cord-283998-whwksoxt 63 5 by by IN cord-283998-whwksoxt 63 6 centrifuging centrifuge VBG cord-283998-whwksoxt 63 7 at at IN cord-283998-whwksoxt 63 8 43,200 43,200 CD cord-283998-whwksoxt 63 9 g g NN cord-283998-whwksoxt 63 10 for for IN cord-283998-whwksoxt 63 11 30 30 CD cord-283998-whwksoxt 63 12 min min NN cord-283998-whwksoxt 63 13 in in IN cord-283998-whwksoxt 63 14 an an DT cord-283998-whwksoxt 63 15 SW SW NNP cord-283998-whwksoxt 63 16 36 36 CD cord-283998-whwksoxt 63 17 rotor rotor NN cord-283998-whwksoxt 63 18 and and CC cord-283998-whwksoxt 63 19 then then RB cord-283998-whwksoxt 63 20 dissolved dissolve VBD cord-283998-whwksoxt 63 21 in in IN cord-283998-whwksoxt 63 22 a a DT cord-283998-whwksoxt 63 23 suitable suitable JJ cord-283998-whwksoxt 63 24 volume volume NN cord-283998-whwksoxt 63 25 of of IN cord-283998-whwksoxt 63 26 NET net NN cord-283998-whwksoxt 63 27 . . . cord-283998-whwksoxt 64 1 All all DT cord-283998-whwksoxt 64 2 RNAs rna NNS cord-283998-whwksoxt 64 3 were be VBD cord-283998-whwksoxt 64 4 stored store VBN cord-283998-whwksoxt 64 5 -70 -70 NN cord-283998-whwksoxt 64 6 " " '' cord-283998-whwksoxt 64 7 for for IN cord-283998-whwksoxt 64 8 analysis analysis NN cord-283998-whwksoxt 64 9 . . . cord-283998-whwksoxt 65 1 ( ( -LRB- cord-283998-whwksoxt 65 2 2 2 LS cord-283998-whwksoxt 65 3 ) ) -RRB- cord-283998-whwksoxt 66 1 In in IN cord-283998-whwksoxt 66 2 the the DT cord-283998-whwksoxt 66 3 SDS sds NN cord-283998-whwksoxt 66 4 - - HYPH cord-283998-whwksoxt 66 5 lysis lysis NN cord-283998-whwksoxt 66 6 ( ( -LRB- cord-283998-whwksoxt 66 7 SL SL NNP cord-283998-whwksoxt 66 8 ) ) -RRB- cord-283998-whwksoxt 66 9 method method NN cord-283998-whwksoxt 66 10 , , , cord-283998-whwksoxt 66 11 SDS SDS NNP cord-283998-whwksoxt 66 12 was be VBD cord-283998-whwksoxt 66 13 added add VBN cord-283998-whwksoxt 66 14 to to IN cord-283998-whwksoxt 66 15 1 1 CD cord-283998-whwksoxt 66 16 % % NN cord-283998-whwksoxt 66 17 ( ( -LRB- cord-283998-whwksoxt 66 18 w w NN cord-283998-whwksoxt 66 19 / / SYM cord-283998-whwksoxt 66 20 v v NN cord-283998-whwksoxt 66 21 ) ) -RRB- cord-283998-whwksoxt 66 22 to to IN cord-283998-whwksoxt 66 23 a a DT cord-283998-whwksoxt 66 24 purified purify VBN cord-283998-whwksoxt 66 25 virion virion NN cord-283998-whwksoxt 66 26 suspension suspension NN cord-283998-whwksoxt 66 27 , , , cord-283998-whwksoxt 66 28 and and CC cord-283998-whwksoxt 66 29 the the DT cord-283998-whwksoxt 66 30 mixture mixture NN cord-283998-whwksoxt 66 31 was be VBD cord-283998-whwksoxt 66 32 held hold VBN cord-283998-whwksoxt 66 33 at at IN cord-283998-whwksoxt 66 34 23 23 CD cord-283998-whwksoxt 66 35 " " `` cord-283998-whwksoxt 66 36 for for IN cord-283998-whwksoxt 66 37 15 15 CD cord-283998-whwksoxt 66 38 min min NN cord-283998-whwksoxt 66 39 . . . cord-283998-whwksoxt 67 1 The the DT cord-283998-whwksoxt 67 2 RNAs rna NNS cord-283998-whwksoxt 67 3 released release VBN cord-283998-whwksoxt 67 4 were be VBD cord-283998-whwksoxt 67 5 mixed mix VBN cord-283998-whwksoxt 67 6 with with IN cord-283998-whwksoxt 67 7 suitable suitable JJ cord-283998-whwksoxt 67 8 marker marker NN cord-283998-whwksoxt 67 9 RNA RNA NNP cord-283998-whwksoxt 67 10 and and CC cord-283998-whwksoxt 67 11 immediately immediately RB cord-283998-whwksoxt 67 12 analyzed analyze VBD cord-283998-whwksoxt 67 13 in in IN cord-283998-whwksoxt 67 14 preformed preformed JJ cord-283998-whwksoxt 67 15 sucrose sucrose NN cord-283998-whwksoxt 67 16 gradients gradient NNS cord-283998-whwksoxt 67 17 or or CC cord-283998-whwksoxt 67 18 in in IN cord-283998-whwksoxt 67 19 polyacrylamide polyacrylamide NN cord-283998-whwksoxt 67 20 gels gel NNS cord-283998-whwksoxt 67 21 . . . cord-283998-whwksoxt 68 1 ( ( -LRB- cord-283998-whwksoxt 68 2 3 3 LS cord-283998-whwksoxt 68 3 ) ) -RRB- cord-283998-whwksoxt 69 1 In in IN cord-283998-whwksoxt 69 2 the the DT cord-283998-whwksoxt 69 3 phenol phenol NN cord-283998-whwksoxt 69 4 - - HYPH cord-283998-whwksoxt 69 5 chloroform chloroform NN cord-283998-whwksoxt 69 6 ( ( -LRB- cord-283998-whwksoxt 69 7 PC pc NN cord-283998-whwksoxt 69 8 ) ) -RRB- cord-283998-whwksoxt 69 9 method method NN cord-283998-whwksoxt 69 10 for for IN cord-283998-whwksoxt 69 11 influenza influenza NN cord-283998-whwksoxt 69 12 modified modify VBN cord-283998-whwksoxt 69 13 from from IN cord-283998-whwksoxt 69 14 Pons Pons NNP cord-283998-whwksoxt 69 15 ( ( -LRB- cord-283998-whwksoxt 69 16 1975 1975 CD cord-283998-whwksoxt 69 17 ) ) -RRB- cord-283998-whwksoxt 69 18 by by IN cord-283998-whwksoxt 69 19 using use VBG cord-283998-whwksoxt 69 20 the the DT cord-283998-whwksoxt 69 21 ribonuclease ribonuclease NN cord-283998-whwksoxt 69 22 inhibitor inhibitor NN cord-283998-whwksoxt 69 23 diethyl diethyl NNP cord-283998-whwksoxt 69 24 pyrocarbonate pyrocarbonate NNP cord-283998-whwksoxt 69 25 ( ( -LRB- cord-283998-whwksoxt 69 26 DEP DEP NNP cord-283998-whwksoxt 69 27 ) ) -RRB- cord-283998-whwksoxt 69 28 in in IN cord-283998-whwksoxt 69 29 place place NN cord-283998-whwksoxt 69 30 of of IN cord-283998-whwksoxt 69 31 bentonite bentonite NN cord-283998-whwksoxt 69 32 , , , cord-283998-whwksoxt 69 33 the the DT cord-283998-whwksoxt 69 34 virus virus NN cord-283998-whwksoxt 69 35 sample sample NN cord-283998-whwksoxt 69 36 was be VBD cord-283998-whwksoxt 69 37 mixed mix VBN cord-283998-whwksoxt 69 38 with with IN cord-283998-whwksoxt 69 39 5 5 CD cord-283998-whwksoxt 69 40 % % NN cord-283998-whwksoxt 69 41 DEP dep NN cord-283998-whwksoxt 69 42 , , , cord-283998-whwksoxt 69 43 0.1 0.1 CD cord-283998-whwksoxt 69 44 vol2-mercaptoethanol vol2-mercaptoethanol NNP cord-283998-whwksoxt 69 45 , , , cord-283998-whwksoxt 69 46 1 1 CD cord-283998-whwksoxt 69 47 vol vol NN cord-283998-whwksoxt 69 48 PC pc NN cord-283998-whwksoxt 69 49 extraction extraction NN cord-283998-whwksoxt 69 50 buffer buffer NN cord-283998-whwksoxt 69 51 , , , cord-283998-whwksoxt 69 52 and and CC cord-283998-whwksoxt 69 53 1 1 CD cord-283998-whwksoxt 69 54 vol vol NN cord-283998-whwksoxt 69 55 of of IN cord-283998-whwksoxt 69 56 phenol phenol NN cord-283998-whwksoxt 69 57 : : : cord-283998-whwksoxt 69 58 chloroform chloroform NN cord-283998-whwksoxt 69 59 ( ( -LRB- cord-283998-whwksoxt 69 60 1 1 CD cord-283998-whwksoxt 69 61 : : : cord-283998-whwksoxt 69 62 l l NN cord-283998-whwksoxt 69 63 ) ) -RRB- cord-283998-whwksoxt 69 64 and and CC cord-283998-whwksoxt 69 65 shaken shake VBN cord-283998-whwksoxt 69 66 for for IN cord-283998-whwksoxt 69 67 3 3 CD cord-283998-whwksoxt 69 68 min min NN cord-283998-whwksoxt 69 69 at at IN cord-283998-whwksoxt 69 70 23 23 CD cord-283998-whwksoxt 69 71 " " '' cord-283998-whwksoxt 69 72 . . . cord-283998-whwksoxt 70 1 The the DT cord-283998-whwksoxt 70 2 phases phase NNS cord-283998-whwksoxt 70 3 were be VBD cord-283998-whwksoxt 70 4 separated separate VBN cord-283998-whwksoxt 70 5 by by IN cord-283998-whwksoxt 70 6 centrifugation centrifugation NN cord-283998-whwksoxt 70 7 , , , cord-283998-whwksoxt 70 8 and and CC cord-283998-whwksoxt 70 9 the the DT cord-283998-whwksoxt 70 10 extraction extraction NN cord-283998-whwksoxt 70 11 of of IN cord-283998-whwksoxt 70 12 the the DT cord-283998-whwksoxt 70 13 aqueous aqueous JJ cord-283998-whwksoxt 70 14 phase phase NN cord-283998-whwksoxt 70 15 was be VBD cord-283998-whwksoxt 70 16 repeated repeat VBN cord-283998-whwksoxt 70 17 . . . cord-283998-whwksoxt 71 1 Precipitation precipitation NN cord-283998-whwksoxt 71 2 in in IN cord-283998-whwksoxt 71 3 the the DT cord-283998-whwksoxt 71 4 presence presence NN cord-283998-whwksoxt 71 5 of of IN cord-283998-whwksoxt 71 6 marker marker NN cord-283998-whwksoxt 71 7 and and CC cord-283998-whwksoxt 71 8 carrier carrier NN cord-283998-whwksoxt 71 9 RNAs rna NNS cord-283998-whwksoxt 71 10 and and CC cord-283998-whwksoxt 71 11 centrifugation centrifugation NN cord-283998-whwksoxt 71 12 were be VBD cord-283998-whwksoxt 71 13 as as IN cord-283998-whwksoxt 71 14 described describe VBN cord-283998-whwksoxt 71 15 for for IN cord-283998-whwksoxt 71 16 the the DT cord-283998-whwksoxt 71 17 WPS WPS NNP cord-283998-whwksoxt 71 18 method method NN cord-283998-whwksoxt 71 19 . . . cord-283998-whwksoxt 72 1 RNA RNA NNP cord-283998-whwksoxt 72 2 sucrose sucrose NN cord-283998-whwksoxt 72 3 gradient gradient NN cord-283998-whwksoxt 72 4 analysis analysis NN cord-283998-whwksoxt 72 5 . . . cord-283998-whwksoxt 73 1 prepared prepare VBN cord-283998-whwksoxt 73 2 by by IN cord-283998-whwksoxt 73 3 phenol phenol NN cord-283998-whwksoxt 73 4 extraction extraction NN cord-283998-whwksoxt 73 5 were be VBD cord-283998-whwksoxt 73 6 centrifuged centrifuge VBN cord-283998-whwksoxt 73 7 through through IN cord-283998-whwksoxt 73 8 4.4 4.4 CD cord-283998-whwksoxt 73 9 ml ml NNS cord-283998-whwksoxt 73 10 of of IN cord-283998-whwksoxt 73 11 15 15 CD cord-283998-whwksoxt 73 12 - - SYM cord-283998-whwksoxt 73 13 30 30 CD cord-283998-whwksoxt 73 14 % % NN cord-283998-whwksoxt 73 15 sucrose sucrose NN cord-283998-whwksoxt 73 16 gradients gradient NNS cord-283998-whwksoxt 73 17 in in IN cord-283998-whwksoxt 73 18 NET net NN cord-283998-whwksoxt 73 19 in in RB cord-283998-whwksoxt 74 1 Spinco Spinco NNP cord-283998-whwksoxt 74 2 SW-50.1 sw-50.1 NN cord-283998-whwksoxt 74 3 rotor rotor NN cord-283998-whwksoxt 74 4 in in IN cord-283998-whwksoxt 74 5 a a DT cord-283998-whwksoxt 74 6 Model Model NNP cord-283998-whwksoxt 74 7 L-2 L-2 NNP cord-283998-whwksoxt 74 8 for for IN cord-283998-whwksoxt 74 9 2.25 2.25 CD cord-283998-whwksoxt 74 10 hr hr NN cord-283998-whwksoxt 74 11 at at IN cord-283998-whwksoxt 74 12 189,000 189,000 CD cord-283998-whwksoxt 74 13 g g NN cord-283998-whwksoxt 74 14 and and CC cord-283998-whwksoxt 74 15 7 7 CD cord-283998-whwksoxt 74 16 " " '' cord-283998-whwksoxt 74 17 . . . cord-283998-whwksoxt 75 1 Those those DT cord-283998-whwksoxt 75 2 prepared prepare VBN cord-283998-whwksoxt 75 3 by by IN cord-283998-whwksoxt 75 4 SDS sds NN cord-283998-whwksoxt 75 5 lysis lysis NN cord-283998-whwksoxt 75 6 were be VBD cord-283998-whwksoxt 75 7 centrifuged centrifuge VBN cord-283998-whwksoxt 75 8 in in IN cord-283998-whwksoxt 75 9 a a DT cord-283998-whwksoxt 75 10 Beckman Beckman NNP cord-283998-whwksoxt 75 11 Model Model NNP cord-283998-whwksoxt 75 12 L-4 L-4 NNP cord-283998-whwksoxt 75 13 ultracentrifuge ultracentrifuge NN cord-283998-whwksoxt 75 14 for for IN cord-283998-whwksoxt 75 15 2 2 CD cord-283998-whwksoxt 75 16 hr hr NN cord-283998-whwksoxt 75 17 at at IN cord-283998-whwksoxt 75 18 189,000 189,000 CD cord-283998-whwksoxt 75 19 g g NN cord-283998-whwksoxt 75 20 and and CC cord-283998-whwksoxt 75 21 7 7 CD cord-283998-whwksoxt 75 22 " " '' cord-283998-whwksoxt 76 1 ( ( -LRB- cord-283998-whwksoxt 76 2 Tannock Tannock NNP cord-283998-whwksoxt 76 3 and and CC cord-283998-whwksoxt 76 4 Griffith Griffith NNP cord-283998-whwksoxt 76 5 , , , cord-283998-whwksoxt 76 6 1975 1975 CD cord-283998-whwksoxt 76 7 ) ) -RRB- cord-283998-whwksoxt 76 8 . . . cord-283998-whwksoxt 77 1 Methods method NNS cord-283998-whwksoxt 77 2 Used use VBN cord-283998-whwksoxt 77 3 for for IN cord-283998-whwksoxt 77 4 Other Other NNP cord-283998-whwksoxt 77 5 Coronaviruses Coronaviruses NNP cord-283998-whwksoxt 77 6 RNA RNA NNP cord-283998-whwksoxt 77 7 was be VBD cord-283998-whwksoxt 77 8 prepared prepare VBN cord-283998-whwksoxt 77 9 from from IN cord-283998-whwksoxt 77 10 two two CD cord-283998-whwksoxt 77 11 samples sample NNS cord-283998-whwksoxt 77 12 of of IN cord-283998-whwksoxt 77 13 32P 32p CD cord-283998-whwksoxt 77 14 - - HYPH cord-283998-whwksoxt 77 15 labeled label VBN cord-283998-whwksoxt 77 16 OC-43 OC-43 NNP cord-283998-whwksoxt 77 17 virus virus NN cord-283998-whwksoxt 77 18 by by IN cord-283998-whwksoxt 77 19 the the DT cord-283998-whwksoxt 77 20 WPS WPS NNP cord-283998-whwksoxt 77 21 and and CC cord-283998-whwksoxt 77 22 SL SL NNP cord-283998-whwksoxt 77 23 methods method NNS cord-283998-whwksoxt 77 24 . . . cord-283998-whwksoxt 78 1 Profiles profile NNS cord-283998-whwksoxt 78 2 of of IN cord-283998-whwksoxt 78 3 OC-43 OC-43 NNP cord-283998-whwksoxt 78 4 and and CC cord-283998-whwksoxt 78 5 marker marker NN cord-283998-whwksoxt 78 6 RNAs rna NNS cord-283998-whwksoxt 78 7 from from IN cord-283998-whwksoxt 78 8 each each DT cord-283998-whwksoxt 78 9 gradient gradient NN cord-283998-whwksoxt 78 10 after after IN cord-283998-whwksoxt 78 11 centrifugation centrifugation NN cord-283998-whwksoxt 78 12 are be VBP cord-283998-whwksoxt 78 13 shown show VBN cord-283998-whwksoxt 78 14 in in IN cord-283998-whwksoxt 78 15 Fig Fig NNP cord-283998-whwksoxt 78 16 . . . cord-283998-whwksoxt 79 1 2 2 LS cord-283998-whwksoxt 79 2 . . . cord-283998-whwksoxt 80 1 With with IN cord-283998-whwksoxt 80 2 the the DT cord-283998-whwksoxt 80 3 WPS WPS NNP cord-283998-whwksoxt 80 4 method method NN cord-283998-whwksoxt 80 5 ( ( -LRB- cord-283998-whwksoxt 80 6 Fig Fig NNP cord-283998-whwksoxt 80 7 . . . cord-283998-whwksoxt 80 8 2A 2a CD cord-283998-whwksoxt 80 9 ) ) -RRB- cord-283998-whwksoxt 80 10 uted ute VBN cord-283998-whwksoxt 80 11 broadly broadly RB cord-283998-whwksoxt 80 12 in in IN cord-283998-whwksoxt 80 13 the the DT cord-283998-whwksoxt 80 14 15 15 CD cord-283998-whwksoxt 80 15 - - SYM cord-283998-whwksoxt 80 16 50 50 CD cord-283998-whwksoxt 80 17 S S NNP cord-283998-whwksoxt 80 18 region region NN cord-283998-whwksoxt 80 19 of of IN cord-283998-whwksoxt 80 20 the the DT cord-283998-whwksoxt 80 21 gradient gradient NN cord-283998-whwksoxt 80 22 , , , cord-283998-whwksoxt 80 23 and and CC cord-283998-whwksoxt 80 24 a a DT cord-283998-whwksoxt 80 25 smaller small JJR cord-283998-whwksoxt 80 26 quantity quantity NN cord-283998-whwksoxt 80 27 of of IN cord-283998-whwksoxt 80 28 4 4 CD cord-283998-whwksoxt 80 29 S S NNP cord-283998-whwksoxt 80 30 RNA RNA NNP cord-283998-whwksoxt 80 31 was be VBD cord-283998-whwksoxt 80 32 also also RB cord-283998-whwksoxt 80 33 present present JJ cord-283998-whwksoxt 80 34 . . . cord-283998-whwksoxt 81 1 With with IN cord-283998-whwksoxt 81 2 the the DT cord-283998-whwksoxt 81 3 SL sl NN cord-283998-whwksoxt 81 4 method method NN cord-283998-whwksoxt 81 5 ( ( -LRB- cord-283998-whwksoxt 81 6 Fig Fig NNP cord-283998-whwksoxt 81 7 . . . cord-283998-whwksoxt 81 8 2B 2B NNP cord-283998-whwksoxt 81 9 ) ) -RRB- cord-283998-whwksoxt 81 10 , , , cord-283998-whwksoxt 81 11 a a DT cord-283998-whwksoxt 81 12 single single JJ cord-283998-whwksoxt 81 13 large large JJ cord-283998-whwksoxt 81 14 homoge homoge JJ cord-283998-whwksoxt 81 15 - - HYPH cord-283998-whwksoxt 81 16 OC-43 OC-43 NNP cord-283998-whwksoxt 81 17 RNA RNA NNP cord-283998-whwksoxt 81 18 , , , cord-283998-whwksoxt 81 19 prepared prepare VBN cord-283998-whwksoxt 81 20 by by IN cord-283998-whwksoxt 81 21 the the DT cord-283998-whwksoxt 81 22 SL SL NNP cord-283998-whwksoxt 81 23 method method NN cord-283998-whwksoxt 81 24 , , , cord-283998-whwksoxt 81 25 neous neous NNP cord-283998-whwksoxt 81 26 RNA RNA NNP cord-283998-whwksoxt 81 27 was be VBD cord-283998-whwksoxt 81 28 obtained obtain VBN cord-283998-whwksoxt 81 29 with with IN cord-283998-whwksoxt 81 30 a a DT cord-283998-whwksoxt 81 31 sedimenmigrates sedimenmigrate NNS cord-283998-whwksoxt 81 32 as as IN cord-283998-whwksoxt 81 33 a a DT cord-283998-whwksoxt 81 34 homogeneous homogeneous JJ cord-283998-whwksoxt 81 35 complex complex NN cord-283998-whwksoxt 81 36 with with IN cord-283998-whwksoxt 81 37 tation tation NN cord-283998-whwksoxt 81 38 coefficient coefficient NN cord-283998-whwksoxt 81 39 identical identical JJ cord-283998-whwksoxt 81 40 to to IN cord-283998-whwksoxt 81 41 the the DT cord-283998-whwksoxt 81 42 major major JJ cord-283998-whwksoxt 81 43 an an DT cord-283998-whwksoxt 81 44 electrophoretic electrophoretic JJ cord-283998-whwksoxt 81 45 mobility mobility NN cord-283998-whwksoxt 81 46 similar similar JJ cord-283998-whwksoxt 81 47 to to IN cord-283998-whwksoxt 81 48 that that DT cord-283998-whwksoxt 82 1 ( ( -LRB- cord-283998-whwksoxt 82 2 70 70 CD cord-283998-whwksoxt 82 3 S s NN cord-283998-whwksoxt 82 4 ) ) -RRB- cord-283998-whwksoxt 82 5 component component NN cord-283998-whwksoxt 82 6 of of IN cord-283998-whwksoxt 82 7 RSV RSV NNP cord-283998-whwksoxt 82 8 RNA RNA NNP cord-283998-whwksoxt 82 9 . . . cord-283998-whwksoxt 83 1 The the DT cord-283998-whwksoxt 83 2 PC pc NN cord-283998-whwksoxt 83 3 of of IN cord-283998-whwksoxt 83 4 both both DT cord-283998-whwksoxt 83 5 45 45 CD cord-283998-whwksoxt 83 6 S s NN cord-283998-whwksoxt 83 7 ribosomal ribosomal JJ cord-283998-whwksoxt 83 8 precursor precursor NN cord-283998-whwksoxt 83 9 RNA rna NN cord-283998-whwksoxt 83 10 and and CC cord-283998-whwksoxt 83 11 method method NN cord-283998-whwksoxt 83 12 was be VBD cord-283998-whwksoxt 83 13 used use VBN cord-283998-whwksoxt 83 14 for for IN cord-283998-whwksoxt 83 15 all all DT