id sid tid token lemma pos cord-290231-4m9lj0uq 1 1 key key JJ cord-290231-4m9lj0uq 1 2 : : : cord-290231-4m9lj0uq 1 3 cord-290231 cord-290231 NNP cord-290231-4m9lj0uq 1 4 - - HYPH cord-290231-4m9lj0uq 1 5 4m9lj0uq 4m9lj0uq CD cord-290231-4m9lj0uq 1 6 authors author NNS cord-290231-4m9lj0uq 1 7 : : : cord-290231-4m9lj0uq 1 8 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 1 9 , , , cord-290231-4m9lj0uq 1 10 Farshad Farshad NNP cord-290231-4m9lj0uq 1 11 ; ; : cord-290231-4m9lj0uq 1 12 Bolin Bolin NNP cord-290231-4m9lj0uq 1 13 , , , cord-290231-4m9lj0uq 1 14 Richard Richard NNP cord-290231-4m9lj0uq 1 15 A. a. NN cord-290231-4m9lj0uq 1 16 ; ; : cord-290231-4m9lj0uq 1 17 Roehrig Roehrig NNP cord-290231-4m9lj0uq 1 18 , , , cord-290231-4m9lj0uq 1 19 John John NNP cord-290231-4m9lj0uq 1 20 T. T. NNP cord-290231-4m9lj0uq 2 1 title title NN cord-290231-4m9lj0uq 2 2 : : : cord-290231-4m9lj0uq 3 1 The the DT cord-290231-4m9lj0uq 3 2 Murray Murray NNP cord-290231-4m9lj0uq 3 3 Valley Valley NNP cord-290231-4m9lj0uq 3 4 encephalitis encephalitis NN cord-290231-4m9lj0uq 3 5 virus virus NN cord-290231-4m9lj0uq 3 6 prM prm JJ cord-290231-4m9lj0uq 3 7 protein protein NN cord-290231-4m9lj0uq 3 8 confers confer VBZ cord-290231-4m9lj0uq 3 9 acid acid JJ cord-290231-4m9lj0uq 3 10 resistance resistance NN cord-290231-4m9lj0uq 3 11 to to IN cord-290231-4m9lj0uq 3 12 virus virus NN cord-290231-4m9lj0uq 3 13 particles particle NNS cord-290231-4m9lj0uq 3 14 and and CC cord-290231-4m9lj0uq 3 15 alters alter VBZ cord-290231-4m9lj0uq 3 16 the the DT cord-290231-4m9lj0uq 3 17 expression expression NN cord-290231-4m9lj0uq 3 18 of of IN cord-290231-4m9lj0uq 3 19 epitopes epitope NNS cord-290231-4m9lj0uq 3 20 within within IN cord-290231-4m9lj0uq 3 21 the the DT cord-290231-4m9lj0uq 3 22 R2 R2 NNP cord-290231-4m9lj0uq 3 23 domain domain NN cord-290231-4m9lj0uq 3 24 of of IN cord-290231-4m9lj0uq 3 25 E e NN cord-290231-4m9lj0uq 3 26 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 3 27 date date NN cord-290231-4m9lj0uq 3 28 : : : cord-290231-4m9lj0uq 3 29 1992 1992 CD cord-290231-4m9lj0uq 3 30 - - SYM cord-290231-4m9lj0uq 3 31 12 12 CD cord-290231-4m9lj0uq 3 32 - - SYM cord-290231-4m9lj0uq 3 33 31 31 CD cord-290231-4m9lj0uq 3 34 journal journal NN cord-290231-4m9lj0uq 3 35 : : : cord-290231-4m9lj0uq 3 36 Virology Virology NNP cord-290231-4m9lj0uq 3 37 DOI DOI NNP cord-290231-4m9lj0uq 3 38 : : : cord-290231-4m9lj0uq 3 39 10.1016/0042 10.1016/0042 CD cord-290231-4m9lj0uq 3 40 - - HYPH cord-290231-4m9lj0uq 3 41 6822(92)90267-s 6822(92)90267-s CD cord-290231-4m9lj0uq 3 42 sha sha NNP cord-290231-4m9lj0uq 3 43 : : : cord-290231-4m9lj0uq 3 44 16aa672efa22f7191a6d18e74fa774103bb16cb7 16aa672efa22f7191a6d18e74fa774103bb16cb7 CD cord-290231-4m9lj0uq 3 45 doc_id doc_id CD cord-290231-4m9lj0uq 3 46 : : : cord-290231-4m9lj0uq 3 47 290231 290231 CD cord-290231-4m9lj0uq 3 48 cord_uid cord_uid NNS cord-290231-4m9lj0uq 3 49 : : : cord-290231-4m9lj0uq 3 50 4m9lj0uq 4m9lj0uq CD cord-290231-4m9lj0uq 3 51 Abstract Abstract NNP cord-290231-4m9lj0uq 4 1 To to TO cord-290231-4m9lj0uq 4 2 study study VB cord-290231-4m9lj0uq 4 3 the the DT cord-290231-4m9lj0uq 4 4 role role NN cord-290231-4m9lj0uq 4 5 of of IN cord-290231-4m9lj0uq 4 6 the the DT cord-290231-4m9lj0uq 4 7 precursor precursor NN cord-290231-4m9lj0uq 4 8 to to IN cord-290231-4m9lj0uq 4 9 the the DT cord-290231-4m9lj0uq 4 10 membrane membrane NN cord-290231-4m9lj0uq 4 11 protein protein NN cord-290231-4m9lj0uq 4 12 ( ( -LRB- cord-290231-4m9lj0uq 4 13 prM prM NNP cord-290231-4m9lj0uq 4 14 ) ) -RRB- cord-290231-4m9lj0uq 4 15 in in IN cord-290231-4m9lj0uq 4 16 flavivirus flavivirus JJ cord-290231-4m9lj0uq 4 17 maturation maturation NN cord-290231-4m9lj0uq 4 18 , , , cord-290231-4m9lj0uq 4 19 we -PRON- PRP cord-290231-4m9lj0uq 4 20 inhibited inhibit VBD cord-290231-4m9lj0uq 4 21 the the DT cord-290231-4m9lj0uq 4 22 proteolytic proteolytic JJ cord-290231-4m9lj0uq 4 23 processing processing NN cord-290231-4m9lj0uq 4 24 of of IN cord-290231-4m9lj0uq 4 25 the the DT cord-290231-4m9lj0uq 4 26 Murray Murray NNP cord-290231-4m9lj0uq 4 27 Valley Valley NNP cord-290231-4m9lj0uq 4 28 encephalitis encephalitis RBR cord-290231-4m9lj0uq 4 29 ( ( -LRB- cord-290231-4m9lj0uq 4 30 MVE MVE NNP cord-290231-4m9lj0uq 4 31 ) ) -RRB- cord-290231-4m9lj0uq 4 32 virus virus NN cord-290231-4m9lj0uq 4 33 prM prm JJ cord-290231-4m9lj0uq 4 34 to to IN cord-290231-4m9lj0uq 4 35 membrane membrane NN cord-290231-4m9lj0uq 4 36 protein protein NN cord-290231-4m9lj0uq 4 37 in in IN cord-290231-4m9lj0uq 4 38 infected infected JJ cord-290231-4m9lj0uq 4 39 cells cell NNS cord-290231-4m9lj0uq 4 40 by by IN cord-290231-4m9lj0uq 4 41 adding add VBG cord-290231-4m9lj0uq 4 42 the the DT cord-290231-4m9lj0uq 4 43 acidotropic acidotropic NNP cord-290231-4m9lj0uq 4 44 agent agent NN cord-290231-4m9lj0uq 4 45 ammonium ammonium NN cord-290231-4m9lj0uq 4 46 chloride chloride NN cord-290231-4m9lj0uq 4 47 late late RB cord-290231-4m9lj0uq 4 48 in in IN cord-290231-4m9lj0uq 4 49 the the DT cord-290231-4m9lj0uq 4 50 virus virus NN cord-290231-4m9lj0uq 4 51 replication replication NN cord-290231-4m9lj0uq 4 52 cycle cycle NN cord-290231-4m9lj0uq 4 53 . . . cord-290231-4m9lj0uq 5 1 Viruses virus NNS cord-290231-4m9lj0uq 5 2 purified purify VBN cord-290231-4m9lj0uq 5 3 from from IN cord-290231-4m9lj0uq 5 4 supernatants supernatant NNS cord-290231-4m9lj0uq 5 5 of of IN cord-290231-4m9lj0uq 5 6 ammonium ammonium NN cord-290231-4m9lj0uq 5 7 chloride chloride NN cord-290231-4m9lj0uq 5 8 - - HYPH cord-290231-4m9lj0uq 5 9 treated treat VBN cord-290231-4m9lj0uq 5 10 cells cell NNS cord-290231-4m9lj0uq 5 11 contained contain VBD cord-290231-4m9lj0uq 5 12 prM prm JJ cord-290231-4m9lj0uq 5 13 protein protein NN cord-290231-4m9lj0uq 5 14 and and CC cord-290231-4m9lj0uq 5 15 were be VBD cord-290231-4m9lj0uq 5 16 unable unable JJ cord-290231-4m9lj0uq 5 17 to to TO cord-290231-4m9lj0uq 5 18 fuse fuse VB cord-290231-4m9lj0uq 5 19 C6/36 C6/36 NNP cord-290231-4m9lj0uq 5 20 mosquito mosquito NN cord-290231-4m9lj0uq 5 21 cells cell NNS cord-290231-4m9lj0uq 5 22 from from IN cord-290231-4m9lj0uq 5 23 without without IN cord-290231-4m9lj0uq 5 24 . . . cord-290231-4m9lj0uq 6 1 When when WRB cord-290231-4m9lj0uq 6 2 ammonium ammonium NN cord-290231-4m9lj0uq 6 3 chloride chloride NN cord-290231-4m9lj0uq 6 4 was be VBD cord-290231-4m9lj0uq 6 5 removed remove VBN cord-290231-4m9lj0uq 6 6 from from IN cord-290231-4m9lj0uq 6 7 the the DT cord-290231-4m9lj0uq 6 8 cells cell NNS cord-290231-4m9lj0uq 6 9 , , , cord-290231-4m9lj0uq 6 10 both both CC cord-290231-4m9lj0uq 6 11 the the DT cord-290231-4m9lj0uq 6 12 processing processing NN cord-290231-4m9lj0uq 6 13 of of IN cord-290231-4m9lj0uq 6 14 prM prm JJ cord-290231-4m9lj0uq 6 15 and and CC cord-290231-4m9lj0uq 6 16 the the DT cord-290231-4m9lj0uq 6 17 fusion fusion NN cord-290231-4m9lj0uq 6 18 activity activity NN cord-290231-4m9lj0uq 6 19 of of IN cord-290231-4m9lj0uq 6 20 the the DT cord-290231-4m9lj0uq 6 21 purified purify VBN cord-290231-4m9lj0uq 6 22 viruses virus NNS cord-290231-4m9lj0uq 6 23 were be VBD cord-290231-4m9lj0uq 6 24 partially partially RB cord-290231-4m9lj0uq 6 25 restored restore VBN cord-290231-4m9lj0uq 6 26 . . . cord-290231-4m9lj0uq 7 1 By by IN cord-290231-4m9lj0uq 7 2 using use VBG cord-290231-4m9lj0uq 7 3 monoclonal monoclonal JJ cord-290231-4m9lj0uq 7 4 antibodies antibody NNS cord-290231-4m9lj0uq 7 5 ( ( -LRB- cord-290231-4m9lj0uq 7 6 MAbs MAbs NNP cord-290231-4m9lj0uq 7 7 ) ) -RRB- cord-290231-4m9lj0uq 7 8 specific specific JJ cord-290231-4m9lj0uq 7 9 for for IN cord-290231-4m9lj0uq 7 10 the the DT cord-290231-4m9lj0uq 7 11 envelope envelope NN cord-290231-4m9lj0uq 7 12 ( ( -LRB- cord-290231-4m9lj0uq 7 13 E e NN cord-290231-4m9lj0uq 7 14 ) ) -RRB- cord-290231-4m9lj0uq 7 15 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 7 16 of of IN cord-290231-4m9lj0uq 7 17 MVE MVE NNP cord-290231-4m9lj0uq 7 18 virus virus NN cord-290231-4m9lj0uq 7 19 , , , cord-290231-4m9lj0uq 7 20 we -PRON- PRP cord-290231-4m9lj0uq 7 21 found find VBD cord-290231-4m9lj0uq 7 22 that that IN cord-290231-4m9lj0uq 7 23 at at RB cord-290231-4m9lj0uq 7 24 least least RBS cord-290231-4m9lj0uq 7 25 three three CD cord-290231-4m9lj0uq 7 26 epitopes epitope NNS cord-290231-4m9lj0uq 7 27 were be VBD cord-290231-4m9lj0uq 7 28 less less RBR cord-290231-4m9lj0uq 7 29 accessible accessible JJ cord-290231-4m9lj0uq 7 30 to to IN cord-290231-4m9lj0uq 7 31 their -PRON- PRP$ cord-290231-4m9lj0uq 7 32 corresponding correspond VBG cord-290231-4m9lj0uq 7 33 antibodies antibody NNS cord-290231-4m9lj0uq 7 34 in in IN cord-290231-4m9lj0uq 7 35 the the DT cord-290231-4m9lj0uq 7 36 prM prm NN cord-290231-4m9lj0uq 7 37 - - HYPH cord-290231-4m9lj0uq 7 38 containing contain VBG cord-290231-4m9lj0uq 7 39 MVE MVE NNP cord-290231-4m9lj0uq 7 40 virus virus NN cord-290231-4m9lj0uq 7 41 particles particle NNS cord-290231-4m9lj0uq 7 42 . . . cord-290231-4m9lj0uq 8 1 Amino amino NN cord-290231-4m9lj0uq 8 2 - - HYPH cord-290231-4m9lj0uq 8 3 terminal terminal NN cord-290231-4m9lj0uq 8 4 sequencing sequencing NN cord-290231-4m9lj0uq 8 5 of of IN cord-290231-4m9lj0uq 8 6 proteolytic proteolytic JJ cord-290231-4m9lj0uq 8 7 fragments fragment NNS cord-290231-4m9lj0uq 8 8 of of IN cord-290231-4m9lj0uq 8 9 the the DT cord-290231-4m9lj0uq 8 10 E e NN cord-290231-4m9lj0uq 8 11 protein protein NN cord-290231-4m9lj0uq 8 12 which which WDT cord-290231-4m9lj0uq 8 13 were be VBD cord-290231-4m9lj0uq 8 14 reactive reactive JJ cord-290231-4m9lj0uq 8 15 with with IN cord-290231-4m9lj0uq 8 16 sequence sequence NN cord-290231-4m9lj0uq 8 17 - - HYPH cord-290231-4m9lj0uq 8 18 specific specific JJ cord-290231-4m9lj0uq 8 19 peptide peptide NN cord-290231-4m9lj0uq 8 20 antisera antisera NN cord-290231-4m9lj0uq 8 21 or or CC cord-290231-4m9lj0uq 8 22 MAb MAb NNS cord-290231-4m9lj0uq 8 23 enabled enable VBD cord-290231-4m9lj0uq 8 24 us -PRON- PRP cord-290231-4m9lj0uq 8 25 to to TO cord-290231-4m9lj0uq 8 26 estimate estimate VB cord-290231-4m9lj0uq 8 27 the the DT cord-290231-4m9lj0uq 8 28 site site NN cord-290231-4m9lj0uq 8 29 of of IN cord-290231-4m9lj0uq 8 30 the the DT cord-290231-4m9lj0uq 8 31 E e NN cord-290231-4m9lj0uq 8 32 protein protein NN cord-290231-4m9lj0uq 8 33 interacting interact VBG cord-290231-4m9lj0uq 8 34 with with IN cord-290231-4m9lj0uq 8 35 the the DT cord-290231-4m9lj0uq 8 36 prM prm NN cord-290231-4m9lj0uq 8 37 to to TO cord-290231-4m9lj0uq 8 38 be be VB cord-290231-4m9lj0uq 8 39 within within IN cord-290231-4m9lj0uq 8 40 amino amino NN cord-290231-4m9lj0uq 8 41 acids acid NNS cord-290231-4m9lj0uq 8 42 200 200 CD cord-290231-4m9lj0uq 8 43 to to IN cord-290231-4m9lj0uq 8 44 327 327 CD cord-290231-4m9lj0uq 8 45 . . . cord-290231-4m9lj0uq 9 1 Since since IN cord-290231-4m9lj0uq 9 2 prM prm NN cord-290231-4m9lj0uq 9 3 - - HYPH cord-290231-4m9lj0uq 9 4 containing contain VBG cord-290231-4m9lj0uq 9 5 viruses virus NNS cord-290231-4m9lj0uq 9 6 were be VBD cord-290231-4m9lj0uq 9 7 up up RB cord-290231-4m9lj0uq 9 8 to to IN cord-290231-4m9lj0uq 9 9 400-fold 400-fold CD cord-290231-4m9lj0uq 9 10 more more RBR cord-290231-4m9lj0uq 9 11 resistant resistant JJ cord-290231-4m9lj0uq 9 12 to to IN cord-290231-4m9lj0uq 9 13 a a DT cord-290231-4m9lj0uq 9 14 low low JJ cord-290231-4m9lj0uq 9 15 pH pH NNP cord-290231-4m9lj0uq 9 16 environment environment NN cord-290231-4m9lj0uq 9 17 , , , cord-290231-4m9lj0uq 9 18 we -PRON- PRP cord-290231-4m9lj0uq 9 19 conclude conclude VBP cord-290231-4m9lj0uq 9 20 that that IN cord-290231-4m9lj0uq 9 21 the the DT cord-290231-4m9lj0uq 9 22 E e NN cord-290231-4m9lj0uq 9 23 - - JJ cord-290231-4m9lj0uq 9 24 prM prm JJ cord-290231-4m9lj0uq 9 25 interaction interaction NN cord-290231-4m9lj0uq 9 26 might may MD cord-290231-4m9lj0uq 9 27 be be VB cord-290231-4m9lj0uq 9 28 necessary necessary JJ cord-290231-4m9lj0uq 9 29 to to TO cord-290231-4m9lj0uq 9 30 protect protect VB cord-290231-4m9lj0uq 9 31 the the DT cord-290231-4m9lj0uq 9 32 E e NN cord-290231-4m9lj0uq 9 33 protein protein NN cord-290231-4m9lj0uq 9 34 from from IN cord-290231-4m9lj0uq 9 35 irreversible irreversible JJ cord-290231-4m9lj0uq 9 36 conformational conformational JJ cord-290231-4m9lj0uq 9 37 changes change NNS cord-290231-4m9lj0uq 9 38 caused cause VBN cord-290231-4m9lj0uq 9 39 by by IN cord-290231-4m9lj0uq 9 40 maturation maturation NN cord-290231-4m9lj0uq 9 41 into into IN cord-290231-4m9lj0uq 9 42 the the DT cord-290231-4m9lj0uq 9 43 acidic acidic JJ cord-290231-4m9lj0uq 9 44 vesicles vesicle NNS cord-290231-4m9lj0uq 9 45 of of IN cord-290231-4m9lj0uq 9 46 the the DT cord-290231-4m9lj0uq 9 47 exocytic exocytic JJ cord-290231-4m9lj0uq 9 48 pathway pathway NN cord-290231-4m9lj0uq 9 49 . . . cord-290231-4m9lj0uq 10 1 For for IN cord-290231-4m9lj0uq 10 2 enveloped envelop VBN cord-290231-4m9lj0uq 10 3 viruses virus NNS cord-290231-4m9lj0uq 10 4 to to TO cord-290231-4m9lj0uq 10 5 enter enter VB cord-290231-4m9lj0uq 10 6 into into IN cord-290231-4m9lj0uq 10 7 their -PRON- PRP$ cord-290231-4m9lj0uq 10 8 target target NN cord-290231-4m9lj0uq 10 9 cells cell NNS cord-290231-4m9lj0uq 10 10 , , , cord-290231-4m9lj0uq 10 11 virus virus NN cord-290231-4m9lj0uq 10 12 - - HYPH cord-290231-4m9lj0uq 10 13 cell cell NN cord-290231-4m9lj0uq 10 14 membrane membrane NN cord-290231-4m9lj0uq 10 15 fusion fusion NN cord-290231-4m9lj0uq 10 16 must must MD cord-290231-4m9lj0uq 10 17 take take VB cord-290231-4m9lj0uq 10 18 place place NN cord-290231-4m9lj0uq 10 19 to to TO cord-290231-4m9lj0uq 10 20 release release VB cord-290231-4m9lj0uq 10 21 the the DT cord-290231-4m9lj0uq 10 22 nucleocapsid nucleocapsid NN cord-290231-4m9lj0uq 10 23 into into IN cord-290231-4m9lj0uq 10 24 the the DT cord-290231-4m9lj0uq 10 25 cytoplasm cytoplasm NN cord-290231-4m9lj0uq 10 26 . . . cord-290231-4m9lj0uq 11 1 This this DT cord-290231-4m9lj0uq 11 2 occurs occur VBZ cord-290231-4m9lj0uq 11 3 either either CC cord-290231-4m9lj0uq 11 4 directly directly RB cord-290231-4m9lj0uq 11 5 at at IN cord-290231-4m9lj0uq 11 6 the the DT cord-290231-4m9lj0uq 11 7 plasma plasma NN cord-290231-4m9lj0uq 11 8 membrane membrane NN cord-290231-4m9lj0uq 11 9 , , , cord-290231-4m9lj0uq 11 10 as as IN cord-290231-4m9lj0uq 11 11 with with IN cord-290231-4m9lj0uq 11 12 paramyxoviruses paramyxovirus NNS cord-290231-4m9lj0uq 11 13 and and CC cord-290231-4m9lj0uq 11 14 herpesviruses herpesviruses NNP cord-290231-4m9lj0uq 11 15 , , , cord-290231-4m9lj0uq 11 16 or or CC cord-290231-4m9lj0uq 11 17 in in IN cord-290231-4m9lj0uq 11 18 the the DT cord-290231-4m9lj0uq 11 19 endosomal endosomal NNP cord-290231-4m9lj0uq 11 20 vesicles vesicle NNS cord-290231-4m9lj0uq 11 21 after after IN cord-290231-4m9lj0uq 11 22 the the DT cord-290231-4m9lj0uq 11 23 viruses virus NNS cord-290231-4m9lj0uq 11 24 are be VBP cord-290231-4m9lj0uq 11 25 taken take VBN cord-290231-4m9lj0uq 11 26 up up RP cord-290231-4m9lj0uq 11 27 by by IN cord-290231-4m9lj0uq 11 28 receptor receptor NN cord-290231-4m9lj0uq 11 29 - - HYPH cord-290231-4m9lj0uq 11 30 mediated mediate VBN cord-290231-4m9lj0uq 11 31 endocytosis endocytosis NN cord-290231-4m9lj0uq 11 32 , , , cord-290231-4m9lj0uq 11 33 as as IN cord-290231-4m9lj0uq 11 34 with with IN cord-290231-4m9lj0uq 11 35 togaviruses togaviruses NNP cord-290231-4m9lj0uq 11 36 and and CC cord-290231-4m9lj0uq 11 37 orthomyxoviruses orthomyxoviruses NNP cord-290231-4m9lj0uq 11 38 ( ( -LRB- cord-290231-4m9lj0uq 11 39 for for IN cord-290231-4m9lj0uq 11 40 review review NN cord-290231-4m9lj0uq 11 41 see see VB cord-290231-4m9lj0uq 11 42 Marsh Marsh NNP cord-290231-4m9lj0uq 11 43 and and CC cord-290231-4m9lj0uq 11 44 Helenius Helenius NNP cord-290231-4m9lj0uq 11 45 , , , cord-290231-4m9lj0uq 11 46 1989 1989 CD cord-290231-4m9lj0uq 11 47 ) ) -RRB- cord-290231-4m9lj0uq 11 48 . . . cord-290231-4m9lj0uq 12 1 Generally generally RB cord-290231-4m9lj0uq 12 2 the the DT cord-290231-4m9lj0uq 12 3 fusion fusion NN cord-290231-4m9lj0uq 12 4 - - HYPH cord-290231-4m9lj0uq 12 5 mediating mediate VBG cord-290231-4m9lj0uq 12 6 envelope envelope NN cord-290231-4m9lj0uq 12 7 proteins protein NNS cord-290231-4m9lj0uq 12 8 are be VBP cord-290231-4m9lj0uq 12 9 activated activate VBN cord-290231-4m9lj0uq 12 10 by by IN cord-290231-4m9lj0uq 12 11 proteolytic proteolytic JJ cord-290231-4m9lj0uq 12 12 cleavage cleavage NN cord-290231-4m9lj0uq 12 13 of of IN cord-290231-4m9lj0uq 12 14 the the DT cord-290231-4m9lj0uq 12 15 spike spike NN cord-290231-4m9lj0uq 12 16 precursor precursor NN cord-290231-4m9lj0uq 12 17 proteins protein NNS cord-290231-4m9lj0uq 12 18 by by IN cord-290231-4m9lj0uq 12 19 a a DT cord-290231-4m9lj0uq 12 20 host host NN cord-290231-4m9lj0uq 12 21 protease protease NN cord-290231-4m9lj0uq 12 22 late late RB cord-290231-4m9lj0uq 12 23 during during IN cord-290231-4m9lj0uq 12 24 virus virus NN cord-290231-4m9lj0uq 12 25 assembly assembly NN cord-290231-4m9lj0uq 12 26 . . . cord-290231-4m9lj0uq 13 1 Cleavage cleavage NN cord-290231-4m9lj0uq 13 2 activation activation NN cord-290231-4m9lj0uq 13 3 of of IN cord-290231-4m9lj0uq 13 4 the the DT cord-290231-4m9lj0uq 13 5 glycoproteins glycoprotein NNS cord-290231-4m9lj0uq 13 6 involved involve VBN cord-290231-4m9lj0uq 13 7 in in IN cord-290231-4m9lj0uq 13 8 fusion fusion NN cord-290231-4m9lj0uq 13 9 has have VBZ cord-290231-4m9lj0uq 13 10 been be VBN cord-290231-4m9lj0uq 13 11 demonstrated demonstrate VBN cord-290231-4m9lj0uq 13 12 with with IN cord-290231-4m9lj0uq 13 13 the the DT cord-290231-4m9lj0uq 13 14 gPE2 gpe2 NN cord-290231-4m9lj0uq 13 15 of of IN cord-290231-4m9lj0uq 13 16 murine murine JJ cord-290231-4m9lj0uq 13 17 coronaviruses coronaviruse NNS cord-290231-4m9lj0uq 13 18 ( ( -LRB- cord-290231-4m9lj0uq 13 19 Sturman Sturman NNP cord-290231-4m9lj0uq 13 20 and and CC cord-290231-4m9lj0uq 13 21 Holmes Holmes NNP cord-290231-4m9lj0uq 13 22 , , , cord-290231-4m9lj0uq 13 23 1984 1984 CD cord-290231-4m9lj0uq 13 24 ; ; : cord-290231-4m9lj0uq 13 25 Sturman Sturman NNP cord-290231-4m9lj0uq 13 26 et et NNP cord-290231-4m9lj0uq 13 27 al al NNP cord-290231-4m9lj0uq 13 28 . . . cord-290231-4m9lj0uq 14 1 , , , cord-290231-4m9lj0uq 14 2 1985 1985 CD cord-290231-4m9lj0uq 14 3 ) ) -RRB- cord-290231-4m9lj0uq 14 4 the the DT cord-290231-4m9lj0uq 14 5 HA HA NNP cord-290231-4m9lj0uq 14 6 of of IN cord-290231-4m9lj0uq 14 7 influenza influenza NN cord-290231-4m9lj0uq 14 8 viruses virus NNS cord-290231-4m9lj0uq 14 9 ( ( -LRB- cord-290231-4m9lj0uq 14 10 Wiley Wiley NNP cord-290231-4m9lj0uq 14 11 and and CC cord-290231-4m9lj0uq 14 12 Skehel Skehel NNP cord-290231-4m9lj0uq 14 13 , , , cord-290231-4m9lj0uq 14 14 1987 1987 CD cord-290231-4m9lj0uq 14 15 ) ) -RRB- cord-290231-4m9lj0uq 14 16 the the DT cord-290231-4m9lj0uq 14 17 gP160 gp160 CD cord-290231-4m9lj0uq 14 18 of of IN cord-290231-4m9lj0uq 14 19 retroviruses retrovirus NNS cord-290231-4m9lj0uq 14 20 ( ( -LRB- cord-290231-4m9lj0uq 14 21 McCune McCune NNP cord-290231-4m9lj0uq 14 22 et et FW cord-290231-4m9lj0uq 14 23 al al NNP cord-290231-4m9lj0uq 14 24 . . . cord-290231-4m9lj0uq 15 1 , , , cord-290231-4m9lj0uq 15 2 1988 1988 CD cord-290231-4m9lj0uq 15 3 ; ; : cord-290231-4m9lj0uq 15 4 Willey Willey NNP cord-290231-4m9lj0uq 15 5 et et NNP cord-290231-4m9lj0uq 15 6 al al NNP cord-290231-4m9lj0uq 15 7 . . NNP cord-290231-4m9lj0uq 15 8 , , , cord-290231-4m9lj0uq 15 9 1991 1991 CD cord-290231-4m9lj0uq 15 10 ) ) -RRB- cord-290231-4m9lj0uq 15 11 and and CC cord-290231-4m9lj0uq 15 12 the the DT cord-290231-4m9lj0uq 15 13 PE2 PE2 NNP cord-290231-4m9lj0uq 15 14 of of IN cord-290231-4m9lj0uq 15 15 alphaviruses alphaviruses NNP cord-290231-4m9lj0uq 16 1 ( ( -LRB- cord-290231-4m9lj0uq 16 2 Lobigs Lobigs NNP cord-290231-4m9lj0uq 16 3 and and CC cord-290231-4m9lj0uq 16 4 Garoff Garoff NNP cord-290231-4m9lj0uq 16 5 , , , cord-290231-4m9lj0uq 16 6 1990 1990 CD cord-290231-4m9lj0uq 16 7 ; ; : cord-290231-4m9lj0uq 16 8 Lobigs Lobigs NNP cord-290231-4m9lj0uq 16 9 et et NNP cord-290231-4m9lj0uq 16 10 a/. a/. NNP cord-290231-4m9lj0uq 16 11 , , , cord-290231-4m9lj0uq 16 12 1990 1990 CD cord-290231-4m9lj0uq 16 13 ; ; : cord-290231-4m9lj0uq 17 1 White White NNP cord-290231-4m9lj0uq 17 2 et et NNP cord-290231-4m9lj0uq 17 3 al al NNP cord-290231-4m9lj0uq 17 4 . . NNP cord-290231-4m9lj0uq 17 5 , , , cord-290231-4m9lj0uq 17 6 1983 1983 CD cord-290231-4m9lj0uq 17 7 ) ) -RRB- cord-290231-4m9lj0uq 17 8 . . . cord-290231-4m9lj0uq 18 1 The the DT cord-290231-4m9lj0uq 18 2 cleaved cleaved JJ cord-290231-4m9lj0uq 18 3 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 18 4 ( ( -LRB- cord-290231-4m9lj0uq 18 5 e.g. e.g. RB cord-290231-4m9lj0uq 18 6 , , , cord-290231-4m9lj0uq 18 7 HA HA NNP cord-290231-4m9lj0uq 18 8 ) ) -RRB- cord-290231-4m9lj0uq 18 9 undergoes undergo VBZ cord-290231-4m9lj0uq 18 10 an an DT cord-290231-4m9lj0uq 18 11 irreversible irreversible JJ cord-290231-4m9lj0uq 18 12 conformational conformational JJ cord-290231-4m9lj0uq 18 13 change change NN cord-290231-4m9lj0uq 18 14 in in IN cord-290231-4m9lj0uq 18 15 the the DT cord-290231-4m9lj0uq 18 16 acidic acidic JJ cord-290231-4m9lj0uq 18 17 pH pH NNP cord-290231-4m9lj0uq 18 18 milieu milieu NN cord-290231-4m9lj0uq 18 19 of of IN cord-290231-4m9lj0uq 18 20 the the DT cord-290231-4m9lj0uq 18 21 endocytic endocytic JJ cord-290231-4m9lj0uq 18 22 vesicles vesicle NNS cord-290231-4m9lj0uq 18 23 which which WDT cord-290231-4m9lj0uq 18 24 exposes expose VBZ cord-290231-4m9lj0uq 18 25 a a DT cord-290231-4m9lj0uq 18 26 hydrophobic hydrophobic JJ cord-290231-4m9lj0uq 18 27 fusion fusion NN cord-290231-4m9lj0uq 18 28 site site NN cord-290231-4m9lj0uq 18 29 that that WDT cord-290231-4m9lj0uq 18 30 mediates mediate VBZ cord-290231-4m9lj0uq 18 31 the the DT cord-290231-4m9lj0uq 18 32 virus virus NN cord-290231-4m9lj0uq 18 33 - - HYPH cord-290231-4m9lj0uq 18 34 cell cell NN cord-290231-4m9lj0uq 18 35 membrane membrane NN cord-290231-4m9lj0uq 18 36 fusion fusion NN cord-290231-4m9lj0uq 18 37 . . . cord-290231-4m9lj0uq 19 1 This this DT cord-290231-4m9lj0uq 19 2 cleavage cleavage NN cord-290231-4m9lj0uq 19 3 of of IN cord-290231-4m9lj0uq 19 4 the the DT cord-290231-4m9lj0uq 19 5 precursor precursor NN cord-290231-4m9lj0uq 19 6 protein protein NN cord-290231-4m9lj0uq 19 7 is be VBZ cord-290231-4m9lj0uq 19 8 essential essential JJ cord-290231-4m9lj0uq 19 9 for for IN cord-290231-4m9lj0uq 19 10 virus virus NN cord-290231-4m9lj0uq 19 11 infectivity infectivity NN cord-290231-4m9lj0uq 19 12 but but CC cord-290231-4m9lj0uq 19 13 not not RB cord-290231-4m9lj0uq 19 14 particle particle NN cord-290231-4m9lj0uq 19 15 formation formation NN cord-290231-4m9lj0uq 19 16 . . . cord-290231-4m9lj0uq 20 1 A a DT cord-290231-4m9lj0uq 20 2 Semliki Semliki NNP cord-290231-4m9lj0uq 20 3 Forest Forest NNP cord-290231-4m9lj0uq 20 4 virus virus NN cord-290231-4m9lj0uq 21 1 ( ( -LRB- cord-290231-4m9lj0uq 21 2 SFV SFV NNP cord-290231-4m9lj0uq 21 3 ) ) -RRB- cord-290231-4m9lj0uq 21 4 mutant mutant NN cord-290231-4m9lj0uq 21 5 defective defective JJ cord-290231-4m9lj0uq 21 6 in in IN cord-290231-4m9lj0uq 21 7 PE2 PE2 NNP cord-290231-4m9lj0uq 21 8 cleavage cleavage NN cord-290231-4m9lj0uq 21 9 expressed express VBD cord-290231-4m9lj0uq 21 10 PE2 PE2 NNP cord-290231-4m9lj0uq 21 11 at at IN cord-290231-4m9lj0uq 21 12 the the DT cord-290231-4m9lj0uq 21 13 cell cell NN cord-290231-4m9lj0uq 21 14 ' ' '' cord-290231-4m9lj0uq 21 15 To to TO cord-290231-4m9lj0uq 21 16 whom whom WP cord-290231-4m9lj0uq 21 17 reprint reprint VB cord-290231-4m9lj0uq 21 18 requests request NNS cord-290231-4m9lj0uq 21 19 should should MD cord-290231-4m9lj0uq 21 20 be be VB cord-290231-4m9lj0uq 21 21 addressed address VBN cord-290231-4m9lj0uq 21 22 . . . cord-290231-4m9lj0uq 22 1 surface surface NN cord-290231-4m9lj0uq 22 2 but but CC cord-290231-4m9lj0uq 22 3 did do VBD cord-290231-4m9lj0uq 22 4 not not RB cord-290231-4m9lj0uq 22 5 function function VB cord-290231-4m9lj0uq 22 6 in in IN cord-290231-4m9lj0uq 22 7 low low JJ cord-290231-4m9lj0uq 22 8 pH ph NN cord-290231-4m9lj0uq 22 9 - - HYPH cord-290231-4m9lj0uq 22 10 triggered trigger VBN cord-290231-4m9lj0uq 22 11 cell cell NN cord-290231-4m9lj0uq 22 12 - - HYPH cord-290231-4m9lj0uq 22 13 tocell tocell NN cord-290231-4m9lj0uq 22 14 fusion fusion NN cord-290231-4m9lj0uq 22 15 ( ( -LRB- cord-290231-4m9lj0uq 22 16 Lobigs Lobigs NNP cord-290231-4m9lj0uq 22 17 and and CC cord-290231-4m9lj0uq 22 18 Garoff Garoff NNP cord-290231-4m9lj0uq 22 19 , , , cord-290231-4m9lj0uq 22 20 1990 1990 CD cord-290231-4m9lj0uq 22 21 ; ; : cord-290231-4m9lj0uq 22 22 Lobigs Lobigs NNP cord-290231-4m9lj0uq 22 23 et et NNP cord-290231-4m9lj0uq 22 24 al al NNP cord-290231-4m9lj0uq 22 25 . . NNP cord-290231-4m9lj0uq 22 26 , , , cord-290231-4m9lj0uq 22 27 1990 1990 CD cord-290231-4m9lj0uq 22 28 ) ) -RRB- cord-290231-4m9lj0uq 22 29 . . . cord-290231-4m9lj0uq 23 1 Murray Murray NNP cord-290231-4m9lj0uq 23 2 Valley Valley NNP cord-290231-4m9lj0uq 23 3 encephalitis encephalitis RB cord-290231-4m9lj0uq 23 4 ( ( -LRB- cord-290231-4m9lj0uq 23 5 MVE MVE NNP cord-290231-4m9lj0uq 23 6 ) ) -RRB- cord-290231-4m9lj0uq 23 7 virus virus NN cord-290231-4m9lj0uq 23 8 is be VBZ cord-290231-4m9lj0uq 23 9 a a DT cord-290231-4m9lj0uq 23 10 member member NN cord-290231-4m9lj0uq 23 11 of of IN cord-290231-4m9lj0uq 23 12 the the DT cord-290231-4m9lj0uq 23 13 Flaviviridae Flaviviridae NNP cord-290231-4m9lj0uq 23 14 , , , cord-290231-4m9lj0uq 23 15 a a DT cord-290231-4m9lj0uq 23 16 family family NN cord-290231-4m9lj0uq 23 17 of of IN cord-290231-4m9lj0uq 23 18 small small JJ cord-290231-4m9lj0uq 23 19 , , , cord-290231-4m9lj0uq 23 20 positive positive JJ cord-290231-4m9lj0uq 23 21 - - HYPH cord-290231-4m9lj0uq 23 22 strand strand NN cord-290231-4m9lj0uq 23 23 RNA RNA NNP cord-290231-4m9lj0uq 23 24 viruses virus NNS cord-290231-4m9lj0uq 23 25 . . . cord-290231-4m9lj0uq 24 1 The the DT cord-290231-4m9lj0uq 24 2 mature mature JJ cord-290231-4m9lj0uq 24 3 flavivirions flavivirion NNS cord-290231-4m9lj0uq 24 4 contain contain VBP cord-290231-4m9lj0uq 24 5 three three CD cord-290231-4m9lj0uq 24 6 structural structural JJ cord-290231-4m9lj0uq 24 7 proteins protein NNS cord-290231-4m9lj0uq 24 8 : : : cord-290231-4m9lj0uq 24 9 capsid capsid NN cord-290231-4m9lj0uq 24 10 ( ( -LRB- cord-290231-4m9lj0uq 24 11 C C NNP cord-290231-4m9lj0uq 24 12 ) ) -RRB- cord-290231-4m9lj0uq 24 13 , , , cord-290231-4m9lj0uq 24 14 membrane membrane NN cord-290231-4m9lj0uq 24 15 ( ( -LRB- cord-290231-4m9lj0uq 24 16 M M NNP cord-290231-4m9lj0uq 24 17 ) ) -RRB- cord-290231-4m9lj0uq 24 18 , , , cord-290231-4m9lj0uq 24 19 and and CC cord-290231-4m9lj0uq 24 20 envelope envelope NN cord-290231-4m9lj0uq 24 21 ( ( -LRB- cord-290231-4m9lj0uq 24 22 E E NNP cord-290231-4m9lj0uq 24 23 ) ) -RRB- cord-290231-4m9lj0uq 24 24 , , , cord-290231-4m9lj0uq 24 25 with with IN cord-290231-4m9lj0uq 24 26 molecular molecular JJ cord-290231-4m9lj0uq 24 27 masses masse NNS cord-290231-4m9lj0uq 24 28 of of IN cord-290231-4m9lj0uq 24 29 14 14 CD cord-290231-4m9lj0uq 24 30 - - SYM cord-290231-4m9lj0uq 24 31 15 15 CD cord-290231-4m9lj0uq 24 32 , , , cord-290231-4m9lj0uq 24 33 7 7 CD cord-290231-4m9lj0uq 24 34 - - SYM cord-290231-4m9lj0uq 24 35 8 8 CD cord-290231-4m9lj0uq 24 36 , , , cord-290231-4m9lj0uq 24 37 and and CC cord-290231-4m9lj0uq 24 38 50 50 CD cord-290231-4m9lj0uq 24 39 - - SYM cord-290231-4m9lj0uq 24 40 60 60 CD cord-290231-4m9lj0uq 24 41 kDa kDa NNS cord-290231-4m9lj0uq 24 42 , , , cord-290231-4m9lj0uq 24 43 respectively respectively RB cord-290231-4m9lj0uq 24 44 . . . cord-290231-4m9lj0uq 25 1 The the DT cord-290231-4m9lj0uq 25 2 E e NN cord-290231-4m9lj0uq 25 3 protein protein NN cord-290231-4m9lj0uq 25 4 is be VBZ cord-290231-4m9lj0uq 25 5 the the DT cord-290231-4m9lj0uq 25 6 only only JJ cord-290231-4m9lj0uq 25 7 structural structural JJ cord-290231-4m9lj0uq 25 8 protein protein NN cord-290231-4m9lj0uq 25 9 responsible responsible JJ cord-290231-4m9lj0uq 25 10 for for IN cord-290231-4m9lj0uq 25 11 the the DT cord-290231-4m9lj0uq 25 12 induction induction NN cord-290231-4m9lj0uq 25 13 of of IN cord-290231-4m9lj0uq 25 14 neutralizing neutralize VBG cord-290231-4m9lj0uq 25 15 , , , cord-290231-4m9lj0uq 25 16 hemagglutination hemagglutination NN cord-290231-4m9lj0uq 25 17 - - HYPH cord-290231-4m9lj0uq 25 18 inhibiting inhibit VBG cord-290231-4m9lj0uq 25 19 , , , cord-290231-4m9lj0uq 25 20 and and CC cord-290231-4m9lj0uq 25 21 protective protective JJ cord-290231-4m9lj0uq 25 22 antibodies antibody NNS cord-290231-4m9lj0uq 25 23 , , , cord-290231-4m9lj0uq 25 24 it -PRON- PRP cord-290231-4m9lj0uq 25 25 is be VBZ cord-290231-4m9lj0uq 25 26 the the DT cord-290231-4m9lj0uq 25 27 receptor receptor NN cord-290231-4m9lj0uq 25 28 - - HYPH cord-290231-4m9lj0uq 25 29 binding bind VBG cord-290231-4m9lj0uq 25 30 protein protein NN cord-290231-4m9lj0uq 25 31 and and CC cord-290231-4m9lj0uq 25 32 is be VBZ cord-290231-4m9lj0uq 25 33 also also RB cord-290231-4m9lj0uq 25 34 involved involve VBN cord-290231-4m9lj0uq 25 35 in in IN cord-290231-4m9lj0uq 25 36 acid acid NN cord-290231-4m9lj0uq 25 37 - - HYPH cord-290231-4m9lj0uq 25 38 catalyzed catalyze VBN cord-290231-4m9lj0uq 25 39 membrane membrane NN cord-290231-4m9lj0uq 25 40 fusion fusion NN cord-290231-4m9lj0uq 25 41 ( ( -LRB- cord-290231-4m9lj0uq 25 42 Heinz Heinz NNP cord-290231-4m9lj0uq 25 43 and and CC cord-290231-4m9lj0uq 25 44 Roehrig Roehrig NNP cord-290231-4m9lj0uq 25 45 , , , cord-290231-4m9lj0uq 25 46 1990 1990 CD cord-290231-4m9lj0uq 25 47 ; ; : cord-290231-4m9lj0uq 26 1 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 26 2 et et NNP cord-290231-4m9lj0uq 26 3 al al NNP cord-290231-4m9lj0uq 26 4 . . NNP cord-290231-4m9lj0uq 26 5 , , , cord-290231-4m9lj0uq 26 6 1991 1991 CD cord-290231-4m9lj0uq 26 7 ) ) -RRB- cord-290231-4m9lj0uq 26 8 . . . cord-290231-4m9lj0uq 27 1 Little little JJ cord-290231-4m9lj0uq 27 2 is be VBZ cord-290231-4m9lj0uq 27 3 known know VBN cord-290231-4m9lj0uq 27 4 about about IN cord-290231-4m9lj0uq 27 5 the the DT cord-290231-4m9lj0uq 27 6 function function NN cord-290231-4m9lj0uq 27 7 of of IN cord-290231-4m9lj0uq 27 8 the the DT cord-290231-4m9lj0uq 27 9 M M NNP cord-290231-4m9lj0uq 27 10 protein protein NN cord-290231-4m9lj0uq 27 11 . . . cord-290231-4m9lj0uq 28 1 The the DT cord-290231-4m9lj0uq 28 2 M M NNP cord-290231-4m9lj0uq 28 3 protein protein NN cord-290231-4m9lj0uq 28 4 is be VBZ cord-290231-4m9lj0uq 28 5 cleaved cleave VBN cord-290231-4m9lj0uq 28 6 from from IN cord-290231-4m9lj0uq 28 7 its -PRON- PRP$ cord-290231-4m9lj0uq 28 8 precursor precursor NN cord-290231-4m9lj0uq 28 9 protein protein NN cord-290231-4m9lj0uq 28 10 ( ( -LRB- cord-290231-4m9lj0uq 28 11 prM prM NNP cord-290231-4m9lj0uq 28 12 ) ) -RRB- cord-290231-4m9lj0uq 28 13 after after IN cord-290231-4m9lj0uq 28 14 the the DT cord-290231-4m9lj0uq 28 15 consensus consensus NN cord-290231-4m9lj0uq 28 16 sequence sequence NN cord-290231-4m9lj0uq 28 17 R r NN cord-290231-4m9lj0uq 28 18 - - HYPH cord-290231-4m9lj0uq 28 19 X x NN cord-290231-4m9lj0uq 28 20 - - HYPH cord-290231-4m9lj0uq 28 21 R R NNP cord-290231-4m9lj0uq 28 22 / / SYM cord-290231-4m9lj0uq 28 23 K k NN cord-290231-4m9lj0uq 28 24 - - HYPH cord-290231-4m9lj0uq 28 25 R R NNP cord-290231-4m9lj0uq 28 26 shortly shortly RB cord-290231-4m9lj0uq 28 27 before before IN cord-290231-4m9lj0uq 28 28 or or CC cord-290231-4m9lj0uq 28 29 after after IN cord-290231-4m9lj0uq 28 30 virus virus NN cord-290231-4m9lj0uq 28 31 release release NN cord-290231-4m9lj0uq 28 32 Westaway Westaway NNP cord-290231-4m9lj0uq 28 33 , , , cord-290231-4m9lj0uq 28 34 1987 1987 CD cord-290231-4m9lj0uq 28 35 ) ) -RRB- cord-290231-4m9lj0uq 28 36 . . . cord-290231-4m9lj0uq 29 1 This this DT cord-290231-4m9lj0uq 29 2 cleavage cleavage NN cord-290231-4m9lj0uq 29 3 is be VBZ cord-290231-4m9lj0uq 29 4 associated associate VBN cord-290231-4m9lj0uq 29 5 with with IN cord-290231-4m9lj0uq 29 6 a a DT cord-290231-4m9lj0uq 29 7 rearrangement rearrangement NN cord-290231-4m9lj0uq 29 8 of of IN cord-290231-4m9lj0uq 29 9 the the DT cord-290231-4m9lj0uq 29 10 oligomeric oligomeric JJ cord-290231-4m9lj0uq 29 11 structures structure NNS cord-290231-4m9lj0uq 29 12 on on IN cord-290231-4m9lj0uq 29 13 the the DT cord-290231-4m9lj0uq 29 14 surface surface NN cord-290231-4m9lj0uq 29 15 of of IN cord-290231-4m9lj0uq 29 16 the the DT cord-290231-4m9lj0uq 29 17 virion virion NN cord-290231-4m9lj0uq 29 18 ( ( -LRB- cord-290231-4m9lj0uq 29 19 Wengler Wengler NNP cord-290231-4m9lj0uq 29 20 and and CC cord-290231-4m9lj0uq 29 21 Wengler Wengler NNP cord-290231-4m9lj0uq 29 22 , , , cord-290231-4m9lj0uq 29 23 1989 1989 CD cord-290231-4m9lj0uq 29 24 ) ) -RRB- cord-290231-4m9lj0uq 29 25 . . . cord-290231-4m9lj0uq 30 1 By by IN cord-290231-4m9lj0uq 30 2 using use VBG cord-290231-4m9lj0uq 30 3 monoclonal monoclonal JJ cord-290231-4m9lj0uq 30 4 antibodies antibody NNS cord-290231-4m9lj0uq 30 5 ( ( -LRB- cord-290231-4m9lj0uq 30 6 MAbs MAbs NNP cord-290231-4m9lj0uq 30 7 ) ) -RRB- cord-290231-4m9lj0uq 30 8 and and CC cord-290231-4m9lj0uq 30 9 protease protease NN cord-290231-4m9lj0uq 30 10 maps map NNS cord-290231-4m9lj0uq 30 11 , , , cord-290231-4m9lj0uq 30 12 we -PRON- PRP cord-290231-4m9lj0uq 30 13 previously previously RB cord-290231-4m9lj0uq 30 14 demonstrated demonstrate VBD cord-290231-4m9lj0uq 30 15 that that IN cord-290231-4m9lj0uq 30 16 the the DT cord-290231-4m9lj0uq 30 17 E e NN cord-290231-4m9lj0uq 30 18 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 30 19 of of IN cord-290231-4m9lj0uq 30 20 tick tick NN cord-290231-4m9lj0uq 30 21 - - HYPH cord-290231-4m9lj0uq 30 22 borne bear VBN cord-290231-4m9lj0uq 30 23 encephalitis encephalitis JJ cord-290231-4m9lj0uq 30 24 ( ( -LRB- cord-290231-4m9lj0uq 30 25 TBE TBE NNP cord-290231-4m9lj0uq 30 26 ) ) -RRB- cord-290231-4m9lj0uq 30 27 virus virus NN cord-290231-4m9lj0uq 30 28 undergoes undergo VBZ cord-290231-4m9lj0uq 30 29 an an DT cord-290231-4m9lj0uq 30 30 irreversible irreversible JJ cord-290231-4m9lj0uq 30 31 conformational conformational JJ cord-290231-4m9lj0uq 30 32 change change NN cord-290231-4m9lj0uq 30 33 , , , cord-290231-4m9lj0uq 30 34 predominantly predominantly RB cord-290231-4m9lj0uq 30 35 in in IN cord-290231-4m9lj0uq 30 36 the the DT cord-290231-4m9lj0uq 30 37 epitopes epitope NNS cord-290231-4m9lj0uq 30 38 of of IN cord-290231-4m9lj0uq 30 39 domain domain NN cord-290231-4m9lj0uq 30 40 A A NNP cord-290231-4m9lj0uq 30 41 , , , cord-290231-4m9lj0uq 30 42 at at IN cord-290231-4m9lj0uq 30 43 mildly mildly RB cord-290231-4m9lj0uq 30 44 acidic acidic JJ cord-290231-4m9lj0uq 30 45 pH ph NN cord-290231-4m9lj0uq 30 46 . . . cord-290231-4m9lj0uq 31 1 Consistent consistent JJ cord-290231-4m9lj0uq 31 2 with with IN cord-290231-4m9lj0uq 31 3 this this DT cord-290231-4m9lj0uq 31 4 , , , cord-290231-4m9lj0uq 31 5 purified purify VBN cord-290231-4m9lj0uq 31 6 TBE TBE NNP cord-290231-4m9lj0uq 31 7 virus virus NN cord-290231-4m9lj0uq 31 8 demonstrated demonstrate VBD cord-290231-4m9lj0uq 31 9 fusion fusion NN cord-290231-4m9lj0uq 31 10 from from IN cord-290231-4m9lj0uq 31 11 without without IN cord-290231-4m9lj0uq 