id	sid	tid	token	lemma	pos
cord-326027-58whwspe	1	1	key	key	JJ
cord-326027-58whwspe	2	1	:	:	:
cord-326027-58whwspe	2	2	cord-326027	cord-326027	NNP
cord-326027-58whwspe	2	3	-	-	HYPH
cord-326027-58whwspe	2	4	58whwspe	58whwspe	CD
cord-326027-58whwspe	2	5	authors	author	NNS
cord-326027-58whwspe	2	6	:	:	:
cord-326027-58whwspe	2	7	Hernaez	Hernaez	NNP
cord-326027-58whwspe	2	8	,	,	,
cord-326027-58whwspe	2	9	Bruno	Bruno	NNP
cord-326027-58whwspe	2	10	;	;	:
cord-326027-58whwspe	2	11	Escribano	Escribano	NNP
cord-326027-58whwspe	2	12	,	,	,
cord-326027-58whwspe	2	13	Jose	Jose	NNP
cord-326027-58whwspe	2	14	M.	M.	NNP
cord-326027-58whwspe	2	15	;	;	:
cord-326027-58whwspe	2	16	Alonso	Alonso	NNP
cord-326027-58whwspe	2	17	,	,	,
cord-326027-58whwspe	2	18	Covadonga	Covadonga	NNP
cord-326027-58whwspe	2	19	title	title	NN
cord-326027-58whwspe	2	20	:	:	:
cord-326027-58whwspe	3	1	Visualization	visualization	NN
cord-326027-58whwspe	3	2	of	of	IN
cord-326027-58whwspe	3	3	the	the	DT
cord-326027-58whwspe	3	4	African	african	JJ
cord-326027-58whwspe	3	5	swine	swine	NN
cord-326027-58whwspe	3	6	fever	fever	NN
cord-326027-58whwspe	3	7	virus	virus	NN
cord-326027-58whwspe	3	8	infection	infection	NN
cord-326027-58whwspe	3	9	in	in	IN
cord-326027-58whwspe	3	10	living	live	VBG
cord-326027-58whwspe	3	11	cells	cell	NNS
cord-326027-58whwspe	3	12	by	by	IN
cord-326027-58whwspe	3	13	incorporation	incorporation	NN
cord-326027-58whwspe	3	14	into	into	IN
cord-326027-58whwspe	3	15	the	the	DT
cord-326027-58whwspe	3	16	virus	virus	NN
cord-326027-58whwspe	3	17	particle	particle	NN
cord-326027-58whwspe	3	18	of	of	IN
cord-326027-58whwspe	3	19	green	green	JJ
cord-326027-58whwspe	3	20	fluorescent	fluorescent	JJ
cord-326027-58whwspe	3	21	protein	protein	NN
cord-326027-58whwspe	3	22	-	-	HYPH
cord-326027-58whwspe	3	23	p54	p54	NN
cord-326027-58whwspe	3	24	membrane	membrane	NN
cord-326027-58whwspe	3	25	protein	protein	NN
cord-326027-58whwspe	3	26	chimera	chimera	NN
cord-326027-58whwspe	3	27	date	date	NN
cord-326027-58whwspe	3	28	:	:	:
cord-326027-58whwspe	3	29	2006	2006	CD
cord-326027-58whwspe	3	30	-	-	HYPH
cord-326027-58whwspe	3	31	06	06	CD
cord-326027-58whwspe	3	32	-	-	SYM
cord-326027-58whwspe	3	33	20	20	CD
cord-326027-58whwspe	3	34	journal	journal	NN
cord-326027-58whwspe	3	35	:	:	:
cord-326027-58whwspe	4	1	Virology	virology	NN
cord-326027-58whwspe	4	2	DOI	DOI	NNP
cord-326027-58whwspe	4	3	:	:	:
cord-326027-58whwspe	4	4	10.1016/	10.1016/	CD
cord-326027-58whwspe	5	1	j.virol.2006.01.021	j.virol.2006.01.021	XX
cord-326027-58whwspe	6	1	sha	sha	NNP
cord-326027-58whwspe	6	2	:	:	:
cord-326027-58whwspe	7	1	61fa6a990e2ad4f339c27eeba2b345aae391143e	61fa6a990e2ad4f339c27eeba2b345aae391143e	CD
cord-326027-58whwspe	7	2	doc_id	doc_id	CD
cord-326027-58whwspe	7	3	:	:	:
cord-326027-58whwspe	8	1	326027	326027	CD
cord-326027-58whwspe	8	2	cord_uid	cord_uid	NN
cord-326027-58whwspe	8	3	:	:	:
cord-326027-58whwspe	9	1	58whwspe	58whwspe	CD
cord-326027-58whwspe	10	1	Many	many	JJ
cord-326027-58whwspe	10	2	stages	stage	NNS
cord-326027-58whwspe	10	3	of	of	IN
cord-326027-58whwspe	10	4	African	african	JJ
cord-326027-58whwspe	10	5	swine	swine	NN
cord-326027-58whwspe	10	6	fever	fever	NN
cord-326027-58whwspe	10	7	virus	virus	NN
cord-326027-58whwspe	10	8	infection	infection	NN
cord-326027-58whwspe	10	9	have	have	VBP
cord-326027-58whwspe	10	10	not	not	RB
cord-326027-58whwspe	10	11	yet	yet	RB
cord-326027-58whwspe	10	12	been	be	VBN
cord-326027-58whwspe	10	13	studied	study	VBN
cord-326027-58whwspe	10	14	in	in	IN
cord-326027-58whwspe	10	15	detail	detail	NN
cord-326027-58whwspe	10	16	.	.	.
cord-326027-58whwspe	11	1	To	to	TO
cord-326027-58whwspe	11	2	track	track	VB
cord-326027-58whwspe	11	3	the	the	DT
cord-326027-58whwspe	11	4	behavior	behavior	NN
cord-326027-58whwspe	11	5	of	of	IN
cord-326027-58whwspe	11	6	African	african	JJ
cord-326027-58whwspe	11	7	swine	swine	NN
cord-326027-58whwspe	11	8	fever	fever	NN
cord-326027-58whwspe	11	9	virus	virus	NN
cord-326027-58whwspe	11	10	(	(	-LRB-
cord-326027-58whwspe	11	11	ASFV	ASFV	NNP
cord-326027-58whwspe	11	12	)	)	-RRB-
cord-326027-58whwspe	11	13	in	in	IN
cord-326027-58whwspe	11	14	the	the	DT
cord-326027-58whwspe	11	15	infected	infected	JJ
cord-326027-58whwspe	11	16	cells	cell	NNS
cord-326027-58whwspe	11	17	in	in	IN
cord-326027-58whwspe	11	18	real	real	JJ
cord-326027-58whwspe	11	19	time	time	NN
cord-326027-58whwspe	11	20	,	,	,
cord-326027-58whwspe	11	21	we	-PRON-	PRP
cord-326027-58whwspe	11	22	produced	produce	VBD
cord-326027-58whwspe	11	23	an	an	DT
cord-326027-58whwspe	11	24	infectious	infectious	JJ
cord-326027-58whwspe	11	25	recombinant	recombinant	JJ
cord-326027-58whwspe	11	26	ASFV	ASFV	NNP
cord-326027-58whwspe	11	27	(	(	-LRB-
cord-326027-58whwspe	11	28	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	11	29	)	)	-RRB-
cord-326027-58whwspe	11	30	that	that	WDT
cord-326027-58whwspe	11	31	expresses	express	VBZ
cord-326027-58whwspe	11	32	and	and	CC
cord-326027-58whwspe	11	33	incorporates	incorporate	VBZ
cord-326027-58whwspe	11	34	into	into	IN
cord-326027-58whwspe	11	35	the	the	DT
cord-326027-58whwspe	11	36	virus	virus	NN
cord-326027-58whwspe	11	37	particle	particle	NN
cord-326027-58whwspe	11	38	a	a	DT
cord-326027-58whwspe	11	39	chimera	chimera	NN
cord-326027-58whwspe	11	40	of	of	IN
cord-326027-58whwspe	11	41	the	the	DT
cord-326027-58whwspe	11	42	p54	p54	NN
cord-326027-58whwspe	11	43	envelope	envelope	NN
cord-326027-58whwspe	11	44	protein	protein	NN
cord-326027-58whwspe	11	45	fused	fuse	VBN
cord-326027-58whwspe	11	46	to	to	IN
cord-326027-58whwspe	11	47	the	the	DT
cord-326027-58whwspe	11	48	enhanced	enhance	VBN
cord-326027-58whwspe	11	49	green	green	JJ
cord-326027-58whwspe	11	50	fluorescent	fluorescent	NN
cord-326027-58whwspe	11	51	protein	protein	NN
cord-326027-58whwspe	11	52	(	(	-LRB-
cord-326027-58whwspe	11	53	EGFP	EGFP	NNP
cord-326027-58whwspe	11	54	)	)	-RRB-
cord-326027-58whwspe	11	55	.	.	.
cord-326027-58whwspe	12	1	The	the	DT
cord-326027-58whwspe	12	2	incorporation	incorporation	NN
cord-326027-58whwspe	12	3	of	of	IN
cord-326027-58whwspe	12	4	the	the	DT
cord-326027-58whwspe	12	5	fusion	fusion	NN
cord-326027-58whwspe	12	6	protein	protein	NN
cord-326027-58whwspe	12	7	into	into	IN
cord-326027-58whwspe	12	8	the	the	DT
cord-326027-58whwspe	12	9	virus	virus	NN
cord-326027-58whwspe	12	10	particle	particle	NN
cord-326027-58whwspe	12	11	was	be	VBD
cord-326027-58whwspe	12	12	confirmed	confirm	VBN
cord-326027-58whwspe	12	13	immunologically	immunologically	RB
cord-326027-58whwspe	12	14	and	and	CC
cord-326027-58whwspe	12	15	it	-PRON-	PRP
cord-326027-58whwspe	12	16	was	be	VBD
cord-326027-58whwspe	12	17	determined	determine	VBN
cord-326027-58whwspe	12	18	that	that	IN
cord-326027-58whwspe	12	19	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	12	20	was	be	VBD
cord-326027-58whwspe	12	21	fully	fully	RB
cord-326027-58whwspe	12	22	functional	functional	JJ
cord-326027-58whwspe	12	23	by	by	IN
cord-326027-58whwspe	12	24	confirmation	confirmation	NN
cord-326027-58whwspe	12	25	that	that	IN
cord-326027-58whwspe	12	26	the	the	DT
cord-326027-58whwspe	12	27	recombinant	recombinant	JJ
cord-326027-58whwspe	12	28	virus	virus	NN
cord-326027-58whwspe	12	29	made	make	VBD
cord-326027-58whwspe	12	30	normal	normal	JJ
cord-326027-58whwspe	12	31	-	-	HYPH
cord-326027-58whwspe	12	32	sized	sized	JJ
cord-326027-58whwspe	12	33	plaques	plaque	NNS
cord-326027-58whwspe	12	34	and	and	CC
cord-326027-58whwspe	12	35	presented	present	VBD
cord-326027-58whwspe	12	36	similar	similar	JJ
cord-326027-58whwspe	12	37	growth	growth	NN
cord-326027-58whwspe	12	38	curves	curve	NNS
cord-326027-58whwspe	12	39	to	to	IN
cord-326027-58whwspe	12	40	the	the	DT
cord-326027-58whwspe	12	41	wild	wild	JJ
cord-326027-58whwspe	12	42	-	-	HYPH
cord-326027-58whwspe	12	43	type	type	NN
cord-326027-58whwspe	12	44	virus	virus	NN
cord-326027-58whwspe	12	45	.	.	.
cord-326027-58whwspe	13	1	The	the	DT
cord-326027-58whwspe	13	2	tagged	tag	VBN
cord-326027-58whwspe	13	3	virus	virus	NN
cord-326027-58whwspe	13	4	was	be	VBD
cord-326027-58whwspe	13	5	visualized	visualize	VBN
cord-326027-58whwspe	13	6	as	as	IN
cord-326027-58whwspe	13	7	individual	individual	JJ
cord-326027-58whwspe	13	8	fluorescent	fluorescent	NN
cord-326027-58whwspe	13	9	particles	particle	NNS
cord-326027-58whwspe	13	10	during	during	IN
cord-326027-58whwspe	13	11	the	the	DT
cord-326027-58whwspe	13	12	first	first	JJ
cord-326027-58whwspe	13	13	stages	stage	NNS
cord-326027-58whwspe	13	14	of	of	IN
cord-326027-58whwspe	13	15	infection	infection	NN
cord-326027-58whwspe	13	16	and	and	CC
cord-326027-58whwspe	13	17	allowed	allow	VBN
cord-326027-58whwspe	13	18	to	to	TO
cord-326027-58whwspe	13	19	visualize	visualize	VB
cord-326027-58whwspe	13	20	the	the	DT
cord-326027-58whwspe	13	21	infection	infection	NN
cord-326027-58whwspe	13	22	progression	progression	NN
cord-326027-58whwspe	13	23	in	in	IN
cord-326027-58whwspe	13	24	living	live	VBG
cord-326027-58whwspe	13	25	cells	cell	NNS
cord-326027-58whwspe	13	26	through	through	IN
cord-326027-58whwspe	13	27	the	the	DT
cord-326027-58whwspe	13	28	viral	viral	JJ
cord-326027-58whwspe	13	29	life	life	NN
cord-326027-58whwspe	13	30	cycle	cycle	NN
cord-326027-58whwspe	13	31	by	by	IN
cord-326027-58whwspe	13	32	confocal	confocal	JJ
cord-326027-58whwspe	13	33	microscopy	microscopy	NNS
cord-326027-58whwspe	13	34	.	.	.
cord-326027-58whwspe	14	1	In	in	IN
cord-326027-58whwspe	14	2	this	this	DT
cord-326027-58whwspe	14	3	work	work	NN
cord-326027-58whwspe	14	4	,	,	,
cord-326027-58whwspe	14	5	diverse	diverse	JJ
cord-326027-58whwspe	14	6	potential	potential	JJ
cord-326027-58whwspe	14	7	applications	application	NNS
cord-326027-58whwspe	14	8	of	of	IN
cord-326027-58whwspe	14	9	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	14	10	to	to	TO
cord-326027-58whwspe	14	11	study	study	VB
cord-326027-58whwspe	14	12	different	different	JJ
cord-326027-58whwspe	14	13	aspects	aspect	NNS
cord-326027-58whwspe	14	14	of	of	IN
cord-326027-58whwspe	14	15	ASFV	ASFV	NNP
cord-326027-58whwspe	14	16	infection	infection	NN
cord-326027-58whwspe	14	17	are	be	VBP
cord-326027-58whwspe	14	18	shown	show	VBN
cord-326027-58whwspe	14	19	.	.	.
cord-326027-58whwspe	15	1	By	by	IN
cord-326027-58whwspe	15	2	using	use	VBG
cord-326027-58whwspe	15	3	this	this	DT
cord-326027-58whwspe	15	4	recombinant	recombinant	JJ
cord-326027-58whwspe	15	5	virus	virus	NN
cord-326027-58whwspe	15	6	it	-PRON-	PRP
cord-326027-58whwspe	15	7	was	be	VBD
cord-326027-58whwspe	15	8	possible	possible	JJ
cord-326027-58whwspe	15	9	to	to	TO
cord-326027-58whwspe	15	10	determine	determine	VB
cord-326027-58whwspe	15	11	the	the	DT
cord-326027-58whwspe	15	12	trajectory	trajectory	NN
cord-326027-58whwspe	15	13	and	and	CC
cord-326027-58whwspe	15	14	speed	speed	NN
cord-326027-58whwspe	15	15	of	of	IN
cord-326027-58whwspe	15	16	intracellular	intracellular	JJ
cord-326027-58whwspe	15	17	virus	virus	NN
cord-326027-58whwspe	15	18	movement	movement	NN
cord-326027-58whwspe	15	19	.	.	.
cord-326027-58whwspe	16	1	Additionally	additionally	RB
cord-326027-58whwspe	16	2	,	,	,
cord-326027-58whwspe	16	3	we	-PRON-	PRP
cord-326027-58whwspe	16	4	have	have	VBP
cord-326027-58whwspe	16	5	been	be	VBN
cord-326027-58whwspe	16	6	able	able	JJ
cord-326027-58whwspe	16	7	to	to	TO
cord-326027-58whwspe	16	8	visualize	visualize	VB
cord-326027-58whwspe	16	9	for	for	IN
cord-326027-58whwspe	16	10	first	first	JJ
cord-326027-58whwspe	16	11	time	time	NN
cord-326027-58whwspe	16	12	the	the	DT
cord-326027-58whwspe	16	13	ASFV	ASFV	NNP
cord-326027-58whwspe	16	14	factory	factory	NN
cord-326027-58whwspe	16	15	formation	formation	NN
cord-326027-58whwspe	16	16	dynamics	dynamic	NNS
cord-326027-58whwspe	16	17	and	and	CC
cord-326027-58whwspe	16	18	the	the	DT
cord-326027-58whwspe	16	19	cytophatic	cytophatic	JJ
cord-326027-58whwspe	16	20	effect	effect	NN
cord-326027-58whwspe	16	21	of	of	IN
cord-326027-58whwspe	16	22	the	the	DT
cord-326027-58whwspe	16	23	virus	virus	NN
cord-326027-58whwspe	16	24	in	in	IN
cord-326027-58whwspe	16	25	live	live	JJ
cord-326027-58whwspe	16	26	infected	infected	JJ
cord-326027-58whwspe	16	27	cells	cell	NNS
cord-326027-58whwspe	16	28	.	.	.
cord-326027-58whwspe	17	1	Finally	finally	RB
cord-326027-58whwspe	17	2	,	,	,
cord-326027-58whwspe	17	3	we	-PRON-	PRP
cord-326027-58whwspe	17	4	have	have	VBP
cord-326027-58whwspe	17	5	analyzed	analyze	VBN
cord-326027-58whwspe	17	6	virus	virus	NN
cord-326027-58whwspe	17	7	progression	progression	NN
cord-326027-58whwspe	17	8	along	along	IN
cord-326027-58whwspe	17	9	the	the	DT
cord-326027-58whwspe	17	10	infection	infection	NN
cord-326027-58whwspe	17	11	cycle	cycle	NN
cord-326027-58whwspe	17	12	and	and	CC
cord-326027-58whwspe	17	13	infected	infected	JJ
cord-326027-58whwspe	17	14	cell	cell	NN
cord-326027-58whwspe	17	15	death	death	NN
cord-326027-58whwspe	17	16	as	as	IN
cord-326027-58whwspe	17	17	time	time	NN
cord-326027-58whwspe	17	18	-	-	HYPH
cord-326027-58whwspe	17	19	lapse	lapse	NN
cord-326027-58whwspe	17	20	animations	animation	NNS
cord-326027-58whwspe	17	21	.	.	.
cord-326027-58whwspe	18	1	Many	many	JJ
cord-326027-58whwspe	18	2	aspects	aspect	NNS
cord-326027-58whwspe	18	3	of	of	IN
cord-326027-58whwspe	18	4	the	the	DT
cord-326027-58whwspe	18	5	infection	infection	NN
cord-326027-58whwspe	18	6	cycle	cycle	NN
cord-326027-58whwspe	18	7	of	of	IN
cord-326027-58whwspe	18	8	African	african	JJ
cord-326027-58whwspe	18	9	swine	swine	NN
cord-326027-58whwspe	18	10	fever	fever	NN
cord-326027-58whwspe	18	11	virus	virus	NN
cord-326027-58whwspe	18	12	(	(	-LRB-
cord-326027-58whwspe	18	13	ASFV	ASFV	NNP
cord-326027-58whwspe	18	14	)	)	-RRB-
cord-326027-58whwspe	18	15	remain	remain	VBP
cord-326027-58whwspe	18	16	still	still	RB
cord-326027-58whwspe	18	17	poorly	poorly	RB
cord-326027-58whwspe	18	18	understood	understand	VBN
cord-326027-58whwspe	18	19	.	.	.
cord-326027-58whwspe	19	1	Some	some	DT
cord-326027-58whwspe	19	2	events	event	NNS
cord-326027-58whwspe	19	3	of	of	IN
cord-326027-58whwspe	19	4	the	the	DT
cord-326027-58whwspe	19	5	infection	infection	NN
cord-326027-58whwspe	19	6	such	such	PDT
cord-326027-58whwspe	19	7	those	those	DT
cord-326027-58whwspe	19	8	viral	viral	JJ
cord-326027-58whwspe	19	9	proteins	protein	NNS
cord-326027-58whwspe	19	10	involved	involve	VBN
cord-326027-58whwspe	19	11	in	in	IN
cord-326027-58whwspe	19	12	the	the	DT
cord-326027-58whwspe	19	13	virus	virus	NN
cord-326027-58whwspe	19	14	attachment	attachment	NN
cord-326027-58whwspe	19	15	,	,	,
cord-326027-58whwspe	19	16	the	the	DT
cord-326027-58whwspe	19	17	intracellular	intracellular	JJ
cord-326027-58whwspe	19	18	transport	transport	NN
cord-326027-58whwspe	19	19	to	to	IN
cord-326027-58whwspe	19	20	perinuclear	perinuclear	NN
cord-326027-58whwspe	19	21	areas	area	NNS
cord-326027-58whwspe	19	22	of	of	IN
cord-326027-58whwspe	19	23	the	the	DT
cord-326027-58whwspe	19	24	nucleus	nucleus	NN
cord-326027-58whwspe	19	25	for	for	IN
cord-326027-58whwspe	19	26	virus	virus	NN
cord-326027-58whwspe	19	27	replication	replication	NN
cord-326027-58whwspe	19	28	,	,	,
cord-326027-58whwspe	19	29	as	as	RB
cord-326027-58whwspe	19	30	well	well	RB
cord-326027-58whwspe	19	31	as	as	IN
cord-326027-58whwspe	19	32	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	19	33	and	and	CC
cord-326027-58whwspe	19	34	transport	transport	NN
cord-326027-58whwspe	19	35	events	event	NNS
cord-326027-58whwspe	19	36	of	of	IN
cord-326027-58whwspe	19	37	the	the	DT
cord-326027-58whwspe	19	38	intracellular	intracellular	JJ
cord-326027-58whwspe	19	39	virus	virus	NN
cord-326027-58whwspe	19	40	away	away	RB
cord-326027-58whwspe	19	41	from	from	IN
cord-326027-58whwspe	19	42	the	the	DT
cord-326027-58whwspe	19	43	factories	factory	NNS
cord-326027-58whwspe	19	44	have	have	VBP
cord-326027-58whwspe	19	45	been	be	VBN
cord-326027-58whwspe	19	46	biochemically	biochemically	RB
cord-326027-58whwspe	19	47	defined	define	VBN
cord-326027-58whwspe	19	48	.	.	.
cord-326027-58whwspe	20	1	However	however	RB
cord-326027-58whwspe	20	2	,	,	,
cord-326027-58whwspe	20	3	a	a	DT
cord-326027-58whwspe	20	4	clear	clear	JJ
cord-326027-58whwspe	20	5	understanding	understanding	NN
cord-326027-58whwspe	20	6	of	of	IN
cord-326027-58whwspe	20	7	these	these	DT
cord-326027-58whwspe	20	8	processes	process	NNS
cord-326027-58whwspe	20	9	,	,	,
cord-326027-58whwspe	20	10	how	how	WRB
cord-326027-58whwspe	20	11	and	and	CC
cord-326027-58whwspe	20	12	when	when	WRB
cord-326027-58whwspe	20	13	they	-PRON-	PRP
cord-326027-58whwspe	20	14	occur	occur	VBP
cord-326027-58whwspe	20	15	,	,	,
cord-326027-58whwspe	20	16	has	have	VBZ
cord-326027-58whwspe	20	17	been	be	VBN
cord-326027-58whwspe	20	18	hampered	hamper	VBN
cord-326027-58whwspe	20	19	by	by	IN
cord-326027-58whwspe	20	20	the	the	DT
cord-326027-58whwspe	20	21	inability	inability	NN
cord-326027-58whwspe	20	22	to	to	TO
cord-326027-58whwspe	20	23	directly	directly	RB
cord-326027-58whwspe	20	24	observe	observe	VB
cord-326027-58whwspe	20	25	these	these	DT
cord-326027-58whwspe	20	26	events	event	NNS
cord-326027-58whwspe	20	27	in	in	IN
cord-326027-58whwspe	20	28	infected	infected	JJ
cord-326027-58whwspe	20	29	cells	cell	NNS
cord-326027-58whwspe	20	30	.	.	.
cord-326027-58whwspe	21	1	ASFV	ASFV	NNP
cord-326027-58whwspe	21	2	assembly	assembly	NN
cord-326027-58whwspe	21	3	occurs	occur	VBZ
cord-326027-58whwspe	21	4	in	in	IN
cord-326027-58whwspe	21	5	viral	viral	JJ
cord-326027-58whwspe	21	6	factories	factory	NNS
cord-326027-58whwspe	21	7	that	that	WDT
cord-326027-58whwspe	21	8	contain	contain	VBP
cord-326027-58whwspe	21	9	high	high	JJ
cord-326027-58whwspe	21	10	levels	level	NNS
cord-326027-58whwspe	21	11	of	of	IN
cord-326027-58whwspe	21	12	viral	viral	JJ
cord-326027-58whwspe	21	13	structural	structural	JJ
cord-326027-58whwspe	21	14	proteins	protein	NNS
cord-326027-58whwspe	21	15	,	,	,
cord-326027-58whwspe	21	16	viral	viral	JJ
cord-326027-58whwspe	21	17	DNA	dna	NN
cord-326027-58whwspe	21	18	,	,	,
cord-326027-58whwspe	21	19	and	and	CC
cord-326027-58whwspe	21	20	amorphous	amorphous	JJ
cord-326027-58whwspe	21	21	membranous	membranous	JJ
cord-326027-58whwspe	21	22	material	material	NN
cord-326027-58whwspe	21	23	used	use	VBN
cord-326027-58whwspe	21	24	to	to	TO
cord-326027-58whwspe	21	25	produce	produce	VB
cord-326027-58whwspe	21	26	viral	viral	JJ
cord-326027-58whwspe	21	27	envelopes	envelope	NNS
cord-326027-58whwspe	21	28	.	.	.
cord-326027-58whwspe	22	1	The	the	DT
cord-326027-58whwspe	22	2	170-kb	170-kb	CD
cord-326027-58whwspe	22	3	genome	genome	NN
cord-326027-58whwspe	22	4	of	of	IN
cord-326027-58whwspe	22	5	ASFV	ASFV	NNP
cord-326027-58whwspe	22	6	encodes	encode	VBZ
cord-326027-58whwspe	22	7	some	some	DT
cord-326027-58whwspe	22	8	150	150	CD
cord-326027-58whwspe	22	9	open	open	JJ
cord-326027-58whwspe	22	10	reading	reading	NN
cord-326027-58whwspe	22	11	frames	frame	NNS
cord-326027-58whwspe	22	12	,	,	,
cord-326027-58whwspe	22	13	and	and	CC
cord-326027-58whwspe	22	14	as	as	RB
cord-326027-58whwspe	22	15	many	many	JJ
cord-326027-58whwspe	22	16	as	as	IN
cord-326027-58whwspe	22	17	50	50	CD
cord-326027-58whwspe	22	18	viral	viral	JJ
cord-326027-58whwspe	22	19	proteins	protein	NNS
cord-326027-58whwspe	22	20	are	be	VBP
cord-326027-58whwspe	22	21	incorporated	incorporate	VBN
cord-326027-58whwspe	22	22	to	to	IN
cord-326027-58whwspe	22	23	the	the	DT
cord-326027-58whwspe	22	24	viral	viral	JJ
cord-326027-58whwspe	22	25	particle	particle	NN
cord-326027-58whwspe	22	26	(	(	-LRB-
cord-326027-58whwspe	22	27	Esteves	Esteves	NNP
cord-326027-58whwspe	22	28	et	et	NNP
cord-326027-58whwspe	22	29	al	al	NNP
cord-326027-58whwspe	22	30	.	.	NNP
cord-326027-58whwspe	22	31	,	,	,
cord-326027-58whwspe	22	32	1986	1986	CD
cord-326027-58whwspe	22	33	)	)	-RRB-
cord-326027-58whwspe	22	34	.	.	.
cord-326027-58whwspe	23	1	Approximately	approximately	RB
cord-326027-58whwspe	23	2	35	35	CD
cord-326027-58whwspe	23	3	%	%	NN
cord-326027-58whwspe	23	4	of	of	IN
cord-326027-58whwspe	23	5	the	the	DT
cord-326027-58whwspe	23	6	mass	mass	NN
cord-326027-58whwspe	23	7	of	of	IN
cord-326027-58whwspe	23	8	the	the	DT
cord-326027-58whwspe	23	9	virion	virion	NN
cord-326027-58whwspe	23	10	is	be	VBZ
cord-326027-58whwspe	23	11	provided	provide	VBN
cord-326027-58whwspe	23	12	by	by	IN
cord-326027-58whwspe	23	13	p72	p72	NN
cord-326027-58whwspe	23	14	,	,	,
cord-326027-58whwspe	23	15	the	the	DT
cord-326027-58whwspe	23	16	major	major	JJ
cord-326027-58whwspe	23	17	capsid	capsid	NN
cord-326027-58whwspe	23	18	protein	protein	NN
cord-326027-58whwspe	23	19	,	,	,
cord-326027-58whwspe	23	20	while	while	IN
cord-326027-58whwspe	23	21	the	the	DT
cord-326027-58whwspe	23	22	structural	structural	JJ
cord-326027-58whwspe	23	23	proteins	protein	NNS
cord-326027-58whwspe	23	24	p150	p150	VBG
cord-326027-58whwspe	23	25	,	,	,
cord-326027-58whwspe	23	26	p37	p37	NNP
cord-326027-58whwspe	23	27	,	,	,
cord-326027-58whwspe	23	28	p34	p34	CD
cord-326027-58whwspe	23	29	and	and	CC
cord-326027-58whwspe	23	30	p14	p14	NN
cord-326027-58whwspe	23	31	,	,	,
cord-326027-58whwspe	23	32	all	all	DT
cord-326027-58whwspe	23	33	of	of	IN
cord-326027-58whwspe	23	34	them	-PRON-	PRP
cord-326027-58whwspe	23	35	derived	derive	VBD
cord-326027-58whwspe	23	36	from	from	IN
cord-326027-58whwspe	23	37	polyprotein	polyprotein	NNP
cord-326027-58whwspe	23	38	p220	p220	NNP
cord-326027-58whwspe	23	39	,	,	,
cord-326027-58whwspe	23	40	provides	provide	VBZ
cord-326027-58whwspe	23	41	another	another	DT
cord-326027-58whwspe	23	42	25	25	CD
cord-326027-58whwspe	23	43	%	%	NN
cord-326027-58whwspe	23	44	of	of	IN
cord-326027-58whwspe	23	45	the	the	DT
cord-326027-58whwspe	23	46	virion	virion	NN
cord-326027-58whwspe	23	47	mass	mass	NN
cord-326027-58whwspe	23	48	(	(	-LRB-
cord-326027-58whwspe	23	49	Andres	Andres	NNP
cord-326027-58whwspe	23	50	et	et	NNP
cord-326027-58whwspe	23	51	al	al	NNP
cord-326027-58whwspe	23	52	.	.	NNP
cord-326027-58whwspe	23	53	,	,	,
cord-326027-58whwspe	23	54	1997	1997	CD
cord-326027-58whwspe	23	55	)	)	-RRB-
cord-326027-58whwspe	23	56	.	.	.
cord-326027-58whwspe	24	1	ASFV	ASFV	NNP
cord-326027-58whwspe	24	2	particles	particle	NNS
cord-326027-58whwspe	24	3	assemble	assemble	VBP
cord-326027-58whwspe	24	4	within	within	IN
cord-326027-58whwspe	24	5	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	24	6	viral	viral	JJ
cord-326027-58whwspe	24	7	factories	factory	NNS
cord-326027-58whwspe	24	8	from	from	IN
cord-326027-58whwspe	24	9	endoplasmic	endoplasmic	NN
cord-326027-58whwspe	24	10	reticulum	reticulum	NN
cord-326027-58whwspe	24	11	-	-	HYPH
cord-326027-58whwspe	24	12	derived	derive	VBN
cord-326027-58whwspe	24	13	viral	viral	JJ
cord-326027-58whwspe	24	14	membranes	membrane	NNS
cord-326027-58whwspe	24	15	(	(	-LRB-
cord-326027-58whwspe	25	1	Andres	Andres	NNP
cord-326027-58whwspe	25	2	et	et	NNP
cord-326027-58whwspe	25	3	al	al	NNP
cord-326027-58whwspe	25	4	.	.	.
cord-326027-58whwspe	26	1	,	,	,
cord-326027-58whwspe	26	2	1997	1997	CD
cord-326027-58whwspe	27	1	Rouiller	Rouiller	NNP
cord-326027-58whwspe	27	2	et	et	NNP
cord-326027-58whwspe	27	3	al	al	NNP
cord-326027-58whwspe	27	4	.	.	NNP
cord-326027-58whwspe	27	5	,	,	,
cord-326027-58whwspe	27	6	1998	1998	CD
cord-326027-58whwspe	27	7	)	)	-RRB-
cord-326027-58whwspe	27	8	at	at	IN
cord-326027-58whwspe	27	9	perinuclear	perinuclear	NNP
cord-326027-58whwspe	27	10	sites	sites	NNPS
cord-326027-58whwspe	28	1	(	(	-LRB-
cord-326027-58whwspe	28	2	Nunes	Nunes	NNP
cord-326027-58whwspe	28	3	et	et	NNP
cord-326027-58whwspe	28	4	al	al	NNP
cord-326027-58whwspe	28	5	.	.	NNP
cord-326027-58whwspe	28	6	,	,	,
cord-326027-58whwspe	28	7	1975	1975	CD
cord-326027-58whwspe	28	8	)	)	-RRB-
cord-326027-58whwspe	28	9	that	that	WDT
cord-326027-58whwspe	28	10	contain	contain	VBP
cord-326027-58whwspe	28	11	fully	fully	RB
cord-326027-58whwspe	28	12	assembled	assemble	VBN
cord-326027-58whwspe	28	13	virions	virion	NNS
cord-326027-58whwspe	28	14	seen	see	VBN
cord-326027-58whwspe	28	15	as	as	IN
cord-326027-58whwspe	28	16	200-nm	200-nm	CD
cord-326027-58whwspe	28	17	-	-	HYPH
cord-326027-58whwspe	28	18	diameter	diameter	NN
cord-326027-58whwspe	28	19	hexagons	hexagon	NNS
cord-326027-58whwspe	28	20	in	in	IN
cord-326027-58whwspe	28	21	cross	cross	NN
cord-326027-58whwspe	28	22	-	-	NN
cord-326027-58whwspe	28	23	section	section	NN
cord-326027-58whwspe	28	24	,	,	,
cord-326027-58whwspe	28	25	and	and	CC
cord-326027-58whwspe	28	26	a	a	DT
cord-326027-58whwspe	28	27	series	series	NN
cord-326027-58whwspe	28	28	of	of	IN
cord-326027-58whwspe	28	29	one	one	CD
cord-326027-58whwspe	28	30	to	to	TO
cord-326027-58whwspe	28	31	six	six	CD
cord-326027-58whwspe	28	32	-	-	HYPH
cord-326027-58whwspe	28	33	sided	sided	JJ
cord-326027-58whwspe	28	34	assembly	assembly	NN
cord-326027-58whwspe	28	35	intermediates	intermediate	NNS
cord-326027-58whwspe	28	36	(	(	-LRB-
cord-326027-58whwspe	28	37	Rouiller	Rouiller	NNP
cord-326027-58whwspe	28	38	et	et	NNP
cord-326027-58whwspe	28	39	al	al	NNP
cord-326027-58whwspe	28	40	.	.	NNP
cord-326027-58whwspe	28	41	,	,	,
cord-326027-58whwspe	28	42	1998	1998	CD
cord-326027-58whwspe	28	43	)	)	-RRB-
cord-326027-58whwspe	28	44	.	.	.
cord-326027-58whwspe	29	1	Morphological	morphological	JJ
cord-326027-58whwspe	29	2	evidence	evidence	NN
cord-326027-58whwspe	29	3	indicates	indicate	VBZ
cord-326027-58whwspe	29	4	that	that	IN
cord-326027-58whwspe	29	5	viral	viral	JJ
cord-326027-58whwspe	29	6	membranes	membrane	NNS
cord-326027-58whwspe	29	7	become	become	VBP
cord-326027-58whwspe	29	8	icosahedral	icosahedral	JJ
cord-326027-58whwspe	29	9	particles	particle	NNS
cord-326027-58whwspe	29	10	by	by	IN
cord-326027-58whwspe	29	11	the	the	DT
cord-326027-58whwspe	29	12	progressive	progressive	JJ
cord-326027-58whwspe	29	13	construction	construction	NN
cord-326027-58whwspe	29	14	of	of	IN
cord-326027-58whwspe	29	15	the	the	DT
cord-326027-58whwspe	29	16	outer	outer	JJ
cord-326027-58whwspe	29	17	capsid	capsid	NN
cord-326027-58whwspe	29	18	layer	layer	NN
cord-326027-58whwspe	29	19	,	,	,
cord-326027-58whwspe	29	20	which	which	WDT
cord-326027-58whwspe	29	21	is	be	VBZ
cord-326027-58whwspe	29	22	composed	compose	VBN
cord-326027-58whwspe	29	23	mainly	mainly	RB
cord-326027-58whwspe	29	24	of	of	IN
cord-326027-58whwspe	29	25	viral	viral	JJ
cord-326027-58whwspe	29	26	protein	protein	NN
cord-326027-58whwspe	29	27	p72	p72	NN
cord-326027-58whwspe	29	28	.	.	.
cord-326027-58whwspe	30	1	Biochemical	biochemical	JJ
cord-326027-58whwspe	30	2	data	datum	NNS
cord-326027-58whwspe	30	3	also	also	RB
cord-326027-58whwspe	30	4	suggest	suggest	VBP
cord-326027-58whwspe	30	5	that	that	IN
cord-326027-58whwspe	30	6	protein	protein	NN
cord-326027-58whwspe	30	7	p72	p72	NN
cord-326027-58whwspe	30	8	is	be	VBZ
cord-326027-58whwspe	30	9	assembled	assemble	VBN
cord-326027-58whwspe	30	10	in	in	IN
cord-326027-58whwspe	30	11	a	a	DT
cord-326027-58whwspe	30	12	time	time	NN
cord-326027-58whwspe	30	13	-	-	HYPH
cord-326027-58whwspe	30	14	dependent	dependent	JJ
cord-326027-58whwspe	30	15	fashion	fashion	NN
cord-326027-58whwspe	30	16	into	into	IN
cord-326027-58whwspe	30	17	large	large	JJ
cord-326027-58whwspe	30	18	membrane	membrane	NN
cord-326027-58whwspe	30	19	-	-	HYPH
cord-326027-58whwspe	30	20	bound	bind	VBN
cord-326027-58whwspe	30	21	complexes	complex	NNS
cord-326027-58whwspe	30	22	that	that	WDT
cord-326027-58whwspe	30	23	may	may	MD
cord-326027-58whwspe	30	24	correspond	correspond	VB
cord-326027-58whwspe	30	25	to	to	IN
cord-326027-58whwspe	30	26	capsid	capsid	JJ
cord-326027-58whwspe	30	27	-	-	HYPH
cord-326027-58whwspe	30	28	like	like	JJ
cord-326027-58whwspe	30	29	structures	structure	NNS
cord-326027-58whwspe	30	30	(	(	-LRB-
cord-326027-58whwspe	30	31	Cobbold	Cobbold	NNP
cord-326027-58whwspe	30	32	and	and	CC
cord-326027-58whwspe	30	33	Wileman	Wileman	NNP
cord-326027-58whwspe	30	34	,	,	,
cord-326027-58whwspe	30	35	1998	1998	CD
cord-326027-58whwspe	30	36	)	)	-RRB-
cord-326027-58whwspe	30	37	.	.	.
cord-326027-58whwspe	31	1	Concomitantly	concomitantly	RB
cord-326027-58whwspe	31	2	,	,	,
cord-326027-58whwspe	31	3	the	the	DT
cord-326027-58whwspe	31	4	core	core	NN
cord-326027-58whwspe	31	5	shell	shell	NN
cord-326027-58whwspe	31	6	is	be	VBZ
cord-326027-58whwspe	31	7	formed	form	VBN
cord-326027-58whwspe	31	8	underneath	underneath	IN
cord-326027-58whwspe	31	9	the	the	DT
cord-326027-58whwspe	31	10	viral	viral	JJ
cord-326027-58whwspe	31	11	envelope	envelope	NN
cord-326027-58whwspe	31	12	,	,	,
cord-326027-58whwspe	31	13	and	and	CC
cord-326027-58whwspe	31	14	subsequently	subsequently	RB
cord-326027-58whwspe	31	15	the	the	DT
cord-326027-58whwspe	31	16	viral	viral	JJ
cord-326027-58whwspe	31	17	DNA	dna	NN
cord-326027-58whwspe	31	18	and	and	CC
cord-326027-58whwspe	31	19	nucleoproteins	nucleoprotein	NNS
cord-326027-58whwspe	31	20	are	be	VBP
cord-326027-58whwspe	31	21	packaged	package	VBN
cord-326027-58whwspe	31	22	and	and	CC
cord-326027-58whwspe	31	23	condensed	condense	VBN
cord-326027-58whwspe	31	24	to	to	TO
cord-326027-58whwspe	31	25	form	form	VB
cord-326027-58whwspe	31	26	the	the	DT
cord-326027-58whwspe	31	27	electrondense	electrondense	NNP
cord-326027-58whwspe	31	28	nucleoid	nucleoid	NNP
cord-326027-58whwspe	31	29	(	(	-LRB-
cord-326027-58whwspe	31	30	Andres	Andres	NNP
cord-326027-58whwspe	31	31	et	et	NNP
cord-326027-58whwspe	31	32	al	al	NNP
cord-326027-58whwspe	31	33	.	.	NNP
cord-326027-58whwspe	31	34	,	,	,
cord-326027-58whwspe	31	35	1997	1997	CD
cord-326027-58whwspe	32	1	(	(	-LRB-
cord-326027-58whwspe	32	2	Andres	Andres	NNP
cord-326027-58whwspe	32	3	et	et	NNP
cord-326027-58whwspe	32	4	al	al	NNP
cord-326027-58whwspe	32	5	.	.	.
cord-326027-58whwspe	33	1	,	,	,
cord-326027-58whwspe	33	2	,	,	,
cord-326027-58whwspe	33	3	2002	2002	CD
cord-326027-58whwspe	33	4	Brookes	Brookes	NNP
cord-326027-58whwspe	33	5	et	et	NNP
cord-326027-58whwspe	33	6	al	al	NNP
cord-326027-58whwspe	33	7	.	.	NNP
cord-326027-58whwspe	33	8	,	,	,
cord-326027-58whwspe	33	9	1998	1998	CD
cord-326027-58whwspe	33	10	)	)	-RRB-
cord-326027-58whwspe	33	11	.	.	.
cord-326027-58whwspe	34	1	Intracellular	Intracellular	NNP
cord-326027-58whwspe	34	2	mature	mature	JJ
cord-326027-58whwspe	34	3	virions	virion	NNS
cord-326027-58whwspe	34	4	made	make	VBN
cord-326027-58whwspe	34	5	at	at	IN
cord-326027-58whwspe	34	6	the	the	DT
cord-326027-58whwspe	34	7	assembly	assembly	NN
cord-326027-58whwspe	34	8	sites	site	NNS
cord-326027-58whwspe	34	9	are	be	VBP
cord-326027-58whwspe	34	10	infectious	infectious	JJ
cord-326027-58whwspe	35	1	(	(	-LRB-
cord-326027-58whwspe	35	2	Andres	Andres	NNP
cord-326027-58whwspe	35	3	et	et	NNP
cord-326027-58whwspe	35	4	al	al	NNP
cord-326027-58whwspe	35	5	.	.	NNP
cord-326027-58whwspe	35	6	,	,	,
cord-326027-58whwspe	35	7	2001	2001	CD
cord-326027-58whwspe	35	8	)	)	-RRB-
cord-326027-58whwspe	35	9	.	.	.
cord-326027-58whwspe	36	1	A	a	DT
cord-326027-58whwspe	36	2	fraction	fraction	NN
cord-326027-58whwspe	36	3	of	of	IN
cord-326027-58whwspe	36	4	them	-PRON-	PRP
cord-326027-58whwspe	36	5	,	,	,
cord-326027-58whwspe	36	6	however	however	RB
cord-326027-58whwspe	36	7	are	be	VBP
cord-326027-58whwspe	36	8	transported	transport	VBN
cord-326027-58whwspe	36	9	by	by	IN
cord-326027-58whwspe	36	10	microtubule	microtubule	NN
cord-326027-58whwspe	36	11	-	-	HYPH
cord-326027-58whwspe	36	12	mediated	mediate	VBN
cord-326027-58whwspe	36	13	transport	transport	NN
cord-326027-58whwspe	36	14	(	(	-LRB-
cord-326027-58whwspe	36	15	Alonso	Alonso	NNP
cord-326027-58whwspe	36	16	et	et	NNP
cord-326027-58whwspe	36	17	al	al	NNP
cord-326027-58whwspe	36	18	.	.	NNP
cord-326027-58whwspe	36	19	,	,	,
cord-326027-58whwspe	36	20	2001	2001	CD
cord-326027-58whwspe	36	21	;	;	:
cord-326027-58whwspe	36	22	de	de	NNP
cord-326027-58whwspe	36	23	Matos	Matos	NNP
cord-326027-58whwspe	36	24	and	and	CC
cord-326027-58whwspe	36	25	Carvalho	Carvalho	NNP
cord-326027-58whwspe	36	26	,	,	,
cord-326027-58whwspe	36	27	1993	1993	CD
cord-326027-58whwspe	36	28	)	)	-RRB-
cord-326027-58whwspe	36	29	to	to	IN
cord-326027-58whwspe	36	30	the	the	DT
cord-326027-58whwspe	36	31	plasma	plasma	NN
cord-326027-58whwspe	36	32	membrane	membrane	NN
cord-326027-58whwspe	36	33	,	,	,
cord-326027-58whwspe	36	34	where	where	WRB
cord-326027-58whwspe	36	35	they	-PRON-	PRP
cord-326027-58whwspe	36	36	are	be	VBP
cord-326027-58whwspe	36	37	released	release	VBN
cord-326027-58whwspe	36	38	by	by	IN
cord-326027-58whwspe	36	39	budding	bud	VBG
cord-326027-58whwspe	36	40	(	(	-LRB-
cord-326027-58whwspe	36	41	Breese	Breese	NNP
cord-326027-58whwspe	36	42	and	and	CC
cord-326027-58whwspe	36	43	Pan	Pan	NNP
cord-326027-58whwspe	36	44	,	,	,
cord-326027-58whwspe	36	45	1978	1978	CD
cord-326027-58whwspe	36	46	)	)	-RRB-
cord-326027-58whwspe	36	47	to	to	TO
cord-326027-58whwspe	36	48	give	give	VB
cord-326027-58whwspe	36	49	rise	rise	NN
cord-326027-58whwspe	36	50	to	to	IN
cord-326027-58whwspe	36	51	the	the	DT
cord-326027-58whwspe	36	52	infectious	infectious	JJ
cord-326027-58whwspe	36	53	extracellular	extracellular	JJ
cord-326027-58whwspe	36	54	enveloped	envelop	VBN
cord-326027-58whwspe	36	55	virions	virion	NNS
cord-326027-58whwspe	36	56	.	.	.
cord-326027-58whwspe	37	1	Because	because	IN
cord-326027-58whwspe	37	2	of	of	IN
cord-326027-58whwspe	37	3	the	the	DT
cord-326027-58whwspe	37	4	high	high	JJ
cord-326027-58whwspe	37	5	viscosity	viscosity	NN
cord-326027-58whwspe	37	6	of	of	IN
cord-326027-58whwspe	37	7	the	the	DT
cord-326027-58whwspe	37	8	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	37	9	,	,	,
cord-326027-58whwspe	37	10	movement	movement	NN
cord-326027-58whwspe	37	11	of	of	IN
cord-326027-58whwspe	37	12	ASFV	ASFV	NNP
cord-326027-58whwspe	37	13	particles	particle	NNS
cord-326027-58whwspe	37	14	by	by	IN
cord-326027-58whwspe	37	15	diffusion	diffusion	NN
cord-326027-58whwspe	37	16	is	be	VBZ
cord-326027-58whwspe	37	17	likely	likely	JJ
cord-326027-58whwspe	37	18	to	to	TO
cord-326027-58whwspe	37	19	be	be	VB
cord-326027-58whwspe	37	20	very	very	RB
cord-326027-58whwspe	37	21	limited	limited	JJ
cord-326027-58whwspe	37	22	(	(	-LRB-
cord-326027-58whwspe	37	23	for	for	IN
cord-326027-58whwspe	37	24	review	review	NN
cord-326027-58whwspe	37	25	see	see	VB
cord-326027-58whwspe	37	26	(	(	-LRB-
cord-326027-58whwspe	37	27	Luby	Luby	NNP
cord-326027-58whwspe	37	28	-	-	HYPH
cord-326027-58whwspe	37	29	Phelps	Phelps	NNP
cord-326027-58whwspe	37	30	,	,	,
cord-326027-58whwspe	37	31	2000	2000	CD
cord-326027-58whwspe	37	32	)	)	-RRB-
cord-326027-58whwspe	37	33	.	.	.
cord-326027-58whwspe	38	1	Some	some	DT
cord-326027-58whwspe	38	2	viruses	virus	NNS
cord-326027-58whwspe	38	3	overcome	overcome	VBP
cord-326027-58whwspe	38	4	this	this	DT
cord-326027-58whwspe	38	5	obstacle	obstacle	NN
cord-326027-58whwspe	38	6	by	by	IN
cord-326027-58whwspe	38	7	hijacking	hijack	VBG
cord-326027-58whwspe	38	8	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	38	9	motors	motor	NNS
cord-326027-58whwspe	38	10	to	to	TO
cord-326027-58whwspe	38	11	utilize	utilize	VB
cord-326027-58whwspe	38	12	the	the	DT
cord-326027-58whwspe	38	13	cellular	cellular	JJ
cord-326027-58whwspe	38	14	cytoskeleton	cytoskeleton	NN
cord-326027-58whwspe	38	15	as	as	IN
cord-326027-58whwspe	38	16	a	a	DT
cord-326027-58whwspe	38	17	roadway	roadway	NN
cord-326027-58whwspe	38	18	.	.	.
cord-326027-58whwspe	39	1	For	for	IN
cord-326027-58whwspe	39	2	instance	instance	NN
cord-326027-58whwspe	39	3	,	,	,
cord-326027-58whwspe	39	4	herpes	herpes	NN
cord-326027-58whwspe	39	5	simplex	simplex	NN
cord-326027-58whwspe	39	6	virus	virus	NN
cord-326027-58whwspe	39	7	HSV-1	hsv-1	NN
cord-326027-58whwspe	39	8	,	,	,
cord-326027-58whwspe	39	9	adenovirus	adenovirus	NN
cord-326027-58whwspe	39	10	,	,	,
cord-326027-58whwspe	39	11	ASFV	ASFV	NNP
cord-326027-58whwspe	39	12	and	and	CC
cord-326027-58whwspe	39	13	rabies	rabies	NN
cord-326027-58whwspe	39	14	virus	virus	NN
cord-326027-58whwspe	39	15	are	be	VBP
cord-326027-58whwspe	39	16	thought	think	VBN
cord-326027-58whwspe	39	17	to	to	TO
cord-326027-58whwspe	39	18	use	use	VB
cord-326027-58whwspe	39	19	dynein	dynein	NNP
cord-326027-58whwspe	39	20	motors	motor	NNS
cord-326027-58whwspe	39	21	to	to	TO
cord-326027-58whwspe	39	22	travel	travel	VB
cord-326027-58whwspe	39	23	along	along	IN
cord-326027-58whwspe	39	24	microtubule	microtubule	JJ
cord-326027-58whwspe	39	25	network	network	NN
cord-326027-58whwspe	39	26	for	for	IN
cord-326027-58whwspe	39	27	intracellular	intracellular	JJ
cord-326027-58whwspe	39	28	transport	transport	NN
cord-326027-58whwspe	40	1	(	(	-LRB-
cord-326027-58whwspe	40	2	Alonso	Alonso	NNP
cord-326027-58whwspe	40	3	et	et	NNP
cord-326027-58whwspe	40	4	al	al	NNP
cord-326027-58whwspe	40	5	.	.	.
cord-326027-58whwspe	41	1	,	,	,
cord-326027-58whwspe	41	2	2001	2001	CD
cord-326027-58whwspe	41	3	;	;	:
cord-326027-58whwspe	41	4	Raux	Raux	NNP
cord-326027-58whwspe	41	5	et	et	FW
cord-326027-58whwspe	41	6	al	al	NNP
cord-326027-58whwspe	41	7	.	.	NNP
cord-326027-58whwspe	41	8	,	,	,
cord-326027-58whwspe	41	9	2000	2000	CD
cord-326027-58whwspe	41	10	;	;	:
cord-326027-58whwspe	41	11	Sodeik	Sodeik	NNP
cord-326027-58whwspe	41	12	et	et	NNP
cord-326027-58whwspe	41	13	al	al	NNP
cord-326027-58whwspe	41	14	.	.	NNP
cord-326027-58whwspe	41	15	,	,	,
cord-326027-58whwspe	41	16	1997	1997	CD
cord-326027-58whwspe	42	1	;	;	:
cord-326027-58whwspe	42	2	Suomalainen	Suomalainen	NNP
cord-326027-58whwspe	42	3	et	et	NNP
cord-326027-58whwspe	42	4	al	al	NNP
cord-326027-58whwspe	42	5	.	.	NNP
cord-326027-58whwspe	42	6	,	,	,
cord-326027-58whwspe	42	7	1999	1999	CD
cord-326027-58whwspe	42	8	)	)	-RRB-
cord-326027-58whwspe	42	9	.	.	.
cord-326027-58whwspe	43	1	ASFV	ASFV	NNP
cord-326027-58whwspe	43	2	interacts	interact	VBZ
cord-326027-58whwspe	43	3	with	with	IN
cord-326027-58whwspe	43	4	the	the	DT
cord-326027-58whwspe	43	5	dynein	dynein	NNP
cord-326027-58whwspe	43	6	motor	motor	NN
cord-326027-58whwspe	43	7	complex	complex	NN
cord-326027-58whwspe	43	8	through	through	IN
cord-326027-58whwspe	43	9	the	the	DT
cord-326027-58whwspe	43	10	structural	structural	JJ
cord-326027-58whwspe	43	11	virus	virus	NN
cord-326027-58whwspe	43	12	protein	protein	NN
cord-326027-58whwspe	43	13	p54	p54	CD
cord-326027-58whwspe	43	14	(	(	-LRB-
cord-326027-58whwspe	44	1	Alonso	Alonso	NNP
cord-326027-58whwspe	44	2	et	et	NNP
cord-326027-58whwspe	44	3	al	al	NNP
cord-326027-58whwspe	44	4	.	.	NNP
cord-326027-58whwspe	44	5	,	,	,
cord-326027-58whwspe	44	6	2001	2001	CD
cord-326027-58whwspe	44	7	)	)	-RRB-
cord-326027-58whwspe	44	8	.	.	.
cord-326027-58whwspe	45	1	To	to	TO
cord-326027-58whwspe	45	2	further	further	RB
cord-326027-58whwspe	45	3	characterize	characterize	VB
cord-326027-58whwspe	45	4	the	the	DT
cord-326027-58whwspe	45	5	intracellular	intracellular	JJ
cord-326027-58whwspe	45	6	trafficking	trafficking	NN
cord-326027-58whwspe	45	7	of	of	IN
cord-326027-58whwspe	45	8	some	some	DT
cord-326027-58whwspe	45	9	of	of	IN
cord-326027-58whwspe	45	10	these	these	DT
cord-326027-58whwspe	45	11	viruses	virus	NNS
cord-326027-58whwspe	45	12	,	,	,
cord-326027-58whwspe	45	13	several	several	JJ
cord-326027-58whwspe	45	14	fluorescence	fluorescence	NN
cord-326027-58whwspe	45	15	-	-	HYPH
cord-326027-58whwspe	45	16	based	base	VBN
cord-326027-58whwspe	45	17	methods	method	NNS
cord-326027-58whwspe	45	18	have	have	VBP
cord-326027-58whwspe	45	19	been	be	VBN
cord-326027-58whwspe	45	20	developed	develop	VBN
cord-326027-58whwspe	45	21	allowing	allow	VBG
cord-326027-58whwspe	45	22	the	the	DT
cord-326027-58whwspe	45	23	detection	detection	NN
cord-326027-58whwspe	45	24	and	and	CC
cord-326027-58whwspe	45	25	characterization	characterization	NN
cord-326027-58whwspe	45	26	of	of	IN
cord-326027-58whwspe	45	27	intracellular	intracellular	JJ
cord-326027-58whwspe	45	28	complexes	complex	NNS
cord-326027-58whwspe	45	29	of	of	IN
cord-326027-58whwspe	45	30	viral	viral	JJ
cord-326027-58whwspe	45	31	origin	origin	NN
cord-326027-58whwspe	45	32	.	.	.
cord-326027-58whwspe	46	1	Importantly	importantly	RB
cord-326027-58whwspe	46	2	,	,	,
cord-326027-58whwspe	46	3	several	several	JJ
cord-326027-58whwspe	46	4	of	of	IN
cord-326027-58whwspe	46	5	these	these	DT
cord-326027-58whwspe	46	6	labeling	labeling	NN
cord-326027-58whwspe	46	7	methods	method	NNS
cord-326027-58whwspe	46	8	allow	allow	VBP
cord-326027-58whwspe	46	9	the	the	DT
cord-326027-58whwspe	46	10	visualization	visualization	NN
cord-326027-58whwspe	46	11	of	of	IN
cord-326027-58whwspe	46	12	individual	individual	JJ
cord-326027-58whwspe	46	13	virions	virion	NNS
cord-326027-58whwspe	46	14	in	in	IN
cord-326027-58whwspe	46	15	living	living	NN
cord-326027-58whwspe	46	16	cells	cell	NNS
cord-326027-58whwspe	46	17	(	(	-LRB-
cord-326027-58whwspe	46	18	Smith	Smith	NNP
cord-326027-58whwspe	46	19	and	and	CC
cord-326027-58whwspe	46	20	Enquist	Enquist	NNP
cord-326027-58whwspe	46	21	,	,	,
cord-326027-58whwspe	46	22	2002	2002	CD
cord-326027-58whwspe	46	23	)	)	-RRB-
cord-326027-58whwspe	46	24	.	.	.
cord-326027-58whwspe	47	1	These	these	DT
cord-326027-58whwspe	47	2	studies	study	NNS
cord-326027-58whwspe	47	3	suggest	suggest	VBP
cord-326027-58whwspe	47	4	that	that	IN
cord-326027-58whwspe	47	5	the	the	DT
cord-326027-58whwspe	47	6	intracellular	intracellular	JJ
cord-326027-58whwspe	47	7	trafficking	trafficking	NN
cord-326027-58whwspe	47	8	of	of	IN
cord-326027-58whwspe	47	9	these	these	DT
cord-326027-58whwspe	47	10	viruses	virus	NNS
cord-326027-58whwspe	47	11	is	be	VBZ
cord-326027-58whwspe	47	12	a	a	DT
cord-326027-58whwspe	47	13	highly	highly	RB
cord-326027-58whwspe	47	14	ordered	order	VBN
cord-326027-58whwspe	47	15	process	process	NN
cord-326027-58whwspe	47	16	using	use	VBG
cord-326027-58whwspe	47	17	cellular	cellular	JJ
cord-326027-58whwspe	47	18	motor	motor	NN
cord-326027-58whwspe	47	19	pathways	pathway	NNS
cord-326027-58whwspe	47	20	.	.	.
cord-326027-58whwspe	48	1	Similarly	similarly	RB
cord-326027-58whwspe	48	2	,	,	,
cord-326027-58whwspe	48	3	with	with	IN
cord-326027-58whwspe	48	4	tagged	tag	VBN
cord-326027-58whwspe	48	5	viruses	virus	NNS
cord-326027-58whwspe	48	6	it	-PRON-	PRP
cord-326027-58whwspe	48	7	would	would	MD
cord-326027-58whwspe	48	8	be	be	VB
cord-326027-58whwspe	48	9	possible	possible	JJ
cord-326027-58whwspe	48	10	to	to	TO
cord-326027-58whwspe	48	11	visualize	visualize	VB
cord-326027-58whwspe	48	12	virus	virus	NN
cord-326027-58whwspe	48	13	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	48	14	and	and	CC
cord-326027-58whwspe	48	15	cytopathic	cytopathic	JJ
cord-326027-58whwspe	48	16	effects	effect	NNS
cord-326027-58whwspe	48	17	produced	produce	VBN
cord-326027-58whwspe	48	18	in	in	IN
cord-326027-58whwspe	48	19	the	the	DT
cord-326027-58whwspe	48	20	cells	cell	NNS
cord-326027-58whwspe	48	21	as	as	IN
cord-326027-58whwspe	48	22	infection	infection	NN
cord-326027-58whwspe	48	23	progresses	progress	VBZ
cord-326027-58whwspe	48	24	.	.	.
cord-326027-58whwspe	49	1	In	in	IN
cord-326027-58whwspe	49	2	this	this	DT
cord-326027-58whwspe	49	3	report	report	NN
cord-326027-58whwspe	49	4	,	,	,
cord-326027-58whwspe	49	5	we	-PRON-	PRP
cord-326027-58whwspe	49	6	describe	describe	VBP
cord-326027-58whwspe	49	7	the	the	DT
cord-326027-58whwspe	49	8	construction	construction	NN
cord-326027-58whwspe	49	9	of	of	IN
cord-326027-58whwspe	49	10	an	an	DT
cord-326027-58whwspe	49	11	ASF	ASF	NNP
cord-326027-58whwspe	49	12	recombinant	recombinant	JJ
cord-326027-58whwspe	49	13	virus	virus	NN
cord-326027-58whwspe	49	14	,	,	,
cord-326027-58whwspe	49	15	in	in	IN
cord-326027-58whwspe	49	16	which	which	WDT
cord-326027-58whwspe	49	17	we	-PRON-	PRP
cord-326027-58whwspe	49	18	have	have	VBP
cord-326027-58whwspe	49	19	exchanged	exchange	VBN
cord-326027-58whwspe	49	20	the	the	DT
cord-326027-58whwspe	49	21	single	single	JJ
cord-326027-58whwspe	49	22	copy	copy	NN
cord-326027-58whwspe	49	23	of	of	IN
cord-326027-58whwspe	49	24	the	the	DT
cord-326027-58whwspe	49	25	p54	p54	NN
cord-326027-58whwspe	49	26	open	open	JJ
cord-326027-58whwspe	49	27	reading	reading	NN
cord-326027-58whwspe	49	28	frame	frame	NN
cord-326027-58whwspe	49	29	in	in	IN
cord-326027-58whwspe	49	30	the	the	DT
cord-326027-58whwspe	49	31	ASFV	ASFV	NNP
cord-326027-58whwspe	49	32	genome	genome	NN
cord-326027-58whwspe	49	33	,	,	,
cord-326027-58whwspe	49	34	gene	gene	NN
cord-326027-58whwspe	49	35	E183L	E183L	NNP
cord-326027-58whwspe	49	36	(	(	-LRB-
cord-326027-58whwspe	49	37	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	49	38	et	et	NNP
cord-326027-58whwspe	49	39	al	al	NNP
cord-326027-58whwspe	49	40	.	.	NNP
cord-326027-58whwspe	49	41	,	,	,
cord-326027-58whwspe	49	42	1994	1994	CD
cord-326027-58whwspe	49	43	)	)	-RRB-
cord-326027-58whwspe	49	44	with	with	IN
cord-326027-58whwspe	49	45	a	a	DT
cord-326027-58whwspe	49	46	chimeric	chimeric	JJ
cord-326027-58whwspe	49	47	gene	gene	NN
cord-326027-58whwspe	49	48	encoding	encode	VBG
cord-326027-58whwspe	49	49	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	49	50	.	.	.
cord-326027-58whwspe	50	1	Interestingly	interestingly	RB
cord-326027-58whwspe	50	2	,	,	,
cord-326027-58whwspe	50	3	this	this	DT
cord-326027-58whwspe	50	4	virus	virus	NN
cord-326027-58whwspe	50	5	is	be	VBZ
cord-326027-58whwspe	50	6	fully	fully	RB
cord-326027-58whwspe	50	7	viable	viable	JJ
cord-326027-58whwspe	50	8	and	and	CC
cord-326027-58whwspe	50	9	exhibits	exhibit	VBZ
cord-326027-58whwspe	50	10	growth	growth	NN
cord-326027-58whwspe	50	11	kinetics	kinetic	NNS
cord-326027-58whwspe	50	12	similar	similar	JJ
cord-326027-58whwspe	50	13	to	to	IN
cord-326027-58whwspe	50	14	those	those	DT
cord-326027-58whwspe	50	15	of	of	IN
cord-326027-58whwspe	50	16	its	-PRON-	PRP$
cord-326027-58whwspe	50	17	parental	parental	JJ
cord-326027-58whwspe	50	18	virus	virus	NN
cord-326027-58whwspe	50	19	.	.	.
cord-326027-58whwspe	51	1	Moreover	moreover	RB
cord-326027-58whwspe	51	2	,	,	,
cord-326027-58whwspe	51	3	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	51	4	is	be	VBZ
cord-326027-58whwspe	51	5	incorporated	incorporate	VBN
cord-326027-58whwspe	51	6	into	into	IN
cord-326027-58whwspe	51	7	the	the	DT
cord-326027-58whwspe	51	8	virus	virus	NN
cord-326027-58whwspe	51	9	particle	particle	NN
cord-326027-58whwspe	51	10	with	with	IN
cord-326027-58whwspe	51	11	the	the	DT
cord-326027-58whwspe	51	12	same	same	JJ
cord-326027-58whwspe	51	13	efficiency	efficiency	NN
cord-326027-58whwspe	51	14	as	as	IN
cord-326027-58whwspe	51	15	p54	p54	CD
cord-326027-58whwspe	51	16	.	.	.
cord-326027-58whwspe	52	1	The	the	DT
cord-326027-58whwspe	52	2	presence	presence	NN
cord-326027-58whwspe	52	3	of	of	IN
cord-326027-58whwspe	52	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	52	5	in	in	IN
cord-326027-58whwspe	52	6	the	the	DT
cord-326027-58whwspe	52	7	virion	virion	NN
cord-326027-58whwspe	52	8	results	result	NNS
cord-326027-58whwspe	52	9	in	in	IN
cord-326027-58whwspe	52	10	fluorescent	fluorescent	NN
cord-326027-58whwspe	52	11	particles	particle	NNS
cord-326027-58whwspe	52	12	which	which	WDT
cord-326027-58whwspe	52	13	are	be	VBP
cord-326027-58whwspe	52	14	readily	readily	RB
cord-326027-58whwspe	52	15	visualized	visualize	VBN
cord-326027-58whwspe	52	16	with	with	IN
cord-326027-58whwspe	52	17	a	a	DT
cord-326027-58whwspe	52	18	fluorescence	fluorescence	NN
cord-326027-58whwspe	52	19	microscope	microscope	NN
cord-326027-58whwspe	52	20	,	,	,
cord-326027-58whwspe	52	21	allowing	allow	VBG
cord-326027-58whwspe	52	22	visualization	visualization	NN
cord-326027-58whwspe	52	23	of	of	IN
cord-326027-58whwspe	52	24	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	52	25	within	within	IN
cord-326027-58whwspe	52	26	live	live	JJ
cord-326027-58whwspe	52	27	cells	cell	NNS
cord-326027-58whwspe	52	28	.	.	.
