id	sid	tid	token	lemma	pos
cord-308835-999kewdw	1	1	key	key	NN
cord-308835-999kewdw	1	2	:	:	:
cord-308835-999kewdw	2	1	cord-308835	cord-308835	NNP
cord-308835-999kewdw	2	2	-	-	HYPH
cord-308835-999kewdw	2	3	999kewdw	999kewdw	JJ
cord-308835-999kewdw	2	4	authors	author	NNS
cord-308835-999kewdw	2	5	:	:	:
cord-308835-999kewdw	2	6	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	2	7	,	,	,
cord-308835-999kewdw	2	8	Julian	Julian	NNP
cord-308835-999kewdw	2	9	L.	L.	NNP
cord-308835-999kewdw	2	10	;	;	:
cord-308835-999kewdw	2	11	Wilhelmsen	Wilhelmsen	NNP
cord-308835-999kewdw	2	12	,	,	,
cord-308835-999kewdw	2	13	Kirk	Kirk	NNP
cord-308835-999kewdw	2	14	C.	C.	NNP
cord-308835-999kewdw	2	15	;	;	:
cord-308835-999kewdw	2	16	Bond	Bond	NNP
cord-308835-999kewdw	2	17	,	,	,
cord-308835-999kewdw	2	18	Clifford	Clifford	NNP
cord-308835-999kewdw	2	19	W.	W.	NNP
cord-308835-999kewdw	2	20	title	title	NN
cord-308835-999kewdw	2	21	:	:	:
cord-308835-999kewdw	2	22	The	the	DT
cord-308835-999kewdw	2	23	virus	virus	NN
cord-308835-999kewdw	2	24	-	-	HYPH
cord-308835-999kewdw	2	25	specific	specific	JJ
cord-308835-999kewdw	2	26	intracellular	intracellular	JJ
cord-308835-999kewdw	2	27	RNA	RNA	NNP
cord-308835-999kewdw	2	28	species	specie	NNS
cord-308835-999kewdw	2	29	of	of	IN
cord-308835-999kewdw	2	30	two	two	CD
cord-308835-999kewdw	2	31	murine	murine	JJ
cord-308835-999kewdw	2	32	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	2	33	:	:	:
cord-308835-999kewdw	2	34	MHV	MHV	NNP
cord-308835-999kewdw	2	35	-	-	HYPH
cord-308835-999kewdw	2	36	A59	A59	NNP
cord-308835-999kewdw	2	37	and	and	CC
cord-308835-999kewdw	2	38	MHV	MHV	NNP
cord-308835-999kewdw	2	39	-	-	HYPH
cord-308835-999kewdw	2	40	JHM	JHM	NNP
cord-308835-999kewdw	2	41	date	date	NN
cord-308835-999kewdw	2	42	:	:	:
cord-308835-999kewdw	2	43	1981	1981	CD
cord-308835-999kewdw	2	44	-	-	SYM
cord-308835-999kewdw	2	45	10	10	CD
cord-308835-999kewdw	2	46	-	-	SYM
cord-308835-999kewdw	2	47	15	15	CD
cord-308835-999kewdw	2	48	journal	journal	NN
cord-308835-999kewdw	2	49	:	:	:
cord-308835-999kewdw	2	50	Virology	Virology	NNP
cord-308835-999kewdw	2	51	DOI	DOI	NNP
cord-308835-999kewdw	2	52	:	:	:
cord-308835-999kewdw	2	53	10.1016/0042	10.1016/0042	CD
cord-308835-999kewdw	2	54	-	-	HYPH
cord-308835-999kewdw	2	55	6822(81)90250	6822(81)90250	CD
cord-308835-999kewdw	2	56	-	-	HYPH
cord-308835-999kewdw	2	57	6	6	CD
cord-308835-999kewdw	2	58	sha	sha	NN
cord-308835-999kewdw	2	59	:	:	:
cord-308835-999kewdw	2	60	f66156b6a380cb402e6006102ab714fab10e9170	f66156b6a380cb402e6006102ab714fab10e9170	VB
cord-308835-999kewdw	3	1	doc_id	doc_id	CD
cord-308835-999kewdw	3	2	:	:	:
cord-308835-999kewdw	4	1	308835	308835	CD
cord-308835-999kewdw	4	2	cord_uid	cord_uid	NN
cord-308835-999kewdw	4	3	:	:	:
cord-308835-999kewdw	5	1	999kewdw	999kewdw	NNP
cord-308835-999kewdw	6	1	Abstract	Abstract	NNP
cord-308835-999kewdw	6	2	Seven	seven	CD
cord-308835-999kewdw	6	3	virus	virus	NN
cord-308835-999kewdw	6	4	-	-	HYPH
cord-308835-999kewdw	6	5	specific	specific	JJ
cord-308835-999kewdw	6	6	,	,	,
cord-308835-999kewdw	6	7	polyadenylated	polyadenylated	JJ
cord-308835-999kewdw	6	8	RNA	RNA	NNP
cord-308835-999kewdw	6	9	species	specie	NNS
cord-308835-999kewdw	6	10	have	have	VBP
cord-308835-999kewdw	6	11	been	be	VBN
cord-308835-999kewdw	6	12	identified	identify	VBN
cord-308835-999kewdw	6	13	in	in	IN
cord-308835-999kewdw	6	14	mouse	mouse	NN
cord-308835-999kewdw	6	15	cells	cell	NNS
cord-308835-999kewdw	6	16	infected	infect	VBN
cord-308835-999kewdw	6	17	with	with	IN
cord-308835-999kewdw	6	18	the	the	DT
cord-308835-999kewdw	6	19	murine	murine	JJ
cord-308835-999kewdw	6	20	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	6	21	MHV	MHV	NNP
cord-308835-999kewdw	6	22	-	-	HYPH
cord-308835-999kewdw	6	23	A59	A59	NNP
cord-308835-999kewdw	6	24	(	(	-LRB-
cord-308835-999kewdw	6	25	A59V	A59V	NNP
cord-308835-999kewdw	6	26	)	)	-RRB-
cord-308835-999kewdw	6	27	or	or	CC
cord-308835-999kewdw	6	28	MHV	MHV	NNP
cord-308835-999kewdw	6	29	-	-	HYPH
cord-308835-999kewdw	6	30	JHM	JHM	NNP
cord-308835-999kewdw	6	31	(	(	-LRB-
cord-308835-999kewdw	6	32	JHMV	JHMV	NNP
cord-308835-999kewdw	6	33	)	)	-RRB-
cord-308835-999kewdw	6	34	.	.	.
cord-308835-999kewdw	7	1	MHV	mhv	NN
cord-308835-999kewdw	7	2	-	-	HYPH
cord-308835-999kewdw	7	3	infected	infect	VBN
cord-308835-999kewdw	7	4	17CL·1	17cl·1	CD
cord-308835-999kewdw	7	5	cells	cell	NNS
cord-308835-999kewdw	7	6	were	be	VBD
cord-308835-999kewdw	7	7	labeled	label	VBN
cord-308835-999kewdw	7	8	with	with	IN
cord-308835-999kewdw	7	9	[	[	-LRB-
cord-308835-999kewdw	7	10	32P]orthophosphate	32p]orthophosphate	NN
cord-308835-999kewdw	7	11	in	in	IN
cord-308835-999kewdw	7	12	the	the	DT
cord-308835-999kewdw	7	13	presence	presence	NN
cord-308835-999kewdw	7	14	of	of	IN
cord-308835-999kewdw	7	15	actinomycin	actinomycin	NNS
cord-308835-999kewdw	7	16	D	D	NNP
cord-308835-999kewdw	7	17	and	and	CC
cord-308835-999kewdw	7	18	the	the	DT
cord-308835-999kewdw	7	19	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	7	20	RNA	RNA	NNP
cord-308835-999kewdw	7	21	was	be	VBD
cord-308835-999kewdw	7	22	extracted	extract	VBN
cord-308835-999kewdw	7	23	and	and	CC
cord-308835-999kewdw	7	24	analyzed	analyze	VBN
cord-308835-999kewdw	7	25	by	by	IN
cord-308835-999kewdw	7	26	agarose	agarose	JJ
cord-308835-999kewdw	7	27	gel	gel	NN
cord-308835-999kewdw	7	28	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	7	29	.	.	.
cord-308835-999kewdw	8	1	These	these	DT
cord-308835-999kewdw	8	2	RNA	RNA	NNP
cord-308835-999kewdw	8	3	species	specie	NNS
cord-308835-999kewdw	8	4	range	range	NN
cord-308835-999kewdw	8	5	in	in	IN
cord-308835-999kewdw	8	6	size	size	NN
cord-308835-999kewdw	8	7	from	from	IN
cord-308835-999kewdw	8	8	6.3	6.3	CD
cord-308835-999kewdw	8	9	×	×	CD
cord-308835-999kewdw	8	10	105	105	CD
cord-308835-999kewdw	8	11	to	to	IN
cord-308835-999kewdw	8	12	6.1	6.1	CD
cord-308835-999kewdw	8	13	×	×	NN
cord-308835-999kewdw	8	14	106	106	CD
cord-308835-999kewdw	8	15	daltons	dalton	NNS
cord-308835-999kewdw	8	16	.	.	.
cord-308835-999kewdw	9	1	The	the	DT
cord-308835-999kewdw	9	2	A59V	a59v	NN
cord-308835-999kewdw	9	3	and	and	CC
cord-308835-999kewdw	9	4	JHMV	JHMV	NNP
cord-308835-999kewdw	9	5	-	-	HYPH
cord-308835-999kewdw	9	6	specific	specific	JJ
cord-308835-999kewdw	9	7	RNAs	rna	NNS
cord-308835-999kewdw	9	8	have	have	VBP
cord-308835-999kewdw	9	9	identical	identical	JJ
cord-308835-999kewdw	9	10	molecular	molecular	JJ
cord-308835-999kewdw	9	11	weights	weight	NNS
cord-308835-999kewdw	9	12	and	and	CC
cord-308835-999kewdw	9	13	comigrate	comigrate	NN
cord-308835-999kewdw	9	14	in	in	IN
cord-308835-999kewdw	9	15	agarose	agarose	JJ
cord-308835-999kewdw	9	16	gels	gel	NNS
cord-308835-999kewdw	9	17	.	.	.
cord-308835-999kewdw	10	1	The	the	DT
cord-308835-999kewdw	10	2	largest	large	JJS
cord-308835-999kewdw	10	3	intracellular	intracellular	JJ
cord-308835-999kewdw	10	4	RNA	RNA	NNP
cord-308835-999kewdw	10	5	species	specie	NNS
cord-308835-999kewdw	10	6	is	be	VBZ
cord-308835-999kewdw	10	7	identical	identical	JJ
cord-308835-999kewdw	10	8	to	to	IN
cord-308835-999kewdw	10	9	RNA	RNA	NNP
cord-308835-999kewdw	10	10	isolated	isolate	VBN
cord-308835-999kewdw	10	11	from	from	IN
cord-308835-999kewdw	10	12	purified	purify	VBN
cord-308835-999kewdw	10	13	virions	virion	NNS
cord-308835-999kewdw	10	14	,	,	,
cord-308835-999kewdw	10	15	as	as	IN
cord-308835-999kewdw	10	16	determined	determine	VBN
cord-308835-999kewdw	10	17	by	by	IN
cord-308835-999kewdw	10	18	agarose	agarose	JJ
cord-308835-999kewdw	10	19	gel	gel	NN
cord-308835-999kewdw	10	20	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	10	21	and	and	CC
cord-308835-999kewdw	10	22	oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	10	23	fingerprint	fingerprint	NN
cord-308835-999kewdw	10	24	studies	study	NNS
cord-308835-999kewdw	10	25	of	of	IN
cord-308835-999kewdw	10	26	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	10	27	T1	T1	NNP
cord-308835-999kewdw	10	28	digests	digest	NNS
cord-308835-999kewdw	10	29	.	.	.
cord-308835-999kewdw	11	1	Oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	11	2	fingerprints	fingerprint	NNS
cord-308835-999kewdw	11	3	of	of	IN
cord-308835-999kewdw	11	4	the	the	DT
cord-308835-999kewdw	11	5	six	six	CD
cord-308835-999kewdw	11	6	subgenomic	subgenomic	JJ
cord-308835-999kewdw	11	7	RNAS	RNAS	NNP
cord-308835-999kewdw	11	8	show	show	VBP
cord-308835-999kewdw	11	9	that	that	IN
cord-308835-999kewdw	11	10	the	the	DT
cord-308835-999kewdw	11	11	sequences	sequence	NNS
cord-308835-999kewdw	11	12	they	-PRON-	PRP
cord-308835-999kewdw	11	13	contain	contain	VBP
cord-308835-999kewdw	11	14	are	be	VBP
cord-308835-999kewdw	11	15	present	present	JJ
cord-308835-999kewdw	11	16	in	in	IN
cord-308835-999kewdw	11	17	virion	virion	NN
cord-308835-999kewdw	11	18	RNA	RNA	NNP
cord-308835-999kewdw	11	19	,	,	,
cord-308835-999kewdw	11	20	confirming	confirm	VBG
cord-308835-999kewdw	11	21	their	-PRON-	PRP$
cord-308835-999kewdw	11	22	virus	virus	NN
cord-308835-999kewdw	11	23	-	-	HYPH
cord-308835-999kewdw	11	24	specific	specific	JJ
cord-308835-999kewdw	11	25	nature	nature	NN
cord-308835-999kewdw	11	26	.	.	.
cord-308835-999kewdw	12	1	The	the	DT
cord-308835-999kewdw	12	2	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	12	3	studies	study	NNS
cord-308835-999kewdw	12	4	also	also	RB
cord-308835-999kewdw	12	5	demonstrate	demonstrate	VBP
cord-308835-999kewdw	12	6	that	that	IN
cord-308835-999kewdw	12	7	the	the	DT
cord-308835-999kewdw	12	8	six	six	CD
cord-308835-999kewdw	12	9	subgenomic	subgenomic	NNP
cord-308835-999kewdw	12	10	RNA	RNA	NNP
cord-308835-999kewdw	12	11	species	specie	NNS
cord-308835-999kewdw	12	12	make	make	VBP
cord-308835-999kewdw	12	13	up	up	RP
cord-308835-999kewdw	12	14	a	a	DT
cord-308835-999kewdw	12	15	nested	nest	VBN
cord-308835-999kewdw	12	16	set	set	NN
cord-308835-999kewdw	12	17	.	.	.
cord-308835-999kewdw	13	1	The	the	DT
cord-308835-999kewdw	13	2	sequences	sequence	NNS
cord-308835-999kewdw	13	3	present	present	JJ
cord-308835-999kewdw	13	4	in	in	IN
cord-308835-999kewdw	13	5	each	each	DT
cord-308835-999kewdw	13	6	RNA	RNA	NNP
cord-308835-999kewdw	13	7	species	specie	NNS
cord-308835-999kewdw	13	8	are	be	VBP
cord-308835-999kewdw	13	9	also	also	RB
cord-308835-999kewdw	13	10	present	present	JJ
cord-308835-999kewdw	13	11	in	in	IN
cord-308835-999kewdw	13	12	all	all	DT
cord-308835-999kewdw	13	13	larger	large	JJR
cord-308835-999kewdw	13	14	RNA	RNA	NNP
cord-308835-999kewdw	13	15	species	specie	NNS
cord-308835-999kewdw	13	16	.	.	.
cord-308835-999kewdw	14	1	These	these	DT
cord-308835-999kewdw	14	2	larger	large	JJR
cord-308835-999kewdw	14	3	RNAs	rna	NNS
cord-308835-999kewdw	14	4	also	also	RB
cord-308835-999kewdw	14	5	contain	contain	VBP
cord-308835-999kewdw	14	6	additional	additional	JJ
cord-308835-999kewdw	14	7	sequences	sequence	NNS
cord-308835-999kewdw	14	8	consistent	consistent	JJ
cord-308835-999kewdw	14	9	with	with	IN
cord-308835-999kewdw	14	10	their	-PRON-	PRP$
cord-308835-999kewdw	14	11	greater	great	JJR
cord-308835-999kewdw	14	12	size	size	NN
cord-308835-999kewdw	14	13	.	.	.
cord-308835-999kewdw	15	1	The	the	DT
cord-308835-999kewdw	15	2	subgenomic	subgenomic	JJ
cord-308835-999kewdw	15	3	RNAs	rna	NNS
cord-308835-999kewdw	15	4	fulfull	fulfull	VBP
cord-308835-999kewdw	15	5	many	many	JJ
cord-308835-999kewdw	15	6	of	of	IN
cord-308835-999kewdw	15	7	the	the	DT
cord-308835-999kewdw	15	8	criteria	criterion	NNS
cord-308835-999kewdw	15	9	for	for	IN
cord-308835-999kewdw	15	10	mRNAs	mrnas	CD
cord-308835-999kewdw	15	11	.	.	.
cord-308835-999kewdw	16	1	Possible	possible	JJ
cord-308835-999kewdw	16	2	mechanisms	mechanism	NNS
cord-308835-999kewdw	16	3	for	for	IN
cord-308835-999kewdw	16	4	generating	generate	VBG
cord-308835-999kewdw	16	5	these	these	DT
cord-308835-999kewdw	16	6	RNAs	rna	NNS
cord-308835-999kewdw	16	7	are	be	VBP
cord-308835-999kewdw	16	8	discussed	discuss	VBN
cord-308835-999kewdw	16	9	.	.	.
cord-308835-999kewdw	17	1	Coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	17	2	are	be	VBP
cord-308835-999kewdw	17	3	widespread	widespread	JJ
cord-308835-999kewdw	17	4	in	in	IN
cord-308835-999kewdw	17	5	nature	nature	NN
cord-308835-999kewdw	17	6	and	and	CC
cord-308835-999kewdw	17	7	have	have	VBP
cord-308835-999kewdw	17	8	been	be	VBN
cord-308835-999kewdw	17	9	associated	associate	VBN
cord-308835-999kewdw	17	10	with	with	IN
cord-308835-999kewdw	17	11	several	several	JJ
cord-308835-999kewdw	17	12	diseases	disease	NNS
cord-308835-999kewdw	17	13	in	in	IN
cord-308835-999kewdw	17	14	infected	infected	JJ
cord-308835-999kewdw	17	15	hosts	host	NNS
cord-308835-999kewdw	17	16	(	(	-LRB-
cord-308835-999kewdw	17	17	Robb	Robb	NNP
cord-308835-999kewdw	17	18	and	and	CC
cord-308835-999kewdw	17	19	Bond	Bond	NNP
cord-308835-999kewdw	17	20	,	,	,
cord-308835-999kewdw	17	21	1979a	1979a	CD
cord-308835-999kewdw	17	22	)	)	-RRB-
cord-308835-999kewdw	17	23	.	.	.
cord-308835-999kewdw	18	1	Coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	18	2	are	be	VBP
cord-308835-999kewdw	18	3	defined	define	VBN
cord-308835-999kewdw	18	4	as	as	IN
cord-308835-999kewdw	18	5	pleomorphic	pleomorphic	JJ
cord-308835-999kewdw	18	6	enveloped	envelop	VBN
cord-308835-999kewdw	18	7	particles	particle	NNS
cord-308835-999kewdw	18	8	about	about	RB
cord-308835-999kewdw	18	9	100	100	CD
cord-308835-999kewdw	18	10	nm	nm	NN
cord-308835-999kewdw	18	11	in	in	IN
cord-308835-999kewdw	18	12	diameter	diameter	NN
cord-308835-999kewdw	18	13	which	which	WDT
cord-308835-999kewdw	18	14	have	have	VBP
cord-308835-999kewdw	18	15	characteristic	characteristic	JJ
cord-308835-999kewdw	18	16	bul-	bul-	VBN
cord-308835-999kewdw	18	17	bous	bous	JJ
cord-308835-999kewdw	18	18	projections	projection	NNS
cord-308835-999kewdw	18	19	on	on	IN
cord-308835-999kewdw	18	20	their	-PRON-	PRP$
cord-308835-999kewdw	18	21	surface	surface	NN
cord-308835-999kewdw	18	22	,	,	,
cord-308835-999kewdw	18	23	bud	bud	NN
cord-308835-999kewdw	18	24	through	through	IN
cord-308835-999kewdw	18	25	the	the	DT
cord-308835-999kewdw	18	26	endoplasmic	endoplasmic	NN
cord-308835-999kewdw	18	27	reticulum	reticulum	NN
cord-308835-999kewdw	18	28	rather	rather	RB
cord-308835-999kewdw	18	29	than	than	IN
cord-308835-999kewdw	18	30	the	the	DT
cord-308835-999kewdw	18	31	plasma	plasma	NN
cord-308835-999kewdw	18	32	membrane	membrane	NN
cord-308835-999kewdw	18	33	,	,	,
cord-308835-999kewdw	18	34	and	and	CC
cord-308835-999kewdw	18	35	contain	contain	VB
cord-308835-999kewdw	18	36	RNA	RNA	NNP
cord-308835-999kewdw	18	37	(	(	-LRB-
cord-308835-999kewdw	18	38	Tyrell	Tyrell	NNP
cord-308835-999kewdw	18	39	et	et	NNP
cord-308835-999kewdw	18	40	al	al	NNP
cord-308835-999kewdw	18	41	.	.	NNP
cord-308835-999kewdw	18	42	,	,	,
cord-308835-999kewdw	18	43	1978	1978	CD
cord-308835-999kewdw	18	44	)	)	-RRB-
cord-308835-999kewdw	18	45	.	.	.
cord-308835-999kewdw	19	1	Several	several	JJ
cord-308835-999kewdw	19	2	studies	study	NNS
cord-308835-999kewdw	19	3	of	of	IN
cord-308835-999kewdw	19	4	coronavirus	coronavirus	NN
cord-308835-999kewdw	19	5	genomic	genomic	JJ
cord-308835-999kewdw	19	6	RNA	RNA	NNP
cord-308835-999kewdw	19	7	have	have	VBP
cord-308835-999kewdw	19	8	been	be	VBN
cord-308835-999kewdw	19	9	reported	report	VBN
cord-308835-999kewdw	19	10	(	(	-LRB-
cord-308835-999kewdw	19	11	Lomneczi	Lomneczi	NNP
cord-308835-999kewdw	19	12	,	,	,
cord-308835-999kewdw	19	13	197'7	197'7	CD
cord-308835-999kewdw	19	14	;	;	:
cord-308835-999kewdw	19	15	Lomneczi	Lomneczi	NNP
cord-308835-999kewdw	19	16	and	and	CC
cord-308835-999kewdw	19	17	Kennedy	Kennedy	NNP
cord-308835-999kewdw	19	18	,	,	,
cord-308835-999kewdw	19	19	1977	1977	CD
cord-308835-999kewdw	19	20	;	;	:
cord-308835-999kewdw	19	21	Yogo	Yogo	NNP
cord-308835-999kewdw	19	22	et	et	NNP
cord-308835-999kewdw	19	23	al	al	NNP
cord-308835-999kewdw	19	24	.	.	NNP
cord-308835-999kewdw	19	25	,	,	,
cord-308835-999kewdw	19	26	1977	1977	CD
cord-308835-999kewdw	19	27	;	;	:
cord-308835-999kewdw	19	28	Lai	Lai	NNP
cord-308835-999kewdw	19	29	and	and	CC
cord-308835-999kewdw	19	30	Stohlman	Stohlman	NNP
cord-308835-999kewdw	19	31	,	,	,
cord-308835-999kewdw	19	32	1978	1978	CD
cord-308835-999kewdw	19	33	;	;	:
cord-308835-999kewdw	19	34	Macnaughton	Macnaughton	NNP
cord-308835-999kewdw	19	35	and	and	CC
cord-308835-999kewdw	19	36	Madge	Madge	NNP
cord-308835-999kewdw	19	37	,	,	,
cord-308835-999kewdw	19	38	1978	1978	CD
cord-308835-999kewdw	19	39	;	;	:
cord-308835-999kewdw	19	40	Schochetman	Schochetman	NNP
cord-308835-999kewdw	19	41	et	et	NNP
cord-308835-999kewdw	19	42	al	al	NNP
cord-308835-999kewdw	19	43	.	.	NNP
cord-308835-999kewdw	19	44	,	,	,
cord-308835-999kewdw	19	45	1977	1977	CD
cord-308835-999kewdw	19	46	;	;	:
cord-308835-999kewdw	19	47	Tannock	Tannock	NNP
cord-308835-999kewdw	19	48	and	and	CC
cord-308835-999kewdw	19	49	Hierholzer	Hierholzer	NNP
cord-308835-999kewdw	19	50	,	,	,
cord-308835-999kewdw	19	51	1978	1978	CD
cord-308835-999kewdw	19	52	;	;	:
cord-308835-999kewdw	19	53	Guy	Guy	NNP
cord-308835-999kewdw	19	54	and	and	CC
cord-308835-999kewdw	19	55	Brian	Brian	NNP
cord-308835-999kewdw	19	56	,	,	,
cord-308835-999kewdw	19	57	1979	1979	CD
cord-308835-999kewdw	19	58	;	;	:
cord-308835-999kewdw	19	59	Wege	Wege	NNP
cord-308835-999kewdw	19	60	et	et	NNP
cord-308835-999kewdw	19	61	al	al	NNP
cord-308835-999kewdw	19	62	.	.	NNP
cord-308835-999kewdw	19	63	,	,	,
cord-308835-999kewdw	19	64	1978	1978	CD
cord-308835-999kewdw	19	65	)	)	-RRB-
cord-308835-999kewdw	19	66	.	.	.
cord-308835-999kewdw	20	1	These	these	DT
cord-308835-999kewdw	20	2	studies	study	NNS
cord-308835-999kewdw	20	3	have	have	VBP
cord-308835-999kewdw	20	4	indicated	indicate	VBN
cord-308835-999kewdw	20	5	that	that	IN
cord-308835-999kewdw	20	6	the	the	DT
cord-308835-999kewdw	20	7	coronavirus	coronavirus	NN
cord-308835-999kewdw	20	8	genome	genome	NN
cord-308835-999kewdw	20	9	is	be	VBZ
cord-308835-999kewdw	20	10	a	a	DT
cord-308835-999kewdw	20	11	large	large	JJ
cord-308835-999kewdw	20	12	(	(	-LRB-
cord-308835-999kewdw	20	13	5.4	5.4	CD
cord-308835-999kewdw	20	14	-	-	SYM
cord-308835-999kewdw	20	15	8.1	8.1	CD
cord-308835-999kewdw	20	16	X	x	NN
cord-308835-999kewdw	20	17	lo6	lo6	JJ
cord-308835-999kewdw	20	18	daltons	dalton	NNS
cord-308835-999kewdw	20	19	)	)	-RRB-
cord-308835-999kewdw	20	20	singlestranded	singlestrande	VBD
cord-308835-999kewdw	20	21	RNA	RNA	NNP
cord-308835-999kewdw	20	22	which	which	WDT
cord-308835-999kewdw	20	23	is	be	VBZ
cord-308835-999kewdw	20	24	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	20	25	and	and	CC
cord-308835-999kewdw	20	26	is	be	VBZ
cord-308835-999kewdw	20	27	infectious	infectious	JJ
cord-308835-999kewdw	20	28	.	.	.
cord-308835-999kewdw	21	1	These	these	DT
cord-308835-999kewdw	21	2	properties	property	NNS
cord-308835-999kewdw	21	3	identify	identify	VBP
cord-308835-999kewdw	21	4	the	the	DT
cord-308835-999kewdw	21	5	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	21	6	as	as	IN
cord-308835-999kewdw	21	7	positive	positive	JJ
cord-308835-999kewdw	21	8	-	-	HYPH
cord-308835-999kewdw	21	9	stranded	strand	VBN
cord-308835-999kewdw	21	10	RNA	RNA	NNP
cord-308835-999kewdw	21	11	viruses	virus	NNS
cord-308835-999kewdw	21	12	.	.	.
cord-308835-999kewdw	22	1	In	in	IN
cord-308835-999kewdw	22	2	contrast	contrast	NN
cord-308835-999kewdw	22	3	to	to	IN
cord-308835-999kewdw	22	4	the	the	DT
cord-308835-999kewdw	22	5	data	datum	NNS
cord-308835-999kewdw	22	6	obtained	obtain	VBN
cord-308835-999kewdw	22	7	on	on	IN
cord-308835-999kewdw	22	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	22	9	virion	virion	NN
cord-308835-999kewdw	22	10	RNA	RNA	NNP
cord-308835-999kewdw	22	11	,	,	,
cord-308835-999kewdw	22	12	few	few	JJ
cord-308835-999kewdw	22	13	data	datum	NNS
cord-308835-999kewdw	22	14	have	have	VBP
cord-308835-999kewdw	22	15	been	be	VBN
cord-308835-999kewdw	22	16	published	publish	VBN
cord-308835-999kewdw	22	17	on	on	IN
cord-308835-999kewdw	22	18	intracellular	intracellular	JJ
cord-308835-999kewdw	22	19	coronavirusspecific	coronavirusspecific	NNP
cord-308835-999kewdw	22	20	RNA	RNA	NNP
cord-308835-999kewdw	22	21	.	.	.
cord-308835-999kewdw	23	1	Mishra	Mishra	NNP
cord-308835-999kewdw	23	2	and	and	CC
cord-308835-999kewdw	23	3	Ryan	Ryan	NNP
cord-308835-999kewdw	23	4	(	(	-LRB-
cord-308835-999kewdw	23	5	1973	1973	CD
cord-308835-999kewdw	23	6	)	)	-RRB-
cord-308835-999kewdw	23	7	reported	report	VBD
cord-308835-999kewdw	23	8	that	that	IN
cord-308835-999kewdw	23	9	porcine	porcine	JJ
cord-308835-999kewdw	23	10	kidney	kidney	NN
cord-308835-999kewdw	23	11	cells	cell	NNS
cord-308835-999kewdw	23	12	infected	infect	VBN
cord-308835-999kewdw	23	13	with	with	IN
cord-308835-999kewdw	23	14	transmissible	transmissible	JJ
cord-308835-999kewdw	23	15	gastroenteritis	gastroenteritis	NN
cord-308835-999kewdw	23	16	virus	virus	NN
cord-308835-999kewdw	23	17	(	(	-LRB-
cord-308835-999kewdw	23	18	TGEV	TGEV	NNP
cord-308835-999kewdw	23	19	)	)	-RRB-
cord-308835-999kewdw	23	20	contained	contain	VBD
cord-308835-999kewdw	23	21	actinomycin	actinomycin	NNS
cord-308835-999kewdw	23	22	D	D	NNP
cord-308835-999kewdw	23	23	-	-	HYPH
cord-308835-999kewdw	23	24	resis	resis	NN
cord-308835-999kewdw	23	25	-	-	HYPH
cord-308835-999kewdw	23	26	tent	tent	NN
cord-308835-999kewdw	23	27	RNA	RNA	NNP
cord-308835-999kewdw	23	28	species	specie	NNS
cord-308835-999kewdw	23	29	which	which	WDT
cord-308835-999kewdw	23	30	sedimented	sediment	VBD
cord-308835-999kewdw	23	31	between	between	IN
cord-308835-999kewdw	23	32	18	18	CD
cord-308835-999kewdw	23	33	and	and	CC
cord-308835-999kewdw	23	34	28	28	CD
cord-308835-999kewdw	23	35	S.	S.	NNP
cord-308835-999kewdw	23	36	Robb	Robb	NNP
cord-308835-999kewdw	23	37	and	and	CC
cord-308835-999kewdw	23	38	Bond	Bond	NNP
cord-308835-999kewdw	23	39	(	(	-LRB-
cord-308835-999kewdw	23	40	1979b	1979b	CD
cord-308835-999kewdw	23	41	)	)	-RRB-
cord-308835-999kewdw	23	42	have	have	VBP
cord-308835-999kewdw	23	43	studied	study	VBN
cord-308835-999kewdw	23	44	murine	murine	JJ
cord-308835-999kewdw	23	45	hepatitis	hepatitis	NN
cord-308835-999kewdw	23	46	virus	virus	NN
cord-308835-999kewdw	23	47	(	(	-LRB-
cord-308835-999kewdw	23	48	MHV)-infected	mhv)-infected	JJ
cord-308835-999kewdw	23	49	cells	cell	NNS
cord-308835-999kewdw	23	50	and	and	CC
cord-308835-999kewdw	23	51	found	find	VBD
cord-308835-999kewdw	23	52	that	that	IN
cord-308835-999kewdw	23	53	deproteinized	deproteinize	VBN
cord-308835-999kewdw	23	54	RNA	RNA	NNP
cord-308835-999kewdw	23	55	from	from	IN
cord-308835-999kewdw	23	56	virus	virus	NN
cord-308835-999kewdw	23	57	-	-	HYPH
cord-308835-999kewdw	23	58	specific	specific	JJ
cord-308835-999kewdw	23	59	polysomes	polysome	NNS
cord-308835-999kewdw	23	60	sedimented	sediment	VBN
cord-308835-999kewdw	23	61	between	between	IN
cord-308835-999kewdw	23	62	10	10	CD
cord-308835-999kewdw	23	63	and	and	CC
cord-308835-999kewdw	23	64	28	28	CD
cord-308835-999kewdw	23	65	S.	S.	NNP
cord-308835-999kewdw	23	66	RNA	RNA	NNP
cord-308835-999kewdw	23	67	in	in	IN
cord-308835-999kewdw	23	68	this	this	DT
cord-308835-999kewdw	23	69	size	size	NN
cord-308835-999kewdw	23	70	range	range	NN
cord-308835-999kewdw	23	71	was	be	VBD
cord-308835-999kewdw	23	72	recently	recently	RB
cord-308835-999kewdw	23	73	demonstrated	demonstrate	VBN
cord-308835-999kewdw	23	74	to	to	TO
cord-308835-999kewdw	23	75	code	code	VB
cord-308835-999kewdw	23	76	for	for	IN
cord-308835-999kewdw	23	77	two	two	CD
cord-308835-999kewdw	23	78	MHV	MHV	NNP
cord-308835-999kewdw	23	79	structural	structural	JJ
cord-308835-999kewdw	23	80	proteins	protein	NNS
cord-308835-999kewdw	23	81	(	(	-LRB-
cord-308835-999kewdw	23	82	Siddell	Siddell	NNP
cord-308835-999kewdw	23	83	et	et	FW
cord-308835-999kewdw	23	84	al	al	NNP
cord-308835-999kewdw	23	85	.	.	NNP
cord-308835-999kewdw	23	86	,	,	,
cord-308835-999kewdw	23	87	1980	1980	CD
cord-308835-999kewdw	23	88	)	)	-RRB-
cord-308835-999kewdw	23	89	.	.	.
cord-308835-999kewdw	24	1	Stern	Stern	NNP
cord-308835-999kewdw	24	2	and	and	CC
cord-308835-999kewdw	24	3	Kennedy	Kennedy	NNP
cord-308835-999kewdw	24	4	(	(	-LRB-
cord-308835-999kewdw	24	5	1980a	1980a	CD
cord-308835-999kewdw	24	6	,	,	,
cord-308835-999kewdw	24	7	b	b	LS
cord-308835-999kewdw	24	8	)	)	-RRB-
cord-308835-999kewdw	24	9	have	have	VBP
cord-308835-999kewdw	24	10	identified	identify	VBN
cord-308835-999kewdw	24	11	six	six	CD
cord-308835-999kewdw	24	12	virus	virus	NN
cord-308835-999kewdw	24	13	-	-	HYPH
cord-308835-999kewdw	24	14	specific	specific	JJ
cord-308835-999kewdw	24	15	RNA	RNA	NNP
cord-308835-999kewdw	24	16	species	specie	NNS
cord-308835-999kewdw	24	17	in	in	IN
cord-308835-999kewdw	24	18	cells	cell	NNS
cord-308835-999kewdw	24	19	infected	infect	VBN
cord-308835-999kewdw	24	20	with	with	IN
cord-308835-999kewdw	24	21	avian	avian	JJ
cord-308835-999kewdw	24	22	infectious	infectious	JJ
cord-308835-999kewdw	24	23	bronchitis	bronchitis	NN
cord-308835-999kewdw	24	24	virus	virus	NN
cord-308835-999kewdw	24	25	(	(	-LRB-
cord-308835-999kewdw	24	26	IBV	IBV	NNP
cord-308835-999kewdw	24	27	)	)	-RRB-
cord-308835-999kewdw	24	28	.	.	.
cord-308835-999kewdw	25	1	We	-PRON-	PRP
cord-308835-999kewdw	25	2	have	have	VBP
cord-308835-999kewdw	25	3	been	be	VBN
cord-308835-999kewdw	25	4	studying	study	VBG
cord-308835-999kewdw	25	5	the	the	DT
cord-308835-999kewdw	25	6	virus	virus	NN
cord-308835-999kewdw	25	7	-	-	HYPH
cord-308835-999kewdw	25	8	specific	specific	JJ
cord-308835-999kewdw	25	9	RNA	RNA	NNP
cord-308835-999kewdw	25	10	species	specie	NNS
cord-308835-999kewdw	25	11	synthesized	synthesize	VBN
cord-308835-999kewdw	25	12	in	in	IN
cord-308835-999kewdw	25	13	cells	cell	NNS
cord-308835-999kewdw	25	14	infected	infect	VBN
cord-308835-999kewdw	25	15	with	with	IN
cord-308835-999kewdw	25	16	two	two	CD
cord-308835-999kewdw	25	17	strains	strain	NNS
cord-308835-999kewdw	25	18	of	of	IN
cord-308835-999kewdw	25	19	MHV	mhv	NN
cord-308835-999kewdw	25	20	:	:	:
cord-308835-999kewdw	25	21	MHV	MHV	NNP
cord-308835-999kewdw	25	22	-	-	HYPH
cord-308835-999kewdw	25	23	A59	A59	NNP
cord-308835-999kewdw	25	24	(	(	-LRB-
cord-308835-999kewdw	25	25	A59V	A59V	NNP
cord-308835-999kewdw	25	26	)	)	-RRB-
cord-308835-999kewdw	25	27	and	and	CC
cord-308835-999kewdw	25	28	MHV	MHV	NNP
cord-308835-999kewdw	25	29	-	-	HYPH
cord-308835-999kewdw	25	30	JHM	JHM	NNP
cord-308835-999kewdw	25	31	(	(	-LRB-
cord-308835-999kewdw	25	32	JHMV	JHMV	NNP
cord-308835-999kewdw	25	33	)	)	-RRB-
cord-308835-999kewdw	25	34	.	.	.
cord-308835-999kewdw	26	1	JHMV	JHMV	NNP
cord-308835-999kewdw	26	2	is	be	VBZ
cord-308835-999kewdw	26	3	highly	highly	RB
cord-308835-999kewdw	26	4	neurotropic	neurotropic	NNP
cord-308835-999kewdw	26	5	and	and	CC
cord-308835-999kewdw	26	6	produces	produce	VBZ
cord-308835-999kewdw	26	7	an	an	DT
cord-308835-999kewdw	26	8	encephalomyelitis	encephalomyelitis	NN
cord-308835-999kewdw	26	9	with	with	IN
cord-308835-999kewdw	26	10	demyelination	demyelination	NN
cord-308835-999kewdw	26	11	in	in	IN
cord-308835-999kewdw	26	12	its	-PRON-	PRP$
cord-308835-999kewdw	26	13	natural	natural	JJ
cord-308835-999kewdw	26	14	host	host	NN
cord-308835-999kewdw	26	15	,	,	,
cord-308835-999kewdw	26	16	the	the	DT
cord-308835-999kewdw	26	17	mouse	mouse	NN
cord-308835-999kewdw	26	18	(	(	-LRB-
cord-308835-999kewdw	26	19	Bailey	Bailey	NNP
cord-308835-999kewdw	26	20	et	et	NNP
cord-308835-999kewdw	26	21	al	al	NNP
cord-308835-999kewdw	26	22	.	.	NNP
cord-308835-999kewdw	26	23	,	,	,
cord-308835-999kewdw	26	24	1949	1949	CD
cord-308835-999kewdw	26	25	)	)	-RRB-
cord-308835-999kewdw	26	26	.	.	.
cord-308835-999kewdw	27	1	A59V	A59V	NNP
cord-308835-999kewdw	27	2	is	be	VBZ
cord-308835-999kewdw	27	3	weakly	weakly	RB
cord-308835-999kewdw	27	4	neurotropic	neurotropic	NNP
cord-308835-999kewdw	28	1	(	(	-LRB-
cord-308835-999kewdw	28	2	Robb	Robb	NNP
cord-308835-999kewdw	28	3	et	et	FW
cord-308835-999kewdw	28	4	al	al	NNP
cord-308835-999kewdw	28	5	.	.	NNP
cord-308835-999kewdw	28	6	,	,	,
cord-308835-999kewdw	28	7	1979	1979	CD
cord-308835-999kewdw	28	8	)	)	-RRB-
cord-308835-999kewdw	28	9	.	.	.
cord-308835-999kewdw	29	1	We	-PRON-	PRP
cord-308835-999kewdw	29	2	have	have	VBP
cord-308835-999kewdw	29	3	been	be	VBN
cord-308835-999kewdw	29	4	investigating	investigate	VBG
cord-308835-999kewdw	29	5	the	the	DT
cord-308835-999kewdw	29	6	replication	replication	NN
cord-308835-999kewdw	29	7	of	of	IN
cord-308835-999kewdw	29	8	these	these	DT
cord-308835-999kewdw	29	9	viruses	virus	NNS
cord-308835-999kewdw	29	10	in	in	IN
cord-308835-999kewdw	29	11	the	the	DT
cord-308835-999kewdw	29	12	hope	hope	NN
cord-308835-999kewdw	29	13	of	of	IN
cord-308835-999kewdw	29	14	eventually	eventually	RB
cord-308835-999kewdw	29	15	understanding	understand	VBG
cord-308835-999kewdw	29	16	the	the	DT
cord-308835-999kewdw	29	17	molecular	molecular	JJ
cord-308835-999kewdw	29	18	mechanisms	mechanism	NNS
cord-308835-999kewdw	29	19	by	by	IN
cord-308835-999kewdw	29	20	which	which	WDT
cord-308835-999kewdw	29	21	JHMV	JHMV	NNP
cord-308835-999kewdw	29	22	produces	produce	VBZ
cord-308835-999kewdw	29	23	demyelination	demyelination	NN
cord-308835-999kewdw	29	24	.	.	.
cord-308835-999kewdw	30	1	We	-PRON-	PRP
cord-308835-999kewdw	30	2	report	report	VBP
cord-308835-999kewdw	30	3	here	here	RB
cord-308835-999kewdw	30	4	the	the	DT
cord-308835-999kewdw	30	5	identification	identification	NN
cord-308835-999kewdw	30	6	of	of	IN
cord-308835-999kewdw	30	7	seven	seven	CD
cord-308835-999kewdw	30	8	major	major	JJ
cord-308835-999kewdw	30	9	MHV	mhv	NN
cord-308835-999kewdw	30	10	-	-	HYPH
cord-308835-999kewdw	30	11	specific	specific	JJ
cord-308835-999kewdw	30	12	intracellular	intracellular	JJ
cord-308835-999kewdw	30	13	RNA	RNA	NNP
cord-308835-999kewdw	30	14	species	specie	NNS
cord-308835-999kewdw	30	15	.	.	.
cord-308835-999kewdw	31	1	These	these	DT
cord-308835-999kewdw	31	2	RNAs	rna	NNS
cord-308835-999kewdw	31	3	have	have	VBP
cord-308835-999kewdw	31	4	been	be	VBN
cord-308835-999kewdw	31	5	characterized	characterize	VBN
cord-308835-999kewdw	31	6	by	by	IN
cord-308835-999kewdw	31	7	agarose	agarose	JJ
cord-308835-999kewdw	31	8	gel	gel	NN
cord-308835-999kewdw	31	9	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	31	10	,	,	,
cord-308835-999kewdw	31	11	poly(U	poly(u	ADD
cord-308835-999kewdw	31	12	)	)	-RRB-
cord-308835-999kewdw	32	1	Sepharose	sepharose	JJ
cord-308835-999kewdw	32	2	affinity	affinity	NN
cord-308835-999kewdw	32	3	chromatography	chromatography	NN
cord-308835-999kewdw	32	4	,	,	,
cord-308835-999kewdw	32	5	and	and	CC
cord-308835-999kewdw	32	6	oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	32	7	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	32	8	.	.	.
cord-308835-999kewdw	33	1	MATERIALS	materials	NN
cord-308835-999kewdw	33	2	AND	and	CC
cord-308835-999kewdw	33	3	METHODS	methods	NN
cord-308835-999kewdw	33	4	Materials	material	NNS
cord-308835-999kewdw	33	5	.	.	.
cord-308835-999kewdw	34	1	[	[	-LRB-
cord-308835-999kewdw	34	2	3H]Uridine	3h]uridine	NN
cord-308835-999kewdw	34	3	was	be	VBD
cord-308835-999kewdw	34	4	obtained	obtain	VBN
cord-308835-999kewdw	34	5	from	from	IN
cord-308835-999kewdw	34	6	New	New	NNP
cord-308835-999kewdw	34	7	England	England	NNP
cord-308835-999kewdw	34	8	Nuclear	Nuclear	NNP
cord-308835-999kewdw	34	9	and	and	CC
cord-308835-999kewdw	34	10	[	[	-LRB-
cord-308835-999kewdw	34	11	"	"	``
cord-308835-999kewdw	34	12	PIorthophosphate	piorthophosphate	JJ
cord-308835-999kewdw	34	13	(	(	-LRB-
cord-308835-999kewdw	34	14	carrier	carrier	NN
cord-308835-999kewdw	34	15	free	free	JJ
cord-308835-999kewdw	34	16	)	)	-RRB-
cord-308835-999kewdw	34	17	from	from	IN
cord-308835-999kewdw	34	18	New	New	NNP
cord-308835-999kewdw	34	19	England	England	NNP
cord-308835-999kewdw	34	20	Nuclear	Nuclear	NNP
cord-308835-999kewdw	34	21	,	,	,
cord-308835-999kewdw	34	22	Amersham	Amersham	NNP
cord-308835-999kewdw	34	23	,	,	,
cord-308835-999kewdw	34	24	or	or	CC
cord-308835-999kewdw	34	25	ICN	ICN	NNP
cord-308835-999kewdw	34	26	.	.	.
cord-308835-999kewdw	35	1	Phenol	phenol	NN
cord-308835-999kewdw	35	2	was	be	VBD
cord-308835-999kewdw	35	3	obtained	obtain	VBN
cord-308835-999kewdw	35	4	from	from	IN
cord-308835-999kewdw	35	5	Mallinckrodt	Mallinckrodt	NNP
cord-308835-999kewdw	35	6	and	and	CC
cord-308835-999kewdw	35	7	redistilled	redistille	VBD
cord-308835-999kewdw	35	8	prior	prior	RB
cord-308835-999kewdw	35	9	to	to	IN
cord-308835-999kewdw	35	10	use	use	NN
cord-308835-999kewdw	35	11	.	.	.
cord-308835-999kewdw	36	1	Actinomycin	Actinomycin	NNP
cord-308835-999kewdw	36	2	D	d	NN
cord-308835-999kewdw	36	3	and	and	CC
cord-308835-999kewdw	36	4	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	36	5	T1	T1	NNP
cord-308835-999kewdw	36	6	(	(	-LRB-
cord-308835-999kewdw	36	7	Sankyo	Sankyo	NNP
cord-308835-999kewdw	36	8	)	)	-RRB-
cord-308835-999kewdw	36	9	were	be	VBD
cord-308835-999kewdw	36	10	purchased	purchase	VBN
cord-308835-999kewdw	36	11	from	from	IN
cord-308835-999kewdw	36	12	Calbiochem	Calbiochem	NNP
cord-308835-999kewdw	36	13	and	and	CC
cord-308835-999kewdw	36	14	hexadecyltrimethylammonium	hexadecyltrimethylammonium	NNP
cord-308835-999kewdw	36	15	bromide	bromide	NN
cord-308835-999kewdw	36	16	(	(	-LRB-
cord-308835-999kewdw	36	17	CTAB	CTAB	NNP
cord-308835-999kewdw	36	18	)	)	-RRB-
cord-308835-999kewdw	36	19	and	and	CC
cord-308835-999kewdw	37	1	poly(U	poly(u	LS
cord-308835-999kewdw	37	2	)	)	-RRB-
cord-308835-999kewdw	38	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	38	2	from	from	IN
cord-308835-999kewdw	38	3	Sigma	Sigma	NNP
cord-308835-999kewdw	38	4	.	.	.
cord-308835-999kewdw	39	1	Glyoxal	Glyoxal	NNP
cord-308835-999kewdw	39	2	was	be	VBD
cord-308835-999kewdw	39	3	purchased	purchase	VBN
cord-308835-999kewdw	39	4	from	from	IN
cord-308835-999kewdw	39	5	Fisher	Fisher	NNP
cord-308835-999kewdw	39	6	Chemicals	Chemicals	NNPS
cord-308835-999kewdw	39	7	as	as	IN
cord-308835-999kewdw	39	8	a	a	DT
cord-308835-999kewdw	39	9	40	40	CD
cord-308835-999kewdw	39	10	%	%	NN
cord-308835-999kewdw	39	11	solution	solution	NN
cord-308835-999kewdw	39	12	and	and	CC
cord-308835-999kewdw	39	13	deionized	deionize	VBN
cord-308835-999kewdw	39	14	by	by	IN
cord-308835-999kewdw	39	15	the	the	DT
cord-308835-999kewdw	39	16	method	method	NN
cord-308835-999kewdw	39	17	of	of	IN
cord-308835-999kewdw	39	18	McMaster	McMaster	NNP
cord-308835-999kewdw	39	19	and	and	CC
cord-308835-999kewdw	39	20	Carmichael	Carmichael	NNP
cord-308835-999kewdw	39	21	(	(	-LRB-
cord-308835-999kewdw	39	22	1977	1977	CD
cord-308835-999kewdw	39	23	)	)	-RRB-
cord-308835-999kewdw	39	24	before	before	IN
cord-308835-999kewdw	39	25	use	use	NN
cord-308835-999kewdw	39	26	.	.	.
cord-308835-999kewdw	40	1	Methyl	methyl	NN
cord-308835-999kewdw	40	2	mercuric	mercuric	NN
cord-308835-999kewdw	40	3	hydroxide	hydroxide	NN
cord-308835-999kewdw	40	4	was	be	VBD
cord-308835-999kewdw	40	5	purchased	purchase	VBN
cord-308835-999kewdw	40	6	from	from	IN
cord-308835-999kewdw	40	7	Alpha	Alpha	NNP
cord-308835-999kewdw	40	8	Chemicals	Chemicals	NNPS
cord-308835-999kewdw	40	9	,	,	,
cord-308835-999kewdw	40	10	low	low	JJ
cord-308835-999kewdw	40	11	melting	melting	NN
cord-308835-999kewdw	40	12	point	point	NN
cord-308835-999kewdw	40	13	agarose	agarose	VBN
cord-308835-999kewdw	40	14	from	from	IN
cord-308835-999kewdw	40	15	Bethesda	Bethesda	NNP
cord-308835-999kewdw	40	16	Bioresearch	Bioresearch	NNP
cord-308835-999kewdw	40	17	Laboratories	Laboratories	NNPS
cord-308835-999kewdw	40	18	,	,	,
cord-308835-999kewdw	40	19	standard	standard	JJ
cord-308835-999kewdw	40	20	low	low	JJ
cord-308835-999kewdw	40	21	M	M	NNP
cord-308835-999kewdw	40	22	,	,	,
cord-308835-999kewdw	40	23	agarose	agarose	VBN
cord-308835-999kewdw	40	24	from	from	IN
cord-308835-999kewdw	40	25	Bio	Bio	NNP
cord-308835-999kewdw	40	26	-	-	HYPH
cord-308835-999kewdw	40	27	Rad	Rad	NNP
cord-308835-999kewdw	40	28	,	,	,
cord-308835-999kewdw	40	29	linear	linear	JJ
cord-308835-999kewdw	40	30	polyacrylamide	polyacrylamide	NN
cord-308835-999kewdw	40	31	from	from	IN
cord-308835-999kewdw	40	32	British	British	NNP
cord-308835-999kewdw	40	33	Drug	Drug	NNP
cord-308835-999kewdw	40	34	House	House	NNP
cord-308835-999kewdw	40	35	,	,	,
cord-308835-999kewdw	40	36	and	and	CC
cord-308835-999kewdw	40	37	proteinase	proteinase	NN
cord-308835-999kewdw	40	38	K	K	NNP
cord-308835-999kewdw	40	39	from	from	IN
cord-308835-999kewdw	40	40	Beckman	Beckman	NNP
cord-308835-999kewdw	40	41	Chemicals	Chemicals	NNP
cord-308835-999kewdw	40	42	.	.	.
cord-308835-999kewdw	41	1	Acrylamide	acrylamide	NN
cord-308835-999kewdw	41	2	,	,	,
cord-308835-999kewdw	41	3	xylene	xylene	NN
cord-308835-999kewdw	41	4	cyan01	cyan01	NNS
cord-308835-999kewdw	41	5	FF	ff	NN
cord-308835-999kewdw	41	6	,	,	,
cord-308835-999kewdw	41	7	and	and	CC
cord-308835-999kewdw	41	8	bromphenol	bromphenol	NNP
cord-308835-999kewdw	41	9	blue	blue	NNP
cord-308835-999kewdw	41	10	were	be	VBD
cord-308835-999kewdw	41	11	obtained	obtain	VBN
cord-308835-999kewdw	41	12	from	from	IN
cord-308835-999kewdw	41	13	Eastman	Eastman	NNP
cord-308835-999kewdw	41	14	Chemical	Chemical	NNP
cord-308835-999kewdw	41	15	.	.	.
cord-308835-999kewdw	42	1	Hydroxylapatite	Hydroxylapatite	NNP
cord-308835-999kewdw	42	2	(	(	-LRB-
cord-308835-999kewdw	42	3	HA	HA	NNP
cord-308835-999kewdw	42	4	;	;	:
cord-308835-999kewdw	42	5	DNA	dna	NN
cord-308835-999kewdw	42	6	grade	grade	NN
cord-308835-999kewdw	42	7	)	)	-RRB-
cord-308835-999kewdw	42	8	was	be	VBD
cord-308835-999kewdw	42	9	obtained	obtain	VBN
cord-308835-999kewdw	42	10	from	from	IN
cord-308835-999kewdw	42	11	Bio	Bio	NNP
cord-308835-999kewdw	42	12	-	-	HYPH
cord-308835-999kewdw	42	13	Rad	Rad	NNP
cord-308835-999kewdw	42	14	and	and	CC
cord-308835-999kewdw	42	15	prepared	prepare	VBD
cord-308835-999kewdw	42	16	as	as	IN
cord-308835-999kewdw	42	17	described	describe	VBN
cord-308835-999kewdw	42	18	by	by	IN
cord-308835-999kewdw	42	19	Stern	Stern	NNP
cord-308835-999kewdw	42	20	and	and	CC
cord-308835-999kewdw	42	21	Kennedy	Kennedy	NNP
cord-308835-999kewdw	42	22	(	(	-LRB-
cord-308835-999kewdw	42	23	1980a	1980a	CD
cord-308835-999kewdw	42	24	)	)	-RRB-
cord-308835-999kewdw	42	25	.	.	.
cord-308835-999kewdw	43	1	Cells	cell	NNS
cord-308835-999kewdw	43	2	.	.	.
cord-308835-999kewdw	44	1	The	the	DT
cord-308835-999kewdw	44	2	origin	origin	NN
cord-308835-999kewdw	44	3	and	and	CC
cord-308835-999kewdw	44	4	growth	growth	NN
cord-308835-999kewdw	44	5	of	of	IN
cord-308835-999kewdw	44	6	the	the	DT
cord-308835-999kewdw	44	7	murine	murine	JJ
cord-308835-999kewdw	44	8	cell	cell	NN
cord-308835-999kewdw	44	9	line	line	NN
cord-308835-999kewdw	44	10	17CL	17cl	CD
cord-308835-999kewdw	44	11	.	.	.
cord-308835-999kewdw	45	1	1	1	CD
cord-308835-999kewdw	45	2	has	have	VBZ
cord-308835-999kewdw	45	3	been	be	VBN
cord-308835-999kewdw	45	4	previously	previously	RB
cord-308835-999kewdw	45	5	described	describe	VBN
cord-308835-999kewdw	45	6	(	(	-LRB-
cord-308835-999kewdw	45	7	Sturman	Sturman	NNP
cord-308835-999kewdw	45	8	and	and	CC
cord-308835-999kewdw	45	9	Takemoto	Takemoto	NNP
cord-308835-999kewdw	45	10	,	,	,
cord-308835-999kewdw	45	11	1972	1972	CD
cord-308835-999kewdw	45	12	;	;	:
cord-308835-999kewdw	45	13	Bond	Bond	NNP
cord-308835-999kewdw	45	14	et	et	NNP
cord-308835-999kewdw	45	15	al	al	NNP
cord-308835-999kewdw	45	16	.	.	NNP
cord-308835-999kewdw	45	17	,	,	,
cord-308835-999kewdw	45	18	1979	1979	CD
cord-308835-999kewdw	45	19	)	)	-RRB-
cord-308835-999kewdw	45	20	.	.	.
