id	sid	tid	token	lemma	pos
cord-288669-46tkedw7	1	1	key	key	JJ
cord-288669-46tkedw7	1	2	:	:	:
cord-288669-46tkedw7	1	3	cord-288669	cord-288669	NNP
cord-288669-46tkedw7	1	4	-	-	HYPH
cord-288669-46tkedw7	1	5	46tkedw7	46tkedw7	CD
cord-288669-46tkedw7	1	6	authors	author	NNS
cord-288669-46tkedw7	1	7	:	:	:
cord-288669-46tkedw7	1	8	Lee	Lee	NNP
cord-288669-46tkedw7	1	9	,	,	,
cord-288669-46tkedw7	1	10	Changhee	Changhee	NNP
cord-288669-46tkedw7	1	11	;	;	:
cord-288669-46tkedw7	1	12	Yoo	Yoo	NNP
cord-288669-46tkedw7	1	13	,	,	,
cord-288669-46tkedw7	2	1	Dongwan	Dongwan	NNP
cord-288669-46tkedw7	2	2	title	title	NN
cord-288669-46tkedw7	2	3	:	:	:
cord-288669-46tkedw7	3	1	The	the	DT
cord-288669-46tkedw7	3	2	small	small	JJ
cord-288669-46tkedw7	3	3	envelope	envelope	NN
cord-288669-46tkedw7	3	4	protein	protein	NN
cord-288669-46tkedw7	3	5	of	of	IN
cord-288669-46tkedw7	3	6	porcine	porcine	JJ
cord-288669-46tkedw7	3	7	reproductive	reproductive	JJ
cord-288669-46tkedw7	3	8	and	and	CC
cord-288669-46tkedw7	3	9	respiratory	respiratory	JJ
cord-288669-46tkedw7	3	10	syndrome	syndrome	NN
cord-288669-46tkedw7	3	11	virus	virus	NN
cord-288669-46tkedw7	3	12	possesses	possess	VBZ
cord-288669-46tkedw7	3	13	ion	ion	NN
cord-288669-46tkedw7	3	14	channel	channel	NN
cord-288669-46tkedw7	3	15	protein	protein	NN
cord-288669-46tkedw7	3	16	-	-	HYPH
cord-288669-46tkedw7	3	17	like	like	JJ
cord-288669-46tkedw7	3	18	properties	property	NNS
cord-288669-46tkedw7	3	19	date	date	NN
cord-288669-46tkedw7	3	20	:	:	:
cord-288669-46tkedw7	3	21	2006	2006	CD
cord-288669-46tkedw7	3	22	-	-	SYM
cord-288669-46tkedw7	3	23	11	11	CD
cord-288669-46tkedw7	3	24	-	-	SYM
cord-288669-46tkedw7	3	25	10	10	CD
cord-288669-46tkedw7	3	26	journal	journal	NN
cord-288669-46tkedw7	3	27	:	:	:
cord-288669-46tkedw7	3	28	Virology	Virology	NNP
cord-288669-46tkedw7	3	29	DOI	DOI	NNP
cord-288669-46tkedw7	3	30	:	:	:
cord-288669-46tkedw7	3	31	10.1016	10.1016	CD
cord-288669-46tkedw7	3	32	/	/	SYM
cord-288669-46tkedw7	3	33	j.virol.2006.07.013	j.virol.2006.07.013	NN
cord-288669-46tkedw7	3	34	sha	sha	NNP
cord-288669-46tkedw7	3	35	:	:	:
cord-288669-46tkedw7	4	1	8a51ddb8a816799d5f38c008a0c869bf83c9e50b	8a51ddb8a816799d5f38c008a0c869bf83c9e50b	NNP
cord-288669-46tkedw7	4	2	doc_id	doc_id	CD
cord-288669-46tkedw7	4	3	:	:	:
cord-288669-46tkedw7	4	4	288669	288669	CD
cord-288669-46tkedw7	4	5	cord_uid	cord_uid	NNS
cord-288669-46tkedw7	4	6	:	:	:
cord-288669-46tkedw7	4	7	46tkedw7	46tkedw7	CD
cord-288669-46tkedw7	5	1	The	the	DT
cord-288669-46tkedw7	5	2	small	small	JJ
cord-288669-46tkedw7	5	3	envelope	envelope	NN
cord-288669-46tkedw7	5	4	(	(	-LRB-
cord-288669-46tkedw7	5	5	E	e	NN
cord-288669-46tkedw7	5	6	)	)	-RRB-
cord-288669-46tkedw7	5	7	protein	protein	NN
cord-288669-46tkedw7	5	8	of	of	IN
cord-288669-46tkedw7	5	9	porcine	porcine	JJ
cord-288669-46tkedw7	5	10	reproductive	reproductive	JJ
cord-288669-46tkedw7	5	11	and	and	CC
cord-288669-46tkedw7	5	12	respiratory	respiratory	JJ
cord-288669-46tkedw7	5	13	syndrome	syndrome	NN
cord-288669-46tkedw7	5	14	virus	virus	NN
cord-288669-46tkedw7	5	15	(	(	-LRB-
cord-288669-46tkedw7	5	16	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	5	17	)	)	-RRB-
cord-288669-46tkedw7	5	18	is	be	VBZ
cord-288669-46tkedw7	5	19	a	a	DT
cord-288669-46tkedw7	5	20	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	5	21	73	73	CD
cord-288669-46tkedw7	5	22	amino	amino	NN
cord-288669-46tkedw7	5	23	acid	acid	NN
cord-288669-46tkedw7	5	24	protein	protein	NN
cord-288669-46tkedw7	5	25	encoded	encode	VBN
cord-288669-46tkedw7	5	26	in	in	IN
cord-288669-46tkedw7	5	27	the	the	DT
cord-288669-46tkedw7	5	28	internal	internal	JJ
cord-288669-46tkedw7	5	29	open	open	JJ
cord-288669-46tkedw7	5	30	reading	reading	NN
cord-288669-46tkedw7	5	31	frame	frame	NN
cord-288669-46tkedw7	5	32	(	(	-LRB-
cord-288669-46tkedw7	5	33	ORF	ORF	NNP
cord-288669-46tkedw7	5	34	)	)	-RRB-
cord-288669-46tkedw7	5	35	of	of	IN
cord-288669-46tkedw7	5	36	the	the	DT
cord-288669-46tkedw7	5	37	bicistronic	bicistronic	JJ
cord-288669-46tkedw7	5	38	mRNA2	mrna2	NN
cord-288669-46tkedw7	5	39	.	.	.
cord-288669-46tkedw7	6	1	As	as	IN
cord-288669-46tkedw7	6	2	a	a	DT
cord-288669-46tkedw7	6	3	first	first	JJ
cord-288669-46tkedw7	6	4	step	step	NN
cord-288669-46tkedw7	6	5	towards	towards	IN
cord-288669-46tkedw7	6	6	understanding	understand	VBG
cord-288669-46tkedw7	6	7	the	the	DT
cord-288669-46tkedw7	6	8	biological	biological	JJ
cord-288669-46tkedw7	6	9	role	role	NN
cord-288669-46tkedw7	6	10	of	of	IN
cord-288669-46tkedw7	6	11	E	e	NN
cord-288669-46tkedw7	6	12	protein	protein	NN
cord-288669-46tkedw7	6	13	during	during	IN
cord-288669-46tkedw7	6	14	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	6	15	replication	replication	NN
cord-288669-46tkedw7	6	16	,	,	,
cord-288669-46tkedw7	6	17	E	e	NN
cord-288669-46tkedw7	6	18	gene	gene	NN
cord-288669-46tkedw7	6	19	expression	expression	NN
cord-288669-46tkedw7	6	20	was	be	VBD
cord-288669-46tkedw7	6	21	blocked	block	VBN
cord-288669-46tkedw7	6	22	in	in	IN
cord-288669-46tkedw7	6	23	a	a	DT
cord-288669-46tkedw7	6	24	full	full	JJ
cord-288669-46tkedw7	6	25	-	-	HYPH
cord-288669-46tkedw7	6	26	length	length	NN
cord-288669-46tkedw7	6	27	infectious	infectious	JJ
cord-288669-46tkedw7	6	28	clone	clone	NN
cord-288669-46tkedw7	6	29	by	by	IN
cord-288669-46tkedw7	6	30	mutating	mutate	VBG
cord-288669-46tkedw7	6	31	the	the	DT
cord-288669-46tkedw7	6	32	ATG	ATG	NNP
cord-288669-46tkedw7	6	33	translational	translational	JJ
cord-288669-46tkedw7	6	34	initiation	initiation	NN
cord-288669-46tkedw7	6	35	to	to	IN
cord-288669-46tkedw7	6	36	GTG	GTG	NNP
cord-288669-46tkedw7	6	37	,	,	,
cord-288669-46tkedw7	6	38	such	such	JJ
cord-288669-46tkedw7	6	39	that	that	IN
cord-288669-46tkedw7	6	40	the	the	DT
cord-288669-46tkedw7	6	41	full	full	JJ
cord-288669-46tkedw7	6	42	-	-	HYPH
cord-288669-46tkedw7	6	43	length	length	NN
cord-288669-46tkedw7	6	44	mutant	mutant	JJ
cord-288669-46tkedw7	6	45	genomic	genomic	NN
cord-288669-46tkedw7	6	46	clone	clone	NN
cord-288669-46tkedw7	6	47	was	be	VBD
cord-288669-46tkedw7	6	48	unable	unable	JJ
cord-288669-46tkedw7	6	49	to	to	TO
cord-288669-46tkedw7	6	50	synthesize	synthesize	VB
cord-288669-46tkedw7	6	51	the	the	DT
cord-288669-46tkedw7	6	52	E	e	NN
cord-288669-46tkedw7	6	53	protein	protein	NN
cord-288669-46tkedw7	6	54	.	.	.
cord-288669-46tkedw7	7	1	DNA	dna	NN
cord-288669-46tkedw7	7	2	transfection	transfection	NN
cord-288669-46tkedw7	7	3	of	of	IN
cord-288669-46tkedw7	7	4	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	7	5	-	-	HYPH
cord-288669-46tkedw7	7	6	susceptible	susceptible	JJ
cord-288669-46tkedw7	7	7	cells	cell	NNS
cord-288669-46tkedw7	7	8	with	with	IN
cord-288669-46tkedw7	7	9	the	the	DT
cord-288669-46tkedw7	7	10	E	e	NN
cord-288669-46tkedw7	7	11	gene	gene	NN
cord-288669-46tkedw7	8	1	knocked	knock	VBN
cord-288669-46tkedw7	8	2	-	-	HYPH
cord-288669-46tkedw7	8	3	out	out	RP
cord-288669-46tkedw7	8	4	genomic	genomic	JJ
cord-288669-46tkedw7	8	5	clone	clone	NN
cord-288669-46tkedw7	8	6	showed	show	VBD
cord-288669-46tkedw7	8	7	the	the	DT
cord-288669-46tkedw7	8	8	absence	absence	NN
cord-288669-46tkedw7	8	9	of	of	IN
cord-288669-46tkedw7	8	10	virus	virus	NN
cord-288669-46tkedw7	8	11	infectivity	infectivity	NN
cord-288669-46tkedw7	8	12	.	.	.
cord-288669-46tkedw7	9	1	P129-ΔE	p129-δe	LS
cord-288669-46tkedw7	9	2	-	-	HYPH
cord-288669-46tkedw7	9	3	transfected	transfecte	VBN
cord-288669-46tkedw7	9	4	cells	cell	NNS
cord-288669-46tkedw7	9	5	however	however	RB
cord-288669-46tkedw7	9	6	produced	produce	VBD
cord-288669-46tkedw7	9	7	virion	virion	NN
cord-288669-46tkedw7	9	8	particles	particle	NNS
cord-288669-46tkedw7	9	9	in	in	IN
cord-288669-46tkedw7	9	10	the	the	DT
cord-288669-46tkedw7	9	11	culture	culture	NN
cord-288669-46tkedw7	9	12	supernatant	supernatant	NN
cord-288669-46tkedw7	9	13	,	,	,
cord-288669-46tkedw7	9	14	and	and	CC
cord-288669-46tkedw7	9	15	these	these	DT
cord-288669-46tkedw7	9	16	particles	particle	NNS
cord-288669-46tkedw7	9	17	contained	contain	VBD
cord-288669-46tkedw7	9	18	viral	viral	JJ
cord-288669-46tkedw7	9	19	genomic	genomic	JJ
cord-288669-46tkedw7	9	20	RNA	RNA	NNP
cord-288669-46tkedw7	9	21	,	,	,
cord-288669-46tkedw7	9	22	demonstrating	demonstrate	VBG
cord-288669-46tkedw7	9	23	that	that	IN
cord-288669-46tkedw7	9	24	the	the	DT
cord-288669-46tkedw7	9	25	E	e	NN
cord-288669-46tkedw7	9	26	protein	protein	NN
cord-288669-46tkedw7	9	27	is	be	VBZ
cord-288669-46tkedw7	9	28	essential	essential	JJ
cord-288669-46tkedw7	9	29	for	for	IN
cord-288669-46tkedw7	9	30	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	9	31	infection	infection	NN
cord-288669-46tkedw7	9	32	but	but	CC
cord-288669-46tkedw7	9	33	dispensable	dispensable	JJ
cord-288669-46tkedw7	9	34	for	for	IN
cord-288669-46tkedw7	9	35	virion	virion	NN
cord-288669-46tkedw7	9	36	assembly	assembly	NN
cord-288669-46tkedw7	9	37	.	.	.
cord-288669-46tkedw7	10	1	Electron	electron	NN
cord-288669-46tkedw7	10	2	microscopy	microscopy	NN
cord-288669-46tkedw7	10	3	suggests	suggest	VBZ
cord-288669-46tkedw7	10	4	that	that	IN
cord-288669-46tkedw7	10	5	the	the	DT
cord-288669-46tkedw7	10	6	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	10	7	virions	virion	NNS
cord-288669-46tkedw7	10	8	assembled	assemble	VBN
cord-288669-46tkedw7	10	9	in	in	IN
cord-288669-46tkedw7	10	10	the	the	DT
cord-288669-46tkedw7	10	11	absence	absence	NN
cord-288669-46tkedw7	10	12	of	of	IN
cord-288669-46tkedw7	10	13	E	E	NNP
cord-288669-46tkedw7	10	14	had	have	VBD
cord-288669-46tkedw7	10	15	a	a	DT
cord-288669-46tkedw7	10	16	similar	similar	JJ
cord-288669-46tkedw7	10	17	appearance	appearance	NN
cord-288669-46tkedw7	10	18	to	to	IN
cord-288669-46tkedw7	10	19	the	the	DT
cord-288669-46tkedw7	10	20	wild	wild	JJ
cord-288669-46tkedw7	10	21	-	-	HYPH
cord-288669-46tkedw7	10	22	type	type	NN
cord-288669-46tkedw7	10	23	particles	particle	NNS
cord-288669-46tkedw7	10	24	.	.	.
cord-288669-46tkedw7	11	1	Strand	strand	NN
cord-288669-46tkedw7	11	2	-	-	HYPH
cord-288669-46tkedw7	11	3	specific	specific	JJ
cord-288669-46tkedw7	11	4	RT	RT	NNP
cord-288669-46tkedw7	11	5	-	-	HYPH
cord-288669-46tkedw7	11	6	PCR	PCR	NNP
cord-288669-46tkedw7	11	7	demonstrated	demonstrate	VBD
cord-288669-46tkedw7	11	8	that	that	IN
cord-288669-46tkedw7	11	9	the	the	DT
cord-288669-46tkedw7	11	10	E	e	NN
cord-288669-46tkedw7	11	11	protein	protein	NN
cord-288669-46tkedw7	11	12	-	-	HYPH
cord-288669-46tkedw7	11	13	negative	negative	JJ
cord-288669-46tkedw7	11	14	,	,	,
cord-288669-46tkedw7	11	15	non	non	JJ
cord-288669-46tkedw7	11	16	-	-	JJ
cord-288669-46tkedw7	11	17	infectious	infectious	JJ
cord-288669-46tkedw7	11	18	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	11	19	virus	virus	NN
cord-288669-46tkedw7	11	20	particles	particle	NNS
cord-288669-46tkedw7	11	21	were	be	VBD
cord-288669-46tkedw7	11	22	able	able	JJ
cord-288669-46tkedw7	11	23	to	to	TO
cord-288669-46tkedw7	11	24	enter	enter	VB
cord-288669-46tkedw7	11	25	cells	cell	NNS
cord-288669-46tkedw7	11	26	but	but	CC
cord-288669-46tkedw7	11	27	further	further	JJ
cord-288669-46tkedw7	11	28	steps	step	NNS
cord-288669-46tkedw7	11	29	of	of	IN
cord-288669-46tkedw7	11	30	replication	replication	NN
cord-288669-46tkedw7	11	31	were	be	VBD
cord-288669-46tkedw7	11	32	interrupted	interrupt	VBN
cord-288669-46tkedw7	11	33	.	.	.
cord-288669-46tkedw7	12	1	The	the	DT
cord-288669-46tkedw7	12	2	entry	entry	NN
cord-288669-46tkedw7	12	3	of	of	IN
cord-288669-46tkedw7	12	4	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	12	5	has	have	VBZ
cord-288669-46tkedw7	12	6	been	be	VBN
cord-288669-46tkedw7	12	7	suggested	suggest	VBN
cord-288669-46tkedw7	12	8	to	to	TO
cord-288669-46tkedw7	12	9	be	be	VB
cord-288669-46tkedw7	12	10	via	via	IN
cord-288669-46tkedw7	12	11	receptor	receptor	NN
cord-288669-46tkedw7	12	12	-	-	HYPH
cord-288669-46tkedw7	12	13	mediated	mediate	VBN
cord-288669-46tkedw7	12	14	endocytosis	endocytosis	NN
cord-288669-46tkedw7	12	15	,	,	,
cord-288669-46tkedw7	12	16	and	and	CC
cord-288669-46tkedw7	12	17	lysomotropic	lysomotropic	NNP
cord-288669-46tkedw7	12	18	basic	basic	JJ
cord-288669-46tkedw7	12	19	compounds	compound	NNS
cord-288669-46tkedw7	12	20	and	and	CC
cord-288669-46tkedw7	12	21	known	know	VBN
cord-288669-46tkedw7	12	22	ion	ion	NN
cord-288669-46tkedw7	12	23	-	-	HYPH
cord-288669-46tkedw7	12	24	channel	channel	NN
cord-288669-46tkedw7	12	25	blocking	blocking	NN
cord-288669-46tkedw7	12	26	agents	agent	NNS
cord-288669-46tkedw7	12	27	both	both	CC
cord-288669-46tkedw7	12	28	inhibited	inhibit	VBN
cord-288669-46tkedw7	12	29	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	12	30	replication	replication	NN
cord-288669-46tkedw7	12	31	effectively	effectively	RB
cord-288669-46tkedw7	12	32	during	during	IN
cord-288669-46tkedw7	12	33	the	the	DT
cord-288669-46tkedw7	12	34	uncoating	uncoating	NN
cord-288669-46tkedw7	12	35	process	process	NN
cord-288669-46tkedw7	12	36	.	.	.
cord-288669-46tkedw7	13	1	The	the	DT
cord-288669-46tkedw7	13	2	expression	expression	NN
cord-288669-46tkedw7	13	3	of	of	IN
cord-288669-46tkedw7	13	4	E	e	NN
cord-288669-46tkedw7	13	5	protein	protein	NN
cord-288669-46tkedw7	13	6	in	in	IN
cord-288669-46tkedw7	13	7	Escherichia	Escherichia	NNP
cord-288669-46tkedw7	13	8	coli	coli	NNS
cord-288669-46tkedw7	13	9	-	-	HYPH
cord-288669-46tkedw7	13	10	mediated	mediate	VBN
cord-288669-46tkedw7	13	11	cell	cell	NN
cord-288669-46tkedw7	13	12	growth	growth	NN
cord-288669-46tkedw7	13	13	arrests	arrest	NNS
cord-288669-46tkedw7	13	14	and	and	CC
cord-288669-46tkedw7	13	15	increased	increase	VBD
cord-288669-46tkedw7	13	16	the	the	DT
cord-288669-46tkedw7	13	17	membrane	membrane	NN
cord-288669-46tkedw7	13	18	permeability	permeability	NN
cord-288669-46tkedw7	13	19	.	.	.
cord-288669-46tkedw7	14	1	Cross	cross	NN
cord-288669-46tkedw7	14	2	-	-	JJ
cord-288669-46tkedw7	14	3	linking	link	VBG
cord-288669-46tkedw7	14	4	experiments	experiment	NNS
cord-288669-46tkedw7	14	5	in	in	IN
cord-288669-46tkedw7	14	6	cells	cell	NNS
cord-288669-46tkedw7	14	7	infected	infect	VBN
cord-288669-46tkedw7	14	8	with	with	IN
cord-288669-46tkedw7	14	9	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	14	10	or	or	CC
cord-288669-46tkedw7	14	11	transfected	transfecte	VBN
cord-288669-46tkedw7	14	12	with	with	IN
cord-288669-46tkedw7	14	13	E	e	NN
cord-288669-46tkedw7	14	14	gene	gene	NN
cord-288669-46tkedw7	14	15	showed	show	VBD
cord-288669-46tkedw7	14	16	that	that	IN
cord-288669-46tkedw7	14	17	the	the	DT
cord-288669-46tkedw7	14	18	E	e	NN
cord-288669-46tkedw7	14	19	protein	protein	NN
cord-288669-46tkedw7	14	20	was	be	VBD
cord-288669-46tkedw7	14	21	able	able	JJ
cord-288669-46tkedw7	14	22	to	to	TO
cord-288669-46tkedw7	14	23	form	form	VB
cord-288669-46tkedw7	14	24	homo	homo	NN
cord-288669-46tkedw7	14	25	-	-	HYPH
cord-288669-46tkedw7	14	26	oligomers	oligomer	NNS
cord-288669-46tkedw7	14	27	.	.	.
cord-288669-46tkedw7	15	1	Taken	take	VBN
cord-288669-46tkedw7	15	2	together	together	RB
cord-288669-46tkedw7	15	3	,	,	,
cord-288669-46tkedw7	15	4	our	-PRON-	PRP$
cord-288669-46tkedw7	15	5	data	datum	NNS
cord-288669-46tkedw7	15	6	suggest	suggest	VBP
cord-288669-46tkedw7	15	7	that	that	IN
cord-288669-46tkedw7	15	8	the	the	DT
cord-288669-46tkedw7	15	9	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	15	10	E	E	NNP
cord-288669-46tkedw7	15	11	protein	protein	NN
cord-288669-46tkedw7	15	12	is	be	VBZ
cord-288669-46tkedw7	15	13	likely	likely	RB
cord-288669-46tkedw7	15	14	an	an	DT
cord-288669-46tkedw7	15	15	ion	ion	NN
cord-288669-46tkedw7	15	16	-	-	HYPH
cord-288669-46tkedw7	15	17	channel	channel	NN
cord-288669-46tkedw7	15	18	protein	protein	NN
cord-288669-46tkedw7	15	19	embedded	embed	VBN
cord-288669-46tkedw7	15	20	in	in	IN
cord-288669-46tkedw7	15	21	the	the	DT
cord-288669-46tkedw7	15	22	viral	viral	JJ
cord-288669-46tkedw7	15	23	envelope	envelope	NN
cord-288669-46tkedw7	15	24	and	and	CC
cord-288669-46tkedw7	15	25	facilitates	facilitate	VBZ
cord-288669-46tkedw7	15	26	uncoating	uncoating	NN
cord-288669-46tkedw7	15	27	of	of	IN
cord-288669-46tkedw7	15	28	virus	virus	NN
cord-288669-46tkedw7	15	29	and	and	CC
cord-288669-46tkedw7	15	30	release	release	NN
cord-288669-46tkedw7	15	31	of	of	IN
cord-288669-46tkedw7	15	32	the	the	DT
cord-288669-46tkedw7	15	33	genome	genome	NN
cord-288669-46tkedw7	15	34	in	in	IN
cord-288669-46tkedw7	15	35	the	the	DT
cord-288669-46tkedw7	15	36	cytoplasm	cytoplasm	NN
cord-288669-46tkedw7	15	37	.	.	.
cord-288669-46tkedw7	16	1	Porcine	porcine	JJ
cord-288669-46tkedw7	16	2	reproductive	reproductive	JJ
cord-288669-46tkedw7	16	3	and	and	CC
cord-288669-46tkedw7	16	4	respiratory	respiratory	JJ
cord-288669-46tkedw7	16	5	syndrome	syndrome	NN
cord-288669-46tkedw7	16	6	virus	virus	NN
cord-288669-46tkedw7	16	7	(	(	-LRB-
cord-288669-46tkedw7	16	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	16	9	)	)	-RRB-
cord-288669-46tkedw7	16	10	has	have	VBZ
cord-288669-46tkedw7	16	11	plagued	plague	VBN
cord-288669-46tkedw7	16	12	the	the	DT
cord-288669-46tkedw7	16	13	global	global	JJ
cord-288669-46tkedw7	16	14	swine	swine	NN
cord-288669-46tkedw7	16	15	industry	industry	NN
cord-288669-46tkedw7	16	16	leading	lead	VBG
cord-288669-46tkedw7	16	17	to	to	IN
cord-288669-46tkedw7	16	18	significant	significant	JJ
cord-288669-46tkedw7	16	19	economic	economic	JJ
cord-288669-46tkedw7	16	20	losses	loss	NNS
cord-288669-46tkedw7	16	21	for	for	IN
cord-288669-46tkedw7	16	22	pig	pig	NN
cord-288669-46tkedw7	16	23	production	production	NN
cord-288669-46tkedw7	16	24	worldwide	worldwide	RB
cord-288669-46tkedw7	17	1	(	(	-LRB-
cord-288669-46tkedw7	17	2	Nuemann	Nuemann	NNP
cord-288669-46tkedw7	17	3	et	et	NNP
cord-288669-46tkedw7	17	4	al	al	NNP
cord-288669-46tkedw7	17	5	.	.	NNP
cord-288669-46tkedw7	17	6	,	,	,
cord-288669-46tkedw7	17	7	2005	2005	CD
cord-288669-46tkedw7	17	8	)	)	-RRB-
cord-288669-46tkedw7	17	9	.	.	.
cord-288669-46tkedw7	18	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	18	2	is	be	VBZ
cord-288669-46tkedw7	18	3	a	a	DT
cord-288669-46tkedw7	18	4	member	member	NN
cord-288669-46tkedw7	18	5	of	of	IN
cord-288669-46tkedw7	18	6	the	the	DT
cord-288669-46tkedw7	18	7	family	family	NN
cord-288669-46tkedw7	18	8	Arteriviridae	Arteriviridae	NNP
cord-288669-46tkedw7	18	9	,	,	,
cord-288669-46tkedw7	18	10	which	which	WDT
cord-288669-46tkedw7	18	11	includes	include	VBZ
cord-288669-46tkedw7	18	12	equine	equine	JJ
cord-288669-46tkedw7	18	13	arteritis	arteritis	NN
cord-288669-46tkedw7	18	14	virus	virus	NN
cord-288669-46tkedw7	18	15	(	(	-LRB-
cord-288669-46tkedw7	18	16	EAV	EAV	NNP
cord-288669-46tkedw7	18	17	)	)	-RRB-
cord-288669-46tkedw7	18	18	,	,	,
cord-288669-46tkedw7	18	19	lactate	lactate	NN
cord-288669-46tkedw7	18	20	dehydrogenase	dehydrogenase	NN
cord-288669-46tkedw7	18	21	-	-	HYPH
cord-288669-46tkedw7	18	22	elevating	elevate	VBG
cord-288669-46tkedw7	18	23	virus	virus	NN
cord-288669-46tkedw7	18	24	(	(	-LRB-
cord-288669-46tkedw7	18	25	LDV	LDV	NNP
cord-288669-46tkedw7	18	26	)	)	-RRB-
cord-288669-46tkedw7	18	27	of	of	IN
cord-288669-46tkedw7	18	28	mice	mouse	NNS
cord-288669-46tkedw7	18	29	and	and	CC
cord-288669-46tkedw7	18	30	simian	simian	JJ
cord-288669-46tkedw7	18	31	hemorrhagic	hemorrhagic	JJ
cord-288669-46tkedw7	18	32	fever	fever	NN
cord-288669-46tkedw7	18	33	virus	virus	NN
cord-288669-46tkedw7	18	34	(	(	-LRB-
cord-288669-46tkedw7	18	35	SHFV	SHFV	NNP
cord-288669-46tkedw7	18	36	)	)	-RRB-
cord-288669-46tkedw7	19	1	(	(	-LRB-
cord-288669-46tkedw7	19	2	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	19	3	et	et	NNP
cord-288669-46tkedw7	19	4	al	al	NNP
cord-288669-46tkedw7	19	5	.	.	NNP
cord-288669-46tkedw7	19	6	,	,	,
cord-288669-46tkedw7	19	7	1993	1993	CD
cord-288669-46tkedw7	19	8	;	;	:
cord-288669-46tkedw7	20	1	Snijder	Snijder	NNP
cord-288669-46tkedw7	20	2	and	and	CC
cord-288669-46tkedw7	20	3	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	20	4	,	,	,
cord-288669-46tkedw7	20	5	1998	1998	CD
cord-288669-46tkedw7	20	6	)	)	-RRB-
cord-288669-46tkedw7	20	7	.	.	.
cord-288669-46tkedw7	21	1	Along	along	IN
cord-288669-46tkedw7	21	2	with	with	IN
cord-288669-46tkedw7	21	3	the	the	DT
cord-288669-46tkedw7	21	4	family	family	NN
cord-288669-46tkedw7	21	5	Coronaviridae	Coronaviridae	NNP
cord-288669-46tkedw7	21	6	,	,	,
cord-288669-46tkedw7	21	7	arteriviruses	arteriviruse	NNS
cord-288669-46tkedw7	21	8	are	be	VBP
cord-288669-46tkedw7	21	9	grouped	group	VBN
cord-288669-46tkedw7	21	10	in	in	IN
cord-288669-46tkedw7	21	11	the	the	DT
cord-288669-46tkedw7	21	12	new	new	JJ
cord-288669-46tkedw7	21	13	order	order	NN
cord-288669-46tkedw7	21	14	of	of	IN
cord-288669-46tkedw7	21	15	Nidovirales	Nidovirales	NNP
cord-288669-46tkedw7	21	16	(	(	-LRB-
cord-288669-46tkedw7	21	17	Snijder	Snijder	NNP
cord-288669-46tkedw7	21	18	et	et	NNP
cord-288669-46tkedw7	21	19	al	al	NNP
cord-288669-46tkedw7	21	20	.	.	NNP
cord-288669-46tkedw7	21	21	,	,	,
cord-288669-46tkedw7	21	22	2005	2005	CD
cord-288669-46tkedw7	21	23	)	)	-RRB-
cord-288669-46tkedw7	21	24	.	.	.
cord-288669-46tkedw7	22	1	Despite	despite	IN
cord-288669-46tkedw7	22	2	the	the	DT
cord-288669-46tkedw7	22	3	similar	similar	JJ
cord-288669-46tkedw7	22	4	virion	virion	NN
cord-288669-46tkedw7	22	5	morphology	morphology	NN
cord-288669-46tkedw7	22	6	and	and	CC
cord-288669-46tkedw7	22	7	genome	genome	NN
cord-288669-46tkedw7	22	8	organization	organization	NN
cord-288669-46tkedw7	22	9	,	,	,
cord-288669-46tkedw7	22	10	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	22	11	is	be	VBZ
cord-288669-46tkedw7	22	12	divided	divide	VBN
cord-288669-46tkedw7	22	13	into	into	IN
cord-288669-46tkedw7	22	14	two	two	CD
cord-288669-46tkedw7	22	15	genotypes	genotype	NNS
cord-288669-46tkedw7	22	16	,	,	,
cord-288669-46tkedw7	22	17	the	the	DT
cord-288669-46tkedw7	22	18	European	european	JJ
cord-288669-46tkedw7	22	19	genotype	genotype	NN
cord-288669-46tkedw7	22	20	(	(	-LRB-
cord-288669-46tkedw7	22	21	Lelystad	Lelystad	NNP
cord-288669-46tkedw7	22	22	virus	virus	NN
cord-288669-46tkedw7	22	23	;	;	:
cord-288669-46tkedw7	22	24	LV	LV	NNP
cord-288669-46tkedw7	22	25	)	)	-RRB-
cord-288669-46tkedw7	22	26	and	and	CC
cord-288669-46tkedw7	22	27	the	the	DT
cord-288669-46tkedw7	22	28	North	north	JJ
cord-288669-46tkedw7	22	29	American	american	JJ
cord-288669-46tkedw7	22	30	genotype	genotype	NN
cord-288669-46tkedw7	22	31	,	,	,
cord-288669-46tkedw7	22	32	based	base	VBN
cord-288669-46tkedw7	22	33	on	on	IN
cord-288669-46tkedw7	22	34	their	-PRON-	PRP$
cord-288669-46tkedw7	22	35	antigenic	antigenic	JJ
cord-288669-46tkedw7	22	36	and	and	CC
cord-288669-46tkedw7	22	37	genetic	genetic	JJ
cord-288669-46tkedw7	22	38	characteristics	characteristic	NNS
cord-288669-46tkedw7	22	39	(	(	-LRB-
cord-288669-46tkedw7	22	40	Meng	Meng	NNP
cord-288669-46tkedw7	22	41	et	et	FW
cord-288669-46tkedw7	22	42	al	al	NNP
cord-288669-46tkedw7	22	43	.	.	.
cord-288669-46tkedw7	23	1	,	,	,
cord-288669-46tkedw7	23	2	1995	1995	CD
cord-288669-46tkedw7	23	3	;	;	:
cord-288669-46tkedw7	23	4	Nelsen	Nelsen	NNP
cord-288669-46tkedw7	23	5	et	et	NNP
cord-288669-46tkedw7	23	6	al	al	NNP
cord-288669-46tkedw7	23	7	.	.	NNP
cord-288669-46tkedw7	23	8	,	,	,
cord-288669-46tkedw7	23	9	1999	1999	CD
cord-288669-46tkedw7	24	1	;	;	:
cord-288669-46tkedw7	24	2	Nelson	Nelson	NNP
cord-288669-46tkedw7	24	3	et	et	NNP
cord-288669-46tkedw7	24	4	al	al	NNP
cord-288669-46tkedw7	24	5	.	.	NNP
cord-288669-46tkedw7	24	6	,	,	,
cord-288669-46tkedw7	24	7	1993	1993	CD
cord-288669-46tkedw7	24	8	;	;	:
cord-288669-46tkedw7	24	9	Wootton	Wootton	NNP
cord-288669-46tkedw7	24	10	et	et	NNP
cord-288669-46tkedw7	24	11	al	al	NNP
cord-288669-46tkedw7	24	12	.	.	NNP
cord-288669-46tkedw7	24	13	,	,	,
cord-288669-46tkedw7	24	14	2000	2000	CD
cord-288669-46tkedw7	24	15	)	)	-RRB-
cord-288669-46tkedw7	24	16	.	.	.
cord-288669-46tkedw7	25	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	25	2	is	be	VBZ
cord-288669-46tkedw7	25	3	a	a	DT
cord-288669-46tkedw7	25	4	small	small	JJ
cord-288669-46tkedw7	25	5	,	,	,
cord-288669-46tkedw7	25	6	enveloped	envelop	VBN
cord-288669-46tkedw7	25	7	virus	virus	NN
cord-288669-46tkedw7	25	8	possessing	possess	VBG
cord-288669-46tkedw7	25	9	a	a	DT
cord-288669-46tkedw7	25	10	singlestranded	singlestrande	VBN
cord-288669-46tkedw7	25	11	positive	positive	JJ
cord-288669-46tkedw7	25	12	-	-	HYPH
cord-288669-46tkedw7	25	13	sense	sense	NN
cord-288669-46tkedw7	25	14	RNA	RNA	NNP
cord-288669-46tkedw7	25	15	of	of	IN
cord-288669-46tkedw7	25	16	∼	∼	NNP
cord-288669-46tkedw7	25	17	15	15	CD
cord-288669-46tkedw7	25	18	kb	kb	NNP
cord-288669-46tkedw7	25	19	in	in	IN
cord-288669-46tkedw7	25	20	size	size	NN
cord-288669-46tkedw7	25	21	with	with	IN
cord-288669-46tkedw7	25	22	a	a	DT
cord-288669-46tkedw7	25	23	5′	5′	CD
cord-288669-46tkedw7	25	24	cap	cap	NN
cord-288669-46tkedw7	25	25	and	and	CC
cord-288669-46tkedw7	25	26	a	a	DT
cord-288669-46tkedw7	25	27	3′	3′	CD
cord-288669-46tkedw7	25	28	polyadenylated	polyadenylated	JJ
cord-288669-46tkedw7	25	29	tail	tail	NN
cord-288669-46tkedw7	26	1	(	(	-LRB-
cord-288669-46tkedw7	26	2	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	26	3	et	et	NNP
cord-288669-46tkedw7	26	4	al	al	NNP
cord-288669-46tkedw7	26	5	.	.	NNP
cord-288669-46tkedw7	26	6	,	,	,
cord-288669-46tkedw7	26	7	1993	1993	CD
cord-288669-46tkedw7	26	8	;	;	:
cord-288669-46tkedw7	26	9	Sagripanti	Sagripanti	NNP
cord-288669-46tkedw7	26	10	et	et	NNP
cord-288669-46tkedw7	26	11	al	al	NNP
cord-288669-46tkedw7	26	12	.	.	NNP
cord-288669-46tkedw7	26	13	,	,	,
cord-288669-46tkedw7	26	14	1986	1986	CD
cord-288669-46tkedw7	26	15	;	;	.
cord-288669-46tkedw7	27	1	Snijder	Snijder	NNP
cord-288669-46tkedw7	27	2	and	and	CC
cord-288669-46tkedw7	27	3	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	27	4	,	,	,
cord-288669-46tkedw7	27	5	1998	1998	CD
cord-288669-46tkedw7	27	6	;	;	:
cord-288669-46tkedw7	27	7	Wootton	Wootton	NNP
cord-288669-46tkedw7	27	8	et	et	NNP
cord-288669-46tkedw7	27	9	al	al	NNP
cord-288669-46tkedw7	27	10	.	.	NNP
cord-288669-46tkedw7	27	11	,	,	,
cord-288669-46tkedw7	27	12	2000	2000	CD
cord-288669-46tkedw7	27	13	)	)	-RRB-
cord-288669-46tkedw7	27	14	.	.	.
cord-288669-46tkedw7	28	1	The	the	DT
cord-288669-46tkedw7	28	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	28	3	genome	genome	NN
cord-288669-46tkedw7	28	4	consists	consist	VBZ
cord-288669-46tkedw7	28	5	of	of	IN
cord-288669-46tkedw7	28	6	the	the	DT
cord-288669-46tkedw7	28	7	5′	5′	CD
cord-288669-46tkedw7	28	8	untranslated	untranslated	JJ
cord-288669-46tkedw7	28	9	region	region	NN
cord-288669-46tkedw7	28	10	(	(	-LRB-
cord-288669-46tkedw7	28	11	UTR	UTR	NNP
cord-288669-46tkedw7	28	12	)	)	-RRB-
cord-288669-46tkedw7	28	13	,	,	,
cord-288669-46tkedw7	28	14	nine	nine	CD
cord-288669-46tkedw7	28	15	open	open	JJ
cord-288669-46tkedw7	28	16	reading	reading	NN
cord-288669-46tkedw7	28	17	frames	frame	NNS
cord-288669-46tkedw7	28	18	(	(	-LRB-
cord-288669-46tkedw7	28	19	ORF1a	ORF1a	NNP
cord-288669-46tkedw7	28	20	,	,	,
cord-288669-46tkedw7	28	21	ORF1b	ORF1b	NNP
cord-288669-46tkedw7	28	22	,	,	,
cord-288669-46tkedw7	28	23	ORF2a	ORF2a	NNP
cord-288669-46tkedw7	28	24	,	,	,
cord-288669-46tkedw7	28	25	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	28	26	and	and	CC
cord-288669-46tkedw7	28	27	ORFs	ORFs	NNP
cord-288669-46tkedw7	28	28	3	3	CD
cord-288669-46tkedw7	28	29	through	through	IN
cord-288669-46tkedw7	28	30	7	7	CD
cord-288669-46tkedw7	28	31	)	)	-RRB-
cord-288669-46tkedw7	28	32	and	and	CC
cord-288669-46tkedw7	28	33	the	the	DT
cord-288669-46tkedw7	28	34	3′	3′	CD
cord-288669-46tkedw7	28	35	UTR	UTR	NNP
cord-288669-46tkedw7	28	36	(	(	-LRB-
cord-288669-46tkedw7	28	37	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	28	38	et	et	NNP
cord-288669-46tkedw7	28	39	al	al	NNP
cord-288669-46tkedw7	28	40	.	.	NNP
cord-288669-46tkedw7	28	41	,	,	,
cord-288669-46tkedw7	28	42	1993	1993	CD
cord-288669-46tkedw7	28	43	;	;	:
cord-288669-46tkedw7	28	44	Snijder	Snijder	NNP
cord-288669-46tkedw7	28	45	and	and	CC
cord-288669-46tkedw7	28	46	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	28	47	,	,	,
cord-288669-46tkedw7	28	48	1998	1998	CD
cord-288669-46tkedw7	28	49	;	;	:
cord-288669-46tkedw7	28	50	Wootton	Wootton	NNP
cord-288669-46tkedw7	28	51	et	et	NNP
cord-288669-46tkedw7	28	52	al	al	NNP
cord-288669-46tkedw7	28	53	.	.	NNP
cord-288669-46tkedw7	28	54	,	,	,
cord-288669-46tkedw7	28	55	2000	2000	CD
cord-288669-46tkedw7	28	56	)	)	-RRB-
cord-288669-46tkedw7	28	57	.	.	.
cord-288669-46tkedw7	29	1	Two	two	CD
cord-288669-46tkedw7	29	2	large	large	JJ
cord-288669-46tkedw7	29	3	ORFs	ORFs	NNP
cord-288669-46tkedw7	29	4	la	la	NN
cord-288669-46tkedw7	29	5	and	and	CC
cord-288669-46tkedw7	29	6	lb	lb	NNPS
cord-288669-46tkedw7	29	7	occupy	occupy	VB
cord-288669-46tkedw7	29	8	the	the	DT
cord-288669-46tkedw7	29	9	5′	5′	CD
cord-288669-46tkedw7	29	10	two	two	CD
cord-288669-46tkedw7	29	11	-	-	HYPH
cord-288669-46tkedw7	29	12	thirds	third	NNS
cord-288669-46tkedw7	29	13	of	of	IN
cord-288669-46tkedw7	29	14	the	the	DT
cord-288669-46tkedw7	29	15	genome	genome	NN
cord-288669-46tkedw7	29	16	and	and	CC
cord-288669-46tkedw7	29	17	encode	encode	VB
cord-288669-46tkedw7	29	18	13	13	CD
cord-288669-46tkedw7	29	19	non	non	JJ
cord-288669-46tkedw7	29	20	-	-	JJ
cord-288669-46tkedw7	29	21	structural	structural	JJ
cord-288669-46tkedw7	29	22	proteins	protein	NNS
cord-288669-46tkedw7	29	23	,	,	,
cord-288669-46tkedw7	29	24	which	which	WDT
cord-288669-46tkedw7	29	25	are	be	VBP
cord-288669-46tkedw7	29	26	suggested	suggest	VBN
cord-288669-46tkedw7	29	27	to	to	TO
cord-288669-46tkedw7	29	28	be	be	VB
cord-288669-46tkedw7	29	29	involved	involve	VBN
cord-288669-46tkedw7	29	30	in	in	IN
cord-288669-46tkedw7	29	31	the	the	DT
cord-288669-46tkedw7	29	32	genome	genome	NN
cord-288669-46tkedw7	29	33	replication	replication	NN
cord-288669-46tkedw7	29	34	and	and	CC
cord-288669-46tkedw7	29	35	transcription	transcription	NN
cord-288669-46tkedw7	29	36	(	(	-LRB-
cord-288669-46tkedw7	29	37	Bautista	Bautista	NNP
cord-288669-46tkedw7	29	38	et	et	NNP
cord-288669-46tkedw7	29	39	Virology	Virology	NNP
cord-288669-46tkedw7	29	40	355	355	CD
cord-288669-46tkedw7	29	41	(	(	-LRB-
cord-288669-46tkedw7	29	42	2006	2006	CD
cord-288669-46tkedw7	29	43	)	)	-RRB-
cord-288669-46tkedw7	29	44	30	30	CD
cord-288669-46tkedw7	29	45	-43	-43	NNP
cord-288669-46tkedw7	29	46	www.elsevier.com/locate/yviro	www.elsevier.com/locate/yviro	NNP
cord-288669-46tkedw7	29	47	al	al	NNP
cord-288669-46tkedw7	29	48	.	.	NNP
cord-288669-46tkedw7	29	49	,	,	,
cord-288669-46tkedw7	29	50	2002	2002	CD
cord-288669-46tkedw7	29	51	;	;	:
cord-288669-46tkedw7	29	52	van	van	NNP
cord-288669-46tkedw7	29	53	Dinten	Dinten	NNP
cord-288669-46tkedw7	29	54	et	et	FW
cord-288669-46tkedw7	29	55	al	al	NNP
cord-288669-46tkedw7	29	56	.	.	NNP
cord-288669-46tkedw7	29	57	,	,	,
cord-288669-46tkedw7	29	58	1999	1999	CD
cord-288669-46tkedw7	29	59	)	)	-RRB-
cord-288669-46tkedw7	29	60	.	.	.
cord-288669-46tkedw7	30	1	The	the	DT
cord-288669-46tkedw7	30	2	remaining	remain	VBG
cord-288669-46tkedw7	30	3	ORFs	orfs	NN
cord-288669-46tkedw7	30	4	,	,	,
cord-288669-46tkedw7	30	5	2a	2a	CD
cord-288669-46tkedw7	30	6	through	through	IN
cord-288669-46tkedw7	30	7	7	7	CD
cord-288669-46tkedw7	30	8	in	in	IN
cord-288669-46tkedw7	30	9	the	the	DT
cord-288669-46tkedw7	30	10	3′	3′	CD
cord-288669-46tkedw7	30	11	terminal	terminal	NN
cord-288669-46tkedw7	30	12	3	3	CD
cord-288669-46tkedw7	30	13	kb	kb	NNP
cord-288669-46tkedw7	30	14	region	region	NN
cord-288669-46tkedw7	30	15	,	,	,
cord-288669-46tkedw7	30	16	encode	encode	VB
cord-288669-46tkedw7	30	17	six	six	CD
cord-288669-46tkedw7	30	18	membraneassociated	membraneassociate	VBN
cord-288669-46tkedw7	30	19	proteins	protein	NNS
cord-288669-46tkedw7	30	20	(	(	-LRB-
cord-288669-46tkedw7	30	21	GP2	GP2	NNP
cord-288669-46tkedw7	30	22	,	,	,
cord-288669-46tkedw7	30	23	E	E	NNP
cord-288669-46tkedw7	30	24	,	,	,
cord-288669-46tkedw7	30	25	GP3	GP3	NNP
cord-288669-46tkedw7	30	26	,	,	,
cord-288669-46tkedw7	30	27	GP4	GP4	NNP
cord-288669-46tkedw7	30	28	,	,	,
cord-288669-46tkedw7	30	29	GP5	GP5	NNP
cord-288669-46tkedw7	30	30	and	and	CC
cord-288669-46tkedw7	30	31	M	M	NNP
cord-288669-46tkedw7	30	32	)	)	-RRB-
cord-288669-46tkedw7	31	1	present	present	JJ
cord-288669-46tkedw7	31	2	in	in	IN
cord-288669-46tkedw7	31	3	the	the	DT
cord-288669-46tkedw7	31	4	envelope	envelope	NN
cord-288669-46tkedw7	31	5	and	and	CC
cord-288669-46tkedw7	31	6	nucleocapsid	nucleocapsid	NN
cord-288669-46tkedw7	31	7	(	(	-LRB-
cord-288669-46tkedw7	31	8	N	N	NNP
cord-288669-46tkedw7	31	9	)	)	-RRB-
cord-288669-46tkedw7	31	10	protein	protein	NN
cord-288669-46tkedw7	32	1	(	(	-LRB-
cord-288669-46tkedw7	32	2	Meulenberg	Meulenberg	NNP
cord-288669-46tkedw7	32	3	et	et	NNP
cord-288669-46tkedw7	32	4	al	al	NNP
cord-288669-46tkedw7	32	5	.	.	.
cord-288669-46tkedw7	33	1	,	,	,
cord-288669-46tkedw7	33	2	1995	1995	CD
cord-288669-46tkedw7	33	3	;	;	:
cord-288669-46tkedw7	33	4	Wootton	Wootton	NNP
cord-288669-46tkedw7	33	5	et	et	NNP
cord-288669-46tkedw7	33	6	al	al	NNP
cord-288669-46tkedw7	33	7	.	.	.
cord-288669-46tkedw7	34	1	,	,	,
cord-288669-46tkedw7	34	2	2000	2000	CD
cord-288669-46tkedw7	34	3	)	)	-RRB-
cord-288669-46tkedw7	34	4	.	.	.
cord-288669-46tkedw7	35	1	Mature	mature	JJ
cord-288669-46tkedw7	35	2	virions	virion	NNS
cord-288669-46tkedw7	35	3	are	be	VBP
cord-288669-46tkedw7	35	4	spherical	spherical	JJ
cord-288669-46tkedw7	35	5	,	,	,
cord-288669-46tkedw7	35	6	enveloped	envelop	VBD
cord-288669-46tkedw7	35	7	particles	particle	NNS
cord-288669-46tkedw7	35	8	with	with	IN
cord-288669-46tkedw7	35	9	a	a	DT
cord-288669-46tkedw7	35	10	diameter	diameter	NN
cord-288669-46tkedw7	35	11	of	of	IN
cord-288669-46tkedw7	35	12	50	50	CD
cord-288669-46tkedw7	35	13	-	-	SYM
cord-288669-46tkedw7	35	14	65	65	CD
cord-288669-46tkedw7	35	15	nm	nm	NN
cord-288669-46tkedw7	35	16	and	and	CC
cord-288669-46tkedw7	35	17	contain	contain	VBP
cord-288669-46tkedw7	35	18	a	a	DT
cord-288669-46tkedw7	35	19	20-to	20-to	CD
cord-288669-46tkedw7	35	20	30-nm	30-nm	CD
cord-288669-46tkedw7	35	21	isometric	isometric	JJ
cord-288669-46tkedw7	35	22	core	core	NN
cord-288669-46tkedw7	35	23	structure	structure	NN
cord-288669-46tkedw7	35	24	enclosing	enclose	VBG
cord-288669-46tkedw7	35	25	the	the	DT
cord-288669-46tkedw7	35	26	genomic	genomic	JJ
cord-288669-46tkedw7	35	27	RNA	RNA	NNP
cord-288669-46tkedw7	35	28	(	(	-LRB-
cord-288669-46tkedw7	35	29	Benfield	Benfield	NNP
cord-288669-46tkedw7	35	30	et	et	NNP
cord-288669-46tkedw7	35	31	al	al	NNP
cord-288669-46tkedw7	35	32	.	.	NNP
cord-288669-46tkedw7	35	33	,	,	,
cord-288669-46tkedw7	35	34	1992	1992	CD
cord-288669-46tkedw7	35	35	;	;	:
cord-288669-46tkedw7	35	36	Dea	Dea	NNP
cord-288669-46tkedw7	35	37	et	et	FW
cord-288669-46tkedw7	35	38	al	al	NNP
cord-288669-46tkedw7	35	39	.	.	NNP
cord-288669-46tkedw7	35	40	,	,	,
cord-288669-46tkedw7	35	41	1995	1995	CD
cord-288669-46tkedw7	35	42	)	)	-RRB-
cord-288669-46tkedw7	35	43	.	.	.
cord-288669-46tkedw7	36	1	The	the	DT
cord-288669-46tkedw7	36	2	small	small	JJ
cord-288669-46tkedw7	36	3	envelope	envelope	NN
cord-288669-46tkedw7	36	4	(	(	-LRB-
cord-288669-46tkedw7	36	5	E	e	NN
cord-288669-46tkedw7	36	6	)	)	-RRB-
cord-288669-46tkedw7	36	7	protein	protein	NN
cord-288669-46tkedw7	36	8	is	be	VBZ
cord-288669-46tkedw7	36	9	a	a	DT
cord-288669-46tkedw7	36	10	newly	newly	RB
cord-288669-46tkedw7	36	11	identified	identify	VBN
cord-288669-46tkedw7	36	12	structural	structural	JJ
cord-288669-46tkedw7	36	13	component	component	NN
cord-288669-46tkedw7	36	14	in	in	IN
cord-288669-46tkedw7	36	15	arteriviruses	arteriviruse	NNS
cord-288669-46tkedw7	36	16	.	.	.
cord-288669-46tkedw7	37	1	The	the	DT
cord-288669-46tkedw7	37	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	37	3	E	E	NNP
cord-288669-46tkedw7	37	4	protein	protein	NN
cord-288669-46tkedw7	37	5	,	,	,
cord-288669-46tkedw7	37	6	also	also	RB
cord-288669-46tkedw7	37	7	known	know	VBN
cord-288669-46tkedw7	37	8	as	as	IN
cord-288669-46tkedw7	37	9	2b	2b	NNP
cord-288669-46tkedw7	37	10	protein	protein	NNP
cord-288669-46tkedw7	37	11	,	,	,
cord-288669-46tkedw7	37	12	is	be	VBZ
cord-288669-46tkedw7	37	13	translated	translate	VBN
cord-288669-46tkedw7	37	14	from	from	IN
cord-288669-46tkedw7	37	15	the	the	DT
cord-288669-46tkedw7	37	16	internal	internal	JJ
cord-288669-46tkedw7	37	17	ORF	ORF	NNP
cord-288669-46tkedw7	37	18	(	(	-LRB-
cord-288669-46tkedw7	37	19	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	37	20	)	)	-RRB-
cord-288669-46tkedw7	37	21	starting	start	VBG
cord-288669-46tkedw7	37	22	from	from	IN
cord-288669-46tkedw7	37	23	the	the	DT
cord-288669-46tkedw7	37	24	+	+	SYM
cord-288669-46tkedw7	37	25	6	6	CD
cord-288669-46tkedw7	37	26	nucleotide	nucleotide	JJ
cord-288669-46tkedw7	37	27	position	position	NN
cord-288669-46tkedw7	37	28	in	in	IN
cord-288669-46tkedw7	37	29	mRNA2	mrna2	NN
cord-288669-46tkedw7	38	1	(	(	-LRB-
cord-288669-46tkedw7	38	2	Fig	Fig	NNP
cord-288669-46tkedw7	38	3	.	.	.
cord-288669-46tkedw7	38	4	1A	1a	NN
cord-288669-46tkedw7	38	5	)	)	-RRB-
cord-288669-46tkedw7	38	6	.	.	.
cord-288669-46tkedw7	39	1	The	the	DT
cord-288669-46tkedw7	39	2	E	e	NN
cord-288669-46tkedw7	39	3	protein	protein	NN
cord-288669-46tkedw7	39	4	is	be	VBZ
cord-288669-46tkedw7	39	5	73	73	CD
cord-288669-46tkedw7	39	6	and	and	CC
cord-288669-46tkedw7	39	7	70	70	CD
cord-288669-46tkedw7	39	8	amino	amino	NN
cord-288669-46tkedw7	39	9	acids	acid	NNS
cord-288669-46tkedw7	39	10	for	for	IN
cord-288669-46tkedw7	39	11	the	the	DT
cord-288669-46tkedw7	39	12	North	north	JJ
cord-288669-46tkedw7	39	13	American	american	JJ
cord-288669-46tkedw7	39	14	and	and	CC
cord-288669-46tkedw7	39	15	European	european	JJ
cord-288669-46tkedw7	39	16	type	type	NN
cord-288669-46tkedw7	39	17	of	of	IN
cord-288669-46tkedw7	39	18	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	39	19	,	,	,
cord-288669-46tkedw7	39	20	respectively	respectively	RB
cord-288669-46tkedw7	39	21	.	.	.
cord-288669-46tkedw7	40	1	The	the	DT
cord-288669-46tkedw7	40	2	E	e	NN
cord-288669-46tkedw7	40	3	protein	protein	NN
cord-288669-46tkedw7	40	4	is	be	VBZ
cord-288669-46tkedw7	40	5	highly	highly	RB
cord-288669-46tkedw7	40	6	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	40	7	but	but	CC
cord-288669-46tkedw7	40	8	contains	contain	VBZ
cord-288669-46tkedw7	40	9	a	a	DT
cord-288669-46tkedw7	40	10	cluster	cluster	NN
cord-288669-46tkedw7	40	11	of	of	IN
cord-288669-46tkedw7	40	12	basic	basic	JJ
cord-288669-46tkedw7	40	13	amino	amino	NN
cord-288669-46tkedw7	40	14	acids	acid	NNS
cord-288669-46tkedw7	40	15	in	in	IN
cord-288669-46tkedw7	40	16	the	the	DT
cord-288669-46tkedw7	40	17	hydrophilic	hydrophilic	JJ
cord-288669-46tkedw7	40	18	C	c	NN
cord-288669-46tkedw7	40	19	-	-	HYPH
cord-288669-46tkedw7	40	20	terminal	terminal	NN
cord-288669-46tkedw7	40	21	region	region	NN
cord-288669-46tkedw7	40	22	.	.	.
cord-288669-46tkedw7	41	1	The	the	DT
cord-288669-46tkedw7	41	2	E	e	NN
cord-288669-46tkedw7	41	3	protein	protein	NN
cord-288669-46tkedw7	41	4	is	be	VBZ
cord-288669-46tkedw7	41	5	non	non	JJ
cord-288669-46tkedw7	41	6	-	-	JJ
cord-288669-46tkedw7	41	7	glycosylated	glycosylated	JJ
cord-288669-46tkedw7	41	8	and	and	CC
cord-288669-46tkedw7	41	9	intracellular	intracellular	JJ
cord-288669-46tkedw7	41	10	membrane	membrane	NN
cord-288669-46tkedw7	41	11	-	-	HYPH
cord-288669-46tkedw7	41	12	associated	associate	VBN
cord-288669-46tkedw7	41	13	Wu	Wu	NNP
cord-288669-46tkedw7	41	14	et	et	NNP
cord-288669-46tkedw7	41	15	al	al	NNP
cord-288669-46tkedw7	41	16	.	.	NNP
cord-288669-46tkedw7	41	17	,	,	,
cord-288669-46tkedw7	41	18	2001	2001	CD
cord-288669-46tkedw7	41	19	)	)	-RRB-
cord-288669-46tkedw7	41	20	.	.	.
cord-288669-46tkedw7	42	1	In	in	IN
cord-288669-46tkedw7	42	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	42	3	-	-	HYPH
cord-288669-46tkedw7	42	4	infected	infect	VBN
cord-288669-46tkedw7	42	5	pigs	pig	NNS
cord-288669-46tkedw7	42	6	,	,	,
cord-288669-46tkedw7	42	7	the	the	DT
cord-288669-46tkedw7	42	8	E	e	NN
cord-288669-46tkedw7	42	9	protein	protein	NN
cord-288669-46tkedw7	42	10	induces	induce	VBZ
cord-288669-46tkedw7	42	11	specific	specific	JJ
cord-288669-46tkedw7	42	12	antibody	antibody	NN
cord-288669-46tkedw7	42	13	(	(	-LRB-
cord-288669-46tkedw7	42	14	Wu	Wu	NNP
cord-288669-46tkedw7	42	15	et	et	NNP
cord-288669-46tkedw7	42	16	al	al	NNP
cord-288669-46tkedw7	42	17	.	.	NNP
cord-288669-46tkedw7	42	18	,	,	,
cord-288669-46tkedw7	42	19	2001	2001	CD
cord-288669-46tkedw7	42	20	)	)	-RRB-
cord-288669-46tkedw7	42	21	.	.	.
cord-288669-46tkedw7	43	1	Recent	recent	JJ
cord-288669-46tkedw7	43	2	studies	study	NNS
cord-288669-46tkedw7	43	3	with	with	IN
cord-288669-46tkedw7	43	4	a	a	DT
cord-288669-46tkedw7	43	5	European	european	JJ
cord-288669-46tkedw7	43	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	43	7	isolate	isolate	NN
cord-288669-46tkedw7	43	8	showed	show	VBD
cord-288669-46tkedw7	43	9	that	that	IN
cord-288669-46tkedw7	43	10	the	the	DT
cord-288669-46tkedw7	43	11	E	e	NN
cord-288669-46tkedw7	43	12	protein	protein	NN
cord-288669-46tkedw7	43	13	is	be	VBZ
cord-288669-46tkedw7	43	14	incorporated	incorporate	VBN
cord-288669-46tkedw7	43	15	into	into	IN
cord-288669-46tkedw7	43	16	the	the	DT
cord-288669-46tkedw7	43	17	virions	virion	NNS
cord-288669-46tkedw7	43	18	in	in	IN
cord-288669-46tkedw7	43	19	association	association	NN
cord-288669-46tkedw7	43	20	with	with	IN
cord-288669-46tkedw7	43	21	GP2-GP3-GP4	GP2-GP3-GP4	NNP
cord-288669-46tkedw7	43	22	heterotrimers	heterotrimers	NNP
cord-288669-46tkedw7	43	23	,	,	,
cord-288669-46tkedw7	43	24	suggesting	suggest	VBG
cord-288669-46tkedw7	43	25	a	a	DT
cord-288669-46tkedw7	43	26	critical	critical	JJ
cord-288669-46tkedw7	43	27	role	role	NN
cord-288669-46tkedw7	43	28	for	for	IN
cord-288669-46tkedw7	43	29	the	the	DT
cord-288669-46tkedw7	43	30	heteromultimeric	heteromultimeric	JJ
cord-288669-46tkedw7	43	31	complex	complex	NN
cord-288669-46tkedw7	43	32	in	in	IN
cord-288669-46tkedw7	43	33	the	the	DT
cord-288669-46tkedw7	43	34	virus	virus	NN
cord-288669-46tkedw7	43	35	entry	entry	NN
cord-288669-46tkedw7	43	36	process	process	NN
cord-288669-46tkedw7	43	37	(	(	-LRB-
cord-288669-46tkedw7	43	38	Wissink	Wissink	NNP
cord-288669-46tkedw7	43	39	et	et	NNP
cord-288669-46tkedw7	43	40	al	al	NNP
cord-288669-46tkedw7	43	41	.	.	NNP
cord-288669-46tkedw7	43	42	,	,	,
cord-288669-46tkedw7	43	43	2005	2005	CD
cord-288669-46tkedw7	43	44	)	)	-RRB-
cord-288669-46tkedw7	43	45	.	.	.
cord-288669-46tkedw7	44	1	Although	although	IN
cord-288669-46tkedw7	44	2	the	the	DT
cord-288669-46tkedw7	44	3	E	e	NN
cord-288669-46tkedw7	44	4	protein	protein	NN
cord-288669-46tkedw7	44	5	of	of	IN
cord-288669-46tkedw7	44	6	North	north	JJ
cord-288669-46tkedw7	44	7	American	american	JJ
cord-288669-46tkedw7	44	8	genotype	genotype	NN
cord-288669-46tkedw7	44	9	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	44	10	contains	contain	VBZ
cord-288669-46tkedw7	44	11	two	two	CD
cord-288669-46tkedw7	44	12	cysteine	cysteine	NN
cord-288669-46tkedw7	44	13	residues	residue	NNS
cord-288669-46tkedw7	44	14	at	at	IN
cord-288669-46tkedw7	44	15	positions	position	NNS
cord-288669-46tkedw7	44	16	49	49	CD
cord-288669-46tkedw7	44	17	and	and	CC
cord-288669-46tkedw7	44	18	54	54	CD
cord-288669-46tkedw7	44	19	,	,	,
cord-288669-46tkedw7	44	20	a	a	DT
cord-288669-46tkedw7	44	21	study	study	NN
cord-288669-46tkedw7	44	22	has	have	VBZ
cord-288669-46tkedw7	44	23	shown	show	VBN
cord-288669-46tkedw7	44	24	that	that	IN
cord-288669-46tkedw7	44	25	E	e	NN
cord-288669-46tkedw7	44	26	is	be	VBZ
cord-288669-46tkedw7	44	27	unable	unable	JJ
cord-288669-46tkedw7	44	28	to	to	TO
cord-288669-46tkedw7	44	29	form	form	VB
cord-288669-46tkedw7	44	30	disulfide	disulfide	RB
cord-288669-46tkedw7	44	31	-	-	HYPH
cord-288669-46tkedw7	44	32	linked	link	VBN
cord-288669-46tkedw7	44	33	homodimers	homodimer	NNS
cord-288669-46tkedw7	44	34	.	.	.
cord-288669-46tkedw7	45	1	In	in	IN
cord-288669-46tkedw7	45	2	that	that	DT
cord-288669-46tkedw7	45	3	study	study	NN
cord-288669-46tkedw7	45	4	,	,	,
cord-288669-46tkedw7	45	5	cysteine	cysteine	NN
cord-288669-46tkedw7	45	6	residues	residue	NNS
cord-288669-46tkedw7	45	7	of	of	IN
cord-288669-46tkedw7	45	8	the	the	DT
cord-288669-46tkedw7	45	9	E	e	NN
cord-288669-46tkedw7	45	10	protein	protein	NN
cord-288669-46tkedw7	45	11	were	be	VBD
cord-288669-46tkedw7	45	12	shown	show	VBN
cord-288669-46tkedw7	45	13	to	to	TO
cord-288669-46tkedw7	45	14	be	be	VB
cord-288669-46tkedw7	45	15	non	non	JJ
cord-288669-46tkedw7	45	16	-	-	JJ
cord-288669-46tkedw7	45	17	essential	essential	JJ
cord-288669-46tkedw7	45	18	for	for	IN
cord-288669-46tkedw7	45	19	virus	virus	NN
cord-288669-46tkedw7	45	20	multiplication	multiplication	NN
cord-288669-46tkedw7	45	21	.	.	.
cord-288669-46tkedw7	46	1	The	the	DT
cord-288669-46tkedw7	46	2	function	function	NN
cord-288669-46tkedw7	46	3	and	and	CC
cord-288669-46tkedw7	46	4	significance	significance	NN
cord-288669-46tkedw7	46	5	of	of	IN
cord-288669-46tkedw7	46	6	E	e	NN
cord-288669-46tkedw7	46	7	in	in	IN
cord-288669-46tkedw7	46	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	46	9	replication	replication	NN
cord-288669-46tkedw7	46	10	remain	remain	VBP
cord-288669-46tkedw7	46	11	to	to	TO
cord-288669-46tkedw7	46	12	be	be	VB
cord-288669-46tkedw7	46	13	determined	determine	VBN
cord-288669-46tkedw7	46	14	.	.	.
cord-288669-46tkedw7	47	1	In	in	IN
cord-288669-46tkedw7	47	2	the	the	DT
cord-288669-46tkedw7	47	3	present	present	JJ
cord-288669-46tkedw7	47	4	study	study	NN
cord-288669-46tkedw7	47	5	,	,	,
cord-288669-46tkedw7	47	6	we	-PRON-	PRP
cord-288669-46tkedw7	47	7	investigated	investigate	VBD
cord-288669-46tkedw7	47	8	the	the	DT
cord-288669-46tkedw7	47	9	role	role	NN
cord-288669-46tkedw7	47	10	of	of	IN
cord-288669-46tkedw7	47	11	E	e	NN
cord-288669-46tkedw7	47	12	protein	protein	NN
cord-288669-46tkedw7	47	13	during	during	IN
cord-288669-46tkedw7	47	14	PRRSv	PRRSv	NNS
cord-288669-46tkedw7	47	15	replication	replication	NN
cord-288669-46tkedw7	47	16	.	.	.
cord-288669-46tkedw7	48	1	An	an	DT
cord-288669-46tkedw7	48	2	infectious	infectious	JJ
cord-288669-46tkedw7	48	3	cDNA	cdna	NN
cord-288669-46tkedw7	48	4	clone	clone	NN
cord-288669-46tkedw7	48	5	was	be	VBD
cord-288669-46tkedw7	48	6	used	use	VBN
cord-288669-46tkedw7	48	7	to	to	TO
cord-288669-46tkedw7	48	8	generate	generate	VB
cord-288669-46tkedw7	48	9	an	an	DT
cord-288669-46tkedw7	48	10	E	e	NN
cord-288669-46tkedw7	48	11	gene	gene	NN
cord-288669-46tkedw7	48	12	-	-	HYPH
cord-288669-46tkedw7	48	13	knockout	knockout	NN
cord-288669-46tkedw7	48	14	mutant	mutant	JJ
cord-288669-46tkedw7	48	15	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	48	16	,	,	,
cord-288669-46tkedw7	48	17	and	and	CC
cord-288669-46tkedw7	48	18	we	-PRON-	PRP
cord-288669-46tkedw7	48	19	report	report	VBP
cord-288669-46tkedw7	48	20	here	here	RB
cord-288669-46tkedw7	48	21	that	that	IN
cord-288669-46tkedw7	48	22	the	the	DT
cord-288669-46tkedw7	48	23	E	e	NN
cord-288669-46tkedw7	48	24	protein	protein	NN
cord-288669-46tkedw7	48	25	is	be	VBZ
cord-288669-46tkedw7	48	26	essential	essential	JJ
cord-288669-46tkedw7	48	27	for	for	IN
cord-288669-46tkedw7	48	28	virus	virus	NN
cord-288669-46tkedw7	48	29	infectivity	infectivity	NN
cord-288669-46tkedw7	48	30	but	but	CC
cord-288669-46tkedw7	48	31	dispensable	dispensable	JJ
cord-288669-46tkedw7	48	32	for	for	IN
cord-288669-46tkedw7	48	33	virus	virus	NN
cord-288669-46tkedw7	48	34	particle	particle	NN
cord-288669-46tkedw7	48	35	formation	formation	NN
cord-288669-46tkedw7	48	36	.	.	.
cord-288669-46tkedw7	49	1	The	the	DT
cord-288669-46tkedw7	49	2	E	e	NN
cord-288669-46tkedw7	49	3	protein	protein	NN
cord-288669-46tkedw7	49	4	-	-	HYPH
cord-288669-46tkedw7	49	5	negative	negative	JJ
cord-288669-46tkedw7	49	6	,	,	,
cord-288669-46tkedw7	49	7	non	non	JJ
cord-288669-46tkedw7	49	8	-	-	JJ
cord-288669-46tkedw7	49	9	infectious	infectious	JJ
cord-288669-46tkedw7	49	10	virus	virus	NN
cord-288669-46tkedw7	49	11	particles	particle	NNS
cord-288669-46tkedw7	49	12	were	be	VBD
cord-288669-46tkedw7	49	13	able	able	JJ
cord-288669-46tkedw7	49	14	to	to	TO
cord-288669-46tkedw7	49	15	enter	enter	VB
cord-288669-46tkedw7	49	16	cells	cell	NNS
cord-288669-46tkedw7	49	17	but	but	CC
cord-288669-46tkedw7	49	18	unable	unable	JJ
cord-288669-46tkedw7	49	19	to	to	TO
cord-288669-46tkedw7	49	20	continue	continue	VB
cord-288669-46tkedw7	49	21	the	the	DT
cord-288669-46tkedw7	49	22	further	further	JJ
cord-288669-46tkedw7	49	23	steps	step	NNS
cord-288669-46tkedw7	49	24	of	of	IN
cord-288669-46tkedw7	49	25	replication	replication	NN
cord-288669-46tkedw7	49	26	.	.	.
cord-288669-46tkedw7	50	1	Furthermore	furthermore	RB
cord-288669-46tkedw7	50	2	,	,	,
cord-288669-46tkedw7	50	3	two	two	CD
cord-288669-46tkedw7	50	4	ion	ion	NN
cord-288669-46tkedw7	50	5	channel	channel	NN
cord-288669-46tkedw7	50	6	blockers	blocker	NNS
cord-288669-46tkedw7	50	7	were	be	VBD
cord-288669-46tkedw7	50	8	shown	show	VBN
cord-288669-46tkedw7	50	9	to	to	TO
cord-288669-46tkedw7	50	10	greatly	greatly	RB
cord-288669-46tkedw7	50	11	affect	affect	VB
cord-288669-46tkedw7	50	12	PRRSVreplication	prrsvreplication	NN
cord-288669-46tkedw7	50	13	at	at	IN
cord-288669-46tkedw7	50	14	early	early	JJ
cord-288669-46tkedw7	50	15	stages	stage	NNS
cord-288669-46tkedw7	50	16	of	of	IN
cord-288669-46tkedw7	50	17	infection	infection	NN
cord-288669-46tkedw7	50	18	,	,	,
cord-288669-46tkedw7	50	19	suggesting	suggest	VBG
cord-288669-46tkedw7	50	20	that	that	IN
cord-288669-46tkedw7	50	21	ion	ion	NN
cord-288669-46tkedw7	50	22	channel	channel	NN
cord-288669-46tkedw7	50	23	activity	activity	NN
cord-288669-46tkedw7	50	24	was	be	VBD
cord-288669-46tkedw7	50	25	essential	essential	JJ
cord-288669-46tkedw7	50	26	for	for	IN
cord-288669-46tkedw7	50	27	virus	virus	NN
cord-288669-46tkedw7	50	28	uncoating	uncoating	NN
cord-288669-46tkedw7	50	29	.	.	.
cord-288669-46tkedw7	51	1	We	-PRON-	PRP
cord-288669-46tkedw7	51	2	also	also	RB
cord-288669-46tkedw7	51	3	found	find	VBD
cord-288669-46tkedw7	51	4	that	that	DT
cord-288669-46tkedw7	51	5	expression	expression	NN
cord-288669-46tkedw7	51	6	of	of	IN
cord-288669-46tkedw7	51	7	the	the	DT
cord-288669-46tkedw7	51	8	E	e	NN
cord-288669-46tkedw7	51	9	protein	protein	NN
cord-288669-46tkedw7	51	10	enhanced	enhance	VBD
cord-288669-46tkedw7	51	11	membrane	membrane	NN
cord-288669-46tkedw7	51	12	permeability	permeability	NN
cord-288669-46tkedw7	51	13	of	of	IN
cord-288669-46tkedw7	51	14	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	51	15	B	B	NNP
cord-288669-46tkedw7	51	16	in	in	IN
cord-288669-46tkedw7	51	17	bacterial	bacterial	JJ
cord-288669-46tkedw7	51	18	cells	cell	NNS
cord-288669-46tkedw7	51	19	.	.	.
cord-288669-46tkedw7	52	1	Cross	cross	NN
cord-288669-46tkedw7	52	2	-	-	JJ
cord-288669-46tkedw7	52	3	linking	link	VBG
cord-288669-46tkedw7	52	4	studies	study	NNS
cord-288669-46tkedw7	52	5	revealed	reveal	VBD
cord-288669-46tkedw7	52	6	that	that	IN
cord-288669-46tkedw7	52	7	the	the	DT
cord-288669-46tkedw7	52	8	E	e	NN
cord-288669-46tkedw7	52	9	protein	protein	NN
cord-288669-46tkedw7	52	10	associated	associate	VBN
cord-288669-46tkedw7	52	11	with	with	IN
cord-288669-46tkedw7	52	12	itself	-PRON-	PRP
cord-288669-46tkedw7	52	13	into	into	IN
cord-288669-46tkedw7	52	14	higher	high	JJR
cord-288669-46tkedw7	52	15	-	-	HYPH
cord-288669-46tkedw7	52	16	order	order	NN
cord-288669-46tkedw7	52	17	structures	structure	NNS
cord-288669-46tkedw7	52	18	,	,	,
cord-288669-46tkedw7	52	19	including	include	VBG
cord-288669-46tkedw7	52	20	dimers	dimer	NNS
cord-288669-46tkedw7	52	21	,	,	,
cord-288669-46tkedw7	52	22	trimers	trimer	NNS
cord-288669-46tkedw7	52	23	and	and	CC
cord-288669-46tkedw7	52	24	tetramers	tetramer	NNS
cord-288669-46tkedw7	52	25	.	.	.
cord-288669-46tkedw7	53	1	Our	-PRON-	PRP$
cord-288669-46tkedw7	53	2	study	study	NN
cord-288669-46tkedw7	53	3	suggests	suggest	VBZ
cord-288669-46tkedw7	53	4	that	that	IN
cord-288669-46tkedw7	53	5	the	the	DT
cord-288669-46tkedw7	53	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	53	7	E	E	NNP
cord-288669-46tkedw7	53	8	protein	protein	NN
cord-288669-46tkedw7	53	9	may	may	MD
cord-288669-46tkedw7	53	10	function	function	VB
cord-288669-46tkedw7	53	11	as	as	IN
cord-288669-46tkedw7	53	12	a	a	DT
cord-288669-46tkedw7	53	13	viroporin	viroporin	NN
cord-288669-46tkedw7	53	14	in	in	IN
cord-288669-46tkedw7	53	15	the	the	DT
cord-288669-46tkedw7	53	16	virion	virion	NN
cord-288669-46tkedw7	53	17	envelope	envelope	NN
cord-288669-46tkedw7	53	18	that	that	WDT
cord-288669-46tkedw7	53	19	facilitates	facilitate	VBZ
cord-288669-46tkedw7	53	20	uncoating	uncoating	NN
cord-288669-46tkedw7	53	21	of	of	IN
cord-288669-46tkedw7	53	22	the	the	DT
cord-288669-46tkedw7	53	23	virus	virus	NN
cord-288669-46tkedw7	53	24	in	in	IN
cord-288669-46tkedw7	53	25	order	order	NN
cord-288669-46tkedw7	53	26	to	to	TO
cord-288669-46tkedw7	53	27	release	release	VB
cord-288669-46tkedw7	53	28	the	the	DT
cord-288669-46tkedw7	53	29	genomic	genomic	JJ
cord-288669-46tkedw7	53	30	RNA	RNA	NNP
cord-288669-46tkedw7	53	31	into	into	IN
cord-288669-46tkedw7	53	32	the	the	DT
cord-288669-46tkedw7	53	33	cytoplasm	cytoplasm	NN
cord-288669-46tkedw7	53	34	for	for	IN
cord-288669-46tkedw7	53	35	subsequent	subsequent	JJ
cord-288669-46tkedw7	53	36	replication	replication	NN
cord-288669-46tkedw7	53	37	.	.	.
cord-288669-46tkedw7	54	1	To	to	TO
cord-288669-46tkedw7	54	2	determine	determine	VB
cord-288669-46tkedw7	54	3	the	the	DT
cord-288669-46tkedw7	54	4	biological	biological	JJ
cord-288669-46tkedw7	54	5	significance	significance	NN
cord-288669-46tkedw7	54	6	of	of	IN
cord-288669-46tkedw7	54	7	E	e	NN
cord-288669-46tkedw7	54	8	protein	protein	NN
cord-288669-46tkedw7	54	9	during	during	IN
cord-288669-46tkedw7	54	10	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	54	11	infection	infection	NN
cord-288669-46tkedw7	54	12	,	,	,
cord-288669-46tkedw7	54	13	an	an	DT
cord-288669-46tkedw7	54	14	E	e	NN
cord-288669-46tkedw7	54	15	gene	gene	NN
cord-288669-46tkedw7	54	16	-	-	HYPH
cord-288669-46tkedw7	54	17	knockout	knockout	NN
cord-288669-46tkedw7	54	18	mutant	mutant	JJ
cord-288669-46tkedw7	54	19	virus	virus	NN
cord-288669-46tkedw7	54	20	was	be	VBD
cord-288669-46tkedw7	54	21	Fig	Fig	NNP
cord-288669-46tkedw7	54	22	.	.	NNP
cord-288669-46tkedw7	54	23	1	1	CD
cord-288669-46tkedw7	54	24	.	.	.
cord-288669-46tkedw7	55	1	(	(	-LRB-
cord-288669-46tkedw7	55	2	A	a	NN
cord-288669-46tkedw7	55	3	)	)	-RRB-
cord-288669-46tkedw7	55	4	The	the	DT
cord-288669-46tkedw7	55	5	partial	partial	JJ
cord-288669-46tkedw7	55	6	genome	genome	NN
cord-288669-46tkedw7	55	7	organization	organization	NN
cord-288669-46tkedw7	55	8	of	of	IN
cord-288669-46tkedw7	55	9	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	55	10	.	.	.
cord-288669-46tkedw7	56	1	Genomic	genomic	JJ
cord-288669-46tkedw7	56	2	locations	location	NNS
cord-288669-46tkedw7	56	3	of	of	IN
cord-288669-46tkedw7	56	4	GP2	GP2	NNP
cord-288669-46tkedw7	56	5	and	and	CC
cord-288669-46tkedw7	56	6	E	E	NNP
cord-288669-46tkedw7	56	7	genes	gene	NNS
cord-288669-46tkedw7	56	8	and	and	CC
cord-288669-46tkedw7	56	9	the	the	DT
cord-288669-46tkedw7	56	10	E	e	NN
cord-288669-46tkedw7	56	11	gene	gene	NN
cord-288669-46tkedw7	56	12	-	-	HYPH
cord-288669-46tkedw7	56	13	knockout	knockout	NN
cord-288669-46tkedw7	56	14	are	be	VBP
cord-288669-46tkedw7	56	15	illustrated	illustrate	VBN
cord-288669-46tkedw7	56	16	.	.	.
cord-288669-46tkedw7	57	1	(	(	-LRB-
cord-288669-46tkedw7	57	2	B	b	NN
cord-288669-46tkedw7	57	3	)	)	-RRB-
cord-288669-46tkedw7	57	4	Absence	absence	NN
cord-288669-46tkedw7	57	5	of	of	IN
cord-288669-46tkedw7	57	6	infectivity	infectivity	NN
cord-288669-46tkedw7	57	7	of	of	IN
cord-288669-46tkedw7	57	8	the	the	DT
cord-288669-46tkedw7	57	9	E	e	NN
cord-288669-46tkedw7	57	10	gene	gene	NN
cord-288669-46tkedw7	57	11	-	-	HYPH
cord-288669-46tkedw7	57	12	knockout	knockout	NN
cord-288669-46tkedw7	57	13	full	full	JJ
cord-288669-46tkedw7	57	14	-	-	HYPH
cord-288669-46tkedw7	57	15	length	length	NN
cord-288669-46tkedw7	57	16	clone	clone	NN
cord-288669-46tkedw7	57	17	for	for	IN
cord-288669-46tkedw7	57	18	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	57	19	,	,	,
cord-288669-46tkedw7	57	20	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	57	21	.	.	.
cord-288669-46tkedw7	58	1	Marc-145	marc-145	NN
cord-288669-46tkedw7	58	2	cells	cell	NNS
cord-288669-46tkedw7	58	3	were	be	VBD
cord-288669-46tkedw7	58	4	transfected	transfecte	VBN
cord-288669-46tkedw7	58	5	with	with	IN
cord-288669-46tkedw7	58	6	the	the	DT
cord-288669-46tkedw7	58	7	full	full	JJ
cord-288669-46tkedw7	58	8	-	-	HYPH
cord-288669-46tkedw7	58	9	length	length	NN
cord-288669-46tkedw7	58	10	cDNA	cdna	NN
cord-288669-46tkedw7	58	11	genomic	genomic	JJ
cord-288669-46tkedw7	58	12	clone	clone	NN
cord-288669-46tkedw7	58	13	of	of	IN
cord-288669-46tkedw7	58	14	P129-WT	p129-wt	CD
cord-288669-46tkedw7	58	15	or	or	CC
cord-288669-46tkedw7	58	16	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	58	17	and	and	CC
cord-288669-46tkedw7	58	18	incubated	incubate	VBN
cord-288669-46tkedw7	58	19	for	for	IN
cord-288669-46tkedw7	58	20	5	5	CD
cord-288669-46tkedw7	58	21	days	day	NNS
cord-288669-46tkedw7	58	22	.	.	.
cord-288669-46tkedw7	59	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	59	2	-	-	HYPH
cord-288669-46tkedw7	59	3	specific	specific	JJ
cord-288669-46tkedw7	59	4	CPEs	cpe	NNS
cord-288669-46tkedw7	59	5	were	be	VBD
cord-288669-46tkedw7	59	6	monitored	monitor	VBN
cord-288669-46tkedw7	59	7	daily	daily	RB
cord-288669-46tkedw7	59	8	and	and	CC
cord-288669-46tkedw7	59	9	photographed	photograph	VBD
cord-288669-46tkedw7	59	10	4	4	CD
cord-288669-46tkedw7	59	11	days	day	NNS
cord-288669-46tkedw7	59	12	post	post	NN
cord-288669-46tkedw7	59	13	-	-	JJ
cord-288669-46tkedw7	59	14	transfection	transfection	JJ
cord-288669-46tkedw7	59	15	(	(	-LRB-
cord-288669-46tkedw7	59	16	upper	upper	JJ
cord-288669-46tkedw7	59	17	panels	panel	NNS
cord-288669-46tkedw7	59	18	)	)	-RRB-
cord-288669-46tkedw7	59	19	.	.	.