cord-326027-58whwspe	53	1	As	as	IN
cord-326027-58whwspe	53	2	a	a	DT
cord-326027-58whwspe	53	3	consequence	consequence	NN
cord-326027-58whwspe	53	4	of	of	IN
cord-326027-58whwspe	53	5	such	such	JJ
cord-326027-58whwspe	53	6	sensitive	sensitive	JJ
cord-326027-58whwspe	53	7	detection	detection	NN
cord-326027-58whwspe	53	8	of	of	IN
cord-326027-58whwspe	53	9	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	53	10	throughout	throughout	IN
cord-326027-58whwspe	53	11	infection	infection	NN
cord-326027-58whwspe	53	12	,	,	,
cord-326027-58whwspe	53	13	we	-PRON-	PRP
cord-326027-58whwspe	53	14	have	have	VBP
cord-326027-58whwspe	53	15	been	be	VBN
cord-326027-58whwspe	53	16	able	able	JJ
cord-326027-58whwspe	53	17	to	to	TO
cord-326027-58whwspe	53	18	use	use	VB
cord-326027-58whwspe	53	19	time	time	NN
cord-326027-58whwspe	53	20	lapse	lapse	NN
cord-326027-58whwspe	53	21	confocal	confocal	JJ
cord-326027-58whwspe	53	22	microscopy	microscopy	NNS
cord-326027-58whwspe	53	23	to	to	TO
cord-326027-58whwspe	53	24	monitor	monitor	VB
cord-326027-58whwspe	53	25	the	the	DT
cord-326027-58whwspe	53	26	trafficking	trafficking	NN
cord-326027-58whwspe	53	27	of	of	IN
cord-326027-58whwspe	53	28	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	53	29	within	within	IN
cord-326027-58whwspe	53	30	individual	individual	JJ
cord-326027-58whwspe	53	31	cells	cell	NNS
cord-326027-58whwspe	53	32	.	.	.
cord-326027-58whwspe	54	1	We	-PRON-	PRP
cord-326027-58whwspe	54	2	present	present	VBP
cord-326027-58whwspe	54	3	those	those	DT
cord-326027-58whwspe	54	4	results	result	NNS
cord-326027-58whwspe	54	5	as	as	IN
cord-326027-58whwspe	54	6	subsequent	subsequent	JJ
cord-326027-58whwspe	54	7	images	image	NNS
cord-326027-58whwspe	54	8	and	and	CC
cord-326027-58whwspe	54	9	time	time	NN
cord-326027-58whwspe	54	10	lapse	lapse	NN
cord-326027-58whwspe	54	11	animations	animation	NNS
cord-326027-58whwspe	54	12	.	.	.
cord-326027-58whwspe	55	1	Thus	thus	RB
cord-326027-58whwspe	55	2	,	,	,
cord-326027-58whwspe	55	3	we	-PRON-	PRP
cord-326027-58whwspe	55	4	have	have	VBP
cord-326027-58whwspe	55	5	generated	generate	VBN
cord-326027-58whwspe	55	6	a	a	DT
cord-326027-58whwspe	55	7	reagent	reagent	NN
cord-326027-58whwspe	55	8	which	which	WDT
cord-326027-58whwspe	55	9	will	will	MD
cord-326027-58whwspe	55	10	enable	enable	VB
cord-326027-58whwspe	55	11	the	the	DT
cord-326027-58whwspe	55	12	visualization	visualization	NN
cord-326027-58whwspe	55	13	of	of	IN
cord-326027-58whwspe	55	14	several	several	JJ
cord-326027-58whwspe	55	15	aspects	aspect	NNS
cord-326027-58whwspe	55	16	of	of	IN
cord-326027-58whwspe	55	17	ASFV	ASFV	NNP
cord-326027-58whwspe	55	18	infection	infection	NN
cord-326027-58whwspe	55	19	in	in	IN
cord-326027-58whwspe	55	20	live	live	JJ
cord-326027-58whwspe	55	21	cells	cell	NNS
cord-326027-58whwspe	55	22	,	,	,
cord-326027-58whwspe	55	23	including	include	VBG
cord-326027-58whwspe	55	24	virus	virus	NN
cord-326027-58whwspe	55	25	entry	entry	NN
cord-326027-58whwspe	55	26	,	,	,
cord-326027-58whwspe	55	27	assembly	assembly	NN
cord-326027-58whwspe	55	28	,	,	,
cord-326027-58whwspe	55	29	trafficking	trafficking	NN
cord-326027-58whwspe	55	30	and	and	CC
cord-326027-58whwspe	55	31	apoptotic	apoptotic	JJ
cord-326027-58whwspe	55	32	cell	cell	NN
cord-326027-58whwspe	55	33	death	death	NN
cord-326027-58whwspe	55	34	.	.	.
cord-326027-58whwspe	56	1	The	the	DT
cord-326027-58whwspe	56	2	main	main	JJ
cord-326027-58whwspe	56	3	goal	goal	NN
cord-326027-58whwspe	56	4	of	of	IN
cord-326027-58whwspe	56	5	the	the	DT
cord-326027-58whwspe	56	6	molecular	molecular	JJ
cord-326027-58whwspe	56	7	manipulations	manipulation	NNS
cord-326027-58whwspe	56	8	described	describe	VBN
cord-326027-58whwspe	56	9	below	below	RB
cord-326027-58whwspe	56	10	was	be	VBD
cord-326027-58whwspe	56	11	to	to	TO
cord-326027-58whwspe	56	12	generate	generate	VB
cord-326027-58whwspe	56	13	a	a	DT
cord-326027-58whwspe	56	14	tagged	tagged	NNP
cord-326027-58whwspe	56	15	ASF	ASF	NNP
cord-326027-58whwspe	56	16	virion	virion	NN
cord-326027-58whwspe	56	17	that	that	WDT
cord-326027-58whwspe	56	18	allowed	allow	VBD
cord-326027-58whwspe	56	19	us	-PRON-	PRP
cord-326027-58whwspe	56	20	monitoring	monitor	VBG
cord-326027-58whwspe	56	21	the	the	DT
cord-326027-58whwspe	56	22	infection	infection	NN
cord-326027-58whwspe	56	23	in	in	IN
cord-326027-58whwspe	56	24	living	living	NN
cord-326027-58whwspe	56	25	cells	cell	NNS
cord-326027-58whwspe	56	26	.	.	.
cord-326027-58whwspe	57	1	We	-PRON-	PRP
cord-326027-58whwspe	57	2	selected	select	VBD
cord-326027-58whwspe	57	3	the	the	DT
cord-326027-58whwspe	57	4	ASFV	ASFV	NNP
cord-326027-58whwspe	57	5	envelope	envelope	NN
cord-326027-58whwspe	57	6	protein	protein	NN
cord-326027-58whwspe	57	7	p54	p54	NN
cord-326027-58whwspe	57	8	,	,	,
cord-326027-58whwspe	57	9	encoded	encode	VBN
cord-326027-58whwspe	57	10	by	by	IN
cord-326027-58whwspe	57	11	the	the	DT
cord-326027-58whwspe	57	12	essential	essential	JJ
cord-326027-58whwspe	57	13	E183L	E183L	NNP
cord-326027-58whwspe	57	14	gene	gene	NN
cord-326027-58whwspe	57	15	(	(	-LRB-
cord-326027-58whwspe	57	16	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	57	17	et	et	NNP
cord-326027-58whwspe	57	18	al	al	NNP
cord-326027-58whwspe	57	19	.	.	NNP
cord-326027-58whwspe	57	20	,	,	,
cord-326027-58whwspe	57	21	1994	1994	CD
cord-326027-58whwspe	57	22	)	)	-RRB-
cord-326027-58whwspe	57	23	,	,	,
cord-326027-58whwspe	57	24	to	to	TO
cord-326027-58whwspe	57	25	drive	drive	VB
cord-326027-58whwspe	57	26	the	the	DT
cord-326027-58whwspe	57	27	EGFP	EGFP	NNP
cord-326027-58whwspe	57	28	incorporation	incorporation	NN
cord-326027-58whwspe	57	29	into	into	IN
cord-326027-58whwspe	57	30	the	the	DT
cord-326027-58whwspe	57	31	virus	virus	NN
cord-326027-58whwspe	57	32	envelope	envelope	NN
cord-326027-58whwspe	57	33	.	.	.
cord-326027-58whwspe	58	1	Then	then	RB
cord-326027-58whwspe	58	2	,	,	,
cord-326027-58whwspe	58	3	we	-PRON-	PRP
cord-326027-58whwspe	58	4	replaced	replace	VBD
cord-326027-58whwspe	58	5	the	the	DT
cord-326027-58whwspe	58	6	protein	protein	NN
cord-326027-58whwspe	58	7	p54	p54	NN
cord-326027-58whwspe	58	8	in	in	IN
cord-326027-58whwspe	58	9	the	the	DT
cord-326027-58whwspe	58	10	virus	virus	NN
cord-326027-58whwspe	58	11	particle	particle	NN
cord-326027-58whwspe	58	12	by	by	IN
cord-326027-58whwspe	58	13	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	58	14	fusion	fusion	NN
cord-326027-58whwspe	58	15	chimera	chimera	NN
cord-326027-58whwspe	58	16	in	in	IN
cord-326027-58whwspe	58	17	two	two	CD
cord-326027-58whwspe	58	18	steps	step	NNS
cord-326027-58whwspe	58	19	.	.	.
cord-326027-58whwspe	59	1	First	first	RB
cord-326027-58whwspe	59	2	,	,	,
cord-326027-58whwspe	59	3	we	-PRON-	PRP
cord-326027-58whwspe	59	4	introduced	introduce	VBD
cord-326027-58whwspe	59	5	in	in	IN
cord-326027-58whwspe	59	6	the	the	DT
cord-326027-58whwspe	59	7	TK	TK	NNP
cord-326027-58whwspe	59	8	gene	gene	NN
cord-326027-58whwspe	59	9	of	of	IN
cord-326027-58whwspe	59	10	the	the	DT
cord-326027-58whwspe	59	11	virus	virus	NN
cord-326027-58whwspe	59	12	the	the	DT
cord-326027-58whwspe	59	13	sequence	sequence	NN
cord-326027-58whwspe	59	14	encoding	encode	VBG
cord-326027-58whwspe	59	15	the	the	DT
cord-326027-58whwspe	59	16	fusion	fusion	NN
cord-326027-58whwspe	59	17	protein	protein	NN
cord-326027-58whwspe	59	18	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	59	19	under	under	IN
cord-326027-58whwspe	59	20	the	the	DT
cord-326027-58whwspe	59	21	control	control	NN
cord-326027-58whwspe	59	22	of	of	IN
cord-326027-58whwspe	59	23	p54	p54	NN
cord-326027-58whwspe	59	24	promoter	promoter	NN
cord-326027-58whwspe	59	25	(	(	-LRB-
cord-326027-58whwspe	59	26	Fig	Fig	NNP
cord-326027-58whwspe	59	27	.	.	.
cord-326027-58whwspe	59	28	1a	1a	CD
cord-326027-58whwspe	59	29	)	)	-RRB-
cord-326027-58whwspe	59	30	.	.	.
cord-326027-58whwspe	60	1	Recombinant	recombinant	JJ
cord-326027-58whwspe	60	2	virus	virus	NN
cord-326027-58whwspe	60	3	plaques	plaque	NNS
cord-326027-58whwspe	60	4	exhibiting	exhibit	VBG
cord-326027-58whwspe	60	5	green	green	JJ
cord-326027-58whwspe	60	6	fluorescence	fluorescence	NN
cord-326027-58whwspe	60	7	were	be	VBD
cord-326027-58whwspe	60	8	picked	pick	VBN
cord-326027-58whwspe	60	9	and	and	CC
cord-326027-58whwspe	60	10	individual	individual	JJ
cord-326027-58whwspe	60	11	recombinant	recombinant	JJ
cord-326027-58whwspe	60	12	viruses	virus	NNS
cord-326027-58whwspe	60	13	were	be	VBD
cord-326027-58whwspe	60	14	further	further	JJ
cord-326027-58whwspe	60	15	plaque	plaque	NN
cord-326027-58whwspe	60	16	purified	purify	VBN
cord-326027-58whwspe	60	17	by	by	IN
cord-326027-58whwspe	60	18	limiting	limit	VBG
cord-326027-58whwspe	60	19	dilution	dilution	NN
cord-326027-58whwspe	60	20	and	and	CC
cord-326027-58whwspe	60	21	screened	screen	VBN
cord-326027-58whwspe	60	22	by	by	IN
cord-326027-58whwspe	60	23	PCR	PCR	NNP
cord-326027-58whwspe	60	24	and	and	CC
cord-326027-58whwspe	60	25	Southern	southern	JJ
cord-326027-58whwspe	60	26	blot	blot	NN
cord-326027-58whwspe	60	27	to	to	TO
cord-326027-58whwspe	60	28	confirm	confirm	VB
cord-326027-58whwspe	60	29	the	the	DT
cord-326027-58whwspe	60	30	presence	presence	NN
cord-326027-58whwspe	60	31	of	of	IN
cord-326027-58whwspe	60	32	the	the	DT
cord-326027-58whwspe	60	33	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	60	34	coding	code	VBG
cord-326027-58whwspe	60	35	sequence	sequence	NN
cord-326027-58whwspe	60	36	in	in	IN
cord-326027-58whwspe	60	37	the	the	DT
cord-326027-58whwspe	60	38	TK	TK	NNP
cord-326027-58whwspe	60	39	locus	locus	NN
cord-326027-58whwspe	60	40	,	,	,
cord-326027-58whwspe	60	41	as	as	IN
cord-326027-58whwspe	60	42	expected	expect	VBN
cord-326027-58whwspe	60	43	(	(	-LRB-
cord-326027-58whwspe	60	44	Fig	Fig	NNP
cord-326027-58whwspe	60	45	.	.	.
cord-326027-58whwspe	60	46	1b	1b	CD
cord-326027-58whwspe	60	47	)	)	-RRB-
cord-326027-58whwspe	60	48	.	.	.
cord-326027-58whwspe	61	1	The	the	DT
cord-326027-58whwspe	61	2	recombinant	recombinant	JJ
cord-326027-58whwspe	61	3	virus	virus	NN
cord-326027-58whwspe	61	4	obtained	obtain	VBN
cord-326027-58whwspe	61	5	was	be	VBD
cord-326027-58whwspe	61	6	designated	designate	VBN
cord-326027-58whwspe	61	7	as	as	IN
cord-326027-58whwspe	61	8	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	61	9	and	and	CC
cord-326027-58whwspe	61	10	used	use	VBD
cord-326027-58whwspe	61	11	to	to	TO
cord-326027-58whwspe	61	12	infect	infect	VB
cord-326027-58whwspe	61	13	cells	cell	NNS
cord-326027-58whwspe	61	14	to	to	TO
cord-326027-58whwspe	61	15	visualize	visualize	VB
cord-326027-58whwspe	61	16	the	the	DT
cord-326027-58whwspe	61	17	fluorescence	fluorescence	NN
cord-326027-58whwspe	61	18	properties	property	NNS
cord-326027-58whwspe	61	19	of	of	IN
cord-326027-58whwspe	61	20	individual	individual	JJ
cord-326027-58whwspe	61	21	virus	virus	NN
cord-326027-58whwspe	61	22	clones	clone	NNS
cord-326027-58whwspe	61	23	(	(	-LRB-
cord-326027-58whwspe	61	24	Figs	fig	NNS
cord-326027-58whwspe	61	25	.	.	.
cord-326027-58whwspe	62	1	1c	1c	NNP
cord-326027-58whwspe	62	2	and	and	CC
cord-326027-58whwspe	62	3	d	d	NNP
cord-326027-58whwspe	62	4	)	)	-RRB-
cord-326027-58whwspe	62	5	.	.	.
cord-326027-58whwspe	63	1	Moreover	moreover	RB
cord-326027-58whwspe	63	2	,	,	,
cord-326027-58whwspe	63	3	the	the	DT
cord-326027-58whwspe	63	4	use	use	NN
cord-326027-58whwspe	63	5	of	of	IN
cord-326027-58whwspe	63	6	EGFP	EGFP	NNP
cord-326027-58whwspe	63	7	as	as	IN
cord-326027-58whwspe	63	8	marker	marker	NN
cord-326027-58whwspe	63	9	gene	gene	NN
cord-326027-58whwspe	63	10	allowed	allow	VBD
cord-326027-58whwspe	63	11	a	a	DT
cord-326027-58whwspe	63	12	faster	fast	JJR
cord-326027-58whwspe	63	13	detection	detection	NN
cord-326027-58whwspe	63	14	of	of	IN
cord-326027-58whwspe	63	15	recombinant	recombinant	JJ
cord-326027-58whwspe	63	16	viral	viral	JJ
cord-326027-58whwspe	63	17	plaques	plaque	NNS
cord-326027-58whwspe	63	18	when	when	WRB
cord-326027-58whwspe	63	19	compared	compare	VBN
cord-326027-58whwspe	63	20	to	to	IN
cord-326027-58whwspe	63	21	wild	wild	JJ
cord-326027-58whwspe	63	22	-	-	HYPH
cord-326027-58whwspe	63	23	type	type	NN
cord-326027-58whwspe	63	24	virus	virus	NN
cord-326027-58whwspe	63	25	(	(	-LRB-
cord-326027-58whwspe	63	26	data	datum	NNS
cord-326027-58whwspe	63	27	not	not	RB
cord-326027-58whwspe	63	28	shown	show	VBN
cord-326027-58whwspe	63	29	)	)	-RRB-
cord-326027-58whwspe	63	30	.	.	.
cord-326027-58whwspe	64	1	EGFP	EGFP	NNP
cord-326027-58whwspe	64	2	fusion	fusion	NN
cord-326027-58whwspe	64	3	to	to	IN
cord-326027-58whwspe	64	4	a	a	DT
cord-326027-58whwspe	64	5	specific	specific	JJ
cord-326027-58whwspe	64	6	protein	protein	NN
cord-326027-58whwspe	64	7	often	often	RB
cord-326027-58whwspe	64	8	modifies	modify	VBZ
cord-326027-58whwspe	64	9	its	-PRON-	PRP$
cord-326027-58whwspe	64	10	inherent	inherent	JJ
cord-326027-58whwspe	64	11	properties	property	NNS
cord-326027-58whwspe	64	12	,	,	,
cord-326027-58whwspe	64	13	so	so	RB
cord-326027-58whwspe	64	14	we	-PRON-	PRP
cord-326027-58whwspe	64	15	first	first	RB
cord-326027-58whwspe	64	16	determined	determine	VBD
cord-326027-58whwspe	64	17	whether	whether	IN
cord-326027-58whwspe	64	18	the	the	DT
cord-326027-58whwspe	64	19	addition	addition	NN
cord-326027-58whwspe	64	20	of	of	IN
cord-326027-58whwspe	64	21	EGFP	EGFP	NNP
cord-326027-58whwspe	64	22	to	to	IN
cord-326027-58whwspe	64	23	the	the	DT
cord-326027-58whwspe	64	24	C	c	NN
cord-326027-58whwspe	64	25	-	-	HYPH
cord-326027-58whwspe	64	26	terminus	terminus	NN
cord-326027-58whwspe	64	27	of	of	IN
cord-326027-58whwspe	64	28	p54	p54	NN
cord-326027-58whwspe	64	29	could	could	MD
cord-326027-58whwspe	64	30	affect	affect	VB
cord-326027-58whwspe	64	31	the	the	DT
cord-326027-58whwspe	64	32	usual	usual	JJ
cord-326027-58whwspe	64	33	subcellular	subcellular	JJ
cord-326027-58whwspe	64	34	distribution	distribution	NN
cord-326027-58whwspe	64	35	of	of	IN
cord-326027-58whwspe	64	36	this	this	DT
cord-326027-58whwspe	64	37	protein	protein	NN
cord-326027-58whwspe	64	38	.	.	.
cord-326027-58whwspe	65	1	Then	then	RB
cord-326027-58whwspe	65	2	,	,	,
cord-326027-58whwspe	65	3	Vero	Vero	NNP
cord-326027-58whwspe	65	4	cells	cell	NNS
cord-326027-58whwspe	65	5	were	be	VBD
cord-326027-58whwspe	65	6	infected	infect	VBN
cord-326027-58whwspe	65	7	with	with	IN
cord-326027-58whwspe	65	8	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	65	9	or	or	CC
cord-326027-58whwspe	65	10	wild	wild	JJ
cord-326027-58whwspe	65	11	-	-	HYPH
cord-326027-58whwspe	65	12	type	type	NN
cord-326027-58whwspe	65	13	virus	virus	NN
cord-326027-58whwspe	65	14	(	(	-LRB-
cord-326027-58whwspe	65	15	BA71V	ba71v	NN
cord-326027-58whwspe	65	16	)	)	-RRB-
cord-326027-58whwspe	65	17	and	and	CC
cord-326027-58whwspe	66	1	subcellular	subcellular	JJ
cord-326027-58whwspe	66	2	distributions	distribution	NNS
cord-326027-58whwspe	66	3	of	of	IN
cord-326027-58whwspe	66	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	66	5	and	and	CC
cord-326027-58whwspe	66	6	p54	p54	CD
cord-326027-58whwspe	66	7	were	be	VBD
cord-326027-58whwspe	66	8	examined	examine	VBN
cord-326027-58whwspe	66	9	by	by	IN
cord-326027-58whwspe	66	10	conventional	conventional	JJ
cord-326027-58whwspe	66	11	immunofluorescence	immunofluorescence	NN
cord-326027-58whwspe	66	12	microscopy	microscopy	NN
cord-326027-58whwspe	66	13	(	(	-LRB-
cord-326027-58whwspe	66	14	Figs	fig	NNS
cord-326027-58whwspe	66	15	.	.	.
cord-326027-58whwspe	67	1	1e	1e	NNP
cord-326027-58whwspe	67	2	-	-	HYPH
cord-326027-58whwspe	67	3	j	j	NNP
cord-326027-58whwspe	67	4	)	)	-RRB-
cord-326027-58whwspe	67	5	.	.	.
cord-326027-58whwspe	68	1	No	no	DT
cord-326027-58whwspe	68	2	differences	difference	NNS
cord-326027-58whwspe	68	3	were	be	VBD
cord-326027-58whwspe	68	4	observed	observe	VBN
cord-326027-58whwspe	68	5	when	when	WRB
cord-326027-58whwspe	68	6	compared	compare	VBN
cord-326027-58whwspe	68	7	,	,	,
cord-326027-58whwspe	68	8	demonstrating	demonstrate	VBG
cord-326027-58whwspe	68	9	that	that	DT
cord-326027-58whwspe	68	10	typical	typical	JJ
cord-326027-58whwspe	68	11	perinuclear	perinuclear	NNP
cord-326027-58whwspe	68	12	accumulation	accumulation	NN
cord-326027-58whwspe	68	13	described	describe	VBN
cord-326027-58whwspe	68	14	for	for	IN
cord-326027-58whwspe	68	15	p54	p54	NN
cord-326027-58whwspe	68	16	in	in	IN
cord-326027-58whwspe	68	17	ASFV	ASFV	NNP
cord-326027-58whwspe	68	18	infection	infection	NN
cord-326027-58whwspe	68	19	was	be	VBD
cord-326027-58whwspe	68	20	not	not	RB
cord-326027-58whwspe	68	21	altered	alter	VBN
cord-326027-58whwspe	68	22	by	by	IN
cord-326027-58whwspe	68	23	the	the	DT
cord-326027-58whwspe	68	24	GFP	GFP	NNP
cord-326027-58whwspe	68	25	fusion	fusion	NN
cord-326027-58whwspe	68	26	.	.	.
cord-326027-58whwspe	69	1	p54	p54	NN
cord-326027-58whwspe	69	2	is	be	VBZ
cord-326027-58whwspe	69	3	an	an	DT
cord-326027-58whwspe	69	4	essential	essential	JJ
cord-326027-58whwspe	69	5	protein	protein	NN
cord-326027-58whwspe	69	6	and	and	CC
cord-326027-58whwspe	69	7	then	then	RB
cord-326027-58whwspe	69	8	to	to	TO
cord-326027-58whwspe	69	9	study	study	VB
cord-326027-58whwspe	69	10	p54-EGFP	p54-egfp	JJ
cord-326027-58whwspe	69	11	functionality	functionality	NN
cord-326027-58whwspe	69	12	in	in	IN
cord-326027-58whwspe	69	13	the	the	DT
cord-326027-58whwspe	69	14	virus	virus	NN
cord-326027-58whwspe	69	15	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	69	16	and	and	CC
cord-326027-58whwspe	69	17	to	to	TO
cord-326027-58whwspe	69	18	increase	increase	VB
cord-326027-58whwspe	69	19	the	the	DT
cord-326027-58whwspe	69	20	number	number	NN
cord-326027-58whwspe	69	21	of	of	IN
cord-326027-58whwspe	69	22	copies	copy	NNS
cord-326027-58whwspe	69	23	of	of	IN
cord-326027-58whwspe	69	24	the	the	DT
cord-326027-58whwspe	69	25	tagged	tagged	NNP
cord-326027-58whwspe	69	26	protein	protein	NN
cord-326027-58whwspe	69	27	in	in	IN
cord-326027-58whwspe	69	28	the	the	DT
cord-326027-58whwspe	69	29	virions	virion	NNS
cord-326027-58whwspe	69	30	,	,	,
cord-326027-58whwspe	69	31	the	the	DT
cord-326027-58whwspe	69	32	wildtype	wildtype	JJ
cord-326027-58whwspe	69	33	E183L	E183L	NNP
cord-326027-58whwspe	69	34	gene	gene	NN
cord-326027-58whwspe	69	35	was	be	VBD
cord-326027-58whwspe	69	36	interrupted	interrupt	VBN
cord-326027-58whwspe	69	37	by	by	IN
cord-326027-58whwspe	69	38	insertion	insertion	NN
cord-326027-58whwspe	69	39	of	of	IN
cord-326027-58whwspe	69	40	the	the	DT
cord-326027-58whwspe	69	41	βgalactosidase	βgalactosidase	NN
cord-326027-58whwspe	69	42	coding	code	VBG
cord-326027-58whwspe	69	43	sequence	sequence	NN
cord-326027-58whwspe	69	44	.	.	.
cord-326027-58whwspe	70	1	This	this	DT
cord-326027-58whwspe	70	2	second	second	JJ
cord-326027-58whwspe	70	3	step	step	NN
cord-326027-58whwspe	70	4	in	in	IN
cord-326027-58whwspe	70	5	recombinant	recombinant	JJ
cord-326027-58whwspe	70	6	virus	virus	NN
cord-326027-58whwspe	70	7	generation	generation	NN
cord-326027-58whwspe	70	8	was	be	VBD
cord-326027-58whwspe	70	9	performed	perform	VBN
cord-326027-58whwspe	70	10	by	by	IN
cord-326027-58whwspe	70	11	homologous	homologous	JJ
cord-326027-58whwspe	70	12	recombination	recombination	NN
cord-326027-58whwspe	70	13	with	with	IN
cord-326027-58whwspe	70	14	vector	vector	NN
cord-326027-58whwspe	70	15	pΔp54	pδp54	XX
cord-326027-58whwspe	70	16	which	which	WDT
cord-326027-58whwspe	70	17	contains	contain	VBZ
cord-326027-58whwspe	70	18	the	the	DT
cord-326027-58whwspe	70	19	β	β	NN
cord-326027-58whwspe	70	20	-	-	HYPH
cord-326027-58whwspe	70	21	galactosidase	galactosidase	NN
cord-326027-58whwspe	70	22	coding	code	VBG
cord-326027-58whwspe	70	23	sequence	sequence	NN
cord-326027-58whwspe	70	24	under	under	IN
cord-326027-58whwspe	70	25	the	the	DT
cord-326027-58whwspe	70	26	control	control	NN
cord-326027-58whwspe	70	27	of	of	IN
cord-326027-58whwspe	70	28	p72	p72	NN
cord-326027-58whwspe	70	29	promoter	promoter	NN
cord-326027-58whwspe	70	30	and	and	CC
cord-326027-58whwspe	70	31	flanqued	flanque	VBN
cord-326027-58whwspe	70	32	by	by	IN
cord-326027-58whwspe	70	33	424	424	CD
cord-326027-58whwspe	70	34	pb	pb	NNP
cord-326027-58whwspe	70	35	and	and	CC
cord-326027-58whwspe	70	36	888	888	CD
cord-326027-58whwspe	70	37	pb	pb	NN
cord-326027-58whwspe	70	38	from	from	IN
cord-326027-58whwspe	70	39	p54	p54	NN
cord-326027-58whwspe	70	40	E183L	E183L	NNP
cord-326027-58whwspe	70	41	right	right	JJ
cord-326027-58whwspe	70	42	and	and	CC
cord-326027-58whwspe	70	43	left	left	JJ
cord-326027-58whwspe	70	44	extremes	extreme	NNS
cord-326027-58whwspe	70	45	,	,	,
cord-326027-58whwspe	70	46	respectively	respectively	RB
cord-326027-58whwspe	70	47	.	.	.
cord-326027-58whwspe	71	1	Resulting	result	VBG
cord-326027-58whwspe	71	2	recombinant	recombinant	JJ
cord-326027-58whwspe	71	3	virus	virus	NN
cord-326027-58whwspe	71	4	plaques	plaque	NNS
cord-326027-58whwspe	71	5	exhibiting	exhibit	VBG
cord-326027-58whwspe	71	6	β	β	NN
cord-326027-58whwspe	71	7	-	-	HYPH
cord-326027-58whwspe	71	8	gal	gal	NN
cord-326027-58whwspe	71	9	activity	activity	NN
cord-326027-58whwspe	71	10	were	be	VBD
cord-326027-58whwspe	71	11	picked	pick	VBN
cord-326027-58whwspe	71	12	and	and	CC
cord-326027-58whwspe	71	13	analyzed	analyze	VBN
cord-326027-58whwspe	71	14	by	by	IN
cord-326027-58whwspe	71	15	PCR	PCR	NNP
cord-326027-58whwspe	71	16	as	as	RB
cord-326027-58whwspe	71	17	well	well	RB
cord-326027-58whwspe	71	18	as	as	IN
cord-326027-58whwspe	71	19	Southern	southern	JJ
cord-326027-58whwspe	71	20	blot	blot	NN
cord-326027-58whwspe	71	21	to	to	TO
cord-326027-58whwspe	71	22	confirm	confirm	VB
cord-326027-58whwspe	71	23	the	the	DT
cord-326027-58whwspe	71	24	interruption	interruption	NN
cord-326027-58whwspe	71	25	of	of	IN
cord-326027-58whwspe	71	26	gene	gene	NN
cord-326027-58whwspe	71	27	E183L	E183L	NNP
cord-326027-58whwspe	71	28	in	in	IN
cord-326027-58whwspe	71	29	the	the	DT
cord-326027-58whwspe	71	30	genome	genome	NN
cord-326027-58whwspe	71	31	of	of	IN
cord-326027-58whwspe	71	32	ASFV	ASFV	NNP
cord-326027-58whwspe	71	33	(	(	-LRB-
cord-326027-58whwspe	71	34	Fig	Fig	NNP
cord-326027-58whwspe	71	35	.	.	.
cord-326027-58whwspe	71	36	1b	1b	CD
cord-326027-58whwspe	71	37	)	)	-RRB-
cord-326027-58whwspe	71	38	.	.	.
cord-326027-58whwspe	72	1	After	after	IN
cord-326027-58whwspe	72	2	isolation	isolation	NN
cord-326027-58whwspe	72	3	by	by	IN
cord-326027-58whwspe	72	4	several	several	JJ
cord-326027-58whwspe	72	5	rounds	round	NNS
cord-326027-58whwspe	72	6	of	of	IN
cord-326027-58whwspe	72	7	plaque	plaque	NN
cord-326027-58whwspe	72	8	purification	purification	NN
cord-326027-58whwspe	72	9	,	,	,
cord-326027-58whwspe	72	10	all	all	DT
cord-326027-58whwspe	72	11	virus	virus	NN
cord-326027-58whwspe	72	12	plaques	plaque	NNS
cord-326027-58whwspe	72	13	exhibited	exhibit	VBD
cord-326027-58whwspe	72	14	both	both	DT
cord-326027-58whwspe	72	15	GFP	GFP	NNP
cord-326027-58whwspe	72	16	and	and	CC
cord-326027-58whwspe	72	17	β	β	NN
cord-326027-58whwspe	72	18	-	-	HYPH
cord-326027-58whwspe	72	19	galactosidase	galactosidase	NN
cord-326027-58whwspe	72	20	activities	activity	NNS
cord-326027-58whwspe	72	21	.	.	.
cord-326027-58whwspe	73	1	The	the	DT
cord-326027-58whwspe	73	2	double	double	JJ
cord-326027-58whwspe	73	3	recombinant	recombinant	JJ
cord-326027-58whwspe	73	4	virus	virus	NN
cord-326027-58whwspe	73	5	finally	finally	RB
cord-326027-58whwspe	73	6	obtained	obtain	VBN
cord-326027-58whwspe	73	7	was	be	VBD
cord-326027-58whwspe	73	8	designated	designate	VBN
cord-326027-58whwspe	73	9	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	73	10	and	and	CC
cord-326027-58whwspe	73	11	when	when	WRB
cord-326027-58whwspe	73	12	used	use	VBN
cord-326027-58whwspe	73	13	to	to	TO
cord-326027-58whwspe	73	14	infect	infect	VB
cord-326027-58whwspe	73	15	cells	cell	NNS
cord-326027-58whwspe	73	16	,	,	,
cord-326027-58whwspe	73	17	no	no	DT
cord-326027-58whwspe	73	18	trace	trace	NN
cord-326027-58whwspe	73	19	of	of	IN
cord-326027-58whwspe	73	20	the	the	DT
cord-326027-58whwspe	73	21	wild	wild	JJ
cord-326027-58whwspe	73	22	-	-	HYPH
cord-326027-58whwspe	73	23	type	type	NN
cord-326027-58whwspe	73	24	p54	p54	NN
cord-326027-58whwspe	73	25	protein	protein	NN
cord-326027-58whwspe	73	26	could	could	MD
cord-326027-58whwspe	73	27	be	be	VB
cord-326027-58whwspe	73	28	detected	detect	VBN
cord-326027-58whwspe	73	29	by	by	IN
cord-326027-58whwspe	73	30	Western	western	JJ
cord-326027-58whwspe	73	31	blot	blot	NN
cord-326027-58whwspe	73	32	in	in	IN
cord-326027-58whwspe	73	33	cell	cell	NN
cord-326027-58whwspe	73	34	extracts	extract	NNS
cord-326027-58whwspe	73	35	obtained	obtain	VBN
cord-326027-58whwspe	73	36	at	at	IN
cord-326027-58whwspe	73	37	different	different	JJ
cord-326027-58whwspe	73	38	times	time	NNS
cord-326027-58whwspe	73	39	of	of	IN
cord-326027-58whwspe	73	40	infection	infection	NN
cord-326027-58whwspe	73	41	using	use	VBG
cord-326027-58whwspe	73	42	a	a	DT
cord-326027-58whwspe	73	43	specific	specific	JJ
cord-326027-58whwspe	73	44	anti	anti	JJ
cord-326027-58whwspe	73	45	-	-	JJ
cord-326027-58whwspe	73	46	p54	p54	JJ
cord-326027-58whwspe	73	47	serum	serum	NN
cord-326027-58whwspe	73	48	(	(	-LRB-
cord-326027-58whwspe	73	49	Fig	Fig	NNP
cord-326027-58whwspe	73	50	.	.	.
cord-326027-58whwspe	73	51	3a	3a	NNP
cord-326027-58whwspe	73	52	)	)	-RRB-
cord-326027-58whwspe	73	53	.	.	.
cord-326027-58whwspe	74	1	(	(	-LRB-
cord-326027-58whwspe	74	2	a	a	LS
cord-326027-58whwspe	74	3	)	)	-RRB-
cord-326027-58whwspe	74	4	Schematic	schematic	JJ
cord-326027-58whwspe	74	5	representation	representation	NN
cord-326027-58whwspe	74	6	of	of	IN
cord-326027-58whwspe	74	7	the	the	DT
cord-326027-58whwspe	74	8	EcoRIK	ecorik	NN
cord-326027-58whwspe	74	9	and	and	CC
cord-326027-58whwspe	74	10	EcoRIE	ecorie	VB
cord-326027-58whwspe	74	11	fragments	fragment	NNS
cord-326027-58whwspe	74	12	in	in	IN
cord-326027-58whwspe	74	13	the	the	DT
cord-326027-58whwspe	74	14	genome	genome	NN
cord-326027-58whwspe	74	15	of	of	IN
cord-326027-58whwspe	74	16	the	the	DT
cord-326027-58whwspe	74	17	wild	wild	JJ
cord-326027-58whwspe	74	18	type	type	NN
cord-326027-58whwspe	74	19	ASFV	ASFV	NNP
cord-326027-58whwspe	74	20	(	(	-LRB-
cord-326027-58whwspe	74	21	BA71V	ba71v	NN
cord-326027-58whwspe	74	22	)	)	-RRB-
cord-326027-58whwspe	74	23	and	and	CC
cord-326027-58whwspe	74	24	the	the	DT
cord-326027-58whwspe	74	25	recombinants	recombinant	NNS
cord-326027-58whwspe	74	26	viruses	virus	NNS
cord-326027-58whwspe	74	27	generated	generate	VBN
cord-326027-58whwspe	74	28	(	(	-LRB-
cord-326027-58whwspe	74	29	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	74	30	and	and	CC
cord-326027-58whwspe	74	31	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	74	32	)	)	-RRB-
cord-326027-58whwspe	74	33	after	after	IN
cord-326027-58whwspe	74	34	insertion	insertion	NN
cord-326027-58whwspe	74	35	of	of	IN
cord-326027-58whwspe	74	36	chimera	chimera	NN
cord-326027-58whwspe	74	37	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	74	38	in	in	IN
cord-326027-58whwspe	74	39	Tk	Tk	NNP
cord-326027-58whwspe	74	40	locus	locus	NN
cord-326027-58whwspe	74	41	by	by	IN
cord-326027-58whwspe	74	42	homologous	homologous	JJ
cord-326027-58whwspe	74	43	recombination	recombination	NN
cord-326027-58whwspe	74	44	and	and	CC
cord-326027-58whwspe	74	45	disruption	disruption	NN
cord-326027-58whwspe	74	46	of	of	IN
cord-326027-58whwspe	74	47	the	the	DT
cord-326027-58whwspe	74	48	p54	p54	NN
cord-326027-58whwspe	74	49	gene	gene	NN
cord-326027-58whwspe	74	50	by	by	IN
cord-326027-58whwspe	74	51	insertion	insertion	NN
cord-326027-58whwspe	74	52	of	of	IN
cord-326027-58whwspe	74	53	the	the	DT
cord-326027-58whwspe	74	54	β	β	NN
cord-326027-58whwspe	74	55	-	-	HYPH
cord-326027-58whwspe	74	56	gal	gal	NN
cord-326027-58whwspe	74	57	marker	marker	NN
cord-326027-58whwspe	74	58	gene	gene	NN
cord-326027-58whwspe	74	59	,	,	,
cord-326027-58whwspe	74	60	as	as	IN
cord-326027-58whwspe	74	61	described	describe	VBN
cord-326027-58whwspe	74	62	in	in	IN
cord-326027-58whwspe	74	63	Methods	method	NNS
cord-326027-58whwspe	74	64	.	.	.
cord-326027-58whwspe	75	1	E	e	LS
cord-326027-58whwspe	75	2	:	:	:
cord-326027-58whwspe	75	3	EcoRI	ecori	NN
cord-326027-58whwspe	75	4	site	site	NN
cord-326027-58whwspe	75	5	.	.	.
cord-326027-58whwspe	76	1	(	(	-LRB-
cord-326027-58whwspe	76	2	b	b	LS
cord-326027-58whwspe	76	3	)	)	-RRB-
cord-326027-58whwspe	76	4	Genomic	genomic	JJ
cord-326027-58whwspe	76	5	analysis	analysis	NN
cord-326027-58whwspe	76	6	,	,	,
cord-326027-58whwspe	76	7	after	after	IN
cord-326027-58whwspe	76	8	EcoRI	ecori	NN
cord-326027-58whwspe	76	9	digestion	digestion	NN
cord-326027-58whwspe	76	10	,	,	,
cord-326027-58whwspe	76	11	of	of	IN
cord-326027-58whwspe	76	12	parental	parental	JJ
cord-326027-58whwspe	76	13	and	and	CC
cord-326027-58whwspe	76	14	recombinant	recombinant	JJ
cord-326027-58whwspe	76	15	ASF	asf	NN
cord-326027-58whwspe	76	16	viruses	virus	NNS
cord-326027-58whwspe	76	17	by	by	IN
cord-326027-58whwspe	76	18	Southern	southern	JJ
cord-326027-58whwspe	76	19	blot	blot	NN
cord-326027-58whwspe	76	20	using	use	VBG
cord-326027-58whwspe	76	21	a	a	DT
cord-326027-58whwspe	76	22	digoxigenin-11-dUTP	digoxigenin-11-dutp	NN
cord-326027-58whwspe	76	23	labeled	label	VBN
cord-326027-58whwspe	76	24	DNA	dna	NN
cord-326027-58whwspe	76	25	probe	probe	NN
cord-326027-58whwspe	76	26	corresponding	correspond	VBG
cord-326027-58whwspe	76	27	to	to	IN
cord-326027-58whwspe	76	28	the	the	DT
cord-326027-58whwspe	76	29	p54	p54	NN
cord-326027-58whwspe	76	30	complete	complete	JJ
cord-326027-58whwspe	76	31	coding	coding	NN
cord-326027-58whwspe	76	32	sequence	sequence	NN
cord-326027-58whwspe	76	33	.	.	.
cord-326027-58whwspe	77	1	Size	size	NN
cord-326027-58whwspe	77	2	of	of	IN
cord-326027-58whwspe	77	3	the	the	DT
cord-326027-58whwspe	77	4	fragments	fragment	NNS
cord-326027-58whwspe	77	5	probed	probe	VBN
cord-326027-58whwspe	77	6	was	be	VBD
cord-326027-58whwspe	77	7	as	as	IN
cord-326027-58whwspe	77	8	expected	expect	VBN
cord-326027-58whwspe	77	9	after	after	IN
cord-326027-58whwspe	77	10	genome	genome	NN
cord-326027-58whwspe	77	11	manipulations	manipulation	NNS
cord-326027-58whwspe	77	12	.	.	.
cord-326027-58whwspe	78	1	(	(	-LRB-
cord-326027-58whwspe	78	2	c	c	NN
cord-326027-58whwspe	78	3	and	and	CC
cord-326027-58whwspe	78	4	d	d	LS
cord-326027-58whwspe	78	5	)	)	-RRB-
cord-326027-58whwspe	79	1	After	after	IN
cord-326027-58whwspe	79	2	insertion	insertion	NN
cord-326027-58whwspe	79	3	of	of	IN
cord-326027-58whwspe	79	4	p54-EGFP	p54-egfp	JJ
cord-326027-58whwspe	79	5	recombinants	recombinant	NNS
cord-326027-58whwspe	79	6	viral	viral	JJ
cord-326027-58whwspe	79	7	plaques	plaque	NNS
cord-326027-58whwspe	79	8	formed	form	VBN
cord-326027-58whwspe	79	9	in	in	IN
cord-326027-58whwspe	79	10	Vero	Vero	NNP
cord-326027-58whwspe	79	11	cells	cell	NNS
cord-326027-58whwspe	79	12	monolayers	monolayer	NNS
cord-326027-58whwspe	79	13	exhibited	exhibit	VBD
cord-326027-58whwspe	79	14	fluorescence	fluorescence	NN
cord-326027-58whwspe	79	15	and	and	CC
cord-326027-58whwspe	79	16	allowed	allow	VBN
cord-326027-58whwspe	79	17	and	and	CC
cord-326027-58whwspe	79	18	easy	easy	JJ
cord-326027-58whwspe	79	19	detection	detection	NN
cord-326027-58whwspe	79	20	after	after	IN
cord-326027-58whwspe	79	21	5	5	CD
cord-326027-58whwspe	79	22	dpi	dpi	NN
cord-326027-58whwspe	79	23	.	.	.
cord-326027-58whwspe	80	1	(	(	-LRB-
cord-326027-58whwspe	80	2	c	c	NN
cord-326027-58whwspe	80	3	)	)	-RRB-
cord-326027-58whwspe	81	1	A	a	DT
cord-326027-58whwspe	81	2	single	single	JJ
cord-326027-58whwspe	81	3	recombinant	recombinant	JJ
cord-326027-58whwspe	81	4	fluorescent	fluorescent	NN
cord-326027-58whwspe	81	5	plaque	plaque	NN
cord-326027-58whwspe	81	6	was	be	VBD
cord-326027-58whwspe	81	7	photographed	photograph	VBN
cord-326027-58whwspe	81	8	in	in	IN
cord-326027-58whwspe	81	9	a	a	DT
cord-326027-58whwspe	81	10	fluorescence	fluorescence	NN
cord-326027-58whwspe	81	11	microscope	microscope	NN
cord-326027-58whwspe	81	12	(	(	-LRB-
cord-326027-58whwspe	81	13	original	original	JJ
cord-326027-58whwspe	81	14	magnification	magnification	NN
cord-326027-58whwspe	81	15	×100	×100	NFP
cord-326027-58whwspe	81	16	)	)	-RRB-
cord-326027-58whwspe	81	17	and	and	CC
cord-326027-58whwspe	81	18	correspondent	correspondent	NN
cord-326027-58whwspe	81	19	transmitted	transmit	VBD
cord-326027-58whwspe	81	20	light	light	JJ
cord-326027-58whwspe	81	21	image	image	NN
cord-326027-58whwspe	81	22	is	be	VBZ
cord-326027-58whwspe	81	23	shown	show	VBN
cord-326027-58whwspe	81	24	below	below	IN
cord-326027-58whwspe	81	25	(	(	-LRB-
cord-326027-58whwspe	81	26	d	d	NN
cord-326027-58whwspe	81	27	)	)	-RRB-
cord-326027-58whwspe	81	28	.	.	.
cord-326027-58whwspe	82	1	Fluorescence	fluorescence	NN
cord-326027-58whwspe	82	2	microscopy	microscopy	NN
cord-326027-58whwspe	82	3	analysis	analysis	NN
cord-326027-58whwspe	82	4	of	of	IN
cord-326027-58whwspe	82	5	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	83	1	(	(	-LRB-
cord-326027-58whwspe	83	2	e	e	NN
cord-326027-58whwspe	83	3	-	-	NN
cord-326027-58whwspe	83	4	g	g	NN
cord-326027-58whwspe	83	5	)	)	-RRB-
cord-326027-58whwspe	83	6	or	or	CC
cord-326027-58whwspe	83	7	BA71V	ba71v	VB
cord-326027-58whwspe	83	8	infected	infected	JJ
cord-326027-58whwspe	83	9	cells	cell	NNS
cord-326027-58whwspe	83	10	at	at	IN
cord-326027-58whwspe	83	11	14	14	CD
cord-326027-58whwspe	83	12	hpi	hpi	NNP
cord-326027-58whwspe	83	13	(	(	-LRB-
cord-326027-58whwspe	83	14	h	h	NNP
cord-326027-58whwspe	83	15	-	-	HYPH
cord-326027-58whwspe	83	16	j	j	NNP
cord-326027-58whwspe	83	17	)	)	-RRB-
cord-326027-58whwspe	83	18	.	.	.
cord-326027-58whwspe	84	1	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	84	2	was	be	VBD
cord-326027-58whwspe	84	3	performed	perform	VBN
cord-326027-58whwspe	84	4	by	by	IN
cord-326027-58whwspe	84	5	direct	direct	JJ
cord-326027-58whwspe	84	6	fluorescence	fluorescence	NN
cord-326027-58whwspe	84	7	(	(	-LRB-
cord-326027-58whwspe	84	8	e	e	NN
cord-326027-58whwspe	84	9	)	)	-RRB-
cord-326027-58whwspe	84	10	,	,	,
cord-326027-58whwspe	84	11	while	while	IN
cord-326027-58whwspe	84	12	p54	p54	NN
cord-326027-58whwspe	84	13	was	be	VBD
cord-326027-58whwspe	84	14	detected	detect	VBN
cord-326027-58whwspe	84	15	with	with	IN
cord-326027-58whwspe	84	16	an	an	DT
cord-326027-58whwspe	84	17	anti	anti	JJ
cord-326027-58whwspe	84	18	-	-	JJ
cord-326027-58whwspe	84	19	p54	p54	JJ
cord-326027-58whwspe	84	20	serum	serum	NN
cord-326027-58whwspe	84	21	and	and	CC
cord-326027-58whwspe	84	22	secondary	secondary	JJ
cord-326027-58whwspe	84	23	antibody	antibody	NN
cord-326027-58whwspe	84	24	conjugated	conjugate	VBN
cord-326027-58whwspe	84	25	to	to	IN
cord-326027-58whwspe	84	26	Alexa	Alexa	NNP
cord-326027-58whwspe	84	27	488	488	CD
cord-326027-58whwspe	84	28	(	(	-LRB-
cord-326027-58whwspe	84	29	h	h	NN
cord-326027-58whwspe	84	30	)	)	-RRB-
cord-326027-58whwspe	84	31	.	.	.
cord-326027-58whwspe	85	1	In	in	IN
cord-326027-58whwspe	85	2	both	both	DT
cord-326027-58whwspe	85	3	cases	case	NNS
cord-326027-58whwspe	85	4	viral	viral	JJ
cord-326027-58whwspe	85	5	and	and	CC
cord-326027-58whwspe	85	6	cellular	cellular	JJ
cord-326027-58whwspe	85	7	DNA	dna	NN
cord-326027-58whwspe	85	8	were	be	VBD
cord-326027-58whwspe	85	9	counterstained	counterstaine	VBN
cord-326027-58whwspe	85	10	with	with	IN
cord-326027-58whwspe	85	11	Hoechst	Hoechst	NNP
cord-326027-58whwspe	85	12	to	to	TO
cord-326027-58whwspe	85	13	visualize	visualize	VB
cord-326027-58whwspe	85	14	viral	viral	JJ
cord-326027-58whwspe	85	15	factories	factory	NNS
cord-326027-58whwspe	85	16	,	,	,
cord-326027-58whwspe	85	17	indicated	indicate	VBN
cord-326027-58whwspe	85	18	by	by	IN
cord-326027-58whwspe	85	19	arrows	arrow	NNS
cord-326027-58whwspe	85	20	(	(	-LRB-
cord-326027-58whwspe	85	21	f	f	NNP
cord-326027-58whwspe	85	22	and	and	CC
cord-326027-58whwspe	85	23	i	i	PRP
cord-326027-58whwspe	85	24	)	)	-RRB-
cord-326027-58whwspe	85	25	.	.	.
cord-326027-58whwspe	86	1	As	as	IN
cord-326027-58whwspe	86	2	it	-PRON-	PRP
cord-326027-58whwspe	86	3	can	can	MD
cord-326027-58whwspe	86	4	be	be	VB
cord-326027-58whwspe	86	5	observed	observe	VBN
cord-326027-58whwspe	86	6	,	,	,
cord-326027-58whwspe	86	7	in	in	IN
cord-326027-58whwspe	86	8	the	the	DT
cord-326027-58whwspe	86	9	merged	merge	VBN
cord-326027-58whwspe	86	10	images	image	NNS
cord-326027-58whwspe	86	11	(	(	-LRB-
cord-326027-58whwspe	86	12	g	g	NNP
cord-326027-58whwspe	86	13	and	and	CC
cord-326027-58whwspe	86	14	j	j	NNP
cord-326027-58whwspe	86	15	)	)	-RRB-
cord-326027-58whwspe	86	16	,	,	,
cord-326027-58whwspe	86	17	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	86	18	and	and	CC
cord-326027-58whwspe	86	19	p54	p54	CD
cord-326027-58whwspe	86	20	showed	show	VBD
cord-326027-58whwspe	86	21	similar	similar	JJ
cord-326027-58whwspe	86	22	perinuclear	perinuclear	JJ
cord-326027-58whwspe	86	23	distribution	distribution	NN
cord-326027-58whwspe	86	24	coincident	coincident	JJ
cord-326027-58whwspe	86	25	with	with	IN
cord-326027-58whwspe	86	26	viral	viral	JJ
cord-326027-58whwspe	86	27	factories	factory	NNS
cord-326027-58whwspe	86	28	.	.	.
cord-326027-58whwspe	87	1	Scale	scale	NN
cord-326027-58whwspe	87	2	bar	bar	NN
cord-326027-58whwspe	87	3	,	,	,
cord-326027-58whwspe	87	4	18	18	CD
cord-326027-58whwspe	87	5	μm	μm	NN
cord-326027-58whwspe	87	6	.	.	.
cord-326027-58whwspe	88	1	To	to	TO
cord-326027-58whwspe	88	2	determine	determine	VB
cord-326027-58whwspe	88	3	that	that	DT
cord-326027-58whwspe	88	4	protein	protein	NN
cord-326027-58whwspe	88	5	p54	p54	NN
cord-326027-58whwspe	88	6	,	,	,
cord-326027-58whwspe	88	7	a	a	DT
cord-326027-58whwspe	88	8	major	major	JJ
cord-326027-58whwspe	88	9	component	component	NN
cord-326027-58whwspe	88	10	of	of	IN
cord-326027-58whwspe	88	11	the	the	DT
cord-326027-58whwspe	88	12	external	external	JJ
cord-326027-58whwspe	88	13	envelope	envelope	NN
cord-326027-58whwspe	88	14	of	of	IN
cord-326027-58whwspe	88	15	ASFV	ASFV	NNP
cord-326027-58whwspe	88	16	,	,	,
cord-326027-58whwspe	88	17	fused	fuse	VBN
cord-326027-58whwspe	88	18	to	to	IN
cord-326027-58whwspe	88	19	EGFP	EGFP	NNP
cord-326027-58whwspe	88	20	protein	protein	NN
cord-326027-58whwspe	88	21	remains	remain	VBZ
cord-326027-58whwspe	88	22	incorporated	incorporate	VBN
cord-326027-58whwspe	88	23	to	to	IN
cord-326027-58whwspe	88	24	the	the	DT
cord-326027-58whwspe	88	25	viral	viral	JJ
cord-326027-58whwspe	88	26	particle	particle	NN
cord-326027-58whwspe	88	27	,	,	,
cord-326027-58whwspe	89	1	BA71V	BA71V	NNP
cord-326027-58whwspe	89	2	and	and	CC
cord-326027-58whwspe	89	3	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	89	4	virions	virion	NNS
cord-326027-58whwspe	89	5	were	be	VBD
cord-326027-58whwspe	89	6	Percoll	Percoll	NNP
cord-326027-58whwspe	89	7	purified	purify	VBN
cord-326027-58whwspe	89	8	and	and	CC
cord-326027-58whwspe	89	9	analyzed	analyze	VBN
cord-326027-58whwspe	89	10	by	by	IN
cord-326027-58whwspe	89	11	SDS	SDS	NNP
cord-326027-58whwspe	89	12	-	-	HYPH
cord-326027-58whwspe	89	13	PAGE	PAGE	NNP
cord-326027-58whwspe	89	14	and	and	CC
cord-326027-58whwspe	89	15	Western	western	JJ
cord-326027-58whwspe	89	16	blotting	blotting	NN
cord-326027-58whwspe	89	17	using	use	VBG
cord-326027-58whwspe	89	18	specific	specific	JJ
cord-326027-58whwspe	89	19	antibodies	antibody	NNS
cord-326027-58whwspe	89	20	(	(	-LRB-
cord-326027-58whwspe	89	21	Fig	Fig	NNP
cord-326027-58whwspe	89	22	.	.	.
cord-326027-58whwspe	89	23	2a	2a	CD
cord-326027-58whwspe	89	24	)	)	-RRB-
cord-326027-58whwspe	89	25	.	.	.
cord-326027-58whwspe	90	1	No	no	DT
cord-326027-58whwspe	90	2	reactivity	reactivity	NN
cord-326027-58whwspe	90	3	of	of	IN
cord-326027-58whwspe	90	4	anti	anti	JJ
cord-326027-58whwspe	90	5	-	-	JJ
cord-326027-58whwspe	90	6	GFP	gfp	JJ
cord-326027-58whwspe	90	7	antibody	antibody	NN
cord-326027-58whwspe	90	8	with	with	IN
cord-326027-58whwspe	90	9	BA71V	ba71v	NN
cord-326027-58whwspe	90	10	purified	purify	VBN
cord-326027-58whwspe	90	11	virus	virus	NN
cord-326027-58whwspe	90	12	was	be	VBD
cord-326027-58whwspe	90	13	found	find	VBN
cord-326027-58whwspe	90	14	,	,	,
cord-326027-58whwspe	90	15	while	while	IN
cord-326027-58whwspe	90	16	a	a	DT
cord-326027-58whwspe	90	17	51-kDa	51-kda	CD
cord-326027-58whwspe	90	18	band	band	NN
cord-326027-58whwspe	90	19	corresponding	correspond	VBG
cord-326027-58whwspe	90	20	to	to	IN
cord-326027-58whwspe	90	21	the	the	DT
cord-326027-58whwspe	90	22	expected	expect	VBN
cord-326027-58whwspe	90	23	size	size	NN
cord-326027-58whwspe	90	24	of	of	IN
cord-326027-58whwspe	90	25	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	90	26	protein	protein	NN
cord-326027-58whwspe	90	27	was	be	VBD
cord-326027-58whwspe	90	28	detected	detect	VBN
cord-326027-58whwspe	90	29	in	in	IN
cord-326027-58whwspe	90	30	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	90	31	purified	purify	VBN
cord-326027-58whwspe	90	32	virus	virus	NN
cord-326027-58whwspe	90	33	.	.	.
cord-326027-58whwspe	91	1	However	however	RB
cord-326027-58whwspe	91	2	,	,	,
cord-326027-58whwspe	91	3	when	when	WRB
cord-326027-58whwspe	91	4	an	an	DT
cord-326027-58whwspe	91	5	anti	anti	JJ
cord-326027-58whwspe	91	6	-	-	JJ
cord-326027-58whwspe	91	7	p54	p54	JJ
cord-326027-58whwspe	91	8	serum	serum	NN
cord-326027-58whwspe	91	9	was	be	VBD
cord-326027-58whwspe	91	10	probed	probe	VBN
cord-326027-58whwspe	91	11	,	,	,
cord-326027-58whwspe	91	12	a	a	DT
cord-326027-58whwspe	91	13	25-kDa	25-kda	CD
cord-326027-58whwspe	91	14	band	band	NN
cord-326027-58whwspe	91	15	appeared	appear	VBD
cord-326027-58whwspe	91	16	with	with	IN
cord-326027-58whwspe	91	17	BA71V	ba71v	NN
cord-326027-58whwspe	91	18	virus	virus	NN
cord-326027-58whwspe	91	19	and	and	CC
cord-326027-58whwspe	91	20	with	with	IN
cord-326027-58whwspe	91	21	anti	anti	JJ
cord-326027-58whwspe	91	22	-	-	JJ
cord-326027-58whwspe	91	23	GFP	gfp	JJ
cord-326027-58whwspe	91	24	serum	serum	NN
cord-326027-58whwspe	91	25	,	,	,
cord-326027-58whwspe	91	26	a	a	DT
cord-326027-58whwspe	91	27	51-kDa	51-kda	CD
cord-326027-58whwspe	91	28	band	band	NN
cord-326027-58whwspe	91	29	was	be	VBD
cord-326027-58whwspe	91	30	only	only	RB
cord-326027-58whwspe	91	31	reactive	reactive	JJ
cord-326027-58whwspe	91	32	with	with	IN
cord-326027-58whwspe	91	33	the	the	DT
cord-326027-58whwspe	91	34	recombinant	recombinant	JJ
cord-326027-58whwspe	91	35	virus	virus	NN
cord-326027-58whwspe	91	36	,	,	,
cord-326027-58whwspe	91	37	demonstrating	demonstrate	VBG
cord-326027-58whwspe	91	38	the	the	DT
cord-326027-58whwspe	91	39	incorporation	incorporation	NN
cord-326027-58whwspe	91	40	of	of	IN
cord-326027-58whwspe	91	41	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	91	42	fusion	fusion	NN
cord-326027-58whwspe	91	43	protein	protein	NN
cord-326027-58whwspe	91	44	into	into	IN
cord-326027-58whwspe	91	45	the	the	DT
cord-326027-58whwspe	91	46	virus	virus	NN
cord-326027-58whwspe	91	47	particles	particle	NNS
cord-326027-58whwspe	91	48	.	.	.
cord-326027-58whwspe	92	1	The	the	DT
cord-326027-58whwspe	92	2	typical	typical	JJ
cord-326027-58whwspe	92	3	doublet	doublet	NN
cord-326027-58whwspe	92	4	detected	detect	VBN
cord-326027-58whwspe	92	5	with	with	IN
cord-326027-58whwspe	92	6	anti	anti	JJ
cord-326027-58whwspe	92	7	-	-	JJ
cord-326027-58whwspe	92	8	p54	p54	JJ
cord-326027-58whwspe	92	9	serum	serum	NN
cord-326027-58whwspe	92	10	in	in	IN
cord-326027-58whwspe	92	11	BA71V	ba71v	NN
cord-326027-58whwspe	92	12	samples	sample	NNS
cord-326027-58whwspe	92	13	was	be	VBD
cord-326027-58whwspe	92	14	not	not	RB
cord-326027-58whwspe	92	15	present	present	JJ
cord-326027-58whwspe	92	16	in	in	IN
cord-326027-58whwspe	92	17	those	those	DT
cord-326027-58whwspe	92	18	from	from	IN
cord-326027-58whwspe	92	19	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	92	20	when	when	WRB
cord-326027-58whwspe	92	21	detection	detection	NN
cord-326027-58whwspe	92	22	of	of	IN
cord-326027-58whwspe	92	23	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	92	24	was	be	VBD
cord-326027-58whwspe	92	25	carried	carry	VBN
cord-326027-58whwspe	92	26	out	out	RP
cord-326027-58whwspe	92	27	with	with	IN
cord-326027-58whwspe	92	28	monoclonal	monoclonal	JJ
cord-326027-58whwspe	92	29	antibody	antibody	NN
cord-326027-58whwspe	92	30	anti	anti	JJ
cord-326027-58whwspe	92	31	-	-	JJ
cord-326027-58whwspe	92	32	GFP	GFP	NNP
cord-326027-58whwspe	92	33	.	.	.
cord-326027-58whwspe	93	1	It	-PRON-	PRP
cord-326027-58whwspe	93	2	is	be	VBZ
cord-326027-58whwspe	93	3	noteworthy	noteworthy	JJ
cord-326027-58whwspe	93	4	that	that	IN
cord-326027-58whwspe	93	5	the	the	DT
cord-326027-58whwspe	93	6	levels	level	NNS
cord-326027-58whwspe	93	7	of	of	IN
cord-326027-58whwspe	93	8	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	93	9	and	and	CC
cord-326027-58whwspe	93	10	p54	p54	NN
cord-326027-58whwspe	93	11	incorporated	incorporate	VBN
cord-326027-58whwspe	93	12	into	into	IN
cord-326027-58whwspe	93	13	their	-PRON-	PRP$
cord-326027-58whwspe	93	14	respective	respective	JJ
cord-326027-58whwspe	93	15	particles	particle	NNS
cord-326027-58whwspe	93	16	were	be	VBD
cord-326027-58whwspe	93	17	apparently	apparently	RB
cord-326027-58whwspe	93	18	equivalent	equivalent	JJ
cord-326027-58whwspe	93	19	,	,	,
cord-326027-58whwspe	93	20	since	since	IN
cord-326027-58whwspe	93	21	the	the	DT
cord-326027-58whwspe	93	22	amount	amount	NN
cord-326027-58whwspe	93	23	of	of	IN
cord-326027-58whwspe	93	24	total	total	JJ
cord-326027-58whwspe	93	25	virus	virus	NN
cord-326027-58whwspe	93	26	protein	protein	NN
cord-326027-58whwspe	93	27	loaded	load	VBN
cord-326027-58whwspe	93	28	in	in	IN
cord-326027-58whwspe	93	29	SDS	SDS	NNP
cord-326027-58whwspe	93	30	-	-	HYPH
cord-326027-58whwspe	93	31	PAGE	PAGE	NNP
cord-326027-58whwspe	93	32	was	be	VBD
cord-326027-58whwspe	93	33	similar	similar	JJ
cord-326027-58whwspe	93	34	in	in	IN
cord-326027-58whwspe	93	35	both	both	DT
cord-326027-58whwspe	93	36	cases	case	NNS
cord-326027-58whwspe	93	37	,	,	,
cord-326027-58whwspe	93	38	as	as	IN
cord-326027-58whwspe	93	39	judged	judge	VBN
cord-326027-58whwspe	93	40	by	by	IN
cord-326027-58whwspe	93	41	Coomasie	Coomasie	NNP
cord-326027-58whwspe	93	42	blue	blue	JJ
cord-326027-58whwspe	93	43	staining	staining	NN
cord-326027-58whwspe	93	44	.	.	.
cord-326027-58whwspe	94	1	In	in	IN
cord-326027-58whwspe	94	2	order	order	NN
cord-326027-58whwspe	94	3	to	to	TO
cord-326027-58whwspe	94	4	further	further	RB
cord-326027-58whwspe	94	5	confirm	confirm	VB
cord-326027-58whwspe	94	6	this	this	DT
cord-326027-58whwspe	94	7	,	,	,
cord-326027-58whwspe	94	8	we	-PRON-	PRP
cord-326027-58whwspe	94	9	analyzed	analyze	VBD
cord-326027-58whwspe	94	10	by	by	IN
cord-326027-58whwspe	94	11	Western	western	JJ
cord-326027-58whwspe	94	12	blotting	blot	VBG
cord-326027-58whwspe	94	13	the	the	DT
cord-326027-58whwspe	94	14	presence	presence	NN
cord-326027-58whwspe	94	15	of	of	IN
cord-326027-58whwspe	94	16	major	major	JJ
cord-326027-58whwspe	94	17	structural	structural	JJ
cord-326027-58whwspe	94	18	ASFV	ASFV	NNP
cord-326027-58whwspe	94	19	protein	protein	NN
cord-326027-58whwspe	94	20	p72	p72	NN
cord-326027-58whwspe	94	21	in	in	IN
cord-326027-58whwspe	94	22	these	these	DT
cord-326027-58whwspe	94	23	highly	highly	RB
cord-326027-58whwspe	94	24	purified	purify	VBN
cord-326027-58whwspe	94	25	virions	virion	NNS
cord-326027-58whwspe	94	26	(	(	-LRB-
cord-326027-58whwspe	94	27	Fig	Fig	NNP
cord-326027-58whwspe	94	28	.	.	.
cord-326027-58whwspe	94	29	2a	2a	CD
cord-326027-58whwspe	94	30	)	)	-RRB-
cord-326027-58whwspe	94	31	.	.	.
cord-326027-58whwspe	95	1	The	the	DT
cord-326027-58whwspe	95	2	proportion	proportion	NN
cord-326027-58whwspe	95	3	of	of	IN
cord-326027-58whwspe	95	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	95	5	relative	relative	JJ
cord-326027-58whwspe	95	6	to	to	IN
cord-326027-58whwspe	95	7	p72	p72	NN
cord-326027-58whwspe	95	8	in	in	IN
cord-326027-58whwspe	95	9	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	95	10	virions	virion	NNS
cord-326027-58whwspe	95	11	was	be	VBD
cord-326027-58whwspe	95	12	roughly	roughly	RB
cord-326027-58whwspe	95	13	the	the	DT
cord-326027-58whwspe	95	14	same	same	JJ
cord-326027-58whwspe	95	15	as	as	IN
cord-326027-58whwspe	95	16	p54	p54	CD
cord-326027-58whwspe	95	17	/	/	SYM
cord-326027-58whwspe	95	18	p72	p72	NN
cord-326027-58whwspe	95	19	rate	rate	NN
cord-326027-58whwspe	95	20	inBA71V	inba71v	NN
cord-326027-58whwspe	95	21	virions	virion	NNS
cord-326027-58whwspe	95	22	,	,	,
cord-326027-58whwspe	95	23	indicating	indicate	VBG
cord-326027-58whwspe	95	24	that	that	IN
cord-326027-58whwspe	95	25	the	the	DT
cord-326027-58whwspe	95	26	fusion	fusion	NN
cord-326027-58whwspe	95	27	protein	protein	NN
cord-326027-58whwspe	95	28	was	be	VBD
cord-326027-58whwspe	95	29	incorporated	incorporate	VBN
cord-326027-58whwspe	95	30	to	to	IN
cord-326027-58whwspe	95	31	virus	virus	NN
cord-326027-58whwspe	95	32	particle	particle	NN
cord-326027-58whwspe	95	33	at	at	IN
cord-326027-58whwspe	95	34	similar	similar	JJ
cord-326027-58whwspe	95	35	rates	rate	NNS
cord-326027-58whwspe	95	36	than	than	IN
cord-326027-58whwspe	95	37	p54	p54	ADD
cord-326027-58whwspe	95	38	.	.	.