cord-308835-999kewdw	46	1	HeLa	HeLa	NNP
cord-308835-999kewdw	46	2	cells	cell	NNS
cord-308835-999kewdw	46	3	were	be	VBD
cord-308835-999kewdw	46	4	obtained	obtain	VBN
cord-308835-999kewdw	46	5	from	from	IN
cord-308835-999kewdw	46	6	Dr.	Dr.	NNP
cord-308835-999kewdw	46	7	John	John	NNP
cord-308835-999kewdw	46	8	Holland	Holland	NNP
cord-308835-999kewdw	46	9	and	and	CC
cord-308835-999kewdw	46	10	grown	grow	VBN
cord-308835-999kewdw	46	11	in	in	IN
cord-308835-999kewdw	46	12	32	32	CD
cord-308835-999kewdw	46	13	-	-	HYPH
cord-308835-999kewdw	46	14	0~.	0~.	CD
cord-308835-999kewdw	46	15	prescription	prescription	NN
cord-308835-999kewdw	46	16	bottles	bottle	NNS
cord-308835-999kewdw	46	17	in	in	IN
cord-308835-999kewdw	46	18	Dulbecco	Dulbecco	NNP
cord-308835-999kewdw	46	19	's	's	POS
cord-308835-999kewdw	46	20	modified	modify	VBN
cord-308835-999kewdw	46	21	Eagle	Eagle	NNP
cord-308835-999kewdw	46	22	's	's	POS
cord-308835-999kewdw	46	23	medium	medium	NN
cord-308835-999kewdw	46	24	with	with	IN
cord-308835-999kewdw	46	25	10	10	CD
cord-308835-999kewdw	46	26	%	%	NN
cord-308835-999kewdw	46	27	calf	calf	NN
cord-308835-999kewdw	46	28	serum	serum	NN
cord-308835-999kewdw	46	29	.	.	.
cord-308835-999kewdw	47	1	Viirms	Viirms	NNP
cord-308835-999kewdw	47	2	.	.	.
cord-308835-999kewdw	48	1	The	the	DT
cord-308835-999kewdw	48	2	origin	origin	NN
cord-308835-999kewdw	48	3	and	and	CC
cord-308835-999kewdw	48	4	growth	growth	NN
cord-308835-999kewdw	48	5	of	of	IN
cord-308835-999kewdw	48	6	A59V	a59v	NN
cord-308835-999kewdw	48	7	and	and	CC
cord-308835-999kewdw	48	8	JHMV	JHMV	NNP
cord-308835-999kewdw	48	9	virus	virus	NN
cord-308835-999kewdw	48	10	stocks	stock	NNS
cord-308835-999kewdw	48	11	have	have	VBP
cord-308835-999kewdw	48	12	been	be	VBN
cord-308835-999kewdw	48	13	described	describe	VBN
cord-308835-999kewdw	48	14	(	(	-LRB-
cord-308835-999kewdw	48	15	Robb	Robb	NNP
cord-308835-999kewdw	48	16	and	and	CC
cord-308835-999kewdw	48	17	Bond	Bond	NNP
cord-308835-999kewdw	48	18	,	,	,
cord-308835-999kewdw	48	19	1979b	1979b	CD
cord-308835-999kewdw	48	20	)	)	-RRB-
cord-308835-999kewdw	48	21	.	.	.
cord-308835-999kewdw	49	1	For	for	IN
cord-308835-999kewdw	49	2	experiments	experiment	NNS
cord-308835-999kewdw	49	3	17CL	17cl	CD
cord-308835-999kewdw	49	4	.	.	.
cord-308835-999kewdw	50	1	1	1	CD
cord-308835-999kewdw	50	2	cells	cell	NNS
cord-308835-999kewdw	50	3	were	be	VBD
cord-308835-999kewdw	50	4	removed	remove	VBN
cord-308835-999kewdw	50	5	from	from	IN
cord-308835-999kewdw	50	6	the	the	DT
cord-308835-999kewdw	50	7	glass	glass	NN
cord-308835-999kewdw	50	8	substrate	substrate	NN
cord-308835-999kewdw	50	9	with	with	IN
cord-308835-999kewdw	50	10	0.1	0.1	CD
cord-308835-999kewdw	50	11	%	%	NN
cord-308835-999kewdw	50	12	twice	twice	RB
cord-308835-999kewdw	50	13	crystallized	crystallize	VBN
cord-308835-999kewdw	50	14	trypsin	trypsin	NN
cord-308835-999kewdw	50	15	in	in	IN
cord-308835-999kewdw	50	16	Puck	Puck	NNP
cord-308835-999kewdw	50	17	's	's	POS
cord-308835-999kewdw	50	18	saline	saline	NN
cord-308835-999kewdw	50	19	A	A	NNP
cord-308835-999kewdw	50	20	supplemented	supplement	VBN
cord-308835-999kewdw	50	21	with	with	IN
cord-308835-999kewdw	50	22	0.01	0.01	CD
cord-308835-999kewdw	50	23	M	M	NNP
cord-308835-999kewdw	50	24	Tris	Tris	NNP
cord-308835-999kewdw	50	25	(	(	-LRB-
cord-308835-999kewdw	50	26	pH	pH	NNP
cord-308835-999kewdw	50	27	8.1	8.1	CD
cord-308835-999kewdw	50	28	)	)	-RRB-
cord-308835-999kewdw	50	29	and	and	CC
cord-308835-999kewdw	50	30	0.1	0.1	CD
cord-308835-999kewdw	50	31	%	%	NN
cord-308835-999kewdw	50	32	EGTA	EGTA	NNP
cord-308835-999kewdw	50	33	[	[	-LRB-
cord-308835-999kewdw	50	34	ethyleneglycol	ethyleneglycol	NNP
cord-308835-999kewdw	50	35	-	-	HYPH
cord-308835-999kewdw	50	36	his@-amino	his@-amino	NNP
cord-308835-999kewdw	50	37	ethyl	ethyl	NN
cord-308835-999kewdw	50	38	ether)-N	ether)-N	VBN
cord-308835-999kewdw	50	39	,	,	,
cord-308835-999kewdw	50	40	llrtetraacetic	llrtetraacetic	JJ
cord-308835-999kewdw	50	41	acid	acid	NN
cord-308835-999kewdw	50	42	]	]	-RRB-
cord-308835-999kewdw	50	43	,	,	,
cord-308835-999kewdw	50	44	resuspended	resuspend	VBN
cord-308835-999kewdw	50	45	in	in	IN
cord-308835-999kewdw	50	46	Dulbecco	Dulbecco	NNP
cord-308835-999kewdw	50	47	's	's	POS
cord-308835-999kewdw	50	48	modified	modify	VBN
cord-308835-999kewdw	50	49	Eagle	Eagle	NNP
cord-308835-999kewdw	50	50	's	's	POS
cord-308835-999kewdw	50	51	medium	medium	NN
cord-308835-999kewdw	50	52	containing	contain	VBG
cord-308835-999kewdw	50	53	10	10	CD
cord-308835-999kewdw	50	54	%	%	NN
cord-308835-999kewdw	50	55	fetal	fetal	JJ
cord-308835-999kewdw	50	56	bovine	bovine	JJ
cord-308835-999kewdw	50	57	serum	serum	NN
cord-308835-999kewdw	50	58	(	(	-LRB-
cord-308835-999kewdw	50	59	DMElO	DMElO	NNP
cord-308835-999kewdw	50	60	)	)	-RRB-
cord-308835-999kewdw	50	61	,	,	,
cord-308835-999kewdw	50	62	centrifuged	centrifuge	VBN
cord-308835-999kewdw	50	63	at	at	IN
cord-308835-999kewdw	50	64	500	500	CD
cord-308835-999kewdw	50	65	g	g	NN
cord-308835-999kewdw	50	66	for	for	IN
cord-308835-999kewdw	50	67	2	2	CD
cord-308835-999kewdw	50	68	min	min	NN
cord-308835-999kewdw	50	69	and	and	CC
cord-308835-999kewdw	50	70	resuspended	resuspend	VBD
cord-308835-999kewdw	50	71	in	in	IN
cord-308835-999kewdw	50	72	medium	medium	NN
cord-308835-999kewdw	50	73	containing	contain	VBG
cord-308835-999kewdw	50	74	2	2	CD
cord-308835-999kewdw	50	75	%	%	NN
cord-308835-999kewdw	50	76	fetal	fetal	JJ
cord-308835-999kewdw	50	77	bovine	bovine	JJ
cord-308835-999kewdw	50	78	serum	serum	NN
cord-308835-999kewdw	50	79	(	(	-LRB-
cord-308835-999kewdw	50	80	DMEZ	DMEZ	NNP
cord-308835-999kewdw	50	81	)	)	-RRB-
cord-308835-999kewdw	50	82	.	.	.
cord-308835-999kewdw	51	1	Sufficient	sufficient	JJ
cord-308835-999kewdw	51	2	virus	virus	NN
cord-308835-999kewdw	51	3	was	be	VBD
cord-308835-999kewdw	51	4	added	add	VBN
cord-308835-999kewdw	51	5	to	to	TO
cord-308835-999kewdw	51	6	give	give	VB
cord-308835-999kewdw	51	7	a	a	DT
cord-308835-999kewdw	51	8	multiplicity	multiplicity	NN
cord-308835-999kewdw	51	9	of	of	IN
cord-308835-999kewdw	51	10	infection	infection	NN
cord-308835-999kewdw	51	11	(	(	-LRB-
cord-308835-999kewdw	51	12	m.o.i	m.o.i	NNP
cord-308835-999kewdw	51	13	.	.	NNP
cord-308835-999kewdw	51	14	)	)	-RRB-
cord-308835-999kewdw	51	15	between	between	IN
cord-308835-999kewdw	51	16	0.1	0.1	CD
cord-308835-999kewdw	51	17	and	and	CC
cord-308835-999kewdw	51	18	0.15	0.15	CD
cord-308835-999kewdw	51	19	plaque	plaque	NN
cord-308835-999kewdw	51	20	forming	form	VBG
cord-308835-999kewdw	51	21	units	unit	NNS
cord-308835-999kewdw	51	22	/	/	SYM
cord-308835-999kewdw	51	23	cell	cell	NN
cord-308835-999kewdw	51	24	.	.	.
cord-308835-999kewdw	52	1	Virus	virus	NN
cord-308835-999kewdw	52	2	adsorption	adsorption	NN
cord-308835-999kewdw	52	3	was	be	VBD
cord-308835-999kewdw	52	4	at	at	IN
cord-308835-999kewdw	52	5	37	37	CD
cord-308835-999kewdw	52	6	"	"	``
cord-308835-999kewdw	52	7	for	for	IN
cord-308835-999kewdw	52	8	30	30	CD
cord-308835-999kewdw	52	9	min	min	NN
cord-308835-999kewdw	52	10	.	.	.
cord-308835-999kewdw	53	1	Following	follow	VBG
cord-308835-999kewdw	53	2	adsorption	adsorption	NN
cord-308835-999kewdw	53	3	the	the	DT
cord-308835-999kewdw	53	4	cells	cell	NNS
cord-308835-999kewdw	53	5	were	be	VBD
cord-308835-999kewdw	53	6	centrifuged	centrifuge	VBN
cord-308835-999kewdw	53	7	at	at	IN
cord-308835-999kewdw	53	8	500	500	CD
cord-308835-999kewdw	53	9	g	g	NN
cord-308835-999kewdw	53	10	for	for	IN
cord-308835-999kewdw	53	11	2	2	CD
cord-308835-999kewdw	53	12	min	min	NN
cord-308835-999kewdw	53	13	,	,	,
cord-308835-999kewdw	53	14	resuspended	resuspend	VBN
cord-308835-999kewdw	53	15	in	in	IN
cord-308835-999kewdw	53	16	DME	DME	NNP
cord-308835-999kewdw	53	17	prewarmed	prewarme	VBN
cord-308835-999kewdw	53	18	to	to	IN
cord-308835-999kewdw	53	19	37	37	CD
cord-308835-999kewdw	53	20	"	"	''
cord-308835-999kewdw	53	21	,	,	,
cord-308835-999kewdw	53	22	and	and	CC
cord-308835-999kewdw	53	23	plated	plate	VBN
cord-308835-999kewdw	53	24	into	into	IN
cord-308835-999kewdw	53	25	35-mm	35-mm	CD
cord-308835-999kewdw	53	26	(	(	-LRB-
cord-308835-999kewdw	53	27	1.5	1.5	CD
cord-308835-999kewdw	53	28	X	x	CD
cord-308835-999kewdw	53	29	lo6	lo6	VBN
cord-308835-999kewdw	53	30	cells	cell	NNS
cord-308835-999kewdw	53	31	/	/	SYM
cord-308835-999kewdw	53	32	dish	dish	NN
cord-308835-999kewdw	53	33	)	)	-RRB-
cord-308835-999kewdw	53	34	,	,	,
cord-308835-999kewdw	53	35	60-mm	60-mm	NNP
cord-308835-999kewdw	53	36	(	(	-LRB-
cord-308835-999kewdw	53	37	5	5	CD
cord-308835-999kewdw	53	38	X	x	NN
cord-308835-999kewdw	53	39	lo6	lo6	NNS
cord-308835-999kewdw	53	40	cells/	cells/	CD
cord-308835-999kewdw	53	41	dish	dish	NN
cord-308835-999kewdw	53	42	)	)	-RRB-
cord-308835-999kewdw	53	43	,	,	,
cord-308835-999kewdw	53	44	lOO	lOO	NNP
cord-308835-999kewdw	53	45	-	-	HYPH
cord-308835-999kewdw	53	46	mm	mm	NNP
cord-308835-999kewdw	53	47	(	(	-LRB-
cord-308835-999kewdw	53	48	1.5	1.5	CD
cord-308835-999kewdw	53	49	x	x	SYM
cord-308835-999kewdw	53	50	lO'cells	lo'cell	NNS
cord-308835-999kewdw	53	51	/	/	SYM
cord-308835-999kewdw	53	52	dish	dish	NN
cord-308835-999kewdw	53	53	)	)	-RRB-
cord-308835-999kewdw	53	54	,	,	,
cord-308835-999kewdw	53	55	or	or	CC
cord-308835-999kewdw	53	56	150	150	CD
cord-308835-999kewdw	53	57	mm	mm	NN
cord-308835-999kewdw	53	58	(	(	-LRB-
cord-308835-999kewdw	53	59	4	4	CD
cord-308835-999kewdw	53	60	-	-	SYM
cord-308835-999kewdw	53	61	5	5	CD
cord-308835-999kewdw	53	62	X	x	SYM
cord-308835-999kewdw	53	63	10	10	CD
cord-308835-999kewdw	53	64	'	'	POS
cord-308835-999kewdw	53	65	cells	cell	NNS
cord-308835-999kewdw	53	66	/	/	SYM
cord-308835-999kewdw	53	67	dish	dish	NN
cord-308835-999kewdw	53	68	)	)	-RRB-
cord-308835-999kewdw	53	69	plastic	plastic	NN
cord-308835-999kewdw	53	70	culture	culture	NN
cord-308835-999kewdw	53	71	dishes	dish	NNS
cord-308835-999kewdw	53	72	and	and	CC
cord-308835-999kewdw	53	73	further	further	RB
cord-308835-999kewdw	53	74	incubated	incubate	VBN
cord-308835-999kewdw	53	75	at	at	IN
cord-308835-999kewdw	53	76	37	37	CD
cord-308835-999kewdw	53	77	"	"	''
cord-308835-999kewdw	53	78	.	.	.
cord-308835-999kewdw	54	1	For	for	IN
cord-308835-999kewdw	54	2	most	most	JJS
cord-308835-999kewdw	54	3	experiments	experiment	NNS
cord-308835-999kewdw	54	4	utilizing	utilize	VBG
cord-308835-999kewdw	54	5	[	[	-LRB-
cord-308835-999kewdw	54	6	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	54	7	as	as	IN
cord-308835-999kewdw	54	8	label	label	NN
cord-308835-999kewdw	54	9	the	the	DT
cord-308835-999kewdw	54	10	cells	cell	NNS
cord-308835-999kewdw	54	11	were	be	VBD
cord-308835-999kewdw	54	12	resuspended	resuspend	VBN
cord-308835-999kewdw	54	13	and	and	CC
cord-308835-999kewdw	54	14	plated	plate	VBN
cord-308835-999kewdw	54	15	in	in	IN
cord-308835-999kewdw	54	16	phosphate	phosphate	NN
cord-308835-999kewdw	54	17	-	-	HYPH
cord-308835-999kewdw	54	18	free	free	JJ
cord-308835-999kewdw	54	19	DME-2	DME-2	NNS
cord-308835-999kewdw	54	20	.	.	.
cord-308835-999kewdw	55	1	The	the	DT
cord-308835-999kewdw	55	2	strain	strain	NN
cord-308835-999kewdw	55	3	of	of	IN
cord-308835-999kewdw	55	4	mengovirus	mengovirus	NNP
cord-308835-999kewdw	55	5	used	use	VBN
cord-308835-999kewdw	55	6	was	be	VBD
cord-308835-999kewdw	55	7	that	that	IN
cord-308835-999kewdw	55	8	described	describe	VBN
cord-308835-999kewdw	55	9	by	by	IN
cord-308835-999kewdw	55	10	Plagemann	Plagemann	NNP
cord-308835-999kewdw	55	11	and	and	CC
cord-308835-999kewdw	55	12	Swim	Swim	NNP
cord-308835-999kewdw	55	13	(	(	-LRB-
cord-308835-999kewdw	55	14	1966	1966	CD
cord-308835-999kewdw	55	15	Extraction	extraction	NN
cord-308835-999kewdw	55	16	of	of	IN
cord-308835-999kewdw	55	17	intracellular	intracellular	JJ
cord-308835-999kewdw	55	18	RNA	RNA	NNP
cord-308835-999kewdw	55	19	.	.	.
cord-308835-999kewdw	56	1	Cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	56	2	extracts	extract	NNS
cord-308835-999kewdw	56	3	of	of	IN
cord-308835-999kewdw	56	4	infected	infected	JJ
cord-308835-999kewdw	56	5	or	or	CC
cord-308835-999kewdw	56	6	mock	mock	JJ
cord-308835-999kewdw	56	7	-	-	HYPH
cord-308835-999kewdw	56	8	infected	infected	JJ
cord-308835-999kewdw	56	9	17CL	17cl	CD
cord-308835-999kewdw	56	10	.	.	.
cord-308835-999kewdw	57	1	1	1	CD
cord-308835-999kewdw	57	2	cells	cell	NNS
cord-308835-999kewdw	57	3	were	be	VBD
cord-308835-999kewdw	57	4	prepared	prepared	JJ
cord-308835-999kewdw	57	5	using	use	VBG
cord-308835-999kewdw	57	6	NP-40	NP-40	NNP
cord-308835-999kewdw	57	7	(	(	-LRB-
cord-308835-999kewdw	57	8	Borun	Borun	NNP
cord-308835-999kewdw	57	9	et	et	NNP
cord-308835-999kewdw	57	10	al	al	NNP
cord-308835-999kewdw	57	11	.	.	NNP
cord-308835-999kewdw	57	12	,	,	,
cord-308835-999kewdw	57	13	1967	1967	CD
cord-308835-999kewdw	57	14	)	)	-RRB-
cord-308835-999kewdw	57	15	.	.	.
cord-308835-999kewdw	58	1	Monolayers	monolayer	NNS
cord-308835-999kewdw	58	2	were	be	VBD
cord-308835-999kewdw	58	3	rinsed	rinse	VBN
cord-308835-999kewdw	58	4	once	once	RB
cord-308835-999kewdw	58	5	with	with	IN
cord-308835-999kewdw	58	6	cold	cold	JJ
cord-308835-999kewdw	58	7	phosphatebuffered	phosphatebuffered	JJ
cord-308835-999kewdw	58	8	saline	saline	NN
cord-308835-999kewdw	58	9	(	(	-LRB-
cord-308835-999kewdw	58	10	PBS	PBS	NNP
cord-308835-999kewdw	58	11	)	)	-RRB-
cord-308835-999kewdw	58	12	,	,	,
cord-308835-999kewdw	58	13	scraped	scrape	VBN
cord-308835-999kewdw	58	14	into	into	IN
cord-308835-999kewdw	58	15	cold	cold	JJ
cord-308835-999kewdw	58	16	reticulocyte	reticulocyte	NN
cord-308835-999kewdw	58	17	standard	standard	NN
cord-308835-999kewdw	58	18	buffer	buffer	NN
cord-308835-999kewdw	58	19	(	(	-LRB-
cord-308835-999kewdw	58	20	RSB	RSB	NNP
cord-308835-999kewdw	58	21	,	,	,
cord-308835-999kewdw	58	22	0.01	0.01	CD
cord-308835-999kewdw	58	23	M	M	NNP
cord-308835-999kewdw	58	24	Tris	Tris	NNP
cord-308835-999kewdw	58	25	,	,	,
cord-308835-999kewdw	58	26	pH	pH	NNP
cord-308835-999kewdw	58	27	7.4,O.Ol	7.4,o.ol	NN
cord-308835-999kewdw	58	28	M	M	NNP
cord-308835-999kewdw	58	29	NaCl	NaCl	NNP
cord-308835-999kewdw	58	30	,	,	,
cord-308835-999kewdw	58	31	0.0015	0.0015	CD
cord-308835-999kewdw	58	32	M	M	NNP
cord-308835-999kewdw	58	33	MgCl	MgCl	NNP
cord-308835-999kewdw	58	34	.	.	.
cord-308835-999kewdw	58	35	&	&	CC
cord-308835-999kewdw	58	36	with	with	IN
cord-308835-999kewdw	58	37	a	a	DT
cord-308835-999kewdw	58	38	rubber	rubber	NN
cord-308835-999kewdw	58	39	policeman	policeman	NN
cord-308835-999kewdw	58	40	and	and	CC
cord-308835-999kewdw	58	41	transferred	transfer	VBD
cord-308835-999kewdw	58	42	to	to	IN
cord-308835-999kewdw	58	43	a	a	DT
cord-308835-999kewdw	58	44	tube	tube	NN
cord-308835-999kewdw	58	45	containing	contain	VBG
cord-308835-999kewdw	58	46	sufficient	sufficient	JJ
cord-308835-999kewdw	58	47	NP-40	NP-40	NNP
cord-308835-999kewdw	58	48	to	to	TO
cord-308835-999kewdw	58	49	give	give	VB
cord-308835-999kewdw	58	50	a	a	DT
cord-308835-999kewdw	58	51	final	final	JJ
cord-308835-999kewdw	58	52	concentration	concentration	NN
cord-308835-999kewdw	58	53	of	of	IN
cord-308835-999kewdw	58	54	1	1	CD
cord-308835-999kewdw	58	55	%	%	NN
cord-308835-999kewdw	58	56	.	.	.
cord-308835-999kewdw	59	1	The	the	DT
cord-308835-999kewdw	59	2	cell	cell	NN
cord-308835-999kewdw	59	3	suspension	suspension	NN
cord-308835-999kewdw	59	4	was	be	VBD
cord-308835-999kewdw	59	5	vortexed	vortexe	VBN
cord-308835-999kewdw	59	6	gently	gently	RB
cord-308835-999kewdw	59	7	,	,	,
cord-308835-999kewdw	59	8	incubated	incubate	VBN
cord-308835-999kewdw	59	9	on	on	IN
cord-308835-999kewdw	59	10	ice	ice	NN
cord-308835-999kewdw	59	11	for	for	IN
cord-308835-999kewdw	59	12	5	5	CD
cord-308835-999kewdw	59	13	min	min	NN
cord-308835-999kewdw	59	14	,	,	,
cord-308835-999kewdw	59	15	vortexed	vortexe	VBD
cord-308835-999kewdw	59	16	again	again	RB
cord-308835-999kewdw	59	17	,	,	,
cord-308835-999kewdw	59	18	and	and	CC
cord-308835-999kewdw	59	19	the	the	DT
cord-308835-999kewdw	59	20	nuclei	nucleus	NNS
cord-308835-999kewdw	59	21	removed	remove	VBN
cord-308835-999kewdw	59	22	by	by	IN
cord-308835-999kewdw	59	23	centrifugation	centrifugation	NN
cord-308835-999kewdw	59	24	at	at	IN
cord-308835-999kewdw	59	25	1500	1500	CD
cord-308835-999kewdw	59	26	g	g	NN
cord-308835-999kewdw	59	27	for	for	IN
cord-308835-999kewdw	59	28	2	2	CD
cord-308835-999kewdw	59	29	min	min	NN
cord-308835-999kewdw	59	30	.	.	.
cord-308835-999kewdw	60	1	The	the	DT
cord-308835-999kewdw	60	2	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	60	3	extract	extract	NN
cord-308835-999kewdw	60	4	was	be	VBD
cord-308835-999kewdw	60	5	adjusted	adjust	VBN
cord-308835-999kewdw	60	6	to	to	IN
cord-308835-999kewdw	60	7	1	1	CD
cord-308835-999kewdw	60	8	%	%	NN
cord-308835-999kewdw	60	9	SDS	SDS	NNP
cord-308835-999kewdw	60	10	,	,	,
cord-308835-999kewdw	60	11	0.4	0.4	CD
cord-308835-999kewdw	60	12	M	M	NNP
cord-308835-999kewdw	60	13	NaCl	NaCl	NNP
cord-308835-999kewdw	60	14	,	,	,
cord-308835-999kewdw	60	15	0.01	0.01	CD
cord-308835-999kewdw	60	16	M	M	NNP
cord-308835-999kewdw	60	17	EDTA	EDTA	NNP
cord-308835-999kewdw	60	18	,	,	,
cord-308835-999kewdw	60	19	and	and	CC
cord-308835-999kewdw	60	20	1.0	1.0	CD
cord-308835-999kewdw	60	21	mg	mg	NNP
cord-308835-999kewdw	60	22	/	/	SYM
cord-308835-999kewdw	60	23	ml	ml	NN
cord-308835-999kewdw	60	24	proteinase	proteinase	NN
cord-308835-999kewdw	60	25	K	K	NNP
cord-308835-999kewdw	60	26	and	and	CC
cord-308835-999kewdw	60	27	incubated	incubate	VBD
cord-308835-999kewdw	60	28	at	at	IN
cord-308835-999kewdw	60	29	50	50	CD
cord-308835-999kewdw	60	30	"	"	``
cord-308835-999kewdw	60	31	for	for	IN
cord-308835-999kewdw	60	32	5	5	CD
cord-308835-999kewdw	60	33	min	min	NN
cord-308835-999kewdw	60	34	and	and	CC
cord-308835-999kewdw	60	35	at	at	IN
cord-308835-999kewdw	60	36	room	room	NN
cord-308835-999kewdw	60	37	temperature	temperature	NN
cord-308835-999kewdw	60	38	for	for	IN
cord-308835-999kewdw	60	39	an	an	DT
cord-308835-999kewdw	60	40	additional	additional	JJ
cord-308835-999kewdw	60	41	25	25	CD
cord-308835-999kewdw	60	42	min	min	NN
cord-308835-999kewdw	60	43	.	.	.
cord-308835-999kewdw	61	1	The	the	DT
cord-308835-999kewdw	61	2	RNA	RNA	NNP
cord-308835-999kewdw	61	3	was	be	VBD
cord-308835-999kewdw	61	4	then	then	RB
cord-308835-999kewdw	61	5	extracted	extract	VBN
cord-308835-999kewdw	61	6	with	with	IN
cord-308835-999kewdw	61	7	phenol	phenol	NN
cord-308835-999kewdw	61	8	chloroform	chloroform	NN
cord-308835-999kewdw	61	9	(	(	-LRB-
cord-308835-999kewdw	61	10	Robb	Robb	NNP
cord-308835-999kewdw	61	11	and	and	CC
cord-308835-999kewdw	61	12	Bond	Bond	NNP
cord-308835-999kewdw	61	13	,	,	,
cord-308835-999kewdw	61	14	1979b	1979b	CD
cord-308835-999kewdw	61	15	)	)	-RRB-
cord-308835-999kewdw	61	16	and	and	CC
cord-308835-999kewdw	61	17	precipitated	precipitate	VBD
cord-308835-999kewdw	61	18	with	with	IN
cord-308835-999kewdw	61	19	3	3	CD
cord-308835-999kewdw	61	20	volumes	volume	NNS
cord-308835-999kewdw	61	21	of	of	IN
cord-308835-999kewdw	61	22	ethanol	ethanol	NN
cord-308835-999kewdw	61	23	at	at	IN
cord-308835-999kewdw	61	24	-20	-20	NNP
cord-308835-999kewdw	61	25	'	'	''
cord-308835-999kewdw	61	26	in	in	IN
cord-308835-999kewdw	61	27	the	the	DT
cord-308835-999kewdw	61	28	presence	presence	NN
cord-308835-999kewdw	61	29	of	of	IN
cord-308835-999kewdw	61	30	50	50	CD
cord-308835-999kewdw	61	31	pg	pg	NNS
cord-308835-999kewdw	61	32	of	of	IN
cord-308835-999kewdw	61	33	yeast	yeast	NN
cord-308835-999kewdw	61	34	carrier	carrier	NN
cord-308835-999kewdw	61	35	tRNA	tRNA	NNP
cord-308835-999kewdw	61	36	.	.	.
cord-308835-999kewdw	61	37	Preparation	preparation	NN
cord-308835-999kewdw	61	38	of	of	IN
cord-308835-999kewdw	61	39	MHVvirion	MHVvirion	NNP
cord-308835-999kewdw	61	40	RNA	RNA	NNP
cord-308835-999kewdw	61	41	.	.	.
cord-308835-999kewdw	62	1	17CL	17cl	CD
cord-308835-999kewdw	62	2	.	.	.
cord-308835-999kewdw	63	1	1	1	CD
cord-308835-999kewdw	63	2	cells	cell	NNS
cord-308835-999kewdw	63	3	were	be	VBD
cord-308835-999kewdw	63	4	infected	infect	VBN
cord-308835-999kewdw	63	5	with	with	IN
cord-308835-999kewdw	63	6	A59V	a59v	NN
cord-308835-999kewdw	63	7	or	or	CC
cord-308835-999kewdw	63	8	JHMV	JHMV	NNP
cord-308835-999kewdw	63	9	in	in	IN
cord-308835-999kewdw	63	10	phosphate	phosphate	NN
cord-308835-999kewdw	63	11	-	-	HYPH
cord-308835-999kewdw	63	12	free	free	JJ
cord-308835-999kewdw	63	13	DME2	DME2	NNP
cord-308835-999kewdw	63	14	as	as	IN
cord-308835-999kewdw	63	15	described	describe	VBN
cord-308835-999kewdw	63	16	above	above	RB
cord-308835-999kewdw	63	17	.	.	.
cord-308835-999kewdw	64	1	Following	follow	VBG
cord-308835-999kewdw	64	2	virus	virus	NN
cord-308835-999kewdw	64	3	adsorption	adsorption	NN
cord-308835-999kewdw	64	4	the	the	DT
cord-308835-999kewdw	64	5	cells	cell	NNS
cord-308835-999kewdw	64	6	were	be	VBD
cord-308835-999kewdw	64	7	plated	plate	VBN
cord-308835-999kewdw	64	8	in	in	IN
cord-308835-999kewdw	64	9	lOO	lOO	NNP
cord-308835-999kewdw	64	10	-	-	:
cord-308835-999kewdw	64	11	or	or	CC
cord-308835-999kewdw	64	12	150-mm	150-mm	CD
cord-308835-999kewdw	64	13	culture	culture	NN
cord-308835-999kewdw	64	14	dishes	dish	NNS
cord-308835-999kewdw	64	15	and	and	CC
cord-308835-999kewdw	64	16	incubated	incubate	VBN
cord-308835-999kewdw	64	17	at	at	IN
cord-308835-999kewdw	64	18	33	33	CD
cord-308835-999kewdw	64	19	"	"	''
cord-308835-999kewdw	64	20	.	.	.
cord-308835-999kewdw	65	1	At	at	IN
cord-308835-999kewdw	65	2	4	4	CD
cord-308835-999kewdw	65	3	hr	hr	NN
cord-308835-999kewdw	65	4	postinfection	postinfection	NN
cord-308835-999kewdw	65	5	(	(	-LRB-
cord-308835-999kewdw	65	6	hpi	hpi	NNP
cord-308835-999kewdw	65	7	)	)	-RRB-
cord-308835-999kewdw	65	8	[	[	-LRB-
cord-308835-999kewdw	65	9	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	65	10	was	be	VBD
cord-308835-999kewdw	65	11	added	add	VBN
cord-308835-999kewdw	65	12	to	to	IN
cord-308835-999kewdw	65	13	a	a	DT
cord-308835-999kewdw	65	14	concentration	concentration	NN
cord-308835-999kewdw	65	15	of	of	IN
cord-308835-999kewdw	65	16	100	100	CD
cord-308835-999kewdw	65	17	-	-	SYM
cord-308835-999kewdw	65	18	1000	1000	CD
cord-308835-999kewdw	65	19	&	&	CC
cord-308835-999kewdw	65	20	i/	i/	NNP
cord-308835-999kewdw	65	21	ml	ml	NNP
cord-308835-999kewdw	65	22	.	.	.
cord-308835-999kewdw	66	1	At	at	IN
cord-308835-999kewdw	66	2	16	16	CD
cord-308835-999kewdw	66	3	-	-	SYM
cord-308835-999kewdw	66	4	18	18	CD
cord-308835-999kewdw	66	5	hpi	hpi	NNP
cord-308835-999kewdw	66	6	the	the	DT
cord-308835-999kewdw	66	7	cell	cell	NN
cord-308835-999kewdw	66	8	-	-	HYPH
cord-308835-999kewdw	66	9	associated	associate	VBN
cord-308835-999kewdw	66	10	virus	virus	NN
cord-308835-999kewdw	66	11	was	be	VBD
cord-308835-999kewdw	66	12	released	release	VBN
cord-308835-999kewdw	66	13	by	by	IN
cord-308835-999kewdw	66	14	two	two	CD
cord-308835-999kewdw	66	15	cycles	cycle	NNS
cord-308835-999kewdw	66	16	of	of	IN
cord-308835-999kewdw	66	17	freeze	freeze	NN
cord-308835-999kewdw	66	18	thawing	thawing	NN
cord-308835-999kewdw	66	19	and	and	CC
cord-308835-999kewdw	66	20	the	the	DT
cord-308835-999kewdw	66	21	resulting	result	VBG
cord-308835-999kewdw	66	22	lysate	lysate	NN
cord-308835-999kewdw	66	23	clarified	clarify	VBN
cord-308835-999kewdw	66	24	by	by	IN
cord-308835-999kewdw	66	25	centrifugation	centrifugation	NN
cord-308835-999kewdw	66	26	at	at	IN
cord-308835-999kewdw	66	27	10,000	10,000	CD
cord-308835-999kewdw	66	28	g	g	NN
cord-308835-999kewdw	66	29	for	for	IN
cord-308835-999kewdw	66	30	30	30	CD
cord-308835-999kewdw	66	31	min	min	NN
cord-308835-999kewdw	66	32	at	at	IN
cord-308835-999kewdw	66	33	4	4	CD
cord-308835-999kewdw	66	34	'	'	''
cord-308835-999kewdw	66	35	.	.	.
cord-308835-999kewdw	67	1	Virus	virus	NN
cord-308835-999kewdw	67	2	was	be	VBD
cord-308835-999kewdw	67	3	concentrated	concentrate	VBN
cord-308835-999kewdw	67	4	by	by	IN
cord-308835-999kewdw	67	5	centrifugation	centrifugation	NN
cord-308835-999kewdw	67	6	for	for	IN
cord-308835-999kewdw	67	7	60	60	CD
cord-308835-999kewdw	67	8	min	min	NN
cord-308835-999kewdw	67	9	at	at	IN
cord-308835-999kewdw	67	10	35,000	35,000	CD
cord-308835-999kewdw	67	11	rpm	rpm	NN
cord-308835-999kewdw	67	12	in	in	IN
cord-308835-999kewdw	67	13	the	the	DT
cord-308835-999kewdw	67	14	SW40	SW40	NNP
cord-308835-999kewdw	67	15	rotor	rotor	NN
cord-308835-999kewdw	67	16	through	through	IN
cord-308835-999kewdw	67	17	a	a	DT
cord-308835-999kewdw	67	18	0.5-ml	0.5-ml	CD
cord-308835-999kewdw	67	19	pad	pad	NN
cord-308835-999kewdw	67	20	of	of	IN
cord-308835-999kewdw	67	21	15	15	CD
cord-308835-999kewdw	67	22	%	%	NN
cord-308835-999kewdw	67	23	(	(	-LRB-
cord-308835-999kewdw	67	24	w	w	NNP
cord-308835-999kewdw	67	25	/	/	SYM
cord-308835-999kewdw	67	26	w	w	NN
cord-308835-999kewdw	67	27	)	)	-RRB-
cord-308835-999kewdw	67	28	potassium	potassium	NN
cord-308835-999kewdw	67	29	tartrate	tartrate	NN
cord-308835-999kewdw	67	30	in	in	IN
cord-308835-999kewdw	67	31	MSE	MSE	NNP
cord-308835-999kewdw	67	32	buffer	buffer	NN
cord-308835-999kewdw	67	33	(	(	-LRB-
cord-308835-999kewdw	67	34	0.01	0.01	CD
cord-308835-999kewdw	67	35	M	M	NNP
cord-308835-999kewdw	67	36	morpholinopropane	morpholinopropane	NN
cord-308835-999kewdw	67	37	sulfonic	sulfonic	JJ
cord-308835-999kewdw	67	38	acid	acid	NN
cord-308835-999kewdw	67	39	,	,	,
cord-308835-999kewdw	67	40	0.15	0.15	CD
cord-308835-999kewdw	67	41	MNaCl	MNaCl	NNS
cord-308835-999kewdw	67	42	,	,	,
cord-308835-999kewdw	68	1	0.001	0.001	CD
cord-308835-999kewdw	68	2	M	M	NNP
cord-308835-999kewdw	68	3	EDTA	EDTA	NNP
cord-308835-999kewdw	68	4	,	,	,
cord-308835-999kewdw	68	5	pH	pH	NNP
cord-308835-999kewdw	68	6	6.8	6.8	CD
cord-308835-999kewdw	68	7	)	)	-RRB-
cord-308835-999kewdw	68	8	.	.	.
cord-308835-999kewdw	69	1	The	the	DT
cord-308835-999kewdw	69	2	virus	virus	NN
cord-308835-999kewdw	69	3	pellets	pellet	NNS
cord-308835-999kewdw	69	4	were	be	VBD
cord-308835-999kewdw	69	5	resuspended	resuspend	VBN
cord-308835-999kewdw	69	6	by	by	IN
cord-308835-999kewdw	69	7	sonication	sonication	NN
cord-308835-999kewdw	69	8	in	in	IN
cord-308835-999kewdw	69	9	0.5	0.5	CD
cord-308835-999kewdw	69	10	ml	ml	NNS
cord-308835-999kewdw	69	11	of	of	IN
cord-308835-999kewdw	69	12	MSE	MSE	NNP
cord-308835-999kewdw	69	13	buffer	buffer	NN
cord-308835-999kewdw	69	14	,	,	,
cord-308835-999kewdw	69	15	layered	layer	VBN
cord-308835-999kewdw	69	16	onto	onto	IN
cord-308835-999kewdw	69	17	a	a	DT
cord-308835-999kewdw	69	18	12	12	CD
cord-308835-999kewdw	69	19	ml	ml	NN
cord-308835-999kewdw	69	20	gradient	gradient	NN
cord-308835-999kewdw	69	21	of	of	IN
cord-308835-999kewdw	69	22	5	5	CD
cord-308835-999kewdw	69	23	-	-	SYM
cord-308835-999kewdw	69	24	25	25	CD
cord-308835-999kewdw	69	25	%	%	NN
cord-308835-999kewdw	69	26	(	(	-LRB-
cord-308835-999kewdw	69	27	w	w	NNP
cord-308835-999kewdw	69	28	/	/	SYM
cord-308835-999kewdw	69	29	w	w	NN
cord-308835-999kewdw	69	30	)	)	-RRB-
cord-308835-999kewdw	69	31	potassium	potassium	NN
cord-308835-999kewdw	69	32	tartrate	tartrate	NN
cord-308835-999kewdw	69	33	in	in	IN
cord-308835-999kewdw	69	34	MSE	MSE	NNP
cord-308835-999kewdw	69	35	buffer	buffer	NN
cord-308835-999kewdw	69	36	,	,	,
cord-308835-999kewdw	69	37	and	and	CC
cord-308835-999kewdw	69	38	centrifuged	centrifuge	VBD
cord-308835-999kewdw	69	39	at	at	IN
cord-308835-999kewdw	69	40	35,000	35,000	CD
cord-308835-999kewdw	69	41	rpm	rpm	NN
cord-308835-999kewdw	69	42	for	for	IN
cord-308835-999kewdw	69	43	45	45	CD
cord-308835-999kewdw	69	44	min	min	NNS
cord-308835-999kewdw	69	45	in	in	IN
cord-308835-999kewdw	69	46	the	the	DT
cord-308835-999kewdw	69	47	SW40	SW40	NNP
cord-308835-999kewdw	69	48	rotor	rotor	NN
cord-308835-999kewdw	69	49	.	.	.
cord-308835-999kewdw	70	1	The	the	DT
cord-308835-999kewdw	70	2	gradient	gradient	NN
cord-308835-999kewdw	70	3	was	be	VBD
cord-308835-999kewdw	70	4	fractionated	fractionate	VBN
cord-308835-999kewdw	70	5	and	and	CC
cord-308835-999kewdw	70	6	the	the	DT
cord-308835-999kewdw	70	7	virion	virion	NN
cord-308835-999kewdw	70	8	peak	peak	NN
cord-308835-999kewdw	70	9	located	locate	VBN
cord-308835-999kewdw	70	10	by	by	IN
cord-308835-999kewdw	70	11	counting	count	VBG
cord-308835-999kewdw	70	12	aliquots	aliquot	NNS
cord-308835-999kewdw	70	13	of	of	IN
cord-308835-999kewdw	70	14	each	each	DT
cord-308835-999kewdw	70	15	fraction	fraction	NN
cord-308835-999kewdw	70	16	.	.	.
cord-308835-999kewdw	71	1	For	for	IN
cord-308835-999kewdw	71	2	the	the	DT
cord-308835-999kewdw	71	3	preparation	preparation	NN
cord-308835-999kewdw	71	4	of	of	IN
cord-308835-999kewdw	71	5	highly	highly	RB
cord-308835-999kewdw	71	6	purified	purify	VBN
cord-308835-999kewdw	71	7	virus	virus	NN
cord-308835-999kewdw	71	8	this	this	DT
cord-308835-999kewdw	71	9	material	material	NN
cord-308835-999kewdw	71	10	was	be	VBD
cord-308835-999kewdw	71	11	diluted	dilute	VBN
cord-308835-999kewdw	71	12	with	with	IN
cord-308835-999kewdw	71	13	MSE	MSE	NNP
cord-308835-999kewdw	71	14	,	,	,
cord-308835-999kewdw	71	15	layered	layer	VBN
cord-308835-999kewdw	71	16	on	on	IN
cord-308835-999kewdw	71	17	a	a	DT
cord-308835-999kewdw	71	18	9	9	CD
cord-308835-999kewdw	71	19	ml	ml	NN
cord-308835-999kewdw	71	20	lo-40	lo-40	NNP
cord-308835-999kewdw	71	21	%	%	NN
cord-308835-999kewdw	71	22	(	(	-LRB-
cord-308835-999kewdw	71	23	w/	w/	NNP
cord-308835-999kewdw	71	24	w	w	NNP
cord-308835-999kewdw	71	25	)	)	-RRB-
cord-308835-999kewdw	71	26	potassium	potassium	NN
cord-308835-999kewdw	71	27	tartrate	tartrate	NN
cord-308835-999kewdw	71	28	gradient	gradient	NN
cord-308835-999kewdw	71	29	,	,	,
cord-308835-999kewdw	71	30	and	and	CC
cord-308835-999kewdw	71	31	centrifuged	centrifuge	VBN
cord-308835-999kewdw	71	32	in	in	IN
cord-308835-999kewdw	71	33	the	the	DT
cord-308835-999kewdw	71	34	SW40	SW40	NNP
cord-308835-999kewdw	71	35	rotor	rotor	NN
cord-308835-999kewdw	71	36	at	at	IN
cord-308835-999kewdw	71	37	37,090	37,090	CD
cord-308835-999kewdw	71	38	rpm	rpm	NN
cord-308835-999kewdw	71	39	for	for	IN
cord-308835-999kewdw	71	40	4	4	CD
cord-308835-999kewdw	71	41	hr	hr	NN
cord-308835-999kewdw	71	42	.	.	.
cord-308835-999kewdw	72	1	The	the	DT
cord-308835-999kewdw	72	2	gradient	gradient	NN
cord-308835-999kewdw	72	3	was	be	VBD
cord-308835-999kewdw	72	4	fractionated	fractionate	VBN
cord-308835-999kewdw	72	5	,	,	,
cord-308835-999kewdw	72	6	aliquots	aliquot	NNS
cord-308835-999kewdw	72	7	were	be	VBD
cord-308835-999kewdw	72	8	counted	count	VBN
cord-308835-999kewdw	72	9	,	,	,
cord-308835-999kewdw	72	10	and	and	CC
cord-308835-999kewdw	72	11	the	the	DT
cord-308835-999kewdw	72	12	peak	peak	NN
cord-308835-999kewdw	72	13	of	of	IN
cord-308835-999kewdw	72	14	radiolabeled	radiolabele	VBN
cord-308835-999kewdw	72	15	virus	virus	NN
cord-308835-999kewdw	72	16	collected	collect	VBN
cord-308835-999kewdw	72	17	.	.	.
cord-308835-999kewdw	73	1	This	this	DT
cord-308835-999kewdw	73	2	material	material	NN
cord-308835-999kewdw	73	3	had	have	VBD
cord-308835-999kewdw	73	4	a	a	DT
cord-308835-999kewdw	73	5	buoyant	buoyant	JJ
cord-308835-999kewdw	73	6	density	density	NN
cord-308835-999kewdw	73	7	between	between	IN
cord-308835-999kewdw	73	8	1.19	1.19	CD
cord-308835-999kewdw	73	9	and	and	CC
cord-308835-999kewdw	73	10	1.17	1.17	CD
cord-308835-999kewdw	73	11	g	g	NN
cord-308835-999kewdw	73	12	/	/	SYM
cord-308835-999kewdw	73	13	cm3	cm3	NNP
cord-308835-999kewdw	73	14	.	.	.
cord-308835-999kewdw	74	1	The	the	DT
cord-308835-999kewdw	74	2	virus	virus	NN
cord-308835-999kewdw	74	3	was	be	VBD
cord-308835-999kewdw	74	4	diluted	dilute	VBN
cord-308835-999kewdw	74	5	with	with	IN
cord-308835-999kewdw	74	6	MSE	MSE	NNP
cord-308835-999kewdw	74	7	buffer	buffer	NN
cord-308835-999kewdw	74	8	,	,	,
cord-308835-999kewdw	74	9	pelleted	pellete	VBN
cord-308835-999kewdw	74	10	at	at	IN
cord-308835-999kewdw	74	11	45,000	45,000	CD
cord-308835-999kewdw	74	12	rpm	rpm	NN
cord-308835-999kewdw	74	13	for	for	IN
cord-308835-999kewdw	74	14	30	30	CD
cord-308835-999kewdw	74	15	min	min	NN
cord-308835-999kewdw	74	16	in	in	IN
cord-308835-999kewdw	74	17	the	the	DT
cord-308835-999kewdw	74	18	SW50.1	sw50.1	JJ
cord-308835-999kewdw	74	19	rotor	rotor	NN
cord-308835-999kewdw	74	20	,	,	,
cord-308835-999kewdw	74	21	and	and	CC
cord-308835-999kewdw	74	22	resuspended	resuspend	VBN
cord-308835-999kewdw	74	23	by	by	IN
cord-308835-999kewdw	74	24	sonication	sonication	NN
cord-308835-999kewdw	74	25	in	in	IN
cord-308835-999kewdw	74	26	1	1	CD
cord-308835-999kewdw	74	27	ml	ml	NNS
cord-308835-999kewdw	74	28	of	of	IN
cord-308835-999kewdw	74	29	MSE	MSE	NNP
cord-308835-999kewdw	74	30	buffer	buffer	NN
cord-308835-999kewdw	74	31	.	.	.
cord-308835-999kewdw	75	1	Virion	Virion	NNP
cord-308835-999kewdw	75	2	RNA	RNA	NNP
cord-308835-999kewdw	75	3	was	be	VBD
cord-308835-999kewdw	75	4	extracted	extract	VBN
cord-308835-999kewdw	75	5	in	in	IN
cord-308835-999kewdw	75	6	a	a	DT
cord-308835-999kewdw	75	7	similar	similar	JJ
cord-308835-999kewdw	75	8	manner	manner	NN
cord-308835-999kewdw	75	9	to	to	IN
cord-308835-999kewdw	75	10	intracellular	intracellular	JJ
cord-308835-999kewdw	75	11	RNA	RNA	NNP
cord-308835-999kewdw	75	12	and	and	CC
cord-308835-999kewdw	75	13	precipitated	precipitate	VBN
cord-308835-999kewdw	75	14	with	with	IN
cord-308835-999kewdw	75	15	ethanol	ethanol	NN
cord-308835-999kewdw	75	16	after	after	IN
cord-308835-999kewdw	75	17	the	the	DT
cord-308835-999kewdw	75	18	addition	addition	NN
cord-308835-999kewdw	75	19	of	of	IN
cord-308835-999kewdw	75	20	50	50	CD
cord-308835-999kewdw	75	21	rg	rg	NNS
cord-308835-999kewdw	75	22	of	of	IN
cord-308835-999kewdw	75	23	carrier	carrier	NN
cord-308835-999kewdw	75	24	tRNA	tRNA	NNP
cord-308835-999kewdw	75	25	.	.	.
cord-308835-999kewdw	75	26	Virion	Virion	NNP
cord-308835-999kewdw	75	27	RNA	RNA	NNP
cord-308835-999kewdw	75	28	for	for	IN
cord-308835-999kewdw	75	29	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	75	30	was	be	VBD
cord-308835-999kewdw	75	31	prepared	prepare	VBN
cord-308835-999kewdw	75	32	by	by	IN
cord-308835-999kewdw	75	33	a	a	DT
cord-308835-999kewdw	75	34	slightly	slightly	RB
cord-308835-999kewdw	75	35	different	different	JJ
cord-308835-999kewdw	75	36	procedure	procedure	NN
cord-308835-999kewdw	75	37	.	.	.
cord-308835-999kewdw	76	1	Virus	virus	NN
cord-308835-999kewdw	76	2	was	be	VBD
cord-308835-999kewdw	76	3	concentrated	concentrate	VBN
cord-308835-999kewdw	76	4	and	and	CC
cord-308835-999kewdw	76	5	banded	band	VBN
cord-308835-999kewdw	76	6	on	on	IN
cord-308835-999kewdw	76	7	a	a	DT
cord-308835-999kewdw	76	8	5	5	CD
cord-308835-999kewdw	76	9	-	-	SYM
cord-308835-999kewdw	76	10	25	25	CD
cord-308835-999kewdw	76	11	%	%	NN
cord-308835-999kewdw	76	12	potassium	potassium	NN
cord-308835-999kewdw	76	13	tartrate	tartrate	NN
cord-308835-999kewdw	76	14	gradient	gradient	NN
cord-308835-999kewdw	76	15	and	and	CC
cord-308835-999kewdw	76	16	this	this	DT
cord-308835-999kewdw	76	17	partially	partially	RB
cord-308835-999kewdw	76	18	purified	purify	VBN
cord-308835-999kewdw	76	19	virus	virus	NN
cord-308835-999kewdw	76	20	was	be	VBD
cord-308835-999kewdw	76	21	diluted	dilute	VBN
cord-308835-999kewdw	76	22	with	with	IN
cord-308835-999kewdw	76	23	MSE	MSE	NNP
cord-308835-999kewdw	76	24	buffer	buffer	NN
cord-308835-999kewdw	76	25	,	,	,
cord-308835-999kewdw	76	26	pelleted	pellete	VBN
cord-308835-999kewdw	76	27	in	in	IN
cord-308835-999kewdw	76	28	the	the	DT
cord-308835-999kewdw	76	29	SW50.1	sw50.1	JJ
cord-308835-999kewdw	76	30	rotor	rotor	NN
cord-308835-999kewdw	76	31	,	,	,
cord-308835-999kewdw	76	32	and	and	CC
cord-308835-999kewdw	76	33	the	the	DT
cord-308835-999kewdw	76	34	RNA	RNA	NNP
cord-308835-999kewdw	76	35	was	be	VBD
cord-308835-999kewdw	76	36	extracted	extract	VBN
cord-308835-999kewdw	76	37	and	and	CC
cord-308835-999kewdw	76	38	precipitated	precipitate	VBN
cord-308835-999kewdw	76	39	with	with	IN
cord-308835-999kewdw	76	40	ethanol	ethanol	NN
cord-308835-999kewdw	76	41	as	as	IN
cord-308835-999kewdw	76	42	described	describe	VBN
cord-308835-999kewdw	76	43	above	above	RB
cord-308835-999kewdw	76	44	.	.	.
cord-308835-999kewdw	77	1	The	the	DT
cord-308835-999kewdw	77	2	RNA	RNA	NNP
cord-308835-999kewdw	77	3	was	be	VBD
cord-308835-999kewdw	77	4	collected	collect	VBN
cord-308835-999kewdw	77	5	by	by	IN
cord-308835-999kewdw	77	6	centrifugation	centrifugation	NN
cord-308835-999kewdw	77	7	,	,	,
cord-308835-999kewdw	77	8	dried	dry	VBN
cord-308835-999kewdw	77	9	under	under	IN
cord-308835-999kewdw	77	10	a	a	DT
cord-308835-999kewdw	77	11	stream	stream	NN
cord-308835-999kewdw	77	12	of	of	IN
cord-308835-999kewdw	77	13	nitrogen	nitrogen	NN
cord-308835-999kewdw	77	14	,	,	,
cord-308835-999kewdw	77	15	and	and	CC
cord-308835-999kewdw	77	16	dissolved	dissolve	VBD
cord-308835-999kewdw	77	17	in	in	IN
cord-308835-999kewdw	77	18	0.1	0.1	CD
cord-308835-999kewdw	77	19	ml	ml	NNS
cord-308835-999kewdw	77	20	of	of	IN
cord-308835-999kewdw	77	21	SDS	SDS	NNP
cord-308835-999kewdw	77	22	buffer	buffer	NN
cord-308835-999kewdw	77	23	(	(	-LRB-
cord-308835-999kewdw	77	24	0.01	0.01	CD
cord-308835-999kewdw	77	25	M	M	NNP
cord-308835-999kewdw	77	26	Tris	Tris	NNP
cord-308835-999kewdw	77	27	,	,	,
cord-308835-999kewdw	77	28	0.01	0.01	CD
cord-308835-999kewdw	77	29	M	M	NNP
cord-308835-999kewdw	77	30	NaCl	NaCl	NNP
cord-308835-999kewdw	77	31	,	,	,
cord-308835-999kewdw	77	32	0.001	0.001	CD
cord-308835-999kewdw	77	33	M	M	NNP
cord-308835-999kewdw	77	34	EDTA	EDTA	NNP
cord-308835-999kewdw	77	35	,	,	,
cord-308835-999kewdw	77	36	0.1	0.1	CD
cord-308835-999kewdw	77	37	%	%	NN
cord-308835-999kewdw	77	38	SDS	SDS	NNP
cord-308835-999kewdw	77	39	,	,	,
cord-308835-999kewdw	77	40	pH	pH	NNP
cord-308835-999kewdw	77	41	7.4	7.4	CD
cord-308835-999kewdw	77	42	)	)	-RRB-
cord-308835-999kewdw	77	43	.	.	.
cord-308835-999kewdw	78	1	It	-PRON-	PRP
cord-308835-999kewdw	78	2	was	be	VBD
cord-308835-999kewdw	78	3	then	then	RB
cord-308835-999kewdw	78	4	overlaid	overlay	VBN
cord-308835-999kewdw	78	5	on	on	IN
cord-308835-999kewdw	78	6	a	a	DT
cord-308835-999kewdw	78	7	5-ml	5-ml	CD
cord-308835-999kewdw	78	8	lo-30	lo-30	NNP
cord-308835-999kewdw	78	9	%	%	NN
cord-308835-999kewdw	78	10	(	(	-LRB-
cord-308835-999kewdw	78	11	w	w	NNP
cord-308835-999kewdw	78	12	/	/	SYM
cord-308835-999kewdw	78	13	w	w	NN
cord-308835-999kewdw	78	14	)	)	-RRB-
cord-308835-999kewdw	78	15	sucrose	sucrose	NN
cord-308835-999kewdw	78	16	in	in	IN
cord-308835-999kewdw	78	17	SDS	SDS	NNP
cord-308835-999kewdw	78	18	buffer	buffer	NN
cord-308835-999kewdw	78	19	gradient	gradient	NN
cord-308835-999kewdw	78	20	and	and	CC
cord-308835-999kewdw	78	21	centrifuged	centrifuge	VBD
cord-308835-999kewdw	78	22	at	at	IN
cord-308835-999kewdw	78	23	46,000	46,000	CD
cord-308835-999kewdw	78	24	rpm	rpm	NN
cord-308835-999kewdw	78	25	for	for	IN
cord-308835-999kewdw	78	26	107	107	CD
cord-308835-999kewdw	78	27	min	min	NN
cord-308835-999kewdw	78	28	at	at	IN
cord-308835-999kewdw	78	29	20	20	CD
cord-308835-999kewdw	78	30	"	"	``
cord-308835-999kewdw	78	31	in	in	IN
cord-308835-999kewdw	78	32	the	the	DT
cord-308835-999kewdw	78	33	SW50.1	sw50.1	JJ
cord-308835-999kewdw	78	34	rotor	rotor	NN
cord-308835-999kewdw	78	35	.	.	.
