id sid tid token lemma pos cord-329245-6tj2k1yn 1 1 key key NN cord-329245-6tj2k1yn 1 2 : : : cord-329245-6tj2k1yn 2 1 cord-329245 cord-329245 NNP cord-329245-6tj2k1yn 2 2 - - HYPH cord-329245-6tj2k1yn 2 3 6tj2k1yn 6tj2k1yn CD cord-329245-6tj2k1yn 2 4 authors author NNS cord-329245-6tj2k1yn 2 5 : : : cord-329245-6tj2k1yn 2 6 Corse Corse NNP cord-329245-6tj2k1yn 2 7 , , , cord-329245-6tj2k1yn 2 8 Emily Emily NNP cord-329245-6tj2k1yn 2 9 ; ; : cord-329245-6tj2k1yn 2 10 Machamer Machamer NNP cord-329245-6tj2k1yn 2 11 , , , cord-329245-6tj2k1yn 2 12 Carolyn Carolyn NNP cord-329245-6tj2k1yn 2 13 E E NNP cord-329245-6tj2k1yn 2 14 title title NN cord-329245-6tj2k1yn 2 15 : : : cord-329245-6tj2k1yn 2 16 The the DT cord-329245-6tj2k1yn 2 17 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 2 18 tails tail NNS cord-329245-6tj2k1yn 2 19 of of IN cord-329245-6tj2k1yn 2 20 infectious infectious JJ cord-329245-6tj2k1yn 2 21 bronchitis bronchitis NN cord-329245-6tj2k1yn 2 22 virus virus NN cord-329245-6tj2k1yn 2 23 E e NN cord-329245-6tj2k1yn 2 24 and and CC cord-329245-6tj2k1yn 2 25 M M NNP cord-329245-6tj2k1yn 2 26 proteins protein NNS cord-329245-6tj2k1yn 2 27 mediate mediate VBP cord-329245-6tj2k1yn 2 28 their -PRON- PRP$ cord-329245-6tj2k1yn 2 29 interaction interaction NN cord-329245-6tj2k1yn 2 30 date date NN cord-329245-6tj2k1yn 2 31 : : : cord-329245-6tj2k1yn 2 32 2003 2003 CD cord-329245-6tj2k1yn 2 33 - - SYM cord-329245-6tj2k1yn 2 34 07 07 CD cord-329245-6tj2k1yn 2 35 - - HYPH cord-329245-6tj2k1yn 2 36 20 20 CD cord-329245-6tj2k1yn 2 37 journal journal NN cord-329245-6tj2k1yn 2 38 : : : cord-329245-6tj2k1yn 3 1 Virology virology NN cord-329245-6tj2k1yn 3 2 DOI DOI NNP cord-329245-6tj2k1yn 3 3 : : : cord-329245-6tj2k1yn 3 4 10.1016 10.1016 CD cord-329245-6tj2k1yn 3 5 / / SYM cord-329245-6tj2k1yn 3 6 s0042 s0042 NNP cord-329245-6tj2k1yn 3 7 - - HYPH cord-329245-6tj2k1yn 3 8 6822(03)00175 6822(03)00175 NNP cord-329245-6tj2k1yn 3 9 - - HYPH cord-329245-6tj2k1yn 3 10 2 2 CD cord-329245-6tj2k1yn 4 1 sha sha NNP cord-329245-6tj2k1yn 4 2 : : : cord-329245-6tj2k1yn 4 3 bf0d2c127636597ba7161c2fb21f1c9f8e06b091 bf0d2c127636597ba7161c2fb21f1c9f8e06b091 NNP cord-329245-6tj2k1yn 4 4 doc_id doc_id NNPS cord-329245-6tj2k1yn 4 5 : : : cord-329245-6tj2k1yn 4 6 329245 329245 CD cord-329245-6tj2k1yn 4 7 cord_uid cord_uid NNS cord-329245-6tj2k1yn 4 8 : : : cord-329245-6tj2k1yn 5 1 6tj2k1yn 6tj2k1yn CD cord-329245-6tj2k1yn 6 1 Abstract Abstract NNP cord-329245-6tj2k1yn 6 2 Virus Virus NNP cord-329245-6tj2k1yn 6 3 - - HYPH