cord-326027-58whwspe	96	1	To	to	TO
cord-326027-58whwspe	96	2	ascertain	ascertain	VB
cord-326027-58whwspe	96	3	the	the	DT
cord-326027-58whwspe	96	4	growth	growth	NN
cord-326027-58whwspe	96	5	properties	property	NNS
cord-326027-58whwspe	96	6	of	of	IN
cord-326027-58whwspe	96	7	the	the	DT
cord-326027-58whwspe	96	8	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	96	9	virus	virus	NN
cord-326027-58whwspe	96	10	,	,	,
cord-326027-58whwspe	96	11	a	a	DT
cord-326027-58whwspe	96	12	time	time	NN
cord-326027-58whwspe	96	13	course	course	NN
cord-326027-58whwspe	96	14	of	of	IN
cord-326027-58whwspe	96	15	infection	infection	NN
cord-326027-58whwspe	96	16	was	be	VBD
cord-326027-58whwspe	96	17	carried	carry	VBN
cord-326027-58whwspe	96	18	out	out	RP
cord-326027-58whwspe	96	19	for	for	IN
cord-326027-58whwspe	96	20	both	both	DT
cord-326027-58whwspe	96	21	,	,	,
cord-326027-58whwspe	96	22	wild	wild	JJ
cord-326027-58whwspe	96	23	-	-	HYPH
cord-326027-58whwspe	96	24	type	type	NN
cord-326027-58whwspe	96	25	and	and	CC
cord-326027-58whwspe	96	26	recombinant	recombinant	JJ
cord-326027-58whwspe	96	27	viruses	virus	NNS
cord-326027-58whwspe	96	28	.	.	.
cord-326027-58whwspe	97	1	Monolayers	monolayer	NNS
cord-326027-58whwspe	97	2	of	of	IN
cord-326027-58whwspe	97	3	Vero	Vero	NNP
cord-326027-58whwspe	97	4	cells	cell	NNS
cord-326027-58whwspe	97	5	were	be	VBD
cord-326027-58whwspe	97	6	infected	infect	VBN
cord-326027-58whwspe	97	7	with	with	IN
cord-326027-58whwspe	97	8	BA71V	ba71v	NN
cord-326027-58whwspe	97	9	or	or	CC
cord-326027-58whwspe	97	10	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	97	11	at	at	IN
cord-326027-58whwspe	97	12	a	a	DT
cord-326027-58whwspe	97	13	multiplicity	multiplicity	NN
cord-326027-58whwspe	97	14	of	of	IN
cord-326027-58whwspe	97	15	infection	infection	NN
cord-326027-58whwspe	97	16	(	(	-LRB-
cord-326027-58whwspe	97	17	moi	moi	NNP
cord-326027-58whwspe	97	18	)	)	-RRB-
cord-326027-58whwspe	97	19	of	of	IN
cord-326027-58whwspe	97	20	5	5	CD
cord-326027-58whwspe	97	21	pfu	pfu	NN
cord-326027-58whwspe	97	22	/	/	SYM
cord-326027-58whwspe	97	23	cell	cell	NN
cord-326027-58whwspe	97	24	and	and	CC
cord-326027-58whwspe	97	25	harvested	harvest	VBN
cord-326027-58whwspe	97	26	at	at	IN
cord-326027-58whwspe	97	27	different	different	JJ
cord-326027-58whwspe	97	28	postinfection	postinfection	NN
cord-326027-58whwspe	97	29	times	time	NNS
cord-326027-58whwspe	97	30	.	.	.
cord-326027-58whwspe	98	1	Total	total	JJ
cord-326027-58whwspe	98	2	cell	cell	NN
cord-326027-58whwspe	98	3	lysates	lysate	NNS
cord-326027-58whwspe	98	4	were	be	VBD
cord-326027-58whwspe	98	5	analyzed	analyze	VBN
cord-326027-58whwspe	98	6	by	by	IN
cord-326027-58whwspe	98	7	SDS	SDS	NNP
cord-326027-58whwspe	98	8	-	-	HYPH
cord-326027-58whwspe	98	9	PAGE	PAGE	NNP
cord-326027-58whwspe	98	10	and	and	CC
cord-326027-58whwspe	98	11	Western	western	JJ
cord-326027-58whwspe	98	12	blotting	blotting	NN
cord-326027-58whwspe	98	13	,	,	,
cord-326027-58whwspe	98	14	and	and	CC
cord-326027-58whwspe	98	15	the	the	DT
cord-326027-58whwspe	98	16	kinetics	kinetic	NNS
cord-326027-58whwspe	98	17	of	of	IN
cord-326027-58whwspe	98	18	synthesis	synthesis	NN
cord-326027-58whwspe	98	19	of	of	IN
cord-326027-58whwspe	98	20	virus	virus	NN
cord-326027-58whwspe	98	21	proteins	protein	NNS
cord-326027-58whwspe	98	22	were	be	VBD
cord-326027-58whwspe	98	23	assessed	assess	VBN
cord-326027-58whwspe	98	24	(	(	-LRB-
cord-326027-58whwspe	98	25	Fig	Fig	NNP
cord-326027-58whwspe	98	26	.	.	.
cord-326027-58whwspe	98	27	3a	3a	NNP
cord-326027-58whwspe	98	28	)	)	-RRB-
cord-326027-58whwspe	98	29	.	.	.
cord-326027-58whwspe	99	1	Western	western	JJ
cord-326027-58whwspe	99	2	blotting	blot	VBG
cord-326027-58whwspe	99	3	with	with	IN
cord-326027-58whwspe	99	4	an	an	DT
cord-326027-58whwspe	99	5	anti	anti	JJ
cord-326027-58whwspe	99	6	-	-	JJ
cord-326027-58whwspe	99	7	p54	p54	JJ
cord-326027-58whwspe	99	8	serum	serum	NN
cord-326027-58whwspe	99	9	allowed	allow	VBD
cord-326027-58whwspe	99	10	the	the	DT
cord-326027-58whwspe	99	11	identification	identification	NN
cord-326027-58whwspe	99	12	of	of	IN
cord-326027-58whwspe	99	13	a	a	DT
cord-326027-58whwspe	99	14	51-kDa	51-kda	CD
cord-326027-58whwspe	99	15	band	band	NN
cord-326027-58whwspe	99	16	,	,	,
cord-326027-58whwspe	99	17	the	the	DT
cord-326027-58whwspe	99	18	expected	expect	VBN
cord-326027-58whwspe	99	19	size	size	NN
cord-326027-58whwspe	99	20	for	for	IN
cord-326027-58whwspe	99	21	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	99	22	,	,	,
cord-326027-58whwspe	99	23	only	only	RB
cord-326027-58whwspe	99	24	in	in	IN
cord-326027-58whwspe	99	25	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	99	26	infected	infected	JJ
cord-326027-58whwspe	99	27	cell	cell	NN
cord-326027-58whwspe	99	28	extracts	extract	NNS
cord-326027-58whwspe	99	29	from	from	IN
cord-326027-58whwspe	99	30	8	8	CD
cord-326027-58whwspe	99	31	to	to	TO
cord-326027-58whwspe	99	32	24	24	CD
cord-326027-58whwspe	99	33	hpi	hpi	NNS
cord-326027-58whwspe	99	34	.	.	.
cord-326027-58whwspe	100	1	At	at	IN
cord-326027-58whwspe	100	2	the	the	DT
cord-326027-58whwspe	100	3	same	same	JJ
cord-326027-58whwspe	100	4	times	time	NNS
cord-326027-58whwspe	100	5	postinfection	postinfection	NN
cord-326027-58whwspe	100	6	,	,	,
cord-326027-58whwspe	100	7	p54	p54	NNP
cord-326027-58whwspe	100	8	was	be	VBD
cord-326027-58whwspe	100	9	detected	detect	VBN
cord-326027-58whwspe	100	10	as	as	IN
cord-326027-58whwspe	100	11	a	a	DT
cord-326027-58whwspe	100	12	doublet	doublet	NN
cord-326027-58whwspe	100	13	in	in	IN
cord-326027-58whwspe	100	14	BA71V	ba71v	NN
cord-326027-58whwspe	100	15	infected	infected	JJ
cord-326027-58whwspe	100	16	cell	cell	NN
cord-326027-58whwspe	100	17	extracts	extract	NNS
cord-326027-58whwspe	100	18	while	while	IN
cord-326027-58whwspe	100	19	a	a	DT
cord-326027-58whwspe	100	20	single	single	JJ
cord-326027-58whwspe	100	21	51	51	CD
cord-326027-58whwspe	100	22	kDa	kda	NN
cord-326027-58whwspe	100	23	band	band	NN
cord-326027-58whwspe	100	24	was	be	VBD
cord-326027-58whwspe	100	25	detected	detect	VBN
cord-326027-58whwspe	100	26	with	with	IN
cord-326027-58whwspe	100	27	a	a	DT
cord-326027-58whwspe	100	28	monoclonal	monoclonal	JJ
cord-326027-58whwspe	100	29	antibody	antibody	NN
cord-326027-58whwspe	100	30	against	against	IN
cord-326027-58whwspe	100	31	GFP	GFP	NNP
cord-326027-58whwspe	100	32	,	,	,
cord-326027-58whwspe	100	33	only	only	RB
cord-326027-58whwspe	100	34	in	in	IN
cord-326027-58whwspe	100	35	recombinant	recombinant	JJ
cord-326027-58whwspe	100	36	virus	virus	NN
cord-326027-58whwspe	100	37	infected	infect	VBN
cord-326027-58whwspe	100	38	cell	cell	NN
cord-326027-58whwspe	100	39	extracts	extract	NNS
cord-326027-58whwspe	100	40	at	at	IN
cord-326027-58whwspe	100	41	these	these	DT
cord-326027-58whwspe	100	42	time	time	NN
cord-326027-58whwspe	100	43	points	point	NNS
cord-326027-58whwspe	100	44	.	.	.
cord-326027-58whwspe	101	1	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	101	2	apparently	apparently	RB
cord-326027-58whwspe	101	3	remained	remain	VBD
cord-326027-58whwspe	101	4	intact	intact	JJ
cord-326027-58whwspe	101	5	throughout	throughout	IN
cord-326027-58whwspe	101	6	the	the	DT
cord-326027-58whwspe	101	7	course	course	NN
cord-326027-58whwspe	101	8	of	of	IN
cord-326027-58whwspe	101	9	the	the	DT
cord-326027-58whwspe	101	10	infection	infection	NN
cord-326027-58whwspe	101	11	and	and	CC
cord-326027-58whwspe	101	12	sample	sample	NN
cord-326027-58whwspe	101	13	preparation	preparation	NN
cord-326027-58whwspe	101	14	,	,	,
cord-326027-58whwspe	101	15	since	since	IN
cord-326027-58whwspe	101	16	no	no	DT
cord-326027-58whwspe	101	17	doublet	doublet	NN
cord-326027-58whwspe	101	18	was	be	VBD
cord-326027-58whwspe	101	19	observed	observe	VBN
cord-326027-58whwspe	101	20	in	in	IN
cord-326027-58whwspe	101	21	WB	WB	NNP
cord-326027-58whwspe	101	22	.	.	.
cord-326027-58whwspe	102	1	As	as	IN
cord-326027-58whwspe	102	2	expected	expect	VBN
cord-326027-58whwspe	102	3	,	,	,
cord-326027-58whwspe	102	4	fusion	fusion	NN
cord-326027-58whwspe	102	5	protein	protein	NN
cord-326027-58whwspe	102	6	was	be	VBD
cord-326027-58whwspe	102	7	synthesized	synthesize	VBN
cord-326027-58whwspe	102	8	late	late	RB
cord-326027-58whwspe	102	9	after	after	IN
cord-326027-58whwspe	102	10	infection	infection	NN
cord-326027-58whwspe	102	11	(	(	-LRB-
cord-326027-58whwspe	102	12	detectable	detectable	JJ
cord-326027-58whwspe	102	13	from	from	IN
cord-326027-58whwspe	102	14	8	8	CD
cord-326027-58whwspe	102	15	hpi	hpi	NNS
cord-326027-58whwspe	102	16	by	by	IN
cord-326027-58whwspe	102	17	WB	WB	NNP
cord-326027-58whwspe	102	18	)	)	-RRB-
cord-326027-58whwspe	102	19	,	,	,
cord-326027-58whwspe	102	20	in	in	IN
cord-326027-58whwspe	102	21	contrast	contrast	NN
cord-326027-58whwspe	102	22	to	to	IN
cord-326027-58whwspe	102	23	the	the	DT
cord-326027-58whwspe	102	24	early	early	JJ
cord-326027-58whwspe	102	25	protein	protein	NN
cord-326027-58whwspe	102	26	p30	p30	NN
cord-326027-58whwspe	102	27	used	use	VBD
cord-326027-58whwspe	102	28	as	as	IN
cord-326027-58whwspe	102	29	a	a	DT
cord-326027-58whwspe	102	30	control	control	NN
cord-326027-58whwspe	102	31	,	,	,
cord-326027-58whwspe	102	32	which	which	WDT
cord-326027-58whwspe	102	33	was	be	VBD
cord-326027-58whwspe	102	34	detected	detect	VBN
cord-326027-58whwspe	102	35	from	from	IN
cord-326027-58whwspe	102	36	4	4	CD
cord-326027-58whwspe	102	37	hpi	hpi	NNP
cord-326027-58whwspe	102	38	.	.	.
cord-326027-58whwspe	103	1	To	to	TO
cord-326027-58whwspe	103	2	demonstrate	demonstrate	VB
cord-326027-58whwspe	103	3	that	that	IN
cord-326027-58whwspe	103	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	103	5	is	be	VBZ
cord-326027-58whwspe	103	6	synthesized	synthesize	VBN
cord-326027-58whwspe	103	7	during	during	IN
cord-326027-58whwspe	103	8	the	the	DT
cord-326027-58whwspe	103	9	late	late	JJ
cord-326027-58whwspe	103	10	viral	viral	JJ
cord-326027-58whwspe	103	11	expression	expression	NN
cord-326027-58whwspe	103	12	phase	phase	NN
cord-326027-58whwspe	103	13	under	under	IN
cord-326027-58whwspe	103	14	the	the	DT
cord-326027-58whwspe	103	15	control	control	NN
cord-326027-58whwspe	103	16	of	of	IN
cord-326027-58whwspe	103	17	p54	p54	NN
cord-326027-58whwspe	103	18	promoter	promoter	NN
cord-326027-58whwspe	103	19	,	,	,
cord-326027-58whwspe	103	20	we	-PRON-	PRP
cord-326027-58whwspe	103	21	examined	examine	VBD
cord-326027-58whwspe	103	22	the	the	DT
cord-326027-58whwspe	103	23	synthesis	synthesis	NN
cord-326027-58whwspe	103	24	of	of	IN
cord-326027-58whwspe	103	25	fusion	fusion	NN
cord-326027-58whwspe	103	26	protein	protein	NN
cord-326027-58whwspe	103	27	in	in	IN
cord-326027-58whwspe	103	28	the	the	DT
cord-326027-58whwspe	103	29	presence	presence	NN
cord-326027-58whwspe	103	30	and	and	CC
cord-326027-58whwspe	103	31	absence	absence	NN
cord-326027-58whwspe	103	32	of	of	IN
cord-326027-58whwspe	103	33	a	a	DT
cord-326027-58whwspe	103	34	DNA	dna	NN
cord-326027-58whwspe	103	35	replication	replication	NN
cord-326027-58whwspe	103	36	inhibitor	inhibitor	NN
cord-326027-58whwspe	103	37	,	,	,
cord-326027-58whwspe	103	38	such	such	JJ
cord-326027-58whwspe	103	39	as	as	IN
cord-326027-58whwspe	103	40	cytosine	cytosine	NN
cord-326027-58whwspe	103	41	βarabinofuranoside	βarabinofuranoside	NN
cord-326027-58whwspe	103	42	(	(	-LRB-
cord-326027-58whwspe	103	43	Ara	Ara	NNP
cord-326027-58whwspe	103	44	-	-	HYPH
cord-326027-58whwspe	103	45	C	C	NNP
cord-326027-58whwspe	103	46	)	)	-RRB-
cord-326027-58whwspe	103	47	.	.	.
cord-326027-58whwspe	104	1	In	in	IN
cord-326027-58whwspe	104	2	the	the	DT
cord-326027-58whwspe	104	3	presence	presence	NN
cord-326027-58whwspe	104	4	of	of	IN
cord-326027-58whwspe	104	5	Ara	Ara	NNP
cord-326027-58whwspe	104	6	-	-	HYPH
cord-326027-58whwspe	104	7	C	C	NNP
cord-326027-58whwspe	104	8	,	,	,
cord-326027-58whwspe	104	9	we	-PRON-	PRP
cord-326027-58whwspe	104	10	could	could	MD
cord-326027-58whwspe	104	11	detect	detect	VB
cord-326027-58whwspe	104	12	neither	neither	CC
cord-326027-58whwspe	104	13	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	104	14	nor	nor	CC
cord-326027-58whwspe	104	15	p54	p54	VBG
cord-326027-58whwspe	104	16	with	with	IN
cord-326027-58whwspe	104	17	anti	anti	JJ
cord-326027-58whwspe	104	18	-	-	JJ
cord-326027-58whwspe	104	19	p54	p54	JJ
cord-326027-58whwspe	104	20	serum	serum	NN
cord-326027-58whwspe	104	21	,	,	,
cord-326027-58whwspe	104	22	while	while	IN
cord-326027-58whwspe	104	23	early	early	RB
cord-326027-58whwspe	104	24	ASFV	ASFV	NNP
cord-326027-58whwspe	104	25	protein	protein	NN
cord-326027-58whwspe	104	26	p30	p30	NNP
cord-326027-58whwspe	104	27	expression	expression	NN
cord-326027-58whwspe	104	28	was	be	VBD
cord-326027-58whwspe	104	29	confirmed	confirm	VBN
cord-326027-58whwspe	104	30	by	by	IN
cord-326027-58whwspe	104	31	WB	WB	NNP
cord-326027-58whwspe	104	32	with	with	IN
cord-326027-58whwspe	104	33	a	a	DT
cord-326027-58whwspe	104	34	monoclonal	monoclonal	JJ
cord-326027-58whwspe	104	35	antibody	antibody	NN
cord-326027-58whwspe	104	36	against	against	IN
cord-326027-58whwspe	104	37	p30	p30	NNP
cord-326027-58whwspe	104	38	in	in	IN
cord-326027-58whwspe	104	39	the	the	DT
cord-326027-58whwspe	104	40	presence	presence	NN
cord-326027-58whwspe	104	41	and	and	CC
cord-326027-58whwspe	104	42	absence	absence	NN
cord-326027-58whwspe	104	43	of	of	IN
cord-326027-58whwspe	104	44	inhibitor	inhibitor	NN
cord-326027-58whwspe	104	45	(	(	-LRB-
cord-326027-58whwspe	104	46	Fig	Fig	NNP
cord-326027-58whwspe	104	47	.	.	.
cord-326027-58whwspe	104	48	3b	3b	CD
cord-326027-58whwspe	104	49	)	)	-RRB-
cord-326027-58whwspe	104	50	.	.	.
cord-326027-58whwspe	105	1	These	these	DT
cord-326027-58whwspe	105	2	results	result	NNS
cord-326027-58whwspe	105	3	demonstrated	demonstrate	VBD
cord-326027-58whwspe	105	4	that	that	IN
cord-326027-58whwspe	105	5	virus	virus	NN
cord-326027-58whwspe	105	6	gene	gene	NN
cord-326027-58whwspe	105	7	expression	expression	NN
cord-326027-58whwspe	105	8	was	be	VBD
cord-326027-58whwspe	105	9	similar	similar	JJ
cord-326027-58whwspe	105	10	in	in	IN
cord-326027-58whwspe	105	11	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	105	12	and	and	CC
cord-326027-58whwspe	105	13	wild	wild	JJ
cord-326027-58whwspe	105	14	-	-	HYPH
cord-326027-58whwspe	105	15	type	type	NN
cord-326027-58whwspe	105	16	virus	virus	NN
cord-326027-58whwspe	105	17	infected	infect	VBN
cord-326027-58whwspe	105	18	cells	cell	NNS
cord-326027-58whwspe	105	19	,	,	,
cord-326027-58whwspe	105	20	but	but	CC
cord-326027-58whwspe	105	21	it	-PRON-	PRP
cord-326027-58whwspe	105	22	was	be	VBD
cord-326027-58whwspe	105	23	conceivable	conceivable	JJ
cord-326027-58whwspe	105	24	,	,	,
cord-326027-58whwspe	105	25	since	since	IN
cord-326027-58whwspe	105	26	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	105	27	is	be	VBZ
cord-326027-58whwspe	105	28	a	a	DT
cord-326027-58whwspe	105	29	structural	structural	JJ
cord-326027-58whwspe	105	30	component	component	NN
cord-326027-58whwspe	105	31	of	of	IN
cord-326027-58whwspe	105	32	the	the	DT
cord-326027-58whwspe	105	33	virus	virus	NN
cord-326027-58whwspe	105	34	particle	particle	NN
cord-326027-58whwspe	105	35	,	,	,
cord-326027-58whwspe	105	36	that	that	DT
cord-326027-58whwspe	105	37	assembly	assembly	NN
cord-326027-58whwspe	106	1	and/or	and/or	CC
cord-326027-58whwspe	106	2	release	release	VB
cord-326027-58whwspe	106	3	from	from	IN
cord-326027-58whwspe	106	4	the	the	DT
cord-326027-58whwspe	106	5	cell	cell	NN
cord-326027-58whwspe	106	6	of	of	IN
cord-326027-58whwspe	106	7	the	the	DT
cord-326027-58whwspe	106	8	double	double	JJ
cord-326027-58whwspe	106	9	recombinant	recombinant	JJ
cord-326027-58whwspe	106	10	virus	virus	NN
cord-326027-58whwspe	106	11	could	could	MD
cord-326027-58whwspe	106	12	be	be	VB
cord-326027-58whwspe	106	13	in	in	IN
cord-326027-58whwspe	106	14	some	some	DT
cord-326027-58whwspe	106	15	way	way	NN
cord-326027-58whwspe	106	16	restricted	restricted	JJ
cord-326027-58whwspe	106	17	.	.	.
cord-326027-58whwspe	107	1	To	to	TO
cord-326027-58whwspe	107	2	assess	assess	VB
cord-326027-58whwspe	107	3	the	the	DT
cord-326027-58whwspe	107	4	rate	rate	NN
cord-326027-58whwspe	107	5	of	of	IN
cord-326027-58whwspe	107	6	virus	virus	NN
cord-326027-58whwspe	107	7	assembly	assembly	NN
cord-326027-58whwspe	107	8	and	and	CC
cord-326027-58whwspe	107	9	egress	egress	NN
cord-326027-58whwspe	107	10	throughout	throughout	IN
cord-326027-58whwspe	107	11	several	several	JJ
cord-326027-58whwspe	107	12	infection	infection	NN
cord-326027-58whwspe	107	13	cell	cell	NN
cord-326027-58whwspe	107	14	cycles	cycle	NNS
cord-326027-58whwspe	107	15	,	,	,
cord-326027-58whwspe	107	16	growth	growth	NN
cord-326027-58whwspe	107	17	curves	curve	NNS
cord-326027-58whwspe	107	18	were	be	VBD
cord-326027-58whwspe	107	19	carried	carry	VBN
cord-326027-58whwspe	107	20	out	out	RP
cord-326027-58whwspe	107	21	for	for	IN
cord-326027-58whwspe	107	22	both	both	CC
cord-326027-58whwspe	107	23	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	107	24	and	and	CC
cord-326027-58whwspe	107	25	the	the	DT
cord-326027-58whwspe	107	26	BA71V	ba71v	NN
cord-326027-58whwspe	107	27	virus	virus	NN
cord-326027-58whwspe	107	28	.	.	.
cord-326027-58whwspe	108	1	Vero	Vero	NNP
cord-326027-58whwspe	108	2	cells	cell	NNS
cord-326027-58whwspe	108	3	were	be	VBD
cord-326027-58whwspe	108	4	infected	infect	VBN
cord-326027-58whwspe	108	5	at	at	IN
cord-326027-58whwspe	108	6	a	a	DT
cord-326027-58whwspe	108	7	multiplicity	multiplicity	NN
cord-326027-58whwspe	108	8	of	of	IN
cord-326027-58whwspe	108	9	0.1	0.1	CD
cord-326027-58whwspe	108	10	pfu	pfu	NN
cord-326027-58whwspe	108	11	/	/	SYM
cord-326027-58whwspe	108	12	cell	cell	NN
cord-326027-58whwspe	108	13	and	and	CC
cord-326027-58whwspe	108	14	harvested	harvest	VBD
cord-326027-58whwspe	108	15	every	every	DT
cord-326027-58whwspe	108	16	10	10	CD
cord-326027-58whwspe	108	17	h	h	NN
cord-326027-58whwspe	108	18	for	for	IN
cord-326027-58whwspe	108	19	72	72	CD
cord-326027-58whwspe	108	20	h	h	NN
cord-326027-58whwspe	108	21	,	,	,
cord-326027-58whwspe	108	22	and	and	CC
cord-326027-58whwspe	108	23	both	both	CC
cord-326027-58whwspe	108	24	extracellular	extracellular	JJ
cord-326027-58whwspe	108	25	and	and	CC
cord-326027-58whwspe	108	26	intracellular	intracellular	JJ
cord-326027-58whwspe	108	27	virus	virus	NN
cord-326027-58whwspe	108	28	yields	yield	NNS
cord-326027-58whwspe	108	29	were	be	VBD
cord-326027-58whwspe	108	30	calculated	calculate	VBN
cord-326027-58whwspe	108	31	for	for	IN
cord-326027-58whwspe	108	32	both	both	DT
cord-326027-58whwspe	108	33	viruses	virus	NNS
cord-326027-58whwspe	108	34	.	.	.
cord-326027-58whwspe	109	1	The	the	DT
cord-326027-58whwspe	109	2	results	result	NNS
cord-326027-58whwspe	109	3	showed	show	VBD
cord-326027-58whwspe	109	4	that	that	IN
cord-326027-58whwspe	109	5	the	the	DT
cord-326027-58whwspe	109	6	growth	growth	NN
cord-326027-58whwspe	109	7	curves	curve	NNS
cord-326027-58whwspe	109	8	for	for	IN
cord-326027-58whwspe	109	9	extracellular	extracellular	JJ
cord-326027-58whwspe	109	10	and	and	CC
cord-326027-58whwspe	109	11	intracellular	intracellular	JJ
cord-326027-58whwspe	109	12	virus	virus	NN
cord-326027-58whwspe	109	13	were	be	VBD
cord-326027-58whwspe	109	14	similar	similar	JJ
cord-326027-58whwspe	109	15	for	for	IN
cord-326027-58whwspe	109	16	wild	wild	JJ
cord-326027-58whwspe	109	17	-	-	HYPH
cord-326027-58whwspe	109	18	type	type	NN
cord-326027-58whwspe	109	19	and	and	CC
cord-326027-58whwspe	109	20	recombinant	recombinant	JJ
cord-326027-58whwspe	109	21	virus	virus	NN
cord-326027-58whwspe	109	22	infections	infection	NNS
cord-326027-58whwspe	109	23	,	,	,
cord-326027-58whwspe	109	24	not	not	RB
cord-326027-58whwspe	109	25	only	only	RB
cord-326027-58whwspe	109	26	in	in	IN
cord-326027-58whwspe	109	27	total	total	JJ
cord-326027-58whwspe	109	28	infectious	infectious	JJ
cord-326027-58whwspe	109	29	virus	virus	NN
cord-326027-58whwspe	109	30	production	production	NN
cord-326027-58whwspe	109	31	after	after	IN
cord-326027-58whwspe	109	32	72	72	CD
cord-326027-58whwspe	109	33	hpi	hpi	NN
cord-326027-58whwspe	109	34	,	,	,
cord-326027-58whwspe	109	35	but	but	CC
cord-326027-58whwspe	109	36	at	at	IN
cord-326027-58whwspe	109	37	every	every	DT
cord-326027-58whwspe	109	38	time	time	NN
cord-326027-58whwspe	109	39	point	point	NN
cord-326027-58whwspe	109	40	we	-PRON-	PRP
cord-326027-58whwspe	109	41	examined	examine	VBD
cord-326027-58whwspe	109	42	(	(	-LRB-
cord-326027-58whwspe	109	43	Fig	Fig	NNP
cord-326027-58whwspe	109	44	.	.	.
cord-326027-58whwspe	109	45	3c	3c	NNP
cord-326027-58whwspe	109	46	)	)	-RRB-
cord-326027-58whwspe	109	47	.	.	.
cord-326027-58whwspe	110	1	These	these	DT
cord-326027-58whwspe	110	2	results	result	NNS
cord-326027-58whwspe	110	3	imply	imply	VBP
cord-326027-58whwspe	110	4	that	that	IN
cord-326027-58whwspe	110	5	the	the	DT
cord-326027-58whwspe	110	6	rates	rate	NNS
cord-326027-58whwspe	110	7	of	of	IN
cord-326027-58whwspe	110	8	both	both	DT
cord-326027-58whwspe	110	9	virus	virus	NN
cord-326027-58whwspe	110	10	assembly	assembly	NN
cord-326027-58whwspe	110	11	and	and	CC
cord-326027-58whwspe	110	12	virus	virus	NN
cord-326027-58whwspe	110	13	egress	egress	NN
cord-326027-58whwspe	110	14	from	from	IN
cord-326027-58whwspe	110	15	the	the	DT
cord-326027-58whwspe	110	16	cell	cell	NN
cord-326027-58whwspe	110	17	were	be	VBD
cord-326027-58whwspe	110	18	the	the	DT
cord-326027-58whwspe	110	19	same	same	JJ
cord-326027-58whwspe	110	20	for	for	IN
cord-326027-58whwspe	110	21	the	the	DT
cord-326027-58whwspe	110	22	two	two	CD
cord-326027-58whwspe	110	23	viruses	virus	NNS
cord-326027-58whwspe	110	24	.	.	.
cord-326027-58whwspe	111	1	Cell	cell	NN
cord-326027-58whwspe	111	2	monolayers	monolayer	NNS
cord-326027-58whwspe	111	3	were	be	VBD
cord-326027-58whwspe	111	4	infected	infect	VBN
cord-326027-58whwspe	111	5	with	with	IN
cord-326027-58whwspe	111	6	BA71V	ba71v	NN
cord-326027-58whwspe	111	7	or	or	CC
cord-326027-58whwspe	111	8	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	111	9	at	at	IN
cord-326027-58whwspe	111	10	5	5	CD
cord-326027-58whwspe	111	11	pfu/	pfu/	CD
cord-326027-58whwspe	111	12	,	,	,
cord-326027-58whwspe	111	13	in	in	IN
cord-326027-58whwspe	111	14	presence	presence	NN
cord-326027-58whwspe	111	15	(	(	-LRB-
cord-326027-58whwspe	111	16	+	+	NN
cord-326027-58whwspe	111	17	)	)	-RRB-
cord-326027-58whwspe	111	18	or	or	CC
cord-326027-58whwspe	111	19	absence	absence	NN
cord-326027-58whwspe	111	20	(	(	-LRB-
cord-326027-58whwspe	111	21	−	−	NN
cord-326027-58whwspe	111	22	)	)	-RRB-
cord-326027-58whwspe	111	23	of	of	IN
cord-326027-58whwspe	111	24	inhibitor	inhibitor	NN
cord-326027-58whwspe	111	25	cytosine	cytosine	NN
cord-326027-58whwspe	111	26	βarabinofuranoside	βarabinofuranoside	NN
cord-326027-58whwspe	111	27	(	(	-LRB-
cord-326027-58whwspe	111	28	Ara	Ara	NNP
cord-326027-58whwspe	111	29	-	-	HYPH
cord-326027-58whwspe	111	30	C	C	NNP
cord-326027-58whwspe	111	31	)	)	-RRB-
cord-326027-58whwspe	111	32	to	to	TO
cord-326027-58whwspe	111	33	discriminate	discriminate	VB
cord-326027-58whwspe	111	34	late	late	RB
cord-326027-58whwspe	111	35	from	from	IN
cord-326027-58whwspe	111	36	early	early	JJ
cord-326027-58whwspe	111	37	viral	viral	JJ
cord-326027-58whwspe	111	38	protein	protein	NN
cord-326027-58whwspe	111	39	synthesis	synthesis	NN
cord-326027-58whwspe	111	40	.	.	.
cord-326027-58whwspe	112	1	Cells	cell	NNS
cord-326027-58whwspe	112	2	were	be	VBD
cord-326027-58whwspe	112	3	harvested	harvest	VBN
cord-326027-58whwspe	112	4	at	at	IN
cord-326027-58whwspe	112	5	14	14	CD
cord-326027-58whwspe	112	6	hpi	hpi	NNS
cord-326027-58whwspe	112	7	and	and	CC
cord-326027-58whwspe	112	8	analyzed	analyze	VBN
cord-326027-58whwspe	112	9	by	by	IN
cord-326027-58whwspe	112	10	Western	western	JJ
cord-326027-58whwspe	112	11	blot	blot	NN
cord-326027-58whwspe	112	12	with	with	IN
cord-326027-58whwspe	112	13	a	a	DT
cord-326027-58whwspe	112	14	serum	serum	NN
cord-326027-58whwspe	112	15	anti	anti	NN
cord-326027-58whwspe	112	16	-	-	JJ
cord-326027-58whwspe	112	17	p54	p54	JJ
cord-326027-58whwspe	112	18	that	that	WDT
cord-326027-58whwspe	112	19	recognized	recognize	VBD
cord-326027-58whwspe	112	20	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	112	21	and	and	CC
cord-326027-58whwspe	112	22	p54	p54	VBG
cord-326027-58whwspe	112	23	only	only	RB
cord-326027-58whwspe	112	24	in	in	IN
cord-326027-58whwspe	112	25	absence	absence	NN
cord-326027-58whwspe	112	26	of	of	IN
cord-326027-58whwspe	112	27	Ara	Ara	NNP
cord-326027-58whwspe	112	28	-	-	HYPH
cord-326027-58whwspe	112	29	C.	C.	NNP
cord-326027-58whwspe	112	30	Early	Early	NNP
cord-326027-58whwspe	112	31	ASFV	ASFV	NNP
cord-326027-58whwspe	112	32	protein	protein	NN
cord-326027-58whwspe	112	33	p30	p30	NN
cord-326027-58whwspe	112	34	was	be	VBD
cord-326027-58whwspe	112	35	detected	detect	VBN
cord-326027-58whwspe	112	36	in	in	IN
cord-326027-58whwspe	112	37	presence	presence	NN
cord-326027-58whwspe	112	38	and	and	CC
cord-326027-58whwspe	112	39	absence	absence	NN
cord-326027-58whwspe	112	40	of	of	IN
cord-326027-58whwspe	112	41	inhibitor	inhibitor	NN
cord-326027-58whwspe	112	42	using	use	VBG
cord-326027-58whwspe	112	43	monoclonal	monoclonal	JJ
cord-326027-58whwspe	112	44	antibody	antibody	NN
cord-326027-58whwspe	112	45	anti	anti	NNP
cord-326027-58whwspe	112	46	-	-	NN
cord-326027-58whwspe	112	47	p30	p30	NNP
cord-326027-58whwspe	112	48	.	.	.
cord-326027-58whwspe	113	1	(	(	-LRB-
cord-326027-58whwspe	113	2	c	c	NN
cord-326027-58whwspe	113	3	)	)	-RRB-
cord-326027-58whwspe	113	4	Analysis	analysis	NN
cord-326027-58whwspe	113	5	of	of	IN
cord-326027-58whwspe	113	6	the	the	DT
cord-326027-58whwspe	113	7	replicative	replicative	JJ
cord-326027-58whwspe	113	8	phenotype	phenotype	NN
cord-326027-58whwspe	113	9	of	of	IN
cord-326027-58whwspe	113	10	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	113	11	.	.	.
cord-326027-58whwspe	114	1	Cells	cell	NNS
cord-326027-58whwspe	114	2	were	be	VBD
cord-326027-58whwspe	114	3	infected	infect	VBN
cord-326027-58whwspe	114	4	with	with	IN
cord-326027-58whwspe	114	5	0.1	0.1	CD
cord-326027-58whwspe	114	6	pfu	pfu	NN
cord-326027-58whwspe	114	7	/	/	SYM
cord-326027-58whwspe	114	8	cell	cell	NN
cord-326027-58whwspe	114	9	of	of	IN
cord-326027-58whwspe	114	10	BA71V	ba71v	NN
cord-326027-58whwspe	114	11	or	or	CC
cord-326027-58whwspe	114	12	B54GFP	B54GFP	NNP
cord-326027-58whwspe	114	13	.	.	.
cord-326027-58whwspe	115	1	Every	every	DT
cord-326027-58whwspe	115	2	12	12	CD
cord-326027-58whwspe	115	3	hpi	hpi	NNP
cord-326027-58whwspe	115	4	(	(	-LRB-
cord-326027-58whwspe	115	5	for	for	IN
cord-326027-58whwspe	115	6	a	a	DT
cord-326027-58whwspe	115	7	maximum	maximum	NN
cord-326027-58whwspe	115	8	of	of	IN
cord-326027-58whwspe	115	9	72	72	CD
cord-326027-58whwspe	115	10	)	)	-RRB-
cord-326027-58whwspe	115	11	extracellular	extracellular	JJ
cord-326027-58whwspe	115	12	and	and	CC
cord-326027-58whwspe	115	13	intracellular	intracellular	JJ
cord-326027-58whwspe	115	14	infectious	infectious	JJ
cord-326027-58whwspe	115	15	virus	virus	NN
cord-326027-58whwspe	115	16	progeny	progeny	NN
cord-326027-58whwspe	115	17	was	be	VBD
cord-326027-58whwspe	115	18	estimated	estimate	VBN
cord-326027-58whwspe	115	19	by	by	IN
cord-326027-58whwspe	115	20	plaque	plaque	NN
cord-326027-58whwspe	115	21	assays	assay	NNS
cord-326027-58whwspe	115	22	.	.	.
cord-326027-58whwspe	116	1	Growth	growth	NN
cord-326027-58whwspe	116	2	curve	curve	NN
cord-326027-58whwspe	116	3	obtained	obtain	VBN
cord-326027-58whwspe	116	4	for	for	IN
cord-326027-58whwspe	116	5	recombinant	recombinant	JJ
cord-326027-58whwspe	116	6	virus	virus	NN
cord-326027-58whwspe	116	7	resulted	result	VBD
cord-326027-58whwspe	116	8	similar	similar	JJ
cord-326027-58whwspe	116	9	to	to	IN
cord-326027-58whwspe	116	10	the	the	DT
cord-326027-58whwspe	116	11	parental	parental	JJ
cord-326027-58whwspe	116	12	virus	virus	NN
cord-326027-58whwspe	117	1	BA71V.	BA71V.	NNP
cord-326027-58whwspe	118	1	The	the	DT
cord-326027-58whwspe	118	2	incorporation	incorporation	NN
cord-326027-58whwspe	118	3	of	of	IN
cord-326027-58whwspe	118	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	118	5	into	into	IN
cord-326027-58whwspe	118	6	virions	virion	NNS
cord-326027-58whwspe	118	7	of	of	IN
cord-326027-58whwspe	118	8	the	the	DT
cord-326027-58whwspe	118	9	ASFV	ASFV	NNP
cord-326027-58whwspe	118	10	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	118	11	makes	make	VBZ
cord-326027-58whwspe	118	12	possible	possible	JJ
cord-326027-58whwspe	118	13	that	that	IN
cord-326027-58whwspe	118	14	these	these	DT
cord-326027-58whwspe	118	15	particles	particle	NNS
cord-326027-58whwspe	118	16	can	can	MD
cord-326027-58whwspe	118	17	be	be	VB
cord-326027-58whwspe	118	18	detectable	detectable	JJ
cord-326027-58whwspe	118	19	by	by	IN
cord-326027-58whwspe	118	20	fluorescence	fluorescence	NN
cord-326027-58whwspe	118	21	.	.	.
cord-326027-58whwspe	119	1	To	to	TO
cord-326027-58whwspe	119	2	test	test	VB
cord-326027-58whwspe	119	3	if	if	IN
cord-326027-58whwspe	119	4	this	this	DT
cord-326027-58whwspe	119	5	was	be	VBD
cord-326027-58whwspe	119	6	the	the	DT
cord-326027-58whwspe	119	7	case	case	NN
cord-326027-58whwspe	119	8	,	,	,
cord-326027-58whwspe	119	9	virions	virion	NNS
cord-326027-58whwspe	119	10	purified	purify	VBN
cord-326027-58whwspe	119	11	by	by	IN
cord-326027-58whwspe	119	12	sedimentation	sedimentation	NN
cord-326027-58whwspe	119	13	through	through	IN
cord-326027-58whwspe	119	14	sucrose	sucrose	NN
cord-326027-58whwspe	119	15	cushion	cushion	NN
cord-326027-58whwspe	119	16	were	be	VBD
cord-326027-58whwspe	119	17	used	use	VBN
cord-326027-58whwspe	119	18	to	to	TO
cord-326027-58whwspe	119	19	infect	infect	VB
cord-326027-58whwspe	119	20	Vero	Vero	NNP
cord-326027-58whwspe	119	21	cells	cell	NNS
cord-326027-58whwspe	119	22	grown	grow	VBN
cord-326027-58whwspe	119	23	onto	onto	IN
cord-326027-58whwspe	119	24	a	a	DT
cord-326027-58whwspe	119	25	coverslip	coverslip	NN
cord-326027-58whwspe	119	26	at	at	IN
cord-326027-58whwspe	119	27	a	a	DT
cord-326027-58whwspe	119	28	multiplicity	multiplicity	NN
cord-326027-58whwspe	119	29	of	of	IN
cord-326027-58whwspe	119	30	10	10	CD
cord-326027-58whwspe	119	31	pfu	pfu	NN
cord-326027-58whwspe	119	32	/	/	SYM
cord-326027-58whwspe	119	33	cell	cell	NN
cord-326027-58whwspe	119	34	.	.	.
cord-326027-58whwspe	120	1	After	after	IN
cord-326027-58whwspe	120	2	synchronizing	synchronize	VBG
cord-326027-58whwspe	120	3	the	the	DT
cord-326027-58whwspe	120	4	infection	infection	NN
cord-326027-58whwspe	120	5	at	at	IN
cord-326027-58whwspe	120	6	4	4	CD
cord-326027-58whwspe	120	7	°	°	NN
cord-326027-58whwspe	120	8	C	c	NN
cord-326027-58whwspe	120	9	for	for	IN
cord-326027-58whwspe	120	10	2	2	CD
cord-326027-58whwspe	120	11	h	h	NN
cord-326027-58whwspe	120	12	,	,	,
cord-326027-58whwspe	120	13	cells	cell	NNS
cord-326027-58whwspe	120	14	were	be	VBD
cord-326027-58whwspe	120	15	incubated	incubate	VBN
cord-326027-58whwspe	120	16	for	for	IN
cord-326027-58whwspe	120	17	30	30	CD
cord-326027-58whwspe	120	18	min	min	NN
cord-326027-58whwspe	120	19	at	at	IN
cord-326027-58whwspe	120	20	37	37	CD
cord-326027-58whwspe	120	21	°	°	NN
cord-326027-58whwspe	120	22	C	c	NN
cord-326027-58whwspe	120	23	and	and	CC
cord-326027-58whwspe	120	24	subsequently	subsequently	RB
cord-326027-58whwspe	120	25	examined	examine	VBN
cord-326027-58whwspe	120	26	by	by	IN
cord-326027-58whwspe	120	27	fluorescence	fluorescence	NN
cord-326027-58whwspe	120	28	microscopy	microscopy	NN
cord-326027-58whwspe	120	29	.	.	.
cord-326027-58whwspe	121	1	Fluorescent	fluorescent	NN
cord-326027-58whwspe	121	2	particles	particle	NNS
cord-326027-58whwspe	121	3	,	,	,
cord-326027-58whwspe	121	4	visualized	visualize	VBN
cord-326027-58whwspe	121	5	as	as	IN
cord-326027-58whwspe	121	6	point	point	NN
cord-326027-58whwspe	121	7	sources	source	NNS
cord-326027-58whwspe	121	8	of	of	IN
cord-326027-58whwspe	121	9	GFP	GFP	NNP
cord-326027-58whwspe	121	10	fluorescence	fluorescence	NN
cord-326027-58whwspe	121	11	,	,	,
cord-326027-58whwspe	121	12	were	be	VBD
cord-326027-58whwspe	121	13	readily	readily	RB
cord-326027-58whwspe	121	14	detected	detect	VBN
cord-326027-58whwspe	121	15	inside	inside	IN
cord-326027-58whwspe	121	16	cell	cell	NN
cord-326027-58whwspe	121	17	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	121	18	(	(	-LRB-
cord-326027-58whwspe	121	19	Fig	Fig	NNP
cord-326027-58whwspe	121	20	.	.	.
cord-326027-58whwspe	121	21	2b	2b	NNP
cord-326027-58whwspe	121	22	)	)	-RRB-
cord-326027-58whwspe	121	23	.	.	.
cord-326027-58whwspe	122	1	When	when	WRB
cord-326027-58whwspe	122	2	target	target	NN
cord-326027-58whwspe	122	3	cells	cell	NNS
cord-326027-58whwspe	122	4	were	be	VBD
cord-326027-58whwspe	122	5	washed	wash	VBN
cord-326027-58whwspe	122	6	of	of	IN
cord-326027-58whwspe	122	7	free	free	JJ
cord-326027-58whwspe	122	8	virus	virus	NN
cord-326027-58whwspe	122	9	and	and	CC
cord-326027-58whwspe	122	10	allowed	allow	VBN
cord-326027-58whwspe	122	11	to	to	TO
cord-326027-58whwspe	122	12	incubate	incubate	VB
cord-326027-58whwspe	122	13	further	further	RB
cord-326027-58whwspe	122	14	at	at	IN
cord-326027-58whwspe	122	15	37	37	CD
cord-326027-58whwspe	122	16	°	°	NN
cord-326027-58whwspe	122	17	C	c	NN
cord-326027-58whwspe	122	18	,	,	,
cord-326027-58whwspe	122	19	a	a	DT
cord-326027-58whwspe	122	20	significant	significant	JJ
cord-326027-58whwspe	122	21	proportion	proportion	NN
cord-326027-58whwspe	122	22	of	of	IN
cord-326027-58whwspe	122	23	the	the	DT
cord-326027-58whwspe	122	24	GFP	GFP	NNP
cord-326027-58whwspe	122	25	signal	signal	NN
cord-326027-58whwspe	122	26	accumulated	accumulate	VBN
cord-326027-58whwspe	122	27	in	in	IN
cord-326027-58whwspe	122	28	the	the	DT
cord-326027-58whwspe	122	29	perinuclear	perinuclear	NNP
cord-326027-58whwspe	122	30	region	region	NN
cord-326027-58whwspe	122	31	,	,	,
cord-326027-58whwspe	122	32	in	in	IN
cord-326027-58whwspe	122	33	a	a	DT
cord-326027-58whwspe	122	34	short	short	JJ
cord-326027-58whwspe	122	35	time	time	NN
cord-326027-58whwspe	122	36	after	after	IN
cord-326027-58whwspe	122	37	infection	infection	NN
cord-326027-58whwspe	122	38	4	4	CD
cord-326027-58whwspe	122	39	h	h	NN
cord-326027-58whwspe	122	40	(	(	-LRB-
cord-326027-58whwspe	122	41	Additional	additional	JJ
cord-326027-58whwspe	122	42	file	file	NN
cord-326027-58whwspe	122	43	1	1	CD
cord-326027-58whwspe	122	44	)	)	-RRB-
cord-326027-58whwspe	122	45	.	.	.
cord-326027-58whwspe	123	1	Images	image	NNS
cord-326027-58whwspe	123	2	were	be	VBD
cord-326027-58whwspe	123	3	acquired	acquire	VBN
cord-326027-58whwspe	123	4	at	at	IN
cord-326027-58whwspe	123	5	30	30	CD
cord-326027-58whwspe	123	6	min	min	NN
cord-326027-58whwspe	123	7	and	and	CC
cord-326027-58whwspe	123	8	4	4	CD
cord-326027-58whwspe	123	9	h	h	NN
cord-326027-58whwspe	123	10	postinfection	postinfection	NN
cord-326027-58whwspe	123	11	,	,	,
cord-326027-58whwspe	123	12	before	before	IN
cord-326027-58whwspe	123	13	late	late	JJ
cord-326027-58whwspe	123	14	viral	viral	JJ
cord-326027-58whwspe	123	15	protein	protein	NN
cord-326027-58whwspe	123	16	expression	expression	NN
cord-326027-58whwspe	123	17	phase	phase	NN
cord-326027-58whwspe	123	18	onset	onset	NN
cord-326027-58whwspe	123	19	to	to	TO
cord-326027-58whwspe	123	20	avoid	avoid	VB
cord-326027-58whwspe	123	21	that	that	IN
cord-326027-58whwspe	123	22	observed	observe	VBD
cord-326027-58whwspe	123	23	GFP	GFP	NNP
cord-326027-58whwspe	123	24	signal	signal	NN
cord-326027-58whwspe	123	25	corresponds	correspond	VBZ
cord-326027-58whwspe	123	26	to	to	IN
cord-326027-58whwspe	123	27	newly	newly	RB
cord-326027-58whwspe	123	28	synthesized	synthesize	VBN
cord-326027-58whwspe	123	29	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	123	30	.	.	.
cord-326027-58whwspe	124	1	Some	some	DT
cord-326027-58whwspe	124	2	obvious	obvious	JJ
cord-326027-58whwspe	124	3	applications	application	NNS
cord-326027-58whwspe	124	4	of	of	IN
cord-326027-58whwspe	124	5	a	a	DT
cord-326027-58whwspe	124	6	virus	virus	NN
cord-326027-58whwspe	124	7	expressing	express	VBG
cord-326027-58whwspe	124	8	a	a	DT
cord-326027-58whwspe	124	9	fluorescent	fluorescent	JJ
cord-326027-58whwspe	124	10	structural	structural	JJ
cord-326027-58whwspe	124	11	protein	protein	NN
cord-326027-58whwspe	124	12	would	would	MD
cord-326027-58whwspe	124	13	be	be	VB
cord-326027-58whwspe	124	14	to	to	TO
cord-326027-58whwspe	124	15	localize	localize	VB
cord-326027-58whwspe	124	16	that	that	DT
cord-326027-58whwspe	124	17	protein	protein	NN
cord-326027-58whwspe	124	18	within	within	IN
cord-326027-58whwspe	124	19	the	the	DT
cord-326027-58whwspe	124	20	cell	cell	NN
cord-326027-58whwspe	124	21	and	and	CC
cord-326027-58whwspe	124	22	follow	follow	VB
cord-326027-58whwspe	124	23	its	-PRON-	PRP$
cord-326027-58whwspe	124	24	trafficking	trafficking	NN
cord-326027-58whwspe	124	25	during	during	IN
cord-326027-58whwspe	124	26	the	the	DT
cord-326027-58whwspe	124	27	infection	infection	NN
cord-326027-58whwspe	124	28	cycle	cycle	NN
cord-326027-58whwspe	124	29	and	and	CC
cord-326027-58whwspe	124	30	ultimately	ultimately	RB
cord-326027-58whwspe	124	31	,	,	,
cord-326027-58whwspe	124	32	to	to	TO
cord-326027-58whwspe	124	33	visualize	visualize	VB
cord-326027-58whwspe	124	34	the	the	DT
cord-326027-58whwspe	124	35	pathway	pathway	NN
cord-326027-58whwspe	124	36	of	of	IN
cord-326027-58whwspe	124	37	virus	virus	NNP
cord-326027-58whwspe	124	38	assembly	assembly	NN
cord-326027-58whwspe	124	39	in	in	IN
cord-326027-58whwspe	124	40	live	live	JJ
cord-326027-58whwspe	124	41	cells	cell	NNS
cord-326027-58whwspe	124	42	.	.	.
cord-326027-58whwspe	125	1	As	as	IN
cord-326027-58whwspe	125	2	previously	previously	RB
cord-326027-58whwspe	125	3	shown	show	VBN
cord-326027-58whwspe	125	4	,	,	,
cord-326027-58whwspe	125	5	wild	wild	JJ
cord-326027-58whwspe	125	6	-	-	HYPH
cord-326027-58whwspe	125	7	type	type	NN
cord-326027-58whwspe	125	8	p54	p54	NN
cord-326027-58whwspe	125	9	and	and	CC
cord-326027-58whwspe	125	10	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	125	11	distribution	distribution	NN
cord-326027-58whwspe	125	12	at	at	IN
cord-326027-58whwspe	125	13	late	late	JJ
cord-326027-58whwspe	125	14	times	time	NNS
cord-326027-58whwspe	125	15	in	in	IN
cord-326027-58whwspe	125	16	wild	wild	JJ
cord-326027-58whwspe	125	17	type	type	NN
cord-326027-58whwspe	125	18	and	and	CC
cord-326027-58whwspe	125	19	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	125	20	infection	infection	NN
cord-326027-58whwspe	125	21	was	be	VBD
cord-326027-58whwspe	125	22	shown	show	VBN
cord-326027-58whwspe	125	23	in	in	IN
cord-326027-58whwspe	125	24	perinuclear	perinuclear	JJ
cord-326027-58whwspe	125	25	viral	viral	JJ
cord-326027-58whwspe	125	26	factories	factory	NNS
cord-326027-58whwspe	125	27	(	(	-LRB-
cord-326027-58whwspe	125	28	Figs	fig	NNS
cord-326027-58whwspe	125	29	.	.	.
cord-326027-58whwspe	126	1	1e	1e	NNP
cord-326027-58whwspe	126	2	-	-	HYPH
cord-326027-58whwspe	126	3	j	j	NNP
cord-326027-58whwspe	126	4	)	)	-RRB-
cord-326027-58whwspe	126	5	.	.	.
cord-326027-58whwspe	127	1	We	-PRON-	PRP
cord-326027-58whwspe	127	2	examined	examine	VBD
cord-326027-58whwspe	127	3	this	this	DT
cord-326027-58whwspe	127	4	distribution	distribution	NN
cord-326027-58whwspe	127	5	for	for	IN
cord-326027-58whwspe	127	6	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	127	7	infected	infected	JJ
cord-326027-58whwspe	127	8	cells	cell	NNS
cord-326027-58whwspe	127	9	at	at	IN
cord-326027-58whwspe	127	10	12	12	CD
cord-326027-58whwspe	127	11	-	-	SYM
cord-326027-58whwspe	127	12	16	16	CD
cord-326027-58whwspe	127	13	hpi	hpi	NN
cord-326027-58whwspe	127	14	by	by	IN
cord-326027-58whwspe	127	15	confocal	confocal	JJ
cord-326027-58whwspe	127	16	fluorescence	fluorescence	NN
cord-326027-58whwspe	127	17	microscopy	microscopy	NNS
cord-326027-58whwspe	127	18	in	in	IN
cord-326027-58whwspe	127	19	fixed	fix	VBN
cord-326027-58whwspe	127	20	cells	cell	NNS
cord-326027-58whwspe	127	21	.	.	.
cord-326027-58whwspe	128	1	Protein	protein	NN
cord-326027-58whwspe	128	2	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	128	3	distribution	distribution	NN
cord-326027-58whwspe	128	4	coincided	coincide	VBN
cord-326027-58whwspe	128	5	with	with	IN
cord-326027-58whwspe	128	6	assembly	assembly	NN
cord-326027-58whwspe	128	7	sites	site	NNS
cord-326027-58whwspe	128	8	in	in	IN
cord-326027-58whwspe	128	9	the	the	DT
cord-326027-58whwspe	128	10	viral	viral	JJ
cord-326027-58whwspe	128	11	factories	factory	NNS
cord-326027-58whwspe	128	12	,	,	,
cord-326027-58whwspe	128	13	which	which	WDT
cord-326027-58whwspe	128	14	is	be	VBZ
cord-326027-58whwspe	128	15	characterized	characterize	VBN
cord-326027-58whwspe	128	16	by	by	IN
cord-326027-58whwspe	128	17	a	a	DT
cord-326027-58whwspe	128	18	great	great	JJ
cord-326027-58whwspe	128	19	accumulation	accumulation	NN
cord-326027-58whwspe	128	20	of	of	IN
cord-326027-58whwspe	128	21	newly	newly	RB
cord-326027-58whwspe	128	22	synthesized	synthesize	VBN
cord-326027-58whwspe	128	23	viral	viral	JJ
cord-326027-58whwspe	128	24	DNA	dna	NN
cord-326027-58whwspe	128	25	.	.	.
cord-326027-58whwspe	129	1	To	to	TO
cord-326027-58whwspe	129	2	further	further	RB
cord-326027-58whwspe	129	3	confirm	confirm	VB
cord-326027-58whwspe	129	4	that	that	IN
cord-326027-58whwspe	129	5	p54-EGFP	p54-egfp	JJ
cord-326027-58whwspe	129	6	distribution	distribution	NN
cord-326027-58whwspe	129	7	could	could	MD
cord-326027-58whwspe	129	8	be	be	VB
cord-326027-58whwspe	129	9	used	use	VBN
cord-326027-58whwspe	129	10	as	as	IN
cord-326027-58whwspe	129	11	a	a	DT
cord-326027-58whwspe	129	12	novel	novel	JJ
cord-326027-58whwspe	129	13	viral	viral	JJ
cord-326027-58whwspe	129	14	factory	factory	NN
cord-326027-58whwspe	129	15	marker	marker	NN
cord-326027-58whwspe	129	16	in	in	IN
cord-326027-58whwspe	129	17	ASFV	ASFV	NNP
cord-326027-58whwspe	129	18	infection	infection	NN
cord-326027-58whwspe	129	19	we	-PRON-	PRP
cord-326027-58whwspe	129	20	showed	show	VBD
cord-326027-58whwspe	129	21	that	that	IN
cord-326027-58whwspe	129	22	the	the	DT
cord-326027-58whwspe	129	23	Golgi	Golgi	NNP
cord-326027-58whwspe	129	24	complex	complex	NN
cord-326027-58whwspe	129	25	in	in	IN
cord-326027-58whwspe	129	26	living	live	VBG
cord-326027-58whwspe	129	27	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	129	28	infected	infect	VBN
cord-326027-58whwspe	129	29	cells	cell	NNS
cord-326027-58whwspe	129	30	was	be	VBD
cord-326027-58whwspe	129	31	located	locate	VBN
cord-326027-58whwspe	129	32	surrounding	surround	VBG
cord-326027-58whwspe	129	33	,	,	,
cord-326027-58whwspe	129	34	but	but	CC
cord-326027-58whwspe	129	35	not	not	RB
cord-326027-58whwspe	129	36	coincident	coincident	JJ
cord-326027-58whwspe	129	37	with	with	IN
cord-326027-58whwspe	129	38	the	the	DT
cord-326027-58whwspe	129	39	viral	viral	JJ
cord-326027-58whwspe	129	40	factory	factory	NN
cord-326027-58whwspe	129	41	where	where	WRB
cord-326027-58whwspe	129	42	a	a	DT
cord-326027-58whwspe	129	43	great	great	JJ
cord-326027-58whwspe	129	44	accumulation	accumulation	NN
cord-326027-58whwspe	129	45	of	of	IN
cord-326027-58whwspe	129	46	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	129	47	is	be	VBZ
cord-326027-58whwspe	129	48	found	find	VBN
cord-326027-58whwspe	129	49	(	(	-LRB-
cord-326027-58whwspe	129	50	Figs	fig	NNS
cord-326027-58whwspe	129	51	.	.	.
cord-326027-58whwspe	130	1	4e	4e	LS
cord-326027-58whwspe	130	2	-	-	HYPH
cord-326027-58whwspe	130	3	g	g	NNP
cord-326027-58whwspe	130	4	)	)	-RRB-
cord-326027-58whwspe	130	5	.	.	.
cord-326027-58whwspe	131	1	The	the	DT
cord-326027-58whwspe	131	2	ultimate	ultimate	JJ
cord-326027-58whwspe	131	3	elegance	elegance	NN
cord-326027-58whwspe	131	4	of	of	IN
cord-326027-58whwspe	131	5	a	a	DT
cord-326027-58whwspe	131	6	GFP	GFP	NNP
cord-326027-58whwspe	131	7	-	-	HYPH
cord-326027-58whwspe	131	8	incorporating	incorporate	VBG
cord-326027-58whwspe	131	9	virus	virus	NN
cord-326027-58whwspe	131	10	is	be	VBZ
cord-326027-58whwspe	131	11	the	the	DT
cord-326027-58whwspe	131	12	potential	potential	NN
cord-326027-58whwspe	131	13	for	for	IN
cord-326027-58whwspe	131	14	monitoring	monitor	VBG
cord-326027-58whwspe	131	15	virus	virus	NN
cord-326027-58whwspe	131	16	infection	infection	NN
cord-326027-58whwspe	131	17	in	in	IN
cord-326027-58whwspe	131	18	individual	individual	JJ
cord-326027-58whwspe	131	19	living	living	NN
cord-326027-58whwspe	131	20	cells	cell	NNS
cord-326027-58whwspe	131	21	.	.	.
cord-326027-58whwspe	132	1	The	the	DT
cord-326027-58whwspe	132	2	abundant	abundant	JJ
cord-326027-58whwspe	132	3	incorporation	incorporation	NN
cord-326027-58whwspe	132	4	of	of	IN
cord-326027-58whwspe	132	5	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	132	6	into	into	IN
cord-326027-58whwspe	132	7	ASFV	ASFV	NNP
cord-326027-58whwspe	132	8	particles	particle	NNS
cord-326027-58whwspe	132	9	indicated	indicate	VBD
cord-326027-58whwspe	132	10	that	that	IN
cord-326027-58whwspe	132	11	it	-PRON-	PRP
cord-326027-58whwspe	132	12	should	should	MD
cord-326027-58whwspe	132	13	be	be	VB
cord-326027-58whwspe	132	14	possible	possible	JJ
cord-326027-58whwspe	132	15	to	to	TO
cord-326027-58whwspe	132	16	visualize	visualize	VB
cord-326027-58whwspe	132	17	the	the	DT
cord-326027-58whwspe	132	18	intracellular	intracellular	JJ
cord-326027-58whwspe	132	19	movement	movement	NN
cord-326027-58whwspe	132	20	of	of	IN
cord-326027-58whwspe	132	21	virions	virion	NNS
cord-326027-58whwspe	132	22	in	in	IN
cord-326027-58whwspe	132	23	real	real	JJ
cord-326027-58whwspe	132	24	time	time	NN
cord-326027-58whwspe	132	25	by	by	IN
cord-326027-58whwspe	132	26	laser	laser	NN
cord-326027-58whwspe	132	27	-	-	HYPH
cord-326027-58whwspe	132	28	scanning	scan	VBG
cord-326027-58whwspe	132	29	confocal	confocal	JJ
cord-326027-58whwspe	132	30	microscopy	microscopy	NNS
cord-326027-58whwspe	132	31	of	of	IN
cord-326027-58whwspe	132	32	live	live	JJ
cord-326027-58whwspe	132	33	cells	cell	NNS
cord-326027-58whwspe	132	34	.	.	.
cord-326027-58whwspe	133	1	Approximately	approximately	RB
cord-326027-58whwspe	133	2	50	50	CD
cord-326027-58whwspe	133	3	%	%	NN
cord-326027-58whwspe	133	4	confluent	confluent	JJ
cord-326027-58whwspe	133	5	Vero	Vero	NNP
cord-326027-58whwspe	133	6	cells	cell	NNS
cord-326027-58whwspe	133	7	were	be	VBD
cord-326027-58whwspe	133	8	infected	infect	VBN
cord-326027-58whwspe	133	9	with	with	IN
cord-326027-58whwspe	133	10	more	more	JJR
cord-326027-58whwspe	133	11	than	than	IN
cord-326027-58whwspe	133	12	10	10	CD
cord-326027-58whwspe	133	13	PFU	pfu	NN
cord-326027-58whwspe	133	14	of	of	IN
cord-326027-58whwspe	133	15	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	133	16	virus	virus	NN
cord-326027-58whwspe	133	17	per	per	IN
cord-326027-58whwspe	133	18	cell	cell	NN
cord-326027-58whwspe	133	19	.	.	.
cord-326027-58whwspe	134	1	Virus	virus	NN
cord-326027-58whwspe	134	2	infection	infection	NN
cord-326027-58whwspe	134	3	was	be	VBD
cord-326027-58whwspe	134	4	synchronized	synchronize	VBN
cord-326027-58whwspe	134	5	by	by	IN
cord-326027-58whwspe	134	6	incubation	incubation	NN
cord-326027-58whwspe	134	7	of	of	IN
cord-326027-58whwspe	134	8	cells	cell	NNS
cord-326027-58whwspe	134	9	at	at	IN
cord-326027-58whwspe	134	10	4	4	CD
cord-326027-58whwspe	134	11	°	°	NN
cord-326027-58whwspe	134	12	C	c	NN
cord-326027-58whwspe	134	13	for	for	IN
cord-326027-58whwspe	134	14	90	90	CD
cord-326027-58whwspe	134	15	min	min	NNS
cord-326027-58whwspe	134	16	and	and	CC
cord-326027-58whwspe	134	17	images	image	NNS
cord-326027-58whwspe	134	18	were	be	VBD
cord-326027-58whwspe	134	19	collected	collect	VBN
cord-326027-58whwspe	134	20	,	,	,
cord-326027-58whwspe	134	21	after	after	IN
cord-326027-58whwspe	134	22	incubation	incubation	NN
cord-326027-58whwspe	134	23	at	at	IN
cord-326027-58whwspe	134	24	37	37	CD
cord-326027-58whwspe	134	25	°	°	NN
cord-326027-58whwspe	134	26	C	c	NN
cord-326027-58whwspe	134	27	for	for	IN
cord-326027-58whwspe	134	28	nearly	nearly	RB
cord-326027-58whwspe	134	29	2	2	CD
cord-326027-58whwspe	134	30	h	h	NN
cord-326027-58whwspe	134	31	,	,	,
cord-326027-58whwspe	134	32	to	to	TO
cord-326027-58whwspe	134	33	analyze	analyze	VB
cord-326027-58whwspe	134	34	the	the	DT
cord-326027-58whwspe	134	35	first	first	JJ
cord-326027-58whwspe	134	36	steps	step	NNS
cord-326027-58whwspe	134	37	of	of	IN
cord-326027-58whwspe	134	38	infection	infection	NN
cord-326027-58whwspe	134	39	at	at	IN
cord-326027-58whwspe	134	40	high	high	JJ
cord-326027-58whwspe	134	41	magnification	magnification	NN
cord-326027-58whwspe	134	42	.	.	.
cord-326027-58whwspe	135	1	Individual	individual	JJ
cord-326027-58whwspe	135	2	virus	virus	NN
cord-326027-58whwspe	135	3	particles	particle	NNS
cord-326027-58whwspe	135	4	were	be	VBD
cord-326027-58whwspe	135	5	readily	readily	RB
cord-326027-58whwspe	135	6	observed	observe	VBN
cord-326027-58whwspe	135	7	and	and	CC
cord-326027-58whwspe	135	8	images	image	NNS
cord-326027-58whwspe	135	9	were	be	VBD
cord-326027-58whwspe	135	10	acquired	acquire	VBN
cord-326027-58whwspe	135	11	with	with	IN
cord-326027-58whwspe	135	12	the	the	DT
cord-326027-58whwspe	135	13	confocal	confocal	JJ
cord-326027-58whwspe	135	14	microscope	microscope	NN
cord-326027-58whwspe	135	15	setup	setup	NN
cord-326027-58whwspe	135	16	at	at	IN
cord-326027-58whwspe	135	17	a	a	DT
cord-326027-58whwspe	135	18	rate	rate	NN
cord-326027-58whwspe	135	19	of	of	IN
cord-326027-58whwspe	135	20	1	1	CD
cord-326027-58whwspe	135	21	frame/3s	frame/3s	NN
cord-326027-58whwspe	135	22	for	for	IN
cord-326027-58whwspe	135	23	a	a	DT
cord-326027-58whwspe	135	24	maximum	maximum	NN
cord-326027-58whwspe	135	25	of	of	IN
cord-326027-58whwspe	135	26	2	2	CD
cord-326027-58whwspe	135	27	min	min	NN
cord-326027-58whwspe	135	28	.	.	.