31 12 ( ( -LRB- cord-290231-4m9lj0uq 31 13 FFWO FFWO NNP cord-290231-4m9lj0uq 31 14 ) ) -RRB- cord-290231-4m9lj0uq 31 15 of of IN cord-290231-4m9lj0uq 31 16 the the DT cord-290231-4m9lj0uq 31 17 mosquito mosquito NN cord-290231-4m9lj0uq 31 18 cell cell NN cord-290231-4m9lj0uq 31 19 line line NN cord-290231-4m9lj0uq 31 20 C6/36 C6/36 NNP cord-290231-4m9lj0uq 31 21 only only RB cord-290231-4m9lj0uq 31 22 after after IN cord-290231-4m9lj0uq 31 23 exposure exposure NN cord-290231-4m9lj0uq 31 24 of of IN cord-290231-4m9lj0uq 31 25 cell cell NN cord-290231-4m9lj0uq 31 26 - - HYPH cord-290231-4m9lj0uq 31 27 bound bind VBN cord-290231-4m9lj0uq 31 28 virus virus NN cord-290231-4m9lj0uq 31 29 to to IN cord-290231-4m9lj0uq 31 30 low low JJ cord-290231-4m9lj0uq 31 31 pH. ph. NN cord-290231-4m9lj0uq 32 1 The the DT cord-290231-4m9lj0uq 32 2 FFWO FFWO NNP cord-290231-4m9lj0uq 32 3 was be VBD cord-290231-4m9lj0uq 32 4 abolished abolish VBN cord-290231-4m9lj0uq 32 5 if if IN cord-290231-4m9lj0uq 32 6 the the DT cord-290231-4m9lj0uq 32 7 processing processing NN cord-290231-4m9lj0uq 32 8 of of IN cord-290231-4m9lj0uq 32 9 prM prm JJ cord-290231-4m9lj0uq 32 10 to to IN cord-290231-4m9lj0uq 32 11 M M NNP cord-290231-4m9lj0uq 32 12 was be VBD cord-290231-4m9lj0uq 32 13 interrupted interrupt VBN cord-290231-4m9lj0uq 32 14 using use VBG cord-290231-4m9lj0uq 32 15 the the DT cord-290231-4m9lj0uq 32 16 acidotropic acidotropic NNP cord-290231-4m9lj0uq 32 17 agent agent NN cord-290231-4m9lj0uq 32 18 ammonium ammonium NN cord-290231-4m9lj0uq 32 19 chloride chloride NN cord-290231-4m9lj0uq 32 20 ( ( -LRB- cord-290231-4m9lj0uq 32 21 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 32 22 et et NNP cord-290231-4m9lj0uq 32 23 a/. a/. NN cord-290231-4m9lj0uq 32 24 , , , cord-290231-4m9lj0uq 32 25 1991 1991 CD cord-290231-4m9lj0uq 32 26 ) ) -RRB- cord-290231-4m9lj0uq 32 27 . . . cord-290231-4m9lj0uq 33 1 In in IN cord-290231-4m9lj0uq 33 2 this this DT cord-290231-4m9lj0uq 33 3 paper paper NN cord-290231-4m9lj0uq 33 4 we -PRON- PRP cord-290231-4m9lj0uq 33 5 identify identify VBP cord-290231-4m9lj0uq 33 6 those those DT cord-290231-4m9lj0uq 33 7 epitopes epitope NNS cord-290231-4m9lj0uq 33 8 on on IN cord-290231-4m9lj0uq 33 9 the the DT cord-290231-4m9lj0uq 33 10 E e NN cord-290231-4m9lj0uq 33 11 protein protein NN cord-290231-4m9lj0uq 33 12 which which WDT cord-290231-4m9lj0uq 33 13 are be VBP cord-290231-4m9lj0uq 33 14 covered cover VBN cord-290231-4m9lj0uq 33 15 or or CC cord-290231-4m9lj0uq 33 16 dissociated dissociate VBN cord-290231-4m9lj0uq 33 17 by by IN cord-290231-4m9lj0uq 33 18 the the DT cord-290231-4m9lj0uq 33 19 presence presence NN cord-290231-4m9lj0uq 33 20 of of IN cord-290231-4m9lj0uq 33 21 prM prm JJ cord-290231-4m9lj0uq 33 22 , , , cord-290231-4m9lj0uq 33 23 demonstrate demonstrate VBP cord-290231-4m9lj0uq 33 24 that that IN cord-290231-4m9lj0uq 33 25 prM prm NN cord-290231-4m9lj0uq 33 26 - - HYPH cord-290231-4m9lj0uq 33 27 containing contain VBG cord-290231-4m9lj0uq 33 28 viruses virus NNS cord-290231-4m9lj0uq 33 29 are be VBP cord-290231-4m9lj0uq 33 30 more more RBR cord-290231-4m9lj0uq 33 31 resistant resistant JJ cord-290231-4m9lj0uq 33 32 to to IN cord-290231-4m9lj0uq 33 33 low low JJ cord-290231-4m9lj0uq 33 34 pH ph NN cord-290231-4m9lj0uq 33 35 , , , cord-290231-4m9lj0uq 33 36 localize localize VB cord-290231-4m9lj0uq 33 37 the the DT cord-290231-4m9lj0uq 33 38 site site NN cord-290231-4m9lj0uq 33 39 on on IN cord-290231-4m9lj0uq 33 40 the the DT cord-290231-4m9lj0uq 33 41 E e NN cord-290231-4m9lj0uq 33 42 protein protein NN cord-290231-4m9lj0uq 33 43 which which WDT cord-290231-4m9lj0uq 33 44 is be VBZ cord-290231-4m9lj0uq 33 45 involved involve VBN cord-290231-4m9lj0uq 33 46 in in IN cord-290231-4m9lj0uq 33 47 the the DT cord-290231-4m9lj0uq 33 48 E e JJ cord-290231-4m9lj0uq 33 49 - - JJ cord-290231-4m9lj0uq 33 50 prM prm JJ cord-290231-4m9lj0uq 33 51 interaction interaction NN cord-290231-4m9lj0uq 33 52 , , , cord-290231-4m9lj0uq 33 53 and and CC cord-290231-4m9lj0uq 33 54 finally finally RB cord-290231-4m9lj0uq 33 55 hypothesize hypothesize VB cord-290231-4m9lj0uq 33 56 that that IN cord-290231-4m9lj0uq 33 57 the the DT cord-290231-4m9lj0uq 33 58 association association NN cord-290231-4m9lj0uq 33 59 of of IN cord-290231-4m9lj0uq 33 60 E E NNP cord-290231-4m9lj0uq 33 61 - - NN cord-290231-4m9lj0uq 33 62 prM prm JJ cord-290231-4m9lj0uq 33 63 is be VBZ cord-290231-4m9lj0uq 33 64 necessary necessary JJ cord-290231-4m9lj0uq 33 65 for for IN cord-290231-4m9lj0uq 33 66 the the DT cord-290231-4m9lj0uq 33 67 authentic authentic JJ cord-290231-4m9lj0uq 33 68 expression expression NN cord-290231-4m9lj0uq 33 69 of of IN cord-290231-4m9lj0uq 33 70 the the DT cord-290231-4m9lj0uq 33 71 E e NN cord-290231-4m9lj0uq 33 72 protein protein NN cord-290231-4m9lj0uq 33 73 . . . cord-290231-4m9lj0uq 34 1 Production production NN cord-290231-4m9lj0uq 34 2 and and CC cord-290231-4m9lj0uq 34 3 analysis analysis NN cord-290231-4m9lj0uq 34 4 of of IN cord-290231-4m9lj0uq 34 5 the the DT cord-290231-4m9lj0uq 34 6 immature immature JJ cord-290231-4m9lj0uq 34 7 virus virus NN cord-290231-4m9lj0uq 34 8 particles particle NNS cord-290231-4m9lj0uq 34 9 Aedes Aedes NNP cord-290231-4m9lj0uq 34 10 albopictus albopictus NN cord-290231-4m9lj0uq 34 11 cells cell NNS cord-290231-4m9lj0uq 34 12 ( ( -LRB- cord-290231-4m9lj0uq 35 1 clone clone NNP cord-290231-4m9lj0uq 35 2 C6/36 C6/36 NNP cord-290231-4m9lj0uq 35 3 ) ) -RRB- cord-290231-4m9lj0uq 35 4 were be VBD cord-290231-4m9lj0uq 35 5 grown grow VBN cord-290231-4m9lj0uq 35 6 on on IN cord-290231-4m9lj0uq 35 7 microcarriers microcarrier NNS cord-290231-4m9lj0uq 35 8 ( ( -LRB- cord-290231-4m9lj0uq 35 9 Cytodex Cytodex NNP cord-290231-4m9lj0uq 35 10 2 2 CD cord-290231-4m9lj0uq 35 11 , , , cord-290231-4m9lj0uq 35 12 Pharmacia Pharmacia NNP cord-290231-4m9lj0uq 35 13 Fine Fine NNP cord-290231-4m9lj0uq 35 14 Chemicals Chemicals NNPS cord-290231-4m9lj0uq 35 15 , , , cord-290231-4m9lj0uq 35 16 Upsala Upsala NNP cord-290231-4m9lj0uq 35 17 , , , cord-290231-4m9lj0uq 35 18 Sweden Sweden NNP cord-290231-4m9lj0uq 35 19 ) ) -RRB- cord-290231-4m9lj0uq 35 20 ' ' '' cord-290231-4m9lj0uq 35 21 in in IN cord-290231-4m9lj0uq 35 22 Dulbecco Dulbecco NNP cord-290231-4m9lj0uq 35 23 's 's POS cord-290231-4m9lj0uq 35 24 minimal minimal JJ cord-290231-4m9lj0uq 35 25 essential essential JJ cord-290231-4m9lj0uq 35 26 medium medium NN cord-290231-4m9lj0uq 35 27 ( ( -LRB- cord-290231-4m9lj0uq 35 28 D D NNP cord-290231-4m9lj0uq 35 29 - - HYPH cord-290231-4m9lj0uq 35 30 MEM mem NN cord-290231-4m9lj0uq 35 31 ) ) -RRB- cord-290231-4m9lj0uq 35 32 buffered buffer VBN cord-290231-4m9lj0uq 35 33 with with IN cord-290231-4m9lj0uq 35 34 25 25 CD cord-290231-4m9lj0uq 35 35 mNI mNI NNS cord-290231-4m9lj0uq 35 36 HEPES HEPES NNP cord-290231-4m9lj0uq 35 37 and and CC cord-290231-4m9lj0uq 35 38 supplemented supplement VBN cord-290231-4m9lj0uq 35 39 with with IN cord-290231-4m9lj0uq 35 40 10 10 CD cord-290231-4m9lj0uq 35 41 % % NN cord-290231-4m9lj0uq 35 42 fetal fetal JJ cord-290231-4m9lj0uq 35 43 calf calf NN cord-290231-4m9lj0uq 35 44 serum serum NN cord-290231-4m9lj0uq 35 45 ( ( -LRB- cord-290231-4m9lj0uq 35 46 FCS FCS NNP cord-290231-4m9lj0uq 35 47 , , , cord-290231-4m9lj0uq 35 48 Hyclone Hyclone NNP cord-290231-4m9lj0uq 35 49 Laboratories Laboratories NNP cord-290231-4m9lj0uq 35 50 , , , cord-290231-4m9lj0uq 35 51 Inc. Inc. NNP cord-290231-4m9lj0uq 35 52 , , , cord-290231-4m9lj0uq 35 53 Logan Logan NNP cord-290231-4m9lj0uq 35 54 , , , cord-290231-4m9lj0uq 35 55 UT UT NNP cord-290231-4m9lj0uq 35 56 ) ) -RRB- cord-290231-4m9lj0uq 35 57 . . . cord-290231-4m9lj0uq 36 1 Cells cell NNS cord-290231-4m9lj0uq 36 2 were be VBD cord-290231-4m9lj0uq 36 3 infected infect VBN cord-290231-4m9lj0uq 36 4 with with IN cord-290231-4m9lj0uq 36 5 MVE MVE NNP cord-290231-4m9lj0uq 36 6 virus virus NN cord-290231-4m9lj0uq 36 7 ( ( -LRB- cord-290231-4m9lj0uq 36 8 strain strain VBP cord-290231-4m9lj0uq 36 9 Ord Ord NNP cord-290231-4m9lj0uq 36 10 River River NNP cord-290231-4m9lj0uq 36 11 ) ) -RRB- cord-290231-4m9lj0uq 36 12 at at IN cord-290231-4m9lj0uq 36 13 a a DT cord-290231-4m9lj0uq 36 14 multiplicity multiplicity NN cord-290231-4m9lj0uq 36 15 of of IN cord-290231-4m9lj0uq 36 16 infection infection NN cord-290231-4m9lj0uq 36 17 ( ( -LRB- cord-290231-4m9lj0uq 36 18 m.o.i m.o.i NNP cord-290231-4m9lj0uq 36 19 . . NNP cord-290231-4m9lj0uq 36 20 ) ) -RRB- cord-290231-4m9lj0uq 36 21 of of IN cord-290231-4m9lj0uq 36 22 10 10 CD cord-290231-4m9lj0uq 36 23 in in IN cord-290231-4m9lj0uq 36 24 D D NNP cord-290231-4m9lj0uq 36 25 - - HYPH cord-290231-4m9lj0uq 36 26 MEM mem NN cord-290231-4m9lj0uq 36 27 with with IN cord-290231-4m9lj0uq 36 28 29 29 CD cord-290231-4m9lj0uq 36 29 / / SYM cord-290231-4m9lj0uq 36 30 o o NN cord-290231-4m9lj0uq 36 31 FCS FCS NNP cord-290231-4m9lj0uq 36 32 . . . cord-290231-4m9lj0uq 37 1 Ammonium ammonium NN cord-290231-4m9lj0uq 37 2 chloride chloride NN cord-290231-4m9lj0uq 37 3 ( ( -LRB- cord-290231-4m9lj0uq 37 4 25 25 CD cord-290231-4m9lj0uq 37 5 m m NN cord-290231-4m9lj0uq 37 6 / / SYM cord-290231-4m9lj0uq 37 7 M M NNP cord-290231-4m9lj0uq 37 8 ) ) -RRB- cord-290231-4m9lj0uq 37 9 was be VBD cord-290231-4m9lj0uq 37 10 added add VBN cord-290231-4m9lj0uq 37 11 at at IN cord-290231-4m9lj0uq 37 12 48 48 CD cord-290231-4m9lj0uq 37 13 hr hr NN cord-290231-4m9lj0uq 37 14 postinfection postinfection NN cord-290231-4m9lj0uq 37 15 ( ( -LRB- cord-290231-4m9lj0uq 37 16 p.i p.i NNP cord-290231-4m9lj0uq 37 17 . . NNP cord-290231-4m9lj0uq 37 18 ) ) -RRB- cord-290231-4m9lj0uq 37 19 and and CC cord-290231-4m9lj0uq 37 20 the the DT cord-290231-4m9lj0uq 37 21 viruses virus NNS cord-290231-4m9lj0uq 37 22 were be VBD cord-290231-4m9lj0uq 37 23 harvested harvest VBN cord-290231-4m9lj0uq 37 24 on on IN cord-290231-4m9lj0uq 37 25 Day Day NNP cord-290231-4m9lj0uq 37 26 6 6 CD cord-290231-4m9lj0uq 37 27 . . . cord-290231-4m9lj0uq 38 1 At at IN cord-290231-4m9lj0uq 38 2 this this DT cord-290231-4m9lj0uq 38 3 time time NN cord-290231-4m9lj0uq 38 4 , , , cord-290231-4m9lj0uq 38 5 ammonium ammonium NN cord-290231-4m9lj0uq 38 6 chloride chloride NN cord-290231-4m9lj0uq 38 7 - - HYPH cord-290231-4m9lj0uq 38 8 containing contain VBG cord-290231-4m9lj0uq 38 9 medium medium NN cord-290231-4m9lj0uq 38 10 was be VBD cord-290231-4m9lj0uq 38 11 replaced replace VBN cord-290231-4m9lj0uq 38 12 with with IN cord-290231-4m9lj0uq 38 13 D D NNP cord-290231-4m9lj0uq 38 14 - - HYPH cord-290231-4m9lj0uq 38 15 MEM mem NN cord-290231-4m9lj0uq 38 16 , , , cord-290231-4m9lj0uq 38 17 2 2 CD cord-290231-4m9lj0uq 38 18 % % NN cord-290231-4m9lj0uq 38 19 FCS FCS NNP cord-290231-4m9lj0uq 38 20 , , , cord-290231-4m9lj0uq 38 21 without without IN cord-290231-4m9lj0uq 38 22 ammonium ammonium NN cord-290231-4m9lj0uq 38 23 chloride chloride NN cord-290231-4m9lj0uq 38 24 , , , cord-290231-4m9lj0uq 38 25 and and CC cord-290231-4m9lj0uq 38 26 the the DT cord-290231-4m9lj0uq 38 27 viruses virus NNS cord-290231-4m9lj0uq 38 28 were be VBD cord-290231-4m9lj0uq 38 29 reharvested reharveste VBN cord-290231-4m9lj0uq 38 30 on on IN cord-290231-4m9lj0uq 38 31 Day Day NNP cord-290231-4m9lj0uq 38 32 9 9 CD cord-290231-4m9lj0uq 38 33 p.i p.i NNP cord-290231-4m9lj0uq 38 34 . . . cord-290231-4m9lj0uq 38 35 Viruses virus NNS cord-290231-4m9lj0uq 38 36 were be VBD cord-290231-4m9lj0uq 38 37 precipitated precipitate VBN cord-290231-4m9lj0uq 38 38 with with IN cord-290231-4m9lj0uq 38 39 8 8 CD cord-290231-4m9lj0uq 38 40 % % NN cord-290231-4m9lj0uq 38 41 polyethylene polyethylene NN cord-290231-4m9lj0uq 38 42 glycol glycol NN cord-290231-4m9lj0uq 38 43 8000 8000 CD cord-290231-4m9lj0uq 39 1 ( ( -LRB- cord-290231-4m9lj0uq 39 2 Fisher Fisher NNP cord-290231-4m9lj0uq 39 3 Scientific Scientific NNP cord-290231-4m9lj0uq 39 4 , , , cord-290231-4m9lj0uq 39 5 Fair Fair NNP cord-290231-4m9lj0uq 39 6 Lawn Lawn NNP cord-290231-4m9lj0uq 39 7 , , , cord-290231-4m9lj0uq 39 8 NJ NJ NNP cord-290231-4m9lj0uq 39 9 ) ) -RRB- cord-290231-4m9lj0uq 39 10 , , , cord-290231-4m9lj0uq 39 11 purified purify VBN cord-290231-4m9lj0uq 39 12 by by IN cord-290231-4m9lj0uq 39 13 two two CD cord-290231-4m9lj0uq 39 14 cycles cycle NNS cord-290231-4m9lj0uq 39 15 of of IN cord-290231-4m9lj0uq 39 16 glycerol glycerol NN cord-290231-4m9lj0uq 39 17 - - HYPH cord-290231-4m9lj0uq 39 18 tartrate tartrate NN cord-290231-4m9lj0uq 39 19 gradients gradient NNS cord-290231-4m9lj0uq 39 20 ( ( -LRB- cord-290231-4m9lj0uq 39 21 Obijeski Obijeski NNP cord-290231-4m9lj0uq 39 22 et et NNP cord-290231-4m9lj0uq 39 23 al al NNP cord-290231-4m9lj0uq 39 24 . . NNP cord-290231-4m9lj0uq 39 25 , , , cord-290231-4m9lj0uq 39 26 1976 1976 CD cord-290231-4m9lj0uq 39 27 ) ) -RRB- cord-290231-4m9lj0uq 39 28 , , , cord-290231-4m9lj0uq 39 29 and and CC cord-290231-4m9lj0uq 39 30 resuspended resuspend VBD cord-290231-4m9lj0uq 39 31 in in IN cord-290231-4m9lj0uq 39 32 0.3 0.3 CD cord-290231-4m9lj0uq 39 33 ml ml NNS cord-290231-4m9lj0uq 39 34 of of IN cord-290231-4m9lj0uq 39 35 D D NNP cord-290231-4m9lj0uq 39 36 - - HYPH cord-290231-4m9lj0uq 39 37 MEM mem NN cord-290231-4m9lj0uq 39 38 . . . cord-290231-4m9lj0uq 40 1 Protein protein NN cord-290231-4m9lj0uq 40 2 concentrations concentration NNS cord-290231-4m9lj0uq 40 3 were be VBD cord-290231-4m9lj0uq 40 4 determined determine VBN cord-290231-4m9lj0uq 40 5 by by IN cord-290231-4m9lj0uq 40 6 the the DT cord-290231-4m9lj0uq 40 7 Bio Bio NNP cord-290231-4m9lj0uq 40 8 - - HYPH cord-290231-4m9lj0uq 40 9 Rad Rad NNP cord-290231-4m9lj0uq 40 10 protein protein NN cord-290231-4m9lj0uq 40 11 assay assay NN cord-290231-4m9lj0uq 40 12 ( ( -LRB- cord-290231-4m9lj0uq 40 13 Bio Bio NNP cord-290231-4m9lj0uq 40 14 - - HYPH cord-290231-4m9lj0uq 40 15 Rad Rad NNP cord-290231-4m9lj0uq 40 16 Laboratories Laboratories NNP cord-290231-4m9lj0uq 40 17 , , , cord-290231-4m9lj0uq 40 18 Richmond Richmond NNP cord-290231-4m9lj0uq 40 19 , , , cord-290231-4m9lj0uq 40 20 CA CA NNP cord-290231-4m9lj0uq 40 21 ) ) -RRB- cord-290231-4m9lj0uq 40 22 . . . cord-290231-4m9lj0uq 41 1 Untreated untreated JJ cord-290231-4m9lj0uq 41 2 MVE MVE NNP cord-290231-4m9lj0uq 41 3 viruses virus NNS cord-290231-4m9lj0uq 41 4 ( ( -LRB- cord-290231-4m9lj0uq 41 5 without without IN cord-290231-4m9lj0uq 41 6 ammonium ammonium NN cord-290231-4m9lj0uq 41 7 chloride chloride NN cord-290231-4m9lj0uq 41 8 ) ) -RRB- cord-290231-4m9lj0uq 41 9 were be VBD cord-290231-4m9lj0uq 41 10 propagated propagate VBN cord-290231-4m9lj0uq 41 11 and and CC cord-290231-4m9lj0uq 41 12 purified purify VBN cord-290231-4m9lj0uq 41 13 as as IN cord-290231-4m9lj0uq 41 14 described describe VBN cord-290231-4m9lj0uq 41 15 above above RB cord-290231-4m9lj0uq 41 16 and and CC cord-290231-4m9lj0uq 41 17 were be VBD cord-290231-4m9lj0uq 41 18 used use VBN cord-290231-4m9lj0uq 41 19 as as IN cord-290231-4m9lj0uq 41 20 positive positive JJ cord-290231-4m9lj0uq 41 21 controls control NNS cord-290231-4m9lj0uq 41 22 in in IN cord-290231-4m9lj0uq 41 23 all all DT cord-290231-4m9lj0uq 41 24 experiments experiment NNS cord-290231-4m9lj0uq 41 25 . . . cord-290231-4m9lj0uq 42 1 For for IN cord-290231-4m9lj0uq 42 2 the the DT cord-290231-4m9lj0uq 42 3 acid acid NN cord-290231-4m9lj0uq 42 4 resistance resistance NN cord-290231-4m9lj0uq 42 5 experiments experiment NNS cord-290231-4m9lj0uq 42 6 ( ( -LRB- cord-290231-4m9lj0uq 42 7 see see VB cord-290231-4m9lj0uq 42 8 below below RB cord-290231-4m9lj0uq 42 9 ) ) -RRB- cord-290231-4m9lj0uq 43 1 MVE mve NN cord-290231-4m9lj0uq 43 2 viruses virus NNS cord-290231-4m9lj0uq 43 3 were be VBD cord-290231-4m9lj0uq 43 4 also also RB cord-290231-4m9lj0uq 43 5 grown grow VBN cord-290231-4m9lj0uq 43 6 in in IN cord-290231-4m9lj0uq 43 7 SW1 SW1 NNP cord-290231-4m9lj0uq 43 8 3 3 CD cord-290231-4m9lj0uq 43 9 mammalian mammalian JJ cord-290231-4m9lj0uq 43 10 cells cell NNS cord-290231-4m9lj0uq 43 11 in in IN cord-290231-4m9lj0uq 43 12 the the DT cord-290231-4m9lj0uq 43 13 presence presence NN cord-290231-4m9lj0uq 43 14 or or CC cord-290231-4m9lj0uq 43 15 absence absence NN cord-290231-4m9lj0uq 43 16 of of IN cord-290231-4m9lj0uq 43 17 ammonium ammonium NN cord-290231-4m9lj0uq 43 18 chloride chloride NN cord-290231-4m9lj0uq 43 19 . . . cord-290231-4m9lj0uq 44 1 To to TO cord-290231-4m9lj0uq 44 2 quantify quantify VB cord-290231-4m9lj0uq 44 3 the the DT cord-290231-4m9lj0uq 44 4 amount amount NN cord-290231-4m9lj0uq 44 5 of of IN cord-290231-4m9lj0uq 44 6 viral viral JJ cord-290231-4m9lj0uq 44 7 structural structural JJ cord-290231-4m9lj0uq 44 8 proteins protein NNS cord-290231-4m9lj0uq 44 9 in in IN cord-290231-4m9lj0uq 44 10 each each DT cord-290231-4m9lj0uq 44 11 propagation propagation NN cord-290231-4m9lj0uq 44 12 , , , cord-290231-4m9lj0uq 44 13 the the DT cord-290231-4m9lj0uq 44 14 purified purify VBN cord-290231-4m9lj0uq 44 15 viruses virus NNS cord-290231-4m9lj0uq 44 16 were be VBD cord-290231-4m9lj0uq 44 17 subjected subject VBN cord-290231-4m9lj0uq 44 18 to to IN cord-290231-4m9lj0uq 44 19 SDS SDS NNP cord-290231-4m9lj0uq 44 20 - - HYPH cord-290231-4m9lj0uq 44 21 PAGE PAGE NNP cord-290231-4m9lj0uq 44 22 on on IN cord-290231-4m9lj0uq 44 23 12.5 12.5 CD cord-290231-4m9lj0uq 44 24 % % NN cord-290231-4m9lj0uq 44 25 acryamide acryamide JJ cord-290231-4m9lj0uq 44 26 gels gel NNS cord-290231-4m9lj0uq 44 27 ( ( -LRB- cord-290231-4m9lj0uq 44 28 Laemmli Laemmli NNP cord-290231-4m9lj0uq 44 29 , , , cord-290231-4m9lj0uq 44 30 1970 1970 CD cord-290231-4m9lj0uq 44 31 ) ) -RRB- cord-290231-4m9lj0uq 44 32 and and CC cord-290231-4m9lj0uq 44 33 proteins protein NNS cord-290231-4m9lj0uq 44 34 were be VBD cord-290231-4m9lj0uq 44 35 stained stain VBN cord-290231-4m9lj0uq 44 36 using use VBG cord-290231-4m9lj0uq 44 37 a a DT cord-290231-4m9lj0uq 44 38 rapid rapid JJ cord-290231-4m9lj0uq 44 39 silver silver NN cord-290231-4m9lj0uq 44 40 stain stain NN cord-290231-4m9lj0uq 44 41 kit kit NN cord-290231-4m9lj0uq 44 42 ( ( -LRB- cord-290231-4m9lj0uq 44 43 ICN ICN NNP cord-290231-4m9lj0uq 44 44 , , , cord-290231-4m9lj0uq 44 45 Cleveland Cleveland NNP cord-290231-4m9lj0uq 44 46 , , , cord-290231-4m9lj0uq 44 47 OH OH NNP cord-290231-4m9lj0uq 44 48 ) ) -RRB- cord-290231-4m9lj0uq 44 49 according accord VBG cord-290231-4m9lj0uq 44 50 to to IN cord-290231-4m9lj0uq 44 51 the the DT cord-290231-4m9lj0uq 44 52 manufacturer manufacturer NN cord-290231-4m9lj0uq 44 53 's 's POS cord-290231-4m9lj0uq 44 54 protocols protocol NNS cord-290231-4m9lj0uq 44 55 . . . cord-290231-4m9lj0uq 45 1 Gels gel NNS cord-290231-4m9lj0uq 45 2 were be VBD cord-290231-4m9lj0uq 45 3 scanned scan VBN cord-290231-4m9lj0uq 45 4 by by IN cord-290231-4m9lj0uq 45 5 a a DT cord-290231-4m9lj0uq 45 6 microprocessor microprocessor NN cord-290231-4m9lj0uq 45 7 - - HYPH cord-290231-4m9lj0uq 45 8 controled control VBN cord-290231-4m9lj0uq 45 9 spectrophotometer spectrophotometer NN cord-290231-4m9lj0uq 45 10 ( ( -LRB- cord-290231-4m9lj0uq 45 11 Gilford Gilford NNP cord-290231-4m9lj0uq 45 12 System System NNP cord-290231-4m9lj0uq 45 13 2600 2600 CD cord-290231-4m9lj0uq 45 14 ) ) -RRB- cord-290231-4m9lj0uq 45 15 and and CC cord-290231-4m9lj0uq 45 16 the the DT cord-290231-4m9lj0uq 45 17 peaks peak NNS cord-290231-4m9lj0uq 45 18 corresponding correspond VBG cord-290231-4m9lj0uq 45 19 to to IN cord-290231-4m9lj0uq 45 20 each each DT cord-290231-4m9lj0uq 45 21 viral viral JJ cord-290231-4m9lj0uq 45 22 protein protein NN cord-290231-4m9lj0uq 45 23 were be VBD cord-290231-4m9lj0uq 45 24 identified identify VBN cord-290231-4m9lj0uq 45 25 and and CC cord-290231-4m9lj0uq 45 26 integrated integrate VBN cord-290231-4m9lj0uq 45 27 to to TO cord-290231-4m9lj0uq 45 28 determine determine VB cord-290231-4m9lj0uq 45 29 areas area NNS cord-290231-4m9lj0uq 45 30 under under IN cord-290231-4m9lj0uq 45 31 the the DT cord-290231-4m9lj0uq 45 32 peaks peak NNS cord-290231-4m9lj0uq 45 33 , , , cord-290231-4m9lj0uq 45 34 and and CC cord-290231-4m9lj0uq 45 35 the the DT cord-290231-4m9lj0uq 45 36 area area NN cord-290231-4m9lj0uq 45 37 ratios ratio NNS cord-290231-4m9lj0uq 45 38 of of IN cord-290231-4m9lj0uq 45 39 prM prm JJ cord-290231-4m9lj0uq 45 40 to to IN cord-290231-4m9lj0uq 45 41 E E NNS cord-290231-4m9lj0uq 45 42 were be VBD cord-290231-4m9lj0uq 45 43 calculated calculate VBN cord-290231-4m9lj0uq 45 44 . . . cord-290231-4m9lj0uq 46 1 Purified purify VBN cord-290231-4m9lj0uq 46 2 viruses virus NNS cord-290231-4m9lj0uq 46 3 were be VBD cord-290231-4m9lj0uq 46 4 diluted dilute VBN cord-290231-4m9lj0uq 46 5 geometrically geometrically RB cord-290231-4m9lj0uq 46 6 ( ( -LRB- cord-290231-4m9lj0uq 46 7 from from IN cord-290231-4m9lj0uq 46 8 200 200 CD cord-290231-4m9lj0uq 46 9 to to IN cord-290231-4m9lj0uq 46 10 6.25 6.25 CD cord-290231-4m9lj0uq 46 11 pg pg NNP cord-290231-4m9lj0uq 46 12 / / SYM cord-290231-4m9lj0uq 46 13 mI mI NNP cord-290231-4m9lj0uq 46 14 ) ) -RRB- cord-290231-4m9lj0uq 46 15 in in IN cord-290231-4m9lj0uq 46 16 D D NNP cord-290231-4m9lj0uq 46 17 - - HYPH cord-290231-4m9lj0uq 46 18 MEM mem NN cord-290231-4m9lj0uq 46 19 containing contain VBG cord-290231-4m9lj0uq 46 20 1 1 CD cord-290231-4m9lj0uq 46 21 % % NN cord-290231-4m9lj0uq 46 22 BSA BSA NNP cord-290231-4m9lj0uq 46 23 . . . cord-290231-4m9lj0uq 47 1 Thirty thirty CD cord-290231-4m9lj0uq 47 2 microliters microliter NNS cord-290231-4m9lj0uq 47 3 of of IN cord-290231-4m9lj0uq 47 4 each each DT cord-290231-4m9lj0uq 47 5 dilution dilution NN cord-290231-4m9lj0uq 47 6 was be VBD cord-290231-4m9lj0uq 47 7 added add VBN cord-290231-4m9lj0uq 47 8 to to IN cord-290231-4m9lj0uq 47 9 prechilled prechille VBN cord-290231-4m9lj0uq 47 10 C6/ C6/ NNP cord-290231-4m9lj0uq 47 11 36 36 CD cord-290231-4m9lj0uq 47 12 cells cell NNS cord-290231-4m9lj0uq 47 13 and and CC cord-290231-4m9lj0uq 47 14 the the DT cord-290231-4m9lj0uq 47 15 fusion fusion NN cord-290231-4m9lj0uq 47 16 assay assay NN cord-290231-4m9lj0uq 47 17 was be VBD cord-290231-4m9lj0uq 47 18 performed perform VBN cord-290231-4m9lj0uq 47 19 at at IN cord-290231-4m9lj0uq 47 20 pH pH NNP cord-290231-4m9lj0uq 47 21 5.0 5.0 CD cord-290231-4m9lj0uq 47 22 . . . cord-290231-4m9lj0uq 48 1 The the DT cord-290231-4m9lj0uq 48 2 number number NN cord-290231-4m9lj0uq 48 3 of of IN cord-290231-4m9lj0uq 48 4 nuclei nucleus NNS cord-290231-4m9lj0uq 48 5 and and CC cord-290231-4m9lj0uq 48 6 the the DT cord-290231-4m9lj0uq 48 7 number number NN cord-290231-4m9lj0uq 48 8 of of IN cord-290231-4m9lj0uq 48 9 cells cell NNS cord-290231-4m9lj0uq 48 10 in in IN cord-290231-4m9lj0uq 48 11 five five CD cord-290231-4m9lj0uq 48 12 microscopic microscopic JJ cord-290231-4m9lj0uq 48 13 fields field NNS cord-290231-4m9lj0uq 48 14 ( ( -LRB- cord-290231-4m9lj0uq 48 15 magnification magnification NN cord-290231-4m9lj0uq 48 16 1 1 CD cord-290231-4m9lj0uq 48 17 OO oo NN cord-290231-4m9lj0uq 48 18 - - HYPH cord-290231-4m9lj0uq 48 19 fold fold NN cord-290231-4m9lj0uq 48 20 ) ) -RRB- cord-290231-4m9lj0uq 48 21 were be VBD cord-290231-4m9lj0uq 48 22 counted count VBN cord-290231-4m9lj0uq 48 23 and and CC cord-290231-4m9lj0uq 48 24 the the DT cord-290231-4m9lj0uq 48 25 fusion fusion NN cord-290231-4m9lj0uq 48 26 index index NN cord-290231-4m9lj0uq 48 27 [ [ -LRB- cord-290231-4m9lj0uq 48 28 l l NNP cord-290231-4m9lj0uq 48 29 -(number -(number NNP cord-290231-4m9lj0uq 48 30 of of IN cord-290231-4m9lj0uq 48 31 cells/ cells/ CD cord-290231-4m9lj0uq 48 32 number number NN cord-290231-4m9lj0uq 48 33 of of IN cord-290231-4m9lj0uq 48 34 nuclei nucleus NNS cord-290231-4m9lj0uq 48 35 ) ) -RRB- cord-290231-4m9lj0uq 48 36 ] ] -RRB- cord-290231-4m9lj0uq 48 37 was be VBD cord-290231-4m9lj0uq 48 38 calculated calculate VBN cord-290231-4m9lj0uq 48 39 . . . cord-290231-4m9lj0uq 49 1 Capture capture VB cord-290231-4m9lj0uq 49 2 enzyme enzyme NN cord-290231-4m9lj0uq 49 3 - - HYPH cord-290231-4m9lj0uq 49 4 linked link VBN cord-290231-4m9lj0uq 49 5 immunosorbent immunosorbent JJ cord-290231-4m9lj0uq 49 6 assay assay NN cord-290231-4m9lj0uq 49 7 ( ( -LRB- cord-290231-4m9lj0uq 49 8 ELISA ELISA NNP cord-290231-4m9lj0uq 49 9 ) ) -RRB- cord-290231-4m9lj0uq 50 1 Microtiter microtiter JJ cord-290231-4m9lj0uq 50 2 plates plate NNS cord-290231-4m9lj0uq 50 3 were be VBD cord-290231-4m9lj0uq 50 4 coated coat VBN cord-290231-4m9lj0uq 50 5 overnight overnight RB cord-290231-4m9lj0uq 50 6 at at IN cord-290231-4m9lj0uq 50 7 4 4 CD cord-290231-4m9lj0uq 50 8 " " `` cord-290231-4m9lj0uq 50 9 with with IN cord-290231-4m9lj0uq 50 10 a a DT cord-290231-4m9lj0uq 50 11 1:500 1:500 CD cord-290231-4m9lj0uq 50 12 dilution dilution NN cord-290231-4m9lj0uq 50 13 of of IN cord-290231-4m9lj0uq 50 14 rabbit rabbit NN cord-290231-4m9lj0uq 50 15 anti anti NNP cord-290231-4m9lj0uq 50 16 - - NNP cord-290231-4m9lj0uq 50 17 MVE MVE NNP cord-290231-4m9lj0uq 50 18 hyperimmune hyperimmune NNP cord-290231-4m9lj0uq 50 19 serum serum NN cord-290231-4m9lj0uq 50 20 . . . cord-290231-4m9lj0uq 51 1 This this DT cord-290231-4m9lj0uq 51 2 dilution dilution NN cord-290231-4m9lj0uq 51 3 of of IN cord-290231-4m9lj0uq 51 4 capture capture NN cord-290231-4m9lj0uq 51 5 antibody antibody NN cord-290231-4m9lj0uq 51 6 was be VBD cord-290231-4m9lj0uq 51 7 determined determine VBN cord-290231-4m9lj0uq 51 8 by by IN cord-290231-4m9lj0uq 51 9 box box NN cord-290231-4m9lj0uq 51 10 - - HYPH cord-290231-4m9lj0uq 51 11 titration titration NN cord-290231-4m9lj0uq 51 12 against against IN cord-290231-4m9lj0uq 51 13 M M NNP cord-290231-4m9lj0uq 51 14 - - HYPH cord-290231-4m9lj0uq 51 15 and and CC cord-290231-4m9lj0uq 51 16 prM prm JJ cord-290231-4m9lj0uq 51 17 - - HYPH cord-290231-4m9lj0uq 51 18 containing contain VBG cord-290231-4m9lj0uq 51 19 virus virus NN cord-290231-4m9lj0uq 51 20 seeds seed NNS cord-290231-4m9lj0uq 51 21 , , , cord-290231-4m9lj0uq 51 22 maximizing maximize VBG cord-290231-4m9lj0uq 51 23 and and CC cord-290231-4m9lj0uq 51 24 standardizing standardize VBG cord-290231-4m9lj0uq 51 25 the the DT cord-290231-4m9lj0uq 51 26 concentration concentration NN cord-290231-4m9lj0uq 51 27 of of IN cord-290231-4m9lj0uq 51 28 captured capture VBN cord-290231-4m9lj0uq 51 29 antigens antigen NNS cord-290231-4m9lj0uq 51 30 . . . cord-290231-4m9lj0uq 52 1 Wells well NNS cord-290231-4m9lj0uq 52 2 were be VBD cord-290231-4m9lj0uq 52 3 blocked block VBN cord-290231-4m9lj0uq 52 4 with with IN cord-290231-4m9lj0uq 52 5 3 3 CD cord-290231-4m9lj0uq 52 6 % % NN cord-290231-4m9lj0uq 52 7 goat goat NN cord-290231-4m9lj0uq 52 8 serum serum NN cord-290231-4m9lj0uq 52 9 in in IN cord-290231-4m9lj0uq 52 10 PBS PBS NNP cord-290231-4m9lj0uq 52 11 , , , cord-290231-4m9lj0uq 52 12 a a DT cord-290231-4m9lj0uq 52 13 1:5 1:5 CD cord-290231-4m9lj0uq 52 14 dilution dilution NN cord-290231-4m9lj0uq 52 15 of of IN cord-290231-4m9lj0uq 52 16 tissue tissue NN cord-290231-4m9lj0uq 52 17 cultures culture NNS cord-290231-4m9lj0uq 52 18 containing contain VBG cord-290231-4m9lj0uq 52 19 prM prm JJ cord-290231-4m9lj0uq 52 20 - - : cord-290231-4m9lj0uq 52 21 or or CC cord-290231-4m9lj0uq 52 22 M M NNP cord-290231-4m9lj0uq 52 23 - - HYPH cord-290231-4m9lj0uq 52 24 MVE MVE NNP cord-290231-4m9lj0uq 52 25 virus virus NN cord-290231-4m9lj0uq 52 26 was be VBD cord-290231-4m9lj0uq 52 27 added add VBN cord-290231-4m9lj0uq 52 28 , , , cord-290231-4m9lj0uq 52 29 and and CC cord-290231-4m9lj0uq 52 30 plates plate NNS cord-290231-4m9lj0uq 52 31 were be VBD cord-290231-4m9lj0uq 52 32 incubated incubate VBN cord-290231-4m9lj0uq 52 33 for for IN cord-290231-4m9lj0uq 52 34 1 1 CD cord-290231-4m9lj0uq 52 35 hr hr NN cord-290231-4m9lj0uq 52 36 at at IN cord-290231-4m9lj0uq 52 37 37 37 CD cord-290231-4m9lj0uq 52 38 " " '' cord-290231-4m9lj0uq 52 39 . . . cord-290231-4m9lj0uq 53 1 Ascites Ascites NNPS cord-290231-4m9lj0uq 53 2 of of IN cord-290231-4m9lj0uq 53 3 the the DT cord-290231-4m9lj0uq 53 4 various various JJ cord-290231-4m9lj0uq 53 5 MAbs MAbs NNP cord-290231-4m9lj0uq 53 6 were be VBD cord-290231-4m9lj0uq 53 7 then then RB cord-290231-4m9lj0uq 53 8 titrated titrate VBN cord-290231-4m9lj0uq 53 9 on on IN cord-290231-4m9lj0uq 53 10 both both DT cord-290231-4m9lj0uq 53 11 viruses virus NNS cord-290231-4m9lj0uq 53 12 for for IN cord-290231-4m9lj0uq 53 13 1 1 CD cord-290231-4m9lj0uq 53 14 hr hr NN cord-290231-4m9lj0uq 53 15 at at IN cord-290231-4m9lj0uq 53 16 37 37 CD cord-290231-4m9lj0uq 53 17 " " `` cord-290231-4m9lj0uq 53 18 starting start VBG cord-290231-4m9lj0uq 53 19 at at IN cord-290231-4m9lj0uq 53 20 1 1 CD cord-290231-4m9lj0uq 53 21 : : SYM cord-290231-4m9lj0uq 53 22 100 100 CD cord-290231-4m9lj0uq 53 23 or or CC cord-290231-4m9lj0uq 53 24 1 1 CD cord-290231-4m9lj0uq 53 25 : : SYM cord-290231-4m9lj0uq 53 26 1000 1000 CD cord-290231-4m9lj0uq 53 27 dilution dilution NN cord-290231-4m9lj0uq 53 28 depending depend VBG cord-290231-4m9lj0uq 53 29 on on IN cord-290231-4m9lj0uq 53 30 antibody antibody NN cord-290231-4m9lj0uq 53 31 potency potency NN cord-290231-4m9lj0uq 53 32 . . . cord-290231-4m9lj0uq 54 1 Bounds Bounds NNP cord-290231-4m9lj0uq 54 2 MAbs MAbs NNP cord-290231-4m9lj0uq 54 3 were be VBD cord-290231-4m9lj0uq 54 4 detected detect VBN cord-290231-4m9lj0uq 54 5 by by IN cord-290231-4m9lj0uq 54 6 goat goat NN cord-290231-4m9lj0uq 54 7 anti anti JJ cord-290231-4m9lj0uq 54 8 - - JJ cord-290231-4m9lj0uq 54 9 mouse mouse JJ cord-290231-4m9lj0uq 54 10 alkaline alkaline JJ cord-290231-4m9lj0uq 54 11 phosphatase phosphatase NN cord-290231-4m9lj0uq 54 12 conjugate conjugate NN cord-290231-4m9lj0uq 54 13 . . . cord-290231-4m9lj0uq 55 1 Quantitation quantitation NN cord-290231-4m9lj0uq 55 2 of of IN cord-290231-4m9lj0uq 55 3 bound bind VBN cord-290231-4m9lj0uq 55 4 conjugate conjugate NN cord-290231-4m9lj0uq 55 5 was be VBD cord-290231-4m9lj0uq 55 6 determined determine VBN cord-290231-4m9lj0uq 55 7 by by IN cord-290231-4m9lj0uq 55 8 adding add VBG cord-290231-4m9lj0uq 55 9 Sigma Sigma NNP cord-290231-4m9lj0uq 55 10 104 104 CD cord-290231-4m9lj0uq 55 11 substrate substrate NN cord-290231-4m9lj0uq 55 12 and and CC cord-290231-4m9lj0uq 55 13 reading read VBG cord-290231-4m9lj0uq 55 14 absorbance absorbance NN cord-290231-4m9lj0uq 55 15 at at IN cord-290231-4m9lj0uq 55 16 405 405 CD cord-290231-4m9lj0uq 55 17 nm nm NN cord-290231-4m9lj0uq 55 18 in in IN cord-290231-4m9lj0uq 55 19 a a DT cord-290231-4m9lj0uq 55 20 Titertek Titertek NNP cord-290231-4m9lj0uq 55 21 MC MC NNP cord-290231-4m9lj0uq 55 22 spectrophotometer spectrophotometer NN cord-290231-4m9lj0uq 55 23 after after IN cord-290231-4m9lj0uq 55 24 30 30 CD cord-290231-4m9lj0uq 55 25 min min NN cord-290231-4m9lj0uq 55 26 . . . cord-290231-4m9lj0uq 56 1 The the DT cord-290231-4m9lj0uq 56 2 specificity specificity NN cord-290231-4m9lj0uq 56 3 and and CC cord-290231-4m9lj0uq 56 4 the the DT cord-290231-4m9lj0uq 56 5 biological biological JJ cord-290231-4m9lj0uq 56 6 activity activity NN cord-290231-4m9lj0uq 56 7 of of IN cord-290231-4m9lj0uq 56 8 all all DT cord-290231-4m9lj0uq 56 9 MAbs MAbs NNP cord-290231-4m9lj0uq 56 10 except except IN cord-290231-4m9lj0uq 56 11 6B4A-10 6B4A-10 NNP cord-290231-4m9lj0uq 56 12 have have VBP cord-290231-4m9lj0uq 56 13 been be VBN cord-290231-4m9lj0uq 56 14 published publish VBN cord-290231-4m9lj0uq 56 15 elsewhere elsewhere RB cord-290231-4m9lj0uq 57 1 ( ( -LRB- cord-290231-4m9lj0uq 57 2 Hawkes Hawkes NNP cord-290231-4m9lj0uq 57 3 et et NNP cord-290231-4m9lj0uq 57 4 a/. a/. NNP cord-290231-4m9lj0uq 57 5 , , , cord-290231-4m9lj0uq 57 6 1988 1988 CD cord-290231-4m9lj0uq 57 7 ) ) -RRB- cord-290231-4m9lj0uq 57 8 . . . cord-290231-4m9lj0uq 58 1 MAb MAb NNS cord-290231-4m9lj0uq 58 2 6B4A-10 6B4A-10 NNP cord-290231-4m9lj0uq 58 3 was be VBD cord-290231-4m9lj0uq 58 4 prepared prepare VBN cord-290231-4m9lj0uq 58 5 against against IN cord-290231-4m9lj0uq 58 6 Japanese japanese JJ cord-290231-4m9lj0uq 58 7 encephalitis encephalitis JJ cord-290231-4m9lj0uq 58 8 virus virus NN cord-290231-4m9lj0uq 58 9 , , , cord-290231-4m9lj0uq 58 10 strain strain VB cord-290231-4m9lj0uq 58 11 Nakayama Nakayama NNP cord-290231-4m9lj0uq 58 12 , , , cord-290231-4m9lj0uq 58 13 using use VBG cord-290231-4m9lj0uq 58 14 standard standard JJ cord-290231-4m9lj0uq 58 15 hybridoma hybridoma NN cord-290231-4m9lj0uq 58 16 techniques technique NNS cord-290231-4m9lj0uq 58 17 . . . cord-290231-4m9lj0uq 59 1 This this DT cord-290231-4m9lj0uq 59 2 MAb MAb NNS cord-290231-4m9lj0uq 59 3 reacted react VBD cord-290231-4m9lj0uq 59 4 with with IN cord-290231-4m9lj0uq 59 5 all all DT cord-290231-4m9lj0uq 59 6 members member NNS cord-290231-4m9lj0uq 59 7 of of IN cord-290231-4m9lj0uq 59 8 the the DT cord-290231-4m9lj0uq 59 9 MVE MVE NNP cord-290231-4m9lj0uq 59 10 virus virus NN cord-290231-4m9lj0uq 59 11 serocomplex serocomplex NNP cord-290231-4m9lj0uq 59 12 , , , cord-290231-4m9lj0uq 59 13 blocked block VBD cord-290231-4m9lj0uq 59 14 MVE MVE NNP cord-290231-4m9lj0uq 59 15 virus virus NN cord-290231-4m9lj0uq 59 16 - - HYPH cord-290231-4m9lj0uq 59 17 mediated mediate VBN cord-290231-4m9lj0uq 59 18 hemagglutination hemagglutination NN cord-290231-4m9lj0uq 59 19 , , , cord-290231-4m9lj0uq 59 20 and and CC cord-290231-4m9lj0uq 59 21 neutralized neutralize VBD cord-290231-4m9lj0uq 59 22 MVE MVE NNP cord-290231-4m9lj0uq 59 23 virus virus NN cord-290231-4m9lj0uq 59 24 infectivity infectivity NN cord-290231-4m9lj0uq 59 25 in in IN cord-290231-4m9lj0uq 59 26 vitro vitro FW cord-290231-4m9lj0uq 59 27 . . . cord-290231-4m9lj0uq 60 1 The the DT cord-290231-4m9lj0uq 60 2 E e NN cord-290231-4m9lj0uq 60 3 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 60 4 epitope epitope NN cord-290231-4m9lj0uq 60 5 defined define VBN cord-290231-4m9lj0uq 60 6 by by IN cord-290231-4m9lj0uq 60 7 6B4A-10 6B4A-10 NNP cord-290231-4m9lj0uq 60 8 has have VBZ cord-290231-4m9lj0uq 60 9 been be VBN cord-290231-4m9lj0uq 60 10 designated designate VBN cord-290231-4m9lj0uq 60 11 E-8 E-8 NNP cord-290231-4m9lj0uq 60 12 . . . cord-290231-4m9lj0uq 61 1 The the DT cord-290231-4m9lj0uq 61 2 acid acid JJ cord-290231-4m9lj0uq 61 3 sensitivity sensitivity NN cord-290231-4m9lj0uq 61 4 of of IN cord-290231-4m9lj0uq 61 5 the the DT cord-290231-4m9lj0uq 61 6 MVE MVE NNP cord-290231-4m9lj0uq 61 7 virus virus NN cord-290231-4m9lj0uq 61 8 epitopes epitope NNS cord-290231-4m9lj0uq 61 9 was be VBD cord-290231-4m9lj0uq 61 10 determined determine VBN cord-290231-4m9lj0uq 61 11 using use VBG cord-290231-4m9lj0uq 61 12 a a DT cord-290231-4m9lj0uq 61 13 modification modification NN cord-290231-4m9lj0uq 61 14 of of IN cord-290231-4m9lj0uq 61 15 the the DT cord-290231-4m9lj0uq 61 16 standard standard JJ cord-290231-4m9lj0uq 61 17 ELISA ELISA NNP cord-290231-4m9lj0uq 61 18 protocol protocol NN cord-290231-4m9lj0uq 61 19 . . . cord-290231-4m9lj0uq 62 1 Two two CD cord-290231-4m9lj0uq 62 2 hundred hundred CD cord-290231-4m9lj0uq 62 3 and and CC cord-290231-4m9lj0uq 62 4 fifty fifty CD cord-290231-4m9lj0uq 62 5 microliters microliter NNS cord-290231-4m9lj0uq 62 6 of of IN cord-290231-4m9lj0uq 62 7 purified purify VBN cord-290231-4m9lj0uq 62 8 MVE MVE NNP cord-290231-4m9lj0uq 62 9 virus virus NN cord-290231-4m9lj0uq 62 10 ( ( -LRB- cord-290231-4m9lj0uq 62 11 100 100 CD cord-290231-4m9lj0uq 62 12 pg pg NN cord-290231-4m9lj0uq 