cord-329245-6tj2k1yn 6 4 like like JJ cord-329245-6tj2k1yn 6 5 particle particle NN cord-329245-6tj2k1yn 6 6 ( ( -LRB- cord-329245-6tj2k1yn 6 7 VLP VLP NNP cord-329245-6tj2k1yn 6 8 ) ) -RRB- cord-329245-6tj2k1yn 6 9 formation formation NN cord-329245-6tj2k1yn 6 10 by by IN cord-329245-6tj2k1yn 6 11 the the DT cord-329245-6tj2k1yn 6 12 coronavirus coronavirus NN cord-329245-6tj2k1yn 6 13 E e NN cord-329245-6tj2k1yn 6 14 and and CC cord-329245-6tj2k1yn 6 15 M M NNP cord-329245-6tj2k1yn 6 16 proteins protein NNS cord-329245-6tj2k1yn 6 17 suggests suggest VBZ cord-329245-6tj2k1yn 6 18 that that IN cord-329245-6tj2k1yn 6 19 interactions interaction NNS cord-329245-6tj2k1yn 6 20 between between IN cord-329245-6tj2k1yn 6 21 these these DT cord-329245-6tj2k1yn 6 22 proteins protein NNS cord-329245-6tj2k1yn 6 23 play play VBP cord-329245-6tj2k1yn 6 24 a a DT cord-329245-6tj2k1yn 6 25 critical critical JJ cord-329245-6tj2k1yn 6 26 role role NN cord-329245-6tj2k1yn 6 27 in in IN cord-329245-6tj2k1yn 6 28 coronavirus coronavirus NN cord-329245-6tj2k1yn 6 29 assembly assembly NN cord-329245-6tj2k1yn 6 30 . . . cord-329245-6tj2k1yn 7 1 We -PRON- PRP cord-329245-6tj2k1yn 7 2 studied study VBD cord-329245-6tj2k1yn 7 3 interactions interaction NNS cord-329245-6tj2k1yn 7 4 between between IN cord-329245-6tj2k1yn 7 5 the the DT cord-329245-6tj2k1yn 7 6 infectious infectious JJ cord-329245-6tj2k1yn 7 7 bronchitis bronchitis NN cord-329245-6tj2k1yn 7 8 virus virus NN cord-329245-6tj2k1yn 7 9 ( ( -LRB- cord-329245-6tj2k1yn 7 10 IBV IBV NNP cord-329245-6tj2k1yn 7 11 ) ) -RRB- cord-329245-6tj2k1yn 7 12 E E NNP cord-329245-6tj2k1yn 7 13 and and CC cord-329245-6tj2k1yn 7 14 M M NNP cord-329245-6tj2k1yn 7 15 proteins protein NNS cord-329245-6tj2k1yn 7 16 using use VBG cord-329245-6tj2k1yn 7 17 in in IN cord-329245-6tj2k1yn 7 18 vivo vivo NN cord-329245-6tj2k1yn 7 19 crosslinking crosslinking NN cord-329245-6tj2k1yn 7 20 and and CC cord-329245-6tj2k1yn 7 21 VLP VLP NNP cord-329245-6tj2k1yn 7 22 assembly assembly NN cord-329245-6tj2k1yn 7 23 assays assay NNS cord-329245-6tj2k1yn 7 24 . . . cord-329245-6tj2k1yn 8 1 We -PRON- PRP cord-329245-6tj2k1yn 8 2 show show VBP cord-329245-6tj2k1yn 8 3 that that IN cord-329245-6tj2k1yn 8 4 IBV IBV NNP cord-329245-6tj2k1yn 8 5 E E NNP cord-329245-6tj2k1yn 8 6 and and CC cord-329245-6tj2k1yn 8 7 M M NNP cord-329245-6tj2k1yn 8 8 can can MD cord-329245-6tj2k1yn 8 9 be be VB cord-329245-6tj2k1yn 8 10 crosslinked crosslinke VBN cord-329245-6tj2k1yn 8 11 to to IN cord-329245-6tj2k1yn 8 12 each each