cord-308835-999kewdw	79	1	The	the	DT
cord-308835-999kewdw	79	2	gradient	gradient	NN
cord-308835-999kewdw	79	3	was	be	VBD
cord-308835-999kewdw	79	4	fractionated	fractionate	VBN
cord-308835-999kewdw	79	5	and	and	CC
cord-308835-999kewdw	79	6	the	the	DT
cord-308835-999kewdw	79	7	peak	peak	NN
cord-308835-999kewdw	79	8	of	of	IN
cord-308835-999kewdw	79	9	48	48	CD
cord-308835-999kewdw	79	10	S	S	NNP
cord-308835-999kewdw	79	11	virion	virion	NN
cord-308835-999kewdw	79	12	RNA	rna	NN
cord-308835-999kewdw	79	13	(	(	-LRB-
cord-308835-999kewdw	79	14	Robb	Robb	NNP
cord-308835-999kewdw	79	15	and	and	CC
cord-308835-999kewdw	79	16	Bond	Bond	NNP
cord-308835-999kewdw	79	17	,	,	,
cord-308835-999kewdw	79	18	1979b	1979b	CD
cord-308835-999kewdw	79	19	)	)	-RRB-
cord-308835-999kewdw	79	20	located	locate	VBN
cord-308835-999kewdw	79	21	by	by	IN
cord-308835-999kewdw	79	22	counting	count	VBG
cord-308835-999kewdw	79	23	aliquots	aliquot	NNS
cord-308835-999kewdw	79	24	of	of	IN
cord-308835-999kewdw	79	25	each	each	DT
cord-308835-999kewdw	79	26	fraction	fraction	NN
cord-308835-999kewdw	79	27	.	.	.
cord-308835-999kewdw	80	1	These	these	DT
cord-308835-999kewdw	80	2	fractions	fraction	NNS
cord-308835-999kewdw	80	3	were	be	VBD
cord-308835-999kewdw	80	4	pooled	pool	VBN
cord-308835-999kewdw	80	5	,	,	,
cord-308835-999kewdw	80	6	adjusted	adjust	VBN
cord-308835-999kewdw	80	7	to	to	IN
cord-308835-999kewdw	80	8	0.4	0.4	CD
cord-308835-999kewdw	80	9	M	m	NN
cord-308835-999kewdw	80	10	NaCl	nacl	NN
cord-308835-999kewdw	80	11	and	and	CC
cord-308835-999kewdw	80	12	precipitated	precipitate	VBD
cord-308835-999kewdw	80	13	with	with	IN
cord-308835-999kewdw	80	14	ethanol	ethanol	NN
cord-308835-999kewdw	80	15	in	in	IN
cord-308835-999kewdw	80	16	the	the	DT
cord-308835-999kewdw	80	17	presence	presence	NN
cord-308835-999kewdw	80	18	of	of	IN
cord-308835-999kewdw	80	19	100	100	CD
cord-308835-999kewdw	80	20	rg	rg	NNS
cord-308835-999kewdw	80	21	of	of	IN
cord-308835-999kewdw	80	22	tRNA	tRNA	NNP
cord-308835-999kewdw	80	23	carrier	carrier	NN
cord-308835-999kewdw	80	24	.	.	.
cord-308835-999kewdw	81	1	This	this	DT
cord-308835-999kewdw	81	2	material	material	NN
cord-308835-999kewdw	81	3	was	be	VBD
cord-308835-999kewdw	81	4	used	use	VBN
cord-308835-999kewdw	81	5	for	for	IN
cord-308835-999kewdw	81	6	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	81	7	studies	study	NNS
cord-308835-999kewdw	81	8	and	and	CC
cord-308835-999kewdw	81	9	was	be	VBD
cord-308835-999kewdw	81	10	homogeneous	homogeneous	JJ
cord-308835-999kewdw	81	11	upon	upon	IN
cord-308835-999kewdw	81	12	analytical	analytical	JJ
cord-308835-999kewdw	81	13	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	81	14	.	.	.
cord-308835-999kewdw	82	1	Isolation	isolation	NN
cord-308835-999kewdw	82	2	of	of	IN
cord-308835-999kewdw	82	3	poly(A)-containing	poly(A)-containing	NNP
cord-308835-999kewdw	82	4	RNA	RNA	NNP
cord-308835-999kewdw	82	5	.	.	.
cord-308835-999kewdw	83	1	Intracellular	Intracellular	NNP
cord-308835-999kewdw	83	2	RNA	RNA	NNP
cord-308835-999kewdw	83	3	was	be	VBD
cord-308835-999kewdw	83	4	extracted	extract	VBN
cord-308835-999kewdw	83	5	and	and	CC
cord-308835-999kewdw	83	6	precipitated	precipitate	VBN
cord-308835-999kewdw	83	7	with	with	IN
cord-308835-999kewdw	83	8	ethanol	ethanol	NN
cord-308835-999kewdw	83	9	as	as	IN
cord-308835-999kewdw	83	10	described	describe	VBN
cord-308835-999kewdw	83	11	.	.	.
cord-308835-999kewdw	84	1	The	the	DT
cord-308835-999kewdw	84	2	polyadenylated	polyadenylated	JJ
cord-308835-999kewdw	84	3	RNA	RNA	NNP
cord-308835-999kewdw	84	4	species	specie	NNS
cord-308835-999kewdw	84	5	were	be	VBD
cord-308835-999kewdw	84	6	isolated	isolate	VBN
cord-308835-999kewdw	84	7	by	by	IN
cord-308835-999kewdw	84	8	affinity	affinity	NN
cord-308835-999kewdw	84	9	chromatography	chromatography	NN
cord-308835-999kewdw	84	10	over	over	IN
cord-308835-999kewdw	84	11	poly(U	poly(u	$
cord-308835-999kewdw	84	12	)	)	-RRB-
cord-308835-999kewdw	85	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	85	2	as	as	IN
cord-308835-999kewdw	85	3	described	describe	VBN
cord-308835-999kewdw	85	4	by	by	IN
cord-308835-999kewdw	85	5	Wilt	Wilt	NNP
cord-308835-999kewdw	85	6	(	(	-LRB-
cord-308835-999kewdw	85	7	1977	1977	CD
cord-308835-999kewdw	85	8	)	)	-RRB-
cord-308835-999kewdw	85	9	.	.	.
cord-308835-999kewdw	86	1	Agarose	Agarose	NNP
cord-308835-999kewdw	86	2	Gel	Gel	NNP
cord-308835-999kewdw	86	3	Electrophoresis	Electrophoresis	NNP
cord-308835-999kewdw	86	4	1	1	CD
cord-308835-999kewdw	86	5	.	.	.
cord-308835-999kewdw	87	1	Analytical	analytical	JJ
cord-308835-999kewdw	87	2	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	87	3	.	.	.
cord-308835-999kewdw	88	1	Agarose	Agarose	NNP
cord-308835-999kewdw	88	2	gel	gel	NN
cord-308835-999kewdw	88	3	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	88	4	following	follow	VBG
cord-308835-999kewdw	88	5	glyoxal	glyoxal	NNP
cord-308835-999kewdw	88	6	denaturation	denaturation	NNP
cord-308835-999kewdw	88	7	was	be	VBD
cord-308835-999kewdw	88	8	essentially	essentially	RB
cord-308835-999kewdw	88	9	as	as	RB
cord-308835-999kewdw	88	10	previously	previously	RB
cord-308835-999kewdw	88	11	described	describe	VBN
cord-308835-999kewdw	88	12	(	(	-LRB-
cord-308835-999kewdw	88	13	McMaster	McMaster	NNP
cord-308835-999kewdw	88	14	and	and	CC
cord-308835-999kewdw	88	15	Carmichael	Carmichael	NNP
cord-308835-999kewdw	88	16	,	,	,
cord-308835-999kewdw	88	17	1977	1977	CD
cord-308835-999kewdw	88	18	)	)	-RRB-
cord-308835-999kewdw	88	19	.	.	.
cord-308835-999kewdw	89	1	Nucleic	nucleic	JJ
cord-308835-999kewdw	89	2	acids	acid	NNS
cord-308835-999kewdw	89	3	were	be	VBD
cord-308835-999kewdw	89	4	recovered	recover	VBN
cord-308835-999kewdw	89	5	from	from	IN
cord-308835-999kewdw	89	6	ethanol	ethanol	NN
cord-308835-999kewdw	89	7	by	by	IN
cord-308835-999kewdw	89	8	centrifugation	centrifugation	NN
cord-308835-999kewdw	89	9	,	,	,
cord-308835-999kewdw	89	10	dried	dry	VBN
cord-308835-999kewdw	89	11	under	under	IN
cord-308835-999kewdw	89	12	a	a	DT
cord-308835-999kewdw	89	13	stream	stream	NN
cord-308835-999kewdw	89	14	of	of	IN
cord-308835-999kewdw	89	15	nitrogen	nitrogen	NN
cord-308835-999kewdw	89	16	and	and	CC
cord-308835-999kewdw	89	17	dissolved	dissolve	VBN
cord-308835-999kewdw	89	18	in	in	IN
cord-308835-999kewdw	89	19	glyoxal	glyoxal	NNP
cord-308835-999kewdw	89	20	buffer	buffer	NN
cord-308835-999kewdw	89	21	.	.	.
cord-308835-999kewdw	90	1	RNA	RNA	NNP
cord-308835-999kewdw	90	2	or	or	CC
cord-308835-999kewdw	90	3	DNA	DNA	NNP
cord-308835-999kewdw	90	4	was	be	VBD
cord-308835-999kewdw	90	5	reacted	react	VBN
cord-308835-999kewdw	90	6	with	with	IN
cord-308835-999kewdw	90	7	glyoxal	glyoxal	NNP
cord-308835-999kewdw	90	8	at	at	IN
cord-308835-999kewdw	90	9	50	50	CD
cord-308835-999kewdw	90	10	"	"	``
cord-308835-999kewdw	90	11	for	for	IN
cord-308835-999kewdw	90	12	5	5	CD
cord-308835-999kewdw	90	13	min	min	NN
cord-308835-999kewdw	90	14	followed	follow	VBN
cord-308835-999kewdw	90	15	by	by	IN
cord-308835-999kewdw	90	16	an	an	DT
cord-308835-999kewdw	90	17	additional	additional	JJ
cord-308835-999kewdw	90	18	55	55	CD
cord-308835-999kewdw	90	19	min	min	NN
cord-308835-999kewdw	90	20	at	at	IN
cord-308835-999kewdw	90	21	room	room	NN
cord-308835-999kewdw	90	22	temperature	temperature	NN
cord-308835-999kewdw	90	23	.	.	.
cord-308835-999kewdw	91	1	Samples	sample	NNS
cord-308835-999kewdw	91	2	were	be	VBD
cord-308835-999kewdw	91	3	electrophoresed	electrophorese	VBN
cord-308835-999kewdw	91	4	at	at	IN
cord-308835-999kewdw	91	5	100	100	CD
cord-308835-999kewdw	91	6	V	v	NN
cord-308835-999kewdw	91	7	for	for	IN
cord-308835-999kewdw	91	8	4	4	CD
cord-308835-999kewdw	91	9	hr	hr	NN
cord-308835-999kewdw	91	10	in	in	IN
cord-308835-999kewdw	91	11	horizontal	horizontal	JJ
cord-308835-999kewdw	91	12	slab	slab	NN
cord-308835-999kewdw	91	13	gels	gel	NNS
cord-308835-999kewdw	91	14	containing	contain	VBG
cord-308835-999kewdw	91	15	0.7	0.7	CD
cord-308835-999kewdw	91	16	,	,	,
cord-308835-999kewdw	91	17	0.8	0.8	CD
cord-308835-999kewdw	91	18	,	,	,
cord-308835-999kewdw	91	19	or	or	CC
cord-308835-999kewdw	91	20	1.0	1.0	CD
cord-308835-999kewdw	91	21	%	%	NN
cord-308835-999kewdw	91	22	agarose	agarose	VBN
cord-308835-999kewdw	91	23	,	,	,
cord-308835-999kewdw	91	24	0.01	0.01	CD
cord-308835-999kewdw	91	25	M	m	NN
cord-308835-999kewdw	91	26	phosphate	phosphate	NN
cord-308835-999kewdw	91	27	buffer	buffer	NN
cord-308835-999kewdw	91	28	,	,	,
cord-308835-999kewdw	91	29	pH	pH	NNP
cord-308835-999kewdw	91	30	7.0	7.0	CD
cord-308835-999kewdw	91	31	,	,	,
cord-308835-999kewdw	91	32	0.002	0.002	CD
cord-308835-999kewdw	91	33	M	M	NNP
cord-308835-999kewdw	91	34	EDTA	EDTA	NNP
cord-308835-999kewdw	91	35	.	.	.
cord-308835-999kewdw	92	1	When	when	WRB
cord-308835-999kewdw	92	2	appropriate	appropriate	JJ
cord-308835-999kewdw	92	3	,	,	,
cord-308835-999kewdw	92	4	the	the	DT
cord-308835-999kewdw	92	5	gels	gel	NNS
cord-308835-999kewdw	92	6	were	be	VBD
cord-308835-999kewdw	92	7	strained	strain	VBN
cord-308835-999kewdw	92	8	with	with	IN
cord-308835-999kewdw	92	9	acridine	acridine	JJ
cord-308835-999kewdw	92	10	orange	orange	NN
cord-308835-999kewdw	92	11	and	and	CC
cord-308835-999kewdw	92	12	the	the	DT
cord-308835-999kewdw	92	13	positions	position	NNS
cord-308835-999kewdw	92	14	of	of	IN
cord-308835-999kewdw	92	15	rRNA	rRNA	NNP
cord-308835-999kewdw	92	16	were	be	VBD
cord-308835-999kewdw	92	17	marked	mark	VBN
cord-308835-999kewdw	92	18	with	with	IN
cord-308835-999kewdw	92	19	stainless	stainless	JJ
cord-308835-999kewdw	92	20	-	-	HYPH
cord-308835-999kewdw	92	21	steel	steel	NN
cord-308835-999kewdw	92	22	wire	wire	NN
cord-308835-999kewdw	92	23	clips	clip	NNS
cord-308835-999kewdw	92	24	inserted	insert	VBN
cord-308835-999kewdw	92	25	into	into	IN
cord-308835-999kewdw	92	26	the	the	DT
cord-308835-999kewdw	92	27	gel	gel	NN
cord-308835-999kewdw	92	28	.	.	.
cord-308835-999kewdw	93	1	The	the	DT
cord-308835-999kewdw	93	2	gels	gel	NNS
cord-308835-999kewdw	93	3	were	be	VBD
cord-308835-999kewdw	93	4	fixed	fix	VBN
cord-308835-999kewdw	93	5	in	in	IN
cord-308835-999kewdw	93	6	ethanol	ethanol	NN
cord-308835-999kewdw	93	7	,	,	,
cord-308835-999kewdw	93	8	dried	dry	VBN
cord-308835-999kewdw	93	9	under	under	IN
cord-308835-999kewdw	93	10	vacuum	vacuum	NN
cord-308835-999kewdw	93	11	,	,	,
cord-308835-999kewdw	93	12	and	and	CC
cord-308835-999kewdw	93	13	exposed	expose	VBN
cord-308835-999kewdw	93	14	to	to	IN
cord-308835-999kewdw	93	15	Kodak	Kodak	NNP
cord-308835-999kewdw	93	16	XR	XR	NNP
cord-308835-999kewdw	93	17	film	film	NN
cord-308835-999kewdw	93	18	at	at	IN
cord-308835-999kewdw	93	19	room	room	NN
cord-308835-999kewdw	93	20	temperature	temperature	NN
cord-308835-999kewdw	93	21	.	.	.
cord-308835-999kewdw	94	1	To	to	TO
cord-308835-999kewdw	94	2	quantitate	quantitate	VB
cord-308835-999kewdw	94	3	the	the	DT
cord-308835-999kewdw	94	4	individual	individual	JJ
cord-308835-999kewdw	94	5	RNA	RNA	NNP
cord-308835-999kewdw	94	6	species	specie	NNS
cord-308835-999kewdw	94	7	present	present	JJ
cord-308835-999kewdw	94	8	in	in	IN
cord-308835-999kewdw	94	9	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	94	10	extracts	extract	NNS
cord-308835-999kewdw	94	11	two	two	CD
cord-308835-999kewdw	94	12	methods	method	NNS
cord-308835-999kewdw	94	13	were	be	VBD
cord-308835-999kewdw	94	14	used	use	VBN
cord-308835-999kewdw	94	15	.	.	.
cord-308835-999kewdw	95	1	Autoradiographs	Autoradiographs	NNP
cord-308835-999kewdw	95	2	were	be	VBD
cord-308835-999kewdw	95	3	prepared	prepare	VBN
cord-308835-999kewdw	95	4	with	with	IN
cord-308835-999kewdw	95	5	two	two	CD
cord-308835-999kewdw	95	6	exposure	exposure	NN
cord-308835-999kewdw	95	7	times	time	NNS
cord-308835-999kewdw	95	8	to	to	TO
cord-308835-999kewdw	95	9	ensure	ensure	VB
cord-308835-999kewdw	95	10	linearity	linearity	NN
cord-308835-999kewdw	95	11	of	of	IN
cord-308835-999kewdw	95	12	the	the	DT
cord-308835-999kewdw	95	13	film	film	NN
cord-308835-999kewdw	95	14	and	and	CC
cord-308835-999kewdw	95	15	scanned	scan	VBD
cord-308835-999kewdw	95	16	with	with	IN
cord-308835-999kewdw	95	17	a	a	DT
cord-308835-999kewdw	95	18	densitometer	densitometer	NN
cord-308835-999kewdw	95	19	(	(	-LRB-
cord-308835-999kewdw	95	20	E.	E.	NNP
cord-308835-999kewdw	95	21	C.	C.	NNP
cord-308835-999kewdw	95	22	Corporation	Corporation	NNP
cord-308835-999kewdw	95	23	)	)	-RRB-
cord-308835-999kewdw	95	24	.	.	.
cord-308835-999kewdw	96	1	The	the	DT
cord-308835-999kewdw	96	2	area	area	NN
cord-308835-999kewdw	96	3	under	under	IN
cord-308835-999kewdw	96	4	the	the	DT
cord-308835-999kewdw	96	5	peaks	peak	NNS
cord-308835-999kewdw	96	6	was	be	VBD
cord-308835-999kewdw	96	7	quantitated	quantitate	VBN
cord-308835-999kewdw	96	8	by	by	IN
cord-308835-999kewdw	96	9	cutting	cut	VBG
cord-308835-999kewdw	96	10	out	out	RP
cord-308835-999kewdw	96	11	and	and	CC
cord-308835-999kewdw	96	12	weighing	weigh	VBG
cord-308835-999kewdw	96	13	tracings	tracing	NNS
cord-308835-999kewdw	96	14	of	of	IN
cord-308835-999kewdw	96	15	the	the	DT
cord-308835-999kewdw	96	16	scans	scan	NNS
cord-308835-999kewdw	96	17	.	.	.
cord-308835-999kewdw	97	1	Alternatively	alternatively	RB
cord-308835-999kewdw	97	2	,	,	,
cord-308835-999kewdw	97	3	the	the	DT
cord-308835-999kewdw	97	4	regions	region	NNS
cord-308835-999kewdw	97	5	of	of	IN
cord-308835-999kewdw	97	6	the	the	DT
cord-308835-999kewdw	97	7	gel	gel	NN
cord-308835-999kewdw	97	8	corresponding	correspond	VBG
cord-308835-999kewdw	97	9	to	to	IN
cord-308835-999kewdw	97	10	the	the	DT
cord-308835-999kewdw	97	11	bands	band	NNS
cord-308835-999kewdw	97	12	present	present	JJ
cord-308835-999kewdw	97	13	in	in	IN
cord-308835-999kewdw	97	14	the	the	DT
cord-308835-999kewdw	97	15	autoradiographs	autoradiographs	NNP
cord-308835-999kewdw	97	16	were	be	VBD
cord-308835-999kewdw	97	17	excised	excise	VBN
cord-308835-999kewdw	97	18	and	and	CC
cord-308835-999kewdw	97	19	the	the	DT
cord-308835-999kewdw	97	20	amount	amount	NN
cord-308835-999kewdw	97	21	of	of	IN
cord-308835-999kewdw	97	22	RNA	RNA	NNP
cord-308835-999kewdw	97	23	present	present	JJ
cord-308835-999kewdw	97	24	in	in	IN
cord-308835-999kewdw	97	25	the	the	DT
cord-308835-999kewdw	97	26	band	band	NN
cord-308835-999kewdw	97	27	was	be	VBD
cord-308835-999kewdw	97	28	quantitated	quantitate	VBN
cord-308835-999kewdw	97	29	by	by	IN
cord-308835-999kewdw	97	30	liquid	liquid	JJ
cord-308835-999kewdw	97	31	scintillation	scintillation	NN
cord-308835-999kewdw	97	32	counting	counting	NN
cord-308835-999kewdw	97	33	.	.	.
cord-308835-999kewdw	98	1	These	these	DT
cord-308835-999kewdw	98	2	methods	method	NNS
cord-308835-999kewdw	98	3	gave	give	VBD
cord-308835-999kewdw	98	4	similar	similar	JJ
cord-308835-999kewdw	98	5	results	result	NNS
cord-308835-999kewdw	98	6	.	.	.
cord-308835-999kewdw	99	1	Molar	molar	JJ
cord-308835-999kewdw	99	2	ratios	ratio	NNS
cord-308835-999kewdw	99	3	of	of	IN
cord-308835-999kewdw	99	4	RNA	RNA	NNP
cord-308835-999kewdw	99	5	species	specie	NNS
cord-308835-999kewdw	99	6	were	be	VBD
cord-308835-999kewdw	99	7	calculated	calculate	VBN
cord-308835-999kewdw	99	8	by	by	IN
cord-308835-999kewdw	99	9	dividing	divide	VBG
cord-308835-999kewdw	99	10	the	the	DT
cord-308835-999kewdw	99	11	counts	count	NNS
cord-308835-999kewdw	99	12	per	per	IN
cord-308835-999kewdw	99	13	minute	minute	NN
cord-308835-999kewdw	99	14	or	or	CC
cord-308835-999kewdw	99	15	weight	weight	NN
cord-308835-999kewdw	99	16	of	of	IN
cord-308835-999kewdw	99	17	paper	paper	NN
cord-308835-999kewdw	99	18	in	in	IN
cord-308835-999kewdw	99	19	each	each	DT
cord-308835-999kewdw	99	20	peak	peak	NN
cord-308835-999kewdw	99	21	by	by	IN
cord-308835-999kewdw	99	22	its	-PRON-	PRP$
cord-308835-999kewdw	99	23	molecular	molecular	JJ
cord-308835-999kewdw	99	24	weights	weight	NNS
cord-308835-999kewdw	99	25	X	X	NNP
cord-308835-999kewdw	99	26	lop6	lop6	NN
cord-308835-999kewdw	99	27	and	and	CC
cord-308835-999kewdw	99	28	normalizing	normalize	VBG
cord-308835-999kewdw	99	29	all	all	DT
cord-308835-999kewdw	99	30	values	value	NNS
cord-308835-999kewdw	99	31	such	such	JJ
cord-308835-999kewdw	99	32	that	that	IN
cord-308835-999kewdw	99	33	the	the	DT
cord-308835-999kewdw	99	34	major	major	JJ
cord-308835-999kewdw	99	35	RNA	RNA	NNP
cord-308835-999kewdw	99	36	species	specie	NNS
cord-308835-999kewdw	99	37	,	,	,
cord-308835-999kewdw	99	38	RNA7	RNA7	NNP
cord-308835-999kewdw	99	39	(	(	-LRB-
cord-308835-999kewdw	99	40	see	see	VB
cord-308835-999kewdw	99	41	Results	result	NNS
cord-308835-999kewdw	99	42	)	)	-RRB-
cord-308835-999kewdw	99	43	,	,	,
cord-308835-999kewdw	99	44	was	be	VBD
cord-308835-999kewdw	99	45	set	set	VBN
cord-308835-999kewdw	99	46	at	at	IN
cord-308835-999kewdw	99	47	100	100	CD
cord-308835-999kewdw	99	48	.	.	.
cord-308835-999kewdw	100	1	2	2	LS
cord-308835-999kewdw	100	2	.	.	.
cord-308835-999kewdw	101	1	Preparative	preparative	JJ
cord-308835-999kewdw	101	2	electrophwesis	electrophwesis	NN
cord-308835-999kewdw	101	3	.	.	.
cord-308835-999kewdw	102	1	Labeled	label	VBN
cord-308835-999kewdw	102	2	intracellular	intracellular	JJ
cord-308835-999kewdw	102	3	RNA	RNA	NNP
cord-308835-999kewdw	102	4	was	be	VBD
cord-308835-999kewdw	102	5	extracted	extract	VBN
cord-308835-999kewdw	102	6	from	from	IN
cord-308835-999kewdw	102	7	1.2	1.2	CD
cord-308835-999kewdw	102	8	X	x	NN
cord-308835-999kewdw	102	9	lo*-1.6	lo*-1.6	NNP
cord-308835-999kewdw	102	10	X	X	NNP
cord-308835-999kewdw	102	11	10	10	CD
cord-308835-999kewdw	102	12	'	'	POS
cord-308835-999kewdw	102	13	cells	cell	NNS
cord-308835-999kewdw	102	14	,	,	,
cord-308835-999kewdw	102	15	the	the	DT
cord-308835-999kewdw	102	16	poly(A)-containing	poly(A)-containing	NNP
cord-308835-999kewdw	102	17	RNA	RNA	NNP
cord-308835-999kewdw	102	18	species	specie	NNS
cord-308835-999kewdw	102	19	selected	select	VBN
cord-308835-999kewdw	102	20	by	by	IN
cord-308835-999kewdw	102	21	affinity	affinity	NN
cord-308835-999kewdw	102	22	chromatography	chromatography	NN
cord-308835-999kewdw	102	23	,	,	,
cord-308835-999kewdw	102	24	and	and	CC
cord-308835-999kewdw	102	25	then	then	RB
cord-308835-999kewdw	102	26	electrophoresed	electrophorese	VBD
cord-308835-999kewdw	102	27	in	in	IN
cord-308835-999kewdw	102	28	gels	gel	NNS
cord-308835-999kewdw	102	29	containing	contain	VBG
cord-308835-999kewdw	102	30	1	1	CD
cord-308835-999kewdw	102	31	%	%	NN
cord-308835-999kewdw	102	32	low	low	JJ
cord-308835-999kewdw	102	33	melting	melting	NN
cord-308835-999kewdw	102	34	point	point	NN
cord-308835-999kewdw	102	35	agarose	agarose	JJ
cord-308835-999kewdw	102	36	.	.	.
cord-308835-999kewdw	103	1	Two	two	CD
cord-308835-999kewdw	103	2	gel	gel	NN
cord-308835-999kewdw	103	3	systems	system	NNS
cord-308835-999kewdw	103	4	were	be	VBD
cord-308835-999kewdw	103	5	used	use	VBN
cord-308835-999kewdw	103	6	.	.	.
cord-308835-999kewdw	104	1	In	in	IN
cord-308835-999kewdw	104	2	initial	initial	JJ
cord-308835-999kewdw	104	3	experiments	experiment	NNS
cord-308835-999kewdw	104	4	the	the	DT
cord-308835-999kewdw	104	5	phosphate	phosphate	NN
cord-308835-999kewdw	104	6	buffer	buffer	NN
cord-308835-999kewdw	104	7	system	system	NN
cord-308835-999kewdw	104	8	used	use	VBN
cord-308835-999kewdw	104	9	for	for	IN
cord-308835-999kewdw	104	10	analytical	analytical	JJ
cord-308835-999kewdw	104	11	agarose	agarose	NN
cord-308835-999kewdw	104	12	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	104	13	was	be	VBD
cord-308835-999kewdw	104	14	employed	employ	VBN
cord-308835-999kewdw	104	15	without	without	IN
cord-308835-999kewdw	104	16	prior	prior	JJ
cord-308835-999kewdw	104	17	denaturation	denaturation	NN
cord-308835-999kewdw	104	18	of	of	IN
cord-308835-999kewdw	104	19	the	the	DT
cord-308835-999kewdw	104	20	RNA	RNA	NNP
cord-308835-999kewdw	104	21	with	with	IN
cord-308835-999kewdw	104	22	glyoxal	glyoxal	NNP
cord-308835-999kewdw	104	23	and	and	CC
cord-308835-999kewdw	104	24	dimethyl	dimethyl	NNP
cord-308835-999kewdw	104	25	sufoxide	sufoxide	NNP
cord-308835-999kewdw	104	26	(	(	-LRB-
cord-308835-999kewdw	104	27	McMaster	McMaster	NNP
cord-308835-999kewdw	104	28	and	and	CC
cord-308835-999kewdw	104	29	Carmichael	Carmichael	NNP
cord-308835-999kewdw	104	30	,	,	,
cord-308835-999kewdw	104	31	1977	1977	CD
cord-308835-999kewdw	104	32	)	)	-RRB-
cord-308835-999kewdw	104	33	.	.	.
cord-308835-999kewdw	105	1	Electrophoresis	Electrophoresis	NNP
cord-308835-999kewdw	105	2	was	be	VBD
cord-308835-999kewdw	105	3	for	for	IN
cord-308835-999kewdw	105	4	4	4	CD
cord-308835-999kewdw	105	5	hr	hr	NN
cord-308835-999kewdw	105	6	at	at	IN
cord-308835-999kewdw	105	7	100	100	CD
cord-308835-999kewdw	105	8	V.	v.	NN
cord-308835-999kewdw	106	1	The	the	DT
cord-308835-999kewdw	106	2	above	above	JJ
cord-308835-999kewdw	106	3	conditions	condition	NNS
cord-308835-999kewdw	106	4	did	do	VBD
cord-308835-999kewdw	106	5	not	not	RB
cord-308835-999kewdw	106	6	reproducibly	reproducibly	RB
cord-308835-999kewdw	106	7	allow	allow	VB
cord-308835-999kewdw	106	8	complete	complete	JJ
cord-308835-999kewdw	106	9	resolution	resolution	NN
cord-308835-999kewdw	106	10	of	of	IN
cord-308835-999kewdw	106	11	all	all	DT
cord-308835-999kewdw	106	12	virus	virus	NN
cord-308835-999kewdw	106	13	-	-	HYPH
cord-308835-999kewdw	106	14	specific	specific	JJ
cord-308835-999kewdw	106	15	RNA	RNA	NNP
cord-308835-999kewdw	106	16	species	specie	NNS
cord-308835-999kewdw	106	17	.	.	.
cord-308835-999kewdw	107	1	Later	later	JJ
cord-308835-999kewdw	107	2	experiments	experiment	NNS
cord-308835-999kewdw	107	3	employed	employ	VBD
cord-308835-999kewdw	107	4	methyl	methyl	NN
cord-308835-999kewdw	107	5	mercury	mercury	NNP
cord-308835-999kewdw	107	6	de	de	NNP
cord-308835-999kewdw	107	7	-	-	HYPH
cord-308835-999kewdw	107	8	naturation	naturation	NN
cord-308835-999kewdw	107	9	of	of	IN
cord-308835-999kewdw	107	10	the	the	DT
cord-308835-999kewdw	107	11	RNA	RNA	NNP
cord-308835-999kewdw	107	12	and	and	CC
cord-308835-999kewdw	107	13	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	107	14	in	in	IN
cord-308835-999kewdw	107	15	agarose	agarose	JJ
cord-308835-999kewdw	107	16	gels	gel	NNS
cord-308835-999kewdw	107	17	containing	contain	VBG
cord-308835-999kewdw	107	18	5	5	CD
cord-308835-999kewdw	107	19	mM	mm	CD
cord-308835-999kewdw	107	20	methyl	methyl	NN
cord-308835-999kewdw	107	21	mercuric	mercuric	NN
cord-308835-999kewdw	107	22	hydroxide	hydroxide	NN
cord-308835-999kewdw	107	23	(	(	-LRB-
cord-308835-999kewdw	107	24	Bailey	Bailey	NNP
cord-308835-999kewdw	107	25	and	and	CC
cord-308835-999kewdw	107	26	Davidson	Davidson	NNP
cord-308835-999kewdw	107	27	,	,	,
cord-308835-999kewdw	107	28	1976	1976	CD
cord-308835-999kewdw	107	29	)	)	-RRB-
cord-308835-999kewdw	107	30	.	.	.
cord-308835-999kewdw	108	1	Electrophoresis	Electrophoresis	NNP
cord-308835-999kewdw	108	2	was	be	VBD
cord-308835-999kewdw	108	3	at	at	IN
cord-308835-999kewdw	108	4	100	100	CD
cord-308835-999kewdw	108	5	V	v	NN
cord-308835-999kewdw	108	6	for	for	IN
cord-308835-999kewdw	108	7	6	6	CD
cord-308835-999kewdw	108	8	hr	hr	NN
cord-308835-999kewdw	108	9	in	in	IN
cord-308835-999kewdw	108	10	a	a	DT
cord-308835-999kewdw	108	11	horizontal	horizontal	JJ
cord-308835-999kewdw	108	12	1	1	CD
cord-308835-999kewdw	108	13	%	%	NN
cord-308835-999kewdw	108	14	low	low	JJ
cord-308835-999kewdw	108	15	melting	melting	NN
cord-308835-999kewdw	108	16	point	point	NN
cord-308835-999kewdw	108	17	agarose	agarose	VBN
cord-308835-999kewdw	108	18	gel	gel	NN
cord-308835-999kewdw	108	19	.	.	.
cord-308835-999kewdw	109	1	Following	follow	VBG
cord-308835-999kewdw	109	2	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	109	3	,	,	,
cord-308835-999kewdw	109	4	the	the	DT
cord-308835-999kewdw	109	5	majority	majority	NN
cord-308835-999kewdw	109	6	of	of	IN
cord-308835-999kewdw	109	7	the	the	DT
cord-308835-999kewdw	109	8	methyl	methyl	NN
cord-308835-999kewdw	109	9	mercury	mercury	NN
cord-308835-999kewdw	109	10	was	be	VBD
cord-308835-999kewdw	109	11	removed	remove	VBN
cord-308835-999kewdw	109	12	from	from	IN
cord-308835-999kewdw	109	13	the	the	DT
cord-308835-999kewdw	109	14	gel	gel	NN
cord-308835-999kewdw	109	15	by	by	IN
cord-308835-999kewdw	109	16	soaking	soak	VBG
cord-308835-999kewdw	109	17	in	in	RP
cord-308835-999kewdw	109	18	two	two	CD
cord-308835-999kewdw	109	19	changes	change	NNS
cord-308835-999kewdw	109	20	of	of	IN
cord-308835-999kewdw	109	21	750	750	CD
cord-308835-999kewdw	109	22	ml	ml	NN
cord-308835-999kewdw	109	23	sterile	sterile	JJ
cord-308835-999kewdw	109	24	0.5	0.5	CD
cord-308835-999kewdw	109	25	M	m	NN
cord-308835-999kewdw	109	26	ammonium	ammonium	NN
cord-308835-999kewdw	109	27	acetate	acetate	NN
cord-308835-999kewdw	109	28	(	(	-LRB-
cord-308835-999kewdw	109	29	Bailey	Bailey	NNP
cord-308835-999kewdw	109	30	and	and	CC
cord-308835-999kewdw	109	31	Davidson	Davidson	NNP
cord-308835-999kewdw	109	32	,	,	,
cord-308835-999kewdw	109	33	1976	1976	CD
cord-308835-999kewdw	109	34	)	)	-RRB-
cord-308835-999kewdw	109	35	.	.	.
cord-308835-999kewdw	110	1	For	for	IN
cord-308835-999kewdw	110	2	both	both	DT
cord-308835-999kewdw	110	3	gel	gel	NN
cord-308835-999kewdw	110	4	systems	system	NNS
cord-308835-999kewdw	110	5	RNA	RNA	NNP
cord-308835-999kewdw	110	6	species	specie	NNS
cord-308835-999kewdw	110	7	were	be	VBD
cord-308835-999kewdw	110	8	located	locate	VBN
cord-308835-999kewdw	110	9	by	by	IN
cord-308835-999kewdw	110	10	autoradiography	autoradiography	NNP
cord-308835-999kewdw	110	11	of	of	IN
cord-308835-999kewdw	110	12	the	the	DT
cord-308835-999kewdw	110	13	wet	wet	JJ
cord-308835-999kewdw	110	14	gels	gel	NNS
cord-308835-999kewdw	110	15	wrapped	wrap	VBN
cord-308835-999kewdw	110	16	in	in	IN
cord-308835-999kewdw	110	17	Saran	Saran	NNP
cord-308835-999kewdw	110	18	.	.	.
cord-308835-999kewdw	111	1	Agarose	agarose	NN
cord-308835-999kewdw	111	2	strips	strip	NNS
cord-308835-999kewdw	111	3	corresponding	correspond	VBG
cord-308835-999kewdw	111	4	to	to	IN
cord-308835-999kewdw	111	5	the	the	DT
cord-308835-999kewdw	111	6	bands	band	NNS
cord-308835-999kewdw	111	7	seen	see	VBN
cord-308835-999kewdw	111	8	in	in	IN
cord-308835-999kewdw	111	9	the	the	DT
cord-308835-999kewdw	111	10	autoradiographs	autoradiographs	NNP
cord-308835-999kewdw	111	11	were	be	VBD
cord-308835-999kewdw	111	12	cut	cut	VBN
cord-308835-999kewdw	111	13	from	from	IN
cord-308835-999kewdw	111	14	the	the	DT
cord-308835-999kewdw	111	15	gel	gel	NN
cord-308835-999kewdw	111	16	with	with	IN
cord-308835-999kewdw	111	17	a	a	DT
cord-308835-999kewdw	111	18	flamed	flame	VBN
cord-308835-999kewdw	111	19	scalpel	scalpel	NN
cord-308835-999kewdw	111	20	and	and	CC
cord-308835-999kewdw	111	21	placed	place	VBN
cord-308835-999kewdw	111	22	in	in	IN
cord-308835-999kewdw	111	23	tightly	tightly	RB
cord-308835-999kewdw	111	24	capped	cap	VBN
cord-308835-999kewdw	111	25	screw	screw	JJ
cord-308835-999kewdw	111	26	top	top	JJ
cord-308835-999kewdw	111	27	tubes	tube	NNS
cord-308835-999kewdw	111	28	.	.	.
cord-308835-999kewdw	112	1	RNA	RNA	NNP
cord-308835-999kewdw	112	2	was	be	VBD
cord-308835-999kewdw	112	3	recovered	recover	VBN
cord-308835-999kewdw	112	4	from	from	IN
cord-308835-999kewdw	112	5	gels	gel	NNS
cord-308835-999kewdw	112	6	by	by	IN
cord-308835-999kewdw	112	7	melting	melt	VBG
cord-308835-999kewdw	112	8	the	the	DT
cord-308835-999kewdw	112	9	agarose	agarose	NN
cord-308835-999kewdw	112	10	at	at	IN
cord-308835-999kewdw	112	11	70	70	CD
cord-308835-999kewdw	112	12	"	"	''
cord-308835-999kewdw	112	13	for	for	IN
cord-308835-999kewdw	112	14	5	5	CD
cord-308835-999kewdw	112	15	min	min	NN
cord-308835-999kewdw	112	16	.	.	.
cord-308835-999kewdw	113	1	Five	five	CD
cord-308835-999kewdw	113	2	milliliters	milliliter	NNS
cord-308835-999kewdw	113	3	of	of	IN
cord-308835-999kewdw	113	4	a	a	DT
cord-308835-999kewdw	113	5	slurry	slurry	NN
cord-308835-999kewdw	113	6	of	of	IN
cord-308835-999kewdw	113	7	HA	HA	NNP
cord-308835-999kewdw	113	8	,	,	,
cord-308835-999kewdw	113	9	prewarmed	prewarme	VBN
cord-308835-999kewdw	113	10	to	to	IN
cord-308835-999kewdw	113	11	37	37	CD
cord-308835-999kewdw	113	12	"	"	''
cord-308835-999kewdw	113	13	,	,	,
cord-308835-999kewdw	113	14	was	be	VBD
cord-308835-999kewdw	113	15	added	add	VBN
cord-308835-999kewdw	113	16	to	to	IN
cord-308835-999kewdw	113	17	each	each	DT
cord-308835-999kewdw	113	18	tube	tube	NN
cord-308835-999kewdw	113	19	.	.	.
cord-308835-999kewdw	114	1	Samples	sample	NNS
cord-308835-999kewdw	114	2	containing	contain	VBG
cord-308835-999kewdw	114	3	methyl	methyl	NN
cord-308835-999kewdw	114	4	mercury	mercury	NN
cord-308835-999kewdw	114	5	had	have	VBD
cord-308835-999kewdw	114	6	2-mercaptoethanol	2-mercaptoethanol	CD
cord-308835-999kewdw	114	7	added	add	VBN
cord-308835-999kewdw	114	8	to	to	IN
cord-308835-999kewdw	114	9	a	a	DT
cord-308835-999kewdw	114	10	concentration	concentration	NN
cord-308835-999kewdw	114	11	of	of	IN
cord-308835-999kewdw	114	12	1	1	CD
cord-308835-999kewdw	114	13	%	%	NN
cord-308835-999kewdw	114	14	.	.	.
cord-308835-999kewdw	115	1	The	the	DT
cord-308835-999kewdw	115	2	mixture	mixture	NN
cord-308835-999kewdw	115	3	was	be	VBD
cord-308835-999kewdw	115	4	cooled	cool	VBN
cord-308835-999kewdw	115	5	to	to	IN
cord-308835-999kewdw	115	6	37	37	CD
cord-308835-999kewdw	115	7	"	"	''
cord-308835-999kewdw	115	8	,	,	,
cord-308835-999kewdw	115	9	HA	HA	NNP
cord-308835-999kewdw	115	10	was	be	VBD
cord-308835-999kewdw	115	11	collected	collect	VBN
cord-308835-999kewdw	115	12	by	by	IN
cord-308835-999kewdw	115	13	centrifugation	centrifugation	NN
cord-308835-999kewdw	115	14	,	,	,
cord-308835-999kewdw	115	15	and	and	CC
cord-308835-999kewdw	115	16	washed	wash	VBD
cord-308835-999kewdw	115	17	twice	twice	RB
cord-308835-999kewdw	115	18	with	with	IN
cord-308835-999kewdw	115	19	Tris	Tris	NNP
cord-308835-999kewdw	115	20	acetate	acetate	NN
cord-308835-999kewdw	115	21	buffer	buffer	NN
cord-308835-999kewdw	115	22	(	(	-LRB-
cord-308835-999kewdw	115	23	10	10	CD
cord-308835-999kewdw	115	24	mM	mM	NNP
cord-308835-999kewdw	115	25	Tris	Tris	NNP
cord-308835-999kewdw	115	26	,	,	,
cord-308835-999kewdw	115	27	20	20	CD
cord-308835-999kewdw	115	28	mM	mm	CD
cord-308835-999kewdw	115	29	sodium	sodium	NN
cord-308835-999kewdw	115	30	acetate	acetate	NN
cord-308835-999kewdw	115	31	,	,	,
cord-308835-999kewdw	115	32	5	5	CD
cord-308835-999kewdw	115	33	mM	mm	CD
cord-308835-999kewdw	115	34	EDTA	EDTA	NNP
cord-308835-999kewdw	115	35	,	,	,
cord-308835-999kewdw	115	36	pH	pH	NNP
cord-308835-999kewdw	115	37	7.3	7.3	CD
cord-308835-999kewdw	115	38	)	)	-RRB-
cord-308835-999kewdw	115	39	in	in	IN
cord-308835-999kewdw	115	40	a	a	DT
cord-308835-999kewdw	115	41	37	37	CD
cord-308835-999kewdw	115	42	"	"	``
cord-308835-999kewdw	115	43	warm	warm	JJ
cord-308835-999kewdw	115	44	room	room	NN
cord-308835-999kewdw	115	45	to	to	TO
cord-308835-999kewdw	115	46	remove	remove	VB
cord-308835-999kewdw	115	47	the	the	DT
cord-308835-999kewdw	115	48	liquified	liquified	JJ
cord-308835-999kewdw	115	49	agarose	agarose	NN
cord-308835-999kewdw	115	50	.	.	.
cord-308835-999kewdw	116	1	For	for	IN
cord-308835-999kewdw	116	2	samples	sample	NNS
cord-308835-999kewdw	116	3	which	which	WDT
cord-308835-999kewdw	116	4	had	have	VBD
cord-308835-999kewdw	116	5	been	be	VBN
cord-308835-999kewdw	116	6	treated	treat	VBN
cord-308835-999kewdw	116	7	with	with	IN
cord-308835-999kewdw	116	8	methyl	methyl	NN
cord-308835-999kewdw	116	9	mercury	mercury	NN
cord-308835-999kewdw	116	10	,	,	,
cord-308835-999kewdw	116	11	the	the	DT
cord-308835-999kewdw	116	12	Tris	Tris	NNP
cord-308835-999kewdw	116	13	acetate	acetate	NN
cord-308835-999kewdw	116	14	buffer	buffer	NN
cord-308835-999kewdw	116	15	was	be	VBD
cord-308835-999kewdw	116	16	supplemented	supplement	VBN
cord-308835-999kewdw	116	17	with	with	IN
cord-308835-999kewdw	116	18	1	1	CD
cord-308835-999kewdw	116	19	%	%	NN
cord-308835-999kewdw	116	20	2-mercaptoethanol	2-mercaptoethanol	CD
cord-308835-999kewdw	116	21	.	.	.
cord-308835-999kewdw	117	1	The	the	DT
cord-308835-999kewdw	117	2	HA	HA	NNP
cord-308835-999kewdw	117	3	was	be	VBD
cord-308835-999kewdw	117	4	resuspended	resuspend	VBN
cord-308835-999kewdw	117	5	in	in	IN
cord-308835-999kewdw	117	6	5	5	CD
cord-308835-999kewdw	117	7	ml	ml	NNS
cord-308835-999kewdw	117	8	of	of	IN
cord-308835-999kewdw	117	9	Tris	Tris	NNP
cord-308835-999kewdw	117	10	acetate	acetate	NN
cord-308835-999kewdw	117	11	buffer	buffer	NN
cord-308835-999kewdw	117	12	and	and	CC
cord-308835-999kewdw	117	13	transferred	transfer	VBN
cord-308835-999kewdw	117	14	to	to	IN
cord-308835-999kewdw	117	15	ethanolwashed	ethanolwashe	VBN
cord-308835-999kewdw	117	16	disposable	disposable	JJ
cord-308835-999kewdw	117	17	columns	column	NNS
cord-308835-999kewdw	117	18	(	(	-LRB-
cord-308835-999kewdw	117	19	Quick	Quick	NNP
cord-308835-999kewdw	117	20	-	-	HYPH
cord-308835-999kewdw	117	21	sep	sep	NNP
cord-308835-999kewdw	117	22	,	,	,
cord-308835-999kewdw	117	23	&	&	CC
cord-308835-999kewdw	117	24	S	S	NNP
cord-308835-999kewdw	117	25	-	-	HYPH
cord-308835-999kewdw	117	26	Q	Q	NNP
cord-308835-999kewdw	117	27	obtained	obtain	VBD
cord-308835-999kewdw	117	28	from	from	IN
cord-308835-999kewdw	117	29	Isolab	Isolab	NNP
cord-308835-999kewdw	117	30	)	)	-RRB-
cord-308835-999kewdw	117	31	placed	place	VBN
cord-308835-999kewdw	117	32	in	in	IN
cord-308835-999kewdw	117	33	heat	heat	NN
cord-308835-999kewdw	117	34	sterilized	sterilize	VBN
cord-308835-999kewdw	117	35	16	16	CD
cord-308835-999kewdw	117	36	X	x	NN
cord-308835-999kewdw	117	37	125-mm	125-mm	CD
cord-308835-999kewdw	117	38	culture	culture	NN
cord-308835-999kewdw	117	39	tubes	tube	NNS
cord-308835-999kewdw	117	40	.	.	.
cord-308835-999kewdw	118	1	The	the	DT
cord-308835-999kewdw	118	2	culture	culture	NN
cord-308835-999kewdw	118	3	tubes	tube	NNS
cord-308835-999kewdw	118	4	containing	contain	VBG
cord-308835-999kewdw	118	5	the	the	DT
cord-308835-999kewdw	118	6	columns	column	NNS
cord-308835-999kewdw	118	7	were	be	VBD
cord-308835-999kewdw	118	8	centrifuged	centrifuge	VBN
cord-308835-999kewdw	118	9	at	at	IN
cord-308835-999kewdw	118	10	1000	1000	CD
cord-308835-999kewdw	118	11	Q	q	NN
cord-308835-999kewdw	118	12	for	for	IN
cord-308835-999kewdw	118	13	10	10	CD
cord-308835-999kewdw	118	14	min	min	NN
cord-308835-999kewdw	118	15	at	at	IN
cord-308835-999kewdw	118	16	room	room	NN
cord-308835-999kewdw	118	17	temperature	temperature	NN
cord-308835-999kewdw	118	18	to	to	TO
cord-308835-999kewdw	118	19	remove	remove	VB
cord-308835-999kewdw	118	20	the	the	DT
cord-308835-999kewdw	118	21	Tris	Tris	NNP
cord-308835-999kewdw	118	22	acetate	acetate	NN
cord-308835-999kewdw	118	23	buffer	buffer	NN
cord-308835-999kewdw	118	24	.	.	.
cord-308835-999kewdw	119	1	The	the	DT
cord-308835-999kewdw	119	2	RNA	RNA	NNP
cord-308835-999kewdw	119	3	was	be	VBD
cord-308835-999kewdw	119	4	eluted	elute	VBN
cord-308835-999kewdw	119	5	from	from	IN
cord-308835-999kewdw	119	6	HA	HA	NNP
cord-308835-999kewdw	119	7	columns	column	NNS
cord-308835-999kewdw	119	8	by	by	IN
cord-308835-999kewdw	119	9	two	two	CD
cord-308835-999kewdw	119	10	washes	wash	NNS
cord-308835-999kewdw	119	11	of	of	IN
cord-308835-999kewdw	119	12	1.0	1.0	CD
cord-308835-999kewdw	119	13	ml	ml	NNS
cord-308835-999kewdw	119	14	of	of	IN
cord-308835-999kewdw	119	15	0.4	0.4	CD
cord-308835-999kewdw	119	16	M	m	NN
cord-308835-999kewdw	119	17	sodium	sodium	NN
cord-308835-999kewdw	119	18	phosphate	phosphate	NN
cord-308835-999kewdw	119	19	,	,	,
cord-308835-999kewdw	119	20	1	1	CD
cord-308835-999kewdw	119	21	mM	mm	CD
cord-308835-999kewdw	119	22	EDTA	EDTA	NNP
cord-308835-999kewdw	119	23	(	(	-LRB-
cord-308835-999kewdw	119	24	pH	pH	NNP
cord-308835-999kewdw	119	25	7.0	7.0	CD
cord-308835-999kewdw	119	26	)	)	-RRB-
cord-308835-999kewdw	119	27	which	which	WDT
cord-308835-999kewdw	119	28	were	be	VBD
cord-308835-999kewdw	119	29	similarly	similarly	RB
cord-308835-999kewdw	119	30	forced	force	VBN
cord-308835-999kewdw	119	31	from	from	IN
cord-308835-999kewdw	119	32	the	the	DT
cord-308835-999kewdw	119	33	columns	column	NNS
cord-308835-999kewdw	119	34	by	by	IN
cord-308835-999kewdw	119	35	centrifugation	centrifugation	NN
cord-308835-999kewdw	119	36	at	at	IN
cord-308835-999kewdw	119	37	1OOOg	1ooog	CD
cord-308835-999kewdw	119	38	for	for	IN
cord-308835-999kewdw	119	39	10	10	CD
cord-308835-999kewdw	119	40	min	min	NN
cord-308835-999kewdw	119	41	.	.	.
cord-308835-999kewdw	120	1	The	the	DT
cord-308835-999kewdw	120	2	phosphate	phosphate	NN
cord-308835-999kewdw	120	3	eluates	eluate	NNS
cord-308835-999kewdw	120	4	were	be	VBD
cord-308835-999kewdw	120	5	pooled	pool	VBN
cord-308835-999kewdw	120	6	and	and	CC
cord-308835-999kewdw	120	7	the	the	DT
cord-308835-999kewdw	120	8	RNA	RNA	NNP
cord-308835-999kewdw	120	9	precipitated	precipitate	VBD
cord-308835-999kewdw	120	10	with	with	IN
cord-308835-999kewdw	120	11	CTAB	CTAB	NNP
cord-308835-999kewdw	120	12	as	as	IN
cord-308835-999kewdw	120	13	described	describe	VBN
cord-308835-999kewdw	120	14	by	by	IN
cord-308835-999kewdw	120	15	Stern	Stern	NNP
cord-308835-999kewdw	120	16	and	and	CC
cord-308835-999kewdw	120	17	Kennedy	Kennedy	NNP
cord-308835-999kewdw	120	18	(	(	-LRB-
cord-308835-999kewdw	120	19	1980a	1980a	CD
cord-308835-999kewdw	120	20	)	)	-RRB-
cord-308835-999kewdw	120	21	.	.	.
cord-308835-999kewdw	121	1	The	the	DT
cord-308835-999kewdw	121	2	CTAB	CTAB	NNP
cord-308835-999kewdw	121	3	precipitates	precipitate	NNS
cord-308835-999kewdw	121	4	were	be	VBD
cord-308835-999kewdw	121	5	dissolved	dissolve	VBN
cord-308835-999kewdw	121	6	in	in	IN
cord-308835-999kewdw	121	7	0.2	0.2	CD
cord-308835-999kewdw	121	8	ml	ml	NNS
cord-308835-999kewdw	121	9	of	of	IN
cord-308835-999kewdw	121	10	50	50	CD
cord-308835-999kewdw	121	11	mM	mM	NNP
cord-308835-999kewdw	121	12	Tris	Tris	NNP
cord-308835-999kewdw	121	13	,	,	,
cord-308835-999kewdw	121	14	pH	pH	NNP
cord-308835-999kewdw	121	15	7.4	7.4	CD
cord-308835-999kewdw	121	16	,	,	,
cord-308835-999kewdw	121	17	containing	contain	VBG
cord-308835-999kewdw	121	18	1	1	CD
cord-308835-999kewdw	121	19	M	M	NNP
cord-308835-999kewdw	121	20	NaCl	nacl	NN
cord-308835-999kewdw	121	21	and	and	CC
cord-308835-999kewdw	121	22	1	1	CD
cord-308835-999kewdw	121	23	mM	mm	CD
cord-308835-999kewdw	121	24	EDTA	EDTA	NNP
cord-308835-999kewdw	121	25	and	and	CC
cord-308835-999kewdw	121	26	the	the	DT
cord-308835-999kewdw	121	27	RNA	RNA	NNP
cord-308835-999kewdw	121	28	precipitated	precipitate	VBD
cord-308835-999kewdw	121	29	with	with	IN
cord-308835-999kewdw	121	30	1.0	1.0	CD
cord-308835-999kewdw	121	31	ml	ml	NNS
cord-308835-999kewdw	121	32	of	of	IN
cord-308835-999kewdw	121	33	ethanol	ethanol	NN
cord-308835-999kewdw	121	34	.	.	.
cord-308835-999kewdw	122	1	Ribonuclease	Ribonuclease	NNP
cord-308835-999kewdw	122	2	Tl	Tl	NNP
cord-308835-999kewdw	122	3	$	$	$
cord-308835-999kewdw	122	4	nger;orinting	nger;orinting	NN
cord-308835-999kewdw	122	5	.	.	.
cord-308835-999kewdw	123	1	RNA	RNA	NNP
cord-308835-999kewdw	123	2	was	be	VBD
cord-308835-999kewdw	123	3	recovered	recover	VBN
cord-308835-999kewdw	123	4	from	from	IN
cord-308835-999kewdw	123	5	ethanol	ethanol	NN
cord-308835-999kewdw	123	6	by	by	IN
cord-308835-999kewdw	123	7	centrifugation	centrifugation	NN
cord-308835-999kewdw	123	8	,	,	,
cord-308835-999kewdw	123	9	dissolved	dissolve	VBN
cord-308835-999kewdw	123	10	in	in	IN
cord-308835-999kewdw	123	11	0.1	0.1	CD
cord-308835-999kewdw	123	12	ml	ml	NNS
cord-308835-999kewdw	123	13	of	of	IN
cord-308835-999kewdw	123	14	sterile	sterile	JJ
cord-308835-999kewdw	123	15	water	water	NN
cord-308835-999kewdw	123	16	,	,	,
cord-308835-999kewdw	123	17	transferred	transfer	VBN
cord-308835-999kewdw	123	18	to	to	IN
cord-308835-999kewdw	123	19	a	a	DT
cord-308835-999kewdw	123	20	sterile	sterile	JJ
cord-308835-999kewdw	123	21	1.5-ml	1.5-ml	CD
cord-308835-999kewdw	123	22	Eppendorf	Eppendorf	NNP
cord-308835-999kewdw	123	23	centrifuge	centrifuge	NN
cord-308835-999kewdw	123	24	tube	tube	NN
cord-308835-999kewdw	123	25	,	,	,
cord-308835-999kewdw	123	26	and	and	CC
cord-308835-999kewdw	123	27	the	the	DT
cord-308835-999kewdw	123	28	water	water	NN
cord-308835-999kewdw	123	29	was	be	VBD
cord-308835-999kewdw	123	30	evaporated	evaporate	VBN
cord-308835-999kewdw	123	31	with	with	IN
cord-308835-999kewdw	123	32	a	a	DT
cord-308835-999kewdw	123	33	stream	stream	NN
cord-308835-999kewdw	123	34	of	of	IN
cord-308835-999kewdw	123	35	nitrogen	nitrogen	NN
cord-308835-999kewdw	123	36	.	.	.