cord-283998-whwksoxt 84 1 subsequent subsequent JJ cord-283998-whwksoxt 84 2 ex-(not ex-(not NNS cord-283998-whwksoxt 84 3 shown show VBN cord-283998-whwksoxt 84 4 in in IN cord-283998-whwksoxt 84 5 this this DT cord-283998-whwksoxt 84 6 figure figure NN cord-283998-whwksoxt 84 7 ) ) -RRB- cord-283998-whwksoxt 84 8 70 70 CD cord-283998-whwksoxt 84 9 S S NNP cord-283998-whwksoxt 84 10 RSV RSV NNP cord-283998-whwksoxt 84 11 RNA RNA NNP cord-283998-whwksoxt 84 12 . . . cord-283998-whwksoxt 85 1 tractions traction NNS cord-283998-whwksoxt 85 2 involving involve VBG cord-283998-whwksoxt 85 3 phenol phenol NN cord-283998-whwksoxt 85 4 because because IN cord-283998-whwksoxt 85 5 of of IN cord-283998-whwksoxt 85 6 the the DT cord-283998-whwksoxt 85 7 Semilogarithmic Semilogarithmic NNP cord-283998-whwksoxt 85 8 plots plot NNS cord-283998-whwksoxt 85 9 of of IN cord-283998-whwksoxt 85 10 molecular molecular JJ cord-283998-whwksoxt 85 11 weight weight NN cord-283998-whwksoxt 85 12 greater great JJR cord-283998-whwksoxt 85 13 RNA RNA NNP cord-283998-whwksoxt 85 14 yields yield NNS cord-283998-whwksoxt 85 15 obtained obtain VBN cord-283998-whwksoxt 85 16 ( ( -LRB- cord-283998-whwksoxt 85 17 Tannock Tannock NNP cord-283998-whwksoxt 85 18 versus versus IN cord-283998-whwksoxt 85 19 electrophoretic electrophoretic JJ cord-283998-whwksoxt 85 20 mobility mobility NN cord-283998-whwksoxt 85 21 from from IN cord-283998-whwksoxt 85 22 repliand repliand NN cord-283998-whwksoxt 85 23 Hierholzer Hierholzer NNP cord-283998-whwksoxt 85 24 , , , cord-283998-whwksoxt 85 25 unpublished unpublished JJ cord-283998-whwksoxt 85 26 observation observation NN cord-283998-whwksoxt 85 27 ) ) -RRB- cord-283998-whwksoxt 86 1 cate cate NNP cord-283998-whwksoxt 86 2 experiments experiment NNS cord-283998-whwksoxt 86 3 gave give VBD cord-283998-whwksoxt 86 4 a a DT cord-283998-whwksoxt 86 5 mean mean JJ cord-283998-whwksoxt 86 6 apparent apparent JJ cord-283998-whwksoxt 86 7 and and CC cord-283998-whwksoxt 86 8 the the DT cord-283998-whwksoxt 86 9 observation observation NN cord-283998-whwksoxt 86 10 ( ( -LRB- cord-283998-whwksoxt 86 11 Molloy Molloy NNP cord-283998-whwksoxt 86 12 et et NNP cord-283998-whwksoxt 86 13 al al NNP cord-283998-whwksoxt 86 14 . . NNP cord-283998-whwksoxt 86 15 , , , cord-283998-whwksoxt 86 16 1974 1974 CD cord-283998-whwksoxt 86 17 ) ) -RRB- cord-283998-whwksoxt 86 18 molecular molecular JJ cord-283998-whwksoxt 86 19 weight weight NN cord-283998-whwksoxt 86 20 of of IN cord-283998-whwksoxt 86 21 6.1 6.1 CD cord-283998-whwksoxt 86 22 x x SYM cord-283998-whwksoxt 86 23 lo6 lo6 NNS cord-283998-whwksoxt 86 24 for for IN cord-283998-whwksoxt 86 25 this this DT cord-283998-whwksoxt 86 26 undethat undethat JJ cord-283998-whwksoxt 86 27 phenol phenol NN cord-283998-whwksoxt 86 28 - - HYPH cord-283998-whwksoxt 86 29 chloroform chloroform NN cord-283998-whwksoxt 86 30 mixtures mixture NNS cord-283998-whwksoxt 87 1 congraded congrade VBN cord-283998-whwksoxt 87 2 complex complex NNP cord-283998-whwksoxt 88 1 ( ( -LRB- cord-283998-whwksoxt 88 2 Fig Fig NNP cord-283998-whwksoxt 88 3 . . NNP cord-283998-whwksoxt 88 4 4 4 CD cord-283998-whwksoxt 88 5 ) ) -RRB- cord-283998-whwksoxt 88 6 . . . cord-283998-whwksoxt 89 1 Acrylamide acrylamide NN cord-283998-whwksoxt 89 2 gels gel NNS cord-283998-whwksoxt 89 3 served serve VBD cord-283998-whwksoxt 89 4 polyadenosine polyadenosine NN cord-283998-whwksoxt 89 5 sequences sequence NNS cord-283998-whwksoxt 89 6 in in IN cord-283998-whwksoxt 89 7 RNA RNA NNP cord-283998-whwksoxt 89 8 . . . cord-283998-whwksoxt 90 1 electrophoresed electrophoresed NNP cord-283998-whwksoxt 90 2 at at IN cord-283998-whwksoxt 90 3 23 23 CD cord-283998-whwksoxt 90 4 " " `` cord-283998-whwksoxt 90 5 revealed reveal VBN cord-283998-whwksoxt 90 6 considera considera NN cord-283998-whwksoxt 90 7 - - HYPH cord-283998-whwksoxt 90 8 PAGE PAGE NNP cord-283998-whwksoxt 90 9 of of IN cord-283998-whwksoxt 90 10 OC-43RNA OC-43RNA NNP cord-283998-whwksoxt 90 11 ble ble JJ cord-283998-whwksoxt 90 12 breakdown breakdown NN cord-283998-whwksoxt 90 13 of of IN cord-283998-whwksoxt 90 14 the the DT cord-283998-whwksoxt 90 15 complex complex NN cord-283998-whwksoxt 90 16 ; ; . cord-283998-whwksoxt 91 1 this this DT cord-283998-whwksoxt 91 2 thermolability thermolability NN cord-283998-whwksoxt 91 3 of of IN cord-283998-whwksoxt 91 4 OC-43 OC-43 NNP cord-283998-whwksoxt 91 5 RNA RNA NNP cord-283998-whwksoxt 91 6 was be VBD cord-283998-whwksoxt 91 7 confirmed confirm VBN cord-283998-whwksoxt 91 8 in in IN cord-283998-whwksoxt 91 9 The the DT cord-283998-whwksoxt 91 10 profiles profile NNS cord-283998-whwksoxt 91 11 obtained obtain VBN cord-283998-whwksoxt 91 12 for for IN cord-283998-whwksoxt 91 13 OC-43 OC-43 NNP cord-283998-whwksoxt 91 14 RNA RNA NNP cord-283998-whwksoxt 91 15 by by IN cord-283998-whwksoxt 91 16 later later JJ cord-283998-whwksoxt 91 17 experiments experiment NNS cord-283998-whwksoxt 91 18 ( ( -LRB- cord-283998-whwksoxt 91 19 see see VB cord-283998-whwksoxt 91 20 Fig Fig NNP cord-283998-whwksoxt 91 21 . . NNP cord-283998-whwksoxt 91 22 5 5 CD cord-283998-whwksoxt 91 23 ) ) -RRB- cord-283998-whwksoxt 91 24 . . . cord-283998-whwksoxt 92 1 No no DT cord-283998-whwksoxt 92 2 discrete discrete JJ cord-283998-whwksoxt 92 3 sucrose sucrose NN cord-283998-whwksoxt 92 4 - - HYPH cord-283998-whwksoxt 92 5 gradient gradient NN cord-283998-whwksoxt 92 6 centrifugation centrifugation NN cord-283998-whwksoxt 92 7 were be VBD cord-283998-whwksoxt 92 8 com com NN cord-283998-whwksoxt 92 9 - - HYPH cord-283998-whwksoxt 92 10 viral viral JJ cord-283998-whwksoxt 92 11 4 4 CD cord-283998-whwksoxt 92 12 S S NNP cord-283998-whwksoxt 92 13 RNA RNA NNP cord-283998-whwksoxt 92 14 can can MD cord-283998-whwksoxt 92 15 be be VB cord-283998-whwksoxt 92 16 seen see VBN cord-283998-whwksoxt 92 17 in in IN cord-283998-whwksoxt 92 18 Fig Fig NNP cord-283998-whwksoxt 92 19 . . . cord-283998-whwksoxt 93 1 3A 3A NNP cord-283998-whwksoxt 93 2 and and CC cord-283998-whwksoxt 93 3 pared pare VBD cord-283998-whwksoxt 93 4 with with IN cord-283998-whwksoxt 93 5 those those DT cord-283998-whwksoxt 93 6 obtained obtain VBN cord-283998-whwksoxt 93 7 by by IN cord-283998-whwksoxt 93 8 PAGE page NN cord-283998-whwksoxt 93 9 . . . cord-283998-whwksoxt 94 1 Figthe Figthe NNP cord-283998-whwksoxt 94 2 firm firm NN cord-283998-whwksoxt 94 3 this this DT cord-283998-whwksoxt 94 4 with with IN cord-283998-whwksoxt 94 5 OC-43 OC-43 NNP cord-283998-whwksoxt 94 6 RNA RNA NNP cord-283998-whwksoxt 94 7 , , , cord-283998-whwksoxt 94 8 preparations preparation NNS cord-283998-whwksoxt 94 9 of of IN cord-283998-whwksoxt 94 10 OC-43 OC-43 NNP cord-283998-whwksoxt 94 11 virus virus NN cord-283998-whwksoxt 94 12 were be VBD cord-283998-whwksoxt 94 13 treated treat VBN cord-283998-whwksoxt 94 14 with with IN cord-283998-whwksoxt 94 15 1 1 CD cord-283998-whwksoxt 94 16 % % NN cord-283998-whwksoxt 94 17 SDS SDS NNP cord-283998-whwksoxt 94 18 by by IN cord-283998-whwksoxt 94 19 the the DT cord-283998-whwksoxt 94 20 SL SL NNP cord-283998-whwksoxt 94 21 method method NN cord-283998-whwksoxt 94 22 at at IN cord-283998-whwksoxt 94 23 23 23 CD cord-283998-whwksoxt 94 24 " " '' cord-283998-whwksoxt 94 25 , , , cord-283998-whwksoxt 94 26 37 37 CD cord-283998-whwksoxt 94 27 " " '' cord-283998-whwksoxt 94 28 , , , cord-283998-whwksoxt 94 29 and and CC cord-283998-whwksoxt 94 30 60 60 CD cord-283998-whwksoxt 94 31 " " '' cord-283998-whwksoxt 94 32 . . . cord-283998-whwksoxt 95 1 The the DT cord-283998-whwksoxt 95 2 distributions distribution NNS cord-283998-whwksoxt 95 3 of of IN cord-283998-whwksoxt 95 4 acid acid NN cord-283998-whwksoxt 95 5 - - HYPH cord-283998-whwksoxt 95 6 insoluble insoluble JJ cord-283998-whwksoxt 95 7 radioactivity radioactivity NN cord-283998-whwksoxt 95 8 for for IN cord-283998-whwksoxt 95 9 each each DT cord-283998-whwksoxt 95 10 gradient gradient NN cord-283998-whwksoxt 95 11 suggest suggest VBP cord-283998-whwksoxt 95 12 that that IN cord-283998-whwksoxt 95 13 at at IN cord-283998-whwksoxt 95 14 37 37 CD cord-283998-whwksoxt 95 15 there there EX cord-283998-whwksoxt 95 16 was be VBD cord-283998-whwksoxt 95 17 some some DT cord-283998-whwksoxt 95 18 breakdown breakdown NN cord-283998-whwksoxt 95 19 of of IN cord-283998-whwksoxt 95 20 the the DT cord-283998-whwksoxt 95 21 70 70 CD cord-283998-whwksoxt 95 22 S S NNP cord-283998-whwksoxt 95 23 complex complex NN cord-283998-whwksoxt 95 24 into into IN cord-283998-whwksoxt 95 25 smaller small JJR cord-283998-whwksoxt 95 26 4 4 CD cord-283998-whwksoxt 95 27 S s NN cord-283998-whwksoxt 95 28 fragments fragment NNS cord-283998-whwksoxt 95 29 , , , cord-283998-whwksoxt 95 30 and and CC cord-283998-whwksoxt 95 31 at at IN cord-283998-whwksoxt 95 32 60 60 CD cord-283998-whwksoxt 95 33 " " `` cord-283998-whwksoxt 95 34 a a DT cord-283998-whwksoxt 95 35 more more RBR cord-283998-whwksoxt 95 36 generalized generalized JJ cord-283998-whwksoxt 95 37 breakdown breakdown NN cord-283998-whwksoxt 95 38 to to IN cord-283998-whwksoxt 95 39 a a DT cord-283998-whwksoxt 95 40 range range NN cord-283998-whwksoxt 95 41 of of IN cord-283998-whwksoxt 95 42 intermediate intermediate JJ cord-283998-whwksoxt 95 43 species specie NNS cord-283998-whwksoxt 95 44 occurred occur VBD cord-283998-whwksoxt 95 45 ( ( -LRB- cord-283998-whwksoxt 95 46 Fig fig NN cord-283998-whwksoxt 95 47 . . . cord-283998-whwksoxt 95 48 5 5 CD cord-283998-whwksoxt 95 49 ) ) -RRB- cord-283998-whwksoxt 96 1 ( ( -LRB- cord-283998-whwksoxt 96 2 Repik Repik NNP cord-283998-whwksoxt 96 3 and and CC cord-283998-whwksoxt 96 4 Bishop Bishop NNP cord-283998-whwksoxt 96 5 , , , cord-283998-whwksoxt 96 6 1973 1973 CD cord-283998-whwksoxt 96 7 ) ) -RRB- cord-283998-whwksoxt 96 8 ; ; : cord-283998-whwksoxt 96 9 28 28 CD cord-283998-whwksoxt 96 10 and and CC cord-283998-whwksoxt 96 11 18 18 CD cord-283998-whwksoxt 96 12 S S NNP cord-283998-whwksoxt 96 13 ribosomal ribosomal NN cord-283998-whwksoxt 96 14 RNA RNA NNP cord-283998-whwksoxt 96 15 , , , cord-283998-whwksoxt 96 16 1.75 1.75 CD cord-283998-whwksoxt 96 17 and and CC cord-283998-whwksoxt 96 18 0.70 0.70 CD cord-283998-whwksoxt 96 19 x x SYM cord-283998-whwksoxt 96 20 106 106 CD cord-283998-whwksoxt 96 21 , , , cord-283998-whwksoxt 96 22 respectively respectively RB cord-283998-whwksoxt 96 23 ( ( -LRB- cord-283998-whwksoxt 96 24 Loening Loening NNP cord-283998-whwksoxt 96 25 , , , cord-283998-whwksoxt 96 26 1968 1968 CD cord-283998-whwksoxt 96 27 ) ) -RRB- cord-283998-whwksoxt 96 28 ; ; : cord-283998-whwksoxt 96 29 4 4 CD cord-283998-whwksoxt 96 30 S S NNP cord-283998-whwksoxt 96 31 ribosomal ribosomal NN cord-283998-whwksoxt 96 32 RNA RNA NNP cord-283998-whwksoxt 96 33 , , , cord-283998-whwksoxt 96 34 0.025 0.025 CD cord-283998-whwksoxt 96 35 x x SYM cord-283998-whwksoxt 96 36 10 10 CD cord-283998-whwksoxt 96 37 " " '' cord-283998-whwksoxt 96 38 ( ( -LRB- cord-283998-whwksoxt 96 39 Staynov Staynov NNP cord-283998-whwksoxt 96 40 et et NNP cord-283998-whwksoxt 96 41 al al NNP cord-283998-whwksoxt 96 42 . . NNP cord-283998-whwksoxt 96 43 , , , cord-283998-whwksoxt 96 44 1972 1972 CD cord-283998-whwksoxt 96 45 ) ) -RRB- cord-283998-whwksoxt 96 46 . . . cord-283998-whwksoxt 97 1 The the DT cord-283998-whwksoxt 97 2 position position NN cord-283998-whwksoxt 97 3 of of IN cord-283998-whwksoxt 97 4 the the DT cord-283998-whwksoxt 97 5 OC-43 OC-43 NNP cord-283998-whwksoxt 97 6 RNA RNA NNP cord-283998-whwksoxt 97 7 complex complex NN cord-283998-whwksoxt 97 8 released release VBN cord-283998-whwksoxt 97 9 by by IN cord-283998-whwksoxt 97 10 the the DT cord-283998-whwksoxt 97 11 SL sl NN cord-283998-whwksoxt 97 12 method method NN cord-283998-whwksoxt 97 13 is be VBZ cord-283998-whwksoxt 97 14 indicated indicate VBN cord-283998-whwksoxt 97 15 . . . cord-283998-whwksoxt 98 1 RNA RNA NNP cord-283998-whwksoxt 98 2 components component NNS cord-283998-whwksoxt 98 3 found find VBD cord-283998-whwksoxt 98 4 at at IN cord-283998-whwksoxt 98 5 the the DT cord-283998-whwksoxt 98 6 top top NN cord-283998-whwksoxt 98 7 of of IN cord-283998-whwksoxt 98 8 gradients gradient NNS cord-283998-whwksoxt 98 9 for for IN cord-283998-whwksoxt 98 10 total total JJ cord-283998-whwksoxt 98 11 radioactivity radioactivity NN cord-283998-whwksoxt 98 12 ( ( -LRB- cord-283998-whwksoxt 98 13 see see VB cord-283998-whwksoxt 98 14 Fig Fig NNP cord-283998-whwksoxt 98 15 . . . cord-283998-whwksoxt 98 16 7A 7a NN cord-283998-whwksoxt 98 17 ) ) -RRB- cord-283998-whwksoxt 98 18 . . . cord-283998-whwksoxt 99 1 The the DT cord-283998-whwksoxt 99 2 electrophoretic electrophoretic JJ cord-283998-whwksoxt 99 3 profiles profile NNS cord-283998-whwksoxt 99 4 of of IN cord-283998-whwksoxt 99 5 OC-43 OC-43 NNP cord-283998-whwksoxt 99 6 RNA RNA NNP cord-283998-whwksoxt 99 7 , , , cord-283998-whwksoxt 99 8 after after IN cord-283998-whwksoxt 99 9 preparation preparation NN cord-283998-whwksoxt 99 10 by by IN cord-283998-whwksoxt 99 11 the the DT cord-283998-whwksoxt 99 12 PC pc NN cord-283998-whwksoxt 99 13 method method NN cord-283998-whwksoxt 99 14 , , , cord-283998-whwksoxt 99 15 were be VBD cord-283998-whwksoxt 99 16 determined determine VBN cord-283998-whwksoxt 99 17 at at IN cord-283998-whwksoxt 99 18 5 5 CD cord-283998-whwksoxt 99 19 " " '' cord-283998-whwksoxt 99 20 . . . cord-283998-whwksoxt 100 1 Clearly clearly RB cord-283998-whwksoxt 100 2 , , , cord-283998-whwksoxt 100 3 the the DT cord-283998-whwksoxt 100 4 PC pc NN cord-283998-whwksoxt 100 5 method method NN cord-283998-whwksoxt 100 6 resulted result VBN cord-283998-whwksoxt 100 7 in in IN cord-283998-whwksoxt 100 8 considerable considerable JJ cord-283998-whwksoxt 100 9 breakdown breakdown NN cord-283998-whwksoxt 100 10 of of IN cord-283998-whwksoxt 100 11 the the DT cord-283998-whwksoxt 100 12 large large JJ cord-283998-whwksoxt 100 13 complex complex NN cord-283998-whwksoxt 100 14 into into IN cord-283998-whwksoxt 100 15 a a DT cord-283998-whwksoxt 100 16 range range NN cord-283998-whwksoxt 100 17 of of IN cord-283998-whwksoxt 100 18 RNA rna NN cord-283998-whwksoxt 100 19 fragments fragment NNS cord-283998-whwksoxt 100 20 with with IN cord-283998-whwksoxt 100 21 electrophoretic electrophoretic JJ cord-283998-whwksoxt 100 22 mobilities mobility NNS cord-283998-whwksoxt 100 23 between between IN cord-283998-whwksoxt 100 24 45 45 CD cord-283998-whwksoxt 100 25 S S NNP cord-283998-whwksoxt 100 26 and and CC cord-283998-whwksoxt 100 27 18 18 CD cord-283998-whwksoxt 100 28 S S NNP cord-283998-whwksoxt 100 29 ( ( -LRB- cord-283998-whwksoxt 100 30 Fig Fig NNP cord-283998-whwksoxt 100 31 . . . cord-283998-whwksoxt 100 32 3B 3B NNP cord-283998-whwksoxt 100 33 ) ) -RRB- cord-283998-whwksoxt 100 34 . . . cord-283998-whwksoxt 101 1 A a DT cord-283998-whwksoxt 101 2 minor minor JJ cord-283998-whwksoxt 101 3 4 4 CD cord-283998-whwksoxt 101 4 S s NN cord-283998-whwksoxt 101 5 component component NN cord-283998-whwksoxt 101 6 was be VBD cord-283998-whwksoxt 101 7 also also RB cord-283998-whwksoxt 101 8 present present JJ cord-283998-whwksoxt 101 9 ; ; : cord-283998-whwksoxt 101 10 it -PRON- PRP cord-283998-whwksoxt 101 11 was be VBD cord-283998-whwksoxt 101 12 similar similar JJ cord-283998-whwksoxt 101 13 to to IN cord-283998-whwksoxt 101 14 that that DT cord-283998-whwksoxt 101 15 found find VBN cord-283998-whwksoxt 101 16 for for IN cord-283998-whwksoxt 101 17 IBV IBV NNP cord-283998-whwksoxt 101 18 RNA RNA NNP cord-283998-whwksoxt 101 19 after after IN cord-283998-whwksoxt 101 20 extraction extraction NN cord-283998-whwksoxt 101 21 by by IN cord-283998-whwksoxt 101 22 the the DT cord-283998-whwksoxt 101 23 WPS WPS NNP cord-283998-whwksoxt 101 24 method method NN cord-283998-whwksoxt 101 25 ( ( -LRB- cord-283998-whwksoxt 101 26 Tannock Tannock NNP cord-283998-whwksoxt 101 27 , , , cord-283998-whwksoxt 101 28 1973 1973 CD cord-283998-whwksoxt 101 29 Garwes Garwes NNP cord-283998-whwksoxt 101 30 et et FW cord-283998-whwksoxt 101 31 al al NNP cord-283998-whwksoxt 101 32 . . . cord-283998-whwksoxt 102 1 ( ( -LRB- cord-283998-whwksoxt 102 2 1975 1975 CD cord-283998-whwksoxt 102 3 ) ) -RRB- cord-283998-whwksoxt 102 4 reported report VBD cord-283998-whwksoxt 102 5 that that IN cord-283998-whwksoxt 102 6 RNA RNA NNP cord-283998-whwksoxt 102 7 complexes complex NNS cord-283998-whwksoxt 102 8 from from IN cord-283998-whwksoxt 102 9 the the DT cord-283998-whwksoxt 102 10 porcine porcine JJ cord-283998-whwksoxt 102 11 coronaviruses coronaviruse NNS cord-283998-whwksoxt 103 1 TGEV TGEV NNP cord-283998-whwksoxt 103 2 and and CC cord-283998-whwksoxt 103 3 HEV HEV NNP cord-283998-whwksoxt 103 4 were be VBD cord-283998-whwksoxt 103 5 degraded degrade VBN cord-283998-whwksoxt 103 6 if if IN cord-283998-whwksoxt 103 7 extracted extract VBN cord-283998-whwksoxt 103 8 by by IN