DT cord-329245-6tj2k1yn 8 13 other other JJ cord-329245-6tj2k1yn 8 14 in in IN cord-329245-6tj2k1yn 8 15 IBV IBV NNP cord-329245-6tj2k1yn 8 16 - - HYPH cord-329245-6tj2k1yn 8 17 infected infect VBN cord-329245-6tj2k1yn 8 18 and and CC cord-329245-6tj2k1yn 8 19 transfected transfecte VBN cord-329245-6tj2k1yn 8 20 cells cell NNS cord-329245-6tj2k1yn 8 21 , , , cord-329245-6tj2k1yn 8 22 indicating indicate VBG cord-329245-6tj2k1yn 8 23 that that IN cord-329245-6tj2k1yn 8 24 they -PRON- PRP cord-329245-6tj2k1yn 8 25 interact interact VBP cord-329245-6tj2k1yn 8 26 . . . cord-329245-6tj2k1yn 9 1 The the DT cord-329245-6tj2k1yn 9 2 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 9 3 tails tail NNS cord-329245-6tj2k1yn 9 4 of of IN cord-329245-6tj2k1yn 9 5 both both DT cord-329245-6tj2k1yn 9 6 proteins protein NNS cord-329245-6tj2k1yn 9 7 are be VBP cord-329245-6tj2k1yn 9 8 important important JJ cord-329245-6tj2k1yn 9 9 for for IN cord-329245-6tj2k1yn 9 10 this this DT cord-329245-6tj2k1yn 9 11 interaction interaction NN cord-329245-6tj2k1yn 9 12 . . . cord-329245-6tj2k1yn 10 1 We -PRON- PRP cord-329245-6tj2k1yn 10 2 also also RB cord-329245-6tj2k1yn 10 3 examined examine VBD cord-329245-6tj2k1yn 10 4 the the DT cord-329245-6tj2k1yn 10 5 ability ability NN cord-329245-6tj2k1yn 10 6 of of IN cord-329245-6tj2k1yn 10 7 the the DT cord-329245-6tj2k1yn 10 8 mutant mutant JJ cord-329245-6tj2k1yn 10 9 and and CC cord-329245-6tj2k1yn 10 10 chimeric chimeric JJ cord-329245-6tj2k1yn 10 11 E e NN cord-329245-6tj2k1yn 10 12 and and CC cord-329245-6tj2k1yn 10 13 M M NNP cord-329245-6tj2k1yn 10 14 proteins protein NNS cord-329245-6tj2k1yn 10 15 to to TO cord-329245-6tj2k1yn 10 16 form form VB cord-329245-6tj2k1yn 10 17 VLPs vlp NNS cord-329245-6tj2k1yn 10 18 . . . cord-329245-6tj2k1yn 11 1 IBV IBV NNP cord-329245-6tj2k1yn 11 2 M m CD cord-329245-6tj2k1yn 11 3 proteins protein NNS cord-329245-6tj2k1yn 11 4 that that WDT cord-329245-6tj2k1yn 11 5 are be VBP cord-329245-6tj2k1yn 11 6 missing miss VBG cord-329245-6tj2k1yn 11 7 portions portion NNS cord-329245-6tj2k1yn 11 8 of of IN cord-329245-6tj2k1yn 11 9 their -PRON- PRP$ cord-329245-6tj2k1yn 11 10 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 11 11 tails tail NNS cord-329245-6tj2k1yn 11 12 or or CC cord-329245-6tj2k1yn 11 13 transmembrane transmembrane NN cord-329245-6tj2k1yn 11 14 regions region NNS cord-329245-6tj2k1yn 11 15 were be VBD cord-329245-6tj2k1yn 11 16 not not RB cord-329245-6tj2k1yn 11 17 able able JJ cord-329245-6tj2k1yn 11 18 to to TO