cord-288669-46tkedw7	60	1	For	for	IN
cord-288669-46tkedw7	60	2	immunofluorescence	immunofluorescence	NN
cord-288669-46tkedw7	60	3	,	,	,
cord-288669-46tkedw7	60	4	cells	cell	NNS
cord-288669-46tkedw7	60	5	were	be	VBD
cord-288669-46tkedw7	60	6	fixed	fix	VBN
cord-288669-46tkedw7	60	7	with	with	IN
cord-288669-46tkedw7	60	8	cold	cold	JJ
cord-288669-46tkedw7	60	9	methanol	methanol	NN
cord-288669-46tkedw7	60	10	at	at	IN
cord-288669-46tkedw7	60	11	2	2	CD
cord-288669-46tkedw7	60	12	days	day	NNS
cord-288669-46tkedw7	60	13	post	post	NN
cord-288669-46tkedw7	60	14	-	-	JJ
cord-288669-46tkedw7	60	15	transfection	transfection	JJ
cord-288669-46tkedw7	60	16	and	and	CC
cord-288669-46tkedw7	60	17	incubated	incubate	VBN
cord-288669-46tkedw7	60	18	with	with	IN
cord-288669-46tkedw7	60	19	the	the	DT
cord-288669-46tkedw7	60	20	N	n	CD
cord-288669-46tkedw7	60	21	-	-	HYPH
cord-288669-46tkedw7	60	22	specific	specific	JJ
cord-288669-46tkedw7	60	23	MAb	MAb	NNS
cord-288669-46tkedw7	60	24	SDOW-17	sdow-17	CD
cord-288669-46tkedw7	60	25	(	(	-LRB-
cord-288669-46tkedw7	60	26	lower	low	JJR
cord-288669-46tkedw7	60	27	panels	panel	NNS
cord-288669-46tkedw7	60	28	)	)	-RRB-
cord-288669-46tkedw7	60	29	(	(	-LRB-
cord-288669-46tkedw7	60	30	magnification	magnification	NN
cord-288669-46tkedw7	60	31	20×	20×	CD
cord-288669-46tkedw7	60	32	)	)	-RRB-
cord-288669-46tkedw7	60	33	.	.	.
cord-288669-46tkedw7	61	1	(	(	-LRB-
cord-288669-46tkedw7	61	2	C	C	NNP
cord-288669-46tkedw7	61	3	)	)	-RRB-
cord-288669-46tkedw7	61	4	Double	double	JJ
cord-288669-46tkedw7	61	5	staining	staining	NN
cord-288669-46tkedw7	61	6	for	for	IN
cord-288669-46tkedw7	61	7	N	N	NNP
cord-288669-46tkedw7	61	8	(	(	-LRB-
cord-288669-46tkedw7	61	9	green	green	NNP
cord-288669-46tkedw7	61	10	)	)	-RRB-
cord-288669-46tkedw7	61	11	and	and	CC
cord-288669-46tkedw7	62	1	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	63	1	(	(	-LRB-
cord-288669-46tkedw7	63	2	red	red	JJ
cord-288669-46tkedw7	63	3	)	)	-RRB-
cord-288669-46tkedw7	63	4	proteins	protein	NNS
cord-288669-46tkedw7	63	5	for	for	IN
cord-288669-46tkedw7	63	6	P129-WT	p129-wt	CD
cord-288669-46tkedw7	63	7	(	(	-LRB-
cord-288669-46tkedw7	63	8	upper	upper	JJ
cord-288669-46tkedw7	63	9	panels	panel	NNS
cord-288669-46tkedw7	63	10	)	)	-RRB-
cord-288669-46tkedw7	63	11	or	or	CC
cord-288669-46tkedw7	63	12	P129-ΔE	P129-ΔE	``
cord-288669-46tkedw7	63	13	(	(	-LRB-
cord-288669-46tkedw7	63	14	lower	low	JJR
cord-288669-46tkedw7	63	15	panels	panel	NNS
cord-288669-46tkedw7	63	16	)	)	-RRB-
cord-288669-46tkedw7	63	17	.	.	.
cord-288669-46tkedw7	64	1	Marc-145	marc-145	NN
cord-288669-46tkedw7	64	2	cells	cell	NNS
cord-288669-46tkedw7	64	3	transfected	transfecte	VBN
cord-288669-46tkedw7	64	4	with	with	IN
cord-288669-46tkedw7	64	5	P129-WT	p129-wt	CD
cord-288669-46tkedw7	64	6	or	or	CC
cord-288669-46tkedw7	64	7	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	64	8	plasmid	plasmid	NN
cord-288669-46tkedw7	64	9	DNA	DNA	NNP
cord-288669-46tkedw7	64	10	were	be	VBD
cord-288669-46tkedw7	64	11	fixed	fix	VBN
cord-288669-46tkedw7	64	12	at	at	IN
cord-288669-46tkedw7	64	13	2	2	CD
cord-288669-46tkedw7	64	14	days	day	NNS
cord-288669-46tkedw7	64	15	post	post	NN
cord-288669-46tkedw7	64	16	-	-	JJ
cord-288669-46tkedw7	64	17	transfection	transfection	JJ
cord-288669-46tkedw7	64	18	and	and	CC
cord-288669-46tkedw7	64	19	co	co	JJ
cord-288669-46tkedw7	64	20	-	-	JJ
cord-288669-46tkedw7	64	21	stained	stain	VBN
cord-288669-46tkedw7	64	22	with	with	IN
cord-288669-46tkedw7	64	23	nsp2/3-specific	nsp2/3-specific	CD
cord-288669-46tkedw7	64	24	rabbit	rabbit	NN
cord-288669-46tkedw7	64	25	antiserum	antiserum	NN
cord-288669-46tkedw7	64	26	and	and	CC
cord-288669-46tkedw7	64	27	N	n	CD
cord-288669-46tkedw7	64	28	-	-	HYPH
cord-288669-46tkedw7	64	29	specific	specific	JJ
cord-288669-46tkedw7	64	30	MAb	MAb	NNS
cord-288669-46tkedw7	64	31	SDOW17	sdow17	XX
cord-288669-46tkedw7	64	32	.	.	.
cord-288669-46tkedw7	65	1	Yellow	Yellow	NNP
cord-288669-46tkedw7	65	2	indicates	indicate	VBZ
cord-288669-46tkedw7	65	3	merged	merge	VBN
cord-288669-46tkedw7	65	4	images	image	NNS
cord-288669-46tkedw7	65	5	where	where	WRB
cord-288669-46tkedw7	65	6	both	both	CC
cord-288669-46tkedw7	65	7	N	N	NNP
cord-288669-46tkedw7	65	8	and	and	CC
cord-288669-46tkedw7	65	9	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	65	10	are	be	VBP
cord-288669-46tkedw7	65	11	co	co	JJ
cord-288669-46tkedw7	65	12	-	-	VBN
cord-288669-46tkedw7	65	13	localized	localized	JJ
cord-288669-46tkedw7	65	14	.	.	.
cord-288669-46tkedw7	66	1	generated	generate	VBN
cord-288669-46tkedw7	66	2	using	use	VBG
cord-288669-46tkedw7	66	3	an	an	DT
cord-288669-46tkedw7	66	4	infectious	infectious	JJ
cord-288669-46tkedw7	66	5	cDNA	cdna	NN
cord-288669-46tkedw7	66	6	clone	clone	NN
cord-288669-46tkedw7	66	7	.	.	.
cord-288669-46tkedw7	67	1	The	the	DT
cord-288669-46tkedw7	67	2	start	start	NN
cord-288669-46tkedw7	67	3	codon	codon	NN
cord-288669-46tkedw7	67	4	of	of	IN
cord-288669-46tkedw7	67	5	ORF2b	orf2b	NN
cord-288669-46tkedw7	67	6	(	(	-LRB-
cord-288669-46tkedw7	67	7	E	e	NN
cord-288669-46tkedw7	67	8	gene	gene	NN
cord-288669-46tkedw7	67	9	)	)	-RRB-
cord-288669-46tkedw7	67	10	was	be	VBD
cord-288669-46tkedw7	67	11	modified	modify	VBN
cord-288669-46tkedw7	67	12	to	to	TO
cord-288669-46tkedw7	67	13	abolish	abolish	VB
cord-288669-46tkedw7	67	14	the	the	DT
cord-288669-46tkedw7	67	15	E	e	NN
cord-288669-46tkedw7	67	16	protein	protein	NN
cord-288669-46tkedw7	67	17	expression	expression	NN
cord-288669-46tkedw7	67	18	.	.	.
cord-288669-46tkedw7	68	1	With	with	IN
cord-288669-46tkedw7	68	2	the	the	DT
cord-288669-46tkedw7	68	3	shuttle	shuttle	NN
cord-288669-46tkedw7	68	4	plasmid	plasmid	NN
cord-288669-46tkedw7	68	5	by	by	IN
cord-288669-46tkedw7	68	6	site	site	NN
cord-288669-46tkedw7	68	7	-	-	HYPH
cord-288669-46tkedw7	68	8	directed	direct	VBN
cord-288669-46tkedw7	68	9	mutagenesis	mutagenesis	NN
cord-288669-46tkedw7	68	10	,	,	,
cord-288669-46tkedw7	68	11	'	'	''
cord-288669-46tkedw7	68	12	ATG	ATG	NNP
cord-288669-46tkedw7	68	13	'	'	''
cord-288669-46tkedw7	68	14	for	for	IN
cord-288669-46tkedw7	68	15	translation	translation	NN
cord-288669-46tkedw7	68	16	initiation	initiation	NN
cord-288669-46tkedw7	68	17	of	of	IN
cord-288669-46tkedw7	68	18	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	68	19	was	be	VBD
cord-288669-46tkedw7	68	20	changed	change	VBN
cord-288669-46tkedw7	68	21	to	to	IN
cord-288669-46tkedw7	68	22	'	'	``
cord-288669-46tkedw7	68	23	GTG	gtg	UH
cord-288669-46tkedw7	68	24	'	'	''
cord-288669-46tkedw7	68	25	at	at	IN
cord-288669-46tkedw7	68	26	genomic	genomic	JJ
cord-288669-46tkedw7	68	27	positions	position	NNS
cord-288669-46tkedw7	68	28	12,062	12,062	CD
cord-288669-46tkedw7	68	29	to	to	IN
cord-288669-46tkedw7	68	30	12,064	12,064	CD
cord-288669-46tkedw7	68	31	.	.	.
cord-288669-46tkedw7	69	1	This	this	DT
cord-288669-46tkedw7	69	2	mutation	mutation	NN
cord-288669-46tkedw7	69	3	did	do	VBD
cord-288669-46tkedw7	69	4	not	not	RB
cord-288669-46tkedw7	69	5	alter	alter	VB
cord-288669-46tkedw7	69	6	the	the	DT
cord-288669-46tkedw7	69	7	amino	amino	NN
cord-288669-46tkedw7	69	8	acid	acid	NN
cord-288669-46tkedw7	69	9	sequence	sequence	NN
cord-288669-46tkedw7	69	10	in	in	IN
cord-288669-46tkedw7	69	11	ORF2a	ORF2a	NNP
cord-288669-46tkedw7	69	12	encoding	encode	VBG
cord-288669-46tkedw7	69	13	GP2	GP2	NNP
cord-288669-46tkedw7	69	14	protein	protein	NN
cord-288669-46tkedw7	69	15	.	.	.
cord-288669-46tkedw7	70	1	The	the	DT
cord-288669-46tkedw7	70	2	A12062	A12062	NNP
cord-288669-46tkedw7	70	3	G	g	NN
cord-288669-46tkedw7	70	4	modification	modification	NN
cord-288669-46tkedw7	70	5	was	be	VBD
cord-288669-46tkedw7	70	6	subsequently	subsequently	RB
cord-288669-46tkedw7	70	7	introduced	introduce	VBN
cord-288669-46tkedw7	70	8	into	into	IN
cord-288669-46tkedw7	70	9	the	the	DT
cord-288669-46tkedw7	70	10	full	full	JJ
cord-288669-46tkedw7	70	11	-	-	HYPH
cord-288669-46tkedw7	70	12	length	length	NN
cord-288669-46tkedw7	70	13	genomic	genomic	JJ
cord-288669-46tkedw7	70	14	cDNA	cdna	NN
cord-288669-46tkedw7	70	15	clone	clone	NN
cord-288669-46tkedw7	70	16	by	by	IN
cord-288669-46tkedw7	70	17	subcloning	subclone	VBG
cord-288669-46tkedw7	70	18	the	the	DT
cord-288669-46tkedw7	70	19	Eco47III	Eco47III	NNP
cord-288669-46tkedw7	70	20	-	-	HYPH
cord-288669-46tkedw7	70	21	BsrGI	BsrGI	NNP
cord-288669-46tkedw7	70	22	fragment	fragment	NN
cord-288669-46tkedw7	70	23	obtained	obtain	VBN
cord-288669-46tkedw7	70	24	from	from	IN
cord-288669-46tkedw7	70	25	the	the	DT
cord-288669-46tkedw7	70	26	shuttle	shuttle	NN
cord-288669-46tkedw7	70	27	plasmid	plasmid	NN
cord-288669-46tkedw7	70	28	.	.	.
cord-288669-46tkedw7	71	1	Transformants	transformant	NNS
cord-288669-46tkedw7	71	2	were	be	VBD
cord-288669-46tkedw7	71	3	screened	screen	VBN
cord-288669-46tkedw7	71	4	by	by	IN
cord-288669-46tkedw7	71	5	restriction	restriction	NN
cord-288669-46tkedw7	71	6	patterns	pattern	NNS
cord-288669-46tkedw7	71	7	using	use	VBG
cord-288669-46tkedw7	71	8	XmaI	XmaI	NNP
cord-288669-46tkedw7	71	9	to	to	TO
cord-288669-46tkedw7	71	10	determine	determine	VB
cord-288669-46tkedw7	71	11	the	the	DT
cord-288669-46tkedw7	71	12	ligated	ligate	VBN
cord-288669-46tkedw7	71	13	clone	clone	NN
cord-288669-46tkedw7	71	14	,	,	,
cord-288669-46tkedw7	71	15	followed	follow	VBN
cord-288669-46tkedw7	71	16	by	by	IN
cord-288669-46tkedw7	71	17	nucleotides	nucleotide	NNS
cord-288669-46tkedw7	71	18	sequencing	sequence	VBG
cord-288669-46tkedw7	71	19	to	to	TO
cord-288669-46tkedw7	71	20	verify	verify	VB
cord-288669-46tkedw7	71	21	the	the	DT
cord-288669-46tkedw7	71	22	specific	specific	JJ
cord-288669-46tkedw7	71	23	modification	modification	NN
cord-288669-46tkedw7	71	24	in	in	IN
cord-288669-46tkedw7	71	25	the	the	DT
cord-288669-46tkedw7	71	26	full	full	JJ
cord-288669-46tkedw7	71	27	-	-	HYPH
cord-288669-46tkedw7	71	28	length	length	NN
cord-288669-46tkedw7	71	29	genome	genome	NN
cord-288669-46tkedw7	71	30	.	.	.
cord-288669-46tkedw7	72	1	The	the	DT
cord-288669-46tkedw7	72	2	resulting	result	VBG
cord-288669-46tkedw7	72	3	E	e	NN
cord-288669-46tkedw7	72	4	gene	gene	NN
cord-288669-46tkedw7	72	5	-	-	HYPH
cord-288669-46tkedw7	72	6	knockout	knockout	NN
cord-288669-46tkedw7	72	7	genomic	genomic	NN
cord-288669-46tkedw7	72	8	clone	clone	NN
cord-288669-46tkedw7	72	9	was	be	VBD
cord-288669-46tkedw7	72	10	designated	designate	VBN
cord-288669-46tkedw7	72	11	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	72	12	.	.	.
cord-288669-46tkedw7	73	1	The	the	DT
cord-288669-46tkedw7	73	2	infectivity	infectivity	NN
cord-288669-46tkedw7	73	3	of	of	IN
cord-288669-46tkedw7	73	4	the	the	DT
cord-288669-46tkedw7	73	5	E	e	NN
cord-288669-46tkedw7	73	6	gene	gene	NN
cord-288669-46tkedw7	73	7	-	-	HYPH
cord-288669-46tkedw7	73	8	knockout	knockout	NN
cord-288669-46tkedw7	73	9	clone	clone	NN
cord-288669-46tkedw7	73	10	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	73	11	was	be	VBD
cord-288669-46tkedw7	73	12	examined	examine	VBN
cord-288669-46tkedw7	73	13	by	by	IN
cord-288669-46tkedw7	73	14	transfection	transfection	NN
cord-288669-46tkedw7	73	15	of	of	IN
cord-288669-46tkedw7	73	16	Marc-145	marc-145	CD
cord-288669-46tkedw7	73	17	cells	cell	NNS
cord-288669-46tkedw7	73	18	.	.	.
cord-288669-46tkedw7	74	1	Cells	cell	NNS
cord-288669-46tkedw7	74	2	were	be	VBD
cord-288669-46tkedw7	74	3	transfected	transfecte	VBN
cord-288669-46tkedw7	74	4	with	with	IN
cord-288669-46tkedw7	74	5	P129-WT	p129-wt	CD
cord-288669-46tkedw7	74	6	or	or	CC
cord-288669-46tkedw7	74	7	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	74	8	,	,	,
cord-288669-46tkedw7	74	9	and	and	CC
cord-288669-46tkedw7	74	10	the	the	DT
cord-288669-46tkedw7	74	11	appearance	appearance	NN
cord-288669-46tkedw7	74	12	of	of	IN
cord-288669-46tkedw7	74	13	CPE	CPE	NNP
cord-288669-46tkedw7	74	14	was	be	VBD
cord-288669-46tkedw7	74	15	monitored	monitor	VBN
cord-288669-46tkedw7	74	16	daily	daily	RB
cord-288669-46tkedw7	74	17	.	.	.
cord-288669-46tkedw7	75	1	P129-WT	p129-wt	CD
cord-288669-46tkedw7	75	2	induced	induce	VBD
cord-288669-46tkedw7	75	3	visible	visible	JJ
cord-288669-46tkedw7	75	4	CPE	CPE	NNP
cord-288669-46tkedw7	75	5	at	at	IN
cord-288669-46tkedw7	75	6	3	3	CD
cord-288669-46tkedw7	75	7	days	day	NNS
cord-288669-46tkedw7	75	8	post	post	NN
cord-288669-46tkedw7	75	9	-	-	JJ
cord-288669-46tkedw7	75	10	transfection	transfection	JJ
cord-288669-46tkedw7	75	11	and	and	CC
cord-288669-46tkedw7	75	12	showed	show	VBD
cord-288669-46tkedw7	75	13	N	n	CD
cord-288669-46tkedw7	75	14	-	-	HYPH
cord-288669-46tkedw7	75	15	specific	specific	JJ
cord-288669-46tkedw7	75	16	staining	staining	NN
cord-288669-46tkedw7	75	17	in	in	IN
cord-288669-46tkedw7	75	18	clusters	cluster	NNS
cord-288669-46tkedw7	75	19	of	of	IN
cord-288669-46tkedw7	75	20	cells	cell	NNS
cord-288669-46tkedw7	75	21	,	,	,
cord-288669-46tkedw7	75	22	indicating	indicate	VBG
cord-288669-46tkedw7	75	23	the	the	DT
cord-288669-46tkedw7	75	24	infection	infection	NN
cord-288669-46tkedw7	75	25	and	and	CC
cord-288669-46tkedw7	75	26	spread	spread	NN
cord-288669-46tkedw7	75	27	of	of	IN
cord-288669-46tkedw7	75	28	virus	virus	NN
cord-288669-46tkedw7	75	29	to	to	IN
cord-288669-46tkedw7	75	30	neighboring	neighboring	NN
cord-288669-46tkedw7	75	31	cells	cell	NNS
cord-288669-46tkedw7	75	32	(	(	-LRB-
cord-288669-46tkedw7	75	33	Fig	fig	NN
cord-288669-46tkedw7	75	34	.	.	.
cord-288669-46tkedw7	75	35	1B	1b	CD
cord-288669-46tkedw7	75	36	,	,	,
cord-288669-46tkedw7	75	37	left	left	JJ
cord-288669-46tkedw7	75	38	panels	panel	NNS
cord-288669-46tkedw7	75	39	)	)	-RRB-
cord-288669-46tkedw7	75	40	.	.	.
cord-288669-46tkedw7	76	1	In	in	IN
cord-288669-46tkedw7	76	2	contrast	contrast	NN
cord-288669-46tkedw7	76	3	,	,	,
cord-288669-46tkedw7	76	4	transfection	transfection	NN
cord-288669-46tkedw7	76	5	of	of	IN
cord-288669-46tkedw7	76	6	P129-ΔE	P129-ΔE	NNP
cord-288669-46tkedw7	76	7	did	do	VBD
cord-288669-46tkedw7	76	8	not	not	RB
cord-288669-46tkedw7	76	9	produce	produce	VB
cord-288669-46tkedw7	76	10	any	any	DT
cord-288669-46tkedw7	76	11	visible	visible	JJ
cord-288669-46tkedw7	76	12	CPE	CPE	NNP
cord-288669-46tkedw7	76	13	for	for	IN
cord-288669-46tkedw7	76	14	up	up	IN
cord-288669-46tkedw7	76	15	to	to	IN
cord-288669-46tkedw7	76	16	at	at	RB
cord-288669-46tkedw7	76	17	least	least	RBS
cord-288669-46tkedw7	76	18	7	7	CD
cord-288669-46tkedw7	76	19	days	day	NNS
cord-288669-46tkedw7	76	20	post	post	NN
cord-288669-46tkedw7	76	21	-	-	NN
cord-288669-46tkedw7	76	22	transfection	transfection	JJ
cord-288669-46tkedw7	76	23	,	,	,
cord-288669-46tkedw7	76	24	suggesting	suggest	VBG
cord-288669-46tkedw7	76	25	the	the	DT
cord-288669-46tkedw7	76	26	lack	lack	NN
cord-288669-46tkedw7	76	27	of	of	IN
cord-288669-46tkedw7	76	28	infectivity	infectivity	NN
cord-288669-46tkedw7	76	29	.	.	.
cord-288669-46tkedw7	77	1	A	a	DT
cord-288669-46tkedw7	77	2	few	few	JJ
cord-288669-46tkedw7	77	3	single	single	JJ
cord-288669-46tkedw7	77	4	cells	cell	NNS
cord-288669-46tkedw7	77	5	exhibited	exhibit	VBD
cord-288669-46tkedw7	77	6	N	n	CD
cord-288669-46tkedw7	77	7	-	-	HYPH
cord-288669-46tkedw7	77	8	specific	specific	JJ
cord-288669-46tkedw7	77	9	fluorescence	fluorescence	NN
cord-288669-46tkedw7	77	10	,	,	,
cord-288669-46tkedw7	77	11	and	and	CC
cord-288669-46tkedw7	77	12	these	these	DT
cord-288669-46tkedw7	77	13	cells	cell	NNS
cord-288669-46tkedw7	77	14	represent	represent	VBP
cord-288669-46tkedw7	77	15	individually	individually	RB
cord-288669-46tkedw7	77	16	transfected	transfecte	VBN
cord-288669-46tkedw7	77	17	cells	cell	NNS
cord-288669-46tkedw7	77	18	with	with	IN
cord-288669-46tkedw7	77	19	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	78	1	(	(	-LRB-
cord-288669-46tkedw7	78	2	Fig	Fig	NNP
cord-288669-46tkedw7	78	3	.	.	.
cord-288669-46tkedw7	78	4	1B	1b	CD
cord-288669-46tkedw7	78	5	)	)	-RRB-
cord-288669-46tkedw7	78	6	.	.	.
cord-288669-46tkedw7	79	1	To	to	TO
cord-288669-46tkedw7	79	2	rule	rule	VB
cord-288669-46tkedw7	79	3	out	out	RP
cord-288669-46tkedw7	79	4	a	a	DT
cord-288669-46tkedw7	79	5	possibility	possibility	NN
cord-288669-46tkedw7	79	6	that	that	IN
cord-288669-46tkedw7	79	7	an	an	DT
cord-288669-46tkedw7	79	8	additional	additional	JJ
cord-288669-46tkedw7	79	9	mutation	mutation	NN
cord-288669-46tkedw7	79	10	might	may	MD
cord-288669-46tkedw7	79	11	have	have	VB
cord-288669-46tkedw7	79	12	been	be	VBN
cord-288669-46tkedw7	79	13	introduced	introduce	VBN
cord-288669-46tkedw7	79	14	during	during	IN
cord-288669-46tkedw7	79	15	construction	construction	NN
cord-288669-46tkedw7	79	16	of	of	IN
cord-288669-46tkedw7	79	17	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	79	18	,	,	,
cord-288669-46tkedw7	79	19	which	which	WDT
cord-288669-46tkedw7	79	20	may	may	MD
cord-288669-46tkedw7	79	21	have	have	VB
cord-288669-46tkedw7	79	22	resulted	result	VBN
cord-288669-46tkedw7	79	23	in	in	IN
cord-288669-46tkedw7	79	24	the	the	DT
cord-288669-46tkedw7	79	25	loss	loss	NN
cord-288669-46tkedw7	79	26	of	of	IN
cord-288669-46tkedw7	79	27	infectivity	infectivity	NN
cord-288669-46tkedw7	79	28	,	,	,
cord-288669-46tkedw7	79	29	six	six	CD
cord-288669-46tkedw7	79	30	individual	individual	JJ
cord-288669-46tkedw7	79	31	mutant	mutant	JJ
cord-288669-46tkedw7	79	32	clones	clone	NNS
cord-288669-46tkedw7	79	33	were	be	VBD
cord-288669-46tkedw7	79	34	independently	independently	RB
cord-288669-46tkedw7	79	35	generated	generate	VBN
cord-288669-46tkedw7	79	36	.	.	.
cord-288669-46tkedw7	80	1	In	in	IN
cord-288669-46tkedw7	80	2	these	these	DT
cord-288669-46tkedw7	80	3	clones	clone	NNS
cord-288669-46tkedw7	80	4	,	,	,
cord-288669-46tkedw7	80	5	no	no	DT
cord-288669-46tkedw7	80	6	mutation	mutation	NN
cord-288669-46tkedw7	80	7	was	be	VBD
cord-288669-46tkedw7	80	8	identified	identify	VBN
cord-288669-46tkedw7	80	9	for	for	IN
cord-288669-46tkedw7	80	10	the	the	DT
cord-288669-46tkedw7	80	11	ORF2	ORF2	NNP
cord-288669-46tkedw7	80	12	gene	gene	NN
cord-288669-46tkedw7	80	13	,	,	,
cord-288669-46tkedw7	80	14	and	and	CC
cord-288669-46tkedw7	80	15	upon	upon	IN
cord-288669-46tkedw7	80	16	transfection	transfection	NN
cord-288669-46tkedw7	80	17	of	of	IN
cord-288669-46tkedw7	80	18	cells	cell	NNS
cord-288669-46tkedw7	80	19	,	,	,
cord-288669-46tkedw7	80	20	all	all	DT
cord-288669-46tkedw7	80	21	six	six	CD
cord-288669-46tkedw7	80	22	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	80	23	mutant	mutant	JJ
cord-288669-46tkedw7	80	24	clones	clone	NNS
cord-288669-46tkedw7	80	25	were	be	VBD
cord-288669-46tkedw7	80	26	noninfectious	noninfectious	JJ
cord-288669-46tkedw7	80	27	(	(	-LRB-
cord-288669-46tkedw7	80	28	data	datum	NNS
cord-288669-46tkedw7	80	29	not	not	RB
cord-288669-46tkedw7	80	30	shown	show	VBN
cord-288669-46tkedw7	80	31	)	)	-RRB-
cord-288669-46tkedw7	80	32	.	.	.
cord-288669-46tkedw7	81	1	This	this	DT
cord-288669-46tkedw7	81	2	confirmed	confirm	VBD
cord-288669-46tkedw7	81	3	the	the	DT
cord-288669-46tkedw7	81	4	conclusion	conclusion	NN
cord-288669-46tkedw7	81	5	that	that	IN
cord-288669-46tkedw7	81	6	P129-ΔE	P129-ΔE	``
cord-288669-46tkedw7	81	7	was	be	VBD
cord-288669-46tkedw7	81	8	non	non	JJ
cord-288669-46tkedw7	81	9	-	-	JJ
cord-288669-46tkedw7	81	10	viable	viable	JJ
cord-288669-46tkedw7	81	11	and	and	CC
cord-288669-46tkedw7	81	12	the	the	DT
cord-288669-46tkedw7	81	13	absence	absence	NN
cord-288669-46tkedw7	81	14	of	of	IN
cord-288669-46tkedw7	81	15	infectivity	infectivity	NN
cord-288669-46tkedw7	81	16	was	be	VBD
cord-288669-46tkedw7	81	17	due	due	JJ
cord-288669-46tkedw7	81	18	to	to	IN
cord-288669-46tkedw7	81	19	the	the	DT
cord-288669-46tkedw7	81	20	lack	lack	NN
cord-288669-46tkedw7	81	21	of	of	IN
cord-288669-46tkedw7	81	22	E	e	NN
cord-288669-46tkedw7	81	23	protein	protein	NN
cord-288669-46tkedw7	81	24	expression	expression	NN
cord-288669-46tkedw7	81	25	,	,	,
cord-288669-46tkedw7	81	26	demonstrating	demonstrate	VBG
cord-288669-46tkedw7	81	27	the	the	DT
cord-288669-46tkedw7	81	28	essential	essential	JJ
cord-288669-46tkedw7	81	29	role	role	NN
cord-288669-46tkedw7	81	30	of	of	IN
cord-288669-46tkedw7	81	31	E	e	NN
cord-288669-46tkedw7	81	32	for	for	IN
cord-288669-46tkedw7	81	33	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	81	34	replication	replication	NN
cord-288669-46tkedw7	81	35	.	.	.
cord-288669-46tkedw7	82	1	The	the	DT
cord-288669-46tkedw7	82	2	transcription	transcription	NN
cord-288669-46tkedw7	82	3	ability	ability	NN
cord-288669-46tkedw7	82	4	of	of	IN
cord-288669-46tkedw7	82	5	P129-ΔE	P129-ΔE	NNP
cord-288669-46tkedw7	82	6	was	be	VBD
cord-288669-46tkedw7	82	7	examined	examine	VBN
cord-288669-46tkedw7	82	8	by	by	IN
cord-288669-46tkedw7	82	9	double	double	JJ
cord-288669-46tkedw7	82	10	staining	staining	NN
cord-288669-46tkedw7	82	11	of	of	IN
cord-288669-46tkedw7	82	12	transfected	transfecte	VBN
cord-288669-46tkedw7	82	13	cells	cell	NNS
cord-288669-46tkedw7	82	14	using	use	VBG
cord-288669-46tkedw7	82	15	the	the	DT
cord-288669-46tkedw7	82	16	Alexa	Alexa	NNP
cord-288669-46tkedw7	82	17	greenlabeled	greenlabele	VBN
cord-288669-46tkedw7	82	18	SDOW17	SDOW17	NNP
cord-288669-46tkedw7	82	19	MAb	MAb	NNS
cord-288669-46tkedw7	82	20	specific	specific	JJ
cord-288669-46tkedw7	82	21	for	for	IN
cord-288669-46tkedw7	82	22	N	N	NNP
cord-288669-46tkedw7	82	23	and	and	CC
cord-288669-46tkedw7	82	24	the	the	DT
cord-288669-46tkedw7	82	25	Texas	Texas	NNP
cord-288669-46tkedw7	82	26	red	red	NN
cord-288669-46tkedw7	82	27	-	-	HYPH
cord-288669-46tkedw7	82	28	labeled	label	VBN
cord-288669-46tkedw7	82	29	rabbit	rabbit	NN
cord-288669-46tkedw7	82	30	antiserum	antiserum	NN
cord-288669-46tkedw7	82	31	specific	specific	JJ
cord-288669-46tkedw7	82	32	for	for	IN
cord-288669-46tkedw7	82	33	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	82	34	non	non	JJ
cord-288669-46tkedw7	82	35	-	-	JJ
cord-288669-46tkedw7	82	36	structural	structural	JJ
cord-288669-46tkedw7	82	37	proteins	protein	NNS
cord-288669-46tkedw7	82	38	(	(	-LRB-
cord-288669-46tkedw7	82	39	Fig	Fig	NNP
cord-288669-46tkedw7	82	40	.	.	.
cord-288669-46tkedw7	82	41	 	 	_SP
cord-288669-46tkedw7	82	42	1C	1C	NNP
cord-288669-46tkedw7	82	43	)	)	-RRB-
cord-288669-46tkedw7	82	44	.	.	.
cord-288669-46tkedw7	83	1	In	in	IN
cord-288669-46tkedw7	83	2	cells	cell	NNS
cord-288669-46tkedw7	83	3	transfected	transfecte	VBN
cord-288669-46tkedw7	83	4	with	with	IN
cord-288669-46tkedw7	83	5	P129-WT	p129-wt	CD
cord-288669-46tkedw7	83	6	DNA	dna	NN
cord-288669-46tkedw7	83	7	,	,	,
cord-288669-46tkedw7	83	8	clusters	cluster	NNS
cord-288669-46tkedw7	83	9	of	of	IN
cord-288669-46tkedw7	83	10	cells	cell	NNS
cord-288669-46tkedw7	83	11	were	be	VBD
cord-288669-46tkedw7	83	12	stained	stain	VBN
cord-288669-46tkedw7	83	13	by	by	IN
cord-288669-46tkedw7	83	14	both	both	DT
cord-288669-46tkedw7	83	15	antibodies	antibody	NNS
cord-288669-46tkedw7	83	16	,	,	,
cord-288669-46tkedw7	83	17	indicating	indicate	VBG
cord-288669-46tkedw7	83	18	both	both	CC
cord-288669-46tkedw7	83	19	N	N	NNP
cord-288669-46tkedw7	83	20	(	(	-LRB-
cord-288669-46tkedw7	83	21	green	green	NNP
cord-288669-46tkedw7	83	22	)	)	-RRB-
cord-288669-46tkedw7	83	23	and	and	CC
cord-288669-46tkedw7	83	24	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	83	25	(	(	-LRB-
cord-288669-46tkedw7	83	26	red	red	JJ
cord-288669-46tkedw7	83	27	)	)	-RRB-
cord-288669-46tkedw7	83	28	expressions	expression	NNS
cord-288669-46tkedw7	83	29	(	(	-LRB-
cord-288669-46tkedw7	83	30	Fig	Fig	NNP
cord-288669-46tkedw7	83	31	.	.	.
cord-288669-46tkedw7	83	32	1C	1C	NNP
cord-288669-46tkedw7	83	33	,	,	,
cord-288669-46tkedw7	83	34	upper	upper	JJ
cord-288669-46tkedw7	83	35	panels	panel	NNS
cord-288669-46tkedw7	83	36	)	)	-RRB-
cord-288669-46tkedw7	83	37	.	.	.
cord-288669-46tkedw7	84	1	Merging	merge	VBG
cord-288669-46tkedw7	84	2	of	of	IN
cord-288669-46tkedw7	84	3	the	the	DT
cord-288669-46tkedw7	84	4	two	two	CD
cord-288669-46tkedw7	84	5	images	image	NNS
cord-288669-46tkedw7	84	6	showed	show	VBD
cord-288669-46tkedw7	84	7	yellow	yellow	JJ
cord-288669-46tkedw7	84	8	regions	region	NNS
cord-288669-46tkedw7	84	9	where	where	WRB
cord-288669-46tkedw7	84	10	the	the	DT
cord-288669-46tkedw7	84	11	two	two	CD
cord-288669-46tkedw7	84	12	proteins	protein	NNS
cord-288669-46tkedw7	84	13	colocalized	colocalize	VBN
cord-288669-46tkedw7	84	14	.	.	.
cord-288669-46tkedw7	85	1	For	for	IN
cord-288669-46tkedw7	85	2	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	85	3	,	,	,
cord-288669-46tkedw7	85	4	dual	dual	JJ
cord-288669-46tkedw7	85	5	-	-	HYPH
cord-288669-46tkedw7	85	6	staining	staining	NN
cord-288669-46tkedw7	85	7	of	of	IN
cord-288669-46tkedw7	85	8	N	N	NNP
cord-288669-46tkedw7	85	9	(	(	-LRB-
cord-288669-46tkedw7	85	10	green	green	NNP
cord-288669-46tkedw7	85	11	)	)	-RRB-
cord-288669-46tkedw7	86	1	and	and	CC
cord-288669-46tkedw7	86	2	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	86	3	(	(	-LRB-
cord-288669-46tkedw7	86	4	red	red	NNP
cord-288669-46tkedw7	86	5	)	)	-RRB-
cord-288669-46tkedw7	86	6	was	be	VBD
cord-288669-46tkedw7	86	7	also	also	RB
cord-288669-46tkedw7	86	8	observed	observe	VBN
cord-288669-46tkedw7	86	9	but	but	CC
cord-288669-46tkedw7	86	10	limited	limit	VBN
cord-288669-46tkedw7	86	11	to	to	IN
cord-288669-46tkedw7	86	12	transfected	transfecte	VBN
cord-288669-46tkedw7	86	13	cells	cell	NNS
cord-288669-46tkedw7	86	14	only	only	RB
cord-288669-46tkedw7	86	15	,	,	,
cord-288669-46tkedw7	86	16	and	and	CC
cord-288669-46tkedw7	86	17	no	no	DT
cord-288669-46tkedw7	86	18	evidence	evidence	NN
cord-288669-46tkedw7	86	19	for	for	IN
cord-288669-46tkedw7	86	20	the	the	DT
cord-288669-46tkedw7	86	21	spread	spread	NN
cord-288669-46tkedw7	86	22	of	of	IN
cord-288669-46tkedw7	86	23	infection	infection	NN
cord-288669-46tkedw7	86	24	was	be	VBD
cord-288669-46tkedw7	86	25	obtained	obtain	VBN
cord-288669-46tkedw7	86	26	(	(	-LRB-
cord-288669-46tkedw7	86	27	Fig	Fig	NNP
cord-288669-46tkedw7	86	28	.	.	.
cord-288669-46tkedw7	86	29	1C	1C	NNP
cord-288669-46tkedw7	86	30	,	,	,
cord-288669-46tkedw7	86	31	lower	low	JJR
cord-288669-46tkedw7	86	32	panels	panel	NNS
cord-288669-46tkedw7	86	33	)	)	-RRB-
cord-288669-46tkedw7	86	34	.	.	.
cord-288669-46tkedw7	87	1	The	the	DT
cord-288669-46tkedw7	87	2	dual	dual	JJ
cord-288669-46tkedw7	87	3	staining	staining	NN
cord-288669-46tkedw7	87	4	demonstrated	demonstrate	VBD
cord-288669-46tkedw7	87	5	the	the	DT
cord-288669-46tkedw7	87	6	expression	expression	NN
cord-288669-46tkedw7	87	7	of	of	IN
cord-288669-46tkedw7	87	8	nsp2/3	nsp2/3	NNP
cord-288669-46tkedw7	87	9	and	and	CC
cord-288669-46tkedw7	87	10	N	n	CD
cord-288669-46tkedw7	87	11	proteins	protein	NNS
cord-288669-46tkedw7	87	12	in	in	IN
cord-288669-46tkedw7	87	13	transfected	transfecte	VBN
cord-288669-46tkedw7	87	14	cells	cell	NNS
cord-288669-46tkedw7	87	15	,	,	,
cord-288669-46tkedw7	87	16	indicating	indicate	VBG
cord-288669-46tkedw7	87	17	the	the	DT
cord-288669-46tkedw7	87	18	synthesis	synthesis	NN
cord-288669-46tkedw7	87	19	of	of	IN
cord-288669-46tkedw7	87	20	both	both	DT
cord-288669-46tkedw7	87	21	non	non	JJ
cord-288669-46tkedw7	87	22	-	-	JJ
cord-288669-46tkedw7	87	23	structural	structural	JJ
cord-288669-46tkedw7	87	24	and	and	CC
cord-288669-46tkedw7	87	25	structural	structural	JJ
cord-288669-46tkedw7	87	26	proteins	protein	NNS
cord-288669-46tkedw7	87	27	,	,	,
cord-288669-46tkedw7	87	28	which	which	WDT
cord-288669-46tkedw7	87	29	in	in	IN
cord-288669-46tkedw7	87	30	turn	turn	NN
cord-288669-46tkedw7	87	31	suggests	suggest	VBZ
cord-288669-46tkedw7	87	32	that	that	IN
cord-288669-46tkedw7	87	33	the	the	DT
cord-288669-46tkedw7	87	34	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	87	35	genome	genome	NN
cord-288669-46tkedw7	87	36	replicated	replicate	VBD
cord-288669-46tkedw7	87	37	and	and	CC
cord-288669-46tkedw7	87	38	mRNA	mrna	NN
cord-288669-46tkedw7	87	39	transcription	transcription	NN
cord-288669-46tkedw7	87	40	occurred	occur	VBD
cord-288669-46tkedw7	87	41	upon	upon	IN
cord-288669-46tkedw7	87	42	transfection	transfection	NN
cord-288669-46tkedw7	87	43	of	of	IN
cord-288669-46tkedw7	87	44	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	87	45	DNA	dna	NN
cord-288669-46tkedw7	87	46	.	.	.
cord-288669-46tkedw7	88	1	The	the	DT
cord-288669-46tkedw7	88	2	results	result	NNS
cord-288669-46tkedw7	88	3	demonstrate	demonstrate	VBP
cord-288669-46tkedw7	88	4	that	that	IN
cord-288669-46tkedw7	88	5	E	e	NN
cord-288669-46tkedw7	88	6	protein	protein	NN
cord-288669-46tkedw7	88	7	expression	expression	NN
cord-288669-46tkedw7	88	8	is	be	VBZ
cord-288669-46tkedw7	88	9	required	require	VBN
cord-288669-46tkedw7	88	10	for	for	IN
cord-288669-46tkedw7	88	11	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	88	12	infectivity	infectivity	NN
cord-288669-46tkedw7	88	13	,	,	,
cord-288669-46tkedw7	88	14	but	but	CC
cord-288669-46tkedw7	88	15	genome	genome	NN
cord-288669-46tkedw7	88	16	replication	replication	NN
cord-288669-46tkedw7	88	17	and	and	CC
cord-288669-46tkedw7	88	18	transcription	transcription	NN
cord-288669-46tkedw7	88	19	may	may	MD
cord-288669-46tkedw7	88	20	occur	occur	VB
cord-288669-46tkedw7	88	21	without	without	IN
cord-288669-46tkedw7	88	22	E	e	NN
cord-288669-46tkedw7	88	23	protein	protein	NN
cord-288669-46tkedw7	88	24	.	.	.
cord-288669-46tkedw7	89	1	It	-PRON-	PRP
cord-288669-46tkedw7	89	2	leads	lead	VBZ
cord-288669-46tkedw7	89	3	us	-PRON-	PRP
cord-288669-46tkedw7	89	4	to	to	TO
cord-288669-46tkedw7	89	5	hypothesize	hypothesize	VB
cord-288669-46tkedw7	89	6	that	that	IN
cord-288669-46tkedw7	89	7	either	either	DT
cord-288669-46tkedw7	89	8	virion	virion	NN
cord-288669-46tkedw7	89	9	assembly	assembly	NN
cord-288669-46tkedw7	89	10	or	or	CC
cord-288669-46tkedw7	89	11	virus	virus	NN
cord-288669-46tkedw7	89	12	entry	entry	NN
cord-288669-46tkedw7	89	13	is	be	VBZ
cord-288669-46tkedw7	89	14	interrupted	interrupt	VBN
cord-288669-46tkedw7	89	15	in	in	IN
cord-288669-46tkedw7	89	16	the	the	DT
cord-288669-46tkedw7	89	17	absence	absence	NN
cord-288669-46tkedw7	89	18	of	of	IN
cord-288669-46tkedw7	89	19	E	e	NN
cord-288669-46tkedw7	89	20	protein	protein	NN
cord-288669-46tkedw7	89	21	expression	expression	NN
cord-288669-46tkedw7	89	22	.	.	.
cord-288669-46tkedw7	90	1	We	-PRON-	PRP
cord-288669-46tkedw7	90	2	next	next	RB
cord-288669-46tkedw7	90	3	examined	examine	VBD
cord-288669-46tkedw7	90	4	whether	whether	IN
cord-288669-46tkedw7	90	5	the	the	DT
cord-288669-46tkedw7	90	6	infectivity	infectivity	NN
cord-288669-46tkedw7	90	7	of	of	IN
cord-288669-46tkedw7	90	8	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	90	9	could	could	MD
cord-288669-46tkedw7	90	10	be	be	VB
cord-288669-46tkedw7	90	11	restored	restore	VBN
cord-288669-46tkedw7	90	12	by	by	IN
cord-288669-46tkedw7	90	13	provision	provision	NN
cord-288669-46tkedw7	90	14	of	of	IN
cord-288669-46tkedw7	90	15	the	the	DT
cord-288669-46tkedw7	90	16	E	e	NN
cord-288669-46tkedw7	90	17	protein	protein	NN
cord-288669-46tkedw7	90	18	in	in	IN
cord-288669-46tkedw7	90	19	trans	trans	NNP
cord-288669-46tkedw7	90	20	.	.	.
cord-288669-46tkedw7	91	1	The	the	DT
cord-288669-46tkedw7	91	2	E	e	NN
cord-288669-46tkedw7	91	3	gene	gene	NN
cord-288669-46tkedw7	91	4	was	be	VBD
cord-288669-46tkedw7	91	5	expressed	express	VBN
cord-288669-46tkedw7	91	6	in	in	IN
cord-288669-46tkedw7	91	7	BHK	BHK	NNP
cord-288669-46tkedw7	91	8	-	-	HYPH
cord-288669-46tkedw7	91	9	T7	T7	NNP
cord-288669-46tkedw7	91	10	cells	cell	NNS
cord-288669-46tkedw7	91	11	and	and	CC
cord-288669-46tkedw7	91	12	the	the	DT
cord-288669-46tkedw7	91	13	expression	expression	NN
cord-288669-46tkedw7	91	14	was	be	VBD
cord-288669-46tkedw7	91	15	confirmed	confirm	VBN
cord-288669-46tkedw7	91	16	by	by	IN
cord-288669-46tkedw7	91	17	immunofluorescence	immunofluorescence	NN
cord-288669-46tkedw7	91	18	staining	staining	NN
cord-288669-46tkedw7	91	19	with	with	IN
cord-288669-46tkedw7	91	20	E	e	NN
cord-288669-46tkedw7	91	21	-	-	JJ
cord-288669-46tkedw7	91	22	specific	specific	JJ
cord-288669-46tkedw7	91	23	antiserum	antiserum	NN
cord-288669-46tkedw7	91	24	(	(	-LRB-
cord-288669-46tkedw7	91	25	Fig	Fig	NNP
cord-288669-46tkedw7	91	26	.	.	.
cord-288669-46tkedw7	91	27	2A	2a	CD
cord-288669-46tkedw7	91	28	)	)	-RRB-
cord-288669-46tkedw7	91	29	.	.	.
cord-288669-46tkedw7	92	1	BHK	BHK	NNP
cord-288669-46tkedw7	92	2	-	-	HYPH
cord-288669-46tkedw7	92	3	T7	t7	NN
cord-288669-46tkedw7	92	4	cells	cell	NNS
cord-288669-46tkedw7	92	5	were	be	VBD
cord-288669-46tkedw7	92	6	also	also	RB
cord-288669-46tkedw7	92	7	suitable	suitable	JJ
cord-288669-46tkedw7	92	8	for	for	IN
cord-288669-46tkedw7	92	9	transfection	transfection	NN
cord-288669-46tkedw7	92	10	of	of	IN
cord-288669-46tkedw7	92	11	the	the	DT
cord-288669-46tkedw7	92	12	full	full	JJ
cord-288669-46tkedw7	92	13	-	-	HYPH
cord-288669-46tkedw7	92	14	length	length	NN
cord-288669-46tkedw7	92	15	cDNA	cdna	NN
cord-288669-46tkedw7	92	16	clone	clone	NN
cord-288669-46tkedw7	92	17	as	as	IN
cord-288669-46tkedw7	92	18	N	n	CD
cord-288669-46tkedw7	92	19	-	-	HYPH
cord-288669-46tkedw7	92	20	specific	specific	JJ
cord-288669-46tkedw7	92	21	staining	staining	NN
cord-288669-46tkedw7	92	22	was	be	VBD
cord-288669-46tkedw7	92	23	detected	detect	VBN
cord-288669-46tkedw7	92	24	in	in	IN
cord-288669-46tkedw7	92	25	many	many	JJ
cord-288669-46tkedw7	92	26	cells	cell	NNS
cord-288669-46tkedw7	92	27	(	(	-LRB-
cord-288669-46tkedw7	92	28	Figs	fig	NNS
cord-288669-46tkedw7	92	29	.	.	.
cord-288669-46tkedw7	92	30	2B	2b	CD
cord-288669-46tkedw7	92	31	and	and	CC
cord-288669-46tkedw7	92	32	E	E	NNP
cord-288669-46tkedw7	92	33	)	)	-RRB-
cord-288669-46tkedw7	92	34	.	.	.
cord-288669-46tkedw7	93	1	To	to	TO
cord-288669-46tkedw7	93	2	determine	determine	VB
cord-288669-46tkedw7	93	3	whether	whether	IN
cord-288669-46tkedw7	93	4	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	93	5	in	in	IN
cord-288669-46tkedw7	93	6	the	the	DT
cord-288669-46tkedw7	93	7	E	e	NN
cord-288669-46tkedw7	93	8	gene	gene	NN
cord-288669-46tkedw7	93	9	-	-	HYPH
cord-288669-46tkedw7	93	10	transfected	transfecte	VBN
cord-288669-46tkedw7	93	11	cells	cell	NNS
cord-288669-46tkedw7	93	12	leads	lead	VBZ
cord-288669-46tkedw7	93	13	to	to	IN
cord-288669-46tkedw7	93	14	the	the	DT
cord-288669-46tkedw7	93	15	production	production	NN
cord-288669-46tkedw7	93	16	of	of	IN
cord-288669-46tkedw7	93	17	infectious	infectious	JJ
cord-288669-46tkedw7	93	18	virus	virus	NN
cord-288669-46tkedw7	93	19	particles	particle	NNS
cord-288669-46tkedw7	93	20	,	,	,
cord-288669-46tkedw7	93	21	BHK	BHK	NNP
cord-288669-46tkedw7	93	22	-	-	HYPH
cord-288669-46tkedw7	93	23	T7	T7	NNP
cord-288669-46tkedw7	93	24	cells	cell	NNS
cord-288669-46tkedw7	93	25	were	be	VBD
cord-288669-46tkedw7	93	26	co	co	JJ
cord-288669-46tkedw7	93	27	-	-	VBN
cord-288669-46tkedw7	93	28	transfected	transfecte	VBN
cord-288669-46tkedw7	93	29	with	with	IN
cord-288669-46tkedw7	93	30	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	93	31	and	and	CC
cord-288669-46tkedw7	93	32	E	e	NN
cord-288669-46tkedw7	93	33	gene	gene	NN
cord-288669-46tkedw7	93	34	.	.	.
cord-288669-46tkedw7	94	1	The	the	DT
cord-288669-46tkedw7	94	2	culture	culture	NN
cord-288669-46tkedw7	94	3	supernatant	supernatant	NN
cord-288669-46tkedw7	94	4	was	be	VBD
cord-288669-46tkedw7	94	5	harvested	harvest	VBN
cord-288669-46tkedw7	94	6	at	at	IN
cord-288669-46tkedw7	94	7	48	48	CD
cord-288669-46tkedw7	94	8	h	h	NN
cord-288669-46tkedw7	94	9	post	post	NN
cord-288669-46tkedw7	94	10	-	-	NN
cord-288669-46tkedw7	94	11	transfection	transfection	NN
cord-288669-46tkedw7	94	12	from	from	IN
cord-288669-46tkedw7	94	13	BHK	BHK	NNP
cord-288669-46tkedw7	94	14	-	-	HYPH
cord-288669-46tkedw7	94	15	T7	t7	NN
cord-288669-46tkedw7	94	16	cells	cell	NNS
cord-288669-46tkedw7	94	17	and	and	CC
cord-288669-46tkedw7	94	18	transferred	transfer	VBN
cord-288669-46tkedw7	94	19	to	to	IN
cord-288669-46tkedw7	94	20	Marc-145	marc-145	DT
cord-288669-46tkedw7	94	21	cells	cell	NNS
cord-288669-46tkedw7	94	22	.	.	.
cord-288669-46tkedw7	95	1	At	at	IN
cord-288669-46tkedw7	95	2	24	24	CD
cord-288669-46tkedw7	95	3	h	h	NN
cord-288669-46tkedw7	95	4	postinoculation	postinoculation	NN
cord-288669-46tkedw7	95	5	,	,	,
cord-288669-46tkedw7	95	6	Marc-145	marc-145	NN
cord-288669-46tkedw7	95	7	cells	cell	NNS
cord-288669-46tkedw7	95	8	were	be	VBD
cord-288669-46tkedw7	95	9	fixed	fix	VBN
cord-288669-46tkedw7	95	10	,	,	,
cord-288669-46tkedw7	95	11	and	and	CC
cord-288669-46tkedw7	95	12	virus	virus	NN
cord-288669-46tkedw7	95	13	infection	infection	NN
cord-288669-46tkedw7	95	14	was	be	VBD
cord-288669-46tkedw7	95	15	examined	examine	VBN
cord-288669-46tkedw7	95	16	by	by	IN
cord-288669-46tkedw7	95	17	immunofluorescent	immunofluorescent	NN
cord-288669-46tkedw7	95	18	staining	stain	VBG
cord-288669-46tkedw7	95	19	with	with	IN
cord-288669-46tkedw7	95	20	N	n	CD
cord-288669-46tkedw7	95	21	-	-	HYPH
cord-288669-46tkedw7	95	22	specific	specific	JJ
cord-288669-46tkedw7	95	23	MAb	MAb	NNS
cord-288669-46tkedw7	95	24	.	.	.
cord-288669-46tkedw7	96	1	Marc-145	marc-145	NN
cord-288669-46tkedw7	96	2	cells	cell	NNS
cord-288669-46tkedw7	96	3	inoculated	inoculate	VBN
cord-288669-46tkedw7	96	4	with	with	IN
cord-288669-46tkedw7	96	5	a	a	DT
cord-288669-46tkedw7	96	6	culture	culture	NN
cord-288669-46tkedw7	96	7	fluid	fluid	NN
cord-288669-46tkedw7	96	8	from	from	IN
cord-288669-46tkedw7	96	9	cells	cell	NNS
cord-288669-46tkedw7	96	10	cotransfected	cotransfecte	VBN
cord-288669-46tkedw7	96	11	with	with	IN
cord-288669-46tkedw7	96	12	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	96	13	and	and	CC
cord-288669-46tkedw7	96	14	E	e	NN
cord-288669-46tkedw7	96	15	gene	gene	NN
cord-288669-46tkedw7	96	16	showed	show	VBD
cord-288669-46tkedw7	96	17	bright	bright	JJ
cord-288669-46tkedw7	96	18	N	n	CD
cord-288669-46tkedw7	96	19	-	-	HYPH
cord-288669-46tkedw7	96	20	specific	specific	JJ
cord-288669-46tkedw7	96	21	fluorescent	fluorescent	JJ
cord-288669-46tkedw7	96	22	signal	signal	NN
cord-288669-46tkedw7	96	23	,	,	,
cord-288669-46tkedw7	96	24	indicating	indicate	VBG
cord-288669-46tkedw7	96	25	that	that	IN
cord-288669-46tkedw7	96	26	the	the	DT
cord-288669-46tkedw7	96	27	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	96	28	replication	replication	NN
cord-288669-46tkedw7	96	29	may	may	MD
cord-288669-46tkedw7	96	30	be	be	VB
cord-288669-46tkedw7	96	31	rescued	rescue	VBN
cord-288669-46tkedw7	96	32	by	by	IN
cord-288669-46tkedw7	96	33	trans	trans	NN
cord-288669-46tkedw7	96	34	-	-	NN
cord-288669-46tkedw7	96	35	complementation	complementation	NN
cord-288669-46tkedw7	96	36	of	of	IN
cord-288669-46tkedw7	96	37	the	the	DT
cord-288669-46tkedw7	96	38	E	e	NN
cord-288669-46tkedw7	96	39	protein	protein	NN
cord-288669-46tkedw7	96	40	(	(	-LRB-
cord-288669-46tkedw7	96	41	Fig	Fig	NNP
cord-288669-46tkedw7	96	42	.	.	.
cord-288669-46tkedw7	96	43	 	 	_SP
cord-288669-46tkedw7	96	44	2C	2C	NNP
cord-288669-46tkedw7	96	45	)	)	-RRB-
cord-288669-46tkedw7	96	46	.	.	.
cord-288669-46tkedw7	97	1	In	in	IN
cord-288669-46tkedw7	97	2	contrast	contrast	NN
cord-288669-46tkedw7	97	3	,	,	,
cord-288669-46tkedw7	97	4	in	in	IN
cord-288669-46tkedw7	97	5	cells	cell	NNS
cord-288669-46tkedw7	97	6	inoculated	inoculate	VBN
cord-288669-46tkedw7	97	7	with	with	IN
cord-288669-46tkedw7	97	8	the	the	DT
cord-288669-46tkedw7	97	9	supernatant	supernatant	NN
cord-288669-46tkedw7	97	10	from	from	IN
cord-288669-46tkedw7	97	11	co	co	NN
cord-288669-46tkedw7	97	12	-	-	NN
cord-288669-46tkedw7	97	13	transfection	transfection	NN
cord-288669-46tkedw7	97	14	of	of	IN
cord-288669-46tkedw7	97	15	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	97	16	and	and	CC
cord-288669-46tkedw7	97	17	N	n	CD
cord-288669-46tkedw7	97	18	gene	gene	NN
cord-288669-46tkedw7	97	19	(	(	-LRB-
cord-288669-46tkedw7	97	20	Fig	Fig	NNP
cord-288669-46tkedw7	97	21	.	.	.
cord-288669-46tkedw7	97	22	2F	2F	NNP
cord-288669-46tkedw7	97	23	)	)	-RRB-
cord-288669-46tkedw7	97	24	,	,	,
cord-288669-46tkedw7	97	25	or	or	CC
cord-288669-46tkedw7	97	26	of	of	IN
cord-288669-46tkedw7	97	27	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	97	28	and	and	CC
cord-288669-46tkedw7	97	29	an	an	DT
cord-288669-46tkedw7	97	30	empty	empty	JJ
cord-288669-46tkedw7	97	31	plasmid	plasmid	NN
cord-288669-46tkedw7	97	32	(	(	-LRB-
cord-288669-46tkedw7	97	33	data	datum	NNS
cord-288669-46tkedw7	97	34	not	not	RB
cord-288669-46tkedw7	97	35	shown	show	VBN
cord-288669-46tkedw7	97	36	)	)	-RRB-
cord-288669-46tkedw7	97	37	,	,	,
cord-288669-46tkedw7	97	38	no	no	DT
cord-288669-46tkedw7	97	39	staining	staining	NN
cord-288669-46tkedw7	97	40	was	be	VBD
cord-288669-46tkedw7	97	41	observed	observe	VBN
cord-288669-46tkedw7	97	42	.	.	.
cord-288669-46tkedw7	98	1	The	the	DT
cord-288669-46tkedw7	98	2	staining	staining	NN
cord-288669-46tkedw7	98	3	of	of	IN
cord-288669-46tkedw7	98	4	Marc-145	marc-145	CD
cord-288669-46tkedw7	98	5	cells	cell	NNS
cord-288669-46tkedw7	98	6	inoculated	inoculate	VBN
cord-288669-46tkedw7	98	7	with	with	IN
cord-288669-46tkedw7	98	8	the	the	DT
cord-288669-46tkedw7	98	9	supernatant	supernatant	NN
cord-288669-46tkedw7	98	10	from	from	IN
cord-288669-46tkedw7	98	11	BHK	BHK	NNP
cord-288669-46tkedw7	98	12	cells	cell	NNS
cord-288669-46tkedw7	98	13	co	co	VBP
cord-288669-46tkedw7	98	14	-	-	VBN
cord-288669-46tkedw7	98	15	transfected	transfecte	VBN
cord-288669-46tkedw7	98	16	with	with	IN
cord-288669-46tkedw7	98	17	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	98	18	and	and	CC
cord-288669-46tkedw7	98	19	N	n	CD
cord-288669-46tkedw7	98	20	gene	gene	NN
cord-288669-46tkedw7	98	21	was	be	VBD
cord-288669-46tkedw7	98	22	however	however	RB
cord-288669-46tkedw7	98	23	limited	limit	VBN
cord-288669-46tkedw7	98	24	to	to	IN
cord-288669-46tkedw7	98	25	single	single	JJ
cord-288669-46tkedw7	98	26	cells	cell	NNS
cord-288669-46tkedw7	98	27	and	and	CC
cord-288669-46tkedw7	98	28	no	no	DT
cord-288669-46tkedw7	98	29	infectivity	infectivity	NN
cord-288669-46tkedw7	98	30	was	be	VBD
cord-288669-46tkedw7	98	31	produced	produce	VBN
cord-288669-46tkedw7	98	32	for	for	IN
cord-288669-46tkedw7	98	33	up	up	IN
cord-288669-46tkedw7	98	34	to	to	TO
cord-288669-46tkedw7	98	35	7	7	CD
cord-288669-46tkedw7	98	36	days	day	NNS
cord-288669-46tkedw7	98	37	post	post	NN
cord-288669-46tkedw7	98	38	-	-	NN
cord-288669-46tkedw7	98	39	inoculation	inoculation	JJ
cord-288669-46tkedw7	98	40	(	(	-LRB-
cord-288669-46tkedw7	98	41	Fig	Fig	NNP
cord-288669-46tkedw7	98	42	.	.	.
cord-288669-46tkedw7	98	43	2C	2C	NNP
cord-288669-46tkedw7	98	44	)	)	-RRB-
cord-288669-46tkedw7	98	45	,	,	,
cord-288669-46tkedw7	98	46	indicating	indicate	VBG
cord-288669-46tkedw7	98	47	abortive	abortive	JJ
cord-288669-46tkedw7	98	48	infection	infection	NN
cord-288669-46tkedw7	98	49	of	of	IN
cord-288669-46tkedw7	98	50	the	the	DT
cord-288669-46tkedw7	98	51	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	98	52	mutant	mutant	JJ
cord-288669-46tkedw7	98	53	virus	virus	NN
cord-288669-46tkedw7	98	54	in	in	IN
cord-288669-46tkedw7	98	55	non	non	JJ
cord-288669-46tkedw7	98	56	-	-	JJ
cord-288669-46tkedw7	98	57	complementing	complement	VBG
cord-288669-46tkedw7	98	58	Marc-145	marc-145	CD
cord-288669-46tkedw7	98	59	cells	cell	NNS
cord-288669-46tkedw7	98	60	.	.	.
cord-288669-46tkedw7	99	1	Attempts	attempt	NNS
cord-288669-46tkedw7	99	2	to	to	TO
cord-288669-46tkedw7	99	3	pass	pass	VB
cord-288669-46tkedw7	99	4	the	the	DT
cord-288669-46tkedw7	99	5	passage-1	passage-1	NNP
cord-288669-46tkedw7	99	6	culture	culture	NN
cord-288669-46tkedw7	99	7	supernatant	supernatant	JJ
cord-288669-46tkedw7	99	8	to	to	IN
cord-288669-46tkedw7	100	1	Marc-145	Marc-145	NNP
cord-288669-46tkedw7	100	2	resulted	result	VBD
cord-288669-46tkedw7	100	3	in	in	IN
cord-288669-46tkedw7	100	4	no	no	DT
cord-288669-46tkedw7	100	5	virus	virus	NN
cord-288669-46tkedw7	100	6	replication	replication	NN
cord-288669-46tkedw7	100	7	,	,	,
cord-288669-46tkedw7	100	8	confirming	confirm	VBG
cord-288669-46tkedw7	100	9	that	that	IN
cord-288669-46tkedw7	100	10	the	the	DT
cord-288669-46tkedw7	100	11	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	100	12	virus	virus	NN
cord-288669-46tkedw7	100	13	particles	particle	NNS
cord-288669-46tkedw7	100	14	generated	generate	VBN
cord-288669-46tkedw7	100	15	by	by	IN
cord-288669-46tkedw7	100	16	the	the	DT
cord-288669-46tkedw7	100	17	provision	provision	NN
cord-288669-46tkedw7	100	18	of	of	IN
cord-288669-46tkedw7	100	19	E	E	NNP
cord-288669-46tkedw7	100	20	in	in	IN
cord-288669-46tkedw7	100	21	trans	trans	NNP
cord-288669-46tkedw7	100	22	was	be	VBD
cord-288669-46tkedw7	100	23	only	only	RB
cord-288669-46tkedw7	100	24	capable	capable	JJ
cord-288669-46tkedw7	100	25	of	of	IN
cord-288669-46tkedw7	100	26	a	a	DT
cord-288669-46tkedw7	100	27	single	single	JJ
cord-288669-46tkedw7	100	28	round	round	NN
cord-288669-46tkedw7	100	29	of	of	IN
cord-288669-46tkedw7	100	30	replication	replication	NN
cord-288669-46tkedw7	100	31	.	.	.
cord-288669-46tkedw7	101	1	These	these	DT
cord-288669-46tkedw7	101	2	data	datum	NNS
cord-288669-46tkedw7	101	3	demonstrated	demonstrate	VBD
cord-288669-46tkedw7	101	4	that	that	IN
cord-288669-46tkedw7	101	5	the	the	DT
cord-288669-46tkedw7	101	6	inability	inability	NN
cord-288669-46tkedw7	101	7	of	of	IN
cord-288669-46tkedw7	101	8	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	101	9	to	to	TO
cord-288669-46tkedw7	101	10	produce	produce	VB
cord-288669-46tkedw7	101	11	infectious	infectious	JJ
cord-288669-46tkedw7	101	12	virus	virus	NN
cord-288669-46tkedw7	101	13	was	be	VBD
cord-288669-46tkedw7	101	14	due	due	JJ
cord-288669-46tkedw7	101	15	to	to	IN
cord-288669-46tkedw7	101	16	the	the	DT
cord-288669-46tkedw7	101	17	absence	absence	NN
cord-288669-46tkedw7	101	18	of	of	IN
cord-288669-46tkedw7	101	19	E	e	NN
cord-288669-46tkedw7	101	20	protein	protein	NN
cord-288669-46tkedw7	101	21	in	in	IN
cord-288669-46tkedw7	101	22	the	the	DT
cord-288669-46tkedw7	101	23	virions	virion	NNS
cord-288669-46tkedw7	101	24	.	.	.
cord-288669-46tkedw7	102	1	Because	because	IN
cord-288669-46tkedw7	102	2	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	102	3	did	do	VBD
cord-288669-46tkedw7	102	4	not	not	RB
cord-288669-46tkedw7	102	5	induce	induce	VB
cord-288669-46tkedw7	102	6	infectivity	infectivity	NN
cord-288669-46tkedw7	102	7	from	from	IN
cord-288669-46tkedw7	102	8	transfection	transfection	NN
cord-288669-46tkedw7	102	9	,	,	,
cord-288669-46tkedw7	102	10	it	-PRON-	PRP
cord-288669-46tkedw7	102	11	was	be	VBD
cord-288669-46tkedw7	102	12	of	of	IN
cord-288669-46tkedw7	102	13	interest	interest	NN
cord-288669-46tkedw7	102	14	to	to	TO
cord-288669-46tkedw7	102	15	examine	examine	VB
cord-288669-46tkedw7	102	16	whether	whether	IN
cord-288669-46tkedw7	102	17	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	102	18	particles	particle	NNS
cord-288669-46tkedw7	102	19	were	be	VBD
cord-288669-46tkedw7	102	20	not	not	RB
cord-288669-46tkedw7	102	21	produced	produce	VBN
cord-288669-46tkedw7	102	22	in	in	IN
cord-288669-46tkedw7	102	23	the	the	DT
cord-288669-46tkedw7	102	24	absence	absence	NN
cord-288669-46tkedw7	102	25	of	of	IN
cord-288669-46tkedw7	102	26	E	e	NN
cord-288669-46tkedw7	102	27	protein	protein	NN
cord-288669-46tkedw7	102	28	.	.	.
cord-288669-46tkedw7	103	1	Due	due	IN
cord-288669-46tkedw7	103	2	to	to	IN
cord-288669-46tkedw7	103	3	a	a	DT
cord-288669-46tkedw7	103	4	low	low	JJ
cord-288669-46tkedw7	103	5	transfection	transfection	NN
cord-288669-46tkedw7	103	6	efficiency	efficiency	NN
cord-288669-46tkedw7	103	7	in	in	IN
cord-288669-46tkedw7	103	8	Marc-145	marc-145	DT
cord-288669-46tkedw7	103	9	cells	cell	NNS
cord-288669-46tkedw7	103	10	,	,	,
cord-288669-46tkedw7	103	11	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	103	12	cells	cell	NNS
cord-288669-46tkedw7	103	13	were	be	VBD
cord-288669-46tkedw7	103	14	used	use	VBN
cord-288669-46tkedw7	103	15	to	to	TO
cord-288669-46tkedw7	103	16	achieve	achieve	VB
cord-288669-46tkedw7	103	17	higher	high	JJR
cord-288669-46tkedw7	103	18	transfection	transfection	NN
cord-288669-46tkedw7	103	19	efficiency	efficiency	NN
cord-288669-46tkedw7	103	20	.	.	.
cord-288669-46tkedw7	104	1	When	when	WRB
cord-288669-46tkedw7	104	2	stained	stain	VBN
cord-288669-46tkedw7	104	3	with	with	IN
cord-288669-46tkedw7	104	4	N	n	NN
cord-288669-46tkedw7	104	5	-	-	HYPH
cord-288669-46tkedw7	104	6	specific	specific	JJ
cord-288669-46tkedw7	104	7	MAb	MAb	NNS
cord-288669-46tkedw7	104	8	at	at	IN
cord-288669-46tkedw7	104	9	2	2	CD
cord-288669-46tkedw7	104	10	days	day	NNS
cord-288669-46tkedw7	104	11	post	post	NN
cord-288669-46tkedw7	104	12	-	-	JJ
cord-288669-46tkedw7	104	13	transfection	transfection	JJ
cord-288669-46tkedw7	104	14	,	,	,
cord-288669-46tkedw7	104	15	numerous	numerous	JJ
cord-288669-46tkedw7	104	16	cells	cell	NNS
cord-288669-46tkedw7	104	17	showed	show	VBD
cord-288669-46tkedw7	104	18	N	n	CD
cord-288669-46tkedw7	104	19	-	-	HYPH
cord-288669-46tkedw7	104	20	specific	specific	JJ
cord-288669-46tkedw7	104	21	fluorescence	fluorescence	NN
cord-288669-46tkedw7	104	22	(	(	-LRB-
cord-288669-46tkedw7	104	23	data	datum	NNS
cord-288669-46tkedw7	104	24	not	not	RB
cord-288669-46tkedw7	104	25	shown	show	VBN
cord-288669-46tkedw7	104	26	)	)	-RRB-
cord-288669-46tkedw7	104	27	,	,	,
cord-288669-46tkedw7	104	28	indicating	indicate	VBG
cord-288669-46tkedw7	104	29	high	high	JJ
cord-288669-46tkedw7	104	30	levels	level	NNS
cord-288669-46tkedw7	104	31	of	of	IN
cord-288669-46tkedw7	104	32	transfection	transfection	NN
cord-288669-46tkedw7	104	33	in	in	IN
cord-288669-46tkedw7	104	34	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	104	35	cells	cell	NNS
cord-288669-46tkedw7	104	36	,	,	,
cord-288669-46tkedw7	104	37	which	which	WDT
cord-288669-46tkedw7	104	38	may	may	MD
cord-288669-46tkedw7	104	39	be	be	VB
cord-288669-46tkedw7	104	40	sufficient	sufficient	JJ
cord-288669-46tkedw7	104	41	for	for	IN
cord-288669-46tkedw7	104	42	the	the	DT
cord-288669-46tkedw7	104	43	study	study	NN
cord-288669-46tkedw7	104	44	of	of	IN
cord-288669-46tkedw7	104	45	particle	particle	NN
cord-288669-46tkedw7	104	46	formation	formation	NN
cord-288669-46tkedw7	104	47	.	.	.
cord-288669-46tkedw7	105	1	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	106	1	cells	cell	NNS
cord-288669-46tkedw7	106	2	were	be	VBD
cord-288669-46tkedw7	106	3	transfected	transfecte	VBN
cord-288669-46tkedw7	106	4	with	with	IN
cord-288669-46tkedw7	106	5	P129-WT	p129-wt	CD
cord-288669-46tkedw7	106	6	or	or	CC
cord-288669-46tkedw7	106	7	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	106	8	,	,	,
cord-288669-46tkedw7	106	9	and	and	CC
cord-288669-46tkedw7	106	10	at	at	IN
cord-288669-46tkedw7	106	11	30	30	CD
cord-288669-46tkedw7	106	12	h	h	NN
cord-288669-46tkedw7	106	13	post	post	NN
cord-288669-46tkedw7	106	14	-	-	NN
cord-288669-46tkedw7	106	15	transfection	transfection	JJ
cord-288669-46tkedw7	106	16	,	,	,
cord-288669-46tkedw7	106	17	the	the	DT
cord-288669-46tkedw7	106	18	transfected	transfecte	VBN
cord-288669-46tkedw7	106	19	cells	cell	NNS
cord-288669-46tkedw7	106	20	were	be	VBD
cord-288669-46tkedw7	106	21	radiolabeled	radiolabele	VBN
cord-288669-46tkedw7	106	22	for	for	IN
cord-288669-46tkedw7	106	23	1	1	CD
cord-288669-46tkedw7	106	24	day	day	NN
cord-288669-46tkedw7	106	25	with	with	IN
cord-288669-46tkedw7	106	26	[	[	-LRB-
cord-288669-46tkedw7	106	27	35	35	CD
cord-288669-46tkedw7	106	28	S]methionine	S]methionine	NNP
cord-288669-46tkedw7	106	29	/	/	SYM
cord-288669-46tkedw7	106	30	cysteine	cysteine	NN
cord-288669-46tkedw7	106	31	.	.	.
cord-288669-46tkedw7	107	1	The	the	DT
cord-288669-46tkedw7	107	2	cells	cell	NNS
cord-288669-46tkedw7	107	3	were	be	VBD
cord-288669-46tkedw7	107	4	harvested	harvest	VBN
cord-288669-46tkedw7	107	5	and	and	CC
cord-288669-46tkedw7	107	6	lysed	lyse	VBN
cord-288669-46tkedw7	107	7	with	with	IN
cord-288669-46tkedw7	107	8	RIPA	RIPA	NNP
cord-288669-46tkedw7	107	9	buffer	buffer	NN
cord-288669-46tkedw7	107	10	.	.	.
cord-288669-46tkedw7	108	1	The	the	DT
cord-288669-46tkedw7	108	2	culture	culture	NN
cord-288669-46tkedw7	108	3	supernatants	supernatant	NNS
cord-288669-46tkedw7	108	4	were	be	VBD
cord-288669-46tkedw7	108	5	collected	collect	VBN
cord-288669-46tkedw7	108	6	separately	separately	RB
cord-288669-46tkedw7	108	7	and	and	CC
cord-288669-46tkedw7	108	8	centrifuged	centrifuge	VBN
cord-288669-46tkedw7	108	9	through	through	IN
cord-288669-46tkedw7	108	10	a	a	DT
cord-288669-46tkedw7	108	11	20	20	CD
cord-288669-46tkedw7	108	12	%	%	NN
cord-288669-46tkedw7	108	13	(	(	-LRB-
cord-288669-46tkedw7	108	14	wt	wt	NN
cord-288669-46tkedw7	108	15	/	/	SYM
cord-288669-46tkedw7	108	16	vol	vol	NN
cord-288669-46tkedw7	108	17	)	)	-RRB-
cord-288669-46tkedw7	109	1	sucrose	sucrose	NN
cord-288669-46tkedw7	109	2	cushion	cushion	NN
cord-288669-46tkedw7	109	3	,	,	,
cord-288669-46tkedw7	109	4	and	and	CC
cord-288669-46tkedw7	109	5	the	the	DT
cord-288669-46tkedw7	109	6	resulting	result	VBG
cord-288669-46tkedw7	109	7	pellets	pellet	NNS
cord-288669-46tkedw7	109	8	were	be	VBD
cord-288669-46tkedw7	109	9	dissolved	dissolve	VBN
cord-288669-46tkedw7	109	10	in	in	IN
cord-288669-46tkedw7	109	11	RIPA	RIPA	NNP
cord-288669-46tkedw7	109	12	buffer	buffer	NN
cord-288669-46tkedw7	109	13	.	.	.
cord-288669-46tkedw7	110	1	The	the	DT
cord-288669-46tkedw7	110	2	lysates	lysate	NNS
cord-288669-46tkedw7	110	3	prepared	prepare	VBD
cord-288669-46tkedw7	110	4	from	from	IN
cord-288669-46tkedw7	110	5	cells	cell	NNS
cord-288669-46tkedw7	110	6	or	or	CC
cord-288669-46tkedw7	110	7	supernatants	supernatant	NNS
cord-288669-46tkedw7	110	8	were	be	VBD
cord-288669-46tkedw7	110	9	immunoprecipitated	immunoprecipitate	VBN
cord-288669-46tkedw7	110	10	with	with	IN
cord-288669-46tkedw7	110	11	a	a	DT
cord-288669-46tkedw7	110	12	mixture	mixture	NN
cord-288669-46tkedw7	110	13	of	of	IN
cord-288669-46tkedw7	110	14	individual	individual	JJ
cord-288669-46tkedw7	110	15	antibody	antibody	NN
cord-288669-46tkedw7	110	16	specific	specific	JJ
cord-288669-46tkedw7	110	17	for	for	IN
cord-288669-46tkedw7	110	18	E	E	NNP
cord-288669-46tkedw7	110	19	,	,	,
cord-288669-46tkedw7	110	20	N	N	NNP
cord-288669-46tkedw7	110	21	,	,	,
cord-288669-46tkedw7	110	22	or	or	CC
cord-288669-46tkedw7	110	23	M	M	NNP
cord-288669-46tkedw7	110	24	and	and	CC
cord-288669-46tkedw7	110	25	resolved	resolve	VBN
cord-288669-46tkedw7	110	26	by	by	IN
cord-288669-46tkedw7	110	27	SDS	SDS	NNP
cord-288669-46tkedw7	110	28	-	-	HYPH
cord-288669-46tkedw7	110	29	PAGE	PAGE	NNP
cord-288669-46tkedw7	110	30	.	.	.
cord-288669-46tkedw7	111	1	As	as	IN
cord-288669-46tkedw7	111	2	shown	show	VBN
cord-288669-46tkedw7	111	3	in	in	IN
cord-288669-46tkedw7	111	4	Fig	Fig	NNP
cord-288669-46tkedw7	111	5	.	.	.
cord-288669-46tkedw7	112	1	3A	3A	NNP
cord-288669-46tkedw7	112	2	,	,	,
cord-288669-46tkedw7	112	3	intracellular	intracellular	JJ
cord-288669-46tkedw7	112	4	major	major	JJ
cord-288669-46tkedw7	112	5	viral	viral	JJ
cord-288669-46tkedw7	112	6	-	-	HYPH
cord-288669-46tkedw7	112	7	specific	specific	JJ
cord-288669-46tkedw7	112	8	proteins	protein	NNS
cord-288669-46tkedw7	112	9	were	be	VBD
cord-288669-46tkedw7	112	10	detectable	detectable	JJ
cord-288669-46tkedw7	112	11	in	in	IN
cord-288669-46tkedw7	112	12	all	all	DT
cord-288669-46tkedw7	112	13	lysates	lysate	NNS
cord-288669-46tkedw7	112	14	(	(	-LRB-
cord-288669-46tkedw7	112	15	lower	low	JJR
cord-288669-46tkedw7	112	16	panel	panel	NN
cord-288669-46tkedw7	112	17	)	)	-RRB-
cord-288669-46tkedw7	112	18	,	,	,
cord-288669-46tkedw7	112	19	confirming	confirm	VBG
cord-288669-46tkedw7	112	20	the	the	DT
cord-288669-46tkedw7	112	21	ability	ability	NN
cord-288669-46tkedw7	112	22	of	of	IN
cord-288669-46tkedw7	112	23	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	112	24	for	for	IN
cord-288669-46tkedw7	112	25	transcription	transcription	NN
cord-288669-46tkedw7	112	26	.	.	.
cord-288669-46tkedw7	113	1	Similarly	similarly	RB
cord-288669-46tkedw7	113	2	,	,	,
cord-288669-46tkedw7	113	3	in	in	IN
cord-288669-46tkedw7	113	4	the	the	DT
cord-288669-46tkedw7	113	5	culture	culture	NN
cord-288669-46tkedw7	113	6	supernatants	supernatant	NNS
cord-288669-46tkedw7	113	7	,	,	,
cord-288669-46tkedw7	113	8	the	the	DT
cord-288669-46tkedw7	113	9	three	three	CD
cord-288669-46tkedw7	113	10	major	major	JJ
cord-288669-46tkedw7	113	11	virion	virion	NN
cord-288669-46tkedw7	113	12	proteins	protein	NNS
cord-288669-46tkedw7	114	1	GP5	GP5	NNP
cord-288669-46tkedw7	114	2	,	,	,
cord-288669-46tkedw7	114	3	M	M	NNP
cord-288669-46tkedw7	114	4	and	and	CC
cord-288669-46tkedw7	114	5	N	N	NNP
cord-288669-46tkedw7	114	6	were	be	VBD
cord-288669-46tkedw7	114	7	clearly	clearly	RB
cord-288669-46tkedw7	114	8	identified	identify	VBN
cord-288669-46tkedw7	114	9	(	(	-LRB-
cord-288669-46tkedw7	114	10	upper	upper	JJ
cord-288669-46tkedw7	114	11	panel	panel	NN
cord-288669-46tkedw7	114	12	)	)	-RRB-
cord-288669-46tkedw7	114	13	,	,	,
cord-288669-46tkedw7	114	14	showing	show	VBG
cord-288669-46tkedw7	114	15	that	that	IN
cord-288669-46tkedw7	114	16	,	,	,
cord-288669-46tkedw7	114	17	without	without	IN
cord-288669-46tkedw7	114	18	E	e	NN
cord-288669-46tkedw7	114	19	protein	protein	NN
cord-288669-46tkedw7	114	20	,	,	,
cord-288669-46tkedw7	114	21	virus	virus	NN
cord-288669-46tkedw7	114	22	particles	particle	NNS
cord-288669-46tkedw7	114	23	can	can	MD
cord-288669-46tkedw7	114	24	be	be	VB
cord-288669-46tkedw7	114	25	produced	produce	VBN
cord-288669-46tkedw7	114	26	and	and	CC
cord-288669-46tkedw7	114	27	released	release	VBN
cord-288669-46tkedw7	114	28	.	.	.
cord-288669-46tkedw7	115	1	It	-PRON-	PRP
cord-288669-46tkedw7	115	2	was	be	VBD
cord-288669-46tkedw7	115	3	not	not	RB
cord-288669-46tkedw7	115	4	possible	possible	JJ
cord-288669-46tkedw7	115	5	to	to	TO
cord-288669-46tkedw7	115	6	detect	detect	VB
cord-288669-46tkedw7	115	7	the	the	DT
cord-288669-46tkedw7	115	8	E	e	NN
cord-288669-46tkedw7	115	9	protein	protein	NN
cord-288669-46tkedw7	115	10	in	in	IN
cord-288669-46tkedw7	115	11	the	the	DT
cord-288669-46tkedw7	115	12	cell	cell	NN
cord-288669-46tkedw7	115	13	lysates	lysate	NNS
cord-288669-46tkedw7	115	14	or	or	CC
cord-288669-46tkedw7	115	15	supernatants	supernatant	NNS
cord-288669-46tkedw7	115	16	from	from	IN
cord-288669-46tkedw7	115	17	P129-WT	p129-wt	CD
cord-288669-46tkedw7	115	18	-	-	HYPH
cord-288669-46tkedw7	115	19	transfected	transfecte	VBN
cord-288669-46tkedw7	115	20	cells	cell	NNS
cord-288669-46tkedw7	115	21	,	,	,
cord-288669-46tkedw7	115	22	and	and	CC
cord-288669-46tkedw7	115	23	this	this	DT
cord-288669-46tkedw7	115	24	is	be	VBZ
cord-288669-46tkedw7	115	25	probably	probably	RB
cord-288669-46tkedw7	115	26	due	due	JJ
cord-288669-46tkedw7	115	27	to	to	IN
cord-288669-46tkedw7	115	28	the	the	DT
cord-288669-46tkedw7	115	29	low	low	JJ
cord-288669-46tkedw7	115	30	abundance	abundance	NN
cord-288669-46tkedw7	115	31	of	of	IN
cord-288669-46tkedw7	115	32	E	e	NN
cord-288669-46tkedw7	115	33	protein	protein	NN
cord-288669-46tkedw7	115	34	in	in	IN
cord-288669-46tkedw7	115	35	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	115	36	cells	cell	NNS
cord-288669-46tkedw7	115	37	as	as	IN
cord-288669-46tkedw7	115	38	E	E	NNP
cord-288669-46tkedw7	115	39	is	be	VBZ
cord-288669-46tkedw7	115	40	a	a	DT
cord-288669-46tkedw7	115	41	minor	minor	JJ
cord-288669-46tkedw7	115	42	protein	protein	NN
cord-288669-46tkedw7	115	43	(	(	-LRB-
cord-288669-46tkedw7	115	44	Wu	Wu	NNP
cord-288669-46tkedw7	115	45	et	et	NNP
cord-288669-46tkedw7	115	46	al	al	NNP
cord-288669-46tkedw7	115	47	.	.	NNP
cord-288669-46tkedw7	115	48	,	,	,
cord-288669-46tkedw7	115	49	2005	2005	CD
cord-288669-46tkedw7	115	50	)	)	-RRB-
cord-288669-46tkedw7	115	51	.	.	.
