id sid tid token lemma pos cord-291611-cfe8yujp 1 1 key key NN cord-291611-cfe8yujp 1 2 : : : cord-291611-cfe8yujp 2 1 cord-291611-cfe8yujp cord-291611-cfe8yujp NNP cord-291611-cfe8yujp 2 2 authors author NNS cord-291611-cfe8yujp 2 3 : : : cord-291611-cfe8yujp 2 4 Zhang Zhang NNP cord-291611-cfe8yujp 2 5 , , , cord-291611-cfe8yujp 2 6 Xuming Xuming NNP cord-291611-cfe8yujp 2 7 ; ; : cord-291611-cfe8yujp 2 8 Kousoulas Kousoulas NNP cord-291611-cfe8yujp 2 9 , , , cord-291611-cfe8yujp 2 10 Konstantin Konstantin NNP cord-291611-cfe8yujp 2 11 G. G. NNP cord-291611-cfe8yujp 2 12 ; ; : cord-291611-cfe8yujp 2 13 Storz Storz NNP cord-291611-cfe8yujp 2 14 , , , cord-291611-cfe8yujp 2 15 Johannes Johannes NNP cord-291611-cfe8yujp 2 16 title title NN cord-291611-cfe8yujp 2 17 : : : cord-291611-cfe8yujp 3 1 Comparison comparison NN cord-291611-cfe8yujp 3 2 of of IN cord-291611-cfe8yujp 3 3 the the DT cord-291611-cfe8yujp 3 4 nucleotide nucleotide JJ cord-291611-cfe8yujp 3 5 and and CC cord-291611-cfe8yujp 3 6 deduced deduce VBN cord-291611-cfe8yujp 3 7 amino amino NN cord-291611-cfe8yujp 3 8 acid acid NN cord-291611-cfe8yujp 3 9 sequences sequence NNS cord-291611-cfe8yujp 3 10 of of IN cord-291611-cfe8yujp 3 11 the the DT cord-291611-cfe8yujp 3 12 S S NNP cord-291611-cfe8yujp 3 13 genes gene NNS cord-291611-cfe8yujp 3 14 specified specify VBN cord-291611-cfe8yujp 3 15 by by IN cord-291611-cfe8yujp 3 16 virulent virulent JJ cord-291611-cfe8yujp 3 17 and and CC cord-291611-cfe8yujp 3 18 avirulent avirulent JJ cord-291611-cfe8yujp 3 19 strains strain NNS cord-291611-cfe8yujp 3 20 of of IN cord-291611-cfe8yujp 3 21 bovine bovine JJ cord-291611-cfe8yujp 3 22 coronaviruses coronaviruse NNS cord-291611-cfe8yujp 3 23 date date NN cord-291611-cfe8yujp 3 24 : : : cord-291611-cfe8yujp 3 25 1991 1991 CD cord-291611-cfe8yujp 3 26 - - SYM cord-291611-cfe8yujp 3 27 07 07 CD cord-291611-cfe8yujp 3 28 - - HYPH cord-291611-cfe8yujp 3 29 31 31 CD cord-291611-cfe8yujp 3 30 journal journal NN cord-291611-cfe8yujp 3 31 : : : cord-291611-cfe8yujp 4 1 Virology virology NN cord-291611-cfe8yujp 4 2 DOI DOI NNP cord-291611-cfe8yujp 4 3 : : : cord-291611-cfe8yujp 4 4 10.1016/0042 10.1016/0042 CD cord-291611-cfe8yujp 4 5 - - HYPH cord-291611-cfe8yujp 4 6 6822(91)90154 6822(91)90154 CD cord-291611-cfe8yujp 4 7 - - HYPH cord-291611-cfe8yujp 4 8 4 4 CD cord-291611-cfe8yujp 5 1 sha sha NNP cord-291611-cfe8yujp 5 2 : : : cord-291611-cfe8yujp 6 1 9d4c78d5141300b335620c2f5e2365b75c925ecc 9d4c78d5141300b335620c2f5e2365b75c925ecc JJ cord-291611-cfe8yujp 6 2 doc_id doc_id NNPS cord-291611-cfe8yujp 6 3 : : : cord-291611-cfe8yujp 6 4 291611 291611 CD cord-291611-cfe8yujp 6 5 cord_uid cord_uid NNS cord-291611-cfe8yujp 6 6 : : : cord-291611-cfe8yujp 7 1 cfe8yujp cfe8yujp NNP cord-291611-cfe8yujp 7 2 Abstract Abstract NNP cord-291611-cfe8yujp 8 1 The the DT cord-291611-cfe8yujp 8 2 entire entire JJ cord-291611-cfe8yujp 8 3 nucleotide nucleotide JJ cord-291611-cfe8yujp 8 4 sequences sequence NNS cord-291611-cfe8yujp 8 5 of of IN cord-291611-cfe8yujp 8 6 the the DT cord-291611-cfe8yujp 8 7 spike spike NN cord-291611-cfe8yujp 8 8 glycoprotein glycoprotein NN cord-291611-cfe8yujp 8 9 ( ( -LRB- cord-291611-cfe8yujp 8 10 S S NNP cord-291611-cfe8yujp 8 11 ) ) -RRB- cord-291611-cfe8yujp 8 12 genes gene NNS cord-291611-cfe8yujp 8 13 of of IN cord-291611-cfe8yujp 8 14 the the DT cord-291611-cfe8yujp 8 15 highly highly RB cord-291611-cfe8yujp 8 16 virulent virulent JJ cord-291611-cfe8yujp 8 17 bovine bovine JJ cord-291611-cfe8yujp 8 18 coronavirus coronavirus NN cord-291611-cfe8yujp 8 19 ( ( -LRB- cord-291611-cfe8yujp 8 20 BCV BCV NNP cord-291611-cfe8yujp 8 21 ) ) -RRB- cord-291611-cfe8yujp 8 22 strain strain VBP cord-291611-cfe8yujp 8 23 BCV BCV NNP cord-291611-cfe8yujp 8 24 - - HYPH cord-291611-cfe8yujp 8 25 LY138 LY138 NNP cord-291611-cfe8yujp 8 26 , , , cord-291611-cfe8yujp 8 27 the the DT cord-291611-cfe8yujp 8 28 avirulent avirulent NN cord-291611-cfe8yujp 8 29 BCV BCV NNP cord-291611-cfe8yujp 8 30 - - HYPH cord-291611-cfe8yujp 8 31 L9 L9 NNP cord-291611-cfe8yujp 8 32 and and CC cord-291611-cfe8yujp 8 33 related relate VBN cord-291611-cfe8yujp 8 34 Norden Norden NNP cord-291611-cfe8yujp 8 35 Vaccine Vaccine NNP cord-291611-cfe8yujp 8 36 ( ( -LRB- cord-291611-cfe8yujp 8 37 BCV BCV NNP cord-291611-cfe8yujp 8 38 - - HYPH cord-291611-cfe8yujp 8 39 Vaccine Vaccine NNP cord-291611-cfe8yujp 8 40 ) ) -RRB- cord-291611-cfe8yujp 8 41 strains strain NNS cord-291611-cfe8yujp 8 42 were be VBD cord-291611-cfe8yujp 8 43 determined determine VBN cord-291611-cfe8yujp 8 44 using use VBG cord-291611-cfe8yujp 8 45 the the DT cord-291611-cfe8yujp 8 46 polymerase polymerase NN cord-291611-cfe8yujp 8 47 chain chain NN cord-291611-cfe8yujp 8 48 reaction reaction NN cord-291611-cfe8yujp 8 49 ( ( -LRB- cord-291611-cfe8yujp 8 50 PCR PCR NNP cord-291611-cfe8yujp 8 51 ) ) -RRB- cord-291611-cfe8yujp 8 52 to to TO cord-291611-cfe8yujp 8 53 amplify amplify VB cord-291611-cfe8yujp 8 54 cDNAs cdna NNS cord-291611-cfe8yujp 8 55 obtained obtain VBN cord-291611-cfe8yujp 8 56 by by IN cord-291611-cfe8yujp 8 57 reverse reverse JJ cord-291611-cfe8yujp 8 58 transcription transcription NN cord-291611-cfe8yujp 8 59 of of IN cord-291611-cfe8yujp 8 60 viral viral JJ cord-291611-cfe8yujp 8 61 RNA RNA NNP cord-291611-cfe8yujp 8 62 , , , cord-291611-cfe8yujp 8 63 and and CC cord-291611-cfe8yujp 8 64 to to TO cord-291611-cfe8yujp 8 65 produce produce VB cord-291611-cfe8yujp 8 66 single single JJ cord-291611-cfe8yujp 8 67 strand strand NN cord-291611-cfe8yujp 8 68 cDNAs cdna NNS cord-291611-cfe8yujp 8 69 for for IN cord-291611-cfe8yujp 8 70 DNA dna NN cord-291611-cfe8yujp 8 71 sequencing sequencing NN cord-291611-cfe8yujp 8 72 . . . cord-291611-cfe8yujp 9 1 The the DT cord-291611-cfe8yujp 9 2 S S NNP cord-291611-cfe8yujp 9 3 gene gene NN cord-291611-cfe8yujp 9 4 sequences sequence NNS cord-291611-cfe8yujp 9 5 of of IN cord-291611-cfe8yujp 9 6 these these DT cord-291611-cfe8yujp 9 7 viral viral JJ cord-291611-cfe8yujp 9 8 strains strain NNS cord-291611-cfe8yujp 9 9 were be VBD cord-291611-cfe8yujp 9 10 compared compare VBN cord-291611-cfe8yujp 9 11 with with IN cord-291611-cfe8yujp 9 12 those those DT cord-291611-cfe8yujp 9 13 of of IN cord-291611-cfe8yujp 9 14 recently recently RB cord-291611-cfe8yujp 9 15 published publish VBN cord-291611-cfe8yujp 9 16 strains strain NNS cord-291611-cfe8yujp 9 17 BCV BCV NNP cord-291611-cfe8yujp 9 18 - - HYPH cord-291611-cfe8yujp 9 19 Mebus Mebus NNP cord-291611-cfe8yujp 9 20 , , , cord-291611-cfe8yujp 9 21 BCV BCV NNP cord-291611-cfe8yujp 9 22 - - HYPH cord-291611-cfe8yujp 9 23 Quebec Quebec NNP cord-291611-cfe8yujp 9 24 , , , cord-291611-cfe8yujp 9 25 and and CC cord-291611-cfe8yujp 9 26 BCV BCV NNP cord-291611-cfe8yujp 9 27 - - HYPH cord-291611-cfe8yujp 9 28 F15 F15 NNP cord-291611-cfe8yujp 9 29 . . . cord-291611-cfe8yujp 10 1 An an DT cord-291611-cfe8yujp 10 2 open open JJ cord-291611-cfe8yujp 10 3 reading reading NN cord-291611-cfe8yujp 10 4 frame frame NN cord-291611-cfe8yujp 10 5 of of IN cord-291611-cfe8yujp 10 6 4092 4092 CD cord-291611-cfe8yujp 10 7 nucleotides nucleotide NNS cord-291611-cfe8yujp 10 8 , , , cord-291611-cfe8yujp 10 9 encoding encode VBG cord-291611-cfe8yujp 10 10 a a DT cord-291611-cfe8yujp 10 11 protein protein NN cord-291611-cfe8yujp 10 12 of of IN cord-291611-cfe8yujp 10 13 1363 1363 CD cord-291611-cfe8yujp 10 14 amino amino NN cord-291611-cfe8yujp 10 15 acid acid NN cord-291611-cfe8yujp 10 16 residues residue NNS cord-291611-cfe8yujp 10 17 , , , cord-291611-cfe8yujp 10 18 was be VBD cord-291611-cfe8yujp 10 19 found find VBN cord-291611-cfe8yujp 10 20 in in IN cord-291611-cfe8yujp 10 21 all all DT cord-291611-cfe8yujp 10 22 six six CD cord-291611-cfe8yujp 10 23 strains strain NNS cord-291611-cfe8yujp 10 24 . . . cord-291611-cfe8yujp 11 1 Frameshifts Frameshifts NNP cord-291611-cfe8yujp 11 2 and and CC cord-291611-cfe8yujp 11 3 insertions insertion NNS cord-291611-cfe8yujp 11 4 or or CC cord-291611-cfe8yujp 11 5 deletions deletion NNS cord-291611-cfe8yujp 11 6 were be VBD cord-291611-cfe8yujp 11 7 not not RB cord-291611-cfe8yujp 11 8 observed observe VBN cord-291611-cfe8yujp 11 9 except except IN cord-291611-cfe8yujp 11 10 for for IN cord-291611-cfe8yujp 11 11 the the DT cord-291611-cfe8yujp 11 12 BCV BCV NNP cord-291611-cfe8yujp 11 13 - - HYPH cord-291611-cfe8yujp 11 14 Fl Fl NNP cord-291611-cfe8yujp 11 15 5 5 CD cord-291611-cfe8yujp 11 16 . . . cord-291611-cfe8yujp 12 1 The the DT cord-291611-cfe8yujp 12 2 S S NNP cord-291611-cfe8yujp 12 3 gene gene NN cord-291611-cfe8yujp 12 4 sequences sequence NNS cord-291611-cfe8yujp 12 5 were be VBD cord-291611-cfe8yujp 12 6 more more JJR cord-291611-cfe8yujp 12 7 than than IN cord-291611-cfe8yujp 12 8 98 98 CD cord-291611-cfe8yujp 12 9 % % NN cord-291611-cfe8yujp 12 10 conserved conserve VBN cord-291611-cfe8yujp 12 11 overall overall RB cord-291611-cfe8yujp 12 12 inspite inspite JJ cord-291611-cfe8yujp 12 13 of of IN cord-291611-cfe8yujp 12 14 different different JJ cord-291611-cfe8yujp 12 15 origins origin NNS cord-291611-cfe8yujp 12 16 of of IN cord-291611-cfe8yujp 12 17 the the DT cord-291611-cfe8yujp 12 18 six six CD cord-291611-cfe8yujp 12 19 viruses virus NNS cord-291611-cfe8yujp 12 20 . . . cord-291611-cfe8yujp 13 1 There there EX cord-291611-cfe8yujp 13 2 were be VBD cord-291611-cfe8yujp 13 3 45 45 CD cord-291611-cfe8yujp 13 4 to to TO cord-291611-cfe8yujp 13 5 56 56 CD cord-291611-cfe8yujp 13 6 nt nt RB cord-291611-cfe8yujp 13 7 differences difference NNS cord-291611-cfe8yujp 13 8 between between IN cord-291611-cfe8yujp 13 9 the the DT cord-291611-cfe8yujp 13 10 virulent virulent JJ cord-291611-cfe8yujp 13 11 and and CC cord-291611-cfe8yujp 13 12 avirulent avirulent JJ cord-291611-cfe8yujp 13 13 groups group NNS cord-291611-cfe8yujp 13 14 while while IN cord-291611-cfe8yujp 13 15 there there EX cord-291611-cfe8yujp 13 16 were be VBD cord-291611-cfe8yujp 13 17 6 6 CD cord-291611-cfe8yujp 13 18 to to TO cord-291611-cfe8yujp 13 19 14 14 CD cord-291611-cfe8yujp 13 20 nt nt RB cord-291611-cfe8yujp 13 21 differences difference NNS cord-291611-cfe8yujp 13 22 among among IN cord-291611-cfe8yujp 13 23 four four CD cord-291611-cfe8yujp 13 24 avirulent avirulent NN cord-291611-cfe8yujp 13 25 strains strain NNS cord-291611-cfe8yujp 13 26 . . . cord-291611-cfe8yujp 14 1 Comparison comparison NN cord-291611-cfe8yujp 14 2 of of IN cord-291611-cfe8yujp 14 3 the the DT cord-291611-cfe8yujp 14 4 deduced deduce VBN cord-291611-cfe8yujp 14 5 amino amino NN cord-291611-cfe8yujp 14 6 acid acid NN cord-291611-cfe8yujp 14 7 sequences sequence NNS cord-291611-cfe8yujp 14 8 indicated indicate VBD cord-291611-cfe8yujp 14 9 that that IN cord-291611-cfe8yujp 14 10 the the DT cord-291611-cfe8yujp 14 11 S S NNP cord-291611-cfe8yujp 14 12 proteins protein NNS cord-291611-cfe8yujp 14 13 had have VBD cord-291611-cfe8yujp 14 14 typical typical JJ cord-291611-cfe8yujp 14 15 properties property NNS cord-291611-cfe8yujp 14 16 of of IN cord-291611-cfe8yujp 14 17 membrane membrane NN cord-291611-cfe8yujp 14 18 glycoproteins glycoprotein NNS cord-291611-cfe8yujp 14 19 . . . cord-291611-cfe8yujp 15 1 Nineteen nineteen CD cord-291611-cfe8yujp 15 2 Winked wink VBD cord-291611-cfe8yujp 15 3 glycosylation glycosylation NN cord-291611-cfe8yujp 15 4 sites site NNS cord-291611-cfe8yujp 15 5 were be VBD cord-291611-cfe8yujp 15 6 predicted predict VBN cord-291611-cfe8yujp 15 7 in in IN cord-291611-cfe8yujp 15 8 five five CD cord-291611-cfe8yujp 15 9 strains strain NNS cord-291611-cfe8yujp 15 10 , , , cord-291611-cfe8yujp 15 11 and and CC cord-291611-cfe8yujp 15 12 18 18 CD cord-291611-cfe8yujp 15 13 of of IN cord-291611-cfe8yujp 15 14 them -PRON- PRP cord-291611-cfe8yujp 15 15 were be VBD cord-291611-cfe8yujp 15 16 conserved conserve VBN cord-291611-cfe8yujp 15 17 in in IN cord-291611-cfe8yujp 15 18 the the DT cord-291611-cfe8yujp 15 19 avirulent avirulent NN cord-291611-cfe8yujp 15 20 strain strain NN cord-291611-cfe8yujp 15 21 BCV BCV NNP cord-291611-cfe8yujp 15 22 - - HYPH cord-291611-cfe8yujp 15 23 L9 L9 NNP cord-291611-cfe8yujp 15 24 . . . cord-291611-cfe8yujp 16 1 The the DT cord-291611-cfe8yujp 16 2 sequence sequence NN cord-291611-cfe8yujp 16 3 KRRSRR KRRSRR NNP cord-291611-cfe8yujp 16 4 at at IN cord-291611-cfe8yujp 16 5 the the DT cord-291611-cfe8yujp 16 6 predicted predict VBN cord-291611-cfe8yujp 16 7 proteolytic proteolytic JJ cord-291611-cfe8yujp 16 8 cleavage cleavage NN cord-291611-cfe8yujp 16 9 site site NN cord-291611-cfe8yujp 16 10 was be VBD cord-291611-cfe8yujp 16 11 identified identify VBN cord-291611-cfe8yujp 16 12 in in IN cord-291611-cfe8yujp 16 13 five five CD cord-291611-cfe8yujp 16 14 strains strain NNS cord-291611-cfe8yujp 16 15 while while IN cord-291611-cfe8yujp 16 16 the the DT cord-291611-cfe8yujp 16 17 sequence sequence NN cord-291611-cfe8yujp 16 18 KRRSVR KRRSVR NNP cord-291611-cfe8yujp 16 19 was be VBD cord-291611-cfe8yujp 16 20 found find VBN cord-291611-cfe8yujp 16 21 in in IN cord-291611-cfe8yujp 16 22 BCV BCV NNP cord-291611-cfe8yujp 16 23 - - HYPH cord-291611-cfe8yujp 16 24 Fl Fl NNP cord-291611-cfe8yujp 16 25 5 5 CD cord-291611-cfe8yujp 16 26 . . . cord-291611-cfe8yujp 17 1 Substitutions substitution NNS cord-291611-cfe8yujp 17 2 of of IN cord-291611-cfe8yujp 17 3 few few JJ cord-291611-cfe8yujp 17 4 amino amino JJ cord-291611-cfe8yujp 17 5 acids acid NNS cord-291611-cfe8yujp 17 6 in in IN cord-291611-cfe8yujp 17 7 the the DT cord-291611-cfe8yujp 17 8 putative putative JJ cord-291611-cfe8yujp 17 9 fusogenic fusogenic JJ cord-291611-cfe8yujp 17 10 domains domain NNS cord-291611-cfe8yujp 17 11 and and CC cord-291611-cfe8yujp 17 12 two two CD cord-291611-cfe8yujp 17 13 prolines proline NNS cord-291611-cfe8yujp 17 14 at at IN cord-291611-cfe8yujp 17 15 507 507 CD cord-291611-cfe8yujp 17 16 and and CC cord-291611-cfe8yujp 17 17 567 567 CD cord-291611-cfe8yujp 17 18 in in IN cord-291611-cfe8yujp 17 19 the the DT cord-291611-cfe8yujp 17 20 antigenic antigenic JJ cord-291611-cfe8yujp 17 21 domains domain NNS cord-291611-cfe8yujp 17 22 may may MD cord-291611-cfe8yujp 17 23 cause cause VB cord-291611-cfe8yujp 17 24 altered alter VBN cord-291611-cfe8yujp 17 25 immunogenic immunogenic JJ cord-291611-cfe8yujp 17 26 and and CC cord-291611-cfe8yujp 17 27 other other JJ cord-291611-cfe8yujp 17 28 functional functional JJ cord-291611-cfe8yujp 17 29 properties property NNS cord-291611-cfe8yujp 17 30 of of IN cord-291611-cfe8yujp 17 31 the the DT cord-291611-cfe8yujp 17 32 S S NNP cord-291611-cfe8yujp 17 33 proteins protein NNS cord-291611-cfe8yujp 17 34 specified specify VBN cord-291611-cfe8yujp 17 35 by by IN cord-291611-cfe8yujp 17 36 the the DT cord-291611-cfe8yujp 17 37 virulent virulent JJ cord-291611-cfe8yujp 17 38 and and CC cord-291611-cfe8yujp 17 39 avirulent avirulent JJ cord-291611-cfe8yujp 17 40 BCV BCV NNP cord-291611-cfe8yujp 17 41 strains strain VBZ cord-291611-cfe8yujp 17 42 . . . cord-291611-cfe8yujp 18 1 Nine nine CD cord-291611-cfe8yujp 18 2 amino amino NN cord-291611-cfe8yujp 18 3 acid acid NN cord-291611-cfe8yujp 18 4 substitutions substitution NNS cord-291611-cfe8yujp 18 5 between between IN cord-291611-cfe8yujp 18 6 the the DT cord-291611-cfe8yujp 18 7 virulent virulent JJ cord-291611-cfe8yujp 18 8 and and CC cord-291611-cfe8yujp 18 9 avirulent avirulent JJ cord-291611-cfe8yujp 18 10 groups group NNS cord-291611-cfe8yujp 18 11 may may MD cord-291611-cfe8yujp 18 12 correlate correlate VB cord-291611-cfe8yujp 18 13 with with IN cord-291611-cfe8yujp 18 14 BCV BCV NNP cord-291611-cfe8yujp 18 15 virulence virulence NN cord-291611-cfe8yujp 18 16 . . . cord-291611-cfe8yujp 19 1 Bovine bovine JJ cord-291611-cfe8yujp 19 2 coronavirus coronavirus NN cord-291611-cfe8yujp 19 3 ( ( -LRB- cord-291611-cfe8yujp 19 4 BCV BCV NNP cord-291611-cfe8yujp 19 5 ) ) -RRB- cord-291611-cfe8yujp 19 6 is be VBZ cord-291611-cfe8yujp 19 7 a a DT cord-291611-cfe8yujp 19 8 member member NN cord-291611-cfe8yujp 19 9 of of IN cord-291611-cfe8yujp 19 10 coronaviridae coronaviridae NNP cord-291611-cfe8yujp 19 11 , , , cord-291611-cfe8yujp 19 12 causing cause VBG cord-291611-cfe8yujp 19 13 severe severe JJ cord-291611-cfe8yujp 19 14 diarrhea diarrhea NN cord-291611-cfe8yujp 19 15 in in IN cord-291611-cfe8yujp 19 16 newborn newborn JJ cord-291611-cfe8yujp 19 17 calves calf NNS cord-291611-cfe8yujp 19 18 and and CC cord-291611-cfe8yujp 19 19 winter winter NN cord-291611-cfe8yujp 19 20 dysentery dysentery NN cord-291611-cfe8yujp 19 21 in in IN cord-291611-cfe8yujp 19 22 adult adult NN cord-291611-cfe8yujp 19 23 cattle cattle NNS cord-291611-cfe8yujp 19 24 ( ( -LRB- cord-291611-cfe8yujp 19 25 1 1 CD cord-291611-cfe8yujp 19 26 , , , cord-291611-cfe8yujp 19 27 2 2 CD cord-291611-cfe8yujp 19 28 ) ) -RRB- cord-291611-cfe8yujp 19 29 . . . cord-291611-cfe8yujp 20 1 It -PRON- PRP cord-291611-cfe8yujp 20 2 possesses possess VBZ cord-291611-cfe8yujp 20 3 a a DT cord-291611-cfe8yujp 20 4 single single JJ cord-291611-cfe8yujp 20 5 - - HYPH cord-291611-cfe8yujp 20 6 stranded stranded JJ cord-291611-cfe8yujp 20 7 , , , cord-291611-cfe8yujp 20 8 nonsegmented nonsegmente VBD cord-291611-cfe8yujp 20 9 RNA RNA NNP cord-291611-cfe8yujp 20 10 genome genome NN cord-291611-cfe8yujp 20 11 with with IN cord-291611-cfe8yujp 20 12 positive positive JJ cord-291611-cfe8yujp 20 13 polarity polarity NN cord-291611-cfe8yujp 20 14 ( ( -LRB- cord-291611-cfe8yujp 20 15 3 3 CD cord-291611-cfe8yujp 20 16 ) ) -RRB- cord-291611-cfe8yujp 20 17 . . . cord-291611-cfe8yujp 