cord-329245-6tj2k1yn 11 19 support support VB cord-329245-6tj2k1yn 11 20 VLP VLP NNP cord-329245-6tj2k1yn 11 21 formation formation NN cord-329245-6tj2k1yn 11 22 , , , cord-329245-6tj2k1yn 11 23 regardless regardless RB cord-329245-6tj2k1yn 11 24 of of IN cord-329245-6tj2k1yn 11 25 their -PRON- PRP$ cord-329245-6tj2k1yn 11 26 ability ability NN cord-329245-6tj2k1yn 11 27 to to TO cord-329245-6tj2k1yn 11 28 be be VB cord-329245-6tj2k1yn 11 29 crosslinked crosslinke VBN cord-329245-6tj2k1yn 11 30 to to IN cord-329245-6tj2k1yn 11 31 IBV IBV NNP cord-329245-6tj2k1yn 11 32 E. E. NNP cord-329245-6tj2k1yn 11 33 Interactions Interactions NNPS cord-329245-6tj2k1yn 11 34 between between IN cord-329245-6tj2k1yn 11 35 the the DT cord-329245-6tj2k1yn 11 36 E e NN cord-329245-6tj2k1yn 11 37 and and CC cord-329245-6tj2k1yn 11 38 M M NNP cord-329245-6tj2k1yn 11 39 proteins protein NNS cord-329245-6tj2k1yn 11 40 and and CC cord-329245-6tj2k1yn 11 41 the the DT cord-329245-6tj2k1yn 11 42 membrane membrane NN cord-329245-6tj2k1yn 11 43 bilayer bilayer NN cord-329245-6tj2k1yn 11 44 are be VBP cord-329245-6tj2k1yn 11 45 likely likely JJ cord-329245-6tj2k1yn 11 46 to to TO cord-329245-6tj2k1yn 11 47 play play VB cord-329245-6tj2k1yn 11 48 an an DT cord-329245-6tj2k1yn 11 49 important important JJ cord-329245-6tj2k1yn 11 50 role role NN cord-329245-6tj2k1yn 11 51 in in IN cord-329245-6tj2k1yn 11 52 VLP VLP NNP cord-329245-6tj2k1yn 11 53 formation formation NN cord-329245-6tj2k1yn 11 54 and and CC cord-329245-6tj2k1yn 11 55 virus virus NN cord-329245-6tj2k1yn 11 56 budding bud VBG cord-329245-6tj2k1yn 11 57 . . . cord-329245-6tj2k1yn 12 1 Enveloped envelop VBN cord-329245-6tj2k1yn 12 2 viruses virus NNS cord-329245-6tj2k1yn 12 3 acquire acquire VBP cord-329245-6tj2k1yn 12 4 their -PRON- PRP$ cord-329245-6tj2k1yn 12 5 lipid lipid NN cord-329245-6tj2k1yn 12 6 membranes membrane NNS cord-329245-6tj2k1yn 12 7 from from IN cord-329245-6tj2k1yn 12 8 those those DT cord-329245-6tj2k1yn 12 9 of of IN cord-329245-6tj2k1yn 12 10 the the DT cord-329245-6tj2k1yn 12 11 host host NN cord-329245-6tj2k1yn 12 12 cell cell NN cord-329245-6tj2k1yn 12 13 in in IN cord-329245-6tj2k1yn 12 14 a a DT cord-329245-6tj2k1yn 12 15 process process NN cord-329245-6tj2k1yn 12 16 known know VBN cord-329245-6tj2k1yn 12 17 as as IN cord-329245-6tj2k1yn 12 18 budding bud VBG cord-329245-6tj2k1yn 12 19 , , , cord-329245-6tj2k1yn 12 20 in in IN cord-329245-6tj2k1yn 12 21 which which WDT cord-329245-6tj2k1yn 12 22 the the DT cord-329245-6tj2k1yn 12 23 virus virus NN