cord-308835-999kewdw	124	1	The	the	DT
cord-308835-999kewdw	124	2	RNA	RNA	NNP
cord-308835-999kewdw	124	3	was	be	VBD
cord-308835-999kewdw	124	4	digested	digest	VBN
cord-308835-999kewdw	124	5	with	with	IN
cord-308835-999kewdw	124	6	10	10	CD
cord-308835-999kewdw	124	7	~1	~1	CD
cord-308835-999kewdw	124	8	of	of	IN
cord-308835-999kewdw	124	9	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	124	10	T1	T1	NNP
cord-308835-999kewdw	124	11	(	(	-LRB-
cord-308835-999kewdw	124	12	1	1	CD
cord-308835-999kewdw	124	13	mg	mg	NNP
cord-308835-999kewdw	124	14	/	/	SYM
cord-308835-999kewdw	124	15	ml	ml	NNP
cord-308835-999kewdw	124	16	)	)	-RRB-
cord-308835-999kewdw	124	17	in	in	IN
cord-308835-999kewdw	124	18	10	10	CD
cord-308835-999kewdw	124	19	mM	mm	CD
cord-308835-999kewdw	124	20	Tris	Tris	NNP
cord-308835-999kewdw	124	21	,	,	,
cord-308835-999kewdw	124	22	pH	pH	NNP
cord-308835-999kewdw	124	23	7.6	7.6	CD
cord-308835-999kewdw	124	24	,	,	,
cord-308835-999kewdw	124	25	at	at	IN
cord-308835-999kewdw	124	26	37	37	CD
cord-308835-999kewdw	124	27	"	"	``
cord-308835-999kewdw	124	28	for	for	IN
cord-308835-999kewdw	124	29	30	30	CD
cord-308835-999kewdw	124	30	min	min	NN
cord-308835-999kewdw	124	31	.	.	.
cord-308835-999kewdw	125	1	Ten	ten	CD
cord-308835-999kewdw	125	2	microliters	microliter	NNS
cord-308835-999kewdw	125	3	of	of	IN
cord-308835-999kewdw	125	4	a	a	DT
cord-308835-999kewdw	125	5	solution	solution	NN
cord-308835-999kewdw	125	6	containing	contain	VBG
cord-308835-999kewdw	125	7	5	5	CD
cord-308835-999kewdw	125	8	M	M	NNP
cord-308835-999kewdw	125	9	urea	urea	NN
cord-308835-999kewdw	125	10	,	,	,
cord-308835-999kewdw	125	11	50	50	CD
cord-308835-999kewdw	125	12	%	%	NN
cord-308835-999kewdw	125	13	sucrose	sucrose	NN
cord-308835-999kewdw	125	14	,	,	,
cord-308835-999kewdw	125	15	0.1	0.1	CD
cord-308835-999kewdw	125	16	%	%	NN
cord-308835-999kewdw	125	17	bromphenol	bromphenol	NN
cord-308835-999kewdw	125	18	blue	blue	JJ
cord-308835-999kewdw	125	19	,	,	,
cord-308835-999kewdw	125	20	and	and	CC
cord-308835-999kewdw	125	21	0.1	0.1	CD
cord-308835-999kewdw	125	22	%	%	NN
cord-308835-999kewdw	125	23	xylene	xylene	NN
cord-308835-999kewdw	125	24	cyan01	cyan01	NNS
cord-308835-999kewdw	125	25	FF	FF	NNP
cord-308835-999kewdw	125	26	was	be	VBD
cord-308835-999kewdw	125	27	added	add	VBN
cord-308835-999kewdw	125	28	to	to	IN
cord-308835-999kewdw	125	29	the	the	DT
cord-308835-999kewdw	125	30	digestion	digestion	NN
cord-308835-999kewdw	125	31	products	product	NNS
cord-308835-999kewdw	125	32	.	.	.
cord-308835-999kewdw	126	1	The	the	DT
cord-308835-999kewdw	126	2	RNase	RNase	NNP
cord-308835-999kewdw	126	3	T1-resistent	T1-resistent	NNP
cord-308835-999kewdw	126	4	oligonucleoties	oligonucleotie	NNS
cord-308835-999kewdw	126	5	were	be	VBD
cord-308835-999kewdw	126	6	separated	separate	VBN
cord-308835-999kewdw	126	7	by	by	IN
cord-308835-999kewdw	126	8	two	two	CD
cord-308835-999kewdw	126	9	-	-	HYPH
cord-308835-999kewdw	126	10	dimensional	dimensional	JJ
cord-308835-999kewdw	126	11	polyacrylamide	polyacrylamide	NN
cord-308835-999kewdw	126	12	gel	gel	NN
cord-308835-999kewdw	126	13	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	126	14	as	as	IN
cord-308835-999kewdw	126	15	described	describe	VBN
cord-308835-999kewdw	126	16	by	by	IN
cord-308835-999kewdw	126	17	Stern	Stern	NNP
cord-308835-999kewdw	126	18	and	and	CC
cord-308835-999kewdw	126	19	Kennedy	Kennedy	NNP
cord-308835-999kewdw	126	20	(	(	-LRB-
cord-308835-999kewdw	126	21	1980a	1980a	CD
cord-308835-999kewdw	126	22	)	)	-RRB-
cord-308835-999kewdw	126	23	with	with	IN
cord-308835-999kewdw	126	24	the	the	DT
cord-308835-999kewdw	126	25	following	following	JJ
cord-308835-999kewdw	126	26	modification	modification	NN
cord-308835-999kewdw	126	27	.	.	.
cord-308835-999kewdw	127	1	After	after	IN
cord-308835-999kewdw	127	2	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	127	3	in	in	IN
cord-308835-999kewdw	127	4	the	the	DT
cord-308835-999kewdw	127	5	first	first	JJ
cord-308835-999kewdw	127	6	dimension	dimension	NN
cord-308835-999kewdw	127	7	the	the	DT
cord-308835-999kewdw	127	8	gel	gel	NN
cord-308835-999kewdw	127	9	strips	strip	NNS
cord-308835-999kewdw	127	10	were	be	VBD
cord-308835-999kewdw	127	11	washed	wash	VBN
cord-308835-999kewdw	127	12	for	for	IN
cord-308835-999kewdw	127	13	25	25	CD
cord-308835-999kewdw	127	14	min	min	NN
cord-308835-999kewdw	127	15	with	with	IN
cord-308835-999kewdw	127	16	two	two	CD
cord-308835-999kewdw	127	17	changes	change	NNS
cord-308835-999kewdw	127	18	of	of	IN
cord-308835-999kewdw	127	19	100	100	CD
cord-308835-999kewdw	127	20	mM	mm	CD
cord-308835-999kewdw	127	21	Tris	Tris	NNP
cord-308835-999kewdw	127	22	borate	borate	NN
cord-308835-999kewdw	127	23	buffer	buffer	NN
cord-308835-999kewdw	127	24	containing	contain	VBG
cord-308835-999kewdw	127	25	2.5	2.5	CD
cord-308835-999kewdw	127	26	mM	mm	CD
cord-308835-999kewdw	127	27	EDTA	EDTA	NNP
cord-308835-999kewdw	127	28	,	,	,
cord-308835-999kewdw	127	29	pH	pH	NNP
cord-308835-999kewdw	127	30	8.3	8.3	CD
cord-308835-999kewdw	127	31	,	,	,
cord-308835-999kewdw	127	32	prior	prior	RB
cord-308835-999kewdw	127	33	to	to	IN
cord-308835-999kewdw	127	34	pouring	pour	VBG
cord-308835-999kewdw	127	35	the	the	DT
cord-308835-999kewdw	127	36	second	second	JJ
cord-308835-999kewdw	127	37	dimension	dimension	NN
cord-308835-999kewdw	127	38	gel	gel	NN
cord-308835-999kewdw	127	39	(	(	-LRB-
cord-308835-999kewdw	127	40	Lee	Lee	NNP
cord-308835-999kewdw	127	41	et	et	NNP
cord-308835-999kewdw	127	42	al	al	NNP
cord-308835-999kewdw	127	43	.	.	NNP
cord-308835-999kewdw	127	44	,	,	,
cord-308835-999kewdw	127	45	1979	1979	CD
cord-308835-999kewdw	127	46	)	)	-RRB-
cord-308835-999kewdw	127	47	.	.	.
cord-308835-999kewdw	128	1	Following	follow	VBG
cord-308835-999kewdw	128	2	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	128	3	the	the	DT
cord-308835-999kewdw	128	4	gels	gel	NNS
cord-308835-999kewdw	128	5	were	be	VBD
cord-308835-999kewdw	128	6	wrapped	wrap	VBN
cord-308835-999kewdw	128	7	with	with	IN
cord-308835-999kewdw	128	8	polyethylene	polyethylene	NN
cord-308835-999kewdw	128	9	sheets	sheet	NNS
cord-308835-999kewdw	128	10	and	and	CC
cord-308835-999kewdw	128	11	exposed	expose	VBN
cord-308835-999kewdw	128	12	at	at	IN
cord-308835-999kewdw	128	13	-70	-70	NN
cord-308835-999kewdw	128	14	"	"	''
cord-308835-999kewdw	128	15	to	to	IN
cord-308835-999kewdw	128	16	Kodak	Kodak	NNP
cord-308835-999kewdw	128	17	XR	XR	NNP
cord-308835-999kewdw	128	18	film	film	NN
cord-308835-999kewdw	128	19	in	in	IN
cord-308835-999kewdw	128	20	the	the	DT
cord-308835-999kewdw	128	21	presence	presence	NN
cord-308835-999kewdw	128	22	of	of	IN
cord-308835-999kewdw	128	23	Cronex	Cronex	NNP
cord-308835-999kewdw	128	24	lightning	lightning	NN
cord-308835-999kewdw	128	25	plus	plus	CC
cord-308835-999kewdw	128	26	(	(	-LRB-
cord-308835-999kewdw	128	27	Du	Du	NNP
cord-308835-999kewdw	128	28	-	-	HYPH
cord-308835-999kewdw	128	29	Pont	Pont	NNP
cord-308835-999kewdw	128	30	)	)	-RRB-
cord-308835-999kewdw	128	31	intensifying	intensify	VBG
cord-308835-999kewdw	128	32	screens	screen	NNS
cord-308835-999kewdw	128	33	(	(	-LRB-
cord-308835-999kewdw	128	34	Laskey	Laskey	NNP
cord-308835-999kewdw	128	35	and	and	CC
cord-308835-999kewdw	128	36	Mills	Mills	NNPS
cord-308835-999kewdw	128	37	,	,	,
cord-308835-999kewdw	128	38	1977	1977	CD
cord-308835-999kewdw	128	39	)	)	-RRB-
cord-308835-999kewdw	128	40	.	.	.
cord-308835-999kewdw	129	1	Exposures	exposure	NNS
cord-308835-999kewdw	129	2	ranged	range	VBD
cord-308835-999kewdw	129	3	from	from	IN
cord-308835-999kewdw	129	4	2	2	CD
cord-308835-999kewdw	129	5	days	day	NNS
cord-308835-999kewdw	129	6	to	to	IN
cord-308835-999kewdw	129	7	3	3	CD
cord-308835-999kewdw	129	8	weeks	week	NNS
cord-308835-999kewdw	129	9	.	.	.
cord-308835-999kewdw	130	1	Preparation	preparation	NN
cord-308835-999kewdw	130	2	of	of	IN
cord-308835-999kewdw	130	3	molecular	molecular	JJ
cord-308835-999kewdw	130	4	weight	weight	NN
cord-308835-999kewdw	130	5	markers	marker	NNS
cord-308835-999kewdw	130	6	.	.	.
cord-308835-999kewdw	131	1	Mengovirus	Mengovirus	NNP
cord-308835-999kewdw	131	2	was	be	VBD
cord-308835-999kewdw	131	3	purified	purify	VBN
cord-308835-999kewdw	131	4	from	from	IN
cord-308835-999kewdw	131	5	infected	infected	JJ
cord-308835-999kewdw	131	6	17CL	17cl	CD
cord-308835-999kewdw	131	7	.	.	.
cord-308835-999kewdw	132	1	1	1	CD
cord-308835-999kewdw	132	2	cells	cell	NNS
cord-308835-999kewdw	132	3	labeled	label	VBN
cord-308835-999kewdw	132	4	with	with	IN
cord-308835-999kewdw	132	5	[	[	-LRB-
cord-308835-999kewdw	132	6	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	132	7	from	from	IN
cord-308835-999kewdw	132	8	4	4	CD
cord-308835-999kewdw	132	9	to	to	IN
cord-308835-999kewdw	132	10	16	16	CD
cord-308835-999kewdw	132	11	hpi	hpi	NNP
cord-308835-999kewdw	132	12	.	.	.
cord-308835-999kewdw	133	1	The	the	DT
cord-308835-999kewdw	133	2	culture	culture	NN
cord-308835-999kewdw	133	3	fluids	fluid	NNS
cord-308835-999kewdw	133	4	were	be	VBD
cord-308835-999kewdw	133	5	clarified	clarify	VBN
cord-308835-999kewdw	133	6	by	by	IN
cord-308835-999kewdw	133	7	centrifugation	centrifugation	NN
cord-308835-999kewdw	133	8	at	at	IN
cord-308835-999kewdw	133	9	10,000	10,000	CD
cord-308835-999kewdw	133	10	g	g	NN
cord-308835-999kewdw	133	11	for	for	IN
cord-308835-999kewdw	133	12	30	30	CD
cord-308835-999kewdw	133	13	min	min	NN
cord-308835-999kewdw	133	14	.	.	.
cord-308835-999kewdw	134	1	Sodium	sodium	NN
cord-308835-999kewdw	134	2	dodecyl	dodecyl	NNP
cord-308835-999kewdw	134	3	sulfate	sulfate	NN
cord-308835-999kewdw	134	4	was	be	VBD
cord-308835-999kewdw	134	5	added	add	VBN
cord-308835-999kewdw	134	6	to	to	IN
cord-308835-999kewdw	134	7	the	the	DT
cord-308835-999kewdw	134	8	clarified	clarify	VBN
cord-308835-999kewdw	134	9	fluids	fluid	NNS
cord-308835-999kewdw	134	10	to	to	IN
cord-308835-999kewdw	134	11	a	a	DT
cord-308835-999kewdw	134	12	concentration	concentration	NN
cord-308835-999kewdw	134	13	of	of	IN
cord-308835-999kewdw	134	14	0.1	0.1	CD
cord-308835-999kewdw	134	15	%	%	NN
cord-308835-999kewdw	134	16	and	and	CC
cord-308835-999kewdw	134	17	the	the	DT
cord-308835-999kewdw	134	18	virus	virus	NN
cord-308835-999kewdw	134	19	pelleted	pellete	VBN
cord-308835-999kewdw	134	20	through	through	IN
cord-308835-999kewdw	134	21	a	a	DT
cord-308835-999kewdw	134	22	1.5-ml	1.5-ml	CD
cord-308835-999kewdw	134	23	30	30	CD
cord-308835-999kewdw	134	24	%	%	NN
cord-308835-999kewdw	134	25	SUclose	SUclose	NNP
cord-308835-999kewdw	134	26	pad	pad	NN
cord-308835-999kewdw	134	27	by	by	IN
cord-308835-999kewdw	134	28	centrifugation	centrifugation	NN
cord-308835-999kewdw	134	29	at	at	IN
cord-308835-999kewdw	134	30	25,000	25,000	CD
cord-308835-999kewdw	134	31	rpm	rpm	NN
cord-308835-999kewdw	134	32	in	in	IN
cord-308835-999kewdw	134	33	the	the	DT
cord-308835-999kewdw	134	34	SW	SW	NNP
cord-308835-999kewdw	134	35	27.1	27.1	CD
cord-308835-999kewdw	134	36	rotor	rotor	NN
cord-308835-999kewdw	134	37	for	for	IN
cord-308835-999kewdw	134	38	3	3	CD
cord-308835-999kewdw	134	39	hr	hr	NN
cord-308835-999kewdw	134	40	at	at	IN
cord-308835-999kewdw	134	41	20	20	CD
cord-308835-999kewdw	134	42	"	"	''
cord-308835-999kewdw	134	43	.	.	.
cord-308835-999kewdw	135	1	The	the	DT
cord-308835-999kewdw	135	2	virus	virus	NN
cord-308835-999kewdw	135	3	was	be	VBD
cord-308835-999kewdw	135	4	resuspended	resuspend	VBN
cord-308835-999kewdw	135	5	in	in	IN
cord-308835-999kewdw	135	6	1	1	CD
cord-308835-999kewdw	135	7	ml	ml	NNS
cord-308835-999kewdw	135	8	of	of	IN
cord-308835-999kewdw	135	9	0.01	0.01	CD
cord-308835-999kewdw	135	10	M	M	NNP
cord-308835-999kewdw	135	11	Tris	Tris	NNP
cord-308835-999kewdw	135	12	buffer	buffer	NN
cord-308835-999kewdw	135	13	,	,	,
cord-308835-999kewdw	135	14	pH	pH	NNP
cord-308835-999kewdw	135	15	7.4	7.4	CD
cord-308835-999kewdw	135	16	,	,	,
cord-308835-999kewdw	135	17	layered	layer	VBN
cord-308835-999kewdw	135	18	onto	onto	IN
cord-308835-999kewdw	135	19	a	a	DT
cord-308835-999kewdw	135	20	16-ml	16-ml	CD
cord-308835-999kewdw	135	21	cesium	cesium	NN
cord-308835-999kewdw	135	22	chloride	chloride	NN
cord-308835-999kewdw	135	23	gradient	gradient	NN
cord-308835-999kewdw	135	24	(	(	-LRB-
cord-308835-999kewdw	135	25	1.2	1.2	CD
cord-308835-999kewdw	135	26	-	-	SYM
cord-308835-999kewdw	135	27	1.4	1.4	CD
cord-308835-999kewdw	135	28	g	g	NN
cord-308835-999kewdw	135	29	/	/	SYM
cord-308835-999kewdw	135	30	cm3	cm3	NNP
cord-308835-999kewdw	135	31	)	)	-RRB-
cord-308835-999kewdw	135	32	and	and	CC
cord-308835-999kewdw	135	33	centrifuged	centrifuge	VBN
cord-308835-999kewdw	135	34	overnight	overnight	RB
cord-308835-999kewdw	135	35	in	in	IN
cord-308835-999kewdw	135	36	the	the	DT
cord-308835-999kewdw	135	37	SW27.1	sw27.1	JJ
cord-308835-999kewdw	135	38	rotor	rotor	NN
cord-308835-999kewdw	135	39	at	at	IN
cord-308835-999kewdw	135	40	24,000	24,000	CD
cord-308835-999kewdw	135	41	rpm	rpm	NN
cord-308835-999kewdw	135	42	.	.	.
cord-308835-999kewdw	136	1	The	the	DT
cord-308835-999kewdw	136	2	gradient	gradient	NN
cord-308835-999kewdw	136	3	was	be	VBD
cord-308835-999kewdw	136	4	fractionated	fractionate	VBN
cord-308835-999kewdw	136	5	,	,	,
cord-308835-999kewdw	136	6	aliquots	aliquot	NNS
cord-308835-999kewdw	136	7	were	be	VBD
cord-308835-999kewdw	136	8	counted	count	VBN
cord-308835-999kewdw	136	9	and	and	CC
cord-308835-999kewdw	136	10	the	the	DT
cord-308835-999kewdw	136	11	peak	peak	NN
cord-308835-999kewdw	136	12	of	of	IN
cord-308835-999kewdw	136	13	radioactive	radioactive	JJ
cord-308835-999kewdw	136	14	virus	virus	NN
cord-308835-999kewdw	136	15	at	at	IN
cord-308835-999kewdw	136	16	a	a	DT
cord-308835-999kewdw	136	17	density	density	NN
cord-308835-999kewdw	136	18	of	of	IN
cord-308835-999kewdw	136	19	1.34	1.34	CD
cord-308835-999kewdw	136	20	g	g	NN
cord-308835-999kewdw	136	21	/	/	SYM
cord-308835-999kewdw	136	22	cm3	cm3	NNP
cord-308835-999kewdw	136	23	was	be	VBD
cord-308835-999kewdw	136	24	pooled	pool	VBN
cord-308835-999kewdw	136	25	.	.	.
cord-308835-999kewdw	137	1	This	this	DT
cord-308835-999kewdw	137	2	material	material	NN
cord-308835-999kewdw	137	3	was	be	VBD
cord-308835-999kewdw	137	4	diluted	dilute	VBN
cord-308835-999kewdw	137	5	with	with	IN
cord-308835-999kewdw	137	6	0.01	0.01	CD
cord-308835-999kewdw	137	7	M	M	NNP
cord-308835-999kewdw	137	8	Tris	Tris	NNP
cord-308835-999kewdw	137	9	,	,	,
cord-308835-999kewdw	137	10	pH	pH	NNP
cord-308835-999kewdw	137	11	7.4	7.4	CD
cord-308835-999kewdw	137	12	,	,	,
cord-308835-999kewdw	137	13	and	and	CC
cord-308835-999kewdw	137	14	pelleted	pellete	VBN
cord-308835-999kewdw	137	15	in	in	IN
cord-308835-999kewdw	137	16	the	the	DT
cord-308835-999kewdw	137	17	SW50.1	sw50.1	JJ
cord-308835-999kewdw	137	18	rotor	rotor	NN
cord-308835-999kewdw	137	19	at	at	IN
cord-308835-999kewdw	137	20	45,000	45,000	CD
cord-308835-999kewdw	137	21	rpm	rpm	NN
cord-308835-999kewdw	137	22	for	for	IN
cord-308835-999kewdw	137	23	1	1	CD
cord-308835-999kewdw	137	24	hr	hr	NN
cord-308835-999kewdw	137	25	.	.	.
cord-308835-999kewdw	138	1	The	the	DT
cord-308835-999kewdw	138	2	pelleted	pelleted	JJ
cord-308835-999kewdw	138	3	virus	virus	NN
cord-308835-999kewdw	138	4	was	be	VBD
cord-308835-999kewdw	138	5	resuspended	resuspend	VBN
cord-308835-999kewdw	138	6	in	in	IN
cord-308835-999kewdw	138	7	0.01	0.01	CD
cord-308835-999kewdw	138	8	M	M	NNP
cord-308835-999kewdw	138	9	Tris	Tris	NNP
cord-308835-999kewdw	138	10	buffer	buffer	NN
cord-308835-999kewdw	138	11	,	,	,
cord-308835-999kewdw	138	12	the	the	DT
cord-308835-999kewdw	138	13	RNA	RNA	NNP
cord-308835-999kewdw	138	14	deproteinized	deproteinize	VBD
cord-308835-999kewdw	138	15	with	with	IN
cord-308835-999kewdw	138	16	SDS	SDS	NNP
cord-308835-999kewdw	138	17	and	and	CC
cord-308835-999kewdw	138	18	proteinase	proteinase	NN
cord-308835-999kewdw	138	19	K	K	NNP
cord-308835-999kewdw	138	20	,	,	,
cord-308835-999kewdw	138	21	extracted	extract	VBN
cord-308835-999kewdw	138	22	with	with	IN
cord-308835-999kewdw	138	23	phenol	phenol	NN
cord-308835-999kewdw	138	24	chloroform	chloroform	NN
cord-308835-999kewdw	138	25	,	,	,
cord-308835-999kewdw	138	26	and	and	CC
cord-308835-999kewdw	138	27	precipitated	precipitate	VBN
cord-308835-999kewdw	138	28	with	with	IN
cord-308835-999kewdw	138	29	ethanol	ethanol	NN
cord-308835-999kewdw	138	30	.	.	.
cord-308835-999kewdw	139	1	The	the	DT
cord-308835-999kewdw	139	2	RNA	RNA	NNP
cord-308835-999kewdw	139	3	was	be	VBD
cord-308835-999kewdw	139	4	collected	collect	VBN
cord-308835-999kewdw	139	5	by	by	IN
cord-308835-999kewdw	139	6	centrifugation	centrifugation	NN
cord-308835-999kewdw	139	7	and	and	CC
cord-308835-999kewdw	139	8	prepared	prepare	VBN
cord-308835-999kewdw	139	9	for	for	IN
cord-308835-999kewdw	139	10	analytical	analytical	JJ
cord-308835-999kewdw	139	11	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	139	12	.	.	.
cord-308835-999kewdw	140	1	VSV	VSV	NNP
cord-308835-999kewdw	140	2	RNA	RNA	NNP
cord-308835-999kewdw	140	3	was	be	VBD
cord-308835-999kewdw	140	4	prepared	prepare	VBN
cord-308835-999kewdw	140	5	from	from	IN
cord-308835-999kewdw	140	6	VSVinfected	VSVinfected	NNP
cord-308835-999kewdw	140	7	BHK	BHK	NNP
cord-308835-999kewdw	140	8	21	21	CD
cord-308835-999kewdw	140	9	cells	cell	NNS
cord-308835-999kewdw	140	10	labeled	label	VBN
cord-308835-999kewdw	140	11	with	with	IN
cord-308835-999kewdw	140	12	[	[	-LRB-
cord-308835-999kewdw	140	13	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	140	14	from	from	IN
cord-308835-999kewdw	140	15	2	2	CD
cord-308835-999kewdw	140	16	to	to	IN
cord-308835-999kewdw	140	17	16	16	CD
cord-308835-999kewdw	140	18	hpi	hpi	NNP
cord-308835-999kewdw	140	19	.	.	.
cord-308835-999kewdw	141	1	Culture	culture	NN
cord-308835-999kewdw	141	2	fluids	fluid	NNS
cord-308835-999kewdw	141	3	were	be	VBD
cord-308835-999kewdw	141	4	clarified	clarify	VBN
cord-308835-999kewdw	141	5	by	by	IN
cord-308835-999kewdw	141	6	centrifugation	centrifugation	NN
cord-308835-999kewdw	141	7	at	at	IN
cord-308835-999kewdw	141	8	10,000	10,000	CD
cord-308835-999kewdw	141	9	g	g	NN
cord-308835-999kewdw	141	10	for	for	IN
cord-308835-999kewdw	141	11	30	30	CD
cord-308835-999kewdw	141	12	min	min	NN
cord-308835-999kewdw	141	13	.	.	.
cord-308835-999kewdw	142	1	VSV	VSV	NNP
cord-308835-999kewdw	142	2	was	be	VBD
cord-308835-999kewdw	142	3	pelleted	pellete	VBN
cord-308835-999kewdw	142	4	from	from	IN
cord-308835-999kewdw	142	5	the	the	DT
cord-308835-999kewdw	142	6	clarified	clarify	VBN
cord-308835-999kewdw	142	7	supernate	supernate	NNP
cord-308835-999kewdw	142	8	by	by	IN
cord-308835-999kewdw	142	9	centrifuging	centrifuge	VBG
cord-308835-999kewdw	142	10	in	in	IN
cord-308835-999kewdw	142	11	the	the	DT
cord-308835-999kewdw	142	12	SW40	SW40	NNP
cord-308835-999kewdw	142	13	rotor	rotor	NN
cord-308835-999kewdw	142	14	at	at	IN
cord-308835-999kewdw	142	15	37,000	37,000	CD
cord-308835-999kewdw	142	16	rpm	rpm	NN
cord-308835-999kewdw	142	17	for	for	IN
cord-308835-999kewdw	142	18	1	1	CD
cord-308835-999kewdw	142	19	hr	hr	NN
cord-308835-999kewdw	142	20	,	,	,
cord-308835-999kewdw	142	21	the	the	DT
cord-308835-999kewdw	142	22	virus	virus	NN
cord-308835-999kewdw	142	23	pellet	pellet	NN
cord-308835-999kewdw	142	24	was	be	VBD
cord-308835-999kewdw	142	25	taken	take	VBN
cord-308835-999kewdw	142	26	up	up	RP
cord-308835-999kewdw	142	27	in	in	IN
cord-308835-999kewdw	142	28	0.01	0.01	CD
cord-308835-999kewdw	142	29	M	M	NNP
cord-308835-999kewdw	142	30	Tris	Tris	NNP
cord-308835-999kewdw	142	31	buffer	buffer	NN
cord-308835-999kewdw	142	32	,	,	,
cord-308835-999kewdw	142	33	pH	pH	NNP
cord-308835-999kewdw	142	34	7.4	7.4	CD
cord-308835-999kewdw	142	35	,	,	,
cord-308835-999kewdw	143	1	the	the	DT
cord-308835-999kewdw	143	2	RNA	RNA	NNP
cord-308835-999kewdw	143	3	deproteinized	deproteinize	VBD
cord-308835-999kewdw	143	4	with	with	IN
cord-308835-999kewdw	143	5	SDS	SDS	NNP
cord-308835-999kewdw	143	6	and	and	CC
cord-308835-999kewdw	143	7	proteinase	proteinase	NN
cord-308835-999kewdw	143	8	K	K	NNP
cord-308835-999kewdw	143	9	,	,	,
cord-308835-999kewdw	143	10	and	and	CC
cord-308835-999kewdw	143	11	precipitated	precipitate	VBN
cord-308835-999kewdw	143	12	with	with	IN
cord-308835-999kewdw	143	13	ethanol	ethanol	NN
cord-308835-999kewdw	143	14	.	.	.
cord-308835-999kewdw	144	1	This	this	DT
cord-308835-999kewdw	144	2	VSV	VSV	NNP
cord-308835-999kewdw	144	3	RNA	RNA	NNP
cord-308835-999kewdw	144	4	preparation	preparation	NN
cord-308835-999kewdw	144	5	was	be	VBD
cord-308835-999kewdw	144	6	used	use	VBN
cord-308835-999kewdw	144	7	as	as	IN
cord-308835-999kewdw	144	8	a	a	DT
cord-308835-999kewdw	144	9	molecular	molecular	JJ
cord-308835-999kewdw	144	10	weight	weight	NN
cord-308835-999kewdw	144	11	marker	marker	NN
cord-308835-999kewdw	144	12	without	without	IN
cord-308835-999kewdw	144	13	further	further	JJ
cord-308835-999kewdw	144	14	purification	purification	NN
cord-308835-999kewdw	144	15	although	although	IN
cord-308835-999kewdw	144	16	it	-PRON-	PRP
cord-308835-999kewdw	144	17	was	be	VBD
cord-308835-999kewdw	144	18	contaminated	contaminate	VBN
cord-308835-999kewdw	144	19	with	with	IN
cord-308835-999kewdw	144	20	significant	significant	JJ
cord-308835-999kewdw	144	21	amounts	amount	NNS
cord-308835-999kewdw	144	22	of	of	IN
cord-308835-999kewdw	144	23	28	28	CD
cord-308835-999kewdw	144	24	S	S	NNP
cord-308835-999kewdw	144	25	and	and	CC
cord-308835-999kewdw	144	26	18	18	CD
cord-308835-999kewdw	144	27	S	S	NNP
cord-308835-999kewdw	144	28	RNA	RNA	NNP
cord-308835-999kewdw	144	29	.	.	.
cord-308835-999kewdw	145	1	HeLa	HeLa	NNP
cord-308835-999kewdw	145	2	cells	cell	NNS
cord-308835-999kewdw	145	3	were	be	VBD
cord-308835-999kewdw	145	4	infected	infect	VBN
cord-308835-999kewdw	145	5	with	with	IN
cord-308835-999kewdw	145	6	Ad2	ad2	CD
cord-308835-999kewdw	145	7	and	and	CC
cord-308835-999kewdw	145	8	labeled	label	VBN
cord-308835-999kewdw	145	9	with	with	IN
cord-308835-999kewdw	145	10	[	[	-LRB-
cord-308835-999kewdw	145	11	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	145	12	from	from	IN
cord-308835-999kewdw	145	13	16	16	CD
cord-308835-999kewdw	145	14	to	to	TO
cord-308835-999kewdw	145	15	40	40	CD
cord-308835-999kewdw	145	16	hpi	hpi	NNP
cord-308835-999kewdw	145	17	.	.	.
cord-308835-999kewdw	146	1	Infected	infected	JJ
cord-308835-999kewdw	146	2	cells	cell	NNS
cord-308835-999kewdw	146	3	were	be	VBD
cord-308835-999kewdw	146	4	scraped	scrape	VBN
cord-308835-999kewdw	146	5	from	from	IN
cord-308835-999kewdw	146	6	the	the	DT
cord-308835-999kewdw	146	7	substrate	substrate	NN
cord-308835-999kewdw	146	8	,	,	,
cord-308835-999kewdw	146	9	pelleted	pellete	VBN
cord-308835-999kewdw	146	10	by	by	IN
cord-308835-999kewdw	146	11	centrifugation	centrifugation	NN
cord-308835-999kewdw	146	12	,	,	,
cord-308835-999kewdw	146	13	and	and	CC
cord-308835-999kewdw	146	14	resuspended	resuspend	VBD
cord-308835-999kewdw	146	15	in	in	IN
cord-308835-999kewdw	146	16	1	1	CD
cord-308835-999kewdw	146	17	ml	ml	NNS
cord-308835-999kewdw	146	18	of	of	IN
cord-308835-999kewdw	146	19	0.01	0.01	CD
cord-308835-999kewdw	146	20	M	M	NNP
cord-308835-999kewdw	146	21	Tris	Tris	NNP
cord-308835-999kewdw	146	22	,	,	,
cord-308835-999kewdw	146	23	pH	pH	NNP
cord-308835-999kewdw	146	24	8.1	8.1	CD
cord-308835-999kewdw	146	25	.	.	.
cord-308835-999kewdw	147	1	The	the	DT
cord-308835-999kewdw	147	2	cells	cell	NNS
cord-308835-999kewdw	147	3	were	be	VBD
cord-308835-999kewdw	147	4	disrupted	disrupt	VBN
cord-308835-999kewdw	147	5	by	by	IN
cord-308835-999kewdw	147	6	sonication	sonication	NN
cord-308835-999kewdw	147	7	and	and	CC
cord-308835-999kewdw	147	8	the	the	DT
cord-308835-999kewdw	147	9	labeled	label	VBN
cord-308835-999kewdw	147	10	virus	virus	NN
cord-308835-999kewdw	147	11	was	be	VBD
cord-308835-999kewdw	147	12	purified	purify	VBN
cord-308835-999kewdw	147	13	as	as	IN
cord-308835-999kewdw	147	14	previously	previously	RB
cord-308835-999kewdw	147	15	described	describe	VBN
cord-308835-999kewdw	147	16	(	(	-LRB-
cord-308835-999kewdw	147	17	Horwitz	Horwitz	NNP
cord-308835-999kewdw	147	18	,	,	,
cord-308835-999kewdw	147	19	1971	1971	CD
cord-308835-999kewdw	147	20	)	)	-RRB-
cord-308835-999kewdw	147	21	.	.	.
cord-308835-999kewdw	148	1	The	the	DT
cord-308835-999kewdw	148	2	purified	purify	VBN
cord-308835-999kewdw	148	3	virus	virus	NN
cord-308835-999kewdw	148	4	was	be	VBD
cord-308835-999kewdw	148	5	diluted	dilute	VBN
cord-308835-999kewdw	148	6	with	with	IN
cord-308835-999kewdw	148	7	0.01	0.01	CD
cord-308835-999kewdw	148	8	M	M	NNP
cord-308835-999kewdw	148	9	Tris	Tris	NNP
cord-308835-999kewdw	148	10	,	,	,
cord-308835-999kewdw	148	11	pH	pH	NNP
cord-308835-999kewdw	148	12	8.1	8.1	CD
cord-308835-999kewdw	148	13	,	,	,
cord-308835-999kewdw	148	14	and	and	CC
cord-308835-999kewdw	148	15	pelleted	pellete	VBN
cord-308835-999kewdw	148	16	by	by	IN
cord-308835-999kewdw	148	17	centrifugation	centrifugation	NN
cord-308835-999kewdw	148	18	at	at	IN
cord-308835-999kewdw	148	19	37,000	37,000	CD
cord-308835-999kewdw	148	20	rpm	rpm	NN
cord-308835-999kewdw	148	21	for	for	IN
cord-308835-999kewdw	148	22	1	1	CD
cord-308835-999kewdw	148	23	hr	hr	NN
cord-308835-999kewdw	148	24	.	.	.
cord-308835-999kewdw	149	1	The	the	DT
cord-308835-999kewdw	149	2	virus	virus	NN
cord-308835-999kewdw	149	3	was	be	VBD
cord-308835-999kewdw	149	4	resuspended	resuspend	VBN
cord-308835-999kewdw	149	5	in	in	IN
cord-308835-999kewdw	149	6	1	1	CD
cord-308835-999kewdw	149	7	ml	ml	NNS
cord-308835-999kewdw	149	8	of	of	IN
cord-308835-999kewdw	149	9	0.01	0.01	CD
cord-308835-999kewdw	149	10	M	M	NNP
cord-308835-999kewdw	149	11	Tris	Tris	NNP
cord-308835-999kewdw	149	12	buffer	buffer	NN
cord-308835-999kewdw	149	13	(	(	-LRB-
cord-308835-999kewdw	149	14	pH	pH	NNP
cord-308835-999kewdw	149	15	7.4	7.4	CD
cord-308835-999kewdw	149	16	)	)	-RRB-
cord-308835-999kewdw	149	17	,	,	,
cord-308835-999kewdw	149	18	extracted	extract	VBN
cord-308835-999kewdw	149	19	with	with	IN
cord-308835-999kewdw	149	20	phenol	phenol	NN
cord-308835-999kewdw	149	21	(	(	-LRB-
cord-308835-999kewdw	149	22	Petterson	Petterson	NNP
cord-308835-999kewdw	149	23	and	and	CC
cord-308835-999kewdw	149	24	Sambrook	Sambrook	NNP
cord-308835-999kewdw	149	25	,	,	,
cord-308835-999kewdw	149	26	1973	1973	CD
cord-308835-999kewdw	149	27	)	)	-RRB-
cord-308835-999kewdw	149	28	,	,	,
cord-308835-999kewdw	149	29	and	and	CC
cord-308835-999kewdw	149	30	the	the	DT
cord-308835-999kewdw	149	31	DNA	dna	NN
cord-308835-999kewdw	149	32	precipitated	precipitate	VBN
cord-308835-999kewdw	149	33	with	with	IN
cord-308835-999kewdw	149	34	ethanol	ethanol	NN
cord-308835-999kewdw	149	35	.	.	.
cord-308835-999kewdw	150	1	The	the	DT
cord-308835-999kewdw	150	2	DNA	dna	NN
cord-308835-999kewdw	150	3	was	be	VBD
cord-308835-999kewdw	150	4	recovered	recover	VBN
cord-308835-999kewdw	150	5	from	from	IN
cord-308835-999kewdw	150	6	ethanol	ethanol	NN
cord-308835-999kewdw	150	7	by	by	IN
cord-308835-999kewdw	150	8	centrifugation	centrifugation	NN
cord-308835-999kewdw	150	9	and	and	CC
cord-308835-999kewdw	150	10	denatured	denature	VBN
cord-308835-999kewdw	150	11	with	with	IN
cord-308835-999kewdw	150	12	glyoxal	glyoxal	NN
cord-308835-999kewdw	150	13	prior	prior	RB
cord-308835-999kewdw	150	14	to	to	IN
cord-308835-999kewdw	150	15	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	150	16	.	.	.
cord-308835-999kewdw	151	1	Ribosomal	Ribosomal	NNP
cord-308835-999kewdw	151	2	RNA	RNA	NNP
cord-308835-999kewdw	151	3	was	be	VBD
cord-308835-999kewdw	151	4	purified	purify	VBN
cord-308835-999kewdw	151	5	from	from	IN
cord-308835-999kewdw	151	6	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	151	7	extracts	extract	NNS
cord-308835-999kewdw	151	8	of	of	IN
cord-308835-999kewdw	151	9	17CL	17cl	CD
cord-308835-999kewdw	151	10	.	.	.
cord-308835-999kewdw	152	1	1	1	CD
cord-308835-999kewdw	152	2	or	or	CC
cord-308835-999kewdw	152	3	BHK-21	BHK-21	NNP
cord-308835-999kewdw	152	4	cells	cell	NNS
cord-308835-999kewdw	152	5	which	which	WDT
cord-308835-999kewdw	152	6	were	be	VBD
cord-308835-999kewdw	152	7	labeled	label	VBN
cord-308835-999kewdw	152	8	for	for	IN
cord-308835-999kewdw	152	9	24	24	CD
cord-308835-999kewdw	152	10	hr	hr	NN
cord-308835-999kewdw	152	11	with	with	IN
cord-308835-999kewdw	152	12	[	[	-LRB-
cord-308835-999kewdw	152	13	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	152	14	.	.	.
cord-308835-999kewdw	153	1	SDS	SDS	NNP
cord-308835-999kewdw	153	2	was	be	VBD
cord-308835-999kewdw	153	3	added	add	VBN
cord-308835-999kewdw	153	4	to	to	IN
cord-308835-999kewdw	153	5	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	153	6	extracts	extract	NNS
cord-308835-999kewdw	153	7	to	to	IN
cord-308835-999kewdw	153	8	a	a	DT
cord-308835-999kewdw	153	9	final	final	JJ
cord-308835-999kewdw	153	10	concentration	concentration	NN
cord-308835-999kewdw	153	11	of	of	IN
cord-308835-999kewdw	153	12	1	1	CD
cord-308835-999kewdw	153	13	%	%	NN
cord-308835-999kewdw	153	14	and	and	CC
cord-308835-999kewdw	153	15	the	the	DT
cord-308835-999kewdw	153	16	18	18	CD
cord-308835-999kewdw	153	17	and	and	CC
cord-308835-999kewdw	153	18	28	28	CD
cord-308835-999kewdw	153	19	S	S	NNP
cord-308835-999kewdw	153	20	rRNA	rRNA	NNS
cord-308835-999kewdw	153	21	were	be	VBD
cord-308835-999kewdw	153	22	purified	purify	VBN
cord-308835-999kewdw	153	23	by	by	IN
cord-308835-999kewdw	153	24	centrifugation	centrifugation	NN
cord-308835-999kewdw	153	25	through	through	IN
cord-308835-999kewdw	153	26	5	5	CD
cord-308835-999kewdw	153	27	-	-	SYM
cord-308835-999kewdw	153	28	30	30	CD
cord-308835-999kewdw	153	29	%	%	NN
cord-308835-999kewdw	154	1	(	(	-LRB-
cord-308835-999kewdw	154	2	w	w	NNP
cord-308835-999kewdw	154	3	/	/	SYM
cord-308835-999kewdw	154	4	w	w	NN
cord-308835-999kewdw	154	5	)	)	-RRB-
cord-308835-999kewdw	154	6	sucrose	sucrose	NN
cord-308835-999kewdw	154	7	gradients	gradient	NNS
cord-308835-999kewdw	154	8	(	(	-LRB-
cord-308835-999kewdw	154	9	containing	contain	VBG
cord-308835-999kewdw	154	10	0.01	0.01	CD
cord-308835-999kewdw	154	11	M	M	NNP
cord-308835-999kewdw	154	12	Tris	Tris	NNP
cord-308835-999kewdw	154	13	,	,	,
cord-308835-999kewdw	154	14	0.01	0.01	CD
cord-308835-999kewdw	154	15	M	M	NNP
cord-308835-999kewdw	154	16	NaCl	NaCl	NNP
cord-308835-999kewdw	154	17	,	,	,
cord-308835-999kewdw	154	18	0.001	0.001	CD
cord-308835-999kewdw	154	19	M	M	NNP
cord-308835-999kewdw	154	20	EDTA	EDTA	NNP
cord-308835-999kewdw	154	21	,	,	,
cord-308835-999kewdw	154	22	0.1	0.1	CD
cord-308835-999kewdw	154	23	%	%	NN
cord-308835-999kewdw	154	24	SDS	SDS	NNP
cord-308835-999kewdw	154	25	,	,	,
cord-308835-999kewdw	154	26	pH	pH	NNP
cord-308835-999kewdw	154	27	7.4	7.4	CD
cord-308835-999kewdw	154	28	)	)	-RRB-
cord-308835-999kewdw	154	29	at	at	IN
cord-308835-999kewdw	154	30	37,000	37,000	CD
cord-308835-999kewdw	154	31	rpm	rpm	NN
cord-308835-999kewdw	154	32	for	for	IN
cord-308835-999kewdw	154	33	4	4	CD
cord-308835-999kewdw	154	34	hr	hr	NN
cord-308835-999kewdw	154	35	in	in	IN
cord-308835-999kewdw	154	36	the	the	DT
cord-308835-999kewdw	154	37	SW40	SW40	NNP
cord-308835-999kewdw	154	38	rotor	rotor	NN
cord-308835-999kewdw	154	39	at	at	IN
cord-308835-999kewdw	154	40	20	20	CD
cord-308835-999kewdw	154	41	"	"	''
cord-308835-999kewdw	154	42	.	.	.
cord-308835-999kewdw	155	1	The	the	DT
cord-308835-999kewdw	155	2	28	28	CD
cord-308835-999kewdw	155	3	and	and	CC
cord-308835-999kewdw	155	4	18	18	CD
cord-308835-999kewdw	155	5	S	s	NN
cord-308835-999kewdw	155	6	peaks	peak	NNS
cord-308835-999kewdw	155	7	were	be	VBD
cord-308835-999kewdw	155	8	located	locate	VBN
cord-308835-999kewdw	155	9	by	by	IN
cord-308835-999kewdw	155	10	counting	count	VBG
cord-308835-999kewdw	155	11	aliquots	aliquot	NNS
cord-308835-999kewdw	155	12	of	of	IN
cord-308835-999kewdw	155	13	the	the	DT
cord-308835-999kewdw	155	14	gradients	gradient	NNS
cord-308835-999kewdw	155	15	,	,	,
cord-308835-999kewdw	155	16	treated	treat	VBN
cord-308835-999kewdw	155	17	with	with	IN
cord-308835-999kewdw	155	18	proteinase	proteinase	NN
cord-308835-999kewdw	155	19	K	k	NN
cord-308835-999kewdw	155	20	and	and	CC
cord-308835-999kewdw	155	21	extracted	extract	VBN
cord-308835-999kewdw	155	22	with	with	IN
cord-308835-999kewdw	155	23	phenol	phenol	NN
cord-308835-999kewdw	155	24	chloroform	chloroform	NN
cord-308835-999kewdw	155	25	.	.	.
cord-308835-999kewdw	156	1	The	the	DT
cord-308835-999kewdw	156	2	RNA	RNA	NNP
cord-308835-999kewdw	156	3	was	be	VBD
cord-308835-999kewdw	156	4	precipitated	precipitate	VBN
cord-308835-999kewdw	156	5	with	with	IN
cord-308835-999kewdw	156	6	ethanol	ethanol	NN
cord-308835-999kewdw	156	7	,	,	,
cord-308835-999kewdw	156	8	collected	collect	VBN
cord-308835-999kewdw	156	9	by	by	IN
cord-308835-999kewdw	156	10	centrifugation	centrifugation	NN
cord-308835-999kewdw	156	11	,	,	,
cord-308835-999kewdw	156	12	and	and	CC
cord-308835-999kewdw	156	13	denatured	denature	VBN
cord-308835-999kewdw	156	14	with	with	IN
cord-308835-999kewdw	156	15	glyoxal	glyoxal	NNP
cord-308835-999kewdw	156	16	prior	prior	RB
cord-308835-999kewdw	156	17	to	to	IN
cord-308835-999kewdw	156	18	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	156	19	.	.	.
cord-308835-999kewdw	157	1	Escherichia	escherichia	NN
cord-308835-999kewdw	157	2	coli	coli	VBZ
cord-308835-999kewdw	157	3	rRNA	rrna	NN
cord-308835-999kewdw	157	4	was	be	VBD
cord-308835-999kewdw	157	5	purchased	purchase	VBN
cord-308835-999kewdw	157	6	from	from	IN
cord-308835-999kewdw	157	7	Miles	Miles	NNP
cord-308835-999kewdw	157	8	Biochemicals	Biochemicals	NNPS
cord-308835-999kewdw	157	9	and	and	CC
cord-308835-999kewdw	157	10	denatured	denature	VBD
cord-308835-999kewdw	157	11	1'7CL	1'7cl	CD
cord-308835-999kewdw	157	12	.	.	.
cord-308835-999kewdw	158	1	1	1	CD
cord-308835-999kewdw	158	2	cells	cell	NNS
cord-308835-999kewdw	158	3	were	be	VBD
cord-308835-999kewdw	158	4	infected	infect	VBN
cord-308835-999kewdw	158	5	with	with	IN
cord-308835-999kewdw	158	6	A59V	A59V	NNP
cord-308835-999kewdw	158	7	,	,	,
cord-308835-999kewdw	158	8	JHMV	JHMV	NNP
cord-308835-999kewdw	158	9	,	,	,
cord-308835-999kewdw	158	10	or	or	CC
cord-308835-999kewdw	158	11	mock	mock	RB
cord-308835-999kewdw	158	12	-	-	HYPH
cord-308835-999kewdw	158	13	infected	infected	JJ
cord-308835-999kewdw	158	14	,	,	,
cord-308835-999kewdw	158	15	resuspended	resuspend	VBN
cord-308835-999kewdw	158	16	in	in	IN
cord-308835-999kewdw	158	17	DME2	DME2	NNP
cord-308835-999kewdw	158	18	,	,	,
cord-308835-999kewdw	158	19	seeded	seed	VBN
cord-308835-999kewdw	158	20	in	in	IN
cord-308835-999kewdw	158	21	replicate	replicate	VB
cord-308835-999kewdw	158	22	35-mm	35-mm	CD
cord-308835-999kewdw	158	23	dishes	dish	NNS
cord-308835-999kewdw	158	24	,	,	,
cord-308835-999kewdw	158	25	and	and	CC
cord-308835-999kewdw	158	26	incubated	incubate	VBN
cord-308835-999kewdw	158	27	at	at	IN
cord-308835-999kewdw	158	28	3'7	3'7	CD
cord-308835-999kewdw	158	29	"	"	''
cord-308835-999kewdw	158	30	.	.	.
cord-308835-999kewdw	159	1	At	at	IN
cord-308835-999kewdw	159	2	1	1	CD
cord-308835-999kewdw	159	3	hr	hr	NN
cord-308835-999kewdw	159	4	intervals	interval	NNS
cord-308835-999kewdw	159	5	postinfection	postinfection	NN
cord-308835-999kewdw	159	6	actinomycin	actinomycin	NNS
cord-308835-999kewdw	159	7	D	D	NNP
cord-308835-999kewdw	159	8	was	be	VBD
cord-308835-999kewdw	159	9	added	add	VBN
cord-308835-999kewdw	159	10	to	to	IN
cord-308835-999kewdw	159	11	5	5	CD
cord-308835-999kewdw	159	12	pg	pg	NN
cord-308835-999kewdw	159	13	/	/	SYM
cord-308835-999kewdw	159	14	ml	ml	NN
cord-308835-999kewdw	159	15	to	to	TO
cord-308835-999kewdw	159	16	triplicate	triplicate	VB
cord-308835-999kewdw	159	17	dishes	dish	NNS
cord-308835-999kewdw	159	18	.	.	.
cord-308835-999kewdw	160	1	The	the	DT
cord-308835-999kewdw	160	2	dishes	dish	NNS
cord-308835-999kewdw	160	3	were	be	VBD
cord-308835-999kewdw	160	4	incubated	incubate	VBN
cord-308835-999kewdw	160	5	for	for	IN
cord-308835-999kewdw	160	6	15	15	CD
cord-308835-999kewdw	160	7	min	min	NN
cord-308835-999kewdw	160	8	and	and	CC
cord-308835-999kewdw	160	9	then	then	RB
cord-308835-999kewdw	160	10	labeled	label	VBD
cord-308835-999kewdw	160	11	for	for	IN
cord-308835-999kewdw	160	12	1	1	CD
cord-308835-999kewdw	160	13	hr	hr	NN
cord-308835-999kewdw	160	14	with	with	IN
cord-308835-999kewdw	160	15	5	5	CD
cord-308835-999kewdw	160	16	&	&	CC
cord-308835-999kewdw	160	17	i	i	NNP
cord-308835-999kewdw	160	18	/	/	SYM
cord-308835-999kewdw	160	19	ml	ml	NN
cord-308835-999kewdw	160	20	of	of	IN
cord-308835-999kewdw	160	21	[	[	-LRB-
cord-308835-999kewdw	160	22	'	'	``
cord-308835-999kewdw	160	23	Hjuridine	Hjuridine	NNP
cord-308835-999kewdw	160	24	.	.	.
cord-308835-999kewdw	161	1	At	at	IN
cord-308835-999kewdw	161	2	the	the	DT
cord-308835-999kewdw	161	3	end	end	NN
cord-308835-999kewdw	161	4	of	of	IN
cord-308835-999kewdw	161	5	the	the	DT
cord-308835-999kewdw	161	6	labeling	labeling	NN
cord-308835-999kewdw	161	7	period	period	NN
cord-308835-999kewdw	161	8	the	the	DT
cord-308835-999kewdw	161	9	dishes	dish	NNS
cord-308835-999kewdw	161	10	were	be	VBD
cord-308835-999kewdw	161	11	removed	remove	VBN
cord-308835-999kewdw	161	12	from	from	IN
cord-308835-999kewdw	161	13	the	the	DT
cord-308835-999kewdw	161	14	incubator	incubator	NN
cord-308835-999kewdw	161	15	,	,	,
cord-308835-999kewdw	161	16	washed	wash	VBN
cord-308835-999kewdw	161	17	twice	twice	RB
cord-308835-999kewdw	161	18	with	with	IN
cord-308835-999kewdw	161	19	cold	cold	JJ
cord-308835-999kewdw	161	20	phosphate	phosphate	NN
cord-308835-999kewdw	161	21	-	-	HYPH
cord-308835-999kewdw	161	22	buffered	buffer	VBN
cord-308835-999kewdw	161	23	saline	saline	NN
cord-308835-999kewdw	161	24	(	(	-LRB-
cord-308835-999kewdw	161	25	PBS	PBS	NNP
cord-308835-999kewdw	161	26	)	)	-RRB-
cord-308835-999kewdw	161	27	,	,	,
cord-308835-999kewdw	161	28	scraped	scrape	VBD
cord-308835-999kewdw	161	29	from	from	IN
cord-308835-999kewdw	161	30	the	the	DT
cord-308835-999kewdw	161	31	dishes	dish	NNS
cord-308835-999kewdw	161	32	into	into	IN
cord-308835-999kewdw	161	33	cold	cold	JJ
cord-308835-999kewdw	161	34	PBS	PBS	NNP
cord-308835-999kewdw	161	35	,	,	,
cord-308835-999kewdw	161	36	and	and	CC
cord-308835-999kewdw	161	37	frozen	freeze	VBN
cord-308835-999kewdw	161	38	at	at	IN
cord-308835-999kewdw	161	39	-20	-20	NN
cord-308835-999kewdw	161	40	"	"	''
cord-308835-999kewdw	161	41	.	.	.
cord-308835-999kewdw	162	1	The	the	DT
cord-308835-999kewdw	162	2	samples	sample	NNS
cord-308835-999kewdw	162	3	were	be	VBD
cord-308835-999kewdw	162	4	thawed	thaw	VBN
cord-308835-999kewdw	162	5	24	24	CD
cord-308835-999kewdw	162	6	hr	hr	NN
cord-308835-999kewdw	162	7	later	later	RB
cord-308835-999kewdw	162	8	and	and	CC
cord-308835-999kewdw	162	9	the	the	DT
cord-308835-999kewdw	162	10	amount	amount	NN
cord-308835-999kewdw	162	11	of	of	IN
cord-308835-999kewdw	162	12	trichloroacetic	trichloroacetic	JJ
cord-308835-999kewdw	162	13	acid	acid	NN
cord-308835-999kewdw	162	14	precipitable	precipitable	JJ
cord-308835-999kewdw	162	15	radioactivity	radioactivity	NN
cord-308835-999kewdw	162	16	determined	determine	VBN
cord-308835-999kewdw	162	17	.	.	.
cord-308835-999kewdw	163	1	The	the	DT
cord-308835-999kewdw	163	2	results	result	NNS
cord-308835-999kewdw	163	3	are	be	VBP
cord-308835-999kewdw	163	4	expressed	express	VBN
cord-308835-999kewdw	163	5	as	as	IN
cord-308835-999kewdw	163	6	the	the	DT
cord-308835-999kewdw	163	7	mean	mean	NN
cord-308835-999kewdw	163	8	of	of	IN
cord-308835-999kewdw	163	9	triplicate	triplicate	NN
cord-308835-999kewdw	163	10	samples	sample	NNS
cord-308835-999kewdw	163	11	.	.	.