62 13 total total NN cord-290231-4m9lj0uq 62 14 protein protein NN cord-290231-4m9lj0uq 62 15 ) ) -RRB- cord-290231-4m9lj0uq 62 16 in in IN cord-290231-4m9lj0uq 62 17 0.2 0.2 CD cord-290231-4m9lj0uq 62 18 M M NNP cord-290231-4m9lj0uq 62 19 Tris Tris NNP cord-290231-4m9lj0uq 62 20 - - HYPH cord-290231-4m9lj0uq 62 21 HCI HCI NNP cord-290231-4m9lj0uq 62 22 , , , cord-290231-4m9lj0uq 62 23 pH pH NNP cord-290231-4m9lj0uq 62 24 8.0 8.0 CD cord-290231-4m9lj0uq 62 25 , , , cord-290231-4m9lj0uq 62 26 was be VBD cord-290231-4m9lj0uq 62 27 adjusted adjust VBN cord-290231-4m9lj0uq 62 28 to to IN cord-290231-4m9lj0uq 62 29 pH pH NNP cord-290231-4m9lj0uq 62 30 6.0 6.0 CD cord-290231-4m9lj0uq 62 31 using use VBG cord-290231-4m9lj0uq 62 32 120 120 CD cord-290231-4m9lj0uq 62 33 ~1 ~1 CD cord-290231-4m9lj0uq 62 34 of of IN cord-290231-4m9lj0uq 62 35 0.2 0.2 CD cord-290231-4m9lj0uq 62 36 M M NNP cord-290231-4m9lj0uq 62 37 HCI HCI NNP cord-290231-4m9lj0uq 62 38 and and CC cord-290231-4m9lj0uq 62 39 incubated incubate VBD cord-290231-4m9lj0uq 62 40 at at IN cord-290231-4m9lj0uq 62 41 25 25 CD cord-290231-4m9lj0uq 62 42 " " `` cord-290231-4m9lj0uq 62 43 for for IN cord-290231-4m9lj0uq 62 44 30 30 CD cord-290231-4m9lj0uq 62 45 min min NN cord-290231-4m9lj0uq 62 46 . . . cord-290231-4m9lj0uq 63 1 Both both DT cord-290231-4m9lj0uq 63 2 acid acid NN cord-290231-4m9lj0uq 63 3 - - HYPH cord-290231-4m9lj0uq 63 4 treated treat VBN cord-290231-4m9lj0uq 63 5 virus virus NN cord-290231-4m9lj0uq 63 6 and and CC cord-290231-4m9lj0uq 63 7 untreated untreated JJ cord-290231-4m9lj0uq 63 8 virus virus NN cord-290231-4m9lj0uq 63 9 ( ( -LRB- cord-290231-4m9lj0uq 63 10 pH pH NNP cord-290231-4m9lj0uq 63 11 8.0 8.0 CD cord-290231-4m9lj0uq 63 12 ) ) -RRB- cord-290231-4m9lj0uq 63 13 samples sample NNS cord-290231-4m9lj0uq 63 14 were be VBD cord-290231-4m9lj0uq 63 15 diluted dilute VBN cord-290231-4m9lj0uq 63 16 to to IN cord-290231-4m9lj0uq 63 17 5 5 CD cord-290231-4m9lj0uq 63 18 ml ml NNS cord-290231-4m9lj0uq 63 19 with with IN cord-290231-4m9lj0uq 63 20 carbonate carbonate NN cord-290231-4m9lj0uq 63 21 buffer buffer NN cord-290231-4m9lj0uq 63 22 , , , cord-290231-4m9lj0uq 63 23 pH pH NNP cord-290231-4m9lj0uq 63 24 9.5 9.5 CD cord-290231-4m9lj0uq 63 25 . . . cord-290231-4m9lj0uq 64 1 lmmulon lmmulon NNP cord-290231-4m9lj0uq 64 2 II II NNP cord-290231-4m9lj0uq 64 3 microtiter microtiter NN cord-290231-4m9lj0uq 64 4 plates plate NNS cord-290231-4m9lj0uq 64 5 were be VBD cord-290231-4m9lj0uq 64 6 coated coat VBN cord-290231-4m9lj0uq 64 7 with with IN cord-290231-4m9lj0uq 64 8 100 100 CD cord-290231-4m9lj0uq 64 9 ~1 ~1 CD cord-290231-4m9lj0uq 64 10 virus virus NN cord-290231-4m9lj0uq 64 11 per per IN cord-290231-4m9lj0uq 64 12 well well RB cord-290231-4m9lj0uq 64 13 and and CC cord-290231-4m9lj0uq 64 14 incubated incubate VBN cord-290231-4m9lj0uq 64 15 overnight overnight RB cord-290231-4m9lj0uq 64 16 at at IN cord-290231-4m9lj0uq 64 17 4 4 CD cord-290231-4m9lj0uq 64 18 " " '' cord-290231-4m9lj0uq 64 19 . . . cord-290231-4m9lj0uq 65 1 The the DT cord-290231-4m9lj0uq 65 2 plates plate NNS cord-290231-4m9lj0uq 65 3 were be VBD cord-290231-4m9lj0uq 65 4 blocked block VBN cord-290231-4m9lj0uq 65 5 for for IN cord-290231-4m9lj0uq 65 6 1 1 CD cord-290231-4m9lj0uq 65 7 hr hr NN cord-290231-4m9lj0uq 65 8 at at IN cord-290231-4m9lj0uq 65 9 37 37 CD cord-290231-4m9lj0uq 65 10 " " `` cord-290231-4m9lj0uq 65 11 with with IN cord-290231-4m9lj0uq 65 12 3 3 CD cord-290231-4m9lj0uq 65 13 % % NN cord-290231-4m9lj0uq 65 14 BSA BSA NNP cord-290231-4m9lj0uq 65 15 in in IN cord-290231-4m9lj0uq 65 16 PBS PBS NNP cord-290231-4m9lj0uq 65 17 . . . cord-290231-4m9lj0uq 66 1 Protein Protein NNP cord-290231-4m9lj0uq 66 2 MAbs MAbs NNP cord-290231-4m9lj0uq 66 3 were be VBD cord-290231-4m9lj0uq 66 4 diluted dilute VBN cord-290231-4m9lj0uq 66 5 geometrically geometrically RB cord-290231-4m9lj0uq 66 6 ( ( -LRB- cord-290231-4m9lj0uq 66 7 from from IN cord-290231-4m9lj0uq 66 8 10 10 CD cord-290231-4m9lj0uq 66 9 pg pg NN cord-290231-4m9lj0uq 66 10 / / SYM cord-290231-4m9lj0uq 66 11 well well JJ cord-290231-4m9lj0uq 66 12 total total JJ cord-290231-4m9lj0uq 66 13 protein protein NN cord-290231-4m9lj0uq 66 14 ) ) -RRB- cord-290231-4m9lj0uq 66 15 and and CC cord-290231-4m9lj0uq 66 16 added add VBD cord-290231-4m9lj0uq 66 17 to to IN cord-290231-4m9lj0uq 66 18 each each DT cord-290231-4m9lj0uq 66 19 well well RB cord-290231-4m9lj0uq 66 20 . . . cord-290231-4m9lj0uq 67 1 After after IN cord-290231-4m9lj0uq 67 2 2 2 CD cord-290231-4m9lj0uq 67 3 hr hr NN cord-290231-4m9lj0uq 67 4 at at IN cord-290231-4m9lj0uq 67 5 25 25 CD cord-290231-4m9lj0uq 67 6 " " '' cord-290231-4m9lj0uq 67 7 , , , cord-290231-4m9lj0uq 67 8 the the DT cord-290231-4m9lj0uq 67 9 plates plate NNS cord-290231-4m9lj0uq 67 10 were be VBD cord-290231-4m9lj0uq 67 11 washed wash VBN cord-290231-4m9lj0uq 67 12 and and CC cord-290231-4m9lj0uq 67 13 further further RB cord-290231-4m9lj0uq 67 14 incubated incubate VBN cord-290231-4m9lj0uq 67 15 with with IN cord-290231-4m9lj0uq 67 16 a a DT cord-290231-4m9lj0uq 67 17 1:500 1:500 CD cord-290231-4m9lj0uq 67 18 dilution dilution NN cord-290231-4m9lj0uq 67 19 of of IN cord-290231-4m9lj0uq 67 20 goat goat NN cord-290231-4m9lj0uq 67 21 antimouse antimouse IN cord-290231-4m9lj0uq 67 22 alkaline alkaline JJ cord-290231-4m9lj0uq 67 23 phosphatase phosphatase NN cord-290231-4m9lj0uq 67 24 conjugate conjugate NN cord-290231-4m9lj0uq 67 25 . . . cord-290231-4m9lj0uq 68 1 Bound bound JJ cord-290231-4m9lj0uq 68 2 antibody antibody NN cord-290231-4m9lj0uq 68 3 was be VBD cord-290231-4m9lj0uq 68 4 detected detect VBN cord-290231-4m9lj0uq 68 5 by by IN cord-290231-4m9lj0uq 68 6 addition addition NN cord-290231-4m9lj0uq 68 7 of of IN cord-290231-4m9lj0uq 68 8 Sigma Sigma NNP cord-290231-4m9lj0uq 68 9 104 104 CD cord-290231-4m9lj0uq 68 10 substrate substrate NN cord-290231-4m9lj0uq 68 11 , , , cord-290231-4m9lj0uq 68 12 and and CC cord-290231-4m9lj0uq 68 13 absorbance absorbance NN cord-290231-4m9lj0uq 68 14 was be VBD cord-290231-4m9lj0uq 68 15 measured measure VBN cord-290231-4m9lj0uq 68 16 at at IN cord-290231-4m9lj0uq 68 17 405 405 CD cord-290231-4m9lj0uq 68 18 nm nm NN cord-290231-4m9lj0uq 68 19 after after IN cord-290231-4m9lj0uq 68 20 5 5 CD cord-290231-4m9lj0uq 68 21 min min NN cord-290231-4m9lj0uq 68 22 . . . cord-290231-4m9lj0uq 69 1 Although although IN cord-290231-4m9lj0uq 69 2 each each DT cord-290231-4m9lj0uq 69 3 antibody antibody NN cord-290231-4m9lj0uq 69 4 was be VBD cord-290231-4m9lj0uq 69 5 tested test VBN cord-290231-4m9lj0uq 69 6 in in IN cord-290231-4m9lj0uq 69 7 a a DT cord-290231-4m9lj0uq 69 8 dilution dilution NN cord-290231-4m9lj0uq 69 9 series series NN cord-290231-4m9lj0uq 69 10 , , , cord-290231-4m9lj0uq 69 11 only only RB cord-290231-4m9lj0uq 69 12 the the DT cord-290231-4m9lj0uq 69 13 results result NNS cord-290231-4m9lj0uq 69 14 with with IN cord-290231-4m9lj0uq 69 15 1.25 1.25 CD cord-290231-4m9lj0uq 69 16 gg gg NNP cord-290231-4m9lj0uq 69 17 antibody antibody NN cord-290231-4m9lj0uq 69 18 are be VBP cord-290231-4m9lj0uq 69 19 reported report VBN cord-290231-4m9lj0uq 69 20 . . . cord-290231-4m9lj0uq 70 1 Analysis analysis NN cord-290231-4m9lj0uq 70 2 of of IN cord-290231-4m9lj0uq 70 3 the the DT cord-290231-4m9lj0uq 70 4 MAb MAb NNS cord-290231-4m9lj0uq 70 5 reactivities reactivity NNS cord-290231-4m9lj0uq 70 6 with with IN cord-290231-4m9lj0uq 70 7 reduced reduced JJ cord-290231-4m9lj0uq 70 8 and and CC cord-290231-4m9lj0uq 70 9 nonreduced nonreduced JJ cord-290231-4m9lj0uq 70 10 MVE MVE NNP cord-290231-4m9lj0uq 70 11 virus virus NN cord-290231-4m9lj0uq 70 12 PAGE page NN cord-290231-4m9lj0uq 70 13 sample sample NN cord-290231-4m9lj0uq 70 14 buffer buffer NN cord-290231-4m9lj0uq 70 15 ( ( -LRB- cord-290231-4m9lj0uq 70 16 Laemmli Laemmli NNP cord-290231-4m9lj0uq 70 17 , , , cord-290231-4m9lj0uq 70 18 1970 1970 CD cord-290231-4m9lj0uq 70 19 ) ) -RRB- cord-290231-4m9lj0uq 70 20 with with IN cord-290231-4m9lj0uq 70 21 or or CC cord-290231-4m9lj0uq 70 22 without without IN cord-290231-4m9lj0uq 70 23 the the DT cord-290231-4m9lj0uq 70 24 reducing reduce VBG cord-290231-4m9lj0uq 70 25 agent agent NN cord-290231-4m9lj0uq 70 26 , , , cord-290231-4m9lj0uq 70 27 2-mercaptoethanol 2-mercaptoethanol CD cord-290231-4m9lj0uq 70 28 , , , cord-290231-4m9lj0uq 70 29 was be VBD cord-290231-4m9lj0uq 70 30 added add VBN cord-290231-4m9lj0uq 70 31 to to IN cord-290231-4m9lj0uq 70 32 10 10 CD cord-290231-4m9lj0uq 70 33 pg pg CD cord-290231-4m9lj0uq 70 34 of of IN cord-290231-4m9lj0uq 70 35 purified purify VBN cord-290231-4m9lj0uq 70 36 MVE MVE NNP cord-290231-4m9lj0uq 70 37 virus virus NN cord-290231-4m9lj0uq 70 38 . . . cord-290231-4m9lj0uq 71 1 Samples sample NNS cord-290231-4m9lj0uq 71 2 were be VBD cord-290231-4m9lj0uq 71 3 boiled boil VBN cord-290231-4m9lj0uq 71 4 for for IN cord-290231-4m9lj0uq 71 5 3 3 CD cord-290231-4m9lj0uq 71 6 min min NN cord-290231-4m9lj0uq 71 7 at at IN cord-290231-4m9lj0uq 71 8 100 100 CD cord-290231-4m9lj0uq 71 9 " " '' cord-290231-4m9lj0uq 71 10 and and CC cord-290231-4m9lj0uq 71 11 separated separate VBD cord-290231-4m9lj0uq 71 12 on on IN cord-290231-4m9lj0uq 71 13 12.5 12.5 CD cord-290231-4m9lj0uq 71 14 % % NN cord-290231-4m9lj0uq 71 15 SDS SDS NNP cord-290231-4m9lj0uq 71 16 gels gel NNS cord-290231-4m9lj0uq 71 17 . . . cord-290231-4m9lj0uq 72 1 Proteins protein NNS cord-290231-4m9lj0uq 72 2 were be VBD cord-290231-4m9lj0uq 72 3 then then RB cord-290231-4m9lj0uq 72 4 transferred transfer VBN cord-290231-4m9lj0uq 72 5 to to IN cord-290231-4m9lj0uq 72 6 nitrocellulose nitrocellulose NN cord-290231-4m9lj0uq 72 7 sheet sheet NN cord-290231-4m9lj0uq 72 8 ( ( -LRB- cord-290231-4m9lj0uq 72 9 Towbin Towbin NNP cord-290231-4m9lj0uq 72 10 et et NNP cord-290231-4m9lj0uq 72 11 a/. a/. NN cord-290231-4m9lj0uq 72 12 , , , cord-290231-4m9lj0uq 72 13 1979 1979 CD cord-290231-4m9lj0uq 72 14 ) ) -RRB- cord-290231-4m9lj0uq 72 15 and and CC cord-290231-4m9lj0uq 72 16 blocked block VBD cord-290231-4m9lj0uq 72 17 with with IN cord-290231-4m9lj0uq 72 18 3 3 CD cord-290231-4m9lj0uq 72 19 % % NN cord-290231-4m9lj0uq 72 20 goat goat NN cord-290231-4m9lj0uq 72 21 serum serum NN cord-290231-4m9lj0uq 72 22 . . . cord-290231-4m9lj0uq 73 1 The the DT cord-290231-4m9lj0uq 73 2 reactivity reactivity NN cord-290231-4m9lj0uq 73 3 of of IN cord-290231-4m9lj0uq 73 4 MAbs MAbs NNP cord-290231-4m9lj0uq 73 5 defining define VBG cord-290231-4m9lj0uq 73 6 the the DT cord-290231-4m9lj0uq 73 7 epitopes epitope NNS cord-290231-4m9lj0uq 73 8 E-4b e-4b NN cord-290231-4m9lj0uq 73 9 , , , cord-290231-4m9lj0uq 73 10 E-6 E-6 NNP cord-290231-4m9lj0uq 73 11 , , , cord-290231-4m9lj0uq 73 12 or or CC cord-290231-4m9lj0uq 73 13 E-8 E-8 NNP cord-290231-4m9lj0uq 73 14 with with IN cord-290231-4m9lj0uq 73 15 reduced reduced JJ cord-290231-4m9lj0uq 73 16 or or CC cord-290231-4m9lj0uq 73 17 nonreduced nonreduced JJ cord-290231-4m9lj0uq 73 18 forms form NNS cord-290231-4m9lj0uq 73 19 of of IN cord-290231-4m9lj0uq 73 20 MVE MVE NNP cord-290231-4m9lj0uq 73 21 virus virus NN cord-290231-4m9lj0uq 73 22 E e NN cord-290231-4m9lj0uq 73 23 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 73 24 was be VBD cord-290231-4m9lj0uq 73 25 assessed assess VBN cord-290231-4m9lj0uq 73 26 using use VBG cord-290231-4m9lj0uq 73 27 goat goat NN cord-290231-4m9lj0uq 73 28 anti anti JJ cord-290231-4m9lj0uq 73 29 - - JJ cord-290231-4m9lj0uq 73 30 mouse mouse JJ cord-290231-4m9lj0uq 73 31 alkaline alkaline JJ cord-290231-4m9lj0uq 73 32 phosphatase phosphatase NN cord-290231-4m9lj0uq 73 33 conjugate conjugate NN cord-290231-4m9lj0uq 73 34 ( ( -LRB- cord-290231-4m9lj0uq 73 35 Jackson Jackson NNP cord-290231-4m9lj0uq 73 36 lmmuno lmmuno NNP cord-290231-4m9lj0uq 73 37 Research Research NNP cord-290231-4m9lj0uq 73 38 , , , cord-290231-4m9lj0uq 73 39 West West NNP cord-290231-4m9lj0uq 73 40 Grove Grove NNP cord-290231-4m9lj0uq 73 41 , , , cord-290231-4m9lj0uq 73 42 PA PA NNP cord-290231-4m9lj0uq 73 43 ) ) -RRB- cord-290231-4m9lj0uq 73 44 . . . cord-290231-4m9lj0uq 74 1 Proteolytic proteolytic JJ cord-290231-4m9lj0uq 74 2 digestion digestion NN cord-290231-4m9lj0uq 74 3 of of IN cord-290231-4m9lj0uq 74 4 the the DT cord-290231-4m9lj0uq 74 5 native native JJ cord-290231-4m9lj0uq 74 6 MVE MVE NNP cord-290231-4m9lj0uq 74 7 virus virus NN cord-290231-4m9lj0uq 74 8 was be VBD cord-290231-4m9lj0uq 74 9 performed perform VBN cord-290231-4m9lj0uq 74 10 in in IN cord-290231-4m9lj0uq 74 11 D D NNP cord-290231-4m9lj0uq 74 12 - - HYPH cord-290231-4m9lj0uq 74 13 MEM mem NN cord-290231-4m9lj0uq 74 14 , , , cord-290231-4m9lj0uq 74 15 pH pH NNP cord-290231-4m9lj0uq 74 16 7.4 7.4 CD cord-290231-4m9lj0uq 74 17 , , , cord-290231-4m9lj0uq 74 18 using use VBG cord-290231-4m9lj0uq 74 19 predetermined predetermine VBN cord-290231-4m9lj0uq 74 20 concentrations concentration NNS cord-290231-4m9lj0uq 74 21 of of IN cord-290231-4m9lj0uq 74 22 either either DT cord-290231-4m9lj0uq 74 23 trypsin trypsin NN cord-290231-4m9lj0uq 74 24 or or CC cord-290231-4m9lj0uq 74 25 V8 v8 NN cord-290231-4m9lj0uq 74 26 protease protease NN cord-290231-4m9lj0uq 74 27 ( ( -LRB- cord-290231-4m9lj0uq 74 28 Sigma Sigma NNP cord-290231-4m9lj0uq 74 29 Chemical Chemical NNP cord-290231-4m9lj0uq 74 30 Co. Co. NNP cord-290231-4m9lj0uq 74 31 ) ) -RRB- cord-290231-4m9lj0uq 74 32 and and CC cord-290231-4m9lj0uq 74 33 the the DT cord-290231-4m9lj0uq 74 34 fragments fragment NNS cord-290231-4m9lj0uq 74 35 reacting react VBG cord-290231-4m9lj0uq 74 36 with with IN cord-290231-4m9lj0uq 74 37 MAb MAb NNPS cord-290231-4m9lj0uq 74 38 E-8 E-8 NNP cord-290231-4m9lj0uq 74 39 were be VBD cord-290231-4m9lj0uq 74 40 identified identify VBN cord-290231-4m9lj0uq 74 41 by by IN cord-290231-4m9lj0uq 74 42 using use VBG cord-290231-4m9lj0uq 74 43 goat goat NN cord-290231-4m9lj0uq 74 44 anti anti JJ cord-290231-4m9lj0uq 74 45 - - JJ cord-290231-4m9lj0uq 74 46 mouse mouse JJ cord-290231-4m9lj0uq 74 47 alkaline alkaline JJ cord-290231-4m9lj0uq 74 48 phosphatase phosphatase NN cord-290231-4m9lj0uq 74 49 conjugate conjugate NN cord-290231-4m9lj0uq 74 50 ( ( -LRB- cord-290231-4m9lj0uq 74 51 see see VB cord-290231-4m9lj0uq 74 52 below below RB cord-290231-4m9lj0uq 74 53 ) ) -RRB- cord-290231-4m9lj0uq 74 54 . . . cord-290231-4m9lj0uq 75 1 For for IN cord-290231-4m9lj0uq 75 2 the the DT cord-290231-4m9lj0uq 75 3 proteolytic proteolytic JJ cord-290231-4m9lj0uq 75 4 digestion digestion NN cord-290231-4m9lj0uq 75 5 of of IN cord-290231-4m9lj0uq 75 6 the the DT cord-290231-4m9lj0uq 75 7 predenatured predenatured JJ cord-290231-4m9lj0uq 75 8 virus virus NN cord-290231-4m9lj0uq 75 9 , , , cord-290231-4m9lj0uq 75 10 60 60 CD cord-290231-4m9lj0uq 75 11 pg pg NN cord-290231-4m9lj0uq 75 12 of of IN cord-290231-4m9lj0uq 75 13 purified purify VBN cord-290231-4m9lj0uq 75 14 MVE MVE NNP cord-290231-4m9lj0uq 75 15 virus virus NN cord-290231-4m9lj0uq 75 16 in in IN cord-290231-4m9lj0uq 75 17 D D NNP cord-290231-4m9lj0uq 75 18 - - HYPH cord-290231-4m9lj0uq 75 19 MEM MEM NNP cord-290231-4m9lj0uq 75 20 was be VBD cord-290231-4m9lj0uq 75 21 precipitated precipitate VBN cord-290231-4m9lj0uq 75 22 in in IN cord-290231-4m9lj0uq 75 23 60 60 CD cord-290231-4m9lj0uq 75 24 % % NN cord-290231-4m9lj0uq 75 25 TCA TCA NNP cord-290231-4m9lj0uq 75 26 ( ( -LRB- cord-290231-4m9lj0uq 75 27 end end NN cord-290231-4m9lj0uq 75 28 concentration concentration NN cord-290231-4m9lj0uq 75 29 12 12 CD cord-290231-4m9lj0uq 75 30 % % NN cord-290231-4m9lj0uq 75 31 ) ) -RRB- cord-290231-4m9lj0uq 75 32 on on IN cord-290231-4m9lj0uq 75 33 an an DT cord-290231-4m9lj0uq 75 34 ice ice NN cord-290231-4m9lj0uq 75 35 bath bath NN cord-290231-4m9lj0uq 75 36 for for IN cord-290231-4m9lj0uq 75 37 30 30 CD cord-290231-4m9lj0uq 75 38 min min NN cord-290231-4m9lj0uq 75 39 , , , cord-290231-4m9lj0uq 75 40 washed wash VBN cord-290231-4m9lj0uq 75 41 with with IN cord-290231-4m9lj0uq 75 42 cold cold JJ cord-290231-4m9lj0uq 75 43 acetone acetone NN cord-290231-4m9lj0uq 75 44 , , , cord-290231-4m9lj0uq 75 45 and and CC cord-290231-4m9lj0uq 75 46 resuspended resuspend VBD cord-290231-4m9lj0uq 75 47 in in IN cord-290231-4m9lj0uq 75 48 50 50 CD cord-290231-4m9lj0uq 75 49 ~1 ~1 NNP cord-290231-4m9lj0uq 75 50 0.125 0.125 CD cord-290231-4m9lj0uq 75 51 M M NNP cord-290231-4m9lj0uq 75 52 Tris Tris NNP cord-290231-4m9lj0uq 75 53 , , , cord-290231-4m9lj0uq 75 54 pH pH NNP cord-290231-4m9lj0uq 75 55 6.8 6.8 CD cord-290231-4m9lj0uq 75 56 , , , cord-290231-4m9lj0uq 75 57 containing contain VBG cord-290231-4m9lj0uq 75 58 0.5 0.5 CD cord-290231-4m9lj0uq 75 59 % % NN cord-290231-4m9lj0uq 75 60 SDS sds NN cord-290231-4m9lj0uq 75 61 and and CC cord-290231-4m9lj0uq 75 62 10 10 CD cord-290231-4m9lj0uq 75 63 % % NN cord-290231-4m9lj0uq 75 64 glycerol glycerol NN cord-290231-4m9lj0uq 75 65 . . . cord-290231-4m9lj0uq 76 1 After after IN cord-290231-4m9lj0uq 76 2 24 24 CD cord-290231-4m9lj0uq 76 3 hr hr NN cord-290231-4m9lj0uq 76 4 incubation incubation NN cord-290231-4m9lj0uq 76 5 at at IN cord-290231-4m9lj0uq 76 6 37 37 CD cord-290231-4m9lj0uq 76 7 " " `` cord-290231-4m9lj0uq 76 8 samples sample NNS cord-290231-4m9lj0uq 76 9 were be VBD cord-290231-4m9lj0uq 76 10 boiled boil VBN cord-290231-4m9lj0uq 76 11 for for IN cord-290231-4m9lj0uq 76 12 3 3 CD cord-290231-4m9lj0uq 76 13 min min NN cord-290231-4m9lj0uq 76 14 and and CC cord-290231-4m9lj0uq 76 15 subjected subject VBN cord-290231-4m9lj0uq 76 16 to to IN cord-290231-4m9lj0uq 76 17 proteolytic proteolytic JJ cord-290231-4m9lj0uq 76 18 digestion digestion NN cord-290231-4m9lj0uq 76 19 using use VBG cord-290231-4m9lj0uq 76 20 either either CC cord-290231-4m9lj0uq 76 21 chymotrypsin chymotrypsin NNP cord-290231-4m9lj0uq 76 22 or or CC cord-290231-4m9lj0uq 76 23 V-8 V-8 NNP cord-290231-4m9lj0uq 76 24 proteases protease NNS cord-290231-4m9lj0uq 76 25 ( ( -LRB- cord-290231-4m9lj0uq 76 26 Sigma Sigma NNP cord-290231-4m9lj0uq 76 27 Chemical Chemical NNP cord-290231-4m9lj0uq 76 28 Co. Co. NNP cord-290231-4m9lj0uq 76 29 ) ) -RRB- cord-290231-4m9lj0uq 76 30 at at IN cord-290231-4m9lj0uq 76 31 the the DT cord-290231-4m9lj0uq 76 32 protein protein NN cord-290231-4m9lj0uq 76 33 to to IN cord-290231-4m9lj0uq 76 34 enzyme enzyme NN cord-290231-4m9lj0uq 76 35 ratios ratio NNS cord-290231-4m9lj0uq 76 36 of of IN cord-290231-4m9lj0uq 76 37 4 4 CD cord-290231-4m9lj0uq 76 38 : : : cord-290231-4m9lj0uq 77 1 l l NNP cord-290231-4m9lj0uq 77 2 and and CC cord-290231-4m9lj0uq 77 3 30 30 CD cord-290231-4m9lj0uq 77 4 : : SYM cord-290231-4m9lj0uq 77 5 1 1 CD cord-290231-4m9lj0uq 77 6 , , , cord-290231-4m9lj0uq 77 7 respectively respectively RB cord-290231-4m9lj0uq 77 8 , , , cord-290231-4m9lj0uq 77 9 at at IN cord-290231-4m9lj0uq 77 10 37 37 CD cord-290231-4m9lj0uq 77 11 " " `` cord-290231-4m9lj0uq 77 12 for for IN cord-290231-4m9lj0uq 77 13 30 30 CD cord-290231-4m9lj0uq 77 14 min min NN cord-290231-4m9lj0uq 77 15 . . . cord-290231-4m9lj0uq 78 1 After after IN cord-290231-4m9lj0uq 78 2 addition addition NN cord-290231-4m9lj0uq 78 3 of of IN cord-290231-4m9lj0uq 78 4 SDS SDS NNP cord-290231-4m9lj0uq 78 5 and and CC cord-290231-4m9lj0uq 78 6 2-mercaptoethanol 2-mercaptoethanol CD cord-290231-4m9lj0uq 78 7 ( ( -LRB- cord-290231-4m9lj0uq 78 8 final final JJ cord-290231-4m9lj0uq 78 9 concentrations concentration NNS cord-290231-4m9lj0uq 78 10 4 4 CD cord-290231-4m9lj0uq 78 11 % % NN cord-290231-4m9lj0uq 78 12 ) ) -RRB- cord-290231-4m9lj0uq 79 1 the the DT cord-290231-4m9lj0uq 79 2 samples sample NNS cord-290231-4m9lj0uq 79 3 were be VBD cord-290231-4m9lj0uq 79 4 boiled boil VBN cord-290231-4m9lj0uq 79 5 and and CC cord-290231-4m9lj0uq 79 6 separated separate VBN cord-290231-4m9lj0uq 79 7 on on IN cord-290231-4m9lj0uq 79 8 12.5 12.5 CD cord-290231-4m9lj0uq 79 9 % % NN cord-290231-4m9lj0uq 79 10 SDS SDS NNP cord-290231-4m9lj0uq 79 11 gels gel NNS cord-290231-4m9lj0uq 79 12 . . . cord-290231-4m9lj0uq 80 1 Fragments fragment NNS cord-290231-4m9lj0uq 80 2 were be VBD cord-290231-4m9lj0uq 80 3 transferred transfer VBN cord-290231-4m9lj0uq 80 4 to to IN cord-290231-4m9lj0uq 80 5 nitrocellulose nitrocellulose NN cord-290231-4m9lj0uq 80 6 membrane membrane NN cord-290231-4m9lj0uq 80 7 by by IN cord-290231-4m9lj0uq 80 8 electroblotting electroblotting NNP cord-290231-4m9lj0uq 81 1 ( ( -LRB- cord-290231-4m9lj0uq 81 2 Towbin Towbin NNP cord-290231-4m9lj0uq 81 3 et et NNP cord-290231-4m9lj0uq 81 4 al al NNP cord-290231-4m9lj0uq 81 5 . . NNP cord-290231-4m9lj0uq 81 6 , , , cord-290231-4m9lj0uq 81 7 1979 1979 CD cord-290231-4m9lj0uq 81 8 ) ) -RRB- cord-290231-4m9lj0uq 81 9 . . . cord-290231-4m9lj0uq 82 1 Nitrocellulose nitrocellulose NN cord-290231-4m9lj0uq 82 2 was be VBD cord-290231-4m9lj0uq 82 3 then then RB cord-290231-4m9lj0uq 82 4 blocked block VBN cord-290231-4m9lj0uq 82 5 by by IN cord-290231-4m9lj0uq 82 6 3 3 CD cord-290231-4m9lj0uq 82 7 % % NN cord-290231-4m9lj0uq 82 8 goat goat NN cord-290231-4m9lj0uq 82 9 serum serum NN cord-290231-4m9lj0uq 82 10 in in IN cord-290231-4m9lj0uq 82 11 PBS PBS NNP cord-290231-4m9lj0uq 82 12 , , , cord-290231-4m9lj0uq 82 13 pH pH NNP cord-290231-4m9lj0uq 82 14 7.4 7.4 CD cord-290231-4m9lj0uq 82 15 , , , cord-290231-4m9lj0uq 82 16 for for IN cord-290231-4m9lj0uq 82 17 1 1 CD cord-290231-4m9lj0uq 82 18 hr hr NN cord-290231-4m9lj0uq 82 19 . . . cord-290231-4m9lj0uq 83 1 Strips strip NNS cord-290231-4m9lj0uq 83 2 were be VBD cord-290231-4m9lj0uq 83 3 cut cut VBN cord-290231-4m9lj0uq 83 4 and and CC cord-290231-4m9lj0uq 83 5 incubated incubate VBN cord-290231-4m9lj0uq 83 6 with with IN cord-290231-4m9lj0uq 83 7 either either CC cord-290231-4m9lj0uq 83 8 MAb MAb NNS cord-290231-4m9lj0uq 83 9 ( ( -LRB- cord-290231-4m9lj0uq 83 10 defining define VBG cord-290231-4m9lj0uq 83 11 epitope epitope NN cord-290231-4m9lj0uq 83 12 E-8 E-8 NNP cord-290231-4m9lj0uq 83 13 ) ) -RRB- cord-290231-4m9lj0uq 83 14 or or CC cord-290231-4m9lj0uq 83 15 mouse mouse NNP cord-290231-4m9lj0uq 83 16 sera sera NNP cord-290231-4m9lj0uq 83 17 which which WDT cord-290231-4m9lj0uq 83 18 were be VBD cord-290231-4m9lj0uq 83 19 immunized immunize VBN cord-290231-4m9lj0uq 83 20 with with IN cord-290231-4m9lj0uq 83 21 E e NN cord-290231-4m9lj0uq 83 22 - - JJ cord-290231-4m9lj0uq 83 23 specific specific JJ cord-290231-4m9lj0uq 83 24 synthetic synthetic JJ cord-290231-4m9lj0uq 83 25 peptides peptide NNS cord-290231-4m9lj0uq 83 26 as as IN cord-290231-4m9lj0uq 83 27 follows follow VBZ cord-290231-4m9lj0uq 83 28 : : : cord-290231-4m9lj0uq 83 29 MVE MVE NNP cord-290231-4m9lj0uq 83 30 02 02 CD cord-290231-4m9lj0uq 84 1 , , , cord-290231-4m9lj0uq 84 2 amino amino JJ cord-290231-4m9lj0uq 84 3 acids acid NNS cord-290231-4m9lj0uq 84 4 35 35 CD cord-290231-4m9lj0uq 84 5 - - SYM cord-290231-4m9lj0uq 84 6 50 50 CD cord-290231-4m9lj0uq 85 1 ; ; : cord-290231-4m9lj0uq 85 2 MVE MVE NNP cord-290231-4m9lj0uq 85 3 04 04 CD cord-290231-4m9lj0uq 85 4 , , , cord-290231-4m9lj0uq 85 5 amino amino JJ cord-290231-4m9lj0uq 85 6 acids acid NNS cord-290231-4m9lj0uq 85 7 122-l 122-l CD cord-290231-4m9lj0uq 85 8 41 41 CD cord-290231-4m9lj0uq 85 9 ; ; : cord-290231-4m9lj0uq 85 10 MVE MVE NNP cord-290231-4m9lj0uq 85 11 06 06 CD cord-290231-4m9lj0uq 85 12 , , , cord-290231-4m9lj0uq 85 13 amino amino JJ cord-290231-4m9lj0uq 85 14 acids acid NNS cord-290231-4m9lj0uq 86 1 230 230 CD cord-290231-4m9lj0uq 86 2 - - SYM cord-290231-4m9lj0uq 86 3 251 251 CD cord-290231-4m9lj0uq 86 4 ; ; : cord-290231-4m9lj0uq 86 5 and and CC cord-290231-4m9lj0uq 86 6 MVE MVE NNP cord-290231-4m9lj0uq 86 7 17 17 CD cord-290231-4m9lj0uq 86 8 , , , cord-290231-4m9lj0uq 86 9 amino amino JJ cord-290231-4m9lj0uq 86 10 acids acid NNS cord-290231-4m9lj0uq 86 11 356 356 CD cord-290231-4m9lj0uq 86 12 - - HYPH cord-290231-4m9lj0uq 86 13 376 376 CD cord-290231-4m9lj0uq 86 14 . . . cord-290231-4m9lj0uq 87 1 The the DT cord-290231-4m9lj0uq 87 2 sequence sequence NN cord-290231-4m9lj0uq 87 3 and and CC cord-290231-4m9lj0uq 87 4 characteristics characteristic NNS cord-290231-4m9lj0uq 87 5 of of IN cord-290231-4m9lj0uq 87 6 these these DT cord-290231-4m9lj0uq 87 7 antipeptides antipeptide NNS cord-290231-4m9lj0uq 87 8 have have VBP cord-290231-4m9lj0uq 87 9 been be VBN cord-290231-4m9lj0uq 87 10 published publish VBN cord-290231-4m9lj0uq 87 11 previously previously RB cord-290231-4m9lj0uq 87 12 ( ( -LRB- cord-290231-4m9lj0uq 87 13 Roehrig Roehrig NNP cord-290231-4m9lj0uq 87 14 eta/. eta/. NN cord-290231-4m9lj0uq 87 15 , , , cord-290231-4m9lj0uq 87 16 1989 1989 CD cord-290231-4m9lj0uq 87 17 ) ) -RRB- cord-290231-4m9lj0uq 87 18 . . . cord-290231-4m9lj0uq 88 1 After after IN cord-290231-4m9lj0uq 88 2 1 1 CD cord-290231-4m9lj0uq 88 3 hr hr NN cord-290231-4m9lj0uq 88 4 at at IN cord-290231-4m9lj0uq 88 5 37 37 CD cord-290231-4m9lj0uq 88 6 " " '' cord-290231-4m9lj0uq 88 7 , , , cord-290231-4m9lj0uq 88 8 goat goat NNP cord-290231-4m9lj0uq 88 9 anti anti JJ cord-290231-4m9lj0uq 88 10 - - JJ cord-290231-4m9lj0uq 88 11 mouse mouse JJ cord-290231-4m9lj0uq 88 12 alkaline alkaline JJ cord-290231-4m9lj0uq 88 13 phosphatase phosphatase NN cord-290231-4m9lj0uq 88 14 conjugate conjugate NN cord-290231-4m9lj0uq 88 15 ( ( -LRB- cord-290231-4m9lj0uq 88 16 Jackson Jackson NNP cord-290231-4m9lj0uq 88 17 lmmuno lmmuno NNP cord-290231-4m9lj0uq 88 18 Research Research NNP cord-290231-4m9lj0uq 88 19 ) ) -RRB- cord-290231-4m9lj0uq 88 20 was be VBD cord-290231-4m9lj0uq 88 21 added add VBN cord-290231-4m9lj0uq 88 22 and and CC cord-290231-4m9lj0uq 88 23 the the DT cord-290231-4m9lj0uq 88 24 immune immune JJ cord-290231-4m9lj0uq 88 25 fragments fragment NNS cord-290231-4m9lj0uq 88 26 were be VBD cord-290231-4m9lj0uq 88 27 visualized visualize VBN cord-290231-4m9lj0uq 88 28 by by IN cord-290231-4m9lj0uq 88 29 adding add VBG cord-290231-4m9lj0uq 88 30 BCIP BCIP NNP cord-290231-4m9lj0uq 88 31 / / SYM cord-290231-4m9lj0uq 88 32 NBT NBT NNP cord-290231-4m9lj0uq 88 33 substrate substrate NN cord-290231-4m9lj0uq 88 34 ( ( -LRB- cord-290231-4m9lj0uq 88 35 KPI KPI NNP cord-290231-4m9lj0uq 88 36 , , , cord-290231-4m9lj0uq 88 37 Inc. Inc. NNP cord-290231-4m9lj0uq 88 38 , , , cord-290231-4m9lj0uq 88 39 Gaithersburg Gaithersburg NNP cord-290231-4m9lj0uq 88 40 , , , cord-290231-4m9lj0uq 88 41 MD MD NNP cord-290231-4m9lj0uq 88 42 ) ) -RRB- cord-290231-4m9lj0uq 88 43 . . . cord-290231-4m9lj0uq 89 1 For for IN cord-290231-4m9lj0uq 89 2 the the DT cord-290231-4m9lj0uq 89 3 amino amino NN cord-290231-4m9lj0uq 89 4 - - HYPH cord-290231-4m9lj0uq 89 5 terminal terminal NN cord-290231-4m9lj0uq 89 6 sequencing sequencing NN cord-290231-4m9lj0uq 89 7 , , , cord-290231-4m9lj0uq 89 8 fragments fragment NNS cord-290231-4m9lj0uq 89 9 were be VBD cord-290231-4m9lj0uq 89 10 prepared prepare VBN cord-290231-4m9lj0uq 89 11 as as IN cord-290231-4m9lj0uq 89 12 described describe VBN cord-290231-4m9lj0uq 89 13 above above IN cord-290231-4m9lj0uq 89 14 except except IN cord-290231-4m9lj0uq 89 15 that that DT cord-290231-4m9lj0uq 89 16 electroblotting electroblotting NN cord-290231-4m9lj0uq 89 17 was be VBD cord-290231-4m9lj0uq 89 18 performed perform VBN cord-290231-4m9lj0uq 89 19 on on IN cord-290231-4m9lj0uq 89 20 polyvinylidene polyvinylidene NN cord-290231-4m9lj0uq 89 21 difluoride difluoride NN cord-290231-4m9lj0uq 89 22 ( ( -LRB- cord-290231-4m9lj0uq 89 23 PVDF PVDF NNP cord-290231-4m9lj0uq 89 24 ) ) -RRB- cord-290231-4m9lj0uq 89 25 membrane membrane NN cord-290231-4m9lj0uq 89 26 ( ( -LRB- cord-290231-4m9lj0uq 89 27 Immobilon Immobilon NNP cord-290231-4m9lj0uq 89 28 - - HYPH cord-290231-4m9lj0uq 89 29 P P NNP cord-290231-4m9lj0uq 89 30 , , , cord-290231-4m9lj0uq 89 31 Millipore Millipore NNP cord-290231-4m9lj0uq 89 32 , , , cord-290231-4m9lj0uq 89 33 Bedford Bedford NNP cord-290231-4m9lj0uq 89 34 , , , cord-290231-4m9lj0uq 89 35 MA MA NNP cord-290231-4m9lj0uq 89 36 ) ) -RRB- cord-290231-4m9lj0uq 89 37 using use VBG cord-290231-4m9lj0uq 89 38 CAPS cap NNS cord-290231-4m9lj0uq 89 39 transfer transfer VBP cord-290231-4m9lj0uq 89 40 buffer buffer NN cord-290231-4m9lj0uq 89 41 ( ( -LRB- cord-290231-4m9lj0uq 89 42 10 10 CD cord-290231-4m9lj0uq 89 43 mM mm CD cord-290231-4m9lj0uq 89 44 CAPS cap NNS cord-290231-4m9lj0uq 89 45 , , , cord-290231-4m9lj0uq 89 46 0.5 0.5 CD cord-290231-4m9lj0uq 89 47 mM mm CD cord-290231-4m9lj0uq 89 48 dithiothreitol dithiothreitol NN cord-290231-4m9lj0uq 89 49 , , , cord-290231-4m9lj0uq 89 50 DTT DTT NNP cord-290231-4m9lj0uq 89 51 , , , cord-290231-4m9lj0uq 89 52 and and CC cord-290231-4m9lj0uq 89 53 10 10 CD cord-290231-4m9lj0uq 89 54 % % NN cord-290231-4m9lj0uq 89 55 methanol methanol NN cord-290231-4m9lj0uq 89 56 adjusted adjust VBN cord-290231-4m9lj0uq 89 57 to to IN cord-290231-4m9lj0uq 89 58 pH ph NN cord-290231-4m9lj0uq 89 59 1 1 CD cord-290231-4m9lj0uq 89 60 1 1 CD cord-290231-4m9lj0uq 89 61 .O .o CD cord-290231-4m9lj0uq 89 62 with with IN cord-290231-4m9lj0uq 89 63 NaCH NaCH NNP cord-290231-4m9lj0uq 89 64 ) ) -RRB- cord-290231-4m9lj0uq 89 65 . . . cord-290231-4m9lj0uq 90 1 Fragments fragment NNS cord-290231-4m9lj0uq 90 2 were be VBD cord-290231-4m9lj0uq 90 3 stained stain VBN cord-290231-4m9lj0uq 90 4 with with IN cord-290231-4m9lj0uq 90 5 0.1 0.1 CD cord-290231-4m9lj0uq 90 6 % % NN cord-290231-4m9lj0uq 90 7 Coomassie Coomassie NNP cord-290231-4m9lj0uq 90 8 blue blue NN cord-290231-4m9lj0uq 90 9 ( ( -LRB- cord-290231-4m9lj0uq 90 10 Baker Baker NNP cord-290231-4m9lj0uq 90 11 G-250 G-250 NNP cord-290231-4m9lj0uq 90 12 ) ) -RRB- cord-290231-4m9lj0uq 90 13 in in IN cord-290231-4m9lj0uq 90 14 50 50 CD cord-290231-4m9lj0uq 90 15 % % NN cord-290231-4m9lj0uq 90 16 methanol methanol NN cord-290231-4m9lj0uq 90 17 and and CC cord-290231-4m9lj0uq 90 18 10 10 CD cord-290231-4m9lj0uq 90 19 % % NN cord-290231-4m9lj0uq 90 20 acetic acetic JJ cord-290231-4m9lj0uq 90 21 acid acid NN cord-290231-4m9lj0uq 90 22 . . . cord-290231-4m9lj0uq 91 1 The the DT cord-290231-4m9lj0uq 91 2 amino amino NN cord-290231-4m9lj0uq 91 3 - - HYPH cord-290231-4m9lj0uq 91 4 terminal terminal NN cord-290231-4m9lj0uq 91 5 sequences sequence NNS cord-290231-4m9lj0uq 91 6 were be VBD cord-290231-4m9lj0uq 91 7 determined determine VBN cord-290231-4m9lj0uq 91 8 either either CC cord-290231-4m9lj0uq 91 9 on on IN cord-290231-4m9lj0uq 91 10 an an DT cord-290231-4m9lj0uq 91 11 Applied Applied NNP cord-290231-4m9lj0uq 91 12 Biosystems Biosystems NNP cord-290231-4m9lj0uq 91 13 473A 473a CD cord-290231-4m9lj0uq 92 1 ( ( -LRB- cord-290231-4m9lj0uq 92 2 Foster Foster NNP cord-290231-4m9lj0uq 92 3 City City NNP cord-290231-4m9lj0uq 92 4 , , , cord-290231-4m9lj0uq 92 5 CA CA NNP cord-290231-4m9lj0uq 92 6 ) ) -RRB- cord-290231-4m9lj0uq 92 7 or or CC cord-290231-4m9lj0uq 92 8 on on IN cord-290231-4m9lj0uq 92 9 a a DT cord-290231-4m9lj0uq 92 10 Porton Porton NNP cord-290231-4m9lj0uq 92 11 Instrument Instrument NNP cord-290231-4m9lj0uq 92 12 2090 2090 CD cord-290231-4m9lj0uq 93 1 ( ( -LRB- cord-290231-4m9lj0uq 93 2 Tarzana Tarzana NNP cord-290231-4m9lj0uq 93 3 , , , cord-290231-4m9lj0uq 93 4 CA CA NNP cord-290231-4m9lj0uq 93 5 ) ) -RRB- cord-290231-4m9lj0uq 93 6 protein protein NN cord-290231-4m9lj0uq 93 7 sequencer sequencer NN cord-290231-4m9lj0uq 93 8 using use VBG cord-290231-4m9lj0uq 93 9 standard standard JJ cord-290231-4m9lj0uq 93 10 Edman Edman NNP cord-290231-4m9lj0uq 93 11 Chemistry Chemistry NNP cord-290231-4m9lj0uq 93 12 . . . cord-290231-4m9lj0uq 94 1 Plaque plaque NN cord-290231-4m9lj0uq 94 2 assay assay NN cord-290231-4m9lj0uq 94 3 of of IN cord-290231-4m9lj0uq 94 4 prM prm JJ cord-290231-4m9lj0uq 94 5 - - : cord-290231-4m9lj0uq 94 6 and and CC cord-290231-4m9lj0uq 94 7 M M NNP cord-290231-4m9lj0uq 94 8 - - HYPH cord-290231-4m9lj0uq 94 9 containing contain VBG cord-290231-4m9lj0uq 94 10 MVE MVE NNP cord-290231-4m9lj0uq 94 11 viruses virus NNS cord-290231-4m9lj0uq 94 12 after after IN cord-290231-4m9lj0uq 94 13 exposure exposure NN cord-290231-4m9lj0uq 94 14 to to IN cord-290231-4m9lj0uq 94 15 different different JJ cord-290231-4m9lj0uq 94 16 pH ph NN cord-290231-4m9lj0uq 94 17 buffers buffer NNS cord-290231-4m9lj0uq 95 1 Equal equal JJ cord-290231-4m9lj0uq 95 2 amounts amount NNS cord-290231-4m9lj0uq 95 3 of of IN cord-290231-4m9lj0uq 95 4 plaque plaque NN cord-290231-4m9lj0uq 95 5 forming form VBG cord-290231-4m9lj0uq 95 6 units unit NNS cord-290231-4m9lj0uq 95 7 , , , cord-290231-4m9lj0uq 95 8 PFUs pfus NN cord-290231-4m9lj0uq 95 9 ( ( -LRB- cord-290231-4m9lj0uq 95 10 6 6 CD cord-290231-4m9lj0uq 95 11 x x SYM cord-290231-4m9lj0uq 95 12 1 1 CD cord-290231-4m9lj0uq 95 13 O'/mI O'/mI NNP cord-290231-4m9lj0uq 95 14 for for IN cord-290231-4m9lj0uq 95 15 viruses virus NNS cord-290231-4m9lj0uq 95 16 grown grow VBN cord-290231-4m9lj0uq 95 17 in in IN cord-290231-4m9lj0uq 95 18 C6/36 C6/36 NNP cord-290231-4m9lj0uq 95 19 cells cell NNS cord-290231-4m9lj0uq 95 20 and and CC cord-290231-4m9lj0uq 95 21 4.5 4.5 CD cord-290231-4m9lj0uq 95 22 X x NN cord-290231-4m9lj0uq 95 23 1 1 CD cord-290231-4m9lj0uq 95 24 O5 o5 NN cord-290231-4m9lj0uq 95 25 for for IN cord-290231-4m9lj0uq 95 26 viruses virus NNS cord-290231-4m9lj0uq 95 27 grown grow VBN cord-290231-4m9lj0uq 95 28 in in IN cord-290231-4m9lj0uq 95 29 SW13 SW13 NNP cord-290231-4m9lj0uq 95 30 cells cell NNS cord-290231-4m9lj0uq 95 31 ) ) -RRB- cord-290231-4m9lj0uq 95 32 , , , cord-290231-4m9lj0uq 95 33 from from IN cord-290231-4m9lj0uq 95 34 the the DT cord-290231-4m9lj0uq 95 35 supernatants supernatant NNS cord-290231-4m9lj0uq 95 36 of of IN cord-290231-4m9lj0uq 95 37 ammonium ammonium NN cord-290231-4m9lj0uq 95 38 chloride chloride NN cord-290231-4m9lj0uq 95 39 - - HYPH cord-290231-4m9lj0uq 95 40 treated treat VBN cord-290231-4m9lj0uq 95 41 and and CC cord-290231-4m9lj0uq 95 42 untreated untreated JJ cord-290231-4m9lj0uq 95 43 MVE MVE NNP cord-290231-4m9lj0uq 95 44 virus virus NN cord-290231-4m9lj0uq 95 45 - - HYPH cord-290231-4m9lj0uq 95 46 infected infect VBN cord-290231-4m9lj0uq 95 47 cells cell NNS cord-290231-4m9lj0uq 95 48 were be VBD cord-290231-4m9lj0uq 95 49 incubated incubate VBN cord-290231-4m9lj0uq 95 50 in in IN cord-290231-4m9lj0uq 95 51 different different JJ cord-290231-4m9lj0uq 95 52 pH ph NN cord-290231-4m9lj0uq 95 53 buffers buffer NNS cord-290231-4m9lj0uq 95 54 , , , cord-290231-4m9lj0uq 95 55 ranging range VBG cord-290231-4m9lj0uq 95 56 from from IN cord-290231-4m9lj0uq 95 57 7.5 7.5 CD cord-290231-4m9lj0uq 95 58 to to IN cord-290231-4m9lj0uq 95 59 4.5 4.5 CD cord-290231-4m9lj0uq 95 60 , , , cord-290231-4m9lj0uq 95 61 using use VBG cord-290231-4m9lj0uq 95 62 1 1 CD cord-290231-4m9lj0uq 95 63 M M NNP cord-290231-4m9lj0uq 95 64 HEPES HEPES NNP cord-290231-4m9lj0uq 95 65 or or CC cord-290231-4m9lj0uq 95 66 1 1 CD cord-290231-4m9lj0uq 95 67 M M NNP cord-290231-4m9lj0uq 95 68 MES MES NNP cord-290231-4m9lj0uq 95 69 containing contain VBG cord-290231-4m9lj0uq 95 70 D D NNP cord-290231-4m9lj0uq 95 71 - - HYPH cord-290231-4m9lj0uq 95 72 MEM mem NN cord-290231-4m9lj0uq 95 73 . . . cord-290231-4m9lj0uq 96 1 After after IN cord-290231-4m9lj0uq 96 2 a a DT cord-290231-4m9lj0uq 96 3 15 15 CD cord-290231-4m9lj0uq 96 4 min min NN cord-290231-4m9lj0uq 96 5 - - HYPH cord-290231-4m9lj0uq 96 6 incubation incubation NN cord-290231-4m9lj0uq 