21 1 The the DT cord-291611-cfe8yujp 21 2 virion virion NN cord-291611-cfe8yujp 21 3 contains contain VBZ cord-291611-cfe8yujp 21 4 four four CD cord-291611-cfe8yujp 21 5 major major JJ cord-291611-cfe8yujp 21 6 structural structural JJ cord-291611-cfe8yujp 21 7 proteins protein NNS cord-291611-cfe8yujp 21 8 : : : cord-291611-cfe8yujp 21 9 the the DT cord-291611-cfe8yujp 21 10 nucleocapsid nucleocapsid NNP cord-291611-cfe8yujp 21 11 protein protein NNP cord-291611-cfe8yujp 21 12 ( ( -LRB- cord-291611-cfe8yujp 21 13 N N NNP cord-291611-cfe8yujp 21 14 ) ) -RRB- cord-291611-cfe8yujp 21 15 , , , cord-291611-cfe8yujp 21 16 the the DT cord-291611-cfe8yujp 21 17 transmembrane transmembrane NNP cord-291611-cfe8yujp 21 18 protein protein NN cord-291611-cfe8yujp 21 19 ( ( -LRB- cord-291611-cfe8yujp 21 20 M M NNP cord-291611-cfe8yujp 21 21 ) ) -RRB- cord-291611-cfe8yujp 21 22 , , , cord-291611-cfe8yujp 21 23 the the DT cord-291611-cfe8yujp 21 24 hemagglutinin/ hemagglutinin/ CD cord-291611-cfe8yujp 21 25 esterase esterase NN cord-291611-cfe8yujp 21 26 protein protein NN cord-291611-cfe8yujp 21 27 ( ( -LRB- cord-291611-cfe8yujp 21 28 HE HE NNP cord-291611-cfe8yujp 21 29 ) ) -RRB- cord-291611-cfe8yujp 21 30 and and CC cord-291611-cfe8yujp 21 31 the the DT cord-291611-cfe8yujp 21 32 spike spike NN cord-291611-cfe8yujp 21 33 protein protein NN cord-291611-cfe8yujp 21 34 ( ( -LRB- cord-291611-cfe8yujp 21 35 S s NN cord-291611-cfe8yujp 21 36 ) ) -RRB- cord-291611-cfe8yujp 21 37 ( ( -LRB- cord-291611-cfe8yujp 21 38 4 4 CD cord-291611-cfe8yujp 21 39 ) ) -RRB- cord-291611-cfe8yujp 21 40 . . . cord-291611-cfe8yujp 22 1 The the DT cord-291611-cfe8yujp 22 2 S S NNP cord-291611-cfe8yujp 22 3 glycoprotein glycoprotein NN cord-291611-cfe8yujp 22 4 is be VBZ cord-291611-cfe8yujp 22 5 a a DT cord-291611-cfe8yujp 22 6 predominant predominant JJ cord-291611-cfe8yujp 22 7 peplomeric peplomeric JJ cord-291611-cfe8yujp 22 8 structure structure NN cord-291611-cfe8yujp 22 9 forming form VBG cord-291611-cfe8yujp 22 10 the the DT cord-291611-cfe8yujp 22 11 typical typical JJ cord-291611-cfe8yujp 22 12 coronavirus coronavirus NN cord-291611-cfe8yujp 22 13 morphology morphology NN cord-291611-cfe8yujp 22 14 . . . cord-291611-cfe8yujp 23 1 It -PRON- PRP cord-291611-cfe8yujp 23 2 is be VBZ cord-291611-cfe8yujp 23 3 synthesized synthesize VBN cord-291611-cfe8yujp 23 4 as as IN cord-291611-cfe8yujp 23 5 a a DT cord-291611-cfe8yujp 23 6 high high JJ cord-291611-cfe8yujp 23 7 molecular molecular JJ cord-291611-cfe8yujp 23 8 weight weight NN cord-291611-cfe8yujp 23 9 ( ( -LRB- cord-291611-cfe8yujp 23 10 mol mol NNP cord-291611-cfe8yujp 23 11 . . . cord-291611-cfe8yujp 23 12 wt wt NNP cord-291611-cfe8yujp 23 13 . . . cord-291611-cfe8yujp 23 14 ) ) -RRB- cord-291611-cfe8yujp 23 15 precursor precursor NN cord-291611-cfe8yujp 23 16 ( ( -LRB- cord-291611-cfe8yujp 23 17 gpl90 gpl90 NN cord-291611-cfe8yujp 23 18 ) ) -RRB- cord-291611-cfe8yujp 23 19 which which WDT cord-291611-cfe8yujp 23 20 is be VBZ cord-291611-cfe8yujp 23 21 cleaved cleave VBN cord-291611-cfe8yujp 23 22 to to TO cord-291611-cfe8yujp 23 23 yield yield VB cord-291611-cfe8yujp 23 24 two two CD cord-291611-cfe8yujp 23 25 comigrating comigrate VBG cord-291611-cfe8yujp 23 26 subunit subunit NN cord-291611-cfe8yujp 23 27 polypeptides polypeptide NNS cord-291611-cfe8yujp 23 28 : : : cord-291611-cfe8yujp 23 29 the the DT cord-291611-cfe8yujp 23 30 N N NNP cord-291611-cfe8yujp 23 31 - - HYPH cord-291611-cfe8yujp 23 32 terminal terminal JJ cord-291611-cfe8yujp 23 33 half half NN cord-291611-cfe8yujp 23 34 ( ( -LRB- cord-291611-cfe8yujp 23 35 Sl Sl NNP cord-291611-cfe8yujp 23 36 ) ) -RRB- cord-291611-cfe8yujp 23 37 and and CC cord-291611-cfe8yujp 23 38 the the DT cord-291611-cfe8yujp 23 39 C C NNP cord-291611-cfe8yujp 23 40 - - HYPH cord-291611-cfe8yujp 23 41 terminal terminal NN cord-291611-cfe8yujp 23 42 half half NN cord-291611-cfe8yujp 23 43 ( ( -LRB- cord-291611-cfe8yujp 23 44 S2 S2 NNP cord-291611-cfe8yujp 23 45 ) ) -RRB- cord-291611-cfe8yujp 23 46 with with IN cord-291611-cfe8yujp 23 47 an an DT cord-291611-cfe8yujp 23 48 approximate approximate JJ cord-291611-cfe8yujp 23 49 mol mol NN cord-291611-cfe8yujp 23 50 . . . cord-291611-cfe8yujp 24 1 wt wt NN cord-291611-cfe8yujp 24 2 . . NNP cord-291611-cfe8yujp 24 3 of of IN cord-291611-cfe8yujp 24 4 100 100 CD cord-291611-cfe8yujp 24 5 kDa kDa NNPS cord-291611-cfe8yujp 24 6 ( ( -LRB- cord-291611-cfe8yujp 24 7 3 3 CD cord-291611-cfe8yujp 24 8 , , , cord-291611-cfe8yujp 24 9 5 5 CD cord-291611-cfe8yujp 24 10 ) ) -RRB- cord-291611-cfe8yujp 24 11 . . . cord-291611-cfe8yujp 25 1 The the DT cord-291611-cfe8yujp 25 2 S S NNP cord-291611-cfe8yujp 25 3 glycoprotein glycoprotein NN cord-291611-cfe8yujp 25 4 functions function NNS cord-291611-cfe8yujp 25 5 in in IN cord-291611-cfe8yujp 25 6 virus virus NN cord-291611-cfe8yujp 25 7 attachment attachment NN cord-291611-cfe8yujp 25 8 to to IN cord-291611-cfe8yujp 25 9 permissive permissive JJ cord-291611-cfe8yujp 25 10 cells cell NNS cord-291611-cfe8yujp 25 11 , , , cord-291611-cfe8yujp 25 12 virus virus NN cord-291611-cfe8yujp 25 13 - - HYPH cord-291611-cfe8yujp 25 14 induced induce VBN cord-291611-cfe8yujp 25 15 cell cell NN cord-291611-cfe8yujp 25 16 fusion fusion NN cord-291611-cfe8yujp 25 17 , , , cord-291611-cfe8yujp 25 18 elicitation elicitation NN cord-291611-cfe8yujp 25 19 of of IN cord-291611-cfe8yujp 25 20 neutralizing neutralize VBG cord-291611-cfe8yujp 25 21 antibodies antibody NNS cord-291611-cfe8yujp 25 22 and and CC cord-291611-cfe8yujp 25 23 cell cell NN cord-291611-cfe8yujp 25 24 - - HYPH cord-291611-cfe8yujp 25 25 mediated mediate VBN cord-291611-cfe8yujp 25 26 immunity immunity NN cord-291611-cfe8yujp 25 27 ( ( -LRB- cord-291611-cfe8yujp 25 28 6 6 CD cord-291611-cfe8yujp 25 29 ) ) -RRB- cord-291611-cfe8yujp 25 30 . . . cord-291611-cfe8yujp 26 1 Two two CD cord-291611-cfe8yujp 26 2 antigenic antigenic JJ cord-291611-cfe8yujp 26 3 domains domain NNS cord-291611-cfe8yujp 26 4 responsible responsible JJ cord-291611-cfe8yujp 26 5 for for IN cord-291611-cfe8yujp 26 6 neutralization neutralization NN cord-291611-cfe8yujp 26 7 of of IN cord-291611-cfe8yujp 26 8 BCV BCV NNP cord-291611-cfe8yujp 26 9 - - HYPH cord-291611-cfe8yujp 26 10 Quebec Quebec NNP cord-291611-cfe8yujp 26 11 and and CC cord-291611-cfe8yujp 26 12 BCV BCV NNP cord-291611-cfe8yujp 26 13 - - HYPH cord-291611-cfe8yujp 26 14 L9 L9 NNP cord-291611-cfe8yujp 26 15 have have VBP cord-291611-cfe8yujp 26 16 been be VBN cord-291611-cfe8yujp 26 17 identified identify VBN cord-291611-cfe8yujp 26 18 on on IN cord-291611-cfe8yujp 26 19 the the DT cord-291611-cfe8yujp 26 20 S S NNP cord-291611-cfe8yujp 26 21 protein protein NN cord-291611-cfe8yujp 26 22 ( ( -LRB- cord-291611-cfe8yujp 26 23 Ref ref NN cord-291611-cfe8yujp 26 24 . . . cord-291611-cfe8yujp 27 1 ( ( -LRB- cord-291611-cfe8yujp 27 2 7 7 CD cord-291611-cfe8yujp 27 3 ) ) -RRB- cord-291611-cfe8yujp 27 4 ; ; : cord-291611-cfe8yujp 27 5 Hussain Hussain NNP cord-291611-cfe8yujp 27 6 et et NNP cord-291611-cfe8yujp 27 7 a/. a/. NNP cord-291611-cfe8yujp 27 8 , , , cord-291611-cfe8yujp 27 9 submitted submit VBD cord-291611-cfe8yujp 27 10 ) ) -RRB- cord-291611-cfe8yujp 27 11 . . . cord-291611-cfe8yujp 28 1 However however RB cord-291611-cfe8yujp 28 2 , , , cord-291611-cfe8yujp 28 3 the the DT cord-291611-cfe8yujp 28 4 location location NN cord-291611-cfe8yujp 28 5 of of IN cord-291611-cfe8yujp 28 6 the the DT cord-291611-cfe8yujp 28 7 neutralizing neutralize VBG cord-291611-cfe8yujp 28 8 epitopes epitope NNS cord-291611-cfe8yujp 28 9 on on IN cord-291611-cfe8yujp 28 10 the the DT cord-291611-cfe8yujp 28 11 BCV BCV NNP cord-291611-cfe8yujp 28 12 S S NNP cord-291611-cfe8yujp 28 13 protein protein NN cord-291611-cfe8yujp 28 14 has have VBZ cord-291611-cfe8yujp 28 15 not not RB cord-291611-cfe8yujp 28 16 yet yet RB cord-291611-cfe8yujp 28 17 been be VBN cord-291611-cfe8yujp 28 18 determined determine VBN cord-291611-cfe8yujp 28 19 . . . cord-291611-cfe8yujp 29 1 Comparison comparison NN cord-291611-cfe8yujp 29 2 of of IN cord-291611-cfe8yujp 29 3 the the DT cord-291611-cfe8yujp 29 4 S s NN cord-291611-cfe8yujp 29 5 proteins protein NNS cord-291611-cfe8yujp 29 6 with with IN cord-291611-cfe8yujp 29 7 S s NN cord-291611-cfe8yujp 29 8 - - HYPH cord-291611-cfe8yujp 29 9 spe- spe- XX cord-291611-cfe8yujp 29 10 ' ' '' cord-291611-cfe8yujp 30 1 To to TO cord-291611-cfe8yujp 30 2 whom whom WP cord-291611-cfe8yujp 30 3 reprint reprint VB cord-291611-cfe8yujp 30 4 requests request NNS cord-291611-cfe8yujp 30 5 should should MD cord-291611-cfe8yujp 30 6 be be VB cord-291611-cfe8yujp 30 7 addressed address VBN cord-291611-cfe8yujp 30 8 cific cific JJ cord-291611-cfe8yujp 30 9 monoclonal monoclonal JJ cord-291611-cfe8yujp 30 10 antibodies antibody NNS cord-291611-cfe8yujp 30 11 ( ( -LRB- cord-291611-cfe8yujp 30 12 MAbs MAbs NNP cord-291611-cfe8yujp 30 13 ) ) -RRB- cord-291611-cfe8yujp 30 14 revealed reveal VBD cord-291611-cfe8yujp 30 15 that that IN cord-291611-cfe8yujp 30 16 different different JJ cord-291611-cfe8yujp 30 17 neutralizing neutralize VBG cord-291611-cfe8yujp 30 18 and and CC cord-291611-cfe8yujp 30 19 non non JJ cord-291611-cfe8yujp 30 20 - - JJ cord-291611-cfe8yujp 30 21 neutralizing neutralizing JJ cord-291611-cfe8yujp 30 22 epitopes epitope NNS cord-291611-cfe8yujp 30 23 as as RB cord-291611-cfe8yujp 30 24 well well RB cord-291611-cfe8yujp 30 25 as as IN cord-291611-cfe8yujp 30 26 conformational conformational JJ cord-291611-cfe8yujp 30 27 epitopes epitope NNS cord-291611-cfe8yujp 30 28 were be VBD cord-291611-cfe8yujp 30 29 present present JJ cord-291611-cfe8yujp 30 30 in in IN cord-291611-cfe8yujp 30 31 the the DT cord-291611-cfe8yujp 30 32 S S NNP cord-291611-cfe8yujp 30 33 glycoproteins glycoprotein NNS cord-291611-cfe8yujp 30 34 specified specify VBN cord-291611-cfe8yujp 30 35 by by IN cord-291611-cfe8yujp 30 36 the the DT cord-291611-cfe8yujp 30 37 virulent virulent JJ cord-291611-cfe8yujp 30 38 and and CC cord-291611-cfe8yujp 30 39 avirulent avirulent JJ cord-291611-cfe8yujp 30 40 strains strain NNS cord-291611-cfe8yujp 30 41 ( ( -LRB- cord-291611-cfe8yujp 30 42 Hussain Hussain NNP cord-291611-cfe8yujp 30 43 et et NNP cord-291611-cfe8yujp 30 44 al al NNP cord-291611-cfe8yujp 30 45 . . NNP cord-291611-cfe8yujp 30 46 , , , cord-291611-cfe8yujp 30 47 submitted submit VBN cord-291611-cfe8yujp 30 48 ) ) -RRB- cord-291611-cfe8yujp 30 49 . . . cord-291611-cfe8yujp 31 1 In in IN cord-291611-cfe8yujp 31 2 order order NN cord-291611-cfe8yujp 31 3 to to TO cord-291611-cfe8yujp 31 4 understand understand VB cord-291611-cfe8yujp 31 5 the the DT cord-291611-cfe8yujp 31 6 molecular molecular JJ cord-291611-cfe8yujp 31 7 basis basis NN cord-291611-cfe8yujp 31 8 of of IN cord-291611-cfe8yujp 31 9 the the DT cord-291611-cfe8yujp 31 10 observed observed JJ cord-291611-cfe8yujp 31 11 antigenic antigenic JJ cord-291611-cfe8yujp 31 12 diversity diversity NN cord-291611-cfe8yujp 31 13 of of IN cord-291611-cfe8yujp 31 14 the the DT cord-291611-cfe8yujp 31 15 S S NNP cord-291611-cfe8yujp 31 16 glycoproteins glycoprotein NNS cord-291611-cfe8yujp 31 17 we -PRON- PRP cord-291611-cfe8yujp 31 18 successfully successfully RB cord-291611-cfe8yujp 31 19 amplified amplify VBD cord-291611-cfe8yujp 31 20 , , , cord-291611-cfe8yujp 31 21 directly directly RB cord-291611-cfe8yujp 31 22 sequenced sequence VBN cord-291611-cfe8yujp 31 23 , , , cord-291611-cfe8yujp 31 24 and and CC cord-291611-cfe8yujp 31 25 cloned clone VBD cord-291611-cfe8yujp 31 26 the the DT cord-291611-cfe8yujp 31 27 entire entire JJ cord-291611-cfe8yujp 31 28 S S NNP cord-291611-cfe8yujp 31 29 genes gene NNS cord-291611-cfe8yujp 31 30 of of IN cord-291611-cfe8yujp 31 31 different different JJ cord-291611-cfe8yujp 31 32 BCV BCV NNP cord-291611-cfe8yujp 31 33 strains strain NNS cord-291611-cfe8yujp 31 34 using use VBG cord-291611-cfe8yujp 31 35 the the DT cord-291611-cfe8yujp 31 36 polymerase polymerase NN cord-291611-cfe8yujp 31 37 chain chain NN cord-291611-cfe8yujp 31 38 reaction reaction NN cord-291611-cfe8yujp 31 39 ( ( -LRB- cord-291611-cfe8yujp 31 40 PCR PCR NNP cord-291611-cfe8yujp 31 41 ) ) -RRB- cord-291611-cfe8yujp 31 42 . . . cord-291611-cfe8yujp 32 1 We -PRON- PRP cord-291611-cfe8yujp 32 2 report report VBP cord-291611-cfe8yujp 32 3 here here RB cord-291611-cfe8yujp 32 4 the the DT cord-291611-cfe8yujp 32 5 sequence sequence NN cord-291611-cfe8yujp 32 6 characteristics characteristic NNS cord-291611-cfe8yujp 32 7 of of IN cord-291611-cfe8yujp 32 8 one one CD cord-291611-cfe8yujp 32 9 virulent virulent NN cord-291611-cfe8yujp 32 10 and and CC cord-291611-cfe8yujp 32 11 two two CD cord-291611-cfe8yujp 32 12 avirulent avirulent NN cord-291611-cfe8yujp 32 13 BCV BCV NNP cord-291611-cfe8yujp 32 14 strains strain NNS cord-291611-cfe8yujp 32 15 and and CC cord-291611-cfe8yujp 32 16 their -PRON- PRP$ cord-291611-cfe8yujp 32 17 comparison comparison NN cord-291611-cfe8yujp 32 18 with with IN cord-291611-cfe8yujp 32 19 the the DT cord-291611-cfe8yujp 32 20 recently recently RB cord-291611-cfe8yujp 32 21 published publish VBN cord-291611-cfe8yujp 32 22 sequences sequence NNS cord-291611-cfe8yujp 32 23 of of IN cord-291611-cfe8yujp 32 24 three three CD cord-291611-cfe8yujp 32 25 other other JJ cord-291611-cfe8yujp 32 26 BCV BCV NNP cord-291611-cfe8yujp 32 27 strains strain NNS cord-291611-cfe8yujp 32 28 . . . cord-291611-cfe8yujp 33 1 The the DT cord-291611-cfe8yujp 33 2 strain strain NN cord-291611-cfe8yujp 33 3 BCV BCV NNP cord-291611-cfe8yujp 33 4 - - HYPH cord-291611-cfe8yujp 33 5 L9 L9 NNP cord-291611-cfe8yujp 33 6 was be VBD cord-291611-cfe8yujp 33 7 derived derive VBN cord-291611-cfe8yujp 33 8 from from IN cord-291611-cfe8yujp 33 9 BCV BCV NNP cord-291611-cfe8yujp 33 10 - - HYPH cord-291611-cfe8yujp 33 11 Mebus Mebus NNP cord-291611-cfe8yujp 33 12 and and CC cord-291611-cfe8yujp 33 13 passaged passage VBD cord-291611-cfe8yujp 33 14 through through IN cord-291611-cfe8yujp 33 15 different different JJ cord-291611-cfe8yujp 33 16 nonpolarized nonpolarize VBN cord-291611-cfe8yujp 33 17 and and CC cord-291611-cfe8yujp 33 18 highly highly RB cord-291611-cfe8yujp 33 19 polarized polarized JJ cord-291611-cfe8yujp 33 20 cells cell NNS cord-291611-cfe8yujp 33 21 over over IN cord-291611-cfe8yujp 33 22 80 80 CD cord-291611-cfe8yujp 33 23 passages passage NNS cord-291611-cfe8yujp 33 24 ( ( -LRB- cord-291611-cfe8yujp 33 25 8) 8) NNP cord-291611-cfe8yujp 33 26 . . . cord-291611-cfe8yujp 34 1 The the DT cord-291611-cfe8yujp 34 2 Norden Norden NNP cord-291611-cfe8yujp 34 3 Vaccine Vaccine NNP cord-291611-cfe8yujp 34 4 strain strain NN cord-291611-cfe8yujp 34 5 ( ( -LRB- cord-291611-cfe8yujp 34 6 BCV BCV NNP cord-291611-cfe8yujp 34 7 - - HYPH cord-291611-cfe8yujp 34 8 Vaccine Vaccine NNP cord-291611-cfe8yujp 34 9 ) ) -RRB- cord-291611-cfe8yujp 34 10 was be VBD cord-291611-cfe8yujp 34 11 also also RB cord-291611-cfe8yujp 34 12 derived derive VBN cord-291611-cfe8yujp 34 13 from from IN cord-291611-cfe8yujp 34 14 BCV BCV NNP cord-291611-cfe8yujp 34 15 - - HYPH cord-291611-cfe8yujp 34 16 Mebus Mebus NNP cord-291611-cfe8yujp 34 17 and and CC cord-291611-cfe8yujp 34 18 used use VBD cord-291611-cfe8yujp 34 19 asvaccine asvaccine NN cord-291611-cfe8yujp 34 20 by by IN cord-291611-cfe8yujp 34 21 Norden Norden NNP cord-291611-cfe8yujp 34 22 Laboratories Laboratories NNP cord-291611-cfe8yujp 34 23 ( ( -LRB- cord-291611-cfe8yujp 34 24 Omaha Omaha NNP cord-291611-cfe8yujp 34 25 , , , cord-291611-cfe8yujp 34 26 NE NE NNP cord-291611-cfe8yujp 34 27 ) ) -RRB- cord-291611-cfe8yujp 34 28 ( ( -LRB- cord-291611-cfe8yujp 34 29 1 1 CD cord-291611-cfe8yujp 34 30 , , , cord-291611-cfe8yujp 34 31 9 9 CD cord-291611-cfe8yujp 34 32 ) ) -RRB- cord-291611-cfe8yujp 34 33 . . . cord-291611-cfe8yujp 35 1 The the DT cord-291611-cfe8yujp 35 2 highly highly RB cord-291611-cfe8yujp 35 3 virulent virulent JJ cord-291611-cfe8yujp 35 4 wildtype wildtype NN cord-291611-cfe8yujp 35 5 strain strain NN cord-291611-cfe8yujp 35 6 BCV BCV NNP cord-291611-cfe8yujp 35 7 - - HYPH cord-291611-cfe8yujp 35 8 LY LY NNP cord-291611-cfe8yujp 35 9 138 138 CD cord-291611-cfe8yujp 35 10 was be VBD cord-291611-cfe8yujp 35 11 isolated isolate VBN cord-291611-cfe8yujp 35 12 from from IN cord-291611-cfe8yujp 35 13 diarrhea1 diarrhea1 JJ cord-291611-cfe8yujp 35 14 fluid fluid NN cord-291611-cfe8yujp 35 15 of of IN cord-291611-cfe8yujp 35 16 a a DT cord-291611-cfe8yujp 35 17 diseased diseased JJ cord-291611-cfe8yujp 35 18 calf calf NN cord-291611-cfe8yujp 35 19 in in IN cord-291611-cfe8yujp 35 20 1965 1965 CD cord-291611-cfe8yujp 35 21 in in IN cord-291611-cfe8yujp 35 22 Utah Utah NNP cord-291611-cfe8yujp 35 23 / / SYM cord-291611-cfe8yujp 35 24 USA USA NNP cord-291611-cfe8yujp 35 25 ( ( -LRB- cord-291611-cfe8yujp 35 26 IO IO NNP cord-291611-cfe8yujp 35 27 ) ) -RRB- cord-291611-cfe8yujp 35 28 , , , cord-291611-cfe8yujp 35 29 and and CC cord-291611-cfe8yujp 35 30 maintained maintain VBD cord-291611-cfe8yujp 35 31 in in IN cord-291611-cfe8yujp 35 32 calves calf NNS cord-291611-cfe8yujp 35 33 through through IN cord-291611-cfe8yujp 35 34 oral oral JJ cord-291611-cfe8yujp 35 35 inoculation inoculation NN cord-291611-cfe8yujp 35 36 since since IN cord-291611-cfe8yujp 35 37 then then RB cord-291611-cfe8yujp 35 38 ( ( -LRB- cord-291611-cfe8yujp 35 39 8 8 CD cord-291611-cfe8yujp 35 40 , , , cord-291611-cfe8yujp 35 41 11 11 CD cord-291611-cfe8yujp 35 42 ) ) -RRB- cord-291611-cfe8yujp 35 43 . . . cord-291611-cfe8yujp 36 1 This this DT cord-291611-cfe8yujp 36 2 strain strain NN cord-291611-cfe8yujp 36 3 replicates replicate VBZ cord-291611-cfe8yujp 36 4 only only RB cord-291611-cfe8yujp 36 5 in in IN cord-291611-cfe8yujp 36 6 HRT-18 HRT-18 NNP cord-291611-cfe8yujp 36 7 cells cell NNS cord-291611-cfe8yujp 36 8 but but CC cord-291611-cfe8yujp 36 9 not not RB cord-291611-cfe8yujp 36 10 in in IN cord-291611-cfe8yujp 36 11 numerous numerous JJ cord-291611-cfe8yujp 36 12 bovine bovine JJ cord-291611-cfe8yujp 36 13 cells cell NNS cord-291611-cfe8yujp 36 14 ( ( -LRB- cord-291611-cfe8yujp 36 15 8) 8) NNP cord-291611-cfe8yujp 36 16 . . . cord-291611-cfe8yujp 37 1 All all DT cord-291611-cfe8yujp 37 2 strains strain NNS cord-291611-cfe8yujp 37 3 were be VBD cord-291611-cfe8yujp 37 4 propagated propagate VBN cord-291611-cfe8yujp 37 5 in in IN cord-291611-cfe8yujp 37 6 HRT-18 HRT-18 NNP cord-291611-cfe8yujp 37 7 cells cell NNS cord-291611-cfe8yujp 37 8 as as IN cord-291611-cfe8yujp 37 9 described describe VBN cord-291611-cfe8yujp 37 10 previously previously RB cord-291611-cfe8yujp 37 11 ( ( -LRB- cord-291611-cfe8yujp 37 12 8) 8) NNP cord-291611-cfe8yujp 37 13 . . . cord-291611-cfe8yujp 38 1 After after IN cord-291611-cfe8yujp 38 2 24 24 CD cord-291611-cfe8yujp 38 3 h.p.i h.p.i NNP cord-291611-cfe8yujp 38 4 . . NNP cord-291611-cfe8yujp 38 5 , , , cord-291611-cfe8yujp 38 6 cells cell NNS cord-291611-cfe8yujp 38 7 were be VBD cord-291611-cfe8yujp 38 8 washed wash VBN cord-291611-cfe8yujp 38 9 twice twice RB cord-291611-cfe8yujp 38 10 with with IN cord-291611-cfe8yujp 38 11 phos phos NNP cord-291611-cfe8yujp 38 12 - - HYPH cord-291611-cfe8yujp 38 13 phate phate NNP cord-291611-cfe8yujp 38 14 buffered buffer VBN cord-291611-cfe8yujp 38 15 saline saline NN cord-291611-cfe8yujp 38 16 ( ( -LRB- cord-291611-cfe8yujp 38 17 PBS PBS NNP cord-291611-cfe8yujp 38 18 ) ) -RRB- cord-291611-cfe8yujp 38 19 , , , cord-291611-cfe8yujp 38 20 and and CC cord-291611-cfe8yujp 38 21 RNA RNA NNP cord-291611-cfe8yujp 38 22 was be VBD cord-291611-cfe8yujp 38 23 isolated isolate VBN cord-291611-cfe8yujp 38 24 using use VBG cord-291611-cfe8yujp 38 25 isothiocyanate isothiocyanate NN cord-291611-cfe8yujp 38 26 / / SYM cord-291611-cfe8yujp 38 27 cesium cesium NN cord-291611-cfe8yujp 38 28 chloride chloride NN cord-291611-cfe8yujp 38 29 gradients gradient NNS cord-291611-cfe8yujp 38 30 according accord VBG cord-291611-cfe8yujp 38 31 to to IN cord-291611-cfe8yujp 38 32 the the DT cord-291611-cfe8yujp 38 33 method method NN cord-291611-cfe8yujp 38 34 described describe VBN cord-291611-cfe8yujp 38 35 previously previously RB cord-291611-cfe8yujp 38 36 ( ( -LRB- cord-291611-cfe8yujp 38 37 12 12 CD cord-291611-cfe8yujp 38 38 ) ) -RRB- cord-291611-cfe8yujp 38 39 . . . cord-291611-cfe8yujp 39 1 As as IN cord-291611-cfe8yujp 39 2 control control NN cord-291611-cfe8yujp 39 3 , , , cord-291611-cfe8yujp 39 4 RNA RNA NNP cord-291611-cfe8yujp 39 5 was be VBD cord-291611-cfe8yujp 39 6 isolated isolate VBN cord-291611-cfe8yujp 39 7 from from IN cord-291611-cfe8yujp 39 8 uninfected uninfected JJ cord-291611-cfe8yujp 39 9 cells cell NNS cord-291611-cfe8yujp 39 10 . . . cord-291611-cfe8yujp 40 1 The the DT cord-291611-cfe8yujp 40 2 first first JJ cord-291611-cfe8yujp 40 3 - - HYPH cord-291611-cfe8yujp 40 4 strand strand NN cord-291611-cfe8yujp 40 5 cDNA cdna NN cord-291611-cfe8yujp 40 6 synthesis synthesis NN cord-291611-cfe8yujp 40 7 was be VBD cord-291611-cfe8yujp 40 8 carried carry VBN cord-291611-cfe8yujp 40 9 out out RP cord-291611-cfe8yujp 40 10 in in IN cord-291611-cfe8yujp 40 11 a a DT cord-291611-cfe8yujp 40 12 volume volume NN cord-291611-cfe8yujp 40 13 of of IN cord-291611-cfe8yujp 40 14 25 25 CD cord-291611-cfe8yujp 40 15 ~1 ~1 DT cord-291611-cfe8yujp 40 16 containing contain VBG cord-291611-cfe8yujp 40 17 50 50 CD cord-291611-cfe8yujp 40 18 mh mh NNP cord-291611-cfe8yujp 40 19 / / SYM cord-291611-cfe8yujp 40 20 l l NNP cord-291611-cfe8yujp 40 21 Tris Tris NNP cord-291611-cfe8yujp 40 22 - - HYPH cord-291611-cfe8yujp 40 23 HCI HCI NNP cord-291611-cfe8yujp 40 24 ( ( -LRB- cord-291611-cfe8yujp 40 25 pH pH NNP cord-291611-cfe8yujp 40 26 8.3 8.3 CD cord-291611-cfe8yujp 40 27 ) ) -RRB- cord-291611-cfe8yujp 40 28 , , , cord-291611-cfe8yujp 40 29 50mILIKCI,3mMMgCI 50milikci,3mmmgci CD cord-291611-cfe8yujp 40 30 , , , cord-291611-cfe8yujp 40 31 , , , cord-291611-cfe8yujp 40 32 lOmMDlT lOmMDlT NNP cord-291611-cfe8yujp 40 33 , , , cord-291611-cfe8yujp 40 34 1 1 CD cord-291611-cfe8yujp 40 35 mMeachof mMeachof NNP cord-291611-cfe8yujp 40 36 the the DT cord-291611-cfe8yujp 40 37 four four CD cord-291611-cfe8yujp 40 38 dNTPs dntp NNS cord-291611-cfe8yujp 40 39 , , . cord-291611-cfe8yujp 41 1 25 25 CD cord-291611-cfe8yujp 41 2 U u NN cord-291611-cfe8yujp 41 3 RNAsin rnasin NN cord-291611-cfe8yujp 42 1 ( ( -LRB- cord-291611-cfe8yujp 42 2 Bethesda Bethesda NNP cord-291611-cfe8yujp 42 3 Research Research NNP cord-291611-cfe8yujp 42 4 Laboratories Laboratories NNP cord-291611-cfe8yujp 42 5 ( ( -LRB- cord-291611-cfe8yujp 42 6 BRL BRL NNP cord-291611-cfe8yujp 42 7 ) ) -RRB- cord-291611-cfe8yujp 42 8 ) ) -RRB- cord-291611-cfe8yujp 42 9 , , . cord-291611-cfe8yujp 43 1 2'pM 2'pm CD cord-291611-cfe8yujp 43 2 3 3 CD cord-291611-cfe8yujp 43 3 ' ' NN cord-291611-cfe8yujp 43 4 primer primer NN cord-291611-cfe8yujp 43 5 , , , cord-291611-cfe8yujp 43 6 1 1 CD cord-291611-cfe8yujp 43 7 pg pg NNP cord-291611-cfe8yujp 43 8 total total NN cord-291611-cfe8yujp 43 9 RNA RNA NNP cord-291611-cfe8yujp 43 10 , , , cord-291611-cfe8yujp 43 11 200 200 CD cord-291611-cfe8yujp 43 12 U U NNP cord-291611-cfe8yujp 43 13 Moloney Moloney NNP cord-291611-cfe8yujp 43 14 murine murine JJ cord-291611-cfe8yujp 43 15 leukemia leukemia NN cord-291611-cfe8yujp 43 16 virus virus NN cord-291611-cfe8yujp 43 17 reverse reverse NN cord-291611-cfe8yujp 43 18 transcriptase transcriptase NN cord-291611-cfe8yujp 43 19 ( ( -LRB- cord-291611-cfe8yujp 43 20 M M NNP cord-291611-cfe8yujp 43 21 - - HYPH cord-291611-cfe8yujp 43 22 MLV MLV NNP cord-291611-cfe8yujp 43 23 RT RT NNP cord-291611-cfe8yujp 43 24 ) ) -RRB- cord-291611-cfe8yujp 44 1 ( ( -LRB- cord-291611-cfe8yujp 44 2 BRL BRL NNP cord-291611-cfe8yujp 44 3 ) ) -RRB- cord-291611-cfe8yujp 44 4 for for IN cord-291611-cfe8yujp 44 5 1 1 CD cord-291611-cfe8yujp 44 6 hr hr NN cord-291611-cfe8yujp 44 7 at at IN cord-291611-cfe8yujp 44 8 42 42 CD cord-291611-cfe8yujp 44 9 " " NNS cord-291611-cfe8yujp 44 10 , , , cord-291611-cfe8yujp 44 11 and and CC cord-291611-cfe8yujp 44 12 the the DT cord-291611-cfe8yujp 44 13 reaction reaction NN cord-291611-cfe8yujp 44 14 was be VBD cord-291611-cfe8yujp 44 15 stopped stop VBN cord-291611-cfe8yujp 44 16 by by IN cord-291611-cfe8yujp 44 17 heating heat VBG cord-291611-cfe8yujp 44 18 samples sample NNS cord-291611-cfe8yujp 44 19 to to IN cord-291611-cfe8yujp 44 20 95 95 CD cord-291611-cfe8yujp 44 21 " " '' cord-291611-cfe8yujp 44 22 for for IN cord-291611-cfe8yujp 44 23 10 10 CD cord-291611-cfe8yujp 44 24 min min NN cord-291611-cfe8yujp 44 25 and and CC cord-291611-cfe8yujp 44 26 then then RB cord-291611-cfe8yujp 44 27 cooled cool VBD cord-291611-cfe8yujp 44 28 on on IN cord-291611-cfe8yujp 44 29 ice ice NN cord-291611-cfe8yujp 44 30 . . . cord-291611-cfe8yujp 45 1 The the DT cord-291611-cfe8yujp 45 2 RNA RNA NNP cord-291611-cfe8yujp 45 3 was be VBD cord-291611-cfe8yujp 45 4 denatured denature VBN cord-291611-cfe8yujp 45 5 after after IN cord-291611-cfe8yujp 45 6 adding add VBG cord-291611-cfe8yujp 45 7 20 20 CD cord-291611-cfe8yujp 45 8 m m CD cord-291611-cfe8yujp 45 9 11/1 11/1 CD cord-291611-cfe8yujp 45 10 methylmercuric methylmercuric NNP cord-291611-cfe8yujp 45 11 hydroxide hydroxide NNP cord-291611-cfe8yujp 45 12 ( ( -LRB- cord-291611-cfe8yujp 45 13 MeHgOH MeHgOH NNP cord-291611-cfe8yujp 45 14 ) ) -RRB- cord-291611-cfe8yujp 45 15 and and CC cord-291611-cfe8yujp 45 16 70 70 CD cord-291611-cfe8yujp 45 17 m m NN cord-291611-cfe8yujp 45 18 M M NNP cord-291611-cfe8yujp 45 19 2-mercaptoethanol 2-mercaptoethanol CD cord-291611-cfe8yujp 45 20 for for IN cord-291611-cfe8yujp 45 21 7 7 CD cord-291611-cfe8yujp 45 22 and and CC cord-291611-cfe8yujp 45 23 5 5 CD cord-291611-cfe8yujp 45 24 min min NN cord-291611-cfe8yujp 45 25 at at IN cord-291611-cfe8yujp 45 26 room room NN cord-291611-cfe8yujp 45 27 temperature temperature NN cord-291611-cfe8yujp 45 28 , , , cord-291611-cfe8yujp 45 29 respectively respectively RB cord-291611-cfe8yujp 45 30 , , , cord-291611-cfe8yujp 45 31 incubated incubate VBN cord-291611-cfe8yujp 45 32 with with IN cord-291611-cfe8yujp 45 33 oligonucleotide oligonucleotide JJ cord-291611-cfe8yujp 45 34 primer primer NN cord-291611-cfe8yujp 45 35 at at IN cord-291611-cfe8yujp 45 36 65 65 CD cord-291611-cfe8yujp 45 37 " " `` cord-291611-cfe8yujp 45 38 for for IN cord-291611-cfe8yujp 45 39 2 2 CD cord-291611-cfe8yujp 45 40 min min NN cord-291611-cfe8yujp 45 41 , , , cord-291611-cfe8yujp 45 42 and and CC cord-291611-cfe8yujp 45 43 then then RB cord-291611-cfe8yujp 45 44 chilled chill VBD cord-291611-cfe8yujp 45 45 on on IN cord-291611-cfe8yujp 45 46 ice ice NN cord-291611-cfe8yujp 45 47 before before IN cord-291611-cfe8yujp 45 48 adding add VBG cord-291611-cfe8yujp 45 49 other other JJ cord-291611-cfe8yujp 45 50 reagents reagent NNS cord-291611-cfe8yujp 45 51 . . . cord-291611-cfe8yujp 46 1 Two two CD cord-291611-cfe8yujp 46 2 S s NN cord-291611-cfe8yujp 46 3 gene gene NN cord-291611-cfe8yujp 46 4 specific specific JJ cord-291611-cfe8yujp 46 5 primers primer NNS cord-291611-cfe8yujp 46 6 were be VBD cord-291611-cfe8yujp 46 7 designed design VBN cord-291611-cfe8yujp 46 8 both both DT cord-291611-cfe8yujp 46 9 for for IN cord-291611-cfe8yujp 46 10 cDNA cdna NN cord-291611-cfe8yujp 46 11 synthesis synthesis NN cord-291611-cfe8yujp 46 12 and and CC cord-291611-cfe8yujp 46 13 PCR PCR NNP cord-291611-cfe8yujp 46 14 amplification amplification NN cord-291611-cfe8yujp 46 15 according accord VBG cord-291611-cfe8yujp 46 16 to to IN cord-291611-cfe8yujp 46 17 the the DT cord-291611-cfe8yujp 46 18 published publish VBN cord-291611-cfe8yujp 46 19 sequence sequence NN cord-291611-cfe8yujp 46 20 ( ( -LRB- cord-291611-cfe8yujp 46 21 13 13 CD cord-291611-cfe8yujp 46 22 ) ) -RRB- cord-291611-cfe8yujp 46 23 . . . cord-291611-cfe8yujp 47 1 The the DT cord-291611-cfe8yujp 47 2 A3 A3 NNP cord-291611-cfe8yujp 47 3 ' ' POS cord-291611-cfe8yujp 47 4 primer primer NN cord-291611-cfe8yujp 47 5 is be VBZ cord-291611-cfe8yujp 47 6 antisense antisense NN cord-291611-cfe8yujp 47 7 representing represent VBG cord-291611-cfe8yujp 47 8 the the DT cord-291611-cfe8yujp 47 9 sequence sequence NN cord-291611-cfe8yujp 47 10 at at IN cord-291611-cfe8yujp 47 11 the the DT cord-291611-cfe8yujp 47 12 positions position NNS cord-291611-cfe8yujp 47 13 2 2 CD cord-291611-cfe8yujp 47 14 165 165 CD cord-291611-cfe8yujp 47 15 - - SYM cord-291611-cfe8yujp 47 16 2 2 CD cord-291611-cfe8yujp 47 17 187 187 CD cord-291611-cfe8yujp 47 18 ( ( -LRB- cord-291611-cfe8yujp 47 19 5'-7TTGGATCCAGG 5'-7ttggatccagg CD cord-291611-cfe8yujp 47 20 - - HYPH cord-291611-cfe8yujp 47 21 TTGCAGCTGTCGTGAAAGA-3 TTGCAGCTGTCGTGAAAGA-3 NNP cord-291611-cfe8yujp 47 22 ' ' POS cord-291611-cfe8yujp 47 23 ) ) -RRB- cord-291611-cfe8yujp 47 24 . . . cord-291611-cfe8yujp 48 1 The the DT cord-291611-cfe8yujp 48 2 B3 b3 NN cord-291611-cfe8yujp 48 3 ' ' '' cord-291611-cfe8yujp 48 4 primer primer NN cord-291611-cfe8yujp 48 5 corresponds correspond VBZ cord-291611-cfe8yujp 48 6 to to IN cord-291611-cfe8yujp 48 7 the the DT cord-291611-cfe8yujp 48 8 sequence sequence NN cord-291611-cfe8yujp 48 9 downstream downstream RB cord-291611-cfe8yujp 48 10 of of IN cord-291611-cfe8yujp 48 11 the the DT cord-291611-cfe8yujp 48 12 S S NNP cord-291611-cfe8yujp 48 13 gene gene NN cord-291611-cfe8yujp 48 14 ( ( -LRB- cord-291611-cfe8yujp 48 15 5'-AACGGATCCAATATATCGTCAGGAGCC 5'-AACGGATCCAATATATCGTCAGGAGCC NNP cord-291611-cfe8yujp 48 16 - - HYPH cord-291611-cfe8yujp 48 17 AATA-3 aata-3 NN cord-291611-cfe8yujp 48 18 ' ' '' cord-291611-cfe8yujp 48 19 ) ) -RRB- cord-291611-cfe8yujp 48 20 . . . cord-291611-cfe8yujp 49 1 Both both DT cord-291611-cfe8yujp 49 2 primers primer NNS cord-291611-cfe8yujp 49 3 contain contain VBP cord-291611-cfe8yujp 49 4 an an DT cord-291611-cfe8yujp 49 5 extra extra JJ cord-291611-cfe8yujp 49 6 BarnHI BarnHI NNP cord-291611-cfe8yujp 49 7 linker linker NN cord-291611-cfe8yujp 49 8 in in IN cord-291611-cfe8yujp 49 9 the the DT cord-291611-cfe8yujp 49 10 5 5 CD cord-291611-cfe8yujp 49 11 ' ' NN cord-291611-cfe8yujp 49 12 end end NN cord-291611-cfe8yujp 49 13 for for IN cord-291611-cfe8yujp 49 14 cloning clone VBG cord-291611-cfe8yujp 49 15 purposes purpose NNS cord-291611-cfe8yujp 49 16 . . . cord-291611-cfe8yujp 50 1 The the DT cord-291611-cfe8yujp 50 2 primers primer NNS cord-291611-cfe8yujp 50 3 were be VBD cord-291611-cfe8yujp 50 4 synthesized synthesize VBN cord-291611-cfe8yujp 50 5 in in IN cord-291611-cfe8yujp 50 6 the the DT cord-291611-cfe8yujp 50 7 Gene Gene NNP cord-291611-cfe8yujp 50 8 Assembler Assembler NNP cord-291611-cfe8yujp 50 9 ( ( -LRB- cord-291611-cfe8yujp 50 10 Pharmacia Pharmacia NNP cord-291611-cfe8yujp 50 11 - - HYPH cord-291611-cfe8yujp 50 12 LKB LKB NNP cord-291611-cfe8yujp 50 13 , , , cord-291611-cfe8yujp 50 14 Piscataway Piscataway NNP cord-291611-cfe8yujp 50 15 , , , cord-291611-cfe8yujp 50 16 NJ NJ NNP cord-291611-cfe8yujp 50 17 ) ) -RRB- cord-291611-cfe8yujp 50 18 in in IN cord-291611-cfe8yujp 50 19 our -PRON- PRP$ cord-291611-cfe8yujp 50 20 laboratory laboratory NN cord-291611-cfe8yujp 50 21 according accord VBG cord-291611-cfe8yujp 50 22 to to IN cord-291611-cfe8yujp 50 23 the the DT cord-291611-cfe8yujp 50 24 manufacturer manufacturer NN cord-291611-cfe8yujp 50 25 's 's POS cord-291611-cfe8yujp 50 26 instructions instruction NNS cord-291611-cfe8yujp 50 27 , , , cord-291611-cfe8yujp 50 28 and and CC cord-291611-cfe8yujp 50 29 purified purify VBN cord-291611-cfe8yujp 50 30 using use VBG cord-291611-cfe8yujp 50 31 Poly Poly NNP cord-291611-cfe8yujp 50 32 - - HYPH cord-291611-cfe8yujp 50 33 PAK PAK NNP cord-291611-cfe8yujp 50 34 ( ( -LRB- cord-291611-cfe8yujp 50 35 Glen Glen NNP cord-291611-cfe8yujp 50 36 Research Research NNP cord-291611-cfe8yujp 50 37 , , , cord-291611-cfe8yujp 50 38 Herndon Herndon NNP cord-291611-cfe8yujp 50 39 , , , cord-291611-cfe8yujp 50 40 VA VA NNP cord-291611-cfe8yujp 50 41 ) ) -RRB- cord-291611-cfe8yujp 50 42 or or CC cord-291611-cfe8yujp 50 43 Oligo Oligo NNP cord-291611-cfe8yujp 50 44 - - HYPH cord-291611-cfe8yujp 50 45 PAK PAK NNP cord-291611-cfe8yujp 50 46 column column NN cord-291611-cfe8yujp 50 47 ( ( -LRB- cord-291611-cfe8yujp 50 48 Millipore Millipore NNP cord-291611-cfe8yujp 50 49 , , , cord-291611-cfe8yujp 50 50 Burlington Burlington NNP cord-291611-cfe8yujp 50 51 , , , cord-291611-cfe8yujp 50 52 MA MA NNP cord-291611-cfe8yujp 50 53 ) ) -RRB- cord-291611-cfe8yujp 50 54 . . . cord-291611-cfe8yujp 51 1 The the DT cord-291611-cfe8yujp 51 2 reaction reaction NN cord-291611-cfe8yujp 51 3 of of IN cord-291611-cfe8yujp 51 4 second second JJ cord-291611-cfe8yujp 51 5 strand strand NN cord-291611-cfe8yujp 51 6 cDNA cdna NN cord-291611-cfe8yujp 51 7 synthesis synthesis NN cord-291611-cfe8yujp 51 8 and and CC cord-291611-cfe8yujp 51 9 amplification amplification NN cord-291611-cfe8yujp 51 10 was be VBD cord-291611-cfe8yujp 51 11 carried carry VBN cord-291611-cfe8yujp 51 12 out out RP cord-291611-cfe8yujp 51 13 in in IN cord-291611-cfe8yujp 51 14 PCR PCR NNP cord-291611-cfe8yujp 51 15 ( ( -LRB- cord-291611-cfe8yujp 51 16 MicroCycler MicroCycler NNP cord-291611-cfe8yujp 51 17 , , , cord-291611-cfe8yujp 51 18 Epperidot Epperidot NNP cord-291611-cfe8yujp 51 19 - - HYPH cord-291611-cfe8yujp 51 20 f f NNP cord-291611-cfe8yujp 51 21 Inc. Inc. NNP cord-291611-cfe8yujp 51 22 , , , cord-291611-cfe8yujp 51 23 Fremont Fremont NNP cord-291611-cfe8yujp 51 24 , , , cord-291611-cfe8yujp 51 25 CA CA NNP cord-291611-cfe8yujp 51 26 ) ) -RRB- cord-291611-cfe8yujp 51 27 with with IN cord-291611-cfe8yujp 51 28 5 5 CD cord-291611-cfe8yujp 51 29 ~1 ~1 CD cord-291611-cfe8yujp 51 30 of of IN cord-291611-cfe8yujp 51 31 the the DT cord-291611-cfe8yujp 51 32 reverse reverse JJ cord-291611-cfe8yujp 51 33 transcription transcription NN cord-291611-cfe8yujp 51 34 reaction reaction NN cord-291611-cfe8yujp 51 35 in in IN cord-291611-cfe8yujp 51 36 a a DT cord-291611-cfe8yujp 51 37 total total JJ cord-291611-cfe8yujp 51 38 volume volume NN cord-291611-cfe8yujp 51 39 of of IN cord-291611-cfe8yujp 51 40 100 100 CD cord-291611-cfe8yujp 51 41 ~1 ~1 DT cord-291611-cfe8yujp 51 42 containing contain VBG cord-291611-cfe8yujp 51 43 20mMTris 20mmtris CD cord-291611-cfe8yujp 51 44 - - HYPH cord-291611-cfe8yujp 51 45 HCl(pH8.3),25mMKCI hcl(ph8.3),25mmkci NN cord-291611-cfe8yujp 51 46 , , , cord-291611-cfe8yujp 51 47 1.5mMMgCI 1.5mmmgci CD cord-291611-cfe8yujp 51 48 , , , cord-291611-cfe8yujp 51 49 , , , cord-291611-cfe8yujp 51 50 0.1 0.1 CD cord-291611-cfe8yujp 51 51 % % NN cord-291611-cfe8yujp 51 52 Tween Tween NNP cord-291611-cfe8yujp 51 53 20 20 CD cord-291611-cfe8yujp 51 54 , , , cord-291611-cfe8yujp 51 55 10 10 CD cord-291611-cfe8yujp 51 56 pg pg CD cord-291611-cfe8yujp 51 57 of of IN cord-291611-cfe8yujp 51 58 bovine bovine JJ cord-291611-cfe8yujp 51 59 serum serum NN cord-291611-cfe8yujp 51 60 albumin albumin NN cord-291611-cfe8yujp 51 61 ( ( -LRB- cord-291611-cfe8yujp 51 62 BSA BSA NNP cord-291611-cfe8yujp 51 63 ) ) -RRB- cord-291611-cfe8yujp 51 64 , , , cord-291611-cfe8yujp 51 