cord-288669-46tkedw7	116	1	This	this	DT
cord-288669-46tkedw7	116	2	result	result	NN
cord-288669-46tkedw7	116	3	suggests	suggest	VBZ
cord-288669-46tkedw7	116	4	that	that	IN
cord-288669-46tkedw7	116	5	the	the	DT
cord-288669-46tkedw7	116	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	116	7	E	E	NNP
cord-288669-46tkedw7	116	8	protein	protein	NN
cord-288669-46tkedw7	116	9	is	be	VBZ
cord-288669-46tkedw7	116	10	not	not	RB
cord-288669-46tkedw7	116	11	required	require	VBN
cord-288669-46tkedw7	116	12	for	for	IN
cord-288669-46tkedw7	116	13	particle	particle	NN
cord-288669-46tkedw7	116	14	formation	formation	NN
cord-288669-46tkedw7	116	15	.	.	.
cord-288669-46tkedw7	117	1	Because	because	IN
cord-288669-46tkedw7	117	2	the	the	DT
cord-288669-46tkedw7	117	3	virus	virus	NN
cord-288669-46tkedw7	117	4	particles	particle	NNS
cord-288669-46tkedw7	117	5	formed	form	VBN
cord-288669-46tkedw7	117	6	in	in	IN
cord-288669-46tkedw7	117	7	the	the	DT
cord-288669-46tkedw7	117	8	absence	absence	NN
cord-288669-46tkedw7	117	9	of	of	IN
cord-288669-46tkedw7	117	10	E	e	NN
cord-288669-46tkedw7	117	11	protein	protein	NN
cord-288669-46tkedw7	117	12	were	be	VBD
cord-288669-46tkedw7	117	13	non	non	JJ
cord-288669-46tkedw7	117	14	-	-	JJ
cord-288669-46tkedw7	117	15	infectious	infectious	JJ
cord-288669-46tkedw7	117	16	,	,	,
cord-288669-46tkedw7	117	17	these	these	DT
cord-288669-46tkedw7	117	18	particles	particle	NNS
cord-288669-46tkedw7	117	19	were	be	VBD
cord-288669-46tkedw7	117	20	examined	examine	VBN
cord-288669-46tkedw7	117	21	for	for	IN
cord-288669-46tkedw7	117	22	the	the	DT
cord-288669-46tkedw7	117	23	presence	presence	NN
cord-288669-46tkedw7	117	24	of	of	IN
cord-288669-46tkedw7	117	25	viral	viral	JJ
cord-288669-46tkedw7	117	26	genome	genome	NN
cord-288669-46tkedw7	117	27	.	.	.
cord-288669-46tkedw7	118	1	The	the	DT
cord-288669-46tkedw7	118	2	culture	culture	NN
cord-288669-46tkedw7	118	3	supernatants	supernatant	NNS
cord-288669-46tkedw7	118	4	,	,	,
cord-288669-46tkedw7	118	5	prepared	prepare	VBN
cord-288669-46tkedw7	118	6	from	from	IN
cord-288669-46tkedw7	118	7	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	118	8	cells	cell	NNS
cord-288669-46tkedw7	118	9	transfected	transfecte	VBN
cord-288669-46tkedw7	118	10	with	with	IN
cord-288669-46tkedw7	118	11	P129-WT	p129-wt	CD
cord-288669-46tkedw7	118	12	or	or	CC
cord-288669-46tkedw7	118	13	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	118	14	,	,	,
cord-288669-46tkedw7	118	15	were	be	VBD
cord-288669-46tkedw7	118	16	first	first	RB
cord-288669-46tkedw7	118	17	incubated	incubate	VBN
cord-288669-46tkedw7	118	18	with	with	IN
cord-288669-46tkedw7	118	19	both	both	CC
cord-288669-46tkedw7	118	20	RNase	RNase	NNP
cord-288669-46tkedw7	118	21	A	A	NNP
cord-288669-46tkedw7	118	22	and	and	CC
cord-288669-46tkedw7	118	23	DNase	DNase	NNP
cord-288669-46tkedw7	118	24	I	-PRON-	PRP
cord-288669-46tkedw7	118	25	to	to	TO
cord-288669-46tkedw7	118	26	remove	remove	VB
cord-288669-46tkedw7	118	27	any	any	DT
cord-288669-46tkedw7	118	28	possible	possible	JJ
cord-288669-46tkedw7	118	29	contamination	contamination	NN
cord-288669-46tkedw7	118	30	of	of	IN
cord-288669-46tkedw7	118	31	viral	viral	JJ
cord-288669-46tkedw7	118	32	RNA	RNA	NNP
cord-288669-46tkedw7	118	33	and	and	CC
cord-288669-46tkedw7	118	34	the	the	DT
cord-288669-46tkedw7	118	35	transfected	transfecte	VBN
cord-288669-46tkedw7	118	36	DNA	dna	NN
cord-288669-46tkedw7	118	37	from	from	IN
cord-288669-46tkedw7	118	38	cells	cell	NNS
cord-288669-46tkedw7	118	39	.	.	.
cord-288669-46tkedw7	119	1	The	the	DT
cord-288669-46tkedw7	119	2	digestion	digestion	NN
cord-288669-46tkedw7	119	3	was	be	VBD
cord-288669-46tkedw7	119	4	carried	carry	VBN
cord-288669-46tkedw7	119	5	out	out	RP
cord-288669-46tkedw7	119	6	in	in	IN
cord-288669-46tkedw7	119	7	the	the	DT
cord-288669-46tkedw7	119	8	presence	presence	NN
cord-288669-46tkedw7	119	9	or	or	CC
cord-288669-46tkedw7	119	10	absence	absence	NN
cord-288669-46tkedw7	119	11	of	of	IN
cord-288669-46tkedw7	119	12	detergents	detergent	NNS
cord-288669-46tkedw7	119	13	,	,	,
cord-288669-46tkedw7	119	14	followed	follow	VBN
cord-288669-46tkedw7	119	15	by	by	IN
cord-288669-46tkedw7	119	16	proteinase	proteinase	NN
cord-288669-46tkedw7	119	17	K	k	NN
cord-288669-46tkedw7	119	18	treatment	treatment	NN
cord-288669-46tkedw7	119	19	to	to	TO
cord-288669-46tkedw7	119	20	inactivate	inactivate	VB
cord-288669-46tkedw7	119	21	the	the	DT
cord-288669-46tkedw7	119	22	nucleases	nuclease	NNS
cord-288669-46tkedw7	119	23	.	.	.
cord-288669-46tkedw7	120	1	RNA	RNA	NNP
cord-288669-46tkedw7	120	2	was	be	VBD
cord-288669-46tkedw7	120	3	extracted	extract	VBN
cord-288669-46tkedw7	120	4	from	from	IN
cord-288669-46tkedw7	120	5	samples	sample	NNS
cord-288669-46tkedw7	120	6	and	and	CC
cord-288669-46tkedw7	120	7	treated	treat	VBN
cord-288669-46tkedw7	120	8	again	again	RB
cord-288669-46tkedw7	120	9	with	with	IN
cord-288669-46tkedw7	120	10	DNase	DNase	NNP
cord-288669-46tkedw7	120	11	I	i	NN
cord-288669-46tkedw7	120	12	,	,	,
cord-288669-46tkedw7	120	13	and	and	CC
cord-288669-46tkedw7	120	14	the	the	DT
cord-288669-46tkedw7	120	15	E	e	NN
cord-288669-46tkedw7	120	16	gene	gene	NN
cord-288669-46tkedw7	120	17	region	region	NN
cord-288669-46tkedw7	120	18	of	of	IN
cord-288669-46tkedw7	120	19	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	120	20	was	be	VBD
cord-288669-46tkedw7	120	21	RT	RT	NNP
cord-288669-46tkedw7	120	22	-	-	HYPH
cord-288669-46tkedw7	120	23	PCR	PCR	NNP
cord-288669-46tkedw7	120	24	-	-	HYPH
cord-288669-46tkedw7	120	25	amplified	amplify	VBN
cord-288669-46tkedw7	120	26	and	and	CC
cord-288669-46tkedw7	120	27	sequenced	sequence	VBN
cord-288669-46tkedw7	120	28	.	.	.
cord-288669-46tkedw7	121	1	In	in	IN
cord-288669-46tkedw7	121	2	cell	cell	NN
cord-288669-46tkedw7	121	3	culture	culture	NN
cord-288669-46tkedw7	121	4	media	medium	NNS
cord-288669-46tkedw7	121	5	mixed	mix	VBN
cord-288669-46tkedw7	121	6	with	with	IN
cord-288669-46tkedw7	121	7	the	the	DT
cord-288669-46tkedw7	121	8	full	full	JJ
cord-288669-46tkedw7	121	9	-	-	HYPH
cord-288669-46tkedw7	121	10	length	length	NN
cord-288669-46tkedw7	121	11	cDNA	cdna	NN
cord-288669-46tkedw7	121	12	clone	clone	NN
cord-288669-46tkedw7	121	13	as	as	IN
cord-288669-46tkedw7	121	14	a	a	DT
cord-288669-46tkedw7	121	15	negative	negative	JJ
cord-288669-46tkedw7	121	16	control	control	NN
cord-288669-46tkedw7	121	17	,	,	,
cord-288669-46tkedw7	121	18	no	no	DT
cord-288669-46tkedw7	121	19	PCR	PCR	NNP
cord-288669-46tkedw7	121	20	fragment	fragment	NN
cord-288669-46tkedw7	121	21	was	be	VBD
cord-288669-46tkedw7	121	22	amplified	amplify	VBN
cord-288669-46tkedw7	121	23	in	in	IN
cord-288669-46tkedw7	121	24	the	the	DT
cord-288669-46tkedw7	121	25	presence	presence	NN
cord-288669-46tkedw7	121	26	or	or	CC
cord-288669-46tkedw7	121	27	absence	absence	NN
cord-288669-46tkedw7	121	28	of	of	IN
cord-288669-46tkedw7	121	29	detergents	detergent	NNS
cord-288669-46tkedw7	121	30	(	(	-LRB-
cord-288669-46tkedw7	121	31	Fig	Fig	NNP
cord-288669-46tkedw7	121	32	.	.	.
cord-288669-46tkedw7	121	33	3B	3B	NNP
cord-288669-46tkedw7	121	34	,	,	,
cord-288669-46tkedw7	121	35	lanes	lane	NNS
cord-288669-46tkedw7	121	36	2	2	CD
cord-288669-46tkedw7	121	37	and	and	CC
cord-288669-46tkedw7	121	38	3	3	CD
cord-288669-46tkedw7	121	39	)	)	-RRB-
cord-288669-46tkedw7	121	40	,	,	,
cord-288669-46tkedw7	121	41	indicating	indicate	VBG
cord-288669-46tkedw7	121	42	the	the	DT
cord-288669-46tkedw7	121	43	appropriateness	appropriateness	NN
cord-288669-46tkedw7	121	44	of	of	IN
cord-288669-46tkedw7	121	45	DNase	DNase	NNP
cord-288669-46tkedw7	121	46	I	i	NN
cord-288669-46tkedw7	121	47	treatment	treatment	NN
cord-288669-46tkedw7	121	48	.	.	.
cord-288669-46tkedw7	122	1	Although	although	IN
cord-288669-46tkedw7	122	2	no	no	DT
cord-288669-46tkedw7	122	3	amplification	amplification	NN
cord-288669-46tkedw7	122	4	was	be	VBD
cord-288669-46tkedw7	122	5	identified	identify	VBN
cord-288669-46tkedw7	122	6	for	for	IN
cord-288669-46tkedw7	122	7	controls	control	NNS
cord-288669-46tkedw7	122	8	(	(	-LRB-
cord-288669-46tkedw7	122	9	Fig	Fig	NNP
cord-288669-46tkedw7	122	10	.	.	.
cord-288669-46tkedw7	122	11	3B	3B	NNP
cord-288669-46tkedw7	122	12	,	,	,
cord-288669-46tkedw7	122	13	lanes	lane	NNS
cord-288669-46tkedw7	122	14	5	5	CD
cord-288669-46tkedw7	122	15	and	and	CC
cord-288669-46tkedw7	122	16	7	7	CD
cord-288669-46tkedw7	122	17	)	)	-RRB-
cord-288669-46tkedw7	122	18	,	,	,
cord-288669-46tkedw7	122	19	a	a	DT
cord-288669-46tkedw7	122	20	260-bp	260-bp	CD
cord-288669-46tkedw7	122	21	product	product	NN
cord-288669-46tkedw7	122	22	was	be	VBD
cord-288669-46tkedw7	122	23	specifically	specifically	RB
cord-288669-46tkedw7	122	24	amplified	amplify	VBN
cord-288669-46tkedw7	122	25	for	for	IN
cord-288669-46tkedw7	122	26	both	both	DT
cord-288669-46tkedw7	122	27	samples	sample	NNS
cord-288669-46tkedw7	122	28	of	of	IN
cord-288669-46tkedw7	122	29	P129-WT	p129-wt	CD
cord-288669-46tkedw7	122	30	and	and	CC
cord-288669-46tkedw7	122	31	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	122	32	treated	treat	VBN
cord-288669-46tkedw7	122	33	with	with	IN
cord-288669-46tkedw7	122	34	the	the	DT
cord-288669-46tkedw7	122	35	nucleases	nuclease	NNS
cord-288669-46tkedw7	122	36	but	but	CC
cord-288669-46tkedw7	122	37	in	in	IN
cord-288669-46tkedw7	122	38	the	the	DT
cord-288669-46tkedw7	122	39	absence	absence	NN
cord-288669-46tkedw7	122	40	of	of	IN
cord-288669-46tkedw7	122	41	detergents	detergent	NNS
cord-288669-46tkedw7	122	42	(	(	-LRB-
cord-288669-46tkedw7	122	43	Fig	Fig	NNP
cord-288669-46tkedw7	122	44	.	.	.
cord-288669-46tkedw7	122	45	3B	3B	NNP
cord-288669-46tkedw7	122	46	,	,	,
cord-288669-46tkedw7	122	47	lanes	lane	NNS
cord-288669-46tkedw7	122	48	4	4	CD
cord-288669-46tkedw7	122	49	and	and	CC
cord-288669-46tkedw7	122	50	6	6	CD
cord-288669-46tkedw7	122	51	)	)	-RRB-
cord-288669-46tkedw7	122	52	.	.	.
cord-288669-46tkedw7	123	1	Sequencing	sequencing	NN
cord-288669-46tkedw7	123	2	of	of	IN
cord-288669-46tkedw7	123	3	the	the	DT
cord-288669-46tkedw7	123	4	260-bp	260-bp	CD
cord-288669-46tkedw7	123	5	product	product	NN
cord-288669-46tkedw7	123	6	confirmed	confirm	VBD
cord-288669-46tkedw7	123	7	the	the	DT
cord-288669-46tkedw7	123	8	stable	stable	JJ
cord-288669-46tkedw7	123	9	incorporation	incorporation	NN
cord-288669-46tkedw7	123	10	of	of	IN
cord-288669-46tkedw7	123	11	the	the	DT
cord-288669-46tkedw7	123	12	'	'	``
cord-288669-46tkedw7	123	13	A12062	A12062	NNP
cord-288669-46tkedw7	123	14	G	g	NN
cord-288669-46tkedw7	123	15	'	'	POS
cord-288669-46tkedw7	123	16	mutation	mutation	NN
cord-288669-46tkedw7	123	17	at	at	IN
cord-288669-46tkedw7	123	18	the	the	DT
cord-288669-46tkedw7	123	19	start	start	NN
cord-288669-46tkedw7	123	20	codon	codon	NN
cord-288669-46tkedw7	123	21	of	of	IN
cord-288669-46tkedw7	123	22	the	the	DT
cord-288669-46tkedw7	123	23	E	e	NN
cord-288669-46tkedw7	123	24	gene	gene	NN
cord-288669-46tkedw7	123	25	in	in	IN
cord-288669-46tkedw7	123	26	the	the	DT
cord-288669-46tkedw7	123	27	genome	genome	NN
cord-288669-46tkedw7	123	28	from	from	IN
cord-288669-46tkedw7	123	29	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	123	30	particles	particle	NNS
cord-288669-46tkedw7	123	31	.	.	.
cord-288669-46tkedw7	124	1	These	these	DT
cord-288669-46tkedw7	124	2	studies	study	NNS
cord-288669-46tkedw7	124	3	demonstrate	demonstrate	VBP
cord-288669-46tkedw7	124	4	that	that	IN
cord-288669-46tkedw7	124	5	particles	particle	NNS
cord-288669-46tkedw7	124	6	formed	form	VBN
cord-288669-46tkedw7	124	7	in	in	IN
cord-288669-46tkedw7	124	8	the	the	DT
cord-288669-46tkedw7	124	9	absence	absence	NN
cord-288669-46tkedw7	124	10	of	of	IN
cord-288669-46tkedw7	124	11	E	e	NN
cord-288669-46tkedw7	124	12	protein	protein	NN
cord-288669-46tkedw7	124	13	contained	contain	VBD
cord-288669-46tkedw7	124	14	the	the	DT
cord-288669-46tkedw7	124	15	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	124	16	genomic	genomic	JJ
cord-288669-46tkedw7	124	17	RNA	RNA	NNP
cord-288669-46tkedw7	124	18	,	,	,
cord-288669-46tkedw7	124	19	which	which	WDT
cord-288669-46tkedw7	124	20	in	in	IN
cord-288669-46tkedw7	124	21	turn	turn	NN
cord-288669-46tkedw7	124	22	]	]	-RRB-
cord-288669-46tkedw7	124	23	methionine	methionine	NN
cord-288669-46tkedw7	124	24	/	/	SYM
cord-288669-46tkedw7	124	25	cysteine	cysteine	NN
cord-288669-46tkedw7	124	26	for	for	IN
cord-288669-46tkedw7	124	27	24	24	CD
cord-288669-46tkedw7	124	28	h	h	NN
cord-288669-46tkedw7	124	29	at	at	IN
cord-288669-46tkedw7	124	30	37	37	CD
cord-288669-46tkedw7	124	31	°	°	NN
cord-288669-46tkedw7	124	32	C	c	NN
cord-288669-46tkedw7	124	33	,	,	,
cord-288669-46tkedw7	124	34	and	and	CC
cord-288669-46tkedw7	124	35	the	the	DT
cord-288669-46tkedw7	124	36	supernatants	supernatant	NNS
cord-288669-46tkedw7	124	37	and	and	CC
cord-288669-46tkedw7	124	38	cells	cell	NNS
cord-288669-46tkedw7	124	39	were	be	VBD
cord-288669-46tkedw7	124	40	separately	separately	RB
cord-288669-46tkedw7	124	41	collected	collect	VBN
cord-288669-46tkedw7	124	42	.	.	.
cord-288669-46tkedw7	125	1	Cell	cell	NN
cord-288669-46tkedw7	125	2	lysates	lysate	NNS
cord-288669-46tkedw7	125	3	were	be	VBD
cord-288669-46tkedw7	125	4	prepared	prepare	VBN
cord-288669-46tkedw7	125	5	and	and	CC
cord-288669-46tkedw7	125	6	subjected	subject	VBN
cord-288669-46tkedw7	125	7	to	to	IN
cord-288669-46tkedw7	125	8	immunoprecipitation	immunoprecipitation	NN
cord-288669-46tkedw7	125	9	with	with	IN
cord-288669-46tkedw7	125	10	N	n	CD
cord-288669-46tkedw7	125	11	-	-	HYPH
cord-288669-46tkedw7	125	12	specific	specific	JJ
cord-288669-46tkedw7	125	13	MAb	MAb	NNS
cord-288669-46tkedw7	125	14	(	(	-LRB-
cord-288669-46tkedw7	125	15	lower	low	JJR
cord-288669-46tkedw7	125	16	panel	panel	NN
cord-288669-46tkedw7	125	17	)	)	-RRB-
cord-288669-46tkedw7	125	18	.	.	.
cord-288669-46tkedw7	126	1	The	the	DT
cord-288669-46tkedw7	126	2	pellets	pellet	NNS
cord-288669-46tkedw7	126	3	were	be	VBD
cord-288669-46tkedw7	126	4	prepared	prepare	VBN
cord-288669-46tkedw7	126	5	from	from	IN
cord-288669-46tkedw7	126	6	culture	culture	NN
cord-288669-46tkedw7	126	7	supernatants	supernatant	NNS
cord-288669-46tkedw7	126	8	by	by	IN
cord-288669-46tkedw7	126	9	ultracentrifugation	ultracentrifugation	NN
cord-288669-46tkedw7	126	10	,	,	,
cord-288669-46tkedw7	126	11	lysed	lyse	VBN
cord-288669-46tkedw7	126	12	in	in	IN
cord-288669-46tkedw7	126	13	RIPA	RIPA	NNP
cord-288669-46tkedw7	126	14	buffer	buffer	NN
cord-288669-46tkedw7	126	15	and	and	CC
cord-288669-46tkedw7	126	16	used	use	VBN
cord-288669-46tkedw7	126	17	for	for	IN
cord-288669-46tkedw7	126	18	immunoprecipitation	immunoprecipitation	NN
cord-288669-46tkedw7	126	19	with	with	IN
cord-288669-46tkedw7	126	20	a	a	DT
cord-288669-46tkedw7	126	21	mixture	mixture	NN
cord-288669-46tkedw7	126	22	of	of	IN
cord-288669-46tkedw7	126	23	anti	anti	JJ
cord-288669-46tkedw7	126	24	-	-	JJ
cord-288669-46tkedw7	126	25	M	m	JJ
cord-288669-46tkedw7	126	26	,	,	,
cord-288669-46tkedw7	126	27	anti	anti	JJ
cord-288669-46tkedw7	126	28	-	-	NNP
cord-288669-46tkedw7	126	29	N	n	JJ
cord-288669-46tkedw7	126	30	and	and	CC
cord-288669-46tkedw7	126	31	anti	anti	JJ
cord-288669-46tkedw7	126	32	-	-	JJ
cord-288669-46tkedw7	126	33	E	e	JJ
cord-288669-46tkedw7	126	34	-	-	JJ
cord-288669-46tkedw7	126	35	specific	specific	JJ
cord-288669-46tkedw7	126	36	antibodies	antibody	NNS
cord-288669-46tkedw7	126	37	,	,	,
cord-288669-46tkedw7	126	38	followed	follow	VBN
cord-288669-46tkedw7	126	39	by	by	IN
cord-288669-46tkedw7	126	40	SDS-15	sds-15	CD
cord-288669-46tkedw7	126	41	%	%	NN
cord-288669-46tkedw7	126	42	PAGE	page	NN
cord-288669-46tkedw7	126	43	under	under	IN
cord-288669-46tkedw7	126	44	reducing	reduce	VBG
cord-288669-46tkedw7	126	45	conditions	condition	NNS
cord-288669-46tkedw7	126	46	(	(	-LRB-
cord-288669-46tkedw7	126	47	upper	upper	JJ
cord-288669-46tkedw7	126	48	panel	panel	NN
cord-288669-46tkedw7	126	49	)	)	-RRB-
cord-288669-46tkedw7	126	50	.	.	.
cord-288669-46tkedw7	127	1	Lane	lane	NN
cord-288669-46tkedw7	127	2	1	1	CD
cord-288669-46tkedw7	127	3	,	,	,
cord-288669-46tkedw7	127	4	mock	mock	NN
cord-288669-46tkedw7	127	5	transfected	transfecte	VBN
cord-288669-46tkedw7	127	6	;	;	:
cord-288669-46tkedw7	127	7	lane	lane	NN
cord-288669-46tkedw7	127	8	2	2	CD
cord-288669-46tkedw7	127	9	,	,	,
cord-288669-46tkedw7	127	10	P129-WT	p129-wt	CD
cord-288669-46tkedw7	127	11	transfected	transfecte	VBN
cord-288669-46tkedw7	127	12	;	;	:
cord-288669-46tkedw7	127	13	lane	lane	NN
cord-288669-46tkedw7	127	14	3	3	CD
cord-288669-46tkedw7	127	15	,	,	,
cord-288669-46tkedw7	127	16	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	127	17	transfected	transfecte	VBN
cord-288669-46tkedw7	127	18	.	.	.
cord-288669-46tkedw7	128	1	(	(	-LRB-
cord-288669-46tkedw7	128	2	B	b	NN
cord-288669-46tkedw7	128	3	)	)	-RRB-
cord-288669-46tkedw7	128	4	Incorporation	Incorporation	NNP
cord-288669-46tkedw7	128	5	of	of	IN
cord-288669-46tkedw7	128	6	genomic	genomic	JJ
cord-288669-46tkedw7	128	7	RNA	RNA	NNP
cord-288669-46tkedw7	128	8	in	in	IN
cord-288669-46tkedw7	128	9	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	128	10	virus	virus	NN
cord-288669-46tkedw7	128	11	particles	particle	NNS
cord-288669-46tkedw7	128	12	.	.	.
cord-288669-46tkedw7	129	1	Culture	culture	NN
cord-288669-46tkedw7	129	2	supernatants	supernatant	NNS
cord-288669-46tkedw7	129	3	were	be	VBD
cord-288669-46tkedw7	129	4	pelleted	pellete	VBN
cord-288669-46tkedw7	129	5	by	by	IN
cord-288669-46tkedw7	129	6	ultracentrifugation	ultracentrifugation	NN
cord-288669-46tkedw7	129	7	and	and	CC
cord-288669-46tkedw7	129	8	the	the	DT
cord-288669-46tkedw7	129	9	pellets	pellet	NNS
cord-288669-46tkedw7	129	10	were	be	VBD
cord-288669-46tkedw7	129	11	treated	treat	VBN
cord-288669-46tkedw7	129	12	with	with	IN
cord-288669-46tkedw7	129	13	nucleases	nuclease	NNS
cord-288669-46tkedw7	129	14	in	in	IN
cord-288669-46tkedw7	129	15	the	the	DT
cord-288669-46tkedw7	129	16	presence	presence	NN
cord-288669-46tkedw7	129	17	(	(	-LRB-
cord-288669-46tkedw7	129	18	+	+	NN
cord-288669-46tkedw7	129	19	)	)	-RRB-
cord-288669-46tkedw7	129	20	or	or	CC
cord-288669-46tkedw7	129	21	absence	absence	NN
cord-288669-46tkedw7	129	22	(	(	-LRB-
cord-288669-46tkedw7	129	23	−	−	NN
cord-288669-46tkedw7	129	24	)	)	-RRB-
cord-288669-46tkedw7	129	25	of	of	IN
cord-288669-46tkedw7	129	26	SDS	SDS	NNP
cord-288669-46tkedw7	129	27	and	and	CC
cord-288669-46tkedw7	129	28	Triton	Triton	NNP
cord-288669-46tkedw7	129	29	X-100	X-100	NNS
cord-288669-46tkedw7	129	30	,	,	,
cord-288669-46tkedw7	129	31	followed	follow	VBN
cord-288669-46tkedw7	129	32	by	by	IN
cord-288669-46tkedw7	129	33	proteinase	proteinase	NN
cord-288669-46tkedw7	129	34	K	K	NNP
cord-288669-46tkedw7	129	35	treatment	treatment	NN
cord-288669-46tkedw7	129	36	.	.	.
cord-288669-46tkedw7	130	1	RT	RT	NNP
cord-288669-46tkedw7	130	2	-	-	HYPH
cord-288669-46tkedw7	130	3	PCR	PCR	NNP
cord-288669-46tkedw7	130	4	was	be	VBD
cord-288669-46tkedw7	130	5	conducted	conduct	VBN
cord-288669-46tkedw7	130	6	for	for	IN
cord-288669-46tkedw7	130	7	E	e	NN
cord-288669-46tkedw7	130	8	gene	gene	NN
cord-288669-46tkedw7	130	9	amplification	amplification	NN
cord-288669-46tkedw7	130	10	followed	follow	VBN
cord-288669-46tkedw7	130	11	by	by	IN
cord-288669-46tkedw7	130	12	electrophoresis	electrophoresis	NN
cord-288669-46tkedw7	130	13	in	in	IN
cord-288669-46tkedw7	130	14	1.5	1.5	CD
cord-288669-46tkedw7	130	15	%	%	NN
cord-288669-46tkedw7	130	16	agarose	agarose	NN
cord-288669-46tkedw7	130	17	gel	gel	NN
cord-288669-46tkedw7	130	18	.	.	.
cord-288669-46tkedw7	131	1	Lane	lane	NN
cord-288669-46tkedw7	131	2	1	1	CD
cord-288669-46tkedw7	131	3	,	,	,
cord-288669-46tkedw7	131	4	molecular	molecular	JJ
cord-288669-46tkedw7	131	5	weight	weight	NN
cord-288669-46tkedw7	131	6	marker	marker	NN
cord-288669-46tkedw7	131	7	;	;	:
cord-288669-46tkedw7	131	8	lanes	lane	NNS
cord-288669-46tkedw7	131	9	2	2	CD
cord-288669-46tkedw7	131	10	and	and	CC
cord-288669-46tkedw7	131	11	3	3	CD
cord-288669-46tkedw7	131	12	,	,	,
cord-288669-46tkedw7	131	13	culture	culture	NN
cord-288669-46tkedw7	131	14	supernatant	supernatant	NN
cord-288669-46tkedw7	131	15	spiked	spike	VBN
cord-288669-46tkedw7	131	16	with	with	IN
cord-288669-46tkedw7	131	17	the	the	DT
cord-288669-46tkedw7	131	18	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	131	19	full	full	JJ
cord-288669-46tkedw7	131	20	-	-	HYPH
cord-288669-46tkedw7	131	21	length	length	NN
cord-288669-46tkedw7	131	22	plasmid	plasmid	NN
cord-288669-46tkedw7	131	23	;	;	:
cord-288669-46tkedw7	131	24	lanes	lane	NNS
cord-288669-46tkedw7	131	25	4	4	CD
cord-288669-46tkedw7	131	26	and	and	CC
cord-288669-46tkedw7	131	27	5	5	CD
cord-288669-46tkedw7	131	28	,	,	,
cord-288669-46tkedw7	131	29	supernatant	supernatant	VBN
cord-288669-46tkedw7	131	30	from	from	IN
cord-288669-46tkedw7	131	31	P129-WT	p129-wt	CD
cord-288669-46tkedw7	131	32	-	-	HYPH
cord-288669-46tkedw7	131	33	transfected	transfecte	VBN
cord-288669-46tkedw7	131	34	cells	cell	NNS
cord-288669-46tkedw7	131	35	;	;	:
cord-288669-46tkedw7	131	36	lanes	lane	NNS
cord-288669-46tkedw7	131	37	6	6	CD
cord-288669-46tkedw7	131	38	and	and	CC
cord-288669-46tkedw7	131	39	7	7	CD
cord-288669-46tkedw7	131	40	,	,	,
cord-288669-46tkedw7	131	41	culture	culture	NN
cord-288669-46tkedw7	131	42	supernatant	supernatant	VBN
cord-288669-46tkedw7	131	43	from	from	IN
cord-288669-46tkedw7	131	44	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	131	45	-	-	HYPH
cord-288669-46tkedw7	131	46	transfected	transfecte	VBN
cord-288669-46tkedw7	131	47	cells	cell	NNS
cord-288669-46tkedw7	131	48	.	.	.
cord-288669-46tkedw7	132	1	(	(	-LRB-
cord-288669-46tkedw7	132	2	C	C	NNP
cord-288669-46tkedw7	132	3	)	)	-RRB-
cord-288669-46tkedw7	132	4	Electron	electron	NN
cord-288669-46tkedw7	132	5	microscopy	microscopy	NN
cord-288669-46tkedw7	132	6	of	of	IN
cord-288669-46tkedw7	132	7	the	the	DT
cord-288669-46tkedw7	132	8	culture	culture	NN
cord-288669-46tkedw7	132	9	supernatant	supernatant	VBN
cord-288669-46tkedw7	132	10	from	from	IN
cord-288669-46tkedw7	132	11	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	132	12	-	-	HYPH
cord-288669-46tkedw7	132	13	transfected	transfecte	VBN
cord-288669-46tkedw7	132	14	cells	cell	NNS
cord-288669-46tkedw7	132	15	.	.	.
cord-288669-46tkedw7	133	1	Particles	particle	NNS
cord-288669-46tkedw7	133	2	in	in	IN
cord-288669-46tkedw7	133	3	the	the	DT
cord-288669-46tkedw7	133	4	culture	culture	NN
cord-288669-46tkedw7	133	5	supernatants	supernatant	NNS
cord-288669-46tkedw7	133	6	released	release	VBN
cord-288669-46tkedw7	133	7	from	from	IN
cord-288669-46tkedw7	133	8	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	133	9	cells	cell	NNS
cord-288669-46tkedw7	133	10	transfected	transfecte	VBN
cord-288669-46tkedw7	133	11	with	with	IN
cord-288669-46tkedw7	133	12	P129-WT	p129-wt	CD
cord-288669-46tkedw7	133	13	(	(	-LRB-
cord-288669-46tkedw7	133	14	upper	upper	JJ
cord-288669-46tkedw7	133	15	panel	panel	NN
cord-288669-46tkedw7	133	16	)	)	-RRB-
cord-288669-46tkedw7	133	17	or	or	CC
cord-288669-46tkedw7	133	18	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	134	1	(	(	-LRB-
cord-288669-46tkedw7	134	2	lower	low	JJR
cord-288669-46tkedw7	134	3	panel	panel	NN
cord-288669-46tkedw7	134	4	)	)	-RRB-
cord-288669-46tkedw7	134	5	genomic	genomic	JJ
cord-288669-46tkedw7	134	6	clones	clone	NNS
cord-288669-46tkedw7	134	7	were	be	VBD
cord-288669-46tkedw7	134	8	concentrated	concentrate	VBN
cord-288669-46tkedw7	134	9	by	by	IN
cord-288669-46tkedw7	134	10	ultracentrifugation	ultracentrifugation	NN
cord-288669-46tkedw7	134	11	through	through	IN
cord-288669-46tkedw7	134	12	a	a	DT
cord-288669-46tkedw7	134	13	20	20	CD
cord-288669-46tkedw7	134	14	%	%	NN
cord-288669-46tkedw7	134	15	(	(	-LRB-
cord-288669-46tkedw7	134	16	wt	wt	NN
cord-288669-46tkedw7	134	17	/	/	SYM
cord-288669-46tkedw7	134	18	vol	vol	NN
cord-288669-46tkedw7	134	19	)	)	-RRB-
cord-288669-46tkedw7	135	1	sucrose	sucrose	NN
cord-288669-46tkedw7	135	2	cushion	cushion	NN
cord-288669-46tkedw7	135	3	.	.	.
cord-288669-46tkedw7	136	1	Pellets	pellet	NNS
cord-288669-46tkedw7	136	2	were	be	VBD
cord-288669-46tkedw7	136	3	negatively	negatively	RB
cord-288669-46tkedw7	136	4	stained	stain	VBN
cord-288669-46tkedw7	136	5	by	by	IN
cord-288669-46tkedw7	136	6	sodium	sodium	NN
cord-288669-46tkedw7	136	7	phosphotungstate	phosphotungstate	NN
cord-288669-46tkedw7	136	8	and	and	CC
cord-288669-46tkedw7	136	9	visualized	visualize	VBN
cord-288669-46tkedw7	136	10	by	by	IN
cord-288669-46tkedw7	136	11	electron	electron	NN
cord-288669-46tkedw7	136	12	microscopy	microscopy	NN
cord-288669-46tkedw7	136	13	.	.	.
cord-288669-46tkedw7	137	1	Scale	scale	NN
cord-288669-46tkedw7	137	2	bar	bar	NN
cord-288669-46tkedw7	137	3	,	,	,
cord-288669-46tkedw7	137	4	100	100	CD
cord-288669-46tkedw7	137	5	nm	nm	NN
cord-288669-46tkedw7	137	6	.	.	NNP
cord-288669-46tkedw7	137	7	suggests	suggest	VBZ
cord-288669-46tkedw7	137	8	that	that	IN
cord-288669-46tkedw7	137	9	the	the	DT
cord-288669-46tkedw7	137	10	lack	lack	NN
cord-288669-46tkedw7	137	11	of	of	IN
cord-288669-46tkedw7	137	12	infectivity	infectivity	NN
cord-288669-46tkedw7	137	13	of	of	IN
cord-288669-46tkedw7	137	14	P129-ΔE	P129-ΔE	NNP
cord-288669-46tkedw7	137	15	was	be	VBD
cord-288669-46tkedw7	137	16	not	not	RB
cord-288669-46tkedw7	137	17	due	due	JJ
cord-288669-46tkedw7	137	18	to	to	IN
cord-288669-46tkedw7	137	19	the	the	DT
cord-288669-46tkedw7	137	20	improper	improper	JJ
cord-288669-46tkedw7	137	21	packaging	packaging	NN
cord-288669-46tkedw7	137	22	of	of	IN
cord-288669-46tkedw7	137	23	the	the	DT
cord-288669-46tkedw7	137	24	genome	genome	NN
cord-288669-46tkedw7	137	25	.	.	.
cord-288669-46tkedw7	138	1	To	to	TO
cord-288669-46tkedw7	138	2	obtain	obtain	VB
cord-288669-46tkedw7	138	3	further	further	JJ
cord-288669-46tkedw7	138	4	evidence	evidence	NN
cord-288669-46tkedw7	138	5	for	for	IN
cord-288669-46tkedw7	138	6	particle	particle	NN
cord-288669-46tkedw7	138	7	formation	formation	NN
cord-288669-46tkedw7	138	8	in	in	IN
cord-288669-46tkedw7	138	9	the	the	DT
cord-288669-46tkedw7	138	10	absence	absence	NN
cord-288669-46tkedw7	138	11	of	of	IN
cord-288669-46tkedw7	138	12	E	e	NN
cord-288669-46tkedw7	138	13	,	,	,
cord-288669-46tkedw7	138	14	electron	electron	NN
cord-288669-46tkedw7	138	15	microscopy	microscopy	NN
cord-288669-46tkedw7	138	16	(	(	-LRB-
cord-288669-46tkedw7	138	17	EM	EM	NNP
cord-288669-46tkedw7	138	18	)	)	-RRB-
cord-288669-46tkedw7	138	19	was	be	VBD
cord-288669-46tkedw7	138	20	conducted	conduct	VBN
cord-288669-46tkedw7	138	21	and	and	CC
cord-288669-46tkedw7	138	22	the	the	DT
cord-288669-46tkedw7	138	23	microscopic	microscopic	JJ
cord-288669-46tkedw7	138	24	appearance	appearance	NN
cord-288669-46tkedw7	138	25	of	of	IN
cord-288669-46tkedw7	138	26	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	138	27	particles	particle	NNS
cord-288669-46tkedw7	138	28	was	be	VBD
cord-288669-46tkedw7	138	29	compared	compare	VBN
cord-288669-46tkedw7	138	30	to	to	IN
cord-288669-46tkedw7	138	31	that	that	DT
cord-288669-46tkedw7	138	32	of	of	IN
cord-288669-46tkedw7	138	33	P129-WT	p129-wt	CD
cord-288669-46tkedw7	138	34	virions	virion	NNS
cord-288669-46tkedw7	138	35	.	.	.
cord-288669-46tkedw7	139	1	At	at	IN
cord-288669-46tkedw7	139	2	2	2	CD
cord-288669-46tkedw7	139	3	days	day	NNS
cord-288669-46tkedw7	139	4	post	post	NN
cord-288669-46tkedw7	139	5	-	-	NN
cord-288669-46tkedw7	139	6	transfection	transfection	JJ
cord-288669-46tkedw7	139	7	,	,	,
cord-288669-46tkedw7	139	8	the	the	DT
cord-288669-46tkedw7	139	9	culture	culture	NN
cord-288669-46tkedw7	139	10	supernatant	supernatant	VBN
cord-288669-46tkedw7	139	11	from	from	IN
cord-288669-46tkedw7	139	12	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	139	13	cells	cell	NNS
cord-288669-46tkedw7	139	14	was	be	VBD
cord-288669-46tkedw7	139	15	harvested	harvest	VBN
cord-288669-46tkedw7	139	16	and	and	CC
cord-288669-46tkedw7	139	17	pelleted	pellete	VBN
cord-288669-46tkedw7	139	18	by	by	IN
cord-288669-46tkedw7	139	19	ultracentrifugation	ultracentrifugation	NN
cord-288669-46tkedw7	139	20	through	through	IN
cord-288669-46tkedw7	139	21	a	a	DT
cord-288669-46tkedw7	139	22	20	20	CD
cord-288669-46tkedw7	139	23	%	%	NN
cord-288669-46tkedw7	139	24	(	(	-LRB-
cord-288669-46tkedw7	139	25	wt	wt	NN
cord-288669-46tkedw7	139	26	/	/	SYM
cord-288669-46tkedw7	139	27	vol	vol	NN
cord-288669-46tkedw7	139	28	)	)	-RRB-
cord-288669-46tkedw7	140	1	sucrose	sucrose	NN
cord-288669-46tkedw7	140	2	cushion	cushion	NN
cord-288669-46tkedw7	140	3	,	,	,
cord-288669-46tkedw7	140	4	followed	follow	VBN
cord-288669-46tkedw7	140	5	by	by	IN
cord-288669-46tkedw7	140	6	electron	electron	NN
cord-288669-46tkedw7	140	7	microscopy	microscopy	NN
cord-288669-46tkedw7	140	8	.	.	.
cord-288669-46tkedw7	141	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	141	2	particles	particle	NNS
cord-288669-46tkedw7	141	3	were	be	VBD
cord-288669-46tkedw7	141	4	identified	identify	VBN
cord-288669-46tkedw7	141	5	in	in	IN
cord-288669-46tkedw7	141	6	the	the	DT
cord-288669-46tkedw7	141	7	supernatant	supernatant	NN
cord-288669-46tkedw7	141	8	of	of	IN
cord-288669-46tkedw7	141	9	cells	cell	NNS
cord-288669-46tkedw7	141	10	transfected	transfecte	VBN
cord-288669-46tkedw7	141	11	with	with	IN
cord-288669-46tkedw7	141	12	either	either	DT
cord-288669-46tkedw7	141	13	P129-WT	p129-wt	CD
cord-288669-46tkedw7	142	1	(	(	-LRB-
cord-288669-46tkedw7	142	2	Fig	Fig	NNP
cord-288669-46tkedw7	142	3	.	.	.
cord-288669-46tkedw7	142	4	3C	3C	NNP
cord-288669-46tkedw7	142	5	,	,	,
cord-288669-46tkedw7	142	6	upper	upper	JJ
cord-288669-46tkedw7	142	7	panel	panel	NN
cord-288669-46tkedw7	142	8	)	)	-RRB-
cord-288669-46tkedw7	142	9	or	or	CC
cord-288669-46tkedw7	142	10	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	143	1	(	(	-LRB-
cord-288669-46tkedw7	143	2	Fig	Fig	NNP
cord-288669-46tkedw7	143	3	.	.	.
cord-288669-46tkedw7	143	4	3C	3C	NNP
cord-288669-46tkedw7	143	5	,	,	,
cord-288669-46tkedw7	143	6	lower	low	JJR
cord-288669-46tkedw7	143	7	panel	panel	NN
cord-288669-46tkedw7	143	8	)	)	-RRB-
cord-288669-46tkedw7	143	9	.	.	.
cord-288669-46tkedw7	144	1	No	no	DT
cord-288669-46tkedw7	144	2	significant	significant	JJ
cord-288669-46tkedw7	144	3	morphologic	morphologic	JJ
cord-288669-46tkedw7	144	4	differences	difference	NNS
cord-288669-46tkedw7	144	5	between	between	IN
cord-288669-46tkedw7	144	6	P129-WT	p129-wt	CD
cord-288669-46tkedw7	144	7	and	and	CC
cord-288669-46tkedw7	144	8	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	144	9	particles	particle	NNS
cord-288669-46tkedw7	144	10	were	be	VBD
cord-288669-46tkedw7	144	11	noted	note	VBN
cord-288669-46tkedw7	144	12	.	.	.
cord-288669-46tkedw7	145	1	Each	each	DT
cord-288669-46tkedw7	145	2	virion	virion	NN
cord-288669-46tkedw7	145	3	for	for	IN
cord-288669-46tkedw7	145	4	P129-WT	p129-wt	CD
cord-288669-46tkedw7	145	5	or	or	CC
cord-288669-46tkedw7	145	6	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	145	7	was	be	VBD
cord-288669-46tkedw7	145	8	a	a	DT
cord-288669-46tkedw7	145	9	roughly	roughly	RB
cord-288669-46tkedw7	145	10	spherical	spherical	JJ
cord-288669-46tkedw7	145	11	enveloped	envelop	VBN
cord-288669-46tkedw7	145	12	particle	particle	NN
cord-288669-46tkedw7	145	13	of	of	IN
cord-288669-46tkedw7	145	14	50	50	CD
cord-288669-46tkedw7	145	15	-	-	SYM
cord-288669-46tkedw7	145	16	60	60	CD
cord-288669-46tkedw7	145	17	nm	nm	NN
cord-288669-46tkedw7	145	18	in	in	IN
cord-288669-46tkedw7	145	19	diameter	diameter	NN
cord-288669-46tkedw7	145	20	,	,	,
cord-288669-46tkedw7	145	21	with	with	IN
cord-288669-46tkedw7	145	22	a	a	DT
cord-288669-46tkedw7	145	23	densely	densely	RB
cord-288669-46tkedw7	145	24	stained	stain	VBN
cord-288669-46tkedw7	145	25	core	core	NN
cord-288669-46tkedw7	145	26	.	.	.
cord-288669-46tkedw7	146	1	The	the	DT
cord-288669-46tkedw7	146	2	EM	EM	NNP
cord-288669-46tkedw7	146	3	study	study	NN
cord-288669-46tkedw7	146	4	confirmed	confirm	VBD
cord-288669-46tkedw7	146	5	that	that	IN
cord-288669-46tkedw7	146	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	146	7	particles	particle	NNS
cord-288669-46tkedw7	146	8	may	may	MD
cord-288669-46tkedw7	146	9	be	be	VB
cord-288669-46tkedw7	146	10	formed	form	VBN
cord-288669-46tkedw7	146	11	in	in	IN
cord-288669-46tkedw7	146	12	the	the	DT
cord-288669-46tkedw7	146	13	absence	absence	NN
cord-288669-46tkedw7	146	14	of	of	IN
cord-288669-46tkedw7	146	15	E	e	NN
cord-288669-46tkedw7	146	16	protein	protein	NN
cord-288669-46tkedw7	146	17	and	and	CC
cord-288669-46tkedw7	146	18	also	also	RB
cord-288669-46tkedw7	146	19	showed	show	VBD
cord-288669-46tkedw7	146	20	that	that	IN
cord-288669-46tkedw7	146	21	the	the	DT
cord-288669-46tkedw7	146	22	E	e	NN
cord-288669-46tkedw7	146	23	proteinnegative	proteinnegative	NN
cord-288669-46tkedw7	146	24	particles	particle	NNS
cord-288669-46tkedw7	146	25	had	have	VBD
cord-288669-46tkedw7	146	26	similar	similar	JJ
cord-288669-46tkedw7	146	27	appearance	appearance	NN
cord-288669-46tkedw7	146	28	and	and	CC
cord-288669-46tkedw7	146	29	size	size	NN
cord-288669-46tkedw7	146	30	to	to	IN
cord-288669-46tkedw7	146	31	wild	wild	JJ
cord-288669-46tkedw7	146	32	-	-	HYPH
cord-288669-46tkedw7	146	33	type	type	NN
cord-288669-46tkedw7	146	34	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	146	35	particles	particle	NNS
cord-288669-46tkedw7	146	36	.	.	.
cord-288669-46tkedw7	147	1	To	to	TO
cord-288669-46tkedw7	147	2	determine	determine	VB
cord-288669-46tkedw7	147	3	if	if	IN
cord-288669-46tkedw7	147	4	the	the	DT
cord-288669-46tkedw7	147	5	lack	lack	NN
cord-288669-46tkedw7	147	6	of	of	IN
cord-288669-46tkedw7	147	7	infectivity	infectivity	NN
cord-288669-46tkedw7	147	8	of	of	IN
cord-288669-46tkedw7	147	9	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	147	10	particles	particle	NNS
cord-288669-46tkedw7	147	11	was	be	VBD
cord-288669-46tkedw7	147	12	due	due	JJ
cord-288669-46tkedw7	147	13	to	to	IN
cord-288669-46tkedw7	147	14	a	a	DT
cord-288669-46tkedw7	147	15	low	low	JJ
cord-288669-46tkedw7	147	16	amount	amount	NN
cord-288669-46tkedw7	147	17	of	of	IN
cord-288669-46tkedw7	147	18	virus	virus	NN
cord-288669-46tkedw7	147	19	produced	produce	VBN
cord-288669-46tkedw7	147	20	from	from	IN
cord-288669-46tkedw7	147	21	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	147	22	cells	cell	NNS
cord-288669-46tkedw7	147	23	,	,	,
cord-288669-46tkedw7	147	24	the	the	DT
cord-288669-46tkedw7	147	25	supernatants	supernatant	NNS
cord-288669-46tkedw7	147	26	collected	collect	VBN
cord-288669-46tkedw7	147	27	from	from	IN
cord-288669-46tkedw7	147	28	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	147	29	cells	cell	NNS
cord-288669-46tkedw7	147	30	were	be	VBD
cord-288669-46tkedw7	147	31	blindly	blindly	RB
cord-288669-46tkedw7	147	32	passaged	passage	VBN
cord-288669-46tkedw7	147	33	twice	twice	RB
cord-288669-46tkedw7	147	34	in	in	IN
cord-288669-46tkedw7	147	35	Marc-145	marc-145	DT
cord-288669-46tkedw7	147	36	cells	cell	NNS
cord-288669-46tkedw7	147	37	to	to	TO
cord-288669-46tkedw7	147	38	amplify	amplify	VB
cord-288669-46tkedw7	147	39	the	the	DT
cord-288669-46tkedw7	147	40	infectivity	infectivity	NN
cord-288669-46tkedw7	147	41	.	.	.
cord-288669-46tkedw7	148	1	Although	although	IN
cord-288669-46tkedw7	148	2	extensive	extensive	JJ
cord-288669-46tkedw7	148	3	CPE	CPE	NNP
cord-288669-46tkedw7	148	4	was	be	VBD
cord-288669-46tkedw7	148	5	readily	readily	RB
cord-288669-46tkedw7	148	6	evident	evident	JJ
cord-288669-46tkedw7	148	7	in	in	IN
cord-288669-46tkedw7	148	8	Marc-145	marc-145	DT
cord-288669-46tkedw7	148	9	cells	cell	NNS
cord-288669-46tkedw7	148	10	inoculated	inoculate	VBN
cord-288669-46tkedw7	148	11	with	with	IN
cord-288669-46tkedw7	148	12	either	either	CC
cord-288669-46tkedw7	148	13	passage-1	passage-1	NNP
cord-288669-46tkedw7	148	14	or	or	CC
cord-288669-46tkedw7	148	15	passage-2	passage-2	NNP
cord-288669-46tkedw7	148	16	of	of	IN
cord-288669-46tkedw7	148	17	P129-WT	p129-wt	CD
cord-288669-46tkedw7	148	18	supernatant	supernatant	NN
cord-288669-46tkedw7	148	19	,	,	,
cord-288669-46tkedw7	148	20	no	no	DT
cord-288669-46tkedw7	148	21	CPE	CPE	NNP
cord-288669-46tkedw7	148	22	was	be	VBD
cord-288669-46tkedw7	148	23	detectable	detectable	JJ
cord-288669-46tkedw7	148	24	with	with	IN
cord-288669-46tkedw7	148	25	passage-1	passage-1	NNP
cord-288669-46tkedw7	148	26	or	or	CC
cord-288669-46tkedw7	148	27	passage-2	passage-2	NNP
cord-288669-46tkedw7	148	28	of	of	IN
cord-288669-46tkedw7	148	29	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	148	30	supernatant	supernatant	NN
cord-288669-46tkedw7	148	31	,	,	,
cord-288669-46tkedw7	148	32	even	even	RB
cord-288669-46tkedw7	148	33	after	after	IN
cord-288669-46tkedw7	148	34	5	5	CD
cord-288669-46tkedw7	148	35	days	day	NNS
cord-288669-46tkedw7	148	36	postinoculation	postinoculation	NN
cord-288669-46tkedw7	148	37	(	(	-LRB-
cord-288669-46tkedw7	148	38	data	datum	NNS
cord-288669-46tkedw7	148	39	not	not	RB
cord-288669-46tkedw7	148	40	shown	show	VBN
cord-288669-46tkedw7	148	41	)	)	-RRB-
cord-288669-46tkedw7	148	42	.	.	.
cord-288669-46tkedw7	149	1	To	to	TO
cord-288669-46tkedw7	149	2	further	further	RB
cord-288669-46tkedw7	149	3	determine	determine	VB
cord-288669-46tkedw7	149	4	infectivity	infectivity	NN
cord-288669-46tkedw7	149	5	in	in	IN
cord-288669-46tkedw7	149	6	Marc-145	marc-145	CD
cord-288669-46tkedw7	149	7	cells	cell	NNS
cord-288669-46tkedw7	149	8	inoculated	inoculate	VBN
cord-288669-46tkedw7	149	9	with	with	IN
cord-288669-46tkedw7	149	10	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	149	11	virus	virus	NN
cord-288669-46tkedw7	149	12	,	,	,
cord-288669-46tkedw7	149	13	time	time	NN
cord-288669-46tkedw7	149	14	course	course	NN
cord-288669-46tkedw7	149	15	immunofluorescence	immunofluorescence	NN
cord-288669-46tkedw7	149	16	was	be	VBD
cord-288669-46tkedw7	149	17	carried	carry	VBN
cord-288669-46tkedw7	149	18	out	out	RP
cord-288669-46tkedw7	149	19	with	with	IN
cord-288669-46tkedw7	149	20	the	the	DT
cord-288669-46tkedw7	149	21	N	n	CD
cord-288669-46tkedw7	149	22	-	-	HYPH
cord-288669-46tkedw7	149	23	specific	specific	JJ
cord-288669-46tkedw7	149	24	antibody	antibody	NN
cord-288669-46tkedw7	149	25	.	.	.
cord-288669-46tkedw7	150	1	As	as	IN
cord-288669-46tkedw7	150	2	shown	show	VBN
cord-288669-46tkedw7	150	3	in	in	IN
cord-288669-46tkedw7	150	4	Fig	Fig	NNP
cord-288669-46tkedw7	150	5	.	.	.
cord-288669-46tkedw7	150	6	4A	4a	CD
cord-288669-46tkedw7	150	7	,	,	,
cord-288669-46tkedw7	150	8	distinct	distinct	JJ
cord-288669-46tkedw7	150	9	staining	staining	NN
cord-288669-46tkedw7	150	10	of	of	IN
cord-288669-46tkedw7	150	11	N	N	NNP
cord-288669-46tkedw7	150	12	was	be	VBD
cord-288669-46tkedw7	150	13	first	first	RB
cord-288669-46tkedw7	150	14	observed	observe	VBN
cord-288669-46tkedw7	150	15	at	at	IN
cord-288669-46tkedw7	150	16	12	12	CD
cord-288669-46tkedw7	150	17	h	h	NN
cord-288669-46tkedw7	150	18	post	post	NN
cord-288669-46tkedw7	150	19	-	-	NN
cord-288669-46tkedw7	150	20	infection	infection	NN
cord-288669-46tkedw7	150	21	in	in	IN
cord-288669-46tkedw7	150	22	Marc-145	marc-145	CD
cord-288669-46tkedw7	150	23	cells	cell	NNS
cord-288669-46tkedw7	150	24	inoculated	inoculate	VBN
cord-288669-46tkedw7	150	25	with	with	IN
cord-288669-46tkedw7	150	26	P129-WT	p129-wt	CD
cord-288669-46tkedw7	150	27	supernatant	supernatant	NN
cord-288669-46tkedw7	150	28	,	,	,
cord-288669-46tkedw7	150	29	and	and	CC
cord-288669-46tkedw7	150	30	then	then	RB
cord-288669-46tkedw7	150	31	in	in	IN
cord-288669-46tkedw7	150	32	many	many	JJ
cord-288669-46tkedw7	150	33	clusters	cluster	NNS
cord-288669-46tkedw7	150	34	of	of	IN
cord-288669-46tkedw7	150	35	cells	cell	NNS
cord-288669-46tkedw7	150	36	by	by	IN
cord-288669-46tkedw7	150	37	48	48	CD
cord-288669-46tkedw7	150	38	h	h	NN
cord-288669-46tkedw7	150	39	post	post	NN
cord-288669-46tkedw7	150	40	-	-	NN
cord-288669-46tkedw7	150	41	infection	infection	NN
cord-288669-46tkedw7	150	42	,	,	,
cord-288669-46tkedw7	150	43	showing	show	VBG
cord-288669-46tkedw7	150	44	the	the	DT
cord-288669-46tkedw7	150	45	spread	spread	NN
cord-288669-46tkedw7	150	46	of	of	IN
cord-288669-46tkedw7	150	47	infectivity	infectivity	NN
cord-288669-46tkedw7	150	48	to	to	IN
cord-288669-46tkedw7	150	49	neighboring	neighboring	NN
cord-288669-46tkedw7	150	50	cells	cell	NNS
cord-288669-46tkedw7	150	51	(	(	-LRB-
cord-288669-46tkedw7	150	52	upper	upper	JJ
cord-288669-46tkedw7	150	53	panels	panel	NNS
cord-288669-46tkedw7	150	54	)	)	-RRB-
cord-288669-46tkedw7	150	55	.	.	.
cord-288669-46tkedw7	151	1	In	in	IN
cord-288669-46tkedw7	151	2	contrast	contrast	NN
cord-288669-46tkedw7	151	3	,	,	,
cord-288669-46tkedw7	151	4	cells	cell	NNS
cord-288669-46tkedw7	151	5	inoculated	inoculate	VBN
cord-288669-46tkedw7	151	6	with	with	IN
cord-288669-46tkedw7	151	7	passage-1	passage-1	NNP
cord-288669-46tkedw7	151	8	of	of	IN
cord-288669-46tkedw7	151	9	P129-ΔE	P129-ΔE	NNP
cord-288669-46tkedw7	151	10	showed	show	VBD
cord-288669-46tkedw7	151	11	no	no	DT
cord-288669-46tkedw7	151	12	specific	specific	JJ
cord-288669-46tkedw7	151	13	staining	staining	NN
cord-288669-46tkedw7	151	14	throughout	throughout	IN
cord-288669-46tkedw7	151	15	the	the	DT
cord-288669-46tkedw7	151	16	experiments	experiment	NNS
cord-288669-46tkedw7	151	17	,	,	,
cord-288669-46tkedw7	151	18	further	further	RB
cord-288669-46tkedw7	151	19	indicating	indicate	VBG
cord-288669-46tkedw7	151	20	the	the	DT
cord-288669-46tkedw7	151	21	lack	lack	NN
cord-288669-46tkedw7	151	22	of	of	IN
cord-288669-46tkedw7	151	23	infectivity	infectivity	NN
cord-288669-46tkedw7	151	24	(	(	-LRB-
cord-288669-46tkedw7	151	25	lower	low	JJR
cord-288669-46tkedw7	151	26	panels	panel	NNS
cord-288669-46tkedw7	151	27	)	)	-RRB-
cord-288669-46tkedw7	151	28	.	.	.
cord-288669-46tkedw7	152	1	Because	because	IN
cord-288669-46tkedw7	152	2	neither	neither	CC
cord-288669-46tkedw7	152	3	CPE	CPE	NNP
cord-288669-46tkedw7	152	4	nor	nor	CC
cord-288669-46tkedw7	152	5	N	n	CD
cord-288669-46tkedw7	152	6	-	-	HYPH
cord-288669-46tkedw7	152	7	specific	specific	JJ
cord-288669-46tkedw7	152	8	staining	staining	NN
cord-288669-46tkedw7	152	9	was	be	VBD
cord-288669-46tkedw7	152	10	detectable	detectable	JJ
cord-288669-46tkedw7	152	11	from	from	IN
cord-288669-46tkedw7	152	12	serial	serial	JJ
cord-288669-46tkedw7	152	13	passages	passage	NNS
cord-288669-46tkedw7	152	14	of	of	IN
cord-288669-46tkedw7	152	15	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	152	16	virus	virus	NN
cord-288669-46tkedw7	152	17	,	,	,
cord-288669-46tkedw7	152	18	RT	RT	NNP
cord-288669-46tkedw7	152	19	-	-	HYPH
cord-288669-46tkedw7	152	20	PCR	PCR	NNP
cord-288669-46tkedw7	152	21	was	be	VBD
cord-288669-46tkedw7	152	22	conducted	conduct	VBN
cord-288669-46tkedw7	152	23	from	from	IN
cord-288669-46tkedw7	152	24	cells	cell	NNS
cord-288669-46tkedw7	152	25	and	and	CC
cord-288669-46tkedw7	152	26	culture	culture	NN
cord-288669-46tkedw7	152	27	supernatants	supernatant	NNS
cord-288669-46tkedw7	152	28	inoculated	inoculate	VBN
cord-288669-46tkedw7	152	29	with	with	IN
cord-288669-46tkedw7	152	30	passage-1	passage-1	NNP
cord-288669-46tkedw7	152	31	or	or	CC
cord-288669-46tkedw7	152	32	passage-2	passage-2	NNP
cord-288669-46tkedw7	153	1	(	(	-LRB-
cord-288669-46tkedw7	153	2	Fig	Fig	NNP
cord-288669-46tkedw7	153	3	.	.	.
cord-288669-46tkedw7	153	4	4B	4b	CD
cord-288669-46tkedw7	153	5	)	)	-RRB-
cord-288669-46tkedw7	153	6	.	.	.
cord-288669-46tkedw7	154	1	To	to	TO
cord-288669-46tkedw7	154	2	eliminate	eliminate	VB
cord-288669-46tkedw7	154	3	a	a	DT
cord-288669-46tkedw7	154	4	possible	possible	JJ
cord-288669-46tkedw7	154	5	carry	carry	NN
cord-288669-46tkedw7	154	6	-	-	HYPH
cord-288669-46tkedw7	154	7	over	over	RP
cord-288669-46tkedw7	154	8	contamination	contamination	NN
cord-288669-46tkedw7	154	9	of	of	IN
cord-288669-46tkedw7	154	10	the	the	DT
cord-288669-46tkedw7	154	11	transfected	transfecte	VBN
cord-288669-46tkedw7	154	12	DNA	DNA	NNP
cord-288669-46tkedw7	154	13	,	,	,
cord-288669-46tkedw7	154	14	the	the	DT
cord-288669-46tkedw7	154	15	RNA	RNA	NNP
cord-288669-46tkedw7	154	16	preparations	preparation	NNS
cord-288669-46tkedw7	154	17	were	be	VBD
cord-288669-46tkedw7	154	18	treated	treat	VBN
cord-288669-46tkedw7	154	19	with	with	IN
cord-288669-46tkedw7	154	20	RNase	rnase	NN
cord-288669-46tkedw7	154	21	-	-	HYPH
cord-288669-46tkedw7	154	22	free	free	JJ
cord-288669-46tkedw7	154	23	DNase	dnase	XX
cord-288669-46tkedw7	155	1	I	-PRON-	PRP
cord-288669-46tkedw7	155	2	prior	prior	RB
cord-288669-46tkedw7	155	3	to	to	IN
cord-288669-46tkedw7	155	4	the	the	DT
cord-288669-46tkedw7	155	5	E	e	NN
cord-288669-46tkedw7	155	6	gene	gene	NN
cord-288669-46tkedw7	155	7	amplification	amplification	NN
cord-288669-46tkedw7	155	8	.	.	.
cord-288669-46tkedw7	156	1	A	a	DT
cord-288669-46tkedw7	156	2	260-bp	260-bp	CD
cord-288669-46tkedw7	156	3	product	product	NN
cord-288669-46tkedw7	156	4	was	be	VBD
cord-288669-46tkedw7	156	5	amplified	amplify	VBN
cord-288669-46tkedw7	156	6	from	from	IN
cord-288669-46tkedw7	156	7	both	both	DT
cord-288669-46tkedw7	156	8	culture	culture	NN
cord-288669-46tkedw7	156	9	media	medium	NNS
cord-288669-46tkedw7	156	10	and	and	CC
cord-288669-46tkedw7	156	11	cells	cell	NNS
cord-288669-46tkedw7	156	12	that	that	WDT
cord-288669-46tkedw7	156	13	were	be	VBD
cord-288669-46tkedw7	156	14	inoculated	inoculate	VBN
cord-288669-46tkedw7	156	15	with	with	IN
cord-288669-46tkedw7	156	16	passage-1	passage-1	NNP
cord-288669-46tkedw7	156	17	or	or	CC
cord-288669-46tkedw7	156	18	passage-2	passage-2	NNP
cord-288669-46tkedw7	156	19	P129-WT	p129-wt	CD
cord-288669-46tkedw7	156	20	virus	virus	NN
cord-288669-46tkedw7	157	1	(	(	-LRB-
cord-288669-46tkedw7	157	2	Fig	Fig	NNP
cord-288669-46tkedw7	157	3	.	.	.
cord-288669-46tkedw7	157	4	4B	4b	CD
cord-288669-46tkedw7	157	5	,	,	,
cord-288669-46tkedw7	157	6	lanes	lane	VBZ
cord-288669-46tkedw7	157	7	2	2	CD
cord-288669-46tkedw7	157	8	,	,	,
cord-288669-46tkedw7	157	9	5	5	CD
cord-288669-46tkedw7	157	10	,	,	,
cord-288669-46tkedw7	157	11	8	8	CD
cord-288669-46tkedw7	157	12	and	and	CC
cord-288669-46tkedw7	157	13	11	11	CD
cord-288669-46tkedw7	157	14	)	)	-RRB-
cord-288669-46tkedw7	157	15	.	.	.
cord-288669-46tkedw7	158	1	The	the	DT
cord-288669-46tkedw7	158	2	260-bp	260-bp	CD
cord-288669-46tkedw7	158	3	product	product	NN
cord-288669-46tkedw7	158	4	was	be	VBD
cord-288669-46tkedw7	158	5	also	also	RB
cord-288669-46tkedw7	158	6	obtainable	obtainable	JJ
cord-288669-46tkedw7	158	7	from	from	IN
cord-288669-46tkedw7	158	8	cells	cell	NNS
cord-288669-46tkedw7	158	9	inoculated	inoculate	VBN
cord-288669-46tkedw7	158	10	with	with	IN
cord-288669-46tkedw7	158	11	passage-1	passage-1	NNP
cord-288669-46tkedw7	158	12	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	158	13	virus	virus	NN
cord-288669-46tkedw7	158	14	(	(	-LRB-
cord-288669-46tkedw7	158	15	Fig	Fig	NNP
cord-288669-46tkedw7	158	16	.	.	.
cord-288669-46tkedw7	158	17	4B	4b	CD
cord-288669-46tkedw7	158	18	,	,	,
cord-288669-46tkedw7	158	19	lane	lane	NN
cord-288669-46tkedw7	158	20	3	3	CD
cord-288669-46tkedw7	158	21	)	)	-RRB-
cord-288669-46tkedw7	158	22	,	,	,
cord-288669-46tkedw7	158	23	but	but	CC
cord-288669-46tkedw7	158	24	no	no	DT
cord-288669-46tkedw7	158	25	specific	specific	JJ
cord-288669-46tkedw7	158	26	product	product	NN
cord-288669-46tkedw7	158	27	was	be	VBD
cord-288669-46tkedw7	158	28	amplified	amplify	VBN
cord-288669-46tkedw7	158	29	from	from	IN
cord-288669-46tkedw7	158	30	the	the	DT
cord-288669-46tkedw7	158	31	supernatant	supernatant	NN
cord-288669-46tkedw7	158	32	(	(	-LRB-
cord-288669-46tkedw7	158	33	Fig	Fig	NNP
cord-288669-46tkedw7	158	34	.	.	.
cord-288669-46tkedw7	158	35	4B	4b	CD
cord-288669-46tkedw7	158	36	,	,	,
cord-288669-46tkedw7	158	37	lane	lane	NNP
cord-288669-46tkedw7	158	38	6	6	CD
cord-288669-46tkedw7	158	39	)	)	-RRB-
cord-288669-46tkedw7	158	40	,	,	,
cord-288669-46tkedw7	158	41	nor	nor	CC
cord-288669-46tkedw7	158	42	from	from	IN
cord-288669-46tkedw7	158	43	either	either	DT
cord-288669-46tkedw7	158	44	supernatants	supernatant	NNS
cord-288669-46tkedw7	158	45	or	or	CC
cord-288669-46tkedw7	158	46	cells	cell	NNS
cord-288669-46tkedw7	158	47	inoculated	inoculate	VBN
cord-288669-46tkedw7	158	48	with	with	IN
cord-288669-46tkedw7	158	49	passage-2	passage-2	NNP
cord-288669-46tkedw7	158	50	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	158	51	virus	virus	NN
cord-288669-46tkedw7	158	52	(	(	-LRB-
cord-288669-46tkedw7	158	53	Fig	Fig	NNP
cord-288669-46tkedw7	158	54	.	.	.
cord-288669-46tkedw7	158	55	4B	4b	CD
cord-288669-46tkedw7	158	56	,	,	.
cord-288669-46tkedw7	159	1	lanes	lane	NNS
cord-288669-46tkedw7	159	2	9	9	CD
cord-288669-46tkedw7	159	3	and	and	CC
cord-288669-46tkedw7	159	4	12	12	CD
cord-288669-46tkedw7	159	5	)	)	-RRB-
cord-288669-46tkedw7	159	6	.	.	.
cord-288669-46tkedw7	160	1	The	the	DT
cord-288669-46tkedw7	160	2	amplified	amplified	JJ
cord-288669-46tkedw7	160	3	fragment	fragment	NN
cord-288669-46tkedw7	160	4	was	be	VBD
cord-288669-46tkedw7	160	5	sequenced	sequence	VBN
cord-288669-46tkedw7	160	6	,	,	,
cord-288669-46tkedw7	160	7	and	and	CC
cord-288669-46tkedw7	160	8	the	the	DT
cord-288669-46tkedw7	160	9	sequencing	sequencing	NN
cord-288669-46tkedw7	160	10	results	result	NNS
cord-288669-46tkedw7	160	11	confirmed	confirm	VBD
cord-288669-46tkedw7	160	12	the	the	DT
cord-288669-46tkedw7	160	13	'	'	``
cord-288669-46tkedw7	160	14	A12062	a12062	NN
cord-288669-46tkedw7	160	15	G	g	NN
cord-288669-46tkedw7	160	16	'	'	POS
cord-288669-46tkedw7	160	17	mutation	mutation	NN
cord-288669-46tkedw7	160	18	at	at	IN
cord-288669-46tkedw7	160	19	the	the	DT
cord-288669-46tkedw7	160	20	start	start	NN
cord-288669-46tkedw7	160	21	codon	codon	NN
cord-288669-46tkedw7	160	22	of	of	IN
cord-288669-46tkedw7	160	23	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	161	1	(	(	-LRB-
cord-288669-46tkedw7	161	2	data	datum	NNS
cord-288669-46tkedw7	161	3	not	not	RB
cord-288669-46tkedw7	161	4	shown	show	VBN
cord-288669-46tkedw7	161	5	)	)	-RRB-
cord-288669-46tkedw7	161	6	.	.	.
cord-288669-46tkedw7	162	1	These	these	DT
cord-288669-46tkedw7	162	2	results	result	NNS
cord-288669-46tkedw7	162	3	suggest	suggest	VBP
cord-288669-46tkedw7	162	4	that	that	IN
cord-288669-46tkedw7	162	5	the	the	DT
cord-288669-46tkedw7	162	6	E	e	NN
cord-288669-46tkedw7	162	7	protein	protein	NN
cord-288669-46tkedw7	162	8	-	-	HYPH
cord-288669-46tkedw7	162	9	negative	negative	JJ
cord-288669-46tkedw7	162	10	,	,	,
cord-288669-46tkedw7	162	11	noninfectious	noninfectious	JJ
cord-288669-46tkedw7	162	12	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	162	13	virus	virus	NN
cord-288669-46tkedw7	162	14	particles	particle	NNS
cord-288669-46tkedw7	162	15	entered	enter	VBD
cord-288669-46tkedw7	162	16	Marc-145	marc-145	CD
cord-288669-46tkedw7	162	17	cells	cell	NNS
cord-288669-46tkedw7	162	18	,	,	,
cord-288669-46tkedw7	162	19	but	but	CC
cord-288669-46tkedw7	162	20	further	further	JJ
cord-288669-46tkedw7	162	21	steps	step	NNS
cord-288669-46tkedw7	162	22	beyond	beyond	IN
cord-288669-46tkedw7	162	23	entry	entry	NN
cord-288669-46tkedw7	162	24	were	be	VBD
cord-288669-46tkedw7	162	25	interrupted	interrupt	VBN
cord-288669-46tkedw7	162	26	,	,	,
cord-288669-46tkedw7	162	27	resulting	result	VBG
cord-288669-46tkedw7	162	28	in	in	IN
cord-288669-46tkedw7	162	29	the	the	DT
cord-288669-46tkedw7	162	30	absence	absence	NN
cord-288669-46tkedw7	162	31	of	of	IN
cord-288669-46tkedw7	162	32	infectivity	infectivity	NN
cord-288669-46tkedw7	162	33	.	.	.
cord-288669-46tkedw7	163	1	To	to	TO
cord-288669-46tkedw7	163	2	investigate	investigate	VB
cord-288669-46tkedw7	163	3	whether	whether	IN
cord-288669-46tkedw7	163	4	the	the	DT
cord-288669-46tkedw7	163	5	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	163	6	virus	virus	NN
cord-288669-46tkedw7	163	7	genome	genome	NN
cord-288669-46tkedw7	163	8	underwent	undergo	VBD
cord-288669-46tkedw7	163	9	replication	replication	NN
cord-288669-46tkedw7	163	10	following	follow	VBG
cord-288669-46tkedw7	163	11	entry	entry	NN
cord-288669-46tkedw7	163	12	,	,	,
cord-288669-46tkedw7	163	13	strand	strand	NN
cord-288669-46tkedw7	163	14	-	-	HYPH
cord-288669-46tkedw7	163	15	specific	specific	JJ
cord-288669-46tkedw7	163	16	RT	RT	NNP
cord-288669-46tkedw7	163	17	-	-	HYPH
cord-288669-46tkedw7	163	18	PCR	PCR	NNP
cord-288669-46tkedw7	163	19	was	be	VBD
cord-288669-46tkedw7	163	20	performed	perform	VBN
cord-288669-46tkedw7	163	21	from	from	IN
cord-288669-46tkedw7	163	22	cells	cell	NNS
cord-288669-46tkedw7	163	23	inoculated	inoculate	VBN
cord-288669-46tkedw7	163	24	with	with	IN
cord-288669-46tkedw7	163	25	passage-1	passage-1	NNP
cord-288669-46tkedw7	163	26	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	163	27	virus	virus	NN
cord-288669-46tkedw7	163	28	.	.	.
cord-288669-46tkedw7	164	1	RT	RT	NNP
cord-288669-46tkedw7	164	2	-	-	HYPH
cord-288669-46tkedw7	164	3	PCR	PCR	NNP
cord-288669-46tkedw7	164	4	from	from	IN
cord-288669-46tkedw7	164	5	P129-WT	p129-wt	CD
cord-288669-46tkedw7	164	6	-	-	HYPH
cord-288669-46tkedw7	164	7	inoculated	inoculate	VBN
cord-288669-46tkedw7	164	8	cells	cell	NNS
cord-288669-46tkedw7	164	9	yielded	yield	VBD
cord-288669-46tkedw7	164	10	a	a	DT
cord-288669-46tkedw7	164	11	specific	specific	JJ
cord-288669-46tkedw7	164	12	product	product	NN
cord-288669-46tkedw7	164	13	of	of	IN
cord-288669-46tkedw7	164	14	the	the	DT
cord-288669-46tkedw7	164	15	expected	expect	VBN
cord-288669-46tkedw7	164	16	size	size	NN
cord-288669-46tkedw7	164	17	for	for	IN
cord-288669-46tkedw7	164	18	both	both	DT
cord-288669-46tkedw7	164	19	positive	positive	JJ
cord-288669-46tkedw7	164	20	-	-	HYPH
cord-288669-46tkedw7	164	21	and	and	CC
cord-288669-46tkedw7	164	22	negativestrand	negativestrand	NN
cord-288669-46tkedw7	164	23	genomes	genome	NNS
cord-288669-46tkedw7	164	24	and	and	CC
cord-288669-46tkedw7	164	25	also	also	RB
cord-288669-46tkedw7	164	26	for	for	IN
cord-288669-46tkedw7	164	27	positive	positive	JJ
cord-288669-46tkedw7	164	28	-	-	HYPH
cord-288669-46tkedw7	164	29	and	and	CC
cord-288669-46tkedw7	164	30	negative	negative	JJ
cord-288669-46tkedw7	164	31	-	-	HYPH
cord-288669-46tkedw7	164	32	strand	strand	NN
cord-288669-46tkedw7	164	33	mRNA7	mrna7	NN
cord-288669-46tkedw7	165	1	(	(	-LRB-
cord-288669-46tkedw7	165	2	Fig	Fig	NNP
cord-288669-46tkedw7	165	3	.	.	.
cord-288669-46tkedw7	165	4	4C	4c	CD
cord-288669-46tkedw7	165	5	,	,	,
cord-288669-46tkedw7	165	6	lanes	lane	VBZ
cord-288669-46tkedw7	165	7	3	3	CD
cord-288669-46tkedw7	165	8	in	in	IN
cord-288669-46tkedw7	165	9	all	all	DT
cord-288669-46tkedw7	165	10	panels	panel	NNS
cord-288669-46tkedw7	165	11	)	)	-RRB-
cord-288669-46tkedw7	165	12	.	.	.
cord-288669-46tkedw7	166	1	In	in	IN
cord-288669-46tkedw7	166	2	contrast	contrast	NN
cord-288669-46tkedw7	166	3	,	,	,
cord-288669-46tkedw7	166	4	only	only	RB
cord-288669-46tkedw7	166	5	a	a	DT
cord-288669-46tkedw7	166	6	minimal	minimal	JJ
cord-288669-46tkedw7	166	7	amount	amount	NN
cord-288669-46tkedw7	166	8	was	be	VBD
cord-288669-46tkedw7	166	9	amplified	amplify	VBN
cord-288669-46tkedw7	166	10	for	for	IN
cord-288669-46tkedw7	166	11	the	the	DT
cord-288669-46tkedw7	166	12	positive	positive	JJ
cord-288669-46tkedw7	166	13	-	-	HYPH
cord-288669-46tkedw7	166	14	strand	strand	NN
cord-288669-46tkedw7	166	15	genome	genome	NN
cord-288669-46tkedw7	166	16	from	from	IN
cord-288669-46tkedw7	166	17	cells	cell	NNS
cord-288669-46tkedw7	166	18	inoculated	inoculate	VBN
cord-288669-46tkedw7	166	19	with	with	IN
cord-288669-46tkedw7	166	20	passage-1	passage-1	NNP
cord-288669-46tkedw7	166	21	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	166	22	virus	virus	NN
cord-288669-46tkedw7	167	1	(	(	-LRB-
cord-288669-46tkedw7	167	2	Fig	Fig	NNP
cord-288669-46tkedw7	167	3	.	.	.
cord-288669-46tkedw7	167	4	4C	4c	CD
cord-288669-46tkedw7	167	5	,	,	,
cord-288669-46tkedw7	167	6	panel	panel	NN
cord-288669-46tkedw7	167	7	i	i	PRP
cord-288669-46tkedw7	167	8	,	,	,
cord-288669-46tkedw7	167	9	lane	lane	NN
cord-288669-46tkedw7	167	10	4	4	CD
cord-288669-46tkedw7	167	11	)	)	-RRB-
cord-288669-46tkedw7	167	12	.	.	.
cord-288669-46tkedw7	168	1	This	this	DT
cord-288669-46tkedw7	168	2	product	product	NN
cord-288669-46tkedw7	168	3	was	be	VBD
cord-288669-46tkedw7	168	4	likely	likely	RB
cord-288669-46tkedw7	168	5	derived	derive	VBN
cord-288669-46tkedw7	168	6	from	from	IN
cord-288669-46tkedw7	168	7	the	the	DT
cord-288669-46tkedw7	168	8	incoming	incoming	JJ
cord-288669-46tkedw7	168	9	genomic	genomic	JJ
cord-288669-46tkedw7	168	10	RNA	RNA	NNP
cord-288669-46tkedw7	168	11	of	of	IN
cord-288669-46tkedw7	168	12	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	168	13	virus	virus	NN
cord-288669-46tkedw7	168	14	.	.	.
cord-288669-46tkedw7	169	1	These	these	DT
cord-288669-46tkedw7	169	2	results	result	NNS
cord-288669-46tkedw7	169	3	indicate	indicate	VBP
cord-288669-46tkedw7	169	4	that	that	IN
cord-288669-46tkedw7	169	5	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	169	6	virus	virus	NN
cord-288669-46tkedw7	169	7	was	be	VBD
cord-288669-46tkedw7	169	8	unable	unable	JJ
cord-288669-46tkedw7	169	9	to	to	TO
cord-288669-46tkedw7	169	10	replicate	replicate	VB
cord-288669-46tkedw7	169	11	,	,	,
cord-288669-46tkedw7	169	12	suggesting	suggest	VBG
cord-288669-46tkedw7	169	13	that	that	IN
cord-288669-46tkedw7	169	14	the	the	DT
cord-288669-46tkedw7	169	15	lack	lack	NN
cord-288669-46tkedw7	169	16	of	of	IN
cord-288669-46tkedw7	169	17	RNA	RNA	NNP
cord-288669-46tkedw7	169	18	replication	replication	NN
cord-288669-46tkedw7	169	19	of	of	IN
cord-288669-46tkedw7	169	20	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	169	21	virus	virus	NN
cord-288669-46tkedw7	169	22	was	be	VBD
cord-288669-46tkedw7	169	23	likely	likely	JJ
cord-288669-46tkedw7	169	24	due	due	IN
cord-288669-46tkedw7	169	25	to	to	IN
cord-288669-46tkedw7	169	26	an	an	DT
cord-288669-46tkedw7	169	27	interruption	interruption	NN
cord-288669-46tkedw7	169	28	during	during	IN
cord-288669-46tkedw7	169	29	virus	virus	NN
cord-288669-46tkedw7	169	30	uncoating	uncoating	NN
cord-288669-46tkedw7	169	31	,	,	,
cord-288669-46tkedw7	169	32	a	a	DT
cord-288669-46tkedw7	169	33	stage	stage	NN
cord-288669-46tkedw7	169	34	prior	prior	RB
cord-288669-46tkedw7	169	35	to	to	IN
cord-288669-46tkedw7	169	36	genome	genome	NN
cord-288669-46tkedw7	169	37	replication	replication	NN
cord-288669-46tkedw7	169	38	.	.	.
cord-288669-46tkedw7	170	1	The	the	DT
cord-288669-46tkedw7	170	2	E	e	NN
cord-288669-46tkedw7	170	3	-	-	NN
cord-288669-46tkedw7	170	4	protein	protein	JJ
cord-288669-46tkedw7	170	5	-	-	HYPH
cord-288669-46tkedw7	170	6	negative	negative	JJ
cord-288669-46tkedw7	170	7	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	170	8	particles	particle	NNS
cord-288669-46tkedw7	170	9	were	be	VBD
cord-288669-46tkedw7	170	10	shown	show	VBN
cord-288669-46tkedw7	170	11	to	to	TO
cord-288669-46tkedw7	170	12	contain	contain	VB
cord-288669-46tkedw7	170	13	the	the	DT
cord-288669-46tkedw7	170	14	viral	viral	JJ
cord-288669-46tkedw7	170	15	genome	genome	NN
cord-288669-46tkedw7	170	16	and	and	CC
cord-288669-46tkedw7	170	17	able	able	JJ
cord-288669-46tkedw7	170	18	to	to	TO
cord-288669-46tkedw7	170	19	enter	enter	VB
cord-288669-46tkedw7	170	20	cells	cell	NNS
cord-288669-46tkedw7	170	21	but	but	CC
cord-288669-46tkedw7	170	22	unable	unable	JJ
cord-288669-46tkedw7	170	23	to	to	TO
cord-288669-46tkedw7	170	24	release	release	VB
cord-288669-46tkedw7	170	25	the	the	DT
cord-288669-46tkedw7	170	26	viral	viral	JJ
cord-288669-46tkedw7	170	27	RNA	rna	NN
cord-288669-46tkedw7	170	28	for	for	IN
cord-288669-46tkedw7	170	29	initiation	initiation	NN
cord-288669-46tkedw7	170	30	of	of	IN
cord-288669-46tkedw7	170	31	genome	genome	NN
cord-288669-46tkedw7	170	32	replication	replication	NN
cord-288669-46tkedw7	170	33	.	.	.