cord-329245-6tj2k1yn 12 24 envelope envelope NN cord-329245-6tj2k1yn 12 25 proteins protein NNS cord-329245-6tj2k1yn 12 26 accumulate accumulate VBP cord-329245-6tj2k1yn 12 27 at at IN cord-329245-6tj2k1yn 12 28 the the DT cord-329245-6tj2k1yn 12 29 appropriate appropriate JJ cord-329245-6tj2k1yn 12 30 membrane membrane NN cord-329245-6tj2k1yn 12 31 and and CC cord-329245-6tj2k1yn 12 32 cooperate cooperate VB cord-329245-6tj2k1yn 12 33 with with IN cord-329245-6tj2k1yn 12 34 the the DT cord-329245-6tj2k1yn 12 35 nucleocapsid nucleocapsid NN cord-329245-6tj2k1yn 12 36 and and CC cord-329245-6tj2k1yn 12 37 other other JJ cord-329245-6tj2k1yn 12 38 viral viral JJ cord-329245-6tj2k1yn 12 39 components component NNS cord-329245-6tj2k1yn 12 40 to to TO cord-329245-6tj2k1yn 12 41 induce induce VB cord-329245-6tj2k1yn 12 42 membrane membrane NN cord-329245-6tj2k1yn 12 43 curvature curvature NN cord-329245-6tj2k1yn 12 44 and and CC cord-329245-6tj2k1yn 12 45 pinching pinch VBG cord-329245-6tj2k1yn 12 46 ( ( -LRB- cord-329245-6tj2k1yn 12 47 Garoff Garoff NNP cord-329245-6tj2k1yn 12 48 et et FW cord-329245-6tj2k1yn 12 49 al al NNP cord-329245-6tj2k1yn 12 50 . . NNP cord-329245-6tj2k1yn 12 51 , , , cord-329245-6tj2k1yn 12 52 1998 1998 CD cord-329245-6tj2k1yn 12 53 ) ) -RRB- cord-329245-6tj2k1yn 12 54 . . . cord-329245-6tj2k1yn 13 1 Most most RBS cord-329245-6tj2k1yn 13 2 well well RB cord-329245-6tj2k1yn 13 3 - - HYPH cord-329245-6tj2k1yn 13 4 studied study VBN cord-329245-6tj2k1yn 13 5 enveloped envelop VBN cord-329245-6tj2k1yn 13 6 viruses virus NNS cord-329245-6tj2k1yn 13 7 , , , cord-329245-6tj2k1yn 13 8 such such JJ cord-329245-6tj2k1yn 13 9 as as IN cord-329245-6tj2k1yn 13 10 influenza influenza NN cord-329245-6tj2k1yn 13 11 and and CC cord-329245-6tj2k1yn 13 12 retroviruses retrovirus NNS cord-329245-6tj2k1yn 13 13 , , , cord-329245-6tj2k1yn 13 14 bud bud NN cord-329245-6tj2k1yn 13 15 from from IN cord-329245-6tj2k1yn 13 16 the the DT cord-329245-6tj2k1yn 13 17 plasma plasma NN cord-329245-6tj2k1yn 13 18 membrane membrane NN cord-329245-6tj2k1yn 13 19 of of IN cord-329245-6tj2k1yn 13 20 cells cell NNS cord-329245-6tj2k1yn 13 21 , , , cord-329245-6tj2k1yn 13 22 which which WDT cord-329245-6tj2k1yn 13 23 results result VBZ cord-329245-6tj2k1yn 13 24 in in IN cord-329245-6tj2k1yn 13 25 the the DT cord-329245-6tj2k1yn 13 26 release release NN cord-329245-6tj2k1yn 13 27 of of IN cord-329245-6tj2k1yn 13 28 virus virus NN cord-329245-6tj2k1yn 13 29 particles particle NNS cord-329245-6tj2k1yn 13 30 from from IN