cord-326027-58whwspe	136	1	With	with	IN
cord-326027-58whwspe	136	2	this	this	DT
cord-326027-58whwspe	136	3	short	short	JJ
cord-326027-58whwspe	136	4	interval	interval	NN
cord-326027-58whwspe	136	5	of	of	IN
cord-326027-58whwspe	136	6	time	time	NN
cord-326027-58whwspe	136	7	,	,	,
cord-326027-58whwspe	136	8	we	-PRON-	PRP
cord-326027-58whwspe	136	9	discerned	discern	VBD
cord-326027-58whwspe	136	10	that	that	IN
cord-326027-58whwspe	136	11	intracellular	intracellular	JJ
cord-326027-58whwspe	136	12	movement	movement	NN
cord-326027-58whwspe	136	13	in	in	IN
cord-326027-58whwspe	136	14	the	the	DT
cord-326027-58whwspe	136	15	proximity	proximity	NN
cord-326027-58whwspe	136	16	of	of	IN
cord-326027-58whwspe	136	17	perinuclear	perinuclear	NN
cord-326027-58whwspe	136	18	areas	area	NNS
cord-326027-58whwspe	136	19	was	be	VBD
cord-326027-58whwspe	136	20	composed	compose	VBN
cord-326027-58whwspe	136	21	of	of	IN
cord-326027-58whwspe	136	22	frequent	frequent	JJ
cord-326027-58whwspe	136	23	stops	stop	NNS
cord-326027-58whwspe	136	24	and	and	CC
cord-326027-58whwspe	136	25	starts	start	VBZ
cord-326027-58whwspe	136	26	,	,	,
cord-326027-58whwspe	136	27	and	and	CC
cord-326027-58whwspe	136	28	often	often	RB
cord-326027-58whwspe	136	29	without	without	IN
cord-326027-58whwspe	136	30	any	any	DT
cord-326027-58whwspe	136	31	apparent	apparent	JJ
cord-326027-58whwspe	136	32	direction	direction	NN
cord-326027-58whwspe	136	33	.	.	.
cord-326027-58whwspe	137	1	Fig	Fig	NNP
cord-326027-58whwspe	137	2	.	.	.
cord-326027-58whwspe	138	1	5	5	CD
cord-326027-58whwspe	138	2	shows	show	VBZ
cord-326027-58whwspe	138	3	an	an	DT
cord-326027-58whwspe	138	4	example	example	NN
cord-326027-58whwspe	138	5	of	of	IN
cord-326027-58whwspe	138	6	this	this	DT
cord-326027-58whwspe	138	7	intermittent	intermittent	JJ
cord-326027-58whwspe	138	8	or	or	CC
cord-326027-58whwspe	138	9	saltatory	saltatory	JJ
cord-326027-58whwspe	138	10	movement	movement	NN
cord-326027-58whwspe	138	11	of	of	IN
cord-326027-58whwspe	138	12	an	an	DT
cord-326027-58whwspe	138	13	individual	individual	JJ
cord-326027-58whwspe	138	14	virion	virion	NN
cord-326027-58whwspe	138	15	.	.	.
cord-326027-58whwspe	139	1	Over	over	IN
cord-326027-58whwspe	139	2	the	the	DT
cord-326027-58whwspe	139	3	times	time	NNS
cord-326027-58whwspe	139	4	and	and	CC
cord-326027-58whwspe	139	5	distances	distance	NNS
cord-326027-58whwspe	139	6	measured	measure	VBN
cord-326027-58whwspe	139	7	in	in	IN
cord-326027-58whwspe	139	8	following	follow	VBG
cord-326027-58whwspe	139	9	frames	frame	NNS
cord-326027-58whwspe	139	10	acquired	acquire	VBN
cord-326027-58whwspe	139	11	from	from	IN
cord-326027-58whwspe	139	12	10	10	CD
cord-326027-58whwspe	139	13	different	different	JJ
cord-326027-58whwspe	139	14	virions	virion	NNS
cord-326027-58whwspe	139	15	,	,	,
cord-326027-58whwspe	139	16	an	an	DT
cord-326027-58whwspe	139	17	average	average	JJ
cord-326027-58whwspe	139	18	speed	speed	NN
cord-326027-58whwspe	139	19	ranged	range	VBD
cord-326027-58whwspe	139	20	from	from	IN
cord-326027-58whwspe	139	21	0.2	0.2	CD
cord-326027-58whwspe	139	22	to	to	IN
cord-326027-58whwspe	139	23	0.5	0.5	CD
cord-326027-58whwspe	139	24	μm	μm	NN
cord-326027-58whwspe	139	25	/	/	SYM
cord-326027-58whwspe	139	26	s	s	NN
cord-326027-58whwspe	139	27	at	at	IN
cord-326027-58whwspe	139	28	this	this	DT
cord-326027-58whwspe	139	29	stage	stage	NN
cord-326027-58whwspe	139	30	of	of	IN
cord-326027-58whwspe	139	31	infection	infection	NN
cord-326027-58whwspe	139	32	was	be	VBD
cord-326027-58whwspe	139	33	calculated	calculate	VBN
cord-326027-58whwspe	139	34	.	.	.
cord-326027-58whwspe	140	1	In	in	IN
cord-326027-58whwspe	140	2	order	order	NN
cord-326027-58whwspe	140	3	to	to	TO
cord-326027-58whwspe	140	4	test	test	VB
cord-326027-58whwspe	140	5	the	the	DT
cord-326027-58whwspe	140	6	role	role	NN
cord-326027-58whwspe	140	7	of	of	IN
cord-326027-58whwspe	140	8	microtubules	microtubule	NNS
cord-326027-58whwspe	140	9	at	at	IN
cord-326027-58whwspe	140	10	this	this	DT
cord-326027-58whwspe	140	11	stage	stage	NN
cord-326027-58whwspe	140	12	of	of	IN
cord-326027-58whwspe	140	13	infection	infection	NN
cord-326027-58whwspe	140	14	,	,	,
cord-326027-58whwspe	140	15	disruption	disruption	NN
cord-326027-58whwspe	140	16	of	of	IN
cord-326027-58whwspe	140	17	microtubules	microtubule	NNS
cord-326027-58whwspe	140	18	with	with	IN
cord-326027-58whwspe	140	19	10	10	CD
cord-326027-58whwspe	140	20	μM	μm	CD
cord-326027-58whwspe	140	21	nocodazole	nocodazole	NNP
cord-326027-58whwspe	140	22	was	be	VBD
cord-326027-58whwspe	140	23	achieved	achieve	VBN
cord-326027-58whwspe	140	24	prior	prior	RB
cord-326027-58whwspe	140	25	to	to	IN
cord-326027-58whwspe	140	26	infection	infection	NN
cord-326027-58whwspe	140	27	with	with	IN
cord-326027-58whwspe	140	28	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	140	29	.	.	.
cord-326027-58whwspe	141	1	Under	under	IN
cord-326027-58whwspe	141	2	these	these	DT
cord-326027-58whwspe	141	3	conditions	condition	NNS
cord-326027-58whwspe	141	4	we	-PRON-	PRP
cord-326027-58whwspe	141	5	could	could	MD
cord-326027-58whwspe	141	6	not	not	RB
cord-326027-58whwspe	141	7	track	track	VB
cord-326027-58whwspe	141	8	any	any	DT
cord-326027-58whwspe	141	9	virion	virion	NN
cord-326027-58whwspe	141	10	movement	movement	NN
cord-326027-58whwspe	141	11	examined	examine	VBN
cord-326027-58whwspe	141	12	at	at	IN
cord-326027-58whwspe	141	13	1	1	CD
cord-326027-58whwspe	141	14	hpi	hpi	NNP
cord-326027-58whwspe	141	15	or	or	CC
cord-326027-58whwspe	141	16	4	4	CD
cord-326027-58whwspe	141	17	hpi	hpi	NNS
cord-326027-58whwspe	141	18	in	in	IN
cord-326027-58whwspe	141	19	live	live	JJ
cord-326027-58whwspe	141	20	cells	cell	NNS
cord-326027-58whwspe	141	21	.	.	.
cord-326027-58whwspe	142	1	Interestingly	interestingly	RB
cord-326027-58whwspe	142	2	,	,	,
cord-326027-58whwspe	142	3	at	at	IN
cord-326027-58whwspe	142	4	mentioned	mention	VBN
cord-326027-58whwspe	142	5	times	time	NNS
cord-326027-58whwspe	142	6	postinfection	postinfection	NN
cord-326027-58whwspe	142	7	most	most	JJS
cord-326027-58whwspe	142	8	GFP	GFP	NNP
cord-326027-58whwspe	142	9	signal	signal	NN
cord-326027-58whwspe	142	10	was	be	VBD
cord-326027-58whwspe	142	11	localized	localize	VBN
cord-326027-58whwspe	142	12	far	far	RB
cord-326027-58whwspe	142	13	from	from	IN
cord-326027-58whwspe	142	14	perinuclear	perinuclear	JJ
cord-326027-58whwspe	142	15	areas	area	NNS
cord-326027-58whwspe	142	16	in	in	IN
cord-326027-58whwspe	142	17	contrast	contrast	NN
cord-326027-58whwspe	142	18	to	to	IN
cord-326027-58whwspe	142	19	infected	infected	JJ
cord-326027-58whwspe	142	20	cells	cell	NNS
cord-326027-58whwspe	142	21	with	with	IN
cord-326027-58whwspe	142	22	intact	intact	JJ
cord-326027-58whwspe	142	23	microtubules	microtubule	NNS
cord-326027-58whwspe	142	24	(	(	-LRB-
cord-326027-58whwspe	142	25	Additional	additional	JJ
cord-326027-58whwspe	142	26	file	file	NN
cord-326027-58whwspe	142	27	2	2	CD
cord-326027-58whwspe	142	28	)	)	-RRB-
cord-326027-58whwspe	142	29	.	.	.
cord-326027-58whwspe	143	1	To	to	TO
cord-326027-58whwspe	143	2	determine	determine	VB
cord-326027-58whwspe	143	3	the	the	DT
cord-326027-58whwspe	143	4	stage	stage	NN
cord-326027-58whwspe	143	5	of	of	IN
cord-326027-58whwspe	143	6	infection	infection	NN
cord-326027-58whwspe	143	7	at	at	IN
cord-326027-58whwspe	143	8	which	which	WDT
cord-326027-58whwspe	143	9	newly	newly	RB
cord-326027-58whwspe	143	10	synthesized	synthesize	VBN
cord-326027-58whwspe	143	11	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	143	12	could	could	MD
cord-326027-58whwspe	143	13	initially	initially	RB
cord-326027-58whwspe	143	14	be	be	VB
cord-326027-58whwspe	143	15	detected	detect	VBN
cord-326027-58whwspe	143	16	by	by	IN
cord-326027-58whwspe	143	17	direct	direct	JJ
cord-326027-58whwspe	143	18	fluorescence	fluorescence	NN
cord-326027-58whwspe	143	19	microscopy	microscopy	NN
cord-326027-58whwspe	143	20	,	,	,
cord-326027-58whwspe	143	21	and	and	CC
cord-326027-58whwspe	143	22	to	to	TO
cord-326027-58whwspe	143	23	visualize	visualize	VB
cord-326027-58whwspe	143	24	virus	virus	NN
cord-326027-58whwspe	143	25	factory	factory	NN
cord-326027-58whwspe	143	26	constitution	constitution	NN
cord-326027-58whwspe	143	27	,	,	,
cord-326027-58whwspe	143	28	Vero	Vero	NNP
cord-326027-58whwspe	143	29	cells	cell	NNS
cord-326027-58whwspe	143	30	were	be	VBD
cord-326027-58whwspe	143	31	infected	infect	VBN
cord-326027-58whwspe	143	32	with	with	IN
cord-326027-58whwspe	143	33	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	143	34	at	at	IN
cord-326027-58whwspe	143	35	a	a	DT
cord-326027-58whwspe	143	36	multiplicity	multiplicity	NN
cord-326027-58whwspe	143	37	of	of	IN
cord-326027-58whwspe	143	38	infection	infection	NN
cord-326027-58whwspe	143	39	of	of	IN
cord-326027-58whwspe	143	40	1	1	CD
cord-326027-58whwspe	143	41	pfu	pfu	NN
cord-326027-58whwspe	143	42	/	/	SYM
cord-326027-58whwspe	143	43	cell	cell	NN
cord-326027-58whwspe	143	44	.	.	.
cord-326027-58whwspe	144	1	After	after	IN
cord-326027-58whwspe	144	2	synchronization	synchronization	NN
cord-326027-58whwspe	144	3	of	of	IN
cord-326027-58whwspe	144	4	the	the	DT
cord-326027-58whwspe	144	5	infection	infection	NN
cord-326027-58whwspe	144	6	,	,	,
cord-326027-58whwspe	144	7	cells	cell	NNS
cord-326027-58whwspe	144	8	were	be	VBD
cord-326027-58whwspe	144	9	examined	examine	VBN
cord-326027-58whwspe	144	10	for	for	IN
cord-326027-58whwspe	144	11	EGFP	EGFP	NNP
cord-326027-58whwspe	144	12	fluorescence	fluorescence	NN
cord-326027-58whwspe	144	13	every	every	DT
cord-326027-58whwspe	144	14	Fig	Fig	NNP
cord-326027-58whwspe	144	15	.	.	NNP
cord-326027-58whwspe	144	16	6	6	CD
cord-326027-58whwspe	144	17	.	.	.
cord-326027-58whwspe	145	1	Protein	protein	NN
cord-326027-58whwspe	145	2	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	145	3	was	be	VBD
cord-326027-58whwspe	145	4	first	first	RB
cord-326027-58whwspe	145	5	observed	observe	VBN
cord-326027-58whwspe	145	6	in	in	IN
cord-326027-58whwspe	145	7	the	the	DT
cord-326027-58whwspe	145	8	majority	majority	NN
cord-326027-58whwspe	145	9	of	of	IN
cord-326027-58whwspe	145	10	cells	cell	NNS
cord-326027-58whwspe	145	11	as	as	RB
cord-326027-58whwspe	145	12	early	early	RB
cord-326027-58whwspe	145	13	as	as	IN
cord-326027-58whwspe	145	14	7	7	CD
cord-326027-58whwspe	145	15	-	-	SYM
cord-326027-58whwspe	145	16	8	8	CD
cord-326027-58whwspe	145	17	hpi	hpi	NNS
cord-326027-58whwspe	145	18	.	.	.
cord-326027-58whwspe	146	1	The	the	DT
cord-326027-58whwspe	146	2	protein	protein	NN
cord-326027-58whwspe	146	3	was	be	VBD
cord-326027-58whwspe	146	4	mainly	mainly	RB
cord-326027-58whwspe	146	5	localized	localize	VBN
cord-326027-58whwspe	146	6	as	as	IN
cord-326027-58whwspe	146	7	small	small	JJ
cord-326027-58whwspe	146	8	,	,	,
cord-326027-58whwspe	146	9	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	146	10	and	and	CC
cord-326027-58whwspe	146	11	fluorescent	fluorescent	JJ
cord-326027-58whwspe	146	12	aggregates	aggregate	NNS
cord-326027-58whwspe	146	13	around	around	IN
cord-326027-58whwspe	146	14	the	the	DT
cord-326027-58whwspe	146	15	nucleus	nucleus	NN
cord-326027-58whwspe	146	16	(	(	-LRB-
cord-326027-58whwspe	146	17	2	2	CD
cord-326027-58whwspe	146	18	or	or	CC
cord-326027-58whwspe	146	19	3	3	CD
cord-326027-58whwspe	146	20	spots	spot	NNS
cord-326027-58whwspe	146	21	were	be	VBD
cord-326027-58whwspe	146	22	usually	usually	RB
cord-326027-58whwspe	146	23	observed	observe	VBN
cord-326027-58whwspe	146	24	within	within	IN
cord-326027-58whwspe	146	25	single	single	JJ
cord-326027-58whwspe	146	26	cell	cell	NN
cord-326027-58whwspe	146	27	)	)	-RRB-
cord-326027-58whwspe	146	28	.	.	.
cord-326027-58whwspe	147	1	From	from	IN
cord-326027-58whwspe	147	2	this	this	DT
cord-326027-58whwspe	147	3	point	point	NN
cord-326027-58whwspe	147	4	,	,	,
cord-326027-58whwspe	147	5	all	all	DT
cord-326027-58whwspe	147	6	of	of	IN
cord-326027-58whwspe	147	7	these	these	DT
cord-326027-58whwspe	147	8	perinuclear	perinuclear	JJ
cord-326027-58whwspe	147	9	spots	spot	NNS
cord-326027-58whwspe	147	10	finally	finally	RB
cord-326027-58whwspe	147	11	aggregated	aggregate	VBN
cord-326027-58whwspe	147	12	along	along	IN
cord-326027-58whwspe	147	13	the	the	DT
cord-326027-58whwspe	147	14	next	next	JJ
cord-326027-58whwspe	147	15	2	2	CD
cord-326027-58whwspe	147	16	h	h	NN
cord-326027-58whwspe	147	17	to	to	TO
cord-326027-58whwspe	147	18	give	give	VB
cord-326027-58whwspe	147	19	rise	rise	NN
cord-326027-58whwspe	147	20	to	to	IN
cord-326027-58whwspe	147	21	a	a	DT
cord-326027-58whwspe	147	22	unique	unique	JJ
cord-326027-58whwspe	147	23	brilliant	brilliant	JJ
cord-326027-58whwspe	147	24	spot	spot	NN
cord-326027-58whwspe	147	25	close	close	RB
cord-326027-58whwspe	147	26	to	to	IN
cord-326027-58whwspe	147	27	the	the	DT
cord-326027-58whwspe	147	28	nucleus	nucleus	NN
cord-326027-58whwspe	148	1	(	(	-LRB-
cord-326027-58whwspe	148	2	Fig	Fig	NNP
cord-326027-58whwspe	148	3	.	.	.
cord-326027-58whwspe	148	4	6b	6b	CD
cord-326027-58whwspe	148	5	)	)	-RRB-
cord-326027-58whwspe	148	6	.	.	.
cord-326027-58whwspe	149	1	This	this	DT
cord-326027-58whwspe	149	2	accumulation	accumulation	NN
cord-326027-58whwspe	149	3	of	of	IN
cord-326027-58whwspe	149	4	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	149	5	,	,	,
cord-326027-58whwspe	149	6	which	which	WDT
cord-326027-58whwspe	149	7	was	be	VBD
cord-326027-58whwspe	149	8	previously	previously	RB
cord-326027-58whwspe	149	9	shown	show	VBN
cord-326027-58whwspe	149	10	coincident	coincident	JJ
cord-326027-58whwspe	149	11	to	to	IN
cord-326027-58whwspe	149	12	the	the	DT
cord-326027-58whwspe	149	13	viral	viral	JJ
cord-326027-58whwspe	149	14	factory	factory	NN
cord-326027-58whwspe	149	15	,	,	,
cord-326027-58whwspe	149	16	gradually	gradually	RB
cord-326027-58whwspe	149	17	increased	increase	VBD
cord-326027-58whwspe	149	18	in	in	IN
cord-326027-58whwspe	149	19	size	size	NN
cord-326027-58whwspe	149	20	and	and	CC
cord-326027-58whwspe	149	21	fluorescence	fluorescence	NN
cord-326027-58whwspe	149	22	intensity	intensity	NN
cord-326027-58whwspe	149	23	as	as	IN
cord-326027-58whwspe	149	24	infection	infection	NN
cord-326027-58whwspe	149	25	proceeded	proceed	VBD
cord-326027-58whwspe	149	26	.	.	.
cord-326027-58whwspe	150	1	It	-PRON-	PRP
cord-326027-58whwspe	150	2	should	should	MD
cord-326027-58whwspe	150	3	be	be	VB
cord-326027-58whwspe	150	4	mentioned	mention	VBN
cord-326027-58whwspe	150	5	that	that	DT
cord-326027-58whwspe	150	6	aggregation	aggregation	NN
cord-326027-58whwspe	150	7	of	of	IN
cord-326027-58whwspe	150	8	initial	initial	JJ
cord-326027-58whwspe	150	9	perinuclear	perinuclear	JJ
cord-326027-58whwspe	150	10	spots	spot	NNS
cord-326027-58whwspe	150	11	of	of	IN
cord-326027-58whwspe	150	12	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	150	13	could	could	MD
cord-326027-58whwspe	150	14	not	not	RB
cord-326027-58whwspe	150	15	be	be	VB
cord-326027-58whwspe	150	16	observed	observe	VBN
cord-326027-58whwspe	150	17	in	in	IN
cord-326027-58whwspe	150	18	every	every	DT
cord-326027-58whwspe	150	19	infected	infected	JJ
cord-326027-58whwspe	150	20	cell	cell	NN
cord-326027-58whwspe	150	21	,	,	,
cord-326027-58whwspe	150	22	but	but	CC
cord-326027-58whwspe	150	23	in	in	IN
cord-326027-58whwspe	150	24	most	most	JJS
cord-326027-58whwspe	150	25	of	of	IN
cord-326027-58whwspe	150	26	them	-PRON-	PRP
cord-326027-58whwspe	150	27	,	,	,
cord-326027-58whwspe	150	28	probably	probably	RB
cord-326027-58whwspe	150	29	due	due	IN
cord-326027-58whwspe	150	30	to	to	IN
cord-326027-58whwspe	150	31	changes	change	NNS
cord-326027-58whwspe	150	32	in	in	IN
cord-326027-58whwspe	150	33	the	the	DT
cord-326027-58whwspe	150	34	optical	optical	JJ
cord-326027-58whwspe	150	35	section	section	NN
cord-326027-58whwspe	150	36	acquired	acquire	VBN
cord-326027-58whwspe	150	37	during	during	IN
cord-326027-58whwspe	150	38	the	the	DT
cord-326027-58whwspe	150	39	monitoring	monitoring	NN
cord-326027-58whwspe	150	40	of	of	IN
cord-326027-58whwspe	150	41	the	the	DT
cord-326027-58whwspe	150	42	infection	infection	NN
cord-326027-58whwspe	150	43	.	.	.
cord-326027-58whwspe	151	1	At	at	IN
cord-326027-58whwspe	151	2	late	late	JJ
cord-326027-58whwspe	151	3	times	time	NNS
cord-326027-58whwspe	151	4	postinfection	postinfection	NN
cord-326027-58whwspe	151	5	,	,	,
cord-326027-58whwspe	151	6	in	in	IN
cord-326027-58whwspe	151	7	most	most	JJS
cord-326027-58whwspe	151	8	of	of	IN
cord-326027-58whwspe	151	9	the	the	DT
cord-326027-58whwspe	151	10	cells	cell	NNS
cord-326027-58whwspe	151	11	,	,	,
cord-326027-58whwspe	151	12	it	-PRON-	PRP
cord-326027-58whwspe	151	13	was	be	VBD
cord-326027-58whwspe	151	14	usually	usually	RB
cord-326027-58whwspe	151	15	to	to	TO
cord-326027-58whwspe	151	16	observe	observe	VB
cord-326027-58whwspe	151	17	violent	violent	JJ
cord-326027-58whwspe	151	18	changes	change	NNS
cord-326027-58whwspe	151	19	in	in	IN
cord-326027-58whwspe	151	20	viral	viral	JJ
cord-326027-58whwspe	151	21	factories	factory	NNS
cord-326027-58whwspe	151	22	location	location	NN
cord-326027-58whwspe	151	23	(	(	-LRB-
cord-326027-58whwspe	151	24	Fig	Fig	NNP
cord-326027-58whwspe	151	25	.	.	.
cord-326027-58whwspe	151	26	6a	6a	CD
cord-326027-58whwspe	151	27	)	)	-RRB-
cord-326027-58whwspe	151	28	,	,	,
cord-326027-58whwspe	151	29	however	however	RB
cord-326027-58whwspe	151	30	these	these	DT
cord-326027-58whwspe	151	31	changes	change	NNS
cord-326027-58whwspe	151	32	seem	seem	VBP
cord-326027-58whwspe	151	33	to	to	TO
cord-326027-58whwspe	151	34	correspond	correspond	VB
cord-326027-58whwspe	151	35	to	to	IN
cord-326027-58whwspe	151	36	movements	movement	NNS
cord-326027-58whwspe	151	37	of	of	IN
cord-326027-58whwspe	151	38	infected	infected	JJ
cord-326027-58whwspe	151	39	cell	cell	NN
cord-326027-58whwspe	151	40	prior	prior	RB
cord-326027-58whwspe	151	41	to	to	IN
cord-326027-58whwspe	151	42	detachment	detachment	NN
cord-326027-58whwspe	151	43	from	from	IN
cord-326027-58whwspe	151	44	the	the	DT
cord-326027-58whwspe	151	45	dish	dish	NN
cord-326027-58whwspe	151	46	,	,	,
cord-326027-58whwspe	151	47	while	while	IN
cord-326027-58whwspe	151	48	factory	factory	NN
cord-326027-58whwspe	151	49	location	location	NN
cord-326027-58whwspe	151	50	is	be	VBZ
cord-326027-58whwspe	151	51	conserved	conserve	VBN
cord-326027-58whwspe	151	52	.	.	.
cord-326027-58whwspe	152	1	In	in	IN
cord-326027-58whwspe	152	2	another	another	DT
cord-326027-58whwspe	152	3	set	set	NN
cord-326027-58whwspe	152	4	of	of	IN
cord-326027-58whwspe	152	5	experiments	experiment	NNS
cord-326027-58whwspe	152	6	,	,	,
cord-326027-58whwspe	152	7	we	-PRON-	PRP
cord-326027-58whwspe	152	8	investigated	investigate	VBD
cord-326027-58whwspe	152	9	the	the	DT
cord-326027-58whwspe	152	10	relationship	relationship	NN
cord-326027-58whwspe	152	11	,	,	,
cord-326027-58whwspe	152	12	of	of	IN
cord-326027-58whwspe	152	13	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	152	14	status	status	NN
cord-326027-58whwspe	152	15	and	and	CC
cord-326027-58whwspe	152	16	viral	viral	JJ
cord-326027-58whwspe	152	17	factory	factory	NN
cord-326027-58whwspe	152	18	formation	formation	NN
cord-326027-58whwspe	152	19	in	in	IN
cord-326027-58whwspe	152	20	a	a	DT
cord-326027-58whwspe	152	21	time	time	NN
cord-326027-58whwspe	152	22	course	course	NN
cord-326027-58whwspe	152	23	manner	manner	NN
cord-326027-58whwspe	152	24	.	.	.
cord-326027-58whwspe	153	1	At	at	IN
cord-326027-58whwspe	153	2	initial	initial	JJ
cord-326027-58whwspe	153	3	infection	infection	NN
cord-326027-58whwspe	153	4	steps	step	VBZ
cord-326027-58whwspe	153	5	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	153	6	were	be	VBD
cord-326027-58whwspe	153	7	observed	observe	VBN
cord-326027-58whwspe	153	8	as	as	IN
cord-326027-58whwspe	153	9	an	an	DT
cord-326027-58whwspe	153	10	almost	almost	RB
cord-326027-58whwspe	153	11	continuous	continuous	JJ
cord-326027-58whwspe	153	12	succession	succession	NN
cord-326027-58whwspe	153	13	of	of	IN
cord-326027-58whwspe	153	14	organelles	organelle	NNS
cord-326027-58whwspe	153	15	extending	extend	VBG
cord-326027-58whwspe	153	16	throughout	throughout	IN
cord-326027-58whwspe	153	17	the	the	DT
cord-326027-58whwspe	153	18	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	153	19	.	.	.
cord-326027-58whwspe	154	1	Nevertheless	nevertheless	RB
cord-326027-58whwspe	154	2	,	,	,
cord-326027-58whwspe	154	3	time	time	NN
cord-326027-58whwspe	154	4	lapse	lapse	NN
cord-326027-58whwspe	154	5	experiments	experiment	NNS
cord-326027-58whwspe	154	6	with	with	IN
cord-326027-58whwspe	154	7	image	image	NN
cord-326027-58whwspe	154	8	acquisitions	acquisition	NNS
cord-326027-58whwspe	154	9	every	every	DT
cord-326027-58whwspe	154	10	5	5	CD
cord-326027-58whwspe	154	11	min	min	NN
cord-326027-58whwspe	154	12	in	in	IN
cord-326027-58whwspe	154	13	Vero	Vero	NNP
cord-326027-58whwspe	154	14	cells	cell	NNS
cord-326027-58whwspe	154	15	infected	infect	VBN
cord-326027-58whwspe	154	16	at	at	IN
cord-326027-58whwspe	154	17	a	a	DT
cord-326027-58whwspe	154	18	moi	moi	NN
cord-326027-58whwspe	154	19	of	of	IN
cord-326027-58whwspe	154	20	1	1	CD
cord-326027-58whwspe	154	21	pfu	pfu	NN
cord-326027-58whwspe	154	22	/	/	SYM
cord-326027-58whwspe	154	23	cell	cell	NN
cord-326027-58whwspe	154	24	,	,	,
cord-326027-58whwspe	154	25	showed	show	VBD
cord-326027-58whwspe	154	26	the	the	DT
cord-326027-58whwspe	154	27	progressive	progressive	JJ
cord-326027-58whwspe	154	28	alteration	alteration	NN
cord-326027-58whwspe	154	29	of	of	IN
cord-326027-58whwspe	154	30	the	the	DT
cord-326027-58whwspe	154	31	normal	normal	JJ
cord-326027-58whwspe	154	32	mitochondrial	mitochondrial	NN
cord-326027-58whwspe	154	33	pattern	pattern	NN
cord-326027-58whwspe	154	34	and	and	CC
cord-326027-58whwspe	154	35	mitochondrial	mitochondrial	JJ
cord-326027-58whwspe	154	36	irregular	irregular	JJ
cord-326027-58whwspe	154	37	clumping	clumping	NN
cord-326027-58whwspe	154	38	and	and	CC
cord-326027-58whwspe	154	39	aggregation	aggregation	NN
cord-326027-58whwspe	154	40	around	around	IN
cord-326027-58whwspe	154	41	viral	viral	JJ
cord-326027-58whwspe	154	42	factory	factory	NN
cord-326027-58whwspe	154	43	was	be	VBD
cord-326027-58whwspe	154	44	observed	observe	VBN
cord-326027-58whwspe	154	45	(	(	-LRB-
cord-326027-58whwspe	154	46	Fig	Fig	NNP
cord-326027-58whwspe	154	47	.	.	.
cord-326027-58whwspe	154	48	8)	8)	NNP
cord-326027-58whwspe	154	49	.	.	.
cord-326027-58whwspe	155	1	This	this	DT
cord-326027-58whwspe	155	2	aggregation	aggregation	NN
cord-326027-58whwspe	155	3	started	start	VBD
cord-326027-58whwspe	155	4	at	at	IN
cord-326027-58whwspe	155	5	8	8	CD
cord-326027-58whwspe	155	6	h	h	NN
cord-326027-58whwspe	155	7	after	after	IN
cord-326027-58whwspe	155	8	infection	infection	NN
cord-326027-58whwspe	155	9	,	,	,
cord-326027-58whwspe	155	10	coincident	coincident	JJ
cord-326027-58whwspe	155	11	with	with	IN
cord-326027-58whwspe	155	12	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	155	13	synthesis	synthesis	NN
cord-326027-58whwspe	155	14	,	,	,
cord-326027-58whwspe	155	15	suggesting	suggest	VBG
cord-326027-58whwspe	155	16	the	the	DT
cord-326027-58whwspe	155	17	high	high	JJ
cord-326027-58whwspe	155	18	energy	energy	NN
cord-326027-58whwspe	155	19	requirements	requirement	NNS
cord-326027-58whwspe	155	20	of	of	IN
cord-326027-58whwspe	155	21	the	the	DT
cord-326027-58whwspe	155	22	virus	virus	NN
cord-326027-58whwspe	155	23	assembly	assembly	NN
cord-326027-58whwspe	155	24	process	process	NN
cord-326027-58whwspe	155	25	.	.	.
cord-326027-58whwspe	156	1	Apoptosis	apoptosis	NN
cord-326027-58whwspe	156	2	has	have	VBZ
cord-326027-58whwspe	156	3	been	be	VBN
cord-326027-58whwspe	156	4	described	describe	VBN
cord-326027-58whwspe	156	5	to	to	TO
cord-326027-58whwspe	156	6	play	play	VB
cord-326027-58whwspe	156	7	an	an	DT
cord-326027-58whwspe	156	8	important	important	JJ
cord-326027-58whwspe	156	9	role	role	NN
cord-326027-58whwspe	156	10	in	in	IN
cord-326027-58whwspe	156	11	ASFV	ASFV	NNP
cord-326027-58whwspe	156	12	pathogenesis	pathogenesis	NN
cord-326027-58whwspe	157	1	(	(	-LRB-
cord-326027-58whwspe	157	2	Ramiro	Ramiro	NNP
cord-326027-58whwspe	157	3	-	-	HYPH
cord-326027-58whwspe	157	4	Ibanez	Ibanez	NNP
cord-326027-58whwspe	157	5	et	et	NNP
cord-326027-58whwspe	157	6	al	al	NNP
cord-326027-58whwspe	157	7	.	.	NNP
cord-326027-58whwspe	157	8	,	,	,
cord-326027-58whwspe	157	9	1996	1996	CD
cord-326027-58whwspe	157	10	)	)	-RRB-
cord-326027-58whwspe	157	11	,	,	,
cord-326027-58whwspe	157	12	so	so	RB
cord-326027-58whwspe	157	13	we	-PRON-	PRP
cord-326027-58whwspe	157	14	decided	decide	VBD
cord-326027-58whwspe	157	15	to	to	TO
cord-326027-58whwspe	157	16	analyze	analyze	VB
cord-326027-58whwspe	157	17	the	the	DT
cord-326027-58whwspe	157	18	very	very	RB
cord-326027-58whwspe	157	19	late	late	JJ
cord-326027-58whwspe	157	20	stages	stage	NNS
cord-326027-58whwspe	157	21	of	of	IN
cord-326027-58whwspe	157	22	ASFV	ASFV	NNP
cord-326027-58whwspe	157	23	infection	infection	NN
cord-326027-58whwspe	157	24	in	in	IN
cord-326027-58whwspe	157	25	living	living	NN
cord-326027-58whwspe	157	26	cells	cell	NNS
cord-326027-58whwspe	157	27	infected	infect	VBN
cord-326027-58whwspe	157	28	with	with	IN
cord-326027-58whwspe	157	29	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	157	30	.	.	.
cord-326027-58whwspe	158	1	Fig	Fig	NNP
cord-326027-58whwspe	158	2	.	.	NNP
cord-326027-58whwspe	158	3	7	7	CD
cord-326027-58whwspe	158	4	shows	show	NNS
cord-326027-58whwspe	158	5	in	in	IN
cord-326027-58whwspe	158	6	detail	detail	NN
cord-326027-58whwspe	158	7	the	the	DT
cord-326027-58whwspe	158	8	characteristic	characteristic	JJ
cord-326027-58whwspe	158	9	morphological	morphological	JJ
cord-326027-58whwspe	158	10	changes	change	NNS
cord-326027-58whwspe	158	11	that	that	WDT
cord-326027-58whwspe	158	12	occur	occur	VBP
cord-326027-58whwspe	158	13	during	during	IN
cord-326027-58whwspe	158	14	programmed	program	VBN
cord-326027-58whwspe	158	15	cell	cell	NN
cord-326027-58whwspe	158	16	death	death	NN
cord-326027-58whwspe	158	17	of	of	IN
cord-326027-58whwspe	158	18	an	an	DT
cord-326027-58whwspe	158	19	infected	infected	JJ
cord-326027-58whwspe	158	20	cell	cell	NN
cord-326027-58whwspe	158	21	.	.	.
cord-326027-58whwspe	159	1	Since	since	IN
cord-326027-58whwspe	159	2	12	12	CD
cord-326027-58whwspe	159	3	hpi	hpi	NNS
cord-326027-58whwspe	159	4	an	an	DT
cord-326027-58whwspe	159	5	early	early	JJ
cord-326027-58whwspe	159	6	cytopathic	cytopathic	JJ
cord-326027-58whwspe	159	7	effect	effect	NN
cord-326027-58whwspe	159	8	,	,	,
cord-326027-58whwspe	159	9	consisting	consist	VBG
cord-326027-58whwspe	159	10	in	in	IN
cord-326027-58whwspe	159	11	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	159	12	vacuolization	vacuolization	NN
cord-326027-58whwspe	159	13	,	,	,
cord-326027-58whwspe	159	14	rounding	rounding	NN
cord-326027-58whwspe	159	15	and	and	CC
cord-326027-58whwspe	159	16	reduction	reduction	NN
cord-326027-58whwspe	159	17	in	in	IN
cord-326027-58whwspe	159	18	the	the	DT
cord-326027-58whwspe	159	19	size	size	NN
cord-326027-58whwspe	159	20	of	of	IN
cord-326027-58whwspe	159	21	the	the	DT
cord-326027-58whwspe	159	22	infected	infected	JJ
cord-326027-58whwspe	159	23	cell	cell	NN
cord-326027-58whwspe	159	24	was	be	VBD
cord-326027-58whwspe	159	25	be	be	VB
cord-326027-58whwspe	159	26	observed	observe	VBN
cord-326027-58whwspe	159	27	.	.	.
cord-326027-58whwspe	160	1	From	from	IN
cord-326027-58whwspe	160	2	this	this	DT
cord-326027-58whwspe	160	3	time	time	NN
cord-326027-58whwspe	160	4	point	point	NN
cord-326027-58whwspe	160	5	onwards	onwards	RB
cord-326027-58whwspe	160	6	,	,	,
cord-326027-58whwspe	160	7	the	the	DT
cord-326027-58whwspe	160	8	cell	cell	NN
cord-326027-58whwspe	160	9	lost	lose	VBD
cord-326027-58whwspe	160	10	contacts	contact	NNS
cord-326027-58whwspe	160	11	with	with	IN
cord-326027-58whwspe	160	12	neighboring	neighboring	NN
cord-326027-58whwspe	160	13	cells	cell	NNS
cord-326027-58whwspe	160	14	and	and	CC
cord-326027-58whwspe	160	15	detached	detach	VBN
cord-326027-58whwspe	160	16	from	from	IN
cord-326027-58whwspe	160	17	the	the	DT
cord-326027-58whwspe	160	18	monolayer	monolayer	NN
cord-326027-58whwspe	160	19	,	,	,
cord-326027-58whwspe	160	20	undergoing	undergo	VBG
cord-326027-58whwspe	160	21	nuclear	nuclear	JJ
cord-326027-58whwspe	160	22	fragmentation	fragmentation	NN
cord-326027-58whwspe	160	23	and	and	CC
cord-326027-58whwspe	160	24	final	final	JJ
cord-326027-58whwspe	160	25	cell	cell	NN
cord-326027-58whwspe	160	26	death	death	NN
cord-326027-58whwspe	160	27	.	.	.
cord-326027-58whwspe	161	1	This	this	DT
cord-326027-58whwspe	161	2	process	process	NN
cord-326027-58whwspe	161	3	included	include	VBD
cord-326027-58whwspe	161	4	typical	typical	JJ
cord-326027-58whwspe	161	5	membrane	membrane	NN
cord-326027-58whwspe	161	6	blebbing	blebbe	VBG
cord-326027-58whwspe	161	7	of	of	IN
cord-326027-58whwspe	161	8	the	the	DT
cord-326027-58whwspe	161	9	infected	infected	JJ
cord-326027-58whwspe	161	10	cell	cell	NN
cord-326027-58whwspe	161	11	that	that	WDT
cord-326027-58whwspe	161	12	led	lead	VBD
cord-326027-58whwspe	161	13	to	to	IN
cord-326027-58whwspe	161	14	the	the	DT
cord-326027-58whwspe	161	15	formation	formation	NN
cord-326027-58whwspe	161	16	of	of	IN
cord-326027-58whwspe	161	17	numerous	numerous	JJ
cord-326027-58whwspe	161	18	vesicles	vesicle	NNS
cord-326027-58whwspe	161	19	containing	contain	VBG
cord-326027-58whwspe	161	20	large	large	JJ
cord-326027-58whwspe	161	21	amounts	amount	NNS
cord-326027-58whwspe	161	22	of	of	IN
cord-326027-58whwspe	161	23	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	161	24	.	.	.
cord-326027-58whwspe	162	1	Vesicle	Vesicle	NNP
cord-326027-58whwspe	162	2	formation	formation	NN
cord-326027-58whwspe	162	3	has	have	VBZ
cord-326027-58whwspe	162	4	been	be	VBN
cord-326027-58whwspe	162	5	considered	consider	VBN
cord-326027-58whwspe	162	6	a	a	DT
cord-326027-58whwspe	162	7	very	very	RB
cord-326027-58whwspe	162	8	characteristic	characteristic	JJ
cord-326027-58whwspe	162	9	feature	feature	NN
cord-326027-58whwspe	162	10	of	of	IN
cord-326027-58whwspe	162	11	ASFV	ASFV	NNP
cord-326027-58whwspe	162	12	infection	infection	NN
cord-326027-58whwspe	162	13	in	in	IN
cord-326027-58whwspe	162	14	cultured	cultured	JJ
cord-326027-58whwspe	162	15	cells	cell	NNS
cord-326027-58whwspe	162	16	and	and	CC
cord-326027-58whwspe	162	17	these	these	DT
cord-326027-58whwspe	162	18	are	be	VBP
cord-326027-58whwspe	162	19	viruscontaining	viruscontaine	VBG
cord-326027-58whwspe	162	20	vesicles	vesicle	NNS
cord-326027-58whwspe	162	21	.	.	.
cord-326027-58whwspe	163	1	Completion	completion	NN
cord-326027-58whwspe	163	2	of	of	IN
cord-326027-58whwspe	163	3	the	the	DT
cord-326027-58whwspe	163	4	process	process	NN
cord-326027-58whwspe	163	5	from	from	IN
cord-326027-58whwspe	163	6	the	the	DT
cord-326027-58whwspe	163	7	first	first	JJ
cord-326027-58whwspe	163	8	morphological	morphological	JJ
cord-326027-58whwspe	163	9	features	feature	NNS
cord-326027-58whwspe	163	10	of	of	IN
cord-326027-58whwspe	163	11	apoptosis	apoptosis	NN
cord-326027-58whwspe	163	12	lasted	last	VBD
cord-326027-58whwspe	163	13	around	around	RB
cord-326027-58whwspe	163	14	6	6	CD
cord-326027-58whwspe	163	15	h.	h.	NN
cord-326027-58whwspe	163	16	Successful	successful	JJ
cord-326027-58whwspe	163	17	African	african	JJ
cord-326027-58whwspe	163	18	swine	swine	NN
cord-326027-58whwspe	163	19	fever	fever	NN
cord-326027-58whwspe	163	20	virus	virus	NN
cord-326027-58whwspe	163	21	life	life	NN
cord-326027-58whwspe	163	22	cycle	cycle	NN
cord-326027-58whwspe	163	23	is	be	VBZ
cord-326027-58whwspe	163	24	dependent	dependent	JJ
cord-326027-58whwspe	163	25	on	on	IN
cord-326027-58whwspe	163	26	several	several	JJ
cord-326027-58whwspe	163	27	and	and	CC
cord-326027-58whwspe	163	28	critical	critical	JJ
cord-326027-58whwspe	163	29	interactions	interaction	NNS
cord-326027-58whwspe	163	30	with	with	IN
cord-326027-58whwspe	163	31	host	host	NN
cord-326027-58whwspe	163	32	cell	cell	NN
cord-326027-58whwspe	163	33	which	which	WDT
cord-326027-58whwspe	163	34	remain	remain	VBP
cord-326027-58whwspe	163	35	poorly	poorly	RB
cord-326027-58whwspe	163	36	understood	understand	VBN
cord-326027-58whwspe	163	37	.	.	.
cord-326027-58whwspe	164	1	Some	some	DT
cord-326027-58whwspe	164	2	of	of	IN
cord-326027-58whwspe	164	3	these	these	DT
cord-326027-58whwspe	164	4	events	event	NNS
cord-326027-58whwspe	164	5	include	include	VBP
cord-326027-58whwspe	164	6	virus	virus	NN
cord-326027-58whwspe	164	7	internalization	internalization	NN
cord-326027-58whwspe	164	8	into	into	IN
cord-326027-58whwspe	164	9	cellular	cellular	JJ
cord-326027-58whwspe	164	10	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	164	11	,	,	,
cord-326027-58whwspe	164	12	transport	transport	NN
cord-326027-58whwspe	164	13	to	to	IN
cord-326027-58whwspe	164	14	perinuclear	perinuclear	NN
cord-326027-58whwspe	164	15	areas	area	NNS
cord-326027-58whwspe	164	16	where	where	WRB
cord-326027-58whwspe	164	17	viral	viral	JJ
cord-326027-58whwspe	164	18	DNA	dna	NN
cord-326027-58whwspe	164	19	replication	replication	NN
cord-326027-58whwspe	164	20	occurs	occur	VBZ
cord-326027-58whwspe	164	21	,	,	,
cord-326027-58whwspe	164	22	egress	egress	NN
cord-326027-58whwspe	164	23	of	of	IN
cord-326027-58whwspe	164	24	viral	viral	JJ
cord-326027-58whwspe	164	25	progeny	progeny	NN
cord-326027-58whwspe	164	26	from	from	IN
cord-326027-58whwspe	164	27	assembly	assembly	NN
cord-326027-58whwspe	164	28	sites	site	NNS
cord-326027-58whwspe	164	29	to	to	IN
cord-326027-58whwspe	164	30	cell	cell	NN
cord-326027-58whwspe	164	31	periphery	periphery	NN
cord-326027-58whwspe	164	32	and	and	CC
cord-326027-58whwspe	164	33	virus	virus	NN
cord-326027-58whwspe	164	34	induced	induce	VBN
cord-326027-58whwspe	164	35	cell	cell	NN
cord-326027-58whwspe	164	36	death	death	NN
cord-326027-58whwspe	164	37	.	.	.
cord-326027-58whwspe	165	1	Recent	recent	JJ
cord-326027-58whwspe	165	2	improvements	improvement	NNS
cord-326027-58whwspe	165	3	in	in	IN
cord-326027-58whwspe	165	4	optical	optical	JJ
cord-326027-58whwspe	165	5	imaging	imaging	NN
cord-326027-58whwspe	165	6	techniques	technique	NNS
cord-326027-58whwspe	165	7	combined	combine	VBN
cord-326027-58whwspe	165	8	with	with	IN
cord-326027-58whwspe	165	9	protein	protein	NN
cord-326027-58whwspe	165	10	fusion	fusion	NN
cord-326027-58whwspe	165	11	to	to	IN
cord-326027-58whwspe	165	12	GFP	GFP	NNP
cord-326027-58whwspe	165	13	variants	variant	NNS
cord-326027-58whwspe	165	14	,	,	,
cord-326027-58whwspe	165	15	open	open	VB
cord-326027-58whwspe	165	16	new	new	JJ
cord-326027-58whwspe	165	17	Fig	fig	NN
cord-326027-58whwspe	165	18	.	.	.
cord-326027-58whwspe	166	1	5	5	LS
cord-326027-58whwspe	166	2	.	.	.
cord-326027-58whwspe	167	1	Analysis	analysis	NN
cord-326027-58whwspe	167	2	of	of	IN
cord-326027-58whwspe	167	3	the	the	DT
cord-326027-58whwspe	167	4	intracellular	intracellular	JJ
cord-326027-58whwspe	167	5	movement	movement	NN
cord-326027-58whwspe	167	6	of	of	IN
cord-326027-58whwspe	167	7	B54GFP-2	b54gfp-2	JJ
cord-326027-58whwspe	167	8	viral	viral	JJ
cord-326027-58whwspe	167	9	particles	particle	NNS
cord-326027-58whwspe	167	10	.	.	.
cord-326027-58whwspe	168	1	Vero	Vero	NNP
cord-326027-58whwspe	168	2	cells	cell	NNS
cord-326027-58whwspe	168	3	were	be	VBD
cord-326027-58whwspe	168	4	B54GFP-2	b54gfp-2	RB
cord-326027-58whwspe	168	5	infected	infect	VBN
cord-326027-58whwspe	168	6	with	with	IN
cord-326027-58whwspe	168	7	20	20	CD
cord-326027-58whwspe	168	8	pfu	pfu	NN
cord-326027-58whwspe	168	9	/	/	SYM
cord-326027-58whwspe	168	10	cell	cell	NN
cord-326027-58whwspe	168	11	and	and	CC
cord-326027-58whwspe	168	12	examined	examine	VBN
cord-326027-58whwspe	168	13	by	by	IN
cord-326027-58whwspe	168	14	confocal	confocal	JJ
cord-326027-58whwspe	168	15	microscopy	microscopy	NNS
cord-326027-58whwspe	168	16	at	at	IN
cord-326027-58whwspe	168	17	1	1	CD
cord-326027-58whwspe	168	18	hpi	hpi	NNP
cord-326027-58whwspe	168	19	,	,	,
cord-326027-58whwspe	168	20	acquiring	acquire	VBG
cord-326027-58whwspe	168	21	images	image	NNS
cord-326027-58whwspe	168	22	every	every	DT
cord-326027-58whwspe	168	23	3	3	CD
cord-326027-58whwspe	168	24	s	s	NN
cord-326027-58whwspe	168	25	corresponding	correspond	VBG
cord-326027-58whwspe	168	26	to	to	IN
cord-326027-58whwspe	168	27	0.5	0.5	CD
cord-326027-58whwspe	168	28	μm	μm	NN
cord-326027-58whwspe	168	29	optical	optical	JJ
cord-326027-58whwspe	168	30	sections	section	NNS
cord-326027-58whwspe	168	31	from	from	IN
cord-326027-58whwspe	168	32	the	the	DT
cord-326027-58whwspe	168	33	Z	z	NN
cord-326027-58whwspe	168	34	axis	axis	NN
cord-326027-58whwspe	168	35	.	.	.
cord-326027-58whwspe	169	1	Virus	virus	NN
cord-326027-58whwspe	169	2	particles	particle	NNS
cord-326027-58whwspe	169	3	were	be	VBD
cord-326027-58whwspe	169	4	detected	detect	VBN
cord-326027-58whwspe	169	5	as	as	IN
cord-326027-58whwspe	169	6	EGFP	EGFP	NNP
cord-326027-58whwspe	169	7	point	point	NN
cord-326027-58whwspe	169	8	sources	source	NNS
cord-326027-58whwspe	169	9	,	,	,
cord-326027-58whwspe	169	10	and	and	CC
cord-326027-58whwspe	169	11	correspondent	correspondent	NN
cord-326027-58whwspe	169	12	transmitted	transmit	VBD
cord-326027-58whwspe	169	13	light	light	JJ
cord-326027-58whwspe	169	14	image	image	NN
cord-326027-58whwspe	169	15	provided	provide	VBD
cord-326027-58whwspe	169	16	points	point	NNS
cord-326027-58whwspe	169	17	of	of	IN
cord-326027-58whwspe	169	18	reference	reference	NN
cord-326027-58whwspe	169	19	information	information	NN
cord-326027-58whwspe	169	20	.	.	.
cord-326027-58whwspe	170	1	Movement	movement	NN
cord-326027-58whwspe	170	2	of	of	IN
cord-326027-58whwspe	170	3	10	10	CD
cord-326027-58whwspe	170	4	virions	virion	NNS
cord-326027-58whwspe	170	5	were	be	VBD
cord-326027-58whwspe	170	6	individually	individually	RB
cord-326027-58whwspe	170	7	analyzed	analyze	VBN
cord-326027-58whwspe	170	8	.	.	.
cord-326027-58whwspe	171	1	Upper	upper	JJ
cord-326027-58whwspe	171	2	image	image	NN
cord-326027-58whwspe	171	3	shows	show	VBZ
cord-326027-58whwspe	171	4	an	an	DT
cord-326027-58whwspe	171	5	example	example	NN
cord-326027-58whwspe	171	6	of	of	IN
cord-326027-58whwspe	171	7	the	the	DT
cord-326027-58whwspe	171	8	trajectory	trajectory	NN
cord-326027-58whwspe	171	9	followed	follow	VBN
cord-326027-58whwspe	171	10	by	by	IN
cord-326027-58whwspe	171	11	one	one	CD
cord-326027-58whwspe	171	12	of	of	IN
cord-326027-58whwspe	171	13	the	the	DT
cord-326027-58whwspe	171	14	virions	virion	NNS
cord-326027-58whwspe	171	15	analyzed	analyze	VBN
cord-326027-58whwspe	171	16	(	(	-LRB-
cord-326027-58whwspe	171	17	white	white	JJ
cord-326027-58whwspe	171	18	discontinuous	discontinuous	JJ
cord-326027-58whwspe	171	19	line	line	NN
cord-326027-58whwspe	171	20	)	)	-RRB-
cord-326027-58whwspe	171	21	and	and	CC
cord-326027-58whwspe	171	22	selected	select	VBN
cord-326027-58whwspe	171	23	area	area	NN
cord-326027-58whwspe	171	24	is	be	VBZ
cord-326027-58whwspe	171	25	enlarged	enlarge	VBN
cord-326027-58whwspe	171	26	below	below	RB
cord-326027-58whwspe	171	27	.	.	.
cord-326027-58whwspe	172	1	Total	total	JJ
cord-326027-58whwspe	172	2	distance	distance	NN
cord-326027-58whwspe	172	3	covered	cover	VBN
cord-326027-58whwspe	172	4	by	by	IN
cord-326027-58whwspe	172	5	this	this	DT
cord-326027-58whwspe	172	6	virion	virion	NN
cord-326027-58whwspe	172	7	was	be	VBD
cord-326027-58whwspe	172	8	11.74	11.74	CD
cord-326027-58whwspe	172	9	μm	μm	NNS
cord-326027-58whwspe	172	10	in	in	IN
cord-326027-58whwspe	172	11	30	30	CD
cord-326027-58whwspe	172	12	s	s	NN
cord-326027-58whwspe	172	13	,	,	,
cord-326027-58whwspe	172	14	with	with	IN
cord-326027-58whwspe	172	15	an	an	DT
cord-326027-58whwspe	172	16	average	average	JJ
cord-326027-58whwspe	172	17	speed	speed	NN
cord-326027-58whwspe	172	18	of	of	IN
cord-326027-58whwspe	172	19	0.5	0.5	CD
cord-326027-58whwspe	172	20	μm	μm	NN
cord-326027-58whwspe	172	21	/	/	SYM
cord-326027-58whwspe	172	22	s	s	NN
cord-326027-58whwspe	172	23	.	.	.
cord-326027-58whwspe	173	1	As	as	IN
cord-326027-58whwspe	173	2	can	can	MD
cord-326027-58whwspe	173	3	be	be	VB
cord-326027-58whwspe	173	4	observed	observe	VBN
cord-326027-58whwspe	173	5	in	in	IN
cord-326027-58whwspe	173	6	the	the	DT
cord-326027-58whwspe	173	7	figure	figure	NN
cord-326027-58whwspe	173	8	,	,	,
cord-326027-58whwspe	173	9	virion	virion	NN
cord-326027-58whwspe	173	10	velocity	velocity	NN
cord-326027-58whwspe	173	11	was	be	VBD
cord-326027-58whwspe	173	12	not	not	RB
cord-326027-58whwspe	173	13	constant	constant	JJ
cord-326027-58whwspe	173	14	,	,	,
cord-326027-58whwspe	173	15	with	with	IN
cord-326027-58whwspe	173	16	a	a	DT
cord-326027-58whwspe	173	17	maximum	maximum	JJ
cord-326027-58whwspe	173	18	speed	speed	NN
cord-326027-58whwspe	173	19	from	from	IN
cord-326027-58whwspe	173	20	12	12	CD
cord-326027-58whwspe	173	21	to	to	TO
cord-326027-58whwspe	173	22	15	15	CD
cord-326027-58whwspe	173	23	s.	s.	NNP
cord-326027-58whwspe	173	24	Scale	Scale	NNP
cord-326027-58whwspe	173	25	bar	bar	NN
cord-326027-58whwspe	173	26	,	,	,
cord-326027-58whwspe	173	27	8μm	8μm	NN
cord-326027-58whwspe	173	28	as	as	IN
cord-326027-58whwspe	173	29	indicated	indicate	VBN
cord-326027-58whwspe	173	30	.	.	.
cord-326027-58whwspe	174	1	possibilities	possibility	NNS
cord-326027-58whwspe	174	2	for	for	IN
cord-326027-58whwspe	174	3	the	the	DT
cord-326027-58whwspe	174	4	direct	direct	JJ
cord-326027-58whwspe	174	5	analysis	analysis	NN
cord-326027-58whwspe	174	6	of	of	IN
cord-326027-58whwspe	174	7	these	these	DT
cord-326027-58whwspe	174	8	dynamic	dynamic	JJ
cord-326027-58whwspe	174	9	processes	process	NNS
cord-326027-58whwspe	174	10	.	.	.
cord-326027-58whwspe	175	1	This	this	DT
cord-326027-58whwspe	175	2	new	new	JJ
cord-326027-58whwspe	175	3	methodology	methodology	NN
cord-326027-58whwspe	175	4	has	have	VBZ
cord-326027-58whwspe	175	5	been	be	VBN
cord-326027-58whwspe	175	6	successfully	successfully	RB
cord-326027-58whwspe	175	7	applied	apply	VBN
cord-326027-58whwspe	175	8	to	to	TO
cord-326027-58whwspe	175	9	study	study	VB
cord-326027-58whwspe	175	10	different	different	JJ
cord-326027-58whwspe	175	11	aspects	aspect	NNS
cord-326027-58whwspe	175	12	of	of	IN
cord-326027-58whwspe	175	13	the	the	DT
cord-326027-58whwspe	175	14	infection	infection	NN
cord-326027-58whwspe	175	15	of	of	IN
cord-326027-58whwspe	175	16	adenoviruses	adenovirus	NNS
cord-326027-58whwspe	175	17	(	(	-LRB-
cord-326027-58whwspe	175	18	Lux	Lux	NNP
cord-326027-58whwspe	175	19	et	et	NNP
cord-326027-58whwspe	175	20	al	al	NNP
cord-326027-58whwspe	175	21	.	.	.
cord-326027-58whwspe	176	1	,	,	,
cord-326027-58whwspe	176	2	2005	2005	CD
cord-326027-58whwspe	176	3	)	)	-RRB-
cord-326027-58whwspe	176	4	,	,	,
cord-326027-58whwspe	176	5	herpesviruses	herpesviruses	FW
cord-326027-58whwspe	176	6	(	(	-LRB-
cord-326027-58whwspe	176	7	Glotzer	Glotzer	NNP
cord-326027-58whwspe	176	8	et	et	NNP
cord-326027-58whwspe	176	9	al	al	NNP
cord-326027-58whwspe	176	10	.	.	NNP
cord-326027-58whwspe	176	11	,	,	,
cord-326027-58whwspe	176	12	2001	2001	CD
cord-326027-58whwspe	176	13	;	;	:
cord-326027-58whwspe	177	1	Sampaio	Sampaio	NNP
cord-326027-58whwspe	177	2	et	et	NNP
cord-326027-58whwspe	177	3	al	al	NNP
cord-326027-58whwspe	177	4	.	.	NNP
cord-326027-58whwspe	177	5	,	,	,
cord-326027-58whwspe	177	6	2005	2005	CD
cord-326027-58whwspe	177	7	)	)	-RRB-
cord-326027-58whwspe	177	8	,	,	,
cord-326027-58whwspe	177	9	poxviruses	poxvirus	NNS
cord-326027-58whwspe	177	10	(	(	-LRB-
cord-326027-58whwspe	178	1	Geada	Geada	NNP
cord-326027-58whwspe	178	2	et	et	FW
cord-326027-58whwspe	178	3	al	al	NNP
cord-326027-58whwspe	178	4	.	.	.
cord-326027-58whwspe	179	1	,	,	,
cord-326027-58whwspe	179	2	2001	2001	CD
cord-326027-58whwspe	179	3	;	;	:
cord-326027-58whwspe	179	4	Hollinshead	Hollinshead	NNP
cord-326027-58whwspe	179	5	et	et	NNP
cord-326027-58whwspe	179	6	al	al	NNP
cord-326027-58whwspe	179	7	.	.	NNP
cord-326027-58whwspe	179	8	,	,	,
cord-326027-58whwspe	179	9	2001	2001	CD
cord-326027-58whwspe	179	10	;	;	:
cord-326027-58whwspe	179	11	Ward	Ward	NNP
cord-326027-58whwspe	179	12	and	and	CC
cord-326027-58whwspe	179	13	Moss	Moss	NNP
cord-326027-58whwspe	179	14	,	,	,
cord-326027-58whwspe	179	15	2001	2001	CD
cord-326027-58whwspe	179	16	)	)	-RRB-
cord-326027-58whwspe	180	1	,	,	,
cord-326027-58whwspe	180	2	rhabdoviruses	rhabdoviruses	NNP
cord-326027-58whwspe	180	3	(	(	-LRB-
cord-326027-58whwspe	180	4	Finke	Finke	NNP
cord-326027-58whwspe	180	5	et	et	NNP
cord-326027-58whwspe	180	6	al	al	NNP
cord-326027-58whwspe	180	7	.	.	NNP
cord-326027-58whwspe	180	8	,	,	,
cord-326027-58whwspe	180	9	2004	2004	CD
cord-326027-58whwspe	180	10	)	)	-RRB-
cord-326027-58whwspe	180	11	or	or	CC
cord-326027-58whwspe	180	12	retroviruses	retrovirus	NNS
cord-326027-58whwspe	180	13	(	(	-LRB-
cord-326027-58whwspe	180	14	McDonald	McDonald	NNP
cord-326027-58whwspe	180	15	et	et	NNP
cord-326027-58whwspe	180	16	al	al	NNP
cord-326027-58whwspe	180	17	.	.	NNP
cord-326027-58whwspe	180	18	,	,	,
cord-326027-58whwspe	180	19	2002	2002	CD
cord-326027-58whwspe	180	20	;	;	:
cord-326027-58whwspe	180	21	Muller	Muller	NNP
cord-326027-58whwspe	180	22	et	et	NNP
cord-326027-58whwspe	180	23	al	al	NNP
cord-326027-58whwspe	180	24	.	.	NNP
cord-326027-58whwspe	180	25	,	,	,
cord-326027-58whwspe	180	26	2004	2004	CD
cord-326027-58whwspe	180	27	)	)	-RRB-
cord-326027-58whwspe	180	28	.	.	.
cord-326027-58whwspe	181	1	We	-PRON-	PRP
cord-326027-58whwspe	181	2	reported	report	VBD
cord-326027-58whwspe	181	3	here	here	RB
cord-326027-58whwspe	181	4	the	the	DT
cord-326027-58whwspe	181	5	generation	generation	NN
cord-326027-58whwspe	181	6	of	of	IN
cord-326027-58whwspe	181	7	a	a	DT
cord-326027-58whwspe	181	8	fluorescently	fluorescently	RB
cord-326027-58whwspe	181	9	tagged	tag	VBN
cord-326027-58whwspe	181	10	and	and	CC
cord-326027-58whwspe	181	11	viable	viable	JJ
cord-326027-58whwspe	181	12	ASFV	ASFV	NNP
cord-326027-58whwspe	181	13	expressing	express	VBG
cord-326027-58whwspe	181	14	the	the	DT
cord-326027-58whwspe	181	15	structural	structural	JJ
cord-326027-58whwspe	181	16	protein	protein	NN
cord-326027-58whwspe	181	17	p54	p54	NN
cord-326027-58whwspe	181	18	as	as	IN
cord-326027-58whwspe	181	19	an	an	DT
cord-326027-58whwspe	181	20	EGFP	EGFP	NNP
cord-326027-58whwspe	181	21	fusion	fusion	NN
cord-326027-58whwspe	181	22	protein	protein	NN
cord-326027-58whwspe	181	23	.	.	.
cord-326027-58whwspe	182	1	Expression	expression	NN
cord-326027-58whwspe	182	2	of	of	IN
cord-326027-58whwspe	182	3	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	182	4	enables	enable	VBZ
cord-326027-58whwspe	182	5	easy	easy	JJ
cord-326027-58whwspe	182	6	identification	identification	NN
cord-326027-58whwspe	182	7	of	of	IN
cord-326027-58whwspe	182	8	infected	infected	JJ
cord-326027-58whwspe	182	9	cells	cell	NNS
cord-326027-58whwspe	182	10	by	by	IN
cord-326027-58whwspe	182	11	direct	direct	JJ
cord-326027-58whwspe	182	12	fluorescence	fluorescence	NN
cord-326027-58whwspe	182	13	analysis	analysis	NN
cord-326027-58whwspe	182	14	,	,	,
cord-326027-58whwspe	182	15	which	which	WDT
cord-326027-58whwspe	182	16	results	result	VBZ
cord-326027-58whwspe	182	17	useful	useful	JJ
cord-326027-58whwspe	182	18	when	when	WRB
cord-326027-58whwspe	182	19	immunodetection	immunodetection	NN
cord-326027-58whwspe	182	20	of	of	IN
cord-326027-58whwspe	182	21	diverse	diverse	JJ
cord-326027-58whwspe	182	22	antigens	antigen	NNS
cord-326027-58whwspe	182	23	in	in	IN
cord-326027-58whwspe	182	24	the	the	DT
cord-326027-58whwspe	182	25	same	same	JJ
cord-326027-58whwspe	182	26	sample	sample	NN
cord-326027-58whwspe	182	27	is	be	VBZ
cord-326027-58whwspe	182	28	desirable	desirable	JJ
cord-326027-58whwspe	182	29	.	.	.
cord-326027-58whwspe	183	1	Selection	selection	NN
cord-326027-58whwspe	183	2	of	of	IN
cord-326027-58whwspe	183	3	p54	p54	NN
cord-326027-58whwspe	183	4	as	as	IN
cord-326027-58whwspe	183	5	the	the	DT
cord-326027-58whwspe	183	6	viral	viral	JJ
cord-326027-58whwspe	183	7	fusion	fusion	NN
cord-326027-58whwspe	183	8	protein	protein	NN
cord-326027-58whwspe	183	9	was	be	VBD
cord-326027-58whwspe	183	10	supported	support	VBN
cord-326027-58whwspe	183	11	by	by	IN
cord-326027-58whwspe	183	12	several	several	JJ
cord-326027-58whwspe	183	13	reasons	reason	NNS
cord-326027-58whwspe	183	14	.	.	.
cord-326027-58whwspe	184	1	Since	since	IN
cord-326027-58whwspe	184	2	p54	p54	NN
cord-326027-58whwspe	184	3	is	be	VBZ
cord-326027-58whwspe	184	4	located	locate	VBN
cord-326027-58whwspe	184	5	in	in	IN
cord-326027-58whwspe	184	6	the	the	DT
cord-326027-58whwspe	184	7	inner	inner	JJ
cord-326027-58whwspe	184	8	viral	viral	JJ
cord-326027-58whwspe	184	9	envelope	envelope	NN
cord-326027-58whwspe	184	10	(	(	-LRB-
cord-326027-58whwspe	184	11	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	184	12	et	et	NNP
cord-326027-58whwspe	184	13	al	al	NNP
cord-326027-58whwspe	184	14	.	.	NNP
cord-326027-58whwspe	184	15	,	,	,
cord-326027-58whwspe	184	16	1994	1994	CD
cord-326027-58whwspe	184	17	)	)	-RRB-
cord-326027-58whwspe	184	18	,	,	,
cord-326027-58whwspe	184	19	fluorescent	fluorescent	NN
cord-326027-58whwspe	184	20	p54	p54	NN
cord-326027-58whwspe	184	21	would	would	MD
cord-326027-58whwspe	184	22	allow	allow	VB
cord-326027-58whwspe	184	23	the	the	DT
cord-326027-58whwspe	184	24	detection	detection	NN
cord-326027-58whwspe	184	25	of	of	IN
cord-326027-58whwspe	184	26	individual	individual	JJ
cord-326027-58whwspe	184	27	viral	viral	JJ
cord-326027-58whwspe	184	28	particles	particle	NNS
cord-326027-58whwspe	184	29	upon	upon	IN
cord-326027-58whwspe	184	30	infection	infection	NN
cord-326027-58whwspe	184	31	.	.	.