cord-288669-46tkedw7	171	1	This	this	DT
cord-288669-46tkedw7	171	2	suggests	suggest	VBZ
cord-288669-46tkedw7	171	3	that	that	IN
cord-288669-46tkedw7	171	4	PRRS	PRRS	NNP
cord-288669-46tkedw7	171	5	virions	virion	NNS
cord-288669-46tkedw7	171	6	may	may	MD
cord-288669-46tkedw7	171	7	contain	contain	VB
cord-288669-46tkedw7	171	8	ion	ion	NN
cord-288669-46tkedw7	171	9	channels	channel	NNS
cord-288669-46tkedw7	171	10	and	and	CC
cord-288669-46tkedw7	171	11	the	the	DT
cord-288669-46tkedw7	171	12	E	e	NN
cord-288669-46tkedw7	171	13	protein	protein	NN
cord-288669-46tkedw7	171	14	may	may	MD
cord-288669-46tkedw7	171	15	function	function	VB
cord-288669-46tkedw7	171	16	as	as	IN
cord-288669-46tkedw7	171	17	an	an	DT
cord-288669-46tkedw7	171	18	ion	ion	NN
cord-288669-46tkedw7	171	19	channel	channel	NN
cord-288669-46tkedw7	171	20	protein	protein	NN
cord-288669-46tkedw7	171	21	embedded	embed	VBN
cord-288669-46tkedw7	171	22	in	in	IN
cord-288669-46tkedw7	171	23	the	the	DT
cord-288669-46tkedw7	171	24	viral	viral	JJ
cord-288669-46tkedw7	171	25	envelope	envelope	NN
cord-288669-46tkedw7	171	26	.	.	.
cord-288669-46tkedw7	172	1	It	-PRON-	PRP
cord-288669-46tkedw7	172	2	is	be	VBZ
cord-288669-46tkedw7	172	3	postulated	postulate	VBN
cord-288669-46tkedw7	172	4	that	that	IN
cord-288669-46tkedw7	172	5	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	172	6	replication	replication	NN
cord-288669-46tkedw7	172	7	may	may	MD
cord-288669-46tkedw7	172	8	be	be	VB
cord-288669-46tkedw7	172	9	suppressed	suppress	VBN
cord-288669-46tkedw7	172	10	by	by	IN
cord-288669-46tkedw7	172	11	ion	ion	NN
cord-288669-46tkedw7	172	12	channel	channel	NN
cord-288669-46tkedw7	172	13	blocking	block	VBG
cord-288669-46tkedw7	172	14	agents	agent	NNS
cord-288669-46tkedw7	172	15	if	if	IN
cord-288669-46tkedw7	172	16	ion	ion	NN
cord-288669-46tkedw7	172	17	channel	channel	NN
cord-288669-46tkedw7	172	18	activity	activity	NN
cord-288669-46tkedw7	172	19	is	be	VBZ
cord-288669-46tkedw7	172	20	an	an	DT
cord-288669-46tkedw7	172	21	essential	essential	JJ
cord-288669-46tkedw7	172	22	requirement	requirement	NN
cord-288669-46tkedw7	172	23	for	for	IN
cord-288669-46tkedw7	172	24	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	172	25	infection	infection	NN
cord-288669-46tkedw7	172	26	.	.	.
cord-288669-46tkedw7	173	1	This	this	DT
cord-288669-46tkedw7	173	2	aspect	aspect	NN
cord-288669-46tkedw7	173	3	was	be	VBD
cord-288669-46tkedw7	173	4	investigated	investigate	VBN
cord-288669-46tkedw7	173	5	in	in	IN
cord-288669-46tkedw7	173	6	Marc-145	marc-145	DT
cord-288669-46tkedw7	173	7	cells	cell	NNS
cord-288669-46tkedw7	173	8	using	use	VBG
cord-288669-46tkedw7	173	9	amantadine	amantadine	NN
cord-288669-46tkedw7	173	10	and	and	CC
cord-288669-46tkedw7	173	11	verapamil	verapamil	NN
cord-288669-46tkedw7	173	12	.	.	.
cord-288669-46tkedw7	174	1	Amantadine	Amantadine	NNP
cord-288669-46tkedw7	174	2	is	be	VBZ
cord-288669-46tkedw7	174	3	known	know	VBN
cord-288669-46tkedw7	174	4	as	as	IN
cord-288669-46tkedw7	174	5	a	a	DT
cord-288669-46tkedw7	174	6	proton	proton	NN
cord-288669-46tkedw7	174	7	channel	channel	NN
cord-288669-46tkedw7	174	8	blocker	blocker	NN
cord-288669-46tkedw7	174	9	and	and	CC
cord-288669-46tkedw7	174	10	verapamil	verapamil	NN
cord-288669-46tkedw7	174	11	is	be	VBZ
cord-288669-46tkedw7	174	12	the	the	DT
cord-288669-46tkedw7	174	13	calcium	calcium	NN
cord-288669-46tkedw7	174	14	channel	channel	NN
cord-288669-46tkedw7	174	15	blocking	block	VBG
cord-288669-46tkedw7	174	16	agent	agent	NN
cord-288669-46tkedw7	174	17	.	.	.
cord-288669-46tkedw7	175	1	Because	because	IN
cord-288669-46tkedw7	175	2	it	-PRON-	PRP
cord-288669-46tkedw7	175	3	has	have	VBZ
cord-288669-46tkedw7	175	4	been	be	VBN
cord-288669-46tkedw7	175	5	reported	report	VBN
cord-288669-46tkedw7	175	6	that	that	IN
cord-288669-46tkedw7	175	7	an	an	DT
cord-288669-46tkedw7	175	8	acidic	acidic	JJ
cord-288669-46tkedw7	175	9	environment	environment	NN
cord-288669-46tkedw7	175	10	is	be	VBZ
cord-288669-46tkedw7	175	11	required	require	VBN
cord-288669-46tkedw7	175	12	for	for	IN
cord-288669-46tkedw7	175	13	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	175	14	infection	infection	NN
cord-288669-46tkedw7	175	15	(	(	-LRB-
cord-288669-46tkedw7	175	16	Kreutz	Kreutz	NNP
cord-288669-46tkedw7	175	17	and	and	CC
cord-288669-46tkedw7	175	18	Ackermann	Ackermann	NNP
cord-288669-46tkedw7	175	19	,	,	,
cord-288669-46tkedw7	175	20	1996	1996	CD
cord-288669-46tkedw7	175	21	)	)	-RRB-
cord-288669-46tkedw7	175	22	,	,	,
cord-288669-46tkedw7	175	23	basic	basic	JJ
cord-288669-46tkedw7	175	24	lysosomotropic	lysosomotropic	JJ
cord-288669-46tkedw7	175	25	agents	agent	NNS
cord-288669-46tkedw7	175	26	including	include	VBG
cord-288669-46tkedw7	175	27	ammonium	ammonium	NN
cord-288669-46tkedw7	175	28	chloride	chloride	NN
cord-288669-46tkedw7	175	29	and	and	CC
cord-288669-46tkedw7	175	30	chloroquine	chloroquine	NN
cord-288669-46tkedw7	175	31	were	be	VBD
cord-288669-46tkedw7	175	32	also	also	RB
cord-288669-46tkedw7	175	33	included	include	VBN
cord-288669-46tkedw7	175	34	in	in	IN
cord-288669-46tkedw7	175	35	the	the	DT
cord-288669-46tkedw7	175	36	study	study	NN
cord-288669-46tkedw7	175	37	to	to	TO
cord-288669-46tkedw7	175	38	examine	examine	VB
cord-288669-46tkedw7	175	39	their	-PRON-	PRP$
cord-288669-46tkedw7	175	40	inhibitory	inhibitory	JJ
cord-288669-46tkedw7	175	41	effects	effect	NNS
cord-288669-46tkedw7	175	42	on	on	IN
cord-288669-46tkedw7	175	43	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	175	44	replication	replication	NN
cord-288669-46tkedw7	175	45	.	.	.
cord-288669-46tkedw7	176	1	Strand	strand	NN
cord-288669-46tkedw7	176	2	-	-	HYPH
cord-288669-46tkedw7	176	3	specific	specific	JJ
cord-288669-46tkedw7	176	4	RT	RT	NNP
cord-288669-46tkedw7	176	5	-	-	HYPH
cord-288669-46tkedw7	176	6	PCR	PCR	NNP
cord-288669-46tkedw7	176	7	experiments	experiment	NNS
cord-288669-46tkedw7	176	8	were	be	VBD
cord-288669-46tkedw7	176	9	carried	carry	VBN
cord-288669-46tkedw7	176	10	out	out	RP
cord-288669-46tkedw7	176	11	and	and	CC
cord-288669-46tkedw7	176	12	demonstrated	demonstrate	VBD
cord-288669-46tkedw7	176	13	that	that	IN
cord-288669-46tkedw7	176	14	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	176	15	-	-	HYPH
cord-288669-46tkedw7	176	16	infected	infect	VBN
cord-288669-46tkedw7	176	17	Marc-145	marc-145	CD
cord-288669-46tkedw7	176	18	cells	cell	NNS
cord-288669-46tkedw7	176	19	produced	produce	VBN
cord-288669-46tkedw7	176	20	reduced	reduced	JJ
cord-288669-46tkedw7	176	21	levels	level	NNS
cord-288669-46tkedw7	176	22	of	of	IN
cord-288669-46tkedw7	176	23	positive	positive	JJ
cord-288669-46tkedw7	176	24	-	-	HYPH
cord-288669-46tkedw7	176	25	sense	sense	NN
cord-288669-46tkedw7	176	26	genomic	genomic	JJ
cord-288669-46tkedw7	176	27	RNA	rna	NN
cord-288669-46tkedw7	176	28	at	at	IN
cord-288669-46tkedw7	176	29	2	2	CD
cord-288669-46tkedw7	176	30	days	day	NNS
cord-288669-46tkedw7	176	31	post	post	NN
cord-288669-46tkedw7	176	32	-	-	NN
cord-288669-46tkedw7	176	33	infection	infection	NN
cord-288669-46tkedw7	176	34	in	in	IN
cord-288669-46tkedw7	176	35	the	the	DT
cord-288669-46tkedw7	176	36	presence	presence	NN
cord-288669-46tkedw7	176	37	of	of	IN
cord-288669-46tkedw7	176	38	the	the	DT
cord-288669-46tkedw7	176	39	drugs	drug	NNS
cord-288669-46tkedw7	176	40	chloroquine	chloroquine	NN
cord-288669-46tkedw7	176	41	,	,	,
cord-288669-46tkedw7	176	42	amantadine	amantadine	NN
cord-288669-46tkedw7	176	43	and	and	CC
cord-288669-46tkedw7	176	44	verapamil	verapamil	NN
cord-288669-46tkedw7	176	45	(	(	-LRB-
cord-288669-46tkedw7	176	46	Fig	Fig	NNP
cord-288669-46tkedw7	176	47	.	.	.
cord-288669-46tkedw7	176	48	5A	5a	CD
cord-288669-46tkedw7	176	49	,	,	,
cord-288669-46tkedw7	176	50	upper	upper	JJ
cord-288669-46tkedw7	176	51	panel	panel	NN
cord-288669-46tkedw7	176	52	)	)	-RRB-
cord-288669-46tkedw7	176	53	.	.	.
cord-288669-46tkedw7	177	1	A	a	DT
cord-288669-46tkedw7	177	2	relatively	relatively	RB
cord-288669-46tkedw7	177	3	moderate	moderate	JJ
cord-288669-46tkedw7	177	4	level	level	NN
cord-288669-46tkedw7	177	5	of	of	IN
cord-288669-46tkedw7	177	6	suppression	suppression	NN
cord-288669-46tkedw7	177	7	was	be	VBD
cord-288669-46tkedw7	177	8	observed	observe	VBN
cord-288669-46tkedw7	177	9	in	in	IN
cord-288669-46tkedw7	177	10	ammonium	ammonium	NN
cord-288669-46tkedw7	177	11	chloride	chloride	NN
cord-288669-46tkedw7	177	12	-	-	HYPH
cord-288669-46tkedw7	177	13	treated	treat	VBN
cord-288669-46tkedw7	177	14	cells	cell	NNS
cord-288669-46tkedw7	177	15	(	(	-LRB-
cord-288669-46tkedw7	177	16	lane	lane	NN
cord-288669-46tkedw7	177	17	4	4	CD
cord-288669-46tkedw7	177	18	)	)	-RRB-
cord-288669-46tkedw7	177	19	.	.	.
cord-288669-46tkedw7	178	1	Negative	negative	JJ
cord-288669-46tkedw7	178	2	-	-	HYPH
cord-288669-46tkedw7	178	3	sense	sense	NN
cord-288669-46tkedw7	178	4	genomic	genomic	JJ
cord-288669-46tkedw7	178	5	RNA	RNA	NNP
cord-288669-46tkedw7	178	6	was	be	VBD
cord-288669-46tkedw7	178	7	not	not	RB
cord-288669-46tkedw7	178	8	detectable	detectable	JJ
cord-288669-46tkedw7	178	9	in	in	IN
cord-288669-46tkedw7	178	10	cells	cell	NNS
cord-288669-46tkedw7	178	11	treated	treat	VBN
cord-288669-46tkedw7	178	12	with	with	IN
cord-288669-46tkedw7	178	13	any	any	DT
cord-288669-46tkedw7	178	14	of	of	IN
cord-288669-46tkedw7	178	15	the	the	DT
cord-288669-46tkedw7	178	16	four	four	CD
cord-288669-46tkedw7	178	17	drugs	drug	NNS
cord-288669-46tkedw7	178	18	until	until	IN
cord-288669-46tkedw7	178	19	2	2	CD
cord-288669-46tkedw7	178	20	days	day	NNS
cord-288669-46tkedw7	178	21	post	post	NN
cord-288669-46tkedw7	178	22	-	-	NN
cord-288669-46tkedw7	178	23	infection	infection	JJ
cord-288669-46tkedw7	178	24	(	(	-LRB-
cord-288669-46tkedw7	178	25	middle	middle	JJ
cord-288669-46tkedw7	178	26	and	and	CC
cord-288669-46tkedw7	178	27	lower	low	JJR
cord-288669-46tkedw7	178	28	panels	panel	NNS
cord-288669-46tkedw7	178	29	,	,	,
cord-288669-46tkedw7	178	30	lanes	lane	VBZ
cord-288669-46tkedw7	178	31	4	4	CD
cord-288669-46tkedw7	178	32	-	-	SYM
cord-288669-46tkedw7	178	33	7	7	CD
cord-288669-46tkedw7	178	34	)	)	-RRB-
cord-288669-46tkedw7	178	35	,	,	,
cord-288669-46tkedw7	178	36	showing	show	VBG
cord-288669-46tkedw7	178	37	that	that	IN
cord-288669-46tkedw7	178	38	the	the	DT
cord-288669-46tkedw7	178	39	initiation	initiation	NN
cord-288669-46tkedw7	178	40	of	of	IN
cord-288669-46tkedw7	178	41	viral	viral	JJ
cord-288669-46tkedw7	178	42	RNA	RNA	NNP
cord-288669-46tkedw7	178	43	synthesis	synthesis	NN
cord-288669-46tkedw7	178	44	was	be	VBD
cord-288669-46tkedw7	178	45	inhibited	inhibit	VBN
cord-288669-46tkedw7	178	46	by	by	IN
cord-288669-46tkedw7	178	47	the	the	DT
cord-288669-46tkedw7	178	48	ion	ion	NN
cord-288669-46tkedw7	178	49	channel	channel	NN
cord-288669-46tkedw7	178	50	blockers	blocker	NNS
cord-288669-46tkedw7	178	51	.	.	.
cord-288669-46tkedw7	179	1	Furthermore	furthermore	RB
cord-288669-46tkedw7	179	2	,	,	,
cord-288669-46tkedw7	179	3	the	the	DT
cord-288669-46tkedw7	179	4	number	number	NN
cord-288669-46tkedw7	179	5	of	of	IN
cord-288669-46tkedw7	179	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	179	7	-	-	HYPH
cord-288669-46tkedw7	179	8	infected	infect	VBN
cord-288669-46tkedw7	179	9	cells	cell	NNS
cord-288669-46tkedw7	179	10	determined	determine	VBN
cord-288669-46tkedw7	179	11	by	by	IN
cord-288669-46tkedw7	179	12	the	the	DT
cord-288669-46tkedw7	179	13	N	N	NNP
cord-288669-46tkedw7	179	14	protein	protein	NN
cord-288669-46tkedw7	179	15	staining	staining	NN
cord-288669-46tkedw7	179	16	was	be	VBD
cord-288669-46tkedw7	179	17	significantly	significantly	RB
cord-288669-46tkedw7	179	18	reduced	reduce	VBN
cord-288669-46tkedw7	179	19	by	by	IN
cord-288669-46tkedw7	179	20	individual	individual	JJ
cord-288669-46tkedw7	179	21	drugs	drug	NNS
cord-288669-46tkedw7	179	22	in	in	IN
cord-288669-46tkedw7	179	23	comparison	comparison	NN
cord-288669-46tkedw7	179	24	to	to	IN
cord-288669-46tkedw7	179	25	untreated	untreated	JJ
cord-288669-46tkedw7	179	26	cells	cell	NNS
cord-288669-46tkedw7	179	27	(	(	-LRB-
cord-288669-46tkedw7	179	28	Fig	Fig	NNP
cord-288669-46tkedw7	179	29	.	.	.
cord-288669-46tkedw7	179	30	5B	5b	CD
cord-288669-46tkedw7	179	31	,	,	,
cord-288669-46tkedw7	179	32	upper	upper	JJ
cord-288669-46tkedw7	179	33	panels	panel	NNS
cord-288669-46tkedw7	179	34	)	)	-RRB-
cord-288669-46tkedw7	179	35	.	.	.
cord-288669-46tkedw7	180	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	180	2	-	-	HYPH
cord-288669-46tkedw7	180	3	specific	specific	JJ
cord-288669-46tkedw7	180	4	CPE	CPE	NNP
cord-288669-46tkedw7	180	5	was	be	VBD
cord-288669-46tkedw7	180	6	visible	visible	JJ
cord-288669-46tkedw7	180	7	but	but	CC
cord-288669-46tkedw7	180	8	significantly	significantly	RB
cord-288669-46tkedw7	180	9	delayed	delay	VBN
cord-288669-46tkedw7	180	10	by	by	IN
cord-288669-46tkedw7	180	11	the	the	DT
cord-288669-46tkedw7	180	12	ion	ion	NN
cord-288669-46tkedw7	180	13	channel	channel	NN
cord-288669-46tkedw7	180	14	blockers	blocker	NNS
cord-288669-46tkedw7	180	15	,	,	,
cord-288669-46tkedw7	180	16	indicating	indicate	VBG
cord-288669-46tkedw7	180	17	the	the	DT
cord-288669-46tkedw7	180	18	negative	negative	JJ
cord-288669-46tkedw7	180	19	effects	effect	NNS
cord-288669-46tkedw7	180	20	on	on	IN
cord-288669-46tkedw7	180	21	virus	virus	NN
cord-288669-46tkedw7	180	22	production	production	NN
cord-288669-46tkedw7	180	23	(	(	-LRB-
cord-288669-46tkedw7	180	24	lower	low	JJR
cord-288669-46tkedw7	180	25	panels	panel	NNS
cord-288669-46tkedw7	180	26	)	)	-RRB-
cord-288669-46tkedw7	180	27	.	.	.
cord-288669-46tkedw7	181	1	The	the	DT
cord-288669-46tkedw7	181	2	yields	yield	NNS
cord-288669-46tkedw7	181	3	of	of	IN
cord-288669-46tkedw7	181	4	virus	virus	NN
cord-288669-46tkedw7	181	5	production	production	NN
cord-288669-46tkedw7	181	6	were	be	VBD
cord-288669-46tkedw7	181	7	determined	determine	VBN
cord-288669-46tkedw7	181	8	by	by	IN
cord-288669-46tkedw7	181	9	plaque	plaque	NN
cord-288669-46tkedw7	181	10	assays	assay	NNS
cord-288669-46tkedw7	181	11	from	from	IN
cord-288669-46tkedw7	181	12	the	the	DT
cord-288669-46tkedw7	181	13	supernatants	supernatant	NNS
cord-288669-46tkedw7	181	14	in	in	IN
cord-288669-46tkedw7	181	15	the	the	DT
cord-288669-46tkedw7	181	16	presence	presence	NN
cord-288669-46tkedw7	181	17	of	of	IN
cord-288669-46tkedw7	181	18	each	each	DT
cord-288669-46tkedw7	181	19	drug	drug	NN
cord-288669-46tkedw7	181	20	.	.	.
cord-288669-46tkedw7	182	1	The	the	DT
cord-288669-46tkedw7	182	2	virus	virus	NN
cord-288669-46tkedw7	182	3	titer	titer	NN
cord-288669-46tkedw7	182	4	in	in	IN
cord-288669-46tkedw7	182	5	untreated	untreated	JJ
cord-288669-46tkedw7	182	6	cells	cell	NNS
cord-288669-46tkedw7	182	7	reached	reach	VBD
cord-288669-46tkedw7	182	8	3	3	CD
cord-288669-46tkedw7	182	9	×	×	NN
cord-288669-46tkedw7	182	10	10	10	CD
cord-288669-46tkedw7	183	1	6	6	CD
cord-288669-46tkedw7	183	2	PFU	pfu	NN
cord-288669-46tkedw7	183	3	/	/	SYM
cord-288669-46tkedw7	183	4	ml	ml	NN
cord-288669-46tkedw7	183	5	by	by	IN
cord-288669-46tkedw7	183	6	3	3	CD
cord-288669-46tkedw7	183	7	days	day	NNS
cord-288669-46tkedw7	183	8	post	post	NN
cord-288669-46tkedw7	183	9	-	-	NN
cord-288669-46tkedw7	183	10	infection	infection	JJ
cord-288669-46tkedw7	184	1	(	(	-LRB-
cord-288669-46tkedw7	184	2	Fig	Fig	NNP
cord-288669-46tkedw7	184	3	.	.	.
cord-288669-46tkedw7	184	4	5C	5c	CD
cord-288669-46tkedw7	184	5	)	)	-RRB-
cord-288669-46tkedw7	184	6	.	.	.
cord-288669-46tkedw7	185	1	In	in	IN
cord-288669-46tkedw7	185	2	contrast	contrast	NN
cord-288669-46tkedw7	185	3	,	,	,
cord-288669-46tkedw7	185	4	Fig	Fig	NNP
cord-288669-46tkedw7	185	5	.	.	NNP
cord-288669-46tkedw7	185	6	4	4	CD
cord-288669-46tkedw7	185	7	.	.	.
cord-288669-46tkedw7	186	1	The	the	DT
cord-288669-46tkedw7	186	2	E	e	NN
cord-288669-46tkedw7	186	3	protein	protein	NN
cord-288669-46tkedw7	186	4	is	be	VBZ
cord-288669-46tkedw7	186	5	essential	essential	JJ
cord-288669-46tkedw7	186	6	for	for	IN
cord-288669-46tkedw7	186	7	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	186	8	replication	replication	NN
cord-288669-46tkedw7	186	9	.	.	.
cord-288669-46tkedw7	187	1	(	(	-LRB-
cord-288669-46tkedw7	187	2	A	a	NN
cord-288669-46tkedw7	187	3	)	)	-RRB-
cord-288669-46tkedw7	187	4	Immunofluorescence	Immunofluorescence	NNP
cord-288669-46tkedw7	187	5	of	of	IN
cord-288669-46tkedw7	187	6	N	n	CD
cord-288669-46tkedw7	187	7	protein	protein	NN
cord-288669-46tkedw7	187	8	in	in	IN
cord-288669-46tkedw7	187	9	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	187	10	inoculated	inoculate	VBN
cord-288669-46tkedw7	187	11	cells	cell	NNS
cord-288669-46tkedw7	187	12	.	.	.
cord-288669-46tkedw7	188	1	The	the	DT
cord-288669-46tkedw7	188	2	'	'	``
cord-288669-46tkedw7	188	3	passage-1	passage-1	NNP
cord-288669-46tkedw7	188	4	'	'	''
cord-288669-46tkedw7	188	5	virus	virus	NN
cord-288669-46tkedw7	188	6	was	be	VBD
cord-288669-46tkedw7	188	7	prepared	prepare	VBN
cord-288669-46tkedw7	188	8	as	as	IN
cord-288669-46tkedw7	188	9	culture	culture	NN
cord-288669-46tkedw7	188	10	supernatant	supernatant	VBN
cord-288669-46tkedw7	188	11	harvested	harvest	VBN
cord-288669-46tkedw7	188	12	from	from	IN
cord-288669-46tkedw7	188	13	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	188	14	cells	cell	NNS
cord-288669-46tkedw7	188	15	transfected	transfecte	VBN
cord-288669-46tkedw7	188	16	with	with	IN
cord-288669-46tkedw7	188	17	P129-WT	p129-wt	CD
cord-288669-46tkedw7	188	18	or	or	CC
cord-288669-46tkedw7	188	19	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	188	20	clone	clone	NN
cord-288669-46tkedw7	188	21	.	.	.
cord-288669-46tkedw7	189	1	Marc-145	marc-145	NN
cord-288669-46tkedw7	189	2	cells	cell	NNS
cord-288669-46tkedw7	189	3	were	be	VBD
cord-288669-46tkedw7	189	4	inoculated	inoculate	VBN
cord-288669-46tkedw7	189	5	with	with	IN
cord-288669-46tkedw7	189	6	'	'	``
cord-288669-46tkedw7	189	7	passage-1	passage-1	NNP
cord-288669-46tkedw7	189	8	'	'	''
cord-288669-46tkedw7	189	9	and	and	CC
cord-288669-46tkedw7	189	10	fixed	fix	VBN
cord-288669-46tkedw7	189	11	at	at	IN
cord-288669-46tkedw7	189	12	the	the	DT
cord-288669-46tkedw7	189	13	indicated	indicate	VBN
cord-288669-46tkedw7	189	14	times	times	NNP
cord-288669-46tkedw7	189	15	post	post	JJ
cord-288669-46tkedw7	189	16	-	-	NN
cord-288669-46tkedw7	189	17	inoculation	inoculation	NN
cord-288669-46tkedw7	189	18	,	,	,
cord-288669-46tkedw7	189	19	followed	follow	VBN
cord-288669-46tkedw7	189	20	by	by	IN
cord-288669-46tkedw7	189	21	staining	stain	VBG
cord-288669-46tkedw7	189	22	with	with	IN
cord-288669-46tkedw7	189	23	N	n	CD
cord-288669-46tkedw7	189	24	-	-	HYPH
cord-288669-46tkedw7	189	25	specific	specific	JJ
cord-288669-46tkedw7	189	26	MAb	MAb	NNS
cord-288669-46tkedw7	189	27	.	.	.
cord-288669-46tkedw7	190	1	(	(	-LRB-
cord-288669-46tkedw7	190	2	B	b	NN
cord-288669-46tkedw7	190	3	)	)	-RRB-
cord-288669-46tkedw7	190	4	Detection	detection	NN
cord-288669-46tkedw7	190	5	of	of	IN
cord-288669-46tkedw7	190	6	viral	viral	JJ
cord-288669-46tkedw7	190	7	RNA	RNA	NNP
cord-288669-46tkedw7	190	8	in	in	IN
cord-288669-46tkedw7	190	9	culture	culture	NN
cord-288669-46tkedw7	190	10	supernatants	supernatant	NNS
cord-288669-46tkedw7	190	11	and	and	CC
cord-288669-46tkedw7	190	12	in	in	IN
cord-288669-46tkedw7	190	13	Marc-145	marc-145	DT
cord-288669-46tkedw7	190	14	cells	cell	NNS
cord-288669-46tkedw7	190	15	inoculated	inoculate	VBN
cord-288669-46tkedw7	190	16	with	with	IN
cord-288669-46tkedw7	190	17	'	'	``
cord-288669-46tkedw7	190	18	passage-1	passage-1	NNP
cord-288669-46tkedw7	190	19	'	'	''
cord-288669-46tkedw7	190	20	or	or	CC
cord-288669-46tkedw7	190	21	'	'	``
cord-288669-46tkedw7	190	22	passage-2	passage-2	NNP
cord-288669-46tkedw7	190	23	'	'	''
cord-288669-46tkedw7	190	24	virus	virus	NN
cord-288669-46tkedw7	190	25	.	.	.
cord-288669-46tkedw7	191	1	Total	Total	NNP
cord-288669-46tkedw7	191	2	RNA	RNA	NNP
cord-288669-46tkedw7	191	3	was	be	VBD
cord-288669-46tkedw7	191	4	extracted	extract	VBN
cord-288669-46tkedw7	191	5	and	and	CC
cord-288669-46tkedw7	191	6	treated	treat	VBN
cord-288669-46tkedw7	191	7	with	with	IN
cord-288669-46tkedw7	191	8	DNase	DNase	NNP
cord-288669-46tkedw7	192	1	I	-PRON-	PRP
cord-288669-46tkedw7	192	2	followed	follow	VBD
cord-288669-46tkedw7	192	3	by	by	IN
cord-288669-46tkedw7	192	4	RT	RT	NNP
cord-288669-46tkedw7	192	5	-	-	HYPH
cord-288669-46tkedw7	192	6	PCR	PCR	NNP
cord-288669-46tkedw7	192	7	for	for	IN
cord-288669-46tkedw7	192	8	E	e	NN
cord-288669-46tkedw7	192	9	gene	gene	NN
cord-288669-46tkedw7	192	10	.	.	.
cord-288669-46tkedw7	193	1	(	(	-LRB-
cord-288669-46tkedw7	193	2	C	C	NNP
cord-288669-46tkedw7	193	3	)	)	-RRB-
cord-288669-46tkedw7	193	4	Strand	strand	NN
cord-288669-46tkedw7	193	5	-	-	HYPH
cord-288669-46tkedw7	193	6	specific	specific	JJ
cord-288669-46tkedw7	193	7	detection	detection	NN
cord-288669-46tkedw7	193	8	of	of	IN
cord-288669-46tkedw7	193	9	viral	viral	JJ
cord-288669-46tkedw7	193	10	RNA	RNA	NNP
cord-288669-46tkedw7	193	11	in	in	IN
cord-288669-46tkedw7	193	12	cells	cell	NNS
cord-288669-46tkedw7	193	13	by	by	IN
cord-288669-46tkedw7	193	14	RT	RT	NNP
cord-288669-46tkedw7	193	15	-	-	HYPH
cord-288669-46tkedw7	193	16	PCR	PCR	NNP
cord-288669-46tkedw7	193	17	.	.	.
cord-288669-46tkedw7	194	1	Marc-145	marc-145	NN
cord-288669-46tkedw7	194	2	cells	cell	NNS
cord-288669-46tkedw7	194	3	were	be	VBD
cord-288669-46tkedw7	194	4	inoculated	inoculate	VBN
cord-288669-46tkedw7	194	5	with	with	IN
cord-288669-46tkedw7	194	6	'	'	``
cord-288669-46tkedw7	194	7	passage-1	passage-1	NNP
cord-288669-46tkedw7	194	8	'	'	POS
cord-288669-46tkedw7	194	9	P129-WT	p129-wt	CD
cord-288669-46tkedw7	194	10	or	or	CC
cord-288669-46tkedw7	194	11	'	'	``
cord-288669-46tkedw7	194	12	passage-1	passage-1	NNP
cord-288669-46tkedw7	194	13	'	'	POS
cord-288669-46tkedw7	194	14	P129-ΔE	p129-δe	CD
cord-288669-46tkedw7	194	15	,	,	,
cord-288669-46tkedw7	194	16	and	and	CC
cord-288669-46tkedw7	194	17	total	total	JJ
cord-288669-46tkedw7	194	18	cellular	cellular	JJ
cord-288669-46tkedw7	194	19	RNA	RNA	NNP
cord-288669-46tkedw7	194	20	was	be	VBD
cord-288669-46tkedw7	194	21	extracted	extract	VBN
cord-288669-46tkedw7	194	22	at	at	IN
cord-288669-46tkedw7	194	23	2	2	CD
cord-288669-46tkedw7	194	24	days	day	NNS
cord-288669-46tkedw7	194	25	post	post	NN
cord-288669-46tkedw7	194	26	-	-	NN
cord-288669-46tkedw7	194	27	inoculation	inoculation	NN
cord-288669-46tkedw7	194	28	.	.	.
cord-288669-46tkedw7	195	1	The	the	DT
cord-288669-46tkedw7	195	2	RNA	RNA	NNP
cord-288669-46tkedw7	195	3	was	be	VBD
cord-288669-46tkedw7	195	4	treated	treat	VBN
cord-288669-46tkedw7	195	5	by	by	IN
cord-288669-46tkedw7	196	1	DNase	DNase	NNP
cord-288669-46tkedw7	196	2	I	i	NN
cord-288669-46tkedw7	196	3	and	and	CC
cord-288669-46tkedw7	196	4	RT	RT	NNP
cord-288669-46tkedw7	196	5	-	-	HYPH
cord-288669-46tkedw7	196	6	PCR	PCR	NNP
cord-288669-46tkedw7	196	7	was	be	VBD
cord-288669-46tkedw7	196	8	conducted	conduct	VBN
cord-288669-46tkedw7	196	9	to	to	TO
cord-288669-46tkedw7	196	10	amplify	amplify	VB
cord-288669-46tkedw7	196	11	the	the	DT
cord-288669-46tkedw7	196	12	region	region	NN
cord-288669-46tkedw7	196	13	as	as	IN
cord-288669-46tkedw7	196	14	illustrated	illustrate	VBN
cord-288669-46tkedw7	196	15	in	in	IN
cord-288669-46tkedw7	196	16	the	the	DT
cord-288669-46tkedw7	196	17	figure	figure	NN
cord-288669-46tkedw7	196	18	.	.	.
cord-288669-46tkedw7	197	1	Numbers	number	NNS
cord-288669-46tkedw7	197	2	in	in	IN
cord-288669-46tkedw7	197	3	parenthesis	parenthesis	NN
cord-288669-46tkedw7	197	4	indicate	indicate	VBP
cord-288669-46tkedw7	197	5	the	the	DT
cord-288669-46tkedw7	197	6	5′	5′	CD
cord-288669-46tkedw7	197	7	most	most	RBS
cord-288669-46tkedw7	197	8	nucleotide	nucleotide	JJ
cord-288669-46tkedw7	197	9	position	position	NN
cord-288669-46tkedw7	197	10	in	in	IN
cord-288669-46tkedw7	197	11	each	each	DT
cord-288669-46tkedw7	197	12	primer	primer	NN
cord-288669-46tkedw7	197	13	with	with	IN
cord-288669-46tkedw7	197	14	respect	respect	NN
cord-288669-46tkedw7	197	15	to	to	IN
cord-288669-46tkedw7	197	16	the	the	DT
cord-288669-46tkedw7	197	17	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	197	18	genome	genome	NN
cord-288669-46tkedw7	197	19	.	.	.
cord-288669-46tkedw7	198	1	Expected	expect	VBN
cord-288669-46tkedw7	198	2	sizes	size	NNS
cord-288669-46tkedw7	198	3	of	of	IN
cord-288669-46tkedw7	198	4	amplified	amplified	JJ
cord-288669-46tkedw7	198	5	products	product	NNS
cord-288669-46tkedw7	198	6	are	be	VBP
cord-288669-46tkedw7	198	7	indicated	indicate	VBN
cord-288669-46tkedw7	198	8	on	on	IN
cord-288669-46tkedw7	198	9	the	the	DT
cord-288669-46tkedw7	198	10	right	right	NN
cord-288669-46tkedw7	198	11	of	of	IN
cord-288669-46tkedw7	198	12	the	the	DT
cord-288669-46tkedw7	198	13	gel	gel	NN
cord-288669-46tkedw7	198	14	.	.	.
cord-288669-46tkedw7	199	1	there	there	EX
cord-288669-46tkedw7	199	2	was	be	VBD
cord-288669-46tkedw7	199	3	no	no	DT
cord-288669-46tkedw7	199	4	detectable	detectable	JJ
cord-288669-46tkedw7	199	5	virus	virus	NN
cord-288669-46tkedw7	199	6	production	production	NN
cord-288669-46tkedw7	199	7	at	at	IN
cord-288669-46tkedw7	199	8	1	1	CD
cord-288669-46tkedw7	199	9	day	day	NN
cord-288669-46tkedw7	199	10	post	post	NN
cord-288669-46tkedw7	199	11	-	-	NN
cord-288669-46tkedw7	199	12	infection	infection	NN
cord-288669-46tkedw7	199	13	in	in	IN
cord-288669-46tkedw7	199	14	the	the	DT
cord-288669-46tkedw7	199	15	presence	presence	NN
cord-288669-46tkedw7	199	16	of	of	IN
cord-288669-46tkedw7	199	17	amantadine	amantadine	NNP
cord-288669-46tkedw7	199	18	or	or	CC
cord-288669-46tkedw7	199	19	verapamil	verapamil	NN
cord-288669-46tkedw7	199	20	,	,	,
cord-288669-46tkedw7	199	21	and	and	CC
cord-288669-46tkedw7	199	22	the	the	DT
cord-288669-46tkedw7	199	23	titers	titer	NNS
cord-288669-46tkedw7	199	24	of	of	IN
cord-288669-46tkedw7	199	25	virus	virus	NN
cord-288669-46tkedw7	199	26	production	production	NN
cord-288669-46tkedw7	199	27	in	in	IN
cord-288669-46tkedw7	199	28	the	the	DT
cord-288669-46tkedw7	199	29	presence	presence	NN
cord-288669-46tkedw7	199	30	of	of	IN
cord-288669-46tkedw7	199	31	these	these	DT
cord-288669-46tkedw7	199	32	drugs	drug	NNS
cord-288669-46tkedw7	199	33	reached	reach	VBD
cord-288669-46tkedw7	199	34	only	only	RB
cord-288669-46tkedw7	199	35	to	to	IN
cord-288669-46tkedw7	199	36	a	a	DT
cord-288669-46tkedw7	199	37	maximum	maximum	NN
cord-288669-46tkedw7	199	38	of	of	IN
cord-288669-46tkedw7	199	39	5	5	CD
cord-288669-46tkedw7	199	40	to	to	TO
cord-288669-46tkedw7	199	41	8	8	CD
cord-288669-46tkedw7	199	42	×	×	NN
cord-288669-46tkedw7	199	43	10	10	CD
cord-288669-46tkedw7	199	44	1	1	CD
cord-288669-46tkedw7	199	45	PFU	pfu	NN
cord-288669-46tkedw7	199	46	/	/	SYM
cord-288669-46tkedw7	199	47	ml	ml	NN
cord-288669-46tkedw7	199	48	by	by	IN
cord-288669-46tkedw7	199	49	3	3	CD
cord-288669-46tkedw7	199	50	days	day	NNS
cord-288669-46tkedw7	199	51	post	post	NN
cord-288669-46tkedw7	199	52	-	-	NN
cord-288669-46tkedw7	199	53	infection	infection	JJ
cord-288669-46tkedw7	200	1	(	(	-LRB-
cord-288669-46tkedw7	200	2	Fig	Fig	NNP
cord-288669-46tkedw7	200	3	.	.	.
cord-288669-46tkedw7	200	4	 	 	_SP
cord-288669-46tkedw7	200	5	5C	5c	CD
cord-288669-46tkedw7	200	6	)	)	-RRB-
cord-288669-46tkedw7	200	7	.	.	.
cord-288669-46tkedw7	201	1	These	these	DT
cord-288669-46tkedw7	201	2	results	result	NNS
cord-288669-46tkedw7	201	3	showed	show	VBD
cord-288669-46tkedw7	201	4	that	that	IN
cord-288669-46tkedw7	201	5	the	the	DT
cord-288669-46tkedw7	201	6	treatment	treatment	NN
cord-288669-46tkedw7	201	7	of	of	IN
cord-288669-46tkedw7	201	8	infected	infected	JJ
cord-288669-46tkedw7	201	9	cells	cell	NNS
cord-288669-46tkedw7	201	10	with	with	IN
cord-288669-46tkedw7	201	11	ion	ion	NN
cord-288669-46tkedw7	201	12	channel	channel	NN
cord-288669-46tkedw7	201	13	blockers	blocker	NNS
cord-288669-46tkedw7	201	14	greatly	greatly	RB
cord-288669-46tkedw7	201	15	reduced	reduce	VBD
cord-288669-46tkedw7	201	16	the	the	DT
cord-288669-46tkedw7	201	17	growth	growth	NN
cord-288669-46tkedw7	201	18	rate	rate	NN
cord-288669-46tkedw7	201	19	of	of	IN
cord-288669-46tkedw7	201	20	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	201	21	.	.	.
cord-288669-46tkedw7	202	1	However	however	RB
cord-288669-46tkedw7	202	2	,	,	,
cord-288669-46tkedw7	202	3	when	when	WRB
cord-288669-46tkedw7	202	4	Marc-145	marc-145	NN
cord-288669-46tkedw7	202	5	cells	cell	NNS
cord-288669-46tkedw7	202	6	were	be	VBD
cord-288669-46tkedw7	202	7	treated	treat	VBN
cord-288669-46tkedw7	202	8	with	with	IN
cord-288669-46tkedw7	202	9	the	the	DT
cord-288669-46tkedw7	202	10	drugs	drug	NNS
cord-288669-46tkedw7	202	11	at	at	IN
cord-288669-46tkedw7	202	12	30	30	CD
cord-288669-46tkedw7	202	13	min	min	NN
cord-288669-46tkedw7	202	14	post	post	NN
cord-288669-46tkedw7	202	15	-	-	NN
cord-288669-46tkedw7	202	16	infection	infection	NN
cord-288669-46tkedw7	202	17	,	,	,
cord-288669-46tkedw7	202	18	no	no	DT
cord-288669-46tkedw7	202	19	significant	significant	JJ
cord-288669-46tkedw7	202	20	inhibitory	inhibitory	JJ
cord-288669-46tkedw7	202	21	effect	effect	NN
cord-288669-46tkedw7	202	22	of	of	IN
cord-288669-46tkedw7	202	23	virus	virus	NN
cord-288669-46tkedw7	202	24	production	production	NN
cord-288669-46tkedw7	202	25	was	be	VBD
cord-288669-46tkedw7	202	26	observed	observe	VBN
cord-288669-46tkedw7	202	27	(	(	-LRB-
cord-288669-46tkedw7	202	28	data	datum	NNS
cord-288669-46tkedw7	202	29	not	not	RB
cord-288669-46tkedw7	202	30	shown	show	VBN
cord-288669-46tkedw7	202	31	)	)	-RRB-
cord-288669-46tkedw7	202	32	.	.	.
cord-288669-46tkedw7	203	1	Therefore	therefore	RB
cord-288669-46tkedw7	203	2	,	,	,
cord-288669-46tkedw7	203	3	it	-PRON-	PRP
cord-288669-46tkedw7	203	4	seems	seem	VBZ
cord-288669-46tkedw7	203	5	that	that	IN
cord-288669-46tkedw7	203	6	the	the	DT
cord-288669-46tkedw7	203	7	ion	ion	NN
cord-288669-46tkedw7	203	8	channel	channel	NN
cord-288669-46tkedw7	203	9	blockers	blocker	NNS
cord-288669-46tkedw7	203	10	effectively	effectively	RB
cord-288669-46tkedw7	203	11	interfered	interfere	VBD
cord-288669-46tkedw7	203	12	with	with	IN
cord-288669-46tkedw7	203	13	virus	virus	NN
cord-288669-46tkedw7	203	14	uncoating	uncoating	NN
cord-288669-46tkedw7	203	15	,	,	,
cord-288669-46tkedw7	203	16	a	a	DT
cord-288669-46tkedw7	203	17	step	step	NN
cord-288669-46tkedw7	203	18	preceding	precede	VBG
cord-288669-46tkedw7	203	19	genome	genome	NN
cord-288669-46tkedw7	203	20	replication	replication	NN
cord-288669-46tkedw7	203	21	and	and	CC
cord-288669-46tkedw7	203	22	consequently	consequently	RB
cord-288669-46tkedw7	203	23	affected	affect	VBD
cord-288669-46tkedw7	203	24	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	203	25	production	production	NN
cord-288669-46tkedw7	203	26	.	.	.
cord-288669-46tkedw7	204	1	It	-PRON-	PRP
cord-288669-46tkedw7	204	2	is	be	VBZ
cord-288669-46tkedw7	204	3	interesting	interesting	JJ
cord-288669-46tkedw7	204	4	to	to	TO
cord-288669-46tkedw7	204	5	note	note	VB
cord-288669-46tkedw7	204	6	that	that	IN
cord-288669-46tkedw7	204	7	the	the	DT
cord-288669-46tkedw7	204	8	inhibitory	inhibitory	JJ
cord-288669-46tkedw7	204	9	effect	effect	NN
cord-288669-46tkedw7	204	10	by	by	IN
cord-288669-46tkedw7	204	11	amantadine	amantadine	NNP
cord-288669-46tkedw7	204	12	are	be	VBP
cord-288669-46tkedw7	204	13	due	due	JJ
cord-288669-46tkedw7	204	14	to	to	IN
cord-288669-46tkedw7	204	15	the	the	DT
cord-288669-46tkedw7	204	16	blocking	blocking	NN
cord-288669-46tkedw7	204	17	of	of	IN
cord-288669-46tkedw7	204	18	pores	pore	NNS
cord-288669-46tkedw7	204	19	that	that	WDT
cord-288669-46tkedw7	204	20	are	be	VBP
cord-288669-46tkedw7	204	21	necessary	necessary	JJ
cord-288669-46tkedw7	204	22	for	for	IN
cord-288669-46tkedw7	204	23	post	post	JJ
cord-288669-46tkedw7	204	24	-	-	NN
cord-288669-46tkedw7	204	25	internalization	internalization	NN
cord-288669-46tkedw7	204	26	during	during	IN
cord-288669-46tkedw7	204	27	uncoating	uncoating	NN
cord-288669-46tkedw7	204	28	of	of	IN
cord-288669-46tkedw7	204	29	the	the	DT
cord-288669-46tkedw7	204	30	virus	virus	NN
cord-288669-46tkedw7	205	1	(	(	-LRB-
cord-288669-46tkedw7	205	2	Wang	Wang	NNP
cord-288669-46tkedw7	205	3	et	et	NNP
cord-288669-46tkedw7	205	4	al	al	NNP
cord-288669-46tkedw7	205	5	.	.	NNP
cord-288669-46tkedw7	205	6	,	,	,
cord-288669-46tkedw7	205	7	1993	1993	CD
cord-288669-46tkedw7	205	8	)	)	-RRB-
cord-288669-46tkedw7	205	9	.	.	.
cord-288669-46tkedw7	206	1	Because	because	IN
cord-288669-46tkedw7	206	2	our	-PRON-	PRP$
cord-288669-46tkedw7	206	3	data	datum	NNS
cord-288669-46tkedw7	206	4	suggest	suggest	VBP
cord-288669-46tkedw7	206	5	that	that	IN
cord-288669-46tkedw7	206	6	ion	ion	NN
cord-288669-46tkedw7	206	7	channel	channel	NN
cord-288669-46tkedw7	206	8	activity	activity	NN
cord-288669-46tkedw7	206	9	may	may	MD
cord-288669-46tkedw7	206	10	be	be	VB
cord-288669-46tkedw7	206	11	involved	involve	VBN
cord-288669-46tkedw7	206	12	during	during	IN
cord-288669-46tkedw7	206	13	uncoating	uncoate	VBG
cord-288669-46tkedw7	206	14	and	and	CC
cord-288669-46tkedw7	206	15	that	that	IN
cord-288669-46tkedw7	206	16	the	the	DT
cord-288669-46tkedw7	206	17	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	206	18	E	E	NNP
cord-288669-46tkedw7	206	19	protein	protein	NN
cord-288669-46tkedw7	206	20	is	be	VBZ
cord-288669-46tkedw7	206	21	likely	likely	RB
cord-288669-46tkedw7	206	22	responsible	responsible	JJ
cord-288669-46tkedw7	206	23	for	for	IN
cord-288669-46tkedw7	206	24	this	this	DT
cord-288669-46tkedw7	206	25	event	event	NN
cord-288669-46tkedw7	206	26	,	,	,
cord-288669-46tkedw7	206	27	the	the	DT
cord-288669-46tkedw7	206	28	E	e	NN
cord-288669-46tkedw7	206	29	protein	protein	NN
cord-288669-46tkedw7	206	30	was	be	VBD
cord-288669-46tkedw7	206	31	assumed	assume	VBN
cord-288669-46tkedw7	206	32	to	to	TO
cord-288669-46tkedw7	206	33	function	function	VB
cord-288669-46tkedw7	206	34	as	as	IN
cord-288669-46tkedw7	206	35	a	a	DT
cord-288669-46tkedw7	206	36	viroporin	viroporin	NN
cord-288669-46tkedw7	206	37	.	.	.
cord-288669-46tkedw7	207	1	We	-PRON-	PRP
cord-288669-46tkedw7	207	2	examined	examine	VBD
cord-288669-46tkedw7	207	3	whether	whether	IN
cord-288669-46tkedw7	207	4	the	the	DT
cord-288669-46tkedw7	207	5	E	e	NN
cord-288669-46tkedw7	207	6	protein	protein	NN
cord-288669-46tkedw7	207	7	possessed	possess	VBD
cord-288669-46tkedw7	207	8	general	general	JJ
cord-288669-46tkedw7	207	9	features	feature	NNS
cord-288669-46tkedw7	207	10	commonly	commonly	RB
cord-288669-46tkedw7	207	11	shared	share	VBN
cord-288669-46tkedw7	207	12	by	by	IN
cord-288669-46tkedw7	207	13	other	other	JJ
cord-288669-46tkedw7	207	14	viroporins	viroporin	NNS
cord-288669-46tkedw7	207	15	(	(	-LRB-
cord-288669-46tkedw7	207	16	ion	ion	NN
cord-288669-46tkedw7	207	17	channel	channel	NN
cord-288669-46tkedw7	207	18	proteins	protein	NNS
cord-288669-46tkedw7	207	19	)	)	-RRB-
cord-288669-46tkedw7	207	20	.	.	.
cord-288669-46tkedw7	208	1	Viroporins	viroporin	NNS
cord-288669-46tkedw7	208	2	generally	generally	RB
cord-288669-46tkedw7	208	3	contain	contain	VBP
cord-288669-46tkedw7	208	4	the	the	DT
cord-288669-46tkedw7	208	5	properties	property	NNS
cord-288669-46tkedw7	208	6	of	of	IN
cord-288669-46tkedw7	208	7	membrane	membrane	NN
cord-288669-46tkedw7	208	8	permeability	permeability	NN
cord-288669-46tkedw7	208	9	alteration	alteration	NN
cord-288669-46tkedw7	208	10	and	and	CC
cord-288669-46tkedw7	208	11	oligomerization	oligomerization	NN
cord-288669-46tkedw7	208	12	(	(	-LRB-
cord-288669-46tkedw7	208	13	Liao	Liao	NNP
cord-288669-46tkedw7	208	14	et	et	FW
cord-288669-46tkedw7	208	15	al	al	NNP
cord-288669-46tkedw7	208	16	.	.	NNP
cord-288669-46tkedw7	208	17	,	,	,
cord-288669-46tkedw7	208	18	2004	2004	CD
cord-288669-46tkedw7	208	19	;	;	:
cord-288669-46tkedw7	208	20	Maldarelli	Maldarelli	NNP
cord-288669-46tkedw7	208	21	et	et	NNP
cord-288669-46tkedw7	208	22	al	al	NNP
cord-288669-46tkedw7	208	23	.	.	NNP
cord-288669-46tkedw7	208	24	,	,	,
cord-288669-46tkedw7	208	25	1993	1993	CD
cord-288669-46tkedw7	208	26	;	;	:
cord-288669-46tkedw7	208	27	Paterson	Paterson	NNP
cord-288669-46tkedw7	208	28	et	et	NNP
cord-288669-46tkedw7	208	29	al	al	NNP
cord-288669-46tkedw7	208	30	.	.	NNP
cord-288669-46tkedw7	208	31	,	,	,
cord-288669-46tkedw7	208	32	2003	2003	CD
cord-288669-46tkedw7	208	33	;	;	:
cord-288669-46tkedw7	208	34	Sakaguchi	Sakaguchi	NNP
cord-288669-46tkedw7	208	35	et	et	NNP
cord-288669-46tkedw7	208	36	al	al	NNP
cord-288669-46tkedw7	208	37	.	.	NNP
cord-288669-46tkedw7	208	38	,	,	,
cord-288669-46tkedw7	208	39	1997	1997	CD
cord-288669-46tkedw7	208	40	;	;	:
cord-288669-46tkedw7	208	41	Torres	Torres	NNP
cord-288669-46tkedw7	208	42	et	et	NNP
cord-288669-46tkedw7	208	43	al	al	NNP
cord-288669-46tkedw7	208	44	.	.	NNP
cord-288669-46tkedw7	208	45	,	,	,
cord-288669-46tkedw7	208	46	2005	2005	CD
cord-288669-46tkedw7	208	47	)	)	-RRB-
cord-288669-46tkedw7	208	48	,	,	,
cord-288669-46tkedw7	208	49	and	and	CC
cord-288669-46tkedw7	208	50	so	so	RB
cord-288669-46tkedw7	208	51	these	these	DT
cord-288669-46tkedw7	208	52	two	two	CD
cord-288669-46tkedw7	208	53	common	common	JJ
cord-288669-46tkedw7	208	54	properties	property	NNS
cord-288669-46tkedw7	208	55	were	be	VBD
cord-288669-46tkedw7	208	56	examined	examine	VBN
cord-288669-46tkedw7	208	57	for	for	IN
cord-288669-46tkedw7	208	58	the	the	DT
cord-288669-46tkedw7	208	59	E	e	NN
cord-288669-46tkedw7	208	60	protein	protein	NN
cord-288669-46tkedw7	208	61	.	.	.
cord-288669-46tkedw7	209	1	The	the	DT
cord-288669-46tkedw7	209	2	effect	effect	NN
cord-288669-46tkedw7	209	3	of	of	IN
cord-288669-46tkedw7	209	4	E	e	NN
cord-288669-46tkedw7	209	5	expression	expression	NN
cord-288669-46tkedw7	209	6	on	on	IN
cord-288669-46tkedw7	209	7	bacterial	bacterial	JJ
cord-288669-46tkedw7	209	8	growth	growth	NN
cord-288669-46tkedw7	209	9	was	be	VBD
cord-288669-46tkedw7	209	10	first	first	RB
cord-288669-46tkedw7	209	11	examined	examine	VBN
cord-288669-46tkedw7	209	12	in	in	IN
cord-288669-46tkedw7	209	13	an	an	DT
cord-288669-46tkedw7	209	14	inducible	inducible	JJ
cord-288669-46tkedw7	209	15	protein	protein	NN
cord-288669-46tkedw7	209	16	expression	expression	NN
cord-288669-46tkedw7	209	17	system	system	NN
cord-288669-46tkedw7	209	18	.	.	.
cord-288669-46tkedw7	210	1	When	when	WRB
cord-288669-46tkedw7	210	2	the	the	DT
cord-288669-46tkedw7	210	3	inner	inner	JJ
cord-288669-46tkedw7	210	4	bacterial	bacterial	JJ
cord-288669-46tkedw7	210	5	membrane	membrane	NN
cord-288669-46tkedw7	210	6	is	be	VBZ
cord-288669-46tkedw7	210	7	intact	intact	JJ
cord-288669-46tkedw7	210	8	,	,	,
cord-288669-46tkedw7	210	9	intracellular	intracellular	JJ
cord-288669-46tkedw7	210	10	lysozymes	lysozyme	NNS
cord-288669-46tkedw7	210	11	can	can	MD
cord-288669-46tkedw7	210	12	not	not	RB
cord-288669-46tkedw7	210	13	reach	reach	VB
cord-288669-46tkedw7	210	14	the	the	DT
cord-288669-46tkedw7	210	15	cell	cell	NN
cord-288669-46tkedw7	210	16	wall	wall	NN
cord-288669-46tkedw7	210	17	.	.	.
cord-288669-46tkedw7	211	1	However	however	RB
cord-288669-46tkedw7	211	2	,	,	,
cord-288669-46tkedw7	211	3	permeabilized	permeabilized	JJ
cord-288669-46tkedw7	211	4	membranes	membrane	NNS
cord-288669-46tkedw7	211	5	allow	allow	VBP
cord-288669-46tkedw7	211	6	the	the	DT
cord-288669-46tkedw7	211	7	lysozymes	lysozyme	NNS
cord-288669-46tkedw7	211	8	to	to	TO
cord-288669-46tkedw7	211	9	gain	gain	VB
cord-288669-46tkedw7	211	10	access	access	NN
cord-288669-46tkedw7	211	11	to	to	IN
cord-288669-46tkedw7	211	12	the	the	DT
cord-288669-46tkedw7	211	13	peptidoglycan	peptidoglycan	NN
cord-288669-46tkedw7	211	14	,	,	,
cord-288669-46tkedw7	211	15	leading	lead	VBG
cord-288669-46tkedw7	211	16	to	to	IN
cord-288669-46tkedw7	211	17	cell	cell	NN
cord-288669-46tkedw7	211	18	lysis	lysis	NN
cord-288669-46tkedw7	211	19	.	.	.
cord-288669-46tkedw7	212	1	This	this	DT
cord-288669-46tkedw7	212	2	approach	approach	NN
cord-288669-46tkedw7	212	3	has	have	VBZ
cord-288669-46tkedw7	212	4	been	be	VBN
cord-288669-46tkedw7	212	5	shown	show	VBN
cord-288669-46tkedw7	212	6	to	to	TO
cord-288669-46tkedw7	212	7	be	be	VB
cord-288669-46tkedw7	212	8	suitable	suitable	JJ
cord-288669-46tkedw7	212	9	as	as	IN
cord-288669-46tkedw7	212	10	a	a	DT
cord-288669-46tkedw7	212	11	permeabilization	permeabilization	NN
cord-288669-46tkedw7	212	12	test	test	NN
cord-288669-46tkedw7	212	13	of	of	IN
cord-288669-46tkedw7	212	14	the	the	DT
cord-288669-46tkedw7	212	15	inner	inner	JJ
cord-288669-46tkedw7	212	16	bacterial	bacterial	JJ
cord-288669-46tkedw7	212	17	membrane	membrane	NN
cord-288669-46tkedw7	212	18	for	for	IN
cord-288669-46tkedw7	212	19	viral	viral	JJ
cord-288669-46tkedw7	212	20	proteins	protein	NNS
cord-288669-46tkedw7	213	1	(	(	-LRB-
cord-288669-46tkedw7	213	2	Bodelón	Bodelón	NNP
cord-288669-46tkedw7	213	3	et	et	NNP
cord-288669-46tkedw7	213	4	al	al	NNP
cord-288669-46tkedw7	213	5	.	.	NNP
cord-288669-46tkedw7	213	6	,	,	,
cord-288669-46tkedw7	213	7	2002	2002	CD
cord-288669-46tkedw7	213	8	;	;	:
cord-288669-46tkedw7	213	9	Ciccaglione	Ciccaglione	NNP
cord-288669-46tkedw7	213	10	et	et	NNP
cord-288669-46tkedw7	213	11	al	al	NNP
cord-288669-46tkedw7	213	12	.	.	NNP
cord-288669-46tkedw7	213	13	,	,	,
cord-288669-46tkedw7	213	14	1998	1998	CD
cord-288669-46tkedw7	213	15	;	;	:
cord-288669-46tkedw7	214	1	Liao	Liao	NNP
cord-288669-46tkedw7	214	2	et	et	NNP
cord-288669-46tkedw7	214	3	al	al	NNP
cord-288669-46tkedw7	214	4	.	.	NNP
cord-288669-46tkedw7	214	5	,	,	,
cord-288669-46tkedw7	214	6	2004	2004	CD
cord-288669-46tkedw7	214	7	;	;	:
cord-288669-46tkedw7	214	8	Madan	Madan	NNP
cord-288669-46tkedw7	214	9	et	et	NNP
cord-288669-46tkedw7	214	10	al	al	NNP
cord-288669-46tkedw7	214	11	.	.	NNP
cord-288669-46tkedw7	214	12	,	,	,
cord-288669-46tkedw7	214	13	2005	2005	CD
cord-288669-46tkedw7	214	14	)	)	-RRB-
cord-288669-46tkedw7	214	15	.	.	.
cord-288669-46tkedw7	215	1	The	the	DT
cord-288669-46tkedw7	215	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	215	3	E	E	NNP
cord-288669-46tkedw7	215	4	protein	protein	NN
cord-288669-46tkedw7	215	5	gene	gene	NN
cord-288669-46tkedw7	215	6	was	be	VBD
cord-288669-46tkedw7	215	7	first	first	RB
cord-288669-46tkedw7	215	8	cloned	clone	VBN
cord-288669-46tkedw7	215	9	in	in	IN
cord-288669-46tkedw7	215	10	the	the	DT
cord-288669-46tkedw7	215	11	GST	gst	NN
cord-288669-46tkedw7	215	12	-	-	HYPH
cord-288669-46tkedw7	215	13	fusion	fusion	NN
cord-288669-46tkedw7	215	14	vector	vector	NN
cord-288669-46tkedw7	215	15	and	and	CC
cord-288669-46tkedw7	215	16	expressed	express	VBN
cord-288669-46tkedw7	215	17	in	in	IN
cord-288669-46tkedw7	215	18	Escherichia	Escherichia	NNP
cord-288669-46tkedw7	215	19	coli	coli	NNS
cord-288669-46tkedw7	215	20	.	.	.
cord-288669-46tkedw7	216	1	The	the	DT
cord-288669-46tkedw7	216	2	growth	growth	NN
cord-288669-46tkedw7	216	3	rates	rate	NNS
cord-288669-46tkedw7	216	4	of	of	IN
cord-288669-46tkedw7	216	5	transformed	transform	VBN
cord-288669-46tkedw7	216	6	cells	cell	NNS
cord-288669-46tkedw7	216	7	were	be	VBD
cord-288669-46tkedw7	216	8	analyzed	analyze	VBN
cord-288669-46tkedw7	216	9	spectrophotometrically	spectrophotometrically	RB
cord-288669-46tkedw7	216	10	.	.	.
cord-288669-46tkedw7	217	1	A	a	DT
cord-288669-46tkedw7	217	2	drastic	drastic	JJ
cord-288669-46tkedw7	217	3	arrest	arrest	NN
cord-288669-46tkedw7	217	4	of	of	IN
cord-288669-46tkedw7	217	5	cell	cell	NN
cord-288669-46tkedw7	217	6	growth	growth	NN
cord-288669-46tkedw7	217	7	was	be	VBD
cord-288669-46tkedw7	217	8	observed	observe	VBN
cord-288669-46tkedw7	217	9	in	in	IN
cord-288669-46tkedw7	217	10	the	the	DT
cord-288669-46tkedw7	217	11	E	e	NN
cord-288669-46tkedw7	217	12	gene	gene	NN
cord-288669-46tkedw7	217	13	-	-	HYPH
cord-288669-46tkedw7	217	14	transformed	transform	VBN
cord-288669-46tkedw7	217	15	cells	cell	NNS
cord-288669-46tkedw7	217	16	upon	upon	IN
cord-288669-46tkedw7	217	17	IPTG	IPTG	NNP
cord-288669-46tkedw7	217	18	induction	induction	NN
cord-288669-46tkedw7	217	19	,	,	,
cord-288669-46tkedw7	217	20	whereas	whereas	IN
cord-288669-46tkedw7	217	21	bacteria	bacteria	NNS
cord-288669-46tkedw7	217	22	carrying	carry	VBG
cord-288669-46tkedw7	217	23	the	the	DT
cord-288669-46tkedw7	217	24	empty	empty	JJ
cord-288669-46tkedw7	217	25	plasmid	plasmid	NN
cord-288669-46tkedw7	217	26	or	or	CC
cord-288669-46tkedw7	217	27	the	the	DT
cord-288669-46tkedw7	217	28	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	217	29	N	N	NNP
cord-288669-46tkedw7	217	30	gene	gene	NN
cord-288669-46tkedw7	217	31	had	have	VBD
cord-288669-46tkedw7	217	32	no	no	DT
cord-288669-46tkedw7	217	33	effects	effect	NNS
cord-288669-46tkedw7	217	34	on	on	IN
cord-288669-46tkedw7	217	35	their	-PRON-	PRP$
cord-288669-46tkedw7	217	36	growth	growth	NN
cord-288669-46tkedw7	217	37	during	during	IN
cord-288669-46tkedw7	217	38	the	the	DT
cord-288669-46tkedw7	217	39	300-min	300-min	CD
cord-288669-46tkedw7	217	40	period	period	NN
cord-288669-46tkedw7	217	41	following	follow	VBG
cord-288669-46tkedw7	217	42	induction	induction	NN
cord-288669-46tkedw7	217	43	(	(	-LRB-
cord-288669-46tkedw7	217	44	Fig	fig	NN
cord-288669-46tkedw7	217	45	.	.	.
cord-288669-46tkedw7	217	46	6A	6a	CD
cord-288669-46tkedw7	217	47	)	)	-RRB-
cord-288669-46tkedw7	217	48	.	.	.
cord-288669-46tkedw7	218	1	This	this	DT
cord-288669-46tkedw7	218	2	finding	finding	NN
cord-288669-46tkedw7	218	3	suggests	suggest	VBZ
cord-288669-46tkedw7	218	4	that	that	IN
cord-288669-46tkedw7	218	5	the	the	DT
cord-288669-46tkedw7	218	6	expression	expression	NN
cord-288669-46tkedw7	218	7	of	of	IN
cord-288669-46tkedw7	218	8	E	E	NNS
cord-288669-46tkedw7	218	9	affected	affect	VBD
cord-288669-46tkedw7	218	10	the	the	DT
cord-288669-46tkedw7	218	11	cell	cell	NN
cord-288669-46tkedw7	218	12	growth	growth	NN
cord-288669-46tkedw7	218	13	negatively	negatively	RB
cord-288669-46tkedw7	218	14	by	by	IN
cord-288669-46tkedw7	218	15	altering	alter	VBG
cord-288669-46tkedw7	218	16	the	the	DT
cord-288669-46tkedw7	218	17	membrane	membrane	NN
cord-288669-46tkedw7	218	18	permeability	permeability	NN
cord-288669-46tkedw7	218	19	to	to	IN
cord-288669-46tkedw7	218	20	intracellular	intracellular	JJ
cord-288669-46tkedw7	218	21	lysozyme	lysozyme	NNS
cord-288669-46tkedw7	218	22	.	.	.
cord-288669-46tkedw7	219	1	To	to	TO
cord-288669-46tkedw7	219	2	further	further	RB
cord-288669-46tkedw7	219	3	determine	determine	VB
cord-288669-46tkedw7	219	4	if	if	IN
cord-288669-46tkedw7	219	5	the	the	DT
cord-288669-46tkedw7	219	6	growth	growth	NN
cord-288669-46tkedw7	219	7	rate	rate	NN
cord-288669-46tkedw7	219	8	of	of	IN
cord-288669-46tkedw7	219	9	cells	cell	NNS
cord-288669-46tkedw7	219	10	expressing	express	VBG
cord-288669-46tkedw7	219	11	E	E	NNP
cord-288669-46tkedw7	219	12	was	be	VBD
cord-288669-46tkedw7	219	13	the	the	DT
cord-288669-46tkedw7	219	14	consequence	consequence	NN
cord-288669-46tkedw7	219	15	of	of	IN
cord-288669-46tkedw7	219	16	permeability	permeability	NN
cord-288669-46tkedw7	219	17	modification	modification	NN
cord-288669-46tkedw7	219	18	,	,	,
cord-288669-46tkedw7	219	19	a	a	DT
cord-288669-46tkedw7	219	20	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	219	21	B	B	NNP
cord-288669-46tkedw7	219	22	permeability	permeability	NN
cord-288669-46tkedw7	219	23	assay	assay	NN
cord-288669-46tkedw7	219	24	was	be	VBD
cord-288669-46tkedw7	219	25	carried	carry	VBN
cord-288669-46tkedw7	219	26	out	out	RP
cord-288669-46tkedw7	219	27	.	.	.
cord-288669-46tkedw7	220	1	Hygromycin	Hygromycin	NNP
cord-288669-46tkedw7	220	2	B	B	NNP
cord-288669-46tkedw7	220	3	is	be	VBZ
cord-288669-46tkedw7	220	4	normally	normally	RB
cord-288669-46tkedw7	220	5	impermeable	impermeable	JJ
cord-288669-46tkedw7	220	6	to	to	IN
cord-288669-46tkedw7	220	7	the	the	DT
cord-288669-46tkedw7	220	8	membrane	membrane	NN
cord-288669-46tkedw7	220	9	barrier	barrier	NN
cord-288669-46tkedw7	220	10	during	during	IN
cord-288669-46tkedw7	220	11	a	a	DT
cord-288669-46tkedw7	220	12	short	short	JJ
cord-288669-46tkedw7	220	13	period	period	NN
cord-288669-46tkedw7	220	14	of	of	IN
cord-288669-46tkedw7	220	15	time	time	NN
cord-288669-46tkedw7	220	16	at	at	IN
cord-288669-46tkedw7	220	17	low	low	JJ
cord-288669-46tkedw7	220	18	concentration	concentration	NN
cord-288669-46tkedw7	220	19	,	,	,
cord-288669-46tkedw7	220	20	but	but	CC
cord-288669-46tkedw7	220	21	it	-PRON-	PRP
cord-288669-46tkedw7	220	22	can	can	MD
cord-288669-46tkedw7	220	23	readily	readily	RB
cord-288669-46tkedw7	220	24	penetrate	penetrate	VB
cord-288669-46tkedw7	220	25	the	the	DT
cord-288669-46tkedw7	220	26	permeabilized	permeabilized	JJ
cord-288669-46tkedw7	220	27	membrane	membrane	NN
cord-288669-46tkedw7	220	28	to	to	TO
cord-288669-46tkedw7	220	29	cause	cause	VB
cord-288669-46tkedw7	220	30	strong	strong	JJ
cord-288669-46tkedw7	220	31	inhibition	inhibition	NN
cord-288669-46tkedw7	220	32	of	of	IN
cord-288669-46tkedw7	220	33	intracellular	intracellular	JJ
cord-288669-46tkedw7	220	34	protein	protein	NN
cord-288669-46tkedw7	220	35	synthesis	synthesis	NN
cord-288669-46tkedw7	220	36	.	.	.
cord-288669-46tkedw7	221	1	The	the	DT
cord-288669-46tkedw7	221	2	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	221	3	B	B	NNP
cord-288669-46tkedw7	221	4	permeability	permeability	NN
cord-288669-46tkedw7	221	5	assay	assay	NN
cord-288669-46tkedw7	221	6	therefore	therefore	RB
cord-288669-46tkedw7	221	7	is	be	VBZ
cord-288669-46tkedw7	221	8	widely	widely	RB
cord-288669-46tkedw7	221	9	used	use	VBN
cord-288669-46tkedw7	221	10	to	to	TO
cord-288669-46tkedw7	221	11	study	study	VB
cord-288669-46tkedw7	221	12	changes	change	NNS
cord-288669-46tkedw7	221	13	of	of	IN
cord-288669-46tkedw7	221	14	membrane	membrane	NN
cord-288669-46tkedw7	221	15	permeability	permeability	NN
cord-288669-46tkedw7	221	16	as	as	RB
cord-288669-46tkedw7	221	17	well	well	RB
cord-288669-46tkedw7	221	18	as	as	IN
cord-288669-46tkedw7	221	19	to	to	TO
cord-288669-46tkedw7	221	20	identify	identify	VB
cord-288669-46tkedw7	221	21	proteins	protein	NNS
cord-288669-46tkedw7	221	22	that	that	WDT
cord-288669-46tkedw7	221	23	can	can	MD
cord-288669-46tkedw7	221	24	form	form	VB
cord-288669-46tkedw7	221	25	pores	pore	NNS
cord-288669-46tkedw7	221	26	in	in	IN
cord-288669-46tkedw7	221	27	lipid	lipid	NN
cord-288669-46tkedw7	221	28	membranes	membrane	NNS
cord-288669-46tkedw7	221	29	(	(	-LRB-
cord-288669-46tkedw7	221	30	Aldabe	Aldabe	NNP
cord-288669-46tkedw7	221	31	et	et	NNP
cord-288669-46tkedw7	221	32	al	al	NNP
cord-288669-46tkedw7	221	33	.	.	NNP
cord-288669-46tkedw7	221	34	,	,	,
cord-288669-46tkedw7	221	35	1996	1996	CD
cord-288669-46tkedw7	221	36	;	;	:
cord-288669-46tkedw7	221	37	Arroyo	Arroyo	NNP
cord-288669-46tkedw7	221	38	et	et	NNP
cord-288669-46tkedw7	221	39	al	al	NNP
cord-288669-46tkedw7	221	40	.	.	NNP
cord-288669-46tkedw7	221	41	,	,	,
cord-288669-46tkedw7	221	42	1995	1995	CD
cord-288669-46tkedw7	221	43	;	;	:
cord-288669-46tkedw7	221	44	Bodelón	Bodelón	NNP
cord-288669-46tkedw7	221	45	et	et	NNP
cord-288669-46tkedw7	221	46	al	al	NNP
cord-288669-46tkedw7	221	47	.	.	.
cord-288669-46tkedw7	222	1	,	,	,
cord-288669-46tkedw7	222	2	2002	2002	CD
cord-288669-46tkedw7	222	3	;	;	:
cord-288669-46tkedw7	222	4	Chang	Chang	NNP
cord-288669-46tkedw7	222	5	et	et	NNP
cord-288669-46tkedw7	222	6	al	al	NNP
cord-288669-46tkedw7	222	7	.	.	.
cord-288669-46tkedw7	223	1	,	,	,
cord-288669-46tkedw7	223	2	1999	1999	CD
cord-288669-46tkedw7	223	3	;	;	:
cord-288669-46tkedw7	223	4	Ciccaglione	Ciccaglione	NNP
cord-288669-46tkedw7	223	5	et	et	NNP
cord-288669-46tkedw7	223	6	al	al	NNP
cord-288669-46tkedw7	223	7	.	.	NNP
cord-288669-46tkedw7	223	8	,	,	,
cord-288669-46tkedw7	223	9	1998	1998	CD
cord-288669-46tkedw7	223	10	;	;	:
cord-288669-46tkedw7	223	11	de	de	NNP
cord-288669-46tkedw7	223	12	Jong	Jong	NNP
cord-288669-46tkedw7	223	13	et	et	NNP
cord-288669-46tkedw7	223	14	al	al	NNP
cord-288669-46tkedw7	223	15	.	.	NNP
cord-288669-46tkedw7	223	16	,	,	,
cord-288669-46tkedw7	223	17	2003	2003	CD
cord-288669-46tkedw7	224	1	;	;	:
cord-288669-46tkedw7	224	2	Doedens	Doedens	NNP
cord-288669-46tkedw7	224	3	and	and	CC
cord-288669-46tkedw7	224	4	Kirkegaard	Kirkegaard	NNP
cord-288669-46tkedw7	224	5	,	,	,
cord-288669-46tkedw7	224	6	1995	1995	CD
cord-288669-46tkedw7	224	7	;	;	:
cord-288669-46tkedw7	224	8	Han	Han	NNP
cord-288669-46tkedw7	224	9	and	and	CC
cord-288669-46tkedw7	224	10	Harty	Harty	NNP
cord-288669-46tkedw7	224	11	,	,	,
cord-288669-46tkedw7	224	12	2004	2004	CD
cord-288669-46tkedw7	224	13	;	;	:
cord-288669-46tkedw7	224	14	Liao	Liao	NNP
cord-288669-46tkedw7	224	15	et	et	NNP
cord-288669-46tkedw7	224	16	al	al	NNP
cord-288669-46tkedw7	224	17	.	.	NNP
cord-288669-46tkedw7	224	18	,	,	,
cord-288669-46tkedw7	224	19	2004	2004	CD
cord-288669-46tkedw7	224	20	;	;	:
cord-288669-46tkedw7	224	21	Madan	Madan	NNP
cord-288669-46tkedw7	224	22	et	et	NNP
cord-288669-46tkedw7	224	23	al	al	NNP
cord-288669-46tkedw7	224	24	.	.	NNP
cord-288669-46tkedw7	224	25	,	,	,
cord-288669-46tkedw7	224	26	2005	2005	CD
cord-288669-46tkedw7	224	27	)	)	-RRB-
cord-288669-46tkedw7	224	28	.	.	.
cord-288669-46tkedw7	225	1	At	at	IN
cord-288669-46tkedw7	225	2	1	1	CD
cord-288669-46tkedw7	225	3	h	h	NN
cord-288669-46tkedw7	225	4	induction	induction	NN
cord-288669-46tkedw7	225	5	for	for	IN
cord-288669-46tkedw7	225	6	E	e	NN
cord-288669-46tkedw7	225	7	protein	protein	NN
cord-288669-46tkedw7	225	8	expression	expression	NN
cord-288669-46tkedw7	225	9	,	,	,
cord-288669-46tkedw7	225	10	hygromycin	hygromycin	NNP
cord-288669-46tkedw7	225	11	B	B	NNP
cord-288669-46tkedw7	225	12	was	be	VBD
cord-288669-46tkedw7	225	13	added	add	VBN
cord-288669-46tkedw7	225	14	to	to	IN
cord-288669-46tkedw7	225	15	the	the	DT
cord-288669-46tkedw7	225	16	culture	culture	NN
cord-288669-46tkedw7	225	17	media	medium	NNS
cord-288669-46tkedw7	225	18	.	.	.
cord-288669-46tkedw7	226	1	The	the	DT
cord-288669-46tkedw7	226	2	cultures	culture	NNS
cord-288669-46tkedw7	226	3	were	be	VBD
cord-288669-46tkedw7	226	4	further	further	RB
cord-288669-46tkedw7	226	5	incubated	incubate	VBN
cord-288669-46tkedw7	226	6	for	for	IN
cord-288669-46tkedw7	226	7	15	15	CD
cord-288669-46tkedw7	226	8	min	min	NN
cord-288669-46tkedw7	226	9	and	and	CC
cord-288669-46tkedw7	226	10	metabolically	metabolically	RB
cord-288669-46tkedw7	226	11	labeled	label	VBN
cord-288669-46tkedw7	226	12	with	with	IN
cord-288669-46tkedw7	226	13	[	[	-LRB-
cord-288669-46tkedw7	226	14	35	35	CD
cord-288669-46tkedw7	226	15	S]methionine	S]methionine	NNP
cord-288669-46tkedw7	226	16	/	/	SYM
cord-288669-46tkedw7	226	17	cysteine	cysteine	NN
cord-288669-46tkedw7	226	18	for	for	IN
cord-288669-46tkedw7	226	19	15	15	CD
cord-288669-46tkedw7	226	20	min	min	NN
cord-288669-46tkedw7	226	21	,	,	,
cord-288669-46tkedw7	226	22	followed	follow	VBN
cord-288669-46tkedw7	226	23	by	by	IN
cord-288669-46tkedw7	226	24	SDS	SDS	NNP
cord-288669-46tkedw7	226	25	-	-	HYPH
cord-288669-46tkedw7	226	26	PAGE	PAGE	NNP
cord-288669-46tkedw7	226	27	of	of	IN
cord-288669-46tkedw7	226	28	cell	cell	NN
cord-288669-46tkedw7	226	29	lysates	lysate	NNS
cord-288669-46tkedw7	226	30	.	.	.
cord-288669-46tkedw7	227	1	The	the	DT
cord-288669-46tkedw7	227	2	Coomassie	Coomassie	NNP
cord-288669-46tkedw7	227	3	blue	blue	JJ
cord-288669-46tkedw7	227	4	staining	staining	NN
cord-288669-46tkedw7	227	5	showed	show	VBD
cord-288669-46tkedw7	227	6	that	that	IN
cord-288669-46tkedw7	227	7	equal	equal	JJ
cord-288669-46tkedw7	227	8	amounts	amount	NNS
cord-288669-46tkedw7	227	9	of	of	IN
cord-288669-46tkedw7	227	10	proteins	protein	NNS
cord-288669-46tkedw7	227	11	were	be	VBD
cord-288669-46tkedw7	227	12	loaded	load	VBN
cord-288669-46tkedw7	227	13	on	on	IN
cord-288669-46tkedw7	227	14	the	the	DT
cord-288669-46tkedw7	227	15	gel	gel	NN
cord-288669-46tkedw7	227	16	(	(	-LRB-
cord-288669-46tkedw7	227	17	Fig	Fig	NNP
cord-288669-46tkedw7	227	18	.	.	.
cord-288669-46tkedw7	227	19	6B	6b	CD
cord-288669-46tkedw7	227	20	,	,	,
cord-288669-46tkedw7	227	21	left	left	JJ
cord-288669-46tkedw7	227	22	panel	panel	NN
cord-288669-46tkedw7	227	23	)	)	-RRB-
cord-288669-46tkedw7	227	24	.	.	.
cord-288669-46tkedw7	228	1	Autoradiography	Autoradiography	NNP
cord-288669-46tkedw7	228	2	indicated	indicate	VBD
cord-288669-46tkedw7	228	3	that	that	IN
cord-288669-46tkedw7	228	4	hygromycin	hygromycin	NNP
cord-288669-46tkedw7	228	5	B	B	NNP
cord-288669-46tkedw7	228	6	entered	enter	VBD
cord-288669-46tkedw7	228	7	cells	cell	NNS
cord-288669-46tkedw7	228	8	that	that	WDT
cord-288669-46tkedw7	228	9	expressed	express	VBD
cord-288669-46tkedw7	228	10	the	the	DT
cord-288669-46tkedw7	228	11	E	e	NN
cord-288669-46tkedw7	228	12	protein	protein	NN
cord-288669-46tkedw7	228	13	,	,	,
cord-288669-46tkedw7	228	14	and	and	CC
cord-288669-46tkedw7	228	15	protein	protein	NN
cord-288669-46tkedw7	228	16	synthesis	synthesis	NN
cord-288669-46tkedw7	228	17	was	be	VBD
cord-288669-46tkedw7	228	18	completely	completely	RB
cord-288669-46tkedw7	228	19	blocked	block	VBN
cord-288669-46tkedw7	228	20	in	in	IN
cord-288669-46tkedw7	228	21	those	those	DT
cord-288669-46tkedw7	228	22	cells	cell	NNS
cord-288669-46tkedw7	228	23	(	(	-LRB-
cord-288669-46tkedw7	228	24	Fig	Fig	NNP
cord-288669-46tkedw7	228	25	.	.	.
cord-288669-46tkedw7	228	26	6B	6b	CD
cord-288669-46tkedw7	228	27	,	,	,
cord-288669-46tkedw7	228	28	right	right	JJ
cord-288669-46tkedw7	228	29	panel	panel	NN
cord-288669-46tkedw7	228	30	,	,	,
cord-288669-46tkedw7	228	31	compare	compare	VB
cord-288669-46tkedw7	228	32	lanes	lane	NNS
cord-288669-46tkedw7	228	33	5	5	CD
cord-288669-46tkedw7	228	34	and	and	CC
cord-288669-46tkedw7	228	35	6	6	CD
cord-288669-46tkedw7	228	36	,	,	,
cord-288669-46tkedw7	228	37	lanes	lane	NNS
cord-288669-46tkedw7	228	38	7	7	CD
cord-288669-46tkedw7	228	39	and	and	CC
cord-288669-46tkedw7	228	40	8)	8)	NN
cord-288669-46tkedw7	228	41	.	.	.
cord-288669-46tkedw7	229	1	For	for	IN
cord-288669-46tkedw7	229	2	cells	cell	NNS
cord-288669-46tkedw7	229	3	expressing	express	VBG
cord-288669-46tkedw7	229	4	the	the	DT
cord-288669-46tkedw7	229	5	GST	GST	NNP
cord-288669-46tkedw7	229	6	or	or	CC
cord-288669-46tkedw7	229	7	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	229	8	N	N	NNP
cord-288669-46tkedw7	229	9	protein	protein	NN
cord-288669-46tkedw7	229	10	,	,	,
cord-288669-46tkedw7	229	11	hygromycin	hygromycin	NNP
cord-288669-46tkedw7	229	12	B	B	NNP
cord-288669-46tkedw7	229	13	did	do	VBD
cord-288669-46tkedw7	229	14	not	not	RB
cord-288669-46tkedw7	229	15	inhibit	inhibit	VB
cord-288669-46tkedw7	229	16	protein	protein	NN
cord-288669-46tkedw7	229	17	synthesis	synthesis	NN
cord-288669-46tkedw7	229	18	(	(	-LRB-
cord-288669-46tkedw7	229	19	compare	compare	VB
cord-288669-46tkedw7	229	20	lanes	lane	NNS
cord-288669-46tkedw7	229	21	1	1	CD
cord-288669-46tkedw7	229	22	and	and	CC
cord-288669-46tkedw7	229	23	2	2	CD
cord-288669-46tkedw7	229	24	,	,	,
cord-288669-46tkedw7	229	25	lanes	lane	NNS
cord-288669-46tkedw7	229	26	 	 	_SP
cord-288669-46tkedw7	229	27	3	3	CD
cord-288669-46tkedw7	229	28	and	and	CC
cord-288669-46tkedw7	229	29	4	4	CD
cord-288669-46tkedw7	229	30	)	)	-RRB-
cord-288669-46tkedw7	229	31	.	.	.