65 2.5 2.5 CD cord-291611-cfe8yujp 51 66 U u NN cord-291611-cfe8yujp 51 67 of of IN cord-291611-cfe8yujp 51 68 Taq taq NN cord-291611-cfe8yujp 51 69 DNA dna NN cord-291611-cfe8yujp 51 70 polymerase polymerase NN cord-291611-cfe8yujp 51 71 ( ( -LRB- cord-291611-cfe8yujp 51 72 BRL BRL NNP cord-291611-cfe8yujp 51 73 ) ) -RRB- cord-291611-cfe8yujp 51 74 , , , cord-291611-cfe8yujp 51 75 200 200 CD cord-291611-cfe8yujp 51 76 & & CC cord-291611-cfe8yujp 51 77 l l NNP cord-291611-cfe8yujp 51 78 each each DT cord-291611-cfe8yujp 51 79 of of IN cord-291611-cfe8yujp 51 80 the the DT cord-291611-cfe8yujp 51 81 four four CD cord-291611-cfe8yujp 51 82 dNTPs dntps CD cord-291611-cfe8yujp 51 83 , , , cord-291611-cfe8yujp 51 84 and and CC cord-291611-cfe8yujp 51 85 20 20 CD cord-291611-cfe8yujp 51 86 pmol pmol NN cord-291611-cfe8yujp 51 87 each each DT cord-291611-cfe8yujp 51 88 of of IN cord-291611-cfe8yujp 51 89 the the DT cord-291611-cfe8yujp 51 90 primers primer NNS cord-291611-cfe8yujp 51 91 . . . cord-291611-cfe8yujp 52 1 The the DT cord-291611-cfe8yujp 52 2 sense sense NN cord-291611-cfe8yujp 52 3 A5 a5 NN cord-291611-cfe8yujp 52 4 ' ' '' cord-291611-cfe8yujp 52 5 primer primer NN cord-291611-cfe8yujp 52 6 corresponds correspond VBZ cord-291611-cfe8yujp 52 7 to to IN cord-291611-cfe8yujp 52 8 the the DT cord-291611-cfe8yujp 52 9 sequence sequence NN cord-291611-cfe8yujp 52 10 upstream upstream RB cord-291611-cfe8yujp 52 11 of of IN cord-291611-cfe8yujp 52 12 the the DT cord-291611-cfe8yujp 52 13 S S NNP cord-291611-cfe8yujp 52 14 gene gene NN cord-291611-cfe8yujp 52 15 ( ( -LRB- cord-291611-cfe8yujp 52 16 5'-GCTGAAnCGATAATGGT 5'-gctgaancgataatggt CD cord-291611-cfe8yujp 52 17 - - HYPH cord-291611-cfe8yujp 52 18 ACTAGGCTGCATGAT-3 ACTAGGCTGCATGAT-3 NNP cord-291611-cfe8yujp 52 19 ' ' '' cord-291611-cfe8yujp 52 20 ) ) -RRB- cord-291611-cfe8yujp 52 21 , , , cord-291611-cfe8yujp 52 22 and and CC cord-291611-cfe8yujp 52 23 the the DT cord-291611-cfe8yujp 52 24 B5 B5 NNP cord-291611-cfe8yujp 52 25 ' ' POS cord-291611-cfe8yujp 52 26 primer primer NN cord-291611-cfe8yujp 52 27 represents represent VBZ cord-291611-cfe8yujp 52 28 the the DT cord-291611-cfe8yujp 52 29 sequence sequence NN cord-291611-cfe8yujp 52 30 at at IN cord-291611-cfe8yujp 52 31 the the DT cord-291611-cfe8yujp 52 32 positions position NNS cord-291611-cfe8yujp 52 33 2 2 CD cord-291611-cfe8yujp 52 34 165 165 CD cord-291611-cfe8yujp 52 35 - - SYM cord-291611-cfe8yujp 52 36 2 2 CD cord-291611-cfe8yujp 52 37 187 187 CD cord-291611-cfe8yujp 52 38 ( ( -LRB- cord-291611-cfe8yujp 52 39 5'-GCTGAA 5'-gctgaa CD cord-291611-cfe8yujp 52 40 TTCTCTTTCACGACAGCTGCAACCT-3 TTCTCTTTCACGACAGCTGCAACCT-3 NNP cord-291611-cfe8yujp 52 41 ' ' '' cord-291611-cfe8yujp 52 42 ) ) -RRB- cord-291611-cfe8yujp 52 43 . . . cord-291611-cfe8yujp 53 1 These these DT cord-291611-cfe8yujp 53 2 primers primer NNS cord-291611-cfe8yujp 53 3 contain contain VBP cord-291611-cfe8yujp 53 4 an an DT cord-291611-cfe8yujp 53 5 extra extra JJ cord-291611-cfe8yujp 53 6 EcoRl ecorl NN cord-291611-cfe8yujp 53 7 linker linker NN cord-291611-cfe8yujp 53 8 on on IN cord-291611-cfe8yujp 53 9 the the DT cord-291611-cfe8yujp 53 10 5 5 CD cord-291611-cfe8yujp 53 11 ' ' NN cord-291611-cfe8yujp 53 12 end end NN cord-291611-cfe8yujp 53 13 as as IN cord-291611-cfe8yujp 53 14 indicated indicate VBN cord-291611-cfe8yujp 53 15 . . . cord-291611-cfe8yujp 54 1 Each each DT cord-291611-cfe8yujp 54 2 cycle cycle NN cord-291611-cfe8yujp 54 3 consisted consist VBD cord-291611-cfe8yujp 54 4 of of IN cord-291611-cfe8yujp 54 5 a a DT cord-291611-cfe8yujp 54 6 1 1 CD cord-291611-cfe8yujp 54 7 -set -set HYPH cord-291611-cfe8yujp 54 8 denaturation denaturation NN cord-291611-cfe8yujp 54 9 at at IN cord-291611-cfe8yujp 54 10 96 96 CD cord-291611-cfe8yujp 54 11 " " NNS cord-291611-cfe8yujp 54 12 , , , cord-291611-cfe8yujp 54 13 followed follow VBN cord-291611-cfe8yujp 54 14 by by IN cord-291611-cfe8yujp 54 15 30-set 30-set CD cord-291611-cfe8yujp 54 16 annealing anneal VBG cord-291611-cfe8yujp 54 17 ( ( -LRB- cord-291611-cfe8yujp 54 18 at at IN cord-291611-cfe8yujp 54 19 59 59 CD cord-291611-cfe8yujp 54 20 " " '' cord-291611-cfe8yujp 54 21 ) ) -RRB- cord-291611-cfe8yujp 54 22 and and CC cord-291611-cfe8yujp 54 23 2-min 2-min JJ cord-291611-cfe8yujp 54 24 extention extention NN cord-291611-cfe8yujp 54 25 ( ( -LRB- cord-291611-cfe8yujp 54 26 at at IN cord-291611-cfe8yujp 54 27 72 72 CD cord-291611-cfe8yujp 54 28 " " '' cord-291611-cfe8yujp 54 29 ) ) -RRB- cord-291611-cfe8yujp 54 30 steps step NNS cord-291611-cfe8yujp 54 31 . . . cord-291611-cfe8yujp 55 1 After after IN cord-291611-cfe8yujp 55 2 30 30 CD cord-291611-cfe8yujp 55 3 to to TO cord-291611-cfe8yujp 55 4 35 35 CD cord-291611-cfe8yujp 55 5 cycles cycle NNS cord-291611-cfe8yujp 55 6 , , , cord-291611-cfe8yujp 55 7 the the DT cord-291611-cfe8yujp 55 8 final final JJ cord-291611-cfe8yujp 55 9 products product NNS cord-291611-cfe8yujp 55 10 were be VBD cord-291611-cfe8yujp 55 11 extended extend VBN cord-291611-cfe8yujp 55 12 for for IN cord-291611-cfe8yujp 55 13 7 7 CD cord-291611-cfe8yujp 55 14 min min NN cord-291611-cfe8yujp 55 15 at at IN cord-291611-cfe8yujp 55 16 72 72 CD cord-291611-cfe8yujp 55 17 " " '' cord-291611-cfe8yujp 55 18 . . . cord-291611-cfe8yujp 56 1 Single single JJ cord-291611-cfe8yujp 56 2 - - HYPH cord-291611-cfe8yujp 56 3 strand strand NN cord-291611-cfe8yujp 56 4 cDNA cdna NN cord-291611-cfe8yujp 56 5 fragments fragment NNS cord-291611-cfe8yujp 56 6 were be VBD cord-291611-cfe8yujp 56 7 generated generate VBN cord-291611-cfe8yujp 56 8 in in IN cord-291611-cfe8yujp 56 9 a a DT cord-291611-cfe8yujp 56 10 second second JJ cord-291611-cfe8yujp 56 11 PCR pcr NN cord-291611-cfe8yujp 56 12 for for IN cord-291611-cfe8yujp 56 13 sequencing sequencing NN cord-291611-cfe8yujp 56 14 . . . cord-291611-cfe8yujp 57 1 Briefly briefly RB cord-291611-cfe8yujp 57 2 , , , cord-291611-cfe8yujp 57 3 one one CD cord-291611-cfe8yujp 57 4 - - HYPH cord-291611-cfe8yujp 57 5 tenth tenth NN cord-291611-cfe8yujp 57 6 of of IN cord-291611-cfe8yujp 57 7 the the DT cord-291611-cfe8yujp 57 8 double double JJ cord-291611-cfe8yujp 57 9 stranded strand VBN cord-291611-cfe8yujp 57 10 PCR PCR NNP cord-291611-cfe8yujp 57 11 product product NN cord-291611-cfe8yujp 57 12 and and CC cord-291611-cfe8yujp 57 13 a a DT cord-291611-cfe8yujp 57 14 single single JJ cord-291611-cfe8yujp 57 15 primer primer NN cord-291611-cfe8yujp 57 16 with with IN cord-291611-cfe8yujp 57 17 40 40 CD cord-291611-cfe8yujp 57 18 pmol pmol NN cord-291611-cfe8yujp 57 19 were be VBD cord-291611-cfe8yujp 57 20 used use VBN cord-291611-cfe8yujp 57 21 . . . cord-291611-cfe8yujp 58 1 Other other JJ cord-291611-cfe8yujp 58 2 reagents reagent NNS cord-291611-cfe8yujp 58 3 were be VBD cord-291611-cfe8yujp 58 4 the the DT cord-291611-cfe8yujp 58 5 same same JJ cord-291611-cfe8yujp 58 6 as as IN cord-291611-cfe8yujp 58 7 in in IN cord-291611-cfe8yujp 58 8 the the DT cord-291611-cfe8yujp 58 9 previous previous JJ cord-291611-cfe8yujp 58 10 PCR PCR NNP cord-291611-cfe8yujp 58 11 . . . cord-291611-cfe8yujp 59 1 The the DT cord-291611-cfe8yujp 59 2 cycle cycle NN cord-291611-cfe8yujp 59 3 profile profile NN cord-291611-cfe8yujp 59 4 of of IN cord-291611-cfe8yujp 59 5 20 20 CD cord-291611-cfe8yujp 59 6 to to TO cord-291611-cfe8yujp 59 7 25 25 CD cord-291611-cfe8yujp 59 8 cycles cycle NNS cord-291611-cfe8yujp 59 9 was be VBD cord-291611-cfe8yujp 59 10 : : : cord-291611-cfe8yujp 59 11 1-set 1-set CD cord-291611-cfe8yujp 59 12 at at IN cord-291611-cfe8yujp 59 13 96 96 CD cord-291611-cfe8yujp 59 14 " " NNS cord-291611-cfe8yujp 59 15 , , , cord-291611-cfe8yujp 59 16 30-set 30-set CD cord-291611-cfe8yujp 59 17 at at IN cord-291611-cfe8yujp 59 18 50 50 CD cord-291611-cfe8yujp 59 19 " " '' cord-291611-cfe8yujp 59 20 , , , cord-291611-cfe8yujp 59 21 1-set 1-set CD cord-291611-cfe8yujp 59 22 at at IN cord-291611-cfe8yujp 59 23 60 60 CD cord-291611-cfe8yujp 59 24 " " NNS cord-291611-cfe8yujp 59 25 , , , cord-291611-cfe8yujp 59 26 2-min 2-min JJ cord-291611-cfe8yujp 59 27 at at IN cord-291611-cfe8yujp 59 28 72 72 CD cord-291611-cfe8yujp 59 29 " " '' cord-291611-cfe8yujp 59 30 ( ( -LRB- cord-291611-cfe8yujp 59 31 Fig fig NN cord-291611-cfe8yujp 59 32 . . . cord-291611-cfe8yujp 59 33 1 1 CD cord-291611-cfe8yujp 59 34 ) ) -RRB- cord-291611-cfe8yujp 59 35 . . . cord-291611-cfe8yujp 60 1 We -PRON- PRP cord-291611-cfe8yujp 60 2 sequenced sequence VBD cord-291611-cfe8yujp 60 3 the the DT cord-291611-cfe8yujp 60 4 entire entire JJ cord-291611-cfe8yujp 60 5 S S NNP cord-291611-cfe8yujp 60 6 genes gene NNS cord-291611-cfe8yujp 60 7 of of IN cord-291611-cfe8yujp 60 8 one one CD cord-291611-cfe8yujp 60 9 virulent virulent JJ cord-291611-cfe8yujp 60 10 and and CC cord-291611-cfe8yujp 60 11 two two CD cord-291611-cfe8yujp 60 12 avirulent avirulent NN cord-291611-cfe8yujp 60 13 BCV BCV NNP cord-291611-cfe8yujp 60 14 strains strain NNS cord-291611-cfe8yujp 60 15 which which WDT cord-291611-cfe8yujp 60 16 display display VBP cord-291611-cfe8yujp 60 17 distinct distinct JJ cord-291611-cfe8yujp 60 18 biological biological JJ cord-291611-cfe8yujp 60 19 properties property NNS cord-291611-cfe8yujp 60 20 and and CC cord-291611-cfe8yujp 60 21 epitopes epitope NNS cord-291611-cfe8yujp 60 22 as as IN cord-291611-cfe8yujp 60 23 defined define VBN cord-291611-cfe8yujp 60 24 by by IN cord-291611-cfe8yujp 60 25 MAbs MAbs NNP cord-291611-cfe8yujp 60 26 to to IN cord-291611-cfe8yujp 60 27 the the DT cord-291611-cfe8yujp 60 28 reference reference NN cord-291611-cfe8yujp 60 29 strain strain NN cord-291611-cfe8yujp 60 30 BCV BCV NNP cord-291611-cfe8yujp 60 31 - - HYPH cord-291611-cfe8yujp 60 32 L9 L9 NNP cord-291611-cfe8yujp 60 33 . . . cord-291611-cfe8yujp 61 1 With with IN cord-291611-cfe8yujp 61 2 the the DT cord-291611-cfe8yujp 61 3 exception exception NN cord-291611-cfe8yujp 61 4 of of IN cord-291611-cfe8yujp 61 5 40 40 CD cord-291611-cfe8yujp 61 6 nt nt RB cord-291611-cfe8yujp 61 7 on on IN cord-291611-cfe8yujp 61 8 the the DT cord-291611-cfe8yujp 61 9 5'-end 5'-end CD cord-291611-cfe8yujp 61 10 and and CC cord-291611-cfe8yujp 61 11 50 50 CD cord-291611-cfe8yujp 61 12 nt nt RB cord-291611-cfe8yujp 61 13 on on IN cord-291611-cfe8yujp 61 14 the the DT cord-291611-cfe8yujp 61 15 3'-end 3'-end CD cord-291611-cfe8yujp 61 16 , , , cord-291611-cfe8yujp 61 17 the the DT cord-291611-cfe8yujp 61 18 S S NNP cord-291611-cfe8yujp 61 19 gene gene NN cord-291611-cfe8yujp 61 20 sequences sequence NNS cord-291611-cfe8yujp 61 21 were be VBD cord-291611-cfe8yujp 61 22 determined determine VBN cord-291611-cfe8yujp 61 23 in in IN cord-291611-cfe8yujp 61 24 both both DT cord-291611-cfe8yujp 61 25 directions direction NNS cord-291611-cfe8yujp 61 26 at at IN cord-291611-cfe8yujp 61 27 least least JJS cord-291611-cfe8yujp 61 28 once once RB cord-291611-cfe8yujp 61 29 , , , cord-291611-cfe8yujp 61 30 as as IN cord-291611-cfe8yujp 61 31 illustrated illustrate VBN cord-291611-cfe8yujp 61 32 in in IN cord-291611-cfe8yujp 61 33 Fig Fig NNP cord-291611-cfe8yujp 61 34 . . . cord-291611-cfe8yujp 62 1 1 1 LS cord-291611-cfe8yujp 62 2 . . . cord-291611-cfe8yujp 63 1 These these DT cord-291611-cfe8yujp 63 2 sequences sequence NNS cord-291611-cfe8yujp 63 3 were be VBD cord-291611-cfe8yujp 63 4 compared compare VBN cord-291611-cfe8yujp 63 5 with with IN cord-291611-cfe8yujp 63 6 the the DT cord-291611-cfe8yujp 63 7 recently recently RB cord-291611-cfe8yujp 63 8 published publish VBN cord-291611-cfe8yujp 63 9 sequences sequence NNS cord-291611-cfe8yujp 63 10 for for IN cord-291611-cfe8yujp 63 11 the the DT cord-291611-cfe8yujp 63 12 strains strain NNS cord-291611-cfe8yujp 63 13 BCV BCV NNP cord-291611-cfe8yujp 63 14 - - HYPH cord-291611-cfe8yujp 63 15 Mebus Mebus NNP cord-291611-cfe8yujp 63 16 ( ( -LRB- cord-291611-cfe8yujp 63 17 14 14 CD cord-291611-cfe8yujp 63 18 ) ) -RRB- cord-291611-cfe8yujp 63 19 , , , cord-291611-cfe8yujp 63 20 BCV BCV NNP cord-291611-cfe8yujp 63 21 - - HYPH cord-291611-cfe8yujp 63 22 Quebec Quebec NNP cord-291611-cfe8yujp 63 23 ( ( -LRB- cord-291611-cfe8yujp 63 24 /5 /5 NNP cord-291611-cfe8yujp 63 25 ) ) -RRB- cord-291611-cfe8yujp 63 26 , , , cord-291611-cfe8yujp 63 27 and and CC cord-291611-cfe8yujp 63 28 BCV BCV NNP cord-291611-cfe8yujp 63 29 - - HYPH cord-291611-cfe8yujp 63 30 F15 F15 NNP cord-291611-cfe8yujp 63 31 ( ( -LRB- cord-291611-cfe8yujp 63 32 13 13 CD cord-291611-cfe8yujp 63 33 ) ) -RRB- cord-291611-cfe8yujp 63 34 , , , cord-291611-cfe8yujp 63 35 and and CC cord-291611-cfe8yujp 63 36 their -PRON- PRP$ cord-291611-cfe8yujp 63 37 alignment alignment NN cord-291611-cfe8yujp 63 38 is be VBZ cord-291611-cfe8yujp 63 39 presented present VBN cord-291611-cfe8yujp 63 40 in in IN cord-291611-cfe8yujp 63 41 Fig Fig NNP cord-291611-cfe8yujp 63 42 . . . cord-291611-cfe8yujp 63 43 _SP cord-291611-cfe8yujp 64 1 2 2 LS cord-291611-cfe8yujp 65 1 ( ( -LRB- cord-291611-cfe8yujp 65 2 origins origin NNS cord-291611-cfe8yujp 65 3 of of IN cord-291611-cfe8yujp 65 4 these these DT cord-291611-cfe8yujp 65 5 strains strain NNS cord-291611-cfe8yujp 65 6 are be VBP cord-291611-cfe8yujp 65 7 described describe VBN cord-291611-cfe8yujp 65 8 in in IN cord-291611-cfe8yujp 65 9 the the DT cord-291611-cfe8yujp 65 10 legend legend NN cord-291611-cfe8yujp 65 11 ) ) -RRB- cord-291611-cfe8yujp 65 12 . . . cord-291611-cfe8yujp 66 1 All all DT cord-291611-cfe8yujp 66 2 of of IN cord-291611-cfe8yujp 66 3 the the DT cord-291611-cfe8yujp 66 4 S S NNP cord-291611-cfe8yujp 66 5 genes gene NNS cord-291611-cfe8yujp 66 6 contained contain VBD cord-291611-cfe8yujp 66 7 an an DT cord-291611-cfe8yujp 66 8 ORF ORF NNP cord-291611-cfe8yujp 66 9 of of IN cord-291611-cfe8yujp 66 10 4092 4092 CD cord-291611-cfe8yujp 66 11 nucleotides nucleotide NNS cord-291611-cfe8yujp 66 12 . . . cord-291611-cfe8yujp 67 1 The the DT cord-291611-cfe8yujp 67 2 only only JJ cord-291611-cfe8yujp 67 3 variations variation NNS cord-291611-cfe8yujp 67 4 among among IN cord-291611-cfe8yujp 67 5 these these DT cord-291611-cfe8yujp 67 6 sequences sequence NNS cord-291611-cfe8yujp 67 7 consisted consist VBN cord-291611-cfe8yujp 67 8 of of IN cord-291611-cfe8yujp 67 9 nucleotide nucleotide JJ cord-291611-cfe8yujp 67 10 substitutions substitution NNS cord-291611-cfe8yujp 67 11 . . . cord-291611-cfe8yujp 68 1 Frameshift frameshift NN cord-291611-cfe8yujp 68 2 , , , cord-291611-cfe8yujp 68 3 deletion deletion NN cord-291611-cfe8yujp 68 4 , , , cord-291611-cfe8yujp 68 5 or or CC cord-291611-cfe8yujp 68 6 insertion insertion NN cord-291611-cfe8yujp 68 7 , , , cord-291611-cfe8yujp 68 8 and and CC cord-291611-cfe8yujp 68 9 nonsense nonsense NN cord-291611-cfe8yujp 68 10 mutations mutation NNS cord-291611-cfe8yujp 68 11 were be VBD cord-291611-cfe8yujp 68 12 not not RB cord-291611-cfe8yujp 68 13 observed observe VBN cord-291611-cfe8yujp 68 14 except except IN cord-291611-cfe8yujp 68 15 for for IN cord-291611-cfe8yujp 68 16 BCV BCV NNP cord-291611-cfe8yujp 68 17 - - HYPH cord-291611-cfe8yujp 68 18 F15 F15 NNP cord-291611-cfe8yujp 68 19 . . . cord-291611-cfe8yujp 69 1 The the DT cord-291611-cfe8yujp 69 2 few few JJ cord-291611-cfe8yujp 69 3 nucleotide nucleotide JJ cord-291611-cfe8yujp 69 4 substitutions substitution NNS cord-291611-cfe8yujp 69 5 ( ( -LRB- cord-291611-cfe8yujp 69 6 91 91 CD cord-291611-cfe8yujp 69 7 nt nt NN cord-291611-cfe8yujp 69 8 ) ) -RRB- cord-291611-cfe8yujp 69 9 represented represent VBD cord-291611-cfe8yujp 69 10 approximately approximately RB cord-291611-cfe8yujp 69 11 2 2 CD cord-291611-cfe8yujp 69 12 % % NN cord-291611-cfe8yujp 69 13 of of IN cord-291611-cfe8yujp 69 14 the the DT cord-291611-cfe8yujp 69 15 sequence sequence NN cord-291611-cfe8yujp 69 16 , , , cord-291611-cfe8yujp 69 17 and and CC cord-291611-cfe8yujp 69 18 they -PRON- PRP cord-291611-cfe8yujp 69 19 seemed seem VBD cord-291611-cfe8yujp 69 20 to to TO cord-291611-cfe8yujp 69 21 be be VB cord-291611-cfe8yujp 69 22 distributed distribute VBN cord-291611-cfe8yujp 69 23 randomly randomly RB cord-291611-cfe8yujp 69 24 . . . cord-291611-cfe8yujp 70 1 By by IN cord-291611-cfe8yujp 70 2 comparison comparison NN cord-291611-cfe8yujp 70 3 with with IN cord-291611-cfe8yujp 70 4 other other JJ cord-291611-cfe8yujp 70 5 strains strain NNS cord-291611-cfe8yujp 70 6 , , , cord-291611-cfe8yujp 70 7 the the DT cord-291611-cfe8yujp 70 8 following follow VBG cord-291611-cfe8yujp 70 9 differences difference NNS cord-291611-cfe8yujp 70 10 were be VBD cord-291611-cfe8yujp 70 11 detected detect VBN cord-291611-cfe8yujp 70 12 in in IN cord-291611-cfe8yujp 70 13 two two CD cord-291611-cfe8yujp 70 14 regions region NNS cord-291611-cfe8yujp 70 15 in in IN cord-291611-cfe8yujp 70 16 BCV BCV NNP cord-291611-cfe8yujp 70 17 - - HYPH cord-291611-cfe8yujp 70 18 F15 F15 NNP cord-291611-cfe8yujp 70 19 : : : cord-291611-cfe8yujp 71 1 The the DT cord-291611-cfe8yujp 71 2 deletion deletion NN cord-291611-cfe8yujp 71 3 of of IN cord-291611-cfe8yujp 71 4 a a DT cord-291611-cfe8yujp 71 5 base base NN cord-291611-cfe8yujp 71 6 ( ( -LRB- cord-291611-cfe8yujp 71 7 T T NNP cord-291611-cfe8yujp 71 8 ) ) -RRB- cord-291611-cfe8yujp 71 9 at at IN cord-291611-cfe8yujp 71 10 nucleotide nucleotide JJ cord-291611-cfe8yujp 71 11 2316 2316 CD cord-291611-cfe8yujp 71 12 resulted result VBD cord-291611-cfe8yujp 71 13 in in IN cord-291611-cfe8yujp 71 14 a a DT cord-291611-cfe8yujp 71 15 frameshift frameshift NN cord-291611-cfe8yujp 71 16 for for IN cord-291611-cfe8yujp 71 17 2 2 CD cord-291611-cfe8yujp 71 18 aa aa NNP