cord-326027-58whwspe	185	1	As	as	IN
cord-326027-58whwspe	185	2	the	the	DT
cord-326027-58whwspe	185	3	carboxi	carboxi	NN
cord-326027-58whwspe	185	4	-	-	HYPH
cord-326027-58whwspe	185	5	terminal	terminal	NN
cord-326027-58whwspe	185	6	tail	tail	NN
cord-326027-58whwspe	185	7	of	of	IN
cord-326027-58whwspe	185	8	p54	p54	NN
cord-326027-58whwspe	185	9	is	be	VBZ
cord-326027-58whwspe	185	10	facing	face	VBG
cord-326027-58whwspe	185	11	the	the	DT
cord-326027-58whwspe	185	12	surface	surface	NN
cord-326027-58whwspe	185	13	of	of	IN
cord-326027-58whwspe	185	14	mature	mature	JJ
cord-326027-58whwspe	185	15	virions	virion	NNS
cord-326027-58whwspe	185	16	,	,	,
cord-326027-58whwspe	185	17	it	-PRON-	PRP
cord-326027-58whwspe	185	18	was	be	VBD
cord-326027-58whwspe	185	19	expected	expect	VBN
cord-326027-58whwspe	185	20	to	to	TO
cord-326027-58whwspe	185	21	be	be	VB
cord-326027-58whwspe	185	22	a	a	DT
cord-326027-58whwspe	185	23	suitable	suitable	JJ
cord-326027-58whwspe	185	24	target	target	NN
cord-326027-58whwspe	185	25	for	for	IN
cord-326027-58whwspe	185	26	EGFP	EGFP	NNP
cord-326027-58whwspe	185	27	fusion	fusion	NN
cord-326027-58whwspe	185	28	.	.	.
cord-326027-58whwspe	186	1	On	on	IN
cord-326027-58whwspe	186	2	the	the	DT
cord-326027-58whwspe	186	3	other	other	JJ
cord-326027-58whwspe	186	4	hand	hand	NN
cord-326027-58whwspe	186	5	,	,	,
cord-326027-58whwspe	186	6	newly	newly	RB
cord-326027-58whwspe	186	7	synthesized	synthesize	VBN
cord-326027-58whwspe	186	8	p54	p54	NN
cord-326027-58whwspe	186	9	accumulates	accumulate	NNS
cord-326027-58whwspe	186	10	within	within	IN
cord-326027-58whwspe	186	11	perinuclear	perinuclear	NNP
cord-326027-58whwspe	186	12	areas	area	NNS
cord-326027-58whwspe	186	13	where	where	WRB
cord-326027-58whwspe	186	14	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	186	15	takes	take	VBZ
cord-326027-58whwspe	186	16	place	place	NN
cord-326027-58whwspe	186	17	at	at	IN
cord-326027-58whwspe	186	18	late	late	JJ
cord-326027-58whwspe	186	19	times	time	NNS
cord-326027-58whwspe	186	20	postinfection	postinfection	NN
cord-326027-58whwspe	186	21	.	.	.
cord-326027-58whwspe	187	1	Thus	thus	RB
cord-326027-58whwspe	187	2	,	,	,
cord-326027-58whwspe	187	3	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	187	4	could	could	MD
cord-326027-58whwspe	187	5	become	become	VB
cord-326027-58whwspe	187	6	a	a	DT
cord-326027-58whwspe	187	7	very	very	RB
cord-326027-58whwspe	187	8	helpful	helpful	JJ
cord-326027-58whwspe	187	9	tool	tool	NN
cord-326027-58whwspe	187	10	to	to	TO
cord-326027-58whwspe	187	11	analyze	analyze	VB
cord-326027-58whwspe	187	12	viral	viral	JJ
cord-326027-58whwspe	187	13	factories	factory	NNS
cord-326027-58whwspe	187	14	assembly	assembly	NN
cord-326027-58whwspe	187	15	.	.	.
cord-326027-58whwspe	188	1	The	the	DT
cord-326027-58whwspe	188	2	strategy	strategy	NN
cord-326027-58whwspe	188	3	to	to	TO
cord-326027-58whwspe	188	4	generate	generate	VB
cord-326027-58whwspe	188	5	tagged	tagged	NNP
cord-326027-58whwspe	188	6	virus	virus	NN
cord-326027-58whwspe	188	7	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	188	8	,	,	,
cord-326027-58whwspe	188	9	consisted	consist	VBD
cord-326027-58whwspe	188	10	in	in	IN
cord-326027-58whwspe	188	11	two	two	CD
cord-326027-58whwspe	188	12	sequential	sequential	JJ
cord-326027-58whwspe	188	13	steps	step	NNS
cord-326027-58whwspe	188	14	.	.	.
cord-326027-58whwspe	189	1	After	after	IN
cord-326027-58whwspe	189	2	initial	initial	JJ
cord-326027-58whwspe	189	3	insertion	insertion	NN
cord-326027-58whwspe	189	4	of	of	IN
cord-326027-58whwspe	189	5	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	189	6	coding	code	VBG
cord-326027-58whwspe	189	7	sequence	sequence	NN
cord-326027-58whwspe	189	8	under	under	IN
cord-326027-58whwspe	189	9	the	the	DT
cord-326027-58whwspe	189	10	control	control	NN
cord-326027-58whwspe	189	11	of	of	IN
cord-326027-58whwspe	189	12	p54	p54	NN
cord-326027-58whwspe	189	13	promoter	promoter	NN
cord-326027-58whwspe	189	14	,	,	,
cord-326027-58whwspe	189	15	it	-PRON-	PRP
cord-326027-58whwspe	189	16	was	be	VBD
cord-326027-58whwspe	189	17	found	find	VBN
cord-326027-58whwspe	189	18	that	that	IN
cord-326027-58whwspe	189	19	fusion	fusion	NN
cord-326027-58whwspe	189	20	of	of	IN
cord-326027-58whwspe	189	21	EGFP	EGFP	NNP
cord-326027-58whwspe	189	22	to	to	IN
cord-326027-58whwspe	189	23	the	the	DT
cord-326027-58whwspe	189	24	C	c	NN
cord-326027-58whwspe	189	25	-	-	HYPH
cord-326027-58whwspe	189	26	terminus	terminus	NN
cord-326027-58whwspe	189	27	of	of	IN
cord-326027-58whwspe	189	28	p54	p54	NN
cord-326027-58whwspe	189	29	did	do	VBD
cord-326027-58whwspe	189	30	not	not	RB
cord-326027-58whwspe	189	31	affect	affect	VB
cord-326027-58whwspe	189	32	the	the	DT
cord-326027-58whwspe	189	33	perinuclear	perinuclear	JJ
cord-326027-58whwspe	189	34	subcellular	subcellular	JJ
cord-326027-58whwspe	189	35	distribution	distribution	NN
cord-326027-58whwspe	189	36	of	of	IN
cord-326027-58whwspe	189	37	the	the	DT
cord-326027-58whwspe	189	38	viral	viral	JJ
cord-326027-58whwspe	189	39	protein	protein	NN
cord-326027-58whwspe	189	40	,	,	,
cord-326027-58whwspe	189	41	as	as	IN
cord-326027-58whwspe	189	42	it	-PRON-	PRP
cord-326027-58whwspe	189	43	was	be	VBD
cord-326027-58whwspe	189	44	directly	directly	RB
cord-326027-58whwspe	189	45	observed	observe	VBN
cord-326027-58whwspe	189	46	in	in	IN
cord-326027-58whwspe	189	47	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	189	48	infected	infected	JJ
cord-326027-58whwspe	189	49	cells	cell	NNS
cord-326027-58whwspe	189	50	.	.	.
cord-326027-58whwspe	190	1	Then	then	RB
cord-326027-58whwspe	190	2	,	,	,
cord-326027-58whwspe	190	3	we	-PRON-	PRP
cord-326027-58whwspe	190	4	interrupted	interrupt	VBD
cord-326027-58whwspe	190	5	E183L	E183L	NNP
cord-326027-58whwspe	190	6	gene	gene	NN
cord-326027-58whwspe	190	7	in	in	IN
cord-326027-58whwspe	190	8	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	190	9	genome	genome	NN
cord-326027-58whwspe	190	10	by	by	IN
cord-326027-58whwspe	190	11	insertion	insertion	NN
cord-326027-58whwspe	190	12	of	of	IN
cord-326027-58whwspe	190	13	β	β	NN
cord-326027-58whwspe	190	14	-	-	HYPH
cord-326027-58whwspe	190	15	galactosidase	galactosidase	NN
cord-326027-58whwspe	190	16	coding	code	VBG
cord-326027-58whwspe	190	17	sequence	sequence	NN
cord-326027-58whwspe	190	18	as	as	IN
cord-326027-58whwspe	190	19	second	second	JJ
cord-326027-58whwspe	190	20	selection	selection	NN
cord-326027-58whwspe	190	21	marker	marker	NN
cord-326027-58whwspe	190	22	,	,	,
cord-326027-58whwspe	190	23	to	to	TO
cord-326027-58whwspe	190	24	ensure	ensure	VB
cord-326027-58whwspe	190	25	that	that	IN
cord-326027-58whwspe	190	26	every	every	DT
cord-326027-58whwspe	190	27	p54	p54	NN
cord-326027-58whwspe	190	28	molecule	molecule	NN
cord-326027-58whwspe	190	29	that	that	WDT
cord-326027-58whwspe	190	30	accommodates	accommodate	VBZ
cord-326027-58whwspe	190	31	in	in	IN
cord-326027-58whwspe	190	32	virion	virion	NN
cord-326027-58whwspe	190	33	inner	inner	JJ
cord-326027-58whwspe	190	34	envelope	envelope	NN
cord-326027-58whwspe	190	35	would	would	MD
cord-326027-58whwspe	190	36	be	be	VB
cord-326027-58whwspe	190	37	EGFP	EGFP	NNP
cord-326027-58whwspe	190	38	tagged	tag	VBN
cord-326027-58whwspe	190	39	.	.	.
cord-326027-58whwspe	191	1	Previously	previously	RB
cord-326027-58whwspe	191	2	,	,	,
cord-326027-58whwspe	191	3	p54	p54	NN
cord-326027-58whwspe	191	4	has	have	VBZ
cord-326027-58whwspe	191	5	been	be	VBN
cord-326027-58whwspe	191	6	shown	show	VBN
cord-326027-58whwspe	191	7	to	to	TO
cord-326027-58whwspe	191	8	be	be	VB
cord-326027-58whwspe	191	9	essential	essential	JJ
cord-326027-58whwspe	191	10	for	for	IN
cord-326027-58whwspe	191	11	virus	virus	NN
cord-326027-58whwspe	191	12	viability	viability	NN
cord-326027-58whwspe	191	13	,	,	,
cord-326027-58whwspe	191	14	playing	playing	NN
cord-326027-58whwspe	191	15	and	and	CC
cord-326027-58whwspe	191	16	important	important	JJ
cord-326027-58whwspe	191	17	role	role	NN
cord-326027-58whwspe	191	18	in	in	IN
cord-326027-58whwspe	191	19	ASFV	ASFV	NNP
cord-326027-58whwspe	191	20	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	191	21	(	(	-LRB-
cord-326027-58whwspe	192	1	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	192	2	et	et	NNP
cord-326027-58whwspe	192	3	al	al	NNP
cord-326027-58whwspe	192	4	.	.	.
cord-326027-58whwspe	193	1	,	,	,
cord-326027-58whwspe	193	2	1996	1996	CD
cord-326027-58whwspe	194	1	(	(	-LRB-
cord-326027-58whwspe	194	2	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	194	3	et	et	NNP
cord-326027-58whwspe	194	4	al	al	NNP
cord-326027-58whwspe	194	5	.	.	.
cord-326027-58whwspe	195	1	,	,	,
cord-326027-58whwspe	195	2	,	,	,
cord-326027-58whwspe	195	3	2004	2004	CD
cord-326027-58whwspe	195	4	,	,	,
cord-326027-58whwspe	195	5	so	so	CC
cord-326027-58whwspe	195	6	the	the	DT
cord-326027-58whwspe	195	7	recovery	recovery	NN
cord-326027-58whwspe	195	8	of	of	IN
cord-326027-58whwspe	195	9	recombinant	recombinant	JJ
cord-326027-58whwspe	195	10	virus	virus	NN
cord-326027-58whwspe	195	11	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	195	12	after	after	IN
cord-326027-58whwspe	195	13	wild	wild	JJ
cord-326027-58whwspe	195	14	type	type	NN
cord-326027-58whwspe	195	15	p54	p54	NN
cord-326027-58whwspe	195	16	interruption	interruption	NN
cord-326027-58whwspe	195	17	indicated	indicate	VBD
cord-326027-58whwspe	195	18	that	that	IN
cord-326027-58whwspe	195	19	fusion	fusion	NN
cord-326027-58whwspe	195	20	did	do	VBD
cord-326027-58whwspe	195	21	not	not	RB
cord-326027-58whwspe	195	22	apparently	apparently	RB
cord-326027-58whwspe	195	23	affect	affect	VB
cord-326027-58whwspe	195	24	the	the	DT
cord-326027-58whwspe	195	25	normal	normal	JJ
cord-326027-58whwspe	195	26	function	function	NN
cord-326027-58whwspe	195	27	of	of	IN
cord-326027-58whwspe	195	28	p54	p54	NN
cord-326027-58whwspe	195	29	.	.	.
cord-326027-58whwspe	196	1	Furthermore	furthermore	RB
cord-326027-58whwspe	196	2	,	,	,
cord-326027-58whwspe	196	3	growth	growth	NN
cord-326027-58whwspe	196	4	curves	curve	NNS
cord-326027-58whwspe	196	5	obtained	obtain	VBN
cord-326027-58whwspe	196	6	with	with	IN
cord-326027-58whwspe	196	7	recombinant	recombinant	JJ
cord-326027-58whwspe	196	8	virus	virus	NN
cord-326027-58whwspe	196	9	indicated	indicate	VBD
cord-326027-58whwspe	196	10	identical	identical	JJ
cord-326027-58whwspe	196	11	replication	replication	NN
cord-326027-58whwspe	196	12	behavior	behavior	NN
cord-326027-58whwspe	196	13	and	and	CC
cord-326027-58whwspe	196	14	infectious	infectious	JJ
cord-326027-58whwspe	196	15	efficiency	efficiency	NN
cord-326027-58whwspe	196	16	when	when	WRB
cord-326027-58whwspe	196	17	compared	compare	VBN
cord-326027-58whwspe	196	18	to	to	IN
cord-326027-58whwspe	196	19	the	the	DT
cord-326027-58whwspe	196	20	parental	parental	JJ
cord-326027-58whwspe	196	21	strain	strain	NN
cord-326027-58whwspe	196	22	.	.	.
cord-326027-58whwspe	197	1	Furthermore	furthermore	RB
cord-326027-58whwspe	197	2	,	,	,
cord-326027-58whwspe	197	3	fusion	fusion	NN
cord-326027-58whwspe	197	4	to	to	IN
cord-326027-58whwspe	197	5	EGFP	EGFP	NNP
cord-326027-58whwspe	197	6	did	do	VBD
cord-326027-58whwspe	197	7	not	not	RB
cord-326027-58whwspe	197	8	alter	alter	VB
cord-326027-58whwspe	197	9	the	the	DT
cord-326027-58whwspe	197	10	expression	expression	NN
cord-326027-58whwspe	197	11	pattern	pattern	NN
cord-326027-58whwspe	197	12	determined	determine	VBN
cord-326027-58whwspe	197	13	by	by	IN
cord-326027-58whwspe	197	14	WB	WB	NNP
cord-326027-58whwspe	197	15	analysis	analysis	NN
cord-326027-58whwspe	197	16	for	for	IN
cord-326027-58whwspe	197	17	p54	p54	NN
cord-326027-58whwspe	197	18	in	in	IN
cord-326027-58whwspe	197	19	infection	infection	NN
cord-326027-58whwspe	197	20	as	as	IN
cord-326027-58whwspe	197	21	p54-	p54-	NNP
cord-326027-58whwspe	197	22	EGFP	EGFP	NNP
cord-326027-58whwspe	197	23	expression	expression	NN
cord-326027-58whwspe	197	24	remained	remain	VBD
cord-326027-58whwspe	197	25	under	under	IN
cord-326027-58whwspe	197	26	control	control	NN
cord-326027-58whwspe	197	27	of	of	IN
cord-326027-58whwspe	197	28	p54	p54	CD
cord-326027-58whwspe	197	29	natural	natural	JJ
cord-326027-58whwspe	197	30	promoter	promoter	NN
cord-326027-58whwspe	197	31	in	in	IN
cord-326027-58whwspe	197	32	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	197	33	.	.	.
cord-326027-58whwspe	198	1	P54-EGFP	P54-EGFP	NNP
cord-326027-58whwspe	198	2	was	be	VBD
cord-326027-58whwspe	198	3	synthesized	synthesize	VBN
cord-326027-58whwspe	198	4	and	and	CC
cord-326027-58whwspe	198	5	detected	detect	VBN
cord-326027-58whwspe	198	6	during	during	IN
cord-326027-58whwspe	198	7	late	late	JJ
cord-326027-58whwspe	198	8	viral	viral	JJ
cord-326027-58whwspe	198	9	expression	expression	NN
cord-326027-58whwspe	198	10	phase	phase	NN
cord-326027-58whwspe	198	11	,	,	,
cord-326027-58whwspe	198	12	from	from	IN
cord-326027-58whwspe	198	13	8	8	CD
cord-326027-58whwspe	198	14	hpi	hpi	NNP
cord-326027-58whwspe	198	15	,	,	,
cord-326027-58whwspe	198	16	as	as	IN
cord-326027-58whwspe	198	17	expected	expect	VBN
cord-326027-58whwspe	198	18	.	.	.
cord-326027-58whwspe	199	1	This	this	DT
cord-326027-58whwspe	199	2	observation	observation	NN
cord-326027-58whwspe	199	3	was	be	VBD
cord-326027-58whwspe	199	4	confirmed	confirm	VBN
cord-326027-58whwspe	199	5	by	by	IN
cord-326027-58whwspe	199	6	WB	WB	NNP
cord-326027-58whwspe	199	7	analysis	analysis	NN
cord-326027-58whwspe	199	8	in	in	IN
cord-326027-58whwspe	199	9	the	the	DT
cord-326027-58whwspe	199	10	presence	presence	NN
cord-326027-58whwspe	199	11	of	of	IN
cord-326027-58whwspe	199	12	inhibitor	inhibitor	NN
cord-326027-58whwspe	199	13	Ara	Ara	NNP
cord-326027-58whwspe	199	14	-	-	HYPH
cord-326027-58whwspe	199	15	C	C	NNP
cord-326027-58whwspe	199	16	,	,	,
cord-326027-58whwspe	199	17	which	which	WDT
cord-326027-58whwspe	199	18	demonstrated	demonstrate	VBD
cord-326027-58whwspe	199	19	that	that	IN
cord-326027-58whwspe	199	20	there	there	EX
cord-326027-58whwspe	199	21	was	be	VBD
cord-326027-58whwspe	199	22	not	not	RB
cord-326027-58whwspe	199	23	chimera	chimera	NN
cord-326027-58whwspe	199	24	protein	protein	NN
cord-326027-58whwspe	199	25	expression	expression	NN
cord-326027-58whwspe	199	26	when	when	WRB
cord-326027-58whwspe	199	27	viral	viral	JJ
cord-326027-58whwspe	199	28	DNA	dna	NN
cord-326027-58whwspe	199	29	replication	replication	NN
cord-326027-58whwspe	199	30	was	be	VBD
cord-326027-58whwspe	199	31	inhibited	inhibit	VBN
cord-326027-58whwspe	199	32	.	.	.
cord-326027-58whwspe	200	1	Also	also	RB
cord-326027-58whwspe	200	2	,	,	,
cord-326027-58whwspe	200	3	expression	expression	NN
cord-326027-58whwspe	200	4	levels	level	NNS
cord-326027-58whwspe	200	5	of	of	IN
cord-326027-58whwspe	200	6	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	200	7	in	in	IN
cord-326027-58whwspe	200	8	infection	infection	NN
cord-326027-58whwspe	200	9	,	,	,
cord-326027-58whwspe	200	10	determined	determine	VBN
cord-326027-58whwspe	200	11	by	by	IN
cord-326027-58whwspe	200	12	WB	WB	NNP
cord-326027-58whwspe	200	13	,	,	,
cord-326027-58whwspe	200	14	did	do	VBD
cord-326027-58whwspe	200	15	not	not	RB
cord-326027-58whwspe	200	16	apparently	apparently	RB
cord-326027-58whwspe	200	17	differ	differ	VB
cord-326027-58whwspe	200	18	from	from	IN
cord-326027-58whwspe	200	19	those	those	DT
cord-326027-58whwspe	200	20	observed	observe	VBN
cord-326027-58whwspe	200	21	for	for	IN
cord-326027-58whwspe	200	22	p54	p54	ADD
cord-326027-58whwspe	200	23	.	.	.
cord-326027-58whwspe	201	1	In	in	IN
cord-326027-58whwspe	201	2	order	order	NN
cord-326027-58whwspe	201	3	to	to	TO
cord-326027-58whwspe	201	4	track	track	VB
cord-326027-58whwspe	201	5	single	single	JJ
cord-326027-58whwspe	201	6	fluorescent	fluorescent	NN
cord-326027-58whwspe	201	7	ASFV	ASFV	NNP
cord-326027-58whwspe	201	8	particles	particle	NNS
cord-326027-58whwspe	201	9	,	,	,
cord-326027-58whwspe	201	10	it	-PRON-	PRP
cord-326027-58whwspe	201	11	is	be	VBZ
cord-326027-58whwspe	201	12	an	an	DT
cord-326027-58whwspe	201	13	essential	essential	JJ
cord-326027-58whwspe	201	14	requirement	requirement	NN
cord-326027-58whwspe	201	15	that	that	IN
cord-326027-58whwspe	201	16	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	201	17	is	be	VBZ
cord-326027-58whwspe	201	18	incorporated	incorporate	VBN
cord-326027-58whwspe	201	19	into	into	IN
cord-326027-58whwspe	201	20	viral	viral	JJ
cord-326027-58whwspe	201	21	particles	particle	NNS
cord-326027-58whwspe	201	22	.	.	.
cord-326027-58whwspe	202	1	Previously	previously	RB
cord-326027-58whwspe	202	2	,	,	,
cord-326027-58whwspe	202	3	p54	p54	NN
cord-326027-58whwspe	202	4	has	have	VBZ
cord-326027-58whwspe	202	5	been	be	VBN
cord-326027-58whwspe	202	6	successfully	successfully	RB
cord-326027-58whwspe	202	7	used	use	VBN
cord-326027-58whwspe	202	8	as	as	IN
cord-326027-58whwspe	202	9	a	a	DT
cord-326027-58whwspe	202	10	target	target	NN
cord-326027-58whwspe	202	11	to	to	TO
cord-326027-58whwspe	202	12	generate	generate	VB
cord-326027-58whwspe	202	13	ASFV	ASFV	NNP
cord-326027-58whwspe	202	14	chimeras	chimera	NNS
cord-326027-58whwspe	202	15	incorporating	incorporate	VBG
cord-326027-58whwspe	202	16	short	short	JJ
cord-326027-58whwspe	202	17	foreign	foreign	JJ
cord-326027-58whwspe	202	18	viral	viral	JJ
cord-326027-58whwspe	202	19	epitopes	epitope	NNS
cord-326027-58whwspe	202	20	such	such	JJ
cord-326027-58whwspe	202	21	as	as	IN
cord-326027-58whwspe	202	22	the	the	DT
cord-326027-58whwspe	202	23	antigenic	antigenic	JJ
cord-326027-58whwspe	202	24	site	site	NN
cord-326027-58whwspe	202	25	A	a	NN
cord-326027-58whwspe	202	26	from	from	IN
cord-326027-58whwspe	202	27	foot	foot	NN
cord-326027-58whwspe	202	28	-	-	HYPH
cord-326027-58whwspe	202	29	and	and	CC
cord-326027-58whwspe	202	30	-	-	HYPH
cord-326027-58whwspe	202	31	mouth	mouth	NN
cord-326027-58whwspe	202	32	disease	disease	NN
cord-326027-58whwspe	202	33	virus	virus	NN
cord-326027-58whwspe	202	34	VP1	VP1	NNP
cord-326027-58whwspe	202	35	protein	protein	NN
cord-326027-58whwspe	202	36	and	and	CC
cord-326027-58whwspe	202	37	the	the	DT
cord-326027-58whwspe	202	38	DA3	DA3	NNP
cord-326027-58whwspe	202	39	antigenic	antigenic	JJ
cord-326027-58whwspe	202	40	determinant	determinant	NN
cord-326027-58whwspe	202	41	from	from	IN
cord-326027-58whwspe	202	42	transmissible	transmissible	JJ
cord-326027-58whwspe	202	43	gastroenteritis	gastroenteritis	NN
cord-326027-58whwspe	202	44	coronavirus	coronavirus	NN
cord-326027-58whwspe	203	1	nucleoprotein	nucleoprotein	NNP
cord-326027-58whwspe	203	2	N	N	NNP
cord-326027-58whwspe	203	3	(	(	-LRB-
cord-326027-58whwspe	203	4	Brun	Brun	NNP
cord-326027-58whwspe	203	5	et	et	NNP
cord-326027-58whwspe	203	6	al	al	NNP
cord-326027-58whwspe	203	7	.	.	NNP
cord-326027-58whwspe	203	8	,	,	,
cord-326027-58whwspe	203	9	1999	1999	CD
cord-326027-58whwspe	203	10	)	)	-RRB-
cord-326027-58whwspe	203	11	.	.	.
cord-326027-58whwspe	204	1	In	in	IN
cord-326027-58whwspe	204	2	both	both	DT
cord-326027-58whwspe	204	3	cases	case	NNS
cord-326027-58whwspe	204	4	,	,	,
cord-326027-58whwspe	204	5	chimeric	chimeric	JJ
cord-326027-58whwspe	204	6	p54	p54	NN
cord-326027-58whwspe	204	7	proteins	protein	NNS
cord-326027-58whwspe	204	8	were	be	VBD
cord-326027-58whwspe	204	9	successfully	successfully	RB
cord-326027-58whwspe	204	10	incorporated	incorporate	VBN
cord-326027-58whwspe	204	11	into	into	IN
cord-326027-58whwspe	204	12	the	the	DT
cord-326027-58whwspe	204	13	viral	viral	JJ
cord-326027-58whwspe	204	14	particles	particle	NNS
cord-326027-58whwspe	204	15	,	,	,
cord-326027-58whwspe	204	16	supporting	support	VBG
cord-326027-58whwspe	204	17	selection	selection	NN
cord-326027-58whwspe	204	18	of	of	IN
cord-326027-58whwspe	204	19	p54	p54	NN
cord-326027-58whwspe	204	20	as	as	IN
cord-326027-58whwspe	204	21	the	the	DT
cord-326027-58whwspe	204	22	fusion	fusion	NN
cord-326027-58whwspe	204	23	protein	protein	NN
cord-326027-58whwspe	204	24	.	.	.
cord-326027-58whwspe	205	1	As	as	IN
cord-326027-58whwspe	205	2	expected	expect	VBN
cord-326027-58whwspe	205	3	,	,	,
cord-326027-58whwspe	205	4	Western	western	JJ
cord-326027-58whwspe	205	5	blot	blot	NN
cord-326027-58whwspe	205	6	with	with	IN
cord-326027-58whwspe	205	7	Percoll	Percoll	NNP
cord-326027-58whwspe	205	8	purified	purify	VBN
cord-326027-58whwspe	205	9	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	205	10	particles	particle	NNS
cord-326027-58whwspe	205	11	demonstrated	demonstrate	VBD
cord-326027-58whwspe	205	12	the	the	DT
cord-326027-58whwspe	205	13	association	association	NN
cord-326027-58whwspe	205	14	of	of	IN
cord-326027-58whwspe	205	15	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	205	16	with	with	IN
cord-326027-58whwspe	205	17	ASFV	ASFV	NNP
cord-326027-58whwspe	205	18	virions	virion	NNS
cord-326027-58whwspe	205	19	.	.	.
cord-326027-58whwspe	206	1	As	as	IN
cord-326027-58whwspe	206	2	deduced	deduce	VBN
cord-326027-58whwspe	206	3	from	from	IN
cord-326027-58whwspe	206	4	detection	detection	NN
cord-326027-58whwspe	206	5	of	of	IN
cord-326027-58whwspe	206	6	control	control	NN
cord-326027-58whwspe	206	7	protein	protein	NN
cord-326027-58whwspe	206	8	p72	p72	NN
cord-326027-58whwspe	206	9	and	and	CC
cord-326027-58whwspe	206	10	p54	p54	NN
cord-326027-58whwspe	206	11	in	in	IN
cord-326027-58whwspe	206	12	Percoll	Percoll	NNP
cord-326027-58whwspe	206	13	purified	purify	VBN
cord-326027-58whwspe	206	14	samples	sample	NNS
cord-326027-58whwspe	206	15	,	,	,
cord-326027-58whwspe	206	16	inclusion	inclusion	NN
cord-326027-58whwspe	206	17	of	of	IN
cord-326027-58whwspe	206	18	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	206	19	into	into	IN
cord-326027-58whwspe	206	20	virions	virion	NNS
cord-326027-58whwspe	206	21	appeared	appear	VBD
cord-326027-58whwspe	206	22	to	to	TO
cord-326027-58whwspe	206	23	occur	occur	VB
cord-326027-58whwspe	206	24	with	with	IN
cord-326027-58whwspe	206	25	same	same	JJ
cord-326027-58whwspe	206	26	effectiveness	effectiveness	NN
cord-326027-58whwspe	206	27	than	than	IN
cord-326027-58whwspe	206	28	p54	p54	ADD
cord-326027-58whwspe	206	29	.	.	.
cord-326027-58whwspe	207	1	Such	such	JJ
cord-326027-58whwspe	207	2	tagged	tag	VBN
cord-326027-58whwspe	207	3	virus	virus	NN
cord-326027-58whwspe	207	4	will	will	MD
cord-326027-58whwspe	207	5	enable	enable	VB
cord-326027-58whwspe	207	6	live	live	JJ
cord-326027-58whwspe	207	7	analysis	analysis	NN
cord-326027-58whwspe	207	8	of	of	IN
cord-326027-58whwspe	207	9	various	various	JJ
cord-326027-58whwspe	207	10	stages	stage	NNS
cord-326027-58whwspe	207	11	in	in	IN
cord-326027-58whwspe	207	12	the	the	DT
cord-326027-58whwspe	207	13	ASFV	ASFV	NNP
cord-326027-58whwspe	207	14	replicative	replicative	JJ
cord-326027-58whwspe	207	15	cycle	cycle	NN
cord-326027-58whwspe	207	16	using	use	VBG
cord-326027-58whwspe	207	17	confocal	confocal	JJ
cord-326027-58whwspe	207	18	microscopy	microscopy	NNS
cord-326027-58whwspe	207	19	.	.	.
cord-326027-58whwspe	208	1	One	one	CD
cord-326027-58whwspe	208	2	of	of	IN
cord-326027-58whwspe	208	3	these	these	DT
cord-326027-58whwspe	208	4	potential	potential	JJ
cord-326027-58whwspe	208	5	applications	application	NNS
cord-326027-58whwspe	208	6	of	of	IN
cord-326027-58whwspe	208	7	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	208	8	,	,	,
cord-326027-58whwspe	208	9	shown	show	VBN
cord-326027-58whwspe	208	10	in	in	IN
cord-326027-58whwspe	208	11	present	present	JJ
cord-326027-58whwspe	208	12	work	work	NN
cord-326027-58whwspe	208	13	,	,	,
cord-326027-58whwspe	208	14	would	would	MD
cord-326027-58whwspe	208	15	be	be	VB
cord-326027-58whwspe	208	16	the	the	DT
cord-326027-58whwspe	208	17	analysis	analysis	NN
cord-326027-58whwspe	208	18	of	of	IN
cord-326027-58whwspe	208	19	intracellular	intracellular	JJ
cord-326027-58whwspe	208	20	movements	movement	NNS
cord-326027-58whwspe	208	21	of	of	IN
cord-326027-58whwspe	208	22	fluorescent	fluorescent	NN
cord-326027-58whwspe	208	23	particles	particle	NNS
cord-326027-58whwspe	208	24	during	during	IN
cord-326027-58whwspe	208	25	the	the	DT
cord-326027-58whwspe	208	26	initial	initial	JJ
cord-326027-58whwspe	208	27	phase	phase	NN
cord-326027-58whwspe	208	28	of	of	IN
cord-326027-58whwspe	208	29	infection	infection	NN
cord-326027-58whwspe	208	30	immediately	immediately	RB
cord-326027-58whwspe	208	31	after	after	IN
cord-326027-58whwspe	208	32	internalization	internalization	NN
cord-326027-58whwspe	208	33	into	into	IN
cord-326027-58whwspe	208	34	the	the	DT
cord-326027-58whwspe	208	35	host	host	NN
cord-326027-58whwspe	208	36	cell	cell	NN
cord-326027-58whwspe	208	37	.	.	.
cord-326027-58whwspe	209	1	When	when	WRB
cord-326027-58whwspe	209	2	collecting	collect	VBG
cord-326027-58whwspe	209	3	confocal	confocal	JJ
cord-326027-58whwspe	209	4	microscopic	microscopic	JJ
cord-326027-58whwspe	209	5	images	image	NNS
cord-326027-58whwspe	209	6	every	every	DT
cord-326027-58whwspe	209	7	3	3	CD
cord-326027-58whwspe	209	8	s	s	NN
cord-326027-58whwspe	209	9	,	,	,
cord-326027-58whwspe	209	10	from	from	IN
cord-326027-58whwspe	209	11	2	2	CD
cord-326027-58whwspe	209	12	hpi	hpi	NNP
cord-326027-58whwspe	209	13	,	,	,
cord-326027-58whwspe	209	14	it	-PRON-	PRP
cord-326027-58whwspe	209	15	was	be	VBD
cord-326027-58whwspe	209	16	found	find	VBN
cord-326027-58whwspe	209	17	that	that	IN
cord-326027-58whwspe	209	18	most	most	JJS
cord-326027-58whwspe	209	19	of	of	IN
cord-326027-58whwspe	209	20	the	the	DT
cord-326027-58whwspe	209	21	viral	viral	JJ
cord-326027-58whwspe	209	22	particles	particle	NNS
cord-326027-58whwspe	209	23	exhibited	exhibit	VBD
cord-326027-58whwspe	209	24	a	a	DT
cord-326027-58whwspe	209	25	special	special	JJ
cord-326027-58whwspe	209	26	kind	kind	NN
cord-326027-58whwspe	209	27	of	of	IN
cord-326027-58whwspe	209	28	movement	movement	NN
cord-326027-58whwspe	209	29	defined	define	VBN
cord-326027-58whwspe	209	30	as	as	IN
cord-326027-58whwspe	209	31	saltatory	saltatory	NN
cord-326027-58whwspe	209	32	,	,	,
cord-326027-58whwspe	209	33	intermittent	intermittent	JJ
cord-326027-58whwspe	209	34	and	and	CC
cord-326027-58whwspe	209	35	apparently	apparently	RB
cord-326027-58whwspe	209	36	disorganized	disorganized	JJ
cord-326027-58whwspe	209	37	,	,	,
cord-326027-58whwspe	209	38	making	make	VBG
cord-326027-58whwspe	209	39	difficult	difficult	JJ
cord-326027-58whwspe	209	40	the	the	DT
cord-326027-58whwspe	209	41	estimation	estimation	NN
cord-326027-58whwspe	209	42	of	of	IN
cord-326027-58whwspe	209	43	Fig	Fig	NNP
cord-326027-58whwspe	209	44	.	.	.
cord-326027-58whwspe	210	1	7	7	CD
cord-326027-58whwspe	210	2	.	.	.
cord-326027-58whwspe	211	1	Live	live	JJ
cord-326027-58whwspe	211	2	-	-	HYPH
cord-326027-58whwspe	211	3	cell	cell	NN
cord-326027-58whwspe	211	4	analysis	analysis	NN
cord-326027-58whwspe	211	5	of	of	IN
cord-326027-58whwspe	211	6	the	the	DT
cord-326027-58whwspe	211	7	last	last	JJ
cord-326027-58whwspe	211	8	stages	stage	NNS
cord-326027-58whwspe	211	9	of	of	IN
cord-326027-58whwspe	211	10	B54GFP-2	b54gfp-2	JJ
cord-326027-58whwspe	211	11	infection	infection	NN
cord-326027-58whwspe	211	12	.	.	.
cord-326027-58whwspe	212	1	Series	Series	NNP
cord-326027-58whwspe	212	2	of	of	IN
cord-326027-58whwspe	212	3	EGFP	EGFP	NNP
cord-326027-58whwspe	212	4	and	and	CC
cord-326027-58whwspe	212	5	transmitted	transmit	VBD
cord-326027-58whwspe	212	6	light	light	JJ
cord-326027-58whwspe	212	7	simultaneous	simultaneous	JJ
cord-326027-58whwspe	212	8	acquisitions	acquisition	NNS
cord-326027-58whwspe	212	9	of	of	IN
cord-326027-58whwspe	212	10	the	the	DT
cord-326027-58whwspe	212	11	same	same	JJ
cord-326027-58whwspe	212	12	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	212	13	infected	infected	JJ
cord-326027-58whwspe	212	14	Vero	Vero	NNP
cord-326027-58whwspe	212	15	cell	cell	NN
cord-326027-58whwspe	212	16	by	by	IN
cord-326027-58whwspe	212	17	confocal	confocal	JJ
cord-326027-58whwspe	212	18	microscopy	microscopy	NNS
cord-326027-58whwspe	212	19	from	from	IN
cord-326027-58whwspe	212	20	10	10	CD
cord-326027-58whwspe	212	21	hpi	hpi	NNP
cord-326027-58whwspe	212	22	(	(	-LRB-
cord-326027-58whwspe	212	23	when	when	WRB
cord-326027-58whwspe	212	24	viral	viral	JJ
cord-326027-58whwspe	212	25	factory	factory	NN
cord-326027-58whwspe	212	26	is	be	VBZ
cord-326027-58whwspe	212	27	already	already	RB
cord-326027-58whwspe	212	28	constituted	constitute	VBN
cord-326027-58whwspe	212	29	)	)	-RRB-
cord-326027-58whwspe	212	30	and	and	CC
cord-326027-58whwspe	212	31	every	every	DT
cord-326027-58whwspe	212	32	5	5	CD
cord-326027-58whwspe	212	33	min	min	NN
cord-326027-58whwspe	212	34	(	(	-LRB-
cord-326027-58whwspe	212	35	see	see	VB
cord-326027-58whwspe	212	36	additional	additional	JJ
cord-326027-58whwspe	212	37	file	file	NN
cord-326027-58whwspe	212	38	3	3	CD
cord-326027-58whwspe	212	39	to	to	TO
cord-326027-58whwspe	212	40	complete	complete	VB
cord-326027-58whwspe	212	41	animation	animation	NN
cord-326027-58whwspe	212	42	sequence	sequence	NN
cord-326027-58whwspe	212	43	)	)	-RRB-
cord-326027-58whwspe	212	44	.	.	.
cord-326027-58whwspe	213	1	Rounding	round	VBG
cord-326027-58whwspe	213	2	of	of	IN
cord-326027-58whwspe	213	3	the	the	DT
cord-326027-58whwspe	213	4	infected	infected	JJ
cord-326027-58whwspe	213	5	cell	cell	NN
cord-326027-58whwspe	213	6	is	be	VBZ
cord-326027-58whwspe	213	7	followed	follow	VBN
cord-326027-58whwspe	213	8	by	by	IN
cord-326027-58whwspe	213	9	membrane	membrane	NN
cord-326027-58whwspe	213	10	blebbing	blebbing	NN
cord-326027-58whwspe	213	11	and	and	CC
cord-326027-58whwspe	213	12	subsequent	subsequent	JJ
cord-326027-58whwspe	213	13	formation	formation	NN
cord-326027-58whwspe	213	14	of	of	IN
cord-326027-58whwspe	213	15	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	213	16	(	(	-LRB-
cord-326027-58whwspe	213	17	green	green	JJ
cord-326027-58whwspe	213	18	)	)	-RRB-
cord-326027-58whwspe	213	19	containing	contain	VBG
cord-326027-58whwspe	213	20	vesicles	vesicle	NNS
cord-326027-58whwspe	213	21	.	.	.
cord-326027-58whwspe	214	1	At	at	IN
cord-326027-58whwspe	214	2	18	18	CD
cord-326027-58whwspe	214	3	hpi	hpi	NNP
cord-326027-58whwspe	214	4	,	,	,
cord-326027-58whwspe	214	5	detachment	detachment	NN
cord-326027-58whwspe	214	6	and	and	CC
cord-326027-58whwspe	214	7	final	final	JJ
cord-326027-58whwspe	214	8	cell	cell	NN
cord-326027-58whwspe	214	9	destruction	destruction	NN
cord-326027-58whwspe	214	10	is	be	VBZ
cord-326027-58whwspe	214	11	observed	observe	VBN
cord-326027-58whwspe	214	12	.	.	.
cord-326027-58whwspe	215	1	Scale	scale	NN
cord-326027-58whwspe	215	2	bar	bar	NN
cord-326027-58whwspe	215	3	,	,	,
cord-326027-58whwspe	215	4	20	20	CD
cord-326027-58whwspe	215	5	μm	μm	NN
cord-326027-58whwspe	215	6	.	.	.
cord-326027-58whwspe	216	1	(	(	-LRB-
cord-326027-58whwspe	216	2	For	for	IN
cord-326027-58whwspe	216	3	interpretation	interpretation	NN
cord-326027-58whwspe	216	4	of	of	IN
cord-326027-58whwspe	216	5	the	the	DT
cord-326027-58whwspe	216	6	references	reference	NNS
cord-326027-58whwspe	216	7	to	to	TO
cord-326027-58whwspe	216	8	colour	colour	VB
cord-326027-58whwspe	216	9	in	in	IN
cord-326027-58whwspe	216	10	this	this	DT
cord-326027-58whwspe	216	11	figure	figure	NN
cord-326027-58whwspe	216	12	legend	legend	NN
cord-326027-58whwspe	216	13	,	,	,
cord-326027-58whwspe	216	14	the	the	DT
cord-326027-58whwspe	216	15	reader	reader	NN
cord-326027-58whwspe	216	16	is	be	VBZ
cord-326027-58whwspe	216	17	referred	refer	VBN
cord-326027-58whwspe	216	18	to	to	IN
cord-326027-58whwspe	216	19	the	the	DT
cord-326027-58whwspe	216	20	web	web	NN
cord-326027-58whwspe	216	21	version	version	NN
cord-326027-58whwspe	216	22	of	of	IN
cord-326027-58whwspe	216	23	this	this	DT
cord-326027-58whwspe	216	24	article	article	NN
cord-326027-58whwspe	216	25	.	.	.
cord-326027-58whwspe	216	26	)	)	-RRB-
cord-326027-58whwspe	217	1	speed	speed	NN
cord-326027-58whwspe	217	2	rates	rate	NNS
cord-326027-58whwspe	217	3	.	.	.
cord-326027-58whwspe	218	1	This	this	DT
cord-326027-58whwspe	218	2	type	type	NN
cord-326027-58whwspe	218	3	of	of	IN
cord-326027-58whwspe	218	4	movement	movement	NN
cord-326027-58whwspe	218	5	has	have	VBZ
cord-326027-58whwspe	218	6	been	be	VBN
cord-326027-58whwspe	218	7	described	describe	VBN
cord-326027-58whwspe	218	8	as	as	IN
cord-326027-58whwspe	218	9	characteristic	characteristic	NN
cord-326027-58whwspe	218	10	of	of	IN
cord-326027-58whwspe	218	11	microtubule	microtubule	JJ
cord-326027-58whwspe	218	12	associated	associate	VBN
cord-326027-58whwspe	218	13	movement	movement	NN
cord-326027-58whwspe	218	14	.	.	.
cord-326027-58whwspe	219	1	From	from	IN
cord-326027-58whwspe	219	2	the	the	DT
cord-326027-58whwspe	219	3	analysis	analysis	NN
cord-326027-58whwspe	219	4	of	of	IN
cord-326027-58whwspe	219	5	movement	movement	NN
cord-326027-58whwspe	219	6	of	of	IN
cord-326027-58whwspe	219	7	10	10	CD
cord-326027-58whwspe	219	8	individual	individual	JJ
cord-326027-58whwspe	219	9	virions	virion	NNS
cord-326027-58whwspe	219	10	an	an	DT
cord-326027-58whwspe	219	11	average	average	JJ
cord-326027-58whwspe	219	12	speed	speed	NN
cord-326027-58whwspe	219	13	ranging	range	VBG
cord-326027-58whwspe	219	14	from	from	IN
cord-326027-58whwspe	219	15	0.2	0.2	CD
cord-326027-58whwspe	219	16	to	to	IN
cord-326027-58whwspe	219	17	0.5	0.5	CD
cord-326027-58whwspe	219	18	μm	μm	NN
cord-326027-58whwspe	219	19	/	/	SYM
cord-326027-58whwspe	219	20	s	s	POS
cord-326027-58whwspe	219	21	was	be	VBD
cord-326027-58whwspe	219	22	calculated	calculate	VBN
cord-326027-58whwspe	219	23	with	with	IN
cord-326027-58whwspe	219	24	the	the	DT
cord-326027-58whwspe	219	25	applied	apply	VBN
cord-326027-58whwspe	219	26	resolution	resolution	NN
cord-326027-58whwspe	219	27	.	.	.
cord-326027-58whwspe	220	1	These	these	DT
cord-326027-58whwspe	220	2	data	datum	NNS
cord-326027-58whwspe	220	3	are	be	VBP
cord-326027-58whwspe	220	4	in	in	IN
cord-326027-58whwspe	220	5	agreement	agreement	NN
cord-326027-58whwspe	220	6	with	with	IN
cord-326027-58whwspe	220	7	those	those	DT
cord-326027-58whwspe	220	8	obtained	obtain	VBN
cord-326027-58whwspe	220	9	for	for	IN
cord-326027-58whwspe	220	10	other	other	JJ
cord-326027-58whwspe	220	11	large	large	JJ
cord-326027-58whwspe	220	12	enveloped	envelop	VBN
cord-326027-58whwspe	220	13	viruses	virus	NNS
cord-326027-58whwspe	220	14	.	.	.
cord-326027-58whwspe	221	1	Using	use	VBG
cord-326027-58whwspe	221	2	fluorescence	fluorescence	NN
cord-326027-58whwspe	221	3	videomicroscopy	videomicroscopy	NN
cord-326027-58whwspe	221	4	,	,	,
cord-326027-58whwspe	221	5	which	which	WDT
cord-326027-58whwspe	221	6	allows	allow	VBZ
cord-326027-58whwspe	221	7	a	a	DT
cord-326027-58whwspe	221	8	resolution	resolution	NN
cord-326027-58whwspe	221	9	of	of	IN
cord-326027-58whwspe	221	10	1	1	CD
cord-326027-58whwspe	221	11	frame	frame	NN
cord-326027-58whwspe	221	12	/	/	SYM
cord-326027-58whwspe	221	13	s	s	NN
cord-326027-58whwspe	221	14	,	,	,
cord-326027-58whwspe	221	15	speed	speed	NN
cord-326027-58whwspe	221	16	rates	rate	NNS
cord-326027-58whwspe	221	17	from	from	IN
cord-326027-58whwspe	221	18	0.3	0.3	CD
cord-326027-58whwspe	221	19	to	to	IN
cord-326027-58whwspe	221	20	1	1	CD
cord-326027-58whwspe	221	21	μm	μm	NN
cord-326027-58whwspe	221	22	/	/	SYM
cord-326027-58whwspe	221	23	s	s	PRP
cord-326027-58whwspe	221	24	have	have	VBP
cord-326027-58whwspe	221	25	been	be	VBN
cord-326027-58whwspe	221	26	determined	determine	VBN
cord-326027-58whwspe	221	27	during	during	IN
cord-326027-58whwspe	221	28	the	the	DT
cord-326027-58whwspe	221	29	late	late	JJ
cord-326027-58whwspe	221	30	phase	phase	NN
cord-326027-58whwspe	221	31	of	of	IN
cord-326027-58whwspe	221	32	infection	infection	NN
cord-326027-58whwspe	221	33	with	with	IN
cord-326027-58whwspe	221	34	labeled	label	VBN
cord-326027-58whwspe	221	35	vaccinia	vaccinia	NN
cord-326027-58whwspe	221	36	virus	virus	NN
cord-326027-58whwspe	221	37	(	(	-LRB-
cord-326027-58whwspe	221	38	Geada	Geada	NNP
cord-326027-58whwspe	221	39	et	et	FW
cord-326027-58whwspe	221	40	al	al	NNP
cord-326027-58whwspe	221	41	.	.	NNP
cord-326027-58whwspe	221	42	,	,	,
cord-326027-58whwspe	221	43	2001	2001	CD
cord-326027-58whwspe	221	44	;	;	:
cord-326027-58whwspe	221	45	Ward	Ward	NNP
cord-326027-58whwspe	221	46	,	,	,
cord-326027-58whwspe	221	47	2005	2005	CD
cord-326027-58whwspe	221	48	;	;	:
cord-326027-58whwspe	221	49	Ward	Ward	NNP
cord-326027-58whwspe	221	50	and	and	CC
cord-326027-58whwspe	221	51	Moss	Moss	NNP
cord-326027-58whwspe	221	52	,	,	,
cord-326027-58whwspe	221	53	2001	2001	CD
cord-326027-58whwspe	221	54	)	)	-RRB-
cord-326027-58whwspe	221	55	.	.	.
cord-326027-58whwspe	222	1	Recently	recently	RB
cord-326027-58whwspe	222	2	,	,	,
cord-326027-58whwspe	222	3	human	human	JJ
cord-326027-58whwspe	222	4	cytomegalovirus	cytomegalovirus	NN
cord-326027-58whwspe	222	5	has	have	VBZ
cord-326027-58whwspe	222	6	been	be	VBN
cord-326027-58whwspe	222	7	shown	show	VBN
cord-326027-58whwspe	222	8	to	to	TO
cord-326027-58whwspe	222	9	move	move	VB
cord-326027-58whwspe	222	10	with	with	IN
cord-326027-58whwspe	222	11	apparent	apparent	JJ
cord-326027-58whwspe	222	12	velocity	velocity	NN
cord-326027-58whwspe	222	13	of	of	IN
cord-326027-58whwspe	222	14	0.7	0.7	CD
cord-326027-58whwspe	222	15	to	to	IN
cord-326027-58whwspe	222	16	0.8	0.8	CD
cord-326027-58whwspe	223	1	μm/	μm/	NNP
cord-326027-58whwspe	223	2	s	s	POS
cord-326027-58whwspe	224	1	(	(	-LRB-
cord-326027-58whwspe	224	2	Sampaio	Sampaio	NNP
cord-326027-58whwspe	224	3	et	et	NNP
cord-326027-58whwspe	224	4	al	al	NNP
cord-326027-58whwspe	224	5	.	.	NNP
cord-326027-58whwspe	224	6	,	,	,
cord-326027-58whwspe	224	7	2005	2005	CD
cord-326027-58whwspe	224	8	)	)	-RRB-
cord-326027-58whwspe	225	1	at	at	IN
cord-326027-58whwspe	225	2	very	very	RB
cord-326027-58whwspe	225	3	early	early	JJ
cord-326027-58whwspe	225	4	stages	stage	NNS
cord-326027-58whwspe	225	5	in	in	IN
cord-326027-58whwspe	225	6	infection	infection	NN
cord-326027-58whwspe	225	7	.	.	.
cord-326027-58whwspe	226	1	Moreover	moreover	RB
cord-326027-58whwspe	226	2	,	,	,
cord-326027-58whwspe	226	3	HIV	HIV	NNP
cord-326027-58whwspe	226	4	virions	virion	NNS
cord-326027-58whwspe	226	5	transport	transport	NN
cord-326027-58whwspe	226	6	towards	towards	IN
cord-326027-58whwspe	226	7	host	host	NN
cord-326027-58whwspe	226	8	cell	cell	NN
cord-326027-58whwspe	226	9	nucleus	nucleus	NN
cord-326027-58whwspe	226	10	linked	link	VBN
cord-326027-58whwspe	226	11	to	to	IN
cord-326027-58whwspe	226	12	microtubules	microtubule	NNS
cord-326027-58whwspe	226	13	occurs	occur	VBZ
cord-326027-58whwspe	226	14	at	at	IN
cord-326027-58whwspe	226	15	1	1	CD
cord-326027-58whwspe	226	16	μm	μm	NN
cord-326027-58whwspe	226	17	/	/	SYM
cord-326027-58whwspe	226	18	s	s	NN
cord-326027-58whwspe	227	1	(	(	-LRB-
cord-326027-58whwspe	227	2	McDonald	McDonald	NNP
cord-326027-58whwspe	227	3	et	et	NNP
cord-326027-58whwspe	227	4	al	al	NNP
cord-326027-58whwspe	227	5	.	.	NNP
cord-326027-58whwspe	227	6	,	,	,
cord-326027-58whwspe	227	7	2002	2002	CD
cord-326027-58whwspe	227	8	)	)	-RRB-
cord-326027-58whwspe	227	9	.	.	.
cord-326027-58whwspe	228	1	The	the	DT
cord-326027-58whwspe	228	2	saltatory	saltatory	NN
cord-326027-58whwspe	228	3	movements	movement	NNS
cord-326027-58whwspe	228	4	of	of	IN
cord-326027-58whwspe	228	5	tagged	tagged	NNP
cord-326027-58whwspe	228	6	ASFV	ASFV	NNP
cord-326027-58whwspe	228	7	virions	virion	NNS
cord-326027-58whwspe	228	8	and	and	CC
cord-326027-58whwspe	228	9	the	the	DT
cord-326027-58whwspe	228	10	speed	speed	NN
cord-326027-58whwspe	228	11	rates	rate	NNS
cord-326027-58whwspe	228	12	obtained	obtain	VBN
cord-326027-58whwspe	228	13	are	be	VBP
cord-326027-58whwspe	228	14	consistent	consistent	JJ
cord-326027-58whwspe	228	15	with	with	IN
cord-326027-58whwspe	228	16	a	a	DT
cord-326027-58whwspe	228	17	microtubular	microtubular	NN
cord-326027-58whwspe	228	18	based	base	VBN
cord-326027-58whwspe	228	19	transport	transport	NN
cord-326027-58whwspe	228	20	(	(	-LRB-
cord-326027-58whwspe	228	21	King	King	NNP
cord-326027-58whwspe	228	22	,	,	,
cord-326027-58whwspe	228	23	2000	2000	CD
cord-326027-58whwspe	228	24	)	)	-RRB-
cord-326027-58whwspe	228	25	and	and	CC
cord-326027-58whwspe	228	26	association	association	NN
cord-326027-58whwspe	228	27	of	of	IN
cord-326027-58whwspe	228	28	this	this	DT
cord-326027-58whwspe	228	29	virus	virus	NN
cord-326027-58whwspe	228	30	with	with	IN
cord-326027-58whwspe	228	31	microtubules	microtubule	NNS
cord-326027-58whwspe	228	32	has	have	VBZ
cord-326027-58whwspe	228	33	been	be	VBN
cord-326027-58whwspe	228	34	described	describe	VBN
cord-326027-58whwspe	228	35	(	(	-LRB-
cord-326027-58whwspe	228	36	de	de	FW
cord-326027-58whwspe	228	37	Matos	Matos	NNP
cord-326027-58whwspe	228	38	and	and	CC
cord-326027-58whwspe	228	39	Carvalho	Carvalho	NNP
cord-326027-58whwspe	228	40	,	,	,
cord-326027-58whwspe	228	41	1993	1993	CD
cord-326027-58whwspe	228	42	)	)	-RRB-
cord-326027-58whwspe	228	43	.	.	.
cord-326027-58whwspe	229	1	Previously	previously	RB
cord-326027-58whwspe	229	2	,	,	,
cord-326027-58whwspe	229	3	we	-PRON-	PRP
cord-326027-58whwspe	229	4	identified	identify	VBD
cord-326027-58whwspe	229	5	the	the	DT
cord-326027-58whwspe	229	6	LC8	lc8	JJ
cord-326027-58whwspe	229	7	subunit	subunit	NN
cord-326027-58whwspe	229	8	of	of	IN
cord-326027-58whwspe	229	9	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	229	10	dynein	dynein	NNP
cord-326027-58whwspe	229	11	,	,	,
cord-326027-58whwspe	229	12	a	a	DT
cord-326027-58whwspe	229	13	minus	minus	JJ
cord-326027-58whwspe	229	14	end	end	NN
cord-326027-58whwspe	229	15	directed	direct	VBN
cord-326027-58whwspe	229	16	microtubule	microtubule	NN
cord-326027-58whwspe	229	17	-	-	HYPH
cord-326027-58whwspe	229	18	associated	associate	VBN
cord-326027-58whwspe	229	19	motor	motor	NN
cord-326027-58whwspe	229	20	protein	protein	NN
cord-326027-58whwspe	229	21	,	,	,
cord-326027-58whwspe	229	22	as	as	IN
cord-326027-58whwspe	229	23	an	an	DT
cord-326027-58whwspe	229	24	interacting	interact	VBG
cord-326027-58whwspe	229	25	protein	protein	NN
cord-326027-58whwspe	229	26	with	with	IN
cord-326027-58whwspe	229	27	p54	p54	NN
cord-326027-58whwspe	229	28	(	(	-LRB-
cord-326027-58whwspe	229	29	Alonso	Alonso	NNP
cord-326027-58whwspe	229	30	et	et	NNP
cord-326027-58whwspe	229	31	al	al	NNP
cord-326027-58whwspe	229	32	.	.	NNP
cord-326027-58whwspe	229	33	,	,	,
cord-326027-58whwspe	229	34	2001	2001	CD
cord-326027-58whwspe	229	35	)	)	-RRB-
cord-326027-58whwspe	229	36	,	,	,
cord-326027-58whwspe	229	37	resulting	result	VBG
cord-326027-58whwspe	229	38	this	this	DT
cord-326027-58whwspe	229	39	interaction	interaction	NN
cord-326027-58whwspe	229	40	critical	critical	JJ
cord-326027-58whwspe	229	41	in	in	IN
cord-326027-58whwspe	229	42	the	the	DT
cord-326027-58whwspe	229	43	first	first	JJ
cord-326027-58whwspe	229	44	stages	stage	NNS
cord-326027-58whwspe	229	45	of	of	IN
cord-326027-58whwspe	229	46	ASFV	ASFV	NNP
cord-326027-58whwspe	229	47	infection	infection	NN
cord-326027-58whwspe	229	48	.	.	.
cord-326027-58whwspe	230	1	Collectively	collectively	RB
cord-326027-58whwspe	230	2	,	,	,
cord-326027-58whwspe	230	3	these	these	DT
cord-326027-58whwspe	230	4	data	datum	NNS
cord-326027-58whwspe	230	5	suggested	suggest	VBD
cord-326027-58whwspe	230	6	a	a	DT
cord-326027-58whwspe	230	7	role	role	NN
cord-326027-58whwspe	230	8	for	for	IN
cord-326027-58whwspe	230	9	microtubules	microtubule	NNS
cord-326027-58whwspe	230	10	in	in	IN
cord-326027-58whwspe	230	11	the	the	DT
cord-326027-58whwspe	230	12	intracellular	intracellular	JJ
cord-326027-58whwspe	230	13	transport	transport	NN
cord-326027-58whwspe	230	14	of	of	IN
cord-326027-58whwspe	230	15	ASFV	ASFV	NNP
cord-326027-58whwspe	230	16	after	after	IN
cord-326027-58whwspe	230	17	internalization	internalization	NN
cord-326027-58whwspe	230	18	.	.	.
cord-326027-58whwspe	231	1	This	this	DT
cord-326027-58whwspe	231	2	role	role	NN
cord-326027-58whwspe	231	3	is	be	VBZ
cord-326027-58whwspe	231	4	supported	support	VBN
cord-326027-58whwspe	231	5	by	by	IN
cord-326027-58whwspe	231	6	data	datum	NNS
cord-326027-58whwspe	231	7	showing	show	VBG
cord-326027-58whwspe	231	8	that	that	IN
cord-326027-58whwspe	231	9	nocodazole	nocodazole	NNP
cord-326027-58whwspe	231	10	disruption	disruption	NN
cord-326027-58whwspe	231	11	of	of	IN
cord-326027-58whwspe	231	12	microtubules	microtubule	NNS
cord-326027-58whwspe	231	13	prior	prior	RB
cord-326027-58whwspe	231	14	to	to	IN
cord-326027-58whwspe	231	15	infection	infection	NN
cord-326027-58whwspe	231	16	results	result	NNS
cord-326027-58whwspe	231	17	in	in	IN
cord-326027-58whwspe	231	18	stop	stop	NN
cord-326027-58whwspe	231	19	of	of	IN
cord-326027-58whwspe	231	20	virion	virion	NN
cord-326027-58whwspe	231	21	movement	movement	NN
cord-326027-58whwspe	231	22	and	and	CC
cord-326027-58whwspe	231	23	lack	lack	NN
cord-326027-58whwspe	231	24	of	of	IN
cord-326027-58whwspe	231	25	perinuclear	perinuclear	NNP
cord-326027-58whwspe	231	26	accumulation	accumulation	NN
cord-326027-58whwspe	231	27	of	of	IN
cord-326027-58whwspe	231	28	viral	viral	JJ
cord-326027-58whwspe	231	29	particles	particle	NNS
cord-326027-58whwspe	231	30	at	at	IN
cord-326027-58whwspe	231	31	4	4	CD
cord-326027-58whwspe	231	32	hpi	hpi	NNP
cord-326027-58whwspe	231	33	that	that	WDT
cord-326027-58whwspe	231	34	remained	remain	VBD
cord-326027-58whwspe	231	35	dispersed	disperse	VBN
cord-326027-58whwspe	231	36	in	in	IN
cord-326027-58whwspe	231	37	the	the	DT
cord-326027-58whwspe	231	38	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	231	39	.	.	.
cord-326027-58whwspe	232	1	Also	also	RB
cord-326027-58whwspe	232	2	,	,	,
cord-326027-58whwspe	232	3	there	there	EX
cord-326027-58whwspe	232	4	was	be	VBD
cord-326027-58whwspe	232	5	no	no	DT
cord-326027-58whwspe	232	6	subsequent	subsequent	JJ
cord-326027-58whwspe	232	7	constitution	constitution	NN
cord-326027-58whwspe	232	8	of	of	IN
cord-326027-58whwspe	232	9	the	the	DT
cord-326027-58whwspe	232	10	characteristic	characteristic	JJ
cord-326027-58whwspe	232	11	virus	virus	NN
cord-326027-58whwspe	232	12	factory	factory	NN
cord-326027-58whwspe	232	13	and	and	CC
cord-326027-58whwspe	232	14	finally	finally	RB
cord-326027-58whwspe	232	15	a	a	DT
cord-326027-58whwspe	232	16	reduction	reduction	NN
cord-326027-58whwspe	232	17	in	in	IN
cord-326027-58whwspe	232	18	ASFV	ASFV	NNP
cord-326027-58whwspe	232	19	progeny	progeny	NN
cord-326027-58whwspe	232	20	was	be	VBD
cord-326027-58whwspe	232	21	found	find	VBN
cord-326027-58whwspe	232	22	(	(	-LRB-
cord-326027-58whwspe	232	23	additional	additional	JJ
cord-326027-58whwspe	232	24	files	file	NNS
cord-326027-58whwspe	232	25	1	1	CD
cord-326027-58whwspe	232	26	and	and	CC
cord-326027-58whwspe	232	27	2	2	CD
cord-326027-58whwspe	232	28	and	and	CC
cord-326027-58whwspe	232	29	Alonso	Alonso	NNP
cord-326027-58whwspe	232	30	et	et	NNP
cord-326027-58whwspe	232	31	al	al	NNP
cord-326027-58whwspe	232	32	.	.	NNP
cord-326027-58whwspe	232	33	,	,	,
cord-326027-58whwspe	232	34	2001	2001	CD
cord-326027-58whwspe	232	35	)	)	-RRB-
cord-326027-58whwspe	232	36	.	.	.
cord-326027-58whwspe	233	1	Also	also	RB
cord-326027-58whwspe	233	2	,	,	,
cord-326027-58whwspe	233	3	during	during	IN
cord-326027-58whwspe	233	4	the	the	DT
cord-326027-58whwspe	233	5	late	late	JJ
cord-326027-58whwspe	233	6	phase	phase	NN
cord-326027-58whwspe	233	7	of	of	IN
cord-326027-58whwspe	233	8	ASFV	ASFV	NNP
cord-326027-58whwspe	233	9	infection	infection	NN
cord-326027-58whwspe	233	10	,	,	,
cord-326027-58whwspe	233	11	transport	transport	NN
cord-326027-58whwspe	233	12	of	of	IN
cord-326027-58whwspe	233	13	newly	newly	RB
cord-326027-58whwspe	233	14	assembled	assemble	VBN
cord-326027-58whwspe	233	15	virions	virion	NNS
cord-326027-58whwspe	233	16	from	from	IN
cord-326027-58whwspe	233	17	viral	viral	JJ
cord-326027-58whwspe	233	18	factory	factory	NN
cord-326027-58whwspe	233	19	to	to	IN
cord-326027-58whwspe	233	20	cell	cell	NN
cord-326027-58whwspe	233	21	periphery	periphery	NN
cord-326027-58whwspe	233	22	along	along	IN
cord-326027-58whwspe	233	23	microtubules	microtubule	NNS
cord-326027-58whwspe	233	24	has	have	VBZ
cord-326027-58whwspe	233	25	been	be	VBN
cord-326027-58whwspe	233	26	recently	recently	RB
cord-326027-58whwspe	233	27	demonstrated	demonstrate	VBN
cord-326027-58whwspe	233	28	(	(	-LRB-
cord-326027-58whwspe	233	29	Stefanovic	Stefanovic	NNP
cord-326027-58whwspe	233	30	et	et	FW
cord-326027-58whwspe	233	31	al	al	NNP
cord-326027-58whwspe	233	32	.	.	NNP
cord-326027-58whwspe	233	33	,	,	,
cord-326027-58whwspe	233	34	2005	2005	CD
cord-326027-58whwspe	233	35	)	)	-RRB-
cord-326027-58whwspe	233	36	,	,	,
cord-326027-58whwspe	233	37	similar	similar	JJ
cord-326027-58whwspe	233	38	to	to	IN
cord-326027-58whwspe	233	39	the	the	DT
cord-326027-58whwspe	233	40	exit	exit	NN
cord-326027-58whwspe	233	41	transport	transport	NN
cord-326027-58whwspe	233	42	described	describe	VBN
cord-326027-58whwspe	233	43	for	for	IN
cord-326027-58whwspe	233	44	vaccinia	vaccinia	NN
cord-326027-58whwspe	233	45	virus	virus	NN
cord-326027-58whwspe	233	46	(	(	-LRB-
cord-326027-58whwspe	233	47	Carter	Carter	NNP
cord-326027-58whwspe	233	48	et	et	NNP
cord-326027-58whwspe	233	49	al	al	NNP
cord-326027-58whwspe	233	50	.	.	NNP
cord-326027-58whwspe	233	51	,	,	,
cord-326027-58whwspe	233	52	2003	2003	CD
cord-326027-58whwspe	233	53	)	)	-RRB-
cord-326027-58whwspe	233	54	.	.	.