cord-283998-whwksoxt 103 9 SDS SDS NNP cord-283998-whwksoxt 103 10 lysis lysis NN cord-283998-whwksoxt 103 11 at at IN cord-283998-whwksoxt 103 12 temperatures temperature NNS cord-283998-whwksoxt 103 13 above above IN cord-283998-whwksoxt 103 14 60 60 CD cord-283998-whwksoxt 103 15 " " '' cord-283998-whwksoxt 103 16 , , , cord-283998-whwksoxt 103 17 as as IN cord-283998-whwksoxt 103 18 has have VBZ cord-283998-whwksoxt 103 19 been be VBN cord-283998-whwksoxt 103 20 noted note VBN cord-283998-whwksoxt 103 21 for for IN cord-283998-whwksoxt 103 22 the the DT cord-283998-whwksoxt 103 23 RNAs rna NNS cord-283998-whwksoxt 103 24 of of IN cord-283998-whwksoxt 103 25 Retroviridae Retroviridae NNP cord-283998-whwksoxt 103 26 ( ( -LRB- cord-283998-whwksoxt 103 27 Duesberg Duesberg NNP cord-283998-whwksoxt 103 28 , , , cord-283998-whwksoxt 103 29 1968 1968 CD cord-283998-whwksoxt 103 30 ) ) -RRB- cord-283998-whwksoxt 103 31 . . . cord-283998-whwksoxt 104 1 To to IN cord-283998-whwksoxt 104 2 con- con- NN cord-283998-whwksoxt 104 3 ( ( -LRB- cord-283998-whwksoxt 104 4 Duesberg Duesberg NNP cord-283998-whwksoxt 104 5 , , , cord-283998-whwksoxt 104 6 1968 1968 CD cord-283998-whwksoxt 104 7 ; ; : cord-283998-whwksoxt 104 8 Bishop Bishop NNP cord-283998-whwksoxt 104 9 et et NNP cord-283998-whwksoxt 104 10 al al NNP cord-283998-whwksoxt 104 11 . . NNP cord-283998-whwksoxt 104 12 , , , cord-283998-whwksoxt 104 13 1970a 1970a CD cord-283998-whwksoxt 104 14 , , , cord-283998-whwksoxt 104 15 b b NN cord-283998-whwksoxt 104 16 ; ; : cord-283998-whwksoxt 104 17 Baltimore Baltimore NNP cord-283998-whwksoxt 104 18 , , , cord-283998-whwksoxt 104 19 1974 1974 CD cord-283998-whwksoxt 104 20 ; ; : cord-283998-whwksoxt 104 21 King King NNP cord-283998-whwksoxt 104 22 , , , cord-283998-whwksoxt 104 23 1976 1976 CD cord-283998-whwksoxt 104 24 ) ) -RRB- cord-283998-whwksoxt 104 25 . . . cord-283998-whwksoxt 105 1 RNAs rna NNS cord-283998-whwksoxt 105 2 in in IN cord-283998-whwksoxt 105 3 each each DT cord-283998-whwksoxt 105 4 mixture mixture NN cord-283998-whwksoxt 105 5 , , , cord-283998-whwksoxt 105 6 after after IN cord-283998-whwksoxt 105 7 preparation preparation NN cord-283998-whwksoxt 105 8 , , , cord-283998-whwksoxt 105 9 were be VBD cord-283998-whwksoxt 105 10 precipitated precipitate VBN cord-283998-whwksoxt 105 11 with with IN cord-283998-whwksoxt 105 12 ethanol ethanol NN cord-283998-whwksoxt 105 13 in in IN cord-283998-whwksoxt 105 14 the the DT cord-283998-whwksoxt 105 15 presence presence NN cord-283998-whwksoxt 105 16 of of IN cord-283998-whwksoxt 105 17 carrier carrier NN cord-283998-whwksoxt 105 18 RNA RNA NNP cord-283998-whwksoxt 105 19 and and CC cord-283998-whwksoxt 105 20 analyzed analyze VBN cord-283998-whwksoxt 105 21 in in IN cord-283998-whwksoxt 105 22 sucrose sucrose NN cord-283998-whwksoxt 105 23 gradients gradient NNS cord-283998-whwksoxt 105 24 . . . cord-283998-whwksoxt 106 1 The the DT cord-283998-whwksoxt 106 2 profiles profile NNS cord-283998-whwksoxt 106 3 of of IN cord-283998-whwksoxt 106 4 acid acid NN cord-283998-whwksoxt 106 5 - - HYPH cord-283998-whwksoxt 106 6 insoluble insoluble JJ cord-283998-whwksoxt 106 7 radioactivity radioactivity NN cord-283998-whwksoxt 106 8 for for IN cord-283998-whwksoxt 106 9 each each DT cord-283998-whwksoxt 106 10 gradient gradient NN cord-283998-whwksoxt 106 11 are be VBP cord-283998-whwksoxt 106 12 shown show VBN cord-283998-whwksoxt 106 13 in in IN cord-283998-whwksoxt 106 14 Fig Fig NNP cord-283998-whwksoxt 106 15 . . . cord-283998-whwksoxt 107 1 6 6 CD cord-283998-whwksoxt 107 2 was be VBD cord-283998-whwksoxt 107 3 mixed mix VBN cord-283998-whwksoxt 107 4 with with IN cord-283998-whwksoxt 107 5 25 25 CD cord-283998-whwksoxt 107 6 ~1 ~1 CD cord-283998-whwksoxt 107 7 of of IN cord-283998-whwksoxt 107 8 NET net NN cord-283998-whwksoxt 107 9 containing contain VBG cord-283998-whwksoxt 107 10 5 5 CD cord-283998-whwksoxt 107 11 pg pg CD cord-283998-whwksoxt 107 12 of of IN cord-283998-whwksoxt 107 13 ribonuclease ribonuclease NN cord-283998-whwksoxt 107 14 for for IN cord-283998-whwksoxt 107 15 30 30 CD cord-283998-whwksoxt 107 16 min min NN cord-283998-whwksoxt 107 17 at at IN cord-283998-whwksoxt 107 18 25 25 CD cord-283998-whwksoxt 107 19 " " '' cord-283998-whwksoxt 107 20 ; ; : cord-283998-whwksoxt 107 21 the the DT cord-283998-whwksoxt 107 22 acid acid NN cord-283998-whwksoxt 107 23 - - HYPH cord-283998-whwksoxt 107 24 insoluble insoluble JJ cord-283998-whwksoxt 107 25 radioactivity radioactivity NN cord-283998-whwksoxt 107 26 remaining remain VBG cord-283998-whwksoxt 107 27 was be VBD cord-283998-whwksoxt 107 28 then then RB cord-283998-whwksoxt 107 29 determined determine VBN cord-283998-whwksoxt 107 30 after after IN cord-283998-whwksoxt 107 31 drying dry VBG cord-283998-whwksoxt 107 32 on on IN cord-283998-whwksoxt 107 33 paper paper NN cord-283998-whwksoxt 107 34 strips strip NNS cord-283998-whwksoxt 107 35 . . . cord-283998-whwksoxt 108 1 Figures figure NNS cord-283998-whwksoxt 108 2 6A 6a CD cord-283998-whwksoxt 108 3 and and CC cord-283998-whwksoxt 108 4 6B 6b NN cord-283998-whwksoxt 108 5 suggest suggest VBP cord-283998-whwksoxt 108 6 the the DT cord-283998-whwksoxt 108 7 same same JJ cord-283998-whwksoxt 108 8 heterogeneity heterogeneity NN cord-283998-whwksoxt 108 9 for for IN cord-283998-whwksoxt 108 10 the the DT cord-283998-whwksoxt 108 11 major major JJ cord-283998-whwksoxt 108 12 class class NN cord-283998-whwksoxt 108 13 of of IN cord-283998-whwksoxt 108 14 OC-43 OC-43 NNP cord-283998-whwksoxt 108 15 RNA RNA NNP cord-283998-whwksoxt 108 16 fragments fragment NNS cord-283998-whwksoxt 108 17 already already RB cord-283998-whwksoxt 108 18 noted note VBN cord-283998-whwksoxt 108 19 by by IN cord-283998-whwksoxt 108 20 the the DT cord-283998-whwksoxt 108 21 phenol phenol NN cord-283998-whwksoxt 108 22 extraction extraction NN cord-283998-whwksoxt 108 23 method method NN cord-283998-whwksoxt 108 24 ( ( -LRB- cord-283998-whwksoxt 108 25 Figs Figs NNP cord-283998-whwksoxt 108 26 . . . cord-283998-whwksoxt 108 27 2 2 CD cord-283998-whwksoxt 108 28 , , , cord-283998-whwksoxt 108 29 31 31 CD cord-283998-whwksoxt 108 30 , , , cord-283998-whwksoxt 108 31 and and CC cord-283998-whwksoxt 108 32 again again RB cord-283998-whwksoxt 108 33 confirm confirm VB cord-283998-whwksoxt 108 34 the the DT cord-283998-whwksoxt 108 35 presence presence NN cord-283998-whwksoxt 108 36 of of IN cord-283998-whwksoxt 108 37 a a DT cord-283998-whwksoxt 108 38 minor minor JJ cord-283998-whwksoxt 108 39 4 4 CD cord-283998-whwksoxt 108 40 S s NN cord-283998-whwksoxt 108 41 component component NN cord-283998-whwksoxt 108 42 . . . cord-283998-whwksoxt 109 1 Both both DT cord-283998-whwksoxt 109 2 TMV TMV NNP cord-283998-whwksoxt 109 3 and and CC cord-283998-whwksoxt 109 4 the the DT cord-283998-whwksoxt 109 5 major major JJ cord-283998-whwksoxt 109 6 70 70 CD cord-283998-whwksoxt 109 7 S S NNP cord-283998-whwksoxt 109 8 RNA RNA NNP cord-283998-whwksoxt 109 9 of of IN cord-283998-whwksoxt 109 10 RSV RSV NNP cord-283998-whwksoxt 109 11 remain remain VBP cord-283998-whwksoxt 109 12 undegraded undegraded JJ cord-283998-whwksoxt 109 13 after after IN cord-283998-whwksoxt 109 14 extraction extraction NN cord-283998-whwksoxt 109 15 . . . cord-283998-whwksoxt 110 1 This this DT cord-283998-whwksoxt 110 2 suggests suggest VBZ cord-283998-whwksoxt 110 3 either either CC cord-283998-whwksoxt 110 4 ( ( -LRB- cord-283998-whwksoxt 110 5 a a LS cord-283998-whwksoxt 110 6 ) ) -RRB- cord-283998-whwksoxt 110 7 that that IN cord-283998-whwksoxt 110 8 the the DT cord-283998-whwksoxt 110 9 heterogeneity heterogeneity NN cord-283998-whwksoxt 110 10 of of IN cord-283998-whwksoxt 110 11 the the DT cord-283998-whwksoxt 110 12 major major JJ cord-283998-whwksoxt 110 13 RNA RNA NNP cord-283998-whwksoxt 110 14 class class NN cord-283998-whwksoxt 110 15 is be VBZ cord-283998-whwksoxt 110 16 caused cause VBN cord-283998-whwksoxt 110 17 not not RB cord-283998-whwksoxt 110 18 by by IN cord-283998-whwksoxt 110 19 the the DT cord-283998-whwksoxt 110 20 activation activation NN cord-283998-whwksoxt 110 21 of of IN cord-283998-whwksoxt 110 22 ribonucleases ribonuclease NNS cord-283998-whwksoxt 110 23 but but CC cord-283998-whwksoxt 110 24 by by IN cord-283998-whwksoxt 110 25 disruption disruption NN cord-283998-whwksoxt 110 26 of of IN cord-283998-whwksoxt 110 27 noncovalent noncovalent NN cord-283998-whwksoxt 110 28 linkages linkage NNS cord-283998-whwksoxt 110 29 between between IN cord-283998-whwksoxt 110 30 RNA RNA NNP cord-283998-whwksoxt 110 31 fragments fragment NNS cord-283998-whwksoxt 110 32 which which WDT cord-283998-whwksoxt 110 33 are be VBP cord-283998-whwksoxt 110 34 much much RB cord-283998-whwksoxt 110 35 weaker weak JJR cord-283998-whwksoxt 110 36 than than IN cord-283998-whwksoxt 110 37 similar similar JJ cord-283998-whwksoxt 110 38 linkages linkage NNS cord-283998-whwksoxt 110 39 within within IN cord-283998-whwksoxt 110 40 70 70 CD cord-283998-whwksoxt 110 41 S S NNP cord-283998-whwksoxt 110 42 RSV RSV NNP cord-283998-whwksoxt 110 43 RNA RNA NNP cord-283998-whwksoxt 110 44 , , , cord-283998-whwksoxt 110 45 or or CC cord-283998-whwksoxt 110 46 ( ( -LRB- cord-283998-whwksoxt 110 47 b b LS cord-283998-whwksoxt 110 48 ) ) -RRB- cord-283998-whwksoxt 110 49 a a DT cord-283998-whwksoxt 110 50 large large JJ cord-283998-whwksoxt 110 51 RNA RNA NNP cord-283998-whwksoxt 110 52 genome genome NN cord-283998-whwksoxt 110 53 is be VBZ cord-283998-whwksoxt 110 54 extensively extensively RB cord-283998-whwksoxt 110 55 nicked nick VBN cord-283998-whwksoxt 110 56 by by IN cord-283998-whwksoxt 110 57 virion virion NN cord-283998-whwksoxt 110 58 ribonucleases ribonuclease NNS cord-283998-whwksoxt 110 59 during during IN cord-283998-whwksoxt 110 60 viral viral JJ cord-283998-whwksoxt 110 61 growth growth NN cord-283998-whwksoxt 110 62 and and CC cord-283998-whwksoxt 110 63 these these DT cord-283998-whwksoxt 110 64 enzymes enzyme NNS cord-283998-whwksoxt 110 65 are be VBP cord-283998-whwksoxt 110 66 inactivated inactivate VBN cord-283998-whwksoxt 110 67 by by IN cord-283998-whwksoxt 110 68 phenol phenol NN cord-283998-whwksoxt 110 69 extraction extraction NN cord-283998-whwksoxt 110 70 . . . cord-283998-whwksoxt 111 1 Sedimentation sedimentation NN cord-283998-whwksoxt 111 2 coefficients coefficient NNS cord-283998-whwksoxt 111 3 for for IN cord-283998-whwksoxt 111 4 the the DT cord-283998-whwksoxt 111 5 major major JJ cord-283998-whwksoxt 111 6 RNAs rna NNS cord-283998-whwksoxt 111 7 released release VBN cord-283998-whwksoxt 111 8 by by IN cord-283998-whwksoxt 111 9 phenol phenol NN cord-283998-whwksoxt 111 10 ( ( -LRB- cord-283998-whwksoxt 111 11 15 15 CD cord-283998-whwksoxt 111 12 - - SYM cord-283998-whwksoxt 111 13 55 55 CD cord-283998-whwksoxt 111 14 S S NNP cord-283998-whwksoxt 111 15 ; ; : cord-283998-whwksoxt 111 16 Fig Fig NNP cord-283998-whwksoxt 111 17 . . . cord-283998-whwksoxt 111 18 6B 6b CD cord-283998-whwksoxt 111 19 ) ) -RRB- cord-283998-whwksoxt 111 20 were be VBD cord-283998-whwksoxt 111 21 less less JJR cord-283998-whwksoxt 111 22 than than IN cord-283998-whwksoxt 111 23 those those DT cord-283998-whwksoxt 111 24 for for IN cord-283998-whwksoxt 111 25 the the DT cord-283998-whwksoxt 111 26 homogeneous homogeneous JJ cord-283998-whwksoxt 111 27 70 70 CD cord-283998-whwksoxt 111 28 S s NN cord-283998-whwksoxt 111 29 peak peak NN cord-283998-whwksoxt 111 30 obtained obtain VBN cord-283998-whwksoxt 111 31 by by IN cord-283998-whwksoxt 111 32 the the DT cord-283998-whwksoxt 111 33 SL SL NNP cord-283998-whwksoxt 111 34 method method NN cord-283998-whwksoxt 111 35 ( ( -LRB- cord-283998-whwksoxt 111 36 Fig Fig NNP cord-283998-whwksoxt 111 37 . . . cord-283998-whwksoxt 111 38 2B 2b CD cord-283998-whwksoxt 111 39 ) ) -RRB- cord-283998-whwksoxt 111 40 . . . cord-283998-whwksoxt 112 1 All all DT cord-283998-whwksoxt 112 2 32P 32p CD cord-283998-whwksoxt 112 3 - - HYPH cord-283998-whwksoxt 112 4 labeled label VBN cord-283998-whwksoxt 112 5 structures structure NNS cord-283998-whwksoxt 112 6 throughout throughout IN cord-283998-whwksoxt 112 7 the the DT cord-283998-whwksoxt 112 8 gradient gradient NN cord-283998-whwksoxt 112 9 in in IN cord-283998-whwksoxt 112 10 Fig Fig NNP cord-283998-whwksoxt 112 11 . . . cord-283998-whwksoxt 113 1 6A 6a NN cord-283998-whwksoxt 113 2 are be VBP cord-283998-whwksoxt 113 3 clearly clearly RB cord-283998-whwksoxt 113 4 RNA RNA NNP cord-283998-whwksoxt 113 5 , , , cord-283998-whwksoxt 113 6 as as IN cord-283998-whwksoxt 113 7 judged judge VBN cord-283998-whwksoxt 113 8 by by IN cord-283998-whwksoxt 113 9 their -PRON- PRP$ cord-283998-whwksoxt 113 10 uniform uniform JJ cord-283998-whwksoxt 113 11 susceptibility susceptibility NN cord-283998-whwksoxt 113 12 to to IN cord-283998-whwksoxt 113 13 ribonuclease ribonuclease NN cord-283998-whwksoxt 113 14 . . . cord-283998-whwksoxt 114 1 A a DT cord-283998-whwksoxt 114 2 similar similar JJ cord-283998-whwksoxt 114 3 experiment experiment NN cord-283998-whwksoxt 114 4 was be VBD cord-283998-whwksoxt 114 5 then then RB cord-283998-whwksoxt 114 6 carried carry VBN cord-283998-whwksoxt 114 7 out out RP cord-283998-whwksoxt 114 8 to to TO cord-283998-whwksoxt 114 9 extend extend VB cord-283998-whwksoxt 114 10 this this DT cord-283998-whwksoxt 114 11 finding finding NN cord-283998-whwksoxt 114 12 for for IN cord-283998-whwksoxt 114 13 32P 32p CD cord-283998-whwksoxt 114 14 - - HYPH cord-283998-whwksoxt 114 15 labeled label VBN cord-283998-whwksoxt 114 16 material material NN cord-283998-whwksoxt 114 17 released release VBN cord-283998-whwksoxt 114 18 by by IN cord-283998-whwksoxt 114 19 the the DT cord-283998-whwksoxt 114 20 SL SL NNP cord-283998-whwksoxt 114 21 method method NN cord-283998-whwksoxt 114 22 . . . cord-283998-whwksoxt 115 1 SL SL NNP cord-283998-whwksoxt 115 2 - - HYPH cord-283998-whwksoxt 115 3 extracted extract VBN cord-283998-whwksoxt 115 4 virus virus NN cord-283998-whwksoxt 115 5 was be VBD cord-283998-whwksoxt 115 6 centrifuged centrifuge VBN cord-283998-whwksoxt 115 7 to to TO cord-283998-whwksoxt 115 8 release release VB cord-283998-whwksoxt 115 9 the the DT cord-283998-whwksoxt 115 10 RNA RNA NNP cord-283998-whwksoxt 115 11 as as IN cord-283998-whwksoxt 115 12 a a DT cord-283998-whwksoxt 115 13 70 70 CD cord-283998-whwksoxt 115 14 S s NN cord-283998-whwksoxt 115 15 complex complex NN cord-283998-whwksoxt 115 16 ( ( -LRB- cord-283998-whwksoxt 115 17 Fig Fig NNP cord-283998-whwksoxt 115 18 . . . cord-283998-whwksoxt 115 19 2B 2B NNP cord-283998-whwksoxt 115 20 ) ) -RRB- cord-283998-whwksoxt 115 21 , , , cord-283998-whwksoxt 115 22 and and CC cord-283998-whwksoxt 115 23 half half NN cord-283998-whwksoxt 115 24 of of IN cord-283998-whwksoxt 115 25 each each DT cord-283998-whwksoxt 115 26 gradient gradient NN cord-283998-whwksoxt 115 27 fraction fraction NN cord-283998-whwksoxt 115 28 was be VBD cord-283998-whwksoxt 115 29 treated treat VBN cord-283998-whwksoxt 115 30 with with IN cord-283998-whwksoxt 115 31 ribonuclease ribonuclease NN cord-283998-whwksoxt 115 32 , , , cord-283998-whwksoxt 115 33 as as IN cord-283998-whwksoxt 115 34 above above RB cord-283998-whwksoxt 115 35 . . . cord-283998-whwksoxt 116 1 The the DT cord-283998-whwksoxt 116 2 profiles profile NNS cord-283998-whwksoxt 116 3 for for IN cord-283998-whwksoxt 116 4 acid acid NN cord-283998-whwksoxt 116 5 - - HYPH cord-283998-whwksoxt 116 6 insoluble insoluble JJ cord-283998-whwksoxt 116 7 ragradient ragradient NN cord-283998-whwksoxt 116 8 fraction fraction NN cord-283998-whwksoxt 116 9 were be VBD cord-283998-whwksoxt 116 10 assayed assay VBN cord-283998-whwksoxt 116 11 for for IN cord-283998-whwksoxt 116 12 total total JJ cord-283998-whwksoxt 116 13 radioactivity radioactivity NN cord-283998-whwksoxt 116 14 , , , cord-283998-whwksoxt 116 15 and and CC cord-283998-whwksoxt 116 16 the the DT cord-283998-whwksoxt 116 17 profile profile NN cord-283998-whwksoxt 116 18 is be VBZ cord-283998-whwksoxt 116 19 shown show VBN cord-283998-whwksoxt 116 20 in in IN cord-283998-whwksoxt 116 21 A. A. NNP cord-283998-whwksoxt 116 22 Fractions Fractions NNP cord-283998-whwksoxt 116 23 3 3 CD cord-283998-whwksoxt 116 24 - - SYM cord-283998-whwksoxt 116 25 7 7 CD cord-283998-whwksoxt 116 26 , , , cord-283998-whwksoxt 116 27 containing contain VBG cord-283998-whwksoxt 116 28 70 70 CD cord-283998-whwksoxt 116 29 S S NNP cord-283998-whwksoxt 116 30 RNA RNA NNP cord-283998-whwksoxt 116 31 , , , cord-283998-whwksoxt 116 32 were be VBD cord-283998-whwksoxt 116 33 pooled pool VBN cord-283998-whwksoxt 116 34 and and CC cord-283998-whwksoxt 116 35 reextracted reextracte VBN cord-283998-whwksoxt 116 36 in in IN cord-283998-whwksoxt 116 37 the the DT cord-283998-whwksoxt 116 38 presence presence NN cord-283998-whwksoxt 