96 7 at at IN cord-290231-4m9lj0uq 96 8 room room NN cord-290231-4m9lj0uq 96 9 temperature temperature NN cord-290231-4m9lj0uq 96 10 , , , cord-290231-4m9lj0uq 96 11 the the DT cord-290231-4m9lj0uq 96 12 inoculum inoculum NN cord-290231-4m9lj0uq 96 13 was be VBD cord-290231-4m9lj0uq 96 14 exponentially exponentially RB cord-290231-4m9lj0uq 96 15 diluted dilute VBN cord-290231-4m9lj0uq 96 16 in in IN cord-290231-4m9lj0uq 96 17 BA-1 BA-1 NNP cord-290231-4m9lj0uq 96 18 diluent diluent NN cord-290231-4m9lj0uq 96 19 ( ( -LRB- cord-290231-4m9lj0uq 96 20 Hunt hunt NN cord-290231-4m9lj0uq 96 21 eta/. eta/. NN cord-290231-4m9lj0uq 96 22 , , , cord-290231-4m9lj0uq 96 23 1991 1991 CD cord-290231-4m9lj0uq 96 24 ) ) -RRB- cord-290231-4m9lj0uq 96 25 and and CC cord-290231-4m9lj0uq 96 26 examined examine VBD cord-290231-4m9lj0uq 96 27 for for IN cord-290231-4m9lj0uq 96 28 infectivity infectivity NN cord-290231-4m9lj0uq 96 29 in in IN cord-290231-4m9lj0uq 96 30 Vero Vero NNP cord-290231-4m9lj0uq 96 31 cells cell NNS cord-290231-4m9lj0uq 96 32 using use VBG cord-290231-4m9lj0uq 96 33 the the DT cord-290231-4m9lj0uq 96 34 standard standard JJ cord-290231-4m9lj0uq 96 35 plaque plaque NN cord-290231-4m9lj0uq 96 36 assay assay NN cord-290231-4m9lj0uq 96 37 . . . cord-290231-4m9lj0uq 97 1 The the DT cord-290231-4m9lj0uq 97 2 relationship relationship NN cord-290231-4m9lj0uq 97 3 between between IN cord-290231-4m9lj0uq 97 4 prM prm JJ cord-290231-4m9lj0uq 97 5 processing processing NN cord-290231-4m9lj0uq 97 6 and and CC cord-290231-4m9lj0uq 97 7 the the DT cord-290231-4m9lj0uq 97 8 fusion fusion NN cord-290231-4m9lj0uq 97 9 activity activity NN cord-290231-4m9lj0uq 97 10 To to TO cord-290231-4m9lj0uq 97 11 understand understand VB cord-290231-4m9lj0uq 97 12 the the DT cord-290231-4m9lj0uq 97 13 role role NN cord-290231-4m9lj0uq 97 14 of of IN cord-290231-4m9lj0uq 97 15 prM prm JJ cord-290231-4m9lj0uq 97 16 protein protein NN cord-290231-4m9lj0uq 97 17 in in IN cord-290231-4m9lj0uq 97 18 virus virus NN cord-290231-4m9lj0uq 97 19 maturation maturation NN cord-290231-4m9lj0uq 97 20 and and CC cord-290231-4m9lj0uq 97 21 its -PRON- PRP$ cord-290231-4m9lj0uq 97 22 interaction interaction NN cord-290231-4m9lj0uq 97 23 with with IN cord-290231-4m9lj0uq 97 24 the the DT cord-290231-4m9lj0uq 97 25 E e NN cord-290231-4m9lj0uq 97 26 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 97 27 , , , cord-290231-4m9lj0uq 97 28 we -PRON- PRP cord-290231-4m9lj0uq 97 29 investigated investigate VBD cord-290231-4m9lj0uq 97 30 the the DT cord-290231-4m9lj0uq 97 31 effect effect NN cord-290231-4m9lj0uq 97 32 that that IN cord-290231-4m9lj0uq 97 33 ammonium ammonium NN cord-290231-4m9lj0uq 97 34 chloride chloride NN cord-290231-4m9lj0uq 97 35 had have VBD cord-290231-4m9lj0uq 97 36 on on IN cord-290231-4m9lj0uq 97 37 MVE MVE NNP cord-290231-4m9lj0uq 97 38 viruses virus NNS cord-290231-4m9lj0uq 97 39 grown grow VBN cord-290231-4m9lj0uq 97 40 in in IN cord-290231-4m9lj0uq 97 41 C6/36 C6/36 NNP cord-290231-4m9lj0uq 97 42 mosquito mosquito NN cord-290231-4m9lj0uq 97 43 cells cell NNS cord-290231-4m9lj0uq 97 44 . . . cord-290231-4m9lj0uq 98 1 Ammonium ammonium NN cord-290231-4m9lj0uq 98 2 chloride chloride NN cord-290231-4m9lj0uq 98 3 has have VBZ cord-290231-4m9lj0uq 98 4 been be VBN cord-290231-4m9lj0uq 98 5 shown show VBN cord-290231-4m9lj0uq 98 6 to to TO cord-290231-4m9lj0uq 98 7 accumulate accumulate VB cord-290231-4m9lj0uq 98 8 in in IN cord-290231-4m9lj0uq 98 9 the the DT cord-290231-4m9lj0uq 98 10 post post JJ cord-290231-4m9lj0uq 98 11 - - JJ cord-290231-4m9lj0uq 98 12 Golgi golgi JJ cord-290231-4m9lj0uq 98 13 acidic acidic JJ cord-290231-4m9lj0uq 98 14 vesicles vesicle NNS cord-290231-4m9lj0uq 98 15 and and CC cord-290231-4m9lj0uq 98 16 interrupt interrupt VB cord-290231-4m9lj0uq 98 17 the the DT cord-290231-4m9lj0uq 98 18 processing processing NN cord-290231-4m9lj0uq 98 19 of of IN cord-290231-4m9lj0uq 98 20 prM prm NN cord-290231-4m9lj0uq 98 21 to to IN cord-290231-4m9lj0uq 98 22 M M NNP cord-290231-4m9lj0uq 98 23 protein protein NN cord-290231-4m9lj0uq 98 24 ( ( -LRB- cord-290231-4m9lj0uq 98 25 Randolph Randolph NNP cord-290231-4m9lj0uq 98 26 eta/. eta/. CD cord-290231-4m9lj0uq 98 27 , , , cord-290231-4m9lj0uq 98 28 1990 1990 CD cord-290231-4m9lj0uq 98 29 ) ) -RRB- cord-290231-4m9lj0uq 98 30 . . . cord-290231-4m9lj0uq 99 1 The the DT cord-290231-4m9lj0uq 99 2 unprocessed unprocessed JJ cord-290231-4m9lj0uq 99 3 prM prm JJ cord-290231-4m9lj0uq 99 4 protein protein NN cord-290231-4m9lj0uq 99 5 was be VBD cord-290231-4m9lj0uq 99 6 incorporated incorporate VBN cord-290231-4m9lj0uq 99 7 into into IN cord-290231-4m9lj0uq 99 8 the the DT cord-290231-4m9lj0uq 99 9 released release VBN cord-290231-4m9lj0uq 99 10 viruses virus NNS cord-290231-4m9lj0uq 99 11 grown grow VBN cord-290231-4m9lj0uq 99 12 in in IN cord-290231-4m9lj0uq 99 13 the the DT cord-290231-4m9lj0uq 99 14 presence presence NN cord-290231-4m9lj0uq 99 15 of of IN cord-290231-4m9lj0uq 99 16 ammonium ammonium NN cord-290231-4m9lj0uq 99 17 chloride chloride NN cord-290231-4m9lj0uq 99 18 at at IN cord-290231-4m9lj0uq 99 19 6 6 CD cord-290231-4m9lj0uq 99 20 days day NNS cord-290231-4m9lj0uq 99 21 p.i p.i NNP cord-290231-4m9lj0uq 99 22 . . . cord-290231-4m9lj0uq 100 1 ( ( -LRB- cord-290231-4m9lj0uq 100 2 Fig Fig NNP cord-290231-4m9lj0uq 100 3 . . . cord-290231-4m9lj0uq 101 1 1 1 LS cord-290231-4m9lj0uq 101 2 A A NNP cord-290231-4m9lj0uq 101 3 , , , cord-290231-4m9lj0uq 101 4 lane lane NN cord-290231-4m9lj0uq 101 5 2 2 CD cord-290231-4m9lj0uq 101 6 ) ) -RRB- cord-290231-4m9lj0uq 101 7 . . . cord-290231-4m9lj0uq 102 1 When when WRB cord-290231-4m9lj0uq 102 2 ammonium ammonium NN cord-290231-4m9lj0uq 102 3 chloride chloride NN cord-290231-4m9lj0uq 102 4 was be VBD cord-290231-4m9lj0uq 102 5 removed remove VBN cord-290231-4m9lj0uq 102 6 , , , cord-290231-4m9lj0uq 102 7 the the DT cord-290231-4m9lj0uq 102 8 cells cell NNS cord-290231-4m9lj0uq 102 9 partially partially RB cord-290231-4m9lj0uq 102 10 recovered recover VBN cord-290231-4m9lj0uq 102 11 , , , cord-290231-4m9lj0uq 102 12 and and CC cord-290231-4m9lj0uq 102 13 the the DT cord-290231-4m9lj0uq 102 14 normal normal JJ cord-290231-4m9lj0uq 102 15 processing processing NN cord-290231-4m9lj0uq 102 16 of of IN cord-290231-4m9lj0uq 102 17 the the DT cord-290231-4m9lj0uq 102 18 prM prm NN cord-290231-4m9lj0uq 102 19 to to IN cord-290231-4m9lj0uq 102 20 M M NNP cord-290231-4m9lj0uq 102 21 resumed resume VBD cord-290231-4m9lj0uq 102 22 ( ( -LRB- cord-290231-4m9lj0uq 103 1 Fig Fig NNP cord-290231-4m9lj0uq 103 2 . . . cord-290231-4m9lj0uq 103 3 lA lA NNP cord-290231-4m9lj0uq 103 4 , , , cord-290231-4m9lj0uq 103 5 _SP cord-290231-4m9lj0uq 103 6 lane lane NNP cord-290231-4m9lj0uq 103 7 3 3 CD cord-290231-4m9lj0uq 103 8 ) ) -RRB- cord-290231-4m9lj0uq 103 9 . . . cord-290231-4m9lj0uq 104 1 Since since IN cord-290231-4m9lj0uq 104 2 some some DT cord-290231-4m9lj0uq 104 3 unprocessed unprocessed JJ cord-290231-4m9lj0uq 104 4 prM prm JJ cord-290231-4m9lj0uq 104 5 proteins protein NNS cord-290231-4m9lj0uq 104 6 were be VBD cord-290231-4m9lj0uq 104 7 also also RB cord-290231-4m9lj0uq 104 8 found find VBN cord-290231-4m9lj0uq 104 9 in in IN cord-290231-4m9lj0uq 104 10 MVE MVE NNP cord-290231-4m9lj0uq 104 11 - - HYPH cord-290231-4m9lj0uq 104 12 infected infect VBN cord-290231-4m9lj0uq 104 13 cells cell NNS cord-290231-4m9lj0uq 104 14 in in IN cord-290231-4m9lj0uq 104 15 the the DT cord-290231-4m9lj0uq 104 16 absence absence NN cord-290231-4m9lj0uq 104 17 of of IN cord-290231-4m9lj0uq 104 18 ammonium ammonium NN cord-290231-4m9lj0uq 104 19 chloride chloride NN cord-290231-4m9lj0uq 104 20 ( ( -LRB- cord-290231-4m9lj0uq 104 21 Fig Fig NNP cord-290231-4m9lj0uq 104 22 . . . cord-290231-4m9lj0uq 104 23 1 1 CD cord-290231-4m9lj0uq 104 24 A A NNP cord-290231-4m9lj0uq 104 25 , , , cord-290231-4m9lj0uq 104 26 lane lane NNP cord-290231-4m9lj0uq 104 27 l l NNP cord-290231-4m9lj0uq 104 28 ) ) -RRB- cord-290231-4m9lj0uq 104 29 , , , cord-290231-4m9lj0uq 104 30 we -PRON- PRP cord-290231-4m9lj0uq 104 31 attempted attempt VBD cord-290231-4m9lj0uq 104 32 to to TO cord-290231-4m9lj0uq 104 33 quantify quantify VB cord-290231-4m9lj0uq 104 34 the the DT cord-290231-4m9lj0uq 104 35 amount amount NN cord-290231-4m9lj0uq 104 36 of of IN cord-290231-4m9lj0uq 104 37 prM prm NN cord-290231-4m9lj0uq 104 38 in in IN cord-290231-4m9lj0uq 104 39 each each DT cord-290231-4m9lj0uq 104 40 sample sample NN cord-290231-4m9lj0uq 104 41 . . . cord-290231-4m9lj0uq 105 1 This this DT cord-290231-4m9lj0uq 105 2 was be VBD cord-290231-4m9lj0uq 105 3 achieved achieve VBN cord-290231-4m9lj0uq 105 4 by by IN cord-290231-4m9lj0uq 105 5 scanning scan VBG cord-290231-4m9lj0uq 105 6 the the DT cord-290231-4m9lj0uq 105 7 stained stain VBN cord-290231-4m9lj0uq 105 8 viral viral JJ cord-290231-4m9lj0uq 105 9 proteins protein NNS cord-290231-4m9lj0uq 105 10 following follow VBG cord-290231-4m9lj0uq 105 11 separation separation NN cord-290231-4m9lj0uq 105 12 by by IN cord-290231-4m9lj0uq 105 13 SDS SDS NNP cord-290231-4m9lj0uq 105 14 - - HYPH cord-290231-4m9lj0uq 105 15 PAGE PAGE NNP cord-290231-4m9lj0uq 105 16 . . . cord-290231-4m9lj0uq 106 1 By by IN cord-290231-4m9lj0uq 106 2 using use VBG cord-290231-4m9lj0uq 106 3 nonradioactive nonradioactive JJ cord-290231-4m9lj0uq 106 4 viruses virus NNS cord-290231-4m9lj0uq 106 5 it -PRON- PRP cord-290231-4m9lj0uq 106 6 was be VBD cord-290231-4m9lj0uq 106 7 not not RB cord-290231-4m9lj0uq 106 8 always always RB cord-290231-4m9lj0uq 106 9 possible possible JJ cord-290231-4m9lj0uq 106 10 to to TO cord-290231-4m9lj0uq 106 11 clearly clearly RB cord-290231-4m9lj0uq 106 12 demonstrate demonstrate VB cord-290231-4m9lj0uq 106 13 the the DT cord-290231-4m9lj0uq 106 14 presence presence NN cord-290231-4m9lj0uq 106 15 of of IN cord-290231-4m9lj0uq 106 16 the the DT cord-290231-4m9lj0uq 106 17 small small JJ cord-290231-4m9lj0uq 106 18 M M NNP cord-290231-4m9lj0uq 106 19 protein protein NN cord-290231-4m9lj0uq 106 20 in in IN cord-290231-4m9lj0uq 106 21 minigels minigel NNS cord-290231-4m9lj0uq 106 22 . . . cord-290231-4m9lj0uq 107 1 The the DT cord-290231-4m9lj0uq 107 2 fact fact NN cord-290231-4m9lj0uq 107 3 that that IN cord-290231-4m9lj0uq 107 4 the the DT cord-290231-4m9lj0uq 107 5 ratios ratio NNS cord-290231-4m9lj0uq 107 6 of of IN cord-290231-4m9lj0uq 107 7 the the DT cord-290231-4m9lj0uq 107 8 viral viral JJ cord-290231-4m9lj0uq 107 9 structural structural JJ cord-290231-4m9lj0uq 107 10 proteins protein NNS cord-290231-4m9lj0uq 107 11 in in IN cord-290231-4m9lj0uq 107 12 the the DT cord-290231-4m9lj0uq 107 13 absence absence NN cord-290231-4m9lj0uq 107 14 of of IN cord-290231-4m9lj0uq 107 15 ammonium ammonium NN cord-290231-4m9lj0uq 107 16 chloride chloride NN cord-290231-4m9lj0uq 107 17 remain remain VBP cord-290231-4m9lj0uq 107 18 constant constant JJ cord-290231-4m9lj0uq 107 19 enabled enable VBD cord-290231-4m9lj0uq 107 20 us -PRON- PRP cord-290231-4m9lj0uq 107 21 to to TO cord-290231-4m9lj0uq 107 22 evaluate evaluate VB cord-290231-4m9lj0uq 107 23 the the DT cord-290231-4m9lj0uq 107 24 amount amount NN cord-290231-4m9lj0uq 107 25 of of IN cord-290231-4m9lj0uq 107 26 prM prm JJ cord-290231-4m9lj0uq 107 27 processing processing NN cord-290231-4m9lj0uq 107 28 indirectly indirectly RB cord-290231-4m9lj0uq 107 29 by by IN cord-290231-4m9lj0uq 107 30 calculating calculate VBG cord-290231-4m9lj0uq 107 31 its -PRON- PRP$ cord-290231-4m9lj0uq 107 32 ratio ratio NN cord-290231-4m9lj0uq 107 33 to to IN cord-290231-4m9lj0uq 107 34 other other JJ cord-290231-4m9lj0uq 107 35 structural structural JJ cord-290231-4m9lj0uq 107 36 proteins protein NNS cord-290231-4m9lj0uq 107 37 ( ( -LRB- cord-290231-4m9lj0uq 107 38 E e NN cord-290231-4m9lj0uq 107 39 or or CC cord-290231-4m9lj0uq 107 40 C C NNP cord-290231-4m9lj0uq 107 41 ) ) -RRB- cord-290231-4m9lj0uq 107 42 which which WDT cord-290231-4m9lj0uq 107 43 are be VBP cord-290231-4m9lj0uq 107 44 more more RBR cord-290231-4m9lj0uq 107 45 abundant abundant JJ cord-290231-4m9lj0uq 107 46 in in IN cord-290231-4m9lj0uq 107 47 virions virion NNS cord-290231-4m9lj0uq 107 48 . . . cord-290231-4m9lj0uq 108 1 The the DT cord-290231-4m9lj0uq 108 2 ratio ratio NN cord-290231-4m9lj0uq 108 3 of of IN cord-290231-4m9lj0uq 108 4 the the DT cord-290231-4m9lj0uq 108 5 area area NN cord-290231-4m9lj0uq 108 6 of of IN cord-290231-4m9lj0uq 108 7 each each DT cord-290231-4m9lj0uq 108 8 peak peak NN cord-290231-4m9lj0uq 108 9 corresponding correspond VBG cord-290231-4m9lj0uq 108 10 to to IN cord-290231-4m9lj0uq 108 11 C C NNP cord-290231-4m9lj0uq 108 12 or or CC cord-290231-4m9lj0uq 108 13 prM prm JJ cord-290231-4m9lj0uq 108 14 proteins protein NNS cord-290231-4m9lj0uq 108 15 to to IN cord-290231-4m9lj0uq 108 16 E E NNS cord-290231-4m9lj0uq 108 17 as as RB cord-290231-4m9lj0uq 108 18 well well RB cord-290231-4m9lj0uq 108 19 as as IN cord-290231-4m9lj0uq 108 20 prM prm JJ cord-290231-4m9lj0uq 108 21 to to IN cord-290231-4m9lj0uq 108 22 C c NN cord-290231-4m9lj0uq 108 23 protein protein NN cord-290231-4m9lj0uq 108 24 was be VBD cord-290231-4m9lj0uq 108 25 calculated calculate VBN cord-290231-4m9lj0uq 108 26 . . . cord-290231-4m9lj0uq 109 1 The the DT cord-290231-4m9lj0uq 109 2 ratio ratio NN cord-290231-4m9lj0uq 109 3 of of IN cord-290231-4m9lj0uq 109 4 C c NN cord-290231-4m9lj0uq 109 5 to to IN cord-290231-4m9lj0uq 109 6 E e NN cord-290231-4m9lj0uq 109 7 protein protein NN cord-290231-4m9lj0uq 109 8 remained remain VBD cord-290231-4m9lj0uq 109 9 constant constant JJ cord-290231-4m9lj0uq 109 10 in in IN cord-290231-4m9lj0uq 109 11 the the DT cord-290231-4m9lj0uq 109 12 presence presence NN cord-290231-4m9lj0uq 109 13 or or CC cord-290231-4m9lj0uq 109 14 absence absence NN cord-290231-4m9lj0uq 109 15 of of IN cord-290231-4m9lj0uq 109 16 ammonium ammonium NN cord-290231-4m9lj0uq 109 17 chloride chloride NN cord-290231-4m9lj0uq 109 18 ( ( -LRB- cord-290231-4m9lj0uq 109 19 data datum NNS cord-290231-4m9lj0uq 109 20 not not RB cord-290231-4m9lj0uq 109 21 shown show VBN cord-290231-4m9lj0uq 109 22 ) ) -RRB- cord-290231-4m9lj0uq 109 23 whereas whereas IN cord-290231-4m9lj0uq 109 24 the the DT cord-290231-4m9lj0uq 109 25 ratio ratio NN cord-290231-4m9lj0uq 109 26 of of IN cord-290231-4m9lj0uq 109 27 prM prm JJ cord-290231-4m9lj0uq 109 28 to to IN cord-290231-4m9lj0uq 109 29 E e NN cord-290231-4m9lj0uq 109 30 protein protein NN cord-290231-4m9lj0uq 109 31 was be VBD cord-290231-4m9lj0uq 109 32 significantly significantly RB cord-290231-4m9lj0uq 109 33 increased increase VBN cord-290231-4m9lj0uq 109 34 in in IN cord-290231-4m9lj0uq 109 35 the the DT cord-290231-4m9lj0uq 109 36 presence presence NN cord-290231-4m9lj0uq 109 37 of of IN cord-290231-4m9lj0uq 109 38 ammonium ammonium NN cord-290231-4m9lj0uq 109 39 chloride chloride NN cord-290231-4m9lj0uq 109 40 ( ( -LRB- cord-290231-4m9lj0uq 109 41 from from IN cord-290231-4m9lj0uq 109 42 0.15 0.15 CD cord-290231-4m9lj0uq 109 43 to to IN cord-290231-4m9lj0uq 109 44 0.86 0.86 CD cord-290231-4m9lj0uq 109 45 ) ) -RRB- cord-290231-4m9lj0uq 109 46 and and CC cord-290231-4m9lj0uq 109 47 reduced reduce VBN cord-290231-4m9lj0uq 109 48 to to IN cord-290231-4m9lj0uq 109 49 0.55 0.55 CD cord-290231-4m9lj0uq 109 50 following follow VBG cord-290231-4m9lj0uq 109 51 removal removal NN cord-290231-4m9lj0uq 109 52 of of IN cord-290231-4m9lj0uq 109 53 ammonium ammonium NN cord-290231-4m9lj0uq 109 54 chloride chloride NN cord-290231-4m9lj0uq 109 55 ( ( -LRB- cord-290231-4m9lj0uq 109 56 Fig Fig NNP cord-290231-4m9lj0uq 109 57 . . . cord-290231-4m9lj0uq 109 58 1A 1a NN cord-290231-4m9lj0uq 109 59 ) ) -RRB- cord-290231-4m9lj0uq 109 60 . . . cord-290231-4m9lj0uq 110 1 Similar similar JJ cord-290231-4m9lj0uq 110 2 results result NNS cord-290231-4m9lj0uq 110 3 were be VBD cord-290231-4m9lj0uq 110 4 obtained obtain VBN cord-290231-4m9lj0uq 110 5 when when WRB cord-290231-4m9lj0uq 110 6 the the DT cord-290231-4m9lj0uq 110 7 ratio ratio NN cord-290231-4m9lj0uq 110 8 of of IN cord-290231-4m9lj0uq 110 9 prM prm NN cord-290231-4m9lj0uq 110 10 to to IN cord-290231-4m9lj0uq 110 11 C C NNP cord-290231-4m9lj0uq 110 12 was be VBD cord-290231-4m9lj0uq 110 13 calculated calculate VBN cord-290231-4m9lj0uq 110 14 ( ( -LRB- cord-290231-4m9lj0uq 110 15 data datum NNS cord-290231-4m9lj0uq 110 16 not not RB cord-290231-4m9lj0uq 110 17 shown show VBN cord-290231-4m9lj0uq 110 18 ) ) -RRB- cord-290231-4m9lj0uq 110 19 . . . cord-290231-4m9lj0uq 111 1 These these DT cord-290231-4m9lj0uq 111 2 data datum NNS cord-290231-4m9lj0uq 111 3 confirm confirm VBP cord-290231-4m9lj0uq 111 4 that that IN cord-290231-4m9lj0uq 111 5 partial partial JJ cord-290231-4m9lj0uq 111 6 recovery recovery NN cord-290231-4m9lj0uq 111 7 of of IN cord-290231-4m9lj0uq 111 8 prM prm JJ cord-290231-4m9lj0uq 111 9 processing processing NN cord-290231-4m9lj0uq 111 10 has have VBZ cord-290231-4m9lj0uq 111 11 occurred occur VBN cord-290231-4m9lj0uq 111 12 once once RB cord-290231-4m9lj0uq 111 13 the the DT cord-290231-4m9lj0uq 111 14 ammonium ammonium NN cord-290231-4m9lj0uq 111 15 chloride chloride NN cord-290231-4m9lj0uq 111 16 has have VBZ cord-290231-4m9lj0uq 111 17 been be VBN cord-290231-4m9lj0uq 111 18 removed remove VBN cord-290231-4m9lj0uq 111 19 . . . cord-290231-4m9lj0uq 112 1 To to TO cord-290231-4m9lj0uq 112 2 determine determine VB cord-290231-4m9lj0uq 112 3 the the DT cord-290231-4m9lj0uq 112 4 fusion fusion NN cord-290231-4m9lj0uq 112 5 activity activity NN cord-290231-4m9lj0uq 112 6 of of IN cord-290231-4m9lj0uq 112 7 the the DT cord-290231-4m9lj0uq 112 8 viruses virus NNS cord-290231-4m9lj0uq 112 9 obtained obtain VBN cord-290231-4m9lj0uq 112 10 from from IN cord-290231-4m9lj0uq 112 11 the the DT cord-290231-4m9lj0uq 112 12 supernatants supernatant NNS cord-290231-4m9lj0uq 112 13 of of IN cord-290231-4m9lj0uq 112 14 virus virus NN cord-290231-4m9lj0uq 112 15 - - HYPH cord-290231-4m9lj0uq 112 16 infected infect VBN cord-290231-4m9lj0uq 112 17 ammonium ammonium NN cord-290231-4m9lj0uq 112 18 chloride chloride NN cord-290231-4m9lj0uq 112 19 - - HYPH cord-290231-4m9lj0uq 112 20 treated treat VBN cord-290231-4m9lj0uq 112 21 cells cell NNS cord-290231-4m9lj0uq 112 22 , , , cord-290231-4m9lj0uq 112 23 the the DT cord-290231-4m9lj0uq 112 24 FFWO FFWO NNP cord-290231-4m9lj0uq 112 25 assay assay NN cord-290231-4m9lj0uq 112 26 was be VBD cord-290231-4m9lj0uq 112 27 performed perform VBN cord-290231-4m9lj0uq 112 28 using use VBG cord-290231-4m9lj0uq 112 29 monolayers monolayer NNS cord-290231-4m9lj0uq 112 30 of of IN cord-290231-4m9lj0uq 112 31 C6/36 C6/36 NNP cord-290231-4m9lj0uq 112 32 cells cell NNS cord-290231-4m9lj0uq 112 33 as as IN cord-290231-4m9lj0uq 112 34 described describe VBN cord-290231-4m9lj0uq 112 35 previously previously RB cord-290231-4m9lj0uq 112 36 ( ( -LRB- cord-290231-4m9lj0uq 112 37 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 112 38 et et NNP cord-290231-4m9lj0uq 112 39 a/. a/. NN cord-290231-4m9lj0uq 112 40 , , , cord-290231-4m9lj0uq 112 41 199 199 CD cord-290231-4m9lj0uq 112 42 1 1 CD cord-290231-4m9lj0uq 112 43 ) ) -RRB- cord-290231-4m9lj0uq 112 44 . . . cord-290231-4m9lj0uq 113 1 No no DT cord-290231-4m9lj0uq 113 2 polykaryocyte polykaryocyte NNP cord-290231-4m9lj0uq 113 3 formation formation NN cord-290231-4m9lj0uq 113 4 was be VBD cord-290231-4m9lj0uq 113 5 observed observe VBN cord-290231-4m9lj0uq 113 6 ( ( -LRB- cord-290231-4m9lj0uq 113 7 up up IN cord-290231-4m9lj0uq 113 8 to to IN cord-290231-4m9lj0uq 113 9 200 200 CD cord-290231-4m9lj0uq 113 10 pg pg NN cord-290231-4m9lj0uq 113 11 / / SYM cord-290231-4m9lj0uq 113 12 ml ml NNS cord-290231-4m9lj0uq 113 13 of of IN cord-290231-4m9lj0uq 113 14 purified purify VBN cord-290231-4m9lj0uq 113 15 virus virus NN cord-290231-4m9lj0uq 113 16 ) ) -RRB- cord-290231-4m9lj0uq 113 17 using use VBG cord-290231-4m9lj0uq 113 18 viruses virus NNS cord-290231-4m9lj0uq 113 19 harvested harvest VBN cord-290231-4m9lj0uq 113 20 in in IN cord-290231-4m9lj0uq 113 21 the the DT cord-290231-4m9lj0uq 113 22 presence presence NN cord-290231-4m9lj0uq 113 23 of of IN cord-290231-4m9lj0uq 113 24 ammonium ammonium NN cord-290231-4m9lj0uq 113 25 chloride chloride NN cord-290231-4m9lj0uq 113 26 at at IN cord-290231-4m9lj0uq 113 27 6 6 CD cord-290231-4m9lj0uq 113 28 days day NNS cord-290231-4m9lj0uq 113 29 p.i p.i NNP cord-290231-4m9lj0uq 113 30 . . NNP cord-290231-4m9lj0uq 113 31 ; ; : cord-290231-4m9lj0uq 113 32 however however RB cord-290231-4m9lj0uq 113 33 , , , cord-290231-4m9lj0uq 113 34 when when WRB cord-290231-4m9lj0uq 113 35 ammonium ammonium NN cord-290231-4m9lj0uq 113 36 chloride chloride NN cord-290231-4m9lj0uq 113 37 was be VBD cord-290231-4m9lj0uq 113 38 removed remove VBN cord-290231-4m9lj0uq 113 39 , , , cord-290231-4m9lj0uq 113 40 the the DT cord-290231-4m9lj0uq 113 41 fusion fusion NN cord-290231-4m9lj0uq 113 42 activity activity NN cord-290231-4m9lj0uq 113 43 of of IN cord-290231-4m9lj0uq 113 44 puri puri NN cord-290231-4m9lj0uq 113 45 - - HYPH cord-290231-4m9lj0uq 113 46 fied fie VBN cord-290231-4m9lj0uq 113 47 viruses virus NNS cord-290231-4m9lj0uq 113 48 obtained obtain VBN cord-290231-4m9lj0uq 113 49 on on IN cord-290231-4m9lj0uq 113 50 Day Day NNP cord-290231-4m9lj0uq 113 51 9 9 CD cord-290231-4m9lj0uq 113 52 was be VBD cord-290231-4m9lj0uq 113 53 significantly significantly RB cord-290231-4m9lj0uq 113 54 restored restore VBN cord-290231-4m9lj0uq 113 55 ( ( -LRB- cord-290231-4m9lj0uq 113 56 Fig fig NN cord-290231-4m9lj0uq 113 57 . . . cord-290231-4m9lj0uq 113 58 1 1 CD cord-290231-4m9lj0uq 113 59 B b NN cord-290231-4m9lj0uq 113 60 ) ) -RRB- cord-290231-4m9lj0uq 113 61 . . . cord-290231-4m9lj0uq 114 1 These these DT cord-290231-4m9lj0uq 114 2 results result NNS cord-290231-4m9lj0uq 114 3 are be VBP cord-290231-4m9lj0uq 114 4 in in IN cord-290231-4m9lj0uq 114 5 agreement agreement NN cord-290231-4m9lj0uq 114 6 with with IN cord-290231-4m9lj0uq 114 7 our -PRON- PRP$ cord-290231-4m9lj0uq 114 8 previous previous JJ cord-290231-4m9lj0uq 114 9 finding finding NN cord-290231-4m9lj0uq 114 10 with with IN cord-290231-4m9lj0uq 114 11 TBE TBE NNP cord-290231-4m9lj0uq 114 12 virus virus NN cord-290231-4m9lj0uq 114 13 which which WDT cord-290231-4m9lj0uq 114 14 demonstrated demonstrate VBD cord-290231-4m9lj0uq 114 15 a a DT cord-290231-4m9lj0uq 114 16 direct direct JJ cord-290231-4m9lj0uq 114 17 relationship relationship NN cord-290231-4m9lj0uq 114 18 between between IN cord-290231-4m9lj0uq 114 19 processing processing NN cord-290231-4m9lj0uq 114 20 of of IN cord-290231-4m9lj0uq 114 21 prM prm JJ cord-290231-4m9lj0uq 114 22 and and CC cord-290231-4m9lj0uq 114 23 fusion fusion NN cord-290231-4m9lj0uq 114 24 activity activity NN cord-290231-4m9lj0uq 114 25 of of IN cord-290231-4m9lj0uq 114 26 E e NN cord-290231-4m9lj0uq 114 27 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 114 28 ( ( -LRB- cord-290231-4m9lj0uq 114 29 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 114 30 eta/. eta/. CD cord-290231-4m9lj0uq 114 31 , , , cord-290231-4m9lj0uq 114 32 1991 1991 CD cord-290231-4m9lj0uq 114 33 ) ) -RRB- cord-290231-4m9lj0uq 114 34 and and CC cord-290231-4m9lj0uq 114 35 further further RB cord-290231-4m9lj0uq 114 36 manifest manifest VBP cord-290231-4m9lj0uq 114 37 that that IN cord-290231-4m9lj0uq 114 38 both both DT cord-290231-4m9lj0uq 114 39 phenomena phenomenon NNS cord-290231-4m9lj0uq 114 40 are be VBP cord-290231-4m9lj0uq 114 41 reversible reversible JJ cord-290231-4m9lj0uq 114 42 . . . cord-290231-4m9lj0uq 115 1 Epitope Epitope NNP cord-290231-4m9lj0uq 115 2 analysis analysis NN cord-290231-4m9lj0uq 115 3 of of IN cord-290231-4m9lj0uq 115 4 the the DT cord-290231-4m9lj0uq 115 5 E E NNS cord-290231-4m9lj0uq 115 6 - - NNS cord-290231-4m9lj0uq 115 7 proteins protein NNS cord-290231-4m9lj0uq 115 8 of of IN cord-290231-4m9lj0uq 115 9 prM prm JJ cord-290231-4m9lj0uq 115 10 - - : cord-290231-4m9lj0uq 115 11 and and CC cord-290231-4m9lj0uq 115 12 Mcontaining Mcontaining NNP cord-290231-4m9lj0uq 115 13 viruses virus NNS cord-290231-4m9lj0uq 116 1 A a DT cord-290231-4m9lj0uq 116 2 capture capture NN cord-290231-4m9lj0uq 116 3 ELISA ELISA NNP cord-290231-4m9lj0uq 116 4 was be VBD cord-290231-4m9lj0uq 116 5 performed perform VBN cord-290231-4m9lj0uq 116 6 to to TO cord-290231-4m9lj0uq 116 7 analyze analyze VB cord-290231-4m9lj0uq 116 8 the the DT cord-290231-4m9lj0uq 116 9 effect effect NN cord-290231-4m9lj0uq 116 10 that that IN cord-290231-4m9lj0uq 116 11 blocking block VBG cord-290231-4m9lj0uq 116 12 the the DT cord-290231-4m9lj0uq 116 13 prM prm JJ cord-290231-4m9lj0uq 116 14 cleavage cleavage NN cord-290231-4m9lj0uq 116 15 had have VBD cord-290231-4m9lj0uq 116 16 on on IN cord-290231-4m9lj0uq 116 17 the the DT cord-290231-4m9lj0uq 116 18 expression expression NN cord-290231-4m9lj0uq 116 19 of of IN cord-290231-4m9lj0uq 116 20 E e NN cord-290231-4m9lj0uq 116 21 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 116 22 epitopes epitope NNS cord-290231-4m9lj0uq 116 23 in in IN cord-290231-4m9lj0uq 116 24 native native JJ cord-290231-4m9lj0uq 116 25 virions virion NNS cord-290231-4m9lj0uq 116 26 . . . cord-290231-4m9lj0uq 117 1 The the DT cord-290231-4m9lj0uq 117 2 reactivities reactivity NNS cord-290231-4m9lj0uq 117 3 of of IN cord-290231-4m9lj0uq 117 4 MAbs MAbs NNP cord-290231-4m9lj0uq 117 5 defining define VBG cord-290231-4m9lj0uq 117 6 nine nine CD cord-290231-4m9lj0uq 117 7 distinct distinct JJ cord-290231-4m9lj0uq 117 8 epitopes epitope NNS cord-290231-4m9lj0uq 117 9 on on IN cord-290231-4m9lj0uq 117 10 the the DT cord-290231-4m9lj0uq 117 11 MVE MVE NNP cord-290231-4m9lj0uq 117 12 E e NN cord-290231-4m9lj0uq 117 13 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 117 14 were be VBD cord-290231-4m9lj0uq 117 15 compared compare VBN cord-290231-4m9lj0uq 117 16 on on IN cord-290231-4m9lj0uq 117 17 M M NNP cord-290231-4m9lj0uq 117 18 - - HYPH cord-290231-4m9lj0uq 117 19 and and CC cord-290231-4m9lj0uq 117 20 prMcontaining prmcontaining NN cord-290231-4m9lj0uq 117 21 viruses virus NNS cord-290231-4m9lj0uq 117 22 using use VBG cord-290231-4m9lj0uq 117 23 supernatants supernatant NNS cord-290231-4m9lj0uq 117 24 of of IN cord-290231-4m9lj0uq 117 25 ammonium ammonium NN cord-290231-4m9lj0uq 117 26 chloride chloride NN cord-290231-4m9lj0uq 117 27 - - HYPH cord-290231-4m9lj0uq 117 28 treated treat VBN cord-290231-4m9lj0uq 117 29 or or CC cord-290231-4m9lj0uq 117 30 untreated untreated JJ cord-290231-4m9lj0uq 117 31 virus virus NN cord-290231-4m9lj0uq 117 32 - - HYPH cord-290231-4m9lj0uq 117 33 infected infect VBN cord-290231-4m9lj0uq 117 34 C6/36 C6/36 NNP cord-290231-4m9lj0uq 117 35 cells cell NNS cord-290231-4m9lj0uq 117 36 . . . cord-290231-4m9lj0uq 118 1 The the DT cord-290231-4m9lj0uq 118 2 antigenicity antigenicity NN cord-290231-4m9lj0uq 118 3 of of IN cord-290231-4m9lj0uq 118 4 the the DT cord-290231-4m9lj0uq 118 5 prM prM NNP cord-290231-4m9lj0uq 118 6 - - : cord-290231-4m9lj0uq 118 7 and and CC cord-290231-4m9lj0uq 118 8 M M NNP cord-290231-4m9lj0uq 118 9 - - HYPH cord-290231-4m9lj0uq 118 10 containing contain VBG cord-290231-4m9lj0uq 118 11 virus virus NN cord-290231-4m9lj0uq 118 12 supernatants supernatant NNS cord-290231-4m9lj0uq 118 13 was be VBD cord-290231-4m9lj0uq 118 14 equivalent equivalent JJ cord-290231-4m9lj0uq 118 15 in in IN cord-290231-4m9lj0uq 118 16 end end NN cord-290231-4m9lj0uq 118 17 - - HYPH cord-290231-4m9lj0uq 118 18 point point NN cord-290231-4m9lj0uq 118 19 ELISA ELISA NNP cord-290231-4m9lj0uq 118 20 antigen antigen NN cord-290231-4m9lj0uq 118 21 titration titration NN cord-290231-4m9lj0uq 118 22 using use VBG cord-290231-4m9lj0uq 118 23 a a DT cord-290231-4m9lj0uq 118 24 1:500 1:500 CD cord-290231-4m9lj0uq 118 25 dilution dilution NN cord-290231-4m9lj0uq 118 26 of of IN cord-290231-4m9lj0uq 118 27 polyclonal polyclonal JJ cord-290231-4m9lj0uq 118 28 rabbit rabbit NN cord-290231-4m9lj0uq 118 29 anti anti NNP cord-290231-4m9lj0uq 118 30 - - NNP cord-290231-4m9lj0uq 118 31 MVE MVE NNP cord-290231-4m9lj0uq 118 32 virus virus NN cord-290231-4m9lj0uq 118 33 capture capture NN cord-290231-4m9lj0uq 118 34 sera sera NNP cord-290231-4m9lj0uq 118 35 and and CC cord-290231-4m9lj0uq 118 36 anti anti NNP cord-290231-4m9lj0uq 118 37 - - NNP cord-290231-4m9lj0uq 118 38 MVE MVE NNP cord-290231-4m9lj0uq 118 39 virus virus NN cord-290231-4m9lj0uq 118 40 mouse mouse NN cord-290231-4m9lj0uq 118 41 hyperimmune hyperimmune NNP cord-290231-4m9lj0uq 118 42 ascitic ascitic NNP cord-290231-4m9lj0uq 118 43 fluid fluid NNP cord-290231-4m9lj0uq 118 44 ( ( -LRB- cord-290231-4m9lj0uq 118 45 HIAF HIAF NNP cord-290231-4m9lj0uq 118 46 ) ) -RRB- cord-290231-4m9lj0uq 118 47 as as IN cord-290231-4m9lj0uq 118 48 detector detector NN cord-290231-4m9lj0uq 118 49 . . . cord-290231-4m9lj0uq 119 1 Therefore therefore RB cord-290231-4m9lj0uq 119 2 , , , cord-290231-4m9lj0uq 119 3 a a DT cord-290231-4m9lj0uq 119 4 1:5 1:5 CD cord-290231-4m9lj0uq 119 5 dilution dilution NN cord-290231-4m9lj0uq 119 6 of of IN cord-290231-4m9lj0uq 119 7 each each DT cord-290231-4m9lj0uq 119 8 supernatant supernatant NN cord-290231-4m9lj0uq 119 9 was be VBD cord-290231-4m9lj0uq 119 10 used use VBN cord-290231-4m9lj0uq 119 11 in in IN cord-290231-4m9lj0uq 119 12 subsequent subsequent JJ cord-290231-4m9lj0uq 119 13 epitope epitope NN cord-290231-4m9lj0uq 119 14 mapping mapping NN cord-290231-4m9lj0uq 119 15 with with IN cord-290231-4m9lj0uq 119 16 MAbs MAbs NNP cord-290231-4m9lj0uq 119 17 . . . cord-290231-4m9lj0uq 120 1 The the DT cord-290231-4m9lj0uq 120 2 equivalent equivalent JJ cord-290231-4m9lj0uq 120 3 antigenicity antigenicity NN cord-290231-4m9lj0uq 120 4 was be VBD cord-290231-4m9lj0uq 120 5 confirmed confirm VBN cord-290231-4m9lj0uq 120 6 by by IN cord-290231-4m9lj0uq 120 7 end end NN cord-290231-4m9lj0uq 120 8 - - HYPH cord-290231-4m9lj0uq 120 9 point point NN cord-290231-4m9lj0uq 120 10 ELISA ELISA NNP cord-290231-4m9lj0uq 120 11 titration titration NN cord-290231-4m9lj0uq 120 12 of of IN cord-290231-4m9lj0uq 120 13 the the DT cord-290231-4m9lj0uq 120 14 MVE MVE NNP cord-290231-4m9lj0uq 120 15 HIAF HIAF NNP cord-290231-4m9lj0uq 120 16 in in IN cord-290231-4m9lj0uq 120 17 the the DT cord-290231-4m9lj0uq 120 18 standardized standardized JJ cord-290231-4m9lj0uq 120 19 capture capture NN cord-290231-4m9lj0uq 120 20 assay assay NN cord-290231-4m9lj0uq 120 21 ( ( -LRB- cord-290231-4m9lj0uq 120 22 Fig Fig NNP cord-290231-4m9lj0uq 120 23 . . . cord-290231-4m9lj0uq 120 24 2B 2b CD cord-290231-4m9lj0uq 120 25 ) ) -RRB- cord-290231-4m9lj0uq 120 26 . . . cord-290231-4m9lj0uq 121 1 Even even RB cord-290231-4m9lj0uq 121 2 though though IN cord-290231-4m9lj0uq 121 3 these these DT cord-290231-4m9lj0uq 121 4 virus virus NN cord-290231-4m9lj0uq 121 5 supernatants supernatant NNS cord-290231-4m9lj0uq 121 6 had have VBD cord-290231-4m9lj0uq 121 7 equal equal JJ cord-290231-4m9lj0uq 121 8 antigenicity antigenicity NN cord-290231-4m9lj0uq 121 9 , , , cord-290231-4m9lj0uq 121 10 the the DT cord-290231-4m9lj0uq 121 11 prM prm NN cord-290231-4m9lj0uq 121 12 - - HYPH cord-290231-4m9lj0uq 121 13 containing contain VBG cord-290231-4m9lj0uq 121 14 virus virus NN cord-290231-4m9lj0uq 121 15 supernatant supernatant NN cord-290231-4m9lj0uq 121 16 was be VBD cord-290231-4m9lj0uq 121 17 1 1 CD cord-290231-4m9lj0uq 121 18 O o NN cord-290231-4m9lj0uq 121 19 - - HYPH cord-290231-4m9lj0uq 121 20 fold fold VB cord-290231-4m9lj0uq 121 21 less less RBR cord-290231-4m9lj0uq 121 22 infectious infectious JJ cord-290231-4m9lj0uq 121 23 than than IN cord-290231-4m9lj0uq 121 24 the the DT cord-290231-4m9lj0uq 121 25 M M NNP cord-290231-4m9lj0uq 121 26 - - HYPH cord-290231-4m9lj0uq 121 27 containing contain VBG cord-290231-4m9lj0uq 121 28 virus virus NN cord-290231-4m9lj0uq 121 29 supernatant supernatant VBN cord-290231-4m9lj0uq 121 30 ( ( -LRB- cord-290231-4m9lj0uq 121 31 3.9 3.9 CD cord-290231-4m9lj0uq 121 32 X x NN cord-290231-4m9lj0uq 121 33 1 1 CD cord-290231-4m9lj0uq 121 34 O o NN cord-290231-4m9lj0uq 121 35 * * NFP cord-290231-4m9lj0uq 121 36 vs vs IN cord-290231-4m9lj0uq 121 37 2.3 2.3 CD cord-290231-4m9lj0uq 121 38 X x SYM cord-290231-4m9lj0uq 121 39 10 10 CD cord-290231-4m9lj0uq 121 40 ' ' '' cord-290231-4m9lj0uq 121 41 PFU pfu NN cord-290231-4m9lj0uq 121 42 / / SYM cord-290231-4m9lj0uq 121 43 ml ml NN cord-290231-4m9lj0uq 121 44 ) ) -RRB- cord-290231-4m9lj0uq 121 45 . . . cord-290231-4m9lj0uq 122 1 The the DT cord-290231-4m9lj0uq 122 2 reactivities reactivity NNS cord-290231-4m9lj0uq 122 3 of of IN cord-290231-4m9lj0uq 122 4 three three CD cord-290231-4m9lj0uq 122 5 E e NN cord-290231-4m9lj0uq 122 6 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 122 7 epitopes epitope NNS cord-290231-4m9lj0uq 122 8 were be VBD cord-290231-4m9lj0uq 122 9 significantly significantly RB cord-290231-4m9lj0uq 122 10 reduced reduce VBN cord-290231-4m9lj0uq 122 11 in in IN cord-290231-4m9lj0uq 122 12 prM prm NN cord-290231-4m9lj0uq 122 13 - - HYPH cord-290231-4m9lj0uq 122 14 containing contain VBG cord-290231-4m9lj0uq 122 15 viruses virus NNS cord-290231-4m9lj0uq 122 16 ( ( -LRB- cord-290231-4m9lj0uq 122 17 Fig Fig NNP cord-290231-4m9lj0uq 122 18 . . . cord-290231-4m9lj0uq 122 19 _SP cord-290231-4m9lj0uq 122 20 2C 2C NNP cord-290231-4m9lj0uq 122 21 ) ) -RRB- cord-290231-4m9lj0uq 122 22 . . . cord-290231-4m9lj0uq 123 1 Two two CD cord-290231-4m9lj0uq 123 2 of of IN cord-290231-4m9lj0uq 123 3 these these DT cord-290231-4m9lj0uq 123 4 epitopes epitope NNS cord-290231-4m9lj0uq 123 5 were be VBD cord-290231-4m9lj0uq 123 6 subcomplex subcomplex NN cord-290231-4m9lj0uq 123 7 - - HYPH cord-290231-4m9lj0uq 123 8 and and CC cord-290231-4m9lj0uq 123 9 complex complex JJ cord-290231-4m9lj0uq 123 10 - - HYPH cord-290231-4m9lj0uq 123 11 reactive reactive JJ cord-290231-4m9lj0uq 123 12 ( ( -LRB- cord-290231-4m9lj0uq 123 13 E-6 E-6 NNP cord-290231-4m9lj0uq 123 14 and and CC cord-290231-4m9lj0uq 123 15 E-8 E-8 NNP cord-290231-4m9lj0uq 123 16 ) ) -RRB- cord-290231-4m9lj0uq 123 17 and and CC cord-290231-4m9lj0uq 123 18 one one CD cord-290231-4m9lj0uq 123 19 was be VBD cord-290231-4m9lj0uq 123 20 groupreactive groupreactive JJ cord-290231-4m9lj0uq 123 21 ( ( -LRB- cord-290231-4m9lj0uq 123 22 E-4b E-4b NNP cord-290231-4m9lj0uq 123 23 ) ) -RRB- cord-290231-4m9lj0uq 123 24 , , , cord-290231-4m9lj0uq 123 25 being be VBG cord-290231-4m9lj0uq 123 26 conserved conserve VBN cord-290231-4m9lj0uq 123 27 in in IN cord-290231-4m9lj0uq 123 28 all all DT cord-290231-4m9lj0uq 123 29 flaviviruses flavivirus NNS cord-290231-4m9lj0uq 124 1 ( ( -LRB- cord-290231-4m9lj0uq 124 2 Hawkes Hawkes NNP cord-290231-4m9lj0uq 124 3 et et NNP cord-290231-4m9lj0uq 124 4 al al NNP cord-290231-4m9lj0uq 124 5 . . NNP cord-290231-4m9lj0uq 124 6 , , , cord-290231-4m9lj0uq 124 7 1988 1988 CD cord-290231-4m9lj0uq 124 8 ) ) -RRB- cord-290231-4m9lj0uq 124 9 . . . cord-290231-4m9lj0uq 125 1 The the DT cord-290231-4m9lj0uq 125 2 reactivities reactivity NNS cord-290231-4m9lj0uq 125 3 of of IN cord-290231-4m9lj0uq 125 4 other other JJ cord-290231-4m9lj0uq 125 5 MVE MVE