cord-326027-58whwspe	234	1	Further	further	JJ
cord-326027-58whwspe	234	2	live	live	JJ
cord-326027-58whwspe	234	3	studies	study	NNS
cord-326027-58whwspe	234	4	involving	involve	VBG
cord-326027-58whwspe	234	5	labeled	label	VBN
cord-326027-58whwspe	234	6	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	234	7	that	that	WDT
cord-326027-58whwspe	234	8	are	be	VBP
cord-326027-58whwspe	234	9	now	now	RB
cord-326027-58whwspe	234	10	in	in	IN
cord-326027-58whwspe	234	11	course	course	NN
cord-326027-58whwspe	234	12	will	will	MD
cord-326027-58whwspe	234	13	provide	provide	VB
cord-326027-58whwspe	234	14	useful	useful	JJ
cord-326027-58whwspe	234	15	and	and	CC
cord-326027-58whwspe	234	16	detailed	detailed	JJ
cord-326027-58whwspe	234	17	information	information	NN
cord-326027-58whwspe	234	18	to	to	TO
cord-326027-58whwspe	234	19	clarify	clarify	VB
cord-326027-58whwspe	234	20	the	the	DT
cord-326027-58whwspe	234	21	whole	whole	JJ
cord-326027-58whwspe	234	22	ASFV	ASFV	NNP
cord-326027-58whwspe	234	23	penetration	penetration	NN
cord-326027-58whwspe	234	24	and	and	CC
cord-326027-58whwspe	234	25	egress	egress	NN
cord-326027-58whwspe	234	26	process	process	NN
cord-326027-58whwspe	234	27	into	into	IN
cord-326027-58whwspe	234	28	the	the	DT
cord-326027-58whwspe	234	29	host	host	NN
cord-326027-58whwspe	234	30	cell	cell	NN
cord-326027-58whwspe	234	31	.	.	.
cord-326027-58whwspe	235	1	P54	P54	NNP
cord-326027-58whwspe	235	2	has	have	VBZ
cord-326027-58whwspe	235	3	been	be	VBN
cord-326027-58whwspe	235	4	previously	previously	RB
cord-326027-58whwspe	235	5	described	describe	VBN
cord-326027-58whwspe	235	6	to	to	TO
cord-326027-58whwspe	235	7	localize	localize	VB
cord-326027-58whwspe	235	8	in	in	IN
cord-326027-58whwspe	235	9	membrane	membrane	NN
cord-326027-58whwspe	235	10	structures	structure	NNS
cord-326027-58whwspe	235	11	within	within	IN
cord-326027-58whwspe	235	12	the	the	DT
cord-326027-58whwspe	235	13	virus	virus	NN
cord-326027-58whwspe	235	14	factories	factory	NNS
cord-326027-58whwspe	235	15	and	and	CC
cord-326027-58whwspe	235	16	in	in	IN
cord-326027-58whwspe	235	17	immature	immature	JJ
cord-326027-58whwspe	235	18	ASF	ASF	NNP
cord-326027-58whwspe	235	19	virions	virion	NNS
cord-326027-58whwspe	235	20	,	,	,
cord-326027-58whwspe	235	21	as	as	IN
cord-326027-58whwspe	235	22	judged	judge	VBN
cord-326027-58whwspe	235	23	by	by	IN
cord-326027-58whwspe	235	24	immunogold	immunogold	JJ
cord-326027-58whwspe	235	25	electron	electron	NN
cord-326027-58whwspe	235	26	microscopy	microscopy	NNP
cord-326027-58whwspe	235	27	(	(	-LRB-
cord-326027-58whwspe	235	28	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	235	29	et	et	NNP
cord-326027-58whwspe	235	30	al	al	NNP
cord-326027-58whwspe	235	31	.	.	NNP
cord-326027-58whwspe	235	32	,	,	,
cord-326027-58whwspe	235	33	1994	1994	CD
cord-326027-58whwspe	235	34	)	)	-RRB-
cord-326027-58whwspe	235	35	.	.	.
cord-326027-58whwspe	236	1	P54-EGFP	P54-EGFP	NNP
cord-326027-58whwspe	236	2	,	,	,
cord-326027-58whwspe	236	3	just	just	RB
cord-326027-58whwspe	236	4	as	as	IN
cord-326027-58whwspe	236	5	p54	p54	CD
cord-326027-58whwspe	236	6	,	,	,
cord-326027-58whwspe	236	7	showed	show	VBD
cord-326027-58whwspe	236	8	an	an	DT
cord-326027-58whwspe	236	9	intracellular	intracellular	JJ
cord-326027-58whwspe	236	10	distribution	distribution	NN
cord-326027-58whwspe	236	11	fully	fully	RB
cord-326027-58whwspe	236	12	coincident	coincident	JJ
cord-326027-58whwspe	236	13	with	with	IN
cord-326027-58whwspe	236	14	ASFV	ASFV	NNP
cord-326027-58whwspe	236	15	factory	factory	NN
cord-326027-58whwspe	236	16	at	at	IN
cord-326027-58whwspe	236	17	late	late	JJ
cord-326027-58whwspe	236	18	postinfection	postinfection	NN
cord-326027-58whwspe	236	19	times	time	NNS
cord-326027-58whwspe	236	20	.	.	.
cord-326027-58whwspe	237	1	This	this	DT
cord-326027-58whwspe	237	2	allowed	allow	VBD
cord-326027-58whwspe	237	3	us	-PRON-	PRP
cord-326027-58whwspe	237	4	,	,	,
cord-326027-58whwspe	237	5	to	to	TO
cord-326027-58whwspe	237	6	follow	follow	VB
cord-326027-58whwspe	237	7	the	the	DT
cord-326027-58whwspe	237	8	viral	viral	JJ
cord-326027-58whwspe	237	9	factory	factory	NN
cord-326027-58whwspe	237	10	formation	formation	NN
cord-326027-58whwspe	237	11	process	process	NN
cord-326027-58whwspe	237	12	in	in	IN
cord-326027-58whwspe	237	13	live	live	JJ
cord-326027-58whwspe	237	14	cells	cell	NNS
cord-326027-58whwspe	237	15	throughout	throughout	IN
cord-326027-58whwspe	237	16	the	the	DT
cord-326027-58whwspe	237	17	infection	infection	NN
cord-326027-58whwspe	237	18	cycle	cycle	NN
cord-326027-58whwspe	237	19	for	for	IN
cord-326027-58whwspe	237	20	first	first	JJ
cord-326027-58whwspe	237	21	time	time	NN
cord-326027-58whwspe	237	22	.	.	.
cord-326027-58whwspe	238	1	Animations	animation	NNS
cord-326027-58whwspe	238	2	obtained	obtain	VBD
cord-326027-58whwspe	238	3	show	show	NN
cord-326027-58whwspe	238	4	that	that	IN
cord-326027-58whwspe	238	5	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	238	6	was	be	VBD
cord-326027-58whwspe	238	7	first	first	RB
cord-326027-58whwspe	238	8	detected	detect	VBN
cord-326027-58whwspe	238	9	in	in	IN
cord-326027-58whwspe	238	10	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	238	11	as	as	IN
cord-326027-58whwspe	238	12	several	several	JJ
cord-326027-58whwspe	238	13	discrete	discrete	JJ
cord-326027-58whwspe	238	14	and	and	CC
cord-326027-58whwspe	238	15	bright	bright	JJ
cord-326027-58whwspe	238	16	spots	spot	NNS
cord-326027-58whwspe	238	17	representing	represent	VBG
cord-326027-58whwspe	238	18	initial	initial	JJ
cord-326027-58whwspe	238	19	viral	viral	JJ
cord-326027-58whwspe	238	20	assembly	assembly	NN
cord-326027-58whwspe	238	21	sites	site	NNS
cord-326027-58whwspe	238	22	.	.	.
cord-326027-58whwspe	239	1	As	as	IN
cord-326027-58whwspe	239	2	infection	infection	NN
cord-326027-58whwspe	239	3	progresses	progress	VBZ
cord-326027-58whwspe	239	4	these	these	DT
cord-326027-58whwspe	239	5	initial	initial	JJ
cord-326027-58whwspe	239	6	assembly	assembly	NN
cord-326027-58whwspe	239	7	sites	site	NNS
cord-326027-58whwspe	239	8	migrated	migrate	VBD
cord-326027-58whwspe	239	9	to	to	IN
cord-326027-58whwspe	239	10	a	a	DT
cord-326027-58whwspe	239	11	perinuclear	perinuclear	JJ
cord-326027-58whwspe	239	12	area	area	NN
cord-326027-58whwspe	239	13	,	,	,
cord-326027-58whwspe	239	14	fused	fuse	VBN
cord-326027-58whwspe	239	15	and	and	CC
cord-326027-58whwspe	239	16	finally	finally	RB
cord-326027-58whwspe	239	17	formed	form	VBD
cord-326027-58whwspe	239	18	a	a	DT
cord-326027-58whwspe	239	19	single	single	JJ
cord-326027-58whwspe	239	20	large	large	JJ
cord-326027-58whwspe	239	21	accumulation	accumulation	NN
cord-326027-58whwspe	239	22	of	of	IN
cord-326027-58whwspe	239	23	p54-EGFP	p54-egfp	NN
cord-326027-58whwspe	239	24	per	per	IN
cord-326027-58whwspe	239	25	cell	cell	NN
cord-326027-58whwspe	239	26	.	.	.
cord-326027-58whwspe	240	1	Similar	similar	JJ
cord-326027-58whwspe	240	2	assembly	assembly	NN
cord-326027-58whwspe	240	3	sites	site	NNS
cord-326027-58whwspe	240	4	were	be	VBD
cord-326027-58whwspe	240	5	previously	previously	RB
cord-326027-58whwspe	240	6	described	describe	VBN
cord-326027-58whwspe	240	7	as	as	IN
cord-326027-58whwspe	240	8	viral	viral	JJ
cord-326027-58whwspe	240	9	antigens	antigen	NNS
cord-326027-58whwspe	240	10	inclusions	inclusion	NNS
cord-326027-58whwspe	240	11	containing	contain	VBG
cord-326027-58whwspe	240	12	viral	viral	JJ
cord-326027-58whwspe	240	13	precursors	precursor	NNS
cord-326027-58whwspe	240	14	which	which	WDT
cord-326027-58whwspe	240	15	remained	remain	VBD
cord-326027-58whwspe	240	16	tightly	tightly	RB
cord-326027-58whwspe	240	17	associated	associate	VBN
cord-326027-58whwspe	240	18	to	to	IN
cord-326027-58whwspe	240	19	microtubules	microtubule	NNS
cord-326027-58whwspe	240	20	(	(	-LRB-
cord-326027-58whwspe	240	21	Carvalho	Carvalho	NNP
cord-326027-58whwspe	240	22	et	et	NNP
cord-326027-58whwspe	240	23	al	al	NNP
cord-326027-58whwspe	240	24	.	.	NNP
cord-326027-58whwspe	240	25	,	,	,
cord-326027-58whwspe	240	26	1988	1988	CD
cord-326027-58whwspe	240	27	)	)	-RRB-
cord-326027-58whwspe	240	28	.	.	.
cord-326027-58whwspe	241	1	Indeed	indeed	RB
cord-326027-58whwspe	241	2	,	,	,
cord-326027-58whwspe	241	3	microtubule	microtubule	NN
cord-326027-58whwspe	241	4	associated	associate	VBN
cord-326027-58whwspe	241	5	motors	motor	NNS
cord-326027-58whwspe	241	6	are	be	VBP
cord-326027-58whwspe	241	7	required	require	VBN
cord-326027-58whwspe	241	8	to	to	TO
cord-326027-58whwspe	241	9	transport	transport	VB
cord-326027-58whwspe	241	10	these	these	DT
cord-326027-58whwspe	241	11	initial	initial	JJ
cord-326027-58whwspe	241	12	assembly	assembly	NN
cord-326027-58whwspe	241	13	sites	site	NNS
cord-326027-58whwspe	241	14	towards	towards	IN
cord-326027-58whwspe	241	15	the	the	DT
cord-326027-58whwspe	241	16	microtubule	microtubule	JJ
cord-326027-58whwspe	241	17	organization	organization	NN
cord-326027-58whwspe	241	18	center	center	NN
cord-326027-58whwspe	241	19	(	(	-LRB-
cord-326027-58whwspe	241	20	MTOC	MTOC	NNP
cord-326027-58whwspe	241	21	)	)	-RRB-
cord-326027-58whwspe	241	22	,	,	,
cord-326027-58whwspe	241	23	where	where	WRB
cord-326027-58whwspe	241	24	final	final	JJ
cord-326027-58whwspe	241	25	ASFV	ASFV	NNP
cord-326027-58whwspe	241	26	factory	factory	NN
cord-326027-58whwspe	241	27	is	be	VBZ
cord-326027-58whwspe	241	28	constituted	constitute	VBN
cord-326027-58whwspe	241	29	(	(	-LRB-
cord-326027-58whwspe	242	1	Alonso	Alonso	NNP
cord-326027-58whwspe	242	2	et	et	NNP
cord-326027-58whwspe	242	3	al	al	NNP
cord-326027-58whwspe	242	4	.	.	.
cord-326027-58whwspe	243	1	,	,	,
cord-326027-58whwspe	243	2	2001	2001	CD
cord-326027-58whwspe	243	3	;	;	:
cord-326027-58whwspe	243	4	Heath	Heath	NNP
cord-326027-58whwspe	243	5	et	et	NNP
cord-326027-58whwspe	243	6	al	al	NNP
cord-326027-58whwspe	243	7	.	.	NNP
cord-326027-58whwspe	243	8	,	,	,
cord-326027-58whwspe	243	9	2001	2001	CD
cord-326027-58whwspe	243	10	)	)	-RRB-
cord-326027-58whwspe	243	11	following	follow	VBG
cord-326027-58whwspe	243	12	viral	viral	JJ
cord-326027-58whwspe	243	13	replication	replication	NN
cord-326027-58whwspe	243	14	and	and	CC
cord-326027-58whwspe	243	15	viral	viral	JJ
cord-326027-58whwspe	243	16	assembly	assembly	NN
cord-326027-58whwspe	243	17	takes	take	VBZ
cord-326027-58whwspe	243	18	place	place	NN
cord-326027-58whwspe	243	19	.	.	.
cord-326027-58whwspe	244	1	First	first	JJ
cord-326027-58whwspe	244	2	steps	step	NNS
cord-326027-58whwspe	244	3	in	in	IN
cord-326027-58whwspe	244	4	ASFV	ASFV	NNP
cord-326027-58whwspe	244	5	assembly	assembly	NN
cord-326027-58whwspe	244	6	consist	consist	VBP
cord-326027-58whwspe	244	7	in	in	IN
cord-326027-58whwspe	244	8	the	the	DT
cord-326027-58whwspe	244	9	modification	modification	NN
cord-326027-58whwspe	244	10	of	of	IN
cord-326027-58whwspe	244	11	ER	ER	NNP
cord-326027-58whwspe	244	12	membranes	membrane	NNS
cord-326027-58whwspe	244	13	Rouiller	Rouiller	NNP
cord-326027-58whwspe	244	14	et	et	NNP
cord-326027-58whwspe	244	15	al	al	NNP
cord-326027-58whwspe	244	16	.	.	NNP
cord-326027-58whwspe	244	17	,	,	,
cord-326027-58whwspe	244	18	1998	1998	CD
cord-326027-58whwspe	244	19	)	)	-RRB-
cord-326027-58whwspe	244	20	.	.	.
cord-326027-58whwspe	245	1	Protein	protein	NN
cord-326027-58whwspe	245	2	p54	p54	NN
cord-326027-58whwspe	245	3	has	have	VBZ
cord-326027-58whwspe	245	4	been	be	VBN
cord-326027-58whwspe	245	5	involved	involve	VBN
cord-326027-58whwspe	245	6	in	in	IN
cord-326027-58whwspe	245	7	the	the	DT
cord-326027-58whwspe	245	8	recruitment	recruitment	NN
cord-326027-58whwspe	245	9	to	to	IN
cord-326027-58whwspe	245	10	the	the	DT
cord-326027-58whwspe	245	11	viral	viral	JJ
cord-326027-58whwspe	245	12	factories	factory	NNS
cord-326027-58whwspe	245	13	and	and	CC
cord-326027-58whwspe	245	14	transformation	transformation	NN
cord-326027-58whwspe	245	15	of	of	IN
cord-326027-58whwspe	245	16	the	the	DT
cord-326027-58whwspe	245	17	ER	er	NN
cord-326027-58whwspe	245	18	-	-	HYPH
cord-326027-58whwspe	245	19	derived	derive	VBN
cord-326027-58whwspe	245	20	envelope	envelope	NN
cord-326027-58whwspe	245	21	precursors	precursor	NNS
cord-326027-58whwspe	245	22	that	that	WDT
cord-326027-58whwspe	245	23	are	be	VBP
cord-326027-58whwspe	245	24	finally	finally	RB
cord-326027-58whwspe	245	25	acquired	acquire	VBN
cord-326027-58whwspe	245	26	by	by	IN
cord-326027-58whwspe	245	27	virions	virion	NNS
cord-326027-58whwspe	245	28	(	(	-LRB-
cord-326027-58whwspe	245	29	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	245	30	et	et	NNP
cord-326027-58whwspe	245	31	al	al	NNP
cord-326027-58whwspe	245	32	.	.	NNP
cord-326027-58whwspe	245	33	,	,	,
cord-326027-58whwspe	245	34	2004	2004	CD
cord-326027-58whwspe	245	35	)	)	-RRB-
cord-326027-58whwspe	245	36	.	.	.
cord-326027-58whwspe	246	1	This	this	DT
cord-326027-58whwspe	246	2	new	new	JJ
cord-326027-58whwspe	246	3	role	role	NN
cord-326027-58whwspe	246	4	for	for	IN
cord-326027-58whwspe	246	5	p54	p54	NN
cord-326027-58whwspe	246	6	in	in	IN
cord-326027-58whwspe	246	7	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	246	8	supports	support	VBZ
cord-326027-58whwspe	246	9	the	the	DT
cord-326027-58whwspe	246	10	selection	selection	NN
cord-326027-58whwspe	246	11	of	of	IN
cord-326027-58whwspe	246	12	p54	p54	NN
cord-326027-58whwspe	246	13	as	as	IN
cord-326027-58whwspe	246	14	viral	viral	JJ
cord-326027-58whwspe	246	15	fusion	fusion	NN
cord-326027-58whwspe	246	16	protein	protein	NN
cord-326027-58whwspe	246	17	and	and	CC
cord-326027-58whwspe	246	18	suggests	suggest	VBZ
cord-326027-58whwspe	246	19	that	that	IN
cord-326027-58whwspe	246	20	studies	study	NNS
cord-326027-58whwspe	246	21	about	about	IN
cord-326027-58whwspe	246	22	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	246	23	trafficking	trafficking	NN
cord-326027-58whwspe	246	24	during	during	IN
cord-326027-58whwspe	246	25	infection	infection	NN
cord-326027-58whwspe	246	26	in	in	IN
cord-326027-58whwspe	246	27	live	live	JJ
cord-326027-58whwspe	246	28	cells	cell	NNS
cord-326027-58whwspe	246	29	would	would	MD
cord-326027-58whwspe	246	30	be	be	VB
cord-326027-58whwspe	246	31	helpful	helpful	JJ
cord-326027-58whwspe	246	32	to	to	TO
cord-326027-58whwspe	246	33	analyze	analyze	VB
cord-326027-58whwspe	246	34	the	the	DT
cord-326027-58whwspe	246	35	acquisition	acquisition	NN
cord-326027-58whwspe	246	36	of	of	IN
cord-326027-58whwspe	246	37	ASFV	ASFV	NNP
cord-326027-58whwspe	246	38	envelopes	envelope	NNS
cord-326027-58whwspe	246	39	from	from	IN
cord-326027-58whwspe	246	40	ER	ER	NNP
cord-326027-58whwspe	246	41	during	during	IN
cord-326027-58whwspe	246	42	virus	virus	NN
cord-326027-58whwspe	246	43	assembly	assembly	NN
cord-326027-58whwspe	246	44	.	.	.
cord-326027-58whwspe	247	1	The	the	DT
cord-326027-58whwspe	247	2	formation	formation	NN
cord-326027-58whwspe	247	3	of	of	IN
cord-326027-58whwspe	247	4	the	the	DT
cord-326027-58whwspe	247	5	viral	viral	JJ
cord-326027-58whwspe	247	6	factory	factory	NN
cord-326027-58whwspe	247	7	often	often	RB
cord-326027-58whwspe	247	8	involves	involve	VBZ
cord-326027-58whwspe	247	9	not	not	RB
cord-326027-58whwspe	247	10	only	only	RB
cord-326027-58whwspe	247	11	host	host	NN
cord-326027-58whwspe	247	12	cytoplasmic	cytoplasmic	JJ
cord-326027-58whwspe	247	13	membranes	membrane	NNS
cord-326027-58whwspe	247	14	and	and	CC
cord-326027-58whwspe	247	15	cytoskeletal	cytoskeletal	JJ
cord-326027-58whwspe	247	16	components	component	NNS
cord-326027-58whwspe	247	17	,	,	,
cord-326027-58whwspe	247	18	but	but	CC
cord-326027-58whwspe	247	19	also	also	RB
cord-326027-58whwspe	247	20	cell	cell	NN
cord-326027-58whwspe	247	21	organelles	organelle	NNS
cord-326027-58whwspe	247	22	such	such	JJ
cord-326027-58whwspe	247	23	as	as	IN
cord-326027-58whwspe	247	24	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	247	25	.	.	.
cord-326027-58whwspe	248	1	These	these	DT
cord-326027-58whwspe	248	2	are	be	VBP
cord-326027-58whwspe	248	3	thought	think	VBN
cord-326027-58whwspe	248	4	to	to	TO
cord-326027-58whwspe	248	5	play	play	VB
cord-326027-58whwspe	248	6	an	an	DT
cord-326027-58whwspe	248	7	important	important	JJ
cord-326027-58whwspe	248	8	role	role	NN
cord-326027-58whwspe	248	9	in	in	IN
cord-326027-58whwspe	248	10	several	several	JJ
cord-326027-58whwspe	248	11	large	large	JJ
cord-326027-58whwspe	248	12	DNA	dna	NN
cord-326027-58whwspe	248	13	enveloped	envelop	VBN
cord-326027-58whwspe	248	14	virus	virus	NN
cord-326027-58whwspe	248	15	infections	infection	NNS
cord-326027-58whwspe	248	16	(	(	-LRB-
cord-326027-58whwspe	248	17	Murata	Murata	NNP
cord-326027-58whwspe	248	18	et	et	NNP
cord-326027-58whwspe	248	19	al	al	NNP
cord-326027-58whwspe	248	20	.	.	NNP
cord-326027-58whwspe	248	21	,	,	,
cord-326027-58whwspe	248	22	2000	2000	CD
cord-326027-58whwspe	248	23	;	;	:
cord-326027-58whwspe	248	24	Novoa	Novoa	NNP
cord-326027-58whwspe	248	25	et	et	NNP
cord-326027-58whwspe	248	26	al	al	NNP
cord-326027-58whwspe	248	27	.	.	.
cord-326027-58whwspe	249	1	,	,	,
cord-326027-58whwspe	249	2	2005	2005	CD
cord-326027-58whwspe	249	3	;	;	:
cord-326027-58whwspe	249	4	Rojo	Rojo	NNP
cord-326027-58whwspe	249	5	et	et	NNP
cord-326027-58whwspe	249	6	al	al	NNP
cord-326027-58whwspe	249	7	.	.	NNP
cord-326027-58whwspe	249	8	,	,	,
cord-326027-58whwspe	249	9	1998	1998	CD
cord-326027-58whwspe	249	10	)	)	-RRB-
cord-326027-58whwspe	249	11	,	,	,
cord-326027-58whwspe	249	12	supplying	supply	VBG
cord-326027-58whwspe	249	13	the	the	DT
cord-326027-58whwspe	249	14	energy	energy	NN
cord-326027-58whwspe	249	15	required	require	VBN
cord-326027-58whwspe	249	16	in	in	IN
cord-326027-58whwspe	249	17	the	the	DT
cord-326027-58whwspe	249	18	morphogenetic	morphogenetic	JJ
cord-326027-58whwspe	249	19	and	and	CC
cord-326027-58whwspe	249	20	assembly	assembly	NN
cord-326027-58whwspe	249	21	processes	process	NNS
cord-326027-58whwspe	249	22	.	.	.
cord-326027-58whwspe	250	1	Our	-PRON-	PRP$
cord-326027-58whwspe	250	2	results	result	NNS
cord-326027-58whwspe	250	3	,	,	,
cord-326027-58whwspe	250	4	obtained	obtain	VBN
cord-326027-58whwspe	250	5	with	with	IN
cord-326027-58whwspe	250	6	live	live	JJ
cord-326027-58whwspe	250	7	ASFV	ASFV	NNP
cord-326027-58whwspe	250	8	infected	infect	VBN
cord-326027-58whwspe	250	9	cells	cell	NNS
cord-326027-58whwspe	250	10	have	have	VBP
cord-326027-58whwspe	250	11	confirmed	confirm	VBN
cord-326027-58whwspe	250	12	these	these	DT
cord-326027-58whwspe	250	13	previous	previous	JJ
cord-326027-58whwspe	250	14	observations	observation	NNS
cord-326027-58whwspe	250	15	in	in	IN
cord-326027-58whwspe	250	16	fixed	fix	VBN
cord-326027-58whwspe	250	17	cells	cell	NNS
cord-326027-58whwspe	250	18	(	(	-LRB-
cord-326027-58whwspe	250	19	Hernaez	Hernaez	NNP
cord-326027-58whwspe	250	20	et	et	FW
cord-326027-58whwspe	250	21	al	al	NNP
cord-326027-58whwspe	250	22	.	.	.
cord-326027-58whwspe	251	1	,	,	,
cord-326027-58whwspe	251	2	2004a	2004a	CD
cord-326027-58whwspe	251	3	;	;	:
cord-326027-58whwspe	251	4	Rojo	Rojo	NNP
cord-326027-58whwspe	251	5	et	et	NNP
cord-326027-58whwspe	251	6	al	al	NNP
cord-326027-58whwspe	251	7	.	.	NNP
cord-326027-58whwspe	251	8	,	,	,
cord-326027-58whwspe	251	9	1998	1998	CD
cord-326027-58whwspe	251	10	)	)	-RRB-
cord-326027-58whwspe	251	11	,	,	,
cord-326027-58whwspe	251	12	which	which	WDT
cord-326027-58whwspe	251	13	showed	show	VBD
cord-326027-58whwspe	251	14	that	that	IN
cord-326027-58whwspe	251	15	ASFV	ASFV	NNP
cord-326027-58whwspe	251	16	infection	infection	NN
cord-326027-58whwspe	251	17	induce	induce	VBP
cord-326027-58whwspe	251	18	the	the	DT
cord-326027-58whwspe	251	19	migration	migration	NN
cord-326027-58whwspe	251	20	of	of	IN
cord-326027-58whwspe	251	21	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	251	22	to	to	IN
cord-326027-58whwspe	251	23	the	the	DT
cord-326027-58whwspe	251	24	perinuclear	perinuclear	NNP
cord-326027-58whwspe	251	25	assembly	assembly	NNP
cord-326027-58whwspe	251	26	sites	site	NNS
cord-326027-58whwspe	251	27	,	,	,
cord-326027-58whwspe	251	28	producing	produce	VBG
cord-326027-58whwspe	251	29	the	the	DT
cord-326027-58whwspe	251	30	clustering	clustering	NN
cord-326027-58whwspe	251	31	of	of	IN
cord-326027-58whwspe	251	32	this	this	DT
cord-326027-58whwspe	251	33	actively	actively	RB
cord-326027-58whwspe	251	34	respiring	respire	VBG
cord-326027-58whwspe	251	35	organelle	organelle	NN
cord-326027-58whwspe	251	36	around	around	IN
cord-326027-58whwspe	251	37	viral	viral	JJ
cord-326027-58whwspe	251	38	factory	factory	NN
cord-326027-58whwspe	251	39	.	.	.
cord-326027-58whwspe	252	1	This	this	DT
cord-326027-58whwspe	252	2	migration	migration	NN
cord-326027-58whwspe	252	3	occurs	occur	VBZ
cord-326027-58whwspe	252	4	since	since	IN
cord-326027-58whwspe	252	5	8	8	CD
cord-326027-58whwspe	252	6	hpi	hpi	NNP
cord-326027-58whwspe	252	7	,	,	,
cord-326027-58whwspe	252	8	before	before	IN
cord-326027-58whwspe	252	9	the	the	DT
cord-326027-58whwspe	252	10	definitive	definitive	JJ
cord-326027-58whwspe	252	11	viral	viral	JJ
cord-326027-58whwspe	252	12	factory	factory	NN
cord-326027-58whwspe	252	13	is	be	VBZ
cord-326027-58whwspe	252	14	fully	fully	RB
cord-326027-58whwspe	252	15	constituted	constitute	VBN
cord-326027-58whwspe	252	16	,	,	,
cord-326027-58whwspe	252	17	suggesting	suggest	VBG
cord-326027-58whwspe	252	18	that	that	IN
cord-326027-58whwspe	252	19	energy	energy	NN
cord-326027-58whwspe	252	20	contribution	contribution	NN
cord-326027-58whwspe	252	21	from	from	IN
cord-326027-58whwspe	252	22	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	252	23	is	be	VBZ
cord-326027-58whwspe	252	24	required	require	VBN
cord-326027-58whwspe	252	25	since	since	IN
cord-326027-58whwspe	252	26	first	first	JJ
cord-326027-58whwspe	252	27	stages	stage	NNS
cord-326027-58whwspe	252	28	of	of	IN
cord-326027-58whwspe	252	29	ASFV	ASFV	NNP
cord-326027-58whwspe	252	30	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	252	31	.	.	.
cord-326027-58whwspe	253	1	Finally	finally	RB
cord-326027-58whwspe	253	2	,	,	,
cord-326027-58whwspe	253	3	we	-PRON-	PRP
cord-326027-58whwspe	253	4	analyzed	analyze	VBD
cord-326027-58whwspe	253	5	the	the	DT
cord-326027-58whwspe	253	6	destruction	destruction	NN
cord-326027-58whwspe	253	7	of	of	IN
cord-326027-58whwspe	253	8	the	the	DT
cord-326027-58whwspe	253	9	ASFV	ASFV	NNP
cord-326027-58whwspe	253	10	infected	infect	VBN
cord-326027-58whwspe	253	11	cell	cell	NN
cord-326027-58whwspe	253	12	.	.	.
cord-326027-58whwspe	254	1	This	this	DT
cord-326027-58whwspe	254	2	process	process	NN
cord-326027-58whwspe	254	3	started	start	VBD
cord-326027-58whwspe	254	4	at	at	IN
cord-326027-58whwspe	254	5	12	12	CD
cord-326027-58whwspe	254	6	hpi	hpi	NNS
cord-326027-58whwspe	254	7	and	and	CC
cord-326027-58whwspe	254	8	usually	usually	RB
cord-326027-58whwspe	254	9	lasted	last	VBD
cord-326027-58whwspe	254	10	about	about	RB
cord-326027-58whwspe	254	11	6	6	CD
cord-326027-58whwspe	254	12	h	h	NN
cord-326027-58whwspe	254	13	,	,	,
cord-326027-58whwspe	254	14	being	be	VBG
cord-326027-58whwspe	254	15	identical	identical	JJ
cord-326027-58whwspe	254	16	to	to	IN
cord-326027-58whwspe	254	17	apoptotic	apoptotic	JJ
cord-326027-58whwspe	254	18	cell	cell	NN
cord-326027-58whwspe	254	19	death	death	NN
cord-326027-58whwspe	254	20	,	,	,
cord-326027-58whwspe	254	21	which	which	WDT
cord-326027-58whwspe	254	22	has	have	VBZ
cord-326027-58whwspe	254	23	been	be	VBN
cord-326027-58whwspe	254	24	demonstrated	demonstrate	VBN
cord-326027-58whwspe	254	25	to	to	TO
cord-326027-58whwspe	254	26	play	play	VB
cord-326027-58whwspe	254	27	a	a	DT
cord-326027-58whwspe	254	28	relevant	relevant	JJ
cord-326027-58whwspe	254	29	role	role	NN
cord-326027-58whwspe	254	30	in	in	IN
cord-326027-58whwspe	254	31	ASFV	ASFV	NNP
cord-326027-58whwspe	254	32	pathogenesis	pathogenesis	NN
cord-326027-58whwspe	254	33	(	(	-LRB-
cord-326027-58whwspe	254	34	Hernaez	Hernaez	NNP
cord-326027-58whwspe	254	35	et	et	FW
cord-326027-58whwspe	254	36	al	al	NNP
cord-326027-58whwspe	255	1	.	.	.
cord-326027-58whwspe	256	1	,	,	,
cord-326027-58whwspe	256	2	2004a	2004a	CD
cord-326027-58whwspe	256	3	(	(	-LRB-
cord-326027-58whwspe	256	4	Hernaez	Hernaez	NNP
cord-326027-58whwspe	256	5	et	et	NNP
cord-326027-58whwspe	256	6	al	al	NNP
cord-326027-58whwspe	256	7	.	.	NNP
cord-326027-58whwspe	256	8	,	,	,
cord-326027-58whwspe	257	1	,	,	,
cord-326027-58whwspe	257	2	2004b	2004b	CD
cord-326027-58whwspe	257	3	Ramiro	Ramiro	NNP
cord-326027-58whwspe	257	4	-	-	HYPH
cord-326027-58whwspe	257	5	Ibanez	Ibanez	NNP
cord-326027-58whwspe	257	6	et	et	NNP
cord-326027-58whwspe	257	7	al	al	NNP
cord-326027-58whwspe	257	8	.	.	NNP
cord-326027-58whwspe	257	9	,	,	,
cord-326027-58whwspe	257	10	1996	1996	CD
cord-326027-58whwspe	257	11	)	)	-RRB-
cord-326027-58whwspe	257	12	.	.	.
cord-326027-58whwspe	258	1	In	in	IN
cord-326027-58whwspe	258	2	live	live	JJ
cord-326027-58whwspe	258	3	cells	cell	NNS
cord-326027-58whwspe	258	4	,	,	,
cord-326027-58whwspe	258	5	it	-PRON-	PRP
cord-326027-58whwspe	258	6	was	be	VBD
cord-326027-58whwspe	258	7	possible	possible	JJ
cord-326027-58whwspe	258	8	to	to	TO
cord-326027-58whwspe	258	9	observe	observe	VB
cord-326027-58whwspe	258	10	host	host	NN
cord-326027-58whwspe	258	11	cell	cell	NN
cord-326027-58whwspe	258	12	membrane	membrane	NN
cord-326027-58whwspe	258	13	blebbing	blebbing	NN
cord-326027-58whwspe	258	14	,	,	,
cord-326027-58whwspe	258	15	resulting	result	VBG
cord-326027-58whwspe	258	16	in	in	IN
cord-326027-58whwspe	258	17	the	the	DT
cord-326027-58whwspe	258	18	formation	formation	NN
cord-326027-58whwspe	258	19	of	of	IN
cord-326027-58whwspe	258	20	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	258	21	containing	contain	VBG
cord-326027-58whwspe	258	22	vesicles	vesicle	NNS
cord-326027-58whwspe	258	23	.	.	.
cord-326027-58whwspe	259	1	This	this	DT
cord-326027-58whwspe	259	2	finding	finding	NN
cord-326027-58whwspe	259	3	raised	raise	VBD
cord-326027-58whwspe	259	4	the	the	DT
cord-326027-58whwspe	259	5	possibility	possibility	NN
cord-326027-58whwspe	259	6	that	that	IN
cord-326027-58whwspe	259	7	these	these	DT
cord-326027-58whwspe	259	8	vesicles	vesicle	NNS
cord-326027-58whwspe	259	9	could	could	MD
cord-326027-58whwspe	259	10	contain	contain	VB
cord-326027-58whwspe	259	11	virus	virus	NN
cord-326027-58whwspe	259	12	particles	particle	NNS
cord-326027-58whwspe	259	13	.	.	.
cord-326027-58whwspe	260	1	Previous	previous	JJ
cord-326027-58whwspe	260	2	publications	publication	NNS
cord-326027-58whwspe	260	3	reported	report	VBD
cord-326027-58whwspe	260	4	that	that	IN
cord-326027-58whwspe	260	5	the	the	DT
cord-326027-58whwspe	260	6	abundant	abundant	JJ
cord-326027-58whwspe	260	7	vesicle	vesicle	NN
cord-326027-58whwspe	260	8	fraction	fraction	NN
cord-326027-58whwspe	260	9	released	release	VBN
cord-326027-58whwspe	260	10	in	in	IN
cord-326027-58whwspe	260	11	last	last	JJ
cord-326027-58whwspe	260	12	ASFV	ASFV	NNP
cord-326027-58whwspe	260	13	infection	infection	NN
cord-326027-58whwspe	260	14	stages	stage	NNS
cord-326027-58whwspe	260	15	contains	contain	VBZ
cord-326027-58whwspe	260	16	virus	virus	NN
cord-326027-58whwspe	260	17	(	(	-LRB-
cord-326027-58whwspe	260	18	Carrascosa	Carrascosa	NNP
cord-326027-58whwspe	260	19	et	et	NNP
cord-326027-58whwspe	260	20	al	al	NNP
cord-326027-58whwspe	260	21	.	.	NNP
cord-326027-58whwspe	260	22	,	,	,
cord-326027-58whwspe	260	23	1985	1985	CD
cord-326027-58whwspe	260	24	)	)	-RRB-
cord-326027-58whwspe	260	25	.	.	.
cord-326027-58whwspe	261	1	Then	then	RB
cord-326027-58whwspe	261	2	,	,	,
cord-326027-58whwspe	261	3	it	-PRON-	PRP
cord-326027-58whwspe	261	4	is	be	VBZ
cord-326027-58whwspe	261	5	conceivable	conceivable	JJ
cord-326027-58whwspe	261	6	that	that	IN
cord-326027-58whwspe	261	7	the	the	DT
cord-326027-58whwspe	261	8	fluorescence	fluorescence	NN
cord-326027-58whwspe	261	9	detected	detect	VBN
cord-326027-58whwspe	261	10	in	in	IN
cord-326027-58whwspe	261	11	the	the	DT
cord-326027-58whwspe	261	12	vesicles	vesicle	NNS
cord-326027-58whwspe	261	13	formed	form	VBN
cord-326027-58whwspe	261	14	at	at	IN
cord-326027-58whwspe	261	15	the	the	DT
cord-326027-58whwspe	261	16	end	end	NN
cord-326027-58whwspe	261	17	of	of	IN
cord-326027-58whwspe	261	18	apoptosis	apoptosis	NN
cord-326027-58whwspe	261	19	(	(	-LRB-
cord-326027-58whwspe	261	20	blebbing	blebbe	VBG
cord-326027-58whwspe	261	21	)	)	-RRB-
cord-326027-58whwspe	261	22	,	,	,
cord-326027-58whwspe	261	23	could	could	MD
cord-326027-58whwspe	261	24	correspond	correspond	VB
cord-326027-58whwspe	261	25	to	to	IN
cord-326027-58whwspe	261	26	GFP	GFP	NNP
cord-326027-58whwspe	261	27	labeled	label	VBN
cord-326027-58whwspe	261	28	virus	virus	NN
cord-326027-58whwspe	261	29	particles	particle	NNS
cord-326027-58whwspe	261	30	.	.	.
cord-326027-58whwspe	262	1	This	this	DT
cord-326027-58whwspe	262	2	vesicle	vesicle	NN
cord-326027-58whwspe	262	3	formation	formation	NN
cord-326027-58whwspe	262	4	observed	observe	VBN
cord-326027-58whwspe	262	5	in	in	IN
cord-326027-58whwspe	262	6	live	live	JJ
cord-326027-58whwspe	262	7	cells	cell	NNS
cord-326027-58whwspe	262	8	is	be	VBZ
cord-326027-58whwspe	262	9	rarely	rarely	RB
cord-326027-58whwspe	262	10	observed	observe	VBN
cord-326027-58whwspe	262	11	using	use	VBG
cord-326027-58whwspe	262	12	common	common	JJ
cord-326027-58whwspe	262	13	fixation	fixation	NN
cord-326027-58whwspe	262	14	methods	method	NNS
cord-326027-58whwspe	262	15	,	,	,
cord-326027-58whwspe	262	16	then	then	RB
cord-326027-58whwspe	262	17	it	-PRON-	PRP
cord-326027-58whwspe	262	18	is	be	VBZ
cord-326027-58whwspe	262	19	not	not	RB
cord-326027-58whwspe	262	20	possible	possible	JJ
cord-326027-58whwspe	262	21	to	to	TO
cord-326027-58whwspe	262	22	observe	observe	VB
cord-326027-58whwspe	262	23	it	-PRON-	PRP
cord-326027-58whwspe	262	24	with	with	IN
cord-326027-58whwspe	262	25	the	the	DT
cord-326027-58whwspe	262	26	parental	parental	JJ
cord-326027-58whwspe	262	27	unlabeled	unlabeled	JJ
cord-326027-58whwspe	262	28	virus	virus	NN
cord-326027-58whwspe	262	29	.	.	.
cord-326027-58whwspe	263	1	In	in	IN
cord-326027-58whwspe	263	2	summary	summary	NN
cord-326027-58whwspe	263	3	,	,	,
cord-326027-58whwspe	263	4	we	-PRON-	PRP
cord-326027-58whwspe	263	5	have	have	VBP
cord-326027-58whwspe	263	6	generated	generate	VBN
cord-326027-58whwspe	263	7	a	a	DT
cord-326027-58whwspe	263	8	fluorescently	fluorescently	RB
cord-326027-58whwspe	263	9	tagged	tag	VBN
cord-326027-58whwspe	263	10	ASFV	ASFV	NNP
cord-326027-58whwspe	263	11	,	,	,
cord-326027-58whwspe	263	12	which	which	WDT
cord-326027-58whwspe	263	13	could	could	MD
cord-326027-58whwspe	263	14	be	be	VB
cord-326027-58whwspe	263	15	a	a	DT
cord-326027-58whwspe	263	16	powerful	powerful	JJ
cord-326027-58whwspe	263	17	tool	tool	NN
cord-326027-58whwspe	263	18	since	since	IN
cord-326027-58whwspe	263	19	it	-PRON-	PRP
cord-326027-58whwspe	263	20	enables	enable	VBZ
cord-326027-58whwspe	263	21	live	live	JJ
cord-326027-58whwspe	263	22	analyses	analysis	NNS
cord-326027-58whwspe	263	23	of	of	IN
cord-326027-58whwspe	263	24	diverse	diverse	JJ
cord-326027-58whwspe	263	25	aspects	aspect	NNS
cord-326027-58whwspe	263	26	of	of	IN
cord-326027-58whwspe	263	27	ASFV	ASFV	NNP
cord-326027-58whwspe	263	28	live	live	JJ
cord-326027-58whwspe	263	29	cycle	cycle	NN
cord-326027-58whwspe	263	30	,	,	,
cord-326027-58whwspe	263	31	including	include	VBG
cord-326027-58whwspe	263	32	viral	viral	JJ
cord-326027-58whwspe	263	33	particles	particle	NNS
cord-326027-58whwspe	263	34	intracellular	intracellular	JJ
cord-326027-58whwspe	263	35	transport	transport	NN
cord-326027-58whwspe	263	36	,	,	,
cord-326027-58whwspe	263	37	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	263	38	and	and	CC
cord-326027-58whwspe	263	39	apoptosis	apoptosis	NN
cord-326027-58whwspe	263	40	of	of	IN
cord-326027-58whwspe	263	41	the	the	DT
cord-326027-58whwspe	263	42	infected	infected	JJ
cord-326027-58whwspe	263	43	cell	cell	NN
cord-326027-58whwspe	263	44	.	.	.
cord-326027-58whwspe	264	1	We	-PRON-	PRP
cord-326027-58whwspe	264	2	presented	present	VBD
cord-326027-58whwspe	264	3	here	here	RB
cord-326027-58whwspe	264	4	,	,	,
cord-326027-58whwspe	264	5	for	for	IN
cord-326027-58whwspe	264	6	the	the	DT
cord-326027-58whwspe	264	7	first	first	JJ
cord-326027-58whwspe	264	8	time	time	NN
cord-326027-58whwspe	264	9	,	,	,
cord-326027-58whwspe	264	10	live	live	JJ
cord-326027-58whwspe	264	11	images	image	NNS
cord-326027-58whwspe	264	12	of	of	IN
cord-326027-58whwspe	264	13	some	some	DT
cord-326027-58whwspe	264	14	of	of	IN
cord-326027-58whwspe	264	15	these	these	DT
cord-326027-58whwspe	264	16	stages	stage	NNS
cord-326027-58whwspe	264	17	in	in	IN
cord-326027-58whwspe	264	18	ASFV	ASFV	NNP
cord-326027-58whwspe	264	19	infection	infection	NN
cord-326027-58whwspe	264	20	and	and	CC
cord-326027-58whwspe	264	21	more	more	RBR
cord-326027-58whwspe	264	22	detailed	detailed	JJ
cord-326027-58whwspe	264	23	information	information	NN
cord-326027-58whwspe	264	24	will	will	MD
cord-326027-58whwspe	264	25	arise	arise	VB
cord-326027-58whwspe	264	26	from	from	IN
cord-326027-58whwspe	264	27	future	future	JJ
cord-326027-58whwspe	264	28	and	and	CC
cord-326027-58whwspe	264	29	novel	novel	JJ
cord-326027-58whwspe	264	30	studies	study	NNS
cord-326027-58whwspe	264	31	of	of	IN
cord-326027-58whwspe	264	32	virus	virus	NN
cord-326027-58whwspe	264	33	entry	entry	NN
cord-326027-58whwspe	264	34	and	and	CC
cord-326027-58whwspe	264	35	egress	egress	NN
cord-326027-58whwspe	264	36	.	.	.
cord-326027-58whwspe	265	1	Vero	Vero	NNP
cord-326027-58whwspe	265	2	cells	cell	NNS
cord-326027-58whwspe	265	3	were	be	VBD
cord-326027-58whwspe	265	4	obtained	obtain	VBN
cord-326027-58whwspe	265	5	from	from	IN
cord-326027-58whwspe	265	6	ECACC	ECACC	NNP
cord-326027-58whwspe	265	7	and	and	CC
cord-326027-58whwspe	265	8	grown	grow	VBN
cord-326027-58whwspe	265	9	at	at	IN
cord-326027-58whwspe	265	10	37	37	CD
cord-326027-58whwspe	265	11	°	°	NN
cord-326027-58whwspe	265	12	C	c	NN
cord-326027-58whwspe	265	13	in	in	IN
cord-326027-58whwspe	265	14	a	a	DT
cord-326027-58whwspe	265	15	5	5	CD
cord-326027-58whwspe	265	16	%	%	NN
cord-326027-58whwspe	265	17	CO	co	NN
cord-326027-58whwspe	265	18	2	2	CD
cord-326027-58whwspe	265	19	atmosphere	atmosphere	NN
cord-326027-58whwspe	265	20	in	in	IN
cord-326027-58whwspe	265	21	Dulbecco	Dulbecco	NNP
cord-326027-58whwspe	265	22	's	's	POS
cord-326027-58whwspe	265	23	modified	modify	VBN
cord-326027-58whwspe	265	24	Eagle	Eagle	NNP
cord-326027-58whwspe	265	25	's	's	POS
cord-326027-58whwspe	265	26	medium	medium	NN
cord-326027-58whwspe	265	27	(	(	-LRB-
cord-326027-58whwspe	265	28	DMEM	DMEM	NNP
cord-326027-58whwspe	265	29	)	)	-RRB-
cord-326027-58whwspe	265	30	supplemented	supplement	VBD
cord-326027-58whwspe	265	31	with	with	IN
cord-326027-58whwspe	265	32	5	5	CD
cord-326027-58whwspe	265	33	%	%	NN
cord-326027-58whwspe	265	34	foetal	foetal	JJ
cord-326027-58whwspe	265	35	bovine	bovine	JJ
cord-326027-58whwspe	265	36	serum	serum	NN
cord-326027-58whwspe	265	37	(	(	-LRB-
cord-326027-58whwspe	265	38	FBS	FBS	NNP
cord-326027-58whwspe	265	39	)	)	-RRB-
cord-326027-58whwspe	265	40	.	.	.
cord-326027-58whwspe	266	1	ASFV	ASFV	NNP
cord-326027-58whwspe	266	2	isolate	isolate	VBP
cord-326027-58whwspe	266	3	BA71V	ba71v	NN
cord-326027-58whwspe	266	4	,	,	,
cord-326027-58whwspe	266	5	adapted	adapt	VBN
cord-326027-58whwspe	266	6	to	to	TO
cord-326027-58whwspe	266	7	grow	grow	VB
cord-326027-58whwspe	266	8	in	in	IN
cord-326027-58whwspe	266	9	Vero	Vero	NNP
cord-326027-58whwspe	266	10	cells	cell	NNS
cord-326027-58whwspe	266	11	,	,	,
cord-326027-58whwspe	266	12	was	be	VBD
cord-326027-58whwspe	266	13	used	use	VBN
cord-326027-58whwspe	266	14	in	in	IN
cord-326027-58whwspe	266	15	infection	infection	NN
cord-326027-58whwspe	266	16	experiments	experiment	NNS
cord-326027-58whwspe	266	17	carried	carry	VBN
cord-326027-58whwspe	266	18	out	out	RP
cord-326027-58whwspe	266	19	at	at	IN
cord-326027-58whwspe	266	20	37	37	CD
cord-326027-58whwspe	266	21	°	°	NN
cord-326027-58whwspe	266	22	C	c	NN
cord-326027-58whwspe	266	23	and	and	CC
cord-326027-58whwspe	266	24	5	5	CD
cord-326027-58whwspe	266	25	%	%	NN
cord-326027-58whwspe	266	26	CO	co	NN
cord-326027-58whwspe	266	27	2	2	CD
cord-326027-58whwspe	266	28	.	.	.
cord-326027-58whwspe	267	1	When	when	WRB
cord-326027-58whwspe	267	2	synchronization	synchronization	NN
cord-326027-58whwspe	267	3	of	of	IN
cord-326027-58whwspe	267	4	infection	infection	NN
cord-326027-58whwspe	267	5	was	be	VBD
cord-326027-58whwspe	267	6	required	require	VBN
cord-326027-58whwspe	267	7	,	,	,
cord-326027-58whwspe	267	8	this	this	DT
cord-326027-58whwspe	267	9	was	be	VBD
cord-326027-58whwspe	267	10	performed	perform	VBN
cord-326027-58whwspe	267	11	for	for	IN
cord-326027-58whwspe	267	12	90	90	CD
cord-326027-58whwspe	267	13	min	min	NN
cord-326027-58whwspe	267	14	at	at	IN
cord-326027-58whwspe	267	15	4	4	CD
cord-326027-58whwspe	267	16	°	°	NN
cord-326027-58whwspe	267	17	C	c	NN
cord-326027-58whwspe	267	18	after	after	IN
cord-326027-58whwspe	267	19	viral	viral	JJ
cord-326027-58whwspe	267	20	inoculum	inoculum	NN
cord-326027-58whwspe	267	21	was	be	VBD
cord-326027-58whwspe	267	22	added	add	VBN
cord-326027-58whwspe	267	23	.	.	.
cord-326027-58whwspe	268	1	In	in	IN
cord-326027-58whwspe	268	2	most	most	JJS
cord-326027-58whwspe	268	3	cases	case	NNS
cord-326027-58whwspe	268	4	,	,	,
cord-326027-58whwspe	268	5	ASFV	ASFV	NNP
cord-326027-58whwspe	268	6	stocks	stock	NNS
cord-326027-58whwspe	268	7	from	from	IN
cord-326027-58whwspe	268	8	culture	culture	NN
cord-326027-58whwspe	268	9	supernatants	supernatant	NNS
cord-326027-58whwspe	268	10	were	be	VBD
cord-326027-58whwspe	268	11	semipurified	semipurifie	VBN
cord-326027-58whwspe	268	12	from	from	IN
cord-326027-58whwspe	268	13	vesicles	vesicle	NNS
cord-326027-58whwspe	268	14	by	by	IN
cord-326027-58whwspe	268	15	ultracentrifugation	ultracentrifugation	NN
cord-326027-58whwspe	268	16	at	at	IN
cord-326027-58whwspe	268	17	40.000	40.000	CD
cord-326027-58whwspe	268	18	×	×	NN
cord-326027-58whwspe	268	19	g	g	NN
cord-326027-58whwspe	268	20	through	through	IN
cord-326027-58whwspe	268	21	a	a	DT
cord-326027-58whwspe	268	22	20	20	CD
cord-326027-58whwspe	268	23	%	%	NN
cord-326027-58whwspe	268	24	(	(	-LRB-
cord-326027-58whwspe	268	25	w	w	NN
cord-326027-58whwspe	268	26	/	/	SYM
cord-326027-58whwspe	268	27	v	v	NN
cord-326027-58whwspe	268	28	)	)	-RRB-
cord-326027-58whwspe	269	1	sucrose	sucrose	NN
cord-326027-58whwspe	269	2	cushion	cushion	NN
cord-326027-58whwspe	269	3	in	in	IN
cord-326027-58whwspe	269	4	PBS	PBS	NNP
cord-326027-58whwspe	269	5	for	for	IN
cord-326027-58whwspe	269	6	1	1	CD
cord-326027-58whwspe	269	7	h	h	NN
cord-326027-58whwspe	269	8	at	at	IN
cord-326027-58whwspe	269	9	4	4	CD
cord-326027-58whwspe	269	10	°	°	NN
cord-326027-58whwspe	269	11	C	c	NN
cord-326027-58whwspe	269	12	.	.	.
cord-326027-58whwspe	270	1	When	when	WRB
cord-326027-58whwspe	270	2	needed	need	VBN
cord-326027-58whwspe	270	3	,	,	,
cord-326027-58whwspe	270	4	ASFV	ASFV	NNP
cord-326027-58whwspe	270	5	particles	particle	NNS
cord-326027-58whwspe	270	6	were	be	VBD
cord-326027-58whwspe	270	7	highly	highly	RB
cord-326027-58whwspe	270	8	purified	purify	VBN
cord-326027-58whwspe	270	9	from	from	IN
cord-326027-58whwspe	270	10	the	the	DT
cord-326027-58whwspe	270	11	extracellular	extracellular	JJ
cord-326027-58whwspe	270	12	medium	medium	NN
cord-326027-58whwspe	270	13	by	by	IN
cord-326027-58whwspe	270	14	Percoll	Percoll	NNP
cord-326027-58whwspe	270	15	equilibrium	equilibrium	NN
cord-326027-58whwspe	270	16	centrifugation	centrifugation	NN
cord-326027-58whwspe	270	17	as	as	IN
cord-326027-58whwspe	270	18	previously	previously	RB
cord-326027-58whwspe	270	19	described	describe	VBN
cord-326027-58whwspe	271	1	(	(	-LRB-
cord-326027-58whwspe	271	2	Carrascosa	Carrascosa	NNP
cord-326027-58whwspe	271	3	et	et	NNP
cord-326027-58whwspe	271	4	al	al	NNP
cord-326027-58whwspe	271	5	.	.	NNP
cord-326027-58whwspe	271	6	,	,	,
cord-326027-58whwspe	271	7	1985	1985	CD
cord-326027-58whwspe	271	8	)	)	-RRB-
cord-326027-58whwspe	271	9	.	.	.
cord-326027-58whwspe	272	1	After	after	IN
cord-326027-58whwspe	272	2	centrifugation	centrifugation	NN
cord-326027-58whwspe	272	3	,	,	,
cord-326027-58whwspe	272	4	Percoll	Percoll	NNP
cord-326027-58whwspe	272	5	gradients	gradient	NNS
cord-326027-58whwspe	272	6	were	be	VBD
cord-326027-58whwspe	272	7	fractionated	fractionate	VBN
cord-326027-58whwspe	272	8	and	and	CC
cord-326027-58whwspe	272	9	fractions	fraction	NNS
cord-326027-58whwspe	272	10	2	2	CD
cord-326027-58whwspe	272	11	to	to	TO
cord-326027-58whwspe	272	12	8	8	CD
cord-326027-58whwspe	272	13	from	from	IN
cord-326027-58whwspe	272	14	Percoll	Percoll	NNP
cord-326027-58whwspe	272	15	gradients	gradient	NNS
cord-326027-58whwspe	272	16	,	,	,
cord-326027-58whwspe	272	17	containing	contain	VBG
cord-326027-58whwspe	272	18	virus	virus	NN
cord-326027-58whwspe	272	19	particle	particle	NN
cord-326027-58whwspe	272	20	essentially	essentially	RB
cord-326027-58whwspe	272	21	free	free	JJ
cord-326027-58whwspe	272	22	of	of	IN
cord-326027-58whwspe	272	23	vesicles	vesicle	NNS
cord-326027-58whwspe	272	24	and	and	CC
cord-326027-58whwspe	272	25	contaminant	contaminant	NN
cord-326027-58whwspe	272	26	membranes	membrane	NNS
cord-326027-58whwspe	272	27	,	,	,
cord-326027-58whwspe	272	28	were	be	VBD
cord-326027-58whwspe	272	29	collected	collect	VBN
cord-326027-58whwspe	272	30	after	after	IN
cord-326027-58whwspe	272	31	sedimentation	sedimentation	NN
cord-326027-58whwspe	272	32	and	and	CC
cord-326027-58whwspe	272	33	gel	gel	NN
cord-326027-58whwspe	272	34	filtered	filter	VBN
cord-326027-58whwspe	272	35	through	through	IN
cord-326027-58whwspe	272	36	Sephacryl	Sephacryl	NNP
cord-326027-58whwspe	273	1	S-1000	S-1000	NNP
cord-326027-58whwspe	274	1	(	(	-LRB-
cord-326027-58whwspe	274	2	Amersham	Amersham	NNP
cord-326027-58whwspe	274	3	)	)	-RRB-
cord-326027-58whwspe	274	4	to	to	TO
cord-326027-58whwspe	274	5	remove	remove	VB
cord-326027-58whwspe	274	6	Percoll	Percoll	NNP
cord-326027-58whwspe	274	7	.	.	.
cord-326027-58whwspe	275	1	The	the	DT
cord-326027-58whwspe	275	2	construction	construction	NN
cord-326027-58whwspe	275	3	of	of	IN
cord-326027-58whwspe	275	4	the	the	DT
cord-326027-58whwspe	275	5	insertion	insertion	NN
cord-326027-58whwspe	275	6	vector	vector	NN
cord-326027-58whwspe	275	7	pINS-54EGFP	pINS-54EGFP	NNP
cord-326027-58whwspe	275	8	involved	involve	VBD
cord-326027-58whwspe	275	9	several	several	JJ
cord-326027-58whwspe	275	10	steps	step	NNS
cord-326027-58whwspe	275	11	.	.	.
cord-326027-58whwspe	276	1	The	the	DT
cord-326027-58whwspe	276	2	expression	expression	NN
cord-326027-58whwspe	276	3	plasmid	plasmid	VBN
cord-326027-58whwspe	276	4	pEGFP	pEGFP	NNP
cord-326027-58whwspe	276	5	-	-	HYPH
cord-326027-58whwspe	276	6	N1	N1	NNP
cord-326027-58whwspe	276	7	(	(	-LRB-
cord-326027-58whwspe	276	8	Clontech	Clontech	NNP
cord-326027-58whwspe	276	9	)	)	-RRB-
cord-326027-58whwspe	276	10	was	be	VBD
cord-326027-58whwspe	276	11	used	use	VBN
cord-326027-58whwspe	276	12	as	as	IN
cord-326027-58whwspe	276	13	template	template	NN
cord-326027-58whwspe	276	14	to	to	TO
cord-326027-58whwspe	276	15	obtain	obtain	VB
cord-326027-58whwspe	276	16	the	the	DT
cord-326027-58whwspe	276	17	EGFP	EGFP	NNP
cord-326027-58whwspe	276	18	coding	coding	NN
cord-326027-58whwspe	276	19	sequence	sequence	NN
cord-326027-58whwspe	276	20	by	by	IN
cord-326027-58whwspe	276	21	PCR	PCR	NNP
cord-326027-58whwspe	276	22	with	with	IN
cord-326027-58whwspe	276	23	specific	specific	JJ
cord-326027-58whwspe	276	24	primers	primer	NNS
cord-326027-58whwspe	276	25	(	(	-LRB-
cord-326027-58whwspe	276	26	5′-CGCGGATCCGC	5′-CGCGGATCCGC	NNP
cord-326027-58whwspe	276	27	-	-	HYPH
cord-326027-58whwspe	276	28	AGTAAAAAAATG-3′	AGTAAAAAAATG-3′	NNP
cord-326027-58whwspe	276	29	and	and	CC
cord-326027-58whwspe	276	30	5′-CGCGGAATTCATGGTGAG	5′-CGCGGAATTCATGGTGAG	NNP
cord-326027-58whwspe	276	31	-	-	HYPH
cord-326027-58whwspe	276	32	CAAGGGC-3′	CAAGGGC-3′	NNP
cord-326027-58whwspe	276	33	)	)	-RRB-
cord-326027-58whwspe	276	34	.	.	.
cord-326027-58whwspe	277	1	A	a	DT
cord-326027-58whwspe	277	2	fragment	fragment	NN
cord-326027-58whwspe	277	3	of	of	IN
cord-326027-58whwspe	277	4	800	800	CD
cord-326027-58whwspe	277	5	pb	pb	NNS
cord-326027-58whwspe	277	6	corresponding	correspond	VBG
cord-326027-58whwspe	277	7	to	to	IN
cord-326027-58whwspe	277	8	p54	p54	NN
cord-326027-58whwspe	277	9	promoter	promoter	NN
cord-326027-58whwspe	277	10	and	and	CC
cord-326027-58whwspe	277	11	complete	complete	JJ
cord-326027-58whwspe	277	12	p54	p54	NN
cord-326027-58whwspe	277	13	coding	coding	NN
cord-326027-58whwspe	277	14	sequence	sequence	NN
cord-326027-58whwspe	277	15	was	be	VBD
cord-326027-58whwspe	277	16	also	also	RB
cord-326027-58whwspe	277	17	obtained	obtain	VBN
cord-326027-58whwspe	277	18	from	from	IN
cord-326027-58whwspe	277	19	BA71V	ba71v	NN
cord-326027-58whwspe	277	20	genome	genome	NN
cord-326027-58whwspe	277	21	by	by	IN
cord-326027-58whwspe	277	22	PCR	PCR	NNP
cord-326027-58whwspe	277	23	with	with	IN
cord-326027-58whwspe	277	24	specific	specific	JJ
cord-326027-58whwspe	277	25	primers	primer	NNS
cord-326027-58whwspe	277	26	(	(	-LRB-
cord-326027-58whwspe	277	27	5′-GCGGGATCCCGTTGTCTAGGTAA-3′and	5′-gcgggatcccgttgtctaggtaa-3′and	CD
cord-326027-58whwspe	277	28	5′-GCGGAATT	5′-GCGGAATT	NNP
cord-326027-58whwspe	277	29	-	-	HYPH
cord-326027-58whwspe	277	30	CCAAGGAGTTTTCT	CCAAGGAGTTTTCT	NNP
cord-326027-58whwspe	277	31	AGG-3′	AGG-3′	NNPS
cord-326027-58whwspe	277	32	)	)	-RRB-
cord-326027-58whwspe	277	33	.	.	.
cord-326027-58whwspe	278	1	In	in	IN
cord-326027-58whwspe	278	2	order	order	NN
cord-326027-58whwspe	278	3	to	to	TO
cord-326027-58whwspe	278	4	generate	generate	VB
cord-326027-58whwspe	278	5	the	the	DT
cord-326027-58whwspe	278	6	vector	vector	NN
cord-326027-58whwspe	278	7	pINS-54GFP	pins-54gfp	NN
cord-326027-58whwspe	278	8	,	,	,
cord-326027-58whwspe	278	9	previous	previous	JJ
cord-326027-58whwspe	278	10	PCR	PCR	NNP
cord-326027-58whwspe	278	11	products	product	NNS
cord-326027-58whwspe	278	12	were	be	VBD
cord-326027-58whwspe	278	13	digested	digest	VBN
cord-326027-58whwspe	278	14	with	with	IN
cord-326027-58whwspe	278	15	EcoRI	ecori	NN
cord-326027-58whwspe	278	16	and	and	CC
cord-326027-58whwspe	278	17	BamHI	bamhi	NN
cord-326027-58whwspe	278	18	and	and	CC
cord-326027-58whwspe	278	19	cloned	clone	VBN
cord-326027-58whwspe	278	20	into	into	IN
cord-326027-58whwspe	278	21	the	the	DT
cord-326027-58whwspe	278	22	4.7	4.7	CD
cord-326027-58whwspe	278	23	kpb	kpb	NN
cord-326027-58whwspe	278	24	fragment	fragment	NN
cord-326027-58whwspe	278	25	resulting	result	VBG
cord-326027-58whwspe	278	26	from	from	IN
cord-326027-58whwspe	278	27	the	the	DT
cord-326027-58whwspe	278	28	digestion	digestion	NN
cord-326027-58whwspe	278	29	of	of	IN
cord-326027-58whwspe	278	30	plasmid	plasmid	NN
cord-326027-58whwspe	278	31	pINS72Gal	pins72gal	NN
cord-326027-58whwspe	279	1	(	(	-LRB-
cord-326027-58whwspe	279	2	Gomez	Gomez	NNP
cord-326027-58whwspe	279	3	-	-	HYPH
cord-326027-58whwspe	279	4	Puertas	Puertas	NNP
cord-326027-58whwspe	279	5	et	et	NNP
cord-326027-58whwspe	279	6	al	al	NNP
cord-326027-58whwspe	279	7	.	.	.
cord-326027-58whwspe	280	1	,	,	,
cord-326027-58whwspe	280	2	1995	1995	CD
cord-326027-58whwspe	280	3	;	;	:
cord-326027-58whwspe	280	4	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	280	5	et	et	NNP
cord-326027-58whwspe	280	6	al	al	NNP
cord-326027-58whwspe	280	7	.	.	NNP
cord-326027-58whwspe	280	8	,	,	,
cord-326027-58whwspe	280	9	1992	1992	CD
cord-326027-58whwspe	280	10	)	)	-RRB-
cord-326027-58whwspe	280	11	with	with	IN
cord-326027-58whwspe	280	12	BamHI	BamHI	NNP
cord-326027-58whwspe	280	13	.	.	.
cord-326027-58whwspe	281	1	The	the	DT
cord-326027-58whwspe	281	2	construction	construction	NN
cord-326027-58whwspe	281	3	of	of	IN
cord-326027-58whwspe	281	4	the	the	DT
cord-326027-58whwspe	281	5	vector	vector	NN
cord-326027-58whwspe	281	6	pΔp54	pδp54	NN
cord-326027-58whwspe	281	7	,	,	,
cord-326027-58whwspe	281	8	used	use	VBN
cord-326027-58whwspe	281	9	to	to	TO
cord-326027-58whwspe	281	10	perform	perform	VB
cord-326027-58whwspe	281	11	the	the	DT
cord-326027-58whwspe	281	12	interruption	interruption	NN
cord-326027-58whwspe	281	13	of	of	IN
cord-326027-58whwspe	281	14	E183L	E183L	NNP
cord-326027-58whwspe	281	15	gene	gene	NN
cord-326027-58whwspe	281	16	,	,	,
cord-326027-58whwspe	281	17	was	be	VBD
cord-326027-58whwspe	281	18	described	describe	VBN
cord-326027-58whwspe	281	19	previously	previously	RB
cord-326027-58whwspe	281	20	(	(	-LRB-
cord-326027-58whwspe	281	21	Rodriguez	Rodriguez	NNP
cord-326027-58whwspe	281	22	et	et	NNP
cord-326027-58whwspe	281	23	al	al	NNP
cord-326027-58whwspe	281	24	.	.	NNP
cord-326027-58whwspe	281	25	,	,	,
cord-326027-58whwspe	281	26	1996	1996	CD
cord-326027-58whwspe	281	27	)	)	-RRB-
cord-326027-58whwspe	281	28	.	.	.