cord-308835-999kewdw	164	1	The	the	DT
cord-308835-999kewdw	164	2	amount	amount	NN
cord-308835-999kewdw	164	3	of	of	IN
cord-308835-999kewdw	164	4	radioactivity	radioactivity	NN
cord-308835-999kewdw	164	5	incorporated	incorporate	VBN
cord-308835-999kewdw	164	6	into	into	IN
cord-308835-999kewdw	164	7	mock	mock	JJ
cord-308835-999kewdw	164	8	infected	infected	JJ
cord-308835-999kewdw	164	9	dishes	dish	NNS
cord-308835-999kewdw	164	10	(	(	-LRB-
cord-308835-999kewdw	164	11	about	about	RB
cord-308835-999kewdw	164	12	2000	2000	CD
cord-308835-999kewdw	164	13	cpm	cpm	NN
cord-308835-999kewdw	164	14	)	)	-RRB-
cord-308835-999kewdw	164	15	has	have	VBZ
cord-308835-999kewdw	164	16	been	be	VBN
cord-308835-999kewdw	164	17	substracted	substracte	VBN
cord-308835-999kewdw	164	18	from	from	IN
cord-308835-999kewdw	164	19	the	the	DT
cord-308835-999kewdw	164	20	values	value	NNS
cord-308835-999kewdw	164	21	shown	show	VBN
cord-308835-999kewdw	164	22	.	.	.
cord-308835-999kewdw	165	1	with	with	IN
cord-308835-999kewdw	165	2	glyoxal	glyoxal	NNP
cord-308835-999kewdw	165	3	prior	prior	RB
cord-308835-999kewdw	165	4	to	to	IN
cord-308835-999kewdw	165	5	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	165	6	.	.	.
cord-308835-999kewdw	166	1	The	the	DT
cord-308835-999kewdw	166	2	16	16	CD
cord-308835-999kewdw	166	3	and	and	CC
cord-308835-999kewdw	166	4	23	23	CD
cord-308835-999kewdw	166	5	S	S	NNS
cord-308835-999kewdw	166	6	rRNAs	rrna	NNS
cord-308835-999kewdw	166	7	were	be	VBD
cord-308835-999kewdw	166	8	located	locate	VBN
cord-308835-999kewdw	166	9	following	follow	VBG
cord-308835-999kewdw	166	10	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	166	11	by	by	IN
cord-308835-999kewdw	166	12	staining	stain	VBG
cord-308835-999kewdw	166	13	with	with	IN
cord-308835-999kewdw	166	14	acridine	acridine	JJ
cord-308835-999kewdw	166	15	orange	orange	NN
cord-308835-999kewdw	166	16	and	and	CC
cord-308835-999kewdw	166	17	their	-PRON-	PRP$
cord-308835-999kewdw	166	18	locations	location	NNS
cord-308835-999kewdw	166	19	marked	mark	VBN
cord-308835-999kewdw	166	20	with	with	IN
cord-308835-999kewdw	166	21	wire	wire	NN
cord-308835-999kewdw	166	22	clips	clip	NNS
cord-308835-999kewdw	166	23	inserted	insert	VBN
cord-308835-999kewdw	166	24	in	in	IN
cord-308835-999kewdw	166	25	the	the	DT
cord-308835-999kewdw	166	26	gel	gel	NN
cord-308835-999kewdw	166	27	.	.	.
cord-308835-999kewdw	167	1	Uridine	Uridine	NNP
cord-308835-999kewdw	167	2	Incorporation	Incorporation	NNP
cord-308835-999kewdw	167	3	Robb	Robb	NNP
cord-308835-999kewdw	167	4	and	and	CC
cord-308835-999kewdw	167	5	Bond	Bond	NNP
cord-308835-999kewdw	167	6	(	(	-LRB-
cord-308835-999kewdw	167	7	1979b	1979b	CD
cord-308835-999kewdw	167	8	)	)	-RRB-
cord-308835-999kewdw	167	9	have	have	VBP
cord-308835-999kewdw	167	10	shown	show	VBN
cord-308835-999kewdw	167	11	that	that	IN
cord-308835-999kewdw	167	12	MHV	mhv	NN
cord-308835-999kewdw	167	13	-	-	HYPH
cord-308835-999kewdw	167	14	specific	specific	JJ
cord-308835-999kewdw	167	15	RNA	RNA	NNP
cord-308835-999kewdw	167	16	synthesis	synthesis	NN
cord-308835-999kewdw	167	17	is	be	VBZ
cord-308835-999kewdw	167	18	resistant	resistant	JJ
cord-308835-999kewdw	167	19	to	to	IN
cord-308835-999kewdw	167	20	actinomycin	actinomycin	NNS
cord-308835-999kewdw	167	21	D.	D.	NNP
cord-308835-999kewdw	168	1	We	-PRON-	PRP
cord-308835-999kewdw	168	2	have	have	VBP
cord-308835-999kewdw	168	3	taken	take	VBN
cord-308835-999kewdw	168	4	advantage	advantage	NN
cord-308835-999kewdw	168	5	of	of	IN
cord-308835-999kewdw	168	6	this	this	DT
cord-308835-999kewdw	168	7	to	to	TO
cord-308835-999kewdw	168	8	determine	determine	VB
cord-308835-999kewdw	168	9	the	the	DT
cord-308835-999kewdw	168	10	kinetics	kinetic	NNS
cord-308835-999kewdw	168	11	of	of	IN
cord-308835-999kewdw	168	12	MHV	MHV	NNP
cord-308835-999kewdw	168	13	-	-	HYPH
cord-308835-999kewdw	168	14	specific	specific	JJ
cord-308835-999kewdw	168	15	RNA	RNA	NNP
cord-308835-999kewdw	168	16	synthesis	synthesis	NN
cord-308835-999kewdw	168	17	by	by	IN
cord-308835-999kewdw	168	18	assaying	assay	VBG
cord-308835-999kewdw	168	19	the	the	DT
cord-308835-999kewdw	168	20	incorporation	incorporation	NN
cord-308835-999kewdw	168	21	of	of	IN
cord-308835-999kewdw	168	22	rH]uridine	rH]uridine	NNP
cord-308835-999kewdw	168	23	(	(	-LRB-
cord-308835-999kewdw	168	24	in	in	IN
cord-308835-999kewdw	168	25	the	the	DT
cord-308835-999kewdw	168	26	presence	presence	NN
cord-308835-999kewdw	168	27	of	of	IN
cord-308835-999kewdw	168	28	actinomycin	actinomycin	NNS
cord-308835-999kewdw	168	29	D	D	NNP
cord-308835-999kewdw	168	30	)	)	-RRB-
cord-308835-999kewdw	168	31	into	into	IN
cord-308835-999kewdw	168	32	acid	acid	NN
cord-308835-999kewdw	168	33	-	-	HYPH
cord-308835-999kewdw	168	34	precipitable	precipitable	JJ
cord-308835-999kewdw	168	35	material	material	NN
cord-308835-999kewdw	168	36	.	.	.
cord-308835-999kewdw	169	1	17CL	17cl	CD
cord-308835-999kewdw	169	2	.	.	.
cord-308835-999kewdw	170	1	1	1	CD
cord-308835-999kewdw	170	2	cells	cell	NNS
cord-308835-999kewdw	170	3	were	be	VBD
cord-308835-999kewdw	170	4	in	in	RB
cord-308835-999kewdw	170	5	-	-	HYPH
cord-308835-999kewdw	170	6	fected	fecte	VBN
cord-308835-999kewdw	170	7	with	with	IN
cord-308835-999kewdw	170	8	JHMV	JHMV	NNP
cord-308835-999kewdw	170	9	,	,	,
cord-308835-999kewdw	170	10	A59V	A59V	NNP
cord-308835-999kewdw	170	11	,	,	,
cord-308835-999kewdw	170	12	or	or	CC
cord-308835-999kewdw	170	13	mock	mock	VB
cord-308835-999kewdw	170	14	infected	infect	VBN
cord-308835-999kewdw	170	15	and	and	CC
cord-308835-999kewdw	170	16	at	at	IN
cord-308835-999kewdw	170	17	hourly	hourly	JJ
cord-308835-999kewdw	170	18	intervals	interval	NNS
cord-308835-999kewdw	170	19	replicate	replicate	VBP
cord-308835-999kewdw	170	20	dishes	dish	NNS
cord-308835-999kewdw	170	21	were	be	VBD
cord-308835-999kewdw	170	22	incubated	incubate	VBN
cord-308835-999kewdw	170	23	for	for	IN
cord-308835-999kewdw	170	24	15	15	CD
cord-308835-999kewdw	170	25	min	min	NN
cord-308835-999kewdw	170	26	with	with	IN
cord-308835-999kewdw	170	27	actinomycin	actinomycin	NNS
cord-308835-999kewdw	170	28	D	D	NNP
cord-308835-999kewdw	170	29	and	and	CC
cord-308835-999kewdw	170	30	then	then	RB
cord-308835-999kewdw	170	31	labeled	label	VBD
cord-308835-999kewdw	170	32	for	for	IN
cord-308835-999kewdw	170	33	1	1	CD
cord-308835-999kewdw	170	34	hr	hr	NN
cord-308835-999kewdw	170	35	in	in	IN
cord-308835-999kewdw	170	36	the	the	DT
cord-308835-999kewdw	170	37	presence	presence	NN
cord-308835-999kewdw	170	38	of	of	IN
cord-308835-999kewdw	170	39	the	the	DT
cord-308835-999kewdw	170	40	drug	drug	NN
cord-308835-999kewdw	170	41	.	.	.
cord-308835-999kewdw	171	1	The	the	DT
cord-308835-999kewdw	171	2	results	result	NNS
cord-308835-999kewdw	171	3	of	of	IN
cord-308835-999kewdw	171	4	this	this	DT
cord-308835-999kewdw	171	5	experiment	experiment	NN
cord-308835-999kewdw	171	6	are	be	VBP
cord-308835-999kewdw	171	7	shown	show	VBN
cord-308835-999kewdw	171	8	in	in	IN
cord-308835-999kewdw	171	9	Fig	Fig	NNP
cord-308835-999kewdw	171	10	.	.	.
cord-308835-999kewdw	172	1	1	1	LS
cord-308835-999kewdw	172	2	.	.	.
cord-308835-999kewdw	173	1	After	after	IN
cord-308835-999kewdw	173	2	an	an	DT
cord-308835-999kewdw	173	3	initial	initial	JJ
cord-308835-999kewdw	173	4	lag	lag	NN
cord-308835-999kewdw	173	5	phase	phase	NN
cord-308835-999kewdw	173	6	of	of	IN
cord-308835-999kewdw	173	7	3	3	CD
cord-308835-999kewdw	173	8	hr	hr	NN
cord-308835-999kewdw	173	9	in	in	IN
cord-308835-999kewdw	173	10	A59V	a59v	NN
cord-308835-999kewdw	173	11	-	-	HYPH
cord-308835-999kewdw	173	12	infected	infect	VBN
cord-308835-999kewdw	173	13	cells	cell	NNS
cord-308835-999kewdw	173	14	and	and	CC
cord-308835-999kewdw	173	15	5	5	CD
cord-308835-999kewdw	173	16	hr	hr	NN
cord-308835-999kewdw	173	17	in	in	IN
cord-308835-999kewdw	173	18	JHMV	JHMV	NNP
cord-308835-999kewdw	173	19	-	-	HYPH
cord-308835-999kewdw	173	20	infected	infect	VBN
cord-308835-999kewdw	173	21	cells	cell	NNS
cord-308835-999kewdw	173	22	,	,	,
cord-308835-999kewdw	173	23	actinomycin	actinomycin	NNS
cord-308835-999kewdw	173	24	D	d	NN
cord-308835-999kewdw	173	25	-	-	HYPH
cord-308835-999kewdw	173	26	resistant	resistant	JJ
cord-308835-999kewdw	173	27	uridine	uridine	NN
cord-308835-999kewdw	173	28	incorporation	incorporation	NN
cord-308835-999kewdw	173	29	increases	increase	NNS
cord-308835-999kewdw	173	30	exponentially	exponentially	RB
cord-308835-999kewdw	173	31	until	until	IN
cord-308835-999kewdw	173	32	7	7	CD
cord-308835-999kewdw	173	33	hpi	hpi	NNP
cord-308835-999kewdw	173	34	,	,	,
cord-308835-999kewdw	173	35	when	when	WRB
cord-308835-999kewdw	173	36	it	-PRON-	PRP
cord-308835-999kewdw	173	37	plateaus	plateaus	VBP
cord-308835-999kewdw	173	38	.	.	.
cord-308835-999kewdw	174	1	These	these	DT
cord-308835-999kewdw	174	2	results	result	NNS
cord-308835-999kewdw	174	3	are	be	VBP
cord-308835-999kewdw	174	4	consistent	consistent	JJ
cord-308835-999kewdw	174	5	with	with	IN
cord-308835-999kewdw	174	6	the	the	DT
cord-308835-999kewdw	174	7	one	one	CD
cord-308835-999kewdw	174	8	step	step	NN
cord-308835-999kewdw	174	9	growth	growth	NN
cord-308835-999kewdw	174	10	curves	curve	NNS
cord-308835-999kewdw	174	11	of	of	IN
cord-308835-999kewdw	174	12	these	these	DT
cord-308835-999kewdw	174	13	two	two	CD
cord-308835-999kewdw	174	14	viruses	virus	NNS
cord-308835-999kewdw	174	15	(	(	-LRB-
cord-308835-999kewdw	174	16	Bond	Bond	NNP
cord-308835-999kewdw	174	17	et	et	NNP
cord-308835-999kewdw	174	18	al	al	NNP
cord-308835-999kewdw	174	19	.	.	NNP
cord-308835-999kewdw	174	20	,	,	,
cord-308835-999kewdw	174	21	1979	1979	CD
cord-308835-999kewdw	174	22	)	)	-RRB-
cord-308835-999kewdw	174	23	.	.	.
cord-308835-999kewdw	175	1	In	in	IN
cord-308835-999kewdw	175	2	view	view	NN
cord-308835-999kewdw	175	3	of	of	IN
cord-308835-999kewdw	175	4	the	the	DT
cord-308835-999kewdw	175	5	above	above	JJ
cord-308835-999kewdw	175	6	results	result	NNS
cord-308835-999kewdw	175	7	,	,	,
cord-308835-999kewdw	175	8	we	-PRON-	PRP
cord-308835-999kewdw	175	9	routinely	routinely	RB
cord-308835-999kewdw	175	10	labeled	label	VBD
cord-308835-999kewdw	175	11	cells	cell	NNS
cord-308835-999kewdw	175	12	from	from	IN
cord-308835-999kewdw	175	13	4	4	CD
cord-308835-999kewdw	175	14	to	to	TO
cord-308835-999kewdw	175	15	8	8	CD
cord-308835-999kewdw	175	16	hpi	hpi	NN
cord-308835-999kewdw	175	17	in	in	IN
cord-308835-999kewdw	175	18	subsequent	subsequent	JJ
cord-308835-999kewdw	175	19	experiments	experiment	NNS
cord-308835-999kewdw	175	20	.	.	.
cord-308835-999kewdw	176	1	Identification	identification	NN
cord-308835-999kewdw	176	2	of	of	IN
cord-308835-999kewdw	176	3	MHV	MHV	NNP
cord-308835-999kewdw	176	4	-	-	HYPH
cord-308835-999kewdw	176	5	Specific	Specific	NNP
cord-308835-999kewdw	176	6	RNA	RNA	NNP
cord-308835-999kewdw	176	7	Species	species	NN
cord-308835-999kewdw	176	8	A59V	A59V	NNP
cord-308835-999kewdw	176	9	,	,	,
cord-308835-999kewdw	176	10	JHMV	JHMV	NNP
cord-308835-999kewdw	176	11	,	,	,
cord-308835-999kewdw	176	12	and	and	CC
cord-308835-999kewdw	176	13	mock	mock	JJ
cord-308835-999kewdw	176	14	-	-	HYPH
cord-308835-999kewdw	176	15	infected	infect	VBN
cord-308835-999kewdw	176	16	cells	cell	NNS
cord-308835-999kewdw	176	17	were	be	VBD
cord-308835-999kewdw	176	18	labeled	label	VBN
cord-308835-999kewdw	176	19	with	with	IN
cord-308835-999kewdw	176	20	[	[	-LRB-
cord-308835-999kewdw	176	21	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	176	22	from	from	IN
cord-308835-999kewdw	176	23	4	4	CD
cord-308835-999kewdw	176	24	to	to	TO
cord-308835-999kewdw	176	25	8	8	CD
cord-308835-999kewdw	176	26	hpi	hpi	NNS
cord-308835-999kewdw	176	27	in	in	IN
cord-308835-999kewdw	176	28	the	the	DT
cord-308835-999kewdw	176	29	presence	presence	NN
cord-308835-999kewdw	176	30	of	of	IN
cord-308835-999kewdw	176	31	actinomycin	actinomycin	NNS
cord-308835-999kewdw	176	32	D.	D.	NNP
cord-308835-999kewdw	177	1	The	the	DT
cord-308835-999kewdw	177	2	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	177	3	RNA	RNA	NNP
cord-308835-999kewdw	177	4	was	be	VBD
cord-308835-999kewdw	177	5	extracted	extract	VBN
cord-308835-999kewdw	177	6	,	,	,
cord-308835-999kewdw	177	7	denatured	denature	VBN
cord-308835-999kewdw	177	8	with	with	IN
cord-308835-999kewdw	177	9	glyoxal	glyoxal	NNP
cord-308835-999kewdw	177	10	,	,	,
cord-308835-999kewdw	177	11	and	and	CC
cord-308835-999kewdw	177	12	analyzed	analyze	VBN
cord-308835-999kewdw	177	13	by	by	IN
cord-308835-999kewdw	177	14	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	177	15	on	on	IN
cord-308835-999kewdw	177	16	an	an	DT
cord-308835-999kewdw	177	17	agarose	agarose	JJ
cord-308835-999kewdw	177	18	gel	gel	NN
cord-308835-999kewdw	177	19	(	(	-LRB-
cord-308835-999kewdw	177	20	Fig	fig	NN
cord-308835-999kewdw	177	21	.	.	NNP
cord-308835-999kewdw	177	22	2	2	CD
cord-308835-999kewdw	177	23	)	)	-RRB-
cord-308835-999kewdw	177	24	.	.	.
cord-308835-999kewdw	178	1	Seven	seven	CD
cord-308835-999kewdw	178	2	RNA	RNA	NNP
cord-308835-999kewdw	178	3	species	specie	NNS
cord-308835-999kewdw	178	4	,	,	,
cord-308835-999kewdw	178	5	designated	designate	VBN
cord-308835-999kewdw	178	6	l-7	l-7	NN
cord-308835-999kewdw	178	7	in	in	IN
cord-308835-999kewdw	178	8	decreasing	decrease	VBG
cord-308835-999kewdw	178	9	order	order	NN
cord-308835-999kewdw	178	10	of	of	IN
cord-308835-999kewdw	178	11	size	size	NN
cord-308835-999kewdw	178	12	,	,	,
cord-308835-999kewdw	178	13	were	be	VBD
cord-308835-999kewdw	178	14	reproducibly	reproducibly	RB
cord-308835-999kewdw	178	15	present	present	JJ
cord-308835-999kewdw	178	16	in	in	IN
cord-308835-999kewdw	178	17	JHMV	JHMV	NNP
cord-308835-999kewdw	178	18	-	-	HYPH
cord-308835-999kewdw	178	19	and	and	CC
cord-308835-999kewdw	178	20	A59Vinfected	a59vinfected	JJ
cord-308835-999kewdw	178	21	cells	cell	NNS
cord-308835-999kewdw	178	22	.	.	.
cord-308835-999kewdw	179	1	JHMV	JHMV	NNP
cord-308835-999kewdw	179	2	-	-	HYPH
cord-308835-999kewdw	179	3	infected	infect	VBN
cord-308835-999kewdw	179	4	cells	cell	NNS
cord-308835-999kewdw	179	5	synthesize	synthesize	VBP
cord-308835-999kewdw	179	6	less	less	JJR
cord-308835-999kewdw	179	7	RNA5	RNA5	NNP
cord-308835-999kewdw	179	8	than	than	IN
cord-308835-999kewdw	179	9	A59V	A59V	NNP
cord-308835-999kewdw	179	10	-	-	HYPH
cord-308835-999kewdw	179	11	infected	infect	VBN
cord-308835-999kewdw	179	12	cells	cell	NNS
cord-308835-999kewdw	179	13	(	(	-LRB-
cord-308835-999kewdw	179	14	Table	table	NN
cord-308835-999kewdw	179	15	1	1	CD
cord-308835-999kewdw	179	16	)	)	-RRB-
cord-308835-999kewdw	179	17	.	.	.
cord-308835-999kewdw	180	1	Although	although	IN
cord-308835-999kewdw	180	2	the	the	DT
cord-308835-999kewdw	180	3	band	band	NN
cord-308835-999kewdw	180	4	representing	represent	VBG
cord-308835-999kewdw	180	5	JHMV	JHMV	NNP
cord-308835-999kewdw	180	6	-	-	HYPH
cord-308835-999kewdw	180	7	specific	specific	JJ
cord-308835-999kewdw	180	8	RNA5	RNA5	NNP
cord-308835-999kewdw	180	9	is	be	VBZ
cord-308835-999kewdw	180	10	barely	barely	RB
cord-308835-999kewdw	180	11	visible	visible	JJ
cord-308835-999kewdw	180	12	in	in	IN
cord-308835-999kewdw	180	13	Fig	Fig	NNP
cord-308835-999kewdw	180	14	.	.	.
cord-308835-999kewdw	181	1	2	2	LS
cord-308835-999kewdw	181	2	(	(	-LRB-
cord-308835-999kewdw	181	3	lane	lane	NNP
cord-308835-999kewdw	181	4	J	J	NNP
cord-308835-999kewdw	181	5	)	)	-RRB-
cord-308835-999kewdw	181	6	,	,	,
cord-308835-999kewdw	181	7	this	this	DT
cord-308835-999kewdw	181	8	RNA	RNA	NNP
cord-308835-999kewdw	181	9	species	specie	NNS
cord-308835-999kewdw	181	10	could	could	MD
cord-308835-999kewdw	181	11	be	be	VB
cord-308835-999kewdw	181	12	seen	see	VBN
cord-308835-999kewdw	181	13	with	with	IN
cord-308835-999kewdw	181	14	a	a	DT
cord-308835-999kewdw	181	15	longer	long	JJR
cord-308835-999kewdw	181	16	exposure	exposure	NN
cord-308835-999kewdw	181	17	of	of	IN
cord-308835-999kewdw	181	18	the	the	DT
cord-308835-999kewdw	181	19	autoradiograph	autoradiograph	NNP
cord-308835-999kewdw	181	20	,	,	,
cord-308835-999kewdw	181	21	was	be	VBD
cord-308835-999kewdw	181	22	reproducibly	reproducibly	RB
cord-308835-999kewdw	181	23	present	present	JJ
cord-308835-999kewdw	181	24	in	in	IN
cord-308835-999kewdw	181	25	infected	infected	JJ
cord-308835-999kewdw	181	26	cell	cell	NN
cord-308835-999kewdw	181	27	extracts	extract	NNS
cord-308835-999kewdw	181	28	,	,	,
cord-308835-999kewdw	181	29	and	and	CC
cord-308835-999kewdw	181	30	can	can	MD
cord-308835-999kewdw	181	31	be	be	VB
cord-308835-999kewdw	181	32	better	well	RBR
cord-308835-999kewdw	181	33	seen	see	VBN
cord-308835-999kewdw	181	34	in	in	IN
cord-308835-999kewdw	181	35	Fig	Fig	NNP
cord-308835-999kewdw	181	36	.	.	.
cord-308835-999kewdw	182	1	6	6	LS
cord-308835-999kewdw	182	2	.	.	.
cord-308835-999kewdw	183	1	In	in	IN
cord-308835-999kewdw	183	2	addition	addition	NN
cord-308835-999kewdw	183	3	,	,	,
cord-308835-999kewdw	183	4	three	three	CD
cord-308835-999kewdw	183	5	minor	minor	JJ
cord-308835-999kewdw	183	6	RNA	RNA	NNP
cord-308835-999kewdw	183	7	species	specie	NNS
cord-308835-999kewdw	183	8	were	be	VBD
cord-308835-999kewdw	183	9	often	often	RB
cord-308835-999kewdw	183	10	observed	observe	VBN
cord-308835-999kewdw	183	11	,	,	,
cord-308835-999kewdw	183	12	although	although	IN
cord-308835-999kewdw	183	13	not	not	RB
cord-308835-999kewdw	183	14	in	in	IN
cord-308835-999kewdw	183	15	every	every	DT
cord-308835-999kewdw	183	16	experiment	experiment	NN
cord-308835-999kewdw	183	17	.	.	.
cord-308835-999kewdw	184	1	The	the	DT
cord-308835-999kewdw	184	2	largest	large	JJS
cord-308835-999kewdw	184	3	of	of	IN
cord-308835-999kewdw	184	4	these	these	DT
cord-308835-999kewdw	184	5	RNAs	rna	NNS
cord-308835-999kewdw	184	6	migrated	migrate	VBD
cord-308835-999kewdw	184	7	between	between	IN
cord-308835-999kewdw	184	8	RNAs	rna	NNS
cord-308835-999kewdw	184	9	3	3	CD
cord-308835-999kewdw	184	10	and	and	CC
cord-308835-999kewdw	184	11	4	4	CD
cord-308835-999kewdw	184	12	just	just	RB
cord-308835-999kewdw	184	13	faster	fast	JJR
cord-308835-999kewdw	184	14	than	than	IN
cord-308835-999kewdw	184	15	28	28	CD
cord-308835-999kewdw	185	1	S	S	NNP
cord-308835-999kewdw	185	2	rRNA	rrna	NN
cord-308835-999kewdw	185	3	.	.	.
cord-308835-999kewdw	186	1	Two	two	CD
cord-308835-999kewdw	186	2	minor	minor	JJ
cord-308835-999kewdw	186	3	RNAs	rna	NNS
cord-308835-999kewdw	186	4	migrating	migrate	VBG
cord-308835-999kewdw	186	5	faster	fast	RBR
cord-308835-999kewdw	186	6	than	than	IN
cord-308835-999kewdw	186	7	RNA7	RNA7	NNP
cord-308835-999kewdw	186	8	were	be	VBD
cord-308835-999kewdw	186	9	also	also	RB
cord-308835-999kewdw	186	10	sometimes	sometimes	RB
cord-308835-999kewdw	186	11	observed	observe	VBN
cord-308835-999kewdw	186	12	.	.	.
cord-308835-999kewdw	187	1	These	these	DT
cord-308835-999kewdw	187	2	RNAs	rna	NNS
cord-308835-999kewdw	187	3	were	be	VBD
cord-308835-999kewdw	187	4	not	not	RB
cord-308835-999kewdw	187	5	studied	study	VBN
cord-308835-999kewdw	187	6	extensively	extensively	RB
cord-308835-999kewdw	187	7	due	due	JJ
cord-308835-999kewdw	187	8	to	to	IN
cord-308835-999kewdw	187	9	difficulty	difficulty	NN
cord-308835-999kewdw	187	10	in	in	IN
cord-308835-999kewdw	187	11	preparing	prepare	VBG
cord-308835-999kewdw	187	12	sufficient	sufficient	JJ
cord-308835-999kewdw	187	13	material	material	NN
cord-308835-999kewdw	187	14	and	and	CC
cord-308835-999kewdw	187	15	their	-PRON-	PRP$
cord-308835-999kewdw	187	16	inconsistent	inconsistent	JJ
cord-308835-999kewdw	187	17	appearance	appearance	NN
cord-308835-999kewdw	187	18	.	.	.
cord-308835-999kewdw	188	1	No	no	DT
cord-308835-999kewdw	188	2	discrete	discrete	JJ
cord-308835-999kewdw	188	3	bands	band	NNS
cord-308835-999kewdw	188	4	were	be	VBD
cord-308835-999kewdw	188	5	identified	identify	VBN
cord-308835-999kewdw	188	6	in	in	IN
cord-308835-999kewdw	188	7	mock	mock	JJ
cord-308835-999kewdw	188	8	-	-	HYPH
cord-308835-999kewdw	188	9	infected	infect	VBN
cord-308835-999kewdw	188	10	extracts	extract	NNS
cord-308835-999kewdw	188	11	.	.	.
cord-308835-999kewdw	189	1	The	the	DT
cord-308835-999kewdw	189	2	largest	large	JJS
cord-308835-999kewdw	189	3	MHV	mhv	NN
cord-308835-999kewdw	189	4	-	-	HYPH
cord-308835-999kewdw	189	5	specific	specific	JJ
cord-308835-999kewdw	189	6	RNA	RNA	NNP
cord-308835-999kewdw	189	7	,	,	,
cord-308835-999kewdw	189	8	RNA	RNA	NNP
cord-308835-999kewdw	189	9	1	1	CD
cord-308835-999kewdw	189	10	,	,	,
cord-308835-999kewdw	189	11	coelectrophoreses	coelectrophoreses	NNPS
cord-308835-999kewdw	189	12	with	with	IN
cord-308835-999kewdw	189	13	virion	virion	NN
cord-308835-999kewdw	189	14	RNA	RNA	NNP
cord-308835-999kewdw	189	15	and	and	CC
cord-308835-999kewdw	189	16	represents	represent	VBZ
cord-308835-999kewdw	189	17	the	the	DT
cord-308835-999kewdw	189	18	intracellular	intracellular	JJ
cord-308835-999kewdw	189	19	form	form	NN
cord-308835-999kewdw	189	20	of	of	IN
cord-308835-999kewdw	189	21	the	the	DT
cord-308835-999kewdw	189	22	genome	genome	NN
cord-308835-999kewdw	189	23	.	.	.
cord-308835-999kewdw	190	1	In	in	IN
cord-308835-999kewdw	190	2	contrast	contrast	NN
cord-308835-999kewdw	190	3	to	to	IN
cord-308835-999kewdw	190	4	the	the	DT
cord-308835-999kewdw	190	5	size	size	NN
cord-308835-999kewdw	190	6	differences	difference	NNS
cord-308835-999kewdw	190	7	we	-PRON-	PRP
cord-308835-999kewdw	190	8	have	have	VBP
cord-308835-999kewdw	190	9	observed	observe	VBN
cord-308835-999kewdw	190	10	between	between	IN
cord-308835-999kewdw	190	11	A59V-	A59V-	NNP
cord-308835-999kewdw	190	12	.	.	.
cord-308835-999kewdw	191	1	and	and	CC
cord-308835-999kewdw	191	2	JHMV	JHMV	NNP
cord-308835-999kewdw	191	3	-	-	HYPH
cord-308835-999kewdw	191	4	specific	specific	JJ
cord-308835-999kewdw	191	5	proteins	protein	NNS
cord-308835-999kewdw	192	1	(	(	-LRB-
cord-308835-999kewdw	192	2	Bond	Bond	NNP
cord-308835-999kewdw	192	3	et	et	NNP
cord-308835-999kewdw	192	4	al	al	NNP
cord-308835-999kewdw	192	5	.	.	NNP
cord-308835-999kewdw	192	6	,	,	,
cord-308835-999kewdw	192	7	1979	1979	CD
cord-308835-999kewdw	192	8	)	)	-RRB-
cord-308835-999kewdw	192	9	,	,	,
cord-308835-999kewdw	192	10	A59V	A59V	NNP
cord-308835-999kewdw	192	11	-	-	:
cord-308835-999kewdw	192	12	and	and	CC
cord-308835-999kewdw	192	13	JHMV	JHMV	NNP
cord-308835-999kewdw	192	14	-	-	HYPH
cord-308835-999kewdw	192	15	specific	specific	JJ
cord-308835-999kewdw	193	1	RNAs	RNAs	NNP
cord-308835-999kewdw	193	2	comigrate	comigrate	VBP
cord-308835-999kewdw	193	3	in	in	IN
cord-308835-999kewdw	193	4	agarose	agarose	JJ
cord-308835-999kewdw	193	5	gels	gel	NNS
cord-308835-999kewdw	193	6	.	.	.
cord-308835-999kewdw	194	1	The	the	DT
cord-308835-999kewdw	194	2	amount	amount	NN
cord-308835-999kewdw	194	3	of	of	IN
cord-308835-999kewdw	194	4	virus	virus	NN
cord-308835-999kewdw	194	5	-	-	HYPH
cord-308835-999kewdw	194	6	specific	specific	JJ
cord-308835-999kewdw	194	7	RNA	RNA	NNP
cord-308835-999kewdw	194	8	synthesized	synthesize	VBN
cord-308835-999kewdw	194	9	in	in	IN
cord-308835-999kewdw	194	10	A59V	a59v	NN
cord-308835-999kewdw	194	11	-	-	HYPH
cord-308835-999kewdw	194	12	infected	infect	VBN
cord-308835-999kewdw	194	13	cells	cell	NNS
cord-308835-999kewdw	194	14	is	be	VBZ
cord-308835-999kewdw	194	15	,	,	,
cord-308835-999kewdw	194	16	however	however	RB
cord-308835-999kewdw	194	17	,	,	,
cord-308835-999kewdw	194	18	about	about	RB
cord-308835-999kewdw	194	19	30	30	CD
cord-308835-999kewdw	194	20	%	%	NN
cord-308835-999kewdw	194	21	greater	great	JJR
cord-308835-999kewdw	194	22	than	than	IN
cord-308835-999kewdw	194	23	that	that	DT
cord-308835-999kewdw	194	24	synthesized	synthesize	VBN
cord-308835-999kewdw	194	25	in	in	IN
cord-308835-999kewdw	194	26	JHMVinfected	jhmvinfected	JJ
cord-308835-999kewdw	194	27	cells	cell	NNS
cord-308835-999kewdw	194	28	as	as	IN
cord-308835-999kewdw	194	29	determined	determine	VBN
cord-308835-999kewdw	194	30	by	by	IN
cord-308835-999kewdw	194	31	the	the	DT
cord-308835-999kewdw	194	32	incorporation	incorporation	NN
cord-308835-999kewdw	194	33	of	of	IN
cord-308835-999kewdw	194	34	rSH]uridine	rsh]uridine	NN
cord-308835-999kewdw	194	35	or	or	CC
cord-308835-999kewdw	194	36	[	[	-LRB-
cord-308835-999kewdw	194	37	=	=	NFP
cord-308835-999kewdw	194	38	Plorthophosphate	Plorthophosphate	NNP
cord-308835-999kewdw	194	39	into	into	IN
cord-308835-999kewdw	194	40	acid	acid	NN
cord-308835-999kewdw	194	41	-	-	HYPH
cord-308835-999kewdw	194	42	insoluble	insoluble	JJ
cord-308835-999kewdw	194	43	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	194	44	material	material	NN
cord-308835-999kewdw	194	45	in	in	IN
cord-308835-999kewdw	194	46	the	the	DT
cord-308835-999kewdw	194	47	presence	presence	NN
cord-308835-999kewdw	194	48	of	of	IN
cord-308835-999kewdw	194	49	actinomycin	actinomycin	NNS
cord-308835-999kewdw	194	50	D.	D.	NNP
cord-308835-999kewdw	195	1	The	the	DT
cord-308835-999kewdw	195	2	Molecular	Molecular	NNP
cord-308835-999kewdw	195	3	Weights	Weights	NNPS
cord-308835-999kewdw	195	4	of	of	IN
cord-308835-999kewdw	195	5	MHV	MHV	NNP
cord-308835-999kewdw	195	6	-	-	HYPH
cord-308835-999kewdw	195	7	Specific	Specific	NNP
cord-308835-999kewdw	195	8	Intracellular	Intracellular	NNP
cord-308835-999kewdw	195	9	RNA	RNA	NNP
cord-308835-999kewdw	196	1	The	the	DT
cord-308835-999kewdw	196	2	molecular	molecular	JJ
cord-308835-999kewdw	196	3	weights	weight	NNS
cord-308835-999kewdw	196	4	of	of	IN
cord-308835-999kewdw	196	5	MHV	mhv	NN
cord-308835-999kewdw	196	6	-	-	HYPH
cord-308835-999kewdw	196	7	specific	specific	JJ
cord-308835-999kewdw	196	8	RNA	RNA	NNP
cord-308835-999kewdw	196	9	species	specie	NNS
cord-308835-999kewdw	196	10	l-7	l-7	NN
cord-308835-999kewdw	196	11	were	be	VBD
cord-308835-999kewdw	196	12	determined	determine	VBN
cord-308835-999kewdw	196	13	by	by	IN
cord-308835-999kewdw	196	14	agarose	agarose	JJ
cord-308835-999kewdw	196	15	gel	gel	NN
cord-308835-999kewdw	196	16	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	196	17	with	with	IN
cord-308835-999kewdw	196	18	appropriate	appropriate	JJ
cord-308835-999kewdw	196	19	molecular	molecular	JJ
cord-308835-999kewdw	196	20	weight	weight	NN
cord-308835-999kewdw	196	21	markers	marker	NNS
cord-308835-999kewdw	196	22	.	.	.
cord-308835-999kewdw	197	1	Glyoxal	Glyoxal	NNP
cord-308835-999kewdw	197	2	denaturation	denaturation	NN
cord-308835-999kewdw	197	3	allows	allow	VBZ
cord-308835-999kewdw	197	4	the	the	DT
cord-308835-999kewdw	197	5	use	use	NN
cord-308835-999kewdw	197	6	of	of	IN
cord-308835-999kewdw	197	7	DNA	DNA	NNP
cord-308835-999kewdw	197	8	as	as	RB
cord-308835-999kewdw	197	9	well	well	RB
cord-308835-999kewdw	197	10	as	as	IN
cord-308835-999kewdw	197	11	RNA	rna	NN
cord-308835-999kewdw	197	12	size	size	NN
cord-308835-999kewdw	197	13	markers	marker	NNS
cord-308835-999kewdw	197	14	(	(	-LRB-
cord-308835-999kewdw	197	15	McMaster	McMaster	NNP
cord-308835-999kewdw	197	16	and	and	CC
cord-308835-999kewdw	197	17	Carmichael	Carmichael	NNP
cord-308835-999kewdw	197	18	,	,	,
cord-308835-999kewdw	197	19	1977	1977	CD
cord-308835-999kewdw	197	20	)	)	-RRB-
cord-308835-999kewdw	197	21	.	.	.
cord-308835-999kewdw	198	1	A	a	DT
cord-308835-999kewdw	198	2	plot	plot	NN
cord-308835-999kewdw	198	3	of	of	IN
cord-308835-999kewdw	198	4	the	the	DT
cord-308835-999kewdw	198	5	log	log	NN
cord-308835-999kewdw	198	6	,	,	,
cord-308835-999kewdw	198	7	,	,	,
cord-308835-999kewdw	198	8	molecular	molecular	JJ
cord-308835-999kewdw	198	9	weight	weight	NN
cord-308835-999kewdw	198	10	against	against	IN
cord-308835-999kewdw	198	11	electrophoretic	electrophoretic	JJ
cord-308835-999kewdw	198	12	mobility	mobility	NN
cord-308835-999kewdw	198	13	is	be	VBZ
cord-308835-999kewdw	198	14	shown	show	VBN
cord-308835-999kewdw	198	15	in	in	IN
cord-308835-999kewdw	198	16	Fig	Fig	NNP
cord-308835-999kewdw	198	17	.	.	.
cord-308835-999kewdw	199	1	3	3	LS
cord-308835-999kewdw	199	2	.	.	.
cord-308835-999kewdw	200	1	A	a	DT
cord-308835-999kewdw	200	2	linear	linear	JJ
cord-308835-999kewdw	200	3	relationship	relationship	NN
cord-308835-999kewdw	200	4	was	be	VBD
cord-308835-999kewdw	200	5	obtained	obtain	VBN
cord-308835-999kewdw	200	6	over	over	IN
cord-308835-999kewdw	200	7	a	a	DT
cord-308835-999kewdw	200	8	molecular	molecular	JJ
cord-308835-999kewdw	200	9	weight	weight	NN
cord-308835-999kewdw	200	10	range	range	NN
cord-308835-999kewdw	200	11	of	of	IN
cord-308835-999kewdw	200	12	5.5	5.5	CD
cord-308835-999kewdw	200	13	X	x	NN
cord-308835-999kewdw	200	14	105	105	CD
cord-308835-999kewdw	200	15	-	-	HYPH
cord-308835-999kewdw	200	16	1.15	1.15	CD
cord-308835-999kewdw	200	17	X	X	NNS
cord-308835-999kewdw	200	18	107	107	CD
cord-308835-999kewdw	200	19	.	.	.
cord-308835-999kewdw	201	1	The	the	DT
cord-308835-999kewdw	201	2	molecular	molecular	JJ
cord-308835-999kewdw	201	3	weights	weight	NNS
cord-308835-999kewdw	201	4	obtained	obtain	VBN
cord-308835-999kewdw	201	5	for	for	IN
cord-308835-999kewdw	201	6	the	the	DT
cord-308835-999kewdw	201	7	seven	seven	CD
cord-308835-999kewdw	201	8	major	major	JJ
cord-308835-999kewdw	201	9	MHV	MHV	NNP
cord-308835-999kewdw	201	10	specific	specific	JJ
cord-308835-999kewdw	201	11	RNAs	rna	NNS
cord-308835-999kewdw	201	12	are	be	VBP
cord-308835-999kewdw	201	13	shown	show	VBN
cord-308835-999kewdw	201	14	in	in	IN
cord-308835-999kewdw	201	15	Table	Table	NNP
cord-308835-999kewdw	201	16	1	1	CD
cord-308835-999kewdw	201	17	.	.	.
cord-308835-999kewdw	202	1	Cytoplasmic	Cytoplasmic	NNP
cord-308835-999kewdw	202	2	RNA	RNA	NNP
cord-308835-999kewdw	202	3	was	be	VBD
cord-308835-999kewdw	202	4	extracted	extract	VBN
cord-308835-999kewdw	202	5	from	from	IN
cord-308835-999kewdw	202	6	infected	infected	JJ
cord-308835-999kewdw	202	7	cells	cell	NNS
cord-308835-999kewdw	202	8	labeled	label	VBN
cord-308835-999kewdw	202	9	from	from	IN
cord-308835-999kewdw	202	10	4	4	CD
cord-308835-999kewdw	202	11	to	to	TO
cord-308835-999kewdw	202	12	8	8	CD
cord-308835-999kewdw	202	13	hpi	hpi	NNS
cord-308835-999kewdw	202	14	and	and	CC
cord-308835-999kewdw	202	15	analyzed	analyze	VBN
cord-308835-999kewdw	202	16	by	by	IN
cord-308835-999kewdw	202	17	agarose	agarose	JJ
cord-308835-999kewdw	202	18	gel	gel	NN
cord-308835-999kewdw	202	19	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	202	20	.	.	.
cord-308835-999kewdw	203	1	The	the	DT
cord-308835-999kewdw	203	2	relative	relative	JJ
cord-308835-999kewdw	203	3	molar	molar	JJ
cord-308835-999kewdw	203	4	amounts	amount	NNS
cord-308835-999kewdw	203	5	of	of	IN
cord-308835-999kewdw	203	6	the	the	DT
cord-308835-999kewdw	203	7	individual	individual	JJ
cord-308835-999kewdw	203	8	MHV	mhv	NN
cord-308835-999kewdw	203	9	-	-	HYPH
cord-308835-999kewdw	203	10	specific	specific	JJ
cord-308835-999kewdw	203	11	RNAs	rna	NNS
cord-308835-999kewdw	203	12	were	be	VBD
cord-308835-999kewdw	203	13	quantitated	quantitate	VBN
cord-308835-999kewdw	203	14	as	as	IN
cord-308835-999kewdw	203	15	described	describe	VBN
cord-308835-999kewdw	203	16	under	under	IN
cord-308835-999kewdw	203	17	Materials	material	NNS
cord-308835-999kewdw	203	18	and	and	CC
cord-308835-999kewdw	203	19	Methods	method	NNS
cord-308835-999kewdw	203	20	.	.	.
cord-308835-999kewdw	204	1	The	the	DT
cord-308835-999kewdw	204	2	results	result	NNS
cord-308835-999kewdw	204	3	are	be	VBP
cord-308835-999kewdw	204	4	shown	show	VBN
cord-308835-999kewdw	204	5	in	in	IN
cord-308835-999kewdw	204	6	Table	Table	NNP
cord-308835-999kewdw	204	7	 	 	_SP
cord-308835-999kewdw	204	8	1	1	CD
cord-308835-999kewdw	204	9	.	.	.
cord-308835-999kewdw	205	1	A59V	A59V	NNP
cord-308835-999kewdw	205	2	-	-	:
cord-308835-999kewdw	205	3	and	and	CC
cord-308835-999kewdw	205	4	JHMV	JHMV	NNP
cord-308835-999kewdw	205	5	-	-	HYPH
cord-308835-999kewdw	205	6	infected	infect	VBN
cord-308835-999kewdw	205	7	cells	cell	NNS
cord-308835-999kewdw	205	8	synthesize	synthesize	VBP
cord-308835-999kewdw	205	9	virus	virus	NN
cord-308835-999kewdw	205	10	-	-	HYPH
cord-308835-999kewdw	205	11	specific	specific	JJ
cord-308835-999kewdw	205	12	RNA	RNA	NNP
cord-308835-999kewdw	205	13	species	specie	NNS
cord-308835-999kewdw	205	14	in	in	IN
cord-308835-999kewdw	205	15	similar	similar	JJ
cord-308835-999kewdw	205	16	ratios	ratio	NNS
cord-308835-999kewdw	205	17	with	with	IN
cord-308835-999kewdw	205	18	one	one	CD
cord-308835-999kewdw	205	19	exception	exception	NN
cord-308835-999kewdw	205	20	.	.	.
cord-308835-999kewdw	206	1	The	the	DT
cord-308835-999kewdw	206	2	relative	relative	JJ
cord-308835-999kewdw	206	3	amount	amount	NN
cord-308835-999kewdw	206	4	of	of	IN
cord-308835-999kewdw	206	5	RNA5	RNA5	NNP
cord-308835-999kewdw	206	6	synthesized	synthesize	VBN
cord-308835-999kewdw	206	7	in	in	IN
cord-308835-999kewdw	206	8	JHMVinfected	jhmvinfected	JJ
cord-308835-999kewdw	206	9	cells	cell	NNS
cord-308835-999kewdw	206	10	is	be	VBZ
cord-308835-999kewdw	206	11	about	about	RB
cord-308835-999kewdw	206	12	one	one	CD
cord-308835-999kewdw	206	13	-	-	HYPH
cord-308835-999kewdw	206	14	tenth	tenth	NN
cord-308835-999kewdw	206	15	of	of	IN
cord-308835-999kewdw	206	16	that	that	DT
cord-308835-999kewdw	206	17	synthesized	synthesize	VBN
cord-308835-999kewdw	206	18	in	in	IN
cord-308835-999kewdw	206	19	cells	cell	NNS
cord-308835-999kewdw	206	20	infected	infect	VBN
cord-308835-999kewdw	206	21	with	with	IN
cord-308835-999kewdw	206	22	A59V.	a59v.	NN
cord-308835-999kewdw	207	1	This	this	DT
cord-308835-999kewdw	207	2	was	be	VBD
cord-308835-999kewdw	207	3	a	a	DT
cord-308835-999kewdw	207	4	consistent	consistent	JJ
cord-308835-999kewdw	207	5	finding	finding	NN
cord-308835-999kewdw	207	6	in	in	IN
cord-308835-999kewdw	207	7	multiple	multiple	JJ
cord-308835-999kewdw	207	8	experiments	experiment	NNS
cord-308835-999kewdw	207	9	.	.	.
cord-308835-999kewdw	208	1	To	to	TO
cord-308835-999kewdw	208	2	determine	determine	VB
cord-308835-999kewdw	208	3	if	if	IN
cord-308835-999kewdw	208	4	there	there	EX
cord-308835-999kewdw	208	5	is	be	VBZ
cord-308835-999kewdw	208	6	temporal	temporal	JJ
cord-308835-999kewdw	208	7	regulation	regulation	NN
cord-308835-999kewdw	208	8	of	of	IN
cord-308835-999kewdw	208	9	MHV	mhv	NN
cord-308835-999kewdw	208	10	-	-	HYPH
cord-308835-999kewdw	208	11	specific	specific	JJ
cord-308835-999kewdw	208	12	RNA	RNA	NNP
cord-308835-999kewdw	208	13	synthesis	synthesis	NN
cord-308835-999kewdw	208	14	,	,	,
cord-308835-999kewdw	208	15	replicate	replicate	VB
cord-308835-999kewdw	208	16	cultures	culture	NNS
cord-308835-999kewdw	208	17	of	of	IN
cord-308835-999kewdw	208	18	A59V	a59v	NN
cord-308835-999kewdw	208	19	,	,	,
cord-308835-999kewdw	208	20	JHMV	JHMV	NNP
cord-308835-999kewdw	208	21	,	,	,
cord-308835-999kewdw	208	22	or	or	CC
cord-308835-999kewdw	208	23	mock	mock	JJ
cord-308835-999kewdw	208	24	-	-	HYPH
cord-308835-999kewdw	208	25	infected	infect	VBN
cord-308835-999kewdw	208	26	cells	cell	NNS
cord-308835-999kewdw	208	27	were	be	VBD
cord-308835-999kewdw	208	28	pulse	pulse	NN
cord-308835-999kewdw	208	29	labeled	label	VBN
cord-308835-999kewdw	208	30	for	for	IN
cord-308835-999kewdw	208	31	1	1	CD
cord-308835-999kewdw	208	32	hr	hr	NN
cord-308835-999kewdw	208	33	at	at	IN
cord-308835-999kewdw	208	34	hourly	hourly	JJ
cord-308835-999kewdw	208	35	intervals	interval	NNS
cord-308835-999kewdw	208	36	and	and	CC
cord-308835-999kewdw	208	37	the	the	DT
cord-308835-999kewdw	208	38	intracellular	intracellular	JJ
cord-308835-999kewdw	208	39	RNA	RNA	NNP
cord-308835-999kewdw	208	40	was	be	VBD
cord-308835-999kewdw	208	41	extracted	extract	VBN
cord-308835-999kewdw	208	42	and	and	CC
cord-308835-999kewdw	208	43	analyzed	analyze	VBN
cord-308835-999kewdw	208	44	by	by	IN
cord-308835-999kewdw	208	45	gel	gel	NN
cord-308835-999kewdw	208	46	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	208	47	.	.	.
cord-308835-999kewdw	209	1	The	the	DT
cord-308835-999kewdw	209	2	results	result	NNS
cord-308835-999kewdw	209	3	for	for	IN
cord-308835-999kewdw	209	4	A59Vinfected	a59vinfected	JJ
cord-308835-999kewdw	209	5	cells	cell	NNS
cord-308835-999kewdw	209	6	are	be	VBP
cord-308835-999kewdw	209	7	shown	show	VBN
cord-308835-999kewdw	209	8	in	in	IN
cord-308835-999kewdw	209	9	Fig	Fig	NNP
cord-308835-999kewdw	209	10	.	.	.
cord-308835-999kewdw	210	1	4	4	LS
cord-308835-999kewdw	210	2	.	.	.
cord-308835-999kewdw	211	1	Virusspecific	Virusspecific	NNP
cord-308835-999kewdw	211	2	RNA	RNA	NNP
cord-308835-999kewdw	211	3	synthesis	synthesis	NN
cord-308835-999kewdw	211	4	was	be	VBD
cord-308835-999kewdw	211	5	barely	barely	RB
cord-308835-999kewdw	211	6	detectable	detectable	JJ
cord-308835-999kewdw	211	7	in	in	IN
cord-308835-999kewdw	211	8	infected	infected	JJ
cord-308835-999kewdw	211	9	cells	cell	NNS
cord-308835-999kewdw	211	10	labeled	label	VBN
cord-308835-999kewdw	211	11	from	from	IN
cord-308835-999kewdw	211	12	3	3	CD
cord-308835-999kewdw	211	13	to	to	IN
cord-308835-999kewdw	211	14	4	4	CD
cord-308835-999kewdw	211	15	hpi	hpi	NNP
cord-308835-999kewdw	211	16	.	.	.
cord-308835-999kewdw	212	1	RNAs	rna	NNS
cord-308835-999kewdw	212	2	4,5,6	4,5,6	CD
cord-308835-999kewdw	212	3	,	,	,
cord-308835-999kewdw	212	4	and	and	CC
cord-308835-999kewdw	212	5	7	7	CD
cord-308835-999kewdw	212	6	were	be	VBD
cord-308835-999kewdw	212	7	the	the	DT
cord-308835-999kewdw	212	8	only	only	JJ
cord-308835-999kewdw	212	9	spe	spe	NN
cord-308835-999kewdw	212	10	-	-	HYPH
cord-308835-999kewdw	212	11	ties	tie	NNS
cord-308835-999kewdw	212	12	present	present	JJ
cord-308835-999kewdw	212	13	in	in	IN
cord-308835-999kewdw	212	14	detectable	detectable	JJ
cord-308835-999kewdw	212	15	quantities	quantity	NNS
cord-308835-999kewdw	212	16	at	at	IN
cord-308835-999kewdw	212	17	this	this	DT
cord-308835-999kewdw	212	18	time	time	NN
cord-308835-999kewdw	212	19	.	.	.
cord-308835-999kewdw	213	1	At	at	IN
cord-308835-999kewdw	213	2	later	later	RB
cord-308835-999kewdw	213	3	times	time	NNS
cord-308835-999kewdw	213	4	all	all	DT
cord-308835-999kewdw	213	5	A59V	a59v	NN
cord-308835-999kewdw	213	6	-	-	HYPH
cord-308835-999kewdw	213	7	specific	specific	JJ
cord-308835-999kewdw	213	8	RNA	RNA	NNP
cord-308835-999kewdw	213	9	species	specie	NNS
cord-308835-999kewdw	213	10	were	be	VBD
cord-308835-999kewdw	213	11	synthesized	synthesize	VBN
cord-308835-999kewdw	213	12	coordinately	coordinately	RB
cord-308835-999kewdw	213	13	with	with	IN
cord-308835-999kewdw	213	14	respect	respect	NN
cord-308835-999kewdw	213	15	to	to	IN
cord-308835-999kewdw	213	16	time	time	NN
cord-308835-999kewdw	213	17	.	.	.
cord-308835-999kewdw	214	1	Virus	virus	NN
cord-308835-999kewdw	214	2	-	-	HYPH
cord-308835-999kewdw	214	3	specific	specific	JJ
cord-308835-999kewdw	214	4	RNA	RNA	NNP
cord-308835-999kewdw	214	5	synthesis	synthesis	NN
cord-308835-999kewdw	214	6	was	be	VBD
cord-308835-999kewdw	214	7	maximal	maximal	JJ
cord-308835-999kewdw	214	8	in	in	IN
cord-308835-999kewdw	214	9	this	this	DT
cord-308835-999kewdw	214	10	experiment	experiment	NN
cord-308835-999kewdw	214	11	at	at	IN
cord-308835-999kewdw	214	12	5	5	CD
cord-308835-999kewdw	214	13	-	-	SYM
cord-308835-999kewdw	214	14	6	6	CD
cord-308835-999kewdw	214	15	hr	hr	NN
cord-308835-999kewdw	214	16	and	and	CC
cord-308835-999kewdw	214	17	then	then	RB
cord-308835-999kewdw	214	18	declined	decline	VBD
cord-308835-999kewdw	214	19	.	.	.
cord-308835-999kewdw	215	1	The	the	DT
cord-308835-999kewdw	215	2	results	result	NNS
cord-308835-999kewdw	215	3	of	of	IN
cord-308835-999kewdw	215	4	a	a	DT
cord-308835-999kewdw	215	5	similar	similar	JJ
cord-308835-999kewdw	215	6	experiment	experiment	NN
cord-308835-999kewdw	215	7	with	with	IN
cord-308835-999kewdw	215	8	JHMV	JHMV	NNP
cord-308835-999kewdw	215	9	are	be	VBP
cord-308835-999kewdw	215	10	shown	show	VBN
cord-308835-999kewdw	215	11	in	in	IN
cord-308835-999kewdw	215	12	Fig	Fig	NNP
cord-308835-999kewdw	215	13	.	.	.
cord-308835-999kewdw	216	1	5	5	LS
cord-308835-999kewdw	216	2	.	.	.
cord-308835-999kewdw	217	1	Virus	virus	NN
cord-308835-999kewdw	217	2	-	-	HYPH
cord-308835-999kewdw	217	3	specific	specific	JJ
cord-308835-999kewdw	217	4	RNA	RNA	NNP
cord-308835-999kewdw	217	5	synthesis	synthesis	NN
cord-308835-999kewdw	217	6	was	be	VBD
cord-308835-999kewdw	217	7	first	first	RB
cord-308835-999kewdw	217	8	detected	detect	VBN
cord-308835-999kewdw	217	9	at	at	IN
cord-308835-999kewdw	217	10	4	4	CD
cord-308835-999kewdw	217	11	-	-	SYM
cord-308835-999kewdw	217	12	5	5	CD
cord-308835-999kewdw	217	13	hpi	hpi	NNP
cord-308835-999kewdw	217	14	.	.	.