NNP cord-290231-4m9lj0uq 125 6 virus virus NN cord-290231-4m9lj0uq 125 7 - - HYPH cord-290231-4m9lj0uq 125 8 specific specific JJ cord-290231-4m9lj0uq 125 9 MAbs MAbs NNP cord-290231-4m9lj0uq 125 10 were be VBD cord-290231-4m9lj0uq 125 11 identical identical JJ cord-290231-4m9lj0uq 125 12 with with IN cord-290231-4m9lj0uq 125 13 both both DT cord-290231-4m9lj0uq 125 14 viruses virus NNS cord-290231-4m9lj0uq 125 15 ( ( -LRB- cord-290231-4m9lj0uq 125 16 Fig Fig NNP cord-290231-4m9lj0uq 125 17 . . . cord-290231-4m9lj0uq 125 18 2A 2a CD cord-290231-4m9lj0uq 125 19 ) ) -RRB- cord-290231-4m9lj0uq 125 20 . . . cord-290231-4m9lj0uq 126 1 Structural structural JJ cord-290231-4m9lj0uq 126 2 properties property NNS cord-290231-4m9lj0uq 126 3 of of IN cord-290231-4m9lj0uq 126 4 the the DT cord-290231-4m9lj0uq 126 5 epitopes epitope NNS cord-290231-4m9lj0uq 126 6 corresponding correspond VBG cord-290231-4m9lj0uq 126 7 to to IN cord-290231-4m9lj0uq 126 8 these these DT cord-290231-4m9lj0uq 126 9 MAbs MAbs NNP cord-290231-4m9lj0uq 126 10 were be VBD cord-290231-4m9lj0uq 126 11 assessed assess VBN cord-290231-4m9lj0uq 126 12 by by IN cord-290231-4m9lj0uq 126 13 comparing compare VBG cord-290231-4m9lj0uq 126 14 the the DT cord-290231-4m9lj0uq 126 15 reactivities reactivity NNS cord-290231-4m9lj0uq 126 16 of of IN cord-290231-4m9lj0uq 126 17 MAbs MAbs NNP cord-290231-4m9lj0uq 126 18 after after IN cord-290231-4m9lj0uq 126 19 incubation incubation NN cord-290231-4m9lj0uq 126 20 of of IN cord-290231-4m9lj0uq 126 21 the the DT cord-290231-4m9lj0uq 126 22 purified purify VBN cord-290231-4m9lj0uq 126 23 viruses virus NNS cord-290231-4m9lj0uq 126 24 at at IN cord-290231-4m9lj0uq 126 25 low low JJ cord-290231-4m9lj0uq 126 26 pH ph NN cord-290231-4m9lj0uq 126 27 to to IN cord-290231-4m9lj0uq 126 28 those those DT cord-290231-4m9lj0uq 126 29 of of IN cord-290231-4m9lj0uq 126 30 untreated untreated JJ cord-290231-4m9lj0uq 126 31 viruses virus NNS cord-290231-4m9lj0uq 126 32 ( ( -LRB- cord-290231-4m9lj0uq 126 33 Fig Fig NNP cord-290231-4m9lj0uq 126 34 . . . cord-290231-4m9lj0uq 126 35 3 3 CD cord-290231-4m9lj0uq 126 36 ) ) -RRB- cord-290231-4m9lj0uq 126 37 . . . cord-290231-4m9lj0uq 127 1 As as IN cord-290231-4m9lj0uq 127 2 can can MD cord-290231-4m9lj0uq 127 3 be be VB cord-290231-4m9lj0uq 127 4 seen see VBN cord-290231-4m9lj0uq 127 5 , , , cord-290231-4m9lj0uq 127 6 the the DT cord-290231-4m9lj0uq 127 7 antigenic antigenic JJ cord-290231-4m9lj0uq 127 8 reactivities reactivity NNS cord-290231-4m9lj0uq 127 9 of of IN cord-290231-4m9lj0uq 127 10 all all DT cord-290231-4m9lj0uq 127 11 MAbs MAbs NNP cord-290231-4m9lj0uq 127 12 and and CC cord-290231-4m9lj0uq 127 13 the the DT cord-290231-4m9lj0uq 127 14 HIAF HIAF NNPS cord-290231-4m9lj0uq 127 15 were be VBD cord-290231-4m9lj0uq 127 16 significantly significantly RB cord-290231-4m9lj0uq 127 17 reduced reduce VBN cord-290231-4m9lj0uq 127 18 upon upon IN cord-290231-4m9lj0uq 127 19 low low JJ cord-290231-4m9lj0uq 127 20 pH pH NNP cord-290231-4m9lj0uq 127 21 treatment treatment NN cord-290231-4m9lj0uq 127 22 of of IN cord-290231-4m9lj0uq 127 23 the the DT cord-290231-4m9lj0uq 127 24 virus virus NN cord-290231-4m9lj0uq 127 25 . . . cord-290231-4m9lj0uq 128 1 of of IN cord-290231-4m9lj0uq 128 2 the the DT cord-290231-4m9lj0uq 128 3 E-8 E-8 NNP cord-290231-4m9lj0uq 128 4 MAb MAb NNS cord-290231-4m9lj0uq 128 5 binding bind VBG cord-290231-4m9lj0uq 128 6 site site NN cord-290231-4m9lj0uq 128 7 on on IN cord-290231-4m9lj0uq 128 8 the the DT cord-290231-4m9lj0uq 128 9 E e NN cord-290231-4m9lj0uq 128 10 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 128 11 Figure figure NN cord-290231-4m9lj0uq 128 12 4A 4a CD cord-290231-4m9lj0uq 128 13 demonstrates demonstrate VBZ cord-290231-4m9lj0uq 128 14 the the DT cord-290231-4m9lj0uq 128 15 reactivities reactivity NNS cord-290231-4m9lj0uq 128 16 of of IN cord-290231-4m9lj0uq 128 17 the the DT cord-290231-4m9lj0uq 128 18 three three CD cord-290231-4m9lj0uq 128 19 MAbs MAbs NNP cord-290231-4m9lj0uq 128 20 defining define VBG cord-290231-4m9lj0uq 128 21 the the DT cord-290231-4m9lj0uq 128 22 epitopes epitope NNS cord-290231-4m9lj0uq 128 23 E-4b e-4b NN cord-290231-4m9lj0uq 128 24 , , , cord-290231-4m9lj0uq 128 25 E-6 E-6 NNP cord-290231-4m9lj0uq 128 26 , , , cord-290231-4m9lj0uq 128 27 and and CC cord-290231-4m9lj0uq 128 28 E-8 E-8 NNP cord-290231-4m9lj0uq 128 29 , , , cord-290231-4m9lj0uq 128 30 with with IN cord-290231-4m9lj0uq 128 31 the the DT cord-290231-4m9lj0uq 128 32 MVE MVE NNP cord-290231-4m9lj0uq 128 33 virus virus NN cord-290231-4m9lj0uq 128 34 E e NN cord-290231-4m9lj0uq 128 35 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 128 36 under under IN cord-290231-4m9lj0uq 128 37 reducing reduce VBG cord-290231-4m9lj0uq 128 38 and and CC cord-290231-4m9lj0uq 128 39 nonreducing nonreducing NN cord-290231-4m9lj0uq 128 40 conditions condition NNS cord-290231-4m9lj0uq 128 41 . . . cord-290231-4m9lj0uq 129 1 As as IN cord-290231-4m9lj0uq 129 2 can can MD cord-290231-4m9lj0uq 129 3 be be VB cord-290231-4m9lj0uq 129 4 seen see VBN cord-290231-4m9lj0uq 129 5 neither neither CC cord-290231-4m9lj0uq 129 6 the the DT cord-290231-4m9lj0uq 129 7 E-4b e-4b NN cord-290231-4m9lj0uq 129 8 nor nor CC cord-290231-4m9lj0uq 129 9 E-6 E-6 NNP cord-290231-4m9lj0uq 129 10 MAbs MAbs NNP cord-290231-4m9lj0uq 129 11 recognized recognize VBD cord-290231-4m9lj0uq 129 12 the the DT cord-290231-4m9lj0uq 129 13 E e NN cord-290231-4m9lj0uq 129 14 protein protein NN cord-290231-4m9lj0uq 129 15 in in IN cord-290231-4m9lj0uq 129 16 its -PRON- PRP$ cord-290231-4m9lj0uq 129 17 re- re- JJ cord-290231-4m9lj0uq 129 18 duced duced JJ cord-290231-4m9lj0uq 129 19 form form NN cord-290231-4m9lj0uq 129 20 ( ( -LRB- cord-290231-4m9lj0uq 129 21 treated treat VBN cord-290231-4m9lj0uq 129 22 with with IN cord-290231-4m9lj0uq 129 23 2-mercaptoethanol 2-mercaptoethanol CD cord-290231-4m9lj0uq 129 24 ) ) -RRB- cord-290231-4m9lj0uq 129 25 , , , cord-290231-4m9lj0uq 129 26 whereas whereas IN cord-290231-4m9lj0uq 129 27 E-8 E-8 NNP cord-290231-4m9lj0uq 129 28 Mab Mab NNP cord-290231-4m9lj0uq 129 29 was be VBD cord-290231-4m9lj0uq 129 30 still still RB cord-290231-4m9lj0uq 129 31 reactive reactive JJ cord-290231-4m9lj0uq 129 32 after after IN cord-290231-4m9lj0uq 129 33 protein protein NN cord-290231-4m9lj0uq 129 34 reduction reduction NN cord-290231-4m9lj0uq 129 35 . . . cord-290231-4m9lj0uq 130 1 This this DT cord-290231-4m9lj0uq 130 2 reactivity reactivity NN cord-290231-4m9lj0uq 130 3 was be VBD cord-290231-4m9lj0uq 130 4 further further RB cord-290231-4m9lj0uq 130 5 sustained sustain VBN cord-290231-4m9lj0uq 130 6 after after IN cord-290231-4m9lj0uq 130 7 the the DT cord-290231-4m9lj0uq 130 8 reduction reduction NN cord-290231-4m9lj0uq 130 9 and and CC cord-290231-4m9lj0uq 130 10 alkylation alkylation NN cord-290231-4m9lj0uq 130 11 of of IN cord-290231-4m9lj0uq 130 12 the the DT cord-290231-4m9lj0uq 130 13 cysteine cysteine NN cord-290231-4m9lj0uq 130 14 residues residue NNS cord-290231-4m9lj0uq 130 15 of of IN cord-290231-4m9lj0uq 130 16 the the DT cord-290231-4m9lj0uq 130 17 E e NN cord-290231-4m9lj0uq 130 18 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 130 19 using use VBG cord-290231-4m9lj0uq 130 20 DlT dlt NN cord-290231-4m9lj0uq 130 21 / / SYM cord-290231-4m9lj0uq 130 22 SDS SDS NNP cord-290231-4m9lj0uq 130 23 followed follow VBN cord-290231-4m9lj0uq 130 24 by by IN cord-290231-4m9lj0uq 130 25 iodoacetamide iodoacetamide JJ cord-290231-4m9lj0uq 130 26 treatment treatment NN cord-290231-4m9lj0uq 131 1 ( ( -LRB- cord-290231-4m9lj0uq 131 2 Johnson Johnson NNP cord-290231-4m9lj0uq 131 3 et et NNP cord-290231-4m9lj0uq 131 4 a/. a/. NNP cord-290231-4m9lj0uq 131 5 , , , cord-290231-4m9lj0uq 131 6 1990 1990 CD cord-290231-4m9lj0uq 131 7 ) ) -RRB- cord-290231-4m9lj0uq 132 1 ( ( -LRB- cord-290231-4m9lj0uq 132 2 data datum NNS cord-290231-4m9lj0uq 132 3 not not RB cord-290231-4m9lj0uq 132 4 shown show VBN cord-290231-4m9lj0uq 132 5 ) ) -RRB- cord-290231-4m9lj0uq 132 6 . . . cord-290231-4m9lj0uq 133 1 Since since IN cord-290231-4m9lj0uq 133 2 the the DT cord-290231-4m9lj0uq 133 3 reactivity reactivity NN cord-290231-4m9lj0uq 133 4 of of IN cord-290231-4m9lj0uq 133 5 the the DT cord-290231-4m9lj0uq 133 6 E-8 E-8 NNP cord-290231-4m9lj0uq 133 7 MAb MAb NNPS cord-290231-4m9lj0uq 133 8 was be VBD cord-290231-4m9lj0uq 133 9 reduced reduce VBN cord-290231-4m9lj0uq 133 10 with with IN cord-290231-4m9lj0uq 133 11 prM prm NN cord-290231-4m9lj0uq 133 12 - - HYPH cord-290231-4m9lj0uq 133 13 containing contain VBG cord-290231-4m9lj0uq 133 14 viruses virus NNS cord-290231-4m9lj0uq 133 15 and and CC cord-290231-4m9lj0uq 133 16 the the DT cord-290231-4m9lj0uq 133 17 fact fact NN cord-290231-4m9lj0uq 133 18 that that IN cord-290231-4m9lj0uq 133 19 it -PRON- PRP cord-290231-4m9lj0uq 133 20 could could MD cord-290231-4m9lj0uq 133 21 recognize recognize VB cord-290231-4m9lj0uq 133 22 the the DT cord-290231-4m9lj0uq 133 23 E e NN cord-290231-4m9lj0uq 133 24 protein protein NN cord-290231-4m9lj0uq 133 25 in in IN cord-290231-4m9lj0uq 133 26 immunoblots immunoblot NNS cord-290231-4m9lj0uq 133 27 in in IN cord-290231-4m9lj0uq 133 28 its -PRON- PRP$ cord-290231-4m9lj0uq 133 29 linear linear JJ cord-290231-4m9lj0uq 133 30 conformation conformation NN cord-290231-4m9lj0uq 133 31 , , , cord-290231-4m9lj0uq 133 32 we -PRON- PRP cord-290231-4m9lj0uq 133 33 attempted attempt VBD cord-290231-4m9lj0uq 133 34 to to TO cord-290231-4m9lj0uq 133 35 obtain obtain VB cord-290231-4m9lj0uq 133 36 more more JJR cord-290231-4m9lj0uq 133 37 information information NN cord-290231-4m9lj0uq 133 38 about about IN cord-290231-4m9lj0uq 133 39 the the DT cord-290231-4m9lj0uq 133 40 site site NN cord-290231-4m9lj0uq 133 41 involved involve VBN cord-290231-4m9lj0uq 133 42 in in IN cord-290231-4m9lj0uq 133 43 prM prm JJ cord-290231-4m9lj0uq 133 44 - - HYPH cord-290231-4m9lj0uq 133 45 E e NN cord-290231-4m9lj0uq 133 46 interaction interaction NN cord-290231-4m9lj0uq 133 47 by by IN cord-290231-4m9lj0uq 133 48 identifying identify VBG cord-290231-4m9lj0uq 133 49 the the DT cord-290231-4m9lj0uq 133 50 E-8 E-8 NNP cord-290231-4m9lj0uq 133 51 binding binding NN cord-290231-4m9lj0uq 133 52 site site NN cord-290231-4m9lj0uq 133 53 . . . cord-290231-4m9lj0uq 134 1 The the DT cord-290231-4m9lj0uq 134 2 proteolytic proteolytic JJ cord-290231-4m9lj0uq 134 3 digestion digestion NN cord-290231-4m9lj0uq 134 4 of of IN cord-290231-4m9lj0uq 134 5 the the DT cord-290231-4m9lj0uq 134 6 native native JJ cord-290231-4m9lj0uq 134 7 E e NN cord-290231-4m9lj0uq 134 8 protein protein NN cord-290231-4m9lj0uq 134 9 with with IN cord-290231-4m9lj0uq 134 10 trypsin trypsin NN cord-290231-4m9lj0uq 134 11 ( ( -LRB- cord-290231-4m9lj0uq 134 12 which which WDT cord-290231-4m9lj0uq 134 13 cleaves cleave VBZ cord-290231-4m9lj0uq 134 14 after after IN cord-290231-4m9lj0uq 134 15 K K NNP cord-290231-4m9lj0uq 134 16 or or CC cord-290231-4m9lj0uq 134 17 R R NNP cord-290231-4m9lj0uq 134 18 ) ) -RRB- cord-290231-4m9lj0uq 134 19 or or CC cord-290231-4m9lj0uq 134 20 V8 V8 NNP cord-290231-4m9lj0uq 134 21 endoproteinase endoproteinase NN cord-290231-4m9lj0uq 134 22 ( ( -LRB- cord-290231-4m9lj0uq 134 23 which which WDT cord-290231-4m9lj0uq 134 24 cleaves cleave VBZ cord-290231-4m9lj0uq 134 25 after after IN cord-290231-4m9lj0uq 134 26 E e NN cord-290231-4m9lj0uq 134 27 or or CC cord-290231-4m9lj0uq 134 28 D D NNP cord-290231-4m9lj0uq 134 29 ) ) -RRB- cord-290231-4m9lj0uq 134 30 did do VBD cord-290231-4m9lj0uq 134 31 not not RB cord-290231-4m9lj0uq 134 32 produce produce VB cord-290231-4m9lj0uq 134 33 fragments fragment NNS cord-290231-4m9lj0uq 134 34 smaller small JJR cord-290231-4m9lj0uq 134 35 than than IN cord-290231-4m9lj0uq 134 36 30 30 CD cord-290231-4m9lj0uq 134 37 kDa kDa NNS cord-290231-4m9lj0uq 134 38 that that WDT cord-290231-4m9lj0uq 134 39 were be VBD cord-290231-4m9lj0uq 134 40 reactive reactive JJ cord-290231-4m9lj0uq 134 41 with with IN cord-290231-4m9lj0uq 134 42 E-8 E-8 NNP cord-290231-4m9lj0uq 134 43 MAb MAb NNS cord-290231-4m9lj0uq 134 44 ( ( -LRB- cord-290231-4m9lj0uq 134 45 Fig Fig NNP cord-290231-4m9lj0uq 134 46 . . . cord-290231-4m9lj0uq 134 47 4B 4b CD cord-290231-4m9lj0uq 134 48 ) ) -RRB- cord-290231-4m9lj0uq 134 49 . . . cord-290231-4m9lj0uq 135 1 In in IN cord-290231-4m9lj0uq 135 2 order order NN cord-290231-4m9lj0uq 135 3 to to TO cord-290231-4m9lj0uq 135 4 produce produce VB cord-290231-4m9lj0uq 135 5 smaller small JJR cord-290231-4m9lj0uq 135 6 fragment fragment NN cord-290231-4m9lj0uq 135 7 reactive reactive JJ cord-290231-4m9lj0uq 135 8 with with IN cord-290231-4m9lj0uq 135 9 the the DT cord-290231-4m9lj0uq 135 10 E-8 E-8 NNP cord-290231-4m9lj0uq 135 11 MAb MAb NNPS cord-290231-4m9lj0uq 135 12 , , , cord-290231-4m9lj0uq 135 13 the the DT cord-290231-4m9lj0uq 135 14 MVE MVE NNP cord-290231-4m9lj0uq 135 15 virus virus NN cord-290231-4m9lj0uq 135 16 was be VBD cord-290231-4m9lj0uq 135 17 denatured denature VBN cord-290231-4m9lj0uq 135 18 by by IN cord-290231-4m9lj0uq 135 19 SDS SDS NNP cord-290231-4m9lj0uq 135 20 prior prior RB cord-290231-4m9lj0uq 135 21 to to IN cord-290231-4m9lj0uq 135 22 protease protease NN cord-290231-4m9lj0uq 135 23 digestion digestion NN cord-290231-4m9lj0uq 135 24 . . . cord-290231-4m9lj0uq 136 1 Using use VBG cord-290231-4m9lj0uq 136 2 chymotrypsin chymotrypsin NNP cord-290231-4m9lj0uq 136 3 ( ( -LRB- cord-290231-4m9lj0uq 136 4 which which WDT cord-290231-4m9lj0uq 136 5 cleaves cleave VBZ cord-290231-4m9lj0uq 136 6 after after IN cord-290231-4m9lj0uq 136 7 F F NNP cord-290231-4m9lj0uq 136 8 , , , cord-290231-4m9lj0uq 136 9 T T NNP cord-290231-4m9lj0uq 136 10 , , , cord-290231-4m9lj0uq 136 11 or or CC cord-290231-4m9lj0uq 136 12 Y Y NNP cord-290231-4m9lj0uq 136 13 ) ) -RRB- cord-290231-4m9lj0uq 136 14 or or CC cord-290231-4m9lj0uq 136 15 V8 V8 NNP cord-290231-4m9lj0uq 136 16 protease protease NN cord-290231-4m9lj0uq 136 17 , , , cord-290231-4m9lj0uq 136 18 20-and 20-and CD cord-290231-4m9lj0uq 136 19 22-kDa 22-kda CD cord-290231-4m9lj0uq 136 20 fragments fragment NNS cord-290231-4m9lj0uq 136 21 , , , cord-290231-4m9lj0uq 136 22 Fr Fr NNP cord-290231-4m9lj0uq 136 23 20 20 CD cord-290231-4m9lj0uq 136 24 and and CC cord-290231-4m9lj0uq 136 25 Fr Fr NNP cord-290231-4m9lj0uq 136 26 22 22 CD cord-290231-4m9lj0uq 136 27 , , , cord-290231-4m9lj0uq 136 28 were be VBD cord-290231-4m9lj0uq 136 29 identified identify VBN cord-290231-4m9lj0uq 136 30 , , , cord-290231-4m9lj0uq 136 31 respectively respectively RB cord-290231-4m9lj0uq 136 32 . . . cord-290231-4m9lj0uq 137 1 These these DT cord-290231-4m9lj0uq 137 2 two two CD cord-290231-4m9lj0uq 137 3 fragments fragment NNS cord-290231-4m9lj0uq 137 4 , , , cord-290231-4m9lj0uq 137 5 which which WDT cord-290231-4m9lj0uq 137 6 reacted react VBD cord-290231-4m9lj0uq 137 7 with with IN cord-290231-4m9lj0uq 137 8 E-8 E-8 NNP cord-290231-4m9lj0uq 137 9 MAb MAb NNS cord-290231-4m9lj0uq 137 10 , , , cord-290231-4m9lj0uq 137 11 were be VBD cord-290231-4m9lj0uq 137 12 also also RB cord-290231-4m9lj0uq 137 13 recog recog VBN cord-290231-4m9lj0uq 137 14 - - HYPH cord-290231-4m9lj0uq 137 15 nized nize VBN cord-290231-4m9lj0uq 137 16 by by IN cord-290231-4m9lj0uq 137 17 mouse mouse NN cord-290231-4m9lj0uq 137 18 antiserum antiserum NN cord-290231-4m9lj0uq 137 19 immunized immunize VBN cord-290231-4m9lj0uq 137 20 with with IN cord-290231-4m9lj0uq 137 21 synthetic synthetic JJ cord-290231-4m9lj0uq 137 22 peptide peptide NN cord-290231-4m9lj0uq 137 23 MVE MVE NNP cord-290231-4m9lj0uq 137 24 06 06 CD cord-290231-4m9lj0uq 138 1 ( ( -LRB- cord-290231-4m9lj0uq 138 2 Figs fig NNS cord-290231-4m9lj0uq 138 3 . . . cord-290231-4m9lj0uq 139 1 4C 4C NNP cord-290231-4m9lj0uq 139 2 and and CC cord-290231-4m9lj0uq 139 3 4D 4D NNP cord-290231-4m9lj0uq 139 4 ) ) -RRB- cord-290231-4m9lj0uq 139 5 . . . cord-290231-4m9lj0uq 140 1 A a DT cord-290231-4m9lj0uq 140 2 23-kDa 23-kda CD cord-290231-4m9lj0uq 140 3 fragment fragment NN cord-290231-4m9lj0uq 140 4 , , , cord-290231-4m9lj0uq 140 5 Fr Fr NNP cord-290231-4m9lj0uq 140 6 23 23 CD cord-290231-4m9lj0uq 140 7 , , , cord-290231-4m9lj0uq 140 8 and and CC cord-290231-4m9lj0uq 140 9 a a DT cord-290231-4m9lj0uq 140 10 19-kDa 19-kda CD cord-290231-4m9lj0uq 140 11 fragment fragment NN cord-290231-4m9lj0uq 140 12 , , , cord-290231-4m9lj0uq 140 13 Fr Fr NNP cord-290231-4m9lj0uq 140 14 19 19 CD cord-290231-4m9lj0uq 140 15 , , , cord-290231-4m9lj0uq 140 16 were be VBD cord-290231-4m9lj0uq 140 17 produced produce VBN cord-290231-4m9lj0uq 140 18 using use VBG cord-290231-4m9lj0uq 140 19 V8 V8 NNP cord-290231-4m9lj0uq 140 20 or or CC cord-290231-4m9lj0uq 140 21 chymotrypsin chymotrypsin NNP cord-290231-4m9lj0uq 140 22 , , , cord-290231-4m9lj0uq 140 23 respectively respectively RB cord-290231-4m9lj0uq 140 24 , , , cord-290231-4m9lj0uq 140 25 which which WDT cord-290231-4m9lj0uq 140 26 were be VBD cord-290231-4m9lj0uq 140 27 recognized recognize VBN cord-290231-4m9lj0uq 140 28 by by IN cord-290231-4m9lj0uq 140 29 MVE MVE NNP cord-290231-4m9lj0uq 140 30 04 04 CD cord-290231-4m9lj0uq 140 31 antibodies antibody NNS cord-290231-4m9lj0uq 140 32 but but CC cord-290231-4m9lj0uq 140 33 not not RB cord-290231-4m9lj0uq 140 34 by by IN cord-290231-4m9lj0uq 140 35 MVE MVE NNP cord-290231-4m9lj0uq 140 36 06 06 CD cord-290231-4m9lj0uq 140 37 antibodies antibody NNS cord-290231-4m9lj0uq 140 38 or or CC cord-290231-4m9lj0uq 140 39 E-8 E-8 NNP cord-290231-4m9lj0uq 140 40 MAb MAb NNS cord-290231-4m9lj0uq 140 41 . . . cord-290231-4m9lj0uq 141 1 Antisera Antisera NNP cord-290231-4m9lj0uq 141 2 prepared prepare VBD cord-290231-4m9lj0uq 141 3 against against IN cord-290231-4m9lj0uq 141 4 MVE MVE NNP cord-290231-4m9lj0uq 141 5 17 17 CD cord-290231-4m9lj0uq 141 6 also also RB cord-290231-4m9lj0uq 141 7 recognized recognize VBD cord-290231-4m9lj0uq 141 8 a a DT cord-290231-4m9lj0uq 141 9 V8 V8 NNP cord-290231-4m9lj0uq 141 10 fragment fragment NN cord-290231-4m9lj0uq 141 11 of of IN cord-290231-4m9lj0uq 141 12 16 16 CD cord-290231-4m9lj0uq 141 13 kDa kDa NNP cord-290231-4m9lj0uq 141 14 Fr Fr NNP cord-290231-4m9lj0uq 141 15 16 16 CD cord-290231-4m9lj0uq 141 16 ( ( -LRB- cord-290231-4m9lj0uq 141 17 Fig Fig NNP cord-290231-4m9lj0uq 141 18 . . . cord-290231-4m9lj0uq 141 19 _SP cord-290231-4m9lj0uq 141 20 4D 4d CD cord-290231-4m9lj0uq 141 21 ) ) -RRB- cord-290231-4m9lj0uq 141 22 . . . cord-290231-4m9lj0uq 142 1 Fr Fr NNP cord-290231-4m9lj0uq 142 2 23 23 CD cord-290231-4m9lj0uq 142 3 , , , cord-290231-4m9lj0uq 142 4 22 22 CD cord-290231-4m9lj0uq 142 5 , , , cord-290231-4m9lj0uq 142 6 16 16 CD cord-290231-4m9lj0uq 142 7 , , , cord-290231-4m9lj0uq 142 8 and and CC cord-290231-4m9lj0uq 142 9 14 14 CD cord-290231-4m9lj0uq 142 10 were be VBD cord-290231-4m9lj0uq 142 11 sequenced sequence VBN cord-290231-4m9lj0uq 142 12 at at IN cord-290231-4m9lj0uq 142 13 their -PRON- PRP$ cord-290231-4m9lj0uq 142 14 amino amino NN cord-290231-4m9lj0uq 142 15 - - HYPH cord-290231-4m9lj0uq 142 16 terminal terminal NN cord-290231-4m9lj0uq 142 17 ends end NNS cord-290231-4m9lj0uq 142 18 and and CC cord-290231-4m9lj0uq 142 19 the the DT cord-290231-4m9lj0uq 142 20 results result NNS cord-290231-4m9lj0uq 142 21 are be VBP cord-290231-4m9lj0uq 142 22 shown show VBN cord-290231-4m9lj0uq 142 23 in in IN cord-290231-4m9lj0uq 142 24 Ta Ta NNP cord-290231-4m9lj0uq 142 25 ( ( -LRB- cord-290231-4m9lj0uq 142 26 Dalgarno Dalgarno NNP cord-290231-4m9lj0uq 142 27 et et NNP cord-290231-4m9lj0uq 142 28 a/. a/. NN cord-290231-4m9lj0uq 142 29 , , , cord-290231-4m9lj0uq 142 30 1986 1986 CD cord-290231-4m9lj0uq 142 31 ) ) -RRB- cord-290231-4m9lj0uq 142 32 predict predict VBP cord-290231-4m9lj0uq 142 33 only only RB cord-290231-4m9lj0uq 142 34 one one CD cord-290231-4m9lj0uq 142 35 potential potential JJ cord-290231-4m9lj0uq 142 36 Nlinked Nlinked NNP cord-290231-4m9lj0uq 142 37 glycosylation glycosylation NN cord-290231-4m9lj0uq 142 38 site site NN cord-290231-4m9lj0uq 142 39 on on IN cord-290231-4m9lj0uq 142 40 the the DT cord-290231-4m9lj0uq 142 41 E e NN cord-290231-4m9lj0uq 142 42 protein protein NN cord-290231-4m9lj0uq 142 43 at at IN cord-290231-4m9lj0uq 142 44 position position NN cord-290231-4m9lj0uq 142 45 N N NNP cord-290231-4m9lj0uq 142 46 - - HYPH cord-290231-4m9lj0uq 142 47 l l NNP cord-290231-4m9lj0uq 142 48 54 54 CD cord-290231-4m9lj0uq 142 49 and and CC cord-290231-4m9lj0uq 142 50 the the DT cord-290231-4m9lj0uq 142 51 fact fact NN cord-290231-4m9lj0uq 142 52 that that IN cord-290231-4m9lj0uq 142 53 this this DT cord-290231-4m9lj0uq 142 54 site site NN cord-290231-4m9lj0uq 142 55 might may MD cord-290231-4m9lj0uq 142 56 contain contain VB cord-290231-4m9lj0uq 142 57 carbohydrate carbohydrate NN cord-290231-4m9lj0uq 142 58 of of IN cord-290231-4m9lj0uq 142 59 the the DT cord-290231-4m9lj0uq 142 60 complex complex JJ cord-290231-4m9lj0uq 142 61 type type NN cord-290231-4m9lj0uq 142 62 ( ( -LRB- cord-290231-4m9lj0uq 142 63 endo endo NN cord-290231-4m9lj0uq 142 64 H H NNP cord-290231-4m9lj0uq 142 65 resistant resistant JJ cord-290231-4m9lj0uq 142 66 ) ) -RRB- cord-290231-4m9lj0uq 143 1 ( ( -LRB- cord-290231-4m9lj0uq 143 2 Winkler Winkler NNP cord-290231-4m9lj0uq 144 1 Because because IN cord-290231-4m9lj0uq 144 2 it -PRON- PRP cord-290231-4m9lj0uq 144 3 has have VBZ cord-290231-4m9lj0uq 144 4 been be VBN cord-290231-4m9lj0uq 144 5 shown show VBN cord-290231-4m9lj0uq 144 6 with with IN cord-290231-4m9lj0uq 144 7 West West NNP cord-290231-4m9lj0uq 144 8 Nile Nile NNP cord-290231-4m9lj0uq 144 9 virus virus NN cord-290231-4m9lj0uq 144 10 that that WDT cord-290231-4m9lj0uq 144 11 the the DT cord-290231-4m9lj0uq 144 12 E e NN cord-290231-4m9lj0uq 144 13 and and CC cord-290231-4m9lj0uq 144 14 prM prm JJ cord-290231-4m9lj0uq 144 15 glycoproteins glycoprotein NNS cord-290231-4m9lj0uq 144 16 remain remain VBP cord-290231-4m9lj0uq 144 17 as as IN cord-290231-4m9lj0uq 144 18 heterodimers heterodimer NNS cord-290231-4m9lj0uq 144 19 during during IN cord-290231-4m9lj0uq 144 20 transport transport NN cord-290231-4m9lj0uq 144 21 and and CC cord-290231-4m9lj0uq 144 22 maturation maturation NN cord-290231-4m9lj0uq 144 23 through through IN cord-290231-4m9lj0uq 144 24 acidic acidic JJ cord-290231-4m9lj0uq 144 25 compartments compartment NNS cord-290231-4m9lj0uq 144 26 of of IN cord-290231-4m9lj0uq 144 27 the the DT cord-290231-4m9lj0uq 144 28 exocytic exocytic JJ cord-290231-4m9lj0uq 144 29 route route NN cord-290231-4m9lj0uq 144 30 ( ( -LRB- cord-290231-4m9lj0uq 144 31 Wengler Wengler NNP cord-290231-4m9lj0uq 144 32 and and CC cord-290231-4m9lj0uq 144 33 Wengler Wengler NNP cord-290231-4m9lj0uq 144 34 , , , cord-290231-4m9lj0uq 144 35 1989 1989 CD cord-290231-4m9lj0uq 144 36 ) ) -RRB- cord-290231-4m9lj0uq 144 37 , , , cord-290231-4m9lj0uq 144 38 we -PRON- PRP cord-290231-4m9lj0uq 144 39 compared compare VBD cord-290231-4m9lj0uq 144 40 the the DT cord-290231-4m9lj0uq 144 41 infectivity infectivity NN cord-290231-4m9lj0uq 144 42 of of IN cord-290231-4m9lj0uq 144 43 the the DT cord-290231-4m9lj0uq 144 44 prM prm NN cord-290231-4m9lj0uq 144 45 - - HYPH cord-290231-4m9lj0uq 144 46 containing contain VBG cord-290231-4m9lj0uq 144 47 MVE MVE NNP cord-290231-4m9lj0uq 144 48 virus virus NN cord-290231-4m9lj0uq 144 49 with with IN cord-290231-4m9lj0uq 144 50 that that DT cord-290231-4m9lj0uq 144 51 of of IN cord-290231-4m9lj0uq 144 52 the the DT cord-290231-4m9lj0uq 144 53 M M NNP cord-290231-4m9lj0uq 144 54 - - HYPH cord-290231-4m9lj0uq 144 55 containing contain VBG cord-290231-4m9lj0uq 144 56 MVE MVE NNP cord-290231-4m9lj0uq 144 57 virus virus NN cord-290231-4m9lj0uq 144 58 following follow VBG cord-290231-4m9lj0uq 144 59 virus virus NN cord-290231-4m9lj0uq 144 60 exposure exposure NN cord-290231-4m9lj0uq 144 61 to to IN cord-290231-4m9lj0uq 144 62 acidic acidic JJ cord-290231-4m9lj0uq 144 63 conditions condition NNS cord-290231-4m9lj0uq 144 64 . . . cord-290231-4m9lj0uq 145 1 Equal equal JJ cord-290231-4m9lj0uq 145 2 PFUs pfus NN cord-290231-4m9lj0uq 145 3 of of IN cord-290231-4m9lj0uq 145 4 the the DT cord-290231-4m9lj0uq 145 5 prM prM NNP cord-290231-4m9lj0uq 145 6 - - : cord-290231-4m9lj0uq 145 7 and and CC cord-290231-4m9lj0uq 145 8 M M NNP cord-290231-4m9lj0uq 145 9 - - HYPH cord-290231-4m9lj0uq 145 10 virus virus NN cord-290231-4m9lj0uq 145 11 containing contain VBG cord-290231-4m9lj0uq 145 12 supernatants supernatant NNS cord-290231-4m9lj0uq 145 13 from from IN cord-290231-4m9lj0uq 145 14 C6/36 C6/36 NNP cord-290231-4m9lj0uq 145 15 or or CC cord-290231-4m9lj0uq 145 16 SW1 SW1 NNP cord-290231-4m9lj0uq 145 17 3 3 CD cord-290231-4m9lj0uq 145 18 cells cell NNS cord-290231-4m9lj0uq 145 19 were be VBD cord-290231-4m9lj0uq 145 20 subjected subject VBN cord-290231-4m9lj0uq 145 21 to to IN cord-290231-4m9lj0uq 145 22 environments environment NNS cord-290231-4m9lj0uq 145 23 of of IN cord-290231-4m9lj0uq 145 24 different different JJ cord-290231-4m9lj0uq 145 25 pH ph NN cord-290231-4m9lj0uq 145 26 values value NNS cord-290231-4m9lj0uq 145 27 and and CC cord-290231-4m9lj0uq 145 28 their -PRON- PRP$ cord-290231-4m9lj0uq 145 29 resultant resultant JJ cord-290231-4m9lj0uq 145 30 infectivity infectivity NN cord-290231-4m9lj0uq 145 31 in in IN cord-290231-4m9lj0uq 145 32 a a DT cord-290231-4m9lj0uq 145 33 plaque plaque NN cord-290231-4m9lj0uq 145 34 assay assay NN cord-290231-4m9lj0uq 145 35 was be VBD cord-290231-4m9lj0uq 145 36 examined examine VBN cord-290231-4m9lj0uq 145 37 ( ( -LRB- cord-290231-4m9lj0uq 145 38 Fig Fig NNP cord-290231-4m9lj0uq 145 39 . . . cord-290231-4m9lj0uq 145 40 6 6 CD cord-290231-4m9lj0uq 145 41 ) ) -RRB- cord-290231-4m9lj0uq 145 42 . . . cord-290231-4m9lj0uq 146 1 No no DT cord-290231-4m9lj0uq 146 2 significant significant JJ cord-290231-4m9lj0uq 146 3 difference difference NN cord-290231-4m9lj0uq 146 4 in in IN cord-290231-4m9lj0uq 146 5 infectivity infectivity NN cord-290231-4m9lj0uq 146 6 of of IN cord-290231-4m9lj0uq 146 7 either either DT cord-290231-4m9lj0uq 146 8 virus virus NN cord-290231-4m9lj0uq 146 9 preparation preparation NN cord-290231-4m9lj0uq 146 10 was be VBD cord-290231-4m9lj0uq 146 11 observed observe VBN cord-290231-4m9lj0uq 146 12 after after IN cord-290231-4m9lj0uq 146 13 the the DT cord-290231-4m9lj0uq 146 14 incubation incubation NN cord-290231-4m9lj0uq 146 15 of of IN cord-290231-4m9lj0uq 146 16 viruses virus NNS cord-290231-4m9lj0uq 146 17 at at IN cord-290231-4m9lj0uq 146 18 pH pH NNP cord-290231-4m9lj0uq 146 19 values value NNS cord-290231-4m9lj0uq 146 20 between between IN cord-290231-4m9lj0uq 146 21 7.5 7.5 CD cord-290231-4m9lj0uq 146 22 and and CC cord-290231-4m9lj0uq 146 23 6.5 6.5 CD cord-290231-4m9lj0uq 146 24 . . . cord-290231-4m9lj0uq 147 1 Upon upon IN cord-290231-4m9lj0uq 147 2 incubation incubation NN cord-290231-4m9lj0uq 147 3 at at IN cord-290231-4m9lj0uq 147 4 pH pH NNP cord-290231-4m9lj0uq 147 5 6.0 6.0 CD cord-290231-4m9lj0uq 147 6 , , , cord-290231-4m9lj0uq 147 7 the the DT cord-290231-4m9lj0uq 147 8 infectivity infectivity NN cord-290231-4m9lj0uq 147 9 of of IN cord-290231-4m9lj0uq 147 10 both both CC cord-290231-4m9lj0uq 147 11 M M NNP cord-290231-4m9lj0uq 147 12 - - HYPH cord-290231-4m9lj0uq 147 13 containing contain VBG cord-290231-4m9lj0uq 147 14 viruses virus NNS cord-290231-4m9lj0uq 147 15 ( ( -LRB- cord-290231-4m9lj0uq 147 16 grown grow VBN cord-290231-4m9lj0uq 147 17 in in IN cord-290231-4m9lj0uq 147 18 C6/36 C6/36 NNP cord-290231-4m9lj0uq 147 19 or or CC cord-290231-4m9lj0uq 147 20 SW1 SW1 NNP cord-290231-4m9lj0uq 147 21 3 3 CD cord-290231-4m9lj0uq 147 22 cells cell NNS cord-290231-4m9lj0uq 147 23 ) ) -RRB- cord-290231-4m9lj0uq 147 24 was be VBD cord-290231-4m9lj0uq 147 25 drastically drastically RB cord-290231-4m9lj0uq 147 26 abolished abolish VBN cord-290231-4m9lj0uq 147 27 ; ; : cord-290231-4m9lj0uq 147 28 however however RB cord-290231-4m9lj0uq 147 29 , , , cord-290231-4m9lj0uq 147 30 this this DT cord-290231-4m9lj0uq 147 31 loss loss NN cord-290231-4m9lj0uq 147 32 of of IN cord-290231-4m9lj0uq 147 33 infectivity infectivity NN cord-290231-4m9lj0uq 147 34 was be VBD cord-290231-4m9lj0uq 147 35 less less RBR cord-290231-4m9lj0uq 147 36 dramatic dramatic JJ cord-290231-4m9lj0uq 147 37 for for IN cord-290231-4m9lj0uq 147 38 prMcontaining prmcontaining NN cord-290231-4m9lj0uq 147 39 viruses virus NNS cord-290231-4m9lj0uq 147 40 than than IN cord-290231-4m9lj0uq 147 41 for for IN cord-290231-4m9lj0uq 147 42 M M NNP cord-290231-4m9lj0uq 147 43 - - HYPH cord-290231-4m9lj0uq 147 44 containing contain VBG cord-290231-4m9lj0uq 147 45 viruses virus NNS cord-290231-4m9lj0uq 148 1 ( ( -LRB- cord-290231-4m9lj0uq 148 2 150fold 150fold JJ cord-290231-4m9lj0uq 148 3 loss loss NN cord-290231-4m9lj0uq 148 4 versus versus IN cord-290231-4m9lj0uq 148 5 1360-fold 1360-fold CD cord-290231-4m9lj0uq 148 6 loss loss NN cord-290231-4m9lj0uq 148 7 for for IN cord-290231-4m9lj0uq 148 8 viruses virus NNS cord-290231-4m9lj0uq 148 9 grown grow VBN cord-290231-4m9lj0uq 148 10 in in IN cord-290231-4m9lj0uq 148 11 C6/ C6/ NNP cord-290231-4m9lj0uq 148 12 36 36 CD cord-290231-4m9lj0uq 148 13 cells cell NNS cord-290231-4m9lj0uq 148 14 , , , cord-290231-4m9lj0uq 148 15 Fig Fig NNP cord-290231-4m9lj0uq 148 16 . . . cord-290231-4m9lj0uq 149 1 6A 6a CD cord-290231-4m9lj0uq 149 2 tion tion NNP cord-290231-4m9lj0uq 149 3 199 199 CD cord-290231-4m9lj0uq 149 4 ) ) -RRB- cord-290231-4m9lj0uq 149 5 or or CC cord-290231-4m9lj0uq 149 6 Fr Fr NNP cord-290231-4m9lj0uq 149 7 16 16 CD cord-290231-4m9lj0uq 149 8 ( ( -LRB- cord-290231-4m9lj0uq 149 9 starting start VBG cord-290231-4m9lj0uq 149 10 at at IN cord-290231-4m9lj0uq 149 11 position position NN cord-290231-4m9lj0uq 149 12 327 327 CD cord-290231-4m9lj0uq 149 13 ) ) -RRB- cord-290231-4m9lj0uq 149 14 , , , cord-290231-4m9lj0uq 149 15 we -PRON- PRP cord-290231-4m9lj0uq 149 16 predict predict VBP cord-290231-4m9lj0uq 149 17 its -PRON- PRP$ cord-290231-4m9lj0uq 149 18 binding binding NN cord-290231-4m9lj0uq 149 19 site site NN cord-290231-4m9lj0uq 149 20 to to TO cord-290231-4m9lj0uq 149 21 be be VB cord-290231-4m9lj0uq 149 22 between between IN cord-290231-4m9lj0uq 149 23 amino amino JJ cord-290231-4m9lj0uq 149 24 acids acid NNS cord-290231-4m9lj0uq 149 25 200 200 CD cord-290231-4m9lj0uq 149 26 and and CC cord-290231-4m9lj0uq 149 27 327 327 CD cord-290231-4m9lj0uq 149 28 on on IN cord-290231-4m9lj0uq 149 29 the the DT cord-290231-4m9lj0uq 149 30 E e NN cord-290231-4m9lj0uq 149 31 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 149 32 ( ( -LRB- cord-290231-4m9lj0uq 149 33 Fig Fig NNP cord-290231-4m9lj0uq 149 34 . . . cord-290231-4m9lj0uq 149 35 5 5 CD cord-290231-4m9lj0uq 149 36 ) ) -RRB- cord-290231-4m9lj0uq 149 37 . . . cord-290231-4m9lj0uq 150 1 Low low JJ cord-290231-4m9lj0uq 150 2 pH ph NN cord-290231-4m9lj0uq 150 3 sensitivity sensitivity NN cord-290231-4m9lj0uq 150 4 of of IN cord-290231-4m9lj0uq 150 5 prM prm JJ cord-290231-4m9lj0uq 150 6 - - : cord-290231-4m9lj0uq 150 7 and and CC cord-290231-4m9lj0uq 150 8 M M NNP cord-290231-4m9lj0uq 150 9 - - HYPH cord-290231-4m9lj0uq 150 10 containing contain VBG cord-290231-4m9lj0uq 150 11 MVE MVE NNP cord-290231-4m9lj0uq 150 12 viruses virus NNS cord-290231-4m9lj0uq 150 13 ( ( -LRB- cord-290231-4m9lj0uq 150 14 Table table NN cord-290231-4m9lj0uq 150 15 1 1 CD cord-290231-4m9lj0uq 150 16 ) ) -RRB- cord-290231-4m9lj0uq 150 17 have have VBP cord-290231-4m9lj0uq 150 18 been be VBN cord-290231-4m9lj0uq 150 19 marked mark VBN cord-290231-4m9lj0uq 150 20 by by IN cord-290231-4m9lj0uq 150 21 arrowheads arrowhead NNS cord-290231-4m9lj0uq 150 22 . . . cord-290231-4m9lj0uq 150 23 _SP cord-290231-4m9lj0uq 151 1 a a DT cord-290231-4m9lj0uq 151 2 Fragments fragment NNS cord-290231-4m9lj0uq 151 3 were be VBD cord-290231-4m9lj0uq 151 4 generated generate VBN cord-290231-4m9lj0uq 151 5 by by IN cord-290231-4m9lj0uq 151 6 V-8 V-8 NNP cord-290231-4m9lj0uq 151 7 digestion digestion NN cord-290231-4m9lj0uq 151 8 of of IN cord-290231-4m9lj0uq 151 9 SDS sds NN cord-290231-4m9lj0uq 151 10 - - HYPH cord-290231-4m9lj0uq 151 11 denatured denature VBN cord-290231-4m9lj0uq 151 12 purified purify VBN cord-290231-4m9lj0uq 151 13 MVE MVE NNP cord-290231-4m9lj0uq 151 14 virus virus NN cord-290231-4m9lj0uq 151 15 , , , cord-290231-4m9lj0uq 151 16 separated separate VBN cord-290231-4m9lj0uq 151 17 on on IN cord-290231-4m9lj0uq 151 18 12.5 12.5 CD cord-290231-4m9lj0uq 151 19 % % NN cord-290231-4m9lj0uq 151 20 SDS SDS NNP cord-290231-4m9lj0uq 151 21 gels gel NNS cord-290231-4m9lj0uq 151 22 , , , cord-290231-4m9lj0uq 151 23 and and CC cord-290231-4m9lj0uq 151 24 transferred transfer VBN cord-290231-4m9lj0uq 151 25 to to IN cord-290231-4m9lj0uq 151 26 PVDF pvdf NN cord-290231-4m9lj0uq 151 27 membranes membrane NNS cord-290231-4m9lj0uq 151 28 by by IN cord-290231-4m9lj0uq 151 29 Western western JJ cord-290231-4m9lj0uq 151 30 blotting blotting NN cord-290231-4m9lj0uq 151 31 . . . cord-290231-4m9lj0uq 152 1 Membranes membrane NNS cord-290231-4m9lj0uq 152 2 were be VBD cord-290231-4m9lj0uq 152 3 stained stain VBN cord-290231-4m9lj0uq 152 4 with with IN cord-290231-4m9lj0uq 152 5 Coomassie Coomassie NNP cord-290231-4m9lj0uq 152 6 blue blue NN cord-290231-4m9lj0uq 152 7 and and CC cord-290231-4m9lj0uq 152 8 subjected subject VBN cord-290231-4m9lj0uq 152 9 to to IN cord-290231-4m9lj0uq 152 10 amino amino NN cord-290231-4m9lj0uq 152 11 - - HYPH cord-290231-4m9lj0uq 152 12 terminal terminal NN cord-290231-4m9lj0uq 152 13 sequence sequence NN cord-290231-4m9lj0uq 152 14 analysis analysis NN cord-290231-4m9lj0uq 152 15 . . . cord-290231-4m9lj0uq 152 16 ' ' '' cord-290231-4m9lj0uq 153 1 Fragments fragment NNS cord-290231-4m9lj0uq 153 2 were be VBD cord-290231-4m9lj0uq 153 3 generated generate VBN cord-290231-4m9lj0uq 153 4 as as IN cord-290231-4m9lj0uq 153 5 described describe VBN cord-290231-4m9lj0uq 153 6 above above RB cord-290231-4m9lj0uq 153 7 , , , cord-290231-4m9lj0uq 153 8 transferred transfer VBN cord-290231-4m9lj0uq 153 9 to to IN cord-290231-4m9lj0uq 153 10 nitrocellulose nitrocellulose NN cord-290231-4m9lj0uq 153 11 , , , cord-290231-4m9lj0uq 153 12 and and CC cord-290231-4m9lj0uq 153 13 subjected subject VBN cord-290231-4m9lj0uq 153 14 to to IN cord-290231-4m9lj0uq 153 15 immune immune JJ cord-290231-4m9lj0uq 153 16 detection detection NN cord-290231-4m9lj0uq 153 17 using use VBG cord-290231-4m9lj0uq 153 18 MVE MVE NNP cord-290231-4m9lj0uq 153 19 antipeptrde antipeptrde JJ cord-290231-4m9lj0uq 153 20 antibodies antibody NNS cord-290231-4m9lj0uq 153 21 ( ( -LRB- cord-290231-4m9lj0uq 153 22 P2 P2 NNP cord-290231-4m9lj0uq 153 23 . . . cord-290231-4m9lj0uq 154 1 P4 p4 NN cord-290231-4m9lj0uq 154 2 , , , cord-290231-4m9lj0uq 154 3 P6 p6 NN cord-290231-4m9lj0uq 154 4 . . . cord-290231-4m9lj0uq 155 1 P17 p17 NN cord-290231-4m9lj0uq 155 2 ; ; : cord-290231-4m9lj0uq 155 3 for for IN cord-290231-4m9lj0uq 155 4 sequence sequence NN cord-290231-4m9lj0uq 155 5 and and CC cord-290231-4m9lj0uq 155 6 length length NN cord-290231-4m9lj0uq 155 7 see see VB cord-290231-4m9lj0uq 155 8 Materials material NNS cord-290231-4m9lj0uq 