cord-291611-cfe8yujp 71 19 followed follow VBN cord-291611-cfe8yujp 71 20 by by IN cord-291611-cfe8yujp 71 21 the the DT cord-291611-cfe8yujp 71 22 insertion insertion NN cord-291611-cfe8yujp 71 23 of of IN cord-291611-cfe8yujp 71 24 an an DT cord-291611-cfe8yujp 71 25 A a NN cord-291611-cfe8yujp 71 26 at at IN cord-291611-cfe8yujp 71 27 nucleotide nucleotide JJ cord-291611-cfe8yujp 71 28 2320 2320 CD cord-291611-cfe8yujp 71 29 which which WDT cord-291611-cfe8yujp 71 30 returned return VBD cord-291611-cfe8yujp 71 31 the the DT cord-291611-cfe8yujp 71 32 amino amino NN cord-291611-cfe8yujp 71 33 acid acid NN cord-291611-cfe8yujp 71 34 sequence sequence NN cord-291611-cfe8yujp 71 35 to to IN cord-291611-cfe8yujp 71 36 a a DT cord-291611-cfe8yujp 71 37 homologous homologous JJ cord-291611-cfe8yujp 71 38 alignment alignment NN cord-291611-cfe8yujp 71 39 ( ( -LRB- cord-291611-cfe8yujp 71 40 at at IN cord-291611-cfe8yujp 71 41 aa aa NNP cord-291611-cfe8yujp 71 42 position position NN cord-291611-cfe8yujp 71 43 772 772 CD cord-291611-cfe8yujp 71 44 within within IN cord-291611-cfe8yujp 71 45 the the DT cord-291611-cfe8yujp 71 46 cleavage cleavage NN cord-291611-cfe8yujp 71 47 site site NN cord-291611-cfe8yujp 71 48 ; ; : cord-291611-cfe8yujp 71 49 also also RB cord-291611-cfe8yujp 71 50 see see VB cord-291611-cfe8yujp 71 51 second second JJ cord-291611-cfe8yujp 71 52 - - HYPH cord-291611-cfe8yujp 71 53 strand strand NN cord-291611-cfe8yujp 71 54 , , , cord-291611-cfe8yujp 71 55 PCR pcr NN cord-291611-cfe8yujp 71 56 - - HYPH cord-291611-cfe8yujp 71 57 mediated mediate VBN cord-291611-cfe8yujp 71 58 DNA dna NN cord-291611-cfe8yujp 71 59 synthesis synthesis NN cord-291611-cfe8yujp 71 60 ( ( -LRB- cord-291611-cfe8yujp 71 61 c c NN cord-291611-cfe8yujp 71 62 ) ) -RRB- cord-291611-cfe8yujp 71 63 ; ; : cord-291611-cfe8yujp 71 64 double double RB cord-291611-cfe8yujp 71 65 - - HYPH cord-291611-cfe8yujp 71 66 stranded strand VBN cord-291611-cfe8yujp 71 67 DNA dna NN cord-291611-cfe8yujp 71 68 amplification amplification NN cord-291611-cfe8yujp 71 69 by by IN cord-291611-cfe8yujp 71 70 PCR PCR NNP cord-291611-cfe8yujp 71 71 ( ( -LRB- cord-291611-cfe8yujp 71 72 d d NN cord-291611-cfe8yujp 71 73 ) ) -RRB- cord-291611-cfe8yujp 71 74 ; ; : cord-291611-cfe8yujp 71 75 single single RB cord-291611-cfe8yujp 71 76 - - HYPH cord-291611-cfe8yujp 71 77 stranded strand VBN cord-291611-cfe8yujp 71 78 DNA dna NN cord-291611-cfe8yujp 71 79 synthesis synthesis NN cord-291611-cfe8yujp 71 80 by by IN cord-291611-cfe8yujp 71 81 PCR PCR NNP cord-291611-cfe8yujp 71 82 using use VBG cord-291611-cfe8yujp 71 83 an an DT cord-291611-cfe8yujp 71 84 excess excess NN cord-291611-cfe8yujp 71 85 of of IN cord-291611-cfe8yujp 71 86 5 5 CD cord-291611-cfe8yujp 71 87 ' ' '' cord-291611-cfe8yujp 71 88 primer primer NN cord-291611-cfe8yujp 71 89 ( ( -LRB- cord-291611-cfe8yujp 71 90 e e NN cord-291611-cfe8yujp 71 91 ) ) -RRB- cord-291611-cfe8yujp 71 92 and and CC cord-291611-cfe8yujp 71 93 3 3 CD cord-291611-cfe8yujp 71 94 ' ' `` cord-291611-cfe8yujp 71 95 primer primer NN cord-291611-cfe8yujp 71 96 ( ( -LRB- cord-291611-cfe8yujp 71 97 f f NNP cord-291611-cfe8yujp 71 98 ) ) -RRB- cord-291611-cfe8yujp 71 99 ; ; : cord-291611-cfe8yujp 71 100 DNA dna NN cord-291611-cfe8yujp 71 101 sequencing sequencing NN cord-291611-cfe8yujp 71 102 strategies(g strategies(g NN cord-291611-cfe8yujp 71 103 ) ) -RRB- cord-291611-cfe8yujp 71 104 . . . cord-291611-cfe8yujp 72 1 A5'and a5'and CD cord-291611-cfe8yujp 72 2 85 85 CD cord-291611-cfe8yujp 72 3 ' ' NNS cord-291611-cfe8yujp 72 4 indicate indicate VBP cord-291611-cfe8yujp 72 5 two two CD cord-291611-cfe8yujp 72 6 5'primers 5'primers CD cord-291611-cfe8yujp 72 7 containing contain VBG cord-291611-cfe8yujp 72 8 an an DT cord-291611-cfe8yujp 72 9 extra extra JJ cord-291611-cfe8yujp 72 10 EcoRl ecorl NN cord-291611-cfe8yujp 72 11 linker linker NN cord-291611-cfe8yujp 72 12 ; ; : cord-291611-cfe8yujp 72 13 A3 A3 NNP cord-291611-cfe8yujp 72 14 ' ' POS cord-291611-cfe8yujp 72 15 and and CC cord-291611-cfe8yujp 72 16 B3 B3 NNP cord-291611-cfe8yujp 72 17 ' ' '' cord-291611-cfe8yujp 72 18 indicate indicate VBP cord-291611-cfe8yujp 72 19 two two CD cord-291611-cfe8yujp 72 20 3 3 CD cord-291611-cfe8yujp 72 21 ' ' POS cord-291611-cfe8yujp 72 22 primers primer NNS cord-291611-cfe8yujp 72 23 containing contain VBG cord-291611-cfe8yujp 72 24 an an DT cord-291611-cfe8yujp 72 25 extra extra JJ cord-291611-cfe8yujp 72 26 BarnHI BarnHI NNP cord-291611-cfe8yujp 72 27 linker linker NN cord-291611-cfe8yujp 72 28 . . . cord-291611-cfe8yujp 73 1 ( ( -LRB- cord-291611-cfe8yujp 73 2 13 13 CD cord-291611-cfe8yujp 73 3 ) ) -RRB- cord-291611-cfe8yujp 73 4 , , , cord-291611-cfe8yujp 73 5 respectively respectively RB cord-291611-cfe8yujp 73 6 . . . cord-291611-cfe8yujp 74 1 The the DT cord-291611-cfe8yujp 74 2 origins origin NNS cord-291611-cfe8yujp 74 3 and and CC cord-291611-cfe8yujp 74 4 properties property NNS cord-291611-cfe8yujp 74 5 of of IN cord-291611-cfe8yujp 74 6 these these DT cord-291611-cfe8yujp 74 7 strains strain NNS cord-291611-cfe8yujp 74 8 are be VBP cord-291611-cfe8yujp 74 9 as as IN cord-291611-cfe8yujp 74 10 follows follow VBZ cord-291611-cfe8yujp 74 11 : : : cord-291611-cfe8yujp 75 1 The the DT cord-291611-cfe8yujp 75 2 strain strain NN cord-291611-cfe8yujp 75 3 BCV BCV NNP cord-291611-cfe8yujp 75 4 - - HYPH cord-291611-cfe8yujp 75 5 Mebus Mebus NNP cord-291611-cfe8yujp 75 6 was be VBD cord-291611-cfe8yujp 75 7 isolated isolate VBN cord-291611-cfe8yujp 75 8 from from IN cord-291611-cfe8yujp 75 9 a a DT cord-291611-cfe8yujp 75 10 calf calf NN cord-291611-cfe8yujp 75 11 with with IN cord-291611-cfe8yujp 75 12 enteritis enteritis NN cord-291611-cfe8yujp 75 13 , , , cord-291611-cfe8yujp 75 14 and and CC cord-291611-cfe8yujp 75 15 adapted adapt VBN cord-291611-cfe8yujp 75 16 to to IN cord-291611-cfe8yujp 75 17 bovine bovine JJ cord-291611-cfe8yujp 75 18 fetal fetal JJ cord-291611-cfe8yujp 75 19 kidney kidney NN cord-291611-cfe8yujp 75 20 cells cell NNS cord-291611-cfe8yujp 75 21 described describe VBN cord-291611-cfe8yujp 75 22 previously previously RB cord-291611-cfe8yujp 75 23 ( ( -LRB- cord-291611-cfe8yujp 75 24 25 25 CD cord-291611-cfe8yujp 75 25 ) ) -RRB- cord-291611-cfe8yujp 75 26 . . . cord-291611-cfe8yujp 76 1 The the DT cord-291611-cfe8yujp 76 2 strain strain NN cord-291611-cfe8yujp 76 3 BCV BCV NNP cord-291611-cfe8yujp 76 4 - - HYPH cord-291611-cfe8yujp 76 5 Quebec Quebec NNP cord-291611-cfe8yujp 76 6 was be VBD cord-291611-cfe8yujp 76 7 isolated isolate VBN cord-291611-cfe8yujp 76 8 from from IN cord-291611-cfe8yujp 76 9 a a DT cord-291611-cfe8yujp 76 10 calf calf NN cord-291611-cfe8yujp 76 11 with with IN cord-291611-cfe8yujp 76 12 enteritis enteritis NN cord-291611-cfe8yujp 76 13 by by IN cord-291611-cfe8yujp 76 14 cultivation cultivation NN cord-291611-cfe8yujp 76 15 in in IN cord-291611-cfe8yujp 76 16 Vero Vero NNP cord-291611-cfe8yujp 76 17 and and CC cord-291611-cfe8yujp 76 18 MDBK MDBK NNP cord-291611-cfe8yujp 76 19 cells cell NNS cord-291611-cfe8yujp 76 20 in in IN cord-291611-cfe8yujp 76 21 Quebec Quebec NNP cord-291611-cfe8yujp 76 22 in in IN cord-291611-cfe8yujp 76 23 1979 1979 CD cord-291611-cfe8yujp 76 24 . . . cord-291611-cfe8yujp 77 1 Its -PRON- PRP$ cord-291611-cfe8yujp 77 2 biological biological JJ cord-291611-cfe8yujp 77 3 properties property NNS cord-291611-cfe8yujp 77 4 were be VBD cord-291611-cfe8yujp 77 5 similar similar JJ cord-291611-cfe8yujp 77 6 to to IN cord-291611-cfe8yujp 77 7 BCV BCV NNP cord-291611-cfe8yujp 77 8 - - HYPH cord-291611-cfe8yujp 77 9 Mebus Mebus NNP cord-291611-cfe8yujp 77 10 ( ( -LRB- cord-291611-cfe8yujp 77 11 26 26 CD cord-291611-cfe8yujp 77 12 ) ) -RRB- cord-291611-cfe8yujp 77 13 . . . cord-291611-cfe8yujp 78 1 The the DT cord-291611-cfe8yujp 78 2 wild wild JJ cord-291611-cfe8yujp 78 3 - - HYPH cord-291611-cfe8yujp 78 4 type type NN cord-291611-cfe8yujp 78 5 strain strain NN cord-291611-cfe8yujp 78 6 BCVF15 BCVF15 NNP cord-291611-cfe8yujp 78 7 was be VBD cord-291611-cfe8yujp 78 8 isolated isolate VBN cord-291611-cfe8yujp 78 9 in in IN cord-291611-cfe8yujp 78 10 HRT HRT NNP cord-291611-cfe8yujp 78 11 cells cell NNS cord-291611-cfe8yujp 78 12 from from IN cord-291611-cfe8yujp 78 13 a a DT cord-291611-cfe8yujp 78 14 calf calf NN cord-291611-cfe8yujp 78 15 with with IN cord-291611-cfe8yujp 78 16 enteritis enteritis NN cord-291611-cfe8yujp 78 17 in in IN cord-291611-cfe8yujp 78 18 France France NNP cord-291611-cfe8yujp 78 19 in in IN cord-291611-cfe8yujp 78 20 1979 1979 CD cord-291611-cfe8yujp 78 21 ( ( -LRB- cord-291611-cfe8yujp 78 22 27 27 CD cord-291611-cfe8yujp 78 23 ) ) -RRB- cord-291611-cfe8yujp 78 24 . . . cord-291611-cfe8yujp 79 1 The the DT cord-291611-cfe8yujp 79 2 consensus consensus NN cord-291611-cfe8yujp 79 3 sequences sequence NNS cord-291611-cfe8yujp 79 4 are be VBP cord-291611-cfe8yujp 79 5 underlined underline VBN cord-291611-cfe8yujp 79 6 , , , cord-291611-cfe8yujp 79 7 and and CC cord-291611-cfe8yujp 79 8 the the DT cord-291611-cfe8yujp 79 9 start start NN cord-291611-cfe8yujp 79 10 and and CC cord-291611-cfe8yujp 79 11 stop stop VB cord-291611-cfe8yujp 79 12 codes code NNS cord-291611-cfe8yujp 79 13 are be VBP cord-291611-cfe8yujp 79 14 marked mark VBN cord-291611-cfe8yujp 79 15 by by IN cord-291611-cfe8yujp 79 16 asterisks asterisk NNS cord-291611-cfe8yujp 79 17 . . . cord-291611-cfe8yujp 80 1 most most RBS cord-291611-cfe8yujp 80 2 similar similar JJ cord-291611-cfe8yujp 80 3 sequences sequence NNS cord-291611-cfe8yujp 80 4 were be VBD cord-291611-cfe8yujp 80 5 those those DT cord-291611-cfe8yujp 80 6 of of IN cord-291611-cfe8yujp 80 7 BCV BCV NNP cord-291611-cfe8yujp 80 8 - - HYPH cord-291611-cfe8yujp 80 9 L9 L9 NNP cord-291611-cfe8yujp 80 10 and and CC cord-291611-cfe8yujp 80 11 BCV BCV NNP cord-291611-cfe8yujp 80 12 - - HYPH cord-291611-cfe8yujp 80 13 Vaccine Vaccine NNP cord-291611-cfe8yujp 80 14 ( ( -LRB- cord-291611-cfe8yujp 80 15 6 6 CD cord-291611-cfe8yujp 80 16 nt nt RB cord-291611-cfe8yujp 80 17 differences difference NNS cord-291611-cfe8yujp 80 18 ) ) -RRB- cord-291611-cfe8yujp 80 19 in in IN cord-291611-cfe8yujp 80 20 paired pair VBN cord-291611-cfe8yujp 80 21 comparisons comparison NNS cord-291611-cfe8yujp 80 22 , , , cord-291611-cfe8yujp 80 23 and and CC cord-291611-cfe8yujp 80 24 the the DT cord-291611-cfe8yujp 80 25 most most RBS cord-291611-cfe8yujp 80 26 distant distant JJ cord-291611-cfe8yujp 80 27 were be VBD cord-291611-cfe8yujp 80 28 those those DT cord-291611-cfe8yujp 80 29 of of IN cord-291611-cfe8yujp 80 30 BCV BCV NNP cord-291611-cfe8yujp 80 31 - - HYPH cord-291611-cfe8yujp 80 32 Quebec Quebec NNP cord-291611-cfe8yujp 80 33 and and CC cord-291611-cfe8yujp 80 34 BCV BCV NNP cord-291611-cfe8yujp 80 35 - - HYPH cord-291611-cfe8yujp 80 36 F15 F15 NNP cord-291611-cfe8yujp 80 37 ( ( -LRB- cord-291611-cfe8yujp 80 38 74 74 CD cord-291611-cfe8yujp 80 39 nt nt RB cord-291611-cfe8yujp 80 40 differences difference NNS cord-291611-cfe8yujp 80 41 ) ) -RRB- cord-291611-cfe8yujp 80 42 . . . cord-291611-cfe8yujp 81 1 The the DT cord-291611-cfe8yujp 81 2 virulent virulent JJ cord-291611-cfe8yujp 81 3 wild wild JJ cord-291611-cfe8yujp 81 4 - - HYPH cord-291611-cfe8yujp 81 5 type type NN cord-291611-cfe8yujp 81 6 strain strain NN cord-291611-cfe8yujp 82 1 BCV BCV NNP cord-291611-cfe8yujp 82 2 - - HYPH cord-291611-cfe8yujp 82 3 LY138 LY138 NNP cord-291611-cfe8yujp 82 4 was be VBD cord-291611-cfe8yujp 82 5 evolutionally evolutionally RB cord-291611-cfe8yujp 82 6 distant distant JJ cord-291611-cfe8yujp 82 7 from from IN cord-291611-cfe8yujp 82 8 the the DT cord-291611-cfe8yujp 82 9 avirulent avirulent NN cord-291611-cfe8yujp 82 10 strains strain NNS cord-291611-cfe8yujp 82 11 ( ( -LRB- cord-291611-cfe8yujp 82 12 total total JJ cord-291611-cfe8yujp 82 13 number number NN cord-291611-cfe8yujp 82 14 of of IN cord-291611-cfe8yujp 82 15 differences difference NNS cord-291611-cfe8yujp 82 16 between between IN cord-291611-cfe8yujp 82 17 45 45 CD cord-291611-cfe8yujp 82 18 to to TO cord-291611-cfe8yujp 82 19 56 56 CD cord-291611-cfe8yujp 82 20 nt nt RB cord-291611-cfe8yujp 82 21 ) ) -RRB- cord-291611-cfe8yujp 82 22 . . . cord-291611-cfe8yujp 83 1 The the DT cord-291611-cfe8yujp 83 2 French french JJ cord-291611-cfe8yujp 83 3 strain strain NN cord-291611-cfe8yujp 84 1 BCV BCV NNP cord-291611-cfe8yujp 84 2 - - HYPH cord-291611-cfe8yujp 84 3 F15 F15 NNP cord-291611-cfe8yujp 84 4 also also RB cord-291611-cfe8yujp 84 5 differed differ VBD cord-291611-cfe8yujp 84 6 from from IN cord-291611-cfe8yujp 84 7 the the DT cord-291611-cfe8yujp 84 8 avirulent avirulent NN cord-291611-cfe8yujp 84 9 strains strain NNS cord-291611-cfe8yujp 84 10 ( ( -LRB- cord-291611-cfe8yujp 84 11 total total JJ cord-291611-cfe8yujp 84 12 number number NN cord-291611-cfe8yujp 84 13 of of IN cord-291611-cfe8yujp 84 14 differences difference NNS cord-291611-cfe8yujp 84 15 between between IN cord-291611-cfe8yujp 84 16 55 55 CD cord-291611-cfe8yujp 84 17 to to TO cord-291611-cfe8yujp 84 18 74 74 CD cord-291611-cfe8yujp 84 19 nt nt NN cord-291611-cfe8yujp 84 20 ) ) -RRB- cord-291611-cfe8yujp 84 21 . . . cord-291611-cfe8yujp 85 1 Although although IN cord-291611-cfe8yujp 85 2 there there EX cord-291611-cfe8yujp 85 3 were be VBD cord-291611-cfe8yujp 85 4 55 55 CD cord-291611-cfe8yujp 85 5 nt nt RB cord-291611-cfe8yujp 85 6 differences difference NNS cord-291611-cfe8yujp 85 7 between between IN cord-291611-cfe8yujp 85 8 BCV BCV NNP cord-291611-cfe8yujp 85 9 - - HYPH cord-291611-cfe8yujp 85 10 LY138 LY138 NNP cord-291611-cfe8yujp 85 11 and and CC cord-291611-cfe8yujp 85 12 BCV BCV NNP cord-291611-cfe8yujp 85 13 - - HYPH cord-291611-cfe8yujp 85 14 F15 F15 NNP cord-291611-cfe8yujp 85 15 , , , cord-291611-cfe8yujp 85 16 the the DT cord-291611-cfe8yujp 85 17 same same JJ cord-291611-cfe8yujp 85 18 nucleotide nucleotide JJ cord-291611-cfe8yujp 85 19 substitutions substitution NNS cord-291611-cfe8yujp 85 20 occurred occur VBD cord-291611-cfe8yujp 85 21 in in IN cord-291611-cfe8yujp 85 22 29 29 CD cord-291611-cfe8yujp 85 23 nt nt RB cord-291611-cfe8yujp 85 24 . . . cord-291611-cfe8yujp 86 1 An an DT cord-291611-cfe8yujp 86 2 alignment alignment NN cord-291611-cfe8yujp 86 3 of of IN cord-291611-cfe8yujp 86 4 the the DT cord-291611-cfe8yujp 86 5 deduced deduce VBN cord-291611-cfe8yujp 86 6 amino amino NN cord-291611-cfe8yujp 86 7 acid acid NN cord-291611-cfe8yujp 86 8 sequences sequence NNS cord-291611-cfe8yujp 86 9 of of IN cord-291611-cfe8yujp 86 10 the the DT cord-291611-cfe8yujp 86 11 S S NNP cord-291611-cfe8yujp 86 12 proteins protein NNS cord-291611-cfe8yujp 86 13 of of IN cord-291611-cfe8yujp 86 14 the the DT cord-291611-cfe8yujp 86 15 six six CD cord-291611-cfe8yujp 86 16 BCV BCV NNP cord-291611-cfe8yujp 86 17 strains strain NNS cord-291611-cfe8yujp 86 18 is be VBZ cord-291611-cfe8yujp 86 19 shown show VBN cord-291611-cfe8yujp 86 20 in in IN cord-291611-cfe8yujp 86 21 Fig Fig NNP cord-291611-cfe8yujp 86 22 . . . cord-291611-cfe8yujp 86 23 _SP cord-291611-cfe8yujp 87 1 3 3 LS cord-291611-cfe8yujp 87 2 . . . cord-291611-cfe8yujp 88 1 All all DT cord-291611-cfe8yujp 88 2 six six CD cord-291611-cfe8yujp 88 3 S S NNP cord-291611-cfe8yujp 88 4 genes gene NNS cord-291611-cfe8yujp 88 5 encoded encode VBD cord-291611-cfe8yujp 88 6 a a DT cord-291611-cfe8yujp 88 7 predicted predict VBN cord-291611-cfe8yujp 88 8 protein protein NN cord-291611-cfe8yujp 88 9 of of IN cord-291611-cfe8yujp 88 10 1363 1363 CD cord-291611-cfe8yujp 88 11 aa aa NNP cord-291611-cfe8yujp 88 12 residues residue NNS cord-291611-cfe8yujp 88 13 , , , cord-291611-cfe8yujp 88 14 having have VBG cord-291611-cfe8yujp 88 15 a a DT cord-291611-cfe8yujp 88 16 mol mol NN cord-291611-cfe8yujp 88 17 . . . cord-291611-cfe8yujp 89 1 wt wt NN cord-291611-cfe8yujp 89 2 . . NNP cord-291611-cfe8yujp 89 3 of of IN cord-291611-cfe8yujp 89 4 approximately approximately RB cord-291611-cfe8yujp 89 5 150 150 CD cord-291611-cfe8yujp 89 6 kDa kDa NNS cord-291611-cfe8yujp 89 7 . . . cord-291611-cfe8yujp 90 1 Nineteen nineteen CD cord-291611-cfe8yujp 90 2 potential potential JJ cord-291611-cfe8yujp 90 3 N n CD cord-291611-cfe8yujp 90 4 - - HYPH cord-291611-cfe8yujp 90 5 linked link VBN cord-291611-cfe8yujp 90 6 glycosylation glycosylation NN cord-291611-cfe8yujp 90 7 sites site NNS cord-291611-cfe8yujp 90 8 were be VBD cord-291611-cfe8yujp 90 9 predicted predict VBN cord-291611-cfe8yujp 90 10 in in IN cord-291611-cfe8yujp 90 11 5 5 CD cord-291611-cfe8yujp 90 12 strains strain NNS cord-291611-cfe8yujp 90 13 while while IN cord-291611-cfe8yujp 90 14 18 18 CD cord-291611-cfe8yujp 90 15 of of IN cord-291611-cfe8yujp 90 16 them -PRON- PRP cord-291611-cfe8yujp 90 17 were be VBD cord-291611-cfe8yujp 90 18 conserved conserve VBN cord-291611-cfe8yujp 90 19 in in IN cord-291611-cfe8yujp 90 20 BCV BCV NNP cord-291611-cfe8yujp 90 21 - - HYPH cord-291611-cfe8yujp 90 22 L9 L9 NNP cord-291611-cfe8yujp 90 23 . . . cord-291611-cfe8yujp 91 1 Fifty fifty CD cord-291611-cfe8yujp 91 2 - - HYPH cord-291611-cfe8yujp 91 3 seven seven CD cord-291611-cfe8yujp 91 4 cysteine cysteine NN cord-291611-cfe8yujp 91 5 residues residue NNS cord-291611-cfe8yujp 91 6 were be VBD cord-291611-cfe8yujp 91 7 found find VBN cord-291611-cfe8yujp 91 8 in in IN cord-291611-cfe8yujp 91 9 BCV BCV NNP cord-291611-cfe8yujp 91 10 - - HYPH cord-291611-cfe8yujp 91 11 L9 L9 NNP cord-291611-cfe8yujp 91 12 while while IN cord-291611-cfe8yujp 91 13 56 56 CD cord-291611-cfe8yujp 91 14 of of IN cord-291611-cfe8yujp 