cord-308835-999kewdw	218	1	At	at	IN
cord-308835-999kewdw	218	2	this	this	DT
cord-308835-999kewdw	218	3	time	time	NN
cord-308835-999kewdw	218	4	all	all	DT
cord-308835-999kewdw	218	5	RNA	RNA	NNP
cord-308835-999kewdw	218	6	species	specie	NNS
cord-308835-999kewdw	218	7	were	be	VBD
cord-308835-999kewdw	218	8	present	present	JJ
cord-308835-999kewdw	218	9	in	in	IN
cord-308835-999kewdw	218	10	detectable	detectable	JJ
cord-308835-999kewdw	218	11	amounts	amount	NNS
cord-308835-999kewdw	218	12	although	although	IN
cord-308835-999kewdw	218	13	only	only	RB
cord-308835-999kewdw	218	14	RNAs	rna	NNS
cord-308835-999kewdw	218	15	6	6	CD
cord-308835-999kewdw	218	16	and	and	CC
cord-308835-999kewdw	218	17	'	'	``
cord-308835-999kewdw	218	18	7	7	CD
cord-308835-999kewdw	218	19	are	be	VBP
cord-308835-999kewdw	218	20	visible	visible	JJ
cord-308835-999kewdw	218	21	in	in	IN
cord-308835-999kewdw	218	22	the	the	DT
cord-308835-999kewdw	218	23	photograph	photograph	NN
cord-308835-999kewdw	218	24	.	.	.
cord-308835-999kewdw	219	1	At	at	IN
cord-308835-999kewdw	219	2	later	later	RB
cord-308835-999kewdw	219	3	times	time	NNS
cord-308835-999kewdw	219	4	all	all	DT
cord-308835-999kewdw	219	5	of	of	IN
cord-308835-999kewdw	219	6	the	the	DT
cord-308835-999kewdw	219	7	JHMVspecific	JHMVspecific	NNP
cord-308835-999kewdw	219	8	RNA	RNA	NNP
cord-308835-999kewdw	219	9	species	specie	NNS
cord-308835-999kewdw	219	10	were	be	VBD
cord-308835-999kewdw	219	11	easily	easily	RB
cord-308835-999kewdw	219	12	detectable	detectable	JJ
cord-308835-999kewdw	219	13	and	and	CC
cord-308835-999kewdw	219	14	were	be	VBD
cord-308835-999kewdw	219	15	synthesized	synthesize	VBN
cord-308835-999kewdw	219	16	coordinately	coordinately	RB
cord-308835-999kewdw	219	17	.	.	.
cord-308835-999kewdw	220	1	RNA	RNA	NNP
cord-308835-999kewdw	220	2	extracted	extract	VBN
cord-308835-999kewdw	220	3	from	from	IN
cord-308835-999kewdw	220	4	A59V	A59V	NNP
cord-308835-999kewdw	220	5	,	,	,
cord-308835-999kewdw	220	6	JHMV	JHMV	NNP
cord-308835-999kewdw	220	7	,	,	,
cord-308835-999kewdw	220	8	and	and	CC
cord-308835-999kewdw	220	9	mock	mock	JJ
cord-308835-999kewdw	220	10	-	-	HYPH
cord-308835-999kewdw	220	11	infected	infect	VBN
cord-308835-999kewdw	220	12	cells	cell	NNS
cord-308835-999kewdw	220	13	was	be	VBD
cord-308835-999kewdw	220	14	fractionated	fractionate	VBN
cord-308835-999kewdw	220	15	by	by	IN
cord-308835-999kewdw	220	16	chromatography	chromatography	NN
cord-308835-999kewdw	220	17	over	over	IN
cord-308835-999kewdw	220	18	poly(U	poly(u	$
cord-308835-999kewdw	220	19	)	)	-RRB-
cord-308835-999kewdw	221	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	221	2	and	and	CC
cord-308835-999kewdw	221	3	then	then	RB
cord-308835-999kewdw	221	4	analyzed	analyze	VBN
cord-308835-999kewdw	221	5	by	by	IN
cord-308835-999kewdw	221	6	gel	gel	NN
cord-308835-999kewdw	221	7	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	221	8	(	(	-LRB-
cord-308835-999kewdw	221	9	Fig	Fig	NNP
cord-308835-999kewdw	221	10	.	.	.
cord-308835-999kewdw	221	11	6	6	CD
cord-308835-999kewdw	221	12	)	)	-RRB-
cord-308835-999kewdw	221	13	.	.	.
cord-308835-999kewdw	222	1	The	the	DT
cord-308835-999kewdw	222	2	seven	seven	CD
cord-308835-999kewdw	222	3	major	major	JJ
cord-308835-999kewdw	222	4	MHV	mhv	NN
cord-308835-999kewdw	222	5	-	-	HYPH
cord-308835-999kewdw	222	6	specific	specific	JJ
cord-308835-999kewdw	222	7	RNAs	rna	NNS
cord-308835-999kewdw	222	8	were	be	VBD
cord-308835-999kewdw	222	9	present	present	JJ
cord-308835-999kewdw	222	10	in	in	IN
cord-308835-999kewdw	222	11	the	the	DT
cord-308835-999kewdw	222	12	material	material	NN
cord-308835-999kewdw	222	13	which	which	WDT
cord-308835-999kewdw	222	14	bound	bind	VBN
cord-308835-999kewdw	222	15	to	to	IN
cord-308835-999kewdw	222	16	the	the	DT
cord-308835-999kewdw	222	17	poly(U	poly(u	NN
cord-308835-999kewdw	222	18	)	)	-RRB-
cord-308835-999kewdw	223	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	223	2	(	(	-LRB-
cord-308835-999kewdw	223	3	Pool	pool	NN
cord-308835-999kewdw	223	4	2	2	CD
cord-308835-999kewdw	223	5	)	)	-RRB-
cord-308835-999kewdw	223	6	.	.	.
cord-308835-999kewdw	224	1	In	in	IN
cord-308835-999kewdw	224	2	addition	addition	NN
cord-308835-999kewdw	224	3	,	,	,
cord-308835-999kewdw	224	4	the	the	DT
cord-308835-999kewdw	224	5	two	two	CD
cord-308835-999kewdw	224	6	minor	minor	JJ
cord-308835-999kewdw	224	7	MHV	mhv	NN
cord-308835-999kewdw	224	8	-	-	HYPH
cord-308835-999kewdw	224	9	specific	specific	JJ
cord-308835-999kewdw	224	10	RNA	RNA	NNP
cord-308835-999kewdw	224	11	species	specie	NNS
cord-308835-999kewdw	224	12	smaller	small	JJR
cord-308835-999kewdw	224	13	than	than	IN
cord-308835-999kewdw	224	14	RNA	RNA	NNP
cord-308835-999kewdw	224	15	7	7	CD
cord-308835-999kewdw	224	16	were	be	VBD
cord-308835-999kewdw	224	17	also	also	RB
cord-308835-999kewdw	224	18	present	present	JJ
cord-308835-999kewdw	224	19	in	in	IN
cord-308835-999kewdw	224	20	this	this	DT
cord-308835-999kewdw	224	21	pool	pool	NN
cord-308835-999kewdw	224	22	.	.	.
cord-308835-999kewdw	225	1	These	these	DT
cord-308835-999kewdw	225	2	RNAs	rna	NNS
cord-308835-999kewdw	225	3	are	be	VBP
cord-308835-999kewdw	225	4	therefore	therefore	RB
cord-308835-999kewdw	225	5	presumably	presumably	RB
cord-308835-999kewdw	225	6	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	225	7	.	.	.
cord-308835-999kewdw	226	1	That	that	IN
cord-308835-999kewdw	226	2	this	this	DT
cord-308835-999kewdw	226	3	is	be	VBZ
cord-308835-999kewdw	226	4	the	the	DT
cord-308835-999kewdw	226	5	case	case	NN
cord-308835-999kewdw	226	6	was	be	VBD
cord-308835-999kewdw	226	7	confirmed	confirm	VBN
cord-308835-999kewdw	226	8	by	by	IN
cord-308835-999kewdw	226	9	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	226	10	T1	t1	NN
cord-308835-999kewdw	226	11	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	226	12	(	(	-LRB-
cord-308835-999kewdw	226	13	see	see	VB
cord-308835-999kewdw	226	14	below	below	RB
cord-308835-999kewdw	226	15	)	)	-RRB-
cord-308835-999kewdw	226	16	.	.	.
cord-308835-999kewdw	227	1	The	the	DT
cord-308835-999kewdw	227	2	RNA	RNA	NNP
cord-308835-999kewdw	227	3	which	which	WDT
cord-308835-999kewdw	227	4	did	do	VBD
cord-308835-999kewdw	227	5	not	not	RB
cord-308835-999kewdw	227	6	bind	bind	VB
cord-308835-999kewdw	227	7	to	to	IN
cord-308835-999kewdw	227	8	poly(U	poly(u	ADD
cord-308835-999kewdw	227	9	)	)	-RRB-
cord-308835-999kewdw	228	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	228	2	(	(	-LRB-
cord-308835-999kewdw	228	3	Pool	pool	NN
cord-308835-999kewdw	228	4	1	1	CD
cord-308835-999kewdw	228	5	)	)	-RRB-
cord-308835-999kewdw	228	6	consists	consist	VBZ
cord-308835-999kewdw	228	7	of	of	IN
cord-308835-999kewdw	228	8	a	a	DT
cord-308835-999kewdw	228	9	heterogeneous	heterogeneous	JJ
cord-308835-999kewdw	228	10	mixture	mixture	NN
cord-308835-999kewdw	228	11	of	of	IN
cord-308835-999kewdw	228	12	RNA	RNA	NNP
cord-308835-999kewdw	228	13	species	specie	NNS
cord-308835-999kewdw	228	14	.	.	.
cord-308835-999kewdw	229	1	Discrete	discrete	JJ
cord-308835-999kewdw	229	2	bands	band	NNS
cord-308835-999kewdw	229	3	which	which	WDT
cord-308835-999kewdw	229	4	coelectrophoresed	coelectrophorese	VBD
cord-308835-999kewdw	229	5	with	with	IN
cord-308835-999kewdw	229	6	MHVspecific	MHVspecific	NNP
cord-308835-999kewdw	229	7	RNAs	rna	NNS
cord-308835-999kewdw	229	8	were	be	VBD
cord-308835-999kewdw	229	9	present	present	JJ
cord-308835-999kewdw	229	10	superimposed	superimpose	VBN
cord-308835-999kewdw	229	11	on	on	IN
cord-308835-999kewdw	229	12	a	a	DT
cord-308835-999kewdw	229	13	smear	smear	NN
cord-308835-999kewdw	229	14	of	of	IN
cord-308835-999kewdw	229	15	heterogeneous	heterogeneous	JJ
cord-308835-999kewdw	229	16	material	material	NN
cord-308835-999kewdw	229	17	.	.	.
cord-308835-999kewdw	230	1	The	the	DT
cord-308835-999kewdw	230	2	sum	sum	NN
cord-308835-999kewdw	230	3	of	of	IN
cord-308835-999kewdw	230	4	the	the	DT
cord-308835-999kewdw	230	5	molecular	molecular	JJ
cord-308835-999kewdw	230	6	weights	weight	NNS
cord-308835-999kewdw	230	7	of	of	IN
cord-308835-999kewdw	230	8	the	the	DT
cord-308835-999kewdw	230	9	six	six	CD
cord-308835-999kewdw	230	10	subgenomic	subgenomic	NNP
cord-308835-999kewdw	230	11	RNA	RNA	NNP
cord-308835-999kewdw	230	12	species	specie	NNS
cord-308835-999kewdw	230	13	exceeds	exceed	VBZ
cord-308835-999kewdw	230	14	the	the	DT
cord-308835-999kewdw	230	15	molecular	molecular	JJ
cord-308835-999kewdw	230	16	weight	weight	NN
cord-308835-999kewdw	230	17	of	of	IN
cord-308835-999kewdw	230	18	the	the	DT
cord-308835-999kewdw	230	19	genome	genome	NN
cord-308835-999kewdw	230	20	by	by	IN
cord-308835-999kewdw	230	21	approximately	approximately	RB
cord-308835-999kewdw	230	22	50	50	CD
cord-308835-999kewdw	230	23	%	%	NN
cord-308835-999kewdw	230	24	.	.	.
cord-308835-999kewdw	231	1	To	to	TO
cord-308835-999kewdw	231	2	investigate	investigate	VB
cord-308835-999kewdw	231	3	this	this	DT
cord-308835-999kewdw	231	4	observation	observation	NN
cord-308835-999kewdw	231	5	further	further	RB
cord-308835-999kewdw	231	6	,	,	,
cord-308835-999kewdw	231	7	the	the	DT
cord-308835-999kewdw	231	8	seven	seven	CD
cord-308835-999kewdw	231	9	major	major	JJ
cord-308835-999kewdw	231	10	MHVspecific	MHVspecific	NNP
cord-308835-999kewdw	231	11	RNAs	rna	NNS
cord-308835-999kewdw	231	12	were	be	VBD
cord-308835-999kewdw	231	13	purified	purify	VBN
cord-308835-999kewdw	231	14	and	and	CC
cord-308835-999kewdw	231	15	compared	compare	VBN
cord-308835-999kewdw	231	16	to	to	IN
cord-308835-999kewdw	231	17	each	each	DT
cord-308835-999kewdw	231	18	other	other	JJ
cord-308835-999kewdw	231	19	and	and	CC
cord-308835-999kewdw	231	20	to	to	IN
cord-308835-999kewdw	231	21	virion	virion	NN
cord-308835-999kewdw	231	22	RNA	RNA	NNP
cord-308835-999kewdw	231	23	using	use	VBG
cord-308835-999kewdw	231	24	the	the	DT
cord-308835-999kewdw	231	25	technique	technique	NN
cord-308835-999kewdw	231	26	of	of	IN
cord-308835-999kewdw	231	27	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	231	28	T1	t1	NN
cord-308835-999kewdw	231	29	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	231	30	.	.	.
cord-308835-999kewdw	232	1	The	the	DT
cord-308835-999kewdw	232	2	individual	individual	JJ
cord-308835-999kewdw	232	3	RNA	RNA	NNP
cord-308835-999kewdw	232	4	species	specie	NNS
cord-308835-999kewdw	232	5	and	and	CC
cord-308835-999kewdw	232	6	virion	virion	NN
cord-308835-999kewdw	232	7	RNA	RNA	NNP
cord-308835-999kewdw	232	8	were	be	VBD
cord-308835-999kewdw	232	9	purified	purify	VBN
cord-308835-999kewdw	232	10	from	from	IN
cord-308835-999kewdw	232	11	cells	cell	NNS
cord-308835-999kewdw	232	12	labeled	label	VBN
cord-308835-999kewdw	232	13	with	with	IN
cord-308835-999kewdw	232	14	[	[	-LRB-
cord-308835-999kewdw	232	15	32P]orthophosphate	32P]orthophosphate	NNP
cord-308835-999kewdw	232	16	as	as	IN
cord-308835-999kewdw	232	17	described	describe	VBN
cord-308835-999kewdw	232	18	under	under	IN
cord-308835-999kewdw	232	19	Materials	material	NNS
cord-308835-999kewdw	232	20	and	and	CC
cord-308835-999kewdw	232	21	Methods	method	NNS
cord-308835-999kewdw	232	22	.	.	.
cord-308835-999kewdw	233	1	The	the	DT
cord-308835-999kewdw	233	2	purified	purify	VBN
cord-308835-999kewdw	233	3	RNAs	rna	NNS
cord-308835-999kewdw	233	4	were	be	VBD
cord-308835-999kewdw	233	5	digested	digest	VBN
cord-308835-999kewdw	233	6	with	with	IN
cord-308835-999kewdw	233	7	ribonuclease	ribonuclease	NN
cord-308835-999kewdw	233	8	Tr	Tr	NNP
cord-308835-999kewdw	233	9	and	and	CC
cord-308835-999kewdw	233	10	the	the	DT
cord-308835-999kewdw	233	11	resulting	result	VBG
cord-308835-999kewdw	233	12	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	233	13	separated	separate	VBN
cord-308835-999kewdw	233	14	by	by	IN
cord-308835-999kewdw	233	15	two	two	CD
cord-308835-999kewdw	233	16	-	-	HYPH
cord-308835-999kewdw	233	17	dimensional	dimensional	JJ
cord-308835-999kewdw	233	18	gel	gel	NN
cord-308835-999kewdw	233	19	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	233	20	.	.	.
cord-308835-999kewdw	234	1	The	the	DT
cord-308835-999kewdw	234	2	results	result	NNS
cord-308835-999kewdw	234	3	are	be	VBP
cord-308835-999kewdw	234	4	shown	show	VBN
cord-308835-999kewdw	234	5	in	in	IN
cord-308835-999kewdw	234	6	Figs	Figs	NNPS
cord-308835-999kewdw	234	7	.	.	.
cord-308835-999kewdw	235	1	7	7	CD
cord-308835-999kewdw	235	2	and	and	CC
cord-308835-999kewdw	235	3	8	8	CD
cord-308835-999kewdw	235	4	.	.	.
cord-308835-999kewdw	236	1	Poly(A	Poly(A	NNP
cord-308835-999kewdw	236	2	)	)	-RRB-
cord-308835-999kewdw	236	3	tracts	tract	NNS
cord-308835-999kewdw	236	4	are	be	VBP
cord-308835-999kewdw	236	5	seen	see	VBN
cord-308835-999kewdw	236	6	as	as	IN
cord-308835-999kewdw	236	7	streaks	streak	NNS
cord-308835-999kewdw	236	8	in	in	IN
cord-308835-999kewdw	236	9	the	the	DT
cord-308835-999kewdw	236	10	upper	upper	JJ
cord-308835-999kewdw	236	11	left	left	JJ
cord-308835-999kewdw	236	12	hand	hand	NN
cord-308835-999kewdw	236	13	corner	corner	NN
cord-308835-999kewdw	236	14	of	of	IN
cord-308835-999kewdw	236	15	the	the	DT
cord-308835-999kewdw	236	16	fingerprints	fingerprint	NNS
cord-308835-999kewdw	236	17	of	of	IN
cord-308835-999kewdw	236	18	A59V	a59v	NN
cord-308835-999kewdw	236	19	and	and	CC
cord-308835-999kewdw	236	20	JHMV	JHMV	NNP
cord-308835-999kewdw	236	21	virion	virion	NN
cord-308835-999kewdw	236	22	RNAs	rna	NNS
cord-308835-999kewdw	236	23	.	.	.
cord-308835-999kewdw	237	1	This	this	DT
cord-308835-999kewdw	237	2	confirms	confirm	VBZ
cord-308835-999kewdw	237	3	the	the	DT
cord-308835-999kewdw	237	4	findings	finding	NNS
cord-308835-999kewdw	237	5	of	of	IN
cord-308835-999kewdw	237	6	others	other	NNS
cord-308835-999kewdw	237	7	(	(	-LRB-
cord-308835-999kewdw	237	8	Yogo	Yogo	NNP
cord-308835-999kewdw	237	9	et	et	NNP
cord-308835-999kewdw	237	10	al	al	NNP
cord-308835-999kewdw	237	11	.	.	NNP
cord-308835-999kewdw	237	12	,	,	,
cord-308835-999kewdw	237	13	1977	1977	CD
cord-308835-999kewdw	237	14	;	;	:
cord-308835-999kewdw	237	15	Lai	Lai	NNP
cord-308835-999kewdw	237	16	and	and	CC
cord-308835-999kewdw	237	17	Stohlman	Stohlman	NNP
cord-308835-999kewdw	237	18	,	,	,
cord-308835-999kewdw	237	19	1978	1978	CD
cord-308835-999kewdw	237	20	;	;	:
cord-308835-999kewdw	237	21	Wege	Wege	NNP
cord-308835-999kewdw	237	22	et	et	NNP
cord-308835-999kewdw	237	23	al	al	NNP
cord-308835-999kewdw	237	24	.	.	NNP
cord-308835-999kewdw	237	25	,	,	,
cord-308835-999kewdw	237	26	1978	1978	CD
cord-308835-999kewdw	237	27	)	)	-RRB-
cord-308835-999kewdw	237	28	that	that	IN
cord-308835-999kewdw	237	29	the	the	DT
cord-308835-999kewdw	237	30	MHV	MHV	NNP
cord-308835-999kewdw	237	31	genome	genome	NN
cord-308835-999kewdw	237	32	is	be	VBZ
cord-308835-999kewdw	237	33	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	237	34	.	.	.
cord-308835-999kewdw	238	1	The	the	DT
cord-308835-999kewdw	238	2	seven	seven	CD
cord-308835-999kewdw	238	3	major	major	JJ
cord-308835-999kewdw	238	4	A59V	a59v	NN
cord-308835-999kewdw	238	5	-	-	:
cord-308835-999kewdw	238	6	and	and	CC
cord-308835-999kewdw	238	7	JHMV	JHMV	NNP
cord-308835-999kewdw	238	8	-	-	HYPH
cord-308835-999kewdw	238	9	specific	specific	JJ
cord-308835-999kewdw	238	10	intracellular	intracellular	JJ
cord-308835-999kewdw	238	11	RNAs	rna	NNS
cord-308835-999kewdw	238	12	are	be	VBP
cord-308835-999kewdw	238	13	also	also	RB
cord-308835-999kewdw	238	14	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	238	15	.	.	.
cord-308835-999kewdw	239	1	A	a	DT
cord-308835-999kewdw	239	2	comparison	comparison	NN
cord-308835-999kewdw	239	3	of	of	IN
cord-308835-999kewdw	239	4	the	the	DT
cord-308835-999kewdw	239	5	fingerprints	fingerprint	NNS
cord-308835-999kewdw	239	6	of	of	IN
cord-308835-999kewdw	239	7	the	the	DT
cord-308835-999kewdw	239	8	seven	seven	CD
cord-308835-999kewdw	239	9	A59V	a59v	NN
cord-308835-999kewdw	239	10	-	-	HYPH
cord-308835-999kewdw	239	11	specific	specific	JJ
cord-308835-999kewdw	239	12	intracellular	intracellular	JJ
cord-308835-999kewdw	239	13	RNAs	rna	NNS
cord-308835-999kewdw	239	14	with	with	IN
cord-308835-999kewdw	239	15	each	each	DT
cord-308835-999kewdw	239	16	other	other	JJ
cord-308835-999kewdw	239	17	and	and	CC
cord-308835-999kewdw	239	18	with	with	IN
cord-308835-999kewdw	239	19	A59V	A59V	NNP
cord-308835-999kewdw	239	20	virion	virion	NN
cord-308835-999kewdw	239	21	RNA	RNA	NNP
cord-308835-999kewdw	239	22	reveals	reveal	VBZ
cord-308835-999kewdw	239	23	several	several	JJ
cord-308835-999kewdw	239	24	things	thing	NNS
cord-308835-999kewdw	239	25	.	.	.
cord-308835-999kewdw	240	1	The	the	DT
cord-308835-999kewdw	240	2	fingerprint	fingerprint	NN
cord-308835-999kewdw	240	3	of	of	IN
cord-308835-999kewdw	240	4	the	the	DT
cord-308835-999kewdw	240	5	largest	large	JJS
cord-308835-999kewdw	240	6	intracellular	intracellular	JJ
cord-308835-999kewdw	240	7	RNA	RNA	NNP
cord-308835-999kewdw	240	8	,	,	,
cord-308835-999kewdw	240	9	species	species	NN
cord-308835-999kewdw	240	10	1	1	CD
cord-308835-999kewdw	240	11	,	,	,
cord-308835-999kewdw	240	12	is	be	VBZ
cord-308835-999kewdw	240	13	almost	almost	RB
cord-308835-999kewdw	240	14	identical	identical	JJ
cord-308835-999kewdw	240	15	to	to	IN
cord-308835-999kewdw	240	16	that	that	DT
cord-308835-999kewdw	240	17	of	of	IN
cord-308835-999kewdw	240	18	virion	virion	NN
cord-308835-999kewdw	240	19	RNA	RNA	NNP
cord-308835-999kewdw	240	20	.	.	.
cord-308835-999kewdw	241	1	There	there	EX
cord-308835-999kewdw	241	2	are	be	VBP
cord-308835-999kewdw	241	3	,	,	,
cord-308835-999kewdw	241	4	however	however	RB
cord-308835-999kewdw	241	5	,	,	,
cord-308835-999kewdw	241	6	several	several	JJ
cord-308835-999kewdw	241	7	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	241	8	which	which	WDT
cord-308835-999kewdw	241	9	are	be	VBP
cord-308835-999kewdw	241	10	underrepresented	underrepresente	VBN
cord-308835-999kewdw	241	11	in	in	IN
cord-308835-999kewdw	241	12	the	the	DT
cord-308835-999kewdw	241	13	fingerprint	fingerprint	NN
cord-308835-999kewdw	241	14	of	of	IN
cord-308835-999kewdw	241	15	RNA1	RNA1	NNP
cord-308835-999kewdw	241	16	as	as	IN
cord-308835-999kewdw	241	17	com-	com-	NN
cord-308835-999kewdw	242	1	FIG	FIG	NNP
cord-308835-999kewdw	242	2	.	.	.
cord-308835-999kewdw	243	1	4	4	LS
cord-308835-999kewdw	243	2	.	.	.
cord-308835-999kewdw	244	1	The	the	DT
cord-308835-999kewdw	244	2	kinetics	kinetic	NNS
cord-308835-999kewdw	244	3	of	of	IN
cord-308835-999kewdw	244	4	A59V	a59v	NN
cord-308835-999kewdw	244	5	-	-	HYPH
cord-308835-999kewdw	244	6	specific	specific	JJ
cord-308835-999kewdw	244	7	RNA	RNA	NNP
cord-308835-999kewdw	244	8	synthesis	synthesis	NN
cord-308835-999kewdw	244	9	.	.	.
cord-308835-999kewdw	245	1	1'7CL.l	1'7cl.l	CD
cord-308835-999kewdw	245	2	cells	cell	NNS
cord-308835-999kewdw	245	3	were	be	VBD
cord-308835-999kewdw	245	4	infected	infect	VBN
cord-308835-999kewdw	245	5	with	with	IN
cord-308835-999kewdw	245	6	A59V	A59V	NNP
cord-308835-999kewdw	245	7	or	or	CC
cord-308835-999kewdw	245	8	mockinfected	mockinfecte	VBN
cord-308835-999kewdw	245	9	,	,	,
cord-308835-999kewdw	245	10	resuspended	resuspend	VBN
cord-308835-999kewdw	245	11	in	in	IN
cord-308835-999kewdw	245	12	DME2	DME2	NNP
cord-308835-999kewdw	245	13	,	,	,
cord-308835-999kewdw	245	14	and	and	CC
cord-308835-999kewdw	245	15	seeded	seed	VBN
cord-308835-999kewdw	245	16	in	in	IN
cord-308835-999kewdw	245	17	replicate	replicate	VB
cord-308835-999kewdw	245	18	60-mm	60-mm	CD
cord-308835-999kewdw	245	19	dishes	dish	NNS
cord-308835-999kewdw	245	20	and	and	CC
cord-308835-999kewdw	245	21	incubated	incubate	VBN
cord-308835-999kewdw	245	22	at	at	IN
cord-308835-999kewdw	245	23	37	37	CD
cord-308835-999kewdw	245	24	"	"	''
cord-308835-999kewdw	245	25	.	.	.
cord-308835-999kewdw	246	1	At	at	IN
cord-308835-999kewdw	246	2	1-hr	1-hr	CD
cord-308835-999kewdw	246	3	intervals	interval	NNS
cord-308835-999kewdw	246	4	individual	individual	JJ
cord-308835-999kewdw	246	5	dishes	dish	NNS
cord-308835-999kewdw	246	6	were	be	VBD
cord-308835-999kewdw	246	7	removed	remove	VBN
cord-308835-999kewdw	246	8	from	from	IN
cord-308835-999kewdw	246	9	the	the	DT
cord-308835-999kewdw	246	10	incubator	incubator	NN
cord-308835-999kewdw	246	11	,	,	,
cord-308835-999kewdw	246	12	media	medium	NNS
cord-308835-999kewdw	246	13	removed	remove	VBN
cord-308835-999kewdw	246	14	,	,	,
cord-308835-999kewdw	246	15	washed	wash	VBD
cord-308835-999kewdw	246	16	twice	twice	RB
cord-308835-999kewdw	246	17	with	with	IN
cord-308835-999kewdw	246	18	phosphate	phosphate	NN
cord-308835-999kewdw	246	19	-	-	HYPH
cord-308835-999kewdw	246	20	free	free	JJ
cord-308835-999kewdw	246	21	DMEL	dmel	NN
cord-308835-999kewdw	246	22	,	,	,
cord-308835-999kewdw	246	23	and	and	CC
cord-308835-999kewdw	246	24	then	then	RB
cord-308835-999kewdw	246	25	incubated	incubate	VBD
cord-308835-999kewdw	246	26	for	for	IN
cord-308835-999kewdw	246	27	15	15	CD
cord-308835-999kewdw	246	28	min	min	NN
cord-308835-999kewdw	246	29	in	in	IN
cord-308835-999kewdw	246	30	phosphate	phosphate	NN
cord-308835-999kewdw	246	31	-	-	HYPH
cord-308835-999kewdw	246	32	free	free	JJ
cord-308835-999kewdw	246	33	DME2	DME2	NNP
cord-308835-999kewdw	246	34	containing	contain	VBG
cord-308835-999kewdw	246	35	5	5	CD
cord-308835-999kewdw	246	36	pg	pg	NN
cord-308835-999kewdw	246	37	/	/	SYM
cord-308835-999kewdw	246	38	ml	ml	IN
cord-308835-999kewdw	246	39	actinomycin	actinomycin	NNS
cord-308835-999kewdw	246	40	D.	D.	NNP
cord-308835-999kewdw	247	1	The	the	DT
cord-308835-999kewdw	247	2	cells	cell	NNS
cord-308835-999kewdw	247	3	were	be	VBD
cord-308835-999kewdw	247	4	then	then	RB
cord-308835-999kewdw	247	5	labeled	label	VBN
cord-308835-999kewdw	247	6	for	for	IN
cord-308835-999kewdw	247	7	1	1	CD
cord-308835-999kewdw	247	8	hr	hr	NN
cord-308835-999kewdw	247	9	with	with	IN
cord-308835-999kewdw	247	10	250	250	CD
cord-308835-999kewdw	247	11	&	&	CC
cord-308835-999kewdw	247	12	i	i	NNP
cord-308835-999kewdw	247	13	/	/	SYM
cord-308835-999kewdw	247	14	ml	ml	NNP
cord-308835-999kewdw	248	1	[	[	-LRB-
cord-308835-999kewdw	248	2	aaP]orthophosphate	aap]orthophosphate	NN
cord-308835-999kewdw	248	3	and	and	CC
cord-308835-999kewdw	248	4	the	the	DT
cord-308835-999kewdw	248	5	cytoplasmic	cytoplasmic	JJ
cord-308835-999kewdw	248	6	nucleic	nucleic	JJ
cord-308835-999kewdw	248	7	acids	acid	NNS
cord-308835-999kewdw	248	8	were	be	VBD
cord-308835-999kewdw	248	9	then	then	RB
cord-308835-999kewdw	248	10	extracted	extract	VBN
cord-308835-999kewdw	248	11	and	and	CC
cord-308835-999kewdw	248	12	precipitated	precipitate	VBN
cord-308835-999kewdw	248	13	with	with	IN
cord-308835-999kewdw	248	14	ethanol	ethanol	NN
cord-308835-999kewdw	248	15	.	.	.
cord-308835-999kewdw	249	1	The	the	DT
cord-308835-999kewdw	249	2	RNA	RNA	NNP
cord-308835-999kewdw	249	3	was	be	VBD
cord-308835-999kewdw	249	4	collected	collect	VBN
cord-308835-999kewdw	249	5	by	by	IN
cord-308835-999kewdw	249	6	centrifugation	centrifugation	NN
cord-308835-999kewdw	249	7	,	,	,
cord-308835-999kewdw	249	8	glyoxalated	glyoxalated	NNP
cord-308835-999kewdw	249	9	,	,	,
cord-308835-999kewdw	249	10	and	and	CC
cord-308835-999kewdw	249	11	electrophoresed	electrophorese	VBD
cord-308835-999kewdw	249	12	on	on	IN
cord-308835-999kewdw	249	13	a	a	DT
cord-308835-999kewdw	249	14	1	1	CD
cord-308835-999kewdw	249	15	%	%	NN
cord-308835-999kewdw	249	16	agarose	agarose	NN
cord-308835-999kewdw	249	17	gel	gel	NN
cord-308835-999kewdw	249	18	.	.	.
cord-308835-999kewdw	250	1	The	the	DT
cord-308835-999kewdw	250	2	times	time	NNS
cord-308835-999kewdw	250	3	of	of	IN
cord-308835-999kewdw	250	4	labeling	labeling	NN
cord-308835-999kewdw	250	5	are	be	VBP
cord-308835-999kewdw	250	6	indicated	indicate	VBN
cord-308835-999kewdw	250	7	in	in	IN
cord-308835-999kewdw	250	8	the	the	DT
cord-308835-999kewdw	250	9	figure	figure	NN
cord-308835-999kewdw	250	10	.	.	.
cord-308835-999kewdw	251	1	The	the	DT
cord-308835-999kewdw	251	2	lane	lane	NN
cord-308835-999kewdw	251	3	containing	contain	VBG
cord-308835-999kewdw	251	4	RNA	RNA	NNP
cord-308835-999kewdw	251	5	from	from	IN
cord-308835-999kewdw	251	6	mock	mock	JJ
cord-308835-999kewdw	251	7	-	-	HYPH
cord-308835-999kewdw	251	8	infected	infected	JJ
cord-308835-999kewdw	251	9	cells	cell	NNS
cord-308835-999kewdw	251	10	is	be	VBZ
cord-308835-999kewdw	251	11	labeled	label	VBN
cord-308835-999kewdw	251	12	M.	M.	NNP
cord-308835-999kewdw	251	13	Fig	Fig	NNP
cord-308835-999kewdw	251	14	.	.	.
cord-308835-999kewdw	251	15	 	 	_SP
cord-308835-999kewdw	252	1	7	7	LS
cord-308835-999kewdw	252	2	,	,	,
cord-308835-999kewdw	252	3	panel	panel	NN
cord-308835-999kewdw	252	4	l	l	NNP
cord-308835-999kewdw	252	5	)	)	-RRB-
cord-308835-999kewdw	252	6	,	,	,
cord-308835-999kewdw	252	7	as	as	RB
cord-308835-999kewdw	252	8	well	well	RB
cord-308835-999kewdw	252	9	as	as	IN
cord-308835-999kewdw	252	10	an	an	DT
cord-308835-999kewdw	252	11	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	252	12	which	which	WDT
cord-308835-999kewdw	252	13	is	be	VBZ
cord-308835-999kewdw	252	14	overrepresented	overrepresente	VBN
cord-308835-999kewdw	252	15	in	in	IN
cord-308835-999kewdw	252	16	the	the	DT
cord-308835-999kewdw	252	17	fingerprints	fingerprint	NNS
cord-308835-999kewdw	252	18	of	of	IN
cord-308835-999kewdw	252	19	RNA1	RNA1	NNP
cord-308835-999kewdw	252	20	(	(	-LRB-
cord-308835-999kewdw	252	21	closed	closed	JJ
cord-308835-999kewdw	252	22	triangle	triangle	NN
cord-308835-999kewdw	252	23	)	)	-RRB-
cord-308835-999kewdw	252	24	and	and	CC
cord-308835-999kewdw	252	25	RNAs	rna	NNS
cord-308835-999kewdw	252	26	6	6	CD
cord-308835-999kewdw	252	27	and	and	CC
cord-308835-999kewdw	252	28	7	7	CD
cord-308835-999kewdw	252	29	(	(	-LRB-
cord-308835-999kewdw	252	30	see	see	VB
cord-308835-999kewdw	252	31	below	below	RB
cord-308835-999kewdw	252	32	)	)	-RRB-
cord-308835-999kewdw	252	33	.	.	.
cord-308835-999kewdw	253	1	All	all	PDT
cord-308835-999kewdw	253	2	the	the	DT
cord-308835-999kewdw	253	3	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	253	4	contained	contain	VBN
cord-308835-999kewdw	253	5	in	in	IN
cord-308835-999kewdw	253	6	RNA	RNA	NNP
cord-308835-999kewdw	253	7	species	species	NN
cord-308835-999kewdw	253	8	2	2	CD
cord-308835-999kewdw	253	9	are	be	VBP
cord-308835-999kewdw	253	10	present	present	JJ
cord-308835-999kewdw	253	11	in	in	IN
cord-308835-999kewdw	253	12	RNA	RNA	NNP
cord-308835-999kewdw	253	13	species	species	NN
cord-308835-999kewdw	253	14	1	1	CD
cord-308835-999kewdw	253	15	and	and	CC
cord-308835-999kewdw	253	16	are	be	VBP
cord-308835-999kewdw	253	17	a	a	DT
cord-308835-999kewdw	253	18	subset	subset	NN
cord-308835-999kewdw	253	19	of	of	IN
cord-308835-999kewdw	253	20	these	these	DT
cord-308835-999kewdw	253	21	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	253	22	.	.	.
cord-308835-999kewdw	254	1	Similarly	similarly	RB
cord-308835-999kewdw	254	2	,	,	,
cord-308835-999kewdw	254	3	the	the	DT
cord-308835-999kewdw	254	4	fingerprint	fingerprint	NN
cord-308835-999kewdw	254	5	of	of	IN
cord-308835-999kewdw	254	6	RNA	RNA	NNP
cord-308835-999kewdw	254	7	species	species	NN
cord-308835-999kewdw	254	8	3	3	CD
cord-308835-999kewdw	254	9	is	be	VBZ
cord-308835-999kewdw	254	10	a	a	DT
cord-308835-999kewdw	254	11	subset	subset	NN
cord-308835-999kewdw	254	12	of	of	IN
cord-308835-999kewdw	254	13	the	the	DT
cord-308835-999kewdw	254	14	fingerprint	fingerprint	NN
cord-308835-999kewdw	254	15	of	of	IN
cord-308835-999kewdw	254	16	RNA	RNA	NNP
cord-308835-999kewdw	254	17	species	species	NN
cord-308835-999kewdw	254	18	2	2	CD
cord-308835-999kewdw	254	19	.	.	.
cord-308835-999kewdw	255	1	An	an	DT
cord-308835-999kewdw	255	2	examination	examination	NN
cord-308835-999kewdw	255	3	of	of	IN
cord-308835-999kewdw	255	4	the	the	DT
cord-308835-999kewdw	255	5	fingerprint	fingerprint	NN
cord-308835-999kewdw	255	6	of	of	IN
cord-308835-999kewdw	255	7	RNA	RNA	NNP
cord-308835-999kewdw	255	8	species	specie	NNS
cord-308835-999kewdw	255	9	4	4	CD
cord-308835-999kewdw	255	10	reveals	reveal	VBZ
cord-308835-999kewdw	255	11	a	a	DT
cord-308835-999kewdw	255	12	new	new	JJ
cord-308835-999kewdw	255	13	spot	spot	NN
cord-308835-999kewdw	255	14	which	which	WDT
cord-308835-999kewdw	255	15	is	be	VBZ
cord-308835-999kewdw	255	16	not	not	RB
cord-308835-999kewdw	255	17	present	present	JJ
cord-308835-999kewdw	255	18	in	in	IN
cord-308835-999kewdw	255	19	any	any	DT
cord-308835-999kewdw	255	20	of	of	IN
cord-308835-999kewdw	255	21	the	the	DT
cord-308835-999kewdw	255	22	other	other	JJ
cord-308835-999kewdw	255	23	A59V	a59v	NN
cord-308835-999kewdw	255	24	-	-	HYPH
cord-308835-999kewdw	255	25	specific	specific	JJ
cord-308835-999kewdw	255	26	RNAs	rna	NNS
cord-308835-999kewdw	255	27	(	(	-LRB-
cord-308835-999kewdw	255	28	arrow	arrow	NN
cord-308835-999kewdw	255	29	in	in	IN
cord-308835-999kewdw	255	30	Fig	Fig	NNP
cord-308835-999kewdw	255	31	.	.	NNP
cord-308835-999kewdw	255	32	7	7	CD
cord-308835-999kewdw	255	33	,	,	,
cord-308835-999kewdw	255	34	panel	panel	NN
cord-308835-999kewdw	255	35	4	4	CD
cord-308835-999kewdw	255	36	)	)	-RRB-
cord-308835-999kewdw	255	37	.	.	.
cord-308835-999kewdw	256	1	The	the	DT
cord-308835-999kewdw	256	2	remaining	remain	VBG
cord-308835-999kewdw	256	3	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	256	4	observed	observe	VBN
cord-308835-999kewdw	256	5	in	in	IN
cord-308835-999kewdw	256	6	RNA	RNA	NNP
cord-308835-999kewdw	256	7	species	specie	NNS
cord-308835-999kewdw	256	8	4	4	CD
cord-308835-999kewdw	256	9	are	be	VBP
cord-308835-999kewdw	256	10	a	a	DT
cord-308835-999kewdw	256	11	subset	subset	NN
cord-308835-999kewdw	256	12	of	of	IN
cord-308835-999kewdw	256	13	the	the	DT
cord-308835-999kewdw	256	14	larger	large	JJR
cord-308835-999kewdw	256	15	RNA	RNA	NNP
cord-308835-999kewdw	256	16	species	specie	NNS
cord-308835-999kewdw	256	17	.	.	.
cord-308835-999kewdw	257	1	The	the	DT
cord-308835-999kewdw	257	2	fingerprint	fingerprint	NN
cord-308835-999kewdw	257	3	of	of	IN
cord-308835-999kewdw	257	4	RNA	RNA	NNP
cord-308835-999kewdw	257	5	species	species	NN
cord-308835-999kewdw	257	6	5	5	CD
cord-308835-999kewdw	257	7	also	also	RB
cord-308835-999kewdw	257	8	contains	contain	VBZ
cord-308835-999kewdw	257	9	a	a	DT
cord-308835-999kewdw	257	10	spot	spot	NN
cord-308835-999kewdw	257	11	which	which	WDT
cord-308835-999kewdw	257	12	is	be	VBZ
cord-308835-999kewdw	257	13	not	not	RB
cord-308835-999kewdw	257	14	present	present	JJ
cord-308835-999kewdw	257	15	in	in	IN
cord-308835-999kewdw	257	16	any	any	DT
cord-308835-999kewdw	257	17	other	other	JJ
cord-308835-999kewdw	257	18	RNA	RNA	NNP
cord-308835-999kewdw	257	19	species	specie	NNS
cord-308835-999kewdw	257	20	(	(	-LRB-
cord-308835-999kewdw	257	21	arrow	arrow	NN
cord-308835-999kewdw	257	22	in	in	IN
cord-308835-999kewdw	257	23	Fig	Fig	NNP
cord-308835-999kewdw	257	24	.	.	NNP
cord-308835-999kewdw	257	25	7	7	CD
cord-308835-999kewdw	257	26	,	,	,
cord-308835-999kewdw	257	27	panel	panel	NN
cord-308835-999kewdw	257	28	5	5	CD
cord-308835-999kewdw	257	29	)	)	-RRB-
cord-308835-999kewdw	257	30	.	.	.
cord-308835-999kewdw	258	1	The	the	DT
cord-308835-999kewdw	258	2	remaining	remain	VBG
cord-308835-999kewdw	258	3	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	258	4	in	in	IN
cord-308835-999kewdw	258	5	RNA	RNA	NNP
cord-308835-999kewdw	258	6	species	species	NN
cord-308835-999kewdw	258	7	5	5	CD
cord-308835-999kewdw	258	8	are	be	VBP
cord-308835-999kewdw	258	9	contained	contain	VBN
cord-308835-999kewdw	258	10	in	in	IN
cord-308835-999kewdw	258	11	the	the	DT
cord-308835-999kewdw	258	12	fingerprints	fingerprint	NNS
cord-308835-999kewdw	258	13	of	of	IN
cord-308835-999kewdw	258	14	the	the	DT
cord-308835-999kewdw	258	15	larger	large	JJR
cord-308835-999kewdw	258	16	RNA	RNA	NNP
cord-308835-999kewdw	258	17	species	specie	NNS
cord-308835-999kewdw	258	18	.	.	.
cord-308835-999kewdw	259	1	The	the	DT
cord-308835-999kewdw	259	2	fingerprint	fingerprint	NN
cord-308835-999kewdw	259	3	of	of	IN
cord-308835-999kewdw	259	4	RNA	RNA	NNP
cord-308835-999kewdw	259	5	species	species	NN
cord-308835-999kewdw	259	6	6	6	CD
cord-308835-999kewdw	259	7	is	be	VBZ
cord-308835-999kewdw	259	8	a	a	DT
cord-308835-999kewdw	259	9	subset	subset	NN
cord-308835-999kewdw	259	10	of	of	IN
cord-308835-999kewdw	259	11	that	that	DT
cord-308835-999kewdw	259	12	of	of	IN
cord-308835-999kewdw	259	13	RNA	RNA	NNP
cord-308835-999kewdw	259	14	species	specie	NNS
cord-308835-999kewdw	259	15	5	5	CD
cord-308835-999kewdw	259	16	.	.	.
cord-308835-999kewdw	260	1	Similarly	similarly	RB
cord-308835-999kewdw	260	2	,	,	,
cord-308835-999kewdw	260	3	all	all	PDT
cord-308835-999kewdw	260	4	the	the	DT
cord-308835-999kewdw	260	5	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	260	6	of	of	IN
cord-308835-999kewdw	260	7	RNA	RNA	NNP
cord-308835-999kewdw	260	8	species	species	NN
cord-308835-999kewdw	260	9	7	7	CD
cord-308835-999kewdw	260	10	are	be	VBP
cord-308835-999kewdw	260	11	contained	contain	VBN
cord-308835-999kewdw	260	12	in	in	IN
cord-308835-999kewdw	260	13	RNA	RNA	NNP
cord-308835-999kewdw	260	14	species	species	NNP
cord-308835-999kewdw	260	15	6	6	CD
cord-308835-999kewdw	260	16	.	.	.
cord-308835-999kewdw	261	1	However	however	RB
cord-308835-999kewdw	261	2	,	,	,
cord-308835-999kewdw	261	3	one	one	CD
cord-308835-999kewdw	261	4	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	261	5	(	(	-LRB-
cord-308835-999kewdw	261	6	closed	closed	JJ
cord-308835-999kewdw	261	7	triangle	triangle	NN
cord-308835-999kewdw	261	8	,	,	,
cord-308835-999kewdw	261	9	panels	panel	NNS
cord-308835-999kewdw	261	10	6	6	CD
cord-308835-999kewdw	261	11	and	and	CC
cord-308835-999kewdw	261	12	7	7	CD
cord-308835-999kewdw	261	13	)	)	-RRB-
cord-308835-999kewdw	261	14	is	be	VBZ
cord-308835-999kewdw	261	15	overrepresented	overrepresente	VBN
cord-308835-999kewdw	261	16	in	in	IN
cord-308835-999kewdw	261	17	RNAs	rna	NNS
cord-308835-999kewdw	261	18	6	6	CD
cord-308835-999kewdw	261	19	and	and	CC
cord-308835-999kewdw	261	20	7	7	CD
cord-308835-999kewdw	261	21	when	when	WRB
cord-308835-999kewdw	261	22	compared	compare	VBN
cord-308835-999kewdw	261	23	to	to	IN
cord-308835-999kewdw	261	24	virion	virion	NN
cord-308835-999kewdw	261	25	RNA	RNA	NNP
cord-308835-999kewdw	261	26	and	and	CC
cord-308835-999kewdw	261	27	RNAs	RNAs	NNP
cord-308835-999kewdw	261	28	2	2	CD
cord-308835-999kewdw	261	29	,	,	,
cord-308835-999kewdw	261	30	3	3	CD
cord-308835-999kewdw	261	31	,	,	,
cord-308835-999kewdw	261	32	4	4	CD
cord-308835-999kewdw	261	33	,	,	,
cord-308835-999kewdw	261	34	and	and	CC
cord-308835-999kewdw	261	35	5	5	CD
cord-308835-999kewdw	261	36	.	.	.
cord-308835-999kewdw	262	1	This	this	DT
cord-308835-999kewdw	262	2	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	262	3	has	have	VBZ
cord-308835-999kewdw	262	4	the	the	DT
cord-308835-999kewdw	262	5	same	same	JJ
cord-308835-999kewdw	262	6	Cytoplasmic	Cytoplasmic	NNP
cord-308835-999kewdw	262	7	extracts	extract	NNS
cord-308835-999kewdw	262	8	were	be	VBD
cord-308835-999kewdw	262	9	prepared	prepare	VBN
cord-308835-999kewdw	262	10	and	and	CC
cord-308835-999kewdw	262	11	the	the	DT
cord-308835-999kewdw	262	12	nucleic	nucleic	JJ
cord-308835-999kewdw	262	13	acids	acid	NNS
cord-308835-999kewdw	262	14	extracted	extract	VBN
cord-308835-999kewdw	262	15	and	and	CC
cord-308835-999kewdw	262	16	precipitated	precipitate	VBN
cord-308835-999kewdw	262	17	with	with	IN
cord-308835-999kewdw	262	18	ethanol	ethanol	NN
cord-308835-999kewdw	262	19	.	.	.
cord-308835-999kewdw	263	1	The	the	DT
cord-308835-999kewdw	263	2	nucleic	nucleic	JJ
cord-308835-999kewdw	263	3	acids	acid	NNS
cord-308835-999kewdw	263	4	were	be	VBD
cord-308835-999kewdw	263	5	dissolved	dissolve	VBN
cord-308835-999kewdw	263	6	in	in	IN
cord-308835-999kewdw	263	7	a	a	DT
cord-308835-999kewdw	263	8	buffer	buffer	NN
cord-308835-999kewdw	263	9	containing	contain	VBG
cord-308835-999kewdw	263	10	1	1	CD
cord-308835-999kewdw	263	11	mM	mm	CD
cord-308835-999kewdw	263	12	Tris	Tris	NNP
cord-308835-999kewdw	263	13	,	,	,
cord-308835-999kewdw	263	14	360	360	CD
cord-308835-999kewdw	263	15	mM	mm	CD
cord-308835-999kewdw	263	16	NaCl	nacl	NN
cord-308835-999kewdw	263	17	,	,	,
cord-308835-999kewdw	263	18	l	l	NN
cord-308835-999kewdw	263	19	mM	mM	NNP
cord-308835-999kewdw	263	20	EDTA	EDTA	NNP
cord-308835-999kewdw	263	21	,	,	,
cord-308835-999kewdw	263	22	0.5	0.5	CD
cord-308835-999kewdw	263	23	%	%	NN
cord-308835-999kewdw	263	24	SDS	SDS	NNP
cord-308835-999kewdw	263	25	,	,	,
cord-308835-999kewdw	263	26	pH	pH	NNP
cord-308835-999kewdw	263	27	7.4	7.4	CD
cord-308835-999kewdw	263	28	,	,	,
cord-308835-999kewdw	263	29	chromatographed	chromatographe	VBN
cord-308835-999kewdw	263	30	over	over	IN
cord-308835-999kewdw	263	31	poly(U	poly(u	$
cord-308835-999kewdw	263	32	)	)	-RRB-
cord-308835-999kewdw	264	1	Sepharose	Sepharose	NNP
cord-308835-999kewdw	264	2	as	as	IN
cord-308835-999kewdw	264	3	described	describe	VBN
cord-308835-999kewdw	264	4	by	by	IN
cord-308835-999kewdw	264	5	Wilt	Wilt	NNP
cord-308835-999kewdw	264	6	(	(	-LRB-
cord-308835-999kewdw	264	7	1977	1977	CD
cord-308835-999kewdw	264	8	)	)	-RRB-
cord-308835-999kewdw	264	9	,	,	,
cord-308835-999kewdw	264	10	and	and	CC
cord-308835-999kewdw	264	11	fractionated	fractionate	VBN
cord-308835-999kewdw	264	12	into	into	IN
cord-308835-999kewdw	264	13	two	two	CD
cord-308835-999kewdw	264	14	pools	pool	NNS
cord-308835-999kewdw	264	15	.	.	.
cord-308835-999kewdw	265	1	Pool	pool	NN
cord-308835-999kewdw	265	2	1	1	CD
cord-308835-999kewdw	265	3	was	be	VBD
cord-308835-999kewdw	265	4	not	not	RB
cord-308835-999kewdw	265	5	retained	retain	VBN
cord-308835-999kewdw	265	6	by	by	IN
cord-308835-999kewdw	265	7	the	the	DT
cord-308835-999kewdw	265	8	column	column	NN
cord-308835-999kewdw	265	9	;	;	:
cord-308835-999kewdw	265	10	pool	pool	NN
cord-308835-999kewdw	265	11	2	2	CD
cord-308835-999kewdw	265	12	contains	contain	VBZ
cord-308835-999kewdw	265	13	the	the	DT
cord-308835-999kewdw	265	14	RNA	RNA	NNP
cord-308835-999kewdw	265	15	which	which	WDT
cord-308835-999kewdw	265	16	bound	bind	VBN
cord-308835-999kewdw	265	17	to	to	IN
cord-308835-999kewdw	265	18	the	the	DT
cord-308835-999kewdw	265	19	poly(U	poly(U	JJS
cord-308835-999kewdw	265	20	)	)	-RRB-
cord-308835-999kewdw	265	21	Sepharose	Sepharose	NNP
cord-308835-999kewdw	265	22	.	.	.
cord-308835-999kewdw	266	1	Both	both	DT
cord-308835-999kewdw	266	2	pools	pool	NNS
cord-308835-999kewdw	266	3	were	be	VBD
cord-308835-999kewdw	266	4	precipitated	precipitate	VBN
cord-308835-999kewdw	266	5	with	with	IN
cord-308835-999kewdw	266	6	ethanol	ethanol	NN
cord-308835-999kewdw	266	7	after	after	IN
cord-308835-999kewdw	266	8	the	the	DT
cord-308835-999kewdw	266	9	addition	addition	NN
cord-308835-999kewdw	266	10	of	of	IN
cord-308835-999kewdw	266	11	carrier	carrier	NN
cord-308835-999kewdw	266	12	tRNA	tRNA	NNP
cord-308835-999kewdw	266	13	,	,	,
cord-308835-999kewdw	266	14	collected	collect	VBN
cord-308835-999kewdw	266	15	by	by	IN
cord-308835-999kewdw	266	16	centrifugation	centrifugation	NN
cord-308835-999kewdw	266	17	,	,	,
cord-308835-999kewdw	266	18	glyoxalated	glyoxalated	NNP
cord-308835-999kewdw	266	19	,	,	,
cord-308835-999kewdw	266	20	and	and	CC
cord-308835-999kewdw	266	21	analyzed	analyze	VBN
cord-308835-999kewdw	266	22	by	by	IN
cord-308835-999kewdw	266	23	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	266	24	on	on	IN
cord-308835-999kewdw	266	25	a	a	DT
cord-308835-999kewdw	266	26	0.8	0.8	CD
cord-308835-999kewdw	266	27	%	%	NN
cord-308835-999kewdw	266	28	agarose	agarose	NN
cord-308835-999kewdw	266	29	gel	gel	NN
cord-308835-999kewdw	266	30	.	.	.
cord-308835-999kewdw	267	1	The	the	DT
cord-308835-999kewdw	267	2	positions	position	NNS
cord-308835-999kewdw	267	3	of	of	IN
cord-308835-999kewdw	267	4	rRNAs	rrna	NNS
cord-308835-999kewdw	267	5	are	be	VBP
cord-308835-999kewdw	267	6	marked	mark	VBN
cord-308835-999kewdw	267	7	by	by	IN
cord-308835-999kewdw	267	8	wire	wire	NN
cord-308835-999kewdw	267	9	clips	clip	NNS
cord-308835-999kewdw	267	10	.	.	.
cord-308835-999kewdw	268	1	electrophoretic	electrophoretic	JJ
cord-308835-999kewdw	268	2	mobility	mobility	NN
cord-308835-999kewdw	268	3	as	as	IN
cord-308835-999kewdw	268	4	the	the	DT
cord-308835-999kewdw	268	5	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	268	6	which	which	WDT
cord-308835-999kewdw	268	7	is	be	VBZ
cord-308835-999kewdw	268	8	overrepresented	overrepresente	VBN
cord-308835-999kewdw	268	9	in	in	IN
cord-308835-999kewdw	268	10	RNA1	RNA1	NNP
cord-308835-999kewdw	268	11	and	and	CC
cord-308835-999kewdw	268	12	presumably	presumably	RB
cord-308835-999kewdw	268	13	is	be	VBZ
cord-308835-999kewdw	268	14	identical	identical	JJ
cord-308835-999kewdw	268	15	with	with	IN
cord-308835-999kewdw	268	16	it	-PRON-	PRP
cord-308835-999kewdw	268	17	.	.	.
cord-308835-999kewdw	269	1	An	an	DT
cord-308835-999kewdw	269	2	analysis	analysis	NN
cord-308835-999kewdw	269	3	of	of	IN
cord-308835-999kewdw	269	4	the	the	DT
cord-308835-999kewdw	269	5	data	datum	NNS
cord-308835-999kewdw	269	6	obtained	obtain	VBN
cord-308835-999kewdw	269	7	with	with	IN
cord-308835-999kewdw	269	8	JHMV	JHMV	NNP
cord-308835-999kewdw	269	9	(	(	-LRB-
cord-308835-999kewdw	269	10	Fig	Fig	NNP
cord-308835-999kewdw	269	11	.	.	.