cord-288669-46tkedw7	230	1	These	these	DT
cord-288669-46tkedw7	230	2	results	result	NNS
cord-288669-46tkedw7	230	3	indicate	indicate	VBP
cord-288669-46tkedw7	230	4	that	that	IN
cord-288669-46tkedw7	230	5	the	the	DT
cord-288669-46tkedw7	230	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	230	7	E	E	NNP
cord-288669-46tkedw7	230	8	protein	protein	NN
cord-288669-46tkedw7	230	9	enhanced	enhance	VBD
cord-288669-46tkedw7	230	10	membrane	membrane	NN
cord-288669-46tkedw7	230	11	permeability	permeability	NN
cord-288669-46tkedw7	230	12	in	in	IN
cord-288669-46tkedw7	230	13	bacterial	bacterial	JJ
cord-288669-46tkedw7	230	14	cells	cell	NNS
cord-288669-46tkedw7	230	15	.	.	.
cord-288669-46tkedw7	231	1	A	a	DT
cord-288669-46tkedw7	231	2	second	second	JJ
cord-288669-46tkedw7	231	3	common	common	JJ
cord-288669-46tkedw7	231	4	property	property	NN
cord-288669-46tkedw7	231	5	of	of	IN
cord-288669-46tkedw7	231	6	viroporins	viroporin	NNS
cord-288669-46tkedw7	231	7	is	be	VBZ
cord-288669-46tkedw7	231	8	oligomerization	oligomerization	NN
cord-288669-46tkedw7	231	9	.	.	.
cord-288669-46tkedw7	232	1	The	the	DT
cord-288669-46tkedw7	232	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	232	3	E	E	NNP
cord-288669-46tkedw7	232	4	protein	protein	NN
cord-288669-46tkedw7	232	5	contains	contain	VBZ
cord-288669-46tkedw7	232	6	two	two	CD
cord-288669-46tkedw7	232	7	well	well	RB
cord-288669-46tkedw7	232	8	-	-	HYPH
cord-288669-46tkedw7	232	9	conserved	conserve	VBN
cord-288669-46tkedw7	232	10	cysteine	cysteine	NN
cord-288669-46tkedw7	232	11	residues	residue	NNS
cord-288669-46tkedw7	232	12	;	;	:
cord-288669-46tkedw7	232	13	however	however	RB
cord-288669-46tkedw7	232	14	,	,	,
cord-288669-46tkedw7	232	15	the	the	DT
cord-288669-46tkedw7	232	16	E	e	NN
cord-288669-46tkedw7	232	17	protein	protein	NN
cord-288669-46tkedw7	232	18	was	be	VBD
cord-288669-46tkedw7	232	19	shown	show	VBN
cord-288669-46tkedw7	232	20	not	not	RB
cord-288669-46tkedw7	232	21	to	to	TO
cord-288669-46tkedw7	232	22	form	form	VB
cord-288669-46tkedw7	232	23	a	a	DT
cord-288669-46tkedw7	232	24	disulfide	disulfide	NN
cord-288669-46tkedw7	232	25	-	-	HYPH
cord-288669-46tkedw7	232	26	linked	link	VBN
cord-288669-46tkedw7	232	27	homodimer	homodimer	RB
cord-288669-46tkedw7	232	28	.	.	.
cord-288669-46tkedw7	233	1	The	the	DT
cord-288669-46tkedw7	233	2	absence	absence	NN
cord-288669-46tkedw7	233	3	of	of	IN
cord-288669-46tkedw7	233	4	covalently	covalently	RB
cord-288669-46tkedw7	233	5	linked	link	VBN
cord-288669-46tkedw7	233	6	homodimers	homodimer	NNS
cord-288669-46tkedw7	233	7	of	of	IN
cord-288669-46tkedw7	233	8	E	E	NNP
cord-288669-46tkedw7	233	9	was	be	VBD
cord-288669-46tkedw7	233	10	confirmed	confirm	VBN
cord-288669-46tkedw7	233	11	using	use	VBG
cord-288669-46tkedw7	233	12	the	the	DT
cord-288669-46tkedw7	233	13	recombinant	recombinant	JJ
cord-288669-46tkedw7	233	14	E	e	NN
cord-288669-46tkedw7	233	15	protein	protein	NN
cord-288669-46tkedw7	233	16	expressed	express	VBN
cord-288669-46tkedw7	233	17	in	in	IN
cord-288669-46tkedw7	233	18	HeLa	HeLa	NNP
cord-288669-46tkedw7	233	19	cells	cell	NNS
cord-288669-46tkedw7	233	20	by	by	IN
cord-288669-46tkedw7	233	21	vTF7	vTF7	NNP
cord-288669-46tkedw7	233	22	-	-	HYPH
cord-288669-46tkedw7	233	23	3	3	CD
cord-288669-46tkedw7	233	24	vaccinia	vaccinia	NN
cord-288669-46tkedw7	233	25	virus	virus	NN
cord-288669-46tkedw7	233	26	(	(	-LRB-
cord-288669-46tkedw7	233	27	Fig	Fig	NNP
cord-288669-46tkedw7	233	28	.	.	.
cord-288669-46tkedw7	233	29	7A	7a	NN
cord-288669-46tkedw7	233	30	)	)	-RRB-
cord-288669-46tkedw7	233	31	.	.	.
cord-288669-46tkedw7	234	1	Although	although	IN
cord-288669-46tkedw7	234	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	234	3	N	N	NNP
cord-288669-46tkedw7	234	4	-	-	HYPH
cord-288669-46tkedw7	234	5	N	N	NNP
cord-288669-46tkedw7	234	6	dimers	dimer	NNS
cord-288669-46tkedw7	234	7	of	of	IN
cord-288669-46tkedw7	234	8	30	30	CD
cord-288669-46tkedw7	234	9	kDa	kDa	NNS
cord-288669-46tkedw7	234	10	were	be	VBD
cord-288669-46tkedw7	234	11	readily	readily	RB
cord-288669-46tkedw7	234	12	detected	detect	VBN
cord-288669-46tkedw7	234	13	in	in	IN
cord-288669-46tkedw7	234	14	cells	cell	NNS
cord-288669-46tkedw7	234	15	expressing	express	VBG
cord-288669-46tkedw7	234	16	the	the	DT
cord-288669-46tkedw7	234	17	N	N	NNP
cord-288669-46tkedw7	234	18	protein	protein	NN
cord-288669-46tkedw7	234	19	(	(	-LRB-
cord-288669-46tkedw7	234	20	lane	lane	NN
cord-288669-46tkedw7	234	21	1	1	CD
cord-288669-46tkedw7	234	22	)	)	-RRB-
cord-288669-46tkedw7	234	23	,	,	,
cord-288669-46tkedw7	234	24	no	no	DT
cord-288669-46tkedw7	234	25	band	band	NN
cord-288669-46tkedw7	234	26	corresponding	correspond	VBG
cord-288669-46tkedw7	234	27	to	to	IN
cord-288669-46tkedw7	234	28	the	the	DT
cord-288669-46tkedw7	234	29	predicted	predict	VBN
cord-288669-46tkedw7	234	30	dimeric	dimeric	JJ
cord-288669-46tkedw7	234	31	form	form	NN
cord-288669-46tkedw7	234	32	of	of	IN
cord-288669-46tkedw7	234	33	E	E	NNP
cord-288669-46tkedw7	234	34	was	be	VBD
cord-288669-46tkedw7	234	35	identified	identify	VBN
cord-288669-46tkedw7	234	36	in	in	IN
cord-288669-46tkedw7	234	37	non	non	JJ
cord-288669-46tkedw7	234	38	-	-	JJ
cord-288669-46tkedw7	234	39	reducing	reducing	JJ
cord-288669-46tkedw7	234	40	conditions	condition	NNS
cord-288669-46tkedw7	234	41	(	(	-LRB-
cord-288669-46tkedw7	234	42	lane	lane	NN
cord-288669-46tkedw7	234	43	3	3	CD
cord-288669-46tkedw7	234	44	)	)	-RRB-
cord-288669-46tkedw7	234	45	,	,	,
cord-288669-46tkedw7	234	46	concluding	conclude	VBG
cord-288669-46tkedw7	234	47	that	that	IN
cord-288669-46tkedw7	234	48	the	the	DT
cord-288669-46tkedw7	234	49	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	234	50	E	E	NNP
cord-288669-46tkedw7	234	51	protein	protein	NN
cord-288669-46tkedw7	234	52	does	do	VBZ
cord-288669-46tkedw7	234	53	not	not	RB
cord-288669-46tkedw7	234	54	undergo	undergo	VB
cord-288669-46tkedw7	234	55	cysteine	cysteine	NN
cord-288669-46tkedw7	234	56	-	-	HYPH
cord-288669-46tkedw7	234	57	linked	link	VBN
cord-288669-46tkedw7	234	58	homodimerization	homodimerization	NN
cord-288669-46tkedw7	234	59	.	.	.
cord-288669-46tkedw7	235	1	Because	because	IN
cord-288669-46tkedw7	235	2	the	the	DT
cord-288669-46tkedw7	235	3	E	e	NN
cord-288669-46tkedw7	235	4	protein	protein	NN
cord-288669-46tkedw7	235	5	may	may	MD
cord-288669-46tkedw7	235	6	form	form	VB
cord-288669-46tkedw7	235	7	a	a	DT
cord-288669-46tkedw7	235	8	non	non	JJ
cord-288669-46tkedw7	235	9	-	-	JJ
cord-288669-46tkedw7	235	10	covalent	covalent	JJ
cord-288669-46tkedw7	235	11	association	association	NN
cord-288669-46tkedw7	235	12	with	with	IN
cord-288669-46tkedw7	235	13	itself	-PRON-	PRP
cord-288669-46tkedw7	235	14	as	as	IN
cord-288669-46tkedw7	235	15	shown	show	VBN
cord-288669-46tkedw7	235	16	by	by	IN
cord-288669-46tkedw7	235	17	GST	GST	NNP
cord-288669-46tkedw7	235	18	-	-	HYPH
cord-288669-46tkedw7	235	19	pull	pull	VB
cord-288669-46tkedw7	235	20	down	down	RP
cord-288669-46tkedw7	235	21	assay	assay	NN
cord-288669-46tkedw7	235	22	,	,	,
cord-288669-46tkedw7	235	23	the	the	DT
cord-288669-46tkedw7	235	24	potential	potential	NN
cord-288669-46tkedw7	235	25	of	of	IN
cord-288669-46tkedw7	235	26	E	e	NN
cord-288669-46tkedw7	235	27	to	to	TO
cord-288669-46tkedw7	235	28	form	form	VB
cord-288669-46tkedw7	235	29	oligomers	oligomer	NNS
cord-288669-46tkedw7	235	30	was	be	VBD
cord-288669-46tkedw7	235	31	further	further	RB
cord-288669-46tkedw7	235	32	investigated	investigate	VBN
cord-288669-46tkedw7	235	33	by	by	IN
cord-288669-46tkedw7	235	34	cross	cross	NN
cord-288669-46tkedw7	235	35	-	-	JJ
cord-288669-46tkedw7	235	36	linking	link	VBG
cord-288669-46tkedw7	235	37	experiments	experiment	NNS
cord-288669-46tkedw7	235	38	in	in	IN
cord-288669-46tkedw7	235	39	the	the	DT
cord-288669-46tkedw7	235	40	presence	presence	NN
cord-288669-46tkedw7	235	41	and	and	CC
cord-288669-46tkedw7	235	42	absence	absence	NN
cord-288669-46tkedw7	235	43	of	of	IN
cord-288669-46tkedw7	235	44	other	other	JJ
cord-288669-46tkedw7	235	45	viral	viral	JJ
cord-288669-46tkedw7	235	46	constituents	constituent	NNS
cord-288669-46tkedw7	235	47	.	.	.
cord-288669-46tkedw7	236	1	Radiolabeled	Radiolabeled	NNP
cord-288669-46tkedw7	236	2	PRRSVinfected	prrsvinfected	JJ
cord-288669-46tkedw7	236	3	cells	cell	NNS
cord-288669-46tkedw7	236	4	were	be	VBD
cord-288669-46tkedw7	236	5	treated	treat	VBN
cord-288669-46tkedw7	236	6	with	with	IN
cord-288669-46tkedw7	236	7	the	the	DT
cord-288669-46tkedw7	236	8	membrane	membrane	NN
cord-288669-46tkedw7	236	9	-	-	HYPH
cord-288669-46tkedw7	236	10	permeable	permeable	JJ
cord-288669-46tkedw7	236	11	cross	cross	NN
cord-288669-46tkedw7	236	12	-	-	JJ
cord-288669-46tkedw7	236	13	linking	link	VBG
cord-288669-46tkedw7	236	14	reagent	reagent	NN
cord-288669-46tkedw7	236	15	DSP	DSP	NNP
cord-288669-46tkedw7	236	16	,	,	,
cord-288669-46tkedw7	236	17	and	and	CC
cord-288669-46tkedw7	236	18	the	the	DT
cord-288669-46tkedw7	236	19	cell	cell	NN
cord-288669-46tkedw7	236	20	lysates	lysate	NNS
cord-288669-46tkedw7	236	21	were	be	VBD
cord-288669-46tkedw7	236	22	immunoprecipitated	immunoprecipitate	VBN
cord-288669-46tkedw7	236	23	by	by	IN
cord-288669-46tkedw7	236	24	N	n	CD
cord-288669-46tkedw7	236	25	-	-	HYPH
cord-288669-46tkedw7	236	26	specific	specific	JJ
cord-288669-46tkedw7	236	27	MAb	MAb	NNS
cord-288669-46tkedw7	236	28	or	or	CC
cord-288669-46tkedw7	236	29	E	e	NN
cord-288669-46tkedw7	236	30	-	-	JJ
cord-288669-46tkedw7	236	31	specific	specific	JJ
cord-288669-46tkedw7	236	32	antiserum	antiserum	NN
cord-288669-46tkedw7	236	33	followed	follow	VBN
cord-288669-46tkedw7	236	34	by	by	IN
cord-288669-46tkedw7	236	35	SDS	SDS	NNP
cord-288669-46tkedw7	236	36	-	-	HYPH
cord-288669-46tkedw7	236	37	PAGE	PAGE	NNP
cord-288669-46tkedw7	236	38	under	under	IN
cord-288669-46tkedw7	236	39	non	non	JJ
cord-288669-46tkedw7	236	40	-	-	JJ
cord-288669-46tkedw7	236	41	reducing	reducing	JJ
cord-288669-46tkedw7	236	42	conditions	condition	NNS
cord-288669-46tkedw7	237	1	(	(	-LRB-
cord-288669-46tkedw7	237	2	Fig	Fig	NNP
cord-288669-46tkedw7	237	3	.	.	.
cord-288669-46tkedw7	237	4	 	 	_SP
cord-288669-46tkedw7	237	5	7B	7B	NNP
cord-288669-46tkedw7	237	6	)	)	-RRB-
cord-288669-46tkedw7	237	7	.	.	.
cord-288669-46tkedw7	238	1	The	the	DT
cord-288669-46tkedw7	239	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	239	2	N	N	NNP
cord-288669-46tkedw7	239	3	protein	protein	NN
cord-288669-46tkedw7	239	4	formed	form	VBD
cord-288669-46tkedw7	239	5	a	a	DT
cord-288669-46tkedw7	239	6	number	number	NN
cord-288669-46tkedw7	239	7	of	of	IN
cord-288669-46tkedw7	239	8	higher	high	JJR
cord-288669-46tkedw7	239	9	-	-	HYPH
cord-288669-46tkedw7	239	10	order	order	NN
cord-288669-46tkedw7	239	11	oligomers	oligomer	NNS
cord-288669-46tkedw7	239	12	(	(	-LRB-
cord-288669-46tkedw7	239	13	lanes	lane	NNS
cord-288669-46tkedw7	239	14	1	1	CD
cord-288669-46tkedw7	239	15	,	,	,
cord-288669-46tkedw7	239	16	2	2	CD
cord-288669-46tkedw7	239	17	)	)	-RRB-
cord-288669-46tkedw7	239	18	,	,	,
cord-288669-46tkedw7	239	19	as	as	IN
cord-288669-46tkedw7	239	20	reported	report	VBN
cord-288669-46tkedw7	239	21	previously	previously	RB
cord-288669-46tkedw7	239	22	(	(	-LRB-
cord-288669-46tkedw7	239	23	Wootton	Wootton	NNP
cord-288669-46tkedw7	239	24	and	and	CC
cord-288669-46tkedw7	239	25	Yoo	Yoo	NNP
cord-288669-46tkedw7	239	26	,	,	,
cord-288669-46tkedw7	239	27	2003	2003	CD
cord-288669-46tkedw7	239	28	)	)	-RRB-
cord-288669-46tkedw7	239	29	.	.	.
cord-288669-46tkedw7	240	1	When	when	WRB
cord-288669-46tkedw7	240	2	the	the	DT
cord-288669-46tkedw7	240	3	E	e	NN
cord-288669-46tkedw7	240	4	protein	protein	NN
cord-288669-46tkedw7	240	5	in	in	IN
cord-288669-46tkedw7	240	6	virus	virus	NN
cord-288669-46tkedw7	240	7	-	-	HYPH
cord-288669-46tkedw7	240	8	infected	infect	VBN
cord-288669-46tkedw7	240	9	cells	cell	NNS
cord-288669-46tkedw7	240	10	was	be	VBD
cord-288669-46tkedw7	240	11	subjected	subject	VBN
cord-288669-46tkedw7	240	12	to	to	TO
cord-288669-46tkedw7	240	13	cross	cross	VB
cord-288669-46tkedw7	240	14	-	-	JJ
cord-288669-46tkedw7	240	15	linking	linking	JJ
cord-288669-46tkedw7	240	16	,	,	,
cord-288669-46tkedw7	240	17	numerous	numerous	JJ
cord-288669-46tkedw7	240	18	multimeric	multimeric	JJ
cord-288669-46tkedw7	240	19	forms	form	NNS
cord-288669-46tkedw7	240	20	of	of	IN
cord-288669-46tkedw7	240	21	E	e	NN
cord-288669-46tkedw7	240	22	protein	protein	NN
cord-288669-46tkedw7	240	23	were	be	VBD
cord-288669-46tkedw7	240	24	identified	identify	VBN
cord-288669-46tkedw7	240	25	(	(	-LRB-
cord-288669-46tkedw7	240	26	lane	lane	NN
cord-288669-46tkedw7	240	27	3	3	CD
cord-288669-46tkedw7	240	28	)	)	-RRB-
cord-288669-46tkedw7	240	29	.	.	.
cord-288669-46tkedw7	241	1	The	the	DT
cord-288669-46tkedw7	241	2	E	e	NN
cord-288669-46tkedw7	241	3	protein	protein	NN
cord-288669-46tkedw7	241	4	multimerization	multimerization	NN
cord-288669-46tkedw7	241	5	also	also	RB
cord-288669-46tkedw7	241	6	was	be	VBD
cord-288669-46tkedw7	241	7	examined	examine	VBN
cord-288669-46tkedw7	241	8	in	in	IN
cord-288669-46tkedw7	241	9	the	the	DT
cord-288669-46tkedw7	241	10	absence	absence	NN
cord-288669-46tkedw7	241	11	of	of	IN
cord-288669-46tkedw7	241	12	other	other	JJ
cord-288669-46tkedw7	241	13	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	241	14	proteins	protein	NNS
cord-288669-46tkedw7	241	15	in	in	IN
cord-288669-46tkedw7	241	16	HeLa	HeLa	NNP
cord-288669-46tkedw7	241	17	cells	cell	NNS
cord-288669-46tkedw7	241	18	expressing	express	VBG
cord-288669-46tkedw7	241	19	E	e	NN
cord-288669-46tkedw7	241	20	protein	protein	NN
cord-288669-46tkedw7	241	21	by	by	IN
cord-288669-46tkedw7	241	22	T7	T7	NNP
cord-288669-46tkedw7	241	23	vaccinia	vaccinia	NN
cord-288669-46tkedw7	241	24	virus	virus	NN
cord-288669-46tkedw7	241	25	,	,	,
cord-288669-46tkedw7	241	26	and	and	CC
cord-288669-46tkedw7	241	27	again	again	RB
cord-288669-46tkedw7	241	28	higher	high	JJR
cord-288669-46tkedw7	241	29	-	-	HYPH
cord-288669-46tkedw7	241	30	order	order	NN
cord-288669-46tkedw7	241	31	multimeric	multimeric	JJ
cord-288669-46tkedw7	241	32	forms	form	NNS
cord-288669-46tkedw7	241	33	of	of	IN
cord-288669-46tkedw7	241	34	the	the	DT
cord-288669-46tkedw7	241	35	E	e	NN
cord-288669-46tkedw7	241	36	protein	protein	NN
cord-288669-46tkedw7	241	37	were	be	VBD
cord-288669-46tkedw7	241	38	observed	observe	VBN
cord-288669-46tkedw7	241	39	(	(	-LRB-
cord-288669-46tkedw7	241	40	lane	lane	NN
cord-288669-46tkedw7	241	41	4	4	CD
cord-288669-46tkedw7	241	42	)	)	-RRB-
cord-288669-46tkedw7	241	43	,	,	,
cord-288669-46tkedw7	241	44	indicating	indicate	VBG
cord-288669-46tkedw7	241	45	that	that	IN
cord-288669-46tkedw7	241	46	oligomerization	oligomerization	NN
cord-288669-46tkedw7	241	47	of	of	IN
cord-288669-46tkedw7	241	48	E	E	NNP
cord-288669-46tkedw7	241	49	is	be	VBZ
cord-288669-46tkedw7	241	50	independent	independent	JJ
cord-288669-46tkedw7	241	51	of	of	IN
cord-288669-46tkedw7	241	52	other	other	JJ
cord-288669-46tkedw7	241	53	viral	viral	JJ
cord-288669-46tkedw7	241	54	proteins	protein	NNS
cord-288669-46tkedw7	241	55	.	.	.
cord-288669-46tkedw7	242	1	Altogether	altogether	RB
cord-288669-46tkedw7	242	2	,	,	,
cord-288669-46tkedw7	242	3	our	-PRON-	PRP$
cord-288669-46tkedw7	242	4	data	datum	NNS
cord-288669-46tkedw7	242	5	indicate	indicate	VBP
cord-288669-46tkedw7	242	6	that	that	IN
cord-288669-46tkedw7	242	7	the	the	DT
cord-288669-46tkedw7	242	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	242	9	E	E	NNP
cord-288669-46tkedw7	242	10	protein	protein	NN
cord-288669-46tkedw7	242	11	exists	exist	VBZ
cord-288669-46tkedw7	242	12	as	as	IN
cord-288669-46tkedw7	242	13	non	non	JJ
cord-288669-46tkedw7	242	14	-	-	JJ
cord-288669-46tkedw7	242	15	covalently	covalently	RB
cord-288669-46tkedw7	242	16	linked	link	VBN
cord-288669-46tkedw7	242	17	oligomers	oligomer	NNS
cord-288669-46tkedw7	242	18	in	in	IN
cord-288669-46tkedw7	242	19	virus	virus	NN
cord-288669-46tkedw7	242	20	-	-	HYPH
cord-288669-46tkedw7	242	21	infected	infect	VBN
cord-288669-46tkedw7	242	22	cells	cell	NNS
cord-288669-46tkedw7	242	23	and	and	CC
cord-288669-46tkedw7	242	24	suggest	suggest	VBP
cord-288669-46tkedw7	242	25	that	that	IN
cord-288669-46tkedw7	242	26	the	the	DT
cord-288669-46tkedw7	242	27	multimerization	multimerization	NN
cord-288669-46tkedw7	242	28	may	may	MD
cord-288669-46tkedw7	242	29	be	be	VB
cord-288669-46tkedw7	242	30	the	the	DT
cord-288669-46tkedw7	242	31	physical	physical	JJ
cord-288669-46tkedw7	242	32	basis	basis	NN
cord-288669-46tkedw7	242	33	for	for	IN
cord-288669-46tkedw7	242	34	viroporin	viroporin	NN
cord-288669-46tkedw7	242	35	formation	formation	NN
cord-288669-46tkedw7	242	36	of	of	IN
cord-288669-46tkedw7	242	37	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	242	38	E.	E.	NNP
cord-288669-46tkedw7	243	1	The	the	DT
cord-288669-46tkedw7	243	2	present	present	JJ
cord-288669-46tkedw7	243	3	study	study	NN
cord-288669-46tkedw7	243	4	was	be	VBD
cord-288669-46tkedw7	243	5	conducted	conduct	VBN
cord-288669-46tkedw7	243	6	to	to	TO
cord-288669-46tkedw7	243	7	investigate	investigate	VB
cord-288669-46tkedw7	243	8	the	the	DT
cord-288669-46tkedw7	243	9	role	role	NN
cord-288669-46tkedw7	243	10	of	of	IN
cord-288669-46tkedw7	243	11	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	243	12	E	E	NNP
cord-288669-46tkedw7	243	13	protein	protein	NN
cord-288669-46tkedw7	243	14	during	during	IN
cord-288669-46tkedw7	243	15	virus	virus	NN
cord-288669-46tkedw7	243	16	infection	infection	NN
cord-288669-46tkedw7	243	17	.	.	.
cord-288669-46tkedw7	244	1	A	a	DT
cord-288669-46tkedw7	244	2	reverse	reverse	JJ
cord-288669-46tkedw7	244	3	genetics	genetics	NN
cord-288669-46tkedw7	244	4	approach	approach	NN
cord-288669-46tkedw7	244	5	was	be	VBD
cord-288669-46tkedw7	244	6	used	use	VBN
cord-288669-46tkedw7	244	7	to	to	TO
cord-288669-46tkedw7	244	8	modify	modify	VB
cord-288669-46tkedw7	244	9	the	the	DT
cord-288669-46tkedw7	244	10	translation	translation	NN
cord-288669-46tkedw7	244	11	initiation	initiation	NN
cord-288669-46tkedw7	244	12	of	of	IN
cord-288669-46tkedw7	244	13	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	244	14	,	,	,
cord-288669-46tkedw7	244	15	so	so	IN
cord-288669-46tkedw7	244	16	that	that	IN
cord-288669-46tkedw7	244	17	the	the	DT
cord-288669-46tkedw7	244	18	modified	modify	VBN
cord-288669-46tkedw7	244	19	genome	genome	NN
cord-288669-46tkedw7	244	20	was	be	VBD
cord-288669-46tkedw7	244	21	unable	unable	JJ
cord-288669-46tkedw7	244	22	to	to	TO
cord-288669-46tkedw7	244	23	express	express	VB
cord-288669-46tkedw7	244	24	the	the	DT
cord-288669-46tkedw7	244	25	E	e	NN
cord-288669-46tkedw7	244	26	protein	protein	NN
cord-288669-46tkedw7	244	27	.	.	.
cord-288669-46tkedw7	245	1	Our	-PRON-	PRP$
cord-288669-46tkedw7	245	2	experiments	experiment	NNS
cord-288669-46tkedw7	245	3	show	show	VBP
cord-288669-46tkedw7	245	4	that	that	IN
cord-288669-46tkedw7	245	5	the	the	DT
cord-288669-46tkedw7	245	6	absence	absence	NN
cord-288669-46tkedw7	245	7	of	of	IN
cord-288669-46tkedw7	245	8	E	e	NN
cord-288669-46tkedw7	245	9	protein	protein	NN
cord-288669-46tkedw7	245	10	expression	expression	NN
cord-288669-46tkedw7	245	11	does	do	VBZ
cord-288669-46tkedw7	245	12	not	not	RB
cord-288669-46tkedw7	245	13	affect	affect	VB
cord-288669-46tkedw7	245	14	genome	genome	NN
cord-288669-46tkedw7	245	15	replication	replication	NN
cord-288669-46tkedw7	245	16	transcription	transcription	NN
cord-288669-46tkedw7	245	17	but	but	CC
cord-288669-46tkedw7	245	18	impairs	impair	VBZ
cord-288669-46tkedw7	245	19	the	the	DT
cord-288669-46tkedw7	245	20	production	production	NN
cord-288669-46tkedw7	245	21	of	of	IN
cord-288669-46tkedw7	245	22	infectious	infectious	JJ
cord-288669-46tkedw7	245	23	virus	virus	NN
cord-288669-46tkedw7	245	24	.	.	.
cord-288669-46tkedw7	246	1	These	these	DT
cord-288669-46tkedw7	246	2	data	datum	NNS
cord-288669-46tkedw7	246	3	indicate	indicate	VBP
cord-288669-46tkedw7	246	4	that	that	IN
cord-288669-46tkedw7	246	5	the	the	DT
cord-288669-46tkedw7	246	6	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	246	7	E	E	NNP
cord-288669-46tkedw7	246	8	protein	protein	NN
cord-288669-46tkedw7	246	9	is	be	VBZ
cord-288669-46tkedw7	246	10	an	an	DT
cord-288669-46tkedw7	246	11	essential	essential	JJ
cord-288669-46tkedw7	246	12	structural	structural	JJ
cord-288669-46tkedw7	246	13	component	component	NN
cord-288669-46tkedw7	246	14	for	for	IN
cord-288669-46tkedw7	246	15	infectivity	infectivity	NN
cord-288669-46tkedw7	246	16	.	.	.
cord-288669-46tkedw7	247	1	The	the	DT
cord-288669-46tkedw7	247	2	E	e	NN
cord-288669-46tkedw7	247	3	protein	protein	NN
cord-288669-46tkedw7	247	4	however	however	RB
cord-288669-46tkedw7	247	5	appears	appear	VBZ
cord-288669-46tkedw7	247	6	not	not	RB
cord-288669-46tkedw7	247	7	to	to	TO
cord-288669-46tkedw7	247	8	be	be	VB
cord-288669-46tkedw7	247	9	an	an	DT
cord-288669-46tkedw7	247	10	essential	essential	JJ
cord-288669-46tkedw7	247	11	component	component	NN
cord-288669-46tkedw7	247	12	for	for	IN
cord-288669-46tkedw7	247	13	particle	particle	NN
cord-288669-46tkedw7	247	14	assembly	assembly	NN
cord-288669-46tkedw7	247	15	nor	nor	CC
cord-288669-46tkedw7	247	16	genomic	genomic	JJ
cord-288669-46tkedw7	247	17	encapsidation	encapsidation	NN
cord-288669-46tkedw7	247	18	.	.	.
cord-288669-46tkedw7	248	1	The	the	DT
cord-288669-46tkedw7	248	2	virion	virion	NN
cord-288669-46tkedw7	248	3	particles	particle	NNS
cord-288669-46tkedw7	248	4	devoid	devoid	JJ
cord-288669-46tkedw7	248	5	of	of	IN
cord-288669-46tkedw7	248	6	E	e	NN
cord-288669-46tkedw7	248	7	still	still	RB
cord-288669-46tkedw7	248	8	contained	contain	VBD
cord-288669-46tkedw7	248	9	the	the	DT
cord-288669-46tkedw7	248	10	viral	viral	JJ
cord-288669-46tkedw7	248	11	genome	genome	NN
cord-288669-46tkedw7	248	12	and	and	CC
cord-288669-46tkedw7	248	13	had	have	VBD
cord-288669-46tkedw7	248	14	a	a	DT
cord-288669-46tkedw7	248	15	similar	similar	JJ
cord-288669-46tkedw7	248	16	appearance	appearance	NN
cord-288669-46tkedw7	248	17	to	to	IN
cord-288669-46tkedw7	248	18	that	that	DT
cord-288669-46tkedw7	248	19	of	of	IN
cord-288669-46tkedw7	248	20	wildtype	wildtype	NN
cord-288669-46tkedw7	248	21	virions	virion	NNS
cord-288669-46tkedw7	248	22	.	.	.
cord-288669-46tkedw7	249	1	For	for	IN
cord-288669-46tkedw7	249	2	infection	infection	NN
cord-288669-46tkedw7	249	3	,	,	,
cord-288669-46tkedw7	249	4	virus	virus	NN
cord-288669-46tkedw7	249	5	particles	particle	NNS
cord-288669-46tkedw7	249	6	must	must	MD
cord-288669-46tkedw7	249	7	proceed	proceed	VB
cord-288669-46tkedw7	249	8	through	through	IN
cord-288669-46tkedw7	249	9	a	a	DT
cord-288669-46tkedw7	249	10	multiple	multiple	JJ
cord-288669-46tkedw7	249	11	-	-	HYPH
cord-288669-46tkedw7	249	12	step	step	NN
cord-288669-46tkedw7	249	13	cycle	cycle	NN
cord-288669-46tkedw7	249	14	of	of	IN
cord-288669-46tkedw7	249	15	entry	entry	NN
cord-288669-46tkedw7	249	16	and	and	CC
cord-288669-46tkedw7	249	17	uncoating	uncoating	NN
cord-288669-46tkedw7	249	18	,	,	,
cord-288669-46tkedw7	249	19	replication	replication	NN
cord-288669-46tkedw7	249	20	and	and	CC
cord-288669-46tkedw7	249	21	transcription	transcription	NN
cord-288669-46tkedw7	249	22	and	and	CC
cord-288669-46tkedw7	249	23	assembly	assembly	NN
cord-288669-46tkedw7	249	24	and	and	CC
cord-288669-46tkedw7	249	25	release	release	NN
cord-288669-46tkedw7	249	26	.	.	.
cord-288669-46tkedw7	250	1	According	accord	VBG
cord-288669-46tkedw7	250	2	to	to	IN
cord-288669-46tkedw7	250	3	our	-PRON-	PRP$
cord-288669-46tkedw7	250	4	findings	finding	NNS
cord-288669-46tkedw7	250	5	,	,	,
cord-288669-46tkedw7	250	6	the	the	DT
cord-288669-46tkedw7	250	7	E	e	NN
cord-288669-46tkedw7	250	8	protein	protein	NN
cord-288669-46tkedw7	250	9	does	do	VBZ
cord-288669-46tkedw7	250	10	not	not	RB
cord-288669-46tkedw7	250	11	exert	exert	VB
cord-288669-46tkedw7	250	12	an	an	DT
cord-288669-46tkedw7	250	13	important	important	JJ
cord-288669-46tkedw7	250	14	function	function	NN
cord-288669-46tkedw7	250	15	at	at	IN
cord-288669-46tkedw7	250	16	later	later	JJ
cord-288669-46tkedw7	250	17	events	event	NNS
cord-288669-46tkedw7	250	18	in	in	IN
cord-288669-46tkedw7	250	19	the	the	DT
cord-288669-46tkedw7	250	20	infection	infection	NN
cord-288669-46tkedw7	250	21	process	process	NN
cord-288669-46tkedw7	250	22	such	such	JJ
cord-288669-46tkedw7	250	23	as	as	IN
cord-288669-46tkedw7	250	24	genome	genome	NN
cord-288669-46tkedw7	250	25	replication	replication	NN
cord-288669-46tkedw7	250	26	,	,	,
cord-288669-46tkedw7	250	27	particle	particle	NN
cord-288669-46tkedw7	250	28	assembly	assembly	NN
cord-288669-46tkedw7	250	29	and	and	CC
cord-288669-46tkedw7	250	30	release	release	NN
cord-288669-46tkedw7	250	31	of	of	IN
cord-288669-46tkedw7	250	32	virus	virus	NN
cord-288669-46tkedw7	250	33	.	.	.
cord-288669-46tkedw7	251	1	We	-PRON-	PRP
cord-288669-46tkedw7	251	2	have	have	VBP
cord-288669-46tkedw7	251	3	observed	observe	VBN
cord-288669-46tkedw7	251	4	that	that	IN
cord-288669-46tkedw7	251	5	the	the	DT
cord-288669-46tkedw7	251	6	E	e	NN
cord-288669-46tkedw7	251	7	protein	protein	NN
cord-288669-46tkedw7	251	8	-	-	HYPH
cord-288669-46tkedw7	251	9	negative	negative	JJ
cord-288669-46tkedw7	251	10	,	,	,
cord-288669-46tkedw7	251	11	non	non	JJ
cord-288669-46tkedw7	251	12	-	-	JJ
cord-288669-46tkedw7	251	13	infectious	infectious	JJ
cord-288669-46tkedw7	251	14	virion	virion	NN
cord-288669-46tkedw7	251	15	particles	particle	NNS
cord-288669-46tkedw7	251	16	are	be	VBP
cord-288669-46tkedw7	251	17	able	able	JJ
cord-288669-46tkedw7	251	18	to	to	TO
cord-288669-46tkedw7	251	19	enter	enter	VB
cord-288669-46tkedw7	251	20	cells	cell	NNS
cord-288669-46tkedw7	251	21	but	but	CC
cord-288669-46tkedw7	251	22	subsequent	subsequent	JJ
cord-288669-46tkedw7	251	23	steps	step	NNS
cord-288669-46tkedw7	251	24	of	of	IN
cord-288669-46tkedw7	251	25	replication	replication	NN
cord-288669-46tkedw7	251	26	are	be	VBP
cord-288669-46tkedw7	251	27	inhibited	inhibit	VBN
cord-288669-46tkedw7	251	28	.	.	.
cord-288669-46tkedw7	252	1	This	this	DT
cord-288669-46tkedw7	252	2	suggests	suggest	VBZ
cord-288669-46tkedw7	252	3	that	that	IN
cord-288669-46tkedw7	252	4	the	the	DT
cord-288669-46tkedw7	252	5	particles	particle	NNS
cord-288669-46tkedw7	252	6	lacking	lack	VBG
cord-288669-46tkedw7	252	7	the	the	DT
cord-288669-46tkedw7	252	8	E	e	NN
cord-288669-46tkedw7	252	9	protein	protein	NN
cord-288669-46tkedw7	252	10	most	most	RBS
cord-288669-46tkedw7	252	11	likely	likely	RB
cord-288669-46tkedw7	252	12	remain	remain	VBP
cord-288669-46tkedw7	252	13	in	in	IN
cord-288669-46tkedw7	252	14	the	the	DT
cord-288669-46tkedw7	252	15	endosome	endosome	NN
cord-288669-46tkedw7	252	16	and	and	CC
cord-288669-46tkedw7	252	17	the	the	DT
cord-288669-46tkedw7	252	18	viral	viral	JJ
cord-288669-46tkedw7	252	19	genome	genome	NN
cord-288669-46tkedw7	252	20	is	be	VBZ
cord-288669-46tkedw7	252	21	not	not	RB
cord-288669-46tkedw7	252	22	released	release	VBN
cord-288669-46tkedw7	252	23	,	,	,
cord-288669-46tkedw7	252	24	which	which	WDT
cord-288669-46tkedw7	252	25	is	be	VBZ
cord-288669-46tkedw7	252	26	the	the	DT
cord-288669-46tkedw7	252	27	step	step	NN
cord-288669-46tkedw7	252	28	between	between	IN
cord-288669-46tkedw7	252	29	virus	virus	NN
cord-288669-46tkedw7	252	30	entry	entry	NN
cord-288669-46tkedw7	252	31	and	and	CC
cord-288669-46tkedw7	252	32	genome	genome	NN
cord-288669-46tkedw7	252	33	replication	replication	NN
cord-288669-46tkedw7	252	34	.	.	.
cord-288669-46tkedw7	253	1	The	the	DT
cord-288669-46tkedw7	253	2	uncoating	uncoating	NN
cord-288669-46tkedw7	253	3	is	be	VBZ
cord-288669-46tkedw7	253	4	a	a	DT
cord-288669-46tkedw7	253	5	critical	critical	JJ
cord-288669-46tkedw7	253	6	step	step	NN
cord-288669-46tkedw7	253	7	for	for	IN
cord-288669-46tkedw7	253	8	virus	virus	NN
cord-288669-46tkedw7	253	9	infection	infection	NN
cord-288669-46tkedw7	253	10	,	,	,
cord-288669-46tkedw7	253	11	during	during	IN
cord-288669-46tkedw7	253	12	which	which	WDT
cord-288669-46tkedw7	253	13	the	the	DT
cord-288669-46tkedw7	253	14	lipid	lipid	NN
cord-288669-46tkedw7	253	15	envelope	envelope	NN
cord-288669-46tkedw7	253	16	is	be	VBZ
cord-288669-46tkedw7	253	17	shed	shed	VBN
cord-288669-46tkedw7	253	18	and	and	CC
cord-288669-46tkedw7	253	19	the	the	DT
cord-288669-46tkedw7	253	20	viral	viral	JJ
cord-288669-46tkedw7	253	21	capsid	capsid	NN
cord-288669-46tkedw7	253	22	is	be	VBZ
cord-288669-46tkedw7	253	23	disassembled	disassemble	VBN
cord-288669-46tkedw7	253	24	to	to	TO
cord-288669-46tkedw7	253	25	release	release	VB
cord-288669-46tkedw7	253	26	the	the	DT
cord-288669-46tkedw7	253	27	genome	genome	NN
cord-288669-46tkedw7	253	28	to	to	TO
cord-288669-46tkedw7	253	29	initiate	initiate	VB
cord-288669-46tkedw7	253	30	a	a	DT
cord-288669-46tkedw7	253	31	replication	replication	NN
cord-288669-46tkedw7	253	32	cycle	cycle	NN
cord-288669-46tkedw7	253	33	in	in	IN
cord-288669-46tkedw7	253	34	the	the	DT
cord-288669-46tkedw7	253	35	cytoplasm	cytoplasm	NN
cord-288669-46tkedw7	253	36	.	.	.
cord-288669-46tkedw7	254	1	For	for	IN
cord-288669-46tkedw7	254	2	viruses	virus	NNS
cord-288669-46tkedw7	254	3	entering	enter	VBG
cord-288669-46tkedw7	254	4	cells	cell	NNS
cord-288669-46tkedw7	254	5	by	by	IN
cord-288669-46tkedw7	254	6	receptor	receptor	NN
cord-288669-46tkedw7	254	7	-	-	HYPH
cord-288669-46tkedw7	254	8	mediated	mediate	VBN
cord-288669-46tkedw7	254	9	endocytosis	endocytosis	NN
cord-288669-46tkedw7	254	10	,	,	,
cord-288669-46tkedw7	254	11	the	the	DT
cord-288669-46tkedw7	254	12	uncoating	uncoating	NN
cord-288669-46tkedw7	254	13	process	process	NN
cord-288669-46tkedw7	254	14	occurs	occur	VBZ
cord-288669-46tkedw7	254	15	in	in	IN
cord-288669-46tkedw7	254	16	the	the	DT
cord-288669-46tkedw7	254	17	endosome	endosome	NN
cord-288669-46tkedw7	254	18	where	where	WRB
cord-288669-46tkedw7	254	19	an	an	DT
cord-288669-46tkedw7	254	20	acidic	acidic	JJ
cord-288669-46tkedw7	254	21	environment	environment	NN
cord-288669-46tkedw7	254	22	triggers	trigger	VBZ
cord-288669-46tkedw7	254	23	fusion	fusion	NN
cord-288669-46tkedw7	254	24	between	between	IN
cord-288669-46tkedw7	254	25	the	the	DT
cord-288669-46tkedw7	254	26	viral	viral	JJ
cord-288669-46tkedw7	254	27	membrane	membrane	NN
cord-288669-46tkedw7	254	28	and	and	CC
cord-288669-46tkedw7	254	29	the	the	DT
cord-288669-46tkedw7	254	30	endosomal	endosomal	NNP
cord-288669-46tkedw7	254	31	membrane	membrane	NN
cord-288669-46tkedw7	254	32	(	(	-LRB-
cord-288669-46tkedw7	254	33	reviewed	review	VBN
cord-288669-46tkedw7	254	34	by	by	IN
cord-288669-46tkedw7	254	35	Smith	Smith	NNP
cord-288669-46tkedw7	254	36	and	and	CC
cord-288669-46tkedw7	254	37	Helenius	Helenius	NNP
cord-288669-46tkedw7	254	38	,	,	,
cord-288669-46tkedw7	254	39	2004	2004	CD
cord-288669-46tkedw7	254	40	)	)	-RRB-
cord-288669-46tkedw7	254	41	.	.	.
cord-288669-46tkedw7	255	1	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	255	2	enters	enter	VBZ
cord-288669-46tkedw7	255	3	cells	cell	NNS
cord-288669-46tkedw7	255	4	through	through	IN
cord-288669-46tkedw7	255	5	receptormediated	receptormediate	VBN
cord-288669-46tkedw7	255	6	microfilament	microfilament	NN
cord-288669-46tkedw7	255	7	-	-	HYPH
cord-288669-46tkedw7	255	8	dependent	dependent	JJ
cord-288669-46tkedw7	255	9	endocytosis	endocytosis	NN
cord-288669-46tkedw7	255	10	in	in	IN
cord-288669-46tkedw7	255	11	which	which	WDT
cord-288669-46tkedw7	255	12	a	a	DT
cord-288669-46tkedw7	255	13	low	low	JJ
cord-288669-46tkedw7	255	14	pH	ph	NN
cord-288669-46tkedw7	255	15	is	be	VBZ
cord-288669-46tkedw7	255	16	required	require	VBN
cord-288669-46tkedw7	255	17	to	to	TO
cord-288669-46tkedw7	255	18	trigger	trigger	VB
cord-288669-46tkedw7	255	19	fusion	fusion	NN
cord-288669-46tkedw7	255	20	for	for	IN
cord-288669-46tkedw7	255	21	proper	proper	JJ
cord-288669-46tkedw7	255	22	uncoating	uncoating	NN
cord-288669-46tkedw7	255	23	by	by	IN
cord-288669-46tkedw7	255	24	an	an	DT
cord-288669-46tkedw7	255	25	unknown	unknown	JJ
cord-288669-46tkedw7	255	26	mechanism	mechanism	NN
cord-288669-46tkedw7	255	27	(	(	-LRB-
cord-288669-46tkedw7	255	28	Kreutz	Kreutz	NNP
cord-288669-46tkedw7	255	29	and	and	CC
cord-288669-46tkedw7	255	30	Ackermann	Ackermann	NNP
cord-288669-46tkedw7	255	31	,	,	,
cord-288669-46tkedw7	255	32	1996	1996	CD
cord-288669-46tkedw7	255	33	;	;	:
cord-288669-46tkedw7	256	1	Nauwynck	Nauwynck	NNP
cord-288669-46tkedw7	256	2	et	et	NNP
cord-288669-46tkedw7	256	3	al	al	NNP
cord-288669-46tkedw7	256	4	.	.	NNP
cord-288669-46tkedw7	256	5	,	,	,
cord-288669-46tkedw7	256	6	1999	1999	CD
cord-288669-46tkedw7	256	7	)	)	-RRB-
cord-288669-46tkedw7	256	8	.	.	.
cord-288669-46tkedw7	257	1	It	-PRON-	PRP
cord-288669-46tkedw7	257	2	is	be	VBZ
cord-288669-46tkedw7	257	3	possible	possible	JJ
cord-288669-46tkedw7	257	4	that	that	IN
cord-288669-46tkedw7	257	5	an	an	DT
cord-288669-46tkedw7	257	6	acidic	acidic	JJ
cord-288669-46tkedw7	257	7	pH	ph	NN
cord-288669-46tkedw7	257	8	in	in	IN
cord-288669-46tkedw7	257	9	the	the	DT
cord-288669-46tkedw7	257	10	endosome	endosome	NN
cord-288669-46tkedw7	257	11	may	may	MD
cord-288669-46tkedw7	257	12	lead	lead	VB
cord-288669-46tkedw7	257	13	to	to	IN
cord-288669-46tkedw7	257	14	conformational	conformational	JJ
cord-288669-46tkedw7	257	15	changes	change	NNS
cord-288669-46tkedw7	257	16	of	of	IN
cord-288669-46tkedw7	257	17	viral	viral	JJ
cord-288669-46tkedw7	257	18	protein	protein	NN
cord-288669-46tkedw7	257	19	(	(	-LRB-
cord-288669-46tkedw7	257	20	s	s	NN
cord-288669-46tkedw7	257	21	)	)	-RRB-
cord-288669-46tkedw7	257	22	to	to	TO
cord-288669-46tkedw7	257	23	expose	expose	VB
cord-288669-46tkedw7	257	24	hidden	hide	VBN
cord-288669-46tkedw7	257	25	fusogenic	fusogenic	JJ
cord-288669-46tkedw7	257	26	domains	domain	NNS
cord-288669-46tkedw7	257	27	that	that	WDT
cord-288669-46tkedw7	257	28	facilitate	facilitate	VBP
cord-288669-46tkedw7	257	29	the	the	DT
cord-288669-46tkedw7	257	30	fusion	fusion	NN
cord-288669-46tkedw7	257	31	between	between	IN
cord-288669-46tkedw7	257	32	viral	viral	JJ
cord-288669-46tkedw7	257	33	and	and	CC
cord-288669-46tkedw7	257	34	endosomal	endosomal	JJ
cord-288669-46tkedw7	257	35	membranes	membrane	NNS
cord-288669-46tkedw7	257	36	.	.	.
cord-288669-46tkedw7	258	1	However	however	RB
cord-288669-46tkedw7	258	2	,	,	,
cord-288669-46tkedw7	258	3	no	no	DT
cord-288669-46tkedw7	258	4	direct	direct	JJ
cord-288669-46tkedw7	258	5	fusion	fusion	NN
cord-288669-46tkedw7	258	6	of	of	IN
cord-288669-46tkedw7	258	7	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	258	8	with	with	IN
cord-288669-46tkedw7	258	9	the	the	DT
cord-288669-46tkedw7	258	10	cell	cell	NN
cord-288669-46tkedw7	258	11	membrane	membrane	NN
cord-288669-46tkedw7	258	12	,	,	,
cord-288669-46tkedw7	258	13	and	and	CC
cord-288669-46tkedw7	258	14	no	no	DT
cord-288669-46tkedw7	258	15	arterivirus	arterivirus	NN
cord-288669-46tkedw7	258	16	membrane	membrane	NN
cord-288669-46tkedw7	258	17	proteins	protein	NNS
cord-288669-46tkedw7	258	18	possessing	possess	VBG
cord-288669-46tkedw7	258	19	the	the	DT
cord-288669-46tkedw7	258	20	fusogenic	fusogenic	JJ
cord-288669-46tkedw7	258	21	property	property	NN
cord-288669-46tkedw7	258	22	have	have	VBP
cord-288669-46tkedw7	258	23	been	be	VBN
cord-288669-46tkedw7	258	24	reported	report	VBN
cord-288669-46tkedw7	258	25	.	.	.
cord-288669-46tkedw7	259	1	It	-PRON-	PRP
cord-288669-46tkedw7	259	2	is	be	VBZ
cord-288669-46tkedw7	259	3	therefore	therefore	RB
cord-288669-46tkedw7	259	4	tempting	tempt	VBG
cord-288669-46tkedw7	259	5	to	to	TO
cord-288669-46tkedw7	259	6	speculate	speculate	VB
cord-288669-46tkedw7	259	7	that	that	IN
cord-288669-46tkedw7	259	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	259	9	may	may	MD
cord-288669-46tkedw7	259	10	contain	contain	VB
cord-288669-46tkedw7	259	11	a	a	DT
cord-288669-46tkedw7	259	12	viral	viral	JJ
cord-288669-46tkedw7	259	13	ion	ion	NN
cord-288669-46tkedw7	259	14	channel	channel	NN
cord-288669-46tkedw7	259	15	protein	protein	NN
cord-288669-46tkedw7	259	16	to	to	TO
cord-288669-46tkedw7	259	17	promote	promote	VB
cord-288669-46tkedw7	259	18	the	the	DT
cord-288669-46tkedw7	259	19	uncoating	uncoating	NN
cord-288669-46tkedw7	259	20	process	process	NN
cord-288669-46tkedw7	259	21	in	in	IN
cord-288669-46tkedw7	259	22	the	the	DT
cord-288669-46tkedw7	259	23	endosome	endosome	NN
cord-288669-46tkedw7	259	24	during	during	IN
cord-288669-46tkedw7	259	25	the	the	DT
cord-288669-46tkedw7	259	26	early	early	JJ
cord-288669-46tkedw7	259	27	stage	stage	NN
cord-288669-46tkedw7	259	28	of	of	IN
cord-288669-46tkedw7	259	29	infection	infection	NN
cord-288669-46tkedw7	259	30	.	.	.
cord-288669-46tkedw7	260	1	In	in	IN
cord-288669-46tkedw7	260	2	the	the	DT
cord-288669-46tkedw7	260	3	present	present	JJ
cord-288669-46tkedw7	260	4	study	study	NN
cord-288669-46tkedw7	260	5	,	,	,
cord-288669-46tkedw7	260	6	we	-PRON-	PRP
cord-288669-46tkedw7	260	7	have	have	VBP
cord-288669-46tkedw7	260	8	shown	show	VBN
cord-288669-46tkedw7	260	9	the	the	DT
cord-288669-46tkedw7	260	10	inhibitory	inhibitory	JJ
cord-288669-46tkedw7	260	11	effect	effect	NN
cord-288669-46tkedw7	260	12	of	of	IN
cord-288669-46tkedw7	260	13	ion	ion	NN
cord-288669-46tkedw7	260	14	channel	channel	NN
cord-288669-46tkedw7	260	15	blockers	blocker	NNS
cord-288669-46tkedw7	260	16	on	on	IN
cord-288669-46tkedw7	260	17	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	260	18	replication	replication	NN
cord-288669-46tkedw7	260	19	,	,	,
cord-288669-46tkedw7	260	20	indicating	indicate	VBG
cord-288669-46tkedw7	260	21	that	that	IN
cord-288669-46tkedw7	260	22	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	260	23	may	may	MD
cord-288669-46tkedw7	260	24	indeed	indeed	RB
cord-288669-46tkedw7	260	25	possess	possess	VB
cord-288669-46tkedw7	260	26	virus	virus	NN
cord-288669-46tkedw7	260	27	-	-	HYPH
cord-288669-46tkedw7	260	28	coded	code	VBN
cord-288669-46tkedw7	260	29	ion	ion	NN
cord-288669-46tkedw7	260	30	channels	channel	NNS
cord-288669-46tkedw7	260	31	whose	whose	WP$
cord-288669-46tkedw7	260	32	activity	activity	NN
cord-288669-46tkedw7	260	33	is	be	VBZ
cord-288669-46tkedw7	260	34	essential	essential	JJ
cord-288669-46tkedw7	260	35	for	for	IN
cord-288669-46tkedw7	260	36	proper	proper	JJ
cord-288669-46tkedw7	260	37	uncoating	uncoating	NN
cord-288669-46tkedw7	260	38	for	for	IN
cord-288669-46tkedw7	260	39	infection	infection	NN
cord-288669-46tkedw7	260	40	.	.	.
cord-288669-46tkedw7	261	1	Virus	virus	NN
cord-288669-46tkedw7	261	2	-	-	HYPH
cord-288669-46tkedw7	261	3	coded	code	VBN
cord-288669-46tkedw7	261	4	ion	ion	NN
cord-288669-46tkedw7	261	5	channels	channel	NNS
cord-288669-46tkedw7	261	6	,	,	,
cord-288669-46tkedw7	261	7	or	or	CC
cord-288669-46tkedw7	261	8	viroporins	viroporin	NNS
cord-288669-46tkedw7	261	9	,	,	,
cord-288669-46tkedw7	261	10	consist	consist	VBP
cord-288669-46tkedw7	261	11	of	of	IN
cord-288669-46tkedw7	261	12	small	small	JJ
cord-288669-46tkedw7	261	13	,	,	,
cord-288669-46tkedw7	261	14	highly	highly	RB
cord-288669-46tkedw7	261	15	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	261	16	proteins	protein	NNS
cord-288669-46tkedw7	261	17	generally	generally	RB
cord-288669-46tkedw7	261	18	composed	compose	VBN
cord-288669-46tkedw7	261	19	of	of	IN
cord-288669-46tkedw7	261	20	60	60	CD
cord-288669-46tkedw7	261	21	-	-	SYM
cord-288669-46tkedw7	261	22	120	120	CD
cord-288669-46tkedw7	261	23	amino	amino	NN
cord-288669-46tkedw7	261	24	acids	acid	NNS
cord-288669-46tkedw7	261	25	.	.	.
cord-288669-46tkedw7	262	1	The	the	DT
cord-288669-46tkedw7	262	2	insertion	insertion	NN
cord-288669-46tkedw7	262	3	of	of	IN
cord-288669-46tkedw7	262	4	these	these	DT
cord-288669-46tkedw7	262	5	proteins	protein	NNS
cord-288669-46tkedw7	262	6	into	into	IN
cord-288669-46tkedw7	262	7	membranes	membrane	NNS
cord-288669-46tkedw7	262	8	creates	create	VBZ
cord-288669-46tkedw7	262	9	typical	typical	JJ
cord-288669-46tkedw7	262	10	hydrophilic	hydrophilic	JJ
cord-288669-46tkedw7	262	11	pores	pore	NNS
cord-288669-46tkedw7	262	12	or	or	CC
cord-288669-46tkedw7	262	13	selective	selective	JJ
cord-288669-46tkedw7	262	14	ion	ion	NN
cord-288669-46tkedw7	262	15	channels	channel	NNS
cord-288669-46tkedw7	262	16	with	with	IN
cord-288669-46tkedw7	262	17	(	(	-LRB-
cord-288669-46tkedw7	262	18	a	a	DT
cord-288669-46tkedw7	262	19	)	)	-RRB-
cord-288669-46tkedw7	262	20	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	262	21	transmembrane	transmembrane	NN
cord-288669-46tkedw7	262	22	domain(s	domain(s	NNP
cord-288669-46tkedw7	262	23	)	)	-RRB-
cord-288669-46tkedw7	262	24	facing	face	VBG
cord-288669-46tkedw7	262	25	the	the	DT
cord-288669-46tkedw7	262	26	lipid	lipid	NN
cord-288669-46tkedw7	262	27	bilayer	bilayer	NN
cord-288669-46tkedw7	262	28	,	,	,
cord-288669-46tkedw7	262	29	leading	lead	VBG
cord-288669-46tkedw7	262	30	to	to	IN
cord-288669-46tkedw7	262	31	an	an	DT
cord-288669-46tkedw7	262	32	alteration	alteration	NN
cord-288669-46tkedw7	262	33	of	of	IN
cord-288669-46tkedw7	262	34	membrane	membrane	NN
cord-288669-46tkedw7	262	35	permeability	permeability	NN
cord-288669-46tkedw7	262	36	to	to	IN
cord-288669-46tkedw7	262	37	ions	ion	NNS
cord-288669-46tkedw7	262	38	or	or	CC
cord-288669-46tkedw7	262	39	other	other	JJ
cord-288669-46tkedw7	262	40	small	small	JJ
cord-288669-46tkedw7	262	41	molecules	molecule	NNS
cord-288669-46tkedw7	262	42	(	(	-LRB-
cord-288669-46tkedw7	262	43	for	for	IN
cord-288669-46tkedw7	262	44	a	a	DT
cord-288669-46tkedw7	262	45	review	review	NN
cord-288669-46tkedw7	262	46	,	,	,
cord-288669-46tkedw7	262	47	see	see	VB
cord-288669-46tkedw7	262	48	Fischer	Fischer	NNP
cord-288669-46tkedw7	262	49	and	and	CC
cord-288669-46tkedw7	262	50	Sansom	Sansom	NNP
cord-288669-46tkedw7	262	51	,	,	,
cord-288669-46tkedw7	262	52	2002	2002	CD
cord-288669-46tkedw7	262	53	;	;	:
cord-288669-46tkedw7	262	54	Gonzalez	Gonzalez	NNP
cord-288669-46tkedw7	262	55	and	and	CC
cord-288669-46tkedw7	262	56	Carrasco	Carrasco	NNP
cord-288669-46tkedw7	262	57	,	,	,
cord-288669-46tkedw7	262	58	2003	2003	CD
cord-288669-46tkedw7	262	59	)	)	-RRB-
cord-288669-46tkedw7	262	60	.	.	.
cord-288669-46tkedw7	263	1	Expression	expression	NN
cord-288669-46tkedw7	263	2	of	of	IN
cord-288669-46tkedw7	263	3	these	these	DT
cord-288669-46tkedw7	263	4	membrane	membrane	NN
cord-288669-46tkedw7	263	5	-	-	HYPH
cord-288669-46tkedw7	263	6	active	active	JJ
cord-288669-46tkedw7	263	7	proteins	protein	NNS
cord-288669-46tkedw7	263	8	may	may	MD
cord-288669-46tkedw7	263	9	cause	cause	VB
cord-288669-46tkedw7	263	10	cellular	cellular	JJ
cord-288669-46tkedw7	263	11	membrane	membrane	NN
cord-288669-46tkedw7	263	12	leakiness	leakiness	NN
cord-288669-46tkedw7	263	13	further	further	RB
cord-288669-46tkedw7	263	14	resulting	result	VBG
cord-288669-46tkedw7	263	15	in	in	IN
cord-288669-46tkedw7	263	16	the	the	DT
cord-288669-46tkedw7	263	17	development	development	NN
cord-288669-46tkedw7	263	18	of	of	IN
cord-288669-46tkedw7	263	19	cytopathic	cytopathic	JJ
cord-288669-46tkedw7	263	20	changes	change	NNS
cord-288669-46tkedw7	263	21	to	to	TO
cord-288669-46tkedw7	263	22	facilitate	facilitate	VB
cord-288669-46tkedw7	263	23	particle	particle	NN
cord-288669-46tkedw7	263	24	release	release	NN
cord-288669-46tkedw7	263	25	late	late	RB
cord-288669-46tkedw7	263	26	in	in	IN
cord-288669-46tkedw7	263	27	infection	infection	NN
cord-288669-46tkedw7	263	28	,	,	,
cord-288669-46tkedw7	263	29	or	or	CC
cord-288669-46tkedw7	263	30	may	may	MD
cord-288669-46tkedw7	263	31	be	be	VB
cord-288669-46tkedw7	263	32	required	require	VBN
cord-288669-46tkedw7	263	33	to	to	TO
cord-288669-46tkedw7	263	34	promote	promote	VB
cord-288669-46tkedw7	263	35	virus	virus	NN
cord-288669-46tkedw7	263	36	uncoating	uncoate	VBG
cord-288669-46tkedw7	263	37	at	at	IN
cord-288669-46tkedw7	263	38	an	an	DT
cord-288669-46tkedw7	263	39	early	early	JJ
cord-288669-46tkedw7	263	40	stage	stage	NN
cord-288669-46tkedw7	263	41	of	of	IN
cord-288669-46tkedw7	263	42	infection	infection	NN
cord-288669-46tkedw7	263	43	(	(	-LRB-
cord-288669-46tkedw7	263	44	reviewed	review	VBN
cord-288669-46tkedw7	263	45	by	by	IN
cord-288669-46tkedw7	263	46	Carrasco	Carrasco	NNP
cord-288669-46tkedw7	263	47	,	,	,
cord-288669-46tkedw7	263	48	1995	1995	CD
cord-288669-46tkedw7	263	49	)	)	-RRB-
cord-288669-46tkedw7	263	50	.	.	.
cord-288669-46tkedw7	264	1	The	the	DT
cord-288669-46tkedw7	264	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	264	3	E	E	NNP
cord-288669-46tkedw7	264	4	protein	protein	NN
cord-288669-46tkedw7	264	5	structurally	structurally	RB
cord-288669-46tkedw7	264	6	resembles	resemble	VBZ
cord-288669-46tkedw7	264	7	a	a	DT
cord-288669-46tkedw7	264	8	number	number	NN
cord-288669-46tkedw7	264	9	of	of	IN
cord-288669-46tkedw7	264	10	viroporins	viroporin	NNS
cord-288669-46tkedw7	264	11	found	find	VBN
cord-288669-46tkedw7	264	12	in	in	IN
cord-288669-46tkedw7	264	13	other	other	JJ
cord-288669-46tkedw7	264	14	RNA	RNA	NNP
cord-288669-46tkedw7	264	15	viruses	virus	NNS
cord-288669-46tkedw7	264	16	,	,	,
cord-288669-46tkedw7	264	17	in	in	IN
cord-288669-46tkedw7	264	18	that	that	IN
cord-288669-46tkedw7	264	19	it	-PRON-	PRP
cord-288669-46tkedw7	264	20	consists	consist	VBZ
cord-288669-46tkedw7	264	21	of	of	IN
cord-288669-46tkedw7	264	22	70	70	CD
cord-288669-46tkedw7	264	23	-	-	SYM
cord-288669-46tkedw7	264	24	73	73	CD
cord-288669-46tkedw7	264	25	amino	amino	NN
cord-288669-46tkedw7	264	26	acid	acid	NN
cord-288669-46tkedw7	264	27	residues	residue	NNS
cord-288669-46tkedw7	264	28	and	and	CC
cord-288669-46tkedw7	264	29	contains	contain	VBZ
cord-288669-46tkedw7	264	30	central	central	JJ
cord-288669-46tkedw7	264	31	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	264	32	sequences	sequence	NNS
cord-288669-46tkedw7	264	33	with	with	IN
cord-288669-46tkedw7	264	34	a	a	DT
cord-288669-46tkedw7	264	35	cluster	cluster	NN
cord-288669-46tkedw7	264	36	of	of	IN
cord-288669-46tkedw7	264	37	basic	basic	JJ
cord-288669-46tkedw7	264	38	amino	amino	NN
cord-288669-46tkedw7	264	39	acids	acid	NNS
cord-288669-46tkedw7	264	40	at	at	IN
cord-288669-46tkedw7	264	41	the	the	DT
cord-288669-46tkedw7	264	42	Cterminus	Cterminus	NNP
cord-288669-46tkedw7	264	43	.	.	.
cord-288669-46tkedw7	265	1	The	the	DT
cord-288669-46tkedw7	265	2	E	e	NN
cord-288669-46tkedw7	265	3	protein	protein	NN
cord-288669-46tkedw7	265	4	localizes	localize	VBZ
cord-288669-46tkedw7	265	5	mainly	mainly	RB
cord-288669-46tkedw7	265	6	in	in	IN
cord-288669-46tkedw7	265	7	perinuclear	perinuclear	NN
cord-288669-46tkedw7	265	8	regions	region	NNS
cord-288669-46tkedw7	265	9	including	include	VBG
cord-288669-46tkedw7	265	10	the	the	DT
cord-288669-46tkedw7	265	11	ER	ER	NNP
cord-288669-46tkedw7	265	12	and	and	CC
cord-288669-46tkedw7	265	13	the	the	DT
cord-288669-46tkedw7	265	14	Golgi	Golgi	NNP
cord-288669-46tkedw7	265	15	complex	complex	NN
cord-288669-46tkedw7	265	16	and	and	CC
cord-288669-46tkedw7	265	17	associates	associate	NNS
cord-288669-46tkedw7	265	18	with	with	IN
cord-288669-46tkedw7	265	19	intracellular	intracellular	JJ
cord-288669-46tkedw7	265	20	membranes	membrane	NNS
cord-288669-46tkedw7	265	21	(	(	-LRB-
cord-288669-46tkedw7	265	22	Wu	Wu	NNP
cord-288669-46tkedw7	265	23	et	et	NNP
cord-288669-46tkedw7	265	24	al	al	NNP
cord-288669-46tkedw7	265	25	.	.	NNP
cord-288669-46tkedw7	265	26	,	,	,
cord-288669-46tkedw7	265	27	2001	2001	CD
cord-288669-46tkedw7	265	28	)	)	-RRB-
cord-288669-46tkedw7	265	29	.	.	.
cord-288669-46tkedw7	266	1	Interestingly	interestingly	RB
cord-288669-46tkedw7	266	2	,	,	,
cord-288669-46tkedw7	266	3	coronaviruses	coronaviruse	NNS
cord-288669-46tkedw7	266	4	,	,	,
cord-288669-46tkedw7	266	5	another	another	DT
cord-288669-46tkedw7	266	6	family	family	NN
cord-288669-46tkedw7	266	7	member	member	NN
cord-288669-46tkedw7	266	8	of	of	IN
cord-288669-46tkedw7	266	9	the	the	DT
cord-288669-46tkedw7	266	10	order	order	NN
cord-288669-46tkedw7	266	11	Nidovirales	Nidovirales	NNP
cord-288669-46tkedw7	266	12	,	,	,
cord-288669-46tkedw7	266	13	also	also	RB
cord-288669-46tkedw7	266	14	code	code	NN
cord-288669-46tkedw7	266	15	for	for	IN
cord-288669-46tkedw7	266	16	a	a	DT
cord-288669-46tkedw7	266	17	small	small	JJ
cord-288669-46tkedw7	266	18	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	266	19	membrane	membrane	NN
cord-288669-46tkedw7	266	20	,	,	,
cord-288669-46tkedw7	266	21	designated	designate	VBN
cord-288669-46tkedw7	266	22	E	e	NN
cord-288669-46tkedw7	266	23	protein	protein	NN
cord-288669-46tkedw7	266	24	,	,	,
cord-288669-46tkedw7	266	25	which	which	WDT
cord-288669-46tkedw7	266	26	may	may	MD
cord-288669-46tkedw7	266	27	play	play	VB
cord-288669-46tkedw7	266	28	a	a	DT
cord-288669-46tkedw7	266	29	crucial	crucial	JJ
cord-288669-46tkedw7	266	30	role	role	NN
cord-288669-46tkedw7	266	31	during	during	IN
cord-288669-46tkedw7	266	32	virus	virus	NN
cord-288669-46tkedw7	266	33	morphogenesis	morphogenesis	NN
cord-288669-46tkedw7	266	34	(	(	-LRB-
cord-288669-46tkedw7	266	35	Fischer	Fischer	NNP
cord-288669-46tkedw7	266	36	et	et	NNP
cord-288669-46tkedw7	266	37	al	al	NNP
cord-288669-46tkedw7	266	38	.	.	NNP
cord-288669-46tkedw7	266	39	,	,	,
cord-288669-46tkedw7	266	40	1998	1998	CD
cord-288669-46tkedw7	266	41	;	;	:
cord-288669-46tkedw7	266	42	Liu	Liu	NNP
cord-288669-46tkedw7	266	43	and	and	CC
cord-288669-46tkedw7	266	44	Inglis	Inglis	NNP
cord-288669-46tkedw7	266	45	,	,	,
cord-288669-46tkedw7	266	46	1991	1991	CD
cord-288669-46tkedw7	266	47	)	)	-RRB-
cord-288669-46tkedw7	266	48	.	.	.
cord-288669-46tkedw7	267	1	In	in	IN
cord-288669-46tkedw7	267	2	recent	recent	JJ
cord-288669-46tkedw7	267	3	studies	study	NNS
cord-288669-46tkedw7	267	4	,	,	,
cord-288669-46tkedw7	267	5	the	the	DT
cord-288669-46tkedw7	267	6	coronavirus	coronavirus	NN
cord-288669-46tkedw7	267	7	E	e	NN
cord-288669-46tkedw7	267	8	protein	protein	NN
cord-288669-46tkedw7	267	9	has	have	VBZ
cord-288669-46tkedw7	267	10	been	be	VBN
cord-288669-46tkedw7	267	11	shown	show	VBN
cord-288669-46tkedw7	267	12	to	to	TO
cord-288669-46tkedw7	267	13	modify	modify	VB
cord-288669-46tkedw7	267	14	membrane	membrane	NN
cord-288669-46tkedw7	267	15	permeability	permeability	NN
cord-288669-46tkedw7	267	16	(	(	-LRB-
cord-288669-46tkedw7	267	17	Liao	Liao	NNP
cord-288669-46tkedw7	267	18	et	et	FW
cord-288669-46tkedw7	267	19	al	al	NNP
cord-288669-46tkedw7	267	20	.	.	NNP
cord-288669-46tkedw7	267	21	,	,	,
cord-288669-46tkedw7	267	22	2004	2004	CD
cord-288669-46tkedw7	267	23	;	;	:
cord-288669-46tkedw7	267	24	Madan	Madan	NNP
cord-288669-46tkedw7	267	25	et	et	NNP
cord-288669-46tkedw7	267	26	al	al	NNP
cord-288669-46tkedw7	267	27	.	.	NNP
cord-288669-46tkedw7	267	28	,	,	,
cord-288669-46tkedw7	267	29	2005	2005	CD
cord-288669-46tkedw7	267	30	)	)	-RRB-
cord-288669-46tkedw7	267	31	as	as	RB
cord-288669-46tkedw7	267	32	well	well	RB
cord-288669-46tkedw7	267	33	as	as	IN
cord-288669-46tkedw7	267	34	to	to	TO
cord-288669-46tkedw7	267	35	form	form	VB
cord-288669-46tkedw7	267	36	cation	cation	NN
cord-288669-46tkedw7	267	37	-	-	HYPH
cord-288669-46tkedw7	267	38	selective	selective	JJ
cord-288669-46tkedw7	267	39	ion	ion	NN
cord-288669-46tkedw7	267	40	channels	channel	NNS
cord-288669-46tkedw7	267	41	in	in	IN
cord-288669-46tkedw7	267	42	an	an	DT
cord-288669-46tkedw7	267	43	artificial	artificial	JJ
cord-288669-46tkedw7	267	44	membrane	membrane	NN
cord-288669-46tkedw7	267	45	(	(	-LRB-
cord-288669-46tkedw7	267	46	Wilson	Wilson	NNP
cord-288669-46tkedw7	267	47	et	et	NNP
cord-288669-46tkedw7	267	48	al	al	NNP
cord-288669-46tkedw7	267	49	.	.	NNP
cord-288669-46tkedw7	267	50	,	,	,
cord-288669-46tkedw7	267	51	2004	2004	CD
cord-288669-46tkedw7	267	52	)	)	-RRB-
cord-288669-46tkedw7	267	53	.	.	.
cord-288669-46tkedw7	268	1	Our	-PRON-	PRP$
cord-288669-46tkedw7	268	2	data	datum	NNS
cord-288669-46tkedw7	268	3	in	in	IN
cord-288669-46tkedw7	268	4	the	the	DT
cord-288669-46tkedw7	268	5	current	current	JJ
cord-288669-46tkedw7	268	6	study	study	NN
cord-288669-46tkedw7	268	7	also	also	RB
cord-288669-46tkedw7	268	8	demonstrate	demonstrate	VBP
cord-288669-46tkedw7	268	9	the	the	DT
cord-288669-46tkedw7	268	10	alteration	alteration	NN
cord-288669-46tkedw7	268	11	of	of	IN
cord-288669-46tkedw7	268	12	membrane	membrane	NN
cord-288669-46tkedw7	268	13	permeability	permeability	NN
cord-288669-46tkedw7	268	14	by	by	IN
cord-288669-46tkedw7	268	15	the	the	DT
cord-288669-46tkedw7	268	16	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	268	17	E	E	NNP
cord-288669-46tkedw7	268	18	protein	protein	NN
cord-288669-46tkedw7	268	19	and	and	CC
cord-288669-46tkedw7	268	20	the	the	DT
cord-288669-46tkedw7	268	21	inhibition	inhibition	NN
cord-288669-46tkedw7	268	22	of	of	IN
cord-288669-46tkedw7	268	23	bacterial	bacterial	JJ
cord-288669-46tkedw7	268	24	growth	growth	NN
cord-288669-46tkedw7	268	25	by	by	IN
cord-288669-46tkedw7	268	26	the	the	DT
cord-288669-46tkedw7	268	27	increase	increase	NN
cord-288669-46tkedw7	268	28	of	of	IN
cord-288669-46tkedw7	268	29	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	268	30	B	b	NN
cord-288669-46tkedw7	268	31	penetration	penetration	NN
cord-288669-46tkedw7	268	32	into	into	IN
cord-288669-46tkedw7	268	33	bacterial	bacterial	JJ
cord-288669-46tkedw7	268	34	cells	cell	NNS
cord-288669-46tkedw7	268	35	.	.	.
cord-288669-46tkedw7	269	1	However	however	RB
cord-288669-46tkedw7	269	2	,	,	,
cord-288669-46tkedw7	269	3	we	-PRON-	PRP
cord-288669-46tkedw7	269	4	were	be	VBD
cord-288669-46tkedw7	269	5	not	not	RB
cord-288669-46tkedw7	269	6	able	able	JJ
cord-288669-46tkedw7	269	7	to	to	TO
cord-288669-46tkedw7	269	8	show	show	VB
cord-288669-46tkedw7	269	9	similar	similar	JJ
cord-288669-46tkedw7	269	10	results	result	NNS
cord-288669-46tkedw7	269	11	in	in	IN
cord-288669-46tkedw7	269	12	mammalian	mammalian	JJ
cord-288669-46tkedw7	269	13	cells	cell	NNS
cord-288669-46tkedw7	269	14	by	by	IN
cord-288669-46tkedw7	269	15	infection	infection	NN
cord-288669-46tkedw7	269	16	or	or	CC
cord-288669-46tkedw7	269	17	E	e	NN
cord-288669-46tkedw7	269	18	gene	gene	NN
cord-288669-46tkedw7	269	19	transfection	transfection	NN
cord-288669-46tkedw7	269	20	,	,	,
cord-288669-46tkedw7	269	21	which	which	WDT
cord-288669-46tkedw7	269	22	may	may	MD
cord-288669-46tkedw7	269	23	be	be	VB
cord-288669-46tkedw7	269	24	due	due	JJ
cord-288669-46tkedw7	269	25	to	to	IN
cord-288669-46tkedw7	269	26	the	the	DT
cord-288669-46tkedw7	269	27	different	different	JJ
cord-288669-46tkedw7	269	28	intracellular	intracellular	JJ
cord-288669-46tkedw7	269	29	localization	localization	NN
cord-288669-46tkedw7	269	30	of	of	IN
cord-288669-46tkedw7	269	31	E	e	NN
cord-288669-46tkedw7	269	32	in	in	IN
cord-288669-46tkedw7	269	33	mammalian	mammalian	JJ
cord-288669-46tkedw7	269	34	cells	cell	NNS
cord-288669-46tkedw7	269	35	.	.	.
cord-288669-46tkedw7	270	1	In	in	IN
cord-288669-46tkedw7	270	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	270	3	-	-	HYPH
cord-288669-46tkedw7	270	4	infected	infect	VBN
cord-288669-46tkedw7	270	5	cells	cell	NNS
cord-288669-46tkedw7	270	6	,	,	,
cord-288669-46tkedw7	270	7	the	the	DT
cord-288669-46tkedw7	270	8	E	e	NN
cord-288669-46tkedw7	270	9	protein	protein	NN
cord-288669-46tkedw7	270	10	appears	appear	VBZ
cord-288669-46tkedw7	270	11	to	to	TO
cord-288669-46tkedw7	270	12	remain	remain	VB
cord-288669-46tkedw7	270	13	in	in	IN
cord-288669-46tkedw7	270	14	the	the	DT
cord-288669-46tkedw7	270	15	ER	ER	NNP
cord-288669-46tkedw7	270	16	and	and	CC
cord-288669-46tkedw7	270	17	Golgi	Golgi	NNP
cord-288669-46tkedw7	270	18	complex	complex	NN
cord-288669-46tkedw7	270	19	,	,	,
cord-288669-46tkedw7	270	20	where	where	WRB
cord-288669-46tkedw7	270	21	it	-PRON-	PRP
cord-288669-46tkedw7	270	22	likely	likely	RB
cord-288669-46tkedw7	270	23	participates	participate	VBZ
cord-288669-46tkedw7	270	24	in	in	IN
cord-288669-46tkedw7	270	25	assembly	assembly	NN
cord-288669-46tkedw7	270	26	of	of	IN
cord-288669-46tkedw7	270	27	infectious	infectious	JJ
cord-288669-46tkedw7	270	28	progeny	progeny	JJ
cord-288669-46tkedw7	270	29	virus	virus	NN
cord-288669-46tkedw7	270	30	,	,	,
cord-288669-46tkedw7	270	31	rather	rather	RB
cord-288669-46tkedw7	270	32	than	than	IN
cord-288669-46tkedw7	270	33	traveling	travel	VBG
cord-288669-46tkedw7	270	34	to	to	IN
cord-288669-46tkedw7	270	35	the	the	DT
cord-288669-46tkedw7	270	36	plasma	plasma	NN
cord-288669-46tkedw7	270	37	membrane	membrane	NN
cord-288669-46tkedw7	270	38	.	.	.
cord-288669-46tkedw7	271	1	Bacterial	bacterial	JJ
cord-288669-46tkedw7	271	2	cells	cell	NNS
cord-288669-46tkedw7	271	3	do	do	VBP
cord-288669-46tkedw7	271	4	not	not	RB
cord-288669-46tkedw7	271	5	possess	possess	VB
cord-288669-46tkedw7	271	6	such	such	JJ
cord-288669-46tkedw7	271	7	intracellular	intracellular	JJ
cord-288669-46tkedw7	271	8	organelles	organelle	NNS
cord-288669-46tkedw7	271	9	and	and	CC
cord-288669-46tkedw7	271	10	the	the	DT
cord-288669-46tkedw7	271	11	expressed	express	VBN
cord-288669-46tkedw7	271	12	E	e	NN
cord-288669-46tkedw7	271	13	protein	protein	NN
cord-288669-46tkedw7	271	14	may	may	MD
cord-288669-46tkedw7	271	15	accumulate	accumulate	VB
cord-288669-46tkedw7	271	16	at	at	IN
cord-288669-46tkedw7	271	17	the	the	DT
cord-288669-46tkedw7	271	18	inner	inner	JJ
cord-288669-46tkedw7	271	19	bacterial	bacterial	JJ
cord-288669-46tkedw7	271	20	membrane	membrane	NN
cord-288669-46tkedw7	271	21	,	,	,
cord-288669-46tkedw7	271	22	leading	lead	VBG
cord-288669-46tkedw7	271	23	to	to	IN
cord-288669-46tkedw7	271	24	membrane	membrane	NN
cord-288669-46tkedw7	271	25	perturbation	perturbation	NN
cord-288669-46tkedw7	271	26	,	,	,
cord-288669-46tkedw7	271	27	and	and	CC
cord-288669-46tkedw7	271	28	thus	thus	RB
cord-288669-46tkedw7	271	29	enhancing	enhance	VBG
cord-288669-46tkedw7	271	30	membrane	membrane	NN
cord-288669-46tkedw7	271	31	permeabilization	permeabilization	NN
cord-288669-46tkedw7	271	32	.	.	.
cord-288669-46tkedw7	272	1	Failure	failure	NN
cord-288669-46tkedw7	272	2	to	to	TO
cord-288669-46tkedw7	272	3	observe	observe	VB
cord-288669-46tkedw7	272	4	the	the	DT
cord-288669-46tkedw7	272	5	direct	direct	JJ
cord-288669-46tkedw7	272	6	alteration	alteration	NN
cord-288669-46tkedw7	272	7	of	of	IN
cord-288669-46tkedw7	272	8	membrane	membrane	NN
cord-288669-46tkedw7	272	9	permeability	permeability	NN
cord-288669-46tkedw7	272	10	by	by	IN
cord-288669-46tkedw7	272	11	E	E	NNS
cord-288669-46tkedw7	272	12	in	in	IN
cord-288669-46tkedw7	272	13	mammalian	mammalian	JJ
cord-288669-46tkedw7	272	14	cells	cell	NNS
cord-288669-46tkedw7	272	15	implies	imply	VBZ
cord-288669-46tkedw7	272	16	that	that	IN
cord-288669-46tkedw7	272	17	the	the	DT
cord-288669-46tkedw7	272	18	role	role	NN
cord-288669-46tkedw7	272	19	of	of	IN
cord-288669-46tkedw7	272	20	E	E	NNP
cord-288669-46tkedw7	272	21	is	be	VBZ
cord-288669-46tkedw7	272	22	not	not	RB
cord-288669-46tkedw7	272	23	linked	link	VBN
cord-288669-46tkedw7	272	24	to	to	IN
cord-288669-46tkedw7	272	25	membrane	membrane	NN
cord-288669-46tkedw7	272	26	disorganization	disorganization	NN
cord-288669-46tkedw7	272	27	and	and	CC
cord-288669-46tkedw7	272	28	cell	cell	NN
cord-288669-46tkedw7	272	29	lysis	lysis	NN
cord-288669-46tkedw7	272	30	in	in	IN
cord-288669-46tkedw7	272	31	facilitating	facilitate	VBG
cord-288669-46tkedw7	272	32	virus	virus	NN
cord-288669-46tkedw7	272	33	release	release	NN
cord-288669-46tkedw7	272	34	.	.	.