91 15 them -PRON- PRP cord-291611-cfe8yujp 91 16 were be VBD cord-291611-cfe8yujp 91 17 conserved conserve VBN cord-291611-cfe8yujp 91 18 in in IN cord-291611-cfe8yujp 91 19 the the DT cord-291611-cfe8yujp 91 20 other other JJ cord-291611-cfe8yujp 91 21 5 5 CD cord-291611-cfe8yujp 91 22 strains strain NNS cord-291611-cfe8yujp 91 23 . . . cord-291611-cfe8yujp 92 1 The the DT cord-291611-cfe8yujp 92 2 BCV BCV NNP cord-291611-cfe8yujp 92 3 - - HYPH cord-291611-cfe8yujp 92 4 F15 F15 NNP cord-291611-cfe8yujp 92 5 had have VBD cord-291611-cfe8yujp 92 6 an an DT cord-291611-cfe8yujp 92 7 additional additional JJ cord-291611-cfe8yujp 92 8 cysteine cysteine NN cord-291611-cfe8yujp 92 9 at at IN cord-291611-cfe8yujp 92 10 position position NN cord-291611-cfe8yujp 92 11 840 840 CD cord-291611-cfe8yujp 92 12 . . . cord-291611-cfe8yujp 93 1 The the DT cord-291611-cfe8yujp 93 2 glycosylated glycosylated JJ cord-291611-cfe8yujp 93 3 protein protein NN cord-291611-cfe8yujp 93 4 had have VBD cord-291611-cfe8yujp 93 5 an an DT cord-291611-cfe8yujp 93 6 estimated estimate VBN cord-291611-cfe8yujp 93 7 mol mol NN cord-291611-cfe8yujp 93 8 . . . cord-291611-cfe8yujp 94 1 wt wt NN cord-291611-cfe8yujp 94 2 . . NNP cord-291611-cfe8yujp 94 3 of of IN cord-291611-cfe8yujp 94 4 approximately approximately RB cord-291611-cfe8yujp 94 5 190 190 CD cord-291611-cfe8yujp 94 6 kDa kDa NNS cord-291611-cfe8yujp 94 7 . . . cord-291611-cfe8yujp 95 1 All all DT cord-291611-cfe8yujp 95 2 six six CD cord-291611-cfe8yujp 95 3 S s NN cord-291611-cfe8yujp 95 4 proteins protein NNS cord-291611-cfe8yujp 95 5 possessed possess VBD cord-291611-cfe8yujp 95 6 at at IN cord-291611-cfe8yujp 95 7 least least RBS cord-291611-cfe8yujp 95 8 98 98 CD cord-291611-cfe8yujp 95 9 % % NN cord-291611-cfe8yujp 95 10 sequence sequence NN cord-291611-cfe8yujp 95 11 identity identity NN cord-291611-cfe8yujp 95 12 in in IN cord-291611-cfe8yujp 95 13 paired pair VBN cord-291611-cfe8yujp 95 14 comparisons comparison NNS cord-291611-cfe8yujp 95 15 . . . cord-291611-cfe8yujp 96 1 As as IN cord-291611-cfe8yujp 96 2 with with IN cord-291611-cfe8yujp 96 3 the the DT cord-291611-cfe8yujp 96 4 nucleotide nucleotide NN cord-291611-cfe8yujp 96 5 se- se- NN cord-291611-cfe8yujp 96 6 Scv Scv NNP cord-291611-cfe8yujp 96 7 - - HYPH cord-291611-cfe8yujp 96 8 L9 L9 NNP cord-291611-cfe8yujp 96 9 _SP cord-291611-cfe8yujp 96 10 LDHNLNMFLI LDHNLNMFLI NNP cord-291611-cfe8yujp 96 11 LLISLPMAFA LLISLPMAFA NNP cord-291611-cfe8yujp 96 12 VIGDLKCTTV VIGDLKCTTV NNP cord-291611-cfe8yujp 96 13 SINDVDTGU SINDVDTGU NNP cord-291611-cfe8yujp 96 14 _SP cord-291611-cfe8yujp 97 1 TYYVLD TYYVLD NNP cord-291611-cfe8yujp 97 2 RVYLNITLLL RVYLNITLLL NNP cord-291611-cfe8yujp 97 3 NGWPTSCST NGWPTSCST NNP cord-291611-cfe8yujp 97 4 YRNMALKGTL YRNMALKGTL NNP cord-291611-cfe8yujp 97 5 LLSRLWTKPP LLSRLWTKPP NNP cord-291611-cfe8yujp 97 6 _SP cord-291611-cfe8yujp 98 1 BCV BCV NNP cord-291611-cfe8yujp 98 2 - - HYPH cord-291611-cfe8yujp 98 3 VACC VACC NNP cord-291611-cfe8yujp 98 4 _SP cord-291611-cfe8yujp 99 1 SISTD$m sistd$m NN cord-291611-cfe8yujp 99 2 _SP cord-291611-cfe8yujp 100 1 NGLG NGLG NNP cord-291611-cfe8yujp 100 2 _SP cord-291611-cfe8yujp 100 3 -BCV -bcv ADD cord-291611-cfe8yujp 101 1 -MEB -MEB VBN cord-291611-cfe8yujp 101 2 _SP cord-291611-cfe8yujp 101 3 EN EN NNP cord-291611-cfe8yujp 101 4 WEB web NN cord-291611-cfe8yujp 101 5 _SP cord-291611-cfe8yujp 102 1 -2 -2 NNP cord-291611-cfe8yujp 102 2 _SP cord-291611-cfe8yujp 103 1 Bcv Bcv NNP cord-291611-cfe8yujp 103 2 - - HYPH cord-291611-cfe8yujp 103 3 Y Y NNP cord-291611-cfe8yujp 103 4 _SP cord-291611-cfe8yujp 104 1 V V NNP cord-291611-cfe8yujp 104 2 _SP cord-291611-cfe8yujp 105 1 BCV BCV NNP cord-291611-cfe8yujp 105 2 - - HYPH cord-291611-cfe8yujp 105 3 F15 F15 NNP cord-291611-cfe8yujp 105 4 _SP cord-291611-cfe8yujp 105 5 : : : cord-291611-cfe8yujp 105 6 _SP cord-291611-cfe8yujp 105 7 T T NNP cord-291611-cfe8yujp 105 8 _SP cord-291611-cfe8yujp 105 9 T T NNP cord-291611-cfe8yujp 105 10 _SP cord-291611-cfe8yujp 105 11 BCV BCV NNP cord-291611-cfe8yujp 105 12 - - HYPH cord-291611-cfe8yujp 105 13 L9 L9 NNP cord-291611-cfe8yujp 105 14 _SP cord-291611-cfe8yujp 106 1 ECV ECV NNP cord-291611-cfe8yujp 106 2 - - HYPH cord-291611-cfe8yujp 106 3 VACC VACC NNP cord-291611-cfe8yujp 106 4 _SP cord-291611-cfe8yujp 107 1 PLSDFINGIF PLSDFINGIF NNP cord-291611-cfe8yujp 107 2 AKVKNTKVIK AKVKNTKVIK NNP cord-291611-cfe8yujp 108 1 KGVNYSEPPA KGVNYSEPPA NNP cord-291611-cfe8yujp 108 2 ITIGSTFVNT ITIGSTFVNT NNP cord-291611-cfe8yujp 108 3 SYSWVQPHT SYSWVQPHT NNP cord-291611-cfe8yujp 108 4 _SP cord-291611-cfe8yujp 109 1 TNLDNKLQGL TNLDNKLQGL NNP cord-291611-cfe8yujp 109 2 LEISVCQYTM LEISVCQYTM NNP cord-291611-cfe8yujp 109 3 CEYPBTICBP CEYPBTICBP NNP cord-291611-cfe8yujp 110 1 NI&yVEW NI&yVEW NNP cord-291611-cfe8yujp 110 2 _SP cord-291611-cfe8yujp 111 1 RWDXVVSCL RWDXVVSCL NNP cord-291611-cfe8yujp 111 2 _SP cord-291611-cfe8yujp 112 1 I= I= NNP cord-291611-cfe8yujp 112 2 _SP cord-291611-cfe8yujp 113 1 BCV BCV NNP cord-291611-cfe8yujp 113 2 - - HYPH cord-291611-cfe8yujp 113 3 MEB MEB NNP cord-291611-cfe8yujp 113 4 _SP cord-291611-cfe8yujp 113 5 BCV BCV NNP cord-291611-cfe8yujp 113 6 quences quence VBZ cord-291611-cfe8yujp 113 7 , , , cord-291611-cfe8yujp 113 8 the the DT cord-291611-cfe8yujp 113 9 most most RBS cord-291611-cfe8yujp 113 10 related related JJ cord-291611-cfe8yujp 113 11 proteins protein NNS cord-291611-cfe8yujp 113 12 were be VBD cord-291611-cfe8yujp 113 13 those those DT cord-291611-cfe8yujp 113 14 of of IN cord-291611-cfe8yujp 113 15 BCVgent bcvgent NN cord-291611-cfe8yujp 113 16 proteins protein NNS cord-291611-cfe8yujp 113 17 were be VBD cord-291611-cfe8yujp 113 18 those those DT cord-291611-cfe8yujp 113 19 of of IN cord-291611-cfe8yujp 113 20 BCV BCV NNP cord-291611-cfe8yujp 113 21 - - HYPH cord-291611-cfe8yujp 113 22 Mebus Mebus NNP cord-291611-cfe8yujp 113 23 and and CC cord-291611-cfe8yujp 113 24 BCV BCV NNP cord-291611-cfe8yujp 113 25 - - HYPH cord-291611-cfe8yujp 113 26 F15 F15 NNP cord-291611-cfe8yujp 113 27 Mebus Mebus NNP cord-291611-cfe8yujp 113 28 and and CC cord-291611-cfe8yujp 113 29 BCV BCV NNP cord-291611-cfe8yujp 113 30 - - HYPH cord-291611-cfe8yujp 113 31 Quebec Quebec NNP cord-291611-cfe8yujp 113 32 ( ( -LRB- cord-291611-cfe8yujp 113 33 4 4 CD cord-291611-cfe8yujp 113 34 aa aa NNP cord-291611-cfe8yujp 113 35 differences difference NNS cord-291611-cfe8yujp 113 36 ) ) -RRB- cord-291611-cfe8yujp 113 37 or or CC cord-291611-cfe8yujp 113 38 BCV BCV NNP cord-291611-cfe8yujp 113 39 - - HYPH cord-291611-cfe8yujp 113 40 L9 L9 NNP cord-291611-cfe8yujp 113 41 or or CC cord-291611-cfe8yujp 113 42 BCV BCV NNP cord-291611-cfe8yujp 113 43 - - HYPH cord-291611-cfe8yujp 113 44 Quebec Quebec NNP cord-291611-cfe8yujp 113 45 and and CC cord-291611-cfe8yujp 113 46 BCV BCV NNP cord-291611-cfe8yujp 113 47 - - HYPH cord-291611-cfe8yujp 113 48 F15 F15 NNP cord-291611-cfe8yujp 113 49 ( ( -LRB- cord-291611-cfe8yujp 113 50 26 26 CD cord-291611-cfe8yujp 113 51 aa aa NNP cord-291611-cfe8yujp 113 52 differences difference NNS cord-291611-cfe8yujp 113 53 , , , cord-291611-cfe8yujp 113 54 reand reand NNP cord-291611-cfe8yujp 113 55 BCV BCV NNP cord-291611-cfe8yujp 113 56 - - HYPH cord-291611-cfe8yujp 113 57 Vaccine Vaccine NNP cord-291611-cfe8yujp 113 58 ( ( -LRB- cord-291611-cfe8yujp 113 59 6 6 CD cord-291611-cfe8yujp 113 60 aa aa NNP cord-291611-cfe8yujp 113 61 differences difference NNS cord-291611-cfe8yujp 113 62 ) ) -RRB- cord-291611-cfe8yujp 113 63 ; ; : cord-291611-cfe8yujp 113 64 the the DT cord-291611-cfe8yujp 113 65 most most RBS cord-291611-cfe8yujp 113 66 diverspectively diverspectively JJ cord-291611-cfe8yujp 113 67 ) ) -RRB- cord-291611-cfe8yujp 113 68 . . . cord-291611-cfe8yujp 114 1 The the DT cord-291611-cfe8yujp 114 2 alignment alignment NN cord-291611-cfe8yujp 114 3 reveals reveal VBZ cord-291611-cfe8yujp 114 4 that that IN cord-291611-cfe8yujp 114 5 the the DT cord-291611-cfe8yujp 114 6 strain strain NN cord-291611-cfe8yujp 114 7 BCV BCV NNP cord-291611-cfe8yujp 114 8 - - HYPH cord-291611-cfe8yujp 114 9 Quebec Quebec NNP cord-291611-cfe8yujp 114 10 is be VBZ cord-291611-cfe8yujp 114 11 closely closely RB cord-291611-cfe8yujp 114 12 related related JJ cord-291611-cfe8yujp 114 13 to to IN cord-291611-cfe8yujp 114 14 the the DT cord-291611-cfe8yujp 114 15 avirulent avirulent NN cord-291611-cfe8yujp 114 16 strains strain NNS cord-291611-cfe8yujp 115 1 BCV BCV NNP cord-291611-cfe8yujp 115 2 - - HYPH cord-291611-cfe8yujp 115 3 Mebus Mebus NNP cord-291611-cfe8yujp 115 4 , , , cord-291611-cfe8yujp 115 5 BCV BCV NNP cord-291611-cfe8yujp 115 6 - - HYPH cord-291611-cfe8yujp 115 7 L9 L9 NNP cord-291611-cfe8yujp 115 8 , , , cord-291611-cfe8yujp 115 9 and and CC cord-291611-cfe8yujp 115 10 BCV BCV NNP cord-291611-cfe8yujp 115 11 - - HYPH cord-291611-cfe8yujp 115 12 Vaccine Vaccine NNP cord-291611-cfe8yujp 115 13 ( ( -LRB- cord-291611-cfe8yujp 115 14 6 6 CD cord-291611-cfe8yujp 115 15 to to TO cord-291611-cfe8yujp 115 16 14 14 CD cord-291611-cfe8yujp 115 17 nt nt RB cord-291611-cfe8yujp 115 18 and and CC cord-291611-cfe8yujp 115 19 4 4 CD cord-291611-cfe8yujp 115 20 to to TO cord-291611-cfe8yujp 115 21 11 11 CD cord-291611-cfe8yujp 115 22 aa aa NNP cord-291611-cfe8yujp 115 23 differences difference NNS cord-291611-cfe8yujp 115 24 , , , cord-291611-cfe8yujp 115 25 respectively respectively RB cord-291611-cfe8yujp 115 26 ) ) -RRB- cord-291611-cfe8yujp 115 27 , , . cord-291611-cfe8yujp 116 1 suggesting suggest VBG cord-291611-cfe8yujp 116 2 that that IN cord-291611-cfe8yujp 116 3 this this DT cord-291611-cfe8yujp 116 4 strain strain NN cord-291611-cfe8yujp 116 5 is be VBZ cord-291611-cfe8yujp 116 6 a a DT cord-291611-cfe8yujp 116 7 variant variant NN cord-291611-cfe8yujp 116 8 of of IN cord-291611-cfe8yujp 116 9 the the DT cord-291611-cfe8yujp 116 10 prototype prototype NN cord-291611-cfe8yujp 116 11 BCV BCV NNP cord-291611-cfe8yujp 116 12 - - HYPH cord-291611-cfe8yujp 116 13 Mebus Mebus NNP cord-291611-cfe8yujp 116 14 . . . cord-291611-cfe8yujp 117 1 In in IN cord-291611-cfe8yujp 117 2 contrast contrast NN cord-291611-cfe8yujp 117 3 to to IN cord-291611-cfe8yujp 117 4 the the DT cord-291611-cfe8yujp 117 5 nucleotide nucleotide JJ cord-291611-cfe8yujp 117 6 sequences sequence NNS cord-291611-cfe8yujp 117 7 , , , cord-291611-cfe8yujp 117 8 the the DT cord-291611-cfe8yujp 117 9 amino amino NN cord-291611-cfe8yujp 117 10 acid acid NN cord-291611-cfe8yujp 117 11 sequences sequence NNS cord-291611-cfe8yujp 117 12 of of IN cord-291611-cfe8yujp 117 13 BCV BCV NNP cord-291611-cfe8yujp 117 14 - - HYPH cord-291611-cfe8yujp 117 15 LY138 LY138 NNP cord-291611-cfe8yujp 117 16 and and CC cord-291611-cfe8yujp 117 17 BCV BCV NNP cord-291611-cfe8yujp 117 18 - - HYPH cord-291611-cfe8yujp 117 19 F15 F15 NNP cord-291611-cfe8yujp 117 20 were be VBD cord-291611-cfe8yujp 117 21 more more RBR cord-291611-cfe8yujp 117 22 homologous homologous JJ cord-291611-cfe8yujp 117 23 ( ( -LRB- cord-291611-cfe8yujp 117 24 13 13 CD cord-291611-cfe8yujp 117 25 aa aa NNP cord-291611-cfe8yujp 117 26 differences difference NNS cord-291611-cfe8yujp 117 27 ) ) -RRB- cord-291611-cfe8yujp 117 28 . . . cord-291611-cfe8yujp 118 1 As as IN cord-291611-cfe8yujp 118 2 shown show VBN cord-291611-cfe8yujp 118 3 in in IN cord-291611-cfe8yujp 118 4 Fig Fig NNP cord-291611-cfe8yujp 118 5 . . . cord-291611-cfe8yujp 119 1 3 3 CD cord-291611-cfe8yujp 119 2 , , , cord-291611-cfe8yujp 119 3 9 9 CD cord-291611-cfe8yujp 119 4 amino amino NN cord-291611-cfe8yujp 119 5 acids acid NNS cord-291611-cfe8yujp 119 6 in in IN cord-291611-cfe8yujp 119 7 the the DT cord-291611-cfe8yujp 119 8 avirulent avirulent NN cord-291611-cfe8yujp 119 9 strains strain NNS cord-291611-cfe8yujp 119 10 were be VBD cord-291611-cfe8yujp 119 11 substituted substitute VBN cord-291611-cfe8yujp 119 12 in in IN cord-291611-cfe8yujp 119 13 both both DT cord-291611-cfe8yujp 119 14 BCV BCV NNP cord-291611-cfe8yujp 119 15 - - HYPH cord-291611-cfe8yujp 119 16 LY138 LY138 NNP cord-291611-cfe8yujp 119 17 and and CC cord-291611-cfe8yujp 119 18 BCV BCV NNP cord-291611-cfe8yujp 119 19 - - HYPH cord-291611-cfe8yujp 119 20 F15 F15 NNP cord-291611-cfe8yujp 119 21 : : : cord-291611-cfe8yujp 120 1 A a NN cord-291611-cfe8yujp 120 2 to to IN cord-291611-cfe8yujp 120 3 V v NN cord-291611-cfe8yujp 120 4 ( ( -LRB- cord-291611-cfe8yujp 120 5 38 38 CD cord-291611-cfe8yujp 120 6 ) ) -RRB- cord-291611-cfe8yujp 120 7 , , , cord-291611-cfe8yujp 120 8 R r NN cord-291611-cfe8yujp 120 9 to to IN cord-291611-cfe8yujp 120 10 T t NN cord-291611-cfe8yujp 121 1 ( ( -LRB- cord-291611-cfe8yujp 121 2 94),KtoN(121),LtoM(254),HtoD(476),VtoA 94),kton(121),ltom(254),htod(476),vtoa CD cord-291611-cfe8yujp 121 3 ( ( -LRB- cord-291611-cfe8yujp 121 4 749),VtoE(980),AtoV 749),vtoe(980),atov CD cord-291611-cfe8yujp 121 5 ( ( -LRB- cord-291611-cfe8yujp 121 6 1106),HtoP(1247),andI 1106),htop(1247),andi CD cord-291611-cfe8yujp 121 7 to to IN cord-291611-cfe8yujp 121 8 K K NNP cord-291611-cfe8yujp 121 9 ( ( -LRB- cord-291611-cfe8yujp 121 10 1347 1347 CD cord-291611-cfe8yujp 121 11 ) ) -RRB- cord-291611-cfe8yujp 121 12 . . . cord-291611-cfe8yujp 122 1 This this DT cord-291611-cfe8yujp 122 2 suggests suggest VBZ cord-291611-cfe8yujp 122 3 that that IN cord-291611-cfe8yujp 122 4 the the DT cord-291611-cfe8yujp 122 5 wild wild JJ cord-291611-cfe8yujp 122 6 - - HYPH cord-291611-cfe8yujp 122 7 type type NN cord-291611-cfe8yujp 122 8 strain strain NN cord-291611-cfe8yujp 122 9 BCV BCV NNP cord-291611-cfe8yujp 122 10 - - HYPH cord-291611-cfe8yujp 122 11 F15 F15 NNP cord-291611-cfe8yujp 122 12 is be VBZ cord-291611-cfe8yujp 122 13 possibly possibly RB cord-291611-cfe8yujp 122 14 a a DT cord-291611-cfe8yujp 122 15 virulent virulent JJ cord-291611-cfe8yujp 122 16 strain strain NN cord-291611-cfe8yujp 122 17 , , , cord-291611-cfe8yujp 122 18 and and CC cord-291611-cfe8yujp 122 19 that that IN cord-291611-cfe8yujp 122 20 the the DT cord-291611-cfe8yujp 122 21 9 9 CD cord-291611-cfe8yujp 122 22 amino amino NN cord-291611-cfe8yujp 122 23 acids acid NNS cord-291611-cfe8yujp 122 24 , , , cord-291611-cfe8yujp 122 25 especially especially RB cord-291611-cfe8yujp 122 26 the the DT cord-291611-cfe8yujp 122 27 proline proline NN cord-291611-cfe8yujp 122 28 ( ( -LRB- cord-291611-cfe8yujp 122 29 at at IN cord-291611-cfe8yujp 122 30 1247 1247 CD cord-291611-cfe8yujp 122 31 ) ) -RRB- cord-291611-cfe8yujp 122 32 and and CC cord-291611-cfe8yujp 122 33 lysine lysine NN cord-291611-cfe8yujp 122 34 ( ( -LRB- cord-291611-cfe8yujp 122 35 at at IN cord-291611-cfe8yujp 122 36 1347 1347 CD cord-291611-cfe8yujp 122 37 ) ) -RRB- cord-291611-cfe8yujp 122 38 on on IN cord-291611-cfe8yujp 122 39 the the DT cord-291611-cfe8yujp 122 40 carboxy carboxy JJ cord-291611-cfe8yujp 122 41 - - HYPH cord-291611-cfe8yujp 122 42 terminal terminal NN cord-291611-cfe8yujp 122 43 , , , cord-291611-cfe8yujp 122 44 may may MD cord-291611-cfe8yujp 122 45 relate relate VB cord-291611-cfe8yujp 122 46 to to IN cord-291611-cfe8yujp 122 47 BCV BCV NNP cord-291611-cfe8yujp 122 48 virulence virulence NN cord-291611-cfe8yujp 122 49 . . . cord-291611-cfe8yujp 123 1 Amino amino NN cord-291611-cfe8yujp 123 2 acid acid NN cord-291611-cfe8yujp 123 3 differences difference NNS cord-291611-cfe8yujp 123 4 between between IN cord-291611-cfe8yujp 123 5 the the DT cord-291611-cfe8yujp 123 6 virulent virulent JJ cord-291611-cfe8yujp 123 7 and and CC cord-291611-cfe8yujp 123 8 avirulent avirulent JJ cord-291611-cfe8yujp 123 9 groups group NNS cord-291611-cfe8yujp 123 10 varied vary VBN cord-291611-cfe8yujp 123 11 from from IN cord-291611-cfe8yujp 123 12 16 16 CD cord-291611-cfe8yujp 123 13 to to TO cord-291611-cfe8yujp 123 14 26 26 CD cord-291611-cfe8yujp 123 15 aa aa NN cord-291611-cfe8yujp 123 16 . . . cord-291611-cfe8yujp 124 1 Apparently apparently RB cord-291611-cfe8yujp 124 2 , , , cord-291611-cfe8yujp 124 3 most most JJS cord-291611-cfe8yujp 124 4 of of IN cord-291611-cfe8yujp 124 5 these these DT cord-291611-cfe8yujp 124 6 substitutions substitution NNS cord-291611-cfe8yujp 124 7 occurred occur VBD cord-291611-cfe8yujp 124 8 in in IN cord-291611-cfe8yujp 124 9 the the DT cord-291611-cfe8yujp 124 10 Sl sl NN cord-291611-cfe8yujp 124 11 peptide peptide NN cord-291611-cfe8yujp 124 12 ( ( -LRB- cord-291611-cfe8yujp 124 13 Fig Fig NNP cord-291611-cfe8yujp 124 14 . . . cord-291611-cfe8yujp 124 15 3 3 CD cord-291611-cfe8yujp 124 16 ) ) -RRB- cord-291611-cfe8yujp 124 17 . . . cord-291611-cfe8yujp 125 1 Comparisons comparison NNS cord-291611-cfe8yujp 125 2 of of IN cord-291611-cfe8yujp 125 3 the the DT cord-291611-cfe8yujp 125 4 amino amino NN cord-291611-cfe8yujp 125 5 acid acid NN cord-291611-cfe8yujp 125 6 sequences sequence NNS cord-291611-cfe8yujp 125 7 in in IN cord-291611-cfe8yujp 125 8 the the DT cord-291611-cfe8yujp 125 9 antigenic antigenic JJ cord-291611-cfe8yujp 125 10 region region NN cord-291611-cfe8yujp 125 11 ( ( -LRB- cord-291611-cfe8yujp 125 12 14 14 CD cord-291611-cfe8yujp 125 13 , , , cord-291611-cfe8yujp 125 14 16 16 CD cord-291611-cfe8yujp 125 15 ) ) -RRB- cord-291611-cfe8yujp 125 16 suggested suggest VBD cord-291611-cfe8yujp 125 17 that that IN cord-291611-cfe8yujp 125 18 two two CD cord-291611-cfe8yujp 125 19 proline proline NN cord-291611-cfe8yujp 125 20 substitutions substitution NNS cord-291611-cfe8yujp 125 21 may may MD cord-291611-cfe8yujp 125 22 cause cause VB cord-291611-cfe8yujp 125 23 the the DT cord-291611-cfe8yujp 125 24 antigenic antigenic