116 39 of of IN cord-283998-whwksoxt 116 40 0.1 0.1 CD cord-283998-whwksoxt 116 41 ml ml NNS cord-283998-whwksoxt 116 42 of of IN cord-283998-whwksoxt 116 43 WPS WPS NNP cord-283998-whwksoxt 116 44 - - HYPH cord-283998-whwksoxt 116 45 extracted extract VBN cord-283998-whwksoxt 116 46 13Hluridine 13hluridine CD cord-283998-whwksoxt 116 47 TMV TMV NNP cord-283998-whwksoxt 116 48 RNA RNA NNP cord-283998-whwksoxt 116 49 . . . cord-283998-whwksoxt 117 1 All all DT cord-283998-whwksoxt 117 2 RNAs rna NNS cord-283998-whwksoxt 117 3 present present JJ cord-283998-whwksoxt 117 4 were be VBD cord-283998-whwksoxt 117 5 then then RB cord-283998-whwksoxt 117 6 reprecipitated reprecipitate VBN cord-283998-whwksoxt 117 7 in in IN cord-283998-whwksoxt 117 8 ethanol ethanol NN cord-283998-whwksoxt 117 9 after after IN cord-283998-whwksoxt 117 10 100 100 CD cord-283998-whwksoxt 117 11 pg pg NN cord-283998-whwksoxt 117 12 of of IN cord-283998-whwksoxt 117 13 carrier carrier NNP cord-283998-whwksoxt 117 14 RNA RNA NNP cord-283998-whwksoxt 117 15 was be VBD cord-283998-whwksoxt 117 16 added add VBN cord-283998-whwksoxt 117 17 . . . cord-283998-whwksoxt 118 1 The the DT cord-283998-whwksoxt 118 2 precipitate precipitate NN cord-283998-whwksoxt 118 3 was be VBD cord-283998-whwksoxt 118 4 dissolved dissolve VBN cord-283998-whwksoxt 118 5 in in IN cord-283998-whwksoxt 118 6 0.5 0.5 CD cord-283998-whwksoxt 118 7 ml ml NNS cord-283998-whwksoxt 118 8 of of IN cord-283998-whwksoxt 118 9 NET net NN cord-283998-whwksoxt 118 10 and and CC cord-283998-whwksoxt 118 11 analyzed analyze VBD cord-283998-whwksoxt 118 12 in in IN cord-283998-whwksoxt 118 13 15 15 CD cord-283998-whwksoxt 118 14 - - SYM cord-283998-whwksoxt 118 15 30 30 CD cord-283998-whwksoxt 118 16 % % NN cord-283998-whwksoxt 118 17 sucrose sucrose NN cord-283998-whwksoxt 118 18 gradients gradient NNS cord-283998-whwksoxt 118 19 . . . cord-283998-whwksoxt 119 1 The the DT cord-283998-whwksoxt 119 2 profile profile NN cord-283998-whwksoxt 119 3 of of IN cord-283998-whwksoxt 119 4 acid acid NN cord-283998-whwksoxt 119 5 - - HYPH cord-283998-whwksoxt 119 6 insoluble insoluble JJ cord-283998-whwksoxt 119 7 radioactivity radioactivity NN cord-283998-whwksoxt 119 8 for for IN cord-283998-whwksoxt 119 9 and and CC cord-283998-whwksoxt 119 10 TMV TMV NNP cord-283998-whwksoxt 119 11 ( ( -LRB- cord-283998-whwksoxt 119 12 0 0 CD cord-283998-whwksoxt 119 13 - - : cord-283998-whwksoxt 119 14 --0 --0 ADD cord-283998-whwksoxt 119 15 ) ) -RRB- cord-283998-whwksoxt 119 16 RNAs rna NNS cord-283998-whwksoxt 119 17 is be VBZ cord-283998-whwksoxt 119 18 shown show VBN cord-283998-whwksoxt 119 19 in in IN cord-283998-whwksoxt 119 20 B. B. NNP cord-283998-whwksoxt 119 21 Fifty Fifty NNP cord-283998-whwksoxt 119 22 microliters microliter NNS cord-283998-whwksoxt 119 23 of of IN cord-283998-whwksoxt 119 24 a a DT cord-283998-whwksoxt 119 25 preparation preparation NN cord-283998-whwksoxt 119 26 of of IN cord-283998-whwksoxt 119 27 3ZP 3ZP NNP cord-283998-whwksoxt 119 28 - - HYPH cord-283998-whwksoxt 119 29 labeled label VBN cord-283998-whwksoxt 119 30 OC-43 OC-43 NNP cord-283998-whwksoxt 119 31 was be VBD cord-283998-whwksoxt 119 32 treated treat VBN cord-283998-whwksoxt 119 33 to to TO cord-283998-whwksoxt 119 34 release release VB cord-283998-whwksoxt 119 35 70 70 CD cord-283998-whwksoxt 119 36 S S NNP cord-283998-whwksoxt 119 37 RNA RNA NNP cord-283998-whwksoxt 119 38 by by IN cord-283998-whwksoxt 119 39 the the DT cord-283998-whwksoxt 119 40 SL SL NNP cord-283998-whwksoxt 119 41 method method NN cord-283998-whwksoxt 119 42 . . . cord-283998-whwksoxt 120 1 The the DT cord-283998-whwksoxt 120 2 preparation preparation NN cord-283998-whwksoxt 120 3 was be VBD cord-283998-whwksoxt 120 4 then then RB cord-283998-whwksoxt 120 5 centrifuged centrifuge VBN cord-283998-whwksoxt 120 6 through through IN cord-283998-whwksoxt 120 7 5 5 CD cord-283998-whwksoxt 120 8 - - SYM cord-283998-whwksoxt 120 9 20 20 CD cord-283998-whwksoxt 120 10 % % NN cord-283998-whwksoxt 120 11 sucrose sucrose NN cord-283998-whwksoxt 120 12 gradients gradient NNS cord-283998-whwksoxt 120 13 in in IN cord-283998-whwksoxt 120 14 DMSO DMSO NNP cord-283998-whwksoxt 120 15 containing contain VBG cord-283998-whwksoxt 120 16 10 10 CD cord-283998-whwksoxt 120 17 PM pm NN cord-283998-whwksoxt 120 18 LiCl LiCl NNP cord-283998-whwksoxt 120 19 for for IN cord-283998-whwksoxt 120 20 5 5 CD cord-283998-whwksoxt 120 21 hr hr NN cord-283998-whwksoxt 120 22 at at IN cord-283998-whwksoxt 120 23 189,000 189,000 CD cord-283998-whwksoxt 120 24 g g NN cord-283998-whwksoxt 120 25 and and CC cord-283998-whwksoxt 120 26 22 22 CD cord-283998-whwksoxt 120 27 " " '' cord-283998-whwksoxt 120 28 ; ; : cord-283998-whwksoxt 120 29 the the DT cord-283998-whwksoxt 120 30 profile profile NN cord-283998-whwksoxt 120 31 of of IN cord-283998-whwksoxt 120 32 total total JJ cord-283998-whwksoxt 120 33 radioactivity radioactivity NN cord-283998-whwksoxt 120 34 is be VBZ cord-283998-whwksoxt 120 35 shown show VBN cord-283998-whwksoxt 120 36 in in IN cord-283998-whwksoxt 120 37 C. C. NNP cord-283998-whwksoxt 120 38 TANNOCK TANNOCK NNP cord-283998-whwksoxt 120 39 AND and CC cord-283998-whwksoxt 120 40 HIERHOLZER HIERHOLZER NNP cord-283998-whwksoxt 120 41 dioactivity dioactivity NNP cord-283998-whwksoxt 121 1 ( ( -LRB- cord-283998-whwksoxt 121 2 Fig Fig NNP cord-283998-whwksoxt 121 3 . . . cord-283998-whwksoxt 121 4 6C 6C NNP cord-283998-whwksoxt 121 5 ) ) -RRB- cord-283998-whwksoxt 121 6 indicate indicate VBP cord-283998-whwksoxt 121 7 that that IN cord-283998-whwksoxt 121 8 the the DT cord-283998-whwksoxt 121 9 major major JJ cord-283998-whwksoxt 121 10 70 70 CD cord-283998-whwksoxt 121 11 S S NNP cord-283998-whwksoxt 121 12 complex complex NN cord-283998-whwksoxt 121 13 released release VBN cord-283998-whwksoxt 121 14 by by IN cord-283998-whwksoxt 121 15 SDS SDS NNP cord-283998-whwksoxt 121 16 lysis lysis NN cord-283998-whwksoxt 121 17 is be VBZ cord-283998-whwksoxt 121 18 fully fully RB cord-283998-whwksoxt 121 19 susceptible susceptible JJ cord-283998-whwksoxt 121 20 to to IN cord-283998-whwksoxt 121 21 ribonuclease ribonuclease NN cord-283998-whwksoxt 121 22 , , , cord-283998-whwksoxt 121 23 whereas whereas IN cord-283998-whwksoxt 121 24 the the DT cord-283998-whwksoxt 121 25 32P 32p CD cord-283998-whwksoxt 121 26 - - HYPH cord-283998-whwksoxt 121 27 labeled label VBN cord-283998-whwksoxt 121 28 material material NN cord-283998-whwksoxt 121 29 occurring occur VBG cord-283998-whwksoxt 121 30 at at IN cord-283998-whwksoxt 121 31 the the DT cord-283998-whwksoxt 121 32 top top NN cord-283998-whwksoxt 121 33 of of IN cord-283998-whwksoxt 121 34 the the DT cord-283998-whwksoxt 121 35 gradient gradient NN cord-283998-whwksoxt 121 36 remains remain VBZ cord-283998-whwksoxt 121 37 fully fully RB cord-283998-whwksoxt 121 38 resistant resistant JJ cord-283998-whwksoxt 121 39 . . . cord-283998-whwksoxt 122 1 The the DT cord-283998-whwksoxt 122 2 latter latter JJ cord-283998-whwksoxt 122 3 probably probably RB cord-283998-whwksoxt 122 4 represents represent VBZ cord-283998-whwksoxt 122 5 structural structural JJ cord-283998-whwksoxt 122 6 phospholipids phospholipid NNS cord-283998-whwksoxt 122 7 which which WDT cord-283998-whwksoxt 122 8 remain remain VBP cord-283998-whwksoxt 122 9 at at IN cord-283998-whwksoxt 122 10 the the DT cord-283998-whwksoxt 122 11 top top NN cord-283998-whwksoxt 122 12 of of IN cord-283998-whwksoxt 122 13 the the DT cord-283998-whwksoxt 122 14 gradient gradient NN cord-283998-whwksoxt 122 15 after after IN cord-283998-whwksoxt 122 16 SDS SDS NNP cord-283998-whwksoxt 122 17 lysis lysis NN cord-283998-whwksoxt 122 18 and and CC cord-283998-whwksoxt 122 19 are be VBP cord-283998-whwksoxt 122 20 distinct distinct JJ cord-283998-whwksoxt 122 21 from from IN cord-283998-whwksoxt 122 22 ribonuclease ribonuclease NN cord-283998-whwksoxt 122 23 - - HYPH cord-283998-whwksoxt 122 24 sensitive sensitive JJ cord-283998-whwksoxt 122 25 4 4 CD cord-283998-whwksoxt 122 26 S S NNP cord-283998-whwksoxt 122 27 RNA RNA NNP cord-283998-whwksoxt 122 28 obtained obtain VBN cord-283998-whwksoxt 122 29 by by IN cord-283998-whwksoxt 122 30 the the DT cord-283998-whwksoxt 122 31 PC pc NN cord-283998-whwksoxt 122 32 method method NN cord-283998-whwksoxt 122 33 ( ( -LRB- cord-283998-whwksoxt 122 34 Fig fig NN cord-283998-whwksoxt 122 35 . . . cord-283998-whwksoxt 122 36 6A 6a CD cord-283998-whwksoxt 122 37 ) ) -RRB- cord-283998-whwksoxt 122 38 . . . cord-283998-whwksoxt 123 1 of of IN cord-283998-whwksoxt 123 2 OC-43 OC-43 NNP cord-283998-whwksoxt 123 3 70 70 CD cord-283998-whwksoxt 123 4 S S NNP cord-283998-whwksoxt 123 5 RNA RNA NNP cord-283998-whwksoxt 123 6 in in IN cord-283998-whwksoxt 123 7 the the DT cord-283998-whwksoxt 123 8 Presence Presence NNP cord-283998-whwksoxt 123 9 of of IN cord-283998-whwksoxt 123 10 Phenol Phenol NNP cord-283998-whwksoxt 123 11 and and CC cord-283998-whwksoxt 124 1 DMSO DMSO NNP cord-283998-whwksoxt 124 2 Further further JJ cord-283998-whwksoxt 124 3 evidence evidence NN cord-283998-whwksoxt 124 4 that that IN cord-283998-whwksoxt 124 5 OC-43 OC-43 NNP cord-283998-whwksoxt 124 6 70 70 CD cord-283998-whwksoxt 124 7 S S NNP cord-283998-whwksoxt 124 8 RNA RNA NNP cord-283998-whwksoxt 124 9 is be VBZ cord-283998-whwksoxt 124 10 a a DT cord-283998-whwksoxt 124 11 complex complex NN cord-283998-whwksoxt 124 12 of of IN cord-283998-whwksoxt 124 13 fragments fragment NNS cord-283998-whwksoxt 124 14 held hold VBN cord-283998-whwksoxt 124 15 together together RB cord-283998-whwksoxt 124 16 by by IN cord-283998-whwksoxt 124 17 weak weak JJ cord-283998-whwksoxt 124 18 , , , cord-283998-whwksoxt 124 19 noncovalent noncovalent NN cord-283998-whwksoxt 124 20 bonds bond NNS cord-283998-whwksoxt 124 21 was be VBD cord-283998-whwksoxt 124 22 obtained obtain VBN cord-283998-whwksoxt 124 23 by by IN cord-283998-whwksoxt 124 24 ( ( -LRB- cord-283998-whwksoxt 124 25 1 1 CD cord-283998-whwksoxt 124 26 ) ) -RRB- cord-283998-whwksoxt 124 27 isolation isolation NN cord-283998-whwksoxt 124 28 of of IN cord-283998-whwksoxt 124 29 70 70 CD cord-283998-whwksoxt 124 30 S S NNP cord-283998-whwksoxt 124 31 OC-43 OC-43 NNP cord-283998-whwksoxt 124 32 RNA RNA NNP cord-283998-whwksoxt 124 33 by by IN cord-283998-whwksoxt 124 34 SDS sds NN cord-283998-whwksoxt 124 35 lysis lysis NN cord-283998-whwksoxt 124 36 and and CC cord-283998-whwksoxt 124 37 centrifugation centrifugation NN cord-283998-whwksoxt 124 38 , , , cord-283998-whwksoxt 124 39 followed follow VBN cord-283998-whwksoxt 124 40 by by IN cord-283998-whwksoxt 124 41 extraction extraction NN cord-283998-whwksoxt 124 42 by by IN cord-283998-whwksoxt 124 43 the the DT cord-283998-whwksoxt 124 44 PC pc NN cord-283998-whwksoxt 124 45 method method NN cord-283998-whwksoxt 124 46 in in IN cord-283998-whwksoxt 124 47 the the DT cord-283998-whwksoxt 124 48 presence presence NN cord-283998-whwksoxt 124 49 of of IN cord-283998-whwksoxt 124 50 TMV TMV NNP cord-283998-whwksoxt 124 51 RNA RNA NNP cord-283998-whwksoxt 124 52 , , , cord-283998-whwksoxt 124 53 and and CC cord-283998-whwksoxt 124 54 ( ( -LRB- cord-283998-whwksoxt 124 55 2 2 LS cord-283998-whwksoxt 124 56 ) ) -RRB- cord-283998-whwksoxt 124 57 isolation isolation NN cord-283998-whwksoxt 124 58 of of IN cord-283998-whwksoxt 124 59 RNA RNA NNP cord-283998-whwksoxt 124 60 by by IN cord-283998-whwksoxt 124 61 the the DT cord-283998-whwksoxt 124 62 SL SL NNP cord-283998-whwksoxt 124 63 method method NN cord-283998-whwksoxt 124 64 and and CC cord-283998-whwksoxt 124 65 centrifugation centrifugation NN cord-283998-whwksoxt 124 66 in in IN cord-283998-whwksoxt 124 67 DMSO DMSO NNP cord-283998-whwksoxt 124 68 gradients gradient NNS cord-283998-whwksoxt 124 69 . . . cord-283998-whwksoxt 125 1 OC-43 OC-43 NNP cord-283998-whwksoxt 125 2 70 70 CD cord-283998-whwksoxt 126 1 S S NNP cord-283998-whwksoxt 126 2 RNA RNA NNP cord-283998-whwksoxt 126 3 was be VBD cord-283998-whwksoxt 126 4 lysed lyse VBN cord-283998-whwksoxt 126 5 with with IN cord-283998-whwksoxt 126 6 SDS SDS NNP cord-283998-whwksoxt 126 7 and and CC cord-283998-whwksoxt 126 8 centrifuged centrifuge VBD cord-283998-whwksoxt 126 9 to to TO cord-283998-whwksoxt 126 10 isolate isolate VB cord-283998-whwksoxt 126 11 the the DT cord-283998-whwksoxt 126 12 RNA RNA NNP cord-283998-whwksoxt 126 13 complex complex NN cord-283998-whwksoxt 126 14 as as IN cord-283998-whwksoxt 126 15 described describe VBN cord-283998-whwksoxt 126 16 ( ( -LRB- cord-283998-whwksoxt 126 17 see see VB cord-283998-whwksoxt 126 18 Fig Fig NNP cord-283998-whwksoxt 126 19 . . NNP cord-283998-whwksoxt 126 20 7 7 CD cord-283998-whwksoxt 126 21 legend legend NN cord-283998-whwksoxt 126 22 ) ) -RRB- cord-283998-whwksoxt 126 23 . . . cord-283998-whwksoxt 127 1 The the DT cord-283998-whwksoxt 127 2 profile profile NN cord-283998-whwksoxt 127 3 for for IN cord-283998-whwksoxt 127 4 total total JJ cord-283998-whwksoxt 127 5 ( ( -LRB- cord-283998-whwksoxt 127 6 acid acid NN cord-283998-whwksoxt 127 7 - - HYPH cord-283998-whwksoxt 127 8 soluble soluble JJ cord-283998-whwksoxt 127 9 and and CC cord-283998-whwksoxt 127 10 -insoluble -insoluble JJ cord-283998-whwksoxt 127 11 ) ) -RRB- cord-283998-whwksoxt 127 12 radioactivity radioactivity NN cord-283998-whwksoxt 127 13 from from IN cord-283998-whwksoxt 127 14 aliquots aliquot NNS cord-283998-whwksoxt 127 15 of of IN cord-283998-whwksoxt 127 16 each each DT cord-283998-whwksoxt 127 17 gradient gradient NN cord-283998-whwksoxt 127 18 fraction fraction NN cord-283998-whwksoxt 127 19 reveals reveal VBZ cord-283998-whwksoxt 127 20 a a DT cord-283998-whwksoxt 127 21 sharp sharp JJ cord-283998-whwksoxt 127 22 peak peak NN cord-283998-whwksoxt 127 23 of of IN cord-283998-whwksoxt 127 24 32P 32p CD cord-283998-whwksoxt 127 25 radioactivity radioactivity NN cord-283998-whwksoxt 127 26 in in IN cord-283998-whwksoxt 127 27 the the DT cord-283998-whwksoxt 127 28 70 70 CD cord-283998-whwksoxt 127 29 S S NNP cord-283998-whwksoxt 127 30 region region NN cord-283998-whwksoxt 127 31 ( ( -LRB- cord-283998-whwksoxt 127 32 the the DT cord-283998-whwksoxt 127 33 RNA RNA NNP cord-283998-whwksoxt 127 34 complex complex NN cord-283998-whwksoxt 127 35 ) ) -RRB- cord-283998-whwksoxt 127 36 and and CC cord-283998-whwksoxt 127 37 a a DT cord-283998-whwksoxt 127 38 much much RB cord-283998-whwksoxt 127 39 larger large JJR cord-283998-whwksoxt 127 40 peak peak NN cord-283998-whwksoxt 127 41 of of IN cord-283998-whwksoxt 127 42 low low JJ cord-283998-whwksoxt 127 43 density density NN cord-283998-whwksoxt 127 44 material material NN cord-283998-whwksoxt 127 45 at at IN cord-283998-whwksoxt 127 46 the the DT cord-283998-whwksoxt 127 47 top top NN cord-283998-whwksoxt 127 48 of of IN cord-283998-whwksoxt 127 49 the the DT cord-283998-whwksoxt 127 50 gradient gradient NN cord-283998-whwksoxt 127 51 ( ( -LRB- cord-283998-whwksoxt 127 52 Fig Fig NNP cord-283998-whwksoxt 127 53 . . . cord-283998-whwksoxt 127 54 7A 7a NN cord-283998-whwksoxt 127 55 ) ) -RRB- cord-283998-whwksoxt 127 56 . . . cord-283998-whwksoxt 128 1 Similarly similarly RB cord-283998-whwksoxt 128 2 located located JJ cord-283998-whwksoxt 128 3 but but CC cord-283998-whwksoxt 128 4 much much RB cord-283998-whwksoxt 128 5 smaller small JJR cord-283998-whwksoxt 128 6 peaks peak NNS cord-283998-whwksoxt 128 7 for for IN cord-283998-whwksoxt 128 8 acid acid NN cord-283998-whwksoxt 128 9 - - HYPH cord-283998-whwksoxt 128 10 insoluble insoluble JJ cord-283998-whwksoxt 128 11 , , , cord-283998-whwksoxt 128 12 ribonuclease ribonuclease NN cord-283998-whwksoxt 128 13 - - HYPH cord-283998-whwksoxt 128 14 resistant resistant JJ cord-283998-whwksoxt 128 15 radioactivity radioactivity NN cord-283998-whwksoxt 128 16 are be VBP cord-283998-whwksoxt 128 17 seen see VBN cord-283998-whwksoxt 128 18 in in IN cord-283998-whwksoxt 128 19 Figs fig NNS cord-283998-whwksoxt 128 20 . . . cord-283998-whwksoxt 129 1 2B 2B NNP cord-283998-whwksoxt 129 2 , , , cord-283998-whwksoxt 129 3 5A 5a CD cord-283998-whwksoxt 129 4 , , , cord-283998-whwksoxt 129 5 and and CC cord-283998-whwksoxt 129 6 6C. 6c. CD cord-283998-whwksoxt 130 1 It -PRON- PRP cord-283998-whwksoxt 130 2 therefore therefore RB cord-283998-whwksoxt 130 3 seems seem VBZ cord-283998-whwksoxt 130 4 likely likely JJ cord-283998-whwksoxt 130 5 that that IN cord-283998-whwksoxt 130 6 SDS SDS NNP cord-283998-whwksoxt 130 7 lysis lysis NN cord-283998-whwksoxt 130 8 separates separate VBZ cord-283998-whwksoxt 130 9 RNA RNA NNP cord-283998-whwksoxt 130 10 from from IN cord-283998-whwksoxt 130 11 large large JJ cord-283998-whwksoxt 130 12 amounts amount NNS cord-283998-whwksoxt 130 13 of of IN cord-283998-whwksoxt 130 14 acid acid NN cord-283998-whwksoxt 130 15 - - HYPH cord-283998-whwksoxt 