cord-308835-999kewdw	269	12	8	8	CD
cord-308835-999kewdw	269	13	)	)	-RRB-
cord-308835-999kewdw	269	14	reveals	reveal	VBZ
cord-308835-999kewdw	269	15	similar	similar	JJ
cord-308835-999kewdw	269	16	results	result	NNS
cord-308835-999kewdw	269	17	.	.	.
cord-308835-999kewdw	270	1	RNA1	RNA1	NNP
cord-308835-999kewdw	270	2	is	be	VBZ
cord-308835-999kewdw	270	3	essentially	essentially	RB
cord-308835-999kewdw	270	4	identical	identical	JJ
cord-308835-999kewdw	270	5	to	to	IN
cord-308835-999kewdw	270	6	genomic	genomic	JJ
cord-308835-999kewdw	270	7	RNA	RNA	NNP
cord-308835-999kewdw	270	8	isolated	isolate	VBN
cord-308835-999kewdw	270	9	from	from	IN
cord-308835-999kewdw	270	10	purified	purify	VBN
cord-308835-999kewdw	270	11	virions	virion	NNS
cord-308835-999kewdw	270	12	with	with	IN
cord-308835-999kewdw	270	13	the	the	DT
cord-308835-999kewdw	270	14	exception	exception	NN
cord-308835-999kewdw	270	15	of	of	IN
cord-308835-999kewdw	270	16	an	an	DT
cord-308835-999kewdw	270	17	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	270	18	which	which	WDT
cord-308835-999kewdw	270	19	is	be	VBZ
cord-308835-999kewdw	270	20	underrepresented	underrepresente	VBN
cord-308835-999kewdw	270	21	in	in	IN
cord-308835-999kewdw	270	22	RNA1	RNA1	NNP
cord-308835-999kewdw	270	23	(	(	-LRB-
cord-308835-999kewdw	270	24	open	open	JJ
cord-308835-999kewdw	270	25	triangle	triangle	NN
cord-308835-999kewdw	270	26	)	)	-RRB-
cord-308835-999kewdw	270	27	.	.	.
cord-308835-999kewdw	271	1	The	the	DT
cord-308835-999kewdw	271	2	six	six	CD
cord-308835-999kewdw	271	3	subgenomic	subgenomic	JJ
cord-308835-999kewdw	271	4	JHMV	JHMV	NNP
cord-308835-999kewdw	271	5	-	-	HYPH
cord-308835-999kewdw	271	6	specific	specific	JJ
cord-308835-999kewdw	271	7	RNAs	rna	NNS
cord-308835-999kewdw	271	8	form	form	VBP
cord-308835-999kewdw	271	9	a	a	DT
cord-308835-999kewdw	271	10	nested	nest	VBN
cord-308835-999kewdw	271	11	set	set	NN
cord-308835-999kewdw	271	12	as	as	IN
cord-308835-999kewdw	271	13	described	describe	VBN
cord-308835-999kewdw	271	14	above	above	RB
cord-308835-999kewdw	271	15	for	for	IN
cord-308835-999kewdw	271	16	the	the	DT
cord-308835-999kewdw	271	17	A59V	A59V	NNP
cord-308835-999kewdw	271	18	specific	specific	JJ
cord-308835-999kewdw	271	19	RNA	RNA	NNP
cord-308835-999kewdw	271	20	species	specie	NNS
cord-308835-999kewdw	271	21	,	,	,
cord-308835-999kewdw	271	22	however	however	RB
cord-308835-999kewdw	271	23	,	,	,
cord-308835-999kewdw	271	24	as	as	IN
cord-308835-999kewdw	271	25	for	for	IN
cord-308835-999kewdw	271	26	A59V	a59v	NN
cord-308835-999kewdw	271	27	,	,	,
cord-308835-999kewdw	271	28	one	one	CD
cord-308835-999kewdw	271	29	oligonucleotide	oligonucleotide	NN
cord-308835-999kewdw	271	30	(	(	-LRB-
cord-308835-999kewdw	271	31	closed	closed	JJ
cord-308835-999kewdw	271	32	triangle	triangle	NN
cord-308835-999kewdw	271	33	)	)	-RRB-
cord-308835-999kewdw	271	34	is	be	VBZ
cord-308835-999kewdw	271	35	markedly	markedly	RB
cord-308835-999kewdw	271	36	overrepresented	overrepresente	VBN
cord-308835-999kewdw	271	37	in	in	IN
cord-308835-999kewdw	271	38	RNAs	rna	NNS
cord-308835-999kewdw	271	39	6	6	CD
cord-308835-999kewdw	271	40	and	and	CC
cord-308835-999kewdw	271	41	7	7	CD
cord-308835-999kewdw	271	42	when	when	WRB
cord-308835-999kewdw	271	43	compared	compare	VBN
cord-308835-999kewdw	271	44	to	to	IN
cord-308835-999kewdw	271	45	RNAs	rna	NNS
cord-308835-999kewdw	271	46	2	2	CD
cord-308835-999kewdw	271	47	-	-	SYM
cord-308835-999kewdw	271	48	5	5	CD
cord-308835-999kewdw	271	49	.	.	.
cord-308835-999kewdw	272	1	The	the	DT
cord-308835-999kewdw	272	2	fingerprint	fingerprint	NN
cord-308835-999kewdw	272	3	of	of	IN
cord-308835-999kewdw	272	4	RNA	RNA	NNP
cord-308835-999kewdw	272	5	species	specie	NNS
cord-308835-999kewdw	272	6	4	4	CD
cord-308835-999kewdw	272	7	contains	contain	VBZ
cord-308835-999kewdw	272	8	a	a	DT
cord-308835-999kewdw	272	9	spot	spot	NN
cord-308835-999kewdw	272	10	which	which	WDT
cord-308835-999kewdw	272	11	is	be	VBZ
cord-308835-999kewdw	272	12	not	not	RB
cord-308835-999kewdw	272	13	present	present	JJ
cord-308835-999kewdw	272	14	in	in	IN
cord-308835-999kewdw	272	15	any	any	DT
cord-308835-999kewdw	272	16	other	other	JJ
cord-308835-999kewdw	272	17	JHMV	JHMV	NNP
cord-308835-999kewdw	272	18	-	-	HYPH
cord-308835-999kewdw	272	19	specific	specific	JJ
cord-308835-999kewdw	272	20	RNA	RNA	NNP
cord-308835-999kewdw	272	21	(	(	-LRB-
cord-308835-999kewdw	272	22	arrow	arrow	NN
cord-308835-999kewdw	272	23	in	in	IN
cord-308835-999kewdw	272	24	Fig	Fig	NNP
cord-308835-999kewdw	272	25	.	.	.
cord-308835-999kewdw	273	1	8	8	CD
cord-308835-999kewdw	273	2	,	,	,
cord-308835-999kewdw	273	3	panel	panel	NN
cord-308835-999kewdw	273	4	4	4	CD
cord-308835-999kewdw	273	5	)	)	-RRB-
cord-308835-999kewdw	273	6	.	.	.
cord-308835-999kewdw	274	1	The	the	DT
cord-308835-999kewdw	274	2	reason	reason	NN
cord-308835-999kewdw	274	3	for	for	IN
cord-308835-999kewdw	274	4	the	the	DT
cord-308835-999kewdw	274	5	overrepresentation	overrepresentation	NN
cord-308835-999kewdw	274	6	of	of	IN
cord-308835-999kewdw	274	7	some	some	DT
cord-308835-999kewdw	274	8	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	274	9	and	and	CC
cord-308835-999kewdw	274	10	the	the	DT
cord-308835-999kewdw	274	11	underrepresentation	underrepresentation	NN
cord-308835-999kewdw	274	12	of	of	IN
cord-308835-999kewdw	274	13	others	other	NNS
cord-308835-999kewdw	274	14	in	in	IN
cord-308835-999kewdw	274	15	these	these	DT
cord-308835-999kewdw	274	16	fingerprints	fingerprint	NNS
cord-308835-999kewdw	274	17	is	be	VBZ
cord-308835-999kewdw	274	18	unclear	unclear	JJ
cord-308835-999kewdw	274	19	at	at	IN
cord-308835-999kewdw	274	20	present	present	JJ
cord-308835-999kewdw	274	21	.	.	.
cord-308835-999kewdw	275	1	The	the	DT
cord-308835-999kewdw	275	2	spots	spot	NNS
cord-308835-999kewdw	275	3	which	which	WDT
cord-308835-999kewdw	275	4	are	be	VBP
cord-308835-999kewdw	275	5	underrepresented	underrepresente	VBN
cord-308835-999kewdw	275	6	in	in	IN
cord-308835-999kewdw	275	7	RNA1	RNA1	NNP
cord-308835-999kewdw	275	8	when	when	WRB
cord-308835-999kewdw	275	9	compared	compare	VBN
cord-308835-999kewdw	275	10	to	to	IN
cord-308835-999kewdw	275	11	virion	virion	NN
cord-308835-999kewdw	275	12	RNA	RNA	NNP
cord-308835-999kewdw	275	13	disappear	disappear	VBP
cord-308835-999kewdw	275	14	in	in	IN
cord-308835-999kewdw	275	15	RNA	RNA	NNP
cord-308835-999kewdw	275	16	2	2	CD
cord-308835-999kewdw	275	17	.	.	.
cord-308835-999kewdw	276	1	These	these	DT
cord-308835-999kewdw	276	2	may	may	MD
cord-308835-999kewdw	276	3	represent	represent	VB
cord-308835-999kewdw	276	4	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	276	5	from	from	IN
cord-308835-999kewdw	276	6	the	the	DT
cord-308835-999kewdw	276	7	5	5	CD
cord-308835-999kewdw	276	8	'	'	NN
cord-308835-999kewdw	276	9	end	end	NN
cord-308835-999kewdw	276	10	of	of	IN
cord-308835-999kewdw	276	11	the	the	DT
cord-308835-999kewdw	276	12	genome	genome	NN
cord-308835-999kewdw	276	13	which	which	WDT
cord-308835-999kewdw	276	14	are	be	VBP
cord-308835-999kewdw	276	15	selectively	selectively	RB
cord-308835-999kewdw	276	16	lost	lose	VBN
cord-308835-999kewdw	276	17	during	during	IN
cord-308835-999kewdw	276	18	the	the	DT
cord-308835-999kewdw	276	19	poly(U	poly(u	NN
cord-308835-999kewdw	276	20	)	)	-RRB-
cord-308835-999kewdw	277	1	Sepharose	sepharose	NN
cord-308835-999kewdw	277	2	selection	selection	NN
cord-308835-999kewdw	277	3	in	in	IN
cord-308835-999kewdw	277	4	the	the	DT
cord-308835-999kewdw	277	5	purification	purification	NN
cord-308835-999kewdw	277	6	of	of	IN
cord-308835-999kewdw	277	7	RNAl	RNAl	NNS
cord-308835-999kewdw	277	8	.	.	.
cord-308835-999kewdw	278	1	A	a	DT
cord-308835-999kewdw	278	2	more	more	RBR
cord-308835-999kewdw	278	3	extensive	extensive	JJ
cord-308835-999kewdw	278	4	analysis	analysis	NN
cord-308835-999kewdw	278	5	of	of	IN
cord-308835-999kewdw	278	6	the	the	DT
cord-308835-999kewdw	278	7	structure	structure	NN
cord-308835-999kewdw	278	8	of	of	IN
cord-308835-999kewdw	278	9	MHV	MHV	NNP
cord-308835-999kewdw	278	10	RNA	RNA	NNP
cord-308835-999kewdw	278	11	should	should	MD
cord-308835-999kewdw	278	12	clarify	clarify	VB
cord-308835-999kewdw	278	13	this	this	DT
cord-308835-999kewdw	278	14	point	point	NN
cord-308835-999kewdw	278	15	.	.	.
cord-308835-999kewdw	279	1	A	a	DT
cord-308835-999kewdw	279	2	comparison	comparison	NN
cord-308835-999kewdw	279	3	of	of	IN
cord-308835-999kewdw	279	4	the	the	DT
cord-308835-999kewdw	279	5	fingerprints	fingerprint	NNS
cord-308835-999kewdw	279	6	of	of	IN
cord-308835-999kewdw	279	7	A59V	a59v	NN
cord-308835-999kewdw	279	8	and	and	CC
cord-308835-999kewdw	279	9	JHMV	JHMV	NNP
cord-308835-999kewdw	279	10	virion	virion	NN
cord-308835-999kewdw	279	11	RNAs	rna	NNS
cord-308835-999kewdw	279	12	shows	show	VBZ
cord-308835-999kewdw	279	13	only	only	RB
cord-308835-999kewdw	279	14	10	10	CD
cord-308835-999kewdw	279	15	to	to	TO
cord-308835-999kewdw	279	16	20	20	CD
cord-308835-999kewdw	279	17	%	%	NN
cord-308835-999kewdw	279	18	sequence	sequence	NN
cord-308835-999kewdw	279	19	homology	homology	NN
cord-308835-999kewdw	279	20	between	between	IN
cord-308835-999kewdw	279	21	these	these	DT
cord-308835-999kewdw	279	22	two	two	CD
cord-308835-999kewdw	279	23	viruses	virus	NNS
cord-308835-999kewdw	279	24	.	.	.
cord-308835-999kewdw	280	1	This	this	DT
cord-308835-999kewdw	280	2	is	be	VBZ
cord-308835-999kewdw	280	3	in	in	IN
cord-308835-999kewdw	280	4	contrast	contrast	NN
cord-308835-999kewdw	280	5	to	to	IN
cord-308835-999kewdw	280	6	data	datum	NNS
cord-308835-999kewdw	280	7	obtained	obtain	VBN
cord-308835-999kewdw	280	8	by	by	IN
cord-308835-999kewdw	280	9	hybridization	hybridization	NN
cord-308835-999kewdw	280	10	analysis	analysis	NN
cord-308835-999kewdw	280	11	FIG	fig	NN
cord-308835-999kewdw	280	12	.	.	.
cord-308835-999kewdw	281	1	7	7	CD
cord-308835-999kewdw	281	2	.	.	.
cord-308835-999kewdw	282	1	Oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	282	2	fingerprints	fingerprint	NNS
cord-308835-999kewdw	282	3	of	of	IN
cord-308835-999kewdw	282	4	A596-specific	A596-specific	NNP
cord-308835-999kewdw	282	5	RNA	RNA	NNP
cord-308835-999kewdw	282	6	.	.	.
cord-308835-999kewdw	283	1	A59V	A59V	NNP
cord-308835-999kewdw	283	2	virion	virion	NN
cord-308835-999kewdw	283	3	(	(	-LRB-
cord-308835-999kewdw	283	4	panel	panel	NN
cord-308835-999kewdw	283	5	V	v	NN
cord-308835-999kewdw	283	6	)	)	-RRB-
cord-308835-999kewdw	283	7	and	and	CC
cord-308835-999kewdw	283	8	intracellular	intracellular	JJ
cord-308835-999kewdw	283	9	RNAs	rna	NNS
cord-308835-999kewdw	283	10	(	(	-LRB-
cord-308835-999kewdw	283	11	panels	panel	NNS
cord-308835-999kewdw	283	12	l-7	l-7	VBP
cord-308835-999kewdw	283	13	)	)	-RRB-
cord-308835-999kewdw	283	14	were	be	VBD
cord-308835-999kewdw	283	15	purified	purify	VBN
cord-308835-999kewdw	283	16	as	as	IN
cord-308835-999kewdw	283	17	described	describe	VBN
cord-308835-999kewdw	283	18	under	under	IN
cord-308835-999kewdw	283	19	Materials	material	NNS
cord-308835-999kewdw	283	20	and	and	CC
cord-308835-999kewdw	283	21	Methods	method	NNS
cord-308835-999kewdw	283	22	.	.	.
cord-308835-999kewdw	284	1	The	the	DT
cord-308835-999kewdw	284	2	purified	purify	VBN
cord-308835-999kewdw	284	3	RNAs	rna	NNS
cord-308835-999kewdw	284	4	were	be	VBD
cord-308835-999kewdw	284	5	digested	digest	VBN
cord-308835-999kewdw	284	6	with	with	IN
cord-308835-999kewdw	284	7	RNAse	RNAse	NNP
cord-308835-999kewdw	284	8	T1	T1	NNP
cord-308835-999kewdw	284	9	and	and	CC
cord-308835-999kewdw	284	10	the	the	DT
cord-308835-999kewdw	284	11	resulting	result	VBG
cord-308835-999kewdw	284	12	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	284	13	separated	separate	VBN
cord-308835-999kewdw	284	14	by	by	IN
cord-308835-999kewdw	284	15	two	two	CD
cord-308835-999kewdw	284	16	-	-	HYPH
cord-308835-999kewdw	284	17	dimensional	dimensional	JJ
cord-308835-999kewdw	284	18	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	284	19	and	and	CC
cord-308835-999kewdw	284	20	autoradiographs	autoradiographs	NNP
cord-308835-999kewdw	284	21	prepared	prepare	VBD
cord-308835-999kewdw	284	22	as	as	IN
cord-308835-999kewdw	284	23	described	describe	VBN
cord-308835-999kewdw	284	24	under	under	IN
cord-308835-999kewdw	284	25	Materials	material	NNS
cord-308835-999kewdw	284	26	and	and	CC
cord-308835-999kewdw	284	27	Methods	method	NNS
cord-308835-999kewdw	284	28	.	.	.
cord-308835-999kewdw	285	1	Only	only	RB
cord-308835-999kewdw	285	2	the	the	DT
cord-308835-999kewdw	285	3	portion	portion	NN
cord-308835-999kewdw	285	4	of	of	IN
cord-308835-999kewdw	285	5	the	the	DT
cord-308835-999kewdw	285	6	autoradiographs	autoradiographs	NN
cord-308835-999kewdw	285	7	which	which	WDT
cord-308835-999kewdw	285	8	contains	contain	VBZ
cord-308835-999kewdw	285	9	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	285	10	migrating	migrate	VBG
cord-308835-999kewdw	285	11	more	more	RBR
cord-308835-999kewdw	285	12	slowly	slowly	RB
cord-308835-999kewdw	285	13	than	than	IN
cord-308835-999kewdw	285	14	the	the	DT
cord-308835-999kewdw	285	15	xylene	xylene	NN
cord-308835-999kewdw	285	16	cyan01	cyan01	NNS
cord-308835-999kewdw	285	17	dye	dye	NN
cord-308835-999kewdw	285	18	marker	marker	NN
cord-308835-999kewdw	285	19	is	be	VBZ
cord-308835-999kewdw	285	20	shown	show	VBN
cord-308835-999kewdw	285	21	.	.	.
cord-308835-999kewdw	286	1	The	the	DT
cord-308835-999kewdw	286	2	positions	position	NNS
cord-308835-999kewdw	286	3	of	of	IN
cord-308835-999kewdw	286	4	the	the	DT
cord-308835-999kewdw	286	5	bromphenol	bromphenol	JJ
cord-308835-999kewdw	286	6	blue	blue	JJ
cord-308835-999kewdw	286	7	dye	dye	NN
cord-308835-999kewdw	286	8	markers	marker	NNS
cord-308835-999kewdw	286	9	are	be	VBP
cord-308835-999kewdw	286	10	idicated	idicate	VBN
cord-308835-999kewdw	286	11	by	by	IN
cord-308835-999kewdw	286	12	asterisks	asterisk	NNS
cord-308835-999kewdw	286	13	.	.	.
cord-308835-999kewdw	287	1	Unique	unique	JJ
cord-308835-999kewdw	287	2	spots	spot	NNS
cord-308835-999kewdw	287	3	are	be	VBP
cord-308835-999kewdw	287	4	indicated	indicate	VBN
cord-308835-999kewdw	287	5	by	by	IN
cord-308835-999kewdw	287	6	arrows	arrow	NNS
cord-308835-999kewdw	287	7	.	.	.
cord-308835-999kewdw	288	1	which	which	WDT
cord-308835-999kewdw	288	2	indicates	indicate	VBZ
cord-308835-999kewdw	288	3	75	75	CD
cord-308835-999kewdw	288	4	%	%	NN
cord-308835-999kewdw	288	5	homology	homology	NN
cord-308835-999kewdw	288	6	between	between	IN
cord-308835-999kewdw	288	7	the	the	DT
cord-308835-999kewdw	288	8	two	two	CD
cord-308835-999kewdw	288	9	genomes	genome	NNS
cord-308835-999kewdw	288	10	(	(	-LRB-
cord-308835-999kewdw	288	11	Weiss	Weiss	NNP
cord-308835-999kewdw	288	12	and	and	CC
cord-308835-999kewdw	288	13	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	288	14	,	,	,
cord-308835-999kewdw	288	15	in	in	IN
cord-308835-999kewdw	288	16	press	press	NN
cord-308835-999kewdw	288	17	)	)	-RRB-
cord-308835-999kewdw	288	18	and	and	CC
cord-308835-999kewdw	288	19	the	the	DT
cord-308835-999kewdw	288	20	serologic	serologic	NNP
cord-308835-999kewdw	288	21	cross	cross	NNP
cord-308835-999kewdw	288	22	reactions	reaction	NNS
cord-308835-999kewdw	288	23	obtained	obtain	VBN
cord-308835-999kewdw	288	24	with	with	IN
cord-308835-999kewdw	288	25	antisera	antisera	NN
cord-308835-999kewdw	288	26	to	to	IN
cord-308835-999kewdw	288	27	these	these	DT
cord-308835-999kewdw	288	28	viruses	virus	NNS
cord-308835-999kewdw	288	29	(	(	-LRB-
cord-308835-999kewdw	288	30	Bond	Bond	NNP
cord-308835-999kewdw	288	31	et	et	NNP
cord-308835-999kewdw	288	32	al	al	NNP
cord-308835-999kewdw	288	33	.	.	NNP
cord-308835-999kewdw	288	34	,	,	,
cord-308835-999kewdw	288	35	1979	1979	CD
cord-308835-999kewdw	288	36	)	)	-RRB-
cord-308835-999kewdw	288	37	.	.	.
cord-308835-999kewdw	289	1	This	this	DT
cord-308835-999kewdw	289	2	discrepancy	discrepancy	NN
cord-308835-999kewdw	289	3	is	be	VBZ
cord-308835-999kewdw	289	4	not	not	RB
cord-308835-999kewdw	289	5	surprising	surprising	JJ
cord-308835-999kewdw	289	6	since	since	IN
cord-308835-999kewdw	289	7	T1	t1	NN
cord-308835-999kewdw	289	8	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	289	9	detects	detect	VBZ
cord-308835-999kewdw	289	10	single	single	JJ
cord-308835-999kewdw	289	11	base	base	NN
cord-308835-999kewdw	289	12	changes	change	NNS
cord-308835-999kewdw	289	13	and	and	CC
cord-308835-999kewdw	289	14	will	will	MD
cord-308835-999kewdw	289	15	underestimate	underestimate	VB
cord-308835-999kewdw	289	16	the	the	DT
cord-308835-999kewdw	289	17	degree	degree	NN
cord-308835-999kewdw	289	18	of	of	IN
cord-308835-999kewdw	289	19	homology	homology	NN
cord-308835-999kewdw	289	20	.	.	.
cord-308835-999kewdw	290	1	The	the	DT
cord-308835-999kewdw	290	2	data	datum	NNS
cord-308835-999kewdw	290	3	presented	present	VBN
cord-308835-999kewdw	290	4	above	above	IN
cord-308835-999kewdw	290	5	indicate	indicate	VBP
cord-308835-999kewdw	290	6	that	that	IN
cord-308835-999kewdw	290	7	MHV	mhv	NN
cord-308835-999kewdw	290	8	-	-	HYPH
cord-308835-999kewdw	290	9	infected	infect	VBN
cord-308835-999kewdw	290	10	cells	cell	NNS
cord-308835-999kewdw	290	11	synthesize	synthesize	VBP
cord-308835-999kewdw	290	12	at	at	IN
cord-308835-999kewdw	290	13	least	least	RBS
cord-308835-999kewdw	290	14	seven	seven	CD
cord-308835-999kewdw	290	15	virus	virus	NN
cord-308835-999kewdw	290	16	-	-	HYPH
cord-308835-999kewdw	290	17	specific	specific	JJ
cord-308835-999kewdw	290	18	RNAs	rna	NNS
cord-308835-999kewdw	290	19	.	.	.
cord-308835-999kewdw	291	1	The	the	DT
cord-308835-999kewdw	291	2	largest	large	JJS
cord-308835-999kewdw	291	3	of	of	IN
cord-308835-999kewdw	291	4	these	these	DT
cord-308835-999kewdw	291	5	RNAs	rna	NNS
cord-308835-999kewdw	291	6	,	,	,
cord-308835-999kewdw	291	7	RNAl	RNAl	NNS
cord-308835-999kewdw	291	8	,	,	,
cord-308835-999kewdw	291	9	is	be	VBZ
cord-308835-999kewdw	291	10	nearly	nearly	RB
cord-308835-999kewdw	291	11	identical	identical	JJ
cord-308835-999kewdw	291	12	to	to	IN
cord-308835-999kewdw	291	13	virion	virion	NN
cord-308835-999kewdw	291	14	RNA	RNA	NNP
cord-308835-999kewdw	291	15	,	,	,
cord-308835-999kewdw	291	16	as	as	IN
cord-308835-999kewdw	291	17	judged	judge	VBN
cord-308835-999kewdw	291	18	by	by	IN
cord-308835-999kewdw	291	19	oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	291	20	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	291	21	.	.	.
cord-308835-999kewdw	292	1	The	the	DT
cord-308835-999kewdw	292	2	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	292	3	of	of	IN
cord-308835-999kewdw	292	4	the	the	DT
cord-308835-999kewdw	292	5	six	six	CD
cord-308835-999kewdw	292	6	subgenomic	subgenomic	NNP
cord-308835-999kewdw	292	7	RNAs	rna	NNS
cord-308835-999kewdw	292	8	,	,	,
cord-308835-999kewdw	292	9	RNAs	rna	NNS
cord-308835-999kewdw	292	10	2	2	CD
cord-308835-999kewdw	292	11	-	-	SYM
cord-308835-999kewdw	292	12	7	7	CD
cord-308835-999kewdw	292	13	,	,	,
cord-308835-999kewdw	292	14	are	be	VBP
cord-308835-999kewdw	292	15	contained	contain	VBN
cord-308835-999kewdw	292	16	in	in	IN
cord-308835-999kewdw	292	17	the	the	DT
cord-308835-999kewdw	292	18	virion	virion	NN
cord-308835-999kewdw	292	19	RNA	RNA	NNP
cord-308835-999kewdw	292	20	.	.	.
cord-308835-999kewdw	293	1	They	-PRON-	PRP
cord-308835-999kewdw	293	2	are	be	VBP
cord-308835-999kewdw	293	3	therefore	therefore	RB
cord-308835-999kewdw	293	4	of	of	IN
cord-308835-999kewdw	293	5	the	the	DT
cord-308835-999kewdw	293	6	same	same	JJ
cord-308835-999kewdw	293	7	polarity	polarity	NN
cord-308835-999kewdw	293	8	as	as	IN
cord-308835-999kewdw	293	9	the	the	DT
cord-308835-999kewdw	293	10	virion	virion	NN
cord-308835-999kewdw	293	11	RNA	RNA	NNP
cord-308835-999kewdw	293	12	.	.	.
cord-308835-999kewdw	294	1	Oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	294	2	fingerprints	fingerprint	NNS
cord-308835-999kewdw	294	3	of	of	IN
cord-308835-999kewdw	294	4	these	these	DT
cord-308835-999kewdw	294	5	FIG	fig	NN
cord-308835-999kewdw	294	6	.	.	.
cord-308835-999kewdw	295	1	8	8	CD
cord-308835-999kewdw	295	2	.	.	.
cord-308835-999kewdw	296	1	Oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	296	2	fingerprints	fingerprint	NNS
cord-308835-999kewdw	296	3	of	of	IN
cord-308835-999kewdw	296	4	JHMV	JHMV	NNP
cord-308835-999kewdw	296	5	-	-	HYPH
cord-308835-999kewdw	296	6	specific	specific	JJ
cord-308835-999kewdw	296	7	RNA	RNA	NNP
cord-308835-999kewdw	296	8	.	.	.
cord-308835-999kewdw	297	1	JHMV	JHMV	NNP
cord-308835-999kewdw	297	2	virion	virion	NN
cord-308835-999kewdw	297	3	(	(	-LRB-
cord-308835-999kewdw	297	4	panel	panel	NN
cord-308835-999kewdw	297	5	V	v	NN
cord-308835-999kewdw	297	6	)	)	-RRB-
cord-308835-999kewdw	297	7	and	and	CC
cord-308835-999kewdw	297	8	intracellular	intracellular	JJ
cord-308835-999kewdw	297	9	RNAs	rna	NNS
cord-308835-999kewdw	297	10	(	(	-LRB-
cord-308835-999kewdw	297	11	panels	panel	NNS
cord-308835-999kewdw	297	12	1	1	CD
cord-308835-999kewdw	297	13	-	-	SYM
cord-308835-999kewdw	297	14	7	7	CD
cord-308835-999kewdw	297	15	)	)	-RRB-
cord-308835-999kewdw	297	16	were	be	VBD
cord-308835-999kewdw	297	17	purified	purify	VBN
cord-308835-999kewdw	297	18	as	as	IN
cord-308835-999kewdw	297	19	described	describe	VBN
cord-308835-999kewdw	297	20	under	under	IN
cord-308835-999kewdw	297	21	Materials	material	NNS
cord-308835-999kewdw	297	22	and	and	CC
cord-308835-999kewdw	297	23	Methods	method	NNS
cord-308835-999kewdw	297	24	.	.	.
cord-308835-999kewdw	298	1	The	the	DT
cord-308835-999kewdw	298	2	purified	purify	VBN
cord-308835-999kewdw	298	3	RNAs	rna	NNS
cord-308835-999kewdw	298	4	were	be	VBD
cord-308835-999kewdw	298	5	digested	digest	VBN
cord-308835-999kewdw	298	6	with	with	IN
cord-308835-999kewdw	298	7	RNAse	RNAse	NNP
cord-308835-999kewdw	298	8	Ti	Ti	NNP
cord-308835-999kewdw	298	9	,	,	,
cord-308835-999kewdw	298	10	the	the	DT
cord-308835-999kewdw	298	11	oligonucleotides	oligonucleotide	NNS
cord-308835-999kewdw	298	12	separated	separate	VBN
cord-308835-999kewdw	298	13	by	by	IN
cord-308835-999kewdw	298	14	two	two	CD
cord-308835-999kewdw	298	15	-	-	HYPH
cord-308835-999kewdw	298	16	dimensional	dimensional	JJ
cord-308835-999kewdw	298	17	electrophoresis	electrophoresis	NN
cord-308835-999kewdw	298	18	,	,	,
cord-308835-999kewdw	298	19	and	and	CC
cord-308835-999kewdw	298	20	autoradiographs	autoradiographs	NNP
cord-308835-999kewdw	298	21	prepared	prepare	VBD
cord-308835-999kewdw	298	22	.	.	.
cord-308835-999kewdw	299	1	The	the	DT
cord-308835-999kewdw	299	2	positions	position	NNS
cord-308835-999kewdw	299	3	of	of	IN
cord-308835-999kewdw	299	4	the	the	DT
cord-308835-999kewdw	299	5	bromphenol	bromphenol	JJ
cord-308835-999kewdw	299	6	dye	dye	NN
cord-308835-999kewdw	299	7	marker	marker	NN
cord-308835-999kewdw	299	8	are	be	VBP
cord-308835-999kewdw	299	9	shown	show	VBN
cord-308835-999kewdw	299	10	by	by	IN
cord-308835-999kewdw	299	11	asterisks	asterisk	NNS
cord-308835-999kewdw	299	12	.	.	.
cord-308835-999kewdw	300	1	Unique	unique	JJ
cord-308835-999kewdw	300	2	spots	spot	NNS
cord-308835-999kewdw	300	3	are	be	VBP
cord-308835-999kewdw	300	4	indicated	indicate	VBN
cord-308835-999kewdw	300	5	by	by	IN
cord-308835-999kewdw	300	6	arrows	arrow	NNS
cord-308835-999kewdw	300	7	.	.	.
cord-308835-999kewdw	301	1	RNAs	rna	NNS
cord-308835-999kewdw	301	2	show	show	VBP
cord-308835-999kewdw	301	3	them	-PRON-	PRP
cord-308835-999kewdw	301	4	to	to	TO
cord-308835-999kewdw	301	5	make	make	VB
cord-308835-999kewdw	301	6	up	up	RP
cord-308835-999kewdw	301	7	a	a	DT
cord-308835-999kewdw	301	8	nested	nest	VBN
cord-308835-999kewdw	301	9	set	set	NN
cord-308835-999kewdw	301	10	in	in	IN
cord-308835-999kewdw	301	11	which	which	WDT
cord-308835-999kewdw	301	12	any	any	DT
cord-308835-999kewdw	301	13	RNA	RNA	NNP
cord-308835-999kewdw	301	14	contains	contain	VBZ
cord-308835-999kewdw	301	15	the	the	DT
cord-308835-999kewdw	301	16	sequences	sequence	NNS
cord-308835-999kewdw	301	17	present	present	JJ
cord-308835-999kewdw	301	18	in	in	IN
cord-308835-999kewdw	301	19	any	any	DT
cord-308835-999kewdw	301	20	other	other	JJ
cord-308835-999kewdw	301	21	smaller	small	JJR
cord-308835-999kewdw	301	22	RNA	RNA	NNP
cord-308835-999kewdw	301	23	plus	plus	CC
cord-308835-999kewdw	301	24	additional	additional	JJ
cord-308835-999kewdw	301	25	sequences	sequence	NNS
cord-308835-999kewdw	301	26	consistent	consistent	JJ
cord-308835-999kewdw	301	27	with	with	IN
cord-308835-999kewdw	301	28	its	-PRON-	PRP$
cord-308835-999kewdw	301	29	larger	large	JJR
cord-308835-999kewdw	301	30	size	size	NN
cord-308835-999kewdw	301	31	.	.	.
cord-308835-999kewdw	302	1	Similar	similar	JJ
cord-308835-999kewdw	302	2	data	datum	NNS
cord-308835-999kewdw	302	3	have	have	VBP
cord-308835-999kewdw	302	4	been	be	VBN
cord-308835-999kewdw	302	5	obtained	obtain	VBN
cord-308835-999kewdw	302	6	with	with	IN
cord-308835-999kewdw	302	7	another	another	DT
cord-308835-999kewdw	302	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	302	9	,	,	,
cord-308835-999kewdw	302	10	IBV	IBV	NNP
cord-308835-999kewdw	302	11	(	(	-LRB-
cord-308835-999kewdw	302	12	Stern	Stern	NNP
cord-308835-999kewdw	302	13	and	and	CC
cord-308835-999kewdw	302	14	Kennedy	Kennedy	NNP
cord-308835-999kewdw	302	15	,	,	,
cord-308835-999kewdw	302	16	1980a	1980a	CD
cord-308835-999kewdw	302	17	)	)	-RRB-
cord-308835-999kewdw	302	18	.	.	.
cord-308835-999kewdw	303	1	A	a	DT
cord-308835-999kewdw	303	2	"	"	``
cord-308835-999kewdw	303	3	Northern	northern	JJ
cord-308835-999kewdw	303	4	blot	blot	NN
cord-308835-999kewdw	303	5	"	"	''
cord-308835-999kewdw	303	6	analysis	analysis	NN
cord-308835-999kewdw	303	7	using	use	VBG
cord-308835-999kewdw	303	8	representative	representative	JJ
cord-308835-999kewdw	303	9	and	and	CC
cord-308835-999kewdw	303	10	short	short	JJ
cord-308835-999kewdw	303	11	3	3	CD
cord-308835-999kewdw	303	12	'	'	''
cord-308835-999kewdw	303	13	specific	specific	JJ
cord-308835-999kewdw	303	14	cDNA	cdna	NN
cord-308835-999kewdw	303	15	probes	probe	NNS
cord-308835-999kewdw	303	16	indicates	indicate	VBZ
cord-308835-999kewdw	303	17	that	that	IN
cord-308835-999kewdw	303	18	the	the	DT
cord-308835-999kewdw	303	19	six	six	CD
cord-308835-999kewdw	303	20	subgenomic	subgenomic	NNP
cord-308835-999kewdw	303	21	RNAs	RNAs	NNPS
cord-308835-999kewdw	303	22	share	share	VBP
cord-308835-999kewdw	303	23	sequences	sequence	NNS
cord-308835-999kewdw	303	24	at	at	IN
cord-308835-999kewdw	303	25	their	-PRON-	PRP$
cord-308835-999kewdw	303	26	3	3	CD
cord-308835-999kewdw	303	27	'	'	''
cord-308835-999kewdw	303	28	ends	end	NNS
cord-308835-999kewdw	303	29	(	(	-LRB-
cord-308835-999kewdw	303	30	Weiss	Weiss	NNP
cord-308835-999kewdw	303	31	and	and	CC
cord-308835-999kewdw	303	32	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	303	33	,	,	,
cord-308835-999kewdw	303	34	in	in	IN
cord-308835-999kewdw	303	35	press	press	NN
cord-308835-999kewdw	303	36	)	)	-RRB-
cord-308835-999kewdw	303	37	.	.	.
cord-308835-999kewdw	304	1	Furthermore	furthermore	RB
cord-308835-999kewdw	304	2	,	,	,
cord-308835-999kewdw	304	3	Stern	Stern	NNP
cord-308835-999kewdw	304	4	and	and	CC
cord-308835-999kewdw	304	5	Kennedy	Kennedy	NNP
cord-308835-999kewdw	304	6	(	(	-LRB-
cord-308835-999kewdw	304	7	1980b	1980b	CD
cord-308835-999kewdw	304	8	)	)	-RRB-
cord-308835-999kewdw	304	9	have	have	VBP
cord-308835-999kewdw	304	10	established	establish	VBN
cord-308835-999kewdw	304	11	a	a	DT
cord-308835-999kewdw	304	12	5	5	CD
cord-308835-999kewdw	304	13	'	'	''
cord-308835-999kewdw	304	14	to	to	IN
cord-308835-999kewdw	304	15	3	3	CD
cord-308835-999kewdw	304	16	'	'	''
cord-308835-999kewdw	304	17	oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	304	18	spot	spot	NN
cord-308835-999kewdw	304	19	order	order	NN
cord-308835-999kewdw	304	20	for	for	IN
cord-308835-999kewdw	304	21	the	the	DT
cord-308835-999kewdw	304	22	IBV	IBV	NNP
cord-308835-999kewdw	304	23	genome	genome	NN
cord-308835-999kewdw	304	24	.	.	.
cord-308835-999kewdw	305	1	Their	-PRON-	PRP$
cord-308835-999kewdw	305	2	data	datum	NNS
cord-308835-999kewdw	305	3	show	show	VBP
cord-308835-999kewdw	305	4	that	that	IN
cord-308835-999kewdw	305	5	the	the	DT
cord-308835-999kewdw	305	6	IBV	IBV	NNP
cord-308835-999kewdw	305	7	subgenomic	subgenomic	JJ
cord-308835-999kewdw	305	8	RNAs	rna	NNS
cord-308835-999kewdw	305	9	form	form	VBP
cord-308835-999kewdw	305	10	a	a	DT
cord-308835-999kewdw	305	11	nested	nested	JJ
cord-308835-999kewdw	305	12	set	set	NN
cord-308835-999kewdw	305	13	with	with	IN
cord-308835-999kewdw	305	14	common	common	JJ
cord-308835-999kewdw	305	15	3	3	CD
cord-308835-999kewdw	305	16	'	'	''
cord-308835-999kewdw	305	17	ends	end	NNS
cord-308835-999kewdw	305	18	.	.	.
cord-308835-999kewdw	306	1	Taken	take	VBN
cord-308835-999kewdw	306	2	together	together	RB
cord-308835-999kewdw	306	3	,	,	,
cord-308835-999kewdw	306	4	these	these	DT
cord-308835-999kewdw	306	5	data	datum	NNS
cord-308835-999kewdw	306	6	suggest	suggest	VBP
cord-308835-999kewdw	306	7	that	that	IN
cord-308835-999kewdw	306	8	the	the	DT
cord-308835-999kewdw	306	9	MHV	MHV	NNP
cord-308835-999kewdw	306	10	-	-	HYPH
cord-308835-999kewdw	306	11	specific	specific	JJ
cord-308835-999kewdw	306	12	subgenomic	subgenomic	JJ
cord-308835-999kewdw	306	13	RNAs	rna	NNS
cord-308835-999kewdw	306	14	map	map	VBP
cord-308835-999kewdw	306	15	from	from	IN
cord-308835-999kewdw	306	16	the	the	DT
cord-308835-999kewdw	306	17	3	3	CD
cord-308835-999kewdw	306	18	'	'	NN
cord-308835-999kewdw	306	19	end	end	NN
cord-308835-999kewdw	306	20	of	of	IN
cord-308835-999kewdw	306	21	the	the	DT
cord-308835-999kewdw	306	22	genome	genome	NN
cord-308835-999kewdw	306	23	in	in	IN
cord-308835-999kewdw	306	24	a	a	DT
cord-308835-999kewdw	306	25	similar	similar	JJ
cord-308835-999kewdw	306	26	manner	manner	NN
cord-308835-999kewdw	306	27	to	to	IN
cord-308835-999kewdw	306	28	IBV	IBV	NNP
cord-308835-999kewdw	306	29	.	.	.
cord-308835-999kewdw	307	1	A	a	DT
cord-308835-999kewdw	307	2	possible	possible	JJ
cord-308835-999kewdw	307	3	model	model	NN
cord-308835-999kewdw	307	4	of	of	IN
cord-308835-999kewdw	307	5	this	this	DT
cord-308835-999kewdw	307	6	arrangement	arrangement	NN
cord-308835-999kewdw	307	7	is	be	VBZ
cord-308835-999kewdw	307	8	shown	show	VBN
cord-308835-999kewdw	307	9	in	in	IN
cord-308835-999kewdw	307	10	Fig	Fig	NNP
cord-308835-999kewdw	307	11	.	.	.
cord-308835-999kewdw	308	1	9	9	LS
cord-308835-999kewdw	308	2	.	.	.
cord-308835-999kewdw	309	1	It	-PRON-	PRP
cord-308835-999kewdw	309	2	is	be	VBZ
cord-308835-999kewdw	309	3	unlikely	unlikely	JJ
cord-308835-999kewdw	309	4	that	that	IN
cord-308835-999kewdw	309	5	any	any	DT
cord-308835-999kewdw	309	6	of	of	IN
cord-308835-999kewdw	309	7	the	the	DT
cord-308835-999kewdw	309	8	six	six	CD
cord-308835-999kewdw	309	9	subgenomic	subgenomic	NNP
cord-308835-999kewdw	309	10	RNAs	rna	NNS
cord-308835-999kewdw	309	11	are	be	VBP
cord-308835-999kewdw	309	12	defective	defective	JJ
cord-308835-999kewdw	309	13	interfering	interfere	VBG
cord-308835-999kewdw	309	14	RNAs	rna	NNS
cord-308835-999kewdw	309	15	.	.	.
cord-308835-999kewdw	310	1	The	the	DT
cord-308835-999kewdw	310	2	stock	stock	NN
cord-308835-999kewdw	310	3	virus	virus	NN
cord-308835-999kewdw	310	4	used	use	VBN
cord-308835-999kewdw	310	5	in	in	IN
cord-308835-999kewdw	310	6	these	these	DT
cord-308835-999kewdw	310	7	experiments	experiment	NNS
cord-308835-999kewdw	310	8	was	be	VBD
cord-308835-999kewdw	310	9	3	3	CD
cord-308835-999kewdw	310	10	passages	passage	NNS
cord-308835-999kewdw	310	11	(	(	-LRB-
cord-308835-999kewdw	310	12	A59V	A59V	NNP
cord-308835-999kewdw	310	13	)	)	-RRB-
cord-308835-999kewdw	310	14	or	or	CC
cord-308835-999kewdw	310	15	4	4	CD
cord-308835-999kewdw	310	16	passages	passage	NNS
cord-308835-999kewdw	310	17	(	(	-LRB-
cord-308835-999kewdw	310	18	JHMV	JHMV	NNP
cord-308835-999kewdw	310	19	)	)	-RRB-
cord-308835-999kewdw	310	20	removed	remove	VBD
cord-308835-999kewdw	310	21	from	from	IN
cord-308835-999kewdw	310	22	cloning	clone	VBG
cord-308835-999kewdw	310	23	by	by	IN
cord-308835-999kewdw	310	24	limiting	limit	VBG
cord-308835-999kewdw	310	25	dilution	dilution	NN
cord-308835-999kewdw	310	26	(	(	-LRB-
cord-308835-999kewdw	310	27	Robb	Robb	NNP
cord-308835-999kewdw	310	28	and	and	CC
cord-308835-999kewdw	310	29	Bond	Bond	NNP
cord-308835-999kewdw	310	30	,	,	,
cord-308835-999kewdw	310	31	1979b	1979b	CD
cord-308835-999kewdw	310	32	)	)	-RRB-
cord-308835-999kewdw	310	33	.	.	.
cord-308835-999kewdw	311	1	The	the	DT
cord-308835-999kewdw	311	2	low	low	JJ
cord-308835-999kewdw	311	3	m.o.i	m.o.i	NN
cord-308835-999kewdw	311	4	.	.	.
cord-308835-999kewdw	311	5	used	use	VBN
cord-308835-999kewdw	311	6	to	to	TO
cord-308835-999kewdw	311	7	grow	grow	VB
cord-308835-999kewdw	311	8	virus	virus	NN
cord-308835-999kewdw	311	9	stocks	stock	NNS
cord-308835-999kewdw	311	10	(	(	-LRB-
cord-308835-999kewdw	311	11	10m4	10m4	CD
cord-308835-999kewdw	311	12	PFU	pfu	NN
cord-308835-999kewdw	311	13	/	/	SYM
cord-308835-999kewdw	311	14	cell	cell	NN
cord-308835-999kewdw	311	15	)	)	-RRB-
cord-308835-999kewdw	311	16	and	and	CC
cord-308835-999kewdw	311	17	to	to	TO
cord-308835-999kewdw	311	18	initiate	initiate	VB
cord-308835-999kewdw	311	19	infections	infection	NNS
cord-308835-999kewdw	311	20	in	in	IN
cord-308835-999kewdw	311	21	these	these	DT
cord-308835-999kewdw	311	22	experiments	experiment	NNS
cord-308835-999kewdw	311	23	(	(	-LRB-
cord-308835-999kewdw	311	24	0.1	0.1	CD
cord-308835-999kewdw	311	25	-	-	SYM
cord-308835-999kewdw	311	26	0.15	0.15	CD
cord-308835-999kewdw	311	27	PFU	pfu	NN
cord-308835-999kewdw	311	28	/	/	SYM
cord-308835-999kewdw	311	29	cell	cell	NN
cord-308835-999kewdw	311	30	)	)	-RRB-
cord-308835-999kewdw	311	31	selects	select	VBZ
cord-308835-999kewdw	311	32	against	against	IN
cord-308835-999kewdw	311	33	the	the	DT
cord-308835-999kewdw	311	34	generation	generation	NN
cord-308835-999kewdw	311	35	of	of	IN
cord-308835-999kewdw	311	36	defective	defective	JJ
cord-308835-999kewdw	311	37	interfering	interfere	VBG
cord-308835-999kewdw	311	38	particles	particle	NNS
cord-308835-999kewdw	311	39	.	.	.
cord-308835-999kewdw	312	1	Furthermore	furthermore	RB
cord-308835-999kewdw	312	2	,	,	,
cord-308835-999kewdw	312	3	after	after	IN
cord-308835-999kewdw	312	4	10	10	CD
cord-308835-999kewdw	312	5	serial	serial	JJ
cord-308835-999kewdw	312	6	undiluted	undiluted	JJ
cord-308835-999kewdw	312	7	passages	passage	NNS
cord-308835-999kewdw	312	8	of	of	IN
cord-308835-999kewdw	312	9	A59V	a59v	NN
cord-308835-999kewdw	312	10	and	and	CC
cord-308835-999kewdw	312	11	JHMV	JHMV	NNP
cord-308835-999kewdw	312	12	in	in	IN
cord-308835-999kewdw	312	13	17CL	17cl	CD
cord-308835-999kewdw	312	14	.	.	.
cord-308835-999kewdw	313	1	1	1	CD
cord-308835-999kewdw	313	2	cells	cell	NNS
cord-308835-999kewdw	313	3	,	,	,
cord-308835-999kewdw	313	4	the	the	DT
cord-308835-999kewdw	313	5	RNA	RNA	NNP
cord-308835-999kewdw	313	6	gel	gel	NN
cord-308835-999kewdw	313	7	pattern	pattern	NN
cord-308835-999kewdw	313	8	observed	observe	VBN
cord-308835-999kewdw	313	9	is	be	VBZ
cord-308835-999kewdw	313	10	unchanged	unchanged	JJ
cord-308835-999kewdw	313	11	from	from	IN
cord-308835-999kewdw	313	12	early	early	JJ
cord-308835-999kewdw	313	13	passage	passage	NN
cord-308835-999kewdw	313	14	virus	virus	NN
cord-308835-999kewdw	314	1	(	(	-LRB-
cord-308835-999kewdw	314	2	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	314	3	,	,	,
cord-308835-999kewdw	314	4	unpublished	unpublished	JJ
cord-308835-999kewdw	314	5	data	datum	NNS
cord-308835-999kewdw	314	6	)	)	-RRB-
cord-308835-999kewdw	314	7	.	.	.
cord-308835-999kewdw	315	1	The	the	DT
cord-308835-999kewdw	315	2	six	six	CD
cord-308835-999kewdw	315	3	subgenomic	subgenomic	JJ
cord-308835-999kewdw	315	4	RNAs	rna	NNS
cord-308835-999kewdw	315	5	are	be	VBP
cord-308835-999kewdw	315	6	almost	almost	RB
cord-308835-999kewdw	315	7	certainly	certainly	RB
cord-308835-999kewdw	315	8	mRNAs	mrnas	CD
cord-308835-999kewdw	315	9	.	.	.
cord-308835-999kewdw	316	1	They	-PRON-	PRP
cord-308835-999kewdw	316	2	are	be	VBP
cord-308835-999kewdw	316	3	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	316	4	,	,	,
cord-308835-999kewdw	316	5	as	as	IN
cord-308835-999kewdw	316	6	expected	expect	VBN
cord-308835-999kewdw	316	7	of	of	IN
cord-308835-999kewdw	316	8	mRNAs	mrnas	CD
cord-308835-999kewdw	316	9	,	,	,
cord-308835-999kewdw	316	10	and	and	CC
cord-308835-999kewdw	316	11	Robb	Robb	NNP
cord-308835-999kewdw	316	12	and	and	CC
cord-308835-999kewdw	316	13	Bond	Bond	NNP
cord-308835-999kewdw	316	14	(	(	-LRB-
cord-308835-999kewdw	316	15	1979	1979	CD
cord-308835-999kewdw	316	16	)	)	-RRB-
cord-308835-999kewdw	316	17	have	have	VBP
cord-308835-999kewdw	316	18	shown	show	VBN
cord-308835-999kewdw	316	19	that	that	IN
cord-308835-999kewdw	316	20	multiple	multiple	JJ
cord-308835-999kewdw	316	21	MHV	mhv	NN
cord-308835-999kewdw	316	22	-	-	HYPH
cord-308835-999kewdw	316	23	specific	specific	JJ
cord-308835-999kewdw	316	24	RNA	RNA	NNP
cord-308835-999kewdw	316	25	species	specie	NNS
cord-308835-999kewdw	316	26	spanning	span	VBG
cord-308835-999kewdw	316	27	the	the	DT
cord-308835-999kewdw	316	28	size	size	NN
cord-308835-999kewdw	316	29	range	range	NN
cord-308835-999kewdw	316	30	of	of	IN
cord-308835-999kewdw	316	31	the	the	DT
cord-308835-999kewdw	316	32	subgenomic	subgenomic	NNP
cord-308835-999kewdw	316	33	RNAs	RNAs	NNPS
cord-308835-999kewdw	316	34	2	2	CD
cord-308835-999kewdw	316	35	-	-	SYM
cord-308835-999kewdw	316	36	7	7	CD
cord-308835-999kewdw	316	37	are	be	VBP
cord-308835-999kewdw	316	38	present	present	JJ
cord-308835-999kewdw	316	39	on	on	IN
cord-308835-999kewdw	316	40	polysomes	polysome	NNS
cord-308835-999kewdw	316	41	of	of	IN
cord-308835-999kewdw	316	42	infected	infected	JJ
cord-308835-999kewdw	316	43	cells	cell	NNS
cord-308835-999kewdw	316	44	.	.	.
cord-308835-999kewdw	317	1	Furthermore	furthermore	RB
cord-308835-999kewdw	317	2	,	,	,
cord-308835-999kewdw	317	3	Siddell	Siddell	NNP
cord-308835-999kewdw	317	4	et	et	FW
cord-308835-999kewdw	317	5	al	al	NNP
cord-308835-999kewdw	317	6	.	.	.
cord-308835-999kewdw	318	1	(	(	-LRB-
cord-308835-999kewdw	318	2	1980	1980	CD
cord-308835-999kewdw	318	3	)	)	-RRB-
cord-308835-999kewdw	318	4	have	have	VBP
cord-308835-999kewdw	318	5	recently	recently	RB
cord-308835-999kewdw	318	6	demonstrated	demonstrate	VBN
cord-308835-999kewdw	318	7	that	that	IN
cord-308835-999kewdw	318	8	polyadenylated	polyadenylate	VBN
cord-308835-999kewdw	318	9	RNA	RNA	NNP
cord-308835-999kewdw	318	10	isolated	isolate	VBN
cord-308835-999kewdw	318	11	from	from	IN
cord-308835-999kewdw	318	12	JHMV	JHMV	NNP
cord-308835-999kewdw	318	13	-	-	HYPH
cord-308835-999kewdw	318	14	infected	infect	VBN
cord-308835-999kewdw	318	15	cells	cell	NNS
cord-308835-999kewdw	318	16	can	can	MD
cord-308835-999kewdw	318	17	be	be	VB
cord-308835-999kewdw	318	18	translated	translate	VBN
cord-308835-999kewdw	318	19	in	in	IN
cord-308835-999kewdw	318	20	vitro	vitro	FW
cord-308835-999kewdw	318	21	.	.	.
cord-308835-999kewdw	319	1	Using	use	VBG
cord-308835-999kewdw	319	2	sucrose	sucrose	NN
cord-308835-999kewdw	319	3	gradients	gradient	NNS
cord-308835-999kewdw	319	4	they	-PRON-	PRP
cord-308835-999kewdw	319	5	were	be	VBD
cord-308835-999kewdw	319	6	able	able	JJ
cord-308835-999kewdw	319	7	to	to	TO
cord-308835-999kewdw	319	8	partially	partially	RB
cord-308835-999kewdw	319	9	resolve	resolve	VB
cord-308835-999kewdw	319	10	1'7	1'7	CD
cord-308835-999kewdw	319	11	and	and	CC
cord-308835-999kewdw	319	12	19	19	CD
cord-308835-999kewdw	319	13	S	S	NNP
cord-308835-999kewdw	319	14	JHMV	JHMV	NNP
cord-308835-999kewdw	319	15	-	-	HYPH
cord-308835-999kewdw	319	16	specific	specific	JJ
cord-308835-999kewdw	319	17	RNA	RNA	NNP
cord-308835-999kewdw	319	18	species	specie	NNS
cord-308835-999kewdw	319	19	.	.	.