cord-329245-6tj2k1yn 13 31 the the DT cord-329245-6tj2k1yn 13 32 host host NN cord-329245-6tj2k1yn 13 33 cell cell NN cord-329245-6tj2k1yn 13 34 as as RB cord-329245-6tj2k1yn 13 35 soon soon RB cord-329245-6tj2k1yn 13 36 as as IN cord-329245-6tj2k1yn 13 37 the the DT cord-329245-6tj2k1yn 13 38 pinching pinching NN cord-329245-6tj2k1yn 13 39 process process NN cord-329245-6tj2k1yn 13 40 is be VBZ cord-329245-6tj2k1yn 13 41 complete complete JJ cord-329245-6tj2k1yn 13 42 . . . cord-329245-6tj2k1yn 14 1 However however RB cord-329245-6tj2k1yn 14 2 , , , cord-329245-6tj2k1yn 14 3 the the DT cord-329245-6tj2k1yn 14 4 envelopes envelope NNS cord-329245-6tj2k1yn 14 5 of of IN cord-329245-6tj2k1yn 14 6 other other JJ cord-329245-6tj2k1yn 14 7 viruses virus NNS cord-329245-6tj2k1yn 14 8 are be VBP cord-329245-6tj2k1yn 14 9 derived derive VBN cord-329245-6tj2k1yn 14 10 by by IN cord-329245-6tj2k1yn 14 11 budding bud VBG cord-329245-6tj2k1yn 14 12 into into IN cord-329245-6tj2k1yn 14 13 the the DT cord-329245-6tj2k1yn 14 14 lumen lumen NN cord-329245-6tj2k1yn 14 15 of of IN cord-329245-6tj2k1yn 14 16 intracellular intracellular JJ cord-329245-6tj2k1yn 14 17 secretory secretory JJ cord-329245-6tj2k1yn 14 18 compartments compartment NNS cord-329245-6tj2k1yn 14 19 , , , cord-329245-6tj2k1yn 14 20 such such JJ cord-329245-6tj2k1yn 14 21 as as IN cord-329245-6tj2k1yn 14 22 the the DT cord-329245-6tj2k1yn 14 23 endoplasmic endoplasmic NN cord-329245-6tj2k1yn 14 24 reticulum reticulum NN cord-329245-6tj2k1yn 14 25 ( ( -LRB- cord-329245-6tj2k1yn 14 26 ER ER NNP cord-329245-6tj2k1yn 14 27 ) ) -RRB- cord-329245-6tj2k1yn 14 28 and and CC cord-329245-6tj2k1yn 14 29 Golgi Golgi NNP cord-329245-6tj2k1yn 14 30 apparatus apparatus NN cord-329245-6tj2k1yn 14 31 ( ( -LRB- cord-329245-6tj2k1yn 14 32 Griffiths Griffiths NNP cord-329245-6tj2k1yn 14 33 and and CC cord-329245-6tj2k1yn 14 34 Rottier Rottier NNP cord-329245-6tj2k1yn 14 35 , , , cord-329245-6tj2k1yn 14 36 1992 1992 CD cord-329245-6tj2k1yn 14 37 ) ) -RRB- cord-329245-6tj2k1yn 14 38 . . . cord-329245-6tj2k1yn 15 1 Intracellular Intracellular NNP cord-329245-6tj2k1yn 15 2 budding budding NN cord-329245-6tj2k1yn 15 3 is be VBZ cord-329245-6tj2k1yn 15 4 a a DT cord-329245-6tj2k1yn 15 5 seemingly seemingly RB cord-329245-6tj2k1yn 15 6 more more RBR cord-329245-6tj2k1yn 15 7 complex complex JJ cord-329245-6tj2k1yn 15 8 way way NN cord-329245-6tj2k1yn 15 9 of of IN cord-329245-6tj2k1yn 15 10 obtaining obtain VBG cord-329245-6tj2k1yn 15 11 a a DT cord-329245-6tj2k1yn 15 12 lipid lipid