155 9 and and CC cord-290231-4m9lj0uq 155 10 Methods Methods NNPS cord-290231-4m9lj0uq 155 11 ) ) -RRB- cord-290231-4m9lj0uq 155 12 or or CC cord-290231-4m9lj0uq 155 13 Mab Mab NNP cord-290231-4m9lj0uq 155 14 E-8 E-8 NNP cord-290231-4m9lj0uq 155 15 as as IN cord-290231-4m9lj0uq 155 16 described describe VBN cord-290231-4m9lj0uq 155 17 under under IN cord-290231-4m9lj0uq 155 18 Materials material NNS cord-290231-4m9lj0uq 155 19 and and CC cord-290231-4m9lj0uq 155 20 Methods Methods NNPS cord-290231-4m9lj0uq 155 21 , , , cord-290231-4m9lj0uq 155 22 for for IN cord-290231-4m9lj0uq 155 23 viruses virus NNS cord-290231-4m9lj0uq 155 24 grown grow VBN cord-290231-4m9lj0uq 155 25 in in IN cord-290231-4m9lj0uq 155 26 SW1 SW1 NNP cord-290231-4m9lj0uq 155 27 3 3 CD cord-290231-4m9lj0uq 155 28 cells cell NNS cord-290231-4m9lj0uq 155 29 , , , cord-290231-4m9lj0uq 155 30 Fig Fig NNP cord-290231-4m9lj0uq 155 31 . . . cord-290231-4m9lj0uq 155 32 6B 6b CD cord-290231-4m9lj0uq 155 33 ) ) -RRB- cord-290231-4m9lj0uq 155 34 . . . cord-290231-4m9lj0uq 156 1 Maximal maximal JJ cord-290231-4m9lj0uq 156 2 loss loss NN cord-290231-4m9lj0uq 156 3 of of IN cord-290231-4m9lj0uq 156 4 infectivity infectivity NN cord-290231-4m9lj0uq 156 5 was be VBD cord-290231-4m9lj0uq 156 6 achieved achieve VBN cord-290231-4m9lj0uq 156 7 upon upon IN cord-290231-4m9lj0uq 156 8 incubation incubation NN cord-290231-4m9lj0uq 156 9 at at IN cord-290231-4m9lj0uq 156 10 pH pH NNP cord-290231-4m9lj0uq 156 11 4.5 4.5 CD cord-290231-4m9lj0uq 156 12 ; ; : cord-290231-4m9lj0uq 156 13 nevertheless nevertheless RB cord-290231-4m9lj0uq 156 14 , , , cord-290231-4m9lj0uq 156 15 the the DT cord-290231-4m9lj0uq 156 16 prM prm NN cord-290231-4m9lj0uq 156 17 - - HYPH cord-290231-4m9lj0uq 156 18 containing contain VBG cord-290231-4m9lj0uq 156 19 viruses virus NNS cord-290231-4m9lj0uq 156 20 were be VBD cord-290231-4m9lj0uq 156 21 still still RB cord-290231-4m9lj0uq 156 22 1 1 CD cord-290231-4m9lj0uq 156 23 Ofold Ofold NNP cord-290231-4m9lj0uq 156 24 ( ( -LRB- cord-290231-4m9lj0uq 156 25 those those DT cord-290231-4m9lj0uq 156 26 grown grow VBN cord-290231-4m9lj0uq 156 27 in in IN cord-290231-4m9lj0uq 156 28 C6/36 C6/36 NNP cord-290231-4m9lj0uq 156 29 cells cell NNS cord-290231-4m9lj0uq 156 30 ) ) -RRB- cord-290231-4m9lj0uq 156 31 and and CC cord-290231-4m9lj0uq 156 32 400-fold 400-fold CD cord-290231-4m9lj0uq 156 33 ( ( -LRB- cord-290231-4m9lj0uq 156 34 those those DT cord-290231-4m9lj0uq 156 35 grown grow VBN cord-290231-4m9lj0uq 156 36 in in IN cord-290231-4m9lj0uq 156 37 SW1 SW1 NNP cord-290231-4m9lj0uq 156 38 3 3 CD cord-290231-4m9lj0uq 156 39 cells cell NNS cord-290231-4m9lj0uq 156 40 ) ) -RRB- cord-290231-4m9lj0uq 156 41 more more RBR cord-290231-4m9lj0uq 156 42 resistant resistant JJ cord-290231-4m9lj0uq 156 43 to to IN cord-290231-4m9lj0uq 156 44 the the DT cord-290231-4m9lj0uq 156 45 low low JJ cord-290231-4m9lj0uq 156 46 pH pH NNP cord-290231-4m9lj0uq 156 47 environment environment NN cord-290231-4m9lj0uq 156 48 than than IN cord-290231-4m9lj0uq 156 49 the the DT cord-290231-4m9lj0uq 156 50 M M NNP cord-290231-4m9lj0uq 156 51 - - HYPH cord-290231-4m9lj0uq 156 52 containing contain VBG cord-290231-4m9lj0uq 156 53 viruses virus NNS cord-290231-4m9lj0uq 156 54 . . . cord-290231-4m9lj0uq 157 1 The the DT cord-290231-4m9lj0uq 157 2 proteolytic proteolytic JJ cord-290231-4m9lj0uq 157 3 cleavage cleavage NN cord-290231-4m9lj0uq 157 4 of of IN cord-290231-4m9lj0uq 157 5 prM prm JJ cord-290231-4m9lj0uq 157 6 occurs occur NNS cord-290231-4m9lj0uq 157 7 after after IN cord-290231-4m9lj0uq 157 8 a a DT cord-290231-4m9lj0uq 157 9 dibasic dibasic NN cord-290231-4m9lj0uq 157 10 amino amino NN cord-290231-4m9lj0uq 157 11 acid acid NN cord-290231-4m9lj0uq 157 12 motif motif NN cord-290231-4m9lj0uq 157 13 , , , cord-290231-4m9lj0uq 157 14 presumably presumably RB cord-290231-4m9lj0uq 157 15 by by IN cord-290231-4m9lj0uq 157 16 a a DT cord-290231-4m9lj0uq 157 17 trypsin trypsin NN cord-290231-4m9lj0uq 157 18 - - HYPH cord-290231-4m9lj0uq 157 19 like like JJ cord-290231-4m9lj0uq 157 20 host host NN cord-290231-4m9lj0uq 157 21 enzyme enzyme NN cord-290231-4m9lj0uq 157 22 similar similar JJ cord-290231-4m9lj0uq 157 23 to to IN cord-290231-4m9lj0uq 157 24 those those DT cord-290231-4m9lj0uq 157 25 responsible responsible JJ cord-290231-4m9lj0uq 157 26 for for IN cord-290231-4m9lj0uq 157 27 normal normal JJ cord-290231-4m9lj0uq 157 28 processing processing NN cord-290231-4m9lj0uq 157 29 of of IN cord-290231-4m9lj0uq 157 30 prohormones prohormone NNS cord-290231-4m9lj0uq 157 31 ( ( -LRB- cord-290231-4m9lj0uq 157 32 Fischer Fischer NNP cord-290231-4m9lj0uq 157 33 and and CC cord-290231-4m9lj0uq 157 34 Scheller Scheller NNP cord-290231-4m9lj0uq 157 35 , , , cord-290231-4m9lj0uq 157 36 1988 1988 CD cord-290231-4m9lj0uq 157 37 ; ; : cord-290231-4m9lj0uq 157 38 Steiner Steiner NNP cord-290231-4m9lj0uq 157 39 e2 e2 NNP cord-290231-4m9lj0uq 157 40 al al NNP cord-290231-4m9lj0uq 157 41 . . NNP cord-290231-4m9lj0uq 157 42 , , , cord-290231-4m9lj0uq 157 43 1984 1984 CD cord-290231-4m9lj0uq 157 44 ) ) -RRB- cord-290231-4m9lj0uq 157 45 . . . cord-290231-4m9lj0uq 158 1 Recently recently RB cord-290231-4m9lj0uq 158 2 , , , cord-290231-4m9lj0uq 158 3 an an DT cord-290231-4m9lj0uq 158 4 enzyme enzyme NN cord-290231-4m9lj0uq 158 5 designated designate VBN cord-290231-4m9lj0uq 158 6 paired pair VBN cord-290231-4m9lj0uq 158 7 basic basic JJ cord-290231-4m9lj0uq 158 8 amino amino NN cord-290231-4m9lj0uq 158 9 acid acid NN cord-290231-4m9lj0uq 158 10 cleaving cleaving NN cord-290231-4m9lj0uq 158 11 enzyme enzyme NN cord-290231-4m9lj0uq 158 12 ( ( -LRB- cord-290231-4m9lj0uq 158 13 PACE pace NN cord-290231-4m9lj0uq 158 14 ) ) -RRB- cord-290231-4m9lj0uq 158 15 has have VBZ cord-290231-4m9lj0uq 158 16 been be VBN cord-290231-4m9lj0uq 158 17 cloned clone VBN cord-290231-4m9lj0uq 158 18 and and CC cord-290231-4m9lj0uq 158 19 purified purify VBN cord-290231-4m9lj0uq 158 20 from from IN cord-290231-4m9lj0uq 158 21 a a DT cord-290231-4m9lj0uq 158 22 human human JJ cord-290231-4m9lj0uq 158 23 liver liver NN cord-290231-4m9lj0uq 158 24 cell cell NN cord-290231-4m9lj0uq 158 25 line line NN cord-290231-4m9lj0uq 158 26 ( ( -LRB- cord-290231-4m9lj0uq 158 27 Wise Wise NNP cord-290231-4m9lj0uq 158 28 et et NNP cord-290231-4m9lj0uq 158 29 al al NNP cord-290231-4m9lj0uq 158 30 . . NNP cord-290231-4m9lj0uq 158 31 , , , cord-290231-4m9lj0uq 158 32 1990 1990 CD cord-290231-4m9lj0uq 158 33 ) ) -RRB- cord-290231-4m9lj0uq 158 34 . . . cord-290231-4m9lj0uq 159 1 A a DT cord-290231-4m9lj0uq 159 2 similar similar JJ cord-290231-4m9lj0uq 159 3 endoprotease endoprotease NN cord-290231-4m9lj0uq 159 4 was be VBD cord-290231-4m9lj0uq 159 5 also also RB cord-290231-4m9lj0uq 159 6 isolated isolate VBN cord-290231-4m9lj0uq 159 7 from from IN cord-290231-4m9lj0uq 159 8 the the DT cord-290231-4m9lj0uq 159 9 trans trans NNP cord-290231-4m9lj0uq 159 10 - - NNP cord-290231-4m9lj0uq 159 11 Golgi Golgi NNP cord-290231-4m9lj0uq 159 12 membranes membrane NNS cord-290231-4m9lj0uq 159 13 of of IN cord-290231-4m9lj0uq 159 14 rat rat NN cord-290231-4m9lj0uq 159 15 liver liver NN cord-290231-4m9lj0uq 159 16 cells cell NNS cord-290231-4m9lj0uq 159 17 , , , cord-290231-4m9lj0uq 159 18 which which WDT cord-290231-4m9lj0uq 159 19 was be VBD cord-290231-4m9lj0uq 159 20 shown show VBN cord-290231-4m9lj0uq 159 21 to to TO cord-290231-4m9lj0uq 159 22 cleave cleave VB cord-290231-4m9lj0uq 159 23 in in RP cord-290231-4m9lj0uq 159 24 vitro vitro FW cord-290231-4m9lj0uq 159 25 the the DT cord-290231-4m9lj0uq 159 26 F F NNP cord-290231-4m9lj0uq 159 27 , , , cord-290231-4m9lj0uq 159 28 protein protein NN cord-290231-4m9lj0uq 159 29 of of IN cord-290231-4m9lj0uq 159 30 New New NNP cord-290231-4m9lj0uq 159 31 Castle Castle NNP cord-290231-4m9lj0uq 159 32 disease disease NN cord-290231-4m9lj0uq 159 33 virus virus NN cord-290231-4m9lj0uq 159 34 after after IN cord-290231-4m9lj0uq 159 35 a a DT cord-290231-4m9lj0uq 159 36 dibasic dibasic NN cord-290231-4m9lj0uq 159 37 residue residue NN cord-290231-4m9lj0uq 159 38 motif motif NN cord-290231-4m9lj0uq 159 39 ( ( -LRB- cord-290231-4m9lj0uq 159 40 Sakaguchi Sakaguchi NNP cord-290231-4m9lj0uq 159 41 et et NNP cord-290231-4m9lj0uq 159 42 al al NNP cord-290231-4m9lj0uq 159 43 . . NNP cord-290231-4m9lj0uq 159 44 , , , cord-290231-4m9lj0uq 159 45 199 199 CD cord-290231-4m9lj0uq 159 46 1 1 CD cord-290231-4m9lj0uq 159 47 ) ) -RRB- cord-290231-4m9lj0uq 159 48 . . . cord-290231-4m9lj0uq 160 1 We -PRON- PRP cord-290231-4m9lj0uq 160 2 believe believe VBP cord-290231-4m9lj0uq 160 3 that that IN cord-290231-4m9lj0uq 160 4 the the DT cord-290231-4m9lj0uq 160 5 late late JJ cord-290231-4m9lj0uq 160 6 maturational maturational JJ cord-290231-4m9lj0uq 160 7 processing processing NN cord-290231-4m9lj0uq 160 8 of of IN cord-290231-4m9lj0uq 160 9 prM prm JJ cord-290231-4m9lj0uq 160 10 at at IN cord-290231-4m9lj0uq 160 11 the the DT cord-290231-4m9lj0uq 160 12 R r NN cord-290231-4m9lj0uq 160 13 - - HYPH cord-290231-4m9lj0uq 160 14 X x NN cord-290231-4m9lj0uq 160 15 - - HYPH cord-290231-4m9lj0uq 160 16 R R NNP cord-290231-4m9lj0uq 160 17 / / SYM cord-290231-4m9lj0uq 160 18 K k NN cord-290231-4m9lj0uq 160 19 - - HYPH cord-290231-4m9lj0uq 160 20 R r NN cord-290231-4m9lj0uq 160 21 cleavage cleavage NN cord-290231-4m9lj0uq 160 22 motif motif NN cord-290231-4m9lj0uq 160 23 is be VBZ cord-290231-4m9lj0uq 160 24 the the DT cord-290231-4m9lj0uq 160 25 mechanism mechanism NN cord-290231-4m9lj0uq 160 26 used use VBN cord-290231-4m9lj0uq 160 27 to to TO cord-290231-4m9lj0uq 160 28 activate activate VB cord-290231-4m9lj0uq 160 29 the the DT cord-290231-4m9lj0uq 160 30 flavivirus flavivirus JJ cord-290231-4m9lj0uq 160 31 fusion fusion NN cord-290231-4m9lj0uq 160 32 protein protein NN cord-290231-4m9lj0uq 160 33 ( ( -LRB- cord-290231-4m9lj0uq 160 34 E e NN cord-290231-4m9lj0uq 160 35 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 160 36 ) ) -RRB- cord-290231-4m9lj0uq 160 37 . . . cord-290231-4m9lj0uq 161 1 The the DT cord-290231-4m9lj0uq 161 2 infectivity infectivity NN cord-290231-4m9lj0uq 161 3 of of IN cord-290231-4m9lj0uq 161 4 prM prm NN cord-290231-4m9lj0uq 161 5 - - HYPH cord-290231-4m9lj0uq 161 6 containing contain VBG cord-290231-4m9lj0uq 161 7 viruses virus NNS cord-290231-4m9lj0uq 161 8 ( ( -LRB- cord-290231-4m9lj0uq 161 9 grown grow VBN cord-290231-4m9lj0uq 161 10 in in IN cord-290231-4m9lj0uq 161 11 C6/36 C6/36 NNP cord-290231-4m9lj0uq 161 12 cells cell NNS cord-290231-4m9lj0uq 161 13 ) ) -RRB- cord-290231-4m9lj0uq 161 14 for for IN cord-290231-4m9lj0uq 161 15 mammalian mammalian JJ cord-290231-4m9lj0uq 161 16 cells cell NNS cord-290231-4m9lj0uq 161 17 was be VBD cord-290231-4m9lj0uq 161 18 lo lo NNP cord-290231-4m9lj0uq 161 19 - - JJ cord-290231-4m9lj0uq 161 20 fold fold VB cord-290231-4m9lj0uq 161 21 lower lower RBR cord-290231-4m9lj0uq 161 22 than than IN cord-290231-4m9lj0uq 161 23 that that DT cord-290231-4m9lj0uq 161 24 of of IN cord-290231-4m9lj0uq 161 25 the the DT cord-290231-4m9lj0uq 161 26 mature mature JJ cord-290231-4m9lj0uq 161 27 MVE MVE NNP cord-290231-4m9lj0uq 161 28 ( ( -LRB- cord-290231-4m9lj0uq 161 29 M M NNP cord-290231-4m9lj0uq 161 30 - - HYPH cord-290231-4m9lj0uq 161 31 containing contain VBG cord-290231-4m9lj0uq 161 32 ) ) -RRB- cord-290231-4m9lj0uq 161 33 viruses virus NNS cord-290231-4m9lj0uq 161 34 , , , cord-290231-4m9lj0uq 161 35 although although IN cord-290231-4m9lj0uq 161 36 the the DT cord-290231-4m9lj0uq 161 37 amount amount NN cord-290231-4m9lj0uq 161 38 of of IN cord-290231-4m9lj0uq 161 39 antigen antigen NN cord-290231-4m9lj0uq 161 40 detected detect VBN cord-290231-4m9lj0uq 161 41 by by IN cord-290231-4m9lj0uq 161 42 polyclonal polyclonal JJ cord-290231-4m9lj0uq 161 43 antibodies antibody NNS cord-290231-4m9lj0uq 161 44 was be VBD cord-290231-4m9lj0uq 161 45 similar similar JJ cord-290231-4m9lj0uq 161 46 for for IN cord-290231-4m9lj0uq 161 47 both both DT cord-290231-4m9lj0uq 161 48 viruses virus NNS cord-290231-4m9lj0uq 161 49 . . . cord-290231-4m9lj0uq 162 1 These these DT cord-290231-4m9lj0uq 162 2 results result NNS cord-290231-4m9lj0uq 162 3 indicate indicate VBP cord-290231-4m9lj0uq 162 4 that that IN cord-290231-4m9lj0uq 162 5 prM prm JJ cord-290231-4m9lj0uq 162 6 processing processing NN cord-290231-4m9lj0uq 162 7 is be VBZ cord-290231-4m9lj0uq 162 8 required require VBN cord-290231-4m9lj0uq 162 9 for for IN cord-290231-4m9lj0uq 162 10 full full JJ cord-290231-4m9lj0uq 162 11 virus virus NN cord-290231-4m9lj0uq 162 12 infectivity infectivity NN cord-290231-4m9lj0uq 162 13 . . . cord-290231-4m9lj0uq 163 1 A a DT cord-290231-4m9lj0uq 163 2 similar similar JJ cord-290231-4m9lj0uq 163 3 observation observation NN cord-290231-4m9lj0uq 163 4 has have VBZ cord-290231-4m9lj0uq 163 5 been be VBN cord-290231-4m9lj0uq 163 6 made make VBN cord-290231-4m9lj0uq 163 7 with with IN cord-290231-4m9lj0uq 163 8 West West NNP cord-290231-4m9lj0uq 163 9 Nile Nile NNP cord-290231-4m9lj0uq 163 10 ( ( -LRB- cord-290231-4m9lj0uq 163 11 WN WN NNP cord-290231-4m9lj0uq 163 12 ) ) -RRB- cord-290231-4m9lj0uq 163 13 virus virus NN cord-290231-4m9lj0uq 163 14 in in IN cord-290231-4m9lj0uq 163 15 which which WDT cord-290231-4m9lj0uq 163 16 the the DT cord-290231-4m9lj0uq 163 17 ratio ratio NN cord-290231-4m9lj0uq 163 18 of of IN cord-290231-4m9lj0uq 163 19 physical physical JJ cord-290231-4m9lj0uq 163 20 particles particle NNS cord-290231-4m9lj0uq 163 21 to to IN cord-290231-4m9lj0uq 163 22 PFU PFU NNP cord-290231-4m9lj0uq 163 23 was be VBD cord-290231-4m9lj0uq 163 24 about about RB cord-290231-4m9lj0uq 163 25 600 600 CD cord-290231-4m9lj0uq 163 26 for for IN cord-290231-4m9lj0uq 163 27 the the DT cord-290231-4m9lj0uq 163 28 fully fully RB cord-290231-4m9lj0uq 163 29 infectious infectious JJ cord-290231-4m9lj0uq 163 30 extracellular extracellular JJ cord-290231-4m9lj0uq 163 31 ( ( -LRB- cord-290231-4m9lj0uq 163 32 M M NNP cord-290231-4m9lj0uq 163 33 - - HYPH cord-290231-4m9lj0uq 163 34 containing contain VBG cord-290231-4m9lj0uq 163 35 ) ) -RRB- cord-290231-4m9lj0uq 163 36 viruses virus NNS cord-290231-4m9lj0uq 163 37 and and CC cord-290231-4m9lj0uq 163 38 was be VBD cord-290231-4m9lj0uq 163 39 about about RB cord-290231-4m9lj0uq 163 40 60-fold 60-fold JJ cord-290231-4m9lj0uq 163 41 higher high JJR cord-290231-4m9lj0uq 163 42 for for IN cord-290231-4m9lj0uq 163 43 the the DT cord-290231-4m9lj0uq 163 44 cell cell NN cord-290231-4m9lj0uq 163 45 - - HYPH cord-290231-4m9lj0uq 163 46 associated associate VBN cord-290231-4m9lj0uq 163 47 ( ( -LRB- cord-290231-4m9lj0uq 163 48 prM prm NN cord-290231-4m9lj0uq 163 49 - - HYPH cord-290231-4m9lj0uq 163 50 containing contain VBG cord-290231-4m9lj0uq 163 51 ) ) -RRB- cord-290231-4m9lj0uq 163 52 viruses virus NNS cord-290231-4m9lj0uq 163 53 ( ( -LRB- cord-290231-4m9lj0uq 163 54 Wengler Wengler NNP cord-290231-4m9lj0uq 163 55 and and CC cord-290231-4m9lj0uq 163 56 Wengler Wengler NNP cord-290231-4m9lj0uq 163 57 , , , cord-290231-4m9lj0uq 163 58 1989 1989 CD cord-290231-4m9lj0uq 163 59 ) ) -RRB- cord-290231-4m9lj0uq 163 60 . . . cord-290231-4m9lj0uq 164 1 Upon upon IN cord-290231-4m9lj0uq 164 2 subsequent subsequent JJ cord-290231-4m9lj0uq 164 3 infection infection NN cord-290231-4m9lj0uq 164 4 and and CC cord-290231-4m9lj0uq 164 5 endocytosis endocytosis NN cord-290231-4m9lj0uq 164 6 , , , cord-290231-4m9lj0uq 164 7 the the DT cord-290231-4m9lj0uq 164 8 E e NN cord-290231-4m9lj0uq 164 9 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 164 10 probably probably RB cord-290231-4m9lj0uq 164 11 undergoes undergo VBZ cord-290231-4m9lj0uq 164 12 a a DT cord-290231-4m9lj0uq 164 13 conformational conformational JJ cord-290231-4m9lj0uq 164 14 change change NN cord-290231-4m9lj0uq 164 15 in in IN cord-290231-4m9lj0uq 164 16 the the DT cord-290231-4m9lj0uq 164 17 acidic acidic JJ cord-290231-4m9lj0uq 164 18 environment environment NN cord-290231-4m9lj0uq 164 19 of of IN cord-290231-4m9lj0uq 164 20 endocytic endocytic JJ cord-290231-4m9lj0uq 164 21 vesicles vesicle NNS cord-290231-4m9lj0uq 164 22 , , , cord-290231-4m9lj0uq 164 23 which which WDT cord-290231-4m9lj0uq 164 24 results result VBZ cord-290231-4m9lj0uq 164 25 in in IN cord-290231-4m9lj0uq 164 26 membrane membrane NN cord-290231-4m9lj0uq 164 27 fusion fusion NN cord-290231-4m9lj0uq 164 28 and and CC cord-290231-4m9lj0uq 164 29 release release NN cord-290231-4m9lj0uq 164 30 of of IN cord-290231-4m9lj0uq 164 31 the the DT cord-290231-4m9lj0uq 164 32 nucleocapsid nucleocapsid NN cord-290231-4m9lj0uq 164 33 into into IN cord-290231-4m9lj0uq 164 34 the the DT cord-290231-4m9lj0uq 164 35 cytoplasm cytoplasm NN cord-290231-4m9lj0uq 164 36 . . . cord-290231-4m9lj0uq 165 1 Consistent consistent JJ cord-290231-4m9lj0uq 165 2 with with IN cord-290231-4m9lj0uq 165 3 this this DT cord-290231-4m9lj0uq 165 4 hypothesis hypothesis NN cord-290231-4m9lj0uq 165 5 are be VBP cord-290231-4m9lj0uq 165 6 our -PRON- PRP$ cord-290231-4m9lj0uq 165 7 previous previous JJ cord-290231-4m9lj0uq 165 8 findings finding NNS cord-290231-4m9lj0uq 165 9 that that IN cord-290231-4m9lj0uq 165 10 the the DT cord-290231-4m9lj0uq 165 11 E e NN cord-290231-4m9lj0uq 165 12 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 165 13 of of IN cord-290231-4m9lj0uq 165 14 TBE TBE NNP cord-290231-4m9lj0uq 165 15 virus virus NN cord-290231-4m9lj0uq 165 16 undergoes undergo VBZ cord-290231-4m9lj0uq 165 17 irreversible irreversible JJ cord-290231-4m9lj0uq 165 18 conformational conformational JJ cord-290231-4m9lj0uq 165 19 changes change NNS cord-290231-4m9lj0uq 165 20 at at IN cord-290231-4m9lj0uq 165 21 pH pH NNP cord-290231-4m9lj0uq 165 22 less less JJR cord-290231-4m9lj0uq 165 23 than than IN cord-290231-4m9lj0uq 165 24 6.2 6.2 CD cord-290231-4m9lj0uq 165 25 . . . cord-290231-4m9lj0uq 166 1 These these DT cord-290231-4m9lj0uq 166 2 changes change NNS cord-290231-4m9lj0uq 166 3 , , , cord-290231-4m9lj0uq 166 4 which which WDT cord-290231-4m9lj0uq 166 5 predominantly predominantly RB cord-290231-4m9lj0uq 166 6 occur occur VBP cord-290231-4m9lj0uq 166 7 in in IN cord-290231-4m9lj0uq 166 8 the the DT cord-290231-4m9lj0uq 166 9 A A NNP cord-290231-4m9lj0uq 166 10 - - HYPH cord-290231-4m9lj0uq 166 11 domain domain NN cord-290231-4m9lj0uq 166 12 epitopes epitope NNS cord-290231-4m9lj0uq 166 13 and and CC cord-290231-4m9lj0uq 166 14 the the DT cord-290231-4m9lj0uq 166 15 i2 i2 NNP cord-290231-4m9lj0uq 166 16 site site NN cord-290231-4m9lj0uq 166 17 Mandl Mandl NNP cord-290231-4m9lj0uq 166 18 et et NNP cord-290231-4m9lj0uq 166 19 al al NNP cord-290231-4m9lj0uq 166 20 . . NNP cord-290231-4m9lj0uq 166 21 , , , cord-290231-4m9lj0uq 166 22 1989 1989 CD cord-290231-4m9lj0uq 166 23 ) ) -RRB- cord-290231-4m9lj0uq 166 24 , , , cord-290231-4m9lj0uq 166 25 are be VBP cord-290231-4m9lj0uq 166 26 probably probably RB cord-290231-4m9lj0uq 166 27 necessary necessary JJ cord-290231-4m9lj0uq 166 28 to to TO cord-290231-4m9lj0uq 166 29 trigger trigger VB cord-290231-4m9lj0uq 166 30 the the DT cord-290231-4m9lj0uq 166 31 fusion fusion NN cord-290231-4m9lj0uq 166 32 activity activity NN cord-290231-4m9lj0uq 166 33 of of IN cord-290231-4m9lj0uq 166 34 the the DT cord-290231-4m9lj0uq 166 35 E e NN cord-290231-4m9lj0uq 166 36 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 166 37 . . . cord-290231-4m9lj0uq 167 1 It -PRON- PRP cord-290231-4m9lj0uq 167 2 has have VBZ cord-290231-4m9lj0uq 167 3 been be VBN cord-290231-4m9lj0uq 167 4 shown show VBN cord-290231-4m9lj0uq 167 5 that that IN cord-290231-4m9lj0uq 167 6 the the DT cord-290231-4m9lj0uq 167 7 fusion fusion NN cord-290231-4m9lj0uq 167 8 activity activity NN cord-290231-4m9lj0uq 167 9 of of IN cord-290231-4m9lj0uq 167 10 the the DT cord-290231-4m9lj0uq 167 11 TBE TBE NNP cord-290231-4m9lj0uq 167 12 virus virus NN cord-290231-4m9lj0uq 167 13 can can MD cord-290231-4m9lj0uq 167 14 be be VB cord-290231-4m9lj0uq 167 15 blocked block VBN cord-290231-4m9lj0uq 167 16 effectively effectively RB cord-290231-4m9lj0uq 167 17 by by IN cord-290231-4m9lj0uq 167 18 three three CD cord-290231-4m9lj0uq 167 19 MAbs MAbs NNP cord-290231-4m9lj0uq 167 20 , , , cord-290231-4m9lj0uq 167 21 two two CD cord-290231-4m9lj0uq 167 22 of of IN cord-290231-4m9lj0uq 167 23 which which WDT cord-290231-4m9lj0uq 167 24 were be VBD cord-290231-4m9lj0uq 167 25 specific specific JJ cord-290231-4m9lj0uq 167 26 for for IN cord-290231-4m9lj0uq 167 27 epitopes epitope NNS cord-290231-4m9lj0uq 167 28 of of IN cord-290231-4m9lj0uq 167 29 the the DT cord-290231-4m9lj0uq 167 30 A A NNP cord-290231-4m9lj0uq 167 31 - - HYPH cord-290231-4m9lj0uq 167 32 domain domain NN cord-290231-4m9lj0uq 167 33 , , , cord-290231-4m9lj0uq 167 34 and and CC cord-290231-4m9lj0uq 167 35 one one CD cord-290231-4m9lj0uq 167 36 recognized recognize VBD cord-290231-4m9lj0uq 167 37 the the DT cord-290231-4m9lj0uq 167 38 i2 i2 NNP cord-290231-4m9lj0uq 167 39 epitope epitope NN cord-290231-4m9lj0uq 167 40 ( ( -LRB- cord-290231-4m9lj0uq 167 41 Guirakhoo Guirakhoo NNP cord-290231-4m9lj0uq 167 42 et et FW cord-290231-4m9lj0uq 167 43 al al NNP cord-290231-4m9lj0uq 167 44 . . NNP cord-290231-4m9lj0uq 167 45 , , , cord-290231-4m9lj0uq 167 46 1991 1991 CD cord-290231-4m9lj0uq 167 47 ) ) -RRB- cord-290231-4m9lj0uq 167 48 . . . cord-290231-4m9lj0uq 168 1 The the DT cord-290231-4m9lj0uq 168 2 A A NNP cord-290231-4m9lj0uq 168 3 - - HYPH cord-290231-4m9lj0uq 168 4 domain domain NN cord-290231-4m9lj0uq 168 5 contains contain VBZ cord-290231-4m9lj0uq 168 6 an an DT cord-290231-4m9lj0uq 168 7 amino amino NN cord-290231-4m9lj0uq 168 8 acid acid NN cord-290231-4m9lj0uq 168 9 sequence sequence NN cord-290231-4m9lj0uq 168 10 ( ( -LRB- cord-290231-4m9lj0uq 168 11 G g NN cord-290231-4m9lj0uq 168 12 L l NN cord-290231-4m9lj0uq 168 13 F f NN cord-290231-4m9lj0uq 168 14 G g NN cord-290231-4m9lj0uq 168 15 ) ) -RRB- cord-290231-4m9lj0uq 168 16 that that WDT cord-290231-4m9lj0uq 168 17 is be VBZ cord-290231-4m9lj0uq 168 18 conserved conserve VBN cord-290231-4m9lj0uq 168 19 in in IN cord-290231-4m9lj0uq 168 20 the the DT cord-290231-4m9lj0uq 168 21 fusion fusion NN cord-290231-4m9lj0uq 168 22 sequences sequence NNS cord-290231-4m9lj0uq 168 23 of of IN cord-290231-4m9lj0uq 168 24 other other JJ cord-290231-4m9lj0uq 168 25 enveloped envelop VBN cord-290231-4m9lj0uq 168 26 viruses virus NNS cord-290231-4m9lj0uq 168 27 . . . cord-290231-4m9lj0uq 169 1 We -PRON- PRP cord-290231-4m9lj0uq 169 2 have have VBP cord-290231-4m9lj0uq 169 3 also also RB cord-290231-4m9lj0uq 169 4 observed observe VBN cord-290231-4m9lj0uq 169 5 with with IN cord-290231-4m9lj0uq 169 6 dengue dengue NN cord-290231-4m9lj0uq 169 7 virus virus NN cord-290231-4m9lj0uq 169 8 that that WDT cord-290231-4m9lj0uq 169 9 the the DT cord-290231-4m9lj0uq 169 10 region region NN cord-290231-4m9lj0uq 169 11 containing contain VBG cord-290231-4m9lj0uq 169 12 the the DT cord-290231-4m9lj0uq 169 13 conservative conservative JJ cord-290231-4m9lj0uq 169 14 fusion fusion NN cord-290231-4m9lj0uq 169 15 sequence sequence NN cord-290231-4m9lj0uq 169 16 is be VBZ cord-290231-4m9lj0uq 169 17 more more RBR cord-290231-4m9lj0uq 169 18 accessible accessible JJ cord-290231-4m9lj0uq 169 19 to to IN cord-290231-4m9lj0uq 169 20 antipeptide antipeptide NNP cord-290231-4m9lj0uq 169 21 antibodies antibody NNS cord-290231-4m9lj0uq 169 22 following follow VBG cord-290231-4m9lj0uq 169 23 low low JJ cord-290231-4m9lj0uq 169 24 pH pH NNP cord-290231-4m9lj0uq 169 25 treatment treatment NN cord-290231-4m9lj0uq 169 26 . . . cord-290231-4m9lj0uq 170 1 By by IN cord-290231-4m9lj0uq 170 2 using use VBG cord-290231-4m9lj0uq 170 3 MAbs MAbs NNP cord-290231-4m9lj0uq 170 4 specific specific JJ cord-290231-4m9lj0uq 170 5 for for IN cord-290231-4m9lj0uq 170 6 MVE MVE NNP cord-290231-4m9lj0uq 170 7 virus virus NN cord-290231-4m9lj0uq 170 8 E e NN cord-290231-4m9lj0uq 170 9 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 170 10 in in IN cord-290231-4m9lj0uq 170 11 a a DT cord-290231-4m9lj0uq 170 12 capture capture NN cord-290231-4m9lj0uq 170 13 ELISA ELISA NNP cord-290231-4m9lj0uq 170 14 , , , cord-290231-4m9lj0uq 170 15 we -PRON- PRP cord-290231-4m9lj0uq 170 16 demonstrated demonstrate VBD cord-290231-4m9lj0uq 170 17 that that IN cord-290231-4m9lj0uq 170 18 if if IN cord-290231-4m9lj0uq 170 19 the the DT cord-290231-4m9lj0uq 170 20 processing processing NN cord-290231-4m9lj0uq 170 21 of of IN cord-290231-4m9lj0uq 170 22 prM prm NN cord-290231-4m9lj0uq 170 23 was be VBD cord-290231-4m9lj0uq 170 24 interrupted interrupt VBN cord-290231-4m9lj0uq 170 25 at at RB cord-290231-4m9lj0uq 170 26 least least RBS cord-290231-4m9lj0uq 170 27 three three CD cord-290231-4m9lj0uq 170 28 of of IN cord-290231-4m9lj0uq 170 29 these these DT cord-290231-4m9lj0uq 170 30 epitopes epitope NNS cord-290231-4m9lj0uq 170 31 on on IN cord-290231-4m9lj0uq 170 32 the the DT cord-290231-4m9lj0uq 170 33 E e NN cord-290231-4m9lj0uq 170 34 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 170 35 were be VBD cord-290231-4m9lj0uq 170 36 less less RBR cord-290231-4m9lj0uq 170 37 accessible accessible JJ cord-290231-4m9lj0uq 170 38 on on IN cord-290231-4m9lj0uq 170 39 the the DT cord-290231-4m9lj0uq 170 40 virus virus NN cord-290231-4m9lj0uq 170 41 particle particle NN cord-290231-4m9lj0uq 170 42 . . . cord-290231-4m9lj0uq 171 1 The the DT cord-290231-4m9lj0uq 171 2 observation observation NN cord-290231-4m9lj0uq 171 3 that that IN cord-290231-4m9lj0uq 171 4 all all DT cord-290231-4m9lj0uq 171 5 of of IN cord-290231-4m9lj0uq 171 6 the the DT cord-290231-4m9lj0uq 171 7 epitopes epitope NNS cord-290231-4m9lj0uq 171 8 were be VBD cord-290231-4m9lj0uq 171 9 acid acid JJ cord-290231-4m9lj0uq 171 10 sensitive sensitive JJ cord-290231-4m9lj0uq 171 11 indicates indicate VBZ cord-290231-4m9lj0uq 171 12 that that IN cord-290231-4m9lj0uq 171 13 they -PRON- PRP cord-290231-4m9lj0uq 171 14 are be VBP cord-290231-4m9lj0uq 171 15 probably probably RB cord-290231-4m9lj0uq 171 16 located locate VBN cord-290231-4m9lj0uq 171 17 in in IN cord-290231-4m9lj0uq 171 18 the the DT cord-290231-4m9lj0uq 171 19 A A NNP cord-290231-4m9lj0uq 171 20 - - HYPH cord-290231-4m9lj0uq 171 21 domain domain NN cord-290231-4m9lj0uq 171 22 . . . cord-290231-4m9lj0uq 172 1 The the DT cord-290231-4m9lj0uq 172 2 sensitivity sensitivity NN cord-290231-4m9lj0uq 172 3 of of IN cord-290231-4m9lj0uq 172 4 the the DT cord-290231-4m9lj0uq 172 5 HIAF HIAF NNPS cord-290231-4m9lj0uq 172 6 to to IN cord-290231-4m9lj0uq 172 7 low low JJ cord-290231-4m9lj0uq 172 8 pH ph NN cord-290231-4m9lj0uq 172 9 also also RB cord-290231-4m9lj0uq 172 10 suggests suggest VBZ cord-290231-4m9lj0uq 172 11 that that IN cord-290231-4m9lj0uq 172 12 these these DT cord-290231-4m9lj0uq 172 13 epitopes epitope NNS cord-290231-4m9lj0uq 172 14 are be VBP cord-290231-4m9lj0uq 172 15 the the DT cord-290231-4m9lj0uq 172 16 predominant predominant JJ cord-290231-4m9lj0uq 172 17 epitopes epitope NNS cord-290231-4m9lj0uq 172 18 recognized recognize VBD cord-290231-4m9lj0uq 172 19 following follow VBG cord-290231-4m9lj0uq 172 20 immunization immunization NN cord-290231-4m9lj0uq 172 21 with with IN cord-290231-4m9lj0uq 172 22 intact intact JJ cord-290231-4m9lj0uq 172 23 virus virus NN cord-290231-4m9lj0uq 172 24 . . . cord-290231-4m9lj0uq 173 1 It -PRON- PRP cord-290231-4m9lj0uq 173 2 may may MD cord-290231-4m9lj0uq 173 3 be be VB cord-290231-4m9lj0uq 173 4 significant significant JJ cord-290231-4m9lj0uq 173 5 that that IN cord-290231-4m9lj0uq 173 6 these these DT cord-290231-4m9lj0uq 173 7 three three CD cord-290231-4m9lj0uq 173 8 epitopes epitope NNS cord-290231-4m9lj0uq 173 9 are be VBP cord-290231-4m9lj0uq 173 10 associated associate VBN cord-290231-4m9lj0uq 173 11 with with IN cord-290231-4m9lj0uq 173 12 hemagglutination hemagglutination NN cord-290231-4m9lj0uq 173 13 of of IN cord-290231-4m9lj0uq 173 14 red red JJ cord-290231-4m9lj0uq 173 15 blood blood NN cord-290231-4m9lj0uq 173 16 cells cell NNS cord-290231-4m9lj0uq 173 17 . . . cord-290231-4m9lj0uq 174 1 At at RB cord-290231-4m9lj0uq 174 2 least least RBS cord-290231-4m9lj0uq 174 3 one one CD cord-290231-4m9lj0uq 174 4 of of IN cord-290231-4m9lj0uq 174 5 these these DT cord-290231-4m9lj0uq 174 6 epitopes epitope NNS cord-290231-4m9lj0uq 174 7 ( ( -LRB- cord-290231-4m9lj0uq 174 8 E-8 E-8 NNP cord-290231-4m9lj0uq 174 9 ) ) -RRB- cord-290231-4m9lj0uq 174 10 also also RB cord-290231-4m9lj0uq 174 11 elicits elicit VBZ cord-290231-4m9lj0uq 174 12 virus virus NN cord-290231-4m9lj0uq 174 13 - - HYPH cord-290231-4m9lj0uq 174 14 neutralizing neutralize VBG cord-290231-4m9lj0uq 174 15 antibodies antibody NNS cord-290231-4m9lj0uq 174 16 . . . cord-290231-4m9lj0uq 175 1 Since since IN cord-290231-4m9lj0uq 175 2 other other JJ cord-290231-4m9lj0uq 175 3 functionally functionally RB cord-290231-4m9lj0uq 175 4 important important JJ cord-290231-4m9lj0uq 175 5 epitopes epitope NNS cord-290231-4m9lj0uq 175 6 which which WDT cord-290231-4m9lj0uq 175 7 induce induce VBP cord-290231-4m9lj0uq 175 8 neutralizing neutralize VBG cord-290231-4m9lj0uq 175 9 , , , cord-290231-4m9lj0uq 175 10 hemagglutinationinhibiting hemagglutinationinhibite VBG cord-290231-4m9lj0uq 175 11 , , , cord-290231-4m9lj0uq 175 12 and and CC cord-290231-4m9lj0uq 175 13 protective protective JJ cord-290231-4m9lj0uq 175 14 antibodies antibody NNS cord-290231-4m9lj0uq 175 15 ( ( -LRB- cord-290231-4m9lj0uq 175 16 Hawkes Hawkes NNP cord-290231-4m9lj0uq 175 17 et et NNP cord-290231-4m9lj0uq 175 18 a/. a/. NNP cord-290231-4m9lj0uq 175 19 , , , cord-290231-4m9lj0uq 175 20 1988 1988 CD cord-290231-4m9lj0uq 175 21 ) ) -RRB- cord-290231-4m9lj0uq 175 22 are be VBP cord-290231-4m9lj0uq 175 23 still still RB cord-290231-4m9lj0uq 175 24 present present JJ cord-290231-4m9lj0uq 175 25 in in IN cord-290231-4m9lj0uq 175 26 the the DT cord-290231-4m9lj0uq 175 27 prM prm NN cord-290231-4m9lj0uq 175 28 - - HYPH cord-290231-4m9lj0uq 175 29 containing contain VBG cord-290231-4m9lj0uq 175 30 viruses virus NNS cord-290231-4m9lj0uq 176 1 ( ( -LRB- cord-290231-4m9lj0uq 176 2 Fig Fig NNP cord-290231-4m9lj0uq 176 3 . . . cord-290231-4m9lj0uq 176 4 2 2 CD cord-290231-4m9lj0uq 176 5 A a CD cord-290231-4m9lj0uq 176 6 ) ) -RRB- cord-290231-4m9lj0uq 176 7 , , , cord-290231-4m9lj0uq 176 8 and and CC cord-290231-4m9lj0uq 176 9 the the DT cord-290231-4m9lj0uq 176 10 fact fact NN cord-290231-4m9lj0uq 176 11 that that IN cord-290231-4m9lj0uq 176 12 these these DT cord-290231-4m9lj0uq 176 13 viruses virus NNS cord-290231-4m9lj0uq 176 14 are be VBP cord-290231-4m9lj0uq 176 15 still still RB cord-290231-4m9lj0uq 176 16 capable capable JJ cord-290231-4m9lj0uq 176 17 of of IN cord-290231-4m9lj0uq 176 18 binding bind VBG cord-290231-4m9lj0uq 176 19 to to IN cord-290231-4m9lj0uq 176 20 and and CC cord-290231-4m9lj0uq 176 21 infecting infect VBG cord-290231-4m9lj0uq 176 22 their -PRON- PRP$ cord-290231-4m9lj0uq 176 23 host host NN cord-290231-4m9lj0uq 176 24 cells cell NNS cord-290231-4m9lj0uq 176 25 , , , cord-290231-4m9lj0uq 176 26 we -PRON- PRP cord-290231-4m9lj0uq 176 27 did do VBD cord-290231-4m9lj0uq 176 28 not not RB cord-290231-4m9lj0uq 176 29 expect expect VB cord-290231-4m9lj0uq 176 30 that that IN cord-290231-4m9lj0uq 176 31 the the DT cord-290231-4m9lj0uq 176 32 fusion fusion NN cord-290231-4m9lj0uq 176 33 peptide peptide NN cord-290231-4m9lj0uq 176 34 or or CC cord-290231-4m9lj0uq 176 35 the the DT cord-290231-4m9lj0uq 176 36 receptorbinding receptorbinding NN cord-290231-4m9lj0uq 176 37 sites site NNS cord-290231-4m9lj0uq 176 38 would would MD cord-290231-4m9lj0uq 176 39 be be VB cord-290231-4m9lj0uq 176 40 directly directly RB cord-290231-4m9lj0uq 176 41 involved involve VBN cord-290231-4m9lj0uq 176 42 in in IN cord-290231-4m9lj0uq 176 43 the the DT cord-290231-4m9lj0uq 176 44 prM prm JJ cord-290231-4m9lj0uq 176 45 - - HYPH cord-290231-4m9lj0uq 176 46 E e NN cord-290231-4m9lj0uq 176 47 interactions interaction NNS cord-290231-4m9lj0uq 176 48 . . . cord-290231-4m9lj0uq 177 1 This this DT cord-290231-4m9lj0uq 177 2 was be VBD cord-290231-4m9lj0uq 177 3 confirmed confirm VBN cord-290231-4m9lj0uq 177 4 when when WRB cord-290231-4m9lj0uq 177 5 the the DT cord-290231-4m9lj0uq 177 6 immunereactive immunereactive JJ cord-290231-4m9lj0uq 177 7 fragments fragment NNS cord-290231-4m9lj0uq 177 8 generated generate VBN cord-290231-4m9lj0uq 177 9 by by IN cord-290231-4m9lj0uq 177 10 V8 v8 NN cord-290231-4m9lj0uq 177 11 protease protease NN cord-290231-4m9lj0uq 177 12 were be VBD cord-290231-4m9lj0uq 177 13 sequenced sequence VBN cord-290231-4m9lj0uq 177 14 . . . cord-290231-4m9lj0uq 178 1 It -PRON- PRP cord-290231-4m9lj0uq 178 2 was be VBD cord-290231-4m9lj0uq 178 3 shown show VBN cord-290231-4m9lj0uq 178 4 that that IN cord-290231-4m9lj0uq 178 5 predominantly predominantly RB cord-290231-4m9lj0uq 178 6 three three CD cord-290231-4m9lj0uq 178 7 sites site NNS cord-290231-4m9lj0uq 178 8 on on IN cord-290231-4m9lj0uq 178 9 the the DT cord-290231-4m9lj0uq 178 10 E e NN cord-290231-4m9lj0uq 178 11 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 178 12 were be VBD cord-290231-4m9lj0uq 178 13 exposed expose VBN cord-290231-4m9lj0uq 178 14 to to IN cord-290231-4m9lj0uq 178 15 the the DT cord-290231-4m9lj0uq 178 16 proteolytic proteolytic JJ cord-290231-4m9lj0uq 178 17 attack attack NN cord-290231-4m9lj0uq 178 18 of of IN cord-290231-4m9lj0uq 178 19 the the DT cord-290231-4m9lj0uq 178 20 V8 V8 NNP cord-290231-4m9lj0uq 178 21 enzyme enzyme NN cord-290231-4m9lj0uq 178 22 . . . cord-290231-4m9lj0uq 179 1 This this DT cord-290231-4m9lj0uq 179 2 cleavage cleavage NN cord-290231-4m9lj0uq 179 3 , , , cord-290231-4m9lj0uq 179 4 which which WDT cord-290231-4m9lj0uq 179 5 occurred occur VBD cord-290231-4m9lj0uq 179 6 on on IN cord-290231-4m9lj0uq 179 7 positions position NNS cord-290231-4m9lj0uq 179 8 138 138 CD cord-290231-4m9lj0uq 179 9 , , , cord-290231-4m9lj0uq 179 10 199 199 CD cord-290231-4m9lj0uq 179 11 , , , cord-290231-4m9lj0uq 179 12 and and CC cord-290231-4m9lj0uq 179 13 326 326 CD cord-290231-4m9lj0uq 179 14 , , , cord-290231-4m9lj0uq 179 15 generated generate VBD cord-290231-4m9lj0uq 179 16 four four CD cord-290231-4m9lj0uq 179 17 fragments fragment NNS cord-290231-4m9lj0uq 179 18 reactive reactive JJ cord-290231-4m9lj0uq 179 19 with with IN cord-290231-4m9lj0uq 179 20 sequence sequence NN cord-290231-4m9lj0uq 179 21 - - HYPH cord-290231-4m9lj0uq 179 22 specific specific JJ cord-290231-4m9lj0uq 179 23 antipeptide antipeptide JJ cord-290231-4m9lj0uq 179 24 antibody antibody NN cord-290231-4m9lj0uq 179 25 or or CC cord-290231-4m9lj0uq 179 26 E-8 E-8 NNP cord-290231-4m9lj0uq 179 27 MAb MAb NNS cord-290231-4m9lj0uq 179 28 . . . cord-290231-4m9lj0uq 180 1 Similar similar JJ cord-290231-4m9lj0uq 180 2 segments segment NNS cord-290231-4m9lj0uq 180 3 on on IN cord-290231-4m9lj0uq 180 4 the the DT cord-290231-4m9lj0uq 180 5 E e NN cord-290231-4m9lj0uq 180 6 pro pro FW cord-290231-4m9lj0uq 180 7 - - JJ cord-290231-4m9lj0uq 180 8 tein tein NNP cord-290231-4m9lj0uq 180 9 of of IN cord-290231-4m9lj0uq 180 10 WN WN NNP