cord-326027-58whwspe	282	1	Briefly	briefly	RB
cord-326027-58whwspe	282	2	,	,	,
cord-326027-58whwspe	282	3	this	this	DT
cord-326027-58whwspe	282	4	vector	vector	NN
cord-326027-58whwspe	282	5	contains	contain	VBZ
cord-326027-58whwspe	282	6	β	β	NN
cord-326027-58whwspe	282	7	-	-	HYPH
cord-326027-58whwspe	282	8	galactosidase	galactosidase	NN
cord-326027-58whwspe	282	9	coding	code	VBG
cord-326027-58whwspe	282	10	sequence	sequence	NN
cord-326027-58whwspe	282	11	under	under	IN
cord-326027-58whwspe	282	12	control	control	NN
cord-326027-58whwspe	282	13	of	of	IN
cord-326027-58whwspe	282	14	p72	p72	NN
cord-326027-58whwspe	282	15	promoter	promoter	NN
cord-326027-58whwspe	282	16	,	,	,
cord-326027-58whwspe	282	17	flanked	flank	VBN
cord-326027-58whwspe	282	18	by	by	IN
cord-326027-58whwspe	282	19	two	two	CD
cord-326027-58whwspe	282	20	fragments	fragment	NNS
cord-326027-58whwspe	282	21	from	from	IN
cord-326027-58whwspe	282	22	E183L	E183L	NNP
cord-326027-58whwspe	282	23	that	that	WDT
cord-326027-58whwspe	282	24	allow	allow	VBP
cord-326027-58whwspe	282	25	homologous	homologous	JJ
cord-326027-58whwspe	282	26	recombination	recombination	NN
cord-326027-58whwspe	282	27	with	with	IN
cord-326027-58whwspe	282	28	this	this	DT
cord-326027-58whwspe	282	29	ORF	ORF	NNP
cord-326027-58whwspe	282	30	in	in	IN
cord-326027-58whwspe	282	31	BA71V	ba71v	NN
cord-326027-58whwspe	282	32	genome	genome	NN
cord-326027-58whwspe	282	33	.	.	.
cord-326027-58whwspe	283	1	These	these	DT
cord-326027-58whwspe	283	2	flanking	flanking	JJ
cord-326027-58whwspe	283	3	sequences	sequence	NNS
cord-326027-58whwspe	283	4	correspond	correspond	VBP
cord-326027-58whwspe	283	5	to	to	IN
cord-326027-58whwspe	283	6	424	424	CD
cord-326027-58whwspe	283	7	pb	pb	NNP
cord-326027-58whwspe	283	8	and	and	CC
cord-326027-58whwspe	283	9	888	888	CD
cord-326027-58whwspe	283	10	pb	pb	NNP
cord-326027-58whwspe	283	11	from	from	IN
cord-326027-58whwspe	283	12	right	right	RB
cord-326027-58whwspe	283	13	and	and	CC
cord-326027-58whwspe	283	14	left	leave	VBD
cord-326027-58whwspe	283	15	extremes	extreme	NNS
cord-326027-58whwspe	283	16	from	from	IN
cord-326027-58whwspe	283	17	p54	p54	NN
cord-326027-58whwspe	283	18	gene	gene	NN
cord-326027-58whwspe	283	19	,	,	,
cord-326027-58whwspe	283	20	respectively	respectively	RB
cord-326027-58whwspe	283	21	.	.	.
cord-326027-58whwspe	284	1	An	an	DT
cord-326027-58whwspe	284	2	African	african	JJ
cord-326027-58whwspe	284	3	swine	swine	NN
cord-326027-58whwspe	284	4	fever	fever	NN
cord-326027-58whwspe	284	5	virus	virus	NN
cord-326027-58whwspe	284	6	recombinant	recombinant	JJ
cord-326027-58whwspe	284	7	expressing	express	VBG
cord-326027-58whwspe	284	8	an	an	DT
cord-326027-58whwspe	284	9	enhanced	enhanced	JJ
cord-326027-58whwspe	284	10	version	version	NN
cord-326027-58whwspe	284	11	of	of	IN
cord-326027-58whwspe	284	12	the	the	DT
cord-326027-58whwspe	284	13	GFP	GFP	NNP
cord-326027-58whwspe	284	14	gene	gene	NN
cord-326027-58whwspe	284	15	(	(	-LRB-
cord-326027-58whwspe	284	16	EGFP	EGFP	NNP
cord-326027-58whwspe	284	17	)	)	-RRB-
cord-326027-58whwspe	284	18	fused	fuse	VBD
cord-326027-58whwspe	284	19	to	to	IN
cord-326027-58whwspe	284	20	the	the	DT
cord-326027-58whwspe	284	21	Cterminus	Cterminus	NNP
cord-326027-58whwspe	284	22	of	of	IN
cord-326027-58whwspe	284	23	the	the	DT
cord-326027-58whwspe	284	24	p54	p54	NN
cord-326027-58whwspe	284	25	coding	coding	NN
cord-326027-58whwspe	284	26	sequence	sequence	NN
cord-326027-58whwspe	284	27	was	be	VBD
cord-326027-58whwspe	284	28	constructed	construct	VBN
cord-326027-58whwspe	284	29	in	in	IN
cord-326027-58whwspe	284	30	two	two	CD
cord-326027-58whwspe	284	31	steps	step	NNS
cord-326027-58whwspe	284	32	:	:	:
cord-326027-58whwspe	285	1	first	first	RB
cord-326027-58whwspe	285	2	,	,	,
cord-326027-58whwspe	285	3	the	the	DT
cord-326027-58whwspe	285	4	insertion	insertion	NN
cord-326027-58whwspe	285	5	of	of	IN
cord-326027-58whwspe	285	6	the	the	DT
cord-326027-58whwspe	285	7	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	285	8	coding	code	VBG
cord-326027-58whwspe	285	9	sequence	sequence	NN
cord-326027-58whwspe	285	10	into	into	IN
cord-326027-58whwspe	285	11	thymidine	thymidine	JJ
cord-326027-58whwspe	285	12	kinase	kinase	NN
cord-326027-58whwspe	285	13	locus	locus	NN
cord-326027-58whwspe	285	14	(	(	-LRB-
cord-326027-58whwspe	285	15	TK	TK	NNP
cord-326027-58whwspe	285	16	)	)	-RRB-
cord-326027-58whwspe	285	17	in	in	IN
cord-326027-58whwspe	285	18	BA71V	ba71v	NN
cord-326027-58whwspe	285	19	genome	genome	NN
cord-326027-58whwspe	285	20	was	be	VBD
cord-326027-58whwspe	285	21	achieved	achieve	VBN
cord-326027-58whwspe	285	22	by	by	IN
cord-326027-58whwspe	285	23	homologous	homologous	JJ
cord-326027-58whwspe	285	24	recombination	recombination	NN
cord-326027-58whwspe	285	25	after	after	IN
cord-326027-58whwspe	285	26	transfection	transfection	NN
cord-326027-58whwspe	285	27	of	of	IN
cord-326027-58whwspe	285	28	Vero	Vero	NNP
cord-326027-58whwspe	285	29	cells	cell	NNS
cord-326027-58whwspe	285	30	with	with	IN
cord-326027-58whwspe	285	31	pINS	pins	CD
cord-326027-58whwspe	285	32	-	-	HYPH
cord-326027-58whwspe	285	33	p54EGFP	p54egfp	NN
cord-326027-58whwspe	285	34	in	in	IN
cord-326027-58whwspe	285	35	the	the	DT
cord-326027-58whwspe	285	36	presence	presence	NN
cord-326027-58whwspe	285	37	of	of	IN
cord-326027-58whwspe	285	38	Fugene	Fugene	NNP
cord-326027-58whwspe	285	39	(	(	-LRB-
cord-326027-58whwspe	285	40	Roche	Roche	NNP
cord-326027-58whwspe	285	41	)	)	-RRB-
cord-326027-58whwspe	285	42	,	,	,
cord-326027-58whwspe	285	43	according	accord	VBG
cord-326027-58whwspe	285	44	to	to	IN
cord-326027-58whwspe	285	45	manufacturer	manufacturer	NN
cord-326027-58whwspe	285	46	's	's	POS
cord-326027-58whwspe	285	47	instructions	instruction	NNS
cord-326027-58whwspe	285	48	.	.	.
cord-326027-58whwspe	286	1	Eight	eight	CD
cord-326027-58whwspe	286	2	to	to	TO
cord-326027-58whwspe	286	3	16	16	CD
cord-326027-58whwspe	286	4	h	h	NN
cord-326027-58whwspe	286	5	after	after	IN
cord-326027-58whwspe	286	6	transfection	transfection	NN
cord-326027-58whwspe	286	7	,	,	,
cord-326027-58whwspe	286	8	cells	cell	NNS
cord-326027-58whwspe	286	9	were	be	VBD
cord-326027-58whwspe	286	10	infected	infect	VBN
cord-326027-58whwspe	286	11	with	with	IN
cord-326027-58whwspe	286	12	BA71V	ba71v	NN
cord-326027-58whwspe	286	13	at	at	IN
cord-326027-58whwspe	286	14	a	a	DT
cord-326027-58whwspe	286	15	moi	moi	NN
cord-326027-58whwspe	286	16	of	of	IN
cord-326027-58whwspe	286	17	0.1	0.1	CD
cord-326027-58whwspe	286	18	pfu/	pfu/	CD
cord-326027-58whwspe	286	19	cell	cell	NN
cord-326027-58whwspe	286	20	.	.	.
cord-326027-58whwspe	287	1	When	when	WRB
cord-326027-58whwspe	287	2	the	the	DT
cord-326027-58whwspe	287	3	cytophatic	cytophatic	JJ
cord-326027-58whwspe	287	4	effect	effect	NN
cord-326027-58whwspe	287	5	was	be	VBD
cord-326027-58whwspe	287	6	complete	complete	JJ
cord-326027-58whwspe	287	7	(	(	-LRB-
cord-326027-58whwspe	287	8	approximately	approximately	RB
cord-326027-58whwspe	287	9	72	72	CD
cord-326027-58whwspe	287	10	hpi	hpi	NN
cord-326027-58whwspe	287	11	)	)	-RRB-
cord-326027-58whwspe	288	1	the	the	DT
cord-326027-58whwspe	288	2	cultures	culture	NNS
cord-326027-58whwspe	288	3	were	be	VBD
cord-326027-58whwspe	288	4	harvested	harvest	VBN
cord-326027-58whwspe	288	5	and	and	CC
cord-326027-58whwspe	288	6	sonicated	sonicate	VBN
cord-326027-58whwspe	288	7	.	.	.
cord-326027-58whwspe	289	1	These	these	DT
cord-326027-58whwspe	289	2	transfection	transfection	NN
cord-326027-58whwspe	289	3	-	-	HYPH
cord-326027-58whwspe	289	4	infection	infection	NN
cord-326027-58whwspe	289	5	mixtures	mixture	NNS
cord-326027-58whwspe	289	6	were	be	VBD
cord-326027-58whwspe	289	7	used	use	VBN
cord-326027-58whwspe	289	8	to	to	IN
cord-326027-58whwspe	289	9	infected	infected	JJ
cord-326027-58whwspe	289	10	monolayers	monolayer	NNS
cord-326027-58whwspe	289	11	of	of	IN
cord-326027-58whwspe	289	12	Vero	Vero	NNP
cord-326027-58whwspe	289	13	cells	cell	NNS
cord-326027-58whwspe	289	14	seeded	seed	VBN
cord-326027-58whwspe	289	15	onto	onto	IN
cord-326027-58whwspe	289	16	100	100	CD
cord-326027-58whwspe	289	17	mm	mm	NN
cord-326027-58whwspe	289	18	dishes	dish	NNS
cord-326027-58whwspe	289	19	(	(	-LRB-
cord-326027-58whwspe	289	20	Nunc	Nunc	NNP
cord-326027-58whwspe	289	21	)	)	-RRB-
cord-326027-58whwspe	289	22	at	at	IN
cord-326027-58whwspe	289	23	different	different	JJ
cord-326027-58whwspe	289	24	dilutions	dilution	NNS
cord-326027-58whwspe	289	25	.	.	.
cord-326027-58whwspe	290	1	After	after	IN
cord-326027-58whwspe	290	2	5	5	CD
cord-326027-58whwspe	290	3	-	-	SYM
cord-326027-58whwspe	290	4	6	6	CD
cord-326027-58whwspe	290	5	dpi	dpi	NN
cord-326027-58whwspe	290	6	,	,	,
cord-326027-58whwspe	290	7	the	the	DT
cord-326027-58whwspe	290	8	recombinant	recombinant	JJ
cord-326027-58whwspe	290	9	virus	virus	NN
cord-326027-58whwspe	290	10	generated	generate	VBN
cord-326027-58whwspe	290	11	was	be	VBD
cord-326027-58whwspe	290	12	isolated	isolate	VBN
cord-326027-58whwspe	290	13	from	from	IN
cord-326027-58whwspe	290	14	progeny	progeny	JJ
cord-326027-58whwspe	290	15	virus	virus	NN
cord-326027-58whwspe	290	16	by	by	IN
cord-326027-58whwspe	290	17	three	three	CD
cord-326027-58whwspe	290	18	rounds	round	NNS
cord-326027-58whwspe	290	19	of	of	IN
cord-326027-58whwspe	290	20	plaque	plaque	NN
cord-326027-58whwspe	290	21	purification	purification	NN
cord-326027-58whwspe	290	22	on	on	IN
cord-326027-58whwspe	290	23	Vero	Vero	NNP
cord-326027-58whwspe	290	24	cells	cell	NNS
cord-326027-58whwspe	290	25	,	,	,
cord-326027-58whwspe	290	26	during	during	IN
cord-326027-58whwspe	290	27	which	which	WDT
cord-326027-58whwspe	290	28	recombinant	recombinant	JJ
cord-326027-58whwspe	290	29	virus	virus	NN
cord-326027-58whwspe	290	30	plaques	plaque	NNS
cord-326027-58whwspe	290	31	were	be	VBD
cord-326027-58whwspe	290	32	screened	screen	VBN
cord-326027-58whwspe	290	33	for	for	IN
cord-326027-58whwspe	290	34	EGFP	EGFP	NNP
cord-326027-58whwspe	290	35	direct	direct	JJ
cord-326027-58whwspe	290	36	fluorescence	fluorescence	NN
cord-326027-58whwspe	290	37	.	.	.
cord-326027-58whwspe	291	1	The	the	DT
cord-326027-58whwspe	291	2	resulting	result	VBG
cord-326027-58whwspe	291	3	recombinant	recombinant	JJ
cord-326027-58whwspe	291	4	virus	virus	NN
cord-326027-58whwspe	291	5	was	be	VBD
cord-326027-58whwspe	291	6	named	name	VBN
cord-326027-58whwspe	291	7	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	291	8	.	.	.
cord-326027-58whwspe	292	1	Additional	additional	JJ
cord-326027-58whwspe	292	2	second	second	JJ
cord-326027-58whwspe	292	3	step	step	NN
cord-326027-58whwspe	292	4	consisted	consist	VBN
cord-326027-58whwspe	292	5	in	in	IN
cord-326027-58whwspe	292	6	the	the	DT
cord-326027-58whwspe	292	7	inactivation	inactivation	NN
cord-326027-58whwspe	292	8	of	of	IN
cord-326027-58whwspe	292	9	the	the	DT
cord-326027-58whwspe	292	10	original	original	JJ
cord-326027-58whwspe	292	11	gene	gene	NN
cord-326027-58whwspe	292	12	of	of	IN
cord-326027-58whwspe	292	13	p54	p54	CD
cord-326027-58whwspe	292	14	(	(	-LRB-
cord-326027-58whwspe	292	15	E183L	E183L	NNP
cord-326027-58whwspe	292	16	)	)	-RRB-
cord-326027-58whwspe	292	17	in	in	IN
cord-326027-58whwspe	292	18	the	the	DT
cord-326027-58whwspe	292	19	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	292	20	genome	genome	NN
cord-326027-58whwspe	292	21	to	to	TO
cord-326027-58whwspe	292	22	finally	finally	RB
cord-326027-58whwspe	292	23	generate	generate	VB
cord-326027-58whwspe	292	24	the	the	DT
cord-326027-58whwspe	292	25	recombinant	recombinant	JJ
cord-326027-58whwspe	292	26	virus	virus	NN
cord-326027-58whwspe	292	27	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	292	28	.	.	.
cord-326027-58whwspe	293	1	The	the	DT
cord-326027-58whwspe	293	2	transfection	transfection	NN
cord-326027-58whwspe	293	3	of	of	IN
cord-326027-58whwspe	293	4	Vero	Vero	NNP
cord-326027-58whwspe	293	5	cells	cell	NNS
cord-326027-58whwspe	293	6	with	with	IN
cord-326027-58whwspe	293	7	pΔp54	pδp54	NN
cord-326027-58whwspe	293	8	and	and	CC
cord-326027-58whwspe	293	9	following	follow	VBG
cord-326027-58whwspe	293	10	infection	infection	NN
cord-326027-58whwspe	293	11	with	with	IN
cord-326027-58whwspe	293	12	B54GFP-1	B54GFP-1	NNP
cord-326027-58whwspe	293	13	allowed	allow	VBD
cord-326027-58whwspe	293	14	the	the	DT
cord-326027-58whwspe	293	15	insertion	insertion	NN
cord-326027-58whwspe	293	16	of	of	IN
cord-326027-58whwspe	293	17	the	the	DT
cord-326027-58whwspe	293	18	β	β	NN
cord-326027-58whwspe	293	19	-	-	HYPH
cord-326027-58whwspe	293	20	galactosidase	galactosidase	NN
cord-326027-58whwspe	293	21	coding	code	VBG
cord-326027-58whwspe	293	22	sequence	sequence	NN
cord-326027-58whwspe	293	23	downstream	downstream	RB
cord-326027-58whwspe	293	24	of	of	IN
cord-326027-58whwspe	293	25	the	the	DT
cord-326027-58whwspe	293	26	strong	strong	JJ
cord-326027-58whwspe	293	27	p73	p73	CD
cord-326027-58whwspe	293	28	promoter	promoter	NN
cord-326027-58whwspe	293	29	within	within	IN
cord-326027-58whwspe	293	30	E183L	E183L	NNP
cord-326027-58whwspe	293	31	gene	gene	NN
cord-326027-58whwspe	293	32	.	.	.
cord-326027-58whwspe	294	1	Isolation	isolation	NN
cord-326027-58whwspe	294	2	of	of	IN
cord-326027-58whwspe	294	3	B54GFP-2	B54GFP-2	NNP
cord-326027-58whwspe	294	4	was	be	VBD
cord-326027-58whwspe	294	5	performed	perform	VBN
cord-326027-58whwspe	294	6	as	as	IN
cord-326027-58whwspe	294	7	described	describe	VBN
cord-326027-58whwspe	294	8	above	above	RB
cord-326027-58whwspe	294	9	,	,	,
cord-326027-58whwspe	294	10	screening	screen	VBG
cord-326027-58whwspe	294	11	recombinant	recombinant	JJ
cord-326027-58whwspe	294	12	virus	virus	NN
cord-326027-58whwspe	294	13	plaques	plaque	NNS
cord-326027-58whwspe	294	14	for	for	IN
cord-326027-58whwspe	294	15	EGFP	EGFP	NNP
cord-326027-58whwspe	294	16	fluorescence	fluorescence	NN
cord-326027-58whwspe	294	17	and	and	CC
cord-326027-58whwspe	294	18	expression	expression	NN
cord-326027-58whwspe	294	19	of	of	IN
cord-326027-58whwspe	294	20	β	β	NN
cord-326027-58whwspe	294	21	-	-	HYPH
cord-326027-58whwspe	294	22	galactosidase	galactosidase	NNP
cord-326027-58whwspe	295	1	(	(	-LRB-
cord-326027-58whwspe	295	2	Gomez	Gomez	NNP
cord-326027-58whwspe	295	3	-	-	HYPH
cord-326027-58whwspe	295	4	Puertas	Puertas	NNP
cord-326027-58whwspe	295	5	et	et	NNP
cord-326027-58whwspe	295	6	al	al	NNP
cord-326027-58whwspe	295	7	.	.	NNP
cord-326027-58whwspe	295	8	,	,	,
cord-326027-58whwspe	295	9	1995	1995	CD
cord-326027-58whwspe	295	10	)	)	-RRB-
cord-326027-58whwspe	295	11	.	.	.
cord-326027-58whwspe	296	1	The	the	DT
cord-326027-58whwspe	296	2	genomic	genomic	JJ
cord-326027-58whwspe	296	3	structure	structure	NN
cord-326027-58whwspe	296	4	of	of	IN
cord-326027-58whwspe	296	5	these	these	DT
cord-326027-58whwspe	296	6	recombinant	recombinant	JJ
cord-326027-58whwspe	296	7	viruses	virus	NNS
cord-326027-58whwspe	296	8	was	be	VBD
cord-326027-58whwspe	296	9	confirmed	confirm	VBN
cord-326027-58whwspe	296	10	by	by	IN
cord-326027-58whwspe	296	11	DNA	dna	NN
cord-326027-58whwspe	296	12	hybridization	hybridization	NN
cord-326027-58whwspe	296	13	analysis	analysis	NN
cord-326027-58whwspe	296	14	and	and	CC
cord-326027-58whwspe	296	15	could	could	MD
cord-326027-58whwspe	296	16	be	be	VB
cord-326027-58whwspe	296	17	observed	observe	VBN
cord-326027-58whwspe	296	18	in	in	IN
cord-326027-58whwspe	296	19	Figs	Figs	NNP
cord-326027-58whwspe	296	20	.	.	.
cord-326027-58whwspe	297	1	1a	1a	NNP
cord-326027-58whwspe	297	2	and	and	CC
cord-326027-58whwspe	297	3	b	b	NN
cord-326027-58whwspe	297	4	.	.	.
cord-326027-58whwspe	298	1	Briefly	briefly	RB
cord-326027-58whwspe	298	2	,	,	,
cord-326027-58whwspe	298	3	DNA	DNA	NNP
cord-326027-58whwspe	298	4	from	from	IN
cord-326027-58whwspe	298	5	parental	parental	JJ
cord-326027-58whwspe	298	6	and	and	CC
cord-326027-58whwspe	298	7	recombinant	recombinant	JJ
cord-326027-58whwspe	298	8	viruses	virus	NNS
cord-326027-58whwspe	298	9	were	be	VBD
cord-326027-58whwspe	298	10	obtained	obtain	VBN
cord-326027-58whwspe	298	11	and	and	CC
cord-326027-58whwspe	298	12	purified	purify	VBN
cord-326027-58whwspe	298	13	as	as	IN
cord-326027-58whwspe	298	14	previously	previously	RB
cord-326027-58whwspe	298	15	described	describe	VBN
cord-326027-58whwspe	298	16	(	(	-LRB-
cord-326027-58whwspe	298	17	Esposito	Esposito	NNP
cord-326027-58whwspe	298	18	et	et	NNP
cord-326027-58whwspe	298	19	al	al	NNP
cord-326027-58whwspe	298	20	.	.	NNP
cord-326027-58whwspe	298	21	,	,	,
cord-326027-58whwspe	298	22	1981	1981	CD
cord-326027-58whwspe	298	23	)	)	-RRB-
cord-326027-58whwspe	298	24	.	.	.
cord-326027-58whwspe	299	1	Resulting	result	VBG
cord-326027-58whwspe	299	2	fragments	fragment	NNS
cord-326027-58whwspe	299	3	,	,	,
cord-326027-58whwspe	299	4	after	after	IN
cord-326027-58whwspe	299	5	Eco	Eco	NNP
cord-326027-58whwspe	299	6	RI	RI	NNP
cord-326027-58whwspe	299	7	digestion	digestion	NN
cord-326027-58whwspe	299	8	,	,	,
cord-326027-58whwspe	299	9	were	be	VBD
cord-326027-58whwspe	299	10	resolved	resolve	VBN
cord-326027-58whwspe	299	11	by	by	IN
cord-326027-58whwspe	299	12	agarose	agarose	JJ
cord-326027-58whwspe	299	13	gel	gel	NN
cord-326027-58whwspe	299	14	electrophoresis	electrophoresis	NN
cord-326027-58whwspe	299	15	and	and	CC
cord-326027-58whwspe	299	16	then	then	RB
cord-326027-58whwspe	299	17	blotted	blot	VBD
cord-326027-58whwspe	299	18	onto	onto	IN
cord-326027-58whwspe	299	19	uncharged	uncharged	JJ
cord-326027-58whwspe	299	20	Nylon	nylon	NN
cord-326027-58whwspe	299	21	membranes	membrane	NNS
cord-326027-58whwspe	299	22	(	(	-LRB-
cord-326027-58whwspe	299	23	Amersham	Amersham	NNP
cord-326027-58whwspe	299	24	)	)	-RRB-
cord-326027-58whwspe	299	25	.	.	.
cord-326027-58whwspe	300	1	Membranes	membrane	NNS
cord-326027-58whwspe	300	2	were	be	VBD
cord-326027-58whwspe	300	3	probed	probe	VBN
cord-326027-58whwspe	300	4	with	with	IN
cord-326027-58whwspe	300	5	a	a	DT
cord-326027-58whwspe	300	6	digoxigenin-11-dUTP	digoxigenin-11-dutp	NN
cord-326027-58whwspe	300	7	labeled	label	VBN
cord-326027-58whwspe	300	8	DNA	dna	NN
cord-326027-58whwspe	300	9	fragment	fragment	NN
cord-326027-58whwspe	300	10	corresponding	correspond	VBG
cord-326027-58whwspe	300	11	to	to	IN
cord-326027-58whwspe	300	12	the	the	DT
cord-326027-58whwspe	300	13	complete	complete	JJ
cord-326027-58whwspe	300	14	p54	p54	NN
cord-326027-58whwspe	300	15	coding	coding	NN
cord-326027-58whwspe	300	16	sequence	sequence	NN
cord-326027-58whwspe	300	17	.	.	.
cord-326027-58whwspe	301	1	Final	final	JJ
cord-326027-58whwspe	301	2	detection	detection	NN
cord-326027-58whwspe	301	3	of	of	IN
cord-326027-58whwspe	301	4	digoxigenin	digoxigenin	NNP
cord-326027-58whwspe	301	5	was	be	VBD
cord-326027-58whwspe	301	6	performed	perform	VBN
cord-326027-58whwspe	301	7	with	with	IN
cord-326027-58whwspe	301	8	CSPD	CSPD	NNP
cord-326027-58whwspe	301	9	(	(	-LRB-
cord-326027-58whwspe	301	10	Roche	Roche	NNP
cord-326027-58whwspe	301	11	)	)	-RRB-
cord-326027-58whwspe	301	12	after	after	IN
cord-326027-58whwspe	301	13	probing	probe	VBG
cord-326027-58whwspe	301	14	the	the	DT
cord-326027-58whwspe	301	15	membrane	membrane	NN
cord-326027-58whwspe	301	16	with	with	IN
cord-326027-58whwspe	301	17	specific	specific	JJ
cord-326027-58whwspe	301	18	monoclonal	monoclonal	JJ
cord-326027-58whwspe	301	19	antibody	antibody	NN
cord-326027-58whwspe	301	20	anti	anti	JJ
cord-326027-58whwspe	301	21	-	-	JJ
cord-326027-58whwspe	301	22	digoxigenin	digoxigenin	JJ
cord-326027-58whwspe	301	23	(	(	-LRB-
cord-326027-58whwspe	301	24	Roche	Roche	NNP
cord-326027-58whwspe	301	25	)	)	-RRB-
cord-326027-58whwspe	301	26	.	.	.
cord-326027-58whwspe	302	1	Preconfluent	Preconfluent	NNP
cord-326027-58whwspe	302	2	monolayers	monolayer	NNS
cord-326027-58whwspe	302	3	of	of	IN
cord-326027-58whwspe	302	4	Vero	Vero	NNP
cord-326027-58whwspe	302	5	cells	cell	NNS
cord-326027-58whwspe	302	6	were	be	VBD
cord-326027-58whwspe	302	7	infected	infect	VBN
cord-326027-58whwspe	302	8	with	with	IN
cord-326027-58whwspe	302	9	either	either	DT
cord-326027-58whwspe	302	10	recombinant	recombinant	JJ
cord-326027-58whwspe	302	11	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	302	12	or	or	CC
cord-326027-58whwspe	302	13	parental	parental	JJ
cord-326027-58whwspe	302	14	BA71V	ba71v	NN
cord-326027-58whwspe	302	15	at	at	IN
cord-326027-58whwspe	302	16	0.1	0.1	CD
cord-326027-58whwspe	302	17	pfu/	pfu/	CD
cord-326027-58whwspe	302	18	cell	cell	NN
cord-326027-58whwspe	302	19	.	.	.
cord-326027-58whwspe	303	1	After	after	IN
cord-326027-58whwspe	303	2	90	90	CD
cord-326027-58whwspe	303	3	min	min	NN
cord-326027-58whwspe	303	4	,	,	,
cord-326027-58whwspe	303	5	inoculum	inoculum	NN
cord-326027-58whwspe	303	6	was	be	VBD
cord-326027-58whwspe	303	7	removed	remove	VBN
cord-326027-58whwspe	303	8	and	and	CC
cord-326027-58whwspe	303	9	cells	cell	NNS
cord-326027-58whwspe	303	10	were	be	VBD
cord-326027-58whwspe	303	11	washed	wash	VBN
cord-326027-58whwspe	303	12	with	with	IN
cord-326027-58whwspe	303	13	fresh	fresh	JJ
cord-326027-58whwspe	303	14	DMEM	DMEM	NNP
cord-326027-58whwspe	303	15	and	and	CC
cord-326027-58whwspe	303	16	overlaid	overlay	VBN
cord-326027-58whwspe	303	17	with	with	IN
cord-326027-58whwspe	303	18	DMEM	DMEM	NNP
cord-326027-58whwspe	303	19	supplemented	supplement	VBN
cord-326027-58whwspe	303	20	with	with	IN
cord-326027-58whwspe	303	21	2	2	CD
cord-326027-58whwspe	303	22	%	%	NN
cord-326027-58whwspe	303	23	FBS	FBS	NNP
cord-326027-58whwspe	303	24	.	.	.
cord-326027-58whwspe	304	1	Infected	infected	JJ
cord-326027-58whwspe	304	2	cells	cell	NNS
cord-326027-58whwspe	304	3	with	with	IN
cord-326027-58whwspe	304	4	their	-PRON-	PRP$
cord-326027-58whwspe	304	5	culture	culture	NN
cord-326027-58whwspe	304	6	supernatants	supernatant	NNS
cord-326027-58whwspe	304	7	were	be	VBD
cord-326027-58whwspe	304	8	harvested	harvest	VBN
cord-326027-58whwspe	304	9	at	at	IN
cord-326027-58whwspe	304	10	different	different	JJ
cord-326027-58whwspe	304	11	times	time	NNS
cord-326027-58whwspe	304	12	postinfection	postinfection	NN
cord-326027-58whwspe	304	13	(	(	-LRB-
cord-326027-58whwspe	304	14	0	0	CD
cord-326027-58whwspe	304	15	,	,	,
cord-326027-58whwspe	304	16	12	12	CD
cord-326027-58whwspe	304	17	,	,	,
cord-326027-58whwspe	304	18	24	24	CD
cord-326027-58whwspe	304	19	,	,	,
cord-326027-58whwspe	304	20	48	48	CD
cord-326027-58whwspe	304	21	and	and	CC
cord-326027-58whwspe	304	22	72	72	CD
cord-326027-58whwspe	304	23	hpi	hpi	NNP
cord-326027-58whwspe	304	24	)	)	-RRB-
cord-326027-58whwspe	304	25	and	and	CC
cord-326027-58whwspe	304	26	centrifuged	centrifuge	VBD
cord-326027-58whwspe	304	27	at	at	IN
cord-326027-58whwspe	304	28	3000	3000	CD
cord-326027-58whwspe	304	29	×	×	NN
cord-326027-58whwspe	304	30	g	g	NN
cord-326027-58whwspe	304	31	for	for	IN
cord-326027-58whwspe	304	32	10	10	CD
cord-326027-58whwspe	304	33	min	min	NN
cord-326027-58whwspe	304	34	.	.	.
cord-326027-58whwspe	305	1	Cell	cell	NN
cord-326027-58whwspe	305	2	pellets	pellet	NNS
cord-326027-58whwspe	305	3	was	be	VBD
cord-326027-58whwspe	305	4	resuspended	resuspend	VBN
cord-326027-58whwspe	305	5	in	in	IN
cord-326027-58whwspe	305	6	DMEM	DMEM	NNP
cord-326027-58whwspe	305	7	.	.	.
cord-326027-58whwspe	306	1	Both	both	DT
cord-326027-58whwspe	306	2	the	the	DT
cord-326027-58whwspe	306	3	cellular	cellular	JJ
cord-326027-58whwspe	306	4	fraction	fraction	NN
cord-326027-58whwspe	306	5	and	and	CC
cord-326027-58whwspe	306	6	the	the	DT
cord-326027-58whwspe	306	7	supernatant	supernatant	NN
cord-326027-58whwspe	306	8	were	be	VBD
cord-326027-58whwspe	306	9	sonicated	sonicate	VBN
cord-326027-58whwspe	306	10	and	and	CC
cord-326027-58whwspe	306	11	separately	separately	RB
cord-326027-58whwspe	306	12	titrated	titrate	VBN
cord-326027-58whwspe	306	13	by	by	IN
cord-326027-58whwspe	306	14	plaque	plaque	NN
cord-326027-58whwspe	306	15	assay	assay	NN
cord-326027-58whwspe	306	16	to	to	TO
cord-326027-58whwspe	306	17	determine	determine	VB
cord-326027-58whwspe	306	18	the	the	DT
cord-326027-58whwspe	306	19	intracellular	intracellular	JJ
cord-326027-58whwspe	306	20	and	and	CC
cord-326027-58whwspe	306	21	extracellular	extracellular	JJ
cord-326027-58whwspe	306	22	virus	virus	NN
cord-326027-58whwspe	306	23	production	production	NN
cord-326027-58whwspe	306	24	respectively	respectively	RB
cord-326027-58whwspe	306	25	.	.	.
cord-326027-58whwspe	307	1	Plaques	plaque	NNS
cord-326027-58whwspe	307	2	assays	assay	NNS
cord-326027-58whwspe	307	3	were	be	VBD
cord-326027-58whwspe	307	4	performed	perform	VBN
cord-326027-58whwspe	307	5	as	as	IN
cord-326027-58whwspe	307	6	described	describe	VBN
cord-326027-58whwspe	307	7	previously	previously	RB
cord-326027-58whwspe	307	8	(	(	-LRB-
cord-326027-58whwspe	307	9	Gomez	Gomez	NNP
cord-326027-58whwspe	307	10	-	-	HYPH
cord-326027-58whwspe	307	11	Puertas	Puertas	NNP
cord-326027-58whwspe	307	12	et	et	NNP
cord-326027-58whwspe	307	13	al	al	NNP
cord-326027-58whwspe	307	14	.	.	NNP
cord-326027-58whwspe	307	15	,	,	,
cord-326027-58whwspe	307	16	1995	1995	CD
cord-326027-58whwspe	307	17	)	)	-RRB-
cord-326027-58whwspe	307	18	.	.	.
cord-326027-58whwspe	308	1	When	when	WRB
cord-326027-58whwspe	308	2	isolation	isolation	NN
cord-326027-58whwspe	308	3	of	of	IN
cord-326027-58whwspe	308	4	recombinant	recombinant	JJ
cord-326027-58whwspe	308	5	virus	virus	NN
cord-326027-58whwspe	308	6	plaques	plaque	NNS
cord-326027-58whwspe	308	7	was	be	VBD
cord-326027-58whwspe	308	8	desired	desire	VBN
cord-326027-58whwspe	308	9	,	,	,
cord-326027-58whwspe	308	10	detection	detection	NN
cord-326027-58whwspe	308	11	of	of	IN
cord-326027-58whwspe	308	12	EGFP	EGFP	NNP
cord-326027-58whwspe	308	13	fluorescence	fluorescence	NN
cord-326027-58whwspe	308	14	and	and	CC
cord-326027-58whwspe	308	15	expression	expression	NN
cord-326027-58whwspe	308	16	of	of	IN
cord-326027-58whwspe	308	17	β	β	NN
cord-326027-58whwspe	308	18	-	-	HYPH
cord-326027-58whwspe	308	19	galactosidase	galactosidase	NN
cord-326027-58whwspe	308	20	were	be	VBD
cord-326027-58whwspe	308	21	performed	perform	VBN
cord-326027-58whwspe	308	22	at	at	IN
cord-326027-58whwspe	308	23	4	4	CD
cord-326027-58whwspe	308	24	and	and	CC
cord-326027-58whwspe	308	25	6	6	CD
cord-326027-58whwspe	308	26	days	day	NNS
cord-326027-58whwspe	308	27	postinfection	postinfection	NN
cord-326027-58whwspe	308	28	respectively	respectively	RB
cord-326027-58whwspe	308	29	.	.	.
cord-326027-58whwspe	309	1	Vero	Vero	NNP
cord-326027-58whwspe	309	2	cells	cell	NNS
cord-326027-58whwspe	309	3	preconfluent	preconfluent	NN
cord-326027-58whwspe	309	4	monolayers	monolayer	NNS
cord-326027-58whwspe	309	5	seeded	seed	VBD
cord-326027-58whwspe	309	6	on	on	IN
cord-326027-58whwspe	309	7	6-well	6-well	CD
cord-326027-58whwspe	309	8	plates	plate	NNS
cord-326027-58whwspe	309	9	were	be	VBD
cord-326027-58whwspe	309	10	infected	infect	VBN
cord-326027-58whwspe	309	11	with	with	IN
cord-326027-58whwspe	309	12	BA71V	ba71v	NN
cord-326027-58whwspe	309	13	or	or	CC
cord-326027-58whwspe	309	14	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	309	15	at	at	IN
cord-326027-58whwspe	309	16	a	a	DT
cord-326027-58whwspe	309	17	moi	moi	NN
cord-326027-58whwspe	309	18	of	of	IN
cord-326027-58whwspe	309	19	5	5	CD
cord-326027-58whwspe	309	20	pfu	pfu	NN
cord-326027-58whwspe	309	21	/	/	SYM
cord-326027-58whwspe	309	22	cell	cell	NN
cord-326027-58whwspe	309	23	.	.	.
cord-326027-58whwspe	310	1	When	when	WRB
cord-326027-58whwspe	310	2	inhibition	inhibition	NN
cord-326027-58whwspe	310	3	of	of	IN
cord-326027-58whwspe	310	4	late	late	JJ
cord-326027-58whwspe	310	5	viral	viral	JJ
cord-326027-58whwspe	310	6	protein	protein	NN
cord-326027-58whwspe	310	7	synthesis	synthesis	NN
cord-326027-58whwspe	310	8	was	be	VBD
cord-326027-58whwspe	310	9	required	require	VBN
cord-326027-58whwspe	310	10	,	,	,
cord-326027-58whwspe	310	11	cells	cell	NNS
cord-326027-58whwspe	310	12	were	be	VBD
cord-326027-58whwspe	310	13	incubated	incubate	VBN
cord-326027-58whwspe	310	14	with	with	IN
cord-326027-58whwspe	310	15	cytosine	cytosine	NN
cord-326027-58whwspe	310	16	β	β	NN
cord-326027-58whwspe	310	17	-	-	HYPH
cord-326027-58whwspe	310	18	arabinofuranoside	arabinofuranoside	NNP
cord-326027-58whwspe	310	19	(	(	-LRB-
cord-326027-58whwspe	310	20	Ara	Ara	NNP
cord-326027-58whwspe	310	21	-	-	HYPH
cord-326027-58whwspe	310	22	C	C	NNP
cord-326027-58whwspe	310	23	)	)	-RRB-
cord-326027-58whwspe	311	1	50	50	CD
cord-326027-58whwspe	312	1	μg	μg	NNP
cord-326027-58whwspe	312	2	/	/	SYM
cord-326027-58whwspe	312	3	ml	ml	NN
cord-326027-58whwspe	312	4	at	at	IN
cord-326027-58whwspe	312	5	3	3	CD
cord-326027-58whwspe	312	6	hpi	hpi	NNP
cord-326027-58whwspe	312	7	.	.	.
cord-326027-58whwspe	313	1	In	in	IN
cord-326027-58whwspe	313	2	all	all	DT
cord-326027-58whwspe	313	3	cases	case	NNS
cord-326027-58whwspe	313	4	,	,	,
cord-326027-58whwspe	313	5	cells	cell	NNS
cord-326027-58whwspe	313	6	were	be	VBD
cord-326027-58whwspe	313	7	washed	wash	VBN
cord-326027-58whwspe	313	8	with	with	IN
cord-326027-58whwspe	313	9	PBS	PBS	NNP
cord-326027-58whwspe	313	10	and	and	CC
cord-326027-58whwspe	313	11	harvested	harvest	VBN
cord-326027-58whwspe	313	12	at	at	IN
cord-326027-58whwspe	313	13	0	0	CD
cord-326027-58whwspe	313	14	,	,	,
cord-326027-58whwspe	313	15	4	4	CD
cord-326027-58whwspe	313	16	,	,	,
cord-326027-58whwspe	313	17	8	8	CD
cord-326027-58whwspe	313	18	,	,	,
cord-326027-58whwspe	313	19	12	12	CD
cord-326027-58whwspe	313	20	and	and	CC
cord-326027-58whwspe	313	21	24	24	CD
cord-326027-58whwspe	313	22	hpi	hpi	NNS
cord-326027-58whwspe	313	23	and	and	CC
cord-326027-58whwspe	313	24	lysed	lyse	VBN
cord-326027-58whwspe	313	25	in	in	IN
cord-326027-58whwspe	313	26	RIPA	RIPA	NNP
cord-326027-58whwspe	313	27	buffer	buffer	NN
cord-326027-58whwspe	313	28	.	.	.
cord-326027-58whwspe	314	1	After	after	IN
cord-326027-58whwspe	314	2	centrifugation	centrifugation	NN
cord-326027-58whwspe	314	3	at	at	IN
cord-326027-58whwspe	314	4	10,000	10,000	CD
cord-326027-58whwspe	314	5	×	×	NN
cord-326027-58whwspe	314	6	g	g	NN
cord-326027-58whwspe	314	7	for	for	IN
cord-326027-58whwspe	314	8	20	20	CD
cord-326027-58whwspe	314	9	min	min	NN
cord-326027-58whwspe	314	10	,	,	,
cord-326027-58whwspe	314	11	proteins	protein	NNS
cord-326027-58whwspe	314	12	from	from	IN
cord-326027-58whwspe	314	13	cleared	clear	VBN
cord-326027-58whwspe	314	14	lysates	lysate	NNS
cord-326027-58whwspe	314	15	were	be	VBD
cord-326027-58whwspe	314	16	electrophoresed	electrophorese	VBN
cord-326027-58whwspe	314	17	in	in	IN
cord-326027-58whwspe	314	18	12	12	CD
cord-326027-58whwspe	314	19	%	%	NN
cord-326027-58whwspe	314	20	SDS	sds	NN
cord-326027-58whwspe	314	21	-	-	HYPH
cord-326027-58whwspe	314	22	polyacrylamide	polyacrylamide	NN
cord-326027-58whwspe	314	23	gels	gel	NNS
cord-326027-58whwspe	314	24	and	and	CC
cord-326027-58whwspe	314	25	transferred	transfer	VBN
cord-326027-58whwspe	314	26	to	to	IN
cord-326027-58whwspe	314	27	nitrocelluose	nitrocelluose	JJ
cord-326027-58whwspe	314	28	membranes	membrane	NNS
cord-326027-58whwspe	314	29	(	(	-LRB-
cord-326027-58whwspe	314	30	BioRad	BioRad	NNP
cord-326027-58whwspe	314	31	)	)	-RRB-
cord-326027-58whwspe	314	32	.	.	.
cord-326027-58whwspe	315	1	Membranes	membrane	NNS
cord-326027-58whwspe	315	2	were	be	VBD
cord-326027-58whwspe	315	3	incubated	incubate	VBN
cord-326027-58whwspe	315	4	for	for	IN
cord-326027-58whwspe	315	5	2	2	CD
cord-326027-58whwspe	315	6	h	h	NN
cord-326027-58whwspe	315	7	at	at	IN
cord-326027-58whwspe	315	8	room	room	NN
cord-326027-58whwspe	315	9	temperature	temperature	NN
cord-326027-58whwspe	315	10	in	in	IN
cord-326027-58whwspe	315	11	PBS	PBS	NNP
cord-326027-58whwspe	315	12	containing	contain	VBG
cord-326027-58whwspe	315	13	5	5	CD
cord-326027-58whwspe	315	14	%	%	NN
cord-326027-58whwspe	315	15	non	non	JJ
cord-326027-58whwspe	315	16	-	-	JJ
cord-326027-58whwspe	315	17	fat	fat	JJ
cord-326027-58whwspe	315	18	dry	dry	JJ
cord-326027-58whwspe	315	19	milk	milk	NN
cord-326027-58whwspe	315	20	and	and	CC
cord-326027-58whwspe	315	21	then	then	RB
cord-326027-58whwspe	315	22	probed	probe	VBN
cord-326027-58whwspe	315	23	with	with	IN
cord-326027-58whwspe	315	24	corresponding	correspond	VBG
cord-326027-58whwspe	315	25	primary	primary	JJ
cord-326027-58whwspe	315	26	antibodies	antibody	NNS
cord-326027-58whwspe	315	27	:	:	:
cord-326027-58whwspe	315	28	monoclonal	monoclonal	JJ
cord-326027-58whwspe	315	29	antibody	antibody	NN
cord-326027-58whwspe	315	30	anti	anti	NNP
cord-326027-58whwspe	315	31	p30	p30	NNP
cord-326027-58whwspe	315	32	diluted	dilute	VBD
cord-326027-58whwspe	315	33	1:500	1:500	CD
cord-326027-58whwspe	315	34	,	,	,
cord-326027-58whwspe	315	35	monoclonal	monoclonal	JJ
cord-326027-58whwspe	315	36	antibody	antibody	NN
cord-326027-58whwspe	315	37	anti	anti	JJ
cord-326027-58whwspe	315	38	-	-	JJ
cord-326027-58whwspe	315	39	GFP	gfp	JJ
cord-326027-58whwspe	315	40	diluted	dilute	VBN
cord-326027-58whwspe	315	41	1:2500	1:2500	CD
cord-326027-58whwspe	315	42	(	(	-LRB-
cord-326027-58whwspe	315	43	Clontech	Clontech	NNP
cord-326027-58whwspe	315	44	)	)	-RRB-
cord-326027-58whwspe	315	45	,	,	,
cord-326027-58whwspe	315	46	and	and	CC
cord-326027-58whwspe	315	47	specific	specific	JJ
cord-326027-58whwspe	315	48	serum	serum	NN
cord-326027-58whwspe	315	49	against	against	IN
cord-326027-58whwspe	315	50	p54	p54	NN
cord-326027-58whwspe	315	51	raised	raise	VBN
cord-326027-58whwspe	315	52	in	in	IN
cord-326027-58whwspe	315	53	rabbit	rabbit	NN
cord-326027-58whwspe	315	54	diluted	dilute	VBN
cord-326027-58whwspe	315	55	1:1000	1:1000	NNS
cord-326027-58whwspe	315	56	,	,	,
cord-326027-58whwspe	315	57	for	for	IN
cord-326027-58whwspe	315	58	1	1	CD
cord-326027-58whwspe	315	59	h	h	NN
cord-326027-58whwspe	315	60	at	at	IN
cord-326027-58whwspe	315	61	room	room	NN
cord-326027-58whwspe	315	62	temperature	temperature	NN
cord-326027-58whwspe	315	63	in	in	IN
cord-326027-58whwspe	315	64	PBS	PBS	NNP
cord-326027-58whwspe	315	65	containing	contain	VBG
cord-326027-58whwspe	315	66	0.05	0.05	CD
cord-326027-58whwspe	315	67	%	%	NN
cord-326027-58whwspe	315	68	Tween	Tween	NNP
cord-326027-58whwspe	315	69	20	20	CD
cord-326027-58whwspe	315	70	(	(	-LRB-
cord-326027-58whwspe	315	71	PBS	PBS	NNP
cord-326027-58whwspe	315	72	-	-	HYPH
cord-326027-58whwspe	315	73	T	T	NNP
cord-326027-58whwspe	315	74	)	)	-RRB-
cord-326027-58whwspe	315	75	.	.	.
cord-326027-58whwspe	316	1	After	after	IN
cord-326027-58whwspe	316	2	extensive	extensive	JJ
cord-326027-58whwspe	316	3	washing	washing	NN
cord-326027-58whwspe	316	4	with	with	IN
cord-326027-58whwspe	316	5	PBS	PBS	NNP
cord-326027-58whwspe	316	6	-	-	HYPH
cord-326027-58whwspe	316	7	T	T	NNP
cord-326027-58whwspe	316	8	,	,	,
cord-326027-58whwspe	316	9	membranes	membrane	NNS
cord-326027-58whwspe	316	10	were	be	VBD
cord-326027-58whwspe	316	11	incubated	incubate	VBN
cord-326027-58whwspe	316	12	with	with	IN
cord-326027-58whwspe	316	13	anti	anti	JJ
cord-326027-58whwspe	316	14	mouse	mouse	NN
cord-326027-58whwspe	316	15	IgG	IgG	NNP
cord-326027-58whwspe	316	16	antibody	antibody	NN
cord-326027-58whwspe	316	17	(	(	-LRB-
cord-326027-58whwspe	316	18	diluted	diluted	JJ
cord-326027-58whwspe	316	19	1:5000	1:5000	NNS
cord-326027-58whwspe	316	20	)	)	-RRB-
cord-326027-58whwspe	316	21	conjugated	conjugate	VBN
cord-326027-58whwspe	316	22	to	to	IN
cord-326027-58whwspe	316	23	horseradish	horseradish	NN
cord-326027-58whwspe	316	24	peroxidase	peroxidase	NN
cord-326027-58whwspe	316	25	(	(	-LRB-
cord-326027-58whwspe	316	26	Amersham	Amersham	NNP
cord-326027-58whwspe	316	27	)	)	-RRB-
cord-326027-58whwspe	316	28	or	or	CC
cord-326027-58whwspe	316	29	anti	anti	JJ
cord-326027-58whwspe	316	30	rabbit	rabbit	NN
cord-326027-58whwspe	316	31	IgG	igg	NN
cord-326027-58whwspe	316	32	(	(	-LRB-
cord-326027-58whwspe	316	33	diluted	diluted	JJ
cord-326027-58whwspe	316	34	1:4000	1:4000	CD
cord-326027-58whwspe	316	35	)	)	-RRB-
cord-326027-58whwspe	316	36	conjugated	conjugate	VBN
cord-326027-58whwspe	316	37	to	to	IN
cord-326027-58whwspe	316	38	horseradish	horseradish	NN
cord-326027-58whwspe	316	39	peroxidase	peroxidase	NN
cord-326027-58whwspe	316	40	(	(	-LRB-
cord-326027-58whwspe	316	41	Amersham	Amersham	NNP
cord-326027-58whwspe	316	42	)	)	-RRB-
cord-326027-58whwspe	316	43	.	.	.
cord-326027-58whwspe	317	1	In	in	IN
cord-326027-58whwspe	317	2	other	other	JJ
cord-326027-58whwspe	317	3	experiments	experiment	NNS
cord-326027-58whwspe	317	4	,	,	,
cord-326027-58whwspe	317	5	proteins	protein	NNS
cord-326027-58whwspe	317	6	from	from	IN
cord-326027-58whwspe	317	7	ASFV	ASFV	NNP
cord-326027-58whwspe	317	8	highly	highly	RB
cord-326027-58whwspe	317	9	purified	purify	VBN
cord-326027-58whwspe	317	10	particles	particle	NNS
cord-326027-58whwspe	317	11	were	be	VBD
cord-326027-58whwspe	317	12	analyzed	analyze	VBN
cord-326027-58whwspe	317	13	by	by	IN
cord-326027-58whwspe	317	14	Western	western	JJ
cord-326027-58whwspe	317	15	blotting	blotting	NN
cord-326027-58whwspe	317	16	in	in	IN
cord-326027-58whwspe	317	17	the	the	DT
cord-326027-58whwspe	317	18	same	same	JJ
cord-326027-58whwspe	317	19	way	way	NN
cord-326027-58whwspe	317	20	,	,	,
cord-326027-58whwspe	317	21	but	but	CC
cord-326027-58whwspe	317	22	membranes	membrane	NNS
cord-326027-58whwspe	317	23	were	be	VBD
cord-326027-58whwspe	317	24	probed	probe	VBN
cord-326027-58whwspe	317	25	with	with	IN
cord-326027-58whwspe	317	26	monoclonal	monoclonal	JJ
cord-326027-58whwspe	317	27	anti	anti	JJ
cord-326027-58whwspe	317	28	-	-	JJ
cord-326027-58whwspe	317	29	GFP	gfp	JJ
cord-326027-58whwspe	317	30	antibody	antibody	NN
cord-326027-58whwspe	317	31	,	,	,
cord-326027-58whwspe	317	32	monoclonal	monoclonal	JJ
cord-326027-58whwspe	317	33	anti	anti	JJ
cord-326027-58whwspe	317	34	-	-	JJ
cord-326027-58whwspe	317	35	p72	p72	JJ
cord-326027-58whwspe	317	36	antibody	antibody	NN
cord-326027-58whwspe	317	37	and	and	CC
cord-326027-58whwspe	317	38	polyclonal	polyclonal	JJ
cord-326027-58whwspe	317	39	specific	specific	JJ
cord-326027-58whwspe	317	40	antibody	antibody	NN
cord-326027-58whwspe	317	41	anti	anti	JJ
cord-326027-58whwspe	317	42	-	-	JJ
cord-326027-58whwspe	317	43	p54	p54	JJ
cord-326027-58whwspe	317	44	raised	raise	VBN
cord-326027-58whwspe	317	45	in	in	IN
cord-326027-58whwspe	317	46	rabbit	rabbit	NN
cord-326027-58whwspe	317	47	(	(	-LRB-
cord-326027-58whwspe	317	48	diluted	dilute	VBN
cord-326027-58whwspe	317	49	1:1000	1:1000	CD
cord-326027-58whwspe	317	50	)	)	-RRB-
cord-326027-58whwspe	317	51	.	.	.
cord-326027-58whwspe	318	1	Anti	anti	JJ
cord-326027-58whwspe	318	2	mouse	mouse	NN
cord-326027-58whwspe	318	3	IgG	IgG	NNP
cord-326027-58whwspe	318	4	antibody	antibody	NN
cord-326027-58whwspe	318	5	(	(	-LRB-
cord-326027-58whwspe	318	6	diluted	diluted	JJ
cord-326027-58whwspe	318	7	1:5000	1:5000	CD
cord-326027-58whwspe	318	8	)	)	-RRB-
cord-326027-58whwspe	318	9	and	and	CC
cord-326027-58whwspe	318	10	anti	anti	JJ
cord-326027-58whwspe	318	11	rabbit	rabbit	NNP
cord-326027-58whwspe	318	12	IgG	IgG	NNP
cord-326027-58whwspe	318	13	antibody	antibody	NNP
cord-326027-58whwspe	318	14	(	(	-LRB-
cord-326027-58whwspe	318	15	diluted	diluted	JJ
cord-326027-58whwspe	318	16	1:4000	1:4000	CD
cord-326027-58whwspe	318	17	)	)	-RRB-
cord-326027-58whwspe	318	18	conjugated	conjugate	VBN
cord-326027-58whwspe	318	19	to	to	IN
cord-326027-58whwspe	318	20	horseradish	horseradish	NN
cord-326027-58whwspe	318	21	peroxidase	peroxidase	NN
cord-326027-58whwspe	318	22	(	(	-LRB-
cord-326027-58whwspe	318	23	Amersham	Amersham	NNP
cord-326027-58whwspe	318	24	)	)	-RRB-
cord-326027-58whwspe	318	25	were	be	VBD
cord-326027-58whwspe	318	26	used	use	VBN
cord-326027-58whwspe	318	27	as	as	IN
cord-326027-58whwspe	318	28	secondary	secondary	JJ
cord-326027-58whwspe	318	29	antibodies	antibody	NNS
cord-326027-58whwspe	318	30	respectively	respectively	RB
cord-326027-58whwspe	318	31	.	.	.
cord-326027-58whwspe	319	1	In	in	IN
cord-326027-58whwspe	319	2	all	all	DT
cord-326027-58whwspe	319	3	cases	case	NNS
cord-326027-58whwspe	319	4	,	,	,
cord-326027-58whwspe	319	5	final	final	JJ
cord-326027-58whwspe	319	6	detection	detection	NN
cord-326027-58whwspe	319	7	on	on	IN
cord-326027-58whwspe	319	8	membranes	membrane	NNS
cord-326027-58whwspe	319	9	was	be	VBD
cord-326027-58whwspe	319	10	performed	perform	VBN
cord-326027-58whwspe	319	11	with	with	IN
cord-326027-58whwspe	319	12	ECL	ECL	NNP
cord-326027-58whwspe	319	13	Western	western	JJ
cord-326027-58whwspe	319	14	detection	detection	NN
cord-326027-58whwspe	319	15	reagent	reagent	NN
cord-326027-58whwspe	319	16	(	(	-LRB-
cord-326027-58whwspe	319	17	Amersham	Amersham	NNP
cord-326027-58whwspe	319	18	)	)	-RRB-
cord-326027-58whwspe	319	19	and	and	CC
cord-326027-58whwspe	319	20	exposure	exposure	NN
cord-326027-58whwspe	319	21	to	to	IN
cord-326027-58whwspe	319	22	X	x	NN
cord-326027-58whwspe	319	23	-	-	NN
cord-326027-58whwspe	319	24	ray	ray	NN
cord-326027-58whwspe	319	25	films	film	NNS
cord-326027-58whwspe	319	26	(	(	-LRB-
cord-326027-58whwspe	319	27	Kodak	Kodak	NNP
cord-326027-58whwspe	319	28	)	)	-RRB-
cord-326027-58whwspe	319	29	.	.	.
cord-326027-58whwspe	320	1	Vero	Vero	NNP
cord-326027-58whwspe	320	2	cells	cell	NNS
cord-326027-58whwspe	320	3	were	be	VBD
cord-326027-58whwspe	320	4	grown	grow	VBN
cord-326027-58whwspe	320	5	on	on	IN
cord-326027-58whwspe	320	6	coverslips	coverslip	NNS
cord-326027-58whwspe	320	7	at	at	IN
cord-326027-58whwspe	320	8	50	50	CD
cord-326027-58whwspe	320	9	-	-	SYM
cord-326027-58whwspe	320	10	60	60	CD
cord-326027-58whwspe	320	11	%	%	NN
cord-326027-58whwspe	320	12	confluency	confluency	NN
cord-326027-58whwspe	320	13	and	and	CC
cord-326027-58whwspe	320	14	then	then	RB
cord-326027-58whwspe	320	15	infected	infect	VBN
cord-326027-58whwspe	320	16	with	with	IN
cord-326027-58whwspe	320	17	BA71V	ba71v	NN
cord-326027-58whwspe	320	18	or	or	CC
cord-326027-58whwspe	320	19	recombinant	recombinant	JJ
cord-326027-58whwspe	320	20	viruses	virus	NNS
cord-326027-58whwspe	320	21	at	at	IN
cord-326027-58whwspe	320	22	different	different	JJ
cord-326027-58whwspe	320	23	moi	moi	NN
cord-326027-58whwspe	320	24	depending	depend	VBG
cord-326027-58whwspe	320	25	on	on	IN
cord-326027-58whwspe	320	26	the	the	DT
cord-326027-58whwspe	320	27	experiment	experiment	NN
cord-326027-58whwspe	320	28	.	.	.
cord-326027-58whwspe	321	1	At	at	IN
cord-326027-58whwspe	321	2	desired	desire	VBN
cord-326027-58whwspe	321	3	time	time	NN
cord-326027-58whwspe	321	4	postinfection	postinfection	NN
cord-326027-58whwspe	321	5	cells	cell	NNS
cord-326027-58whwspe	321	6	were	be	VBD
cord-326027-58whwspe	321	7	rinsed	rinse	VBN
cord-326027-58whwspe	321	8	twice	twice	RB
cord-326027-58whwspe	321	9	with	with	IN
cord-326027-58whwspe	321	10	PBS	PBS	NNP
cord-326027-58whwspe	321	11	and	and	CC
cord-326027-58whwspe	321	12	fixed	fix	VBN
cord-326027-58whwspe	321	13	with	with	IN
cord-326027-58whwspe	321	14	PBS	PBS	NNP
cord-326027-58whwspe	321	15	containing	contain	VBG
cord-326027-58whwspe	321	16	4	4	CD
cord-326027-58whwspe	321	17	%	%	NN
cord-326027-58whwspe	321	18	paraformaldehyde	paraformaldehyde	NNP
cord-326027-58whwspe	321	19	for	for	IN
cord-326027-58whwspe	321	20	10	10	CD
cord-326027-58whwspe	321	21	min	min	NN
cord-326027-58whwspe	321	22	at	at	IN
cord-326027-58whwspe	321	23	room	room	NN
cord-326027-58whwspe	321	24	temperature	temperature	NN
cord-326027-58whwspe	321	25	.	.	.
cord-326027-58whwspe	322	1	When	when	WRB
cord-326027-58whwspe	322	2	permeabilization	permeabilization	NN
cord-326027-58whwspe	322	3	was	be	VBD
cord-326027-58whwspe	322	4	required	require	VBN
cord-326027-58whwspe	322	5	,	,	,
cord-326027-58whwspe	322	6	cells	cell	NNS
cord-326027-58whwspe	322	7	were	be	VBD
cord-326027-58whwspe	322	8	incubated	incubate	VBN
cord-326027-58whwspe	322	9	in	in	IN
cord-326027-58whwspe	322	10	presence	presence	NN
cord-326027-58whwspe	322	11	of	of	IN
cord-326027-58whwspe	322	12	0.1	0.1	CD
cord-326027-58whwspe	322	13	%	%	NN
cord-326027-58whwspe	322	14	Triton	Triton	NNP
cord-326027-58whwspe	322	15	X-100	x-100	RB
cord-326027-58whwspe	322	16	for	for	IN
cord-326027-58whwspe	322	17	15	15	CD
cord-326027-58whwspe	322	18	min	min	NN
cord-326027-58whwspe	322	19	at	at	IN
cord-326027-58whwspe	322	20	room	room	NN
cord-326027-58whwspe	322	21	temperature	temperature	NN
cord-326027-58whwspe	322	22	.	.	.
cord-326027-58whwspe	323	1	A	a	DT
cord-326027-58whwspe	323	2	monospecific	monospecific	JJ
cord-326027-58whwspe	323	3	antiserum	antiserum	NN
cord-326027-58whwspe	323	4	against	against	IN
cord-326027-58whwspe	323	5	p54	p54	NN
cord-326027-58whwspe	323	6	was	be	VBD
cord-326027-58whwspe	323	7	raised	raise	VBN
cord-326027-58whwspe	323	8	in	in	IN
cord-326027-58whwspe	323	9	rabbit	rabbit	NN
cord-326027-58whwspe	323	10	and	and	CC
cord-326027-58whwspe	323	11	used	use	VBD
cord-326027-58whwspe	323	12	to	to	TO
cord-326027-58whwspe	323	13	visualize	visualize	VB
cord-326027-58whwspe	323	14	p54	p54	CD
cord-326027-58whwspe	323	15	(	(	-LRB-
cord-326027-58whwspe	323	16	diluted	dilute	VBN
cord-326027-58whwspe	323	17	1:300	1:300	CD
cord-326027-58whwspe	323	18	)	)	-RRB-
cord-326027-58whwspe	323	19	.	.	.
cord-326027-58whwspe	324	1	To	to	TO
cord-326027-58whwspe	324	2	detect	detect	VB
cord-326027-58whwspe	324	3	tubulin	tubulin	JJ
cord-326027-58whwspe	324	4	a	a	DT
cord-326027-58whwspe	324	5	monoclonal	monoclonal	JJ
cord-326027-58whwspe	324	6	antibody	antibody	NN
cord-326027-58whwspe	324	7	against	against	IN
cord-326027-58whwspe	324	8	β	β	NN
cord-326027-58whwspe	324	9	-	-	HYPH
cord-326027-58whwspe	324	10	tubulin	tubulin	JJ
cord-326027-58whwspe	324	11	(	(	-LRB-
cord-326027-58whwspe	324	12	Sigma	Sigma	NNP
cord-326027-58whwspe	324	13	)	)	-RRB-
cord-326027-58whwspe	324	14	was	be	VBD
cord-326027-58whwspe	324	15	used	use	VBN
cord-326027-58whwspe	324	16	(	(	-LRB-
cord-326027-58whwspe	324	17	diluted	dilute	VBN
cord-326027-58whwspe	324	18	1:200	1:200	CD
cord-326027-58whwspe	324	19	)	)	-RRB-
cord-326027-58whwspe	324	20	.	.	.
cord-326027-58whwspe	325	1	Secondary	secondary	JJ
cord-326027-58whwspe	325	2	antibodies	antibody	NNS
cord-326027-58whwspe	325	3	used	use	VBD
cord-326027-58whwspe	325	4	were	be	VBD
cord-326027-58whwspe	325	5	an	an	DT
cord-326027-58whwspe	325	6	anti	anti	JJ
cord-326027-58whwspe	325	7	rabbit	rabbit	NN
cord-326027-58whwspe	325	8	IgG	IgG	NNP
cord-326027-58whwspe	325	9	antibody	antibody	NN
cord-326027-58whwspe	325	10	conjugated	conjugate	VBN
cord-326027-58whwspe	325	11	to	to	IN
cord-326027-58whwspe	325	12	Alexa	Alexa	NNP
cord-326027-58whwspe	325	13	488	488	CD
cord-326027-58whwspe	325	14	fluor	fluor	NNP
cord-326027-58whwspe	325	15	(	(	-LRB-
cord-326027-58whwspe	325	16	Molecular	Molecular	NNP
cord-326027-58whwspe	325	17	Probes	Probes	NNPS
cord-326027-58whwspe	325	18	)	)	-RRB-
cord-326027-58whwspe	325	19	and	and	CC
cord-326027-58whwspe	325	20	anti	anti	JJ
cord-326027-58whwspe	325	21	mouse	mouse	NNP
cord-326027-58whwspe	325	22	IgG	IgG	NNP
cord-326027-58whwspe	325	23	antibody	antibody	NN
cord-326027-58whwspe	325	24	conjugated	conjugate	VBN
cord-326027-58whwspe	325	25	to	to	IN
cord-326027-58whwspe	325	26	Alexa	Alexa	NNP
cord-326027-58whwspe	325	27	fluor	fluor	NNP
cord-326027-58whwspe	325	28	647	647	CD
cord-326027-58whwspe	326	1	(	(	-LRB-
cord-326027-58whwspe	326	2	Molecular	Molecular	NNP
cord-326027-58whwspe	326	3	Probes	Probes	NNPS
cord-326027-58whwspe	326	4	)	)	-RRB-
cord-326027-58whwspe	326	5	.	.	.
cord-326027-58whwspe	327	1	Specificity	specificity	NN
cord-326027-58whwspe	327	2	of	of	IN
cord-326027-58whwspe	327	3	labeling	labeling	NN
cord-326027-58whwspe	327	4	and	and	CC
cord-326027-58whwspe	327	5	absence	absence	NN
cord-326027-58whwspe	327	6	of	of	IN
cord-326027-58whwspe	327	7	signal	signal	NN
cord-326027-58whwspe	327	8	crossover	crossover	NN
cord-326027-58whwspe	327	9	were	be	VBD
cord-326027-58whwspe	327	10	determined	determine	VBN
cord-326027-58whwspe	327	11	by	by	IN
cord-326027-58whwspe	327	12	examination	examination	NN
cord-326027-58whwspe	327	13	of	of	IN
cord-326027-58whwspe	327	14	single	single	JJ
cord-326027-58whwspe	327	15	labeled	label	VBN
cord-326027-58whwspe	327	16	control	control	NN
cord-326027-58whwspe	327	17	samples	sample	NNS
cord-326027-58whwspe	327	18	.	.	.