JJ cord-291611-cfe8yujp 125 25 differences difference NNS cord-291611-cfe8yujp 125 26 : : : cord-291611-cfe8yujp 126 1 at at IN cord-291611-cfe8yujp 126 2 amino amino NN cord-291611-cfe8yujp 126 3 acid acid NN cord-291611-cfe8yujp 126 4 position position NN cord-291611-cfe8yujp 126 5 507 507 CD cord-291611-cfe8yujp 126 6 , , , cord-291611-cfe8yujp 126 7 a a DT cord-291611-cfe8yujp 126 8 proline proline NN cord-291611-cfe8yujp 126 9 in in IN cord-291611-cfe8yujp 126 10 the the DT cord-291611-cfe8yujp 126 11 avirulent avirulent NN cord-291611-cfe8yujp 126 12 strain strain NN cord-291611-cfe8yujp 126 13 BCV BCV NNP cord-291611-cfe8yujp 126 14 - - HYPH cord-291611-cfe8yujp 126 15 L9 L9 NNP cord-291611-cfe8yujp 126 16 was be VBD cord-291611-cfe8yujp 126 17 substituted substitute VBN cord-291611-cfe8yujp 126 18 by by IN cord-291611-cfe8yujp 126 19 a a DT cord-291611-cfe8yujp 126 20 serine serine NN cord-291611-cfe8yujp 126 21 in in IN cord-291611-cfe8yujp 126 22 the the DT cord-291611-cfe8yujp 126 23 virulent virulent JJ cord-291611-cfe8yujp 126 24 strain strain NN cord-291611-cfe8yujp 126 25 BCV BCV NNP cord-291611-cfe8yujp 126 26 - - HYPH cord-291611-cfe8yujp 126 27 LY138 LY138 NNP cord-291611-cfe8yujp 126 28 , , , cord-291611-cfe8yujp 126 29 and and CC cord-291611-cfe8yujp 126 30 another another DT cord-291611-cfe8yujp 126 31 proline proline NN cord-291611-cfe8yujp 126 32 ( ( -LRB- cord-291611-cfe8yujp 126 33 at at IN cord-291611-cfe8yujp 126 34 567 567 CD cord-291611-cfe8yujp 126 35 ) ) -RRB- cord-291611-cfe8yujp 126 36 was be VBD cord-291611-cfe8yujp 126 37 substituted substitute VBN cord-291611-cfe8yujp 126 38 by by IN cord-291611-cfe8yujp 126 39 a a DT cord-291611-cfe8yujp 126 40 threonine threonine NN cord-291611-cfe8yujp 126 41 in in IN cord-291611-cfe8yujp 126 42 all all DT cord-291611-cfe8yujp 126 43 other other JJ cord-291611-cfe8yujp 126 44 strains strain NNS cord-291611-cfe8yujp 126 45 . . . cord-291611-cfe8yujp 127 1 Proline Proline NNP cord-291611-cfe8yujp 127 2 is be VBZ cord-291611-cfe8yujp 127 3 considered consider VBN cord-291611-cfe8yujp 127 4 a a DT cord-291611-cfe8yujp 127 5 helix helix NN cord-291611-cfe8yujp 127 6 - - HYPH cord-291611-cfe8yujp 127 7 breaking break VBG cord-291611-cfe8yujp 127 8 residue residue NN cord-291611-cfe8yujp 127 9 , , , cord-291611-cfe8yujp 127 10 which which WDT cord-291611-cfe8yujp 127 11 may may MD cord-291611-cfe8yujp 127 12 influence influence VB cord-291611-cfe8yujp 127 13 significantly significantly RB cord-291611-cfe8yujp 127 14 the the DT cord-291611-cfe8yujp 127 15 conformation conformation NN cord-291611-cfe8yujp 127 16 and and CC cord-291611-cfe8yujp 127 17 secondary secondary JJ cord-291611-cfe8yujp 127 18 structure structure NN cord-291611-cfe8yujp 127 19 of of IN cord-291611-cfe8yujp 127 20 peptide peptide NN cord-291611-cfe8yujp 127 21 molecules molecule NNS cord-291611-cfe8yujp 127 22 ( ( -LRB- cord-291611-cfe8yujp 127 23 17 17 CD cord-291611-cfe8yujp 127 24 ) ) -RRB- cord-291611-cfe8yujp 127 25 . . . cord-291611-cfe8yujp 128 1 The the DT cord-291611-cfe8yujp 128 2 proline proline NN cord-291611-cfe8yujp 128 3 substitutions substitution NNS cord-291611-cfe8yujp 128 4 resulted result VBD cord-291611-cfe8yujp 128 5 in in IN cord-291611-cfe8yujp 128 6 decreased decrease VBN cord-291611-cfe8yujp 128 7 hydrophilicity hydrophilicity NN cord-291611-cfe8yujp 128 8 , , , cord-291611-cfe8yujp 128 9 surface surface NN cord-291611-cfe8yujp 128 10 probability probability NN cord-291611-cfe8yujp 128 11 , , , cord-291611-cfe8yujp 128 12 and and CC cord-291611-cfe8yujp 128 13 antigenicity antigenicity NN cord-291611-cfe8yujp 128 14 , , , cord-291611-cfe8yujp 128 15 but but CC cord-291611-cfe8yujp 128 16 increased increase VBN cord-291611-cfe8yujp 128 17 flexibility flexibility NN cord-291611-cfe8yujp 128 18 of of IN cord-291611-cfe8yujp 128 19 the the DT cord-291611-cfe8yujp 128 20 peptide peptide NN cord-291611-cfe8yujp 128 21 in in IN cord-291611-cfe8yujp 128 22 this this DT cord-291611-cfe8yujp 128 23 region region NN cord-291611-cfe8yujp 128 24 ( ( -LRB- cord-291611-cfe8yujp 128 25 data datum NNS cord-291611-cfe8yujp 128 26 not not RB cord-291611-cfe8yujp 128 27 shown show VBN cord-291611-cfe8yujp 128 28 ) ) -RRB- cord-291611-cfe8yujp 128 29 . . . cord-291611-cfe8yujp 129 1 Our -PRON- PRP$ cord-291611-cfe8yujp 129 2 previous previous JJ cord-291611-cfe8yujp 129 3 study study NN cord-291611-cfe8yujp 129 4 indicated indicate VBD cord-291611-cfe8yujp 129 5 that that IN cord-291611-cfe8yujp 129 6 one one CD cord-291611-cfe8yujp 129 7 neutralizing neutralize VBG cord-291611-cfe8yujp 129 8 epitope epitope NN cord-291611-cfe8yujp 129 9 of of IN cord-291611-cfe8yujp 129 10 BCV BCV NNP cord-291611-cfe8yujp 129 11 - - HYPH cord-291611-cfe8yujp 129 12 L9 L9 NNP cord-291611-cfe8yujp 129 13 was be VBD cord-291611-cfe8yujp 129 14 present present JJ cord-291611-cfe8yujp 129 15 in in IN cord-291611-cfe8yujp 129 16 BCV BCV NNP cord-291611-cfe8yujp 129 17 - - HYPH cord-291611-cfe8yujp 129 18 Vaccine Vaccine NNP cord-291611-cfe8yujp 129 19 but but CC cord-291611-cfe8yujp 129 20 absent absent JJ cord-291611-cfe8yujp 129 21 in in IN cord-291611-cfe8yujp 129 22 the the DT cord-291611-cfe8yujp 129 23 virulent virulent JJ cord-291611-cfe8yujp 129 24 strain strain NN cord-291611-cfe8yujp 129 25 BCV BCV NNP cord-291611-cfe8yujp 129 26 - - HYPH cord-291611-cfe8yujp 129 27 LY LY NNP cord-291611-cfe8yujp 129 28 138 138 CD cord-291611-cfe8yujp 129 29 , , , cord-291611-cfe8yujp 129 30 and and CC cord-291611-cfe8yujp 129 31 one one CD cord-291611-cfe8yujp 129 32 non non JJ cord-291611-cfe8yujp 129 33 - - JJ cord-291611-cfe8yujp 129 34 neutralizing neutralizing JJ cord-291611-cfe8yujp 129 35 epitope epitope NN cord-291611-cfe8yujp 129 36 was be VBD cord-291611-cfe8yujp 129 37 present present JJ cord-291611-cfe8yujp 129 38 only only RB cord-291611-cfe8yujp 129 39 in in IN cord-291611-cfe8yujp 129 40 the the DT cord-291611-cfe8yujp 129 41 avirulent avirulent NN cord-291611-cfe8yujp 129 42 strain strain NN cord-291611-cfe8yujp 129 43 BCV BCV NNP cord-291611-cfe8yujp 129 44 - - HYPH cord-291611-cfe8yujp 129 45 L9 L9 NNP cord-291611-cfe8yujp 129 46 ( ( -LRB- cord-291611-cfe8yujp 129 47 Hussain Hussain NNP cord-291611-cfe8yujp 129 48 et et NNP cord-291611-cfe8yujp 129 49 a/. a/. NNP cord-291611-cfe8yujp 129 50 , , , cord-291611-cfe8yujp 129 51 submitted submit VBD cord-291611-cfe8yujp 129 52 ) ) -RRB- cord-291611-cfe8yujp 129 53 . . . cord-291611-cfe8yujp 130 1 The the DT cord-291611-cfe8yujp 130 2 number number NN cord-291611-cfe8yujp 130 3 of of IN cord-291611-cfe8yujp 130 4 proline proline NN cord-291611-cfe8yujp 130 5 substitutions substitution NNS cord-291611-cfe8yujp 130 6 in in IN cord-291611-cfe8yujp 130 7 this this DT cord-291611-cfe8yujp 130 8 region region NN cord-291611-cfe8yujp 130 9 coincides coincide VBZ cord-291611-cfe8yujp 130 10 with with IN cord-291611-cfe8yujp 130 11 the the DT cord-291611-cfe8yujp 130 12 number number NN cord-291611-cfe8yujp 130 13 of of IN cord-291611-cfe8yujp 130 14 conformational conformational JJ cord-291611-cfe8yujp 130 15 epitopes epitopes NN cord-291611-cfe8yujp 130 16 absent absent JJ cord-291611-cfe8yujp 130 17 in in IN cord-291611-cfe8yujp 130 18 BCV BCV NNP cord-291611-cfe8yujp 130 19 - - HYPH cord-291611-cfe8yujp 130 20 LY LY NNP cord-291611-cfe8yujp 130 21 138 138 CD cord-291611-cfe8yujp 130 22 and and CC cord-291611-cfe8yujp 130 23 BCV BCV NNP cord-291611-cfe8yujp 130 24 - - HYPH cord-291611-cfe8yujp 130 25 Vaccine Vaccine NNP cord-291611-cfe8yujp 130 26 lead lead VBP cord-291611-cfe8yujp 130 27 us -PRON- PRP cord-291611-cfe8yujp 130 28 to to TO cord-291611-cfe8yujp 130 29 conclude conclude VB cord-291611-cfe8yujp 130 30 that that IN cord-291611-cfe8yujp 130 31 substitutions substitution NNS cord-291611-cfe8yujp 130 32 of of IN cord-291611-cfe8yujp 130 33 these these DT cord-291611-cfe8yujp 130 34 prolines proline NNS cord-291611-cfe8yujp 130 35 to to IN cord-291611-cfe8yujp 130 36 other other JJ cord-291611-cfe8yujp 130 37 amino amino NN cord-291611-cfe8yujp 130 38 acids acid NNS cord-291611-cfe8yujp 130 39 may may MD cord-291611-cfe8yujp 130 40 alter alter VB cord-291611-cfe8yujp 130 41 the the DT cord-291611-cfe8yujp 130 42 conformation conformation NN cord-291611-cfe8yujp 130 43 of of IN cord-291611-cfe8yujp 130 44 the the DT cord-291611-cfe8yujp 130 45 S S NNP cord-291611-cfe8yujp 130 46 protein protein NN cord-291611-cfe8yujp 130 47 , , , cord-291611-cfe8yujp 130 48 and and CC cord-291611-cfe8yujp 130 49 cause cause VB cord-291611-cfe8yujp 130 50 the the DT cord-291611-cfe8yujp 130 51 loss loss NN cord-291611-cfe8yujp 130 52 of of IN cord-291611-cfe8yujp 130 53 conformation conformation NN cord-291611-cfe8yujp 130 54 - - HYPH cord-291611-cfe8yujp 130 55 dependent dependent JJ cord-291611-cfe8yujp 130 56 epitopes epitope NNS cord-291611-cfe8yujp 130 57 in in IN cord-291611-cfe8yujp 130 58 BCV BCV NNP cord-291611-cfe8yujp 130 59 - - HYPH cord-291611-cfe8yujp 130 60 LY138 LY138 NNP cord-291611-cfe8yujp 130 61 and and CC cord-291611-cfe8yujp 130 62 BVC BVC NNP cord-291611-cfe8yujp 130 63 - - HYPH cord-291611-cfe8yujp 130 64 Vaccine Vaccine NNP cord-291611-cfe8yujp 130 65 . . . cord-291611-cfe8yujp 131 1 This this DT cord-291611-cfe8yujp 131 2 hypothesis hypothesis NN cord-291611-cfe8yujp 131 3 is be VBZ cord-291611-cfe8yujp 131 4 currently currently RB cord-291611-cfe8yujp 131 5 under under IN cord-291611-cfe8yujp 131 6 investigation investigation NN cord-291611-cfe8yujp 131 7 . . . cord-291611-cfe8yujp 132 1 It -PRON- PRP cord-291611-cfe8yujp 132 2 is be VBZ cord-291611-cfe8yujp 132 3 unclear unclear JJ cord-291611-cfe8yujp 132 4 whether whether IN cord-291611-cfe8yujp 132 5 substitutions substitution NNS cord-291611-cfe8yujp 132 6 of of IN cord-291611-cfe8yujp 132 7 these these DT cord-291611-cfe8yujp 132 8 prolines proline NNS cord-291611-cfe8yujp 132 9 and and CC cord-291611-cfe8yujp 132 10 other other JJ cord-291611-cfe8yujp 132 11 amino amino NN cord-291611-cfe8yujp 132 12 acids acid NNS cord-291611-cfe8yujp 132 13 in in IN cord-291611-cfe8yujp 132 14 this this DT cord-291611-cfe8yujp 132 15 region region NN cord-291611-cfe8yujp 132 16 also also RB cord-291611-cfe8yujp 132 17 correlate correlate VBP cord-291611-cfe8yujp 132 18 with with IN cord-291611-cfe8yujp 132 19 BCV BCV NNP cord-291611-cfe8yujp 132 20 virulence virulence NN cord-291611-cfe8yujp 132 21 . . . cord-291611-cfe8yujp 133 1 Our -PRON- PRP$ cord-291611-cfe8yujp 133 2 previous previous JJ cord-291611-cfe8yujp 133 3 studies study NNS cord-291611-cfe8yujp 133 4 revealed reveal VBD cord-291611-cfe8yujp 133 5 that that IN cord-291611-cfe8yujp 133 6 the the DT cord-291611-cfe8yujp 133 7 biological biological JJ cord-291611-cfe8yujp 133 8 properties property NNS cord-291611-cfe8yujp 133 9 of of IN cord-291611-cfe8yujp 133 10 fusion fusion NN cord-291611-cfe8yujp 133 11 , , , cord-291611-cfe8yujp 133 12 plaque plaque NN cord-291611-cfe8yujp 133 13 formation formation NN cord-291611-cfe8yujp 133 14 and and CC cord-291611-cfe8yujp 133 15 host host NN cord-291611-cfe8yujp 133 16 cell cell NN cord-291611-cfe8yujp 133 17 range range NN cord-291611-cfe8yujp 133 18 were be VBD cord-291611-cfe8yujp 133 19 evidently evidently RB cord-291611-cfe8yujp 133 20 different different JJ cord-291611-cfe8yujp 133 21 between between IN cord-291611-cfe8yujp 133 22 the the DT cord-291611-cfe8yujp 133 23 virulent virulent JJ cord-291611-cfe8yujp 133 24 and and CC cord-291611-cfe8yujp 133 25 avirulent avirulent JJ cord-291611-cfe8yujp 133 26 groups group NNS cord-291611-cfe8yujp 133 27 of of IN cord-291611-cfe8yujp 133 28 BCV BCV NNP cord-291611-cfe8yujp 133 29 strains strain NNS cord-291611-cfe8yujp 133 30 ( ( -LRB- cord-291611-cfe8yujp 133 31 8) 8) NNP cord-291611-cfe8yujp 133 32 . . . cord-291611-cfe8yujp 134 1 The the DT cord-291611-cfe8yujp 134 2 fusion fusion NN cord-291611-cfe8yujp 134 3 activity activity NN cord-291611-cfe8yujp 134 4 of of IN cord-291611-cfe8yujp 134 5 BCV BCV NNP cord-291611-cfe8yujp 134 6 is be VBZ cord-291611-cfe8yujp 134 7 believed believe VBN cord-291611-cfe8yujp 134 8 to to TO cord-291611-cfe8yujp 134 9 be be VB cord-291611-cfe8yujp 134 10 associated associate VBN cord-291611-cfe8yujp 134 11 with with IN cord-291611-cfe8yujp 134 12 the the DT cord-291611-cfe8yujp 134 13 S S NNP cord-291611-cfe8yujp 134 14 polypeptide polypeptide NN cord-291611-cfe8yujp 134 15 and and CC cord-291611-cfe8yujp 134 16 the the DT cord-291611-cfe8yujp 134 17 cleavage cleavage NN cord-291611-cfe8yujp 134 18 of of IN cord-291611-cfe8yujp 134 19 the the DT cord-291611-cfe8yujp 134 20 S S NNP cord-291611-cfe8yujp 134 21 into into IN cord-291611-cfe8yujp 134 22 Sl Sl NNP cord-291611-cfe8yujp 134 23 and and CC cord-291611-cfe8yujp 134 24 S2 s2 NN cord-291611-cfe8yujp 134 25 subunits subunit NNS cord-291611-cfe8yujp 134 26 ( ( -LRB- cord-291611-cfe8yujp 134 27 5 5 CD cord-291611-cfe8yujp 134 28 , , , cord-291611-cfe8yujp 134 29 18 18 CD cord-291611-cfe8yujp 134 30 ) ) -RRB- cord-291611-cfe8yujp 134 31 . . . cord-291611-cfe8yujp 135 1 The the DT cord-291611-cfe8yujp 135 2 sequence sequence NN cord-291611-cfe8yujp 135 3 KRRSRR KRRSRR NNP cord-291611-cfe8yujp 135 4 at at IN cord-291611-cfe8yujp 135 5 the the DT cord-291611-cfe8yujp 135 6 predicted predict VBN cord-291611-cfe8yujp 135 7 proteo proteo NN cord-291611-cfe8yujp 135 8 - - HYPH cord-291611-cfe8yujp 135 9 Iytic iytic JJ cord-291611-cfe8yujp 135 10 cleavage cleavage NN cord-291611-cfe8yujp 135 11 site site NN cord-291611-cfe8yujp 135 12 was be VBD cord-291611-cfe8yujp 135 13 conserved conserve VBN cord-291611-cfe8yujp 135 14 in in IN cord-291611-cfe8yujp 135 15 the the DT cord-291611-cfe8yujp 135 16 virulent virulent JJ cord-291611-cfe8yujp 135 17 strain strain NN cord-291611-cfe8yujp 135 18 BCV BCV NNP cord-291611-cfe8yujp 135 19 - - HYPH cord-291611-cfe8yujp 135 20 LY138 LY138 NNP cord-291611-cfe8yujp 135 21 and and CC cord-291611-cfe8yujp 135 22 in in IN cord-291611-cfe8yujp 135 23 the the DT cord-291611-cfe8yujp 135 24 avirulent avirulent NN cord-291611-cfe8yujp 135 25 strains strain NNS cord-291611-cfe8yujp 135 26 while while IN cord-291611-cfe8yujp 135 27 the the DT cord-291611-cfe8yujp 135 28 sequence sequence NN cord-291611-cfe8yujp 135 29 KRRSVR KRRSVR NNP cord-291611-cfe8yujp 135 30 occurred occur VBD cord-291611-cfe8yujp 135 31 in in IN cord-291611-cfe8yujp 135 32 the the DT cord-291611-cfe8yujp 135 33 wild wild JJ cord-291611-cfe8yujp 135 34 - - HYPH cord-291611-cfe8yujp 135 35 type type NN cord-291611-cfe8yujp 135 36 BCV BCV NNP cord-291611-cfe8yujp 135 37 - - HYPH cord-291611-cfe8yujp 135 38 F15 F15 NNP cord-291611-cfe8yujp 135 39 ( ( -LRB- cord-291611-cfe8yujp 135 40 Fig Fig NNP cord-291611-cfe8yujp 135 41 . . . cord-291611-cfe8yujp 135 42 3 3 CD cord-291611-cfe8yujp 135 43 ) ) -RRB- cord-291611-cfe8yujp 135 44 . . . cord-291611-cfe8yujp 136 1 The the DT cord-291611-cfe8yujp 136 2 cleavage cleavage NN cord-291611-cfe8yujp 136 3 site site NN cord-291611-cfe8yujp 136 4 of of IN cord-291611-cfe8yujp 136 5 BCV BCV NNP cord-291611-cfe8yujp 136 6 S S NNP cord-291611-cfe8yujp 136 7 protein protein NN cord-291611-cfe8yujp 136 8 is be VBZ cord-291611-cfe8yujp 136 9 located locate VBN cord-291611-cfe8yujp 136 10 in in IN cord-291611-cfe8yujp 136 11 a a DT cord-291611-cfe8yujp 136 12 hydrophilic hydrophilic JJ cord-291611-cfe8yujp 136 13 area area NN cord-291611-cfe8yujp 136 14 , , , cord-291611-cfe8yujp 136 15 in in IN cord-291611-cfe8yujp 136 16 contrast contrast NN cord-291611-cfe8yujp 136 17 to to IN cord-291611-cfe8yujp 136 18 paramyxoviruses paramyxoviruses NNP cord-291611-cfe8yujp 136 19 and and CC cord-291611-cfe8yujp 136 20 myxoviruses myxoviruses NNP cord-291611-cfe8yujp 136 21 , , , cord-291611-cfe8yujp 136 22 in in IN cord-291611-cfe8yujp 136 23 which which WDT cord-291611-cfe8yujp 136 24 the the DT cord-291611-cfe8yujp 136 25 cleavage cleavage NN cord-291611-cfe8yujp 136 26 site site NN cord-291611-cfe8yujp 136 27 is be VBZ cord-291611-cfe8yujp 136 28 located locate VBN cord-291611-cfe8yujp 136 29 in in IN cord-291611-cfe8yujp 136 30 hydrophobic hydrophobic JJ cord-291611-cfe8yujp 136 31 domains domain NNS cord-291611-cfe8yujp 136 32 ( ( -LRB- cord-291611-cfe8yujp 136 33 20 20 CD cord-291611-cfe8yujp 136 34 - - SYM cord-291611-cfe8yujp 136 35 30 30 CD cord-291611-cfe8yujp 136 36 aa aa NN cord-291611-cfe8yujp 136 37 ) ) -RRB- cord-291611-cfe8yujp 136 38 of of IN cord-291611-cfe8yujp 136 39 the the DT cord-291611-cfe8yujp 136 40 fusion fusion NN cord-291611-cfe8yujp 136 41 proteins protein NNS cord-291611-cfe8yujp 136 42 ( ( -LRB- cord-291611-cfe8yujp 136 43 19 19 CD cord-291611-cfe8yujp 136 44 , , , cord-291611-cfe8yujp 136 45 20 20 CD cord-291611-cfe8yujp 136 46 ) ) -RRB- cord-291611-cfe8yujp 136 47 . . . cord-291611-cfe8yujp 137 1 These these DT cord-291611-cfe8yujp 137 2 observations observation NNS cord-291611-cfe8yujp 137 3 imply imply VBP cord-291611-cfe8yujp 137 4 that that IN cord-291611-cfe8yujp 137 5 there there EX cord-291611-cfe8yujp 137 6 might may MD cord-291611-cfe8yujp 137 7 be be VB cord-291611-cfe8yujp 137 8 a a DT cord-291611-cfe8yujp 137 9 different different JJ cord-291611-cfe8yujp 137 10 mechanism mechanism NN cord-291611-cfe8yujp 137 11 involved involve VBN cord-291611-cfe8yujp 137 12 in in IN cord-291611-cfe8yujp 137 13 BCV BCV NNP cord-291611-cfe8yujp 137 14 - - HYPH cord-291611-cfe8yujp 137 15 induced induce VBN cord-291611-cfe8yujp 137 16 cell cell NN cord-291611-cfe8yujp 137 