cord-288669-46tkedw7	273	1	The	the	DT
cord-288669-46tkedw7	273	2	cross	cross	NN
cord-288669-46tkedw7	273	3	-	-	JJ
cord-288669-46tkedw7	273	4	linking	linking	JJ
cord-288669-46tkedw7	273	5	studies	study	NNS
cord-288669-46tkedw7	273	6	show	show	VBP
cord-288669-46tkedw7	273	7	that	that	IN
cord-288669-46tkedw7	273	8	the	the	DT
cord-288669-46tkedw7	273	9	E	e	NN
cord-288669-46tkedw7	273	10	protein	protein	NN
cord-288669-46tkedw7	273	11	can	can	MD
cord-288669-46tkedw7	273	12	form	form	VB
cord-288669-46tkedw7	273	13	homo	homo	NN
cord-288669-46tkedw7	273	14	-	-	HYPH
cord-288669-46tkedw7	273	15	oligomers	oligomer	NNS
cord-288669-46tkedw7	273	16	,	,	,
cord-288669-46tkedw7	273	17	including	include	VBG
cord-288669-46tkedw7	273	18	dimers	dimer	NNS
cord-288669-46tkedw7	273	19	,	,	,
cord-288669-46tkedw7	273	20	trimers	trimer	NNS
cord-288669-46tkedw7	273	21	and	and	CC
cord-288669-46tkedw7	273	22	tetramers	tetramer	NNS
cord-288669-46tkedw7	273	23	by	by	IN
cord-288669-46tkedw7	273	24	non	non	JJ
cord-288669-46tkedw7	273	25	-	-	JJ
cord-288669-46tkedw7	273	26	covalent	covalent	JJ
cord-288669-46tkedw7	273	27	interactions	interaction	NNS
cord-288669-46tkedw7	273	28	.	.	.
cord-288669-46tkedw7	274	1	All	all	DT
cord-288669-46tkedw7	274	2	information	information	NN
cord-288669-46tkedw7	274	3	obtained	obtain	VBN
cord-288669-46tkedw7	274	4	in	in	IN
cord-288669-46tkedw7	274	5	the	the	DT
cord-288669-46tkedw7	274	6	present	present	JJ
cord-288669-46tkedw7	274	7	study	study	NN
cord-288669-46tkedw7	274	8	support	support	VBP
cord-288669-46tkedw7	274	9	the	the	DT
cord-288669-46tkedw7	274	10	hypothesis	hypothesis	NN
cord-288669-46tkedw7	274	11	that	that	IN
cord-288669-46tkedw7	274	12	the	the	DT
cord-288669-46tkedw7	274	13	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	274	14	E	E	NNP
cord-288669-46tkedw7	274	15	protein	protein	NN
cord-288669-46tkedw7	274	16	contains	contain	VBZ
cord-288669-46tkedw7	274	17	a	a	DT
cord-288669-46tkedw7	274	18	potential	potential	NN
cord-288669-46tkedw7	274	19	for	for	IN
cord-288669-46tkedw7	274	20	a	a	DT
cord-288669-46tkedw7	274	21	pore	pore	NN
cord-288669-46tkedw7	274	22	-	-	HYPH
cord-288669-46tkedw7	274	23	forming	form	VBG
cord-288669-46tkedw7	274	24	activity	activity	NN
cord-288669-46tkedw7	274	25	and	and	CC
cord-288669-46tkedw7	274	26	thus	thus	RB
cord-288669-46tkedw7	274	27	may	may	MD
cord-288669-46tkedw7	274	28	function	function	VB
cord-288669-46tkedw7	274	29	as	as	IN
cord-288669-46tkedw7	274	30	an	an	DT
cord-288669-46tkedw7	274	31	ion	ion	NN
cord-288669-46tkedw7	274	32	channel	channel	NN
cord-288669-46tkedw7	274	33	for	for	IN
cord-288669-46tkedw7	274	34	virus	virus	NN
cord-288669-46tkedw7	274	35	uncoating	uncoating	NN
cord-288669-46tkedw7	274	36	.	.	.
cord-288669-46tkedw7	275	1	The	the	DT
cord-288669-46tkedw7	275	2	functional	functional	JJ
cord-288669-46tkedw7	275	3	and	and	CC
cord-288669-46tkedw7	275	4	structural	structural	JJ
cord-288669-46tkedw7	275	5	features	feature	NNS
cord-288669-46tkedw7	275	6	of	of	IN
cord-288669-46tkedw7	275	7	the	the	DT
cord-288669-46tkedw7	275	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	275	9	E	E	NNP
cord-288669-46tkedw7	275	10	protein	protein	NN
cord-288669-46tkedw7	275	11	resemble	resemble	VBP
cord-288669-46tkedw7	275	12	the	the	DT
cord-288669-46tkedw7	275	13	influenza	influenza	NN
cord-288669-46tkedw7	275	14	A	a	DT
cord-288669-46tkedw7	275	15	virus	virus	NN
cord-288669-46tkedw7	275	16	M2	m2	NN
cord-288669-46tkedw7	275	17	protein	protein	NN
cord-288669-46tkedw7	275	18	that	that	WDT
cord-288669-46tkedw7	275	19	is	be	VBZ
cord-288669-46tkedw7	275	20	best	well	RBS
cord-288669-46tkedw7	275	21	characterized	characterize	VBN
cord-288669-46tkedw7	275	22	as	as	IN
cord-288669-46tkedw7	275	23	an	an	DT
cord-288669-46tkedw7	275	24	ion	ion	NN
cord-288669-46tkedw7	275	25	-	-	HYPH
cord-288669-46tkedw7	275	26	channel	channel	NN
cord-288669-46tkedw7	275	27	protein	protein	NN
cord-288669-46tkedw7	275	28	.	.	.
cord-288669-46tkedw7	276	1	The	the	DT
cord-288669-46tkedw7	276	2	M2	M2	NNP
cord-288669-46tkedw7	276	3	protein	protein	NN
cord-288669-46tkedw7	276	4	forms	form	VBZ
cord-288669-46tkedw7	276	5	a	a	DT
cord-288669-46tkedw7	276	6	homo	homo	NN
cord-288669-46tkedw7	276	7	-	-	HYPH
cord-288669-46tkedw7	276	8	tetramer	tetramer	NN
cord-288669-46tkedw7	276	9	in	in	IN
cord-288669-46tkedw7	276	10	the	the	DT
cord-288669-46tkedw7	276	11	viral	viral	JJ
cord-288669-46tkedw7	276	12	membrane	membrane	NN
cord-288669-46tkedw7	276	13	and	and	CC
cord-288669-46tkedw7	276	14	functions	function	NNS
cord-288669-46tkedw7	276	15	as	as	IN
cord-288669-46tkedw7	276	16	an	an	DT
cord-288669-46tkedw7	276	17	ion	ion	NN
cord-288669-46tkedw7	276	18	channel	channel	NN
cord-288669-46tkedw7	276	19	.	.	.
cord-288669-46tkedw7	277	1	The	the	DT
cord-288669-46tkedw7	277	2	M2	m2	JJ
cord-288669-46tkedw7	277	3	channel	channel	NN
cord-288669-46tkedw7	277	4	allows	allow	VBZ
cord-288669-46tkedw7	277	5	the	the	DT
cord-288669-46tkedw7	277	6	translocation	translocation	NN
cord-288669-46tkedw7	277	7	of	of	IN
cord-288669-46tkedw7	277	8	protons	proton	NNS
cord-288669-46tkedw7	277	9	from	from	IN
cord-288669-46tkedw7	277	10	the	the	DT
cord-288669-46tkedw7	277	11	acidic	acidic	JJ
cord-288669-46tkedw7	277	12	environment	environment	NN
cord-288669-46tkedw7	277	13	of	of	IN
cord-288669-46tkedw7	277	14	the	the	DT
cord-288669-46tkedw7	277	15	endosome	endosome	NN
cord-288669-46tkedw7	277	16	to	to	IN
cord-288669-46tkedw7	277	17	the	the	DT
cord-288669-46tkedw7	277	18	inner	inner	JJ
cord-288669-46tkedw7	277	19	space	space	NN
cord-288669-46tkedw7	277	20	of	of	IN
cord-288669-46tkedw7	277	21	virions	virion	NNS
cord-288669-46tkedw7	277	22	and	and	CC
cord-288669-46tkedw7	277	23	alters	alter	VBZ
cord-288669-46tkedw7	277	24	the	the	DT
cord-288669-46tkedw7	277	25	pH	ph	NN
cord-288669-46tkedw7	277	26	in	in	IN
cord-288669-46tkedw7	277	27	the	the	DT
cord-288669-46tkedw7	277	28	virion	virion	NN
cord-288669-46tkedw7	277	29	.	.	.
cord-288669-46tkedw7	278	1	As	as	IN
cord-288669-46tkedw7	278	2	a	a	DT
cord-288669-46tkedw7	278	3	consequence	consequence	NN
cord-288669-46tkedw7	278	4	,	,	,
cord-288669-46tkedw7	278	5	the	the	DT
cord-288669-46tkedw7	278	6	M1	M1	NNP
cord-288669-46tkedw7	278	7	protein	protein	NN
cord-288669-46tkedw7	278	8	in	in	IN
cord-288669-46tkedw7	278	9	the	the	DT
cord-288669-46tkedw7	278	10	virion	virion	NN
cord-288669-46tkedw7	278	11	is	be	VBZ
cord-288669-46tkedw7	278	12	dissociated	dissociate	VBN
cord-288669-46tkedw7	278	13	from	from	IN
cord-288669-46tkedw7	278	14	the	the	DT
cord-288669-46tkedw7	278	15	viral	viral	JJ
cord-288669-46tkedw7	278	16	ribonucleoprotein	ribonucleoprotein	NN
cord-288669-46tkedw7	278	17	complex	complex	NN
cord-288669-46tkedw7	278	18	,	,	,
cord-288669-46tkedw7	278	19	which	which	WDT
cord-288669-46tkedw7	278	20	promotes	promote	VBZ
cord-288669-46tkedw7	278	21	the	the	DT
cord-288669-46tkedw7	278	22	ribonucleoprotein	ribonucleoprotein	NN
cord-288669-46tkedw7	278	23	complex	complex	NN
cord-288669-46tkedw7	278	24	traveling	travel	VBG
cord-288669-46tkedw7	278	25	to	to	IN
cord-288669-46tkedw7	278	26	the	the	DT
cord-288669-46tkedw7	278	27	nucleus	nucleus	NN
cord-288669-46tkedw7	278	28	where	where	WRB
cord-288669-46tkedw7	278	29	the	the	DT
cord-288669-46tkedw7	278	30	influenza	influenza	NN
cord-288669-46tkedw7	278	31	replication	replication	NN
cord-288669-46tkedw7	278	32	takes	take	VBZ
cord-288669-46tkedw7	278	33	place	place	NN
cord-288669-46tkedw7	278	34	(	(	-LRB-
cord-288669-46tkedw7	278	35	Pinto	Pinto	NNP
cord-288669-46tkedw7	278	36	et	et	NNP
cord-288669-46tkedw7	278	37	al	al	NNP
cord-288669-46tkedw7	278	38	.	.	NNP
cord-288669-46tkedw7	278	39	,	,	,
cord-288669-46tkedw7	278	40	1992	1992	CD
cord-288669-46tkedw7	278	41	;	;	:
cord-288669-46tkedw7	278	42	Sakaguchi	Sakaguchi	NNP
cord-288669-46tkedw7	278	43	et	et	NNP
cord-288669-46tkedw7	278	44	al	al	NNP
cord-288669-46tkedw7	278	45	.	.	NNP
cord-288669-46tkedw7	278	46	,	,	,
cord-288669-46tkedw7	278	47	1997	1997	CD
cord-288669-46tkedw7	278	48	)	)	-RRB-
cord-288669-46tkedw7	278	49	.	.	.
cord-288669-46tkedw7	279	1	It	-PRON-	PRP
cord-288669-46tkedw7	279	2	is	be	VBZ
cord-288669-46tkedw7	279	3	noteworthy	noteworthy	JJ
cord-288669-46tkedw7	279	4	that	that	IN
cord-288669-46tkedw7	279	5	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	279	6	replication	replication	NN
cord-288669-46tkedw7	279	7	could	could	MD
cord-288669-46tkedw7	279	8	be	be	VB
cord-288669-46tkedw7	279	9	inhibited	inhibit	VBN
cord-288669-46tkedw7	279	10	by	by	IN
cord-288669-46tkedw7	279	11	amantadine	amantadine	NNP
cord-288669-46tkedw7	279	12	,	,	,
cord-288669-46tkedw7	279	13	an	an	DT
cord-288669-46tkedw7	279	14	antiviral	antiviral	JJ
cord-288669-46tkedw7	279	15	drug	drug	NN
cord-288669-46tkedw7	279	16	for	for	IN
cord-288669-46tkedw7	279	17	influenza	influenza	NN
cord-288669-46tkedw7	279	18	virus	virus	NN
cord-288669-46tkedw7	279	19	(	(	-LRB-
cord-288669-46tkedw7	279	20	Kreutz	Kreutz	NNP
cord-288669-46tkedw7	279	21	and	and	CC
cord-288669-46tkedw7	279	22	Ackermann	Ackermann	NNP
cord-288669-46tkedw7	279	23	,	,	,
cord-288669-46tkedw7	279	24	1996	1996	CD
cord-288669-46tkedw7	279	25	;	;	:
cord-288669-46tkedw7	279	26	Fig	Fig	NNP
cord-288669-46tkedw7	279	27	.	.	NNP
cord-288669-46tkedw7	279	28	5	5	CD
cord-288669-46tkedw7	279	29	)	)	-RRB-
cord-288669-46tkedw7	279	30	.	.	.
cord-288669-46tkedw7	280	1	In	in	IN
cord-288669-46tkedw7	280	2	summary	summary	NN
cord-288669-46tkedw7	280	3	,	,	,
cord-288669-46tkedw7	280	4	we	-PRON-	PRP
cord-288669-46tkedw7	280	5	propose	propose	VBP
cord-288669-46tkedw7	280	6	a	a	DT
cord-288669-46tkedw7	280	7	model	model	NN
cord-288669-46tkedw7	280	8	for	for	IN
cord-288669-46tkedw7	280	9	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	280	10	uncoating	uncoate	VBG
cord-288669-46tkedw7	280	11	based	base	VBN
cord-288669-46tkedw7	280	12	on	on	IN
cord-288669-46tkedw7	280	13	our	-PRON-	PRP$
cord-288669-46tkedw7	280	14	findings	finding	NNS
cord-288669-46tkedw7	280	15	.	.	.
cord-288669-46tkedw7	281	1	In	in	IN
cord-288669-46tkedw7	281	2	this	this	DT
cord-288669-46tkedw7	281	3	model	model	NN
cord-288669-46tkedw7	281	4	,	,	,
cord-288669-46tkedw7	281	5	E	e	NN
cord-288669-46tkedw7	281	6	proteins	protein	NNS
cord-288669-46tkedw7	281	7	form	form	NN
cord-288669-46tkedw7	281	8	pores	pore	NNS
cord-288669-46tkedw7	281	9	(	(	-LRB-
cord-288669-46tkedw7	281	10	ion	ion	NN
cord-288669-46tkedw7	281	11	channels	channel	NNS
cord-288669-46tkedw7	281	12	)	)	-RRB-
cord-288669-46tkedw7	281	13	in	in	IN
cord-288669-46tkedw7	281	14	the	the	DT
cord-288669-46tkedw7	281	15	viral	viral	JJ
cord-288669-46tkedw7	281	16	envelope	envelope	NN
cord-288669-46tkedw7	281	17	.	.	.
cord-288669-46tkedw7	282	1	Upon	upon	IN
cord-288669-46tkedw7	282	2	internalization	internalization	NN
cord-288669-46tkedw7	282	3	by	by	IN
cord-288669-46tkedw7	282	4	receptor	receptor	NN
cord-288669-46tkedw7	282	5	-	-	HYPH
cord-288669-46tkedw7	282	6	mediated	mediate	VBN
cord-288669-46tkedw7	282	7	endocytosis	endocytosis	NN
cord-288669-46tkedw7	282	8	through	through	IN
cord-288669-46tkedw7	282	9	small	small	JJ
cord-288669-46tkedw7	282	10	clathrin	clathrin	NN
cord-288669-46tkedw7	282	11	-	-	HYPH
cord-288669-46tkedw7	282	12	like	like	JJ
cord-288669-46tkedw7	282	13	coated	coated	JJ
cord-288669-46tkedw7	282	14	vesicles	vesicle	NNS
cord-288669-46tkedw7	282	15	,	,	,
cord-288669-46tkedw7	282	16	the	the	DT
cord-288669-46tkedw7	282	17	virion	virion	NN
cord-288669-46tkedw7	282	18	particles	particle	NNS
cord-288669-46tkedw7	282	19	are	be	VBP
cord-288669-46tkedw7	282	20	transported	transport	VBN
cord-288669-46tkedw7	282	21	to	to	IN
cord-288669-46tkedw7	282	22	the	the	DT
cord-288669-46tkedw7	282	23	endosome	endosome	NN
cord-288669-46tkedw7	282	24	.	.	.
cord-288669-46tkedw7	283	1	There	there	RB
cord-288669-46tkedw7	283	2	,	,	,
cord-288669-46tkedw7	283	3	the	the	DT
cord-288669-46tkedw7	283	4	E	e	NN
cord-288669-46tkedw7	283	5	-	-	NN
cord-288669-46tkedw7	283	6	protein	protein	JJ
cord-288669-46tkedw7	283	7	ion	ion	NN
cord-288669-46tkedw7	283	8	channels	channel	NNS
cord-288669-46tkedw7	283	9	in	in	IN
cord-288669-46tkedw7	283	10	the	the	DT
cord-288669-46tkedw7	283	11	viral	viral	JJ
cord-288669-46tkedw7	283	12	membrane	membrane	NN
cord-288669-46tkedw7	283	13	undergo	undergo	VBP
cord-288669-46tkedw7	283	14	conformational	conformational	JJ
cord-288669-46tkedw7	283	15	changes	change	NNS
cord-288669-46tkedw7	283	16	upon	upon	IN
cord-288669-46tkedw7	283	17	exposure	exposure	NN
cord-288669-46tkedw7	283	18	to	to	IN
cord-288669-46tkedw7	283	19	low	low	JJ
cord-288669-46tkedw7	283	20	pH	ph	NN
cord-288669-46tkedw7	283	21	in	in	IN
cord-288669-46tkedw7	283	22	the	the	DT
cord-288669-46tkedw7	283	23	endosome	endosome	NN
cord-288669-46tkedw7	283	24	,	,	,
cord-288669-46tkedw7	283	25	and	and	CC
cord-288669-46tkedw7	283	26	allow	allow	VB
cord-288669-46tkedw7	283	27	ions	ion	NNS
cord-288669-46tkedw7	283	28	to	to	TO
cord-288669-46tkedw7	283	29	enter	enter	VB
cord-288669-46tkedw7	283	30	the	the	DT
cord-288669-46tkedw7	283	31	virion	virion	NN
cord-288669-46tkedw7	283	32	,	,	,
cord-288669-46tkedw7	283	33	which	which	WDT
cord-288669-46tkedw7	283	34	triggers	trigger	VBZ
cord-288669-46tkedw7	283	35	the	the	DT
cord-288669-46tkedw7	283	36	disassembly	disassembly	NN
cord-288669-46tkedw7	283	37	of	of	IN
cord-288669-46tkedw7	283	38	inner	inner	JJ
cord-288669-46tkedw7	283	39	capsid	capsid	NN
cord-288669-46tkedw7	283	40	and	and	CC
cord-288669-46tkedw7	283	41	the	the	DT
cord-288669-46tkedw7	283	42	release	release	NN
cord-288669-46tkedw7	283	43	of	of	IN
cord-288669-46tkedw7	283	44	viral	viral	JJ
cord-288669-46tkedw7	283	45	genome	genome	NN
cord-288669-46tkedw7	283	46	in	in	IN
cord-288669-46tkedw7	283	47	the	the	DT
cord-288669-46tkedw7	283	48	cytoplasm	cytoplasm	NN
cord-288669-46tkedw7	283	49	,	,	,
cord-288669-46tkedw7	283	50	such	such	JJ
cord-288669-46tkedw7	283	51	that	that	IN
cord-288669-46tkedw7	283	52	further	further	JJ
cord-288669-46tkedw7	283	53	steps	step	NNS
cord-288669-46tkedw7	283	54	of	of	IN
cord-288669-46tkedw7	283	55	genome	genome	NN
cord-288669-46tkedw7	283	56	replication	replication	NN
cord-288669-46tkedw7	283	57	and	and	CC
cord-288669-46tkedw7	283	58	infection	infection	NN
cord-288669-46tkedw7	283	59	cycle	cycle	NN
cord-288669-46tkedw7	283	60	can	can	MD
cord-288669-46tkedw7	283	61	proceed	proceed	VB
cord-288669-46tkedw7	283	62	.	.	.
cord-288669-46tkedw7	284	1	Marc-145	marc-145	DT
cord-288669-46tkedw7	285	1	(	(	-LRB-
cord-288669-46tkedw7	285	2	Kim	Kim	NNP
cord-288669-46tkedw7	285	3	et	et	NNP
cord-288669-46tkedw7	285	4	al	al	NNP
cord-288669-46tkedw7	285	5	.	.	NNP
cord-288669-46tkedw7	285	6	,	,	,
cord-288669-46tkedw7	285	7	1993	1993	CD
cord-288669-46tkedw7	285	8	)	)	-RRB-
cord-288669-46tkedw7	285	9	,	,	,
cord-288669-46tkedw7	285	10	HeLa	HeLa	NNP
cord-288669-46tkedw7	285	11	and	and	CC
cord-288669-46tkedw7	285	12	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	285	13	cells	cell	NNS
cord-288669-46tkedw7	285	14	were	be	VBD
cord-288669-46tkedw7	285	15	grown	grow	VBN
cord-288669-46tkedw7	285	16	in	in	IN
cord-288669-46tkedw7	285	17	Dulbecco	Dulbecco	NNP
cord-288669-46tkedw7	285	18	's	's	POS
cord-288669-46tkedw7	285	19	modified	modify	VBN
cord-288669-46tkedw7	285	20	Eagle	Eagle	NNP
cord-288669-46tkedw7	285	21	medium	medium	NN
cord-288669-46tkedw7	285	22	(	(	-LRB-
cord-288669-46tkedw7	285	23	DMEM	DMEM	NNP
cord-288669-46tkedw7	285	24	)	)	-RRB-
cord-288669-46tkedw7	285	25	supplemented	supplement	VBD
cord-288669-46tkedw7	285	26	with	with	IN
cord-288669-46tkedw7	285	27	8	8	CD
cord-288669-46tkedw7	285	28	%	%	NN
cord-288669-46tkedw7	285	29	fetal	fetal	JJ
cord-288669-46tkedw7	285	30	bovine	bovine	JJ
cord-288669-46tkedw7	285	31	serum	serum	NN
cord-288669-46tkedw7	285	32	(	(	-LRB-
cord-288669-46tkedw7	285	33	FBS	FBS	NNP
cord-288669-46tkedw7	285	34	;	;	:
cord-288669-46tkedw7	285	35	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	285	36	)	)	-RRB-
cord-288669-46tkedw7	285	37	,	,	,
cord-288669-46tkedw7	285	38	penicillin	penicillin	NN
cord-288669-46tkedw7	285	39	(	(	-LRB-
cord-288669-46tkedw7	285	40	100	100	CD
cord-288669-46tkedw7	285	41	U	U	NNP
cord-288669-46tkedw7	285	42	/	/	SYM
cord-288669-46tkedw7	285	43	ml	ml	NN
cord-288669-46tkedw7	285	44	)	)	-RRB-
cord-288669-46tkedw7	285	45	and	and	CC
cord-288669-46tkedw7	285	46	streptomycin	streptomycin	NNS
cord-288669-46tkedw7	285	47	(	(	-LRB-
cord-288669-46tkedw7	285	48	50	50	CD
cord-288669-46tkedw7	285	49	μg	μg	NN
cord-288669-46tkedw7	285	50	/	/	SYM
cord-288669-46tkedw7	285	51	ml	ml	NN
cord-288669-46tkedw7	285	52	)	)	-RRB-
cord-288669-46tkedw7	285	53	.	.	.
cord-288669-46tkedw7	286	1	BHK	BHK	NNP
cord-288669-46tkedw7	286	2	-	-	HYPH
cord-288669-46tkedw7	286	3	T7	t7	NN
cord-288669-46tkedw7	286	4	cells	cell	NNS
cord-288669-46tkedw7	286	5	stably	stably	RB
cord-288669-46tkedw7	286	6	expressing	express	VBG
cord-288669-46tkedw7	286	7	the	the	DT
cord-288669-46tkedw7	286	8	bacteriophage	bacteriophage	NN
cord-288669-46tkedw7	286	9	T7	T7	NNP
cord-288669-46tkedw7	286	10	RNA	RNA	NNP
cord-288669-46tkedw7	286	11	polymerase	polymerase	NN
cord-288669-46tkedw7	286	12	(	(	-LRB-
cord-288669-46tkedw7	286	13	generously	generously	RB
cord-288669-46tkedw7	286	14	provided	provide	VBN
cord-288669-46tkedw7	286	15	by	by	IN
cord-288669-46tkedw7	286	16	C.Y.	C.Y.	NNP
cord-288669-46tkedw7	286	17	Kang	Kang	NNP
cord-288669-46tkedw7	286	18	,	,	,
cord-288669-46tkedw7	286	19	University	University	NNP
cord-288669-46tkedw7	286	20	of	of	IN
cord-288669-46tkedw7	286	21	Western	Western	NNP
cord-288669-46tkedw7	286	22	Ontario	Ontario	NNP
cord-288669-46tkedw7	286	23	,	,	,
cord-288669-46tkedw7	286	24	London	London	NNP
cord-288669-46tkedw7	286	25	,	,	,
cord-288669-46tkedw7	286	26	Ontario	Ontario	NNP
cord-288669-46tkedw7	286	27	,	,	,
cord-288669-46tkedw7	286	28	Canada	Canada	NNP
cord-288669-46tkedw7	286	29	)	)	-RRB-
cord-288669-46tkedw7	286	30	were	be	VBD
cord-288669-46tkedw7	286	31	grown	grow	VBN
cord-288669-46tkedw7	286	32	in	in	IN
cord-288669-46tkedw7	286	33	DMEM	DMEM	NNP
cord-288669-46tkedw7	286	34	supplemented	supplement	VBD
cord-288669-46tkedw7	286	35	with	with	IN
cord-288669-46tkedw7	286	36	5	5	CD
cord-288669-46tkedw7	286	37	%	%	NN
cord-288669-46tkedw7	286	38	FBS	FBS	NNP
cord-288669-46tkedw7	286	39	,	,	,
cord-288669-46tkedw7	286	40	100	100	CD
cord-288669-46tkedw7	286	41	U	u	NN
cord-288669-46tkedw7	286	42	/	/	SYM
cord-288669-46tkedw7	286	43	ml	ml	NNS
cord-288669-46tkedw7	286	44	of	of	IN
cord-288669-46tkedw7	286	45	penicillin	penicillin	NN
cord-288669-46tkedw7	286	46	,	,	,
cord-288669-46tkedw7	286	47	50	50	CD
cord-288669-46tkedw7	286	48	μg	μg	NN
cord-288669-46tkedw7	286	49	/	/	SYM
cord-288669-46tkedw7	286	50	ml	ml	NNS
cord-288669-46tkedw7	286	51	of	of	IN
cord-288669-46tkedw7	286	52	streptomycin	streptomycin	NNS
cord-288669-46tkedw7	286	53	and	and	CC
cord-288669-46tkedw7	286	54	500	500	CD
cord-288669-46tkedw7	286	55	μg	μg	NN
cord-288669-46tkedw7	286	56	/	/	SYM
cord-288669-46tkedw7	286	57	ml	ml	NNS
cord-288669-46tkedw7	286	58	of	of	IN
cord-288669-46tkedw7	286	59	G418	G418	NNP
cord-288669-46tkedw7	286	60	(	(	-LRB-
cord-288669-46tkedw7	286	61	Geneticin	Geneticin	NNP
cord-288669-46tkedw7	286	62	;	;	:
cord-288669-46tkedw7	286	63	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	286	64	)	)	-RRB-
cord-288669-46tkedw7	286	65	.	.	.
cord-288669-46tkedw7	287	1	The	the	DT
cord-288669-46tkedw7	287	2	cells	cell	NNS
cord-288669-46tkedw7	287	3	were	be	VBD
cord-288669-46tkedw7	287	4	maintained	maintain	VBN
cord-288669-46tkedw7	287	5	at	at	IN
cord-288669-46tkedw7	287	6	37	37	CD
cord-288669-46tkedw7	287	7	°	°	NN
cord-288669-46tkedw7	287	8	C	c	NN
cord-288669-46tkedw7	287	9	with	with	IN
cord-288669-46tkedw7	287	10	5	5	CD
cord-288669-46tkedw7	287	11	%	%	NN
cord-288669-46tkedw7	287	12	CO	co	NN
cord-288669-46tkedw7	287	13	2	2	CD
cord-288669-46tkedw7	287	14	.	.	.
cord-288669-46tkedw7	288	1	Stocks	stock	NNS
cord-288669-46tkedw7	288	2	of	of	IN
cord-288669-46tkedw7	288	3	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	288	4	(	(	-LRB-
cord-288669-46tkedw7	288	5	strain	strain	NN
cord-288669-46tkedw7	288	6	PA8	PA8	NNP
cord-288669-46tkedw7	288	7	)	)	-RRB-
cord-288669-46tkedw7	288	8	and	and	CC
cord-288669-46tkedw7	289	1	recombinant	recombinant	JJ
cord-288669-46tkedw7	289	2	vaccinia	vaccinia	NN
cord-288669-46tkedw7	289	3	virus	virus	NN
cord-288669-46tkedw7	289	4	expressing	express	VBG
cord-288669-46tkedw7	289	5	T7	T7	NNP
cord-288669-46tkedw7	289	6	RNA	RNA	NNP
cord-288669-46tkedw7	289	7	polymerase	polymerase	NN
cord-288669-46tkedw7	289	8	(	(	-LRB-
cord-288669-46tkedw7	289	9	vTF7	vTF7	NNP
cord-288669-46tkedw7	289	10	-	-	HYPH
cord-288669-46tkedw7	289	11	3	3	CD
cord-288669-46tkedw7	289	12	;	;	:
cord-288669-46tkedw7	289	13	Fuerst	Fuerst	NNP
cord-288669-46tkedw7	289	14	et	et	NNP
cord-288669-46tkedw7	289	15	al	al	NNP
cord-288669-46tkedw7	289	16	.	.	NNP
cord-288669-46tkedw7	289	17	,	,	,
cord-288669-46tkedw7	289	18	1986	1986	CD
cord-288669-46tkedw7	289	19	)	)	-RRB-
cord-288669-46tkedw7	289	20	were	be	VBD
cord-288669-46tkedw7	289	21	prepared	prepare	VBN
cord-288669-46tkedw7	289	22	in	in	IN
cord-288669-46tkedw7	289	23	Marc-145	marc-145	CD
cord-288669-46tkedw7	289	24	or	or	CC
cord-288669-46tkedw7	289	25	HeLa	HeLa	NNP
cord-288669-46tkedw7	289	26	cells	cell	NNS
cord-288669-46tkedw7	289	27	,	,	,
cord-288669-46tkedw7	289	28	respectively	respectively	RB
cord-288669-46tkedw7	289	29	,	,	,
cord-288669-46tkedw7	289	30	as	as	IN
cord-288669-46tkedw7	289	31	described	describe	VBN
cord-288669-46tkedw7	289	32	previously	previously	RB
cord-288669-46tkedw7	289	33	(	(	-LRB-
cord-288669-46tkedw7	289	34	Wootton	Wootton	NNP
cord-288669-46tkedw7	289	35	et	et	NNP
cord-288669-46tkedw7	289	36	al	al	NNP
cord-288669-46tkedw7	289	37	.	.	NNP
cord-288669-46tkedw7	289	38	,	,	,
cord-288669-46tkedw7	289	39	2001	2001	CD
cord-288669-46tkedw7	289	40	)	)	-RRB-
cord-288669-46tkedw7	289	41	.	.	.
cord-288669-46tkedw7	290	1	The	the	DT
cord-288669-46tkedw7	290	2	N	n	CD
cord-288669-46tkedw7	290	3	protein	protein	NN
cord-288669-46tkedw7	290	4	-	-	HYPH
cord-288669-46tkedw7	290	5	specific	specific	JJ
cord-288669-46tkedw7	290	6	monoclonal	monoclonal	JJ
cord-288669-46tkedw7	290	7	antibody	antibody	NN
cord-288669-46tkedw7	290	8	(	(	-LRB-
cord-288669-46tkedw7	290	9	MAb	MAb	NNS
cord-288669-46tkedw7	290	10	)	)	-RRB-
cord-288669-46tkedw7	291	1	SDOW17	sdow17	XX
cord-288669-46tkedw7	291	2	,	,	,
cord-288669-46tkedw7	291	3	the	the	DT
cord-288669-46tkedw7	291	4	E	e	NN
cord-288669-46tkedw7	291	5	protein	protein	NN
cord-288669-46tkedw7	291	6	-	-	HYPH
cord-288669-46tkedw7	291	7	specific	specific	JJ
cord-288669-46tkedw7	291	8	anti	anti	JJ
cord-288669-46tkedw7	291	9	-	-	JJ
cord-288669-46tkedw7	291	10	peptide	peptide	JJ
cord-288669-46tkedw7	291	11	rabbit	rabbit	NN
cord-288669-46tkedw7	291	12	antiserum	antiserum	NN
cord-288669-46tkedw7	291	13	and	and	CC
cord-288669-46tkedw7	291	14	non	non	JJ
cord-288669-46tkedw7	291	15	-	-	JJ
cord-288669-46tkedw7	291	16	structural	structural	JJ
cord-288669-46tkedw7	291	17	proteins	protein	NNS
cord-288669-46tkedw7	291	18	2	2	CD
cord-288669-46tkedw7	291	19	and	and	CC
cord-288669-46tkedw7	291	20	3-specific	3-specific	CD
cord-288669-46tkedw7	291	21	polyclonal	polyclonal	JJ
cord-288669-46tkedw7	291	22	rabbit	rabbit	NN
cord-288669-46tkedw7	291	23	antiserum	antiserum	NN
cord-288669-46tkedw7	291	24	are	be	VBP
cord-288669-46tkedw7	291	25	described	describe	VBN
cord-288669-46tkedw7	291	26	elsewhere	elsewhere	RB
cord-288669-46tkedw7	291	27	Nelson	Nelson	NNP
cord-288669-46tkedw7	291	28	et	et	NNP
cord-288669-46tkedw7	291	29	al	al	NNP
cord-288669-46tkedw7	291	30	.	.	.
cord-288669-46tkedw7	292	1	,	,	,
cord-288669-46tkedw7	292	2	1993	1993	CD
cord-288669-46tkedw7	292	3	;	;	:
cord-288669-46tkedw7	292	4	Wootton	Wootton	NNP
cord-288669-46tkedw7	292	5	et	et	NNP
cord-288669-46tkedw7	292	6	al	al	NNP
cord-288669-46tkedw7	292	7	.	.	NNP
cord-288669-46tkedw7	292	8	,	,	,
cord-288669-46tkedw7	292	9	2001	2001	CD
cord-288669-46tkedw7	292	10	)	)	-RRB-
cord-288669-46tkedw7	292	11	.	.	.
cord-288669-46tkedw7	293	1	E.	E.	NNP
cord-288669-46tkedw7	293	2	coli	coli	VBZ
cord-288669-46tkedw7	293	3	strains	strain	NNS
cord-288669-46tkedw7	293	4	XL1-Blue	XL1-Blue	NNS
cord-288669-46tkedw7	293	5	(	(	-LRB-
cord-288669-46tkedw7	293	6	Stratagene	Stratagene	NNP
cord-288669-46tkedw7	293	7	)	)	-RRB-
cord-288669-46tkedw7	293	8	and	and	CC
cord-288669-46tkedw7	293	9	DH5α	DH5α	NNP
cord-288669-46tkedw7	293	10	were	be	VBD
cord-288669-46tkedw7	293	11	used	use	VBN
cord-288669-46tkedw7	293	12	as	as	IN
cord-288669-46tkedw7	293	13	hosts	host	NNS
cord-288669-46tkedw7	293	14	for	for	IN
cord-288669-46tkedw7	293	15	site	site	NN
cord-288669-46tkedw7	293	16	-	-	HYPH
cord-288669-46tkedw7	293	17	directed	direct	VBN
cord-288669-46tkedw7	293	18	mutagenesis	mutagenesis	NN
cord-288669-46tkedw7	293	19	and	and	CC
cord-288669-46tkedw7	293	20	general	general	JJ
cord-288669-46tkedw7	293	21	cloning	cloning	NN
cord-288669-46tkedw7	293	22	,	,	,
cord-288669-46tkedw7	293	23	respectively	respectively	RB
cord-288669-46tkedw7	293	24	.	.	.
cord-288669-46tkedw7	294	1	cDNA	cdna	NN
cord-288669-46tkedw7	294	2	cloning	cloning	NN
cord-288669-46tkedw7	294	3	of	of	IN
cord-288669-46tkedw7	294	4	the	the	DT
cord-288669-46tkedw7	294	5	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	294	6	N	N	NNP
cord-288669-46tkedw7	294	7	or	or	CC
cord-288669-46tkedw7	294	8	E	e	NN
cord-288669-46tkedw7	294	9	gene	gene	NN
cord-288669-46tkedw7	294	10	to	to	TO
cord-288669-46tkedw7	294	11	produce	produce	VB
cord-288669-46tkedw7	294	12	pCITE	pCITE	NNP
cord-288669-46tkedw7	294	13	-	-	HYPH
cord-288669-46tkedw7	294	14	N	n	NN
cord-288669-46tkedw7	294	15	;	;	:
cord-288669-46tkedw7	294	16	pGEX	pGEX	NNP
cord-288669-46tkedw7	294	17	-	-	HYPH
cord-288669-46tkedw7	294	18	N	n	CD
cord-288669-46tkedw7	294	19	or	or	CC
cord-288669-46tkedw7	294	20	pCITE	pcite	NN
cord-288669-46tkedw7	294	21	-	-	HYPH
cord-288669-46tkedw7	294	22	E	e	NN
cord-288669-46tkedw7	294	23	;	;	,
cord-288669-46tkedw7	294	24	pGEX	pgex	JJ
cord-288669-46tkedw7	294	25	-	-	HYPH
cord-288669-46tkedw7	294	26	E	e	NN
cord-288669-46tkedw7	294	27	,	,	,
cord-288669-46tkedw7	294	28	respectively	respectively	RB
cord-288669-46tkedw7	294	29	,	,	,
cord-288669-46tkedw7	294	30	is	be	VBZ
cord-288669-46tkedw7	294	31	described	describe	VBN
cord-288669-46tkedw7	294	32	elsewhere	elsewhere	RB
cord-288669-46tkedw7	294	33	(	(	-LRB-
cord-288669-46tkedw7	294	34	Wootton	Wootton	NNP
cord-288669-46tkedw7	294	35	and	and	CC
cord-288669-46tkedw7	294	36	Yoo	Yoo	NNP
cord-288669-46tkedw7	294	37	,	,	,
cord-288669-46tkedw7	294	38	2003	2003	CD
cord-288669-46tkedw7	294	39	;	;	:
cord-288669-46tkedw7	294	40	.	.	.
cord-288669-46tkedw7	295	1	To	to	TO
cord-288669-46tkedw7	295	2	modify	modify	VB
cord-288669-46tkedw7	295	3	the	the	DT
cord-288669-46tkedw7	295	4	translational	translational	JJ
cord-288669-46tkedw7	295	5	initiation	initiation	NN
cord-288669-46tkedw7	295	6	codon	codon	NN
cord-288669-46tkedw7	295	7	of	of	IN
cord-288669-46tkedw7	295	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	295	9	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	296	1	(	(	-LRB-
cord-288669-46tkedw7	296	2	E	E	NNP
cord-288669-46tkedw7	296	3	)	)	-RRB-
cord-288669-46tkedw7	296	4	,	,	,
cord-288669-46tkedw7	296	5	PCR	PCR	NNP
cord-288669-46tkedw7	296	6	-	-	HYPH
cord-288669-46tkedw7	296	7	based	base	VBN
cord-288669-46tkedw7	296	8	site	site	NN
cord-288669-46tkedw7	296	9	-	-	HYPH
cord-288669-46tkedw7	296	10	directed	direct	VBN
cord-288669-46tkedw7	296	11	mutagenesis	mutagenesis	NN
cord-288669-46tkedw7	296	12	was	be	VBD
cord-288669-46tkedw7	296	13	first	first	RB
cord-288669-46tkedw7	296	14	conducted	conduct	VBN
cord-288669-46tkedw7	296	15	to	to	TO
cord-288669-46tkedw7	296	16	mutate	mutate	VB
cord-288669-46tkedw7	296	17	the	the	DT
cord-288669-46tkedw7	296	18	ATG	ATG	NNP
cord-288669-46tkedw7	296	19	start	start	NN
cord-288669-46tkedw7	296	20	codon	codon	NN
cord-288669-46tkedw7	296	21	of	of	IN
cord-288669-46tkedw7	296	22	the	the	DT
cord-288669-46tkedw7	296	23	E	e	NN
cord-288669-46tkedw7	296	24	gene	gene	NN
cord-288669-46tkedw7	296	25	to	to	IN
cord-288669-46tkedw7	296	26	GTG	GTG	NNP
cord-288669-46tkedw7	296	27	at	at	IN
cord-288669-46tkedw7	296	28	genomic	genomic	JJ
cord-288669-46tkedw7	296	29	nucleotide	nucleotide	JJ
cord-288669-46tkedw7	296	30	positions	position	NNS
cord-288669-46tkedw7	296	31	12,062	12,062	CD
cord-288669-46tkedw7	296	32	to	to	IN
cord-288669-46tkedw7	296	33	12,064	12,064	CD
cord-288669-46tkedw7	296	34	using	use	VBG
cord-288669-46tkedw7	296	35	the	the	DT
cord-288669-46tkedw7	296	36	shuttle	shuttle	NN
cord-288669-46tkedw7	296	37	vector	vector	NN
cord-288669-46tkedw7	296	38	pTB	pTB	''
cord-288669-46tkedw7	296	39	-	-	HYPH
cord-288669-46tkedw7	296	40	shuttle	shuttle	NN
cord-288669-46tkedw7	296	41	-	-	HYPH
cord-288669-46tkedw7	296	42	PRRSV-3997	PRRSV-3997	VBN
cord-288669-46tkedw7	296	43	with	with	IN
cord-288669-46tkedw7	296	44	the	the	DT
cord-288669-46tkedw7	296	45	following	follow	VBG
cord-288669-46tkedw7	296	46	primer	primer	NN
cord-288669-46tkedw7	296	47	pairs	pair	NNS
cord-288669-46tkedw7	296	48	;	;	:
cord-288669-46tkedw7	296	49	for	for	IN
cord-288669-46tkedw7	296	50	A12062	A12062	NNP
cord-288669-46tkedw7	296	51	G	G	NNP
cord-288669-46tkedw7	296	52	mutation	mutation	NN
cord-288669-46tkedw7	296	53	,	,	,
cord-288669-46tkedw7	296	54	E	E	NNP
cord-288669-46tkedw7	296	55	-	-	NNP
cord-288669-46tkedw7	296	56	KO	KO	NNP
cord-288669-46tkedw7	296	57	-	-	HYPH
cord-288669-46tkedw7	296	58	Fwd	Fwd	NNP
cord-288669-46tkedw7	296	59	(	(	-LRB-
cord-288669-46tkedw7	296	60	5′-GAATTGAAATGAAgTGGGGTCTATAC-3′	5′-gaattgaaatgaagtggggtctatac-3′	CD
cord-288669-46tkedw7	296	61	:	:	:
cord-288669-46tkedw7	296	62	nucleotide	nucleotide	JJ
cord-288669-46tkedw7	296	63	positions	position	NNS
cord-288669-46tkedw7	296	64	12,049	12,049	CD
cord-288669-46tkedw7	296	65	to	to	IN
cord-288669-46tkedw7	296	66	12,074	12,074	CD
cord-288669-46tkedw7	296	67	)	)	-RRB-
cord-288669-46tkedw7	296	68	and	and	CC
cord-288669-46tkedw7	296	69	E	E	NNP
cord-288669-46tkedw7	296	70	-	-	NN
cord-288669-46tkedw7	296	71	KO	KO	NNP
cord-288669-46tkedw7	296	72	-	-	HYPH
cord-288669-46tkedw7	296	73	Rev	Rev	NNP
cord-288669-46tkedw7	296	74	(	(	-LRB-
cord-288669-46tkedw7	296	75	5′-GTATA	5′-GTATA	NNP
cord-288669-46tkedw7	296	76	-	-	HYPH
cord-288669-46tkedw7	296	77	GACCCCAcTTCATTTCAATTC-3′	GACCCCAcTTCATTTCAATTC-3′	NNP
cord-288669-46tkedw7	296	78	:	:	:
cord-288669-46tkedw7	296	79	nucleotide	nucleotide	JJ
cord-288669-46tkedw7	296	80	positions	position	NNS
cord-288669-46tkedw7	296	81	12,049	12,049	CD
cord-288669-46tkedw7	296	82	to	to	IN
cord-288669-46tkedw7	296	83	12,074	12,074	CD
cord-288669-46tkedw7	296	84	)	)	-RRB-
cord-288669-46tkedw7	296	85	,	,	,
cord-288669-46tkedw7	296	86	where	where	WRB
cord-288669-46tkedw7	296	87	lowercase	lowercase	NN
cord-288669-46tkedw7	296	88	letters	letter	NNS
cord-288669-46tkedw7	296	89	indicate	indicate	VBP
cord-288669-46tkedw7	296	90	mutated	mutate	VBN
cord-288669-46tkedw7	296	91	nucleotides	nucleotide	NNS
cord-288669-46tkedw7	296	92	.	.	.
cord-288669-46tkedw7	297	1	The	the	DT
cord-288669-46tkedw7	297	2	A12062	A12062	NNP
cord-288669-46tkedw7	297	3	G	g	NN
cord-288669-46tkedw7	297	4	mutation	mutation	NN
cord-288669-46tkedw7	297	5	was	be	VBD
cord-288669-46tkedw7	297	6	translationally	translationally	RB
cord-288669-46tkedw7	297	7	silent	silent	JJ
cord-288669-46tkedw7	297	8	with	with	IN
cord-288669-46tkedw7	297	9	respect	respect	NN
cord-288669-46tkedw7	297	10	to	to	IN
cord-288669-46tkedw7	297	11	ORF2a	orf2a	NN
cord-288669-46tkedw7	297	12	encoding	encode	VBG
cord-288669-46tkedw7	297	13	the	the	DT
cord-288669-46tkedw7	297	14	GP2	GP2	NNP
cord-288669-46tkedw7	297	15	protein	protein	NN
cord-288669-46tkedw7	297	16	.	.	.
cord-288669-46tkedw7	298	1	PCR	PCR	NNP
cord-288669-46tkedw7	298	2	-	-	HYPH
cord-288669-46tkedw7	298	3	based	base	VBN
cord-288669-46tkedw7	298	4	mutagenesis	mutagenesis	NN
cord-288669-46tkedw7	298	5	and	and	CC
cord-288669-46tkedw7	298	6	screening	screening	NN
cord-288669-46tkedw7	298	7	of	of	IN
cord-288669-46tkedw7	298	8	mutants	mutant	NNS
cord-288669-46tkedw7	298	9	were	be	VBD
cord-288669-46tkedw7	298	10	performed	perform	VBN
cord-288669-46tkedw7	298	11	as	as	IN
cord-288669-46tkedw7	298	12	described	describe	VBN
cord-288669-46tkedw7	298	13	previously	previously	RB
cord-288669-46tkedw7	298	14	(	(	-LRB-
cord-288669-46tkedw7	298	15	Wootton	Wootton	NNP
cord-288669-46tkedw7	298	16	et	et	NNP
cord-288669-46tkedw7	298	17	al	al	NNP
cord-288669-46tkedw7	298	18	.	.	NNP
cord-288669-46tkedw7	298	19	,	,	,
cord-288669-46tkedw7	298	20	2001	2001	CD
cord-288669-46tkedw7	298	21	)	)	-RRB-
cord-288669-46tkedw7	298	22	.	.	.
cord-288669-46tkedw7	299	1	The	the	DT
cord-288669-46tkedw7	299	2	shuttle	shuttle	NN
cord-288669-46tkedw7	299	3	plasmid	plasmid	NN
cord-288669-46tkedw7	299	4	carrying	carry	VBG
cord-288669-46tkedw7	299	5	the	the	DT
cord-288669-46tkedw7	299	6	A12062	A12062	NNP
cord-288669-46tkedw7	299	7	G	g	NN
cord-288669-46tkedw7	299	8	mutation	mutation	NN
cord-288669-46tkedw7	299	9	was	be	VBD
cord-288669-46tkedw7	299	10	digested	digest	VBN
cord-288669-46tkedw7	299	11	with	with	IN
cord-288669-46tkedw7	299	12	Eco47III	Eco47III	NNP
cord-288669-46tkedw7	299	13	and	and	CC
cord-288669-46tkedw7	299	14	BsrGI	BsrGI	NNP
cord-288669-46tkedw7	299	15	,	,	,
cord-288669-46tkedw7	299	16	and	and	CC
cord-288669-46tkedw7	299	17	a	a	DT
cord-288669-46tkedw7	299	18	908-bp	908-bp	CD
cord-288669-46tkedw7	299	19	fragment	fragment	NN
cord-288669-46tkedw7	299	20	was	be	VBD
cord-288669-46tkedw7	299	21	purified	purify	VBN
cord-288669-46tkedw7	299	22	.	.	.
cord-288669-46tkedw7	300	1	The	the	DT
cord-288669-46tkedw7	300	2	wild	wild	JJ
cord-288669-46tkedw7	300	3	-	-	HYPH
cord-288669-46tkedw7	300	4	type	type	NN
cord-288669-46tkedw7	300	5	full	full	JJ
cord-288669-46tkedw7	300	6	-	-	HYPH
cord-288669-46tkedw7	300	7	length	length	NN
cord-288669-46tkedw7	300	8	genomic	genomic	JJ
cord-288669-46tkedw7	300	9	cDNA	cdna	NN
cord-288669-46tkedw7	300	10	clone	clone	NN
cord-288669-46tkedw7	300	11	was	be	VBD
cord-288669-46tkedw7	300	12	digested	digest	VBN
cord-288669-46tkedw7	300	13	with	with	IN
cord-288669-46tkedw7	300	14	Eco47III	Eco47III	NNP
cord-288669-46tkedw7	300	15	and	and	CC
cord-288669-46tkedw7	300	16	BsrGI	BsrGI	NNP
cord-288669-46tkedw7	300	17	,	,	,
cord-288669-46tkedw7	300	18	and	and	CC
cord-288669-46tkedw7	300	19	the	the	DT
cord-288669-46tkedw7	300	20	908-bp	908-bp	CD
cord-288669-46tkedw7	300	21	Eco47III	Eco47III	NNP
cord-288669-46tkedw7	300	22	-	-	HYPH
cord-288669-46tkedw7	300	23	BsrGI	BsrGI	NNP
cord-288669-46tkedw7	300	24	fragment	fragment	NN
cord-288669-46tkedw7	300	25	was	be	VBD
cord-288669-46tkedw7	300	26	replaced	replace	VBN
cord-288669-46tkedw7	300	27	with	with	IN
cord-288669-46tkedw7	300	28	the	the	DT
cord-288669-46tkedw7	300	29	corresponding	corresponding	JJ
cord-288669-46tkedw7	300	30	fragment	fragment	NN
cord-288669-46tkedw7	300	31	obtained	obtain	VBN
cord-288669-46tkedw7	300	32	from	from	IN
cord-288669-46tkedw7	300	33	the	the	DT
cord-288669-46tkedw7	300	34	shuttle	shuttle	NN
cord-288669-46tkedw7	300	35	plasmid	plasmid	NN
cord-288669-46tkedw7	300	36	.	.	.
cord-288669-46tkedw7	301	1	The	the	DT
cord-288669-46tkedw7	301	2	ligated	ligate	VBN
cord-288669-46tkedw7	301	3	full	full	JJ
cord-288669-46tkedw7	301	4	-	-	HYPH
cord-288669-46tkedw7	301	5	length	length	NN
cord-288669-46tkedw7	301	6	plasmid	plasmid	NN
cord-288669-46tkedw7	301	7	DNA	DNA	NNP
cord-288669-46tkedw7	301	8	was	be	VBD
cord-288669-46tkedw7	301	9	screened	screen	VBN
cord-288669-46tkedw7	301	10	by	by	IN
cord-288669-46tkedw7	301	11	XmaI	XmaI	NNP
cord-288669-46tkedw7	301	12	digestion	digestion	NN
cord-288669-46tkedw7	301	13	,	,	,
cord-288669-46tkedw7	301	14	and	and	CC
cord-288669-46tkedw7	301	15	based	base	VBN
cord-288669-46tkedw7	301	16	on	on	IN
cord-288669-46tkedw7	301	17	the	the	DT
cord-288669-46tkedw7	301	18	XmaI	XmaI	NNP
cord-288669-46tkedw7	301	19	digestion	digestion	NN
cord-288669-46tkedw7	301	20	pattern	pattern	NN
cord-288669-46tkedw7	301	21	,	,	,
cord-288669-46tkedw7	301	22	positive	positive	JJ
cord-288669-46tkedw7	301	23	clones	clone	NNS
cord-288669-46tkedw7	301	24	were	be	VBD
cord-288669-46tkedw7	301	25	selected	select	VBN
cord-288669-46tkedw7	301	26	.	.	.
cord-288669-46tkedw7	302	1	DNA	dna	NN
cord-288669-46tkedw7	302	2	manipulation	manipulation	NN
cord-288669-46tkedw7	302	3	and	and	CC
cord-288669-46tkedw7	302	4	cloning	cloning	NN
cord-288669-46tkedw7	302	5	were	be	VBD
cord-288669-46tkedw7	302	6	performed	perform	VBN
cord-288669-46tkedw7	302	7	according	accord	VBG
cord-288669-46tkedw7	302	8	to	to	IN
cord-288669-46tkedw7	302	9	standard	standard	JJ
cord-288669-46tkedw7	302	10	procedures	procedure	NNS
cord-288669-46tkedw7	302	11	(	(	-LRB-
cord-288669-46tkedw7	302	12	Sambrook	Sambrook	NNP
cord-288669-46tkedw7	302	13	and	and	CC
cord-288669-46tkedw7	302	14	Russell	Russell	NNP
cord-288669-46tkedw7	302	15	,	,	,
cord-288669-46tkedw7	302	16	2001	2001	CD
cord-288669-46tkedw7	302	17	)	)	-RRB-
cord-288669-46tkedw7	302	18	.	.	.
cord-288669-46tkedw7	303	1	The	the	DT
cord-288669-46tkedw7	303	2	selected	select	VBN
cord-288669-46tkedw7	303	3	clones	clone	NNS
cord-288669-46tkedw7	303	4	were	be	VBD
cord-288669-46tkedw7	303	5	sequenced	sequence	VBN
cord-288669-46tkedw7	303	6	to	to	TO
cord-288669-46tkedw7	303	7	confirm	confirm	VB
cord-288669-46tkedw7	303	8	the	the	DT
cord-288669-46tkedw7	303	9	presence	presence	NN
cord-288669-46tkedw7	303	10	of	of	IN
cord-288669-46tkedw7	303	11	the	the	DT
cord-288669-46tkedw7	303	12	A12062	A12062	NNP
cord-288669-46tkedw7	303	13	G	G	NNP
cord-288669-46tkedw7	303	14	mutation	mutation	NN
cord-288669-46tkedw7	303	15	in	in	IN
cord-288669-46tkedw7	303	16	the	the	DT
cord-288669-46tkedw7	303	17	full	full	JJ
cord-288669-46tkedw7	303	18	-	-	HYPH
cord-288669-46tkedw7	303	19	length	length	NN
cord-288669-46tkedw7	303	20	genomic	genomic	JJ
cord-288669-46tkedw7	303	21	cDNA	cdna	NN
cord-288669-46tkedw7	303	22	clone	clone	NN
cord-288669-46tkedw7	303	23	.	.	.
cord-288669-46tkedw7	304	1	The	the	DT
cord-288669-46tkedw7	304	2	resulting	result	VBG
cord-288669-46tkedw7	304	3	plasmid	plasmid	NN
cord-288669-46tkedw7	304	4	was	be	VBD
cord-288669-46tkedw7	304	5	designated	designate	VBN
cord-288669-46tkedw7	304	6	pCMV	pcmv	NN
cord-288669-46tkedw7	304	7	-	-	HYPH
cord-288669-46tkedw7	304	8	S	s	NN
cord-288669-46tkedw7	304	9	-	-	HYPH
cord-288669-46tkedw7	304	10	P129-ΔE	P129-ΔE	NNP
cord-288669-46tkedw7	304	11	.	.	.
cord-288669-46tkedw7	305	1	Marc-145	marc-145	DT
cord-288669-46tkedw7	305	2	cells	cell	NNS
cord-288669-46tkedw7	305	3	or	or	CC
cord-288669-46tkedw7	305	4	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	305	5	cells	cell	NNS
cord-288669-46tkedw7	305	6	were	be	VBD
cord-288669-46tkedw7	305	7	seeded	seed	VBN
cord-288669-46tkedw7	305	8	on	on	IN
cord-288669-46tkedw7	305	9	microscope	microscope	NN
cord-288669-46tkedw7	305	10	coverslips	coverslip	NNS
cord-288669-46tkedw7	305	11	placed	place	VBN
cord-288669-46tkedw7	305	12	in	in	IN
cord-288669-46tkedw7	305	13	35-mm	35-mm	CD
cord-288669-46tkedw7	305	14	-	-	HYPH
cord-288669-46tkedw7	305	15	diameter	diameter	NN
cord-288669-46tkedw7	305	16	dishes	dish	NNS
cord-288669-46tkedw7	305	17	and	and	CC
cord-288669-46tkedw7	305	18	grown	grow	VBN
cord-288669-46tkedw7	305	19	overnight	overnight	RB
cord-288669-46tkedw7	305	20	to	to	IN
cord-288669-46tkedw7	305	21	70	70	CD
cord-288669-46tkedw7	305	22	%	%	NN
cord-288669-46tkedw7	305	23	confluence	confluence	NN
cord-288669-46tkedw7	305	24	.	.	.
cord-288669-46tkedw7	306	1	The	the	DT
cord-288669-46tkedw7	306	2	cells	cell	NNS
cord-288669-46tkedw7	306	3	were	be	VBD
cord-288669-46tkedw7	306	4	transfected	transfecte	VBN
cord-288669-46tkedw7	306	5	with	with	IN
cord-288669-46tkedw7	306	6	2	2	CD
cord-288669-46tkedw7	306	7	μg	μg	CD
cord-288669-46tkedw7	306	8	of	of	IN
cord-288669-46tkedw7	306	9	plasmid	plasmid	NN
cord-288669-46tkedw7	306	10	DNA	dna	NN
cord-288669-46tkedw7	306	11	using	use	VBG
cord-288669-46tkedw7	306	12	Lipofectin	Lipofectin	NNP
cord-288669-46tkedw7	306	13	(	(	-LRB-
cord-288669-46tkedw7	306	14	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	306	15	)	)	-RRB-
cord-288669-46tkedw7	306	16	according	accord	VBG
cord-288669-46tkedw7	306	17	to	to	IN
cord-288669-46tkedw7	306	18	the	the	DT
cord-288669-46tkedw7	306	19	manufacturer	manufacturer	NN
cord-288669-46tkedw7	306	20	's	's	POS
cord-288669-46tkedw7	306	21	instructions	instruction	NNS
cord-288669-46tkedw7	306	22	.	.	.
cord-288669-46tkedw7	307	1	At	at	IN
cord-288669-46tkedw7	307	2	48	48	CD
cord-288669-46tkedw7	307	3	h	h	NN
cord-288669-46tkedw7	307	4	post	post	NN
cord-288669-46tkedw7	307	5	-	-	NN
cord-288669-46tkedw7	307	6	transfection	transfection	JJ
cord-288669-46tkedw7	307	7	,	,	,
cord-288669-46tkedw7	307	8	cell	cell	NN
cord-288669-46tkedw7	307	9	monolayers	monolayer	NNS
cord-288669-46tkedw7	307	10	were	be	VBD
cord-288669-46tkedw7	307	11	washed	wash	VBN
cord-288669-46tkedw7	307	12	twice	twice	RB
cord-288669-46tkedw7	307	13	in	in	IN
cord-288669-46tkedw7	307	14	PBS	PBS	NNP
cord-288669-46tkedw7	307	15	and	and	CC
cord-288669-46tkedw7	307	16	fixed	fix	VBN
cord-288669-46tkedw7	307	17	immediately	immediately	RB
cord-288669-46tkedw7	307	18	with	with	IN
cord-288669-46tkedw7	307	19	cold	cold	JJ
cord-288669-46tkedw7	307	20	methanol	methanol	NN
cord-288669-46tkedw7	307	21	for	for	IN
cord-288669-46tkedw7	307	22	10	10	CD
cord-288669-46tkedw7	307	23	min	min	NN
cord-288669-46tkedw7	307	24	at	at	IN
cord-288669-46tkedw7	307	25	−	−	CD
cord-288669-46tkedw7	307	26	20	20	CD
cord-288669-46tkedw7	307	27	°	°	NN
cord-288669-46tkedw7	307	28	C	c	NN
cord-288669-46tkedw7	307	29	.	.	.
cord-288669-46tkedw7	308	1	For	for	IN
cord-288669-46tkedw7	308	2	time	time	NN
cord-288669-46tkedw7	308	3	course	course	NN
cord-288669-46tkedw7	308	4	experiments	experiment	NNS
cord-288669-46tkedw7	308	5	,	,	,
cord-288669-46tkedw7	308	6	Marc-145	marc-145	NN
cord-288669-46tkedw7	308	7	cells	cell	NNS
cord-288669-46tkedw7	308	8	inoculated	inoculate	VBN
cord-288669-46tkedw7	308	9	with	with	IN
cord-288669-46tkedw7	308	10	purified	purify	VBN
cord-288669-46tkedw7	308	11	passage-1	passage-1	NNP
cord-288669-46tkedw7	308	12	viruses	virus	NNS
cord-288669-46tkedw7	308	13	prepared	prepare	VBN
cord-288669-46tkedw7	308	14	in	in	IN
cord-288669-46tkedw7	308	15	the	the	DT
cord-288669-46tkedw7	308	16	transfected	transfecte	VBN
cord-288669-46tkedw7	308	17	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	308	18	cells	cell	NNS
cord-288669-46tkedw7	308	19	were	be	VBD
cord-288669-46tkedw7	308	20	fixed	fix	VBN
cord-288669-46tkedw7	308	21	at	at	IN
cord-288669-46tkedw7	308	22	various	various	JJ
cord-288669-46tkedw7	308	23	time	time	NN
cord-288669-46tkedw7	308	24	points	point	NNS
cord-288669-46tkedw7	308	25	after	after	IN
cord-288669-46tkedw7	308	26	infection	infection	NN
cord-288669-46tkedw7	308	27	.	.	.
cord-288669-46tkedw7	309	1	Cells	cell	NNS
cord-288669-46tkedw7	309	2	were	be	VBD
cord-288669-46tkedw7	309	3	blocked	block	VBN
cord-288669-46tkedw7	309	4	using	use	VBG
cord-288669-46tkedw7	309	5	1	1	CD
cord-288669-46tkedw7	309	6	%	%	NN
cord-288669-46tkedw7	309	7	bovine	bovine	JJ
cord-288669-46tkedw7	309	8	serum	serum	NN
cord-288669-46tkedw7	309	9	albumin	albumin	NN
cord-288669-46tkedw7	309	10	(	(	-LRB-
cord-288669-46tkedw7	309	11	BSA	BSA	NNP
cord-288669-46tkedw7	309	12	)	)	-RRB-
cord-288669-46tkedw7	309	13	in	in	IN
cord-288669-46tkedw7	309	14	PBS	PBS	NNP
cord-288669-46tkedw7	309	15	for	for	IN
cord-288669-46tkedw7	309	16	30	30	CD
cord-288669-46tkedw7	309	17	min	min	NN
cord-288669-46tkedw7	309	18	at	at	IN
cord-288669-46tkedw7	309	19	room	room	NN
cord-288669-46tkedw7	309	20	temperature	temperature	NN
cord-288669-46tkedw7	309	21	(	(	-LRB-
cord-288669-46tkedw7	309	22	RT	RT	NNP
cord-288669-46tkedw7	309	23	)	)	-RRB-
cord-288669-46tkedw7	309	24	and	and	CC
cord-288669-46tkedw7	309	25	then	then	RB
cord-288669-46tkedw7	309	26	incubated	incubate	VBD
cord-288669-46tkedw7	309	27	with	with	IN
cord-288669-46tkedw7	309	28	N	n	CD
cord-288669-46tkedw7	309	29	-	-	HYPH
cord-288669-46tkedw7	309	30	specific	specific	JJ
cord-288669-46tkedw7	309	31	MAb	MAb	NNS
cord-288669-46tkedw7	309	32	SDOW17	sdow17	XX
cord-288669-46tkedw7	309	33	for	for	IN
cord-288669-46tkedw7	309	34	2	2	CD
cord-288669-46tkedw7	309	35	h.	h.	NN
cord-288669-46tkedw7	309	36	After	after	IN
cord-288669-46tkedw7	309	37	washing	wash	VBG
cord-288669-46tkedw7	309	38	five	five	CD
cord-288669-46tkedw7	309	39	times	time	NNS
cord-288669-46tkedw7	309	40	in	in	IN
cord-288669-46tkedw7	309	41	PBS	PBS	NNP
cord-288669-46tkedw7	309	42	,	,	,
cord-288669-46tkedw7	309	43	the	the	DT
cord-288669-46tkedw7	309	44	cells	cell	NNS
cord-288669-46tkedw7	309	45	were	be	VBD
cord-288669-46tkedw7	309	46	incubated	incubate	VBN
cord-288669-46tkedw7	309	47	for	for	IN
cord-288669-46tkedw7	309	48	1	1	CD
cord-288669-46tkedw7	309	49	h	h	NN
cord-288669-46tkedw7	309	50	at	at	IN
cord-288669-46tkedw7	309	51	room	room	NN
cord-288669-46tkedw7	309	52	temperature	temperature	NN
cord-288669-46tkedw7	309	53	with	with	IN
cord-288669-46tkedw7	309	54	goat	goat	NN
cord-288669-46tkedw7	309	55	antimouse	antimouse	IN
cord-288669-46tkedw7	309	56	secondary	secondary	JJ
cord-288669-46tkedw7	309	57	antibody	antibody	NN
cord-288669-46tkedw7	309	58	conjugated	conjugate	VBN
cord-288669-46tkedw7	309	59	with	with	IN
cord-288669-46tkedw7	309	60	Alexa	Alexa	NNP
cord-288669-46tkedw7	309	61	green	green	JJ
cord-288669-46tkedw7	309	62	dye	dye	NN
cord-288669-46tkedw7	309	63	(	(	-LRB-
cord-288669-46tkedw7	309	64	Molecular	Molecular	NNP
cord-288669-46tkedw7	309	65	Probes	Probes	NNPS
cord-288669-46tkedw7	309	66	)	)	-RRB-
cord-288669-46tkedw7	309	67	.	.	.
cord-288669-46tkedw7	310	1	For	for	IN
cord-288669-46tkedw7	310	2	dual	dual	JJ
cord-288669-46tkedw7	310	3	immunofluorescence	immunofluorescence	NN
cord-288669-46tkedw7	310	4	,	,	,
cord-288669-46tkedw7	310	5	cells	cell	NNS
cord-288669-46tkedw7	310	6	were	be	VBD
cord-288669-46tkedw7	310	7	co	co	JJ
cord-288669-46tkedw7	310	8	-	-	JJ
cord-288669-46tkedw7	310	9	stained	stain	VBN
cord-288669-46tkedw7	310	10	with	with	IN
cord-288669-46tkedw7	310	11	nsp2/3-specific	nsp2/3-specific	CD
cord-288669-46tkedw7	310	12	rabbit	rabbit	NN
cord-288669-46tkedw7	310	13	antiserum	antiserum	NN
cord-288669-46tkedw7	310	14	and	and	CC
cord-288669-46tkedw7	310	15	N	n	CD
cord-288669-46tkedw7	310	16	-	-	HYPH
cord-288669-46tkedw7	310	17	specific	specific	JJ
cord-288669-46tkedw7	310	18	MAb	MAb	NNS
cord-288669-46tkedw7	310	19	SDOW17	sdow17	XX
cord-288669-46tkedw7	310	20	,	,	,
cord-288669-46tkedw7	310	21	followed	follow	VBN
cord-288669-46tkedw7	310	22	by	by	IN
cord-288669-46tkedw7	310	23	incubation	incubation	NN
cord-288669-46tkedw7	310	24	with	with	IN
cord-288669-46tkedw7	310	25	goat	goat	NN
cord-288669-46tkedw7	310	26	anti	anti	JJ
cord-288669-46tkedw7	310	27	-	-	JJ
cord-288669-46tkedw7	310	28	rabbit	rabbit	JJ
cord-288669-46tkedw7	310	29	antibody	antibody	NN
cord-288669-46tkedw7	310	30	conjugated	conjugate	VBN
cord-288669-46tkedw7	310	31	with	with	IN
cord-288669-46tkedw7	310	32	Texas	Texas	NNP
cord-288669-46tkedw7	310	33	red	red	NN
cord-288669-46tkedw7	310	34	(	(	-LRB-
cord-288669-46tkedw7	310	35	Molecular	Molecular	NNP
cord-288669-46tkedw7	310	36	Probes	Probes	NNPS
cord-288669-46tkedw7	310	37	)	)	-RRB-
cord-288669-46tkedw7	310	38	and	and	CC
cord-288669-46tkedw7	310	39	goat	goat	NN
cord-288669-46tkedw7	310	40	anti	anti	JJ
cord-288669-46tkedw7	310	41	-	-	JJ
cord-288669-46tkedw7	310	42	mouse	mouse	JJ
cord-288669-46tkedw7	310	43	antibody	antibody	NN
cord-288669-46tkedw7	310	44	conjugated	conjugate	VBN
cord-288669-46tkedw7	310	45	with	with	IN
cord-288669-46tkedw7	310	46	Alexa	Alexa	NNP
cord-288669-46tkedw7	310	47	green	green	JJ
cord-288669-46tkedw7	310	48	.	.	.
cord-288669-46tkedw7	311	1	The	the	DT
cord-288669-46tkedw7	311	2	coverslips	coverslip	NNS
cord-288669-46tkedw7	311	3	were	be	VBD
cord-288669-46tkedw7	311	4	washed	wash	VBN
cord-288669-46tkedw7	311	5	five	five	CD
cord-288669-46tkedw7	311	6	times	time	NNS
cord-288669-46tkedw7	311	7	in	in	IN
cord-288669-46tkedw7	311	8	PBS	PBS	NNP
cord-288669-46tkedw7	311	9	and	and	CC
cord-288669-46tkedw7	311	10	mounted	mount	VBD
cord-288669-46tkedw7	311	11	on	on	IN
cord-288669-46tkedw7	311	12	microscope	microscope	NN
cord-288669-46tkedw7	311	13	glass	glass	NN
cord-288669-46tkedw7	311	14	slides	slide	NNS
cord-288669-46tkedw7	311	15	in	in	IN
cord-288669-46tkedw7	311	16	mounting	mount	VBG
cord-288669-46tkedw7	311	17	buffer	buffer	NN
cord-288669-46tkedw7	311	18	(	(	-LRB-
cord-288669-46tkedw7	311	19	60	60	CD
cord-288669-46tkedw7	311	20	%	%	NN
cord-288669-46tkedw7	311	21	glycerol	glycerol	NN
cord-288669-46tkedw7	311	22	and	and	CC
cord-288669-46tkedw7	311	23	0.1	0.1	CD
cord-288669-46tkedw7	311	24	%	%	NN
cord-288669-46tkedw7	311	25	sodium	sodium	NN
cord-288669-46tkedw7	311	26	azide	azide	NN
cord-288669-46tkedw7	311	27	in	in	IN
cord-288669-46tkedw7	311	28	PBS	PBS	NNP
cord-288669-46tkedw7	311	29	)	)	-RRB-
cord-288669-46tkedw7	311	30	.	.	.
cord-288669-46tkedw7	312	1	Cell	cell	NN
cord-288669-46tkedw7	312	2	staining	staining	NN
cord-288669-46tkedw7	312	3	was	be	VBD
cord-288669-46tkedw7	312	4	visualized	visualize	VBN
cord-288669-46tkedw7	312	5	using	use	VBG
cord-288669-46tkedw7	312	6	a	a	DT
cord-288669-46tkedw7	312	7	fluorescent	fluorescent	JJ
cord-288669-46tkedw7	312	8	microscope	microscope	NN
cord-288669-46tkedw7	312	9	(	(	-LRB-
cord-288669-46tkedw7	312	10	model	model	NN
cord-288669-46tkedw7	312	11	AX70	AX70	NNP
cord-288669-46tkedw7	312	12	;	;	:
cord-288669-46tkedw7	312	13	Olympus	Olympus	NNP
cord-288669-46tkedw7	312	14	)	)	-RRB-
cord-288669-46tkedw7	312	15	.	.	.
cord-288669-46tkedw7	313	1	BHK	BHK	NNP
cord-288669-46tkedw7	313	2	cells	cell	NNS
cord-288669-46tkedw7	313	3	were	be	VBD
cord-288669-46tkedw7	313	4	seeded	seed	VBN
cord-288669-46tkedw7	313	5	in	in	IN
cord-288669-46tkedw7	313	6	100	100	CD
cord-288669-46tkedw7	313	7	mm	mm	NN
cord-288669-46tkedw7	313	8	-	-	HYPH
cord-288669-46tkedw7	313	9	diameter	diameter	NN
cord-288669-46tkedw7	313	10	dishes	dish	NNS
cord-288669-46tkedw7	313	11	and	and	CC
cord-288669-46tkedw7	313	12	grown	grow	VBN
cord-288669-46tkedw7	313	13	to	to	IN
cord-288669-46tkedw7	313	14	70	70	CD
cord-288669-46tkedw7	313	15	%	%	NN
cord-288669-46tkedw7	313	16	confluence	confluence	NN
cord-288669-46tkedw7	313	17	.	.	.
cord-288669-46tkedw7	314	1	Cells	cell	NNS
cord-288669-46tkedw7	314	2	were	be	VBD
cord-288669-46tkedw7	314	3	transfected	transfecte	VBN
cord-288669-46tkedw7	314	4	for	for	IN
cord-288669-46tkedw7	314	5	20	20	CD
cord-288669-46tkedw7	314	6	h	h	NN
cord-288669-46tkedw7	314	7	with	with	IN
cord-288669-46tkedw7	314	8	10	10	CD
cord-288669-46tkedw7	314	9	μg	μg	NNS
cord-288669-46tkedw7	314	10	of	of	IN
cord-288669-46tkedw7	314	11	the	the	DT
cord-288669-46tkedw7	314	12	full	full	JJ
cord-288669-46tkedw7	314	13	-	-	HYPH
cord-288669-46tkedw7	314	14	length	length	NN
cord-288669-46tkedw7	314	15	cDNA	cdna	NN
cord-288669-46tkedw7	314	16	plasmid	plasmid	NN
cord-288669-46tkedw7	314	17	using	use	VBG
cord-288669-46tkedw7	314	18	Lipofectin	Lipofectin	NNP
cord-288669-46tkedw7	314	19	.	.	.
cord-288669-46tkedw7	315	1	The	the	DT
cord-288669-46tkedw7	315	2	transfected	transfecte	VBN
cord-288669-46tkedw7	315	3	cells	cell	NNS
cord-288669-46tkedw7	315	4	were	be	VBD
cord-288669-46tkedw7	315	5	continued	continue	VBN
cord-288669-46tkedw7	315	6	for	for	IN
cord-288669-46tkedw7	315	7	incubation	incubation	NN
cord-288669-46tkedw7	315	8	at	at	IN
cord-288669-46tkedw7	315	9	37	37	CD
cord-288669-46tkedw7	315	10	°	°	NN
cord-288669-46tkedw7	315	11	C	c	NN
cord-288669-46tkedw7	315	12	in	in	IN
cord-288669-46tkedw7	315	13	DMEM	DMEM	NNP
cord-288669-46tkedw7	315	14	supplemented	supplement	VBD
cord-288669-46tkedw7	315	15	with	with	IN
cord-288669-46tkedw7	315	16	8	8	CD
cord-288669-46tkedw7	315	17	%	%	NN
cord-288669-46tkedw7	315	18	FBS	FBS	NNP
cord-288669-46tkedw7	315	19	for	for	IN
cord-288669-46tkedw7	315	20	10	10	CD
cord-288669-46tkedw7	315	21	h.	h.	NN
cord-288669-46tkedw7	316	1	At	at	IN
cord-288669-46tkedw7	316	2	30	30	CD
cord-288669-46tkedw7	316	3	h	h	NN
cord-288669-46tkedw7	316	4	posttransfection	posttransfection	NN
cord-288669-46tkedw7	316	5	,	,	,
cord-288669-46tkedw7	316	6	the	the	DT
cord-288669-46tkedw7	316	7	cells	cell	NNS
cord-288669-46tkedw7	316	8	were	be	VBD
cord-288669-46tkedw7	316	9	starved	starve	VBN
cord-288669-46tkedw7	316	10	for	for	IN
cord-288669-46tkedw7	316	11	30	30	CD
cord-288669-46tkedw7	316	12	min	min	NN
cord-288669-46tkedw7	316	13	in	in	IN
cord-288669-46tkedw7	316	14	methioninedeficient	methioninedeficient	NN
cord-288669-46tkedw7	316	15	MEM	MEM	NNP
cord-288669-46tkedw7	316	16	(	(	-LRB-
cord-288669-46tkedw7	316	17	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	316	18	)	)	-RRB-
cord-288669-46tkedw7	316	19	and	and	CC
cord-288669-46tkedw7	316	20	metabolically	metabolically	RB
cord-288669-46tkedw7	316	21	labeled	label	VBN
cord-288669-46tkedw7	316	22	for	for	IN
cord-288669-46tkedw7	316	23	24	24	CD
cord-288669-46tkedw7	316	24	h	h	NN
cord-288669-46tkedw7	316	25	with	with	IN
cord-288669-46tkedw7	316	26	100	100	CD
cord-288669-46tkedw7	316	27	μCi	μCi	NNS
cord-288669-46tkedw7	316	28	/	/	SYM
cord-288669-46tkedw7	316	29	ml	ml	NNS
cord-288669-46tkedw7	316	30	of	of	IN
cord-288669-46tkedw7	316	31	EasyTag	EasyTag	NNP
cord-288669-46tkedw7	316	32	EXPRESS	EXPRESS	NNP
cord-288669-46tkedw7	316	33	protein	protein	NN
cord-288669-46tkedw7	316	34	labeling	labeling	NN
cord-288669-46tkedw7	316	35	mix	mix	NN
cord-288669-46tkedw7	316	36	(	(	-LRB-
cord-288669-46tkedw7	316	37	[	[	-LRB-
cord-288669-46tkedw7	316	38	35	35	CD
cord-288669-46tkedw7	316	39	S]methionine	S]methionine	NNP
cord-288669-46tkedw7	316	40	and	and	CC
cord-288669-46tkedw7	316	41	[	[	-LRB-
cord-288669-46tkedw7	316	42	35	35	CD
cord-288669-46tkedw7	316	43	S]cysteine	S]cysteine	NNP
cord-288669-46tkedw7	316	44	,	,	,
cord-288669-46tkedw7	316	45	specific	specific	JJ
cord-288669-46tkedw7	316	46	activity	activity	NN
cord-288669-46tkedw7	316	47	,	,	,
cord-288669-46tkedw7	316	48	407	407	CD
cord-288669-46tkedw7	316	49	MBq/	MBq/	NNPS
cord-288669-46tkedw7	316	50	ml	ml	NN
cord-288669-46tkedw7	316	51	)	)	-RRB-
cord-288669-46tkedw7	317	1	(	(	-LRB-
cord-288669-46tkedw7	317	2	Perkin	Perkin	NNP
cord-288669-46tkedw7	317	3	-	-	HYPH
cord-288669-46tkedw7	317	4	Elmer	Elmer	NNP
cord-288669-46tkedw7	317	5	)	)	-RRB-
cord-288669-46tkedw7	317	6	.	.	.
cord-288669-46tkedw7	318	1	After	after	IN
cord-288669-46tkedw7	318	2	a	a	DT
cord-288669-46tkedw7	318	3	1-day	1-day	JJ
cord-288669-46tkedw7	318	4	labeling	labeling	NN
cord-288669-46tkedw7	318	5	period	period	NN
cord-288669-46tkedw7	318	6	,	,	,
cord-288669-46tkedw7	318	7	the	the	DT
cord-288669-46tkedw7	318	8	cells	cell	NNS
cord-288669-46tkedw7	318	9	were	be	VBD
cord-288669-46tkedw7	318	10	washed	wash	VBN
cord-288669-46tkedw7	318	11	twice	twice	RB
cord-288669-46tkedw7	318	12	with	with	IN
cord-288669-46tkedw7	318	13	cold	cold	JJ
cord-288669-46tkedw7	318	14	PBS	PBS	NNP
cord-288669-46tkedw7	318	15	and	and	CC
cord-288669-46tkedw7	318	16	lysed	lyse	VBN
cord-288669-46tkedw7	318	17	with	with	IN
cord-288669-46tkedw7	318	18	RIPA	RIPA	NNP
cord-288669-46tkedw7	318	19	buffer	buffer	NN
cord-288669-46tkedw7	318	20	(	(	-LRB-
cord-288669-46tkedw7	318	21	1	1	CD
cord-288669-46tkedw7	318	22	%	%	NN
cord-288669-46tkedw7	318	23	Triton	Triton	NNP
cord-288669-46tkedw7	318	24	X-100	x-100	NN
cord-288669-46tkedw7	318	25	,	,	,
cord-288669-46tkedw7	318	26	1	1	CD
cord-288669-46tkedw7	318	27	%	%	NN
cord-288669-46tkedw7	318	28	sodium	sodium	NN
cord-288669-46tkedw7	318	29	deoxycholate	deoxycholate	NN
cord-288669-46tkedw7	318	30	,	,	,
cord-288669-46tkedw7	318	31	150	150	CD
cord-288669-46tkedw7	318	32	mM	mm	CD
cord-288669-46tkedw7	318	33	NaCl	nacl	NN
cord-288669-46tkedw7	318	34	,	,	,
cord-288669-46tkedw7	318	35	50	50	CD
cord-288669-46tkedw7	318	36	mM	mm	CD
cord-288669-46tkedw7	318	37	Tris	Tris	NNP
cord-288669-46tkedw7	318	38	-	-	HYPH
cord-288669-46tkedw7	318	39	HCl	HCl	NNP
cord-288669-46tkedw7	318	40	[	[	-LRB-
cord-288669-46tkedw7	318	41	pH	pH	NNP
cord-288669-46tkedw7	318	42	7.4	7.4	CD
cord-288669-46tkedw7	318	43	]	]	-RRB-
cord-288669-46tkedw7	318	44	,	,	,
cord-288669-46tkedw7	318	45	10	10	CD
cord-288669-46tkedw7	318	46	mM	mm	CD
cord-288669-46tkedw7	318	47	EDTA	EDTA	NNP
cord-288669-46tkedw7	318	48	,	,	,
cord-288669-46tkedw7	318	49	0.1	0.1	CD
cord-288669-46tkedw7	318	50	%	%	NN
cord-288669-46tkedw7	318	51	SDS	SDS	NNP
cord-288669-46tkedw7	318	52	)	)	-RRB-
cord-288669-46tkedw7	318	53	containing	contain	VBG
cord-288669-46tkedw7	318	54	1	1	CD
cord-288669-46tkedw7	318	55	mM	mm	CD
cord-288669-46tkedw7	318	56	phenylmethylsulfonyl	phenylmethylsulfonyl	NN
cord-288669-46tkedw7	318	57	fluoride	fluoride	NN
cord-288669-46tkedw7	318	58	(	(	-LRB-
cord-288669-46tkedw7	318	59	PMSF	PMSF	NNP
cord-288669-46tkedw7	318	60	)	)	-RRB-
cord-288669-46tkedw7	318	61	.	.	.