130 16 soluble soluble JJ cord-283998-whwksoxt 130 17 components component NNS cord-283998-whwksoxt 130 18 , , , cord-283998-whwksoxt 130 19 some some DT cord-283998-whwksoxt 130 20 acid acid NN cord-283998-whwksoxt 130 21 - - HYPH cord-283998-whwksoxt 130 22 insoluble insoluble JJ cord-283998-whwksoxt 130 23 phospholipids phospholipid NNS cord-283998-whwksoxt 130 24 , , , cord-283998-whwksoxt 130 25 and and CC cord-283998-whwksoxt 130 26 perhaps perhaps RB cord-283998-whwksoxt 130 27 other other JJ cord-283998-whwksoxt 130 28 components component NNS cord-283998-whwksoxt 130 29 in in IN cord-283998-whwksoxt 130 30 the the DT cord-283998-whwksoxt 130 31 outer outer JJ cord-283998-whwksoxt 130 32 virion virion NN cord-283998-whwksoxt 130 33 coat coat NN cord-283998-whwksoxt 130 34 . . . cord-283998-whwksoxt 131 1 Fractions fraction NNS cord-283998-whwksoxt 131 2 2 2 CD cord-283998-whwksoxt 131 3 - - SYM cord-283998-whwksoxt 131 4 10 10 CD cord-283998-whwksoxt 131 5 were be VBD cord-283998-whwksoxt 131 6 extracted extract VBN cord-283998-whwksoxt 131 7 by by IN cord-283998-whwksoxt 131 8 the the DT cord-283998-whwksoxt 131 9 PC pc NN cord-283998-whwksoxt 131 10 method method NN cord-283998-whwksoxt 131 11 in in IN cord-283998-whwksoxt 131 12 the the DT cord-283998-whwksoxt 131 13 presence presence NN cord-283998-whwksoxt 131 14 of of IN cord-283998-whwksoxt 131 15 13Hluridine 13Hluridine NNP cord-283998-whwksoxt 131 16 TMV TMV NNP cord-283998-whwksoxt 131 17 RNA RNA NNP cord-283998-whwksoxt 131 18 , , , cord-283998-whwksoxt 131 19 precipitated precipitate VBN cord-283998-whwksoxt 131 20 with with IN cord-283998-whwksoxt 131 21 ethanol ethanol NN cord-283998-whwksoxt 131 22 in in IN cord-283998-whwksoxt 131 23 the the DT cord-283998-whwksoxt 131 24 presence presence NN cord-283998-whwksoxt 131 25 of of IN cord-283998-whwksoxt 131 26 carrier carrier NN cord-283998-whwksoxt 131 27 RNA RNA NNP cord-283998-whwksoxt 131 28 , , , cord-283998-whwksoxt 131 29 and and CC cord-283998-whwksoxt 131 30 analyzed analyze VBD cord-283998-whwksoxt 131 31 . . . cord-283998-whwksoxt 132 1 The the DT cord-283998-whwksoxt 132 2 profile profile NN cord-283998-whwksoxt 132 3 for for IN cord-283998-whwksoxt 132 4 acid acid NN cord-283998-whwksoxt 132 5 - - HYPH cord-283998-whwksoxt 132 6 insoluble insoluble JJ cord-283998-whwksoxt 132 7 radioactivity radioactivity NN cord-283998-whwksoxt 132 8 reveals reveal VBZ cord-283998-whwksoxt 132 9 that that IN cord-283998-whwksoxt 132 10 such such JJ cord-283998-whwksoxt 132 11 treatment treatment NN cord-283998-whwksoxt 132 12 completely completely RB cord-283998-whwksoxt 132 13 degraded degrade VBD cord-283998-whwksoxt 132 14 OC-43 OC-43 NNP cord-283998-whwksoxt 132 15 70 70 CD cord-283998-whwksoxt 132 16 S S NNP cord-283998-whwksoxt 132 17 RNA RNA NNP cord-283998-whwksoxt 132 18 , , , cord-283998-whwksoxt 132 19 whereas whereas IN cord-283998-whwksoxt 132 20 TMV TMV NNP cord-283998-whwksoxt 132 21 RNA RNA NNP cord-283998-whwksoxt 132 22 remained remain VBD cord-283998-whwksoxt 132 23 intact intact JJ cord-283998-whwksoxt 132 24 ( ( -LRB- cord-283998-whwksoxt 132 25 Fig Fig NNP cord-283998-whwksoxt 132 26 . . . cord-283998-whwksoxt 132 27 7B 7B NNP cord-283998-whwksoxt 132 28 ) ) -RRB- cord-283998-whwksoxt 132 29 . . . cord-283998-whwksoxt 133 1 This this DT cord-283998-whwksoxt 133 2 experiment experiment NN cord-283998-whwksoxt 133 3 thus thus RB cord-283998-whwksoxt 133 4 confirms confirm VBZ cord-283998-whwksoxt 133 5 earlier early JJR cord-283998-whwksoxt 133 6 evidence evidence NN cord-283998-whwksoxt 133 7 that that IN cord-283998-whwksoxt 133 8 the the DT cord-283998-whwksoxt 133 9 70 70 CD cord-283998-whwksoxt 133 10 S S NNP cord-283998-whwksoxt 133 11 complex complex NN cord-283998-whwksoxt 133 12 is be VBZ cord-283998-whwksoxt 133 13 held hold VBN cord-283998-whwksoxt 133 14 together together RB cord-283998-whwksoxt 133 15 by by IN cord-283998-whwksoxt 133 16 weak weak JJ cord-283998-whwksoxt 133 17 noncovalent noncovalent NN cord-283998-whwksoxt 133 18 bonds bond NNS cord-283998-whwksoxt 133 19 which which WDT cord-283998-whwksoxt 133 20 are be VBP cord-283998-whwksoxt 133 21 destroyed destroy VBN cord-283998-whwksoxt 133 22 by by IN cord-283998-whwksoxt 133 23 phenol phenol NN cord-283998-whwksoxt 133 24 extraction extraction NN cord-283998-whwksoxt 133 25 ( ( -LRB- cord-283998-whwksoxt 133 26 Figs Figs NNP cord-283998-whwksoxt 133 27 . . . cord-283998-whwksoxt 133 28 2 2 CD cord-283998-whwksoxt 133 29 , , , cord-283998-whwksoxt 133 30 3 3 CD cord-283998-whwksoxt 133 31 ) ) -RRB- cord-283998-whwksoxt 133 32 or or CC cord-283998-whwksoxt 133 33 gentle gentle JJ cord-283998-whwksoxt 133 34 heating heating NN cord-283998-whwksoxt 133 35 ( ( -LRB- cord-283998-whwksoxt 133 36 Fig Fig NNP cord-283998-whwksoxt 133 37 . . . cord-283998-whwksoxt 133 38 5 5 CD cord-283998-whwksoxt 133 39 ) ) -RRB- cord-283998-whwksoxt 133 40 . . . cord-283998-whwksoxt 134 1 The the DT cord-283998-whwksoxt 134 2 breakdown breakdown NN cord-283998-whwksoxt 134 3 of of IN cord-283998-whwksoxt 134 4 isolated isolate VBN cord-283998-whwksoxt 134 5 70 70 CD cord-283998-whwksoxt 134 6 S S NNP cord-283998-whwksoxt 134 7 RNA RNA NNP cord-283998-whwksoxt 134 8 by by IN cord-283998-whwksoxt 134 9 the the DT cord-283998-whwksoxt 134 10 PC pc NN cord-283998-whwksoxt 134 11 extraction extraction NN cord-283998-whwksoxt 134 12 method method NN cord-283998-whwksoxt 134 13 is be VBZ cord-283998-whwksoxt 134 14 more more RBR cord-283998-whwksoxt 134 15 complete complete JJ cord-283998-whwksoxt 134 16 than than IN cord-283998-whwksoxt 134 17 by by IN cord-283998-whwksoxt 134 18 similar similar JJ cord-283998-whwksoxt 134 19 extraction extraction NN cord-283998-whwksoxt 134 20 of of IN cord-283998-whwksoxt 134 21 purified purify VBN cord-283998-whwksoxt 134 22 virions virion NNS cord-283998-whwksoxt 134 23 . . . cord-283998-whwksoxt 135 1 It -PRON- PRP cord-283998-whwksoxt 135 2 does do VBZ cord-283998-whwksoxt 135 3 not not RB cord-283998-whwksoxt 135 4 appear appear VB cord-283998-whwksoxt 135 5 to to TO cord-283998-whwksoxt 135 6 be be VB cord-283998-whwksoxt 135 7 due due JJ cord-283998-whwksoxt 135 8 to to IN cord-283998-whwksoxt 135 9 ribonuclease ribonuclease NN cord-283998-whwksoxt 135 10 activity activity NN cord-283998-whwksoxt 135 11 because because IN cord-283998-whwksoxt 135 12 TMV TMV NNP cord-283998-whwksoxt 135 13 RNA RNA NNP cord-283998-whwksoxt 135 14 within within IN cord-283998-whwksoxt 135 15 the the DT cord-283998-whwksoxt 135 16 same same JJ cord-283998-whwksoxt 135 17 mixture mixture NN cord-283998-whwksoxt 135 18 remains remain VBZ cord-283998-whwksoxt 135 19 intact intact JJ cord-283998-whwksoxt 135 20 , , , cord-283998-whwksoxt 135 21 although although IN cord-283998-whwksoxt 135 22 phenol phenol NN cord-283998-whwksoxt 135 23 extraction extraction NN cord-283998-whwksoxt 135 24 could could MD cord-283998-whwksoxt 135 25 denature denature VB cord-283998-whwksoxt 135 26 OC-43 OC-43 NNP cord-283998-whwksoxt 135 27 70 70 CD cord-283998-whwksoxt 135 28 S S NNP cord-283998-whwksoxt 135 29 RNA RNA NNP cord-283998-whwksoxt 135 30 and and CC cord-283998-whwksoxt 135 31 so so RB cord-283998-whwksoxt 135 32 reveal reveal VB cord-283998-whwksoxt 135 33 breaks break NNS cord-283998-whwksoxt 135 34 in in IN cord-283998-whwksoxt 135 35 an an DT cord-283998-whwksoxt 135 36 otherwise otherwise RB cord-283998-whwksoxt 135 37 intact intact JJ cord-283998-whwksoxt 135 38 large large JJ cord-283998-whwksoxt 135 39 RNA RNA NNP cord-283998-whwksoxt 135 40 molecule molecule NN cord-283998-whwksoxt 135 41 which which WDT cord-283998-whwksoxt 135 42 were be VBD cord-283998-whwksoxt 135 43 produced produce VBN cord-283998-whwksoxt 135 44 by by IN cord-283998-whwksoxt 135 45 such such JJ cord-283998-whwksoxt 135 46 activity activity NN cord-283998-whwksoxt 135 47 . . . cord-283998-whwksoxt 136 1 Since since IN cord-283998-whwksoxt 136 2 DMSO DMSO NNP cord-283998-whwksoxt 136 3 has have VBZ cord-283998-whwksoxt 136 4 been be VBN cord-283998-whwksoxt 136 5 used use VBN cord-283998-whwksoxt 136 6 as as IN cord-283998-whwksoxt 136 7 a a DT cord-283998-whwksoxt 136 8 critical critical JJ cord-283998-whwksoxt 136 9 test test NN cord-283998-whwksoxt 136 10 for for IN cord-283998-whwksoxt 136 11 noncovalent noncovalent NN cord-283998-whwksoxt 136 12 linkages linkage NNS cord-283998-whwksoxt 136 13 in in IN cord-283998-whwksoxt 136 14 RSV RSV NNP cord-283998-whwksoxt 136 15 70 70 CD cord-283998-whwksoxt 136 16 S S NNP cord-283998-whwksoxt 136 17 RNA RNA NNP cord-283998-whwksoxt 136 18 ( ( -LRB- cord-283998-whwksoxt 136 19 Duesberg Duesberg NNP cord-283998-whwksoxt 136 20 , , , cord-283998-whwksoxt 136 21 1968 1968 CD cord-283998-whwksoxt 136 22 ) ) -RRB- cord-283998-whwksoxt 136 23 , , , cord-283998-whwksoxt 136 24 we -PRON- PRP cord-283998-whwksoxt 136 25 investigated investigate VBD cord-283998-whwksoxt 136 26 its -PRON- PRP$ cord-283998-whwksoxt 136 27 effect effect NN cord-283998-whwksoxt 136 28 on on IN cord-283998-whwksoxt 136 29 70 70 CD cord-283998-whwksoxt 136 30 S S NNP cord-283998-whwksoxt 136 31 OC-43 OC-43 NNP cord-283998-whwksoxt 136 32 RNA RNA NNP cord-283998-whwksoxt 136 33 prepared prepare VBN cord-283998-whwksoxt 136 34 by by IN cord-283998-whwksoxt 136 35 SDS SDS NNP cord-283998-whwksoxt 136 36 lysis lysis NN cord-283998-whwksoxt 136 37 . . . cord-283998-whwksoxt 137 1 The the DT cord-283998-whwksoxt 137 2 profile profile NN cord-283998-whwksoxt 137 3 for for IN cord-283998-whwksoxt 137 4 total total JJ cord-283998-whwksoxt 137 5 radioactivity radioactivity NN cord-283998-whwksoxt 137 6 ( ( -LRB- cord-283998-whwksoxt 137 7 Fig Fig NNP cord-283998-whwksoxt 137 8 . . . cord-283998-whwksoxt 137 9 7C 7C NNP cord-283998-whwksoxt 137 10 ) ) -RRB- cord-283998-whwksoxt 137 11 shows show VBZ cord-283998-whwksoxt 137 12 a a DT cord-283998-whwksoxt 137 13 complete complete JJ cord-283998-whwksoxt 137 14 breakdown breakdown NN cord-283998-whwksoxt 137 15 of of IN cord-283998-whwksoxt 137 16 the the DT cord-283998-whwksoxt 137 17 70 70 CD cord-283998-whwksoxt 137 18 S S NNP cord-283998-whwksoxt 137 19 RNA RNA NNP cord-283998-whwksoxt 137 20 complex complex NN cord-283998-whwksoxt 137 21 to to IN cord-283998-whwksoxt 137 22 smaller small JJR cord-283998-whwksoxt 137 23 fragments fragment NNS cord-283998-whwksoxt 137 24 which which WDT cord-283998-whwksoxt 137 25 sediment sediment VBP cord-283998-whwksoxt 137 26 slightly slightly RB cord-283998-whwksoxt 137 27 faster fast RBR cord-283998-whwksoxt 137 28 than than IN cord-283998-whwksoxt 137 29 structural structural JJ cord-283998-whwksoxt 137 30 phospholipids phospholipid NNS cord-283998-whwksoxt 137 31 at at IN cord-283998-whwksoxt 137 32 the the DT cord-283998-whwksoxt 137 33 top top NN cord-283998-whwksoxt 137 34 of of IN cord-283998-whwksoxt 137 35 the the DT cord-283998-whwksoxt 137 36 gradient gradient NN cord-283998-whwksoxt 137 37 ( ( -LRB- cord-283998-whwksoxt 137 38 Fig Fig NNP cord-283998-whwksoxt 137 39 . . . cord-283998-whwksoxt 137 40 7A 7a NN cord-283998-whwksoxt 137 41 ) ) -RRB- cord-283998-whwksoxt 137 42 . . . cord-283998-whwksoxt 138 1 Duesberg Duesberg NNP cord-283998-whwksoxt 138 2 ( ( -LRB- cord-283998-whwksoxt 138 3 1968 1968 CD cord-283998-whwksoxt 138 4 ) ) -RRB- cord-283998-whwksoxt 138 5 noted note VBD cord-283998-whwksoxt 138 6 similar similar JJ cord-283998-whwksoxt 138 7 instability instability NN cord-283998-whwksoxt 138 8 for for IN cord-283998-whwksoxt 138 9 RSV RSV NNP cord-283998-whwksoxt 138 10 70 70 NNP cord-283998-whwksoxt 138 11 S S NNP cord-283998-whwksoxt 138 12 RNA RNA NNP cord-283998-whwksoxt 138 13 , , , cord-283998-whwksoxt 138 14 whose whose WP$ cord-283998-whwksoxt 138 15 constituent constituent NN cord-283998-whwksoxt 138 16 RNA RNA NNP cord-283998-whwksoxt 138 17 fragments fragment NNS cord-283998-whwksoxt 138 18 are be VBP cord-283998-whwksoxt 138 19 held hold VBN cord-283998-whwksoxt 138 20 together together RB cord-283998-whwksoxt 138 21 by by IN cord-283998-whwksoxt 138 22 noncovalent noncovalent NN cord-283998-whwksoxt 138 23 bonds bond NNS cord-283998-whwksoxt 138 24 less less RBR cord-283998-whwksoxt 138 25 susceptible susceptible JJ cord-283998-whwksoxt 138 26 to to IN cord-283998-whwksoxt 138 27 phenol phenol NN cord-283998-whwksoxt 138 28 extraction extraction NN cord-283998-whwksoxt 138 29 than than IN cord-283998-whwksoxt 138 30 OC-43 OC-43 NNP cord-283998-whwksoxt 138 31 RNA RNA NNP cord-283998-whwksoxt 138 32 ( ( -LRB- cord-283998-whwksoxt 138 33 Fig Fig NNP cord-283998-whwksoxt 138 34 . . . cord-283998-whwksoxt 138 35 2B 2b CD cord-283998-whwksoxt 138 36 ) ) -RRB- cord-283998-whwksoxt 138 37 . . . cord-283998-whwksoxt 139 1 The the DT cord-283998-whwksoxt 139 2 OC-43 OC-43 NNP cord-283998-whwksoxt 139 3 RNA RNA NNP cord-283998-whwksoxt 139 4 complex complex NN cord-283998-whwksoxt 139 5 released release VBN cord-283998-whwksoxt 139 6 by by IN cord-283998-whwksoxt 139 7 SDS SDS NNP cord-283998-whwksoxt 139 8 lysis lysis NN cord-283998-whwksoxt 139 9 and and CC cord-283998-whwksoxt 139 10 70 70 CD cord-283998-whwksoxt 139 11 S S NNP cord-283998-whwksoxt 139 12 RSV RSV NNP cord-283998-whwksoxt 139 13 RNA RNA NNP cord-283998-whwksoxt 139 14 have have VBP cord-283998-whwksoxt 139 15 identical identical JJ cord-283998-whwksoxt 139 16 sedimentation sedimentation NN cord-283998-whwksoxt 139 17 coefficients coefficient NNS cord-283998-whwksoxt 139 18 . . . cord-283998-whwksoxt 140 1 The the DT cord-283998-whwksoxt 140 2 latter latter JJ cord-283998-whwksoxt 140 3 is be VBZ cord-283998-whwksoxt 140 4 a a DT cord-283998-whwksoxt 140 5 complex complex NN cord-283998-whwksoxt 140 6 of of IN cord-283998-whwksoxt 140 7 several several JJ cord-283998-whwksoxt 140 8 noncovalently noncovalently RB cord-283998-whwksoxt 140 9 linked link VBN cord-283998-whwksoxt 140 10 subunits subunit NNS cord-283998-whwksoxt 140 11 ( ( -LRB- cord-283998-whwksoxt 140 12 MW MW NNP cord-283998-whwksoxt 140 13 2.5 2.5 CD cord-283998-whwksoxt 140 14 - - SYM cord-283998-whwksoxt 140 15 3.3 3.3 CD cord-283998-whwksoxt 140 16 x x SYM cord-283998-whwksoxt 140 17 lo lo NNP cord-283998-whwksoxt 140 18 ' ' '' cord-283998-whwksoxt 140 19 ? ? . cord-283998-whwksoxt 141 1 which which WDT cord-283998-whwksoxt 141 2 are be VBP cord-283998-whwksoxt 141 3 freed free VBN cord-283998-whwksoxt 141 4 by by IN cord-283998-whwksoxt 141 5 heating heating NN cord-283998-whwksoxt 141 6 or or CC cord-283998-whwksoxt 141 7 treatment treatment NN cord-283998-whwksoxt 141 8 with with IN cord-283998-whwksoxt 141 9 DMSO DMSO NNP cord-283998-whwksoxt 141 10 ( ( -LRB- cord-283998-whwksoxt 141 11 Duesberg Duesberg NNP cord-283998-whwksoxt 141 12 , , , cord-283998-whwksoxt 141 13 1968 1968 CD cord-283998-whwksoxt 141 14 ; ; : cord-283998-whwksoxt 141 15 King King NNP cord-283998-whwksoxt 141 16 , , , cord-283998-whwksoxt 141 17 1976 1976 CD cord-283998-whwksoxt 141 18 ) ) -RRB- cord-283998-whwksoxt 141 19 . . . cord-283998-whwksoxt 142 1 In in IN cord-283998-whwksoxt 142 2 addition addition NN cord-283998-whwksoxt 142 3 to to IN cord-283998-whwksoxt 142 4 70 70 CD cord-283998-whwksoxt 142 5 S S NNP cord-283998-whwksoxt 142 6 RNA RNA NNP cord-283998-whwksoxt 142 7 , , , cord-283998-whwksoxt 142 8 a a DT cord-283998-whwksoxt 142 9 range range NN cord-283998-whwksoxt 142 10 of of IN cord-283998-whwksoxt 142 11 smaller small JJR cord-283998-whwksoxt 142 12 fragments fragment NNS cord-283998-whwksoxt 142 13 , , , cord-283998-whwksoxt 142 14 including include VBG cord-283998-whwksoxt 142 15 a a DT cord-283998-whwksoxt 142 16 4 4 CD cord-283998-whwksoxt 142 17 S S NNP cord-283998-whwksoxt 142 18 species specie NNS cord-283998-whwksoxt 142 19 , , , cord-283998-whwksoxt 142 20 is be VBZ cord-283998-whwksoxt 142 21 present present JJ cord-283998-whwksoxt 142 22 in in IN cord-283998-whwksoxt 142 23 phenol phenol NN cord-283998-whwksoxt 142 24 extracts extract NNS cord-283998-whwksoxt 142 25 of of IN cord-283998-whwksoxt 142 26 RSV RSV NNP cord-283998-whwksoxt 143 1 ( ( -LRB- cord-283998-whwksoxt 143 2 Robinson Robinson NNP cord-283998-whwksoxt 143 3 et et NNP cord-283998-whwksoxt 143 4 al al NNP cord-283998-whwksoxt 143 5 . . NNP cord-283998-whwksoxt 143 6 , , , cord-283998-whwksoxt 143 7 1965 1965 CD cord-283998-whwksoxt 143 8 ; ; : cord-283998-whwksoxt 143 9 Duesberg Duesberg NNP cord-283998-whwksoxt 143 10 , , , cord-283998-whwksoxt 143 11 1968 1968 CD cord-283998-whwksoxt 143 12 ; ; : cord-283998-whwksoxt 143 13 Bishop Bishop NNP cord-283998-whwksoxt 143 14 et et NNP cord-283998-whwksoxt 143 15 al al NNP cord-283998-whwksoxt 143 16 . . NNP cord-283998-whwksoxt 143 17 , , , cord-283998-whwksoxt 143 18 1970a 1970a CD cord-283998-whwksoxt 143 19 , , , cord-283998-whwksoxt 143 20 b b LS cord-283998-whwksoxt 143 21 ; ; : cord-283998-whwksoxt 143 22 Dahlberg Dahlberg NNP cord-283998-whwksoxt 143 23 et et FW cord-283998-whwksoxt 143 24 al al NNP cord-283998-whwksoxt 143 25 . . NNP cord-283998-whwksoxt 143 26 , , , cord-283998-whwksoxt 143 27 1974 1974 CD cord-283998-whwksoxt 143 28 ) ) -RRB- cord-283998-whwksoxt 143 29 . . . cord-283998-whwksoxt 144 1 The the DT cord-283998-whwksoxt 144 2 70 70 CD cord-283998-whwksoxt 144 3 S S NNP cord-283998-whwksoxt 144 4 OC-43 OC-43 NNP cord-283998-whwksoxt 144 5 complex complex NN cord-283998-whwksoxt 144 6 may may MD cord-283998-whwksoxt 144 7 also also RB cord-283998-whwksoxt 144 8 consist consist VB cord-283998-whwksoxt 144 9 of of IN cord-283998-whwksoxt 144 10 noncovalently noncovalently RB cord-283998-whwksoxt 144 11 linked link VBN cord-283998-whwksoxt 144 12 RNA RNA NNP cord-283998-whwksoxt 144 13 subunits subunit NNS cord-283998-whwksoxt 144 14 which which WDT cord-283998-whwksoxt 144 15 are be VBP cord-283998-whwksoxt 144 16 disrupted disrupt VBN cord-283998-whwksoxt 144 17 by by IN cord-283998-whwksoxt 144 18 DMSO DMSO NNP cord-283998-whwksoxt 144 19 , , , cord-283998-whwksoxt 144 20 but but CC cord-283998-whwksoxt 144 21 unlike unlike IN cord-283998-whwksoxt 144 22 70 70 CD cord-283998-whwksoxt 144 23 S s NN cord-283998-whwksoxt 144 24 RSV RSV NNP cord-283998-whwksoxt 144 25 , , , cord-283998-whwksoxt 144 26 are be VBP cord-283998-whwksoxt 144 27 highly highly RB cord-283998-whwksoxt 144 28 susceptible susceptible JJ cord-283998-whwksoxt 144 29 to to IN cord-283998-whwksoxt 144 30 phenol phenol NN cord-283998-whwksoxt 144 31 . . . cord-283998-whwksoxt 145 1 The the DT cord-283998-whwksoxt 145 2 complex complex NN cord-283998-whwksoxt 145 3 is be VBZ cord-283998-whwksoxt 145 4 more more RBR cord-283998-whwksoxt 145 5 readily readily RB cord-283998-whwksoxt 145 6 broken break VBN cord-283998-whwksoxt 145 7 down down RP cord-283998-whwksoxt 145 8 by by IN cord-283998-whwksoxt 145 9 heating heating NN cord-283998-whwksoxt 145 10 than than IN cord-283998-whwksoxt 145 11 are be VBP cord-283998-whwksoxt 145 12 similar similar JJ cord-283998-whwksoxt 145 13 complexes complex NNS cord-283998-whwksoxt 145 14 isolated isolate VBN cord-283998-whwksoxt 145 15 from from IN cord-283998-whwksoxt 145 16 RSV RSV NNP cord-283998-whwksoxt 145 17 ( ( -LRB- cord-283998-whwksoxt 145 18 Duesberg Duesberg NNP cord-283998-whwksoxt 145 19 , , , cord-283998-whwksoxt 145 20 1968 1968 CD cord-283998-whwksoxt 145 21 ) ) -RRB- cord-283998-whwksoxt 145 22 and and CC cord-283998-whwksoxt 145 23 the the DT cord-283998-whwksoxt 145 24 TGEV TGEV NNS cord-283998-whwksoxt 145 25 and and CC cord-283998-whwksoxt 145 26 HEV HEV NNP cord-283998-whwksoxt 145 27 coronaviruses coronaviruse NNS cord-283998-whwksoxt 145 28 . . . cord-283998-whwksoxt 146 1 Its -PRON- PRP$ cord-283998-whwksoxt 146 2 apparent apparent JJ cord-283998-whwksoxt 146 3 molecular molecular JJ cord-283998-whwksoxt 146 4 weight weight NN cord-283998-whwksoxt 146 5 , , , cord-283998-whwksoxt 146 6 determined determine VBD cord-283998-whwksoxt 146 7 comparatively comparatively RB cord-283998-whwksoxt 146 8 by by IN cord-283998-whwksoxt 146 9 coelectrophoresis coelectrophoresis NN cord-283998-whwksoxt 146 10 with with IN cord-283998-whwksoxt 146 11 other other JJ cord-283998-whwksoxt 146 12 RNAs rna NNS cord-283998-whwksoxt 146 13 , , , cord-283998-whwksoxt 146 14 is be VBZ cord-283998-whwksoxt 146 15 6.1 6.1 CD cord-283998-whwksoxt 146 16 x x SYM cord-283998-whwksoxt 146 17 106 106 CD cord-283998-whwksoxt 146 18 ; ; : cord-283998-whwksoxt 146 19 this this DT cord-283998-whwksoxt 146 20 value value NN cord-283998-whwksoxt 146 21 is be VBZ cord-283998-whwksoxt 146 22 only only RB cord-283998-whwksoxt 146 23 considered consider VBN cord-283998-whwksoxt 146 24 to to TO cord-283998-whwksoxt 146 25 be be VB cord-283998-whwksoxt 146 26 approximate approximate JJ cord-283998-whwksoxt 146 27 , , , cord-283998-whwksoxt 146 28 however however RB cord-283998-whwksoxt 146 29 , , , cord-283998-whwksoxt 146 30 since since IN cord-283998-whwksoxt 146 31 2.2 2.2 CD cord-283998-whwksoxt 146 32 % % NN cord-283998-whwksoxt 146 33 gels gel NNS cord-283998-whwksoxt 146 34 do do VBP cord-283998-whwksoxt 146 35 not not RB cord-283998-whwksoxt 146 36 adequately adequately RB cord-283998-whwksoxt 146 37 resolve resolve VB cord-283998-whwksoxt 146 38 high high JJ cord-283998-whwksoxt 146 39 molecular molecular JJ cord-283998-whwksoxt 146 40 - - HYPH cord-283998-whwksoxt 146 41 weight weight NN cord-283998-whwksoxt 146 42 RNAs rna NNS cord-283998-whwksoxt 146 43 ( ( -LRB- cord-283998-whwksoxt 146 44 Loening Loening NNP cord-283998-whwksoxt 146 45 , , , cord-283998-whwksoxt 146 46 1969 1969 CD cord-283998-whwksoxt 146 47 ; ; : cord-283998-whwksoxt 146 48 King King NNP cord-283998-whwksoxt 146 49 , , , cord-283998-whwksoxt 146 50 1976 1976 CD cord-283998-whwksoxt 146 51 ) ) -RRB- cord-283998-whwksoxt 146 52 . . . cord-283998-whwksoxt 147 1 Native native JJ cord-283998-whwksoxt 147 2 OC-43 OC-43 NNP cord-283998-whwksoxt 147 3 RNA RNA NNP cord-283998-whwksoxt 147 4 is be VBZ cord-283998-whwksoxt 147 5 readily readily RB cord-283998-whwksoxt 147 6 denatured denature VBN cord-283998-whwksoxt 147 7 into into IN cord-283998-whwksoxt 147 8 fragments fragment NNS cord-283998-whwksoxt 147 9 by by IN cord-283998-whwksoxt 147 10 organic organic JJ cord-283998-whwksoxt 147 11 solvents solvent NNS cord-283998-whwksoxt 147 12 and and CC cord-283998-whwksoxt 147 13 heat heat NN cord-283998-whwksoxt 147 14 , , , cord-283998-whwksoxt 147 15 much much RB cord-283998-whwksoxt 147 16 like like IN cord-283998-whwksoxt 147 17 that that DT cord-283998-whwksoxt 147 18 observed observe VBN cord-283998-whwksoxt 147 19 with with IN cord-283998-whwksoxt 147 20 B77 B77 NNP cord-283998-whwksoxt 147 21 sarcoma sarcoma NN cord-283998-whwksoxt 147 22 virus virus NN cord-283998-whwksoxt 147 23 RNA RNA NNP cord-283998-whwksoxt 147 24 by by IN cord-283998-whwksoxt 147 25 Stoltzfus Stoltzfus NNP cord-283998-whwksoxt 147 26 and and CC cord-283998-whwksoxt 147 27 Snyder Snyder NNP cord-283998-whwksoxt 147 28 ( ( -LRB- cord-283998-whwksoxt 147 29 1975 1975 CD cord-283998-whwksoxt 147 30 ) ) -RRB- cord-283998-whwksoxt 147 31 . . . cord-283998-whwksoxt 148 1 However however RB cord-283998-whwksoxt 148 2 , , , cord-283998-whwksoxt 148 3 the the DT cord-283998-whwksoxt 148 4 sizes size NNS cord-283998-whwksoxt 148 5 of of IN cord-283998-whwksoxt 148 6 the the DT cord-283998-whwksoxt 148 7 OC-43 OC-43 NNP cord-283998-whwksoxt 148 8 RNA RNA NNP cord-283998-whwksoxt 148 9 fragments fragment NNS cord-283998-whwksoxt 148 10 from from IN cord-283998-whwksoxt 148 11 the the DT cord-283998-whwksoxt 148 12 disrupted disrupt VBN cord-283998-whwksoxt 148 13 complex complex NN cord-283998-whwksoxt 148 14 seem seem VBP cord-283998-whwksoxt 148 15 to to TO cord-283998-whwksoxt 148 16 vary vary VB cord-283998-whwksoxt 148 17 considerably considerably RB cord-283998-whwksoxt 148 18 . . . cord-283998-whwksoxt 149 1 Phenol phenol NN cord-283998-whwksoxt 149 2 extraction extraction NN cord-283998-whwksoxt 149 3 of of IN cord-283998-whwksoxt 149 4 purified purify VBN cord-283998-whwksoxt 149 5 virions virion NNS cord-283998-whwksoxt 149 6 produces produce VBZ cord-283998-whwksoxt 149 7 fragments fragment NNS cord-283998-whwksoxt 149 8 ranging range VBG cord-283998-whwksoxt 149 9 between between IN cord-283998-whwksoxt 149 10 15 15 CD cord-283998-whwksoxt 149 11 and and CC cord-283998-whwksoxt 149 12 50 50 CD cord-283998-whwksoxt 149 13 S S NNS cord-283998-whwksoxt 149 14 , , , cord-283998-whwksoxt 149 15 and and CC cord-283998-whwksoxt 149 16 a a DT cord-283998-whwksoxt 149 17 small small JJ cord-283998-whwksoxt 149 18 amount amount NN cord-283998-whwksoxt 149 19 of of IN cord-283998-whwksoxt 149 20 a a DT cord-283998-whwksoxt 149 21 homogeneous homogeneous JJ cord-283998-whwksoxt 149 22 4 4 CD cord-283998-whwksoxt 149 23 S S NNP cord-283998-whwksoxt 149 24 RNA RNA NNP cord-283998-whwksoxt 149 25 is be VBZ cord-283998-whwksoxt 149 26 also also RB cord-283998-whwksoxt 149 27 present present JJ cord-283998-whwksoxt 149 28 ( ( -LRB- cord-283998-whwksoxt 149 29 Figs fig NNS cord-283998-whwksoxt 149 30 . . . cord-283998-whwksoxt 149 31 2 2 CD cord-283998-whwksoxt 149 32 , , , cord-283998-whwksoxt 149 33 3 3 CD cord-283998-whwksoxt 149 34 , , , cord-283998-whwksoxt 149 35 6 6 CD cord-283998-whwksoxt 149 36 ) ) -RRB- cord-283998-whwksoxt 149 37 . . . cord-283998-whwksoxt 150 1 A a DT cord-283998-whwksoxt 150 2 range range NN cord-283998-whwksoxt 150 3 of of IN cord-283998-whwksoxt 150 4 fragments fragment NNS cord-283998-whwksoxt 150 5 of of IN cord-283998-whwksoxt 150 6 molecular molecular JJ cord-283998-whwksoxt 150 7 weight weight NN cord-283998-whwksoxt 150 8 0.7 0.7 CD cord-283998-whwksoxt 150 9 - - HYPH cord-283998-whwksoxt 150 10 5 5 CD cord-283998-whwksoxt 151 1 x x LS cord-283998-whwksoxt 151 2 lo6 lo6 NNP cord-283998-whwksoxt 151 3 and and CC cord-283998-whwksoxt 151 4 a a DT cord-283998-whwksoxt 151 5 smaller small JJR cord-283998-whwksoxt 151 6 species specie NNS cord-283998-whwksoxt 151 7 was be VBD cord-283998-whwksoxt 151 8 obtained obtain VBN cord-283998-whwksoxt 151 9 after after IN cord-283998-whwksoxt 151 10 PAGE page NN cord-283998-whwksoxt 151 11 , , , cord-283998-whwksoxt 151 12 similar similar JJ cord-283998-whwksoxt 151 13 to to IN cord-283998-whwksoxt 151 14 that that DT cord-283998-whwksoxt 151 15 described describe VBN cord-283998-whwksoxt 151 16 by by IN cord-283998-whwksoxt 151 17 Tanneck Tanneck NNP cord-283998-whwksoxt 151 18 ( ( -LRB- cord-283998-whwksoxt 151 19 1973 1973 CD cord-283998-whwksoxt 151 20 ) ) -RRB- cord-283998-whwksoxt 151 21 for for IN cord-283998-whwksoxt 151 22 IBV IBV NNP cord-283998-whwksoxt 151 23 RNA RNA NNP cord-283998-whwksoxt 151 24 . . . cord-283998-whwksoxt 152 1 Coextraction Coextraction NNP cord-283998-whwksoxt 152 2 experiments experiment NNS cord-283998-whwksoxt 152 3 with with IN cord-283998-whwksoxt 152 4 TMV TMV NNP cord-283998-whwksoxt 152 5 and and CC cord-283998-whwksoxt 152 6 RSV RSV NNP cord-283998-whwksoxt 153 1 ( ( -LRB- cord-283998-whwksoxt 153 2 Fig Fig NNP cord-283998-whwksoxt 153 3 . . . cord-283998-whwksoxt 154 1 6 6 LS cord-283998-whwksoxt 154 2 ) ) -RRB- cord-283998-whwksoxt 154 3 did do VBD cord-283998-whwksoxt 154 4 not not RB cord-283998-whwksoxt 154 5 implicate implicate VB cord-283998-whwksoxt 154 6 internal internal JJ cord-283998-whwksoxt 154 7 ribonuclease ribonuclease NN cord-283998-whwksoxt 154 8 activity activity NN cord-283998-whwksoxt 154 9 as as IN cord-283998-whwksoxt 154 10 a a DT cord-283998-whwksoxt 154 11 cause cause NN cord-283998-whwksoxt 154 12 of of IN cord-283998-whwksoxt 154 13 this this DT cord-283998-whwksoxt 154 14 heterogeneity heterogeneity NN cord-283998-whwksoxt 154 15 . . . cord-283998-whwksoxt 155 1 When when WRB cord-283998-whwksoxt 155 2 the the DT cord-283998-whwksoxt 155 3 70 70 CD cord-283998-whwksoxt 155 4 S S NNP cord-283998-whwksoxt 155 5 complex complex NN cord-283998-whwksoxt 155 6 released release VBN cord-283998-whwksoxt 155 7 by by IN cord-283998-whwksoxt 155 8 SDS SDS NNP cord-283998-whwksoxt 155 9 lysis lysis NN cord-283998-whwksoxt 155 10 was be VBD cord-283998-whwksoxt 155 11 isolated isolate VBN cord-283998-whwksoxt 155 12 and and CC cord-283998-whwksoxt 155 13 reextracted reextracte VBN cord-283998-whwksoxt 155 14 with with IN cord-283998-whwksoxt 155 15 phenolchloroform phenolchloroform NN cord-283998-whwksoxt 155 16 , , , cord-283998-whwksoxt 155 17 only only RB cord-283998-whwksoxt 155 18 5 5 CD cord-283998-whwksoxt 155 19 - - SYM cord-283998-whwksoxt 155 20 10 10 CD cord-283998-whwksoxt 155 21 S S NNP cord-283998-whwksoxt 155 22 RNA RNA NNP cord-283998-whwksoxt 155 23 fragments fragment NNS cord-283998-whwksoxt 155 24 were be VBD cord-283998-whwksoxt 155 25 obtained obtain VBN cord-283998-whwksoxt 155 26 ( ( -LRB- cord-283998-whwksoxt 155 27 Fig Fig NNP cord-283998-whwksoxt 155 28 . . NNP cord-283998-whwksoxt 155 29 7 7 CD cord-283998-whwksoxt 155 30 ) ) -RRB- cord-283998-whwksoxt 155 31 . . . cord-283998-whwksoxt 156 1 Why why WRB cord-283998-whwksoxt 156 2 fragments fragment NNS cord-283998-whwksoxt 156 3 extracted extract VBN cord-283998-whwksoxt 156 4 in in IN cord-283998-whwksoxt 156 5 this this DT cord-283998-whwksoxt 156 6 manner manner NN cord-283998-whwksoxt 156 7 should should MD cord-283998-whwksoxt 156 8 be be VB cord-283998-whwksoxt 156 9 smaller small JJR cord-283998-whwksoxt 156 10 is be VBZ cord-283998-whwksoxt 156 11 unknown unknown JJ cord-283998-whwksoxt 156 12 . . . cord-283998-whwksoxt 157 1 Again again RB cord-283998-whwksoxt 157 2 , , , cord-283998-whwksoxt 157 3 however however RB cord-283998-whwksoxt 157 4 , , , cord-283998-whwksoxt 157 5 ribonucleases ribonuclease NNS cord-283998-whwksoxt 157 6 could could MD cord-283998-whwksoxt 157 7 not not RB cord-283998-whwksoxt 157 8 be be VB cord-283998-whwksoxt 157 9 detected detect VBN cord-283998-whwksoxt 157 10 , , , cord-283998-whwksoxt 157 11 because because IN cord-283998-whwksoxt 157 12 a a DT cord-283998-whwksoxt 157 13 preparation preparation NN cord-283998-whwksoxt 157 14 of of IN cord-283998-whwksoxt 157 15 TMV TMV NNP cord-283998-whwksoxt 157 16 RNA RNA NNP cord-283998-whwksoxt 157 17 , , , cord-283998-whwksoxt 157 18 when when WRB cord-283998-whwksoxt 157 19 included include VBN cord-283998-whwksoxt 157 20 in in IN cord-283998-whwksoxt 157 21 the the DT cord-283998-whwksoxt 157 22 extraction extraction NN cord-283998-whwksoxt 157 23 mixture mixture NN cord-283998-whwksoxt 157 24 , , , cord-283998-whwksoxt 157 25 remained remain VBD cord-283998-whwksoxt 157 26 undegraded undegraded JJ cord-283998-whwksoxt 157 27 ( ( -LRB- cord-283998-whwksoxt 157 28 Fig Fig NNP cord-283998-whwksoxt 157 29 . . . cord-283998-whwksoxt 157 30 7B 7B NNP cord-283998-whwksoxt 157 31 ) ) -RRB- cord-283998-whwksoxt 157 32 . . . cord-283998-whwksoxt 158 1 Possibly possibly RB cord-283998-whwksoxt 158 2 , , , cord-283998-whwksoxt 158 3 RNA RNA NNP cord-283998-whwksoxt 158 4 extracted extract VBN cord-283998-whwksoxt 158 5 by by IN cord-283998-whwksoxt 158 6 phenol phenol NN cord-283998-whwksoxt 158 7 from from IN cord-283998-whwksoxt 158 8 purified purify VBN cord-283998-whwksoxt 158 9 virions virion NNS cord-283998-whwksoxt 158 10 contains contain VBZ cord-283998-whwksoxt 158 11 more more JJR cord-283998-whwksoxt 158 12 secondary secondary JJ cord-283998-whwksoxt 158 13 structure structure NN cord-283998-whwksoxt 158 14 . . . cord-283998-whwksoxt 159 1 Alternatively alternatively RB cord-283998-whwksoxt 159 2 ribonucleases ribonucleases NNP cord-283998-whwksoxt 159 3 could could MD cord-283998-whwksoxt 159 4 have have VB cord-283998-whwksoxt 159 5 been be VBN cord-283998-whwksoxt 159 6 present present JJ cord-283998-whwksoxt 159 7 throughout throughout IN cord-283998-whwksoxt 159 8 viral viral JJ cord-283998-whwksoxt 159 9 growth growth NN cord-283998-whwksoxt 159 10 , , , cord-283998-whwksoxt 159 11 producing produce VBG cord-283998-whwksoxt 159 12 nicks nick NNS cord-283998-whwksoxt 159 13 in in IN cord-283998-whwksoxt 159 14 an an DT cord-283998-whwksoxt 159 15 otherwise otherwise RB cord-283998-whwksoxt 159 16 intact intact JJ cord-283998-whwksoxt 159 17 RNA rna NN cord-283998-whwksoxt 159 18 molecule molecule NN cord-283998-whwksoxt 159 19 and and CC cord-283998-whwksoxt 159 20 subsequently subsequently RB cord-283998-whwksoxt 159 21 being be VBG cord-283998-whwksoxt 159 22 destroyed destroy VBN cord-283998-whwksoxt 159 23 by by IN cord-283998-whwksoxt 159 24 phenol phenol NN cord-283998-whwksoxt 159 25 - - HYPH cord-283998-whwksoxt 159 26 chloroform chloroform NN cord-283998-whwksoxt 159 27 extraction extraction NN cord-283998-whwksoxt 159 28 . . . cord-283998-whwksoxt 160 1 The the DT cord-283998-whwksoxt 160 2 release release NN cord-283998-whwksoxt 160 3 of of IN cord-283998-whwksoxt 160 4 4 4 CD cord-283998-whwksoxt 160 5 S S NNP cord-283998-whwksoxt 160 6 RNA RNA NNP cord-283998-whwksoxt 160 7 from from IN cord-283998-whwksoxt 160 8 the the DT cord-283998-whwksoxt 160 9 70 70 CD cord-283998-whwksoxt 160 10 S S NNP cord-283998-whwksoxt 160 11 complex complex NN cord-283998-whwksoxt 160 12 by by IN cord-283998-whwksoxt 160 13 heating heating NN cord-283998-whwksoxt 160 14 has have VBZ cord-283998-whwksoxt 160 15 also also RB cord-283998-whwksoxt 160 16 been be VBN cord-283998-whwksoxt 160 17 observed observe VBN cord-283998-whwksoxt 160 18 for for IN cord-283998-whwksoxt 160 19 TGEV TGEV NNP cord-283998-whwksoxt 160 20 and and CC cord-283998-whwksoxt 160 21 HEV HEV NNP cord-283998-whwksoxt 160 22 RNAs rna NNS cord-283998-whwksoxt 160 23 . . . cord-283998-whwksoxt 161 1 Recent recent JJ cord-283998-whwksoxt 161 2 studies study NNS cord-283998-whwksoxt 161 3 have have VBP