cord-290231-4m9lj0uq 180 11 virus virus NN cord-290231-4m9lj0uq 180 12 , , , cord-290231-4m9lj0uq 180 13 which which WDT cord-290231-4m9lj0uq 180 14 are be VBP cord-290231-4m9lj0uq 180 15 located locate VBN cord-290231-4m9lj0uq 180 16 in in IN cord-290231-4m9lj0uq 180 17 Ll Ll NNP cord-290231-4m9lj0uq 180 18 and and CC cord-290231-4m9lj0uq 180 19 R3 R3 NNP cord-290231-4m9lj0uq 180 20 , , , cord-290231-4m9lj0uq 180 21 have have VBP cord-290231-4m9lj0uq 180 22 also also RB cord-290231-4m9lj0uq 180 23 been be VBN cord-290231-4m9lj0uq 180 24 shown show VBN cord-290231-4m9lj0uq 180 25 to to TO cord-290231-4m9lj0uq 180 26 be be VB cord-290231-4m9lj0uq 180 27 exposed expose VBN cord-290231-4m9lj0uq 180 28 to to IN cord-290231-4m9lj0uq 180 29 proteolytic proteolytic JJ cord-290231-4m9lj0uq 180 30 attack attack NN cord-290231-4m9lj0uq 180 31 ( ( -LRB- cord-290231-4m9lj0uq 180 32 Wengler Wengler NNP cord-290231-4m9lj0uq 180 33 er er UH cord-290231-4m9lj0uq 180 34 a/. a/. NNP cord-290231-4m9lj0uq 180 35 , , , cord-290231-4m9lj0uq 180 36 1987 1987 CD cord-290231-4m9lj0uq 180 37 ) ) -RRB- cord-290231-4m9lj0uq 180 38 . . . cord-290231-4m9lj0uq 181 1 Combining combine VBG cord-290231-4m9lj0uq 181 2 results result NNS cord-290231-4m9lj0uq 181 3 of of IN cord-290231-4m9lj0uq 181 4 the the DT cord-290231-4m9lj0uq 181 5 reactivity reactivity NN cord-290231-4m9lj0uq 181 6 pattern pattern NN cord-290231-4m9lj0uq 181 7 of of IN cord-290231-4m9lj0uq 181 8 the the DT cord-290231-4m9lj0uq 181 9 antipeptide antipeptide JJ cord-290231-4m9lj0uq 181 10 antibodies antibody NNS cord-290231-4m9lj0uq 181 11 with with IN cord-290231-4m9lj0uq 181 12 proteolytic proteolytic JJ cord-290231-4m9lj0uq 181 13 fragments fragment NNS cord-290231-4m9lj0uq 181 14 and and CC cord-290231-4m9lj0uq 181 15 also also RB cord-290231-4m9lj0uq 181 16 information information NN cord-290231-4m9lj0uq 181 17 obtained obtain VBN cord-290231-4m9lj0uq 181 18 from from IN cord-290231-4m9lj0uq 181 19 N n CD cord-290231-4m9lj0uq 181 20 - - HYPH cord-290231-4m9lj0uq 181 21 terminal terminal JJ cord-290231-4m9lj0uq 181 22 sequence sequence NN cord-290231-4m9lj0uq 181 23 analysis analysis NN cord-290231-4m9lj0uq 181 24 of of IN cord-290231-4m9lj0uq 181 25 the the DT cord-290231-4m9lj0uq 181 26 immune immune JJ cord-290231-4m9lj0uq 181 27 fragments fragment NNS cord-290231-4m9lj0uq 181 28 , , , cord-290231-4m9lj0uq 181 29 we -PRON- PRP cord-290231-4m9lj0uq 181 30 were be VBD cord-290231-4m9lj0uq 181 31 able able JJ cord-290231-4m9lj0uq 181 32 to to TO cord-290231-4m9lj0uq 181 33 map map VB cord-290231-4m9lj0uq 181 34 the the DT cord-290231-4m9lj0uq 181 35 binding binding NN cord-290231-4m9lj0uq 181 36 site site NN cord-290231-4m9lj0uq 181 37 of of IN cord-290231-4m9lj0uq 181 38 the the DT cord-290231-4m9lj0uq 181 39 E-8 E-8 NNP cord-290231-4m9lj0uq 181 40 MAb MAb NNS cord-290231-4m9lj0uq 181 41 which which WDT cord-290231-4m9lj0uq 181 42 was be VBD cord-290231-4m9lj0uq 181 43 covered cover VBN cord-290231-4m9lj0uq 181 44 or or CC cord-290231-4m9lj0uq 181 45 dissociated dissociate VBN cord-290231-4m9lj0uq 181 46 by by IN cord-290231-4m9lj0uq 181 47 the the DT cord-290231-4m9lj0uq 181 48 presence presence NN cord-290231-4m9lj0uq 181 49 of of IN cord-290231-4m9lj0uq 181 50 prM. prm. XX cord-290231-4m9lj0uq 182 1 This this DT cord-290231-4m9lj0uq 182 2 site site NN cord-290231-4m9lj0uq 182 3 which which WDT cord-290231-4m9lj0uq 182 4 reside reside VBP cord-290231-4m9lj0uq 182 5 between between IN cord-290231-4m9lj0uq 182 6 amino amino NN cord-290231-4m9lj0uq 182 7 acids acid NNS cord-290231-4m9lj0uq 182 8 200 200 CD cord-290231-4m9lj0uq 182 9 and and CC cord-290231-4m9lj0uq 182 10 327 327 CD cord-290231-4m9lj0uq 182 11 on on IN cord-290231-4m9lj0uq 182 12 the the DT cord-290231-4m9lj0uq 182 13 E e NN cord-290231-4m9lj0uq 182 14 protein protein NN cord-290231-4m9lj0uq 182 15 includes include VBZ cord-290231-4m9lj0uq 182 16 more more JJR cord-290231-4m9lj0uq 182 17 than than IN cord-290231-4m9lj0uq 182 18 80 80 CD cord-290231-4m9lj0uq 182 19 % % NN cord-290231-4m9lj0uq 182 20 of of IN cord-290231-4m9lj0uq 182 21 the the DT cord-290231-4m9lj0uq 182 22 R2 R2 NNP cord-290231-4m9lj0uq 182 23 domain domain NN cord-290231-4m9lj0uq 182 24 proposed propose VBN cord-290231-4m9lj0uq 182 25 for for IN cord-290231-4m9lj0uq 182 26 WN WN NNP cord-290231-4m9lj0uq 182 27 ( ( -LRB- cord-290231-4m9lj0uq 182 28 Nowak Nowak NNP cord-290231-4m9lj0uq 182 29 and and CC cord-290231-4m9lj0uq 182 30 Wengier Wengier NNP cord-290231-4m9lj0uq 182 31 , , , cord-290231-4m9lj0uq 182 32 1987 1987 CD cord-290231-4m9lj0uq 182 33 ) ) -RRB- cord-290231-4m9lj0uq 182 34 and and CC cord-290231-4m9lj0uq 182 35 MVE MVE NNP cord-290231-4m9lj0uq 182 36 ( ( -LRB- cord-290231-4m9lj0uq 182 37 Roehrig Roehrig NNP cord-290231-4m9lj0uq 182 38 et et FW cord-290231-4m9lj0uq 182 39 a/. a/. NN cord-290231-4m9lj0uq 182 40 , , , cord-290231-4m9lj0uq 182 41 1989 1989 CD cord-290231-4m9lj0uq 182 42 ) ) -RRB- cord-290231-4m9lj0uq 182 43 viruses virus NNS cord-290231-4m9lj0uq 182 44 . . . cord-290231-4m9lj0uq 183 1 No no DT cord-290231-4m9lj0uq 183 2 actual actual JJ cord-290231-4m9lj0uq 183 3 biological biological JJ cord-290231-4m9lj0uq 183 4 function function NN cord-290231-4m9lj0uq 183 5 of of IN cord-290231-4m9lj0uq 183 6 the the DT cord-290231-4m9lj0uq 183 7 R2 R2 NNP cord-290231-4m9lj0uq 183 8 domain domain NN cord-290231-4m9lj0uq 183 9 had have VBD cord-290231-4m9lj0uq 183 10 been be VBN cord-290231-4m9lj0uq 183 11 previously previously RB cord-290231-4m9lj0uq 183 12 identified identify VBN cord-290231-4m9lj0uq 183 13 . . . cord-290231-4m9lj0uq 184 1 It -PRON- PRP cord-290231-4m9lj0uq 184 2 is be VBZ cord-290231-4m9lj0uq 184 3 noteworthy noteworthy JJ cord-290231-4m9lj0uq 184 4 that that IN cord-290231-4m9lj0uq 184 5 the the DT cord-290231-4m9lj0uq 184 6 synthetic synthetic JJ cord-290231-4m9lj0uq 184 7 peptide peptide NN cord-290231-4m9lj0uq 184 8 MVE MVE NNP cord-290231-4m9lj0uq 184 9 06 06 CD cord-290231-4m9lj0uq 184 10 , , , cord-290231-4m9lj0uq 184 11 which which WDT cord-290231-4m9lj0uq 184 12 consists consist VBZ cord-290231-4m9lj0uq 184 13 of of IN cord-290231-4m9lj0uq 184 14 21 21 CD cord-290231-4m9lj0uq 184 15 amino amino JJ cord-290231-4m9lj0uq 184 16 acids acid NNS cord-290231-4m9lj0uq 184 17 within within IN cord-290231-4m9lj0uq 184 18 the the DT cord-290231-4m9lj0uq 184 19 R2 R2 NNP cord-290231-4m9lj0uq 184 20 domain domain NN cord-290231-4m9lj0uq 184 21 ( ( -LRB- cord-290231-4m9lj0uq 184 22 230 230 CD cord-290231-4m9lj0uq 184 23 - - SYM cord-290231-4m9lj0uq 184 24 251 251 CD cord-290231-4m9lj0uq 184 25 ) ) -RRB- cord-290231-4m9lj0uq 184 26 , , , cord-290231-4m9lj0uq 184 27 was be VBD cord-290231-4m9lj0uq 184 28 cross cross JJ cord-290231-4m9lj0uq 184 29 - - JJ cord-290231-4m9lj0uq 184 30 reactive reactive JJ cord-290231-4m9lj0uq 184 31 with with IN cord-290231-4m9lj0uq 184 32 other other JJ cord-290231-4m9lj0uq 184 33 members member NNS cord-290231-4m9lj0uq 184 34 of of IN cord-290231-4m9lj0uq 184 35 the the DT cord-290231-4m9lj0uq 184 36 flaviviruses flavivirus NNS cord-290231-4m9lj0uq 185 1 ( ( -LRB- cord-290231-4m9lj0uq 185 2 Roehrig Roehrig NNP cord-290231-4m9lj0uq 185 3 et et FW cord-290231-4m9lj0uq 185 4 al al NNP cord-290231-4m9lj0uq 185 5 . . NNP cord-290231-4m9lj0uq 185 6 , , , cord-290231-4m9lj0uq 185 7 1989 1989 CD cord-290231-4m9lj0uq 185 8 ) ) -RRB- cord-290231-4m9lj0uq 185 9 , , , cord-290231-4m9lj0uq 185 10 indicating indicate VBG cord-290231-4m9lj0uq 185 11 the the DT cord-290231-4m9lj0uq 185 12 biological biological JJ cord-290231-4m9lj0uq 185 13 importance importance NN cord-290231-4m9lj0uq 185 14 of of IN cord-290231-4m9lj0uq 185 15 this this DT cord-290231-4m9lj0uq 185 16 region region NN cord-290231-4m9lj0uq 185 17 . . . cord-290231-4m9lj0uq 186 1 Several several JJ cord-290231-4m9lj0uq 186 2 studies study NNS cord-290231-4m9lj0uq 186 3 have have VBP cord-290231-4m9lj0uq 186 4 indicated indicate VBN cord-290231-4m9lj0uq 186 5 that that IN cord-290231-4m9lj0uq 186 6 when when WRB cord-290231-4m9lj0uq 186 7 influenza influenza NN cord-290231-4m9lj0uq 186 8 A a DT cord-290231-4m9lj0uq 186 9 virus virus NN cord-290231-4m9lj0uq 186 10 and and CC cord-290231-4m9lj0uq 186 11 Semliki Semliki NNP cord-290231-4m9lj0uq 186 12 Forest Forest NNP cord-290231-4m9lj0uq 186 13 virus virus NN cord-290231-4m9lj0uq 186 14 were be VBD cord-290231-4m9lj0uq 186 15 incubated incubate VBN cord-290231-4m9lj0uq 186 16 at at IN cord-290231-4m9lj0uq 186 17 low low JJ cord-290231-4m9lj0uq 186 18 pH pH NNP cord-290231-4m9lj0uq 186 19 they -PRON- PRP cord-290231-4m9lj0uq 186 20 became become VBD cord-290231-4m9lj0uq 186 21 irreversibly irreversibly RB cord-290231-4m9lj0uq 186 22 inactivated inactivate VBN cord-290231-4m9lj0uq 186 23 ( ( -LRB- cord-290231-4m9lj0uq 186 24 Doms Doms NNP cord-290231-4m9lj0uq 186 25 et et NNP cord-290231-4m9lj0uq 186 26 a/. a/. NN cord-290231-4m9lj0uq 186 27 , , , cord-290231-4m9lj0uq 186 28 1985 1985 CD cord-290231-4m9lj0uq 186 29 ; ; : cord-290231-4m9lj0uq 186 30 Kielian Kielian NNP cord-290231-4m9lj0uq 186 31 and and CC cord-290231-4m9lj0uq 186 32 Helenius Helenius NNP cord-290231-4m9lj0uq 186 33 1985 1985 CD cord-290231-4m9lj0uq 186 34 ; ; : cord-290231-4m9lj0uq 186 35 Stegman Stegman NNP cord-290231-4m9lj0uq 186 36 eta/. eta/. NNP cord-290231-4m9lj0uq 186 37 , , , cord-290231-4m9lj0uq 186 38 1989 1989 CD cord-290231-4m9lj0uq 186 39 ; ; : cord-290231-4m9lj0uq 186 40 White White NNP cord-290231-4m9lj0uq 186 41 et et NNP cord-290231-4m9lj0uq 186 42 a/. a/. NNP cord-290231-4m9lj0uq 186 43 , , , cord-290231-4m9lj0uq 186 44 1982 1982 CD cord-290231-4m9lj0uq 186 45 ) ) -RRB- cord-290231-4m9lj0uq 186 46 . . . cord-290231-4m9lj0uq 187 1 This this DT cord-290231-4m9lj0uq 187 2 is be VBZ cord-290231-4m9lj0uq 187 3 probably probably RB cord-290231-4m9lj0uq 187 4 due due JJ cord-290231-4m9lj0uq 187 5 to to IN cord-290231-4m9lj0uq 187 6 exposure exposure NN cord-290231-4m9lj0uq 187 7 and and CC cord-290231-4m9lj0uq 187 8 self self NN cord-290231-4m9lj0uq 187 9 - - HYPH cord-290231-4m9lj0uq 187 10 aggregation aggregation NN cord-290231-4m9lj0uq 187 11 of of IN cord-290231-4m9lj0uq 187 12 the the DT cord-290231-4m9lj0uq 187 13 hydrophobic hydrophobic JJ cord-290231-4m9lj0uq 187 14 fusion fusion NN cord-290231-4m9lj0uq 187 15 peptide peptide NN cord-290231-4m9lj0uq 187 16 which which WDT cord-290231-4m9lj0uq 187 17 , , , cord-290231-4m9lj0uq 187 18 therefore therefore RB cord-290231-4m9lj0uq 187 19 , , , cord-290231-4m9lj0uq 187 20 can can MD cord-290231-4m9lj0uq 187 21 no no RB cord-290231-4m9lj0uq 187 22 longer longer RB cord-290231-4m9lj0uq 187 23 interact interact VB cord-290231-4m9lj0uq 187 24 with with IN cord-290231-4m9lj0uq 187 25 target target NN cord-290231-4m9lj0uq 187 26 membranes membrane NNS cord-290231-4m9lj0uq 187 27 ( ( -LRB- cord-290231-4m9lj0uq 187 28 Nir Nir NNP cord-290231-4m9lj0uq 187 29 et et NNP cord-290231-4m9lj0uq 187 30 al al NNP cord-290231-4m9lj0uq 187 31 . . NNP cord-290231-4m9lj0uq 187 32 , , , cord-290231-4m9lj0uq 187 33 1990 1990 CD cord-290231-4m9lj0uq 187 34 ) ) -RRB- cord-290231-4m9lj0uq 187 35 . . . cord-290231-4m9lj0uq 188 1 If if IN cord-290231-4m9lj0uq 188 2 the the DT cord-290231-4m9lj0uq 188 3 flavivirus flavivirus JJ cord-290231-4m9lj0uq 188 4 E e NN cord-290231-4m9lj0uq 188 5 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 188 6 were be VBD cord-290231-4m9lj0uq 188 7 not not RB cord-290231-4m9lj0uq 188 8 protected protect VBN cord-290231-4m9lj0uq 188 9 during during IN cord-290231-4m9lj0uq 188 10 transport transport NN cord-290231-4m9lj0uq 188 11 to to IN cord-290231-4m9lj0uq 188 12 the the DT cord-290231-4m9lj0uq 188 13 cell cell NN cord-290231-4m9lj0uq 188 14 surface surface NN cord-290231-4m9lj0uq 188 15 , , , cord-290231-4m9lj0uq 188 16 the the DT cord-290231-4m9lj0uq 188 17 acidic acidic JJ cord-290231-4m9lj0uq 188 18 milieu milieu NN cord-290231-4m9lj0uq 188 19 of of IN cord-290231-4m9lj0uq 188 20 the the DT cord-290231-4m9lj0uq 188 21 post post JJ cord-290231-4m9lj0uq 188 22 - - JJ cord-290231-4m9lj0uq 188 23 Golgi golgi JJ cord-290231-4m9lj0uq 188 24 vesicles vesicle NNS cord-290231-4m9lj0uq 188 25 could could MD cord-290231-4m9lj0uq 188 26 inactivate inactivate VB cord-290231-4m9lj0uq 188 27 it -PRON- PRP cord-290231-4m9lj0uq 188 28 . . . cord-290231-4m9lj0uq 189 1 Because because IN cord-290231-4m9lj0uq 189 2 it -PRON- PRP cord-290231-4m9lj0uq 189 3 has have VBZ cord-290231-4m9lj0uq 189 4 been be VBN cord-290231-4m9lj0uq 189 5 shown show VBN cord-290231-4m9lj0uq 189 6 with with IN cord-290231-4m9lj0uq 189 7 WN WN NNP cord-290231-4m9lj0uq 189 8 virus virus NN cord-290231-4m9lj0uq 189 9 that that WDT cord-290231-4m9lj0uq 189 10 the the DT cord-290231-4m9lj0uq 189 11 E e NN cord-290231-4m9lj0uq 189 12 and and CC cord-290231-4m9lj0uq 189 13 prM prm JJ cord-290231-4m9lj0uq 189 14 proteins protein NNS cord-290231-4m9lj0uq 189 15 remain remain VBP cord-290231-4m9lj0uq 189 16 as as IN cord-290231-4m9lj0uq 189 17 heterodimers heterodimer NNS cord-290231-4m9lj0uq 189 18 during during IN cord-290231-4m9lj0uq 189 19 exocytosis exocytosis NN cord-290231-4m9lj0uq 189 20 ( ( -LRB- cord-290231-4m9lj0uq 189 21 Wengler Wengler NNP cord-290231-4m9lj0uq 189 22 and and CC cord-290231-4m9lj0uq 189 23 Wengler Wengler NNP cord-290231-4m9lj0uq 189 24 , , , cord-290231-4m9lj0uq 189 25 1989 1989 CD cord-290231-4m9lj0uq 189 26 ) ) -RRB- cord-290231-4m9lj0uq 190 1 we -PRON- PRP cord-290231-4m9lj0uq 190 2 reasoned reason VBD cord-290231-4m9lj0uq 190 3 that that IN cord-290231-4m9lj0uq 190 4 the the DT cord-290231-4m9lj0uq 190 5 function function NN cord-290231-4m9lj0uq 190 6 of of IN cord-290231-4m9lj0uq 190 7 the the DT cord-290231-4m9lj0uq 190 8 prM prm NN cord-290231-4m9lj0uq 190 9 is be VBZ cord-290231-4m9lj0uq 190 10 to to TO cord-290231-4m9lj0uq 190 11 prevent prevent VB cord-290231-4m9lj0uq 190 12 conformational conformational JJ cord-290231-4m9lj0uq 190 13 changes change NNS cord-290231-4m9lj0uq 190 14 in in IN cord-290231-4m9lj0uq 190 15 the the DT cord-290231-4m9lj0uq 190 16 E e NN cord-290231-4m9lj0uq 190 17 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 190 18 at at IN cord-290231-4m9lj0uq 190 19 low low JJ cord-290231-4m9lj0uq 190 20 pH ph NN cord-290231-4m9lj0uq 190 21 , , , cord-290231-4m9lj0uq 190 22 thus thus RB cord-290231-4m9lj0uq 190 23 assuring assure VBG cord-290231-4m9lj0uq 190 24 safe safe JJ cord-290231-4m9lj0uq 190 25 transport transport NN cord-290231-4m9lj0uq 190 26 of of IN cord-290231-4m9lj0uq 190 27 these these DT cord-290231-4m9lj0uq 190 28 proteins protein NNS cord-290231-4m9lj0uq 190 29 through through IN cord-290231-4m9lj0uq 190 30 post post JJ cord-290231-4m9lj0uq 190 31 - - JJ cord-290231-4m9lj0uq 190 32 Golgi golgi JJ cord-290231-4m9lj0uq 190 33 acidic acidic JJ cord-290231-4m9lj0uq 190 34 vesicles vesicle NNS cord-290231-4m9lj0uq 190 35 during during IN cord-290231-4m9lj0uq 190 36 maturation maturation NN cord-290231-4m9lj0uq 190 37 until until IN cord-290231-4m9lj0uq 190 38 the the DT cord-290231-4m9lj0uq 190 39 virions virion NNS cord-290231-4m9lj0uq 190 40 are be VBP cord-290231-4m9lj0uq 190 41 released release VBN cord-290231-4m9lj0uq 190 42 . . . cord-290231-4m9lj0uq 191 1 A a DT cord-290231-4m9lj0uq 191 2 similar similar JJ cord-290231-4m9lj0uq 191 3 role role NN cord-290231-4m9lj0uq 191 4 has have VBZ cord-290231-4m9lj0uq 191 5 also also RB cord-290231-4m9lj0uq 191 6 been be VBN cord-290231-4m9lj0uq 191 7 proposed propose VBN cord-290231-4m9lj0uq 191 8 for for IN cord-290231-4m9lj0uq 191 9 the the DT cord-290231-4m9lj0uq 191 10 PE2 PE2 NNP cord-290231-4m9lj0uq 191 11 of of IN cord-290231-4m9lj0uq 191 12 alphaviruses alphaviruses NNP cord-290231-4m9lj0uq 191 13 , , , cord-290231-4m9lj0uq 191 14 which which WDT cord-290231-4m9lj0uq 191 15 forms form VBZ cord-290231-4m9lj0uq 191 16 a a DT cord-290231-4m9lj0uq 191 17 stable stable JJ cord-290231-4m9lj0uq 191 18 dimer dimer NN cord-290231-4m9lj0uq 191 19 with with IN cord-290231-4m9lj0uq 191 20 the the DT cord-290231-4m9lj0uq 191 21 El El NNP cord-290231-4m9lj0uq 191 22 protein protein NN cord-290231-4m9lj0uq 191 23 ( ( -LRB- cord-290231-4m9lj0uq 191 24 critical critical JJ cord-290231-4m9lj0uq 191 25 for for IN cord-290231-4m9lj0uq 191 26 infection infection NN cord-290231-4m9lj0uq 191 27 and and CC cord-290231-4m9lj0uq 191 28 fusion fusion NN cord-290231-4m9lj0uq 191 29 ) ) -RRB- cord-290231-4m9lj0uq 191 30 in in IN cord-290231-4m9lj0uq 191 31 the the DT cord-290231-4m9lj0uq 191 32 acidic acidic JJ cord-290231-4m9lj0uq 191 33 compartment compartment NN cord-290231-4m9lj0uq 191 34 of of IN cord-290231-4m9lj0uq 191 35 the the DT cord-290231-4m9lj0uq 191 36 exocytic exocytic JJ cord-290231-4m9lj0uq 191 37 route route NN cord-290231-4m9lj0uq 191 38 ( ( -LRB- cord-290231-4m9lj0uq 191 39 Lobigs Lobigs NNP cord-290231-4m9lj0uq 191 40 and and CC cord-290231-4m9lj0uq 191 41 Garoff Garoff NNP cord-290231-4m9lj0uq 191 42 , , , cord-290231-4m9lj0uq 191 43 1990 1990 CD cord-290231-4m9lj0uq 191 44 ; ; : cord-290231-4m9lj0uq 191 45 Presley Presley NNP cord-290231-4m9lj0uq 191 46 et et NNP cord-290231-4m9lj0uq 191 47 a/. a/. NN cord-290231-4m9lj0uq 191 48 , , , cord-290231-4m9lj0uq 191 49 1991 1991 CD cord-290231-4m9lj0uq 191 50 ; ; : cord-290231-4m9lj0uq 191 51 Wahlberg Wahlberg NNP cord-290231-4m9lj0uq 191 52 et et NNP cord-290231-4m9lj0uq 191 53 al al NNP cord-290231-4m9lj0uq 191 54 . . NNP cord-290231-4m9lj0uq 191 55 , , , cord-290231-4m9lj0uq 191 56 1989 1989 CD cord-290231-4m9lj0uq 191 57 ) ) -RRB- cord-290231-4m9lj0uq 191 58 . . . cord-290231-4m9lj0uq 192 1 Different different JJ cord-290231-4m9lj0uq 192 2 approaches approach NNS cord-290231-4m9lj0uq 192 3 might may MD cord-290231-4m9lj0uq 192 4 be be VB cord-290231-4m9lj0uq 192 5 utilized utilize VBN cord-290231-4m9lj0uq 192 6 by by IN cord-290231-4m9lj0uq 192 7 other other JJ cord-290231-4m9lj0uq 192 8 viruses virus NNS cord-290231-4m9lj0uq 192 9 to to TO cord-290231-4m9lj0uq 192 10 secure secure VB cord-290231-4m9lj0uq 192 11 the the DT cord-290231-4m9lj0uq 192 12 authentic authentic JJ cord-290231-4m9lj0uq 192 13 expression expression NN cord-290231-4m9lj0uq 192 14 of of IN cord-290231-4m9lj0uq 192 15 the the DT cord-290231-4m9lj0uq 192 16 envelope envelope NN cord-290231-4m9lj0uq 192 17 proteins protein NNS cord-290231-4m9lj0uq 192 18 on on IN cord-290231-4m9lj0uq 192 19 the the DT cord-290231-4m9lj0uq 192 20 infected infected JJ cord-290231-4m9lj0uq 192 21 cells cell NNS cord-290231-4m9lj0uq 192 22 . . . cord-290231-4m9lj0uq 193 1 It -PRON- PRP cord-290231-4m9lj0uq 193 2 has have VBZ cord-290231-4m9lj0uq 193 3 been be VBN cord-290231-4m9lj0uq 193 4 shown show VBN cord-290231-4m9lj0uq 193 5 that that IN cord-290231-4m9lj0uq 193 6 the the DT cord-290231-4m9lj0uq 193 7 M2 M2 NNP cord-290231-4m9lj0uq 193 8 protein protein NN cord-290231-4m9lj0uq 193 9 of of IN cord-290231-4m9lj0uq 193 10 influ influ NNP cord-290231-4m9lj0uq 193 11 - - HYPH cord-290231-4m9lj0uq 193 12 enza enza NNP cord-290231-4m9lj0uq 193 13 virus virus NN cord-290231-4m9lj0uq 193 14 raises raise VBZ cord-290231-4m9lj0uq 193 15 the the DT cord-290231-4m9lj0uq 193 16 pH ph NN cord-290231-4m9lj0uq 193 17 of of IN cord-290231-4m9lj0uq 193 18 the the DT cord-290231-4m9lj0uq 193 19 post post JJ cord-290231-4m9lj0uq 193 20 - - JJ cord-290231-4m9lj0uq 193 21 Golgi golgi JJ cord-290231-4m9lj0uq 193 22 vesicles vesicle NNS cord-290231-4m9lj0uq 193 23 by by IN cord-290231-4m9lj0uq 193 24 about about RB cord-290231-4m9lj0uq 193 25 0.8 0.8 CD cord-290231-4m9lj0uq 193 26 unit unit NN cord-290231-4m9lj0uq 193 27 , , , cord-290231-4m9lj0uq 193 28 thus thus RB cord-290231-4m9lj0uq 193 29 preventing prevent VBG cord-290231-4m9lj0uq 193 30 the the DT cord-290231-4m9lj0uq 193 31 expression expression NN cord-290231-4m9lj0uq 193 32 of of IN cord-290231-4m9lj0uq 193 33 the the DT cord-290231-4m9lj0uq 193 34 low low JJ cord-290231-4m9lj0uq 193 35 pH pH NNP cord-290231-4m9lj0uq 193 36 conformation conformation NN cord-290231-4m9lj0uq 193 37 of of IN cord-290231-4m9lj0uq 193 38 the the DT cord-290231-4m9lj0uq 193 39 HA HA NNP cord-290231-4m9lj0uq 193 40 molecules molecule NNS cord-290231-4m9lj0uq 193 41 on on IN cord-290231-4m9lj0uq 193 42 the the DT cord-290231-4m9lj0uq 193 43 plasma plasma NN cord-290231-4m9lj0uq 193 44 membranes membrane NNS cord-290231-4m9lj0uq 193 45 of of IN cord-290231-4m9lj0uq 193 46 the the DT cord-290231-4m9lj0uq 193 47 infected infected JJ cord-290231-4m9lj0uq 193 48 cells cell NNS cord-290231-4m9lj0uq 193 49 ( ( -LRB- cord-290231-4m9lj0uq 193 50 Ciampor Ciampor NNP cord-290231-4m9lj0uq 193 51 et et NNP cord-290231-4m9lj0uq 193 52 a/. a/. NN cord-290231-4m9lj0uq 193 53 , , , cord-290231-4m9lj0uq 193 54 1992 1992 CD cord-290231-4m9lj0uq 193 55 ) ) -RRB- cord-290231-4m9lj0uq 193 56 . . . cord-290231-4m9lj0uq 194 1 The the DT cord-290231-4m9lj0uq 194 2 high high JJ cord-290231-4m9lj0uq 194 3 resistance resistance NN cord-290231-4m9lj0uq 194 4 of of IN cord-290231-4m9lj0uq 194 5 the the DT cord-290231-4m9lj0uq 194 6 prM prm NN cord-290231-4m9lj0uq 194 7 - - HYPH cord-290231-4m9lj0uq 194 8 containing contain VBG cord-290231-4m9lj0uq 194 9 viruses virus NNS cord-290231-4m9lj0uq 194 10 to to IN cord-290231-4m9lj0uq 194 11 low low JJ cord-290231-4m9lj0uq 194 12 pH ph NN cord-290231-4m9lj0uq 194 13 might may MD cord-290231-4m9lj0uq 194 14 indicate indicate VB cord-290231-4m9lj0uq 194 15 that that IN cord-290231-4m9lj0uq 194 16 the the DT cord-290231-4m9lj0uq 194 17 prM prM NNP cord-290231-4m9lj0uq 194 18 could could MD cord-290231-4m9lj0uq 194 19 shield shield VB cord-290231-4m9lj0uq 194 20 some some DT cord-290231-4m9lj0uq 194 21 segments segment NNS cord-290231-4m9lj0uq 194 22 of of IN cord-290231-4m9lj0uq 194 23 the the DT cord-290231-4m9lj0uq 194 24 E e NN cord-290231-4m9lj0uq 194 25 protein protein NN cord-290231-4m9lj0uq 194 26 which which WDT cord-290231-4m9lj0uq 194 27 are be VBP cord-290231-4m9lj0uq 194 28 otherwise otherwise RB cord-290231-4m9lj0uq 194 29 protonated protonate VBN cord-290231-4m9lj0uq 194 30 in in IN cord-290231-4m9lj0uq 194 31 a a DT cord-290231-4m9lj0uq 194 32 low low JJ cord-290231-4m9lj0uq 194 33 pH pH NNP cord-290231-4m9lj0uq 194 34 environment environment NN cord-290231-4m9lj0uq 194 35 , , , cord-290231-4m9lj0uq 194 36 leading lead VBG cord-290231-4m9lj0uq 194 37 to to IN cord-290231-4m9lj0uq 194 38 the the DT cord-290231-4m9lj0uq 194 39 exposure exposure NN cord-290231-4m9lj0uq 194 40 of of IN cord-290231-4m9lj0uq 194 41 the the DT cord-290231-4m9lj0uq 194 42 fusion fusion NN cord-290231-4m9lj0uq 194 43 peptide peptide NN cord-290231-4m9lj0uq 194 44 and and CC cord-290231-4m9lj0uq 194 45 consequent consequent JJ cord-290231-4m9lj0uq 194 46 loss loss NN cord-290231-4m9lj0uq 194 47 of of IN cord-290231-4m9lj0uq 194 48 infectivity infectivity NN cord-290231-4m9lj0uq 194 49 . . . cord-290231-4m9lj0uq 195 1 Concomitant concomitant JJ cord-290231-4m9lj0uq 195 2 with with IN cord-290231-4m9lj0uq 195 3 the the DT cord-290231-4m9lj0uq 195 4 low low JJ cord-290231-4m9lj0uq 195 5 pH ph NN cord-290231-4m9lj0uq 195 6 sensitivity sensitivity NN cord-290231-4m9lj0uq 195 7 of of IN cord-290231-4m9lj0uq 195 8 the the DT cord-290231-4m9lj0uq 195 9 M M NNP cord-290231-4m9lj0uq 195 10 - - HYPH cord-290231-4m9lj0uq 195 11 containing contain VBG cord-290231-4m9lj0uq 195 12 viruses virus NNS cord-290231-4m9lj0uq 195 13 was be VBD cord-290231-4m9lj0uq 195 14 a a DT cord-290231-4m9lj0uq 195 15 loss loss NN cord-290231-4m9lj0uq 195 16 of of IN cord-290231-4m9lj0uq 195 17 epitope epitope NN cord-290231-4m9lj0uq 195 18 reactivity reactivity NN cord-290231-4m9lj0uq 195 19 in in IN cord-290231-4m9lj0uq 195 20 ELISA ELISA NNP cord-290231-4m9lj0uq 195 21 following follow VBG cord-290231-4m9lj0uq 195 22 incubation incubation NN cord-290231-4m9lj0uq 195 23 at at IN cord-290231-4m9lj0uq 195 24 pH pH NNP cord-290231-4m9lj0uq 195 25 6.0 6.0 CD cord-290231-4m9lj0uq 195 26 or or CC cord-290231-4m9lj0uq 195 27 below below RB cord-290231-4m9lj0uq 195 28 . . . cord-290231-4m9lj0uq 196 1 Because because IN cord-290231-4m9lj0uq 196 2 prM prm NN cord-290231-4m9lj0uq 196 3 - - HYPH cord-290231-4m9lj0uq 196 4 containing contain VBG cord-290231-4m9lj0uq 196 5 viruses virus NNS cord-290231-4m9lj0uq 196 6 are be VBP cord-290231-4m9lj0uq 196 7 still still RB cord-290231-4m9lj0uq 196 8 infectious infectious JJ cord-290231-4m9lj0uq 196 9 , , , cord-290231-4m9lj0uq 196 10 apparently apparently RB cord-290231-4m9lj0uq 196 11 the the DT cord-290231-4m9lj0uq 196 12 prM prm JJ cord-290231-4m9lj0uq 196 13 protein protein NN cord-290231-4m9lj0uq 196 14 can can MD cord-290231-4m9lj0uq 196 15 be be VB cord-290231-4m9lj0uq 196 16 cleaved cleave VBN cord-290231-4m9lj0uq 196 17 after after IN cord-290231-4m9lj0uq 196 18 the the DT cord-290231-4m9lj0uq 196 19 virus virus NN cord-290231-4m9lj0uq 196 20 has have VBZ cord-290231-4m9lj0uq 196 21 undergone undergo VBN cord-290231-4m9lj0uq 196 22 endocytosis endocytosis NN cord-290231-4m9lj0uq 196 23 . . . cord-290231-4m9lj0uq 197 1 Using use VBG cord-290231-4m9lj0uq 197 2 chymotrypsin chymotrypsin NNP cord-290231-4m9lj0uq 197 3 , , , cord-290231-4m9lj0uq 197 4 trypsin trypsin NN cord-290231-4m9lj0uq 197 5 , , , cord-290231-4m9lj0uq 197 6 and and CC cord-290231-4m9lj0uq 197 7 thermolysin thermolysin NNP cord-290231-4m9lj0uq 197 8 it -PRON- PRP cord-290231-4m9lj0uq 197 9 was be VBD cord-290231-4m9lj0uq 197 10 demonstrated demonstrate VBN cord-290231-4m9lj0uq 197 11 that that IN cord-290231-4m9lj0uq 197 12 the the DT cord-290231-4m9lj0uq 197 13 prM prm JJ cord-290231-4m9lj0uq 197 14 protein protein NN cord-290231-4m9lj0uq 197 15 of of IN cord-290231-4m9lj0uq 197 16 the the DT cord-290231-4m9lj0uq 197 17 WN WN NNP cord-290231-4m9lj0uq 197 18 virus virus NN cord-290231-4m9lj0uq 197 19 is be VBZ cord-290231-4m9lj0uq 197 20 highly highly RB cord-290231-4m9lj0uq 197 21 sensitive sensitive JJ cord-290231-4m9lj0uq 197 22 to to IN cord-290231-4m9lj0uq 197 23 proteases protease NNS cord-290231-4m9lj0uq 197 24 . . . cord-290231-4m9lj0uq 198 1 In in IN cord-290231-4m9lj0uq 198 2 our -PRON- PRP$ cord-290231-4m9lj0uq 198 3 laboratory laboratory NN cord-290231-4m9lj0uq 198 4 we -PRON- PRP cord-290231-4m9lj0uq 198 5 also also RB cord-290231-4m9lj0uq 198 6 observed observe VBD cord-290231-4m9lj0uq 198 7 that that IN cord-290231-4m9lj0uq 198 8 by by IN cord-290231-4m9lj0uq 198 9 using use VBG cord-290231-4m9lj0uq 198 10 a a DT cord-290231-4m9lj0uq 198 11 low low JJ cord-290231-4m9lj0uq 198 12 concentration concentration NN cord-290231-4m9lj0uq 198 13 of of IN cord-290231-4m9lj0uq 198 14 trypsin trypsin NN cord-290231-4m9lj0uq 198 15 , , , cord-290231-4m9lj0uq 198 16 the the DT cord-290231-4m9lj0uq 198 17 prM prm JJ cord-290231-4m9lj0uq 198 18 protein protein NN cord-290231-4m9lj0uq 198 19 of of IN cord-290231-4m9lj0uq 198 20 the the DT cord-290231-4m9lj0uq 198 21 MVE MVE NNP cord-290231-4m9lj0uq 198 22 virus virus NN cord-290231-4m9lj0uq 198 23 is be VBZ cord-290231-4m9lj0uq 198 24 cleaved cleave VBN cord-290231-4m9lj0uq 198 25 prior prior RB cord-290231-4m9lj0uq 198 26 to to IN cord-290231-4m9lj0uq 198 27 the the DT cord-290231-4m9lj0uq 198 28 E e NN cord-290231-4m9lj0uq 198 29 protein protein NN cord-290231-4m9lj0uq 198 30 ( ( -LRB- cord-290231-4m9lj0uq 198 31 data datum NNS cord-290231-4m9lj0uq 198 32 not not RB cord-290231-4m9lj0uq 198 33 shown show VBN cord-290231-4m9lj0uq 198 34 ) ) -RRB- cord-290231-4m9lj0uq 198 35 . . . cord-290231-4m9lj0uq 199 1 Our -PRON- PRP$ cord-290231-4m9lj0uq 199 2 results result NNS cord-290231-4m9lj0uq 199 3 predict predict VBP cord-290231-4m9lj0uq 199 4 that that IN cord-290231-4m9lj0uq 199 5 the the DT cord-290231-4m9lj0uq 199 6 expression expression NN cord-290231-4m9lj0uq 199 7 of of IN cord-290231-4m9lj0uq 199 8 authentic authentic JJ cord-290231-4m9lj0uq 199 9 E e NN cord-290231-4m9lj0uq 199 10 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 199 11 requires require VBZ cord-290231-4m9lj0uq 199 12 the the DT cord-290231-4m9lj0uq 199 13 coexpression coexpression NN cord-290231-4m9lj0uq 199 14 of of IN cord-290231-4m9lj0uq 199 15 prM. prm. NN cord-290231-4m9lj0uq 200 1 This this DT cord-290231-4m9lj0uq 200 2 would would MD cord-290231-4m9lj0uq 200 3 explain explain VB cord-290231-4m9lj0uq 200 4 why why WRB cord-290231-4m9lj0uq 200 5 flavivirus flavivirus JJ cord-290231-4m9lj0uq 200 6 vaccine vaccine NN cord-290231-4m9lj0uq 200 7 candidates candidate NNS cord-290231-4m9lj0uq 200 8 produced produce VBN cord-290231-4m9lj0uq 200 9 from from IN cord-290231-4m9lj0uq 200 10 the the DT cord-290231-4m9lj0uq 200 11 expression expression NN cord-290231-4m9lj0uq 200 12 of of IN cord-290231-4m9lj0uq 200 13 only only JJ cord-290231-4m9lj0uq 200 14 E e NN cord-290231-4m9lj0uq 200 15 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 200 16 were be VBD cord-290231-4m9lj0uq 200 17 partially partially RB cord-290231-4m9lj0uq 200 18 protective protective JJ cord-290231-4m9lj0uq 200 19 against against IN cord-290231-4m9lj0uq 200 20 homotypic homotypic JJ cord-290231-4m9lj0uq 200 21 challenge challenge NN cord-290231-4m9lj0uq 200 22 , , , cord-290231-4m9lj0uq 200 23 whereas whereas IN cord-290231-4m9lj0uq 200 24 those those DT cord-290231-4m9lj0uq 200 25 prepared prepare VBN cord-290231-4m9lj0uq 200 26 from from IN cord-290231-4m9lj0uq 200 27 the the DT cord-290231-4m9lj0uq 200 28 expression expression NN cord-290231-4m9lj0uq 200 29 of of IN cord-290231-4m9lj0uq 200 30 both both DT cord-290231-4m9lj0uq 200 31 prM prm JJ cord-290231-4m9lj0uq 200 32 and and CC cord-290231-4m9lj0uq 200 33 E e NN cord-290231-4m9lj0uq 200 34 glycoproteins glycoprotein NNS cord-290231-4m9lj0uq 200 35 gave give VBD cord-290231-4m9lj0uq 200 36 a a DT cord-290231-4m9lj0uq 200 37 significant significant JJ cord-290231-4m9lj0uq 200 38 level level NN cord-290231-4m9lj0uq 200 39 of of IN cord-290231-4m9lj0uq 200 40 protection protection NN cord-290231-4m9lj0uq 200 41 ( ( -LRB- cord-290231-4m9lj0uq 200 42 Bray Bray NNP cord-290231-4m9lj0uq 200 43 and and CC cord-290231-4m9lj0uq 200 44 Lai Lai NNP cord-290231-4m9lj0uq 200 45 , , , cord-290231-4m9lj0uq 200 46 1991 1991 CD cord-290231-4m9lj0uq 200 47 ) ) -RRB- cord-290231-4m9lj0uq 200 48 . . . cord-290231-4m9lj0uq 201 1 Similar similar JJ cord-290231-4m9lj0uq 201 2 results result NNS cord-290231-4m9lj0uq 201 3 were be VBD cord-290231-4m9lj0uq 201 4 observed observe VBN cord-290231-4m9lj0uq 201 5 with with IN cord-290231-4m9lj0uq 201 6 Japanese japanese JJ cord-290231-4m9lj0uq 201 7 encephalitis encephalitis JJ cord-290231-4m9lj0uq 201 8 virus virus NN cord-290231-4m9lj0uq 201 9 , , , cord-290231-4m9lj0uq 201 10 where where WRB cord-290231-4m9lj0uq 201 11 high high JJ cord-290231-4m9lj0uq 201 12 neutralizing neutralizing NN cord-290231-4m9lj0uq 201 13 and and CC cord-290231-4m9lj0uq 201 14 hemagglutination hemagglutination NN cord-290231-4m9lj0uq 201 15 - - HYPH cord-290231-4m9lj0uq 201 16 inhibiting inhibit VBG cord-290231-4m9lj0uq 201 17 antibodies antibody NNS cord-290231-4m9lj0uq 201 18 which which WDT cord-290231-4m9lj0uq 201 19 correlated correlate VBD cord-290231-4m9lj0uq 201 20 with with IN cord-290231-4m9lj0uq 201 21 a a DT cord-290231-4m9lj0uq 201 22 high high JJ cord-290231-4m9lj0uq 201 23 level level NN cord-290231-4m9lj0uq 201 24 of of IN cord-290231-4m9lj0uq 201 25 protection protection NN cord-290231-4m9lj0uq 201 26 were be VBD cord-290231-4m9lj0uq 201 27 found find VBN cord-290231-4m9lj0uq 201 28 in in IN cord-290231-4m9lj0uq 201 29 mice mouse NNS cord-290231-4m9lj0uq 201 30 inoculated inoculate VBN cord-290231-4m9lj0uq 201 31 with with IN cord-290231-4m9lj0uq 201 32 recombinant recombinant JJ cord-290231-4m9lj0uq 201 33 vaccine vaccine NN cord-290231-4m9lj0uq 201 34 viruses virus NNS cord-290231-4m9lj0uq 201 35 expressing express VBG cord-290231-4m9lj0uq 201 36 both both CC cord-290231-4m9lj0uq 201 37 prM prm JJ cord-290231-4m9lj0uq 201 38 and and CC cord-290231-4m9lj0uq 201 39 E e NN cord-290231-4m9lj0uq 201 40 but but CC cord-290231-4m9lj0uq 201 41 not not RB cord-290231-4m9lj0uq 201 42 E e FW cord-290231-4m9lj0uq 201 43 pro pro JJ cord-290231-4m9lj0uq 201 44 - - JJ cord-290231-4m9lj0uq 201 45 tein tein NNP cord-290231-4m9lj0uq 201 46 alone alone RB cord-290231-4m9lj0uq 202 1 ( ( -LRB- cord-290231-4m9lj0uq 202 2 Konishi Konishi NNP cord-290231-4m9lj0uq 202 3 et et FW cord-290231-4m9lj0uq 202 4 al al NNP cord-290231-4m9lj0uq 202 5 . . NNP cord-290231-4m9lj0uq 202 6 , , , cord-290231-4m9lj0uq 202 7 1991 1991 CD cord-290231-4m9lj0uq 203 1 ; ; : cord-290231-4m9lj0uq 203 2 Yasuda Yasuda NNP cord-290231-4m9lj0uq 203 3 et et NNP cord-290231-4m9lj0uq 203 4 a/. a/. NNP cord-290231-4m9lj0uq 203 5 , , , cord-290231-4m9lj0uq 203 6 1990 1990 CD cord-290231-4m9lj0uq 203 7 ) ) -RRB- cord-290231-4m9lj0uq 203 8 . . . cord-290231-4m9lj0uq 204 1 We -PRON- PRP cord-290231-4m9lj0uq 204 2 are be VBP cord-290231-4m9lj0uq 204 3 currently currently RB cord-290231-4m9lj0uq 204 4 performing perform VBG cord-290231-4m9lj0uq 204 5 experiments experiment NNS cord-290231-4m9lj0uq 204 6 to to TO cord-290231-4m9lj0uq 204 7 define define VB cord-290231-4m9lj0uq 204 8 further further RB cord-290231-4m9lj0uq 204 9 the the DT cord-290231-4m9lj0uq 204 10 role role NN cord-290231-4m9lj0uq 204 11 of of IN cord-290231-4m9lj0uq 204 12 prM prm NN cord-290231-4m9lj0uq 204 13 in in IN cord-290231-4m9lj0uq 204 14 virus virus NN cord-290231-4m9lj0uq 204 15 infectivity infectivity NN cord-290231-4m9lj0uq 204 16 and and CC cord-290231-4m9lj0uq 204 17 the the DT cord-290231-4m9lj0uq 204 18 events event NNS cord-290231-4m9lj0uq 204 19 occurring occur VBG cord-290231-4m9lj0uq 204 20 after after IN cord-290231-4m9lj0uq 204 21 prM prm NN cord-290231-4m9lj0uq 204 22 - - HYPH cord-290231-4m9lj0uq 204 23 containing contain VBG cord-290231-4m9lj0uq 204 24 viruses virus NNS cord-290231-4m9lj0uq 204 25 enter enter VBP cord-290231-4m9lj0uq 204 26 their -PRON- PRP$ cord-290231-4m9lj0uq 204 27 target target NN cord-290231-4m9lj0uq 204 28 cells cell NNS cord-290231-4m9lj0uq 204 29 . . . cord-290231-4m9lj0uq 205 1 We -PRON- PRP cord-290231-4m9lj0uq 205 2 are be VBP cord-290231-4m9lj0uq 205 3 also also RB cord-290231-4m9lj0uq 205 4 attempting attempt VBG cord-290231-4m9lj0uq 205 5 to to TO cord-290231-4m9lj0uq 205 6 determine determine VB cord-290231-4m9lj0uq 205 7 if if IN cord-290231-4m9lj0uq 205 8 a a DT cord-290231-4m9lj0uq 205 9 similar similar JJ cord-290231-4m9lj0uq 205 10 area area NN cord-290231-4m9lj0uq 205 11 on on IN cord-290231-4m9lj0uq 205 12 the the DT cord-290231-4m9lj0uq 205 13 E e NN cord-290231-4m9lj0uq 205 14 protein protein NN cord-290231-4m9lj0uq 205 15 of of IN cord-290231-4m9lj0uq 205 16 other other JJ cord-290231-4m9lj0uq 205 17 flaviviruses flavivirus NNS cord-290231-4m9lj0uq 205 18 is be VBZ cord-290231-4m9lj0uq 205 19 involved involve VBN cord-290231-4m9lj0uq 205 20 in in IN cord-290231-4m9lj0uq 205 21 the the DT cord-290231-4m9lj0uq 205 22 prM prm JJ cord-290231-4m9lj0uq 205 23 interaction interaction NN cord-290231-4m9lj0uq 205 24 . . . cord-290231-4m9lj0uq 206 1 Dengue