cord-326027-58whwspe	328	1	p54-EGFP	p54-EGFP	NNP
cord-326027-58whwspe	328	2	was	be	VBD
cord-326027-58whwspe	328	3	directly	directly	RB
cord-326027-58whwspe	328	4	observed	observe	VBN
cord-326027-58whwspe	328	5	by	by	IN
cord-326027-58whwspe	328	6	detection	detection	NN
cord-326027-58whwspe	328	7	of	of	IN
cord-326027-58whwspe	328	8	fluorescence	fluorescence	NN
cord-326027-58whwspe	328	9	at	at	IN
cord-326027-58whwspe	328	10	λ	λ	NN
cord-326027-58whwspe	328	11	=	=	SYM
cord-326027-58whwspe	328	12	509	509	CD
cord-326027-58whwspe	328	13	nm	nm	NN
cord-326027-58whwspe	328	14	.	.	.
cord-326027-58whwspe	329	1	Nuclei	nucleus	NNS
cord-326027-58whwspe	329	2	as	as	RB
cord-326027-58whwspe	329	3	well	well	RB
cord-326027-58whwspe	329	4	as	as	IN
cord-326027-58whwspe	329	5	DNA	dna	NN
cord-326027-58whwspe	329	6	in	in	IN
cord-326027-58whwspe	329	7	virus	virus	NN
cord-326027-58whwspe	329	8	factories	factory	NNS
cord-326027-58whwspe	329	9	were	be	VBD
cord-326027-58whwspe	329	10	detected	detect	VBN
cord-326027-58whwspe	329	11	by	by	IN
cord-326027-58whwspe	329	12	staining	stain	VBG
cord-326027-58whwspe	329	13	with	with	IN
cord-326027-58whwspe	329	14	Hoechst	Hoechst	NNP
cord-326027-58whwspe	329	15	33342	33342	CD
cord-326027-58whwspe	329	16	.	.	.
cord-326027-58whwspe	330	1	Finally	finally	RB
cord-326027-58whwspe	330	2	,	,	,
cord-326027-58whwspe	330	3	cells	cell	NNS
cord-326027-58whwspe	330	4	were	be	VBD
cord-326027-58whwspe	330	5	mounted	mount	VBN
cord-326027-58whwspe	330	6	onto	onto	IN
cord-326027-58whwspe	330	7	slides	slide	NNS
cord-326027-58whwspe	330	8	using	use	VBG
cord-326027-58whwspe	330	9	Fluorsave	Fluorsave	NNP
cord-326027-58whwspe	330	10	reagent	reagent	NN
cord-326027-58whwspe	330	11	(	(	-LRB-
cord-326027-58whwspe	330	12	Calbiochem	Calbiochem	NNP
cord-326027-58whwspe	330	13	)	)	-RRB-
cord-326027-58whwspe	330	14	.	.	.
cord-326027-58whwspe	331	1	Conventional	conventional	JJ
cord-326027-58whwspe	331	2	microscopy	microscopy	NNS
cord-326027-58whwspe	331	3	was	be	VBD
cord-326027-58whwspe	331	4	carried	carry	VBN
cord-326027-58whwspe	331	5	out	out	RP
cord-326027-58whwspe	331	6	in	in	IN
cord-326027-58whwspe	331	7	a	a	DT
cord-326027-58whwspe	331	8	Leica	Leica	NNP
cord-326027-58whwspe	331	9	photomicroscope	photomicroscope	NN
cord-326027-58whwspe	331	10	with	with	IN
cord-326027-58whwspe	331	11	a	a	DT
cord-326027-58whwspe	331	12	digital	digital	JJ
cord-326027-58whwspe	331	13	camera	camera	NN
cord-326027-58whwspe	331	14	,	,	,
cord-326027-58whwspe	331	15	and	and	CC
cord-326027-58whwspe	331	16	digitized	digitize	VBN
cord-326027-58whwspe	331	17	images	image	NNS
cord-326027-58whwspe	331	18	were	be	VBD
cord-326027-58whwspe	331	19	obtained	obtain	VBN
cord-326027-58whwspe	331	20	with	with	IN
cord-326027-58whwspe	331	21	Qwin	Qwin	NNP
cord-326027-58whwspe	331	22	software	software	NN
cord-326027-58whwspe	331	23	(	(	-LRB-
cord-326027-58whwspe	331	24	Leica	Leica	NNP
cord-326027-58whwspe	331	25	)	)	-RRB-
cord-326027-58whwspe	331	26	.	.	.
cord-326027-58whwspe	332	1	Confocal	Confocal	NNP
cord-326027-58whwspe	332	2	microscopy	microscopy	NNS
cord-326027-58whwspe	332	3	was	be	VBD
cord-326027-58whwspe	332	4	carried	carry	VBN
cord-326027-58whwspe	332	5	out	out	RP
cord-326027-58whwspe	332	6	in	in	IN
cord-326027-58whwspe	332	7	a	a	DT
cord-326027-58whwspe	332	8	Leica	Leica	NNP
cord-326027-58whwspe	332	9	confocal	confocal	JJ
cord-326027-58whwspe	332	10	microscope	microscope	NN
cord-326027-58whwspe	332	11	TCS	TCS	NNP
cord-326027-58whwspe	332	12	SP2-AOBS	SP2-AOBS	NNP
cord-326027-58whwspe	332	13	equipped	equip	VBN
cord-326027-58whwspe	332	14	with	with	IN
cord-326027-58whwspe	332	15	a	a	DT
cord-326027-58whwspe	332	16	63	63	CD
cord-326027-58whwspe	332	17	or	or	CC
cord-326027-58whwspe	332	18	100×	100×	CD
cord-326027-58whwspe	332	19	objectives	objective	NNS
cord-326027-58whwspe	332	20	.	.	.
cord-326027-58whwspe	333	1	Vero	Vero	NNP
cord-326027-58whwspe	333	2	cells	cell	NNS
cord-326027-58whwspe	333	3	were	be	VBD
cord-326027-58whwspe	333	4	plated	plate	VBN
cord-326027-58whwspe	333	5	at	at	IN
cord-326027-58whwspe	333	6	60	60	CD
cord-326027-58whwspe	333	7	%	%	NN
cord-326027-58whwspe	333	8	confluence	confluence	NN
cord-326027-58whwspe	333	9	onto	onto	IN
cord-326027-58whwspe	333	10	35	35	CD
cord-326027-58whwspe	333	11	mm	mm	NN
cord-326027-58whwspe	333	12	dishes	dish	NNS
cord-326027-58whwspe	333	13	(	(	-LRB-
cord-326027-58whwspe	333	14	WillCo	WillCo	NNP
cord-326027-58whwspe	333	15	Wells	Wells	NNP
cord-326027-58whwspe	333	16	)	)	-RRB-
cord-326027-58whwspe	333	17	and	and	CC
cord-326027-58whwspe	333	18	infected	infect	VBN
cord-326027-58whwspe	333	19	with	with	IN
cord-326027-58whwspe	333	20	1	1	CD
cord-326027-58whwspe	333	21	or	or	CC
cord-326027-58whwspe	333	22	20	20	CD
cord-326027-58whwspe	333	23	pfu	pfu	NN
cord-326027-58whwspe	333	24	of	of	IN
cord-326027-58whwspe	333	25	B54GFP-2	b54gfp-2	NN
cord-326027-58whwspe	333	26	per	per	IN
cord-326027-58whwspe	333	27	cell	cell	NN
cord-326027-58whwspe	333	28	,	,	,
cord-326027-58whwspe	333	29	depending	depend	VBG
cord-326027-58whwspe	333	30	on	on	IN
cord-326027-58whwspe	333	31	the	the	DT
cord-326027-58whwspe	333	32	experiment	experiment	NN
cord-326027-58whwspe	333	33	.	.	.
cord-326027-58whwspe	334	1	For	for	IN
cord-326027-58whwspe	334	2	the	the	DT
cord-326027-58whwspe	334	3	majority	majority	NN
cord-326027-58whwspe	334	4	of	of	IN
cord-326027-58whwspe	334	5	experiments	experiment	NNS
cord-326027-58whwspe	334	6	,	,	,
cord-326027-58whwspe	334	7	synchronization	synchronization	NN
cord-326027-58whwspe	334	8	of	of	IN
cord-326027-58whwspe	334	9	virus	virus	NN
cord-326027-58whwspe	334	10	infection	infection	NN
cord-326027-58whwspe	334	11	was	be	VBD
cord-326027-58whwspe	334	12	achieved	achieve	VBN
cord-326027-58whwspe	334	13	by	by	IN
cord-326027-58whwspe	334	14	performing	perform	VBG
cord-326027-58whwspe	334	15	the	the	DT
cord-326027-58whwspe	334	16	adsorption	adsorption	NN
cord-326027-58whwspe	334	17	at	at	IN
cord-326027-58whwspe	334	18	4	4	CD
cord-326027-58whwspe	334	19	°	°	NN
cord-326027-58whwspe	334	20	C	c	NN
cord-326027-58whwspe	334	21	for	for	IN
cord-326027-58whwspe	334	22	90	90	CD
cord-326027-58whwspe	334	23	min	min	NN
cord-326027-58whwspe	334	24	and	and	CC
cord-326027-58whwspe	334	25	following	follow	VBG
cord-326027-58whwspe	334	26	removal	removal	NN
cord-326027-58whwspe	334	27	of	of	IN
cord-326027-58whwspe	334	28	unbound	unbound	JJ
cord-326027-58whwspe	334	29	virus	virus	NN
cord-326027-58whwspe	334	30	.	.	.
cord-326027-58whwspe	335	1	At	at	IN
cord-326027-58whwspe	335	2	indicated	indicate	VBN
cord-326027-58whwspe	335	3	time	time	NN
cord-326027-58whwspe	335	4	points	point	NNS
cord-326027-58whwspe	335	5	,	,	,
cord-326027-58whwspe	335	6	cells	cell	NNS
cord-326027-58whwspe	335	7	were	be	VBD
cord-326027-58whwspe	335	8	imaged	image	VBN
cord-326027-58whwspe	335	9	by	by	IN
cord-326027-58whwspe	335	10	confocal	confocal	JJ
cord-326027-58whwspe	335	11	microscopy	microscopy	NNS
cord-326027-58whwspe	335	12	carried	carry	VBN
cord-326027-58whwspe	335	13	out	out	RP
cord-326027-58whwspe	335	14	in	in	IN
cord-326027-58whwspe	335	15	a	a	DT
cord-326027-58whwspe	335	16	Leica	Leica	NNP
cord-326027-58whwspe	335	17	confocal	confocal	JJ
cord-326027-58whwspe	335	18	microscope	microscope	NN
cord-326027-58whwspe	335	19	TCS	TCS	NNP
cord-326027-58whwspe	335	20	SP2-AOBS	SP2-AOBS	NNP
cord-326027-58whwspe	335	21	.	.	.
cord-326027-58whwspe	336	1	In	in	IN
cord-326027-58whwspe	336	2	either	either	DT
cord-326027-58whwspe	336	3	case	case	NN
cord-326027-58whwspe	336	4	,	,	,
cord-326027-58whwspe	336	5	cells	cell	NNS
cord-326027-58whwspe	336	6	were	be	VBD
cord-326027-58whwspe	336	7	maintained	maintain	VBN
cord-326027-58whwspe	336	8	on	on	IN
cord-326027-58whwspe	336	9	a	a	DT
cord-326027-58whwspe	336	10	heated	heated	JJ
cord-326027-58whwspe	336	11	35	35	CD
cord-326027-58whwspe	336	12	mm	mm	CD
cord-326027-58whwspe	336	13	stage	stage	NN
cord-326027-58whwspe	336	14	with	with	IN
cord-326027-58whwspe	336	15	the	the	DT
cord-326027-58whwspe	336	16	temperature	temperature	NN
cord-326027-58whwspe	336	17	set	set	VBN
cord-326027-58whwspe	336	18	at	at	IN
cord-326027-58whwspe	336	19	37	37	CD
cord-326027-58whwspe	336	20	°	°	NN
cord-326027-58whwspe	336	21	C	c	NN
cord-326027-58whwspe	336	22	and	and	CC
cord-326027-58whwspe	336	23	fresh	fresh	JJ
cord-326027-58whwspe	336	24	DMEM	DMEM	NNP
cord-326027-58whwspe	336	25	supplemented	supplement	VBD
cord-326027-58whwspe	336	26	with	with	IN
cord-326027-58whwspe	336	27	2.5	2.5	CD
cord-326027-58whwspe	336	28	%	%	NN
cord-326027-58whwspe	336	29	fetal	fetal	JJ
cord-326027-58whwspe	336	30	bovine	bovine	JJ
cord-326027-58whwspe	336	31	serum	serum	NN
cord-326027-58whwspe	336	32	was	be	VBD
cord-326027-58whwspe	336	33	perfused	perfuse	VBN
cord-326027-58whwspe	336	34	onto	onto	IN
cord-326027-58whwspe	336	35	the	the	DT
cord-326027-58whwspe	336	36	dish	dish	NN
cord-326027-58whwspe	336	37	at	at	IN
cord-326027-58whwspe	336	38	a	a	DT
cord-326027-58whwspe	336	39	rate	rate	NN
cord-326027-58whwspe	336	40	of	of	IN
cord-326027-58whwspe	336	41	0.1	0.1	CD
cord-326027-58whwspe	336	42	ml	ml	NNS
cord-326027-58whwspe	336	43	/	/	SYM
cord-326027-58whwspe	336	44	min	min	NN
cord-326027-58whwspe	336	45	throughout	throughout	IN
cord-326027-58whwspe	336	46	the	the	DT
cord-326027-58whwspe	336	47	experiment	experiment	NN
cord-326027-58whwspe	336	48	.	.	.
cord-326027-58whwspe	337	1	The	the	DT
cord-326027-58whwspe	337	2	acquisition	acquisition	NN
cord-326027-58whwspe	337	3	of	of	IN
cord-326027-58whwspe	337	4	images	image	NNS
cord-326027-58whwspe	337	5	was	be	VBD
cord-326027-58whwspe	337	6	every	every	DT
cord-326027-58whwspe	337	7	5	5	CD
cord-326027-58whwspe	337	8	min	min	NN
cord-326027-58whwspe	337	9	in	in	IN
cord-326027-58whwspe	337	10	long	long	JJ
cord-326027-58whwspe	337	11	experiments	experiment	NNS
cord-326027-58whwspe	337	12	(	(	-LRB-
cord-326027-58whwspe	337	13	4	4	CD
cord-326027-58whwspe	337	14	to	to	TO
cord-326027-58whwspe	337	15	8	8	CD
cord-326027-58whwspe	337	16	h	h	NN
cord-326027-58whwspe	337	17	length	length	NN
cord-326027-58whwspe	337	18	)	)	-RRB-
cord-326027-58whwspe	337	19	and	and	CC
cord-326027-58whwspe	337	20	every	every	DT
cord-326027-58whwspe	337	21	2	2	CD
cord-326027-58whwspe	337	22	-	-	SYM
cord-326027-58whwspe	337	23	3	3	CD
cord-326027-58whwspe	337	24	s	s	NN
cord-326027-58whwspe	337	25	in	in	IN
cord-326027-58whwspe	337	26	shorter	short	JJR
cord-326027-58whwspe	337	27	experiments	experiment	NNS
cord-326027-58whwspe	337	28	(	(	-LRB-
cord-326027-58whwspe	337	29	from	from	IN
cord-326027-58whwspe	337	30	1	1	CD
cord-326027-58whwspe	337	31	to	to	IN
cord-326027-58whwspe	337	32	4	4	CD
cord-326027-58whwspe	337	33	hpi	hpi	NNP
cord-326027-58whwspe	337	34	)	)	-RRB-
cord-326027-58whwspe	337	35	.	.	.
cord-326027-58whwspe	338	1	Nevertheless	nevertheless	RB
cord-326027-58whwspe	338	2	,	,	,
cord-326027-58whwspe	338	3	in	in	IN
cord-326027-58whwspe	338	4	long	long	JJ
cord-326027-58whwspe	338	5	experiments	experiment	NNS
cord-326027-58whwspe	338	6	only	only	RB
cord-326027-58whwspe	338	7	indicated	indicate	VBD
cord-326027-58whwspe	338	8	postinfection	postinfection	NN
cord-326027-58whwspe	338	9	times	time	NNS
cord-326027-58whwspe	338	10	images	image	NNS
cord-326027-58whwspe	338	11	are	be	VBP
cord-326027-58whwspe	338	12	shown	show	VBN
cord-326027-58whwspe	338	13	in	in	IN
cord-326027-58whwspe	338	14	figures	figure	NNS
cord-326027-58whwspe	338	15	.	.	.
cord-326027-58whwspe	339	1	Simultaneous	simultaneous	JJ
cord-326027-58whwspe	339	2	acquisition	acquisition	NN
cord-326027-58whwspe	339	3	of	of	IN
cord-326027-58whwspe	339	4	EGFP	EGFP	NNP
cord-326027-58whwspe	339	5	fluorescence	fluorescence	NN
cord-326027-58whwspe	339	6	emission	emission	NN
cord-326027-58whwspe	339	7	and	and	CC
cord-326027-58whwspe	339	8	transmitted	transmit	VBN
cord-326027-58whwspe	339	9	light	light	NN
cord-326027-58whwspe	339	10	was	be	VBD
cord-326027-58whwspe	339	11	performed	perform	VBN
cord-326027-58whwspe	339	12	.	.	.
cord-326027-58whwspe	340	1	Microtubules	Microtubules	NNP
cord-326027-58whwspe	340	2	depolymerization	depolymerization	NN
cord-326027-58whwspe	340	3	was	be	VBD
cord-326027-58whwspe	340	4	achieved	achieve	VBN
cord-326027-58whwspe	340	5	with	with	IN
cord-326027-58whwspe	340	6	10	10	CD
cord-326027-58whwspe	340	7	μM	μm	CD
cord-326027-58whwspe	340	8	nocodazole	nocodazole	NNP
cord-326027-58whwspe	340	9	(	(	-LRB-
cord-326027-58whwspe	340	10	Sigma	Sigma	NNP
cord-326027-58whwspe	340	11	)	)	-RRB-
cord-326027-58whwspe	340	12	2	2	CD
cord-326027-58whwspe	340	13	h	h	NN
cord-326027-58whwspe	340	14	before	before	IN
cord-326027-58whwspe	340	15	the	the	DT
cord-326027-58whwspe	340	16	infection	infection	NN
cord-326027-58whwspe	340	17	without	without	IN
cord-326027-58whwspe	340	18	removing	remove	VBG
cord-326027-58whwspe	340	19	it	-PRON-	PRP
cord-326027-58whwspe	340	20	during	during	IN
cord-326027-58whwspe	340	21	the	the	DT
cord-326027-58whwspe	340	22	acquisition	acquisition	NN
cord-326027-58whwspe	340	23	of	of	IN
cord-326027-58whwspe	340	24	images	image	NNS
cord-326027-58whwspe	340	25	.	.	.
cord-326027-58whwspe	341	1	Detection	detection	NN
cord-326027-58whwspe	341	2	of	of	IN
cord-326027-58whwspe	341	3	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	341	4	in	in	IN
cord-326027-58whwspe	341	5	live	live	JJ
cord-326027-58whwspe	341	6	cells	cell	NNS
cord-326027-58whwspe	341	7	was	be	VBD
cord-326027-58whwspe	341	8	carried	carry	VBN
cord-326027-58whwspe	341	9	out	out	RP
cord-326027-58whwspe	341	10	with	with	IN
cord-326027-58whwspe	341	11	MitoTracker	MitoTracker	NNP
cord-326027-58whwspe	341	12	Red	Red	NNP
cord-326027-58whwspe	341	13	CMXRos	CMXRos	NNP
cord-326027-58whwspe	341	14	(	(	-LRB-
cord-326027-58whwspe	341	15	Molecular	Molecular	NNP
cord-326027-58whwspe	341	16	Probes	Probes	NNPS
cord-326027-58whwspe	341	17	)	)	-RRB-
cord-326027-58whwspe	342	1	100	100	CD
cord-326027-58whwspe	342	2	nM	nm	NN
cord-326027-58whwspe	342	3	for	for	IN
cord-326027-58whwspe	342	4	30	30	CD
cord-326027-58whwspe	342	5	min	min	NN
cord-326027-58whwspe	342	6	after	after	IN
cord-326027-58whwspe	342	7	virus	virus	NN
cord-326027-58whwspe	342	8	infection	infection	NN
cord-326027-58whwspe	342	9	.	.	.
cord-326027-58whwspe	343	1	Golgi	Golgi	NNP
cord-326027-58whwspe	343	2	complex	complex	NN
cord-326027-58whwspe	343	3	was	be	VBD
cord-326027-58whwspe	343	4	detected	detect	VBN
cord-326027-58whwspe	343	5	in	in	IN
cord-326027-58whwspe	343	6	live	live	JJ
cord-326027-58whwspe	343	7	cells	cell	NNS
cord-326027-58whwspe	343	8	with	with	IN
cord-326027-58whwspe	343	9	5	5	CD
cord-326027-58whwspe	344	1	μM	μM	NNP
cord-326027-58whwspe	344	2	Bodipy	Bodipy	NNP
cord-326027-58whwspe	344	3	-	-	HYPH
cord-326027-58whwspe	344	4	TRC	TRC	NNP
cord-326027-58whwspe	345	1	5	5	LS
cord-326027-58whwspe	345	2	-ceramide	-ceramide	.
cord-326027-58whwspe	346	1	complexed	complexed	JJ
cord-326027-58whwspe	346	2	to	to	IN
cord-326027-58whwspe	346	3	BSA	BSA	NNP
cord-326027-58whwspe	346	4	(	(	-LRB-
cord-326027-58whwspe	346	5	Molecular	Molecular	NNP
cord-326027-58whwspe	346	6	Probes	Probes	NNPS
cord-326027-58whwspe	346	7	)	)	-RRB-
cord-326027-58whwspe	346	8	for	for	IN
cord-326027-58whwspe	346	9	30	30	CD
cord-326027-58whwspe	346	10	min	min	NN
cord-326027-58whwspe	346	11	before	before	IN
cord-326027-58whwspe	346	12	infection	infection	NN
cord-326027-58whwspe	346	13	,	,	,
cord-326027-58whwspe	346	14	and	and	CC
cord-326027-58whwspe	346	15	emission	emission	NN
cord-326027-58whwspe	346	16	detected	detect	VBN
cord-326027-58whwspe	346	17	at	at	IN
cord-326027-58whwspe	346	18	λ	λ	NN
cord-326027-58whwspe	346	19	=	=	SYM
cord-326027-58whwspe	346	20	617	617	CD
cord-326027-58whwspe	346	21	.	.	.
cord-326027-58whwspe	347	1	Manipulation	manipulation	NN
cord-326027-58whwspe	347	2	and	and	CC
cord-326027-58whwspe	347	3	subsequent	subsequent	JJ
cord-326027-58whwspe	347	4	analysis	analysis	NN
cord-326027-58whwspe	347	5	of	of	IN
cord-326027-58whwspe	347	6	acquired	acquire	VBN
cord-326027-58whwspe	347	7	images	image	NNS
cord-326027-58whwspe	347	8	were	be	VBD
cord-326027-58whwspe	347	9	carried	carry	VBN
cord-326027-58whwspe	347	10	out	out	RP
cord-326027-58whwspe	347	11	with	with	IN
cord-326027-58whwspe	347	12	Leica	Leica	NNP
cord-326027-58whwspe	347	13	Confocal	Confocal	NNP
cord-326027-58whwspe	347	14	Software	Software	NNP
cord-326027-58whwspe	347	15	v.2.0	v.2.0	.
cord-326027-58whwspe	347	16	.	.	.
cord-326027-58whwspe	348	1	African	african	JJ
cord-326027-58whwspe	348	2	swine	swine	NN
cord-326027-58whwspe	348	3	fever	fever	NN
cord-326027-58whwspe	348	4	virus	virus	NN
cord-326027-58whwspe	348	5	protein	protein	NN
cord-326027-58whwspe	348	6	p54	p54	NN
cord-326027-58whwspe	348	7	interacts	interact	VBZ
cord-326027-58whwspe	348	8	with	with	IN
cord-326027-58whwspe	348	9	the	the	DT
cord-326027-58whwspe	348	10	microtubular	microtubular	NNP
cord-326027-58whwspe	348	11	motor	motor	NN
cord-326027-58whwspe	348	12	complex	complex	NN
cord-326027-58whwspe	348	13	through	through	IN
cord-326027-58whwspe	348	14	direct	direct	JJ
cord-326027-58whwspe	348	15	binding	binding	NN
cord-326027-58whwspe	348	16	to	to	IN
cord-326027-58whwspe	348	17	light	light	JJ
cord-326027-58whwspe	348	18	-	-	HYPH
cord-326027-58whwspe	348	19	chain	chain	NN
cord-326027-58whwspe	348	20	dynein	dynein	NN
cord-326027-58whwspe	348	21	Assembly	Assembly	NNP
cord-326027-58whwspe	348	22	of	of	IN
cord-326027-58whwspe	348	23	African	african	JJ
cord-326027-58whwspe	348	24	swine	swine	NN
cord-326027-58whwspe	348	25	fever	fever	NN
cord-326027-58whwspe	348	26	virus	virus	NN
cord-326027-58whwspe	348	27	:	:	:
cord-326027-58whwspe	348	28	role	role	NN
cord-326027-58whwspe	348	29	of	of	IN
cord-326027-58whwspe	348	30	polyprotein	polyprotein	NN
cord-326027-58whwspe	349	1	pp220	pp220	JJ
cord-326027-58whwspe	349	2	African	african	JJ
cord-326027-58whwspe	349	3	swine	swine	NN
cord-326027-58whwspe	349	4	fever	fever	NN
cord-326027-58whwspe	349	5	virus	virus	NN
cord-326027-58whwspe	349	6	is	be	VBZ
cord-326027-58whwspe	349	7	enveloped	envelop	VBN
cord-326027-58whwspe	349	8	by	by	IN
cord-326027-58whwspe	349	9	a	a	DT
cord-326027-58whwspe	349	10	two	two	CD
cord-326027-58whwspe	349	11	-	-	HYPH
cord-326027-58whwspe	349	12	membraned	membraned	NN
cord-326027-58whwspe	349	13	collapsed	collapsed	JJ
cord-326027-58whwspe	349	14	cisterna	cisterna	NNP
cord-326027-58whwspe	349	15	derived	derive	VBN
cord-326027-58whwspe	349	16	from	from	IN
cord-326027-58whwspe	349	17	the	the	DT
cord-326027-58whwspe	349	18	endoplasmic	endoplasmic	NN
cord-326027-58whwspe	349	19	reticulum	reticulum	NN
cord-326027-58whwspe	349	20	African	african	JJ
cord-326027-58whwspe	349	21	swine	swine	NN
cord-326027-58whwspe	349	22	fever	fever	NN
cord-326027-58whwspe	349	23	virus	virus	NN
cord-326027-58whwspe	349	24	protease	protease	NN
cord-326027-58whwspe	349	25	,	,	,
cord-326027-58whwspe	349	26	a	a	DT
cord-326027-58whwspe	349	27	new	new	JJ
cord-326027-58whwspe	349	28	viral	viral	JJ
cord-326027-58whwspe	349	29	member	member	NN
cord-326027-58whwspe	349	30	of	of	IN
cord-326027-58whwspe	349	31	the	the	DT
cord-326027-58whwspe	349	32	SUMO-1-specific	sumo-1-specific	CD
cord-326027-58whwspe	349	33	protease	protease	NN
cord-326027-58whwspe	349	34	family	family	NN
cord-326027-58whwspe	349	35	Repression	repression	NN
cord-326027-58whwspe	349	36	of	of	IN
cord-326027-58whwspe	349	37	African	african	JJ
cord-326027-58whwspe	349	38	swine	swine	NN
cord-326027-58whwspe	349	39	fever	fever	NN
cord-326027-58whwspe	349	40	virus	virus	NN
cord-326027-58whwspe	349	41	polyprotein	polyprotein	NNP
cord-326027-58whwspe	349	42	pp220-encoding	pp220-encode	VBG
cord-326027-58whwspe	349	43	gene	gene	NN
cord-326027-58whwspe	349	44	leads	lead	VBZ
cord-326027-58whwspe	349	45	to	to	IN
cord-326027-58whwspe	349	46	the	the	DT
cord-326027-58whwspe	349	47	assembly	assembly	NN
cord-326027-58whwspe	349	48	of	of	IN
cord-326027-58whwspe	349	49	icosahedral	icosahedral	JJ
cord-326027-58whwspe	349	50	core	core	NN
cord-326027-58whwspe	349	51	-	-	HYPH
cord-326027-58whwspe	349	52	less	less	JJR
cord-326027-58whwspe	349	53	particles	particle	NNS
cord-326027-58whwspe	349	54	Electron	electron	NN
cord-326027-58whwspe	349	55	microscopic	microscopic	JJ
cord-326027-58whwspe	349	56	observation	observation	NN
cord-326027-58whwspe	349	57	of	of	IN
cord-326027-58whwspe	349	58	African	african	JJ
cord-326027-58whwspe	349	59	swine	swine	NN
cord-326027-58whwspe	349	60	fever	fever	NN
cord-326027-58whwspe	349	61	virus	virus	NN
cord-326027-58whwspe	349	62	development	development	NN
cord-326027-58whwspe	349	63	in	in	IN
cord-326027-58whwspe	349	64	Vero	Vero	NNP
cord-326027-58whwspe	349	65	cells	cell	NNS
cord-326027-58whwspe	349	66	Characterization	characterization	NN
cord-326027-58whwspe	349	67	of	of	IN
cord-326027-58whwspe	349	68	African	african	JJ
cord-326027-58whwspe	349	69	swine	swine	NN
cord-326027-58whwspe	349	70	fever	fever	NN
cord-326027-58whwspe	349	71	virion	virion	NN
cord-326027-58whwspe	349	72	proteins	protein	VBZ
cord-326027-58whwspe	349	73	j5R	j5R	NNS
cord-326027-58whwspe	349	74	and	and	CC
cord-326027-58whwspe	349	75	j13L	j13L	NNP
cord-326027-58whwspe	349	76	:	:	:
cord-326027-58whwspe	349	77	immunolocalization	immunolocalization	NN
cord-326027-58whwspe	349	78	in	in	IN
cord-326027-58whwspe	349	79	virus	virus	NN
cord-326027-58whwspe	349	80	particles	particle	NNS
cord-326027-58whwspe	349	81	and	and	CC
cord-326027-58whwspe	349	82	assembly	assembly	NN
cord-326027-58whwspe	349	83	sites	site	NNS
cord-326027-58whwspe	349	84	Design	design	NN
cord-326027-58whwspe	349	85	and	and	CC
cord-326027-58whwspe	349	86	construction	construction	NN
cord-326027-58whwspe	349	87	of	of	IN
cord-326027-58whwspe	349	88	African	african	JJ
cord-326027-58whwspe	349	89	swine	swine	NN
cord-326027-58whwspe	349	90	fever	fever	NN
cord-326027-58whwspe	349	91	virus	virus	NN
cord-326027-58whwspe	349	92	chimeras	chimera	NNS
cord-326027-58whwspe	349	93	incorporating	incorporate	VBG
cord-326027-58whwspe	349	94	foreign	foreign	JJ
cord-326027-58whwspe	349	95	viral	viral	JJ
cord-326027-58whwspe	349	96	epitopes	epitope	NNS
cord-326027-58whwspe	349	97	Purification	purification	NN
cord-326027-58whwspe	349	98	and	and	CC
cord-326027-58whwspe	349	99	properties	property	NNS
cord-326027-58whwspe	349	100	of	of	IN
cord-326027-58whwspe	349	101	African	african	JJ
cord-326027-58whwspe	349	102	swine	swine	NN
cord-326027-58whwspe	349	103	fever	fever	NN
cord-326027-58whwspe	349	104	virus	virus	NN
cord-326027-58whwspe	349	105	Vaccinia	vaccinia	NN
cord-326027-58whwspe	349	106	virus	virus	NN
cord-326027-58whwspe	349	107	cores	core	NNS
cord-326027-58whwspe	349	108	are	be	VBP
cord-326027-58whwspe	349	109	transported	transport	VBN
cord-326027-58whwspe	349	110	on	on	IN
cord-326027-58whwspe	349	111	microtubules	microtubule	NNS
cord-326027-58whwspe	349	112	Association	Association	NNP
cord-326027-58whwspe	349	113	of	of	IN
cord-326027-58whwspe	349	114	African	african	JJ
cord-326027-58whwspe	349	115	swine	swine	NN
cord-326027-58whwspe	349	116	fever	fever	NN
cord-326027-58whwspe	349	117	virus	virus	NN
cord-326027-58whwspe	349	118	with	with	IN
cord-326027-58whwspe	349	119	the	the	DT
cord-326027-58whwspe	349	120	cytoskeleton	cytoskeleton	NN
cord-326027-58whwspe	350	1	The	the	DT
cord-326027-58whwspe	350	2	major	major	JJ
cord-326027-58whwspe	350	3	structural	structural	JJ
cord-326027-58whwspe	350	4	protein	protein	NN
cord-326027-58whwspe	350	5	of	of	IN
cord-326027-58whwspe	350	6	African	african	JJ
cord-326027-58whwspe	350	7	swine	swine	NN
cord-326027-58whwspe	350	8	fever	fever	NN
cord-326027-58whwspe	350	9	virus	virus	NN
cord-326027-58whwspe	350	10	,	,	,
cord-326027-58whwspe	350	11	p73	p73	CD
cord-326027-58whwspe	350	12	,	,	,
cord-326027-58whwspe	350	13	is	be	VBZ
cord-326027-58whwspe	350	14	packaged	package	VBN
cord-326027-58whwspe	350	15	into	into	IN
cord-326027-58whwspe	350	16	large	large	JJ
cord-326027-58whwspe	350	17	structures	structure	NNS
cord-326027-58whwspe	350	18	,	,	,
cord-326027-58whwspe	350	19	indicative	indicative	JJ
cord-326027-58whwspe	350	20	of	of	IN
cord-326027-58whwspe	350	21	viral	viral	JJ
cord-326027-58whwspe	350	22	capsid	capsid	NN
cord-326027-58whwspe	350	23	or	or	CC
cord-326027-58whwspe	350	24	matrix	matrix	NN
cord-326027-58whwspe	350	25	precursors	precursor	NNS
cord-326027-58whwspe	350	26	,	,	,
cord-326027-58whwspe	350	27	on	on	IN
cord-326027-58whwspe	350	28	the	the	DT
cord-326027-58whwspe	350	29	endoplasmic	endoplasmic	NN
cord-326027-58whwspe	350	30	reticulum	reticulum	NN
cord-326027-58whwspe	350	31	African	african	JJ
cord-326027-58whwspe	350	32	swine	swine	NN
cord-326027-58whwspe	350	33	fever	fever	NN
cord-326027-58whwspe	350	34	virus	virus	NN
cord-326027-58whwspe	350	35	interaction	interaction	NN
cord-326027-58whwspe	350	36	with	with	IN
cord-326027-58whwspe	350	37	microtubules	microtubule	NNS
cord-326027-58whwspe	351	1	The	the	DT
cord-326027-58whwspe	351	2	preparation	preparation	NN
cord-326027-58whwspe	351	3	of	of	IN
cord-326027-58whwspe	351	4	orthopoxvirus	orthopoxvirus	NNP
cord-326027-58whwspe	351	5	DNA	DNA	NNP
cord-326027-58whwspe	351	6	Two	Two	NNP
cord-326027-58whwspe	351	7	-	-	HYPH
cord-326027-58whwspe	351	8	dimensional	dimensional	JJ
cord-326027-58whwspe	351	9	analysis	analysis	NN
cord-326027-58whwspe	351	10	of	of	IN
cord-326027-58whwspe	351	11	African	african	JJ
cord-326027-58whwspe	351	12	swine	swine	NN
cord-326027-58whwspe	351	13	fever	fever	NN
cord-326027-58whwspe	351	14	virus	virus	NN
cord-326027-58whwspe	351	15	proteins	protein	NNS
cord-326027-58whwspe	351	16	and	and	CC
cord-326027-58whwspe	351	17	proteins	protein	NNS
cord-326027-58whwspe	351	18	induced	induce	VBN
cord-326027-58whwspe	351	19	in	in	IN
cord-326027-58whwspe	351	20	infected	infected	JJ
cord-326027-58whwspe	351	21	cells	cell	NNS
cord-326027-58whwspe	351	22	Tracking	track	VBG
cord-326027-58whwspe	351	23	fluorescence	fluorescence	NN
cord-326027-58whwspe	351	24	-	-	HYPH
cord-326027-58whwspe	351	25	labeled	label	VBN
cord-326027-58whwspe	351	26	rabies	rabies	NN
cord-326027-58whwspe	351	27	virus	virus	NN
cord-326027-58whwspe	351	28	:	:	:
cord-326027-58whwspe	351	29	enhanced	enhance	VBN
cord-326027-58whwspe	351	30	green	green	JJ
cord-326027-58whwspe	351	31	fluorescent	fluorescent	NN
cord-326027-58whwspe	351	32	protein	protein	NN
cord-326027-58whwspe	351	33	-	-	HYPH
cord-326027-58whwspe	351	34	tagged	tag	VBN
cord-326027-58whwspe	351	35	phosphoprotein	phosphoprotein	NNP
cord-326027-58whwspe	351	36	P	P	NNP
cord-326027-58whwspe	351	37	supports	support	VBZ
cord-326027-58whwspe	351	38	virus	virus	NN
cord-326027-58whwspe	351	39	gene	gene	NN
cord-326027-58whwspe	351	40	expression	expression	NN
cord-326027-58whwspe	351	41	and	and	CC
cord-326027-58whwspe	351	42	formation	formation	NN
cord-326027-58whwspe	351	43	of	of	IN
cord-326027-58whwspe	351	44	infectious	infectious	JJ
cord-326027-58whwspe	351	45	particles	particle	NNS
cord-326027-58whwspe	351	46	Inducible	inducible	JJ
cord-326027-58whwspe	351	47	gene	gene	NN
cord-326027-58whwspe	351	48	expression	expression	NN
cord-326027-58whwspe	351	49	from	from	IN
cord-326027-58whwspe	351	50	African	african	JJ
cord-326027-58whwspe	351	51	swine	swine	NN
cord-326027-58whwspe	351	52	fever	fever	NN
cord-326027-58whwspe	351	53	virus	virus	NN
cord-326027-58whwspe	351	54	recombinants	recombinant	NNS
cord-326027-58whwspe	351	55	:	:	:
cord-326027-58whwspe	351	56	analysis	analysis	NN
cord-326027-58whwspe	351	57	of	of	IN
cord-326027-58whwspe	351	58	the	the	DT
cord-326027-58whwspe	351	59	major	major	JJ
cord-326027-58whwspe	351	60	capsid	capsid	NN
cord-326027-58whwspe	351	61	protein	protein	NN
cord-326027-58whwspe	351	62	p72	p72	NN
cord-326027-58whwspe	351	63	Movements	movement	NNS
cord-326027-58whwspe	351	64	of	of	IN
cord-326027-58whwspe	351	65	vaccinia	vaccinia	NN
cord-326027-58whwspe	351	66	virus	virus	NN
cord-326027-58whwspe	351	67	intracellular	intracellular	JJ
cord-326027-58whwspe	351	68	enveloped	envelop	VBD
cord-326027-58whwspe	351	69	virions	virion	NNS
cord-326027-58whwspe	351	70	with	with	IN
cord-326027-58whwspe	351	71	GFP	GFP	NNP
cord-326027-58whwspe	351	72	tagged	tagged	NNP
cord-326027-58whwspe	351	73	to	to	IN
cord-326027-58whwspe	351	74	the	the	DT
cord-326027-58whwspe	351	75	F13L	F13L	NNP
cord-326027-58whwspe	351	76	envelope	envelope	NN
cord-326027-58whwspe	351	77	protein	protein	NN
cord-326027-58whwspe	351	78	Microtubule	Microtubule	NNP
cord-326027-58whwspe	351	79	-	-	HYPH
cord-326027-58whwspe	351	80	independent	independent	JJ
cord-326027-58whwspe	351	81	motility	motility	NN
cord-326027-58whwspe	351	82	and	and	CC
cord-326027-58whwspe	351	83	nuclear	nuclear	JJ
cord-326027-58whwspe	351	84	targeting	targeting	NN
cord-326027-58whwspe	351	85	of	of	IN
cord-326027-58whwspe	351	86	adenoviruses	adenovirus	NNS
cord-326027-58whwspe	351	87	with	with	IN
cord-326027-58whwspe	351	88	fluorescently	fluorescently	RB
cord-326027-58whwspe	351	89	labeled	label	VBN
cord-326027-58whwspe	351	90	genomes	genome	NNS
cord-326027-58whwspe	351	91	Improvement	improvement	NN
cord-326027-58whwspe	351	92	of	of	IN
cord-326027-58whwspe	351	93	African	african	JJ
cord-326027-58whwspe	351	94	swine	swine	NN
cord-326027-58whwspe	351	95	fever	fever	NN
cord-326027-58whwspe	351	96	virus	virus	NN
cord-326027-58whwspe	351	97	neutralization	neutralization	NN
cord-326027-58whwspe	351	98	assay	assay	NN
cord-326027-58whwspe	351	99	using	use	VBG
cord-326027-58whwspe	351	100	recombinant	recombinant	JJ
cord-326027-58whwspe	351	101	viruses	virus	NNS
cord-326027-58whwspe	351	102	expressing	express	VBG
cord-326027-58whwspe	351	103	chromogenic	chromogenic	JJ
cord-326027-58whwspe	351	104	marker	marker	NN
cord-326027-58whwspe	351	105	genes	gene	NNS
cord-326027-58whwspe	351	106	Aggresomes	Aggresomes	NNP
cord-326027-58whwspe	351	107	resemble	resemble	VBP
cord-326027-58whwspe	351	108	sites	site	NNS
cord-326027-58whwspe	351	109	specialized	specialize	VBN
cord-326027-58whwspe	351	110	for	for	IN
cord-326027-58whwspe	351	111	virus	virus	NNP
cord-326027-58whwspe	351	112	assembly	assembly	NN
cord-326027-58whwspe	352	1	The	the	DT
cord-326027-58whwspe	352	2	African	african	JJ
cord-326027-58whwspe	352	3	swine	swine	NN
cord-326027-58whwspe	352	4	fever	fever	NN
cord-326027-58whwspe	352	5	virus	virus	NN
cord-326027-58whwspe	352	6	dynein	dynein	NN
cord-326027-58whwspe	352	7	-	-	HYPH
cord-326027-58whwspe	352	8	binding	bind	VBG
cord-326027-58whwspe	352	9	protein	protein	NN
cord-326027-58whwspe	352	10	p54	p54	NN
cord-326027-58whwspe	352	11	induces	induce	VBZ
cord-326027-58whwspe	352	12	infected	infected	JJ
cord-326027-58whwspe	352	13	cell	cell	NN
cord-326027-58whwspe	352	14	apoptosis	apoptosis	NN
cord-326027-58whwspe	352	15	Switching	switch	VBG
cord-326027-58whwspe	352	16	on	on	IN
cord-326027-58whwspe	352	17	and	and	CC
cord-326027-58whwspe	352	18	off	off	IN
cord-326027-58whwspe	352	19	the	the	DT
cord-326027-58whwspe	352	20	cell	cell	NN
cord-326027-58whwspe	352	21	death	death	NN
cord-326027-58whwspe	352	22	cascade	cascade	NN
cord-326027-58whwspe	352	23	:	:	:
cord-326027-58whwspe	353	1	African	african	JJ
cord-326027-58whwspe	353	2	swine	swine	NN
cord-326027-58whwspe	353	3	fever	fever	NN
cord-326027-58whwspe	353	4	virus	virus	NN
cord-326027-58whwspe	353	5	apoptosis	apoptosis	NN
cord-326027-58whwspe	353	6	regulation	regulation	NN
cord-326027-58whwspe	353	7	Vaccinia	Vaccinia	NNP
cord-326027-58whwspe	353	8	virus	virus	NN
cord-326027-58whwspe	353	9	utilizes	utilize	VBZ
cord-326027-58whwspe	353	10	microtubules	microtubule	NNS
cord-326027-58whwspe	353	11	for	for	IN
cord-326027-58whwspe	353	12	movement	movement	NN
cord-326027-58whwspe	353	13	to	to	IN
cord-326027-58whwspe	353	14	the	the	DT
cord-326027-58whwspe	353	15	cell	cell	NN
cord-326027-58whwspe	353	16	surface	surface	NN
cord-326027-58whwspe	354	1	The	the	DT
cord-326027-58whwspe	354	2	dynein	dynein	NN
cord-326027-58whwspe	354	3	microtubule	microtubule	NN
cord-326027-58whwspe	354	4	motor	motor	NN
cord-326027-58whwspe	354	5	Cytoarchitecture	Cytoarchitecture	NNP
cord-326027-58whwspe	354	6	and	and	CC
cord-326027-58whwspe	354	7	physical	physical	JJ
cord-326027-58whwspe	354	8	properties	property	NNS
cord-326027-58whwspe	354	9	of	of	IN
cord-326027-58whwspe	354	10	cytoplasm	cytoplasm	NN
cord-326027-58whwspe	354	11	:	:	:
cord-326027-58whwspe	355	1	volume	volume	NN
cord-326027-58whwspe	355	2	,	,	,
cord-326027-58whwspe	355	3	viscosity	viscosity	NN
cord-326027-58whwspe	355	4	,	,	,
cord-326027-58whwspe	355	5	diffusion	diffusion	NN
cord-326027-58whwspe	355	6	,	,	,
cord-326027-58whwspe	355	7	intracellular	intracellular	JJ
cord-326027-58whwspe	355	8	surface	surface	NN
cord-326027-58whwspe	355	9	area	area	NN
cord-326027-58whwspe	355	10	Green	green	JJ
cord-326027-58whwspe	355	11	fluorescent	fluorescent	NN
cord-326027-58whwspe	355	12	protein	protein	NN
cord-326027-58whwspe	355	13	-	-	HYPH
cord-326027-58whwspe	355	14	tagged	tag	VBN
cord-326027-58whwspe	355	15	adeno	adeno	NNP
cord-326027-58whwspe	355	16	-	-	HYPH
cord-326027-58whwspe	355	17	associated	associate	VBN
cord-326027-58whwspe	355	18	virus	virus	NN
cord-326027-58whwspe	355	19	particles	particle	NNS
cord-326027-58whwspe	355	20	allow	allow	VBP
cord-326027-58whwspe	355	21	the	the	DT
cord-326027-58whwspe	355	22	study	study	NN
cord-326027-58whwspe	355	23	of	of	IN
cord-326027-58whwspe	355	24	cytosolic	cytosolic	JJ
cord-326027-58whwspe	355	25	and	and	CC
cord-326027-58whwspe	355	26	nuclear	nuclear	JJ
cord-326027-58whwspe	355	27	trafficking	trafficking	NN
cord-326027-58whwspe	355	28	Visualization	visualization	NN
cord-326027-58whwspe	355	29	of	of	IN
cord-326027-58whwspe	355	30	the	the	DT
cord-326027-58whwspe	355	31	intracellular	intracellular	JJ
cord-326027-58whwspe	355	32	behavior	behavior	NN
cord-326027-58whwspe	355	33	of	of	IN
cord-326027-58whwspe	355	34	HIV	HIV	NNP
cord-326027-58whwspe	355	35	in	in	IN
cord-326027-58whwspe	355	36	living	living	NN
cord-326027-58whwspe	355	37	cells	cell	NNS
cord-326027-58whwspe	355	38	Construction	construction	NN
cord-326027-58whwspe	355	39	and	and	CC
cord-326027-58whwspe	355	40	characterization	characterization	NN
cord-326027-58whwspe	355	41	of	of	IN
cord-326027-58whwspe	355	42	a	a	DT
cord-326027-58whwspe	355	43	fluorescently	fluorescently	RB
cord-326027-58whwspe	355	44	labeled	label	VBN
cord-326027-58whwspe	355	45	infectious	infectious	JJ
cord-326027-58whwspe	355	46	human	human	JJ
cord-326027-58whwspe	355	47	immunodeficiency	immunodeficiency	NN
cord-326027-58whwspe	355	48	virus	virus	NN
cord-326027-58whwspe	355	49	type	type	NN
cord-326027-58whwspe	355	50	1	1	CD
cord-326027-58whwspe	355	51	derivative	derivative	JJ
cord-326027-58whwspe	355	52	Mitochondrial	mitochondrial	JJ
cord-326027-58whwspe	355	53	distribution	distribution	NN
cord-326027-58whwspe	355	54	and	and	CC
cord-326027-58whwspe	355	55	function	function	NN
cord-326027-58whwspe	355	56	in	in	IN
cord-326027-58whwspe	355	57	herpes	herpes	NN
cord-326027-58whwspe	355	58	simplex	simplex	NNP
cord-326027-58whwspe	355	59	virus	virus	NN
cord-326027-58whwspe	355	60	-	-	HYPH
cord-326027-58whwspe	355	61	infected	infect	VBN
cord-326027-58whwspe	355	62	cells	cell	NNS
cord-326027-58whwspe	355	63	Virus	virus	NN
cord-326027-58whwspe	355	64	factories	factory	NNS
cord-326027-58whwspe	355	65	:	:	:
cord-326027-58whwspe	355	66	associations	association	NNS
cord-326027-58whwspe	355	67	of	of	IN
cord-326027-58whwspe	355	68	cell	cell	NN
cord-326027-58whwspe	355	69	organelles	organelle	NNS
cord-326027-58whwspe	355	70	for	for	IN
cord-326027-58whwspe	355	71	viral	viral	JJ
cord-326027-58whwspe	355	72	replication	replication	NN
cord-326027-58whwspe	355	73	and	and	CC
cord-326027-58whwspe	355	74	morphogenesis	morphogenesis	NN
cord-326027-58whwspe	355	75	Ultrastructural	Ultrastructural	NNP
cord-326027-58whwspe	355	76	study	study	NN
cord-326027-58whwspe	355	77	of	of	IN
cord-326027-58whwspe	355	78	African	african	JJ
cord-326027-58whwspe	355	79	swine	swine	NN
cord-326027-58whwspe	355	80	fever	fever	NN
cord-326027-58whwspe	355	81	virus	virus	NN
cord-326027-58whwspe	355	82	replication	replication	NN
cord-326027-58whwspe	355	83	in	in	IN
cord-326027-58whwspe	355	84	cultures	culture	NNS
cord-326027-58whwspe	355	85	of	of	IN
cord-326027-58whwspe	355	86	swine	swine	NN
cord-326027-58whwspe	355	87	bone	bone	NN
cord-326027-58whwspe	355	88	marrow	marrow	NN
cord-326027-58whwspe	355	89	cells	cell	NNS
cord-326027-58whwspe	356	1	Apoptosis	apoptosis	NN
cord-326027-58whwspe	356	2	:	:	:
cord-326027-58whwspe	356	3	a	a	DT
cord-326027-58whwspe	356	4	mechanism	mechanism	NN
cord-326027-58whwspe	356	5	of	of	IN
cord-326027-58whwspe	356	6	cell	cell	NN
cord-326027-58whwspe	356	7	killing	killing	NN
cord-326027-58whwspe	356	8	and	and	CC
cord-326027-58whwspe	356	9	lymphoid	lymphoid	JJ
cord-326027-58whwspe	356	10	organ	organ	NN
cord-326027-58whwspe	356	11	impairment	impairment	NN
cord-326027-58whwspe	356	12	during	during	IN
cord-326027-58whwspe	356	13	acute	acute	JJ
cord-326027-58whwspe	356	14	African	african	JJ
cord-326027-58whwspe	356	15	swine	swine	NN
cord-326027-58whwspe	356	16	fever	fever	NN
cord-326027-58whwspe	356	17	virus	virus	NN
cord-326027-58whwspe	356	18	infection	infection	NN
cord-326027-58whwspe	356	19	Interaction	Interaction	NNP
cord-326027-58whwspe	356	20	of	of	IN
cord-326027-58whwspe	356	21	the	the	DT
cord-326027-58whwspe	356	22	rabies	rabies	NN
cord-326027-58whwspe	356	23	virus	virus	NN
cord-326027-58whwspe	356	24	P	p	NN
cord-326027-58whwspe	356	25	protein	protein	NN
cord-326027-58whwspe	356	26	with	with	IN
cord-326027-58whwspe	356	27	the	the	DT
cord-326027-58whwspe	356	28	LC8	lc8	JJ
cord-326027-58whwspe	356	29	dynein	dynein	NNP
cord-326027-58whwspe	356	30	light	light	NN
cord-326027-58whwspe	356	31	chain	chain	NN
cord-326027-58whwspe	356	32	Genetic	genetic	JJ
cord-326027-58whwspe	356	33	manipulation	manipulation	NN
cord-326027-58whwspe	356	34	of	of	IN
cord-326027-58whwspe	356	35	African	african	JJ
cord-326027-58whwspe	356	36	swine	swine	NN
cord-326027-58whwspe	356	37	fever	fever	NN
cord-326027-58whwspe	356	38	virus	virus	NN
cord-326027-58whwspe	356	39	:	:	:
cord-326027-58whwspe	356	40	construction	construction	NN
cord-326027-58whwspe	356	41	of	of	IN
cord-326027-58whwspe	356	42	recombinant	recombinant	JJ
cord-326027-58whwspe	356	43	viruses	virus	NNS
cord-326027-58whwspe	356	44	expressing	express	VBG
cord-326027-58whwspe	356	45	the	the	DT
cord-326027-58whwspe	356	46	beta	beta	JJ
cord-326027-58whwspe	356	47	-	-	HYPH
cord-326027-58whwspe	356	48	galactosidase	galactosidase	NN
cord-326027-58whwspe	356	49	gene	gene	NN
cord-326027-58whwspe	356	50	Characterization	characterization	NN
cord-326027-58whwspe	356	51	and	and	CC
cord-326027-58whwspe	356	52	molecular	molecular	JJ
cord-326027-58whwspe	356	53	basis	basis	NN
cord-326027-58whwspe	356	54	of	of	IN
cord-326027-58whwspe	356	55	heterogeneity	heterogeneity	NN
cord-326027-58whwspe	356	56	of	of	IN
cord-326027-58whwspe	356	57	the	the	DT
cord-326027-58whwspe	356	58	African	african	JJ
cord-326027-58whwspe	356	59	swine	swine	NN
cord-326027-58whwspe	356	60	fever	fever	NN
cord-326027-58whwspe	356	61	virus	virus	NN
cord-326027-58whwspe	356	62	envelope	envelope	NN
cord-326027-58whwspe	356	63	protein	protein	NN
cord-326027-58whwspe	356	64	p54	p54	NN
cord-326027-58whwspe	357	1	The	the	DT
cord-326027-58whwspe	357	2	structural	structural	JJ
cord-326027-58whwspe	357	3	protein	protein	NN
cord-326027-58whwspe	357	4	p54	p54	NN
cord-326027-58whwspe	357	5	is	be	VBZ
cord-326027-58whwspe	357	6	essential	essential	JJ
cord-326027-58whwspe	357	7	for	for	IN
cord-326027-58whwspe	357	8	African	african	JJ
cord-326027-58whwspe	357	9	swine	swine	NN
cord-326027-58whwspe	357	10	fever	fever	NN
cord-326027-58whwspe	357	11	virus	virus	NN
cord-326027-58whwspe	357	12	viability	viability	NN
cord-326027-58whwspe	357	13	African	african	JJ
cord-326027-58whwspe	357	14	swine	swine	NN
cord-326027-58whwspe	357	15	fever	fever	NN
cord-326027-58whwspe	357	16	virus	virus	NN
cord-326027-58whwspe	357	17	structural	structural	JJ
cord-326027-58whwspe	357	18	protein	protein	NN
cord-326027-58whwspe	357	19	p54	p54	NN
cord-326027-58whwspe	357	20	is	be	VBZ
cord-326027-58whwspe	357	21	essential	essential	JJ
cord-326027-58whwspe	357	22	for	for	IN
cord-326027-58whwspe	357	23	the	the	DT
cord-326027-58whwspe	357	24	recruitment	recruitment	NN
cord-326027-58whwspe	357	25	of	of	IN
cord-326027-58whwspe	357	26	envelope	envelope	NN
cord-326027-58whwspe	357	27	precursors	precursor	NNS
cord-326027-58whwspe	357	28	to	to	IN
cord-326027-58whwspe	357	29	assembly	assembly	NN
cord-326027-58whwspe	357	30	sites	site	NNS
cord-326027-58whwspe	357	31	Migration	migration	NN
cord-326027-58whwspe	357	32	of	of	IN
cord-326027-58whwspe	357	33	mitochondria	mitochondrion	NNS
cord-326027-58whwspe	357	34	to	to	IN
cord-326027-58whwspe	357	35	viral	viral	JJ
cord-326027-58whwspe	357	36	assembly	assembly	NN
cord-326027-58whwspe	357	37	sites	site	NNS
cord-326027-58whwspe	357	38	in	in	IN
cord-326027-58whwspe	357	39	African	african	JJ
cord-326027-58whwspe	357	40	swine	swine	NN
cord-326027-58whwspe	357	41	fever	fever	NN
cord-326027-58whwspe	357	42	virus	virus	NN
cord-326027-58whwspe	357	43	-	-	HYPH
cord-326027-58whwspe	357	44	infected	infect	VBN
cord-326027-58whwspe	357	45	cells	cell	NNS
cord-326027-58whwspe	358	1	African	african	JJ
cord-326027-58whwspe	358	2	swine	swine	NN
cord-326027-58whwspe	358	3	fever	fever	NN
cord-326027-58whwspe	358	4	virus	virus	NN
cord-326027-58whwspe	358	5	is	be	VBZ
cord-326027-58whwspe	358	6	wrapped	wrap	VBN
cord-326027-58whwspe	358	7	by	by	IN
cord-326027-58whwspe	358	8	the	the	DT
cord-326027-58whwspe	358	9	endoplasmic	endoplasmic	NN
cord-326027-58whwspe	358	10	reticulum	reticulum	NN
cord-326027-58whwspe	358	11	Human	Human	NNP
cord-326027-58whwspe	358	12	cytomegalovirus	cytomegalovirus	NN
cord-326027-58whwspe	358	13	labeled	label	VBN
cord-326027-58whwspe	358	14	with	with	IN
cord-326027-58whwspe	358	15	green	green	JJ
cord-326027-58whwspe	358	16	fluorescent	fluorescent	NN
cord-326027-58whwspe	358	17	protein	protein	NN
cord-326027-58whwspe	358	18	for	for	IN
cord-326027-58whwspe	358	19	live	live	JJ
cord-326027-58whwspe	358	20	analysis	analysis	NN
cord-326027-58whwspe	358	21	of	of	IN
cord-326027-58whwspe	358	22	intracellular	intracellular	JJ
cord-326027-58whwspe	358	23	particle	particle	NN
cord-326027-58whwspe	358	24	movements	movement	NNS
cord-326027-58whwspe	358	25	Break	Break	NNP
cord-326027-58whwspe	358	26	ins	in	NNS
cord-326027-58whwspe	358	27	and	and	CC
cord-326027-58whwspe	358	28	break	break	VB
cord-326027-58whwspe	358	29	outs	out	NNS
cord-326027-58whwspe	358	30	:	:	:
cord-326027-58whwspe	358	31	viral	viral	JJ
cord-326027-58whwspe	358	32	interactions	interaction	NNS
cord-326027-58whwspe	358	33	with	with	IN
cord-326027-58whwspe	358	34	the	the	DT
cord-326027-58whwspe	358	35	cytoskeleton	cytoskeleton	NN
cord-326027-58whwspe	358	36	of	of	IN
cord-326027-58whwspe	358	37	mammalian	mammalian	JJ
cord-326027-58whwspe	358	38	cells	cell	NNS
cord-326027-58whwspe	358	39	Microtubule	Microtubule	NNP
cord-326027-58whwspe	358	40	-	-	HYPH
cord-326027-58whwspe	358	41	mediated	mediate	VBN
cord-326027-58whwspe	358	42	transport	transport	NN
cord-326027-58whwspe	358	43	of	of	IN
cord-326027-58whwspe	358	44	incoming	incoming	JJ
cord-326027-58whwspe	358	45	herpes	herpes	NNP
cord-326027-58whwspe	358	46	simplex	simplex	NNP
cord-326027-58whwspe	358	47	virus	virus	NN
cord-326027-58whwspe	358	48	1	1	CD
cord-326027-58whwspe	358	49	capsids	capsid	NNS
cord-326027-58whwspe	358	50	to	to	IN
cord-326027-58whwspe	358	51	the	the	DT
cord-326027-58whwspe	358	52	nucleus	nucleus	NN
cord-326027-58whwspe	358	53	Vimentin	Vimentin	NNP
cord-326027-58whwspe	358	54	rearrangement	rearrangement	NN
cord-326027-58whwspe	358	55	during	during	IN
cord-326027-58whwspe	358	56	African	african	JJ
cord-326027-58whwspe	358	57	swine	swine	NN
cord-326027-58whwspe	358	58	fever	fever	NN
cord-326027-58whwspe	358	59	virus	virus	NN
cord-326027-58whwspe	358	60	infection	infection	NN
cord-326027-58whwspe	358	61	involves	involve	VBZ
cord-326027-58whwspe	358	62	retrograde	retrograde	JJ
cord-326027-58whwspe	358	63	transport	transport	NN
cord-326027-58whwspe	358	64	along	along	IN
cord-326027-58whwspe	358	65	microtubules	microtubule	NNS
cord-326027-58whwspe	358	66	and	and	CC
cord-326027-58whwspe	358	67	phosphorylation	phosphorylation	NN
cord-326027-58whwspe	358	68	of	of	IN
cord-326027-58whwspe	358	69	vimentin	vimentin	NNS
cord-326027-58whwspe	358	70	by	by	IN
cord-326027-58whwspe	358	71	calcium	calcium	NN
cord-326027-58whwspe	358	72	calmodulin	calmodulin	NN
cord-326027-58whwspe	358	73	kinase	kinase	VBZ
cord-326027-58whwspe	358	74	II	II	NNP
cord-326027-58whwspe	358	75	Microtubule	Microtubule	NNP
cord-326027-58whwspe	358	76	-	-	HYPH
cord-326027-58whwspe	358	77	dependent	dependent	JJ
cord-326027-58whwspe	358	78	plus	plus	NN
cord-326027-58whwspe	358	79	-	-	HYPH
cord-326027-58whwspe	358	80	and	and	CC
cord-326027-58whwspe	358	81	minus	minus	CC
cord-326027-58whwspe	358	82	end	end	NN
cord-326027-58whwspe	358	83	-	-	HYPH
cord-326027-58whwspe	358	84	directed	direct	VBN
cord-326027-58whwspe	358	85	motilities	motility	NNS
cord-326027-58whwspe	358	86	are	be	VBP
cord-326027-58whwspe	358	87	competing	compete	VBG
cord-326027-58whwspe	358	88	processes	process	NNS
cord-326027-58whwspe	358	89	for	for	IN
cord-326027-58whwspe	358	90	nuclear	nuclear	JJ
cord-326027-58whwspe	358	91	targeting	targeting	NN
cord-326027-58whwspe	358	92	of	of	IN
cord-326027-58whwspe	358	93	adenovirus	adenovirus	NN
cord-326027-58whwspe	358	94	Visualization	visualization	NN
cord-326027-58whwspe	358	95	and	and	CC
cord-326027-58whwspe	358	96	characterization	characterization	NN
cord-326027-58whwspe	358	97	of	of	IN
cord-326027-58whwspe	358	98	the	the	DT
cord-326027-58whwspe	358	99	intracellular	intracellular	JJ
cord-326027-58whwspe	358	100	movement	movement	NN
cord-326027-58whwspe	358	101	of	of	IN
cord-326027-58whwspe	358	102	vaccinia	vaccinia	NN
cord-326027-58whwspe	358	103	virus	virus	NN
cord-326027-58whwspe	358	104	intracellular	intracellular	JJ
cord-326027-58whwspe	358	105	mature	mature	JJ
cord-326027-58whwspe	358	106	virions	virion	NNS
cord-326027-58whwspe	358	107	Visualization	visualization	NN
cord-326027-58whwspe	358	108	of	of	IN
cord-326027-58whwspe	358	109	intracellular	intracellular	JJ
cord-326027-58whwspe	358	110	movement	movement	NN
cord-326027-58whwspe	358	111	of	of	IN
cord-326027-58whwspe	358	112	vaccinia	vaccinia	NN
cord-326027-58whwspe	358	113	virus	virus	NN
cord-326027-58whwspe	358	114	virions	virion	NNS
cord-326027-58whwspe	358	115	containing	contain	VBG
cord-326027-58whwspe	358	116	a	a	DT
cord-326027-58whwspe	358	117	green	green	JJ
cord-326027-58whwspe	358	118	fluorescent	fluorescent	NN
cord-326027-58whwspe	358	119	protein	protein	NN
cord-326027-58whwspe	358	120	-	-	HYPH
cord-326027-58whwspe	358	121	B5R	b5r	CD
cord-326027-58whwspe	358	122	membrane	membrane	NN
cord-326027-58whwspe	358	123	protein	protein	NN
cord-326027-58whwspe	358	124	chimera	chimera	NN
cord-326027-58whwspe	359	1	This	this	DT
cord-326027-58whwspe	359	2	study	study	NN
cord-326027-58whwspe	359	3	was	be	VBD
cord-326027-58whwspe	359	4	supported	support	VBN
cord-326027-58whwspe	359	5	by	by	IN
cord-326027-58whwspe	359	6	grants	grant	NNS
cord-326027-58whwspe	359	7	from	from	IN
cord-326027-58whwspe	359	8	the	the	DT
cord-326027-58whwspe	359	9	Spanish	Spanish	NNP
cord-326027-58whwspe	359	10	Comisión	Comisión	NNP
cord-326027-58whwspe	359	11	Interministerial	Interministerial	NNP
cord-326027-58whwspe	359	12	de	de	FW
cord-326027-58whwspe	359	13	Ciencia	Ciencia	NNP
cord-326027-58whwspe	359	14	y	y	NNP
cord-326027-58whwspe	359	15	Tecnología	Tecnología	NNP
cord-326027-58whwspe	359	16	projects	project	NNS
cord-326027-58whwspe	360	1	AGL2002	AGL2002	NNP
cord-326027-58whwspe	360	2	-	-	HYPH
cord-326027-58whwspe	360	3	00668	00668	CD
cord-326027-58whwspe	360	4	,	,	,
cord-326027-58whwspe	360	5	AGL2004	agl2004	NN
cord-326027-58whwspe	360	6	-	-	HYPH
cord-326027-58whwspe	360	7	07857-C03	07857-c03	CD
cord-326027-58whwspe	360	8	-	-	HYPH
cord-326027-58whwspe	360	9	03	03	CD
cord-326027-58whwspe	360	10	,	,	,
cord-326027-58whwspe	360	11	BIO2004	BIO2004	NNP
cord-326027-58whwspe	360	12	-	-	HYPH
cord-326027-58whwspe	360	13	00690	00690	CD
cord-326027-58whwspe	360	14	and	and	CC
cord-326027-58whwspe	360	15	BIO2005	bio2005	NN
cord-326027-58whwspe	360	16	-	-	HYPH
cord-326027-58whwspe	360	17	0651	0651	CD
cord-326027-58whwspe	360	18	.	.	.
cord-326027-58whwspe	361	1	Supplementary	supplementary	JJ
cord-326027-58whwspe	361	2	data	datum	NNS
cord-326027-58whwspe	361	3	associated	associate	VBN
cord-326027-58whwspe	361	4	with	with	IN
cord-326027-58whwspe	361	5	this	this	DT
cord-326027-58whwspe	361	6	article	article	NN
cord-326027-58whwspe	361	7	can	can	MD
cord-326027-58whwspe	361	8	be	be	VB
cord-326027-58whwspe	361	9	found	find	VBN
cord-326027-58whwspe	361	10	in	in	IN
cord-326027-58whwspe	361	11	the	the	DT
cord-326027-58whwspe	361	12	online	online	JJ
cord-326027-58whwspe	361	13	version	version	NN
cord-326027-58whwspe	361	14	at	at	IN
cord-326027-58whwspe	361	15	doi:10.1016	doi:10.1016	NNP
cord-326027-58whwspe	361	16	/	/	SYM
cord-326027-58whwspe	361	17	j.virol.2006.01.021	j.virol.2006.01.021	XX
cord-326027-58whwspe	361	18	.	.	.