17 fusion fusion NN cord-291611-cfe8yujp 137 18 . . . cord-291611-cfe8yujp 138 1 Parker Parker NNP cord-291611-cfe8yujp 138 2 et et NNP cord-291611-cfe8yujp 138 3 al al NNP cord-291611-cfe8yujp 138 4 . . . cord-291611-cfe8yujp 139 1 ( ( -LRB- cord-291611-cfe8yujp 139 2 14 14 CD cord-291611-cfe8yujp 139 3 ) ) -RRB- cord-291611-cfe8yujp 139 4 indicated indicate VBD cord-291611-cfe8yujp 139 5 that that IN cord-291611-cfe8yujp 139 6 a a DT cord-291611-cfe8yujp 139 7 sequence sequence NN cord-291611-cfe8yujp 139 8 VLGCLGSAC vlgclgsac NN cord-291611-cfe8yujp 139 9 ( ( -LRB- cord-291611-cfe8yujp 139 10 905 905 CD cord-291611-cfe8yujp 139 11 - - SYM cord-291611-cfe8yujp 139 12 913 913 CD cord-291611-cfe8yujp 139 13 ) ) -RRB- cord-291611-cfe8yujp 139 14 on on IN cord-291611-cfe8yujp 139 15 the the DT cord-291611-cfe8yujp 139 16 S S NNP cord-291611-cfe8yujp 139 17 protein protein NN cord-291611-cfe8yujp 139 18 of of IN cord-291611-cfe8yujp 139 19 BCV BCV NNP cord-291611-cfe8yujp 139 20 - - HYPH cord-291611-cfe8yujp 139 21 Quebec Quebec NNP cord-291611-cfe8yujp 139 22 may may MD cord-291611-cfe8yujp 139 23 constitute constitute VB cord-291611-cfe8yujp 139 24 a a DT cord-291611-cfe8yujp 139 25 portion portion NN cord-291611-cfe8yujp 139 26 of of IN cord-291611-cfe8yujp 139 27 the the DT cord-291611-cfe8yujp 139 28 fusogenic fusogenic JJ cord-291611-cfe8yujp 139 29 domain domain NN cord-291611-cfe8yujp 139 30 within within IN cord-291611-cfe8yujp 139 31 the the DT cord-291611-cfe8yujp 139 32 BCV BCV NNP cord-291611-cfe8yujp 139 33 S2 S2 NNP cord-291611-cfe8yujp 139 34 subunit subunit NN cord-291611-cfe8yujp 139 35 . . . cord-291611-cfe8yujp 140 1 This this DT cord-291611-cfe8yujp 140 2 stretch stretch NN cord-291611-cfe8yujp 140 3 is be VBZ cord-291611-cfe8yujp 140 4 similar similar JJ cord-291611-cfe8yujp 140 5 to to IN cord-291611-cfe8yujp 140 6 the the DT cord-291611-cfe8yujp 140 7 sequence sequence NN cord-291611-cfe8yujp 140 8 LLGCIGSTC LLGCIGSTC NNP cord-291611-cfe8yujp 140 9 of of IN cord-291611-cfe8yujp 140 10 MHV MHV NNP cord-291611-cfe8yujp 140 11 - - HYPH cord-291611-cfe8yujp 140 12 A59 A59 NNP cord-291611-cfe8yujp 140 13 , , , cord-291611-cfe8yujp 140 14 which which WDT cord-291611-cfe8yujp 140 15 contains contain VBZ cord-291611-cfe8yujp 140 16 a a DT cord-291611-cfe8yujp 140 17 neutralizing neutralize VBG cord-291611-cfe8yujp 140 18 epitope epitope NN cord-291611-cfe8yujp 140 19 ( ( -LRB- cord-291611-cfe8yujp 140 20 25 25 CD cord-291611-cfe8yujp 140 21 ) ) -RRB- cord-291611-cfe8yujp 140 22 . . . cord-291611-cfe8yujp 141 1 By by IN cord-291611-cfe8yujp 141 2 comparing compare VBG cord-291611-cfe8yujp 141 3 with with IN cord-291611-cfe8yujp 141 4 paramyxovirus paramyxovirus NN cord-291611-cfe8yujp 141 5 and and CC cord-291611-cfe8yujp 141 6 retrovirus retrovirus NN cord-291611-cfe8yujp 141 7 , , , cord-291611-cfe8yujp 141 8 Chambers Chambers NNP cord-291611-cfe8yujp 141 9 et et NNP cord-291611-cfe8yujp 141 10 a/. a/. NNP cord-291611-cfe8yujp 141 11 ( ( -LRB- cord-291611-cfe8yujp 141 12 26 26 CD cord-291611-cfe8yujp 141 13 ) ) -RRB- cord-291611-cfe8yujp 141 14 proposed propose VBD cord-291611-cfe8yujp 141 15 that that IN cord-291611-cfe8yujp 141 16 the the DT cord-291611-cfe8yujp 141 17 hydrophobic hydrophobic JJ cord-291611-cfe8yujp 141 18 region region NN cord-291611-cfe8yujp 141 19 adjacent adjacent JJ cord-291611-cfe8yujp 141 20 to to IN cord-291611-cfe8yujp 141 21 heptad heptad NN cord-291611-cfe8yujp 141 22 repeat repeat NN cord-291611-cfe8yujp 141 23 sequences sequence NNS cord-291611-cfe8yujp 141 24 may may MD cord-291611-cfe8yujp 141 25 be be VB cord-291611-cfe8yujp 141 26 the the DT cord-291611-cfe8yujp 141 27 potentially potentially RB cord-291611-cfe8yujp 141 28 fusion fusion NN cord-291611-cfe8yujp 141 29 - - HYPH cord-291611-cfe8yujp 141 30 related relate VBN cord-291611-cfe8yujp 141 31 domain domain NN cord-291611-cfe8yujp 141 32 in in IN cord-291611-cfe8yujp 141 33 the the DT cord-291611-cfe8yujp 141 34 S S NNP cord-291611-cfe8yujp 141 35 proteins protein NNS cord-291611-cfe8yujp 141 36 of of IN cord-291611-cfe8yujp 141 37 mouse mouse NN cord-291611-cfe8yujp 141 38 hepatitis hepatitis NNP cord-291611-cfe8yujp 141 39 virus virus NN cord-291611-cfe8yujp 141 40 , , , cord-291611-cfe8yujp 141 41 infectious infectious JJ cord-291611-cfe8yujp 141 42 bronchitis bronchitis NN cord-291611-cfe8yujp 141 43 virus virus NN cord-291611-cfe8yujp 141 44 , , , cord-291611-cfe8yujp 141 45 and and CC cord-291611-cfe8yujp 141 46 transmissible transmissible JJ cord-291611-cfe8yujp 141 47 gastroenteritis gastroenteritis NN cord-291611-cfe8yujp 141 48 virus virus NN cord-291611-cfe8yujp 141 49 . . . cord-291611-cfe8yujp 142 1 After after IN cord-291611-cfe8yujp 142 2 multiple multiple JJ cord-291611-cfe8yujp 142 3 alignments alignment NNS cord-291611-cfe8yujp 142 4 it -PRON- PRP cord-291611-cfe8yujp 142 5 is be VBZ cord-291611-cfe8yujp 142 6 found find VBN cord-291611-cfe8yujp 142 7 that that IN cord-291611-cfe8yujp 142 8 the the DT cord-291611-cfe8yujp 142 9 heptad heptad NN cord-291611-cfe8yujp 142 10 repeat repeat NN cord-291611-cfe8yujp 142 11 sequences sequence NNS cord-291611-cfe8yujp 142 12 are be VBP cord-291611-cfe8yujp 142 13 located locate VBN cord-291611-cfe8yujp 142 14 in in IN cord-291611-cfe8yujp 142 15 the the DT cord-291611-cfe8yujp 142 16 S2 s2 NN cord-291611-cfe8yujp 142 17 at at IN cord-291611-cfe8yujp 142 18 positions position NNS cord-291611-cfe8yujp 142 19 999-l 999-l CD cord-291611-cfe8yujp 142 20 038 038 CD cord-291611-cfe8yujp 142 21 which which WDT cord-291611-cfe8yujp 142 22 are be VBP cord-291611-cfe8yujp 142 23 conserved conserve VBN cord-291611-cfe8yujp 142 24 in in IN cord-291611-cfe8yujp 142 25 all all DT cord-291611-cfe8yujp 142 26 strains strain NNS cord-291611-cfe8yujp 142 27 , , , cord-291611-cfe8yujp 142 28 and and CC cord-291611-cfe8yujp 142 29 the the DT cord-291611-cfe8yujp 142 30 hydrophobic hydrophobic JJ cord-291611-cfe8yujp 142 31 regions region NNS cord-291611-cfe8yujp 142 32 are be VBP cord-291611-cfe8yujp 142 33 located locate VBN cord-291611-cfe8yujp 142 34 at at IN cord-291611-cfe8yujp 142 35 961 961 CD cord-291611-cfe8yujp 142 36 - - HYPH cord-291611-cfe8yujp 142 37 998 998 CD cord-291611-cfe8yujp 142 38 ( ( -LRB- cord-291611-cfe8yujp 142 39 see see VB cord-291611-cfe8yujp 142 40 Fig Fig NNP cord-291611-cfe8yujp 142 41 . . . cord-291611-cfe8yujp 142 42 3 3 CD cord-291611-cfe8yujp 142 43 ) ) -RRB- cord-291611-cfe8yujp 142 44 . . . cord-291611-cfe8yujp 143 1 However however RB cord-291611-cfe8yujp 143 2 , , , cord-291611-cfe8yujp 143 3 a a DT cord-291611-cfe8yujp 143 4 direct direct JJ cord-291611-cfe8yujp 143 5 involvement involvement NN cord-291611-cfe8yujp 143 6 of of IN cord-291611-cfe8yujp 143 7 these these DT cord-291611-cfe8yujp 143 8 sequences sequence NNS cord-291611-cfe8yujp 143 9 in in IN cord-291611-cfe8yujp 143 10 BCV BCV NNP cord-291611-cfe8yujp 143 11 fusion fusion NN cord-291611-cfe8yujp 143 12 has have VBZ cord-291611-cfe8yujp 143 13 yet yet RB cord-291611-cfe8yujp 143 14 to to TO cord-291611-cfe8yujp 143 15 be be VB cord-291611-cfe8yujp 143 16 demonstrated demonstrate VBN cord-291611-cfe8yujp 143 17 . . . cord-291611-cfe8yujp 144 1 Similarly similarly RB cord-291611-cfe8yujp 144 2 , , , cord-291611-cfe8yujp 144 3 the the DT cord-291611-cfe8yujp 144 4 El El NNP cord-291611-cfe8yujp 144 5 protein protein NN cord-291611-cfe8yujp 144 6 of of IN cord-291611-cfe8yujp 144 7 Semliki Semliki NNP cord-291611-cfe8yujp 144 8 Forest Forest NNP cord-291611-cfe8yujp 144 9 virus virus NN cord-291611-cfe8yujp 144 10 ( ( -LRB- cord-291611-cfe8yujp 144 11 SFV SFV NNP cord-291611-cfe8yujp 144 12 ) ) -RRB- cord-291611-cfe8yujp 144 13 does do VBZ cord-291611-cfe8yujp 144 14 not not RB cord-291611-cfe8yujp 144 15 contain contain VB cord-291611-cfe8yujp 144 16 a a DT cord-291611-cfe8yujp 144 17 hydrophobic hydrophobic JJ cord-291611-cfe8yujp 144 18 N n CD cord-291611-cfe8yujp 144 19 - - HYPH cord-291611-cfe8yujp 144 20 terminal terminal NN cord-291611-cfe8yujp 144 21 region region NN cord-291611-cfe8yujp 144 22 . . . cord-291611-cfe8yujp 145 1 It -PRON- PRP cord-291611-cfe8yujp 145 2 has have VBZ cord-291611-cfe8yujp 145 3 been be VBN cord-291611-cfe8yujp 145 4 proposed propose VBN cord-291611-cfe8yujp 145 5 , , , cord-291611-cfe8yujp 145 6 however however RB cord-291611-cfe8yujp 145 7 , , , cord-291611-cfe8yujp 145 8 that that IN cord-291611-cfe8yujp 145 9 in in IN cord-291611-cfe8yujp 145 10 El El NNP cord-291611-cfe8yujp 145 11 an an DT cord-291611-cfe8yujp 145 12 internal internal JJ cord-291611-cfe8yujp 145 13 uncharged uncharged JJ cord-291611-cfe8yujp 145 14 stretch stretch NN cord-291611-cfe8yujp 145 15 of of IN cord-291611-cfe8yujp 145 16 17 17 CD cord-291611-cfe8yujp 145 17 residues residue NNS cord-291611-cfe8yujp 145 18 , , , cord-291611-cfe8yujp 145 19 located locate VBN cord-291611-cfe8yujp 145 20 about about RB cord-291611-cfe8yujp 145 21 80 80 CD cord-291611-cfe8yujp 145 22 aa aa NN cord-291611-cfe8yujp 145 23 from from IN cord-291611-cfe8yujp 145 24 the the DT cord-291611-cfe8yujp 145 25 N N NNP cord-291611-cfe8yujp 145 26 - - HYPH cord-291611-cfe8yujp 145 27 terminal terminal NN cord-291611-cfe8yujp 145 28 , , , cord-291611-cfe8yujp 145 29 might may MD cord-291611-cfe8yujp 145 30 act act VB cord-291611-cfe8yujp 145 31 as as IN cord-291611-cfe8yujp 145 32 the the DT cord-291611-cfe8yujp 145 33 putative putative JJ cord-291611-cfe8yujp 145 34 fusogenic fusogenic JJ cord-291611-cfe8yujp 145 35 domain domain NN cord-291611-cfe8yujp 145 36 ( ( -LRB- cord-291611-cfe8yujp 145 37 27 27 CD cord-291611-cfe8yujp 145 38 ) ) -RRB- cord-291611-cfe8yujp 145 39 . . . cord-291611-cfe8yujp 146 1 Interestingly interestingly RB cord-291611-cfe8yujp 146 2 , , , cord-291611-cfe8yujp 146 3 we -PRON- PRP cord-291611-cfe8yujp 146 4 found find VBD cord-291611-cfe8yujp 146 5 three three CD cord-291611-cfe8yujp 146 6 patterns pattern NNS cord-291611-cfe8yujp 146 7 of of IN cord-291611-cfe8yujp 146 8 amino amino NN cord-291611-cfe8yujp 146 9 acid acid NN cord-291611-cfe8yujp 146 10 substitution substitution NN cord-291611-cfe8yujp 146 11 in in IN cord-291611-cfe8yujp 146 12 the the DT cord-291611-cfe8yujp 146 13 putative putative JJ cord-291611-cfe8yujp 146 14 fusogenic fusogenic JJ cord-291611-cfe8yujp 146 15 domain domain NN cord-291611-cfe8yujp 146 16 : : : cord-291611-cfe8yujp 147 1 at at IN cord-291611-cfe8yujp 147 2 position position NN cord-291611-cfe8yujp 147 3 971 971 CD cord-291611-cfe8yujp 147 4 , , , cord-291611-cfe8yujp 147 5 V v NN cord-291611-cfe8yujp 147 6 in in IN cord-291611-cfe8yujp 147 7 the the DT cord-291611-cfe8yujp 147 8 avirulent avirulent NN cord-291611-cfe8yujp 147 9 strains strain NNS cord-291611-cfe8yujp 147 10 was be VBD cord-291611-cfe8yujp 147 11 substituted substitute VBN cord-291611-cfe8yujp 147 12 by by IN cord-291611-cfe8yujp 147 13 E E NNS cord-291611-cfe8yujp 147 14 in in IN cord-291611-cfe8yujp 147 15 the the DT cord-291611-cfe8yujp 147 16 virulent virulent JJ cord-291611-cfe8yujp 147 17 strains strain NNS cord-291611-cfe8yujp 147 18 ; ; : cord-291611-cfe8yujp 147 19 W w NN cord-291611-cfe8yujp 147 20 ( ( -LRB- cord-291611-cfe8yujp 147 21 898 898 CD cord-291611-cfe8yujp 147 22 ) ) -RRB- cord-291611-cfe8yujp 147 23 and and CC cord-291611-cfe8yujp 147 24 A a NN cord-291611-cfe8yujp 147 25 ( ( -LRB- cord-291611-cfe8yujp 147 26 993 993 CD cord-291611-cfe8yujp 147 27 ) ) -RRB- cord-291611-cfe8yujp 147 28 were be VBD cord-291611-cfe8yujp 147 29 substituted substitute VBN cord-291611-cfe8yujp 147 30 by by IN cord-291611-cfe8yujp 147 31 I -PRON- PRP cord-291611-cfe8yujp 147 32 and and CC cord-291611-cfe8yujp 147 33 V v NN cord-291611-cfe8yujp 147 34 in in IN cord-291611-cfe8yujp 147 35 BCV BCV NNP cord-291611-cfe8yujp 147 36 - - HYPH cord-291611-cfe8yujp 147 37 Mebus Mebus NNP cord-291611-cfe8yujp 147 38 and and CC cord-291611-cfe8yujp 147 39 BCV BCV NNP cord-291611-cfe8yujp 147 40 - - HYPH cord-291611-cfe8yujp 147 41 Quebec Quebec NNP cord-291611-cfe8yujp 147 42 , , , cord-291611-cfe8yujp 147 43 respectively respectively RB cord-291611-cfe8yujp 147 44 . . . cord-291611-cfe8yujp 148 1 It -PRON- PRP cord-291611-cfe8yujp 148 2 is be VBZ cord-291611-cfe8yujp 148 3 unclear unclear JJ cord-291611-cfe8yujp 148 4 , , , cord-291611-cfe8yujp 148 5 however however RB cord-291611-cfe8yujp 148 6 , , , cord-291611-cfe8yujp 148 7 whether whether IN cord-291611-cfe8yujp 148 8 these these DT cord-291611-cfe8yujp 148 9 amino amino NN cord-291611-cfe8yujp 148 10 acid acid NN cord-291611-cfe8yujp 148 11 changes change NNS cord-291611-cfe8yujp 148 12 in in IN cord-291611-cfe8yujp 148 13 the the DT cord-291611-cfe8yujp 148 14 predicted predict VBN cord-291611-cfe8yujp 148 15 cleavage cleavage NN cord-291611-cfe8yujp 148 16 sites site NNS cord-291611-cfe8yujp 148 17 and and CC cord-291611-cfe8yujp 148 18 the the DT cord-291611-cfe8yujp 148 19 putative putative JJ cord-291611-cfe8yujp 148 20 fusogenic fusogenic JJ cord-291611-cfe8yujp 148 21 domains domain NNS cord-291611-cfe8yujp 148 22 reflect reflect VBP cord-291611-cfe8yujp 148 23 any any DT cord-291611-cfe8yujp 148 24 functional functional JJ cord-291611-cfe8yujp 148 25 differences difference NNS cord-291611-cfe8yujp 148 26 , , , cord-291611-cfe8yujp 148 27 such such JJ cord-291611-cfe8yujp 148 28 as as IN cord-291611-cfe8yujp 148 29 fusion fusion NN cord-291611-cfe8yujp 148 30 activities activity NNS cord-291611-cfe8yujp 148 31 among among IN cord-291611-cfe8yujp 148 32 different different JJ cord-291611-cfe8yujp 148 33 BCV BCV NNP cord-291611-cfe8yujp 148 34 strains strain NNS cord-291611-cfe8yujp 148 35 . . . cord-291611-cfe8yujp 149 1 Proteins Proteins NNP cord-291611-cfe8yujp 149 2 Enterites Enterites NNPS cord-291611-cfe8yujp 150 1 Virales Virales NNP cord-291611-cfe8yujp 150 2 / / SYM cord-291611-cfe8yujp 150 3 Viral Viral NNP cord-291611-cfe8yujp 150 4 Enteritis Enteritis NNP cord-291611-cfe8yujp 150 5 in in IN cord-291611-cfe8yujp 150 6 Human Human NNP cord-291611-cfe8yujp 150 7 and and CC cord-291611-cfe8yujp 150 8 Animals Animals NNPS cord-291611-cfe8yujp 151 1 This this DT cord-291611-cfe8yujp 151 2 work work NN cord-291611-cfe8yujp 151 3 was be VBD cord-291611-cfe8yujp 151 4 supported support VBN cord-291611-cfe8yujp 151 5 by by IN cord-291611-cfe8yujp 151 6 Grants Grants NNPS cord-291611-cfe8yujp 151 7 86-CRSR-2 86-crsr-2 CD cord-291611-cfe8yujp 151 8 - - SYM cord-291611-cfe8yujp 151 9 2871 2871 CD cord-291611-cfe8yujp 151 10 and and CC cord-291611-cfe8yujp 151 11 89 89 CD cord-291611-cfe8yujp 151 12 . . . cord-291611-cfe8yujp 152 1 34116 34116 CD cord-291611-cfe8yujp 152 2 - - SYM cord-291611-cfe8yujp 152 3 4675 4675 CD cord-291611-cfe8yujp 152 4 from from IN cord-291611-cfe8yujp 152 5 the the DT cord-291611-cfe8yujp 152 6 United United NNP cord-291611-cfe8yujp 152 7 States States NNP cord-291611-cfe8yujp 152 8 Department Department NNP cord-291611-cfe8yujp 152 9 of of IN cord-291611-cfe8yujp 152 10 Agriculture Agriculture NNP cord-291611-cfe8yujp 152 11 , , , cord-291611-cfe8yujp 152 12 and and CC cord-291611-cfe8yujp 152 13 Louisiana Louisiana NNP cord-291611-cfe8yujp 152 14 Education Education NNP cord-291611-cfe8yujp 152 15 Quality Quality NNP cord-291611-cfe8yujp 152 16 Support Support NNP cord-291611-cfe8yujp 152 17 Fund Fund NNP cord-291611-cfe8yujp 152 18 of of IN cord-291611-cfe8yujp 152 19 the the DT cord-291611-cfe8yujp 152 20 Board Board NNP cord-291611-cfe8yujp 152 21 of of IN cord-291611-cfe8yujp 152 22 Regents Regents NNPS cord-291611-cfe8yujp 152 23 , , , cord-291611-cfe8yujp 152 24 State State NNP cord-291611-cfe8yujp 152 25 of of IN cord-291611-cfe8yujp 152 26 Louisiana Louisiana NNP cord-291611-cfe8yujp 152 27 , , , cord-291611-cfe8yujp 152 28 to to IN cord-291611-cfe8yujp 152 29 J.S. J.S. NNP cord-291611-cfe8yujp 152 30 and and CC cord-291611-cfe8yujp 152 31 K.G.K K.G.K NNP cord-291611-cfe8yujp 152 32 .. .. . cord-291611-cfe8yujp 153 1 and and CC cord-291611-cfe8yujp 153 2 Grant Grant NNP cord-291611-cfe8yujp 153 3 Al87886 Al87886 NNP cord-291611-cfe8yujp 154 1 to to IN cord-291611-cfe8yujp 154 2 K.G.K. K.G.K. NNP cord-291611-cfe8yujp 155 1 We -PRON- PRP cord-291611-cfe8yujp 155 2 thank thank VBP cord-291611-cfe8yujp 155 3 Mamie Mamie NNP cord-291611-cfe8yujp 155 4 Burrell Burrell NNP cord-291611-cfe8yujp 155 5 and and CC cord-291611-cfe8yujp 155 6 Li Li NNP cord-291611-cfe8yujp 155 7 - - HYPH cord-291611-cfe8yujp 155 8 Ju Ju NNP cord-291611-cfe8yujp 155 9 T. T. NNP cord-291611-cfe8yujp 155 10 Huang Huang NNP cord-291611-cfe8yujp 155 11 for for IN cord-291611-cfe8yujp 155 12 excellent excellent JJ cord-291611-cfe8yujp 155 13 technical technical JJ cord-291611-cfe8yujp 155 14 assistance assistance NN cord-291611-cfe8yujp 155 15 , , , cord-291611-cfe8yujp 155 16 Susan Susan NNP cord-291611-cfe8yujp 155 17 Newman Newman NNP cord-291611-cfe8yujp 155 18 for for IN cord-291611-cfe8yujp 155 19 computer computer NN cord-291611-cfe8yujp 155 20 assistance assistance NN cord-291611-cfe8yujp 155 21 , , , cord-291611-cfe8yujp 155 22 and and CC cord-291611-cfe8yujp 155 23 Dr. Dr. NNP cord-291611-cfe8yujp 155 24 B. B. NNP cord-291611-cfe8yujp 155 25 Kaltenboeck Kaltenboeck NNP cord-291611-cfe8yujp 155 26 for for IN cord-291611-cfe8yujp 155 27 valuable valuable JJ cord-291611-cfe8yujp 155 28 discussion discussion NN cord-291611-cfe8yujp 155 29 on on IN cord-291611-cfe8yujp 155 30 PCR PCR NNP cord-291611-cfe8yujp 155 31 technique technique NN cord-291611-cfe8yujp 155 32 . . .