NN cord-329245-6tj2k1yn 15 13 envelope envelope NN cord-329245-6tj2k1yn 15 14 , , , cord-329245-6tj2k1yn 15 15 since since IN cord-329245-6tj2k1yn 15 16 virus virus NN cord-329245-6tj2k1yn 15 17 particles particle NNS cord-329245-6tj2k1yn 15 18 are be VBP cord-329245-6tj2k1yn 15 19 contained contain VBN cord-329245-6tj2k1yn 15 20 within within IN cord-329245-6tj2k1yn 15 21 intracellular intracellular JJ cord-329245-6tj2k1yn 15 22 membrane membrane NN cord-329245-6tj2k1yn 15 23 - - HYPH cord-329245-6tj2k1yn 15 24 bound bind VBN cord-329245-6tj2k1yn 15 25 compartments compartment NNS cord-329245-6tj2k1yn 15 26 as as IN cord-329245-6tj2k1yn 15 27 a a DT cord-329245-6tj2k1yn 15 28 result result NN cord-329245-6tj2k1yn 15 29 of of IN cord-329245-6tj2k1yn 15 30 budding bud VBG cord-329245-6tj2k1yn 15 31 . . . cord-329245-6tj2k1yn 16 1 The the DT cord-329245-6tj2k1yn 16 2 newly newly RB cord-329245-6tj2k1yn 16 3 budded bud VBN cord-329245-6tj2k1yn 16 4 viruses virus NNS cord-329245-6tj2k1yn 16 5 presumably presumably RB cord-329245-6tj2k1yn 16 6 exploit exploit VBP cord-329245-6tj2k1yn 16 7 the the DT cord-329245-6tj2k1yn 16 8 cellular cellular JJ cord-329245-6tj2k1yn 16 9 secretory secretory JJ cord-329245-6tj2k1yn 16 10 pathway pathway NN cord-329245-6tj2k1yn 16 11 to to TO cord-329245-6tj2k1yn 16 12 exit exit VB cord-329245-6tj2k1yn 16 13 the the DT cord-329245-6tj2k1yn 16 14 cell cell NN cord-329245-6tj2k1yn 16 15 for for IN cord-329245-6tj2k1yn 16 16 a a DT cord-329245-6tj2k1yn 16 17 new new JJ cord-329245-6tj2k1yn 16 18 round round NN cord-329245-6tj2k1yn 16 19 of of IN cord-329245-6tj2k1yn 16 20 infection infection NN cord-329245-6tj2k1yn 16 21 . . . cord-329245-6tj2k1yn 17 1 The the DT cord-329245-6tj2k1yn 17 2 reasons reason NNS cord-329245-6tj2k1yn 17 3 that that WDT cord-329245-6tj2k1yn 17 4 some some DT cord-329245-6tj2k1yn 17 5 enveloped envelop VBN cord-329245-6tj2k1yn 17 6 viruses virus NNS cord-329245-6tj2k1yn 17 7 have have VBP cord-329245-6tj2k1yn 17 8 evolved evolve VBN cord-329245-6tj2k1yn 17 9 to to TO cord-329245-6tj2k1yn 17 10 bud bud VB cord-329245-6tj2k1yn 17 11 inside inside IN cord-329245-6tj2k1yn 17 12 the the DT cord-329245-6tj2k1yn 17 13 cell cell NN cord-329245-6tj2k1yn 17 14 are be VBP cord-329245-6tj2k1yn 17 15 not not RB cord-329245-6tj2k1yn 17 16 known know VBN cord-329245-6tj2k1yn 17 17 . . . cord-329245-6tj2k1yn 18 1 Several several JJ cord-329245-6tj2k1yn 18 2 clinically clinically RB cord-329245-6tj2k1yn 18 3 important important JJ cord-329245-6tj2k1yn 18 4 viruses virus