cord-283998-whwksoxt 161 4 shown show VBN cord-283998-whwksoxt 161 5 that that IN cord-283998-whwksoxt 161 6 polyadenylate polyadenylate NN cord-283998-whwksoxt 161 7 residues residue NNS cord-283998-whwksoxt 161 8 are be VBP cord-283998-whwksoxt 161 9 present present JJ cord-283998-whwksoxt 161 10 in in IN cord-283998-whwksoxt 161 11 15 15 CD cord-283998-whwksoxt 161 12 - - SYM cord-283998-whwksoxt 161 13 55 55 CD cord-283998-whwksoxt 161 14 S s NN cord-283998-whwksoxt 162 1 but but CC cord-283998-whwksoxt 162 2 not not RB cord-283998-whwksoxt 162 3 in in IN cord-283998-whwksoxt 162 4 the the DT cord-283998-whwksoxt 162 5 4 4 CD cord-283998-whwksoxt 162 6 S S NNP cord-283998-whwksoxt 162 7 RNAs rna NNS cord-283998-whwksoxt 162 8 obtained obtain VBN cord-283998-whwksoxt 162 9 by by IN cord-283998-whwksoxt 162 10 PC pc NN cord-283998-whwksoxt 162 11 extraction extraction NN cord-283998-whwksoxt 162 12 ( ( -LRB- cord-283998-whwksoxt 162 13 Tannock Tannock NNP cord-283998-whwksoxt 162 14 and and CC cord-283998-whwksoxt 162 15 Hierholzer Hierholzer NNP cord-283998-whwksoxt 162 16 , , , cord-283998-whwksoxt 163 1 in in IN cord-283998-whwksoxt 163 2 preparation preparation NN cord-283998-whwksoxt 163 3 ) ) -RRB- cord-283998-whwksoxt 163 4 , , , cord-283998-whwksoxt 163 5 indicating indicate VBG cord-283998-whwksoxt 163 6 a a DT cord-283998-whwksoxt 163 7 functional functional JJ cord-283998-whwksoxt 163 8 difference difference NN cord-283998-whwksoxt 163 9 between between IN cord-283998-whwksoxt 163 10 these these DT cord-283998-whwksoxt 163 11 RNA RNA NNP cord-283998-whwksoxt 163 12 classes class NNS cord-283998-whwksoxt 163 13 . . . cord-283998-whwksoxt 164 1 Detailed detailed JJ cord-283998-whwksoxt 164 2 studies study NNS cord-283998-whwksoxt 164 3 are be VBP cord-283998-whwksoxt 164 4 needed need VBN cord-283998-whwksoxt 164 5 to to TO cord-283998-whwksoxt 164 6 determine determine VB cord-283998-whwksoxt 164 7 what what WP cord-283998-whwksoxt 164 8 these these DT cord-283998-whwksoxt 164 9 functions function NNS cord-283998-whwksoxt 164 10 are be VBP cord-283998-whwksoxt 164 11 . . . cord-283998-whwksoxt 165 1 Although although IN cord-283998-whwksoxt 165 2 SDS SDS NNP cord-283998-whwksoxt 165 3 is be VBZ cord-283998-whwksoxt 165 4 recognized recognize VBN cord-283998-whwksoxt 165 5 as as IN cord-283998-whwksoxt 165 6 an an DT cord-283998-whwksoxt 165 7 effective effective JJ cord-283998-whwksoxt 165 8 RNA RNA NNP cord-283998-whwksoxt 165 9 deproteinizing deproteinizing NN cord-283998-whwksoxt 165 10 agent agent NN cord-283998-whwksoxt 165 11 ( ( -LRB- cord-283998-whwksoxt 165 12 Boatman Boatman NNP cord-283998-whwksoxt 165 13 and and CC cord-283998-whwksoxt 165 14 Kaper Kaper NNP cord-283998-whwksoxt 165 15 , , , cord-283998-whwksoxt 165 16 1973 1973 CD cord-283998-whwksoxt 165 17 ) ) -RRB- cord-283998-whwksoxt 165 18 , , , cord-283998-whwksoxt 165 19 the the DT cord-283998-whwksoxt 165 20 susceptibility susceptibility NN cord-283998-whwksoxt 165 21 of of IN cord-283998-whwksoxt 165 22 the the DT cord-283998-whwksoxt 165 23 70 70 CD cord-283998-whwksoxt 165 24 S S NNP cord-283998-whwksoxt 165 25 OC-43 OC-43 NNP cord-283998-whwksoxt 165 26 complex complex JJ cord-283998-whwksoxt 165 27 to to IN cord-283998-whwksoxt 165 28 phenol phenol NN cord-283998-whwksoxt 165 29 suggests suggest VBZ cord-283998-whwksoxt 165 30 that that IN cord-283998-whwksoxt 165 31 small small JJ cord-283998-whwksoxt 165 32 protein protein NN cord-283998-whwksoxt 165 33 linkers linker NNS cord-283998-whwksoxt 165 34 could could MD cord-283998-whwksoxt 165 35 be be VB cord-283998-whwksoxt 165 36 required require VBN cord-283998-whwksoxt 165 37 to to TO cord-283998-whwksoxt 165 38 hold hold VB cord-283998-whwksoxt 165 39 the the DT cord-283998-whwksoxt 165 40 RNA rna NN cord-283998-whwksoxt 165 41 fragments fragment NNS cord-283998-whwksoxt 165 42 together together RB cord-283998-whwksoxt 165 43 and and CC cord-283998-whwksoxt 165 44 that that IN cord-283998-whwksoxt 165 45 they -PRON- PRP cord-283998-whwksoxt 165 46 are be VBP cord-283998-whwksoxt 165 47 not not RB cord-283998-whwksoxt 165 48 removable removable JJ cord-283998-whwksoxt 165 49 by by IN cord-283998-whwksoxt 165 50 SDS sds NN cord-283998-whwksoxt 165 51 lysis lysis NN cord-283998-whwksoxt 165 52 . . . cord-283998-whwksoxt 166 1 Evidence evidence NN cord-283998-whwksoxt 166 2 against against IN cord-283998-whwksoxt 166 3 this this DT cord-283998-whwksoxt 166 4 comes come VBZ cord-283998-whwksoxt 166 5 from from IN cord-283998-whwksoxt 166 6 cesium cesium NN cord-283998-whwksoxt 166 7 sulfate sulfate NN cord-283998-whwksoxt 166 8 gradient gradient NN cord-283998-whwksoxt 166 9 experiments experiment NNS cord-283998-whwksoxt 166 10 in in IN cord-283998-whwksoxt 166 11 which which WDT cord-283998-whwksoxt 166 12 the the DT cord-283998-whwksoxt 166 13 buoyant buoyant JJ cord-283998-whwksoxt 166 14 densities density NNS cord-283998-whwksoxt 166 15 of of IN cord-283998-whwksoxt 166 16 the the DT cord-283998-whwksoxt 166 17 OC-43 OC-43 NNP cord-283998-whwksoxt 166 18 complex complex NN cord-283998-whwksoxt 166 19 prepared prepare VBN cord-283998-whwksoxt 166 20 by by IN cord-283998-whwksoxt 166 21 SDS sds NN cord-283998-whwksoxt 166 22 lysis lysis NN cord-283998-whwksoxt 166 23 and and CC cord-283998-whwksoxt 166 24 phenol phenol NN cord-283998-whwksoxt 166 25 - - HYPH cord-283998-whwksoxt 166 26 extracted extract VBN cord-283998-whwksoxt 166 27 TMV TMV NNP cord-283998-whwksoxt 166 28 RNA RNA NNP cord-283998-whwksoxt 166 29 were be VBD cord-283998-whwksoxt 166 30 identical identical JJ cord-283998-whwksoxt 167 1 ( ( -LRB- cord-283998-whwksoxt 167 2 Tannock Tannock NNP cord-283998-whwksoxt 167 3 and and CC cord-283998-whwksoxt 167 4 Hierholzer Hierholzer NNP cord-283998-whwksoxt 167 5 , , , cord-283998-whwksoxt 167 6 unpublished unpublished JJ cord-283998-whwksoxt 167 7 observations observation NNS cord-283998-whwksoxt 167 8 ) ) -RRB- cord-283998-whwksoxt 167 9 . . . cord-283998-whwksoxt 168 1 Additionally additionally RB cord-283998-whwksoxt 168 2 , , , cord-283998-whwksoxt 168 3 Lowry Lowry NNP cord-283998-whwksoxt 168 4 protein protein NN cord-283998-whwksoxt 168 5 determinations determination NNS cord-283998-whwksoxt 168 6 and and CC cord-283998-whwksoxt 168 7 8 8 CD cord-283998-whwksoxt 168 8 % % NN cord-283998-whwksoxt 168 9 acrylamide acrylamide NN cord-283998-whwksoxt 168 10 gels gel NNS cord-283998-whwksoxt 168 11 ( ( -LRB- cord-283998-whwksoxt 168 12 protein protein NN cord-283998-whwksoxt 168 13 - - HYPH cord-283998-whwksoxt 168 14 stained stain VBN cord-283998-whwksoxt 168 15 ) ) -RRB- cord-283998-whwksoxt 168 16 on on IN cord-283998-whwksoxt 168 17 purified purify VBN cord-283998-whwksoxt 168 18 RNA RNA NNP cord-283998-whwksoxt 168 19 samples sample NNS cord-283998-whwksoxt 168 20 containing contain VBG cord-283998-whwksoxt 168 21 120 120 CD cord-283998-whwksoxt 168 22 pg pg NNP cord-283998-whwksoxt 168 23 RNA RNA NNP cord-283998-whwksoxt 168 24 did do VBD cord-283998-whwksoxt 168 25 not not RB cord-283998-whwksoxt 168 26 detect detect VB cord-283998-whwksoxt 168 27 protein protein NN cord-283998-whwksoxt 168 28 , , , cord-283998-whwksoxt 168 29 implying imply VBG cord-283998-whwksoxt 168 30 that that IN cord-283998-whwksoxt 168 31 any any DT cord-283998-whwksoxt 168 32 protein protein NN cord-283998-whwksoxt 168 33 linkers linker NNS cord-283998-whwksoxt 168 34 present present JJ cord-283998-whwksoxt 168 35 must must MD cord-283998-whwksoxt 168 36 constitute constitute VB cord-283998-whwksoxt 168 37 less less JJR cord-283998-whwksoxt 168 38 than than IN cord-283998-whwksoxt 168 39 2 2 CD cord-283998-whwksoxt 168 40 % % NN cord-283998-whwksoxt 168 41 of of IN cord-283998-whwksoxt 168 42 the the DT cord-283998-whwksoxt 168 43 RNA RNA NNP cord-283998-whwksoxt 168 44 structure structure NN cord-283998-whwksoxt 168 45 . . . cord-283998-whwksoxt 169 1 The the DT cord-283998-whwksoxt 169 2 complexing complexing NN cord-283998-whwksoxt 169 3 of of IN cord-283998-whwksoxt 169 4 a a DT cord-283998-whwksoxt 169 5 few few JJ cord-283998-whwksoxt 169 6 small small JJ cord-283998-whwksoxt 169 7 proteins protein NNS cord-283998-whwksoxt 169 8 to to IN cord-283998-whwksoxt 169 9 a a DT cord-283998-whwksoxt 169 10 large large JJ cord-283998-whwksoxt 169 11 RNA RNA NNP cord-283998-whwksoxt 169 12 molecule molecule NN cord-283998-whwksoxt 169 13 , , , cord-283998-whwksoxt 169 14 however however RB cord-283998-whwksoxt 169 15 , , , cord-283998-whwksoxt 169 16 may may MD cord-283998-whwksoxt 169 17 produce produce VB cord-283998-whwksoxt 169 18 no no DT cord-283998-whwksoxt 169 19 detectable detectable JJ cord-283998-whwksoxt 169 20 difference difference NN cord-283998-whwksoxt 169 21 in in IN cord-283998-whwksoxt 169 22 buoyant buoyant JJ cord-283998-whwksoxt 169 23 density density NN cord-283998-whwksoxt 169 24 , , , cord-283998-whwksoxt 169 25 as as IN cord-283998-whwksoxt 169 26 Sen Sen NNP cord-283998-whwksoxt 169 27 et et NNP cord-283998-whwksoxt 169 28 aZ. az. NN cord-283998-whwksoxt 169 29 ( ( -LRB- cord-283998-whwksoxt 169 30 1976 1976 CD cord-283998-whwksoxt 169 31 ) ) -RRB- cord-283998-whwksoxt 169 32 have have VBP cord-283998-whwksoxt 169 33 noted note VBN cord-283998-whwksoxt 169 34 for for IN cord-283998-whwksoxt 169 35 Rauscher Rauscher NNP cord-283998-whwksoxt 169 36 murine murine JJ cord-283998-whwksoxt 169 37 leukemia leukemia NN cord-283998-whwksoxt 169 38 viral viral JJ cord-283998-whwksoxt 169 39 RNA RNA NNP cord-283998-whwksoxt 169 40 and and CC cord-283998-whwksoxt 169 41 phosphoprotein phosphoprotein NN cord-283998-whwksoxt 169 42 P12 p12 NN cord-283998-whwksoxt 169 43 , , , cord-283998-whwksoxt 169 44 with with IN cord-283998-whwksoxt 169 45 a a DT cord-283998-whwksoxt 169 46 type type NN cord-283998-whwksoxt 169 47 - - HYPH cord-283998-whwksoxt 169 48 specific specific JJ cord-283998-whwksoxt 169 49 RNA RNA NNP cord-283998-whwksoxt 169 50 binding bind VBG cord-283998-whwksoxt 169 51 affinity affinity NN cord-283998-whwksoxt 169 52 . . . cord-283998-whwksoxt 170 1 Extensive extensive JJ cord-283998-whwksoxt 170 2 nicking nicking NN cord-283998-whwksoxt 170 3 of of IN cord-283998-whwksoxt 170 4 retroviral retroviral JJ cord-283998-whwksoxt 170 5 70 70 CD cord-283998-whwksoxt 170 6 S S NNP cord-283998-whwksoxt 170 7 RNA RNA NNP cord-283998-whwksoxt 170 8 due due IN cord-283998-whwksoxt 170 9 to to IN cord-283998-whwksoxt 170 10 ribonuclease ribonuclease NN cord-283998-whwksoxt 170 11 activity activity NN cord-283998-whwksoxt 170 12 has have VBZ cord-283998-whwksoxt 170 13 been be VBN cord-283998-whwksoxt 170 14 observed observe VBN cord-283998-whwksoxt 170 15 after after IN cord-283998-whwksoxt 170 16 extended extended JJ cord-283998-whwksoxt 170 17 viral viral JJ cord-283998-whwksoxt 170 18 growth growth NN cord-283998-whwksoxt 170 19 in in IN cord-283998-whwksoxt 170 20 cell cell NN cord-283998-whwksoxt 170 21 culture culture NN cord-283998-whwksoxt 170 22 ( ( -LRB- cord-283998-whwksoxt 170 23 Bader Bader NNP cord-283998-whwksoxt 170 24 and and CC cord-283998-whwksoxt 170 25 Steck Steck NNP cord-283998-whwksoxt 170 26 , , , cord-283998-whwksoxt 170 27 1969 1969 CD cord-283998-whwksoxt 170 28 ; ; : cord-283998-whwksoxt 170 29 King King NNP cord-283998-whwksoxt 170 30 and and CC cord-283998-whwksoxt 170 31 Wells Wells NNP cord-283998-whwksoxt 170 32 , , , cord-283998-whwksoxt 170 33 1976 1976 CD cord-283998-whwksoxt 170 34 ) ) -RRB- cord-283998-whwksoxt 170 35 and and CC cord-283998-whwksoxt 170 36 during during IN cord-283998-whwksoxt 170 37 virus virus NN cord-283998-whwksoxt 170 38 purifcation purifcation NN cord-283998-whwksoxt 170 39 ( ( -LRB- cord-283998-whwksoxt 170 40 Scheele Scheele NNP cord-283998-whwksoxt 170 41 and and CC cord-283998-whwksoxt 170 42 Hanafusa Hanafusa NNP cord-283998-whwksoxt 170 43 , , , cord-283998-whwksoxt 170 44 1972 1972 CD cord-283998-whwksoxt 170 45 ) ) -RRB- cord-283998-whwksoxt 170 46 . . . cord-283998-whwksoxt 171 1 The the DT cord-283998-whwksoxt 171 2 integrity integrity NN cord-283998-whwksoxt 171 3 of of IN cord-283998-whwksoxt 171 4 the the DT cord-283998-whwksoxt 171 5 nicked nicked JJ cord-283998-whwksoxt 171 6 70 70 CD cord-283998-whwksoxt 171 7 S S NNP cord-283998-whwksoxt 171 8 RNA RNA NNP cord-283998-whwksoxt 171 9 is be VBZ cord-283998-whwksoxt 171 10 still still RB cord-283998-whwksoxt 171 11 maintained maintain VBN cord-283998-whwksoxt 171 12 after after IN cord-283998-whwksoxt 171 13 extraction extraction NN cord-283998-whwksoxt 171 14 from from IN cord-283998-whwksoxt 171 15 virions virion NNS cord-283998-whwksoxt 171 16 by by IN cord-283998-whwksoxt 171 17 phenol phenol NN cord-283998-whwksoxt 171 18 , , , cord-283998-whwksoxt 171 19 being be VBG cord-283998-whwksoxt 171 20 only only RB cord-283998-whwksoxt 171 21 apparent apparent JJ cord-283998-whwksoxt 171 22 after after IN cord-283998-whwksoxt 171 23 treatment treatment NN cord-283998-whwksoxt 171 24 with with IN cord-283998-whwksoxt 171 25 RNA RNA NNP cord-283998-whwksoxt 171 26 denaturants denaturant NNS cord-283998-whwksoxt 171 27 . . . cord-283998-whwksoxt 172 1 Extensive extensive JJ cord-283998-whwksoxt 172 2 changes change NNS cord-283998-whwksoxt 172 3 in in IN cord-283998-whwksoxt 172 4 OC-43 OC-43 NNP cord-283998-whwksoxt 172 5 RNA RNA NNP cord-283998-whwksoxt 172 6 conformation conformation NN cord-283998-whwksoxt 172 7 produced produce VBN cord-283998-whwksoxt 172 8 by by IN cord-283998-whwksoxt 172 9 phenol phenol NN cord-283998-whwksoxt 172 10 suggest suggest VBP cord-283998-whwksoxt 172 11 that that IN cord-283998-whwksoxt 172 12 , , , cord-283998-whwksoxt 172 13 even even RB cord-283998-whwksoxt 172 14 if if IN cord-283998-whwksoxt 172 15 extensive extensive JJ cord-283998-whwksoxt 172 16 nicking nicking NN cord-283998-whwksoxt 172 17 of of IN cord-283998-whwksoxt 172 18 the the DT cord-283998-whwksoxt 172 19 70 70 CD cord-283998-whwksoxt 172 20 S S NNP cord-283998-whwksoxt 172 21 complex complex NN cord-283998-whwksoxt 172 22 occurs occur VBZ cord-283998-whwksoxt 172 23 during during IN cord-283998-whwksoxt 172 24 viral viral JJ cord-283998-whwksoxt 172 25 growth growth NN cord-283998-whwksoxt 172 26 , , , cord-283998-whwksoxt 172 27 there there EX cord-283998-whwksoxt 172 28 are be VBP cord-283998-whwksoxt 172 29 marked mark VBN cord-283998-whwksoxt 172 30 differences difference NNS cord-283998-whwksoxt 172 31 in in IN cord-283998-whwksoxt 172 32 its -PRON- PRP$ cord-283998-whwksoxt 172 33 secondary secondary JJ cord-283998-whwksoxt 172 34 structure structure NN cord-283998-whwksoxt 172 35 compared compare VBN cord-283998-whwksoxt 172 36 to to IN cord-283998-whwksoxt 172 37 that that DT cord-283998-whwksoxt 172 38 of of IN cord-283998-whwksoxt 172 39 70 70 CD cord-283998-whwksoxt 172 40 S S NNP cord-283998-whwksoxt 172 41 retroviral retroviral NN cord-283998-whwksoxt 172 42 RNA RNA NNP cord-283998-whwksoxt 172 43 . . . cord-283998-whwksoxt 173 1 To to TO cord-283998-whwksoxt 173 2 determine determine VB cord-283998-whwksoxt 173 3 whether whether IN cord-283998-whwksoxt 173 4 a a DT cord-283998-whwksoxt 173 5 genuine genuine JJ cord-283998-whwksoxt 173 6 subunit subunit NN cord-283998-whwksoxt 173 7 structure structure NN cord-283998-whwksoxt 173 8 is be VBZ cord-283998-whwksoxt 173 9 present present JJ cord-283998-whwksoxt 173 10 in in IN cord-283998-whwksoxt 173 11 the the DT cord-283998-whwksoxt 173 12 coronaviral coronaviral JJ cord-283998-whwksoxt 173 13 complex complex NN cord-283998-whwksoxt 173 14 , , , cord-283998-whwksoxt 173 15 it -PRON- PRP cord-283998-whwksoxt 173 16 would would MD cord-283998-whwksoxt 173 17 be be VB cord-283998-whwksoxt 173 18 highly highly RB cord-283998-whwksoxt 173 19 desirable desirable JJ cord-283998-whwksoxt 173 20 to to TO cord-283998-whwksoxt 173 21 examine examine VB cord-283998-whwksoxt 173 22 the the DT cord-283998-whwksoxt 173 23 virion virion NN cord-283998-whwksoxt 173 24 RNA RNA NNP cord-283998-whwksoxt 173 25 present present JJ cord-283998-whwksoxt 173 26 at at IN cord-283998-whwksoxt 173 27 different different JJ cord-283998-whwksoxt 173 28 times time NNS cord-283998-whwksoxt 173 29 after after IN cord-283998-whwksoxt 173 30 maturation maturation NN cord-283998-whwksoxt 173 31 in in IN cord-283998-whwksoxt 173 32 a a DT cord-283998-whwksoxt 173 33 more more RBR cord-283998-whwksoxt 173 34 strictly strictly RB cord-283998-whwksoxt 173 35 defined define VBN cord-283998-whwksoxt 173 36 milieu milieu NN cord-283998-whwksoxt 173 37 than than IN cord-283998-whwksoxt 173 38 that that DT cord-283998-whwksoxt 173 39 of of IN cord-283998-whwksoxt 173 40 the the DT cord-283998-whwksoxt 173 41 mouse mouse NN cord-283998-whwksoxt 173 42 brain brain NN cord-283998-whwksoxt 173 43 . . . cord-283998-whwksoxt 174 1 However however