cord-308835-999kewdw	320	1	Their	-PRON-	PRP$
cord-308835-999kewdw	320	2	in	in	IN
cord-308835-999kewdw	320	3	vitro	vitro	FW
cord-308835-999kewdw	320	4	translation	translation	NN
cord-308835-999kewdw	320	5	studies	study	NNS
cord-308835-999kewdw	320	6	suggest	suggest	VBP
cord-308835-999kewdw	320	7	that	that	IN
cord-308835-999kewdw	320	8	the	the	DT
cord-308835-999kewdw	320	9	17	17	CD
cord-308835-999kewdw	320	10	S	S	NNP
cord-308835-999kewdw	320	11	RNA	RNA	NNP
cord-308835-999kewdw	320	12	codes	code	NNS
cord-308835-999kewdw	320	13	for	for	IN
cord-308835-999kewdw	320	14	the	the	DT
cord-308835-999kewdw	320	15	60,000-dalton	60,000-dalton	CD
cord-308835-999kewdw	320	16	virion	virion	NN
cord-308835-999kewdw	320	17	nucleocapsid	nucleocapsid	NN
cord-308835-999kewdw	320	18	protein	protein	NN
cord-308835-999kewdw	320	19	(	(	-LRB-
cord-308835-999kewdw	320	20	Sturman	Sturman	NNP
cord-308835-999kewdw	320	21	,	,	,
cord-308835-999kewdw	320	22	1977	1977	CD
cord-308835-999kewdw	320	23	;	;	:
cord-308835-999kewdw	320	24	Wege	Wege	NNP
cord-308835-999kewdw	320	25	et	et	NNP
cord-308835-999kewdw	320	26	al	al	NNP
cord-308835-999kewdw	320	27	.	.	NNP
cord-308835-999kewdw	320	28	,	,	,
cord-308835-999kewdw	320	29	1979	1979	CD
cord-308835-999kewdw	320	30	)	)	-RRB-
cord-308835-999kewdw	320	31	,	,	,
cord-308835-999kewdw	320	32	while	while	IN
cord-308835-999kewdw	320	33	the	the	DT
cord-308835-999kewdw	320	34	19	19	CD
cord-308835-999kewdw	320	35	S	S	NNP
cord-308835-999kewdw	320	36	RNA	RNA	NNP
cord-308835-999kewdw	320	37	codes	code	NNS
cord-308835-999kewdw	320	38	for	for	IN
cord-308835-999kewdw	320	39	the	the	DT
cord-308835-999kewdw	320	40	23,000-dalton	23,000-dalton	CD
cord-308835-999kewdw	320	41	virion	virion	NN
cord-308835-999kewdw	320	42	glycoprotein	glycoprotein	NN
cord-308835-999kewdw	320	43	(	(	-LRB-
cord-308835-999kewdw	320	44	Sturman	Sturman	NNP
cord-308835-999kewdw	320	45	,	,	,
cord-308835-999kewdw	320	46	1977	1977	CD
cord-308835-999kewdw	320	47	;	;	:
cord-308835-999kewdw	320	48	Wege	Wege	NNP
cord-308835-999kewdw	320	49	et	et	NNP
cord-308835-999kewdw	320	50	al	al	NNP
cord-308835-999kewdw	320	51	.	.	NNP
cord-308835-999kewdw	320	52	,	,	,
cord-308835-999kewdw	320	53	1979	1979	CD
cord-308835-999kewdw	320	54	)	)	-RRB-
cord-308835-999kewdw	320	55	.	.	.
cord-308835-999kewdw	321	1	The	the	DT
cord-308835-999kewdw	321	2	relatively	relatively	RB
cord-308835-999kewdw	321	3	large	large	JJ
cord-308835-999kewdw	321	4	amounts	amount	NNS
cord-308835-999kewdw	321	5	of	of	IN
cord-308835-999kewdw	321	6	these	these	DT
cord-308835-999kewdw	321	7	two	two	CD
cord-308835-999kewdw	321	8	RNAs	rna	NNS
cord-308835-999kewdw	321	9	and	and	CC
cord-308835-999kewdw	321	10	their	-PRON-	PRP$
cord-308835-999kewdw	321	11	sedimentation	sedimentation	NN
cord-308835-999kewdw	321	12	properties	property	NNS
cord-308835-999kewdw	321	13	suggest	suggest	VBP
cord-308835-999kewdw	321	14	that	that	IN
cord-308835-999kewdw	321	15	they	-PRON-	PRP
cord-308835-999kewdw	321	16	correspond	correspond	VBP
cord-308835-999kewdw	321	17	to	to	IN
cord-308835-999kewdw	321	18	RNAs	rna	NNS
cord-308835-999kewdw	321	19	6	6	CD
cord-308835-999kewdw	321	20	and	and	CC
cord-308835-999kewdw	321	21	7	7	CD
cord-308835-999kewdw	321	22	.	.	.
cord-308835-999kewdw	322	1	This	this	DT
cord-308835-999kewdw	322	2	has	have	VBZ
cord-308835-999kewdw	322	3	recently	recently	RB
cord-308835-999kewdw	322	4	been	be	VBN
cord-308835-999kewdw	322	5	confirmed	confirm	VBN
cord-308835-999kewdw	322	6	by	by	IN
cord-308835-999kewdw	322	7	the	the	DT
cord-308835-999kewdw	322	8	in	in	FW
cord-308835-999kewdw	322	9	vitro	vitro	FW
cord-308835-999kewdw	322	10	translation	translation	NN
cord-308835-999kewdw	322	11	of	of	IN
cord-308835-999kewdw	322	12	gel	gel	NN
cord-308835-999kewdw	322	13	purified	purify	VBN
cord-308835-999kewdw	322	14	RNA	RNA	NNP
cord-308835-999kewdw	322	15	(	(	-LRB-
cord-308835-999kewdw	322	16	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	322	17	and	and	CC
cord-308835-999kewdw	322	18	Weiss	Weiss	NNP
cord-308835-999kewdw	322	19	,	,	,
cord-308835-999kewdw	322	20	in	in	IN
cord-308835-999kewdw	322	21	press	press	NN
cord-308835-999kewdw	322	22	;	;	:
cord-308835-999kewdw	322	23	van	van	NNP
cord-308835-999kewdw	322	24	der	der	NNP
cord-308835-999kewdw	322	25	Zeijst	Zeijst	NNP
cord-308835-999kewdw	322	26	,	,	,
cord-308835-999kewdw	322	27	in	in	IN
cord-308835-999kewdw	322	28	press	press	NN
cord-308835-999kewdw	322	29	)	)	-RRB-
cord-308835-999kewdw	322	30	.	.	.
cord-308835-999kewdw	323	1	Van	Van	NNP
cord-308835-999kewdw	323	2	der	der	NNP
cord-308835-999kewdw	323	3	Zeijst	Zeijst	NNP
cord-308835-999kewdw	323	4	has	have	VBZ
cord-308835-999kewdw	323	5	also	also	RB
cord-308835-999kewdw	323	6	demonstrated	demonstrate	VBN
cord-308835-999kewdw	323	7	that	that	IN
cord-308835-999kewdw	323	8	RNA3	RNA3	NNP
cord-308835-999kewdw	323	9	codes	code	NNS
cord-308835-999kewdw	323	10	for	for	IN
cord-308835-999kewdw	323	11	the	the	DT
cord-308835-999kewdw	323	12	150,000-dalton	150,000-dalton	CD
cord-308835-999kewdw	323	13	protein	protein	NN
cord-308835-999kewdw	323	14	described	describe	VBN
cord-308835-999kewdw	323	15	by	by	IN
cord-308835-999kewdw	323	16	Bond	Bond	NNP
cord-308835-999kewdw	323	17	et	et	NNP
cord-308835-999kewdw	323	18	al	al	NNP
cord-308835-999kewdw	323	19	.	.	.
cord-308835-999kewdw	324	1	(	(	-LRB-
cord-308835-999kewdw	324	2	1979	1979	CD
cord-308835-999kewdw	324	3	)	)	-RRB-
cord-308835-999kewdw	324	4	.	.	.
cord-308835-999kewdw	325	1	This	this	DT
cord-308835-999kewdw	325	2	protein	protein	NN
cord-308835-999kewdw	325	3	is	be	VBZ
cord-308835-999kewdw	325	4	the	the	DT
cord-308835-999kewdw	325	5	intracellular	intracellular	JJ
cord-308835-999kewdw	325	6	counterpart	counterpart	NN
cord-308835-999kewdw	325	7	to	to	IN
cord-308835-999kewdw	325	8	the	the	DT
cord-308835-999kewdw	325	9	peplomer	peplomer	NNP
cord-308835-999kewdw	325	10	protein	protein	NN
cord-308835-999kewdw	325	11	described	describe	VBN
cord-308835-999kewdw	325	12	by	by	IN
cord-308835-999kewdw	325	13	Sturman	Sturman	NNP
cord-308835-999kewdw	325	14	(	(	-LRB-
cord-308835-999kewdw	325	15	1977	1977	CD
cord-308835-999kewdw	325	16	)	)	-RRB-
cord-308835-999kewdw	325	17	and	and	CC
cord-308835-999kewdw	325	18	Sturman	Sturman	NNP
cord-308835-999kewdw	325	19	and	and	CC
cord-308835-999kewdw	325	20	Holmes	Holmes	NNP
cord-308835-999kewdw	325	21	(	(	-LRB-
cord-308835-999kewdw	325	22	1977	1977	CD
cord-308835-999kewdw	325	23	)	)	-RRB-
cord-308835-999kewdw	325	24	.	.	.
cord-308835-999kewdw	326	1	Of	of	IN
cord-308835-999kewdw	326	2	the	the	DT
cord-308835-999kewdw	326	3	five	five	CD
cord-308835-999kewdw	326	4	nonstructural	nonstructural	JJ
cord-308835-999kewdw	326	5	proteins	protein	NNS
cord-308835-999kewdw	326	6	described	describe	VBN
cord-308835-999kewdw	326	7	by	by	IN
cord-308835-999kewdw	326	8	Bond	Bond	NNP
cord-308835-999kewdw	326	9	et	et	NNP
cord-308835-999kewdw	326	10	al	al	NNP
cord-308835-999kewdw	326	11	.	.	.
cord-308835-999kewdw	327	1	(	(	-LRB-
cord-308835-999kewdw	327	2	1979	1979	CD
cord-308835-999kewdw	327	3	)	)	-RRB-
cord-308835-999kewdw	327	4	,	,	,
cord-308835-999kewdw	327	5	the	the	DT
cord-308835-999kewdw	327	6	57,000-and	57,000-and	JJ
cord-308835-999kewdw	327	7	54,000-dalton	54,000-dalton	CD
cord-308835-999kewdw	327	8	proteins	protein	NNS
cord-308835-999kewdw	327	9	have	have	VBP
cord-308835-999kewdw	327	10	been	be	VBN
cord-308835-999kewdw	327	11	shown	show	VBN
cord-308835-999kewdw	327	12	to	to	TO
cord-308835-999kewdw	327	13	have	have	VB
cord-308835-999kewdw	327	14	similar	similar	JJ
cord-308835-999kewdw	327	15	tryptic	tryptic	JJ
cord-308835-999kewdw	327	16	maps	map	NNS
cord-308835-999kewdw	327	17	to	to	IN
cord-308835-999kewdw	327	18	the	the	DT
cord-308835-999kewdw	327	19	nucleocapsid	nucleocapsid	NNP
cord-308835-999kewdw	327	20	protein	protein	NN
cord-308835-999kewdw	327	21	(	(	-LRB-
cord-308835-999kewdw	327	22	Leibowitz	Leibowitz	NNP
cord-308835-999kewdw	327	23	,	,	,
cord-308835-999kewdw	327	24	unpublished	unpublished	JJ
cord-308835-999kewdw	327	25	data	datum	NNS
cord-308835-999kewdw	327	26	)	)	-RRB-
cord-308835-999kewdw	327	27	and	and	CC
cord-308835-999kewdw	327	28	are	be	VBP
cord-308835-999kewdw	327	29	presumably	presumably	RB
cord-308835-999kewdw	327	30	translated	translate	VBN
cord-308835-999kewdw	327	31	from	from	IN
cord-308835-999kewdw	327	32	the	the	DT
cord-308835-999kewdw	327	33	same	same	JJ
cord-308835-999kewdw	327	34	mRNA	mrna	NN
cord-308835-999kewdw	327	35	.	.	.
cord-308835-999kewdw	328	1	This	this	DT
cord-308835-999kewdw	328	2	leaves	leave	VBZ
cord-308835-999kewdw	328	3	the	the	DT
cord-308835-999kewdw	328	4	three	three	CD
cord-308835-999kewdw	328	5	other	other	JJ
cord-308835-999kewdw	328	6	nonstructural	nonstructural	JJ
cord-308835-999kewdw	328	7	proteins	protein	NNS
cord-308835-999kewdw	328	8	described	describe	VBN
cord-308835-999kewdw	328	9	by	by	IN
cord-308835-999kewdw	328	10	Bond	Bond	NNP
cord-308835-999kewdw	328	11	et	et	NNP
cord-308835-999kewdw	328	12	al	al	NNP
cord-308835-999kewdw	328	13	.	.	.
cord-308835-999kewdw	329	1	(	(	-LRB-
cord-308835-999kewdw	329	2	1979	1979	CD
cord-308835-999kewdw	329	3	)	)	-RRB-
cord-308835-999kewdw	329	4	and	and	CC
cord-308835-999kewdw	329	5	possibly	possibly	RB
cord-308835-999kewdw	329	6	a	a	DT
cord-308835-999kewdw	329	7	fourth	fourth	JJ
cord-308835-999kewdw	329	8	nonstructural	nonstructural	JJ
cord-308835-999kewdw	329	9	protein	protein	NN
cord-308835-999kewdw	329	10	which	which	WDT
cord-308835-999kewdw	329	11	has	have	VBZ
cord-308835-999kewdw	329	12	been	be	VBN
cord-308835-999kewdw	329	13	described	describe	VBN
cord-308835-999kewdw	329	14	by	by	IN
cord-308835-999kewdw	329	15	Siddell	Siddell	NNP
cord-308835-999kewdw	329	16	et	et	FW
cord-308835-999kewdw	329	17	al	al	NNP
cord-308835-999kewdw	329	18	.	.	.
cord-308835-999kewdw	330	1	(	(	-LRB-
cord-308835-999kewdw	330	2	1979	1979	CD
cord-308835-999kewdw	330	3	)	)	-RRB-
cord-308835-999kewdw	330	4	to	to	TO
cord-308835-999kewdw	330	5	be	be	VB
cord-308835-999kewdw	330	6	assigned	assign	VBN
cord-308835-999kewdw	330	7	to	to	IN
cord-308835-999kewdw	330	8	RNAs	rna	NNS
cord-308835-999kewdw	330	9	2	2	CD
cord-308835-999kewdw	330	10	,	,	,
cord-308835-999kewdw	330	11	4	4	CD
cord-308835-999kewdw	330	12	,	,	,
cord-308835-999kewdw	330	13	and	and	CC
cord-308835-999kewdw	330	14	5	5	CD
cord-308835-999kewdw	330	15	.	.	.
cord-308835-999kewdw	331	1	The	the	DT
cord-308835-999kewdw	331	2	in	in	FW
cord-308835-999kewdw	331	3	vitro	vitro	FW
cord-308835-999kewdw	331	4	translation	translation	NN
cord-308835-999kewdw	331	5	of	of	IN
cord-308835-999kewdw	331	6	all	all	PDT
cord-308835-999kewdw	331	7	the	the	DT
cord-308835-999kewdw	331	8	MHV	MHV	NNP
cord-308835-999kewdw	331	9	-	-	HYPH
cord-308835-999kewdw	331	10	specific	specific	JJ
cord-308835-999kewdw	331	11	RNA	RNA	NNP
cord-308835-999kewdw	331	12	species	specie	NNS
cord-308835-999kewdw	331	13	and	and	CC
cord-308835-999kewdw	331	14	appropriate	appropriate	JJ
cord-308835-999kewdw	331	15	characterization	characterization	NN
cord-308835-999kewdw	331	16	of	of	IN
cord-308835-999kewdw	331	17	the	the	DT
cord-308835-999kewdw	331	18	resulting	result	VBG
cord-308835-999kewdw	331	19	products	product	NNS
cord-308835-999kewdw	331	20	will	will	MD
cord-308835-999kewdw	331	21	be	be	VB
cord-308835-999kewdw	331	22	required	require	VBN
cord-308835-999kewdw	331	23	for	for	IN
cord-308835-999kewdw	331	24	completion	completion	NN
cord-308835-999kewdw	331	25	of	of	IN
cord-308835-999kewdw	331	26	these	these	DT
cord-308835-999kewdw	331	27	coding	coding	NN
cord-308835-999kewdw	331	28	assignments	assignment	NNS
cord-308835-999kewdw	331	29	.	.	.
cord-308835-999kewdw	332	1	nomic	nomic	NNP
cord-308835-999kewdw	332	2	26	26	CD
cord-308835-999kewdw	333	1	S	S	NNP
cord-308835-999kewdw	333	2	RNA	RNA	NNP
cord-308835-999kewdw	333	3	of	of	IN
cord-308835-999kewdw	333	4	alphaviruses	alphaviruses	NNP
cord-308835-999kewdw	333	5	is	be	VBZ
cord-308835-999kewdw	333	6	generated	generate	VBN
cord-308835-999kewdw	333	7	in	in	IN
cord-308835-999kewdw	333	8	this	this	DT
cord-308835-999kewdw	333	9	manner	manner	NN
cord-308835-999kewdw	333	10	(	(	-LRB-
cord-308835-999kewdw	333	11	Brzeski	Brzeski	NNP
cord-308835-999kewdw	333	12	and	and	CC
cord-308835-999kewdw	333	13	Kennedy	Kennedy	NNP
cord-308835-999kewdw	333	14	,	,	,
cord-308835-999kewdw	333	15	1978	1978	CD
cord-308835-999kewdw	333	16	)	)	-RRB-
cord-308835-999kewdw	333	17	.	.	.
cord-308835-999kewdw	334	1	The	the	DT
cord-308835-999kewdw	334	2	second	second	JJ
cord-308835-999kewdw	334	3	mechanism	mechanism	NN
cord-308835-999kewdw	334	4	,	,	,
cord-308835-999kewdw	334	5	again	again	RB
cord-308835-999kewdw	334	6	,	,	,
cord-308835-999kewdw	334	7	involves	involve	VBZ
cord-308835-999kewdw	334	8	the	the	DT
cord-308835-999kewdw	334	9	synthesis	synthesis	NN
cord-308835-999kewdw	334	10	of	of	IN
cord-308835-999kewdw	334	11	a	a	DT
cord-308835-999kewdw	334	12	full	full	JJ
cord-308835-999kewdw	334	13	length	length	NN
cord-308835-999kewdw	334	14	negative	negative	JJ
cord-308835-999kewdw	334	15	strand	strand	NN
cord-308835-999kewdw	334	16	copy	copy	NN
cord-308835-999kewdw	334	17	of	of	IN
cord-308835-999kewdw	334	18	the	the	DT
cord-308835-999kewdw	334	19	genome	genome	NN
cord-308835-999kewdw	334	20	.	.	.
cord-308835-999kewdw	335	1	This	this	DT
cord-308835-999kewdw	335	2	is	be	VBZ
cord-308835-999kewdw	335	3	then	then	RB
cord-308835-999kewdw	335	4	transcribed	transcribe	VBN
cord-308835-999kewdw	335	5	to	to	IN
cord-308835-999kewdw	335	6	a	a	DT
cord-308835-999kewdw	335	7	full	full	JJ
cord-308835-999kewdw	335	8	length	length	NN
cord-308835-999kewdw	335	9	positive	positive	JJ
cord-308835-999kewdw	335	10	strand	strand	NN
cord-308835-999kewdw	335	11	RNA	RNA	NNP
cord-308835-999kewdw	335	12	.	.	.
cord-308835-999kewdw	336	1	The	the	DT
cord-308835-999kewdw	336	2	subgenomic	subgenomic	JJ
cord-308835-999kewdw	336	3	RNAs	rna	NNS
cord-308835-999kewdw	336	4	are	be	VBP
cord-308835-999kewdw	336	5	then	then	RB
cord-308835-999kewdw	336	6	derived	derive	VBN
cord-308835-999kewdw	336	7	by	by	IN
cord-308835-999kewdw	336	8	nucleolytic	nucleolytic	JJ
cord-308835-999kewdw	336	9	cleavage	cleavage	NN
cord-308835-999kewdw	336	10	of	of	IN
cord-308835-999kewdw	336	11	this	this	DT
cord-308835-999kewdw	336	12	full	full	JJ
cord-308835-999kewdw	336	13	length	length	NN
cord-308835-999kewdw	336	14	positive	positive	JJ
cord-308835-999kewdw	336	15	strand	strand	NN
cord-308835-999kewdw	336	16	RNA	RNA	NNP
cord-308835-999kewdw	336	17	.	.	.
cord-308835-999kewdw	337	1	In	in	IN
cord-308835-999kewdw	337	2	the	the	DT
cord-308835-999kewdw	337	3	third	third	JJ
cord-308835-999kewdw	337	4	model	model	NN
cord-308835-999kewdw	337	5	,	,	,
cord-308835-999kewdw	337	6	the	the	DT
cord-308835-999kewdw	337	7	subgenomic	subgenomic	NNP
cord-308835-999kewdw	337	8	RNAs	rna	NNS
cord-308835-999kewdw	337	9	are	be	VBP
cord-308835-999kewdw	337	10	each	each	DT
cord-308835-999kewdw	337	11	transcribed	transcribe	VBN
cord-308835-999kewdw	337	12	from	from	IN
cord-308835-999kewdw	337	13	subgenomic	subgenomic	JJ
cord-308835-999kewdw	337	14	negative	negative	JJ
cord-308835-999kewdw	337	15	strand	strand	NN
cord-308835-999kewdw	337	16	templates	template	NNS
cord-308835-999kewdw	337	17	.	.	.
cord-308835-999kewdw	338	1	These	these	DT
cord-308835-999kewdw	338	2	templates	template	NNS
cord-308835-999kewdw	338	3	could	could	MD
cord-308835-999kewdw	338	4	originate	originate	VB
cord-308835-999kewdw	338	5	by	by	IN
cord-308835-999kewdw	338	6	nucleolytic	nucleolytic	JJ
cord-308835-999kewdw	338	7	cleavage	cleavage	NN
cord-308835-999kewdw	338	8	of	of	IN
cord-308835-999kewdw	338	9	genome	genome	NN
cord-308835-999kewdw	338	10	length	length	NN
cord-308835-999kewdw	338	11	negative	negative	JJ
cord-308835-999kewdw	338	12	strand	strand	NN
cord-308835-999kewdw	338	13	RNA	RNA	NNP
cord-308835-999kewdw	338	14	or	or	CC
cord-308835-999kewdw	338	15	by	by	IN
cord-308835-999kewdw	338	16	internal	internal	JJ
cord-308835-999kewdw	338	17	termination	termination	NN
cord-308835-999kewdw	338	18	of	of	IN
cord-308835-999kewdw	338	19	transcription	transcription	NN
cord-308835-999kewdw	338	20	.	.	.
cord-308835-999kewdw	339	1	We	-PRON-	PRP
cord-308835-999kewdw	339	2	have	have	VBP
cord-308835-999kewdw	339	3	been	be	VBN
cord-308835-999kewdw	339	4	unable	unable	JJ
cord-308835-999kewdw	339	5	to	to	TO
cord-308835-999kewdw	339	6	demonstrate	demonstrate	VB
cord-308835-999kewdw	339	7	any	any	DT
cord-308835-999kewdw	339	8	RNase	rnase	NN
cord-308835-999kewdw	339	9	-	-	HYPH
cord-308835-999kewdw	339	10	resistant	resistant	JJ
cord-308835-999kewdw	339	11	,	,	,
cord-308835-999kewdw	339	12	and	and	CC
cord-308835-999kewdw	339	13	therefore	therefore	RB
cord-308835-999kewdw	339	14	presumably	presumably	RB
cord-308835-999kewdw	339	15	double	double	RB
cord-308835-999kewdw	339	16	-	-	HYPH
cord-308835-999kewdw	339	17	stranded	stranded	JJ
cord-308835-999kewdw	339	18	,	,	,
cord-308835-999kewdw	339	19	RNAs	rna	NNS
cord-308835-999kewdw	339	20	in	in	IN
cord-308835-999kewdw	339	21	infected	infected	JJ
cord-308835-999kewdw	339	22	cells	cell	NNS
cord-308835-999kewdw	339	23	.	.	.
cord-308835-999kewdw	340	1	Present	present	JJ
cord-308835-999kewdw	340	2	data	datum	NNS
cord-308835-999kewdw	340	3	are	be	VBP
cord-308835-999kewdw	340	4	not	not	RB
cord-308835-999kewdw	340	5	sufficient	sufficient	JJ
cord-308835-999kewdw	340	6	to	to	TO
cord-308835-999kewdw	340	7	distinguish	distinguish	VB
cord-308835-999kewdw	340	8	between	between	IN
cord-308835-999kewdw	340	9	these	these	DT
cord-308835-999kewdw	340	10	three	three	CD
cord-308835-999kewdw	340	11	models	model	NNS
cord-308835-999kewdw	340	12	or	or	CC
cord-308835-999kewdw	340	13	a	a	DT
cord-308835-999kewdw	340	14	combination	combination	NN
cord-308835-999kewdw	340	15	of	of	IN
cord-308835-999kewdw	340	16	them	-PRON-	PRP
cord-308835-999kewdw	340	17	.	.	.
cord-308835-999kewdw	341	1	Further	further	JJ
cord-308835-999kewdw	341	2	experiments	experiment	NNS
cord-308835-999kewdw	341	3	using	use	VBG
cord-308835-999kewdw	341	4	ultraviolet	ultraviolet	JJ
cord-308835-999kewdw	341	5	mapping	mapping	NN
cord-308835-999kewdw	341	6	of	of	IN
cord-308835-999kewdw	341	7	transcription	transcription	NN
cord-308835-999kewdw	341	8	initiation	initiation	NN
cord-308835-999kewdw	341	9	sites	site	NNS
cord-308835-999kewdw	341	10	and	and	CC
cord-308835-999kewdw	341	11	the	the	DT
cord-308835-999kewdw	341	12	use	use	NN
cord-308835-999kewdw	341	13	of	of	IN
cord-308835-999kewdw	341	14	positive	positive	JJ
cord-308835-999kewdw	341	15	strand	strand	NN
cord-308835-999kewdw	341	16	probes	probe	NNS
cord-308835-999kewdw	341	17	to	to	TO
cord-308835-999kewdw	341	18	identify	identify	VB
cord-308835-999kewdw	341	19	negative	negative	JJ
cord-308835-999kewdw	341	20	strand	strand	NN
cord-308835-999kewdw	341	21	templates	template	NNS
cord-308835-999kewdw	341	22	may	may	MD
cord-308835-999kewdw	341	23	elucidate	elucidate	VB
cord-308835-999kewdw	341	24	the	the	DT
cord-308835-999kewdw	341	25	mechanism	mechanism	NN
cord-308835-999kewdw	341	26	of	of	IN
cord-308835-999kewdw	341	27	transcription	transcription	NN
cord-308835-999kewdw	341	28	of	of	IN
cord-308835-999kewdw	341	29	coronavirus	coronavirus	NN
cord-308835-999kewdw	341	30	-	-	HYPH
cord-308835-999kewdw	341	31	specific	specific	JJ
cord-308835-999kewdw	341	32	RNA	RNA	NNP
cord-308835-999kewdw	341	33	.	.	.
cord-308835-999kewdw	342	1	Synthesis	synthesis	NN
cord-308835-999kewdw	342	2	of	of	IN
cord-308835-999kewdw	342	3	alphavirus	alphavirus	NNP
cord-308835-999kewdw	342	4	-	-	HYPH
cord-308835-999kewdw	342	5	specified	specify	VBN
cord-308835-999kewdw	342	6	RNA	RNA	NNP
cord-308835-999kewdw	342	7	Bovine	bovine	JJ
cord-308835-999kewdw	342	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	342	9	genome	genome	NN
cord-308835-999kewdw	342	10	Intermediates	Intermediates	NNPS
cord-308835-999kewdw	342	11	in	in	IN
cord-308835-999kewdw	342	12	the	the	DT
cord-308835-999kewdw	342	13	synthesis	synthesis	NN
cord-308835-999kewdw	342	14	of	of	IN
cord-308835-999kewdw	342	15	type	type	NN
cord-308835-999kewdw	342	16	2	2	CD
cord-308835-999kewdw	342	17	adenovirus	adenovirus	NN
cord-308835-999kewdw	342	18	deoxyribonucleic	deoxyribonucleic	NN
cord-308835-999kewdw	342	19	acid	acid	NN
cord-308835-999kewdw	342	20	RNA	RNA	NNP
cord-308835-999kewdw	342	21	of	of	IN
cord-308835-999kewdw	342	22	mouse	mouse	NNP
cord-308835-999kewdw	342	23	hepatitis	hepatitis	NNP
cord-308835-999kewdw	342	24	virus	virus	NN
cord-308835-999kewdw	343	1	Enhanced	enhanced	JJ
cord-308835-999kewdw	343	2	autoradiographic	autoradiographic	JJ
cord-308835-999kewdw	343	3	detection	detection	NN
cord-308835-999kewdw	343	4	of	of	IN
cord-308835-999kewdw	343	5	%	%	NN
cord-308835-999kewdw	343	6	P	p	NN
cord-308835-999kewdw	343	7	and	and	CC
cord-308835-999kewdw	343	8	'	'	''
cord-308835-999kewdw	343	9	%	%	NN
cord-308835-999kewdw	344	1	I	-PRON-	PRP
cord-308835-999kewdw	344	2	using	use	VBG
cord-308835-999kewdw	344	3	intensifying	intensify	VBG
cord-308835-999kewdw	344	4	screens	screen	NNS
cord-308835-999kewdw	344	5	and	and	CC
cord-308835-999kewdw	344	6	hypersensitized	hypersensitized	JJ
cord-308835-999kewdw	344	7	film	film	NN
cord-308835-999kewdw	344	8	Sequence	sequence	NN
cord-308835-999kewdw	344	9	studies	study	NNS
cord-308835-999kewdw	344	10	of	of	IN
cord-308835-999kewdw	344	11	poliovirus	poliovirus	NN
cord-308835-999kewdw	344	12	RNA	RNA	NNP
cord-308835-999kewdw	344	13	.	.	.
cord-308835-999kewdw	345	1	IV	IV	NNP
cord-308835-999kewdw	345	2	.	.	.
cord-308835-999kewdw	346	1	Nucleotide	nucleotide	JJ
cord-308835-999kewdw	346	2	sequence	sequence	NN
cord-308835-999kewdw	346	3	complexities	complexity	NNS
cord-308835-999kewdw	346	4	of	of	IN
cord-308835-999kewdw	346	5	poliovirus	poliovirus	NN
cord-308835-999kewdw	346	6	type	type	NN
cord-308835-999kewdw	346	7	1	1	CD
cord-308835-999kewdw	346	8	,	,	,
cord-308835-999kewdw	346	9	type	type	NN
cord-308835-999kewdw	346	10	2	2	CD
cord-308835-999kewdw	346	11	,	,	,
cord-308835-999kewdw	346	12	and	and	CC
cord-308835-999kewdw	346	13	two	two	CD
cord-308835-999kewdw	346	14	type	type	NN
cord-308835-999kewdw	346	15	1	1	CD
cord-308835-999kewdw	346	16	defective	defective	JJ
cord-308835-999kewdw	346	17	interfering	interfere	VBG
cord-308835-999kewdw	346	18	particles	particle	NNS
cord-308835-999kewdw	347	1	RNAsl	rnasl	JJ
cord-308835-999kewdw	347	2	and	and	CC
cord-308835-999kewdw	347	3	fingerprint	fingerprint	NN
cord-308835-999kewdw	347	4	of	of	IN
cord-308835-999kewdw	347	5	the	the	DT
cord-308835-999kewdw	347	6	poliovirus	poliovirus	NN
cord-308835-999kewdw	347	7	type	type	NN
cord-308835-999kewdw	347	8	3	3	CD
cord-308835-999kewdw	347	9	genome	genome	NN
cord-308835-999kewdw	348	1	Murine	murine	JJ
cord-308835-999kewdw	348	2	coronavirus	coronavirus	NN
cord-308835-999kewdw	348	3	RNA	RNA	NNP
cord-308835-999kewdw	348	4	Biological	Biological	NNP
cord-308835-999kewdw	348	5	properties	property	NNS
cord-308835-999kewdw	348	6	of	of	IN
cord-308835-999kewdw	348	7	avian	avian	JJ
cord-308835-999kewdw	348	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	348	9	Genome	Genome	NNP
cord-308835-999kewdw	348	10	of	of	IN
cord-308835-999kewdw	348	11	infectious	infectious	JJ
cord-308835-999kewdw	348	12	bronchitis	bronchitis	NN
cord-308835-999kewdw	348	13	virus	virus	NN
cord-308835-999kewdw	349	1	The	the	DT
cord-308835-999kewdw	349	2	genome	genome	NN
cord-308835-999kewdw	349	3	of	of	IN
cord-308835-999kewdw	349	4	human	human	JJ
cord-308835-999kewdw	349	5	coronavirus	coronavirus	NN
cord-308835-999kewdw	349	6	strain	strain	VB
cord-308835-999kewdw	349	7	2293	2293	CD
cord-308835-999kewdw	349	8	Analysis	Analysis	NNP
cord-308835-999kewdw	349	9	of	of	IN
cord-308835-999kewdw	349	10	single	single	JJ
cord-308835-999kewdw	349	11	-	-	HYPH
cord-308835-999kewdw	349	12	and	and	CC
cord-308835-999kewdw	349	13	double	double	RB
cord-308835-999kewdw	349	14	-	-	HYPH
cord-308835-999kewdw	349	15	stranded	strand	VBN
cord-308835-999kewdw	349	16	nucleic	nucleic	JJ
cord-308835-999kewdw	349	17	acids	acid	NNS
cord-308835-999kewdw	349	18	on	on	IN
cord-308835-999kewdw	349	19	polyacrylamide	polyacrylamide	NN
cord-308835-999kewdw	349	20	and	and	CC
cord-308835-999kewdw	349	21	agarose	agarose	JJ
cord-308835-999kewdw	349	22	gels	gel	NNS
cord-308835-999kewdw	349	23	by	by	IN
cord-308835-999kewdw	349	24	using	use	VBG
cord-308835-999kewdw	349	25	glyoxal	glyoxal	NNP
cord-308835-999kewdw	349	26	and	and	CC
cord-308835-999kewdw	349	27	acridine	acridine	NNP
cord-308835-999kewdw	349	28	orange	orange	NNP
cord-308835-999kewdw	349	29	Ribonucleic	Ribonucleic	NNP
cord-308835-999kewdw	349	30	acid	acid	NN
cord-308835-999kewdw	349	31	synthesis	synthesis	NN
cord-308835-999kewdw	349	32	in	in	IN
cord-308835-999kewdw	349	33	porcine	porcine	JJ
cord-308835-999kewdw	349	34	cell	cell	NN
cord-308835-999kewdw	349	35	cultures	culture	NNS
cord-308835-999kewdw	349	36	infected	infect	VBN
cord-308835-999kewdw	349	37	with	with	IN
cord-308835-999kewdw	349	38	transmissible	transmissible	JJ
cord-308835-999kewdw	349	39	gastroenteritis	gastroenteritis	NN
cord-308835-999kewdw	349	40	virus	virus	NN
cord-308835-999kewdw	349	41	Amount	amount	NN
cord-308835-999kewdw	349	42	of	of	IN
cord-308835-999kewdw	349	43	viral	viral	JJ
cord-308835-999kewdw	349	44	DNA	dna	NN
cord-308835-999kewdw	349	45	in	in	IN
cord-308835-999kewdw	349	46	the	the	DT
cord-308835-999kewdw	349	47	genome	genome	NN
cord-308835-999kewdw	349	48	of	of	IN
cord-308835-999kewdw	349	49	cells	cell	NNS
cord-308835-999kewdw	349	50	transformed	transform	VBN
cord-308835-999kewdw	349	51	by	by	IN
cord-308835-999kewdw	349	52	adenovirus	adenovirus	NN
cord-308835-999kewdw	349	53	type	type	NN
cord-308835-999kewdw	349	54	2	2	CD
cord-308835-999kewdw	349	55	Replication	replication	NN
cord-308835-999kewdw	349	56	of	of	IN
cord-308835-999kewdw	349	57	mengovirus	mengovirus	NNP
cord-308835-999kewdw	349	58	.	.	.
cord-308835-999kewdw	350	1	I.	I.	NNP
cord-308835-999kewdw	350	2	Effect	effect	NN
cord-308835-999kewdw	350	3	on	on	IN
cord-308835-999kewdw	350	4	synthesis	synthesis	NN
cord-308835-999kewdw	350	5	of	of	IN
cord-308835-999kewdw	350	6	macromolecules	macromolecule	NNS
cord-308835-999kewdw	350	7	by	by	IN
cord-308835-999kewdw	350	8	host	host	NN
cord-308835-999kewdw	350	9	cell	cell	NN
cord-308835-999kewdw	350	10	Coronaviridae	Coronaviridae	NNP
cord-308835-999kewdw	350	11	.	.	.
cord-308835-999kewdw	351	1	In	in	IN
cord-308835-999kewdw	351	2	"	"	``
cord-308835-999kewdw	351	3	Comprehensive	Comprehensive	NNP
cord-308835-999kewdw	351	4	Virology	Virology	NNP
cord-308835-999kewdw	351	5	Pathogenic	Pathogenic	NNP
cord-308835-999kewdw	351	6	murine	murine	JJ
cord-308835-999kewdw	351	7	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	351	8	.	.	.
cord-308835-999kewdw	352	1	I.	I.	NNP
cord-308835-999kewdw	352	2	Characterization	characterization	NN
cord-308835-999kewdw	352	3	of	of	IN
cord-308835-999kewdw	352	4	biological	biological	JJ
cord-308835-999kewdw	352	5	behavior	behavior	NN
cord-308835-999kewdw	352	6	in	in	IN
cord-308835-999kewdw	352	7	vitro	vitro	FW
cord-308835-999kewdw	352	8	and	and	CC
cord-308835-999kewdw	352	9	virus	virus	NN
cord-308835-999kewdw	352	10	specific	specific	JJ
cord-308835-999kewdw	352	11	intracellular	intracellular	JJ
cord-308835-999kewdw	352	12	RNA	RNA	NNP
cord-308835-999kewdw	352	13	of	of	IN
cord-308835-999kewdw	352	14	strongly	strongly	RB
cord-308835-999kewdw	352	15	neurotropic	neurotropic	NNP
cord-308835-999kewdw	352	16	JHMV	JHMV	NNP
cord-308835-999kewdw	352	17	and	and	CC
cord-308835-999kewdw	352	18	weakly	weakly	RB
cord-308835-999kewdw	352	19	neurotropic	neurotropic	NNP
cord-308835-999kewdw	352	20	A59V	A59V	NNP
cord-308835-999kewdw	352	21	viruses	virus	NNS
cord-308835-999kewdw	352	22	Pathogenic	pathogenic	JJ
cord-308835-999kewdw	352	23	murine	murine	JJ
cord-308835-999kewdw	352	24	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	352	25	.	.	.
cord-308835-999kewdw	353	1	III	III	NNP
cord-308835-999kewdw	353	2	.	.	.
cord-308835-999kewdw	354	1	Biological	biological	JJ
cord-308835-999kewdw	354	2	and	and	CC
cord-308835-999kewdw	354	3	biochemical	biochemical	JJ
cord-308835-999kewdw	354	4	characterization	characterization	NN
cord-308835-999kewdw	354	5	of	of	IN
cord-308835-999kewdw	354	6	temperature	temperature	NN
cord-308835-999kewdw	354	7	sensitive	sensitive	JJ
cord-308835-999kewdw	354	8	mutants	mutant	NNS
cord-308835-999kewdw	354	9	of	of	IN
cord-308835-999kewdw	354	10	JHMV	JHMV	NNP
cord-308835-999kewdw	354	11	197'7	197'7	CD
cord-308835-999kewdw	354	12	)	)	-RRB-
cord-308835-999kewdw	354	13	.	.	.
cord-308835-999kewdw	355	1	Presence	presence	NN
cord-308835-999kewdw	355	2	of	of	IN
cord-308835-999kewdw	355	3	infectious	infectious	JJ
cord-308835-999kewdw	355	4	polyadenylated	polyadenylated	JJ
cord-308835-999kewdw	355	5	RNA	RNA	NNP
cord-308835-999kewdw	355	6	in	in	IN
cord-308835-999kewdw	355	7	the	the	DT
cord-308835-999kewdw	355	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	355	9	avian	avian	JJ
cord-308835-999kewdw	355	10	bronchitis	bronchitis	NN
cord-308835-999kewdw	355	11	virus	virus	NN
cord-308835-999kewdw	355	12	Coronavirus	Coronavirus	NNP
cord-308835-999kewdw	355	13	JHM	JHM	NNP
cord-308835-999kewdw	355	14	:	:	:
cord-308835-999kewdw	355	15	Cell	cell	NN
cord-308835-999kewdw	355	16	-	-	HYPH
cord-308835-999kewdw	355	17	free	free	JJ
cord-308835-999kewdw	355	18	synthesis	synthesis	NN
cord-308835-999kewdw	355	19	of	of	IN
cord-308835-999kewdw	355	20	structural	structural	JJ
cord-308835-999kewdw	355	21	protein	protein	NN
cord-308835-999kewdw	355	22	~60	~60	CD
cord-308835-999kewdw	355	23	Coronavirus	Coronavirus	NNP
cord-308835-999kewdw	355	24	multiplication	multiplication	NN
cord-308835-999kewdw	355	25	strategy	strategy	NN
cord-308835-999kewdw	355	26	.	.	.
cord-308835-999kewdw	356	1	I.	I.	NNP
cord-308835-999kewdw	356	2	Identification	identification	NN
cord-308835-999kewdw	356	3	and	and	CC
cord-308835-999kewdw	356	4	characterization	characterization	NN
cord-308835-999kewdw	356	5	of	of	IN
cord-308835-999kewdw	356	6	virus	virus	NN
cord-308835-999kewdw	356	7	-	-	HYPH
cord-308835-999kewdw	356	8	specified	specify	VBN
cord-308835-999kewdw	356	9	RNA	RNA	NNP
cord-308835-999kewdw	356	10	Coronavirus	Coronavirus	NNP
cord-308835-999kewdw	356	11	multiplication	multiplication	NN
cord-308835-999kewdw	356	12	strategy	strategy	NN
cord-308835-999kewdw	356	13	.	.	.
cord-308835-999kewdw	357	1	II	II	NNP
cord-308835-999kewdw	357	2	.	.	.
cord-308835-999kewdw	358	1	Mapping	map	VBG
cord-308835-999kewdw	358	2	the	the	DT
cord-308835-999kewdw	358	3	avian	avian	JJ
cord-308835-999kewdw	358	4	infectious	infectious	JJ
cord-308835-999kewdw	358	5	bronchitis	bronchitis	NN
cord-308835-999kewdw	358	6	virus	virus	NN
cord-308835-999kewdw	358	7	intracellular	intracellular	JJ
cord-308835-999kewdw	358	8	RNA	RNA	NNP
cord-308835-999kewdw	358	9	species	specie	NNS
cord-308835-999kewdw	358	10	to	to	IN
cord-308835-999kewdw	358	11	the	the	DT
cord-308835-999kewdw	358	12	genome	genome	NN
cord-308835-999kewdw	358	13	Characterization	characterization	NN
cord-308835-999kewdw	358	14	of	of	IN
cord-308835-999kewdw	358	15	a	a	DT
cord-308835-999kewdw	358	16	coronavirus	coronavirus	NN
cord-308835-999kewdw	358	17	.	.	.
cord-308835-999kewdw	359	1	I.	I.	NNP
cord-308835-999kewdw	359	2	Structural	structural	JJ
cord-308835-999kewdw	359	3	proteins	protein	NNS
cord-308835-999kewdw	359	4	:	:	:
cord-308835-999kewdw	359	5	effects	effect	NNS
cord-308835-999kewdw	359	6	of	of	IN
cord-308835-999kewdw	359	7	preparative	preparative	JJ
cord-308835-999kewdw	359	8	conditions	condition	NNS
cord-308835-999kewdw	359	9	on	on	IN
cord-308835-999kewdw	359	10	the	the	DT
cord-308835-999kewdw	359	11	migration	migration	NN
cord-308835-999kewdw	359	12	of	of	IN
cord-308835-999kewdw	359	13	protein	protein	NN
cord-308835-999kewdw	359	14	in	in	IN
cord-308835-999kewdw	360	1	polyacrylamide	polyacrylamide	NN
cord-308835-999kewdw	360	2	gels	gel	NNS
cord-308835-999kewdw	360	3	Enhanced	enhanced	JJ
cord-308835-999kewdw	360	4	growth	growth	NN
cord-308835-999kewdw	360	5	of	of	IN
cord-308835-999kewdw	360	6	a	a	DT
cord-308835-999kewdw	360	7	murine	murine	JJ
cord-308835-999kewdw	360	8	coronavirus	coronavirus	NN
cord-308835-999kewdw	360	9	in	in	IN
cord-308835-999kewdw	360	10	transformed	transform	VBN
cord-308835-999kewdw	360	11	mouse	mouse	NN
cord-308835-999kewdw	360	12	cells	cell	NNS
cord-308835-999kewdw	360	13	Presence	presence	NN
cord-308835-999kewdw	360	14	of	of	IN
cord-308835-999kewdw	360	15	genomic	genomic	JJ
cord-308835-999kewdw	360	16	polyadenylate	polyadenylate	NN
cord-308835-999kewdw	360	17	and	and	CC
cord-308835-999kewdw	360	18	absence	absence	NN
cord-308835-999kewdw	360	19	of	of	IN
cord-308835-999kewdw	360	20	detectable	detectable	JJ
cord-308835-999kewdw	360	21	virion	virion	NN
cord-308835-999kewdw	360	22	transeriptase	transeriptase	NN
cord-308835-999kewdw	360	23	in	in	IN
cord-308835-999kewdw	360	24	human	human	JJ
cord-308835-999kewdw	360	25	coronavirus	coronavirus	NN
cord-308835-999kewdw	360	26	OC-43	OC-43	NNP
cord-308835-999kewdw	360	27	Coronaviridae	Coronaviridae	NNP
cord-308835-999kewdw	360	28	Isolation	isolation	NN
cord-308835-999kewdw	360	29	and	and	CC
cord-308835-999kewdw	360	30	characterization	characterization	NN
cord-308835-999kewdw	360	31	of	of	IN
cord-308835-999kewdw	360	32	the	the	DT
cord-308835-999kewdw	360	33	virus	virus	NN
cord-308835-999kewdw	360	34	RNA	RNA	NNP
cord-308835-999kewdw	360	35	and	and	CC
cord-308835-999kewdw	360	36	the	the	DT
cord-308835-999kewdw	360	37	subgenomic	subgenomic	NNP
cord-308835-999kewdw	360	38	mRNAs	mRNAs	NNPS
cord-308835-999kewdw	360	39	of	of	IN
cord-308835-999kewdw	360	40	mouse	mouse	NNP
cord-308835-999kewdw	360	41	hepatitis	hepatitis	NN
cord-308835-999kewdw	360	42	virus	virus	NN
cord-308835-999kewdw	360	43	MHV	MHV	NNP
cord-308835-999kewdw	360	44	A59	A59	NNP
cord-308835-999kewdw	360	45	Genomic	Genomic	NNP
cord-308835-999kewdw	360	46	RNA	RNA	NNP
cord-308835-999kewdw	360	47	of	of	IN
cord-308835-999kewdw	360	48	the	the	DT
cord-308835-999kewdw	360	49	murine	murine	JJ
cord-308835-999kewdw	360	50	coronavirus	coronavirus	NN
cord-308835-999kewdw	360	51	JHM	JHM	NNP
cord-308835-999kewdw	360	52	Structural	structural	JJ
cord-308835-999kewdw	360	53	polypeptides	polypeptide	NNS
cord-308835-999kewdw	360	54	of	of	IN
cord-308835-999kewdw	360	55	the	the	DT
cord-308835-999kewdw	360	56	murine	murine	JJ
cord-308835-999kewdw	360	57	coronavirus	coronavirus	NN
cord-308835-999kewdw	360	58	JHM	JHM	NNP
cord-308835-999kewdw	360	59	Comparison	comparison	NN
cord-308835-999kewdw	360	60	of	of	IN
cord-308835-999kewdw	360	61	the	the	DT
cord-308835-999kewdw	360	62	RNAs	rna	NNS
cord-308835-999kewdw	360	63	of	of	IN
cord-308835-999kewdw	360	64	murine	murine	JJ
cord-308835-999kewdw	360	65	and	and	CC
cord-308835-999kewdw	360	66	human	human	JJ
cord-308835-999kewdw	360	67	coronaviruses	coronaviruse	NNS
cord-308835-999kewdw	361	1	The	the	DT
cord-308835-999kewdw	361	2	dynamics	dynamic	NNS
cord-308835-999kewdw	361	3	of	of	IN
cord-308835-999kewdw	361	4	maternal	maternal	JJ
cord-308835-999kewdw	361	5	poly	poly	NN
cord-308835-999kewdw	361	6	(	(	-LRB-
cord-308835-999kewdw	361	7	A)-containing	A)-containing	NNP
cord-308835-999kewdw	361	8	mRNA	mrna	NN
cord-308835-999kewdw	361	9	in	in	IN
cord-308835-999kewdw	361	10	fertilized	fertilize	VBN
cord-308835-999kewdw	361	11	sea	sea	NN
cord-308835-999kewdw	361	12	urchin	urchin	NN
cord-308835-999kewdw	361	13	eggs	egg	NNS
cord-308835-999kewdw	361	14	Polyadenylate	Polyadenylate	NNP
cord-308835-999kewdw	361	15	in	in	IN
cord-308835-999kewdw	361	16	the	the	DT
cord-308835-999kewdw	361	17	virion	virion	NN
cord-308835-999kewdw	361	18	RNA	RNA	NNP
cord-308835-999kewdw	361	19	of	of	IN
cord-308835-999kewdw	361	20	mouse	mouse	NNP
cord-308835-999kewdw	361	21	hepatitis	hepatitis	NNP
cord-308835-999kewdw	361	22	virus	virus	NN
cord-308835-999kewdw	362	1	The	the	DT
cord-308835-999kewdw	362	2	authors	author	NNS
cord-308835-999kewdw	362	3	would	would	MD
cord-308835-999kewdw	362	4	like	like	VB
cord-308835-999kewdw	362	5	to	to	TO
cord-308835-999kewdw	362	6	thank	thank	VB
cord-308835-999kewdw	362	7	Dr.	Dr.	NNP
cord-308835-999kewdw	362	8	Ian	Ian	NNP
cord-308835-999kewdw	362	9	Kennedy	Kennedy	NNP
cord-308835-999kewdw	362	10	for	for	IN
cord-308835-999kewdw	362	11	teaching	teach	VBG
cord-308835-999kewdw	362	12	us	-PRON-	PRP
cord-308835-999kewdw	362	13	the	the	DT
cord-308835-999kewdw	362	14	technique	technique	NN
cord-308835-999kewdw	362	15	of	of	IN
cord-308835-999kewdw	362	16	oligonucleotide	oligonucleotide	JJ
cord-308835-999kewdw	362	17	fingerprinting	fingerprinting	NN
cord-308835-999kewdw	362	18	and	and	CC
cord-308835-999kewdw	362	19	the	the	DT
cord-308835-999kewdw	362	20	method	method	NN
cord-308835-999kewdw	362	21	for	for	IN
cord-308835-999kewdw	362	22	recovering	recover	VBG
cord-308835-999kewdw	362	23	RNA	RNA	NNP
cord-308835-999kewdw	362	24	from	from	IN
cord-308835-999kewdw	362	25	agarose	agarose	JJ
cord-308835-999kewdw	362	26	gels	gel	NNS
cord-308835-999kewdw	362	27	.	.	.
cord-308835-999kewdw	363	1	We	-PRON-	PRP
cord-308835-999kewdw	363	2	are	be	VBP
cord-308835-999kewdw	363	3	grateful	grateful	JJ
cord-308835-999kewdw	363	4	to	to	IN
cord-308835-999kewdw	363	5	David	David	NNP
cord-308835-999kewdw	363	6	Stern	Stern	NNP
cord-308835-999kewdw	363	7	and	and	CC
cord-308835-999kewdw	363	8	Dr.	Dr.	NNP
cord-308835-999kewdw	363	9	Kennedy	Kennedy	NNP
cord-308835-999kewdw	363	10	for	for	IN
cord-308835-999kewdw	363	11	making	make	VBG
cord-308835-999kewdw	363	12	their	-PRON-	PRP$
cord-308835-999kewdw	363	13	results	result	NNS
cord-308835-999kewdw	363	14	available	available	JJ
cord-308835-999kewdw	363	15	to	to	IN
cord-308835-999kewdw	363	16	us	-PRON-	PRP
cord-308835-999kewdw	363	17	prior	prior	RB
cord-308835-999kewdw	363	18	to	to	IN
cord-308835-999kewdw	363	19	publication	publication	NN
cord-308835-999kewdw	363	20	.	.	.
cord-308835-999kewdw	364	1	The	the	DT
cord-308835-999kewdw	364	2	encouragement	encouragement	NN
cord-308835-999kewdw	364	3	of	of	IN
cord-308835-999kewdw	364	4	Dr.	Dr.	NNP
cord-308835-999kewdw	364	5	Peter	Peter	NNP
cord-308835-999kewdw	364	6	Lampert	Lampert	NNP
cord-308835-999kewdw	364	7	and	and	CC
cord-308835-999kewdw	364	8	Dr.	Dr.	NNP
cord-308835-999kewdw	364	9	Michael	Michael	NNP
cord-308835-999kewdw	364	10	Oxman	Oxman	NNP
cord-308835-999kewdw	364	11	are	be	VBP
cord-308835-999kewdw	364	12	gratefully	gratefully	RB
cord-308835-999kewdw	364	13	acknowledged	acknowledge	VBN
cord-308835-999kewdw	364	14	.	.	.
cord-308835-999kewdw	365	1	We	-PRON-	PRP
cord-308835-999kewdw	365	2	would	would	MD
cord-308835-999kewdw	365	3	like	like	VB
cord-308835-999kewdw	365	4	to	to	TO
cord-308835-999kewdw	365	5	thank	thank	VB
cord-308835-999kewdw	365	6	Jim	Jim	NNP
cord-308835-999kewdw	365	7	De	De	NNP
cord-308835-999kewdw	365	8	Vries	Vries	NNP
cord-308835-999kewdw	365	9	and	and	CC
cord-308835-999kewdw	365	10	Eric	Eric	NNP
cord-308835-999kewdw	365	11	Paavola	Paavola	NNP
cord-308835-999kewdw	365	12	for	for	IN
cord-308835-999kewdw	365	13	excellent	excellent	JJ
cord-308835-999kewdw	365	14	technical	technical	JJ
cord-308835-999kewdw	365	15	assistance	assistance	NN
cord-308835-999kewdw	365	16	.	.	.
cord-308835-999kewdw	366	1	Finally	finally	RB
cord-308835-999kewdw	366	2	,	,	,
cord-308835-999kewdw	366	3	the	the	DT
cord-308835-999kewdw	366	4	question	question	NN
cord-308835-999kewdw	366	5	arises	arise	VBZ
cord-308835-999kewdw	366	6	about	about	IN
cord-308835-999kewdw	366	7	how	how	WRB
cord-308835-999kewdw	366	8	the	the	DT
cord-308835-999kewdw	366	9	subgenomic	subgenomic	JJ
cord-308835-999kewdw	366	10	RNAs	rna	NNS
cord-308835-999kewdw	366	11	are	be	VBP
cord-308835-999kewdw	366	12	synthesized	synthesize	VBN
cord-308835-999kewdw	366	13	.	.	.
cord-308835-999kewdw	367	1	Three	three	CD
cord-308835-999kewdw	367	2	mechanisms	mechanism	NNS
cord-308835-999kewdw	367	3	are	be	VBP
cord-308835-999kewdw	367	4	possible	possible	JJ
cord-308835-999kewdw	367	5	.	.	.
cord-308835-999kewdw	368	1	In	in	IN
cord-308835-999kewdw	368	2	the	the	DT
cord-308835-999kewdw	368	3	first	first	JJ
cord-308835-999kewdw	368	4	mechanism	mechanism	NN
cord-308835-999kewdw	368	5	,	,	,
cord-308835-999kewdw	368	6	a	a	DT
cord-308835-999kewdw	368	7	full	full	JJ
cord-308835-999kewdw	368	8	length	length	NN
cord-308835-999kewdw	368	9	negative	negative	JJ
cord-308835-999kewdw	368	10	strand	strand	NN
cord-308835-999kewdw	368	11	copy	copy	NN
cord-308835-999kewdw	368	12	is	be	VBZ
cord-308835-999kewdw	368	13	made	make	VBN
cord-308835-999kewdw	368	14	from	from	IN
cord-308835-999kewdw	368	15	the	the	DT
cord-308835-999kewdw	368	16	positive	positive	JJ
cord-308835-999kewdw	368	17	strand	strand	NN
cord-308835-999kewdw	368	18	genomic	genomic	JJ
cord-308835-999kewdw	368	19	RNA	RNA	NNP
cord-308835-999kewdw	368	20	.	.	.
cord-308835-999kewdw	369	1	Each	each	DT
cord-308835-999kewdw	369	2	subgenomic	subgenomic	NNP
cord-308835-999kewdw	369	3	RNA	RNA	NNP
cord-308835-999kewdw	369	4	species	specie	NNS
cord-308835-999kewdw	369	5	is	be	VBZ
cord-308835-999kewdw	369	6	then	then	RB
cord-308835-999kewdw	369	7	separately	separately	RB
cord-308835-999kewdw	369	8	transcribed	transcribe	VBN
cord-308835-999kewdw	369	9	from	from	IN
cord-308835-999kewdw	369	10	this	this	DT
cord-308835-999kewdw	369	11	negative	negative	JJ
cord-308835-999kewdw	369	12	strand	strand	NN
cord-308835-999kewdw	369	13	template	template	NN
cord-308835-999kewdw	369	14	.	.	.
cord-308835-999kewdw	370	1	This	this	DT
cord-308835-999kewdw	370	2	model	model	NN
cord-308835-999kewdw	370	3	would	would	MD
cord-308835-999kewdw	370	4	involve	involve	VB
cord-308835-999kewdw	370	5	internal	internal	JJ
cord-308835-999kewdw	370	6	initiation	initiation	NN
cord-308835-999kewdw	370	7	of	of	IN
cord-308835-999kewdw	370	8	transcription	transcription	NN
cord-308835-999kewdw	370	9	.	.	.
cord-308835-999kewdw	370	10	 	 	_SP