cord-288669-46tkedw7	319	1	To	to	TO
cord-288669-46tkedw7	319	2	prepare	prepare	VB
cord-288669-46tkedw7	319	3	radiolabeled	radiolabele	VBN
cord-288669-46tkedw7	319	4	viral	viral	JJ
cord-288669-46tkedw7	319	5	particle	particle	NN
cord-288669-46tkedw7	319	6	samples	sample	NNS
cord-288669-46tkedw7	319	7	,	,	,
cord-288669-46tkedw7	319	8	the	the	DT
cord-288669-46tkedw7	319	9	culture	culture	NN
cord-288669-46tkedw7	319	10	supernatant	supernatant	JJ
cord-288669-46tkedw7	319	11	was	be	VBD
cord-288669-46tkedw7	319	12	harvested	harvest	VBN
cord-288669-46tkedw7	319	13	,	,	,
cord-288669-46tkedw7	319	14	and	and	CC
cord-288669-46tkedw7	319	15	the	the	DT
cord-288669-46tkedw7	319	16	cell	cell	NN
cord-288669-46tkedw7	319	17	debris	debris	NN
cord-288669-46tkedw7	319	18	was	be	VBD
cord-288669-46tkedw7	319	19	removed	remove	VBN
cord-288669-46tkedw7	319	20	by	by	IN
cord-288669-46tkedw7	319	21	a	a	DT
cord-288669-46tkedw7	319	22	low	low	JJ
cord-288669-46tkedw7	319	23	-	-	HYPH
cord-288669-46tkedw7	319	24	speed	speed	NN
cord-288669-46tkedw7	319	25	centrifugation	centrifugation	NN
cord-288669-46tkedw7	319	26	at	at	IN
cord-288669-46tkedw7	319	27	3500	3500	CD
cord-288669-46tkedw7	319	28	rpm	rpm	NN
cord-288669-46tkedw7	319	29	(	(	-LRB-
cord-288669-46tkedw7	319	30	model	model	NN
cord-288669-46tkedw7	319	31	5415	5415	CD
cord-288669-46tkedw7	319	32	;	;	:
cord-288669-46tkedw7	319	33	Eppendorf	Eppendorf	NNP
cord-288669-46tkedw7	319	34	)	)	-RRB-
cord-288669-46tkedw7	319	35	for	for	IN
cord-288669-46tkedw7	319	36	10	10	CD
cord-288669-46tkedw7	319	37	min	min	NN
cord-288669-46tkedw7	319	38	at	at	IN
cord-288669-46tkedw7	319	39	RT	RT	NNP
cord-288669-46tkedw7	319	40	.	.	.
cord-288669-46tkedw7	320	1	The	the	DT
cord-288669-46tkedw7	320	2	virus	virus	NN
cord-288669-46tkedw7	320	3	particles	particle	NNS
cord-288669-46tkedw7	320	4	were	be	VBD
cord-288669-46tkedw7	320	5	purified	purify	VBN
cord-288669-46tkedw7	320	6	through	through	IN
cord-288669-46tkedw7	320	7	a	a	DT
cord-288669-46tkedw7	320	8	20	20	CD
cord-288669-46tkedw7	320	9	%	%	NN
cord-288669-46tkedw7	320	10	sucrose	sucrose	NN
cord-288669-46tkedw7	320	11	cushion	cushion	NN
cord-288669-46tkedw7	320	12	(	(	-LRB-
cord-288669-46tkedw7	320	13	wt	wt	NN
cord-288669-46tkedw7	320	14	/	/	SYM
cord-288669-46tkedw7	320	15	vol	vol	NN
cord-288669-46tkedw7	320	16	)	)	-RRB-
cord-288669-46tkedw7	320	17	prepared	prepare	VBN
cord-288669-46tkedw7	320	18	in	in	IN
cord-288669-46tkedw7	320	19	TE	TE	NNP
cord-288669-46tkedw7	320	20	buffer	buffer	NN
cord-288669-46tkedw7	320	21	(	(	-LRB-
cord-288669-46tkedw7	320	22	10	10	CD
cord-288669-46tkedw7	320	23	mM	mm	CD
cord-288669-46tkedw7	320	24	Tris	Tris	NNP
cord-288669-46tkedw7	320	25	HCl	hcl	NN
cord-288669-46tkedw7	320	26	[	[	-LRB-
cord-288669-46tkedw7	320	27	pH	pH	NNP
cord-288669-46tkedw7	320	28	8	8	CD
cord-288669-46tkedw7	320	29	]	]	-RRB-
cord-288669-46tkedw7	320	30	,	,	,
cord-288669-46tkedw7	320	31	1	1	CD
cord-288669-46tkedw7	320	32	mM	mm	CD
cord-288669-46tkedw7	320	33	EDTA	EDTA	NNP
cord-288669-46tkedw7	320	34	)	)	-RRB-
cord-288669-46tkedw7	320	35	at	at	IN
cord-288669-46tkedw7	320	36	40,000	40,000	CD
cord-288669-46tkedw7	320	37	rpm	rpm	NN
cord-288669-46tkedw7	320	38	for	for	IN
cord-288669-46tkedw7	320	39	2	2	CD
cord-288669-46tkedw7	320	40	h	h	NN
cord-288669-46tkedw7	320	41	at	at	IN
cord-288669-46tkedw7	320	42	4	4	CD
cord-288669-46tkedw7	320	43	°	°	NN
cord-288669-46tkedw7	320	44	C	c	NN
cord-288669-46tkedw7	320	45	in	in	IN
cord-288669-46tkedw7	320	46	an	an	DT
cord-288669-46tkedw7	320	47	SW41	SW41	NNP
cord-288669-46tkedw7	320	48	rotor	rotor	NN
cord-288669-46tkedw7	320	49	(	(	-LRB-
cord-288669-46tkedw7	320	50	model	model	NN
cord-288669-46tkedw7	320	51	XL-90	XL-90	NNP
cord-288669-46tkedw7	320	52	;	;	:
cord-288669-46tkedw7	320	53	Beckman	Beckman	NNP
cord-288669-46tkedw7	320	54	)	)	-RRB-
cord-288669-46tkedw7	320	55	.	.	.
cord-288669-46tkedw7	321	1	The	the	DT
cord-288669-46tkedw7	321	2	resulting	result	VBG
cord-288669-46tkedw7	321	3	pellets	pellet	NNS
cord-288669-46tkedw7	321	4	were	be	VBD
cord-288669-46tkedw7	321	5	resuspended	resuspend	VBN
cord-288669-46tkedw7	321	6	in	in	IN
cord-288669-46tkedw7	321	7	50	50	CD
cord-288669-46tkedw7	321	8	μl	μl	NN
cord-288669-46tkedw7	321	9	of	of	IN
cord-288669-46tkedw7	321	10	RIPA	RIPA	NNP
cord-288669-46tkedw7	321	11	buffer	buffer	NN
cord-288669-46tkedw7	321	12	containing	contain	VBG
cord-288669-46tkedw7	321	13	1	1	CD
cord-288669-46tkedw7	321	14	mM	mm	CD
cord-288669-46tkedw7	321	15	PMSF	PMSF	NNP
cord-288669-46tkedw7	321	16	.	.	.
cord-288669-46tkedw7	322	1	For	for	IN
cord-288669-46tkedw7	322	2	immunoprecipitation	immunoprecipitation	NN
cord-288669-46tkedw7	322	3	,	,	,
cord-288669-46tkedw7	322	4	the	the	DT
cord-288669-46tkedw7	322	5	dissolved	dissolve	VBN
cord-288669-46tkedw7	322	6	pellets	pellet	NNS
cord-288669-46tkedw7	322	7	or	or	CC
cord-288669-46tkedw7	322	8	cell	cell	NN
cord-288669-46tkedw7	322	9	lysates	lysate	NNS
cord-288669-46tkedw7	322	10	equivalent	equivalent	JJ
cord-288669-46tkedw7	322	11	to	to	IN
cord-288669-46tkedw7	322	12	1	1	CD
cord-288669-46tkedw7	322	13	in	in	IN
cord-288669-46tkedw7	322	14	15	15	CD
cord-288669-46tkedw7	322	15	of	of	IN
cord-288669-46tkedw7	322	16	a	a	DT
cord-288669-46tkedw7	322	17	100-mm	100-mm	CD
cord-288669-46tkedw7	322	18	-	-	HYPH
cord-288669-46tkedw7	322	19	diameter	diameter	NN
cord-288669-46tkedw7	322	20	dish	dish	NN
cord-288669-46tkedw7	322	21	were	be	VBD
cord-288669-46tkedw7	322	22	adjusted	adjust	VBN
cord-288669-46tkedw7	322	23	with	with	IN
cord-288669-46tkedw7	322	24	RIPA	RIPA	NNP
cord-288669-46tkedw7	322	25	buffer	buffer	NN
cord-288669-46tkedw7	322	26	to	to	IN
cord-288669-46tkedw7	322	27	a	a	DT
cord-288669-46tkedw7	322	28	final	final	JJ
cord-288669-46tkedw7	322	29	volume	volume	NN
cord-288669-46tkedw7	322	30	of	of	IN
cord-288669-46tkedw7	322	31	100	100	CD
cord-288669-46tkedw7	322	32	μl	μl	NNS
cord-288669-46tkedw7	322	33	and	and	CC
cord-288669-46tkedw7	322	34	incubated	incubate	VBN
cord-288669-46tkedw7	322	35	for	for	IN
cord-288669-46tkedw7	322	36	2	2	CD
cord-288669-46tkedw7	322	37	h	h	NN
cord-288669-46tkedw7	322	38	at	at	IN
cord-288669-46tkedw7	322	39	RT	RT	NNP
cord-288669-46tkedw7	322	40	with	with	IN
cord-288669-46tkedw7	322	41	a	a	DT
cord-288669-46tkedw7	322	42	mixture	mixture	NN
cord-288669-46tkedw7	322	43	of	of	IN
cord-288669-46tkedw7	322	44	E	e	NN
cord-288669-46tkedw7	322	45	-	-	JJ
cord-288669-46tkedw7	322	46	specific	specific	JJ
cord-288669-46tkedw7	322	47	rabbit	rabbit	NN
cord-288669-46tkedw7	322	48	antiserum	antiserum	NN
cord-288669-46tkedw7	322	49	,	,	,
cord-288669-46tkedw7	323	1	Nspecific	Nspecific	NNP
cord-288669-46tkedw7	323	2	MAb	MAb	NNS
cord-288669-46tkedw7	324	1	SDOW17	sdow17	XX
cord-288669-46tkedw7	324	2	,	,	,
cord-288669-46tkedw7	324	3	and	and	CC
cord-288669-46tkedw7	324	4	M	M	NNP
cord-288669-46tkedw7	324	5	-	-	HYPH
cord-288669-46tkedw7	324	6	specific	specific	JJ
cord-288669-46tkedw7	324	7	rabbit	rabbit	NN
cord-288669-46tkedw7	324	8	antiserum	antiserum	NN
cord-288669-46tkedw7	324	9	.	.	.
cord-288669-46tkedw7	325	1	The	the	DT
cord-288669-46tkedw7	325	2	immune	immune	JJ
cord-288669-46tkedw7	325	3	complexes	complex	NNS
cord-288669-46tkedw7	325	4	were	be	VBD
cord-288669-46tkedw7	325	5	adsorbed	adsorb	VBN
cord-288669-46tkedw7	325	6	to	to	IN
cord-288669-46tkedw7	325	7	7	7	CD
cord-288669-46tkedw7	325	8	mg	mg	NN
cord-288669-46tkedw7	325	9	of	of	IN
cord-288669-46tkedw7	325	10	protein	protein	NN
cord-288669-46tkedw7	325	11	-	-	:
cord-288669-46tkedw7	325	12	A	a	DT
cord-288669-46tkedw7	325	13	Sepharose	Sepharose	NNP
cord-288669-46tkedw7	325	14	CL-4B	CL-4B	NNP
cord-288669-46tkedw7	325	15	beads	bead	NNS
cord-288669-46tkedw7	325	16	(	(	-LRB-
cord-288669-46tkedw7	325	17	Amersham	Amersham	NNP
cord-288669-46tkedw7	325	18	Biosciences	Biosciences	NNP
cord-288669-46tkedw7	325	19	)	)	-RRB-
cord-288669-46tkedw7	325	20	for	for	IN
cord-288669-46tkedw7	325	21	16	16	CD
cord-288669-46tkedw7	325	22	h	h	NN
cord-288669-46tkedw7	325	23	at	at	IN
cord-288669-46tkedw7	325	24	4	4	CD
cord-288669-46tkedw7	325	25	°	°	NN
cord-288669-46tkedw7	325	26	C	c	NN
cord-288669-46tkedw7	325	27	.	.	.
cord-288669-46tkedw7	326	1	The	the	DT
cord-288669-46tkedw7	326	2	beads	bead	NNS
cord-288669-46tkedw7	326	3	were	be	VBD
cord-288669-46tkedw7	326	4	collected	collect	VBN
cord-288669-46tkedw7	326	5	by	by	IN
cord-288669-46tkedw7	326	6	centrifugation	centrifugation	NN
cord-288669-46tkedw7	326	7	at	at	IN
cord-288669-46tkedw7	326	8	6000	6000	CD
cord-288669-46tkedw7	326	9	rpm	rpm	NN
cord-288669-46tkedw7	326	10	for	for	IN
cord-288669-46tkedw7	326	11	5	5	CD
cord-288669-46tkedw7	326	12	min	min	NN
cord-288669-46tkedw7	326	13	,	,	,
cord-288669-46tkedw7	326	14	washed	wash	VBN
cord-288669-46tkedw7	326	15	twice	twice	RB
cord-288669-46tkedw7	326	16	with	with	IN
cord-288669-46tkedw7	326	17	RIPA	RIPA	NNP
cord-288669-46tkedw7	326	18	buffer	buffer	NN
cord-288669-46tkedw7	326	19	and	and	CC
cord-288669-46tkedw7	326	20	once	once	RB
cord-288669-46tkedw7	326	21	with	with	IN
cord-288669-46tkedw7	326	22	wash	wash	NN
cord-288669-46tkedw7	326	23	buffer	buffer	NN
cord-288669-46tkedw7	326	24	(	(	-LRB-
cord-288669-46tkedw7	326	25	50	50	CD
cord-288669-46tkedw7	326	26	mM	mM	NNP
cord-288669-46tkedw7	326	27	Tris	Tris	NNP
cord-288669-46tkedw7	326	28	-	-	HYPH
cord-288669-46tkedw7	326	29	HCl	HCl	NNP
cord-288669-46tkedw7	326	30	[	[	-LRB-
cord-288669-46tkedw7	326	31	pH	pH	NNP
cord-288669-46tkedw7	326	32	7.4	7.4	CD
cord-288669-46tkedw7	326	33	]	]	-RRB-
cord-288669-46tkedw7	326	34	,	,	,
cord-288669-46tkedw7	326	35	150	150	CD
cord-288669-46tkedw7	326	36	mM	mm	CD
cord-288669-46tkedw7	326	37	NaCl	nacl	NN
cord-288669-46tkedw7	326	38	)	)	-RRB-
cord-288669-46tkedw7	326	39	.	.	.
cord-288669-46tkedw7	327	1	The	the	DT
cord-288669-46tkedw7	327	2	beads	bead	NNS
cord-288669-46tkedw7	327	3	were	be	VBD
cord-288669-46tkedw7	327	4	resuspended	resuspend	VBN
cord-288669-46tkedw7	327	5	in	in	IN
cord-288669-46tkedw7	327	6	20	20	CD
cord-288669-46tkedw7	327	7	μl	μl	NN
cord-288669-46tkedw7	327	8	of	of	IN
cord-288669-46tkedw7	327	9	SDS	SDS	NNP
cord-288669-46tkedw7	327	10	-	-	HYPH
cord-288669-46tkedw7	327	11	PAGE	page	NN
cord-288669-46tkedw7	327	12	sample	sample	NN
cord-288669-46tkedw7	327	13	buffer	buffer	NN
cord-288669-46tkedw7	327	14	(	(	-LRB-
cord-288669-46tkedw7	327	15	10	10	CD
cord-288669-46tkedw7	327	16	mM	mM	NNP
cord-288669-46tkedw7	327	17	Tris	Tris	NNP
cord-288669-46tkedw7	327	18	-	-	HYPH
cord-288669-46tkedw7	327	19	HCl	HCl	NNP
cord-288669-46tkedw7	327	20	[	[	-LRB-
cord-288669-46tkedw7	327	21	pH	pH	NNP
cord-288669-46tkedw7	327	22	6.8	6.8	CD
cord-288669-46tkedw7	327	23	]	]	-RRB-
cord-288669-46tkedw7	327	24	,	,	,
cord-288669-46tkedw7	327	25	25	25	CD
cord-288669-46tkedw7	327	26	%	%	NN
cord-288669-46tkedw7	327	27	glycerol	glycerol	NN
cord-288669-46tkedw7	327	28	,	,	,
cord-288669-46tkedw7	327	29	10	10	CD
cord-288669-46tkedw7	327	30	%	%	NN
cord-288669-46tkedw7	327	31	SDS	SDS	NNP
cord-288669-46tkedw7	327	32	,	,	,
cord-288669-46tkedw7	327	33	0.12	0.12	CD
cord-288669-46tkedw7	327	34	%	%	NN
cord-288669-46tkedw7	327	35	[	[	-LRB-
cord-288669-46tkedw7	327	36	wt	wt	NN
cord-288669-46tkedw7	327	37	/	/	SYM
cord-288669-46tkedw7	327	38	vol	vol	NNP
cord-288669-46tkedw7	327	39	]	]	-RRB-
cord-288669-46tkedw7	327	40	bromophenol	bromophenol	NNP
cord-288669-46tkedw7	327	41	blue	blue	NNP
cord-288669-46tkedw7	327	42	)	)	-RRB-
cord-288669-46tkedw7	327	43	with	with	IN
cord-288669-46tkedw7	327	44	10	10	CD
cord-288669-46tkedw7	327	45	%	%	NN
cord-288669-46tkedw7	327	46	β	β	NN
cord-288669-46tkedw7	327	47	-	-	HYPH
cord-288669-46tkedw7	327	48	mercaptoethanol	mercaptoethanol	NNP
cord-288669-46tkedw7	327	49	(	(	-LRB-
cord-288669-46tkedw7	327	50	βME	βME	NNP
cord-288669-46tkedw7	327	51	)	)	-RRB-
cord-288669-46tkedw7	327	52	,	,	,
cord-288669-46tkedw7	327	53	boiled	boil	VBN
cord-288669-46tkedw7	327	54	for	for	IN
cord-288669-46tkedw7	327	55	5	5	CD
cord-288669-46tkedw7	327	56	min	min	NN
cord-288669-46tkedw7	327	57	and	and	CC
cord-288669-46tkedw7	327	58	analyzed	analyze	VBN
cord-288669-46tkedw7	327	59	by	by	IN
cord-288669-46tkedw7	327	60	15	15	CD
cord-288669-46tkedw7	327	61	%	%	NN
cord-288669-46tkedw7	328	1	SDSpolyacrylamide	SDSpolyacrylamide	NNP
cord-288669-46tkedw7	328	2	gel	gel	NN
cord-288669-46tkedw7	328	3	electrophoresis	electrophoresis	NN
cord-288669-46tkedw7	328	4	(	(	-LRB-
cord-288669-46tkedw7	328	5	PAGE	page	NN
cord-288669-46tkedw7	328	6	)	)	-RRB-
cord-288669-46tkedw7	328	7	.	.	.
cord-288669-46tkedw7	329	1	Gels	gel	NNS
cord-288669-46tkedw7	329	2	were	be	VBD
cord-288669-46tkedw7	329	3	dried	dry	VBN
cord-288669-46tkedw7	329	4	on	on	IN
cord-288669-46tkedw7	329	5	filter	filter	NN
cord-288669-46tkedw7	329	6	paper	paper	NN
cord-288669-46tkedw7	329	7	and	and	CC
cord-288669-46tkedw7	329	8	radiographic	radiographic	JJ
cord-288669-46tkedw7	329	9	images	image	NNS
cord-288669-46tkedw7	329	10	were	be	VBD
cord-288669-46tkedw7	329	11	obtained	obtain	VBN
cord-288669-46tkedw7	329	12	using	use	VBG
cord-288669-46tkedw7	329	13	a	a	DT
cord-288669-46tkedw7	329	14	phosphorimager	phosphorimager	NN
cord-288669-46tkedw7	329	15	(	(	-LRB-
cord-288669-46tkedw7	329	16	model	model	NN
cord-288669-46tkedw7	329	17	PhosphorImager	PhosphorImager	NNP
cord-288669-46tkedw7	329	18	SI	SI	NNP
cord-288669-46tkedw7	329	19	;	;	:
cord-288669-46tkedw7	329	20	Molecular	Molecular	NNP
cord-288669-46tkedw7	329	21	Dynamics	Dynamics	NNPS
cord-288669-46tkedw7	329	22	)	)	-RRB-
cord-288669-46tkedw7	329	23	.	.	.
cord-288669-46tkedw7	330	1	Virus	virus	NN
cord-288669-46tkedw7	330	2	preparation	preparation	NN
cord-288669-46tkedw7	330	3	from	from	IN
cord-288669-46tkedw7	330	4	full	full	JJ
cord-288669-46tkedw7	330	5	-	-	HYPH
cord-288669-46tkedw7	330	6	length	length	NN
cord-288669-46tkedw7	330	7	cDNA	cdna	NN
cord-288669-46tkedw7	330	8	clones	clone	NNS
cord-288669-46tkedw7	330	9	BHK-21	bhk-21	IN
cord-288669-46tkedw7	330	10	cells	cell	NNS
cord-288669-46tkedw7	330	11	were	be	VBD
cord-288669-46tkedw7	330	12	transfected	transfecte	VBN
cord-288669-46tkedw7	330	13	with	with	IN
cord-288669-46tkedw7	330	14	the	the	DT
cord-288669-46tkedw7	330	15	full	full	JJ
cord-288669-46tkedw7	330	16	-	-	HYPH
cord-288669-46tkedw7	330	17	length	length	NN
cord-288669-46tkedw7	330	18	cDNA	cdna	NN
cord-288669-46tkedw7	330	19	plasmid	plasmid	NN
cord-288669-46tkedw7	330	20	as	as	IN
cord-288669-46tkedw7	330	21	described	describe	VBN
cord-288669-46tkedw7	330	22	above	above	RB
cord-288669-46tkedw7	330	23	.	.	.
cord-288669-46tkedw7	331	1	After	after	IN
cord-288669-46tkedw7	331	2	washing	washing	NN
cord-288669-46tkedw7	331	3	of	of	IN
cord-288669-46tkedw7	331	4	cells	cell	NNS
cord-288669-46tkedw7	331	5	with	with	IN
cord-288669-46tkedw7	331	6	DMEM	DMEM	NNP
cord-288669-46tkedw7	331	7	at	at	IN
cord-288669-46tkedw7	331	8	20	20	CD
cord-288669-46tkedw7	331	9	h	h	NN
cord-288669-46tkedw7	331	10	post	post	NN
cord-288669-46tkedw7	331	11	-	-	NN
cord-288669-46tkedw7	331	12	transfection	transfection	JJ
cord-288669-46tkedw7	331	13	,	,	,
cord-288669-46tkedw7	331	14	the	the	DT
cord-288669-46tkedw7	331	15	transfected	transfecte	VBN
cord-288669-46tkedw7	331	16	cells	cell	NNS
cord-288669-46tkedw7	331	17	were	be	VBD
cord-288669-46tkedw7	331	18	further	further	RB
cord-288669-46tkedw7	331	19	maintained	maintain	VBN
cord-288669-46tkedw7	331	20	in	in	IN
cord-288669-46tkedw7	331	21	DMEM	DMEM	NNP
cord-288669-46tkedw7	331	22	supplemented	supplement	VBD
cord-288669-46tkedw7	331	23	with	with	IN
cord-288669-46tkedw7	331	24	8	8	CD
cord-288669-46tkedw7	331	25	%	%	NN
cord-288669-46tkedw7	331	26	FBS	fbs	NN
cord-288669-46tkedw7	331	27	for	for	IN
cord-288669-46tkedw7	331	28	2	2	CD
cord-288669-46tkedw7	331	29	days	day	NNS
cord-288669-46tkedw7	331	30	and	and	CC
cord-288669-46tkedw7	331	31	the	the	DT
cord-288669-46tkedw7	331	32	culture	culture	NN
cord-288669-46tkedw7	331	33	supernatants	supernatant	NNS
cord-288669-46tkedw7	331	34	were	be	VBD
cord-288669-46tkedw7	331	35	harvested	harvest	VBN
cord-288669-46tkedw7	331	36	at	at	IN
cord-288669-46tkedw7	331	37	2	2	CD
cord-288669-46tkedw7	331	38	days	day	NNS
cord-288669-46tkedw7	331	39	posttransfection	posttransfection	NN
cord-288669-46tkedw7	331	40	.	.	.
cord-288669-46tkedw7	332	1	The	the	DT
cord-288669-46tkedw7	332	2	viral	viral	JJ
cord-288669-46tkedw7	332	3	particles	particle	NNS
cord-288669-46tkedw7	332	4	were	be	VBD
cord-288669-46tkedw7	332	5	purified	purify	VBN
cord-288669-46tkedw7	332	6	as	as	IN
cord-288669-46tkedw7	332	7	described	describe	VBN
cord-288669-46tkedw7	332	8	above	above	IN
cord-288669-46tkedw7	332	9	and	and	CC
cord-288669-46tkedw7	332	10	the	the	DT
cord-288669-46tkedw7	332	11	pellets	pellet	NNS
cord-288669-46tkedw7	332	12	were	be	VBD
cord-288669-46tkedw7	332	13	suspended	suspend	VBN
cord-288669-46tkedw7	332	14	in	in	IN
cord-288669-46tkedw7	332	15	DMEM	DMEM	NNP
cord-288669-46tkedw7	332	16	.	.	.
cord-288669-46tkedw7	333	1	The	the	DT
cord-288669-46tkedw7	333	2	resulting	result	VBG
cord-288669-46tkedw7	333	3	virus	virus	NN
cord-288669-46tkedw7	333	4	suspension	suspension	NN
cord-288669-46tkedw7	333	5	was	be	VBD
cord-288669-46tkedw7	333	6	designated	designate	VBN
cord-288669-46tkedw7	333	7	'	'	``
cord-288669-46tkedw7	333	8	passage-1	passage-1	NNP
cord-288669-46tkedw7	333	9	'	'	''
cord-288669-46tkedw7	333	10	.	.	.
cord-288669-46tkedw7	334	1	The	the	DT
cord-288669-46tkedw7	334	2	passage-1	passage-1	NNP
cord-288669-46tkedw7	334	3	virus	virus	NN
cord-288669-46tkedw7	334	4	was	be	VBD
cord-288669-46tkedw7	334	5	used	use	VBN
cord-288669-46tkedw7	334	6	to	to	TO
cord-288669-46tkedw7	334	7	inoculate	inoculate	VB
cord-288669-46tkedw7	334	8	fresh	fresh	JJ
cord-288669-46tkedw7	334	9	Marc-145	marc-145	CD
cord-288669-46tkedw7	334	10	cells	cell	NNS
cord-288669-46tkedw7	334	11	and	and	CC
cord-288669-46tkedw7	334	12	the	the	DT
cord-288669-46tkedw7	334	13	5-day	5-day	JJ
cord-288669-46tkedw7	334	14	harvest	harvest	NN
cord-288669-46tkedw7	334	15	was	be	VBD
cord-288669-46tkedw7	334	16	designated	designate	VBN
cord-288669-46tkedw7	334	17	'	'	``
cord-288669-46tkedw7	334	18	passage-2	passage-2	NNP
cord-288669-46tkedw7	334	19	'	'	''
cord-288669-46tkedw7	334	20	.	.	.
cord-288669-46tkedw7	335	1	The	the	DT
cord-288669-46tkedw7	335	2	'	'	``
cord-288669-46tkedw7	335	3	passage-3	passage-3	NNP
cord-288669-46tkedw7	335	4	'	'	POS
cord-288669-46tkedw7	335	5	virus	virus	NN
cord-288669-46tkedw7	335	6	was	be	VBD
cord-288669-46tkedw7	335	7	prepared	prepare	VBN
cord-288669-46tkedw7	335	8	in	in	IN
cord-288669-46tkedw7	335	9	the	the	DT
cord-288669-46tkedw7	335	10	same	same	JJ
cord-288669-46tkedw7	335	11	way	way	NN
cord-288669-46tkedw7	335	12	as	as	IN
cord-288669-46tkedw7	335	13	for	for	IN
cord-288669-46tkedw7	335	14	passage-2	passage-2	NNP
cord-288669-46tkedw7	335	15	.	.	.
cord-288669-46tkedw7	336	1	Each	each	DT
cord-288669-46tkedw7	336	2	passage	passage	NN
cord-288669-46tkedw7	336	3	virus	virus	NN
cord-288669-46tkedw7	336	4	was	be	VBD
cord-288669-46tkedw7	336	5	aliquoted	aliquote	VBN
cord-288669-46tkedw7	336	6	and	and	CC
cord-288669-46tkedw7	336	7	stored	store	VBN
cord-288669-46tkedw7	336	8	at	at	IN
cord-288669-46tkedw7	336	9	−	−	CD
cord-288669-46tkedw7	336	10	80	80	CD
cord-288669-46tkedw7	336	11	°	°	NN
cord-288669-46tkedw7	336	12	C	c	NN
cord-288669-46tkedw7	336	13	until	until	IN
cord-288669-46tkedw7	336	14	use	use	NN
cord-288669-46tkedw7	336	15	.	.	.
cord-288669-46tkedw7	337	1	Culture	culture	NN
cord-288669-46tkedw7	337	2	supernatants	supernatant	NNS
cord-288669-46tkedw7	337	3	of	of	IN
cord-288669-46tkedw7	337	4	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	337	5	cells	cell	NNS
cord-288669-46tkedw7	337	6	transfected	transfecte	VBN
cord-288669-46tkedw7	337	7	with	with	IN
cord-288669-46tkedw7	337	8	the	the	DT
cord-288669-46tkedw7	337	9	full	full	JJ
cord-288669-46tkedw7	337	10	-	-	HYPH
cord-288669-46tkedw7	337	11	length	length	NN
cord-288669-46tkedw7	337	12	cDNA	cdna	NN
cord-288669-46tkedw7	337	13	plasmid	plasmid	NN
cord-288669-46tkedw7	337	14	were	be	VBD
cord-288669-46tkedw7	337	15	harvested	harvest	VBN
cord-288669-46tkedw7	337	16	at	at	IN
cord-288669-46tkedw7	337	17	2	2	CD
cord-288669-46tkedw7	337	18	days	day	NNS
cord-288669-46tkedw7	337	19	posttransfection	posttransfection	NN
cord-288669-46tkedw7	337	20	as	as	IN
cord-288669-46tkedw7	337	21	described	describe	VBN
cord-288669-46tkedw7	337	22	above	above	RB
cord-288669-46tkedw7	337	23	.	.	.
cord-288669-46tkedw7	338	1	The	the	DT
cord-288669-46tkedw7	338	2	culture	culture	NN
cord-288669-46tkedw7	338	3	fluids	fluid	NNS
cord-288669-46tkedw7	338	4	were	be	VBD
cord-288669-46tkedw7	338	5	centrifuged	centrifuge	VBN
cord-288669-46tkedw7	338	6	at	at	IN
cord-288669-46tkedw7	338	7	3500	3500	CD
cord-288669-46tkedw7	338	8	rpm	rpm	NN
cord-288669-46tkedw7	338	9	(	(	-LRB-
cord-288669-46tkedw7	338	10	model	model	NN
cord-288669-46tkedw7	338	11	5415	5415	CD
cord-288669-46tkedw7	338	12	;	;	:
cord-288669-46tkedw7	338	13	Eppendorf	Eppendorf	NNP
cord-288669-46tkedw7	338	14	)	)	-RRB-
cord-288669-46tkedw7	338	15	for	for	IN
cord-288669-46tkedw7	338	16	10	10	CD
cord-288669-46tkedw7	338	17	min	min	NN
cord-288669-46tkedw7	338	18	to	to	TO
cord-288669-46tkedw7	338	19	remove	remove	VB
cord-288669-46tkedw7	338	20	cellular	cellular	JJ
cord-288669-46tkedw7	338	21	debris	debris	NN
cord-288669-46tkedw7	338	22	.	.	.
cord-288669-46tkedw7	339	1	The	the	DT
cord-288669-46tkedw7	339	2	cleared	clear	VBN
cord-288669-46tkedw7	339	3	supernatants	supernatant	NNS
cord-288669-46tkedw7	339	4	were	be	VBD
cord-288669-46tkedw7	339	5	purified	purify	VBN
cord-288669-46tkedw7	339	6	as	as	IN
cord-288669-46tkedw7	339	7	described	describe	VBN
cord-288669-46tkedw7	339	8	above	above	RB
cord-288669-46tkedw7	339	9	,	,	,
cord-288669-46tkedw7	339	10	and	and	CC
cord-288669-46tkedw7	339	11	the	the	DT
cord-288669-46tkedw7	339	12	pellets	pellet	NNS
cord-288669-46tkedw7	339	13	were	be	VBD
cord-288669-46tkedw7	339	14	suspended	suspend	VBN
cord-288669-46tkedw7	339	15	in	in	IN
cord-288669-46tkedw7	339	16	50	50	CD
cord-288669-46tkedw7	339	17	μl	μl	NN
cord-288669-46tkedw7	339	18	of	of	IN
cord-288669-46tkedw7	339	19	PBS	PBS	NNP
cord-288669-46tkedw7	339	20	and	and	CC
cord-288669-46tkedw7	339	21	subsequently	subsequently	RB
cord-288669-46tkedw7	339	22	stored	store	VBD
cord-288669-46tkedw7	339	23	at	at	IN
cord-288669-46tkedw7	339	24	4	4	CD
cord-288669-46tkedw7	339	25	°	°	NN
cord-288669-46tkedw7	339	26	C	c	NN
cord-288669-46tkedw7	339	27	until	until	IN
cord-288669-46tkedw7	339	28	use	use	NN
cord-288669-46tkedw7	339	29	.	.	.
cord-288669-46tkedw7	340	1	Twenty	twenty	CD
cord-288669-46tkedw7	340	2	microliters	microliter	NNS
cord-288669-46tkedw7	340	3	of	of	IN
cord-288669-46tkedw7	340	4	the	the	DT
cord-288669-46tkedw7	340	5	virion	virion	NN
cord-288669-46tkedw7	340	6	suspension	suspension	NN
cord-288669-46tkedw7	340	7	was	be	VBD
cord-288669-46tkedw7	340	8	mounted	mount	VBN
cord-288669-46tkedw7	340	9	on	on	IN
cord-288669-46tkedw7	340	10	a	a	DT
cord-288669-46tkedw7	340	11	Formarcoated	Formarcoated	NNP
cord-288669-46tkedw7	340	12	copper	copper	NN
cord-288669-46tkedw7	340	13	grid	grid	NN
cord-288669-46tkedw7	340	14	.	.	.
cord-288669-46tkedw7	341	1	The	the	DT
cord-288669-46tkedw7	341	2	grids	grid	NNS
cord-288669-46tkedw7	341	3	were	be	VBD
cord-288669-46tkedw7	341	4	placed	place	VBN
cord-288669-46tkedw7	341	5	at	at	IN
cord-288669-46tkedw7	341	6	RT	RT	NNP
cord-288669-46tkedw7	341	7	for	for	IN
cord-288669-46tkedw7	341	8	5	5	CD
cord-288669-46tkedw7	341	9	min	min	NN
cord-288669-46tkedw7	341	10	,	,	,
cord-288669-46tkedw7	341	11	and	and	CC
cord-288669-46tkedw7	341	12	excess	excess	JJ
cord-288669-46tkedw7	341	13	liquid	liquid	NN
cord-288669-46tkedw7	341	14	was	be	VBD
cord-288669-46tkedw7	341	15	removed	remove	VBN
cord-288669-46tkedw7	341	16	by	by	IN
cord-288669-46tkedw7	341	17	wicking	wicke	VBG
cord-288669-46tkedw7	341	18	with	with	IN
cord-288669-46tkedw7	341	19	filter	filter	NN
cord-288669-46tkedw7	341	20	paper	paper	NN
cord-288669-46tkedw7	341	21	.	.	.
cord-288669-46tkedw7	342	1	For	for	IN
cord-288669-46tkedw7	342	2	negative	negative	JJ
cord-288669-46tkedw7	342	3	staining	staining	NN
cord-288669-46tkedw7	342	4	,	,	,
cord-288669-46tkedw7	342	5	20	20	CD
cord-288669-46tkedw7	342	6	μl	μl	NN
cord-288669-46tkedw7	342	7	of	of	IN
cord-288669-46tkedw7	342	8	2	2	CD
cord-288669-46tkedw7	342	9	%	%	NN
cord-288669-46tkedw7	342	10	sodium	sodium	NN
cord-288669-46tkedw7	342	11	phosphotungstate	phosphotungstate	JJ
cord-288669-46tkedw7	342	12	(	(	-LRB-
cord-288669-46tkedw7	342	13	pH	pH	NNP
cord-288669-46tkedw7	342	14	6.8	6.8	CD
cord-288669-46tkedw7	342	15	)	)	-RRB-
cord-288669-46tkedw7	342	16	was	be	VBD
cord-288669-46tkedw7	342	17	dropped	drop	VBN
cord-288669-46tkedw7	342	18	onto	onto	IN
cord-288669-46tkedw7	342	19	the	the	DT
cord-288669-46tkedw7	342	20	grids	grid	NNS
cord-288669-46tkedw7	342	21	and	and	CC
cord-288669-46tkedw7	342	22	incubated	incubate	VBN
cord-288669-46tkedw7	342	23	at	at	IN
cord-288669-46tkedw7	342	24	RT	RT	NNP
cord-288669-46tkedw7	342	25	for	for	IN
cord-288669-46tkedw7	342	26	30	30	CD
cord-288669-46tkedw7	342	27	s.	s.	NN
cord-288669-46tkedw7	343	1	The	the	DT
cord-288669-46tkedw7	343	2	samples	sample	NNS
cord-288669-46tkedw7	343	3	were	be	VBD
cord-288669-46tkedw7	343	4	viewed	view	VBN
cord-288669-46tkedw7	343	5	with	with	IN
cord-288669-46tkedw7	343	6	a	a	DT
cord-288669-46tkedw7	343	7	transmission	transmission	NN
cord-288669-46tkedw7	343	8	electron	electron	NN
cord-288669-46tkedw7	343	9	microscope	microscope	NN
cord-288669-46tkedw7	343	10	(	(	-LRB-
cord-288669-46tkedw7	343	11	model	model	NN
cord-288669-46tkedw7	343	12	H-7600	h-7600	DT
cord-288669-46tkedw7	343	13	120	120	CD
cord-288669-46tkedw7	343	14	keV	keV	NNP
cord-288669-46tkedw7	343	15	PC	pc	NN
cord-288669-46tkedw7	343	16	-	-	HYPH
cord-288669-46tkedw7	343	17	TEM	tem	NN
cord-288669-46tkedw7	343	18	;	;	:
cord-288669-46tkedw7	343	19	Hitachi	Hitachi	NNP
cord-288669-46tkedw7	343	20	)	)	-RRB-
cord-288669-46tkedw7	343	21	operating	operate	VBG
cord-288669-46tkedw7	343	22	at	at	IN
cord-288669-46tkedw7	343	23	75	75	CD
cord-288669-46tkedw7	343	24	keV.	keV.	NNS
cord-288669-46tkedw7	343	25	Viral	Viral	NNP
cord-288669-46tkedw7	343	26	RNA	RNA	NNP
cord-288669-46tkedw7	343	27	was	be	VBD
cord-288669-46tkedw7	343	28	extracted	extract	VBN
cord-288669-46tkedw7	343	29	from	from	IN
cord-288669-46tkedw7	343	30	either	either	DT
cord-288669-46tkedw7	343	31	supernatants	supernatant	NNS
cord-288669-46tkedw7	343	32	or	or	CC
cord-288669-46tkedw7	343	33	lysates	lysate	NNS
cord-288669-46tkedw7	343	34	of	of	IN
cord-288669-46tkedw7	343	35	infected	infected	JJ
cord-288669-46tkedw7	343	36	cells	cell	NNS
cord-288669-46tkedw7	343	37	using	use	VBG
cord-288669-46tkedw7	343	38	the	the	DT
cord-288669-46tkedw7	343	39	QiaAmp	QiaAmp	NNP
cord-288669-46tkedw7	343	40	viral	viral	JJ
cord-288669-46tkedw7	343	41	RNA	rna	NN
cord-288669-46tkedw7	343	42	mini	mini	NN
cord-288669-46tkedw7	343	43	-	-	NN
cord-288669-46tkedw7	343	44	kit	kit	NN
cord-288669-46tkedw7	343	45	(	(	-LRB-
cord-288669-46tkedw7	343	46	Qiagen	Qiagen	NNP
cord-288669-46tkedw7	343	47	)	)	-RRB-
cord-288669-46tkedw7	343	48	.	.	.
cord-288669-46tkedw7	344	1	To	to	TO
cord-288669-46tkedw7	344	2	remove	remove	VB
cord-288669-46tkedw7	344	3	any	any	DT
cord-288669-46tkedw7	344	4	contaminated	contaminate	VBN
cord-288669-46tkedw7	344	5	DNA	dna	NN
cord-288669-46tkedw7	344	6	in	in	IN
cord-288669-46tkedw7	344	7	the	the	DT
cord-288669-46tkedw7	344	8	RNA	RNA	NNP
cord-288669-46tkedw7	344	9	preparations	preparation	NNS
cord-288669-46tkedw7	344	10	,	,	,
cord-288669-46tkedw7	344	11	samples	sample	NNS
cord-288669-46tkedw7	344	12	were	be	VBD
cord-288669-46tkedw7	344	13	treated	treat	VBN
cord-288669-46tkedw7	344	14	with	with	IN
cord-288669-46tkedw7	344	15	1	1	CD
cord-288669-46tkedw7	344	16	U	u	NN
cord-288669-46tkedw7	344	17	of	of	IN
cord-288669-46tkedw7	344	18	RQ	rq	NN
cord-288669-46tkedw7	345	1	DNase	DNase	NNP
cord-288669-46tkedw7	345	2	I	i	NN
cord-288669-46tkedw7	345	3	(	(	-LRB-
cord-288669-46tkedw7	345	4	Promega	Promega	NNP
cord-288669-46tkedw7	345	5	)	)	-RRB-
cord-288669-46tkedw7	345	6	at	at	IN
cord-288669-46tkedw7	345	7	37	37	CD
cord-288669-46tkedw7	345	8	°	°	NN
cord-288669-46tkedw7	345	9	C	c	NN
cord-288669-46tkedw7	345	10	for	for	IN
cord-288669-46tkedw7	345	11	30	30	CD
cord-288669-46tkedw7	345	12	min	min	NN
cord-288669-46tkedw7	345	13	in	in	IN
cord-288669-46tkedw7	345	14	50	50	CD
cord-288669-46tkedw7	345	15	mM	mm	CD
cord-288669-46tkedw7	345	16	Tris	Tris	NNP
cord-288669-46tkedw7	345	17	-	-	HYPH
cord-288669-46tkedw7	345	18	HCl	HCl	NNP
cord-288669-46tkedw7	345	19	[	[	-LRB-
cord-288669-46tkedw7	345	20	pH	pH	NNP
cord-288669-46tkedw7	345	21	7.5	7.5	CD
cord-288669-46tkedw7	345	22	]	]	-RRB-
cord-288669-46tkedw7	345	23	and	and	CC
cord-288669-46tkedw7	345	24	1	1	CD
cord-288669-46tkedw7	345	25	mM	mm	CD
cord-288669-46tkedw7	345	26	MgCl	MgCl	NNP
cord-288669-46tkedw7	345	27	2	2	CD
cord-288669-46tkedw7	345	28	.	.	.
cord-288669-46tkedw7	346	1	For	for	IN
cord-288669-46tkedw7	346	2	detection	detection	NN
cord-288669-46tkedw7	346	3	of	of	IN
cord-288669-46tkedw7	346	4	viral	viral	JJ
cord-288669-46tkedw7	346	5	RNA	RNA	NNP
cord-288669-46tkedw7	346	6	in	in	IN
cord-288669-46tkedw7	346	7	the	the	DT
cord-288669-46tkedw7	346	8	virions	virion	NNS
cord-288669-46tkedw7	346	9	,	,	,
cord-288669-46tkedw7	346	10	samples	sample	NNS
cord-288669-46tkedw7	346	11	were	be	VBD
cord-288669-46tkedw7	346	12	prepared	prepare	VBN
cord-288669-46tkedw7	346	13	as	as	IN
cord-288669-46tkedw7	346	14	previously	previously	RB
cord-288669-46tkedw7	346	15	described	describe	VBN
cord-288669-46tkedw7	347	1	(	(	-LRB-
cord-288669-46tkedw7	347	2	Wieringa	Wieringa	NNP
cord-288669-46tkedw7	347	3	et	et	NNP
cord-288669-46tkedw7	347	4	al	al	NNP
cord-288669-46tkedw7	347	5	.	.	NNP
cord-288669-46tkedw7	347	6	,	,	,
cord-288669-46tkedw7	347	7	2004	2004	CD
cord-288669-46tkedw7	347	8	)	)	-RRB-
cord-288669-46tkedw7	347	9	with	with	IN
cord-288669-46tkedw7	347	10	some	some	DT
cord-288669-46tkedw7	347	11	modifications	modification	NNS
cord-288669-46tkedw7	347	12	.	.	.
cord-288669-46tkedw7	348	1	The	the	DT
cord-288669-46tkedw7	348	2	viral	viral	JJ
cord-288669-46tkedw7	348	3	particles	particle	NNS
cord-288669-46tkedw7	348	4	in	in	IN
cord-288669-46tkedw7	348	5	the	the	DT
cord-288669-46tkedw7	348	6	culture	culture	NN
cord-288669-46tkedw7	348	7	supernatant	supernatant	VBN
cord-288669-46tkedw7	348	8	from	from	IN
cord-288669-46tkedw7	348	9	BHK-21	BHK-21	NNP
cord-288669-46tkedw7	348	10	cells	cell	NNS
cord-288669-46tkedw7	348	11	transfected	transfecte	VBN
cord-288669-46tkedw7	348	12	with	with	IN
cord-288669-46tkedw7	348	13	the	the	DT
cord-288669-46tkedw7	348	14	full	full	JJ
cord-288669-46tkedw7	348	15	-	-	HYPH
cord-288669-46tkedw7	348	16	length	length	NN
cord-288669-46tkedw7	348	17	cDNA	cdna	NN
cord-288669-46tkedw7	348	18	plasmid	plasmid	NN
cord-288669-46tkedw7	348	19	were	be	VBD
cord-288669-46tkedw7	348	20	pelleted	pellete	VBN
cord-288669-46tkedw7	348	21	by	by	IN
cord-288669-46tkedw7	348	22	as	as	IN
cord-288669-46tkedw7	348	23	described	describe	VBN
cord-288669-46tkedw7	348	24	above	above	RB
cord-288669-46tkedw7	348	25	.	.	.
cord-288669-46tkedw7	349	1	The	the	DT
cord-288669-46tkedw7	349	2	pellet	pellet	NN
cord-288669-46tkedw7	349	3	was	be	VBD
cord-288669-46tkedw7	349	4	resuspended	resuspend	VBN
cord-288669-46tkedw7	349	5	in	in	IN
cord-288669-46tkedw7	349	6	TNM	TNM	NNP
cord-288669-46tkedw7	349	7	buffer	buffer	NN
cord-288669-46tkedw7	349	8	(	(	-LRB-
cord-288669-46tkedw7	349	9	20	20	CD
cord-288669-46tkedw7	349	10	mM	mM	NNP
cord-288669-46tkedw7	349	11	Tris	Tris	NNP
cord-288669-46tkedw7	349	12	-	-	HYPH
cord-288669-46tkedw7	349	13	HCl	HCl	NNP
cord-288669-46tkedw7	349	14	[	[	-LRB-
cord-288669-46tkedw7	349	15	pH	pH	NNP
cord-288669-46tkedw7	349	16	7.5	7.5	CD
cord-288669-46tkedw7	349	17	]	]	-RRB-
cord-288669-46tkedw7	349	18	,	,	,
cord-288669-46tkedw7	349	19	50	50	CD
cord-288669-46tkedw7	349	20	mM	mm	CD
cord-288669-46tkedw7	349	21	NaCl	nacl	NN
cord-288669-46tkedw7	349	22	,	,	,
cord-288669-46tkedw7	349	23	5	5	CD
cord-288669-46tkedw7	349	24	mM	mm	CD
cord-288669-46tkedw7	349	25	MgCl	MgCl	NNP
cord-288669-46tkedw7	349	26	2	2	CD
cord-288669-46tkedw7	349	27	)	)	-RRB-
cord-288669-46tkedw7	349	28	.	.	.
cord-288669-46tkedw7	350	1	Seventy	seventy	CD
cord-288669-46tkedw7	350	2	microliters	microliter	NNS
cord-288669-46tkedw7	350	3	of	of	IN
cord-288669-46tkedw7	350	4	each	each	DT
cord-288669-46tkedw7	350	5	sample	sample	NN
cord-288669-46tkedw7	350	6	suspension	suspension	NN
cord-288669-46tkedw7	350	7	were	be	VBD
cord-288669-46tkedw7	350	8	added	add	VBN
cord-288669-46tkedw7	350	9	with	with	IN
cord-288669-46tkedw7	350	10	2	2	CD
cord-288669-46tkedw7	350	11	μl	μl	NN
cord-288669-46tkedw7	350	12	of	of	IN
cord-288669-46tkedw7	350	13	RQ	rq	NN
cord-288669-46tkedw7	351	1	DNase	DNase	NNP
cord-288669-46tkedw7	351	2	I	i	NN
cord-288669-46tkedw7	351	3	(	(	-LRB-
cord-288669-46tkedw7	351	4	1	1	CD
cord-288669-46tkedw7	351	5	U	u	NN
cord-288669-46tkedw7	351	6	/	/	SYM
cord-288669-46tkedw7	351	7	μl	μl	XX
cord-288669-46tkedw7	351	8	;	;	:
cord-288669-46tkedw7	351	9	Promega	Promega	NNP
cord-288669-46tkedw7	351	10	)	)	-RRB-
cord-288669-46tkedw7	351	11	and	and	CC
cord-288669-46tkedw7	351	12	3.6	3.6	CD
cord-288669-46tkedw7	351	13	μl	μl	NN
cord-288669-46tkedw7	351	14	of	of	IN
cord-288669-46tkedw7	351	15	RNase	RNase	NNP
cord-288669-46tkedw7	351	16	A	A	NNP
cord-288669-46tkedw7	351	17	(	(	-LRB-
cord-288669-46tkedw7	351	18	10	10	CD
cord-288669-46tkedw7	351	19	mg	mg	NNP
cord-288669-46tkedw7	351	20	/	/	SYM
cord-288669-46tkedw7	351	21	ml	ml	NN
cord-288669-46tkedw7	351	22	;	;	:
cord-288669-46tkedw7	351	23	Sigma	Sigma	NNP
cord-288669-46tkedw7	351	24	)	)	-RRB-
cord-288669-46tkedw7	351	25	,	,	,
cord-288669-46tkedw7	351	26	and	and	CC
cord-288669-46tkedw7	351	27	the	the	DT
cord-288669-46tkedw7	351	28	mixture	mixture	NN
cord-288669-46tkedw7	351	29	was	be	VBD
cord-288669-46tkedw7	351	30	incubated	incubate	VBN
cord-288669-46tkedw7	351	31	for	for	IN
cord-288669-46tkedw7	351	32	1	1	CD
cord-288669-46tkedw7	351	33	h	h	NN
cord-288669-46tkedw7	351	34	at	at	IN
cord-288669-46tkedw7	351	35	37	37	CD
cord-288669-46tkedw7	351	36	°	°	NN
cord-288669-46tkedw7	351	37	C	c	NN
cord-288669-46tkedw7	351	38	in	in	IN
cord-288669-46tkedw7	351	39	the	the	DT
cord-288669-46tkedw7	351	40	presence	presence	NN
cord-288669-46tkedw7	351	41	or	or	CC
cord-288669-46tkedw7	351	42	absence	absence	NN
cord-288669-46tkedw7	351	43	of	of	IN
cord-288669-46tkedw7	351	44	detergents	detergent	NNS
cord-288669-46tkedw7	351	45	(	(	-LRB-
cord-288669-46tkedw7	351	46	1	1	CD
cord-288669-46tkedw7	351	47	μl	μl	NN
cord-288669-46tkedw7	351	48	of	of	IN
cord-288669-46tkedw7	351	49	Triton	Triton	NNP
cord-288669-46tkedw7	351	50	X-100	x-100	NN
cord-288669-46tkedw7	351	51	,	,	,
cord-288669-46tkedw7	351	52	and	and	CC
cord-288669-46tkedw7	351	53	3.5	3.5	CD
cord-288669-46tkedw7	351	54	μl	μl	NN
cord-288669-46tkedw7	351	55	of	of	IN
cord-288669-46tkedw7	351	56	10	10	CD
cord-288669-46tkedw7	351	57	%	%	NN
cord-288669-46tkedw7	351	58	SDS	SDS	NNP
cord-288669-46tkedw7	351	59	)	)	-RRB-
cord-288669-46tkedw7	351	60	.	.	.
cord-288669-46tkedw7	352	1	After	after	IN
cord-288669-46tkedw7	352	2	incubation	incubation	NN
cord-288669-46tkedw7	352	3	for	for	IN
cord-288669-46tkedw7	352	4	1	1	CD
cord-288669-46tkedw7	352	5	h	h	NN
cord-288669-46tkedw7	352	6	at	at	IN
cord-288669-46tkedw7	352	7	37	37	CD
cord-288669-46tkedw7	352	8	°	°	NN
cord-288669-46tkedw7	352	9	C	c	NN
cord-288669-46tkedw7	352	10	,	,	,
cord-288669-46tkedw7	352	11	the	the	DT
cord-288669-46tkedw7	352	12	nucleases	nuclease	NNS
cord-288669-46tkedw7	352	13	were	be	VBD
cord-288669-46tkedw7	352	14	inactivated	inactivate	VBN
cord-288669-46tkedw7	352	15	by	by	IN
cord-288669-46tkedw7	352	16	the	the	DT
cord-288669-46tkedw7	352	17	addition	addition	NN
cord-288669-46tkedw7	352	18	of	of	IN
cord-288669-46tkedw7	352	19	5	5	CD
cord-288669-46tkedw7	352	20	μl	μl	NN
cord-288669-46tkedw7	352	21	of	of	IN
cord-288669-46tkedw7	352	22	proteinase	proteinase	NN
cord-288669-46tkedw7	352	23	K	K	NNP
cord-288669-46tkedw7	352	24	(	(	-LRB-
cord-288669-46tkedw7	352	25	20	20	CD
cord-288669-46tkedw7	352	26	mg	mg	NNP
cord-288669-46tkedw7	352	27	/	/	SYM
cord-288669-46tkedw7	352	28	ml	ml	NN
cord-288669-46tkedw7	352	29	;	;	:
cord-288669-46tkedw7	352	30	Qiagen	Qiagen	NNP
cord-288669-46tkedw7	352	31	)	)	-RRB-
cord-288669-46tkedw7	352	32	and	and	CC
cord-288669-46tkedw7	352	33	incubation	incubation	NN
cord-288669-46tkedw7	352	34	for	for	IN
cord-288669-46tkedw7	352	35	30	30	CD
cord-288669-46tkedw7	352	36	min	min	NN
cord-288669-46tkedw7	352	37	at	at	IN
cord-288669-46tkedw7	352	38	50	50	CD
cord-288669-46tkedw7	352	39	°	°	NN
cord-288669-46tkedw7	352	40	C	c	NN
cord-288669-46tkedw7	352	41	.	.	.
cord-288669-46tkedw7	353	1	RNA	RNA	NNP
cord-288669-46tkedw7	353	2	was	be	VBD
cord-288669-46tkedw7	353	3	isolated	isolate	VBN
cord-288669-46tkedw7	353	4	from	from	IN
cord-288669-46tkedw7	353	5	each	each	DT
cord-288669-46tkedw7	353	6	sample	sample	NN
cord-288669-46tkedw7	353	7	by	by	IN
cord-288669-46tkedw7	353	8	using	use	VBG
cord-288669-46tkedw7	353	9	a	a	DT
cord-288669-46tkedw7	353	10	QIAamp	QIAamp	NNP
cord-288669-46tkedw7	353	11	viral	viral	JJ
cord-288669-46tkedw7	353	12	RNA	rna	NN
cord-288669-46tkedw7	353	13	mini	mini	NN
cord-288669-46tkedw7	353	14	-	-	NN
cord-288669-46tkedw7	353	15	kit	kit	NN
cord-288669-46tkedw7	353	16	and	and	CC
cord-288669-46tkedw7	353	17	subsequently	subsequently	RB
cord-288669-46tkedw7	353	18	,	,	,
cord-288669-46tkedw7	353	19	treated	treat	VBD
cord-288669-46tkedw7	353	20	one	one	CD
cord-288669-46tkedw7	353	21	more	more	JJR
cord-288669-46tkedw7	353	22	time	time	NN
cord-288669-46tkedw7	353	23	with	with	IN
cord-288669-46tkedw7	353	24	1	1	CD
cord-288669-46tkedw7	353	25	unit	unit	NN
cord-288669-46tkedw7	353	26	of	of	IN
cord-288669-46tkedw7	353	27	RQ	RQ	NNP
cord-288669-46tkedw7	353	28	DNase	DNase	NNP
cord-288669-46tkedw7	353	29	I.	i.	NN
cord-288669-46tkedw7	354	1	After	after	IN
cord-288669-46tkedw7	354	2	DNase	DNase	NNP
cord-288669-46tkedw7	355	1	I	-PRON-	PRP
cord-288669-46tkedw7	355	2	treatment	treatment	NN
cord-288669-46tkedw7	355	3	,	,	,
cord-288669-46tkedw7	355	4	RNA	RNA	NNP
cord-288669-46tkedw7	355	5	was	be	VBD
cord-288669-46tkedw7	355	6	re	re	VBN
cord-288669-46tkedw7	355	7	-	-	VBN
cord-288669-46tkedw7	355	8	extracted	extract	VBN
cord-288669-46tkedw7	355	9	with	with	IN
cord-288669-46tkedw7	355	10	an	an	DT
cord-288669-46tkedw7	355	11	equal	equal	JJ
cord-288669-46tkedw7	355	12	volume	volume	NN
cord-288669-46tkedw7	355	13	of	of	IN
cord-288669-46tkedw7	355	14	phenol	phenol	NN
cord-288669-46tkedw7	355	15	:	:	:
cord-288669-46tkedw7	355	16	chloroform	chloroform	NN
cord-288669-46tkedw7	355	17	:	:	:
cord-288669-46tkedw7	355	18	isoamyl	isoamyl	NN
cord-288669-46tkedw7	355	19	alcohol	alcohol	NN
cord-288669-46tkedw7	355	20	(	(	-LRB-
cord-288669-46tkedw7	355	21	25:24:1	25:24:1	LS
cord-288669-46tkedw7	355	22	)	)	-RRB-
cord-288669-46tkedw7	355	23	mixture	mixture	NN
cord-288669-46tkedw7	355	24	and	and	CC
cord-288669-46tkedw7	355	25	precipitated	precipitate	VBN
cord-288669-46tkedw7	355	26	at	at	IN
cord-288669-46tkedw7	355	27	−	−	CD
cord-288669-46tkedw7	355	28	80	80	CD
cord-288669-46tkedw7	355	29	°	°	NN
cord-288669-46tkedw7	355	30	C	c	NN
cord-288669-46tkedw7	355	31	for	for	IN
cord-288669-46tkedw7	355	32	1	1	CD
cord-288669-46tkedw7	355	33	h	h	NN
cord-288669-46tkedw7	355	34	by	by	IN
cord-288669-46tkedw7	355	35	adding	add	VBG
cord-288669-46tkedw7	355	36	0.1	0.1	CD
cord-288669-46tkedw7	355	37	volume	volume	NN
cord-288669-46tkedw7	355	38	of	of	IN
cord-288669-46tkedw7	355	39	2.5	2.5	CD
cord-288669-46tkedw7	355	40	M	M	NNP
cord-288669-46tkedw7	355	41	sodium	sodium	NN
cord-288669-46tkedw7	355	42	acetate	acetate	NN
cord-288669-46tkedw7	355	43	(	(	-LRB-
cord-288669-46tkedw7	355	44	pH	pH	NNP
cord-288669-46tkedw7	355	45	5.2	5.2	CD
cord-288669-46tkedw7	355	46	)	)	-RRB-
cord-288669-46tkedw7	355	47	and	and	CC
cord-288669-46tkedw7	355	48	2	2	CD
cord-288669-46tkedw7	355	49	volumes	volume	NNS
cord-288669-46tkedw7	355	50	of	of	IN
cord-288669-46tkedw7	355	51	ethanol	ethanol	NN
cord-288669-46tkedw7	355	52	.	.	.
cord-288669-46tkedw7	356	1	The	the	DT
cord-288669-46tkedw7	356	2	pellets	pellet	NNS
cord-288669-46tkedw7	356	3	were	be	VBD
cord-288669-46tkedw7	356	4	washed	wash	VBN
cord-288669-46tkedw7	356	5	once	once	RB
cord-288669-46tkedw7	356	6	with	with	IN
cord-288669-46tkedw7	356	7	70	70	CD
cord-288669-46tkedw7	356	8	%	%	NN
cord-288669-46tkedw7	356	9	ethanol	ethanol	NN
cord-288669-46tkedw7	356	10	and	and	CC
cord-288669-46tkedw7	356	11	dissolved	dissolve	VBN
cord-288669-46tkedw7	356	12	in	in	IN
cord-288669-46tkedw7	356	13	UltraPure	UltraPure	NNP
cord-288669-46tkedw7	356	14	DNase	DNase	NNP
cord-288669-46tkedw7	356	15	/	/	SYM
cord-288669-46tkedw7	356	16	RNase	RNase	NNP
cord-288669-46tkedw7	356	17	-	-	HYPH
cord-288669-46tkedw7	356	18	Free	Free	NNP
cord-288669-46tkedw7	356	19	Distilled	Distilled	NNP
cord-288669-46tkedw7	356	20	Water	Water	NNP
cord-288669-46tkedw7	356	21	(	(	-LRB-
cord-288669-46tkedw7	356	22	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	356	23	)	)	-RRB-
cord-288669-46tkedw7	356	24	.	.	.
cord-288669-46tkedw7	357	1	The	the	DT
cord-288669-46tkedw7	357	2	resulting	result	VBG
cord-288669-46tkedw7	357	3	RNA	RNA	NNP
cord-288669-46tkedw7	357	4	samples	sample	NNS
cord-288669-46tkedw7	357	5	were	be	VBD
cord-288669-46tkedw7	357	6	used	use	VBN
cord-288669-46tkedw7	357	7	for	for	IN
cord-288669-46tkedw7	357	8	first	first	JJ
cord-288669-46tkedw7	357	9	-	-	HYPH
cord-288669-46tkedw7	357	10	strand	strand	NN
cord-288669-46tkedw7	357	11	cDNA	cdna	NN
cord-288669-46tkedw7	357	12	synthesis	synthesis	NN
cord-288669-46tkedw7	357	13	by	by	IN
cord-288669-46tkedw7	357	14	Moloney	Moloney	NNP
cord-288669-46tkedw7	357	15	Murine	Murine	NNP
cord-288669-46tkedw7	357	16	Leukemia	Leukemia	NNP
cord-288669-46tkedw7	357	17	Virus	Virus	NNP
cord-288669-46tkedw7	357	18	(	(	-LRB-
cord-288669-46tkedw7	357	19	M	M	NNP
cord-288669-46tkedw7	357	20	-	-	HYPH
cord-288669-46tkedw7	357	21	MLV	MLV	NNP
cord-288669-46tkedw7	357	22	)	)	-RRB-
cord-288669-46tkedw7	358	1	reverse	reverse	JJ
cord-288669-46tkedw7	358	2	transcriptase	transcriptase	NN
cord-288669-46tkedw7	358	3	(	(	-LRB-
cord-288669-46tkedw7	358	4	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	358	5	)	)	-RRB-
cord-288669-46tkedw7	358	6	using	use	VBG
cord-288669-46tkedw7	358	7	the	the	DT
cord-288669-46tkedw7	358	8	reverse	reverse	JJ
cord-288669-46tkedw7	358	9	primer	primer	NN
cord-288669-46tkedw7	358	10	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	358	11	-	-	HYPH
cord-288669-46tkedw7	358	12	Rev	Rev	NNP
cord-288669-46tkedw7	358	13	(	(	-LRB-
cord-288669-46tkedw7	358	14	5′-TCATAAGATCTTCTGTAATTGCTC-3′	5′-tcataagatcttctgtaattgctc-3′	CD
cord-288669-46tkedw7	358	15	)	)	-RRB-
cord-288669-46tkedw7	358	16	.	.	.
cord-288669-46tkedw7	359	1	The	the	DT
cord-288669-46tkedw7	359	2	E	e	NN
cord-288669-46tkedw7	359	3	gene	gene	NN
cord-288669-46tkedw7	359	4	was	be	VBD
cord-288669-46tkedw7	359	5	amplified	amplify	VBN
cord-288669-46tkedw7	359	6	by	by	IN
cord-288669-46tkedw7	359	7	Taq	Taq	NNP
cord-288669-46tkedw7	359	8	DNA	dna	NN
cord-288669-46tkedw7	359	9	polymerase	polymerase	NN
cord-288669-46tkedw7	359	10	(	(	-LRB-
cord-288669-46tkedw7	359	11	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	359	12	)	)	-RRB-
cord-288669-46tkedw7	359	13	using	use	VBG
cord-288669-46tkedw7	359	14	mRNA2-Fwd	mrna2-fwd	VB
cord-288669-46tkedw7	359	15	(	(	-LRB-
cord-288669-46tkedw7	359	16	5′-CCGTCATTGAAC	5′-ccgtcattgaac	CD
cord-288669-46tkedw7	359	17	-	-	HYPH
cord-288669-46tkedw7	359	18	CAACTTTA-3′	CAACTTTA-3′	NNP
cord-288669-46tkedw7	359	19	)	)	-RRB-
cord-288669-46tkedw7	359	20	and	and	CC
cord-288669-46tkedw7	359	21	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	359	22	-	-	HYPH
cord-288669-46tkedw7	359	23	Rev	Rev	NNP
cord-288669-46tkedw7	359	24	.	.	.
cord-288669-46tkedw7	360	1	For	for	IN
cord-288669-46tkedw7	360	2	strand	strand	NN
cord-288669-46tkedw7	360	3	-	-	HYPH
cord-288669-46tkedw7	360	4	specific	specific	JJ
cord-288669-46tkedw7	360	5	RT	RT	NNP
cord-288669-46tkedw7	360	6	-	-	HYPH
cord-288669-46tkedw7	360	7	PCR	PCR	NNP
cord-288669-46tkedw7	360	8	,	,	,
cord-288669-46tkedw7	360	9	the	the	DT
cord-288669-46tkedw7	360	10	specific	specific	JJ
cord-288669-46tkedw7	360	11	primer	primer	NN
cord-288669-46tkedw7	360	12	pairs	pair	NNS
cord-288669-46tkedw7	360	13	(	(	-LRB-
cord-288669-46tkedw7	360	14	Table	table	NN
cord-288669-46tkedw7	360	15	1	1	CD
cord-288669-46tkedw7	360	16	)	)	-RRB-
cord-288669-46tkedw7	360	17	were	be	VBD
cord-288669-46tkedw7	360	18	used	use	VBN
cord-288669-46tkedw7	360	19	to	to	TO
cord-288669-46tkedw7	360	20	amplify	amplify	VB
cord-288669-46tkedw7	360	21	DNA	dna	NN
cord-288669-46tkedw7	360	22	fragments	fragment	NNS
cord-288669-46tkedw7	360	23	representing	represent	VBG
cord-288669-46tkedw7	360	24	positive	positive	JJ
cord-288669-46tkedw7	360	25	-	-	HYPH
cord-288669-46tkedw7	360	26	sense	sense	NN
cord-288669-46tkedw7	360	27	genomic	genomic	JJ
cord-288669-46tkedw7	360	28	RNA	RNA	NNP
cord-288669-46tkedw7	360	29	,	,	,
cord-288669-46tkedw7	360	30	negative	negative	JJ
cord-288669-46tkedw7	360	31	-	-	HYPH
cord-288669-46tkedw7	360	32	sense	sense	NN
cord-288669-46tkedw7	360	33	genomic	genomic	JJ
cord-288669-46tkedw7	360	34	RNA	RNA	NNP
cord-288669-46tkedw7	360	35	,	,	,
cord-288669-46tkedw7	360	36	positive	positive	JJ
cord-288669-46tkedw7	360	37	-	-	HYPH
cord-288669-46tkedw7	360	38	sense	sense	NN
cord-288669-46tkedw7	360	39	subgenomic	subgenomic	JJ
cord-288669-46tkedw7	360	40	mRNA7	mrna7	UH
cord-288669-46tkedw7	360	41	or	or	CC
cord-288669-46tkedw7	360	42	negative	negative	JJ
cord-288669-46tkedw7	360	43	-	-	HYPH
cord-288669-46tkedw7	360	44	sense	sense	NN
cord-288669-46tkedw7	360	45	subgenomic	subgenomic	JJ
cord-288669-46tkedw7	360	46	mRNA7	mrna7	ADD
cord-288669-46tkedw7	360	47	.	.	.
cord-288669-46tkedw7	361	1	PCR	PCR	NNP
cord-288669-46tkedw7	361	2	was	be	VBD
cord-288669-46tkedw7	361	3	conducted	conduct	VBN
cord-288669-46tkedw7	361	4	under	under	IN
cord-288669-46tkedw7	361	5	the	the	DT
cord-288669-46tkedw7	361	6	following	follow	VBG
cord-288669-46tkedw7	361	7	conditions	condition	NNS
cord-288669-46tkedw7	361	8	;	;	,
cord-288669-46tkedw7	361	9	initial	initial	JJ
cord-288669-46tkedw7	361	10	denaturation	denaturation	NN
cord-288669-46tkedw7	361	11	at	at	IN
cord-288669-46tkedw7	361	12	95	95	CD
cord-288669-46tkedw7	361	13	°	°	NN
cord-288669-46tkedw7	361	14	C	c	NN
cord-288669-46tkedw7	361	15	for	for	IN
cord-288669-46tkedw7	361	16	5	5	CD
cord-288669-46tkedw7	361	17	min	min	NN
cord-288669-46tkedw7	361	18	,	,	,
cord-288669-46tkedw7	361	19	35	35	CD
cord-288669-46tkedw7	361	20	cycles	cycle	NNS
cord-288669-46tkedw7	361	21	of	of	IN
cord-288669-46tkedw7	361	22	denaturation	denaturation	NN
cord-288669-46tkedw7	361	23	at	at	IN
cord-288669-46tkedw7	361	24	95	95	CD
cord-288669-46tkedw7	361	25	°	°	NN
cord-288669-46tkedw7	361	26	C	c	NN
cord-288669-46tkedw7	361	27	for	for	IN
cord-288669-46tkedw7	361	28	30	30	CD
cord-288669-46tkedw7	361	29	s	s	NN
cord-288669-46tkedw7	361	30	,	,	,
cord-288669-46tkedw7	361	31	annealing	anneal	VBG
cord-288669-46tkedw7	361	32	at	at	IN
cord-288669-46tkedw7	361	33	56	56	CD
cord-288669-46tkedw7	361	34	°	°	NN
cord-288669-46tkedw7	361	35	C	c	NN
cord-288669-46tkedw7	361	36	for	for	IN
cord-288669-46tkedw7	361	37	30	30	CD
cord-288669-46tkedw7	361	38	s	s	NN
cord-288669-46tkedw7	361	39	and	and	CC
cord-288669-46tkedw7	361	40	extension	extension	NN
cord-288669-46tkedw7	361	41	at	at	IN
cord-288669-46tkedw7	361	42	72	72	CD
cord-288669-46tkedw7	361	43	°	°	NN
cord-288669-46tkedw7	361	44	C	c	NN
cord-288669-46tkedw7	361	45	for	for	IN
cord-288669-46tkedw7	361	46	1	1	CD
cord-288669-46tkedw7	361	47	min	min	NN
cord-288669-46tkedw7	361	48	,	,	,
cord-288669-46tkedw7	361	49	followed	follow	VBN
cord-288669-46tkedw7	361	50	by	by	IN
cord-288669-46tkedw7	361	51	a	a	DT
cord-288669-46tkedw7	361	52	final	final	JJ
cord-288669-46tkedw7	361	53	extension	extension	NN
cord-288669-46tkedw7	361	54	at	at	IN
cord-288669-46tkedw7	361	55	72	72	CD
cord-288669-46tkedw7	361	56	°	°	NN
cord-288669-46tkedw7	361	57	C	c	NN
cord-288669-46tkedw7	361	58	for	for	IN
cord-288669-46tkedw7	361	59	10	10	CD
cord-288669-46tkedw7	361	60	min	min	NN
cord-288669-46tkedw7	361	61	.	.	.
cord-288669-46tkedw7	362	1	PCR	PCR	NNP
cord-288669-46tkedw7	362	2	products	product	NNS
cord-288669-46tkedw7	362	3	were	be	VBD
cord-288669-46tkedw7	362	4	analyzed	analyze	VBN
cord-288669-46tkedw7	362	5	by	by	IN
cord-288669-46tkedw7	362	6	0.8	0.8	CD
cord-288669-46tkedw7	362	7	%	%	NN
cord-288669-46tkedw7	362	8	or	or	CC
cord-288669-46tkedw7	362	9	1.5	1.5	CD
cord-288669-46tkedw7	362	10	%	%	NN
cord-288669-46tkedw7	362	11	agarose	agarose	VBD
cord-288669-46tkedw7	362	12	gel	gel	NN
cord-288669-46tkedw7	362	13	electrophoresis	electrophoresis	NN
cord-288669-46tkedw7	362	14	depending	depend	VBG
cord-288669-46tkedw7	362	15	on	on	IN
cord-288669-46tkedw7	362	16	size	size	NN
cord-288669-46tkedw7	362	17	of	of	IN
cord-288669-46tkedw7	362	18	the	the	DT
cord-288669-46tkedw7	362	19	fragment	fragment	NN
cord-288669-46tkedw7	362	20	.	.	.
cord-288669-46tkedw7	363	1	Amplified	amplified	JJ
cord-288669-46tkedw7	363	2	products	product	NNS
cord-288669-46tkedw7	363	3	were	be	VBD
cord-288669-46tkedw7	363	4	purified	purify	VBN
cord-288669-46tkedw7	363	5	using	use	VBG
cord-288669-46tkedw7	363	6	the	the	DT
cord-288669-46tkedw7	363	7	PCR	PCR	NNP
cord-288669-46tkedw7	363	8	purification	purification	NN
cord-288669-46tkedw7	363	9	kit	kit	NN
cord-288669-46tkedw7	363	10	(	(	-LRB-
cord-288669-46tkedw7	363	11	Qiagen	Qiagen	NNP
cord-288669-46tkedw7	363	12	)	)	-RRB-
cord-288669-46tkedw7	363	13	and	and	CC
cord-288669-46tkedw7	363	14	sequenced	sequence	VBN
cord-288669-46tkedw7	363	15	.	.	.
cord-288669-46tkedw7	364	1	Stock	stock	NN
cord-288669-46tkedw7	364	2	solutions	solution	NNS
cord-288669-46tkedw7	364	3	of	of	IN
cord-288669-46tkedw7	364	4	ammonium	ammonium	NN
cord-288669-46tkedw7	364	5	chloride	chloride	NN
cord-288669-46tkedw7	364	6	,	,	,
cord-288669-46tkedw7	364	7	chloroquine	chloroquine	NN
cord-288669-46tkedw7	364	8	,	,	,
cord-288669-46tkedw7	364	9	amantadine	amantadine	NN
cord-288669-46tkedw7	364	10	and	and	CC
cord-288669-46tkedw7	364	11	verapamil	verapamil	NN
cord-288669-46tkedw7	364	12	(	(	-LRB-
cord-288669-46tkedw7	364	13	Sigma	Sigma	NNP
cord-288669-46tkedw7	364	14	)	)	-RRB-
cord-288669-46tkedw7	364	15	were	be	VBD
cord-288669-46tkedw7	364	16	prepared	prepare	VBN
cord-288669-46tkedw7	364	17	in	in	IN
cord-288669-46tkedw7	364	18	water	water	NN
cord-288669-46tkedw7	364	19	at	at	IN
cord-288669-46tkedw7	364	20	concentrations	concentration	NNS
cord-288669-46tkedw7	364	21	of	of	IN
cord-288669-46tkedw7	364	22	50	50	CD
cord-288669-46tkedw7	364	23	mM	mm	CD
cord-288669-46tkedw7	364	24	,	,	,
cord-288669-46tkedw7	364	25	2	2	CD
cord-288669-46tkedw7	364	26	mM	mm	CD
cord-288669-46tkedw7	364	27	,	,	,
cord-288669-46tkedw7	364	28	10	10	CD
cord-288669-46tkedw7	364	29	mM	mm	CD
cord-288669-46tkedw7	364	30	and	and	CC
cord-288669-46tkedw7	364	31	1	1	CD
cord-288669-46tkedw7	364	32	mM	mm	CD
cord-288669-46tkedw7	364	33	,	,	,
cord-288669-46tkedw7	364	34	respectively	respectively	RB
cord-288669-46tkedw7	364	35	.	.	.
cord-288669-46tkedw7	365	1	Marc-145	marc-145	DT
cord-288669-46tkedw7	365	2	cells	cell	NNS
cord-288669-46tkedw7	365	3	grown	grow	VBN
cord-288669-46tkedw7	365	4	in	in	IN
cord-288669-46tkedw7	365	5	6-well	6-well	CD
cord-288669-46tkedw7	365	6	plates	plate	NNS
cord-288669-46tkedw7	365	7	were	be	VBD
cord-288669-46tkedw7	365	8	preincubated	preincubate	VBN
cord-288669-46tkedw7	365	9	with	with	IN
cord-288669-46tkedw7	365	10	different	different	JJ
cord-288669-46tkedw7	365	11	concentrations	concentration	NNS
cord-288669-46tkedw7	365	12	of	of	IN
cord-288669-46tkedw7	365	13	the	the	DT
cord-288669-46tkedw7	365	14	reagents	reagent	NNS
cord-288669-46tkedw7	365	15	for	for	IN
cord-288669-46tkedw7	365	16	30	30	CD
cord-288669-46tkedw7	365	17	min	min	NN
cord-288669-46tkedw7	365	18	and	and	CC
cord-288669-46tkedw7	365	19	subsequently	subsequently	RB
cord-288669-46tkedw7	365	20	infected	infect	VBN
cord-288669-46tkedw7	365	21	with	with	IN
cord-288669-46tkedw7	365	22	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	365	23	at	at	IN
cord-288669-46tkedw7	365	24	a	a	DT
cord-288669-46tkedw7	365	25	multiplicity	multiplicity	NN
cord-288669-46tkedw7	365	26	of	of	IN
cord-288669-46tkedw7	365	27	infection	infection	NN
cord-288669-46tkedw7	365	28	(	(	-LRB-
cord-288669-46tkedw7	365	29	MOI	MOI	NNP
cord-288669-46tkedw7	365	30	)	)	-RRB-
cord-288669-46tkedw7	365	31	of	of	IN
cord-288669-46tkedw7	365	32	1	1	CD
cord-288669-46tkedw7	365	33	for	for	IN
cord-288669-46tkedw7	365	34	1	1	CD
cord-288669-46tkedw7	365	35	h	h	NN
cord-288669-46tkedw7	365	36	at	at	IN
cord-288669-46tkedw7	365	37	37	37	CD
cord-288669-46tkedw7	365	38	°	°	NN
cord-288669-46tkedw7	365	39	C	c	NN
cord-288669-46tkedw7	365	40	in	in	IN
cord-288669-46tkedw7	365	41	the	the	DT
cord-288669-46tkedw7	365	42	presence	presence	NN
cord-288669-46tkedw7	365	43	or	or	CC
cord-288669-46tkedw7	365	44	absence	absence	NN
cord-288669-46tkedw7	365	45	of	of	IN
cord-288669-46tkedw7	365	46	the	the	DT
cord-288669-46tkedw7	365	47	drugs	drug	NNS
cord-288669-46tkedw7	365	48	.	.	.
cord-288669-46tkedw7	366	1	The	the	DT
cord-288669-46tkedw7	366	2	virus	virus	NN
cord-288669-46tkedw7	366	3	inoculum	inoculum	NN
cord-288669-46tkedw7	366	4	was	be	VBD
cord-288669-46tkedw7	366	5	removed	remove	VBN
cord-288669-46tkedw7	366	6	and	and	CC
cord-288669-46tkedw7	366	7	the	the	DT
cord-288669-46tkedw7	366	8	cells	cell	NNS
cord-288669-46tkedw7	366	9	washed	wash	VBD
cord-288669-46tkedw7	366	10	three	three	CD
cord-288669-46tkedw7	366	11	times	time	NNS
cord-288669-46tkedw7	366	12	with	with	IN
cord-288669-46tkedw7	366	13	MEM	MEM	NNP
cord-288669-46tkedw7	366	14	.	.	.
cord-288669-46tkedw7	367	1	The	the	DT
cord-288669-46tkedw7	367	2	inoculated	inoculate	VBN
cord-288669-46tkedw7	367	3	cells	cell	NNS
cord-288669-46tkedw7	367	4	were	be	VBD
cord-288669-46tkedw7	367	5	then	then	RB
cord-288669-46tkedw7	367	6	incubated	incubate	VBN
cord-288669-46tkedw7	367	7	in	in	IN
cord-288669-46tkedw7	367	8	fresh	fresh	JJ
cord-288669-46tkedw7	367	9	medium	medium	NN
cord-288669-46tkedw7	367	10	in	in	IN
cord-288669-46tkedw7	367	11	the	the	DT
cord-288669-46tkedw7	367	12	presence	presence	NN
cord-288669-46tkedw7	367	13	or	or	CC
cord-288669-46tkedw7	367	14	absence	absence	NN
cord-288669-46tkedw7	367	15	of	of	IN
cord-288669-46tkedw7	367	16	the	the	DT
cord-288669-46tkedw7	367	17	reagents	reagent	NNS
cord-288669-46tkedw7	367	18	and	and	CC
cord-288669-46tkedw7	367	19	monitored	monitor	VBD
cord-288669-46tkedw7	367	20	daily	daily	RB
cord-288669-46tkedw7	367	21	for	for	IN
cord-288669-46tkedw7	367	22	the	the	DT
cord-288669-46tkedw7	367	23	appearance	appearance	NN
cord-288669-46tkedw7	367	24	of	of	IN
cord-288669-46tkedw7	367	25	CPE	CPE	NNP
cord-288669-46tkedw7	367	26	.	.	.
cord-288669-46tkedw7	368	1	Culture	culture	NN
cord-288669-46tkedw7	368	2	supernatants	supernatant	NNS
cord-288669-46tkedw7	368	3	were	be	VBD
cord-288669-46tkedw7	368	4	harvested	harvest	VBN
cord-288669-46tkedw7	368	5	daily	daily	RB
cord-288669-46tkedw7	368	6	from	from	IN
cord-288669-46tkedw7	368	7	cells	cell	NNS
cord-288669-46tkedw7	368	8	for	for	IN
cord-288669-46tkedw7	368	9	3	3	CD
cord-288669-46tkedw7	368	10	days	day	NNS
cord-288669-46tkedw7	368	11	and	and	CC
cord-288669-46tkedw7	368	12	virus	virus	NN
cord-288669-46tkedw7	368	13	titer	titer	NN
cord-288669-46tkedw7	368	14	in	in	IN
cord-288669-46tkedw7	368	15	the	the	DT
cord-288669-46tkedw7	368	16	supernatant	supernatant	NN
cord-288669-46tkedw7	368	17	was	be	VBD
cord-288669-46tkedw7	368	18	determined	determine	VBN
cord-288669-46tkedw7	368	19	by	by	IN
cord-288669-46tkedw7	368	20	plaque	plaque	NN
cord-288669-46tkedw7	368	21	assay	assay	NN
cord-288669-46tkedw7	368	22	.	.	.
cord-288669-46tkedw7	369	1	At	at	IN
cord-288669-46tkedw7	369	2	1	1	CD
cord-288669-46tkedw7	369	3	day	day	NN
cord-288669-46tkedw7	369	4	or	or	CC
cord-288669-46tkedw7	369	5	2	2	CD
cord-288669-46tkedw7	369	6	days	day	NNS
cord-288669-46tkedw7	369	7	post	post	NN
cord-288669-46tkedw7	369	8	-	-	NN
cord-288669-46tkedw7	369	9	infection	infection	NN
cord-288669-46tkedw7	369	10	,	,	,
cord-288669-46tkedw7	369	11	Marc-145	marc-145	NN
cord-288669-46tkedw7	369	12	cells	cell	NNS
cord-288669-46tkedw7	369	13	infected	infect	VBN
cord-288669-46tkedw7	369	14	with	with	IN
cord-288669-46tkedw7	369	15	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	369	16	in	in	IN
cord-288669-46tkedw7	369	17	presence	presence	NN
cord-288669-46tkedw7	369	18	or	or	CC
cord-288669-46tkedw7	369	19	absence	absence	NN
cord-288669-46tkedw7	369	20	of	of	IN
cord-288669-46tkedw7	369	21	the	the	DT
cord-288669-46tkedw7	369	22	drugs	drug	NNS
cord-288669-46tkedw7	369	23	were	be	VBD
cord-288669-46tkedw7	369	24	fixed	fix	VBN
cord-288669-46tkedw7	369	25	with	with	IN
cord-288669-46tkedw7	369	26	cold	cold	JJ
cord-288669-46tkedw7	369	27	methanol	methanol	NN
cord-288669-46tkedw7	369	28	and	and	CC
cord-288669-46tkedw7	369	29	subjected	subject	VBN
cord-288669-46tkedw7	369	30	to	to	IN
cord-288669-46tkedw7	369	31	an	an	DT
cord-288669-46tkedw7	369	32	immunofluorescence	immunofluorescence	JJ
cord-288669-46tkedw7	369	33	assay	assay	NN
cord-288669-46tkedw7	369	34	as	as	IN
cord-288669-46tkedw7	369	35	described	describe	VBN
cord-288669-46tkedw7	369	36	above	above	RB
cord-288669-46tkedw7	369	37	.	.	.