RB cord-283998-whwksoxt 174 2 , , , cord-283998-whwksoxt 174 3 for for IN cord-283998-whwksoxt 174 4 human human JJ cord-283998-whwksoxt 174 5 coronaviruses coronaviruse NNS cord-283998-whwksoxt 174 6 , , , cord-283998-whwksoxt 174 7 the the DT cord-283998-whwksoxt 174 8 lack lack NN cord-283998-whwksoxt 174 9 of of IN cord-283998-whwksoxt 174 10 convenient convenient JJ cord-283998-whwksoxt 174 11 model model NN cord-283998-whwksoxt 174 12 systems system NNS cord-283998-whwksoxt 174 13 is be VBZ cord-283998-whwksoxt 174 14 a a DT cord-283998-whwksoxt 174 15 severe severe JJ cord-283998-whwksoxt 174 16 impediment impediment NN cord-283998-whwksoxt 174 17 to to IN cord-283998-whwksoxt 174 18 this this DT cord-283998-whwksoxt 174 19 work work NN cord-283998-whwksoxt 174 20 . . . cord-283998-whwksoxt 175 1 Coronaviruses Coronaviruses NNP cord-283998-whwksoxt 175 2 Analysis Analysis NNP cord-283998-whwksoxt 175 3 of of IN cord-283998-whwksoxt 175 4 the the DT cord-283998-whwksoxt 175 5 ribonucleic ribonucleic JJ cord-283998-whwksoxt 175 6 acid acid NN cord-283998-whwksoxt 175 7 of of IN cord-283998-whwksoxt 175 8 murine murine JJ cord-283998-whwksoxt 175 9 leukemia leukemia NN cord-283998-whwksoxt 175 10 virus virus NN cord-283998-whwksoxt 175 11 Tumor tumor NN cord-283998-whwksoxt 175 12 viruses virus NNS cord-283998-whwksoxt 175 13 : : : cord-283998-whwksoxt 175 14 1974 1974 CD cord-283998-whwksoxt 175 15 . . . cord-283998-whwksoxt 176 1 Cold cold JJ cord-283998-whwksoxt 176 2 Spring Spring NNP cord-283998-whwksoxt 176 3 Harbor Harbor NNP cord-283998-whwksoxt 176 4 Symp Symp NNP cord-283998-whwksoxt 177 1 The the DT cord-283998-whwksoxt 177 2 low low JJ cord-283998-whwksoxt 177 3 molecular molecular JJ cord-283998-whwksoxt 177 4 weight weight NN cord-283998-whwksoxt 177 5 RNAs rna NNS cord-283998-whwksoxt 177 6 of of IN cord-283998-whwksoxt 177 7 Rous rous JJ cord-283998-whwksoxt 177 8 sarcoma sarcoma NN cord-283998-whwksoxt 177 9 virus virus NN cord-283998-whwksoxt 177 10 . . . cord-283998-whwksoxt 178 1 I. I. NNP cord-283998-whwksoxt 179 1 The the DT cord-283998-whwksoxt 179 2 4s 4s CD cord-283998-whwksoxt 179 3 RNA RNA NNP cord-283998-whwksoxt 179 4 Forces Forces NNPS cord-283998-whwksoxt 179 5 responsible responsible JJ cord-283998-whwksoxt 179 6 for for IN cord-283998-whwksoxt 179 7 the the DT cord-283998-whwksoxt 179 8 generation generation NN cord-283998-whwksoxt 179 9 of of IN cord-283998-whwksoxt 179 10 virus virus NN cord-283998-whwksoxt 179 11 structures structure NNS cord-283998-whwksoxt 179 12 : : : cord-283998-whwksoxt 179 13 The the DT cord-283998-whwksoxt 179 14 use use NN cord-283998-whwksoxt 179 15 of of IN cord-283998-whwksoxt 179 16 SDS SDS NNP cord-283998-whwksoxt 179 17 to to TO cord-283998-whwksoxt 179 18 probe probe VB cord-283998-whwksoxt 179 19 protein protein NN cord-283998-whwksoxt 179 20 - - HYPH cord-283998-whwksoxt 179 21 RNA RNA NNP cord-283998-whwksoxt 179 22 interaction interaction NN cord-283998-whwksoxt 179 23 Structure structure NN cord-283998-whwksoxt 179 24 and and CC cord-283998-whwksoxt 179 25 properties property NNS cord-283998-whwksoxt 179 26 of of IN cord-283998-whwksoxt 179 27 an an DT cord-283998-whwksoxt 179 28 RNA RNA NNP cord-283998-whwksoxt 179 29 primer primer NN cord-283998-whwksoxt 179 30 for for IN cord-283998-whwksoxt 179 31 initiation initiation NN cord-283998-whwksoxt 179 32 of of IN cord-283998-whwksoxt 179 33 Rous rous JJ cord-283998-whwksoxt 179 34 sarcoma sarcoma NN cord-283998-whwksoxt 179 35 virus virus NN cord-283998-whwksoxt 179 36 DNA dna NN cord-283998-whwksoxt 179 37 synthesis synthesis NN cord-283998-whwksoxt 179 38 in in IN cord-283998-whwksoxt 179 39 vitro vitro FW cord-283998-whwksoxt 179 40 Physical physical JJ cord-283998-whwksoxt 179 41 properties property NNS cord-283998-whwksoxt 179 42 of of IN cord-283998-whwksoxt 179 43 Rous rous JJ cord-283998-whwksoxt 179 44 sarcoma sarcoma NN cord-283998-whwksoxt 179 45 virus virus NN cord-283998-whwksoxt 179 46 RNA RNA NNP cord-283998-whwksoxt 179 47 Identification Identification NNP cord-283998-whwksoxt 179 48 of of IN cord-283998-whwksoxt 179 49 heat heat NN cord-283998-whwksoxt 179 50 - - HYPH cord-283998-whwksoxt 179 51 dissociable dissociable NNP cord-283998-whwksoxt 179 52 RNA RNA NNP cord-283998-whwksoxt 179 53 complexes complex NNS cord-283998-whwksoxt 179 54 in in IN cord-283998-whwksoxt 179 55 two two CD cord-283998-whwksoxt 179 56 porcine porcine JJ cord-283998-whwksoxt 179 57 coronaviruses coronaviruse NNS cord-283998-whwksoxt 179 58 Purification purification NN cord-283998-whwksoxt 179 59 and and CC cord-283998-whwksoxt 179 60 biophysical biophysical JJ cord-283998-whwksoxt 179 61 properties property NNS cord-283998-whwksoxt 179 62 of of IN cord-283998-whwksoxt 179 63 human human JJ cord-283998-whwksoxt 179 64 coronavirus coronavirus NN cord-283998-whwksoxt 179 65 2293 2293 CD cord-283998-whwksoxt 179 66 Protein Protein NNP cord-283998-whwksoxt 179 67 composition composition NN cord-283998-whwksoxt 179 68 of of IN cord-283998-whwksoxt 179 69 coronavirus coronavirus NN cord-283998-whwksoxt 179 70 OC-43 OC-43 NNP cord-283998-whwksoxt 179 71 Standardized Standardized NNP cord-283998-whwksoxt 179 72 viral viral JJ cord-283998-whwksoxt 179 73 hemagglutination hemagglutination NN cord-283998-whwksoxt 179 74 and and CC cord-283998-whwksoxt 179 75 hemagglutination hemagglutination NN cord-283998-whwksoxt 179 76 - - HYPH cord-283998-whwksoxt 179 77 inhibition inhibition NN cord-283998-whwksoxt 179 78 tests test NNS cord-283998-whwksoxt 179 79 . . . cord-283998-whwksoxt 180 1 II II NNP cord-283998-whwksoxt 180 2 . . . cord-283998-whwksoxt 181 1 Description description NN cord-283998-whwksoxt 181 2 and and CC cord-283998-whwksoxt 181 3 statistical statistical JJ cord-283998-whwksoxt 181 4 evaluation evaluation NN cord-283998-whwksoxt 181 5 Purification purification NN cord-283998-whwksoxt 181 6 and and CC cord-283998-whwksoxt 181 7 further further JJ cord-283998-whwksoxt 181 8 characterization characterization NN cord-283998-whwksoxt 181 9 of of IN cord-283998-whwksoxt 181 10 an an DT cord-283998-whwksoxt 181 11 " " `` cord-283998-whwksoxt 181 12 IBV IBV NNP cord-283998-whwksoxt 181 13 - - HYPH cord-283998-whwksoxt 181 14 like like JJ cord-283998-whwksoxt 181 15 " " '' cord-283998-whwksoxt 181 16 virus virus NN cord-283998-whwksoxt 181 17 ( ( -LRB- cord-283998-whwksoxt 181 18 coronavirus coronavirus NN cord-283998-whwksoxt 181 19 ) ) -RRB- cord-283998-whwksoxt 181 20 High high JJ cord-283998-whwksoxt 181 21 molecular molecular JJ cord-283998-whwksoxt 181 22 weight weight NN cord-283998-whwksoxt 181 23 RNAs rna NNS cord-283998-whwksoxt 181 24 from from IN cord-283998-whwksoxt 181 25 Rous rous JJ cord-283998-whwksoxt 181 26 sarcoma sarcoma NN cord-283998-whwksoxt 181 27 virus virus NN cord-283998-whwksoxt 181 28 and and CC cord-283998-whwksoxt 181 29 Moloney Moloney NNP cord-283998-whwksoxt 181 30 murine murine JJ cord-283998-whwksoxt 181 31 leukemia leukemia NN cord-283998-whwksoxt 181 32 virus virus NN cord-283998-whwksoxt 181 33 contain contain VBP cord-283998-whwksoxt 181 34 two two CD cord-283998-whwksoxt 181 35 subunits subunit NNS cord-283998-whwksoxt 182 1 All all DT cord-283998-whwksoxt 182 2 intact intact JJ cord-283998-whwksoxt 182 3 subunit subunit NN cord-283998-whwksoxt 182 4 RNAs rna NNS cord-283998-whwksoxt 182 5 from from IN cord-283998-whwksoxt 182 6 Rous rous JJ cord-283998-whwksoxt 182 7 sarcoma sarcoma NN cord-283998-whwksoxt 182 8 virus virus NN cord-283998-whwksoxt 182 9 contain contain VBP cord-283998-whwksoxt 182 10 poly(A poly(a JJ cord-283998-whwksoxt 182 11 ) ) -RRB- cord-283998-whwksoxt 183 1 Molecular molecular JJ cord-283998-whwksoxt 183 2 weights weight NNS cord-283998-whwksoxt 183 3 of of IN cord-283998-whwksoxt 183 4 ribosomal ribosomal JJ cord-283998-whwksoxt 183 5 RNA RNA NNP cord-283998-whwksoxt 183 6 in in IN cord-283998-whwksoxt 183 7 relation relation NN cord-283998-whwksoxt 183 8 to to IN cord-283998-whwksoxt 183 9 evolution evolution NN cord-283998-whwksoxt 184 1 The the DT cord-283998-whwksoxt 184 2 determination determination NN cord-283998-whwksoxt 184 3 of of IN cord-283998-whwksoxt 184 4 the the DT cord-283998-whwksoxt 184 5 molecular molecular JJ cord-283998-whwksoxt 184 6 weight weight NN cord-283998-whwksoxt 184 7 of of IN cord-283998-whwksoxt 184 8 ribonucleic ribonucleic JJ cord-283998-whwksoxt 184 9 acid acid NN cord-283998-whwksoxt 184 10 by by IN cord-283998-whwksoxt 184 11 polyacrylamide polyacrylamide NN cord-283998-whwksoxt 184 12 - - HYPH cord-283998-whwksoxt 184 13 gel gel NN cord-283998-whwksoxt 184 14 electrophoresis electrophoresis NN cord-283998-whwksoxt 184 15 . . . cord-283998-whwksoxt 185 1 The the DT cord-283998-whwksoxt 185 2 effects effect NNS cord-283998-whwksoxt 185 3 of of IN cord-283998-whwksoxt 185 4 changes change NNS cord-283998-whwksoxt 185 5 in in IN cord-283998-whwksoxt 185 6 conformation conformation NN cord-283998-whwksoxt 185 7 Molecular Molecular NNP cord-283998-whwksoxt 185 8 weights weight NNS cord-283998-whwksoxt 185 9 of of IN cord-283998-whwksoxt 185 10 some some DT cord-283998-whwksoxt 185 11 HeLa HeLa NNP cord-283998-whwksoxt 185 12 ribosomal ribosomal JJ cord-283998-whwksoxt 185 13 RNAs RNAs NNP cord-283998-whwksoxt 185 14 Growth Growth NNP cord-283998-whwksoxt 185 15 in in IN cord-283998-whwksoxt 185 16 suckling suckle VBG cord-283998-whwksoxt 185 17 - - HYPH cord-283998-whwksoxt 185 18 mouse mouse NN cord-283998-whwksoxt 185 19 brain brain NN cord-283998-whwksoxt 185 20 of of IN cord-283998-whwksoxt 185 21 " " `` cord-283998-whwksoxt 185 22 IBVlike IBVlike NNP cord-283998-whwksoxt 185 23 " " '' cord-283998-whwksoxt 185 24 viruses virus NNS cord-283998-whwksoxt 185 25 from from IN cord-283998-whwksoxt 185 26 patients patient NNS cord-283998-whwksoxt 185 27 with with IN cord-283998-whwksoxt 185 28 upper upper JJ cord-283998-whwksoxt 185 29 respiratory respiratory JJ cord-283998-whwksoxt 185 30 tract tract NN cord-283998-whwksoxt 185 31 disease disease NN cord-283998-whwksoxt 185 32 Arrangement arrangement NN cord-283998-whwksoxt 185 33 of of IN cord-283998-whwksoxt 185 34 specific specific JJ cord-283998-whwksoxt 185 35 oligonucleotides oligonucleotide NNS cord-283998-whwksoxt 185 36 within within IN cord-283998-whwksoxt 185 37 poly(A poly(A NNP cord-283998-whwksoxt 185 38 ) ) -RRB- cord-283998-whwksoxt 185 39 terminated terminate VBD cord-283998-whwksoxt 185 40 Hn Hn NNP cord-283998-whwksoxt 185 41 RNA RNA NNP cord-283998-whwksoxt 185 42 molecules molecule NNS cord-283998-whwksoxt 186 1 The the DT cord-283998-whwksoxt 186 2 influence influence NN cord-283998-whwksoxt 186 3 of of IN cord-283998-whwksoxt 186 4 pH ph NN cord-283998-whwksoxt 186 5 on on IN cord-283998-whwksoxt 186 6 the the DT cord-283998-whwksoxt 186 7 growth growth NN cord-283998-whwksoxt 186 8 and and CC cord-283998-whwksoxt 186 9 stability stability NN cord-283998-whwksoxt 186 10 of of IN cord-283998-whwksoxt 186 11 transmissable transmissable JJ cord-283998-whwksoxt 186 12 gastroenteritis gastroenteritis NN cord-283998-whwksoxt 186 13 virus virus NN cord-283998-whwksoxt 186 14 in in IN cord-283998-whwksoxt 186 15 vitro vitro FW cord-283998-whwksoxt 186 16 Intbrenza Intbrenza NNP cord-283998-whwksoxt 186 17 virus virus NN cord-283998-whwksoxt 186 18 messenger messenger NN cord-283998-whwksoxt 186 19 ribonucleoprotein ribonucleoprotein NNP cord-283998-whwksoxt 186 20 Determination Determination NNP cord-283998-whwksoxt 186 21 of of IN cord-283998-whwksoxt 186 22 the the DT cord-283998-whwksoxt 186 23 molecular molecular JJ cord-283998-whwksoxt 186 24 weight weight NN cord-283998-whwksoxt 186 25 of of IN cord-283998-whwksoxt 186 26 animal animal NN cord-283998-whwksoxt 186 27 RNA rna NN cord-283998-whwksoxt 186 28 viral viral JJ cord-283998-whwksoxt 186 29 genomes genome NNS cord-283998-whwksoxt 186 30 by by IN cord-283998-whwksoxt 186 31 nuclease nuclease NN cord-283998-whwksoxt 186 32 digestions digestion NNS cord-283998-whwksoxt 186 33 . . . cord-283998-whwksoxt 187 1 I. I. NNP cord-283998-whwksoxt 187 2 Vesicular Vesicular NNP cord-283998-whwksoxt 187 3 stomatitis stomatitis NN cord-283998-whwksoxt 187 4 virus virus NN cord-283998-whwksoxt 187 5 and and CC cord-283998-whwksoxt 187 6 its -PRON- PRP$ cord-283998-whwksoxt 187 7 defective defective JJ cord-283998-whwksoxt 187 8 T t NN cord-283998-whwksoxt 187 9 particle particle NN cord-283998-whwksoxt 188 1 The the DT cord-283998-whwksoxt 188 2 nucleic nucleic JJ cord-283998-whwksoxt 188 3 acid acid NN cord-283998-whwksoxt 188 4 of of IN cord-283998-whwksoxt 188 5 the the DT cord-283998-whwksoxt 188 6 Bryan Bryan NNP cord-283998-whwksoxt 188 7 strain strain NN cord-283998-whwksoxt 188 8 cf cf NNP cord-283998-whwksoxt 188 9 Rous rous JJ cord-283998-whwksoxt 188 10 sarcoma sarcoma NN cord-283998-whwksoxt 188 11 virus virus NN cord-283998-whwksoxt 188 12 : : : cord-283998-whwksoxt 189 1 Purification purification NN cord-283998-whwksoxt 189 2 of of IN cord-283998-whwksoxt 189 3 the the DT cord-283998-whwksoxt 189 4 virus virus NN cord-283998-whwksoxt 189 5 and and CC cord-283998-whwksoxt 189 6 isolation isolation NN cord-283998-whwksoxt 189 7 of of IN cord-283998-whwksoxt 189 8 the the DT cord-283998-whwksoxt 189 9 nucleic nucleic JJ cord-283998-whwksoxt 189 10 acid acid NN cord-283998-whwksoxt 189 11 Electrophoretie Electrophoretie NNP cord-283998-whwksoxt 189 12 analysis analysis NN cord-283998-whwksoxt 189 13 of of IN cord-283998-whwksoxt 189 14 the the DT cord-283998-whwksoxt 189 15 RNA RNA NNP cord-283998-whwksoxt 189 16 of of IN cord-283998-whwksoxt 189 17 avian avian JJ cord-283998-whwksoxt 189 18 tumor tumor NN cord-283998-whwksoxt 189 19 viruses virus NNS cord-283998-whwksoxt 190 1 Specific specific JJ cord-283998-whwksoxt 190 2 binding binding NN cord-283998-whwksoxt 190 3 of of IN cord-283998-whwksoxt 190 4 the the DT cord-283998-whwksoxt 190 5 type type NN cord-283998-whwksoxt 190 6 C C NNP cord-283998-whwksoxt 190 7 viral viral JJ cord-283998-whwksoxt 190 8 core core NN cord-283998-whwksoxt 190 9 protein protein NN cord-283998-whwksoxt 190 10 p12 p12 NN cord-283998-whwksoxt 190 11 with with IN cord-283998-whwksoxt 190 12 purified purify VBN cord-283998-whwksoxt 190 13 viral viral JJ cord-283998-whwksoxt 190 14 RNA RNA NNP cord-283998-whwksoxt 190 15 Molecular Molecular NNP cord-283998-whwksoxt 190 16 weight weight NN cord-283998-whwksoxt 190 17 determination determination NN cord-283998-whwksoxt 190 18 of of IN cord-283998-whwksoxt 190 19 nucleic nucleic JJ cord-283998-whwksoxt 190 20 acids acid NNS cord-283998-whwksoxt 190 21 by by IN cord-283998-whwksoxt 190 22 gel gel NN cord-283998-whwksoxt 190 23 electrophoresis electrophoresis NN cord-283998-whwksoxt 190 24 in in IN cord-283998-whwksoxt 190 25 non non JJ cord-283998-whwksoxt 190 26 - - JJ cord-283998-whwksoxt 190 27 aqueous aqueous JJ cord-283998-whwksoxt 190 28 solution solution NN cord-283998-whwksoxt 190 29 Structure Structure NNP cord-283998-whwksoxt 190 30 of of IN cord-283998-whwksoxt 190 31 B77 B77 NNP cord-283998-whwksoxt 190 32 sarcoma sarcoma NN cord-283998-whwksoxt 190 33 virus virus NN cord-283998-whwksoxt 190 34 RNA rna NN cord-283998-whwksoxt 190 35 : : : cord-283998-whwksoxt 190 36 Stabilization Stabilization NNP cord-283998-whwksoxt 190 37 of of IN cord-283998-whwksoxt 190 38 RNA RNA NNP cord-283998-whwksoxt 190 39 after after IN cord-283998-whwksoxt 190 40 packaging package VBG cord-283998-whwksoxt 190 41 The the DT cord-283998-whwksoxt 190 42 nucleic nucleic JJ cord-283998-whwksoxt 190 43 acid acid NN cord-283998-whwksoxt 190 44 ofinfectious ofinfectious JJ cord-283998-whwksoxt 190 45 bronchitis bronchitis NN cord-283998-whwksoxt 190 46 virus virus NN cord-283998-whwksoxt 191 1 A a DT cord-283998-whwksoxt 191 2 re re NN cord-283998-whwksoxt 191 3 - - NN cord-283998-whwksoxt 191 4 examination examination NN cord-283998-whwksoxt 191 5 of of IN cord-283998-whwksoxt 191 6 the the DT cord-283998-whwksoxt 191 7 molecular molecular JJ cord-283998-whwksoxt 191 8 weight weight NN cord-283998-whwksoxt 191 9 of of IN cord-283998-whwksoxt 191 10 poliovirus poliovirus NN cord-283998-whwksoxt 191 11 RNA RNA NNP cord-283998-whwksoxt 191 12 Degradation Degradation NNP cord-283998-whwksoxt 191 13 of of IN cord-283998-whwksoxt 191 14 myxovirus myxovirus NNP cord-283998-whwksoxt 191 15 virion virion NN cord-283998-whwksoxt 191 16 RNA RNA NNP cord-283998-whwksoxt 191 17 by by IN cord-283998-whwksoxt 191 18 periodate periodate NNP cord-283998-whwksoxt 191 19 The the DT cord-283998-whwksoxt 191 20 ribonucleic ribonucleic JJ cord-283998-whwksoxt 191 21 acid acid NN cord-283998-whwksoxt 191 22 of of IN cord-283998-whwksoxt 191 23 infectious infectious JJ cord-283998-whwksoxt 191 24 bronchitis bronchitis NN cord-283998-whwksoxt 191 25 virus virus NN