dengue NN cord-290231-4m9lj0uq 206 2 virus virus NN cord-290231-4m9lj0uq 206 3 premembrane premembrane NN cord-290231-4m9lj0uq 206 4 and and CC cord-290231-4m9lj0uq 206 5 membrane membrane NN cord-290231-4m9lj0uq 206 6 proteins protein NNS cord-290231-4m9lj0uq 206 7 elicit elicit VBP cord-290231-4m9lj0uq 206 8 a a DT cord-290231-4m9lj0uq 206 9 protective protective JJ cord-290231-4m9lj0uq 206 10 immune immune JJ cord-290231-4m9lj0uq 206 11 response response NN cord-290231-4m9lj0uq 206 12 . . . cord-290231-4m9lj0uq 207 1 L L NNP cord-290231-4m9lj0uq 207 2 / / SYM cord-290231-4m9lj0uq 207 3 iro iro NNP cord-290231-4m9lj0uq 207 4 / / SYM cord-290231-4m9lj0uq 207 5 ogy ogy NN cord-290231-4m9lj0uq 207 6 185 185 CD cord-290231-4m9lj0uq 207 7 Regulation Regulation NNP cord-290231-4m9lj0uq 207 8 of of IN cord-290231-4m9lj0uq 207 9 pH pH NNP cord-290231-4m9lj0uq 207 10 by by IN cord-290231-4m9lj0uq 207 11 the the DT cord-290231-4m9lj0uq 207 12 M2 M2 NNP cord-290231-4m9lj0uq 207 13 protein protein NN cord-290231-4m9lj0uq 207 14 of of IN cord-290231-4m9lj0uq 207 15 influenza influenza NN cord-290231-4m9lj0uq 207 16 A a NN cord-290231-4m9lj0uq 207 17 viruses virus NNS cord-290231-4m9lj0uq 207 18 Partial partial JJ cord-290231-4m9lj0uq 207 19 nucleotide nucleotide JJ cord-290231-4m9lj0uq 207 20 sequence sequence NN cord-290231-4m9lj0uq 207 21 of of IN cord-290231-4m9lj0uq 207 22 the the DT cord-290231-4m9lj0uq 207 23 Murray Murray NNP cord-290231-4m9lj0uq 207 24 Valley Valley NNP cord-290231-4m9lj0uq 207 25 encephalitis encephalitis NN cord-290231-4m9lj0uq 207 26 virus virus NN cord-290231-4m9lj0uq 207 27 genome genome NN cord-290231-4m9lj0uq 207 28 : : : cord-290231-4m9lj0uq 207 29 Comparison comparison NN cord-290231-4m9lj0uq 207 30 of of IN cord-290231-4m9lj0uq 207 31 the the DT cord-290231-4m9lj0uq 207 32 encoded encode VBN cord-290231-4m9lj0uq 207 33 polypeptides polypeptide NNS cord-290231-4m9lj0uq 207 34 with with IN cord-290231-4m9lj0uq 207 35 Yellow Yellow NNP cord-290231-4m9lj0uq 207 36 Fever Fever NNP cord-290231-4m9lj0uq 207 37 virus virus NN cord-290231-4m9lj0uq 207 38 structural structural JJ cord-290231-4m9lj0uq 207 39 and and CC cord-290231-4m9lj0uq 207 40 non non JJ cord-290231-4m9lj0uq 207 41 - - JJ cord-290231-4m9lj0uq 207 42 structural structural JJ cord-290231-4m9lj0uq 207 43 proteins protein NNS cord-290231-4m9lj0uq 207 44 Membrane membrane NN cord-290231-4m9lj0uq 207 45 fusion fusion NN cord-290231-4m9lj0uq 207 46 activity activity NN cord-290231-4m9lj0uq 207 47 of of IN cord-290231-4m9lj0uq 207 48 the the DT cord-290231-4m9lj0uq 207 49 influenza influenza NN cord-290231-4m9lj0uq 207 50 virus virus NN cord-290231-4m9lj0uq 207 51 hemagglutinin hemagglutinin NNP cord-290231-4m9lj0uq 207 52 : : : cord-290231-4m9lj0uq 207 53 The the DT cord-290231-4m9lj0uq 207 54 low low JJ cord-290231-4m9lj0uq 207 55 pH ph NN cord-290231-4m9lj0uq 207 56 - - HYPH cord-290231-4m9lj0uq 207 57 induced induce VBN cord-290231-4m9lj0uq 207 58 conformational conformational JJ cord-290231-4m9lj0uq 207 59 change change NN cord-290231-4m9lj0uq 207 60 Prohormone Prohormone NNP cord-290231-4m9lj0uq 207 61 processing processing NN cord-290231-4m9lj0uq 207 62 and and CC cord-290231-4m9lj0uq 207 63 the the DT cord-290231-4m9lj0uq 207 64 secretory secretory JJ cord-290231-4m9lj0uq 207 65 pathway pathway NN cord-290231-4m9lj0uq 207 66 Epitope Epitope NNP cord-290231-4m9lj0uq 207 67 model model NN cord-290231-4m9lj0uq 207 68 of of IN cord-290231-4m9lj0uq 207 69 tick tick NN cord-290231-4m9lj0uq 207 70 - - HYPH cord-290231-4m9lj0uq 207 71 borne bear VBN cord-290231-4m9lj0uq 207 72 encephalitis encephalitis JJ cord-290231-4m9lj0uq 207 73 virus virus NN cord-290231-4m9lj0uq 207 74 envelope envelope NN cord-290231-4m9lj0uq 207 75 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 207 76 E e NN cord-290231-4m9lj0uq 207 77 : : : cord-290231-4m9lj0uq 207 78 Analysis analysis NN cord-290231-4m9lj0uq 207 79 of of IN cord-290231-4m9lj0uq 207 80 structural structural JJ cord-290231-4m9lj0uq 207 81 properties property NNS cord-290231-4m9lj0uq 207 82 , , , cord-290231-4m9lj0uq 207 83 role role NN cord-290231-4m9lj0uq 207 84 of of IN cord-290231-4m9lj0uq 207 85 carbohydrate carbohydrate NN cord-290231-4m9lj0uq 207 86 side side NN cord-290231-4m9lj0uq 207 87 chain chain NN cord-290231-4m9lj0uq 207 88 , , , cord-290231-4m9lj0uq 207 89 and and CC cord-290231-4m9lj0uq 207 90 conformational conformational JJ cord-290231-4m9lj0uq 207 91 changes change NNS cord-290231-4m9lj0uq 207 92 occurring occur VBG cord-290231-4m9lj0uq 207 93 at at IN cord-290231-4m9lj0uq 207 94 acidic acidic JJ cord-290231-4m9lj0uq 207 95 pH pH NNP cord-290231-4m9lj0uq 207 96 Fusion fusion NN cord-290231-4m9lj0uq 207 97 activity activity NN cord-290231-4m9lj0uq 207 98 of of IN cord-290231-4m9lj0uq 207 99 flaviviruses flavivirus NNS cord-290231-4m9lj0uq 207 100 : : : cord-290231-4m9lj0uq 207 101 Comparison comparison NN cord-290231-4m9lj0uq 207 102 of of IN cord-290231-4m9lj0uq 207 103 mature mature JJ cord-290231-4m9lj0uq 207 104 and and CC cord-290231-4m9lj0uq 207 105 immature immature JJ cord-290231-4m9lj0uq 207 106 ( ( -LRB- cord-290231-4m9lj0uq 207 107 prM prm JJ cord-290231-4m9lj0uq 207 108 - - HYPH cord-290231-4m9lj0uq 207 109 containing contain VBG cord-290231-4m9lj0uq 207 110 ) ) -RRB- cord-290231-4m9lj0uq 207 111 tick tick VB cord-290231-4m9lj0uq 207 112 - - HYPH cord-290231-4m9lj0uq 207 113 borne bear VBN cord-290231-4m9lj0uq 207 114 encephalitis encephalitis JJ cord-290231-4m9lj0uq 207 115 virions virion NNS cord-290231-4m9lj0uq 207 116 Antigenic antigenic JJ cord-290231-4m9lj0uq 207 117 structure structure NN cord-290231-4m9lj0uq 207 118 of of IN cord-290231-4m9lj0uq 207 119 the the DT cord-290231-4m9lj0uq 207 120 Murray Murray NNP cord-290231-4m9lj0uq 207 121 Valley Valley NNP cord-290231-4m9lj0uq 207 122 encephalitis encephalitis NN cord-290231-4m9lj0uq 207 123 virus virus NN cord-290231-4m9lj0uq 207 124 E e NN cord-290231-4m9lj0uq 207 125 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 207 126 Flaviviruses flavivirus NNS cord-290231-4m9lj0uq 207 127 : : : cord-290231-4m9lj0uq 207 128 The the DT cord-290231-4m9lj0uq 207 129 basis basis NN cord-290231-4m9lj0uq 207 130 for for IN cord-290231-4m9lj0uq 207 131 serodiagnosis serodiagnosis NNP cord-290231-4m9lj0uq 207 132 and and CC cord-290231-4m9lj0uq 207 133 vaccines vaccine NNS cord-290231-4m9lj0uq 207 134 Synthetic synthetic JJ cord-290231-4m9lj0uq 207 135 peptides peptide NNS cord-290231-4m9lj0uq 207 136 of of IN cord-290231-4m9lj0uq 207 137 the the DT cord-290231-4m9lj0uq 207 138 E2 E2 NNP cord-290231-4m9lj0uq 207 139 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 207 140 of of IN cord-290231-4m9lj0uq 207 141 Venezuelan venezuelan JJ cord-290231-4m9lj0uq 207 142 equine equine JJ cord-290231-4m9lj0uq 207 143 encephalomyelitis encephalomyelitis NN cord-290231-4m9lj0uq 207 144 virus virus NN cord-290231-4m9lj0uq 207 145 . . . cord-290231-4m9lj0uq 208 1 II II NNP cord-290231-4m9lj0uq 208 2 . . . cord-290231-4m9lj0uq 209 1 Antibody antibody NN cord-290231-4m9lj0uq 209 2 to to IN cord-290231-4m9lj0uq 209 3 the the DT cord-290231-4m9lj0uq 209 4 amino amino NN cord-290231-4m9lj0uq 209 5 terminus terminus NN cord-290231-4m9lj0uq 209 6 protects protect VBZ cord-290231-4m9lj0uq 209 7 animals animal NNS cord-290231-4m9lj0uq 209 8 by by IN cord-290231-4m9lj0uq 209 9 limiting limit VBG cord-290231-4m9lj0uq 209 10 viral viral JJ cord-290231-4m9lj0uq 209 11 replication replication NN cord-290231-4m9lj0uq 209 12 Variants variant NNS cord-290231-4m9lj0uq 209 13 of of IN cord-290231-4m9lj0uq 209 14 Venezuelan venezuelan JJ cord-290231-4m9lj0uq 209 15 equine equine NN cord-290231-4m9lj0uq 209 16 encephalitis encephalitis NN cord-290231-4m9lj0uq 209 17 virus virus NN cord-290231-4m9lj0uq 209 18 that that WDT cord-290231-4m9lj0uq 209 19 resist resist VBP cord-290231-4m9lj0uq 209 20 neutralization neutralization NN cord-290231-4m9lj0uq 209 21 define define VB cord-290231-4m9lj0uq 209 22 a a DT cord-290231-4m9lj0uq 209 23 domain domain NN cord-290231-4m9lj0uq 209 24 of of IN cord-290231-4m9lj0uq 209 25 the the DT cord-290231-4m9lj0uq 209 26 E2 E2 NNP cord-290231-4m9lj0uq 209 27 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 209 28 pH ph NN cord-290231-4m9lj0uq 209 29 - - HYPH cord-290231-4m9lj0uq 209 30 Induced induce VBN cord-290231-4m9lj0uq 209 31 alterations alteration NNS cord-290231-4m9lj0uq 209 32 in in IN cord-290231-4m9lj0uq 209 33 the the DT cord-290231-4m9lj0uq 209 34 fusogenic fusogenic JJ cord-290231-4m9lj0uq 209 35 spike spike NN cord-290231-4m9lj0uq 209 36 protein protein NN cord-290231-4m9lj0uq 209 37 of of IN cord-290231-4m9lj0uq 209 38 Semliki Semliki NNP cord-290231-4m9lj0uq 209 39 Forest Forest NNP cord-290231-4m9lj0uq 209 40 virus virus NN cord-290231-4m9lj0uq 209 41 . . . cord-290231-4m9lj0uq 209 42 /. /. . cord-290231-4m9lj0uq 210 1 Cell cell NN cord-290231-4m9lj0uq 210 2 f?iol f?iol NN cord-290231-4m9lj0uq 210 3 Comparison comparison NN cord-290231-4m9lj0uq 210 4 of of IN cord-290231-4m9lj0uq 210 5 protective protective JJ cord-290231-4m9lj0uq 210 6 immunityelicited immunityelicite VBN cord-290231-4m9lj0uq 210 7 by by IN cord-290231-4m9lj0uq 210 8 recombinant recombinant JJ cord-290231-4m9lj0uq 210 9 vaccinia vaccinia NN cord-290231-4m9lj0uq 210 10 viruses virus NNS cord-290231-4m9lj0uq 210 11 that that WDT cord-290231-4m9lj0uq 210 12 synthesize synthesize VBP cord-290231-4m9lj0uq 210 13 E e NN cord-290231-4m9lj0uq 210 14 or or CC cord-290231-4m9lj0uq 210 15 NSl NSl NNS cord-290231-4m9lj0uq 210 16 of of IN cord-290231-4m9lj0uq 210 17 Japanese japanese JJ cord-290231-4m9lj0uq 210 18 encephalitis encephalitis RB cord-290231-4m9lj0uq 210 19 virus virus NN cord-290231-4m9lj0uq 210 20 Cleavage cleavage NN cord-290231-4m9lj0uq 210 21 of of IN cord-290231-4m9lj0uq 210 22 structural structural JJ cord-290231-4m9lj0uq 210 23 proteins protein NNS cord-290231-4m9lj0uq 210 24 during during IN cord-290231-4m9lj0uq 210 25 the the DT cord-290231-4m9lj0uq 210 26 assembly assembly NN cord-290231-4m9lj0uq 210 27 of of IN cord-290231-4m9lj0uq 210 28 the the DT cord-290231-4m9lj0uq 210 29 head head NN cord-290231-4m9lj0uq 210 30 of of IN cord-290231-4m9lj0uq 210 31 bacteriophage bacteriophage NN cord-290231-4m9lj0uq 210 32 T4 T4 NNP cord-290231-4m9lj0uq 210 33 Fusion Fusion NNP cord-290231-4m9lj0uq 210 34 function function NN cord-290231-4m9lj0uq 210 35 of of IN cord-290231-4m9lj0uq 210 36 the the DT cord-290231-4m9lj0uq 210 37 Semliki Semliki NNP cord-290231-4m9lj0uq 210 38 Forest Forest NNP cord-290231-4m9lj0uq 210 39 virus virus NN cord-290231-4m9lj0uq 210 40 spike spike NN cord-290231-4m9lj0uq 210 41 is be VBZ cord-290231-4m9lj0uq 210 42 activated activate VBN cord-290231-4m9lj0uq 210 43 by by IN cord-290231-4m9lj0uq 210 44 proteolytlc proteolytlc NN cord-290231-4m9lj0uq 210 45 cleavage cleavage NN cord-290231-4m9lj0uq 210 46 of of IN cord-290231-4m9lj0uq 210 47 the the DT cord-290231-4m9lj0uq 210 48 envelope envelope NN cord-290231-4m9lj0uq 210 49 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 210 50 precursor precursor NN cord-290231-4m9lj0uq 210 51 ~62 ~62 NFP cord-290231-4m9lj0uq 210 52 Spike Spike NNP cord-290231-4m9lj0uq 210 53 protein protein NN cord-290231-4m9lj0uq 210 54 ollgomenzation ollgomenzation NN cord-290231-4m9lj0uq 210 55 control control NN cord-290231-4m9lj0uq 210 56 of of IN cord-290231-4m9lj0uq 210 57 Semliki Semliki NNP cord-290231-4m9lj0uq 210 58 Forest Forest NNP cord-290231-4m9lj0uq 210 59 virus virus NN cord-290231-4m9lj0uq 210 60 fusion fusion NN cord-290231-4m9lj0uq 210 61 Antigenic antigenic JJ cord-290231-4m9lj0uq 210 62 structure structure NN cord-290231-4m9lj0uq 210 63 of of IN cord-290231-4m9lj0uq 210 64 the the DT cord-290231-4m9lj0uq 210 65 flavivirus flavivirus NNP cord-290231-4m9lj0uq 210 66 envelope envelope NN cord-290231-4m9lj0uq 210 67 protein protein NN cord-290231-4m9lj0uq 210 68 E E NNP cord-290231-4m9lj0uq 210 69 at at IN cord-290231-4m9lj0uq 210 70 the the DT cord-290231-4m9lj0uq 210 71 molecular molecular JJ cord-290231-4m9lj0uq 210 72 level level NN cord-290231-4m9lj0uq 210 73 , , , cord-290231-4m9lj0uq 210 74 using use VBG cord-290231-4m9lj0uq 210 75 tick tick NN cord-290231-4m9lj0uq 210 76 - - HYPH cord-290231-4m9lj0uq 210 77 borne bear VBN cord-290231-4m9lj0uq 210 78 encephalitis encephalitis JJ cord-290231-4m9lj0uq 210 79 virus virus NN cord-290231-4m9lj0uq 210 80 as as IN cord-290231-4m9lj0uq 210 81 a a DT cord-290231-4m9lj0uq 210 82 model model NN cord-290231-4m9lj0uq 210 83 Japanese japanese JJ cord-290231-4m9lj0uq 210 84 encephalitis encephalitis JJ cord-290231-4m9lj0uq 210 85 virus virus NN cord-290231-4m9lj0uq 210 86 - - HYPH cord-290231-4m9lj0uq 210 87 vaccinia vaccinia NN cord-290231-4m9lj0uq 210 88 recombinants recombinant NNS cord-290231-4m9lj0uq 210 89 produce produce VBP cord-290231-4m9lj0uq 210 90 particulate particulate NN cord-290231-4m9lj0uq 210 91 forms form NNS cord-290231-4m9lj0uq 210 92 of of IN cord-290231-4m9lj0uq 210 93 the the DT cord-290231-4m9lj0uq 210 94 structural structural JJ cord-290231-4m9lj0uq 210 95 membrane membrane NN cord-290231-4m9lj0uq 210 96 proteins protein NNS cord-290231-4m9lj0uq 210 97 and and CC cord-290231-4m9lj0uq 210 98 induce induce VB cord-290231-4m9lj0uq 210 99 high high JJ cord-290231-4m9lj0uq 210 100 levels level NNS cord-290231-4m9lj0uq 210 101 of of IN cord-290231-4m9lj0uq 210 102 protection protection NN cord-290231-4m9lj0uq 210 103 against against IN cord-290231-4m9lj0uq 210 104 lethal lethal JJ cord-290231-4m9lj0uq 210 105 JEV JEV NNP cord-290231-4m9lj0uq 210 106 infection infection NN cord-290231-4m9lj0uq 211 1 Endoproteolytic endoproteolytic JJ cord-290231-4m9lj0uq 211 2 cleavage cleavage NN cord-290231-4m9lj0uq 211 3 of of IN cord-290231-4m9lj0uq 211 4 gpl60 gpl60 CD cord-290231-4m9lj0uq 211 5 is be VBZ cord-290231-4m9lj0uq 211 6 required require VBN cord-290231-4m9lj0uq 211 7 for for IN cord-290231-4m9lj0uq 211 8 the the DT cord-290231-4m9lj0uq 211 9 activation activation NN cord-290231-4m9lj0uq 211 10 of of IN cord-290231-4m9lj0uq 211 11 human human JJ cord-290231-4m9lj0uq 211 12 immunodeficiency immunodeficiency NN cord-290231-4m9lj0uq 211 13 virus virus NN cord-290231-4m9lj0uq 211 14 Fusion Fusion NNP cord-290231-4m9lj0uq 211 15 of of IN cord-290231-4m9lj0uq 211 16 enveloped envelop VBN cord-290231-4m9lj0uq 211 17 viruses virus NNS cord-290231-4m9lj0uq 211 18 with with IN cord-290231-4m9lj0uq 211 19 cells cell NNS cord-290231-4m9lj0uq 211 20 and and CC cord-290231-4m9lj0uq 211 21 liposomes liposome NNS cord-290231-4m9lj0uq 211 22 : : : cord-290231-4m9lj0uq 211 23 Activity activity NN cord-290231-4m9lj0uq 211 24 and and CC cord-290231-4m9lj0uq 211 25 Inactivation Inactivation NNP cord-290231-4m9lj0uq 211 26 . . . cord-290231-4m9lj0uq 212 1 ln ln NNP cord-290231-4m9lj0uq 212 2 Analysis Analysis NNP cord-290231-4m9lj0uq 212 3 of of IN cord-290231-4m9lj0uq 212 4 disulfides disulfides NNP cord-290231-4m9lj0uq 212 5 present present JJ cord-290231-4m9lj0uq 213 1 In in IN cord-290231-4m9lj0uq 213 2 the the DT cord-290231-4m9lj0uq 213 3 membrane membrane NN cord-290231-4m9lj0uq 213 4 proteins protein NNS cord-290231-4m9lj0uq 213 5 of of IN cord-290231-4m9lj0uq 213 6 the the DT cord-290231-4m9lj0uq 213 7 West West NNP cord-290231-4m9lj0uq 213 8 Nile Nile NNP cord-290231-4m9lj0uq 213 9 flavivirus flavivirus JJ cord-290231-4m9lj0uq 213 10 Structural structural JJ cord-290231-4m9lj0uq 213 11 proteins protein NNS cord-290231-4m9lj0uq 213 12 of of IN cord-290231-4m9lj0uq 213 13 La La NNP cord-290231-4m9lj0uq 213 14 Crosse Crosse NNP cord-290231-4m9lj0uq 213 15 virus virus NN cord-290231-4m9lj0uq 213 16 Proteolytic proteolytic JJ cord-290231-4m9lj0uq 213 17 processing processing NN cord-290231-4m9lj0uq 213 18 of of IN cord-290231-4m9lj0uq 213 19 the the DT cord-290231-4m9lj0uq 213 20 Sindbis Sindbis NNP cord-290231-4m9lj0uq 213 21 virus virus NN cord-290231-4m9lj0uq 213 22 membrane membrane NN cord-290231-4m9lj0uq 213 23 protein protein NN cord-290231-4m9lj0uq 213 24 precursor precursor NN cord-290231-4m9lj0uq 213 25 PE2 PE2 NNP cord-290231-4m9lj0uq 213 26 is be VBZ cord-290231-4m9lj0uq 213 27 nonessential nonessential JJ cord-290231-4m9lj0uq 213 28 for for IN cord-290231-4m9lj0uq 213 29 growth growth NN cord-290231-4m9lj0uq 213 30 in in IN cord-290231-4m9lj0uq 213 31 vertebrate vertebrate JJ cord-290231-4m9lj0uq 213 32 cells cell NNS cord-290231-4m9lj0uq 213 33 but but CC cord-290231-4m9lj0uq 213 34 is be VBZ cord-290231-4m9lj0uq 213 35 required require VBN cord-290231-4m9lj0uq 213 36 for for IN cord-290231-4m9lj0uq 213 37 efficient efficient JJ cord-290231-4m9lj0uq 213 38 growth growth NN cord-290231-4m9lj0uq 213 39 in in IN cord-290231-4m9lj0uq 213 40 Invertebrate invertebrate JJ cord-290231-4m9lj0uq 213 41 cells cell NNS cord-290231-4m9lj0uq 214 1 Acidotropic Acidotropic NNP cord-290231-4m9lj0uq 214 2 amines amine NNS cord-290231-4m9lj0uq 214 3 inhibit inhibit VBP cord-290231-4m9lj0uq 214 4 proteolytic proteolytic JJ cord-290231-4m9lj0uq 214 5 processing processing NN cord-290231-4m9lj0uq 214 6 of of IN cord-290231-4m9lj0uq 214 7 flavivirus flavivirus JJ cord-290231-4m9lj0uq 214 8 prM prm JJ cord-290231-4m9lj0uq 214 9 protein protein NN cord-290231-4m9lj0uq 214 10 . . . cord-290231-4m9lj0uq 215 1 Viral Viral NNP cord-290231-4m9lj0uq 215 2 - - HYPH cord-290231-4m9lj0uq 215 3 ogy174 ogy174 NNP cord-290231-4m9lj0uq 215 4 Structure Structure NNP cord-290231-4m9lj0uq 215 5 of of IN cord-290231-4m9lj0uq 215 6 the the DT cord-290231-4m9lj0uq 215 7 flavivirus flavivirus NNP cord-290231-4m9lj0uq 215 8 genome genome NN cord-290231-4m9lj0uq 215 9 Synthetic synthetic JJ cord-290231-4m9lj0uq 215 10 peptides peptide NNS cord-290231-4m9lj0uq 215 11 derived derive VBN cord-290231-4m9lj0uq 215 12 from from IN cord-290231-4m9lj0uq 215 13 the the DT cord-290231-4m9lj0uq 215 14 deduced deduce VBN cord-290231-4m9lj0uq 215 15 amino amino NN cord-290231-4m9lj0uq 215 16 acid acid NN cord-290231-4m9lj0uq 215 17 sequence sequence NN cord-290231-4m9lj0uq 215 18 of of IN cord-290231-4m9lj0uq 215 19 the the DT cord-290231-4m9lj0uq 215 20 E e NN cord-290231-4m9lj0uq 215 21 - - NN cord-290231-4m9lj0uq 215 22 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 215 23 of of IN cord-290231-4m9lj0uq 215 24 Murray Murray NNP cord-290231-4m9lj0uq 215 25 Valley Valley NNP cord-290231-4m9lj0uq 215 26 encephalitis encephalitis NN cord-290231-4m9lj0uq 215 27 virus virus NN cord-290231-4m9lj0uq 215 28 elicit elicit VBP cord-290231-4m9lj0uq 215 29 antiviral antiviral JJ cord-290231-4m9lj0uq 215 30 antibody antibody NN cord-290231-4m9lj0uq 215 31 Antibodies antibody NNS cord-290231-4m9lj0uq 215 32 to to IN cord-290231-4m9lj0uq 215 33 dengue dengue NN cord-290231-4m9lj0uq 215 34 2 2 CD cord-290231-4m9lj0uq 215 35 virus virus NN cord-290231-4m9lj0uq 215 36 E e NN cord-290231-4m9lj0uq 215 37 - - NN cord-290231-4m9lj0uq 215 38 glycoprotein glycoprotein JJ cord-290231-4m9lj0uq 215 39 synthetic synthetic JJ cord-290231-4m9lj0uq 215 40 peptides peptide NNS cord-290231-4m9lj0uq 215 41 identify identify VBP cord-290231-4m9lj0uq 215 42 antigenic antigenic JJ cord-290231-4m9lj0uq 215 43 conformation conformation NN cord-290231-4m9lj0uq 215 44 ldentiflcatton ldentiflcatton NN cord-290231-4m9lj0uq 215 45 of of IN cord-290231-4m9lj0uq 215 46 endoprotease endoprotease NNP cord-290231-4m9lj0uq 215 47 activity activity NN cord-290231-4m9lj0uq 215 48 in in IN cord-290231-4m9lj0uq 215 49 the the DT cord-290231-4m9lj0uq 215 50 rrans rrans NNP cord-290231-4m9lj0uq 215 51 Golgi Golgi NNP cord-290231-4m9lj0uq 215 52 membranes membrane NNS cord-290231-4m9lj0uq 215 53 of of IN cord-290231-4m9lj0uq 215 54 rat rat NN cord-290231-4m9lj0uq 215 55 liver liver NN cord-290231-4m9lj0uq 215 56 cells cell NNS cord-290231-4m9lj0uq 215 57 that that WDT cord-290231-4m9lj0uq 215 58 specifically specifically RB cord-290231-4m9lj0uq 215 59 processes process VBZ cord-290231-4m9lj0uq 215 60 in in IN cord-290231-4m9lj0uq 215 61 vitro vitro FW cord-290231-4m9lj0uq 215 62 the the DT cord-290231-4m9lj0uq 215 63 fusion fusion NN cord-290231-4m9lj0uq 215 64 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 215 65 precursor precursor NN cord-290231-4m9lj0uq 215 66 of of IN cord-290231-4m9lj0uq 215 67 virulent virulent JJ cord-290231-4m9lj0uq 215 68 Newcastle Newcastle NNP cord-290231-4m9lj0uq 215 69 disease disease NN cord-290231-4m9lj0uq 215 70 virus virus NN cord-290231-4m9lj0uq 215 71 Membrane membrane NN cord-290231-4m9lj0uq 215 72 fusion fusion NN cord-290231-4m9lj0uq 215 73 activity activity NN cord-290231-4m9lj0uq 215 74 of of IN cord-290231-4m9lj0uq 215 75 influenza influenza NN cord-290231-4m9lj0uq 215 76 virus virus NN cord-290231-4m9lj0uq 215 77 : : : cord-290231-4m9lj0uq 215 78 Effects effect NNS cord-290231-4m9lj0uq 215 79 of of IN cord-290231-4m9lj0uq 215 80 gangliosides ganglioside NNS cord-290231-4m9lj0uq 215 81 and and CC cord-290231-4m9lj0uq 215 82 negatively negatively RB cord-290231-4m9lj0uq 215 83 charged charge VBD cord-290231-4m9lj0uq 215 84 phospholipids phospholipid NNS cord-290231-4m9lj0uq 215 85 in in IN cord-290231-4m9lj0uq 215 86 target target NN cord-290231-4m9lj0uq 215 87 liposomes liposome NNS cord-290231-4m9lj0uq 215 88 Golgiigranule Golgiigranule NNP cord-290231-4m9lj0uq 215 89 processing processing NN cord-290231-4m9lj0uq 215 90 of of IN cord-290231-4m9lj0uq 215 91 peptide peptide JJ cord-290231-4m9lj0uq 215 92 hormone hormone NN cord-290231-4m9lj0uq 215 93 and and CC cord-290231-4m9lj0uq 215 94 neuropeptide neuropeptide NN cord-290231-4m9lj0uq 215 95 precursors precursor NNS cord-290231-4m9lj0uq 215 96 : : : cord-290231-4m9lj0uq 216 1 A a DT cord-290231-4m9lj0uq 216 2 minireview minireview NN cord-290231-4m9lj0uq 217 1 Proteolytic proteolytic JJ cord-290231-4m9lj0uq 217 2 cleavage cleavage NN cord-290231-4m9lj0uq 217 3 of of IN cord-290231-4m9lj0uq 217 4 peplomeric peplomeric JJ cord-290231-4m9lj0uq 217 5 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 217 6 E2 E2 NNP cord-290231-4m9lj0uq 217 7 of of IN cord-290231-4m9lj0uq 217 8 MHV MHV NNP cord-290231-4m9lj0uq 217 9 yields yield VBZ cord-290231-4m9lj0uq 217 10 two two CD cord-290231-4m9lj0uq 217 11 90 90 CD cord-290231-4m9lj0uq 217 12 K k NN cord-290231-4m9lj0uq 217 13 subunits subunit NNS cord-290231-4m9lj0uq 217 14 and and CC cord-290231-4m9lj0uq 217 15 activates activate VBZ cord-290231-4m9lj0uq 217 16 cell cell NN cord-290231-4m9lj0uq 217 17 fusion fusion NN cord-290231-4m9lj0uq 217 18 Proteolytic proteolytic JJ cord-290231-4m9lj0uq 217 19 cleavage cleavage NN cord-290231-4m9lj0uq 217 20 of of IN cord-290231-4m9lj0uq 217 21 the the DT cord-290231-4m9lj0uq 217 22 E2 E2 NNP cord-290231-4m9lj0uq 217 23 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 217 24 of of IN cord-290231-4m9lj0uq 217 25 murine murine JJ cord-290231-4m9lj0uq 217 26 coronavirus coronavirus NN cord-290231-4m9lj0uq 217 27 : : : cord-290231-4m9lj0uq 217 28 Activation activation NN cord-290231-4m9lj0uq 217 29 of of IN cord-290231-4m9lj0uq 217 30 cell cell NN cord-290231-4m9lj0uq 217 31 - - HYPH cord-290231-4m9lj0uq 217 32 fusing fuse VBG cord-290231-4m9lj0uq 217 33 activity activity NN cord-290231-4m9lj0uq 217 34 of of IN cord-290231-4m9lj0uq 217 35 virions virion NNS cord-290231-4m9lj0uq 217 36 by by IN cord-290231-4m9lj0uq 217 37 trypsin trypsin NN cord-290231-4m9lj0uq 217 38 and and CC cord-290231-4m9lj0uq 217 39 separation separation NN cord-290231-4m9lj0uq 217 40 of of IN cord-290231-4m9lj0uq 217 41 two two CD cord-290231-4m9lj0uq 217 42 different different JJ cord-290231-4m9lj0uq 217 43 90 90 CD cord-290231-4m9lj0uq 217 44 K k NN cord-290231-4m9lj0uq 217 45 cleavage cleavage NN cord-290231-4m9lj0uq 217 46 fragments fragment NNS cord-290231-4m9lj0uq 217 47 Electrophoretic electrophoretic JJ cord-290231-4m9lj0uq 217 48 transfer transfer NN cord-290231-4m9lj0uq 217 49 of of IN cord-290231-4m9lj0uq 217 50 proteins protein NNS cord-290231-4m9lj0uq 217 51 from from IN cord-290231-4m9lj0uq 217 52 polyacrylamide polyacrylamide JJ cord-290231-4m9lj0uq 217 53 gels gel NNS cord-290231-4m9lj0uq 217 54 to to IN cord-290231-4m9lj0uq 217 55 nitrocellulose nitrocellulose NN cord-290231-4m9lj0uq 217 56 sheets sheet NNS cord-290231-4m9lj0uq 217 57 : : : cord-290231-4m9lj0uq 217 58 Procedure procedure NN cord-290231-4m9lj0uq 217 59 and and CC cord-290231-4m9lj0uq 217 60 some some DT cord-290231-4m9lj0uq 217 61 applications application NNS cord-290231-4m9lj0uq 218 1 The the DT cord-290231-4m9lj0uq 218 2 heterodimerit heterodimerit NNP cord-290231-4m9lj0uq 218 3 association association NN cord-290231-4m9lj0uq 218 4 between between IN cord-290231-4m9lj0uq 218 5 the the DT cord-290231-4m9lj0uq 218 6 membrane membrane NN cord-290231-4m9lj0uq 218 7 proteins protein NNS cord-290231-4m9lj0uq 218 8 of of IN cord-290231-4m9lj0uq 218 9 Semliki Semliki NNP cord-290231-4m9lj0uq 218 10 Forest Forest NNP cord-290231-4m9lj0uq 218 11 virus virus NN cord-290231-4m9lj0uq 218 12 changes change VBZ cord-290231-4m9lj0uq 218 13 its -PRON- PRP$ cord-290231-4m9lj0uq 218 14 sensitivity sensitivity NN cord-290231-4m9lj0uq 218 15 to to IN cord-290231-4m9lj0uq 218 16 low low JJ cord-290231-4m9lj0uq 218 17 pH ph NN cord-290231-4m9lj0uq 218 18 during during IN cord-290231-4m9lj0uq 218 19 virus virus NN cord-290231-4m9lj0uq 218 20 maturation maturation NN cord-290231-4m9lj0uq 218 21 Cell cell NN cord-290231-4m9lj0uq 218 22 - - HYPH cord-290231-4m9lj0uq 218 23 associated associate VBN cord-290231-4m9lj0uq 218 24 West West NNP cord-290231-4m9lj0uq 218 25 Nile Nile NNP cord-290231-4m9lj0uq 218 26 flavivirus flavivirus NNP cord-290231-4m9lj0uq 218 27 is be VBZ cord-290231-4m9lj0uq 218 28 covered cover VBN cord-290231-4m9lj0uq 218 29 with with IN cord-290231-4m9lj0uq 219 1 E e LS cord-290231-4m9lj0uq 219 2 + + CC cord-290231-4m9lj0uq 219 3 Pre Pre NNP cord-290231-4m9lj0uq 219 4 - - JJ cord-290231-4m9lj0uq 219 5 M M NNP cord-290231-4m9lj0uq 219 6 protein protein NN cord-290231-4m9lj0uq 219 7 heterodimers heterodimer NNS cord-290231-4m9lj0uq 219 8 which which WDT cord-290231-4m9lj0uq 219 9 are be VBP cord-290231-4m9lj0uq 219 10 destroyed destroy VBN cord-290231-4m9lj0uq 219 11 and and CC cord-290231-4m9lj0uq 219 12 reorganized reorganize VBN cord-290231-4m9lj0uq 219 13 by by IN cord-290231-4m9lj0uq 219 14 proteolytic proteolytic JJ cord-290231-4m9lj0uq 219 15 cleavage cleavage NN cord-290231-4m9lj0uq 219 16 during during IN cord-290231-4m9lj0uq 219 17 virus virus NN cord-290231-4m9lj0uq 219 18 release release NN cord-290231-4m9lj0uq 219 19 Analysis Analysis NNP cord-290231-4m9lj0uq 219 20 of of IN cord-290231-4m9lj0uq 219 21 the the DT cord-290231-4m9lj0uq 219 22 influence influence NN cord-290231-4m9lj0uq 219 23 of of IN cord-290231-4m9lj0uq 219 24 proteolytic proteolytic JJ cord-290231-4m9lj0uq 219 25 cleavage cleavage NN cord-290231-4m9lj0uq 219 26 on on IN cord-290231-4m9lj0uq 219 27 the the DT cord-290231-4m9lj0uq 219 28 structural structural JJ cord-290231-4m9lj0uq 219 29 organization organization NN cord-290231-4m9lj0uq 219 30 of of IN cord-290231-4m9lj0uq 219 31 the the DT cord-290231-4m9lj0uq 219 32 surface surface NN cord-290231-4m9lj0uq 219 33 of of IN cord-290231-4m9lj0uq 219 34 the the DT cord-290231-4m9lj0uq 219 35 West West NNP cord-290231-4m9lj0uq 219 36 Nile Nile NNP cord-290231-4m9lj0uq 219 37 flavivirus flavivirus NNP cord-290231-4m9lj0uq 219 38 leads lead VBZ cord-290231-4m9lj0uq 219 39 to to IN cord-290231-4m9lj0uq 219 40 the the DT cord-290231-4m9lj0uq 219 41 isolation isolation NN cord-290231-4m9lj0uq 219 42 of of IN cord-290231-4m9lj0uq 219 43 a a DT cord-290231-4m9lj0uq 219 44 protease protease NN cord-290231-4m9lj0uq 219 45 - - HYPH cord-290231-4m9lj0uq 219 46 resistant resistant JJ cord-290231-4m9lj0uq 219 47 E e NN cord-290231-4m9lj0uq 219 48 protein protein NN cord-290231-4m9lj0uq 219 49 oligomer oligomer NNP cord-290231-4m9lj0uq 219 50 from from IN cord-290231-4m9lj0uq 219 51 the the DT cord-290231-4m9lj0uq 219 52 viral viral JJ cord-290231-4m9lj0uq 219 53 surface surface NN cord-290231-4m9lj0uq 219 54 Flavivirus Flavivirus NNP cord-290231-4m9lj0uq 219 55 replication replication NN cord-290231-4m9lj0uq 219 56 strategy strategy NN cord-290231-4m9lj0uq 219 57 Hemagglutinin Hemagglutinin NNP cord-290231-4m9lj0uq 219 58 of of IN cord-290231-4m9lj0uq 219 59 influenza influenza NN cord-290231-4m9lj0uq 219 60 virus virus NN cord-290231-4m9lj0uq 219 61 expressed express VBN cord-290231-4m9lj0uq 219 62 from from IN cord-290231-4m9lj0uq 219 63 a a DT cord-290231-4m9lj0uq 219 64 cloned clone VBN cord-290231-4m9lj0uq 219 65 gene gene NN cord-290231-4m9lj0uq 219 66 promotes promote VBZ cord-290231-4m9lj0uq 219 67 membrane membrane NN cord-290231-4m9lj0uq 219 68 fusion fusion NN cord-290231-4m9lj0uq 219 69 Membrane membrane NN cord-290231-4m9lj0uq 219 70 fusion fusion NN cord-290231-4m9lj0uq 219 71 proteins protein NNS cord-290231-4m9lj0uq 219 72 of of IN cord-290231-4m9lj0uq 219 73 enveloped envelop VBN cord-290231-4m9lj0uq 219 74 animal animal NN cord-290231-4m9lj0uq 219 75 viruses virus NNS cord-290231-4m9lj0uq 220 1 The the DT cord-290231-4m9lj0uq 220 2 structure structure NN cord-290231-4m9lj0uq 220 3 and and CC cord-290231-4m9lj0uq 220 4 function function NN cord-290231-4m9lj0uq 220 5 of of IN cord-290231-4m9lj0uq 220 6 the the DT cord-290231-4m9lj0uq 220 7 hemagglutinin hemagglutinin NN cord-290231-4m9lj0uq 220 8 membrane membrane NN cord-290231-4m9lj0uq 220 9 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 220 10 of of IN cord-290231-4m9lj0uq 220 11 influenza influenza NN cord-290231-4m9lj0uq 220 12 virus virus NN cord-290231-4m9lj0uq 220 13 Mutations Mutations NNPS cord-290231-4m9lj0uq 220 14 within within IN cord-290231-4m9lj0uq 220 15 the the DT cord-290231-4m9lj0uq 220 16 human human JJ cord-290231-4m9lj0uq 220 17 immunodeficiency immunodeficiency NN cord-290231-4m9lj0uq 220 18 virus virus NN cord-290231-4m9lj0uq 220 19 type type NN cord-290231-4m9lj0uq 220 20 1 1 CD cord-290231-4m9lj0uq 220 21 gpl60 gpl60 ADD cord-290231-4m9lj0uq 220 22 envelope envelope NN cord-290231-4m9lj0uq 220 23 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 220 24 alter alter VB cord-290231-4m9lj0uq 220 25 its -PRON- PRP$ cord-290231-4m9lj0uq 220 26 intracellular intracellular JJ cord-290231-4m9lj0uq 220 27 transport transport NN cord-290231-4m9lj0uq 220 28 and and CC cord-290231-4m9lj0uq 220 29 processing processing NN cord-290231-4m9lj0uq 220 30 Studies study NNS cord-290231-4m9lj0uq 220 31 on on IN cord-290231-4m9lj0uq 220 32 the the DT cord-290231-4m9lj0uq 220 33 glycosylation glycosylation NN cord-290231-4m9lj0uq 220 34 of of IN cord-290231-4m9lj0uq 220 35 flavivirus flavivirus JJ cord-290231-4m9lj0uq 220 36 E e NN cord-290231-4m9lj0uq 220 37 proteins protein NNS cord-290231-4m9lj0uq 220 38 and and CC cord-290231-4m9lj0uq 220 39 the the DT cord-290231-4m9lj0uq 220 40 role role NN cord-290231-4m9lj0uq 220 41 of of IN cord-290231-4m9lj0uq 220 42 carbohydrate carbohydrate NN cord-290231-4m9lj0uq 220 43 in in IN cord-290231-4m9lj0uq 220 44 antigenic antigenic JJ cord-290231-4m9lj0uq 220 45 structure structure NN cord-290231-4m9lj0uq 220 46 Expression expression NN cord-290231-4m9lj0uq 220 47 of of IN cord-290231-4m9lj0uq 220 48 a a DT cord-290231-4m9lj0uq 220 49 human human JJ cord-290231-4m9lj0uq 220 50 proprotein proprotein NNP cord-290231-4m9lj0uq 220 51 processing processing NN cord-290231-4m9lj0uq 220 52 enzyme enzyme NNP cord-290231-4m9lj0uq 220 53 : : : cord-290231-4m9lj0uq 221 1 Correct correct JJ cord-290231-4m9lj0uq 221 2 cleavage cleavage NN cord-290231-4m9lj0uq 221 3 of of IN cord-290231-4m9lj0uq 221 4 the the DT cord-290231-4m9lj0uq 221 5 von von NNP cord-290231-4m9lj0uq 221 6 Wilebrand Wilebrand NNP cord-290231-4m9lj0uq 221 7 factor factor NN cord-290231-4m9lj0uq 221 8 precursor precursor NN cord-290231-4m9lj0uq 221 9 at at IN cord-290231-4m9lj0uq 221 10 a a DT cord-290231-4m9lj0uq 221 11 paired pair VBN cord-290231-4m9lj0uq 221 12 basic basic JJ cord-290231-4m9lj0uq 221 13 amino amino NN cord-290231-4m9lj0uq 221 14 acid acid NN cord-290231-4m9lj0uq 221 15 site site NN cord-290231-4m9lj0uq 221 16 Induction induction NN cord-290231-4m9lj0uq 221 17 of of IN cord-290231-4m9lj0uq 221 18 protective protective JJ cord-290231-4m9lj0uq 221 19 immunity immunity NN cord-290231-4m9lj0uq 221 20 in in IN cord-290231-4m9lj0uq 221 21 animals animal NNS cord-290231-4m9lj0uq 221 22 vaccinated vaccinate VBN cord-290231-4m9lj0uq 221 23 with with IN cord-290231-4m9lj0uq 221 24 recombinant recombinant JJ cord-290231-4m9lj0uq 221 25 vaccinia vaccinia NN cord-290231-4m9lj0uq 221 26 viruses virus NNS cord-290231-4m9lj0uq 221 27 that that WDT cord-290231-4m9lj0uq 221 28 express express VBP cord-290231-4m9lj0uq 221 29 PreM PreM NNP cord-290231-4m9lj0uq 221 30 and and CC cord-290231-4m9lj0uq 221 31 E e NN cord-290231-4m9lj0uq 221 32 glycoproteins glycoprotein NNS cord-290231-4m9lj0uq 221 33 of of IN cord-290231-4m9lj0uq 221 34 Japanese japanese JJ cord-290231-4m9lj0uq 221 35 encephalitis encephalitis RB cord-290231-4m9lj0uq 221 36 virus virus NN cord-290231-4m9lj0uq 222 1 We -PRON- PRP cord-290231-4m9lj0uq 222 2 thank thank VBP cord-290231-4m9lj0uq 222 3 Craig Craig NNP cord-290231-4m9lj0uq 222 4 Miles Miles NNP cord-290231-4m9lj0uq 222 5 and and CC cord-290231-4m9lj0uq 222 6 Cindy Cindy NNP cord-290231-4m9lj0uq 222 7 Bozic Bozic NNP cord-290231-4m9lj0uq 222 8 of of IN cord-290231-4m9lj0uq 222 9 Macromolecular Macromolecular NNP cord-290231-4m9lj0uq 222 10 Resources Resources NNPS cord-290231-4m9lj0uq 222 11 , , , cord-290231-4m9lj0uq 222 12 Colorado Colorado NNP cord-290231-4m9lj0uq 222 13 State State NNP cord-290231-4m9lj0uq 222 14 University University NNP cord-290231-4m9lj0uq 222 15 , , , cord-290231-4m9lj0uq 222 16 and and CC cord-290231-4m9lj0uq 222 17 Danny Danny NNP cord-290231-4m9lj0uq 222 18 Jue Jue NNP cord-290231-4m9lj0uq 222 19 and and CC cord-290231-4m9lj0uq 222 20 Tamara Tamara NNP cord-290231-4m9lj0uq 222 21 Hine Hine NNP cord-290231-4m9lj0uq 222 22 of of IN cord-290231-4m9lj0uq 222 23 the the DT cord-290231-4m9lj0uq 222 24 Biotechnology Biotechnology NNP cord-290231-4m9lj0uq 222 25 Core Core NNP cord-290231-4m9lj0uq 222 26 Facility Facility NNP cord-290231-4m9lj0uq 222 27 , , , cord-290231-4m9lj0uq 222 28 Centers Centers NNPS cord-290231-4m9lj0uq 222 29 for for IN cord-290231-4m9lj0uq 222 30 Disease Disease NNP cord-290231-4m9lj0uq 222 31 Control Control NNP cord-290231-4m9lj0uq 222 32 , , , cord-290231-4m9lj0uq 222 33 for for IN cord-290231-4m9lj0uq 222 34 amino amino NN cord-290231-4m9lj0uq 222 35 - - HYPH cord-290231-4m9lj0uq 222 36 terminal terminal NN cord-290231-4m9lj0uq 222 37 sequencing sequencing NN cord-290231-4m9lj0uq 222 38 of of IN cord-290231-4m9lj0uq 222 39 the the DT cord-290231-4m9lj0uq 222 40 enzymatically enzymatically RB cord-290231-4m9lj0uq 222 41 derived derive VBN cord-290231-4m9lj0uq 222 42 E e NN cord-290231-4m9lj0uq 222 43 glycoprotein glycoprotein NN cord-290231-4m9lj0uq 222 44 fragments fragment NNS cord-290231-4m9lj0uq 222 45 . . .