cord-288669-46tkedw7	370	1	The	the	DT
cord-288669-46tkedw7	370	2	effect	effect	NN
cord-288669-46tkedw7	370	3	of	of	IN
cord-288669-46tkedw7	370	4	ion	ion	NN
cord-288669-46tkedw7	370	5	channel	channel	NN
cord-288669-46tkedw7	370	6	blockers	blocker	NNS
cord-288669-46tkedw7	370	7	on	on	IN
cord-288669-46tkedw7	370	8	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	370	9	replication	replication	NN
cord-288669-46tkedw7	370	10	was	be	VBD
cord-288669-46tkedw7	370	11	also	also	RB
cord-288669-46tkedw7	370	12	determined	determine	VBN
cord-288669-46tkedw7	370	13	by	by	IN
cord-288669-46tkedw7	370	14	strand	strand	NN
cord-288669-46tkedw7	370	15	-	-	HYPH
cord-288669-46tkedw7	370	16	specific	specific	JJ
cord-288669-46tkedw7	370	17	RT	RT	NNP
cord-288669-46tkedw7	370	18	-	-	HYPH
cord-288669-46tkedw7	370	19	PCR	PCR	NNP
cord-288669-46tkedw7	370	20	.	.	.
cord-288669-46tkedw7	371	1	Total	total	JJ
cord-288669-46tkedw7	371	2	cellular	cellular	JJ
cord-288669-46tkedw7	371	3	RNA	RNA	NNP
cord-288669-46tkedw7	371	4	was	be	VBD
cord-288669-46tkedw7	371	5	extracted	extract	VBN
cord-288669-46tkedw7	371	6	from	from	IN
cord-288669-46tkedw7	371	7	mock	mock	NN
cord-288669-46tkedw7	371	8	-	-	HYPH
cord-288669-46tkedw7	371	9	infected	infect	VBN
cord-288669-46tkedw7	371	10	or	or	CC
cord-288669-46tkedw7	371	11	PRRSV	prrsv	NN
cord-288669-46tkedw7	371	12	-	-	HYPH
cord-288669-46tkedw7	371	13	infected	infect	VBN
cord-288669-46tkedw7	371	14	cells	cell	NNS
cord-288669-46tkedw7	371	15	in	in	IN
cord-288669-46tkedw7	371	16	presence	presence	NN
cord-288669-46tkedw7	371	17	or	or	CC
cord-288669-46tkedw7	371	18	absence	absence	NN
cord-288669-46tkedw7	371	19	of	of	IN
cord-288669-46tkedw7	371	20	the	the	DT
cord-288669-46tkedw7	371	21	drugs	drug	NNS
cord-288669-46tkedw7	371	22	using	use	VBG
cord-288669-46tkedw7	371	23	TRIzol	TRIzol	NNP
cord-288669-46tkedw7	371	24	(	(	-LRB-
cord-288669-46tkedw7	371	25	Invitrogen	Invitrogen	NNP
cord-288669-46tkedw7	371	26	)	)	-RRB-
cord-288669-46tkedw7	371	27	and	and	CC
cord-288669-46tkedw7	371	28	DNA	dna	NN
cord-288669-46tkedw7	371	29	fragments	fragment	NNS
cord-288669-46tkedw7	371	30	representing	represent	VBG
cord-288669-46tkedw7	371	31	positivesense	positivesense	NN
cord-288669-46tkedw7	371	32	or	or	CC
cord-288669-46tkedw7	371	33	negative	negative	JJ
cord-288669-46tkedw7	371	34	-	-	HYPH
cord-288669-46tkedw7	371	35	sense	sense	NN
cord-288669-46tkedw7	371	36	viral	viral	JJ
cord-288669-46tkedw7	371	37	genomic	genomic	JJ
cord-288669-46tkedw7	371	38	RNA	RNA	NNP
cord-288669-46tkedw7	371	39	were	be	VBD
cord-288669-46tkedw7	371	40	RT	RT	NNP
cord-288669-46tkedw7	371	41	-	-	HYPH
cord-288669-46tkedw7	371	42	PCR	PCR	NNP
cord-288669-46tkedw7	371	43	amplified	amplify	VBD
cord-288669-46tkedw7	371	44	using	use	VBG
cord-288669-46tkedw7	371	45	ORF7-specific	ORF7-specific	NNP
cord-288669-46tkedw7	371	46	primers	primer	NNS
cord-288669-46tkedw7	371	47	(	(	-LRB-
cord-288669-46tkedw7	371	48	Lee	Lee	NNP
cord-288669-46tkedw7	371	49	et	et	NNP
cord-288669-46tkedw7	371	50	al	al	NNP
cord-288669-46tkedw7	371	51	.	.	NNP
cord-288669-46tkedw7	371	52	,	,	,
cord-288669-46tkedw7	371	53	2006	2006	CD
cord-288669-46tkedw7	371	54	)	)	-RRB-
cord-288669-46tkedw7	371	55	or	or	CC
cord-288669-46tkedw7	371	56	negative	negative	JJ
cord-288669-46tkedw7	371	57	-	-	HYPH
cord-288669-46tkedw7	371	58	sense	sense	NN
cord-288669-46tkedw7	371	59	-	-	HYPH
cord-288669-46tkedw7	371	60	specific	specific	JJ
cord-288669-46tkedw7	371	61	primers	primer	NNS
cord-288669-46tkedw7	371	62	(	(	-LRB-
cord-288669-46tkedw7	371	63	Table	table	NN
cord-288669-46tkedw7	371	64	1	1	CD
cord-288669-46tkedw7	371	65	)	)	-RRB-
cord-288669-46tkedw7	371	66	,	,	,
cord-288669-46tkedw7	371	67	respectively	respectively	RB
cord-288669-46tkedw7	371	68	,	,	,
cord-288669-46tkedw7	371	69	as	as	IN
cord-288669-46tkedw7	371	70	described	describe	VBN
cord-288669-46tkedw7	371	71	above	above	RB
cord-288669-46tkedw7	371	72	.	.	.
cord-288669-46tkedw7	372	1	An	an	DT
cord-288669-46tkedw7	372	2	inducible	inducible	JJ
cord-288669-46tkedw7	372	3	E.	E.	NNP
cord-288669-46tkedw7	372	4	coli	coli	NN
cord-288669-46tkedw7	372	5	expression	expression	NN
cord-288669-46tkedw7	372	6	system	system	NN
cord-288669-46tkedw7	372	7	was	be	VBD
cord-288669-46tkedw7	372	8	used	use	VBN
cord-288669-46tkedw7	372	9	to	to	TO
cord-288669-46tkedw7	372	10	express	express	VB
cord-288669-46tkedw7	372	11	the	the	DT
cord-288669-46tkedw7	372	12	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	372	13	E	E	NNP
cord-288669-46tkedw7	372	14	protein	protein	NN
cord-288669-46tkedw7	372	15	fused	fuse	VBN
cord-288669-46tkedw7	372	16	with	with	IN
cord-288669-46tkedw7	372	17	glutathione	glutathione	NNP
cord-288669-46tkedw7	372	18	S	s	NN
cord-288669-46tkedw7	372	19	-	-	HYPH
cord-288669-46tkedw7	372	20	transfererase	transfererase	NNP
cord-288669-46tkedw7	372	21	(	(	-LRB-
cord-288669-46tkedw7	372	22	GST	GST	NNP
cord-288669-46tkedw7	372	23	)	)	-RRB-
cord-288669-46tkedw7	372	24	.	.	.
cord-288669-46tkedw7	373	1	Plasmids	plasmid	NNS
cord-288669-46tkedw7	373	2	were	be	VBD
cord-288669-46tkedw7	373	3	transformed	transform	VBN
cord-288669-46tkedw7	373	4	into	into	IN
cord-288669-46tkedw7	373	5	E.	E.	NNP
cord-288669-46tkedw7	373	6	coli	coli	NNS
cord-288669-46tkedw7	373	7	strain	strain	VBP
cord-288669-46tkedw7	373	8	DH5α	DH5α	NNP
cord-288669-46tkedw7	373	9	to	to	TO
cord-288669-46tkedw7	373	10	express	express	VB
cord-288669-46tkedw7	373	11	GST	gst	NN
cord-288669-46tkedw7	373	12	fusion	fusion	NN
cord-288669-46tkedw7	373	13	proteins	protein	NNS
cord-288669-46tkedw7	373	14	.	.	.
cord-288669-46tkedw7	374	1	A	a	DT
cord-288669-46tkedw7	374	2	single	single	JJ
cord-288669-46tkedw7	374	3	colony	colony	NN
cord-288669-46tkedw7	374	4	was	be	VBD
cord-288669-46tkedw7	374	5	grown	grow	VBN
cord-288669-46tkedw7	374	6	in	in	IN
cord-288669-46tkedw7	374	7	Luria	Luria	NNP
cord-288669-46tkedw7	374	8	Bertani	Bertani	NNP
cord-288669-46tkedw7	374	9	(	(	-LRB-
cord-288669-46tkedw7	374	10	LB	LB	NNP
cord-288669-46tkedw7	374	11	)	)	-RRB-
cord-288669-46tkedw7	374	12	media	medium	NNS
cord-288669-46tkedw7	374	13	containing	contain	VBG
cord-288669-46tkedw7	374	14	100	100	CD
cord-288669-46tkedw7	374	15	μg	μg	NNS
cord-288669-46tkedw7	374	16	/	/	SYM
cord-288669-46tkedw7	374	17	ml	ml	NN
cord-288669-46tkedw7	374	18	ampicillin	ampicillin	NN
cord-288669-46tkedw7	374	19	overnight	overnight	RB
cord-288669-46tkedw7	374	20	.	.	.
cord-288669-46tkedw7	375	1	One	one	CD
cord-288669-46tkedw7	375	2	hundred	hundred	CD
cord-288669-46tkedw7	375	3	ml	ml	NNS
cord-288669-46tkedw7	375	4	of	of	IN
cord-288669-46tkedw7	375	5	LB	LB	NNP
cord-288669-46tkedw7	375	6	media	medium	NNS
cord-288669-46tkedw7	375	7	containing	contain	VBG
cord-288669-46tkedw7	375	8	100	100	CD
cord-288669-46tkedw7	375	9	μg	μg	NNS
cord-288669-46tkedw7	375	10	/	/	SYM
cord-288669-46tkedw7	375	11	ml	ml	NN
cord-288669-46tkedw7	376	1	ampicillin	ampicillin	NN
cord-288669-46tkedw7	376	2	was	be	VBD
cord-288669-46tkedw7	376	3	inoculated	inoculate	VBN
cord-288669-46tkedw7	376	4	with	with	IN
cord-288669-46tkedw7	376	5	1/100	1/100	CD
cord-288669-46tkedw7	376	6	of	of	IN
cord-288669-46tkedw7	376	7	overnight	overnight	JJ
cord-288669-46tkedw7	376	8	culture	culture	NN
cord-288669-46tkedw7	376	9	.	.	.
cord-288669-46tkedw7	377	1	When	when	WRB
cord-288669-46tkedw7	377	2	the	the	DT
cord-288669-46tkedw7	377	3	absorbance	absorbance	NN
cord-288669-46tkedw7	377	4	of	of	IN
cord-288669-46tkedw7	377	5	cultures	culture	NNS
cord-288669-46tkedw7	377	6	reached	reach	VBD
cord-288669-46tkedw7	377	7	at	at	IN
cord-288669-46tkedw7	377	8	an	an	DT
cord-288669-46tkedw7	377	9	OD	OD	NNP
cord-288669-46tkedw7	377	10	600	600	CD
cord-288669-46tkedw7	377	11	of	of	IN
cord-288669-46tkedw7	377	12	0.6	0.6	CD
cord-288669-46tkedw7	377	13	,	,	,
cord-288669-46tkedw7	377	14	1	1	CD
cord-288669-46tkedw7	377	15	mM	mm	CD
cord-288669-46tkedw7	377	16	isopropylthio	isopropylthio	NNP
cord-288669-46tkedw7	377	17	-	-	HYPH
cord-288669-46tkedw7	377	18	β	β	NN
cord-288669-46tkedw7	377	19	-	-	HYPH
cord-288669-46tkedw7	377	20	D	D	NNP
cord-288669-46tkedw7	377	21	-	-	HYPH
cord-288669-46tkedw7	377	22	galactoside	galactoside	NN
cord-288669-46tkedw7	377	23	(	(	-LRB-
cord-288669-46tkedw7	377	24	IPTG	IPTG	NNP
cord-288669-46tkedw7	377	25	)	)	-RRB-
cord-288669-46tkedw7	377	26	was	be	VBD
cord-288669-46tkedw7	377	27	added	add	VBN
cord-288669-46tkedw7	377	28	to	to	IN
cord-288669-46tkedw7	377	29	the	the	DT
cord-288669-46tkedw7	377	30	media	medium	NNS
cord-288669-46tkedw7	377	31	to	to	TO
cord-288669-46tkedw7	377	32	induce	induce	VB
cord-288669-46tkedw7	377	33	protein	protein	NN
cord-288669-46tkedw7	377	34	synthesis	synthesis	NN
cord-288669-46tkedw7	377	35	.	.	.
cord-288669-46tkedw7	378	1	At	at	IN
cord-288669-46tkedw7	378	2	indicated	indicate	VBN
cord-288669-46tkedw7	378	3	times	time	NNS
cord-288669-46tkedw7	378	4	after	after	IN
cord-288669-46tkedw7	378	5	induction	induction	NN
cord-288669-46tkedw7	378	6	,	,	,
cord-288669-46tkedw7	378	7	the	the	DT
cord-288669-46tkedw7	378	8	densities	density	NNS
cord-288669-46tkedw7	378	9	of	of	IN
cord-288669-46tkedw7	378	10	bacterial	bacterial	JJ
cord-288669-46tkedw7	378	11	cultures	culture	NNS
cord-288669-46tkedw7	378	12	were	be	VBD
cord-288669-46tkedw7	378	13	determined	determine	VBN
cord-288669-46tkedw7	378	14	by	by	IN
cord-288669-46tkedw7	378	15	measuring	measure	VBG
cord-288669-46tkedw7	378	16	the	the	DT
cord-288669-46tkedw7	378	17	light	light	JJ
cord-288669-46tkedw7	378	18	scattering	scattering	NN
cord-288669-46tkedw7	378	19	at	at	IN
cord-288669-46tkedw7	378	20	600	600	CD
cord-288669-46tkedw7	378	21	nm	nm	NN
cord-288669-46tkedw7	378	22	.	.	.
cord-288669-46tkedw7	379	1	Permeability	permeability	NN
cord-288669-46tkedw7	379	2	of	of	IN
cord-288669-46tkedw7	379	3	the	the	DT
cord-288669-46tkedw7	379	4	plasma	plasma	NN
cord-288669-46tkedw7	379	5	membrane	membrane	NN
cord-288669-46tkedw7	379	6	of	of	IN
cord-288669-46tkedw7	379	7	bacterial	bacterial	JJ
cord-288669-46tkedw7	379	8	cells	cell	NNS
cord-288669-46tkedw7	379	9	expressing	express	VBG
cord-288669-46tkedw7	379	10	the	the	DT
cord-288669-46tkedw7	379	11	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	379	12	E	E	NNP
cord-288669-46tkedw7	379	13	protein	protein	NN
cord-288669-46tkedw7	379	14	to	to	IN
cord-288669-46tkedw7	379	15	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	379	16	B	B	NNP
cord-288669-46tkedw7	379	17	was	be	VBD
cord-288669-46tkedw7	379	18	determined	determine	VBN
cord-288669-46tkedw7	379	19	as	as	IN
cord-288669-46tkedw7	379	20	described	describe	VBN
cord-288669-46tkedw7	379	21	previously	previously	RB
cord-288669-46tkedw7	379	22	(	(	-LRB-
cord-288669-46tkedw7	379	23	Liao	Liao	NNP
cord-288669-46tkedw7	379	24	et	et	FW
cord-288669-46tkedw7	379	25	al	al	NNP
cord-288669-46tkedw7	379	26	.	.	NNP
cord-288669-46tkedw7	379	27	,	,	,
cord-288669-46tkedw7	379	28	2004	2004	CD
cord-288669-46tkedw7	379	29	)	)	-RRB-
cord-288669-46tkedw7	379	30	.	.	.
cord-288669-46tkedw7	380	1	Briefly	briefly	RB
cord-288669-46tkedw7	380	2	,	,	,
cord-288669-46tkedw7	380	3	bacterial	bacterial	JJ
cord-288669-46tkedw7	380	4	cultures	culture	NNS
cord-288669-46tkedw7	380	5	were	be	VBD
cord-288669-46tkedw7	380	6	incubated	incubate	VBN
cord-288669-46tkedw7	380	7	in	in	IN
cord-288669-46tkedw7	380	8	the	the	DT
cord-288669-46tkedw7	380	9	presence	presence	NN
cord-288669-46tkedw7	380	10	or	or	CC
cord-288669-46tkedw7	380	11	absence	absence	NN
cord-288669-46tkedw7	380	12	of	of	IN
cord-288669-46tkedw7	380	13	1	1	CD
cord-288669-46tkedw7	380	14	mM	mm	CD
cord-288669-46tkedw7	380	15	of	of	IN
cord-288669-46tkedw7	380	16	hygromycin	hygromycin	NNS
cord-288669-46tkedw7	380	17	B	B	NNP
cord-288669-46tkedw7	380	18	for	for	IN
cord-288669-46tkedw7	380	19	15	15	CD
cord-288669-46tkedw7	380	20	min	min	NN
cord-288669-46tkedw7	380	21	at	at	IN
cord-288669-46tkedw7	380	22	1	1	CD
cord-288669-46tkedw7	380	23	h	h	NN
cord-288669-46tkedw7	380	24	after	after	IN
cord-288669-46tkedw7	380	25	IPTG	IPTG	NNP
cord-288669-46tkedw7	380	26	induction	induction	NN
cord-288669-46tkedw7	380	27	,	,	,
cord-288669-46tkedw7	380	28	and	and	CC
cord-288669-46tkedw7	380	29	aliquots	aliquot	NNS
cord-288669-46tkedw7	380	30	of	of	IN
cord-288669-46tkedw7	380	31	l	l	NN
cord-288669-46tkedw7	380	32	ml	ml	NNS
cord-288669-46tkedw7	380	33	were	be	VBD
cord-288669-46tkedw7	380	34	labeled	label	VBN
cord-288669-46tkedw7	380	35	with	with	IN
cord-288669-46tkedw7	380	36	for	for	IN
cord-288669-46tkedw7	380	37	15	15	CD
cord-288669-46tkedw7	380	38	min	min	NN
cord-288669-46tkedw7	380	39	with	with	IN
cord-288669-46tkedw7	380	40	4	4	CD
cord-288669-46tkedw7	380	41	μCi	μCi	NNS
cord-288669-46tkedw7	380	42	/	/	SYM
cord-288669-46tkedw7	380	43	ml	ml	NNS
cord-288669-46tkedw7	380	44	of	of	IN
cord-288669-46tkedw7	380	45	[	[	-LRB-
cord-288669-46tkedw7	380	46	35	35	CD
cord-288669-46tkedw7	380	47	S]methionine	S]methionine	NNP
cord-288669-46tkedw7	380	48	/	/	SYM
cord-288669-46tkedw7	380	49	cysteine	cysteine	NN
cord-288669-46tkedw7	380	50	.	.	.
cord-288669-46tkedw7	381	1	The	the	DT
cord-288669-46tkedw7	381	2	labeled	label	VBN
cord-288669-46tkedw7	381	3	bacterial	bacterial	JJ
cord-288669-46tkedw7	381	4	cells	cell	NNS
cord-288669-46tkedw7	381	5	were	be	VBD
cord-288669-46tkedw7	381	6	then	then	RB
cord-288669-46tkedw7	381	7	harvested	harvest	VBN
cord-288669-46tkedw7	381	8	and	and	CC
cord-288669-46tkedw7	381	9	lysed	lyse	VBN
cord-288669-46tkedw7	381	10	in	in	IN
cord-288669-46tkedw7	381	11	equal	equal	JJ
cord-288669-46tkedw7	381	12	volumes	volume	NNS
cord-288669-46tkedw7	381	13	of	of	IN
cord-288669-46tkedw7	381	14	SDS	SDS	NNP
cord-288669-46tkedw7	381	15	-	-	HYPH
cord-288669-46tkedw7	381	16	PAGE	page	NN
cord-288669-46tkedw7	381	17	sample	sample	NN
cord-288669-46tkedw7	381	18	buffer	buffer	NN
cord-288669-46tkedw7	381	19	.	.	.
cord-288669-46tkedw7	382	1	Proteins	protein	NNS
cord-288669-46tkedw7	382	2	were	be	VBD
cord-288669-46tkedw7	382	3	resolved	resolve	VBN
cord-288669-46tkedw7	382	4	on	on	IN
cord-288669-46tkedw7	382	5	a	a	DT
cord-288669-46tkedw7	382	6	12	12	CD
cord-288669-46tkedw7	382	7	%	%	NN
cord-288669-46tkedw7	382	8	SDS	sds	NN
cord-288669-46tkedw7	382	9	-	-	HYPH
cord-288669-46tkedw7	382	10	PAGE	PAGE	NNP
cord-288669-46tkedw7	382	11	gel	gel	NN
cord-288669-46tkedw7	382	12	and	and	CC
cord-288669-46tkedw7	382	13	visualized	visualize	VBN
cord-288669-46tkedw7	382	14	by	by	IN
cord-288669-46tkedw7	382	15	Coomassie	Coomassie	NNP
cord-288669-46tkedw7	382	16	blue	blue	JJ
cord-288669-46tkedw7	382	17	staining	staining	NN
cord-288669-46tkedw7	382	18	or	or	CC
cord-288669-46tkedw7	382	19	autoradiography	autoradiography	NN
cord-288669-46tkedw7	382	20	.	.	.
cord-288669-46tkedw7	383	1	The	the	DT
cord-288669-46tkedw7	383	2	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	383	3	N	N	NNP
cord-288669-46tkedw7	383	4	or	or	CC
cord-288669-46tkedw7	384	1	E	E	NNP
cord-288669-46tkedw7	384	2	protein	protein	NN
cord-288669-46tkedw7	384	3	was	be	VBD
cord-288669-46tkedw7	384	4	independently	independently	RB
cord-288669-46tkedw7	384	5	expressed	express	VBN
cord-288669-46tkedw7	384	6	in	in	IN
cord-288669-46tkedw7	384	7	HeLa	HeLa	NNP
cord-288669-46tkedw7	384	8	cells	cell	NNS
cord-288669-46tkedw7	384	9	using	use	VBG
cord-288669-46tkedw7	384	10	the	the	DT
cord-288669-46tkedw7	384	11	T7-based	t7-base	VBN
cord-288669-46tkedw7	384	12	vaccinia	vaccinia	NN
cord-288669-46tkedw7	384	13	virus	virus	NN
cord-288669-46tkedw7	384	14	vTF7	vTF7	NNP
cord-288669-46tkedw7	384	15	-	-	HYPH
cord-288669-46tkedw7	384	16	3	3	CD
cord-288669-46tkedw7	384	17	.	.	.
cord-288669-46tkedw7	385	1	HeLa	HeLa	NNP
cord-288669-46tkedw7	385	2	cells	cell	NNS
cord-288669-46tkedw7	385	3	seeded	seed	VBN
cord-288669-46tkedw7	385	4	on	on	IN
cord-288669-46tkedw7	385	5	60-mm	60-mm	CD
cord-288669-46tkedw7	385	6	-	-	HYPH
cord-288669-46tkedw7	385	7	diameter	diameter	NN
cord-288669-46tkedw7	385	8	dishes	dish	NNS
cord-288669-46tkedw7	385	9	were	be	VBD
cord-288669-46tkedw7	385	10	grown	grow	VBN
cord-288669-46tkedw7	385	11	to	to	IN
cord-288669-46tkedw7	385	12	90	90	CD
cord-288669-46tkedw7	385	13	%	%	NN
cord-288669-46tkedw7	385	14	confluence	confluence	NN
cord-288669-46tkedw7	385	15	and	and	CC
cord-288669-46tkedw7	385	16	infected	infect	VBN
cord-288669-46tkedw7	385	17	for	for	IN
cord-288669-46tkedw7	385	18	1	1	CD
cord-288669-46tkedw7	385	19	h	h	NN
cord-288669-46tkedw7	385	20	at	at	IN
cord-288669-46tkedw7	385	21	37	37	CD
cord-288669-46tkedw7	385	22	°	°	NN
cord-288669-46tkedw7	385	23	C	c	NN
cord-288669-46tkedw7	385	24	with	with	IN
cord-288669-46tkedw7	385	25	vTF7	vTF7	NNP
cord-288669-46tkedw7	385	26	-	-	HYPH
cord-288669-46tkedw7	385	27	3	3	CD
cord-288669-46tkedw7	385	28	at	at	IN
cord-288669-46tkedw7	385	29	an	an	DT
cord-288669-46tkedw7	385	30	MOI	MOI	NNP
cord-288669-46tkedw7	385	31	of	of	IN
cord-288669-46tkedw7	385	32	10	10	CD
cord-288669-46tkedw7	385	33	.	.	.
cord-288669-46tkedw7	386	1	Following	follow	VBG
cord-288669-46tkedw7	386	2	infection	infection	NN
cord-288669-46tkedw7	386	3	,	,	,
cord-288669-46tkedw7	386	4	fresh	fresh	JJ
cord-288669-46tkedw7	386	5	medium	medium	NN
cord-288669-46tkedw7	386	6	was	be	VBD
cord-288669-46tkedw7	386	7	added	add	VBN
cord-288669-46tkedw7	386	8	and	and	CC
cord-288669-46tkedw7	386	9	incubation	incubation	NN
cord-288669-46tkedw7	386	10	continued	continue	VBD
cord-288669-46tkedw7	386	11	for	for	IN
cord-288669-46tkedw7	386	12	an	an	DT
cord-288669-46tkedw7	386	13	additional	additional	JJ
cord-288669-46tkedw7	386	14	1	1	CD
cord-288669-46tkedw7	386	15	h.	h.	NN
cord-288669-46tkedw7	387	1	The	the	DT
cord-288669-46tkedw7	387	2	cells	cell	NNS
cord-288669-46tkedw7	387	3	were	be	VBD
cord-288669-46tkedw7	387	4	washed	wash	VBN
cord-288669-46tkedw7	387	5	in	in	IN
cord-288669-46tkedw7	387	6	OPTI	OPTI	NNP
cord-288669-46tkedw7	387	7	-	-	HYPH
cord-288669-46tkedw7	387	8	MEM	mem	NN
cord-288669-46tkedw7	387	9	and	and	CC
cord-288669-46tkedw7	387	10	transfected	transfecte	VBN
cord-288669-46tkedw7	387	11	with	with	IN
cord-288669-46tkedw7	387	12	1.5	1.5	CD
cord-288669-46tkedw7	387	13	μg	μg	NNS
cord-288669-46tkedw7	387	14	of	of	IN
cord-288669-46tkedw7	387	15	a	a	DT
cord-288669-46tkedw7	387	16	plasmid	plasmid	NN
cord-288669-46tkedw7	387	17	for	for	IN
cord-288669-46tkedw7	387	18	16	16	CD
cord-288669-46tkedw7	387	19	h	h	NN
cord-288669-46tkedw7	387	20	using	use	VBG
cord-288669-46tkedw7	387	21	the	the	DT
cord-288669-46tkedw7	387	22	reagent	reagent	NN
cord-288669-46tkedw7	387	23	Lipofectin	Lipofectin	NNP
cord-288669-46tkedw7	387	24	.	.	.
cord-288669-46tkedw7	388	1	The	the	DT
cord-288669-46tkedw7	388	2	transfected	transfecte	VBN
cord-288669-46tkedw7	388	3	cells	cell	NNS
cord-288669-46tkedw7	388	4	were	be	VBD
cord-288669-46tkedw7	388	5	then	then	RB
cord-288669-46tkedw7	388	6	starved	starve	VBN
cord-288669-46tkedw7	388	7	for	for	IN
cord-288669-46tkedw7	388	8	30	30	CD
cord-288669-46tkedw7	388	9	min	min	NN
cord-288669-46tkedw7	388	10	in	in	IN
cord-288669-46tkedw7	388	11	methionine	methionine	NN
cord-288669-46tkedw7	388	12	-	-	HYPH
cord-288669-46tkedw7	388	13	deficient	deficient	JJ
cord-288669-46tkedw7	388	14	MEM	mem	NN
cord-288669-46tkedw7	388	15	and	and	CC
cord-288669-46tkedw7	388	16	incubated	incubate	VBN
cord-288669-46tkedw7	388	17	for	for	IN
cord-288669-46tkedw7	388	18	5	5	CD
cord-288669-46tkedw7	388	19	h	h	NN
cord-288669-46tkedw7	388	20	with	with	IN
cord-288669-46tkedw7	388	21	100	100	CD
cord-288669-46tkedw7	388	22	μCi	μCi	NNS
cord-288669-46tkedw7	388	23	/	/	SYM
cord-288669-46tkedw7	388	24	ml	ml	NNS
cord-288669-46tkedw7	388	25	of	of	IN
cord-288669-46tkedw7	388	26	[	[	-LRB-
cord-288669-46tkedw7	388	27	35	35	CD
cord-288669-46tkedw7	388	28	S]methionine	S]methionine	NNP
cord-288669-46tkedw7	388	29	/	/	SYM
cord-288669-46tkedw7	388	30	cysteine	cysteine	NN
cord-288669-46tkedw7	388	31	.	.	.
cord-288669-46tkedw7	389	1	To	to	TO
cord-288669-46tkedw7	389	2	prepare	prepare	VB
cord-288669-46tkedw7	389	3	radiolabeled	radiolabeled	JJ
cord-288669-46tkedw7	389	4	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	389	5	-	-	HYPH
cord-288669-46tkedw7	389	6	infected	infect	VBN
cord-288669-46tkedw7	389	7	cells	cell	NNS
cord-288669-46tkedw7	389	8	,	,	,
cord-288669-46tkedw7	389	9	Marc-145	marc-145	NN
cord-288669-46tkedw7	389	10	cells	cell	NNS
cord-288669-46tkedw7	389	11	seeded	seed	VBN
cord-288669-46tkedw7	389	12	on	on	IN
cord-288669-46tkedw7	389	13	a	a	DT
cord-288669-46tkedw7	389	14	100-mm	100-mm	CD
cord-288669-46tkedw7	389	15	-	-	HYPH
cord-288669-46tkedw7	389	16	diameter	diameter	NN
cord-288669-46tkedw7	389	17	dish	dish	NN
cord-288669-46tkedw7	389	18	were	be	VBD
cord-288669-46tkedw7	389	19	infected	infect	VBN
cord-288669-46tkedw7	389	20	with	with	IN
cord-288669-46tkedw7	389	21	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	389	22	containing	contain	VBG
cord-288669-46tkedw7	389	23	1	1	CD
cord-288669-46tkedw7	389	24	mM	mm	CD
cord-288669-46tkedw7	389	25	PMSF	PMSF	NNP
cord-288669-46tkedw7	389	26	.	.	.
cord-288669-46tkedw7	390	1	The	the	DT
cord-288669-46tkedw7	390	2	resultant	resultant	JJ
cord-288669-46tkedw7	390	3	cell	cell	NN
cord-288669-46tkedw7	390	4	lysates	lysate	NNS
cord-288669-46tkedw7	390	5	were	be	VBD
cord-288669-46tkedw7	390	6	then	then	RB
cord-288669-46tkedw7	390	7	used	use	VBN
cord-288669-46tkedw7	390	8	in	in	IN
cord-288669-46tkedw7	390	9	immunoprecipitation	immunoprecipitation	NN
cord-288669-46tkedw7	390	10	with	with	IN
cord-288669-46tkedw7	390	11	1	1	CD
cord-288669-46tkedw7	390	12	μl	μl	NN
cord-288669-46tkedw7	390	13	of	of	IN
cord-288669-46tkedw7	390	14	the	the	DT
cord-288669-46tkedw7	390	15	N	n	CD
cord-288669-46tkedw7	390	16	-	-	HYPH
cord-288669-46tkedw7	390	17	specific	specific	JJ
cord-288669-46tkedw7	390	18	MAb	MAb	NNS
cord-288669-46tkedw7	390	19	SDOW	SDOW	NNP
cord-288669-46tkedw7	391	1	17	17	CD
cord-288669-46tkedw7	391	2	or	or	CC
cord-288669-46tkedw7	391	3	3	3	CD
cord-288669-46tkedw7	391	4	μl	μl	NN
cord-288669-46tkedw7	391	5	of	of	IN
cord-288669-46tkedw7	391	6	the	the	DT
cord-288669-46tkedw7	391	7	E	e	NN
cord-288669-46tkedw7	391	8	-	-	JJ
cord-288669-46tkedw7	391	9	specific	specific	JJ
cord-288669-46tkedw7	391	10	rabbit	rabbit	NN
cord-288669-46tkedw7	391	11	antiserum	antiserum	NN
cord-288669-46tkedw7	391	12	followed	follow	VBN
cord-288669-46tkedw7	391	13	by	by	IN
cord-288669-46tkedw7	391	14	SDS-17	SDS-17	NNP
cord-288669-46tkedw7	391	15	%	%	NN
cord-288669-46tkedw7	391	16	PAGE	PAGE	NNP
cord-288669-46tkedw7	391	17	analysis	analysis	NN
cord-288669-46tkedw7	391	18	and	and	CC
cord-288669-46tkedw7	391	19	autoradiography	autoradiography	NN
cord-288669-46tkedw7	391	20	as	as	IN
cord-288669-46tkedw7	391	21	described	describe	VBN
cord-288669-46tkedw7	391	22	above	above	RB
cord-288669-46tkedw7	391	23	.	.	.
cord-288669-46tkedw7	392	1	Membrane	membrane	NN
cord-288669-46tkedw7	392	2	permeabilization	permeabilization	NN
cord-288669-46tkedw7	392	3	by	by	IN
cord-288669-46tkedw7	392	4	poliovirus	poliovirus	NN
cord-288669-46tkedw7	392	5	proteins	protein	NNS
cord-288669-46tkedw7	392	6	2B	2b	CD
cord-288669-46tkedw7	392	7	and	and	CC
cord-288669-46tkedw7	392	8	2BC	2BC	NNP
cord-288669-46tkedw7	392	9	Membrane	Membrane	NNP
cord-288669-46tkedw7	392	10	permeabilization	permeabilization	NN
cord-288669-46tkedw7	392	11	by	by	IN
cord-288669-46tkedw7	392	12	different	different	JJ
cord-288669-46tkedw7	392	13	regions	region	NNS
cord-288669-46tkedw7	392	14	of	of	IN
cord-288669-46tkedw7	392	15	the	the	DT
cord-288669-46tkedw7	392	16	human	human	JJ
cord-288669-46tkedw7	392	17	immunodeficiency	immunodeficiency	NN
cord-288669-46tkedw7	392	18	virus	virus	NN
cord-288669-46tkedw7	392	19	type	type	NN
cord-288669-46tkedw7	392	20	1	1	CD
cord-288669-46tkedw7	392	21	transmembrane	transmembrane	NN
cord-288669-46tkedw7	392	22	glycoprotein	glycoprotein	NN
cord-288669-46tkedw7	392	23	gp41	gp41	NN
cord-288669-46tkedw7	392	24	Functional	functional	JJ
cord-288669-46tkedw7	392	25	properties	property	NNS
cord-288669-46tkedw7	392	26	of	of	IN
cord-288669-46tkedw7	392	27	the	the	DT
cord-288669-46tkedw7	392	28	predicted	predict	VBN
cord-288669-46tkedw7	392	29	helicase	helicase	NN
cord-288669-46tkedw7	392	30	of	of	IN
cord-288669-46tkedw7	392	31	porcine	porcine	JJ
cord-288669-46tkedw7	392	32	reproductive	reproductive	JJ
cord-288669-46tkedw7	392	33	and	and	CC
cord-288669-46tkedw7	392	34	respiratory	respiratory	JJ
cord-288669-46tkedw7	392	35	syndrome	syndrome	NN
cord-288669-46tkedw7	392	36	virus	virus	NN
cord-288669-46tkedw7	392	37	Characterization	characterization	NN
cord-288669-46tkedw7	392	38	of	of	IN
cord-288669-46tkedw7	392	39	swine	swine	NN
cord-288669-46tkedw7	392	40	infertility	infertility	NN
cord-288669-46tkedw7	392	41	and	and	CC
cord-288669-46tkedw7	392	42	respiratory	respiratory	JJ
cord-288669-46tkedw7	392	43	syndrome	syndrome	NN
cord-288669-46tkedw7	392	44	(	(	-LRB-
cord-288669-46tkedw7	392	45	SIRS	SIRS	NNP
cord-288669-46tkedw7	392	46	)	)	-RRB-
cord-288669-46tkedw7	392	47	virus	virus	NN
cord-288669-46tkedw7	392	48	(	(	-LRB-
cord-288669-46tkedw7	392	49	isolate	isolate	VB
cord-288669-46tkedw7	392	50	ATCC	ATCC	NNP
cord-288669-46tkedw7	392	51	VR-2332	vr-2332	NN
cord-288669-46tkedw7	392	52	)	)	-RRB-
cord-288669-46tkedw7	393	1	Modification	modification	NN
cord-288669-46tkedw7	393	2	of	of	IN
cord-288669-46tkedw7	393	3	late	late	JJ
cord-288669-46tkedw7	393	4	membrane	membrane	NN
cord-288669-46tkedw7	393	5	permeability	permeability	NN
cord-288669-46tkedw7	393	6	in	in	IN
cord-288669-46tkedw7	393	7	avian	avian	JJ
cord-288669-46tkedw7	393	8	Reovirus	Reovirus	NNP
cord-288669-46tkedw7	393	9	-	-	HYPH
cord-288669-46tkedw7	393	10	infected	infect	VBN
cord-288669-46tkedw7	393	11	cells	cell	NNS
cord-288669-46tkedw7	393	12	Modification	modification	NN
cord-288669-46tkedw7	393	13	of	of	IN
cord-288669-46tkedw7	393	14	membrane	membrane	NN
cord-288669-46tkedw7	393	15	permeability	permeability	NN
cord-288669-46tkedw7	393	16	by	by	IN
cord-288669-46tkedw7	393	17	animal	animal	NN
cord-288669-46tkedw7	393	18	viruses	virus	NNS
cord-288669-46tkedw7	394	1	Membrane	membrane	NN
cord-288669-46tkedw7	394	2	permeabilization	permeabilization	NN
cord-288669-46tkedw7	394	3	by	by	IN
cord-288669-46tkedw7	394	4	small	small	JJ
cord-288669-46tkedw7	394	5	hydrophobic	hydrophobic	JJ
cord-288669-46tkedw7	394	6	nonstructural	nonstructural	JJ
cord-288669-46tkedw7	394	7	proteins	protein	NNS
cord-288669-46tkedw7	394	8	of	of	IN
cord-288669-46tkedw7	394	9	Japanese	japanese	JJ
cord-288669-46tkedw7	394	10	encephalitis	encephalitis	RB
cord-288669-46tkedw7	394	11	virus	virus	NN
cord-288669-46tkedw7	394	12	Hepatitis	Hepatitis	NNP
cord-288669-46tkedw7	394	13	C	C	NNP
cord-288669-46tkedw7	394	14	virus	virus	NN
cord-288669-46tkedw7	394	15	E1	e1	NN
cord-288669-46tkedw7	394	16	protein	protein	NN
cord-288669-46tkedw7	394	17	induces	induce	VBZ
cord-288669-46tkedw7	394	18	modification	modification	NN
cord-288669-46tkedw7	394	19	of	of	IN
cord-288669-46tkedw7	394	20	membrane	membrane	NN
cord-288669-46tkedw7	394	21	permeability	permeability	NN
cord-288669-46tkedw7	394	22	in	in	IN
cord-288669-46tkedw7	394	23	E.	E.	NNP
cord-288669-46tkedw7	394	24	coli	coli	NNS
cord-288669-46tkedw7	394	25	cells	cell	NNS
cord-288669-46tkedw7	394	26	Ultrastructural	ultrastructural	JJ
cord-288669-46tkedw7	394	27	characteristics	characteristic	NNS
cord-288669-46tkedw7	394	28	and	and	CC
cord-288669-46tkedw7	394	29	morphogenesis	morphogenesis	NN
cord-288669-46tkedw7	394	30	of	of	IN
cord-288669-46tkedw7	394	31	porcine	porcine	JJ
cord-288669-46tkedw7	394	32	reproductive	reproductive	JJ
cord-288669-46tkedw7	394	33	and	and	CC
cord-288669-46tkedw7	394	34	respiratory	respiratory	JJ
cord-288669-46tkedw7	394	35	syndrome	syndrome	NN
cord-288669-46tkedw7	394	36	virus	virus	NN
cord-288669-46tkedw7	394	37	propagated	propagate	VBN
cord-288669-46tkedw7	394	38	in	in	IN
cord-288669-46tkedw7	394	39	the	the	DT
cord-288669-46tkedw7	394	40	highly	highly	RB
cord-288669-46tkedw7	394	41	permissive	permissive	JJ
cord-288669-46tkedw7	394	42	MARC-145	MARC-145	NNP
cord-288669-46tkedw7	394	43	cell	cell	NN
cord-288669-46tkedw7	394	44	clone	clone	NN
cord-288669-46tkedw7	394	45	Determinants	determinant	NNS
cord-288669-46tkedw7	394	46	for	for	IN
cord-288669-46tkedw7	394	47	membrane	membrane	NNP
cord-288669-46tkedw7	394	48	association	association	NNP
cord-288669-46tkedw7	394	49	and	and	CC
cord-288669-46tkedw7	394	50	permeabilization	permeabilization	NN
cord-288669-46tkedw7	394	51	of	of	IN
cord-288669-46tkedw7	394	52	the	the	DT
cord-288669-46tkedw7	394	53	coxsackievirus	coxsackievirus	NNP
cord-288669-46tkedw7	394	54	2B	2B	NNP
cord-288669-46tkedw7	394	55	protein	protein	NNP
cord-288669-46tkedw7	394	56	and	and	CC
cord-288669-46tkedw7	394	57	the	the	DT
cord-288669-46tkedw7	394	58	identification	identification	NN
cord-288669-46tkedw7	394	59	of	of	IN
cord-288669-46tkedw7	394	60	the	the	DT
cord-288669-46tkedw7	394	61	Golgi	Golgi	NNP
cord-288669-46tkedw7	394	62	complex	complex	NN
cord-288669-46tkedw7	394	63	as	as	IN
cord-288669-46tkedw7	394	64	the	the	DT
cord-288669-46tkedw7	394	65	target	target	NN
cord-288669-46tkedw7	394	66	organelle	organelle	NN
cord-288669-46tkedw7	394	67	Inhibition	inhibition	NN
cord-288669-46tkedw7	394	68	of	of	IN
cord-288669-46tkedw7	394	69	cellular	cellular	JJ
cord-288669-46tkedw7	394	70	protein	protein	NN
cord-288669-46tkedw7	394	71	secretion	secretion	NN
cord-288669-46tkedw7	394	72	by	by	IN
cord-288669-46tkedw7	394	73	poliovirus	poliovirus	NN
cord-288669-46tkedw7	394	74	proteins	protein	NNS
cord-288669-46tkedw7	394	75	2B	2b	CD
cord-288669-46tkedw7	394	76	and	and	CC
cord-288669-46tkedw7	394	77	3A	3A	NNP
cord-288669-46tkedw7	394	78	Analysis	Analysis	NNP
cord-288669-46tkedw7	394	79	of	of	IN
cord-288669-46tkedw7	394	80	constructed	construct	VBN
cord-288669-46tkedw7	394	81	E	e	NN
cord-288669-46tkedw7	394	82	gene	gene	NN
cord-288669-46tkedw7	394	83	mutants	mutant	NNS
cord-288669-46tkedw7	394	84	of	of	IN
cord-288669-46tkedw7	394	85	mouse	mouse	NNP
cord-288669-46tkedw7	394	86	hepatitis	hepatitis	NNP
cord-288669-46tkedw7	394	87	virus	virus	NNP
cord-288669-46tkedw7	394	88	confirms	confirm	VBZ
cord-288669-46tkedw7	394	89	a	a	DT
cord-288669-46tkedw7	394	90	pivotal	pivotal	JJ
cord-288669-46tkedw7	394	91	role	role	NN
cord-288669-46tkedw7	394	92	for	for	IN
cord-288669-46tkedw7	394	93	E	e	NN
cord-288669-46tkedw7	394	94	protein	protein	NN
cord-288669-46tkedw7	394	95	in	in	IN
cord-288669-46tkedw7	394	96	coronavirus	coronavirus	NN
cord-288669-46tkedw7	394	97	assembly	assembly	NN
cord-288669-46tkedw7	394	98	Viral	viral	JJ
cord-288669-46tkedw7	394	99	ion	ion	NN
cord-288669-46tkedw7	394	100	channels	channel	NNS
cord-288669-46tkedw7	394	101	:	:	:
cord-288669-46tkedw7	394	102	structure	structure	NN
cord-288669-46tkedw7	394	103	and	and	CC
cord-288669-46tkedw7	394	104	function	function	VB
cord-288669-46tkedw7	394	105	Eukaryotic	eukaryotic	JJ
cord-288669-46tkedw7	394	106	transientexpression	transientexpression	NN
cord-288669-46tkedw7	394	107	system	system	NN
cord-288669-46tkedw7	394	108	based	base	VBN
cord-288669-46tkedw7	394	109	on	on	IN
cord-288669-46tkedw7	394	110	recombinant	recombinant	JJ
cord-288669-46tkedw7	394	111	vaccinia	vaccinia	NN
cord-288669-46tkedw7	394	112	virus	virus	NN
cord-288669-46tkedw7	394	113	that	that	WDT
cord-288669-46tkedw7	394	114	synthesizes	synthesize	VBZ
cord-288669-46tkedw7	394	115	bacteriophage	bacteriophage	NN
cord-288669-46tkedw7	394	116	T7	T7	NNP
cord-288669-46tkedw7	394	117	RNA	RNA	NNP
cord-288669-46tkedw7	394	118	polymerase	polymerase	NN
cord-288669-46tkedw7	395	1	The	the	DT
cord-288669-46tkedw7	395	2	NS3	ns3	RB
cord-288669-46tkedw7	395	3	protein	protein	NN
cord-288669-46tkedw7	395	4	of	of	IN
cord-288669-46tkedw7	395	5	bluetongue	bluetongue	NNP
cord-288669-46tkedw7	395	6	virus	virus	NN
cord-288669-46tkedw7	395	7	exhibits	exhibit	VBZ
cord-288669-46tkedw7	395	8	viroporin	viroporin	NN
cord-288669-46tkedw7	395	9	-	-	HYPH
cord-288669-46tkedw7	395	10	like	like	JJ
cord-288669-46tkedw7	395	11	properties	property	NNS
cord-288669-46tkedw7	396	1	Enhanced	enhanced	JJ
cord-288669-46tkedw7	396	2	replication	replication	NN
cord-288669-46tkedw7	396	3	of	of	IN
cord-288669-46tkedw7	396	4	porcine	porcine	JJ
cord-288669-46tkedw7	396	5	reproductive	reproductive	JJ
cord-288669-46tkedw7	396	6	and	and	CC
cord-288669-46tkedw7	396	7	respiratory	respiratory	JJ
cord-288669-46tkedw7	396	8	syndrome	syndrome	NN
cord-288669-46tkedw7	396	9	(	(	-LRB-
cord-288669-46tkedw7	396	10	PRRS	PRRS	NNP
cord-288669-46tkedw7	396	11	)	)	-RRB-
cord-288669-46tkedw7	396	12	virus	virus	NN
cord-288669-46tkedw7	396	13	in	in	IN
cord-288669-46tkedw7	396	14	a	a	DT
cord-288669-46tkedw7	396	15	homogeneous	homogeneous	JJ
cord-288669-46tkedw7	396	16	subpopulation	subpopulation	NN
cord-288669-46tkedw7	396	17	of	of	IN
cord-288669-46tkedw7	396	18	MA-104	MA-104	NNP
cord-288669-46tkedw7	396	19	cell	cell	NN
cord-288669-46tkedw7	396	20	line	line	NN
cord-288669-46tkedw7	396	21	Porcine	porcine	JJ
cord-288669-46tkedw7	396	22	reproductive	reproductive	JJ
cord-288669-46tkedw7	396	23	and	and	CC
cord-288669-46tkedw7	396	24	respiratory	respiratory	JJ
cord-288669-46tkedw7	396	25	syndrome	syndrome	NN
cord-288669-46tkedw7	396	26	virus	virus	NN
cord-288669-46tkedw7	396	27	enters	enter	VBZ
cord-288669-46tkedw7	396	28	cells	cell	NNS
cord-288669-46tkedw7	396	29	through	through	IN
cord-288669-46tkedw7	396	30	a	a	DT
cord-288669-46tkedw7	396	31	low	low	JJ
cord-288669-46tkedw7	396	32	pH	pH	NNP
cord-288669-46tkedw7	396	33	dependent	dependent	JJ
cord-288669-46tkedw7	396	34	endocytic	endocytic	JJ
cord-288669-46tkedw7	396	35	pathway	pathway	NN
cord-288669-46tkedw7	396	36	Cysteine	cysteine	NN
cord-288669-46tkedw7	396	37	residues	residue	NNS
cord-288669-46tkedw7	396	38	of	of	IN
cord-288669-46tkedw7	396	39	the	the	DT
cord-288669-46tkedw7	396	40	porcine	porcine	JJ
cord-288669-46tkedw7	396	41	reproductive	reproductive	JJ
cord-288669-46tkedw7	396	42	and	and	CC
cord-288669-46tkedw7	396	43	respiratory	respiratory	JJ
cord-288669-46tkedw7	396	44	syndrome	syndrome	NN
cord-288669-46tkedw7	396	45	virus	virus	NN
cord-288669-46tkedw7	396	46	small	small	JJ
cord-288669-46tkedw7	396	47	envelope	envelope	NN
cord-288669-46tkedw7	396	48	protein	protein	NN
cord-288669-46tkedw7	396	49	are	be	VBP
cord-288669-46tkedw7	396	50	non	non	JJ
cord-288669-46tkedw7	396	51	-	-	JJ
cord-288669-46tkedw7	396	52	essential	essential	JJ
cord-288669-46tkedw7	396	53	for	for	IN
cord-288669-46tkedw7	396	54	virus	virus	NN
cord-288669-46tkedw7	396	55	infectivity	infectivity	NN
cord-288669-46tkedw7	396	56	A	a	DT
cord-288669-46tkedw7	396	57	DNA	dna	NN
cord-288669-46tkedw7	396	58	-	-	HYPH
cord-288669-46tkedw7	396	59	launched	launch	VBN
cord-288669-46tkedw7	396	60	reverse	reverse	NN
cord-288669-46tkedw7	396	61	genetics	genetics	NN
cord-288669-46tkedw7	396	62	system	system	NN
cord-288669-46tkedw7	396	63	for	for	IN
cord-288669-46tkedw7	396	64	porcine	porcine	JJ
cord-288669-46tkedw7	396	65	reproductive	reproductive	JJ
cord-288669-46tkedw7	396	66	and	and	CC
cord-288669-46tkedw7	396	67	respiratory	respiratory	JJ
cord-288669-46tkedw7	396	68	syndrome	syndrome	NN
cord-288669-46tkedw7	396	69	virus	virus	NN
cord-288669-46tkedw7	396	70	reveals	reveal	VBZ
cord-288669-46tkedw7	396	71	that	that	IN
cord-288669-46tkedw7	396	72	homodimerization	homodimerization	NN
cord-288669-46tkedw7	396	73	of	of	IN
cord-288669-46tkedw7	396	74	the	the	DT
cord-288669-46tkedw7	396	75	nucleocapsid	nucleocapsid	NNP
cord-288669-46tkedw7	396	76	protein	protein	NN
cord-288669-46tkedw7	396	77	is	be	VBZ
cord-288669-46tkedw7	396	78	essential	essential	JJ
cord-288669-46tkedw7	396	79	for	for	IN
cord-288669-46tkedw7	396	80	virus	virus	NN
cord-288669-46tkedw7	396	81	infectivity	infectivity	NN
cord-288669-46tkedw7	396	82	Mutations	Mutations	NNPS
cord-288669-46tkedw7	396	83	within	within	IN
cord-288669-46tkedw7	396	84	the	the	DT
cord-288669-46tkedw7	396	85	nuclear	nuclear	JJ
cord-288669-46tkedw7	396	86	localization	localization	NN
cord-288669-46tkedw7	396	87	signal	signal	NN
cord-288669-46tkedw7	396	88	of	of	IN
cord-288669-46tkedw7	396	89	the	the	DT
cord-288669-46tkedw7	396	90	porcine	porcine	JJ
cord-288669-46tkedw7	396	91	reproductive	reproductive	JJ
cord-288669-46tkedw7	396	92	and	and	CC
cord-288669-46tkedw7	396	93	respiratory	respiratory	JJ
cord-288669-46tkedw7	396	94	syndrome	syndrome	NN
cord-288669-46tkedw7	396	95	virus	virus	NN
cord-288669-46tkedw7	396	96	nucleocapsid	nucleocapsid	NN
cord-288669-46tkedw7	396	97	protein	protein	NN
cord-288669-46tkedw7	396	98	attenuate	attenuate	VBP
cord-288669-46tkedw7	396	99	virus	virus	NN
cord-288669-46tkedw7	396	100	replication	replication	NN
cord-288669-46tkedw7	396	101	Expression	expression	NN
cord-288669-46tkedw7	396	102	of	of	IN
cord-288669-46tkedw7	396	103	SARS	SARS	NNP
cord-288669-46tkedw7	396	104	-	-	HYPH
cord-288669-46tkedw7	396	105	coronavirus	coronavirus	NN
cord-288669-46tkedw7	396	106	envelope	envelope	NN
cord-288669-46tkedw7	396	107	protein	protein	NN
cord-288669-46tkedw7	396	108	in	in	IN
cord-288669-46tkedw7	396	109	Escherichia	Escherichia	NNP
cord-288669-46tkedw7	396	110	coli	coli	NNS
cord-288669-46tkedw7	396	111	alters	alter	VBZ
cord-288669-46tkedw7	396	112	membrane	membrane	NN
cord-288669-46tkedw7	396	113	permeability	permeability	NNP
cord-288669-46tkedw7	396	114	Association	Association	NNP
cord-288669-46tkedw7	396	115	of	of	IN
cord-288669-46tkedw7	396	116	the	the	DT
cord-288669-46tkedw7	396	117	infectious	infectious	JJ
cord-288669-46tkedw7	396	118	bronchitis	bronchitis	NN
cord-288669-46tkedw7	396	119	virus	virus	NN
cord-288669-46tkedw7	396	120	3c	3c	NNP
cord-288669-46tkedw7	396	121	protein	protein	NN
cord-288669-46tkedw7	396	122	with	with	IN
cord-288669-46tkedw7	396	123	the	the	DT
cord-288669-46tkedw7	396	124	virion	virion	NN
cord-288669-46tkedw7	396	125	envelope	envelope	NN
cord-288669-46tkedw7	396	126	Viroporin	Viroporin	NNP
cord-288669-46tkedw7	396	127	activity	activity	NN
cord-288669-46tkedw7	396	128	of	of	IN
cord-288669-46tkedw7	396	129	murine	murine	JJ
cord-288669-46tkedw7	396	130	hepatitis	hepatitis	NN
cord-288669-46tkedw7	396	131	virus	virus	NN
cord-288669-46tkedw7	396	132	E	E	NNP
cord-288669-46tkedw7	396	133	protein	protein	NN
cord-288669-46tkedw7	396	134	Human	human	JJ
cord-288669-46tkedw7	396	135	immunodeficiency	immunodeficiency	NN
cord-288669-46tkedw7	396	136	virus	virus	NN
cord-288669-46tkedw7	396	137	type	type	NN
cord-288669-46tkedw7	397	1	1	1	LS
cord-288669-46tkedw7	397	2	Vpu	Vpu	NNP
cord-288669-46tkedw7	397	3	protein	protein	NN
cord-288669-46tkedw7	397	4	is	be	VBZ
cord-288669-46tkedw7	397	5	an	an	DT
cord-288669-46tkedw7	397	6	oligomeric	oligomeric	JJ
cord-288669-46tkedw7	397	7	type	type	NN
cord-288669-46tkedw7	397	8	I	-PRON-	PRP
cord-288669-46tkedw7	397	9	integral	integral	JJ
cord-288669-46tkedw7	397	10	membrane	membrane	NN
cord-288669-46tkedw7	397	11	protein	protein	NN
cord-288669-46tkedw7	397	12	Phylogenetic	Phylogenetic	NNP
cord-288669-46tkedw7	397	13	analysis	analysis	NN
cord-288669-46tkedw7	397	14	of	of	IN
cord-288669-46tkedw7	397	15	the	the	DT
cord-288669-46tkedw7	397	16	putative	putative	JJ
cord-288669-46tkedw7	397	17	M	M	NNP
cord-288669-46tkedw7	397	18	(	(	-LRB-
cord-288669-46tkedw7	397	19	ORF	ORF	NNP
cord-288669-46tkedw7	397	20	6	6	CD
cord-288669-46tkedw7	397	21	)	)	-RRB-
cord-288669-46tkedw7	398	1	and	and	CC
cord-288669-46tkedw7	398	2	N	N	NNP
cord-288669-46tkedw7	398	3	(	(	-LRB-
cord-288669-46tkedw7	398	4	ORF	ORF	NNP
cord-288669-46tkedw7	398	5	7	7	CD
cord-288669-46tkedw7	398	6	)	)	-RRB-
cord-288669-46tkedw7	398	7	genes	gene	NNS
cord-288669-46tkedw7	398	8	of	of	IN
cord-288669-46tkedw7	398	9	porcine	porcine	JJ
cord-288669-46tkedw7	398	10	reproductive	reproductive	JJ
cord-288669-46tkedw7	398	11	and	and	CC
cord-288669-46tkedw7	398	12	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	13	syndrome	syndrome	NN
cord-288669-46tkedw7	398	14	virus	virus	NN
cord-288669-46tkedw7	398	15	(	(	-LRB-
cord-288669-46tkedw7	398	16	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	398	17	)	)	-RRB-
cord-288669-46tkedw7	398	18	:	:	:
cord-288669-46tkedw7	398	19	implication	implication	NN
cord-288669-46tkedw7	398	20	for	for	IN
cord-288669-46tkedw7	398	21	the	the	DT
cord-288669-46tkedw7	398	22	existence	existence	NN
cord-288669-46tkedw7	398	23	of	of	IN
cord-288669-46tkedw7	398	24	two	two	CD
cord-288669-46tkedw7	398	25	genotypes	genotype	NNS
cord-288669-46tkedw7	398	26	of	of	IN
cord-288669-46tkedw7	398	27	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	398	28	in	in	IN
cord-288669-46tkedw7	398	29	the	the	DT
cord-288669-46tkedw7	398	30	U.S.A.	U.S.A.	NNP
cord-288669-46tkedw7	398	31	and	and	CC
cord-288669-46tkedw7	398	32	Europe	Europe	NNP
cord-288669-46tkedw7	398	33	Lelystad	Lelystad	NNP
cord-288669-46tkedw7	398	34	virus	virus	NN
cord-288669-46tkedw7	398	35	,	,	,
cord-288669-46tkedw7	398	36	the	the	DT
cord-288669-46tkedw7	398	37	causative	causative	JJ
cord-288669-46tkedw7	398	38	agent	agent	NN
cord-288669-46tkedw7	398	39	of	of	IN
cord-288669-46tkedw7	398	40	porcine	porcine	JJ
cord-288669-46tkedw7	398	41	epidemic	epidemic	NN
cord-288669-46tkedw7	398	42	abortion	abortion	NN
cord-288669-46tkedw7	398	43	and	and	CC
cord-288669-46tkedw7	398	44	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	45	syndrome	syndrome	NN
cord-288669-46tkedw7	398	46	(	(	-LRB-
cord-288669-46tkedw7	398	47	PEARS	PEARS	NNP
cord-288669-46tkedw7	398	48	)	)	-RRB-
cord-288669-46tkedw7	398	49	,	,	,
cord-288669-46tkedw7	398	50	is	be	VBZ
cord-288669-46tkedw7	398	51	related	relate	VBN
cord-288669-46tkedw7	398	52	to	to	IN
cord-288669-46tkedw7	398	53	LDV	LDV	NNP
cord-288669-46tkedw7	398	54	and	and	CC
cord-288669-46tkedw7	398	55	EAV	EAV	NNP
cord-288669-46tkedw7	398	56	Characterization	Characterization	NNP
cord-288669-46tkedw7	398	57	of	of	IN
cord-288669-46tkedw7	398	58	proteins	protein	NNS
cord-288669-46tkedw7	398	59	encoded	encode	VBN
cord-288669-46tkedw7	398	60	by	by	IN
cord-288669-46tkedw7	398	61	ORFs	ORFs	NNP
cord-288669-46tkedw7	398	62	2	2	CD
cord-288669-46tkedw7	398	63	to	to	IN
cord-288669-46tkedw7	398	64	7	7	CD
cord-288669-46tkedw7	398	65	of	of	IN
cord-288669-46tkedw7	398	66	Lelystad	Lelystad	NNP
cord-288669-46tkedw7	398	67	virus	virus	NN
cord-288669-46tkedw7	398	68	Entry	entry	NN
cord-288669-46tkedw7	398	69	of	of	IN
cord-288669-46tkedw7	398	70	porcine	porcine	JJ
cord-288669-46tkedw7	398	71	reproductive	reproductive	JJ
cord-288669-46tkedw7	398	72	and	and	CC
cord-288669-46tkedw7	398	73	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	74	syndrome	syndrome	NN
cord-288669-46tkedw7	398	75	virus	virus	NN
cord-288669-46tkedw7	398	76	into	into	IN
cord-288669-46tkedw7	398	77	porcine	porcine	JJ
cord-288669-46tkedw7	398	78	alveolar	alveolar	NN
cord-288669-46tkedw7	398	79	macrophages	macrophage	NNS
cord-288669-46tkedw7	398	80	via	via	IN
cord-288669-46tkedw7	398	81	receptor	receptor	NN
cord-288669-46tkedw7	398	82	-	-	HYPH
cord-288669-46tkedw7	398	83	mediated	mediate	VBN
cord-288669-46tkedw7	398	84	endocytosis	endocytosis	NN
cord-288669-46tkedw7	398	85	Porcine	porcine	JJ
cord-288669-46tkedw7	398	86	reproductive	reproductive	JJ
cord-288669-46tkedw7	398	87	and	and	CC
cord-288669-46tkedw7	398	88	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	89	syndrome	syndrome	NN
cord-288669-46tkedw7	398	90	virus	virus	NN
cord-288669-46tkedw7	398	91	comparison	comparison	NN
cord-288669-46tkedw7	398	92	:	:	:
cord-288669-46tkedw7	398	93	divergent	divergent	JJ
cord-288669-46tkedw7	398	94	evolution	evolution	NN
cord-288669-46tkedw7	398	95	on	on	IN
cord-288669-46tkedw7	398	96	two	two	CD
cord-288669-46tkedw7	398	97	continents	continent	NNS
cord-288669-46tkedw7	398	98	Differentiation	differentiation	NN
cord-288669-46tkedw7	398	99	of	of	IN
cord-288669-46tkedw7	398	100	US	US	NNP
cord-288669-46tkedw7	398	101	and	and	CC
cord-288669-46tkedw7	398	102	European	european	JJ
cord-288669-46tkedw7	398	103	isolates	isolate	NNS
cord-288669-46tkedw7	398	104	of	of	IN
cord-288669-46tkedw7	398	105	porcine	porcine	JJ
cord-288669-46tkedw7	398	106	reproductive	reproductive	JJ
cord-288669-46tkedw7	398	107	and	and	CC
cord-288669-46tkedw7	398	108	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	109	syndrome	syndrome	NN
cord-288669-46tkedw7	398	110	virus	virus	NN
cord-288669-46tkedw7	398	111	by	by	IN
cord-288669-46tkedw7	398	112	monoclonal	monoclonal	JJ
cord-288669-46tkedw7	398	113	antibodies	antibody	NNS
cord-288669-46tkedw7	398	114	Assessment	Assessment	NNP
cord-288669-46tkedw7	398	115	of	of	IN
cord-288669-46tkedw7	398	116	the	the	DT
cord-288669-46tkedw7	398	117	economic	economic	JJ
cord-288669-46tkedw7	398	118	impact	impact	NN
cord-288669-46tkedw7	398	119	of	of	IN
cord-288669-46tkedw7	398	120	porcine	porcine	JJ
cord-288669-46tkedw7	398	121	reproductive	reproductive	JJ
cord-288669-46tkedw7	398	122	and	and	CC
cord-288669-46tkedw7	398	123	respiratory	respiratory	JJ
cord-288669-46tkedw7	398	124	syndrome	syndrome	NN
cord-288669-46tkedw7	398	125	on	on	IN
cord-288669-46tkedw7	398	126	swine	swine	NN
cord-288669-46tkedw7	398	127	production	production	NN
cord-288669-46tkedw7	398	128	in	in	IN
cord-288669-46tkedw7	398	129	the	the	DT
cord-288669-46tkedw7	398	130	United	United	NNP
cord-288669-46tkedw7	398	131	States	States	NNP
cord-288669-46tkedw7	398	132	Influenza	Influenza	NNP
cord-288669-46tkedw7	398	133	B	B	NNP
cord-288669-46tkedw7	398	134	virus	virus	NN
cord-288669-46tkedw7	398	135	BM2	BM2	NNP
cord-288669-46tkedw7	398	136	protein	protein	NN
cord-288669-46tkedw7	398	137	is	be	VBZ
cord-288669-46tkedw7	399	1	an	an	DT
cord-288669-46tkedw7	399	2	oligomeric	oligomeric	JJ
cord-288669-46tkedw7	399	3	integral	integral	JJ
cord-288669-46tkedw7	399	4	membrane	membrane	NN
cord-288669-46tkedw7	399	5	protein	protein	NN
cord-288669-46tkedw7	399	6	expressed	express	VBN
cord-288669-46tkedw7	399	7	at	at	IN
cord-288669-46tkedw7	399	8	the	the	DT
cord-288669-46tkedw7	399	9	cell	cell	NN
cord-288669-46tkedw7	399	10	surface	surface	NN
cord-288669-46tkedw7	399	11	Influenza	Influenza	NNP
cord-288669-46tkedw7	399	12	virus	virus	NN
cord-288669-46tkedw7	399	13	M2	m2	NN
cord-288669-46tkedw7	399	14	protein	protein	NN
cord-288669-46tkedw7	399	15	has	have	VBZ
cord-288669-46tkedw7	399	16	ion	ion	NN
cord-288669-46tkedw7	399	17	channel	channel	NN
cord-288669-46tkedw7	399	18	activity	activity	NN
cord-288669-46tkedw7	400	1	The	the	DT
cord-288669-46tkedw7	400	2	cap	cap	NN
cord-288669-46tkedw7	400	3	structure	structure	NN
cord-288669-46tkedw7	400	4	of	of	IN
cord-288669-46tkedw7	400	5	simian	simian	JJ
cord-288669-46tkedw7	400	6	hemorrhagic	hemorrhagic	JJ
cord-288669-46tkedw7	400	7	fever	fever	NN
cord-288669-46tkedw7	400	8	virion	virion	NN
cord-288669-46tkedw7	400	9	RNA	rna	NN
cord-288669-46tkedw7	401	1	The	the	DT
cord-288669-46tkedw7	401	2	active	active	JJ
cord-288669-46tkedw7	401	3	oligomeric	oligomeric	JJ
cord-288669-46tkedw7	401	4	state	state	NN
cord-288669-46tkedw7	401	5	of	of	IN
cord-288669-46tkedw7	401	6	the	the	DT
cord-288669-46tkedw7	401	7	minimalistic	minimalistic	JJ
cord-288669-46tkedw7	401	8	influenza	influenza	NN
cord-288669-46tkedw7	401	9	virus	virus	NN
cord-288669-46tkedw7	401	10	M2	M2	NNP
cord-288669-46tkedw7	401	11	ion	ion	NN
cord-288669-46tkedw7	401	12	channel	channel	NN
cord-288669-46tkedw7	401	13	is	be	VBZ
cord-288669-46tkedw7	401	14	a	a	DT
cord-288669-46tkedw7	401	15	tetramer	tetramer	NN
cord-288669-46tkedw7	401	16	Molecular	Molecular	NNP
cord-288669-46tkedw7	401	17	Cloning	cloning	NN
cord-288669-46tkedw7	401	18	:	:	:
cord-288669-46tkedw7	401	19	A	a	DT
cord-288669-46tkedw7	401	20	Laboratory	Laboratory	NNP
cord-288669-46tkedw7	401	21	Manual	Manual	NNP
cord-288669-46tkedw7	402	1	How	how	WRB
cord-288669-46tkedw7	402	2	viruses	virus	NNS
cord-288669-46tkedw7	402	3	enter	enter	VBP
cord-288669-46tkedw7	402	4	animal	animal	NN
cord-288669-46tkedw7	402	5	cells	cell	NNS
cord-288669-46tkedw7	403	1	The	the	DT
cord-288669-46tkedw7	403	2	molecular	molecular	JJ
cord-288669-46tkedw7	403	3	biology	biology	NN
cord-288669-46tkedw7	403	4	of	of	IN
cord-288669-46tkedw7	403	5	arteriviruses	arteriviruse	NNS
cord-288669-46tkedw7	403	6	Identification	Identification	NNP
cord-288669-46tkedw7	403	7	of	of	IN
cord-288669-46tkedw7	403	8	a	a	DT
cord-288669-46tkedw7	403	9	novel	novel	JJ
cord-288669-46tkedw7	403	10	structural	structural	JJ
cord-288669-46tkedw7	403	11	protein	protein	NN
cord-288669-46tkedw7	403	12	of	of	IN
cord-288669-46tkedw7	403	13	arteriviruses	arteriviruses	NNP
cord-288669-46tkedw7	403	14	Topley	Topley	NNP
cord-288669-46tkedw7	403	15	and	and	CC
cord-288669-46tkedw7	403	16	Wilson	Wilson	NNP
cord-288669-46tkedw7	403	17	's	's	POS
cord-288669-46tkedw7	403	18	Microbiology	Microbiology	NNP
cord-288669-46tkedw7	403	19	and	and	CC
cord-288669-46tkedw7	403	20	Microbial	Microbial	NNP
cord-288669-46tkedw7	403	21	Infections	Infections	NNPS
cord-288669-46tkedw7	403	22	:	:	:
cord-288669-46tkedw7	404	1	Virology	Virology	NNP
cord-288669-46tkedw7	404	2	Volume	Volume	NNP
cord-288669-46tkedw7	405	1	The	the	DT
cord-288669-46tkedw7	405	2	transmembrane	transmembrane	NN
cord-288669-46tkedw7	405	3	oligomers	oligomer	NNS
cord-288669-46tkedw7	405	4	of	of	IN
cord-288669-46tkedw7	405	5	coronavirus	coronavirus	NN
cord-288669-46tkedw7	405	6	protein	protein	NN
cord-288669-46tkedw7	405	7	E	e	NN
cord-288669-46tkedw7	405	8	Proteolytic	Proteolytic	NNP
cord-288669-46tkedw7	405	9	processing	processing	NN
cord-288669-46tkedw7	405	10	of	of	IN
cord-288669-46tkedw7	405	11	the	the	DT
cord-288669-46tkedw7	405	12	open	open	JJ
cord-288669-46tkedw7	405	13	reading	reading	NN
cord-288669-46tkedw7	405	14	frame	frame	NN
cord-288669-46tkedw7	405	15	1b	1b	CD
cord-288669-46tkedw7	405	16	-	-	HYPH
cord-288669-46tkedw7	405	17	encoded	encode	VBN
cord-288669-46tkedw7	405	18	part	part	NN
cord-288669-46tkedw7	405	19	of	of	IN
cord-288669-46tkedw7	405	20	arterivirus	arterivirus	NNP
cord-288669-46tkedw7	405	21	replicase	replicase	NNP
cord-288669-46tkedw7	405	22	is	be	VBZ
cord-288669-46tkedw7	405	23	mediated	mediate	VBN
cord-288669-46tkedw7	405	24	by	by	IN
cord-288669-46tkedw7	405	25	nsp4	nsp4	NNS
cord-288669-46tkedw7	405	26	serine	serine	NN
cord-288669-46tkedw7	405	27	protease	protease	NN
cord-288669-46tkedw7	405	28	and	and	CC
cord-288669-46tkedw7	405	29	is	be	VBZ
cord-288669-46tkedw7	405	30	essential	essential	JJ
cord-288669-46tkedw7	405	31	for	for	IN
cord-288669-46tkedw7	405	32	virus	virus	NN
cord-288669-46tkedw7	405	33	replication	replication	NN
cord-288669-46tkedw7	405	34	Ion	ion	NN
cord-288669-46tkedw7	405	35	channel	channel	NN
cord-288669-46tkedw7	405	36	activity	activity	NN
cord-288669-46tkedw7	405	37	of	of	IN
cord-288669-46tkedw7	405	38	influenza	influenza	NN
cord-288669-46tkedw7	405	39	A	a	DT
cord-288669-46tkedw7	405	40	virus	virus	NN
cord-288669-46tkedw7	405	41	M2	m2	NN
cord-288669-46tkedw7	405	42	protein	protein	NN
cord-288669-46tkedw7	405	43	:	:	:
cord-288669-46tkedw7	405	44	characterization	characterization	NN
cord-288669-46tkedw7	405	45	of	of	IN
cord-288669-46tkedw7	405	46	the	the	DT
cord-288669-46tkedw7	405	47	amantadine	amantadine	NNP
cord-288669-46tkedw7	405	48	block	block	NN
cord-288669-46tkedw7	405	49	Structural	structural	JJ
cord-288669-46tkedw7	405	50	protein	protein	NN
cord-288669-46tkedw7	405	51	requirements	requirement	NNS
cord-288669-46tkedw7	405	52	in	in	IN
cord-288669-46tkedw7	405	53	equine	equine	JJ
cord-288669-46tkedw7	405	54	arteritis	arteritis	NN
cord-288669-46tkedw7	405	55	virus	virus	NN
cord-288669-46tkedw7	405	56	assembly	assembly	NN
cord-288669-46tkedw7	405	57	SARS	SARS	NNP
cord-288669-46tkedw7	405	58	coronavirus	coronavirus	NN
cord-288669-46tkedw7	405	59	E	e	NN
cord-288669-46tkedw7	405	60	protein	protein	NN
cord-288669-46tkedw7	405	61	forms	form	NNS
cord-288669-46tkedw7	405	62	cation	cation	NN
cord-288669-46tkedw7	405	63	-	-	HYPH
cord-288669-46tkedw7	405	64	selective	selective	JJ
cord-288669-46tkedw7	405	65	ion	ion	NN
cord-288669-46tkedw7	405	66	channels	channel	NNS
cord-288669-46tkedw7	405	67	Envelope	envelope	NN
cord-288669-46tkedw7	405	68	protein	protein	NN
cord-288669-46tkedw7	405	69	requirements	requirement	NNS
cord-288669-46tkedw7	405	70	for	for	IN
cord-288669-46tkedw7	405	71	the	the	DT
cord-288669-46tkedw7	405	72	assembly	assembly	NN
cord-288669-46tkedw7	405	73	of	of	IN
cord-288669-46tkedw7	405	74	infectious	infectious	JJ
cord-288669-46tkedw7	405	75	virions	virion	NNS
cord-288669-46tkedw7	405	76	of	of	IN
cord-288669-46tkedw7	405	77	porcine	porcine	JJ
cord-288669-46tkedw7	405	78	reproductive	reproductive	JJ
cord-288669-46tkedw7	405	79	and	and	CC
cord-288669-46tkedw7	405	80	respiratory	respiratory	JJ
cord-288669-46tkedw7	405	81	syndrome	syndrome	NN
cord-288669-46tkedw7	405	82	virus	virus	NN
cord-288669-46tkedw7	405	83	Homo	homo	NN
cord-288669-46tkedw7	405	84	-	-	HYPH
cord-288669-46tkedw7	405	85	oligomerization	oligomerization	NN
cord-288669-46tkedw7	405	86	of	of	IN
cord-288669-46tkedw7	405	87	the	the	DT
cord-288669-46tkedw7	405	88	porcine	porcine	JJ
cord-288669-46tkedw7	405	89	reproductive	reproductive	JJ
cord-288669-46tkedw7	405	90	and	and	CC
cord-288669-46tkedw7	405	91	respiratory	respiratory	JJ
cord-288669-46tkedw7	405	92	syndrome	syndrome	NN
cord-288669-46tkedw7	405	93	virus	virus	NN
cord-288669-46tkedw7	405	94	nucleocapsid	nucleocapsid	NN
cord-288669-46tkedw7	405	95	protein	protein	NNP
cord-288669-46tkedw7	405	96	and	and	CC
cord-288669-46tkedw7	405	97	the	the	DT
cord-288669-46tkedw7	405	98	role	role	NN
cord-288669-46tkedw7	405	99	of	of	IN
cord-288669-46tkedw7	405	100	disulfide	disulfide	NN
cord-288669-46tkedw7	405	101	linkages	linkage	NNS
cord-288669-46tkedw7	405	102	Full	full	JJ
cord-288669-46tkedw7	405	103	-	-	HYPH
cord-288669-46tkedw7	405	104	length	length	NN
cord-288669-46tkedw7	405	105	sequence	sequence	NN
cord-288669-46tkedw7	405	106	of	of	IN
cord-288669-46tkedw7	405	107	a	a	DT
cord-288669-46tkedw7	405	108	Canadian	canadian	JJ
cord-288669-46tkedw7	405	109	porcine	porcine	JJ
cord-288669-46tkedw7	405	110	reproductive	reproductive	NN
cord-288669-46tkedw7	405	111	and	and	CC
cord-288669-46tkedw7	405	112	respiratory	respiratory	JJ
cord-288669-46tkedw7	405	113	syndrome	syndrome	NN
cord-288669-46tkedw7	405	114	virus	virus	NN
cord-288669-46tkedw7	405	115	(	(	-LRB-
cord-288669-46tkedw7	405	116	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	405	117	)	)	-RRB-
cord-288669-46tkedw7	405	118	isolate	isolate	VBP
cord-288669-46tkedw7	405	119	Antigenic	antigenic	JJ
cord-288669-46tkedw7	405	120	importance	importance	NN
cord-288669-46tkedw7	405	121	of	of	IN
cord-288669-46tkedw7	405	122	the	the	DT
cord-288669-46tkedw7	405	123	carboxy	carboxy	JJ
cord-288669-46tkedw7	405	124	-	-	HYPH
cord-288669-46tkedw7	405	125	terminal	terminal	NN
cord-288669-46tkedw7	405	126	beta	beta	NN
cord-288669-46tkedw7	405	127	-	-	HYPH
cord-288669-46tkedw7	405	128	strand	strand	NN
cord-288669-46tkedw7	405	129	of	of	IN
cord-288669-46tkedw7	405	130	the	the	DT
cord-288669-46tkedw7	405	131	porcine	porcine	JJ
cord-288669-46tkedw7	405	132	reproductive	reproductive	JJ
cord-288669-46tkedw7	405	133	and	and	CC
cord-288669-46tkedw7	405	134	respiratory	respiratory	JJ
cord-288669-46tkedw7	405	135	syndrome	syndrome	NN
cord-288669-46tkedw7	405	136	virus	virus	NN
cord-288669-46tkedw7	405	137	nucleocapsid	nucleocapsid	NN
cord-288669-46tkedw7	405	138	protein	protein	NNP
cord-288669-46tkedw7	406	1	A	a	DT
cord-288669-46tkedw7	406	2	10-kDa	10-kda	CD
cord-288669-46tkedw7	406	3	structural	structural	JJ
cord-288669-46tkedw7	406	4	protein	protein	NN
cord-288669-46tkedw7	406	5	of	of	IN
cord-288669-46tkedw7	406	6	porcine	porcine	JJ
cord-288669-46tkedw7	406	7	reproductive	reproductive	JJ
cord-288669-46tkedw7	406	8	and	and	CC
cord-288669-46tkedw7	406	9	respiratory	respiratory	JJ
cord-288669-46tkedw7	406	10	syndrome	syndrome	NN
cord-288669-46tkedw7	406	11	virus	virus	NN
cord-288669-46tkedw7	406	12	encoded	encode	VBN
cord-288669-46tkedw7	406	13	by	by	IN
cord-288669-46tkedw7	406	14	ORF2b	ORF2b	NNP
cord-288669-46tkedw7	406	15	The	the	DT
cord-288669-46tkedw7	406	16	2b	2b	CD
cord-288669-46tkedw7	406	17	protein	protein	NN
cord-288669-46tkedw7	406	18	as	as	IN
cord-288669-46tkedw7	406	19	a	a	DT
cord-288669-46tkedw7	406	20	minor	minor	JJ
cord-288669-46tkedw7	406	21	structural	structural	JJ
cord-288669-46tkedw7	406	22	component	component	NN
cord-288669-46tkedw7	406	23	of	of	IN
cord-288669-46tkedw7	406	24	PRRSV	PRRSV	NNP
cord-288669-46tkedw7	407	1	This	this	DT
cord-288669-46tkedw7	407	2	study	study	NN
cord-288669-46tkedw7	407	3	was	be	VBD
cord-288669-46tkedw7	407	4	supported	support	VBN
cord-288669-46tkedw7	407	5	by	by	IN
cord-288669-46tkedw7	407	6	funding	fund	VBG
cord-288669-46tkedw7	407	7	to	to	IN
cord-288669-46tkedw7	407	8	DY	DY	NNP
cord-288669-46tkedw7	407	9	by	by	IN
cord-288669-46tkedw7	407	10	NSERC	NSERC	NNP
cord-288669-46tkedw7	407	11	Canada	Canada	NNP
cord-288669-46tkedw7	407	12	,	,	,
cord-288669-46tkedw7	407	13	Ontario	Ontario	NNP
cord-288669-46tkedw7	407	14	Pork	Pork	NNP
cord-288669-46tkedw7	407	15	,	,	,
cord-288669-46tkedw7	407	16	OMAF	OMAF	NNP
cord-288669-46tkedw7	407	17	and	and	CC
cord-288669-46tkedw7	407	18	the	the	DT
cord-288669-46tkedw7	407	19	USDA	USDA	NNP
cord-288669-46tkedw7	407	20	NRI	NRI	NNP
cord-288669-46tkedw7	407	21	PRRS	PRRS	NNP
cord-288669-46tkedw7	407	22	CAP	cap	NN
cord-288669-46tkedw7	407	23	program	program	NN
cord-288669-46tkedw7	407	24	of	of	IN
cord-288669-46tkedw7	407	25	the	the	DT
cord-288669-46tkedw7	407	26	USA	USA	NNP
cord-288669-46tkedw7	407	27	.	.	.
cord-288669-46tkedw7	408	1	The	the	DT
cord-288669-46tkedw7	408	2	authors	author	NNS
cord-288669-46tkedw7	408	3	are	be	VBP
cord-288669-46tkedw7	408	4	grateful	grateful	JJ
cord-288669-46tkedw7	408	5	to	to	IN
cord-288669-46tkedw7	408	6	Pfizer	Pfizer	NNP
cord-288669-46tkedw7	408	7	Animal	Animal	NNP
cord-288669-46tkedw7	408	8	Health	Health	NNP
cord-288669-46tkedw7	408	9	USA	USA	NNP
cord-288669-46tkedw7	408	10	for	for	IN
cord-288669-46tkedw7	408	11	providing	provide	VBG
cord-288669-46tkedw7	408	12	the	the	DT
cord-288669-46tkedw7	408	13	infectious	infectious	JJ
cord-288669-46tkedw7	408	14	cDNA	cdna	NN
cord-288669-46tkedw7	408	15	clone	clone	NN
cord-288669-46tkedw7	408	16	for	for	IN
cord-288669-46tkedw7	408	17	this	this	DT
cord-288669-46tkedw7	408	18	study	study	NN
cord-288669-46tkedw7	408	19	.	.	.