NNS cord-329245-6tj2k1yn 18 5 bud bud VBP cord-329245-6tj2k1yn 18 6 into into IN cord-329245-6tj2k1yn 18 7 intracellular intracellular JJ cord-329245-6tj2k1yn 18 8 membranes membrane NNS cord-329245-6tj2k1yn 18 9 . . . cord-329245-6tj2k1yn 19 1 Viruses virus NNS cord-329245-6tj2k1yn 19 2 of of IN cord-329245-6tj2k1yn 19 3 the the DT cord-329245-6tj2k1yn 19 4 family family NN cord-329245-6tj2k1yn 19 5 Flaviviridae Flaviviridae NNP cord-329245-6tj2k1yn 19 6 , , , cord-329245-6tj2k1yn 19 7 which which WDT cord-329245-6tj2k1yn 19 8 include include VBP cord-329245-6tj2k1yn 19 9 hepatitis hepatitis NN cord-329245-6tj2k1yn 19 10 C C NNP cord-329245-6tj2k1yn 19 11 virus virus NN cord-329245-6tj2k1yn 19 12 and and CC cord-329245-6tj2k1yn 19 13 Kunjin Kunjin NNP cord-329245-6tj2k1yn 19 14 virus virus NN cord-329245-6tj2k1yn 19 15 , , , cord-329245-6tj2k1yn 19 16 derive derive VBP cord-329245-6tj2k1yn 19 17 their -PRON- PRP$ cord-329245-6tj2k1yn 19 18 lipid lipid NN cord-329245-6tj2k1yn 19 19 envelopes envelope NNS cord-329245-6tj2k1yn 19 20 by by IN cord-329245-6tj2k1yn 19 21 budding bud VBG cord-329245-6tj2k1yn 19 22 into into IN cord-329245-6tj2k1yn 19 23 the the DT cord-329245-6tj2k1yn 19 24 lumen lumen NN cord-329245-6tj2k1yn 19 25 of of IN cord-329245-6tj2k1yn 19 26 the the DT cord-329245-6tj2k1yn 19 27 ER ER NNP cord-329245-6tj2k1yn 20 1 ( ( -LRB- cord-329245-6tj2k1yn 20 2 Dubois Dubois NNP cord-329245-6tj2k1yn 20 3 - - HYPH cord-329245-6tj2k1yn 20 4 Dalcq Dalcq NNP cord-329245-6tj2k1yn 20 5 et et FW cord-329245-6tj2k1yn 20 6 al al NNP cord-329245-6tj2k1yn 20 7 . . . cord-329245-6tj2k1yn 21 1 , , , cord-329245-6tj2k1yn 21 2 1984 1984 CD cord-329245-6tj2k1yn 21 3 ; ; : cord-329245-6tj2k1yn 21 4 Mackenzie Mackenzie NNP cord-329245-6tj2k1yn 21 5 and and CC cord-329245-6tj2k1yn 21 6 Westaway Westaway NNP cord-329245-6tj2k1yn 21 7 , , , cord-329245-6tj2k1yn 21 8 2001 2001 CD cord-329245-6tj2k1yn 21 9 ) ) -RRB- cord-329245-6tj2k1yn 21 10 . . . cord-329245-6tj2k1yn 22 1 As as IN cord-329245-6tj2k1yn 22 2 another another DT cord-329245-6tj2k1yn 22 3 example example NN cord-329245-6tj2k1yn 22 4 , , , cord-329245-6tj2k1yn 22 5 the the DT cord-329245-6tj2k1yn 22 6 hemorrhagic hemorrhagic JJ cord-329245-6tj2k1yn 22 7 fever fever NN cord-329245-6tj2k1yn 22 8 - - HYPH cord-329245-6tj2k1yn 22 9 causing cause VBG cord-329245-6tj2k1yn 22 10 Hantaviruses Hantaviruses NNP cord-329245-6tj2k1yn 22 11 , , , cord-329245-6tj2k1yn 22 12 members member NNS cord-329245-6tj2k1yn 22 13 of of IN cord-329245-6tj2k1yn 22 14 the the DT cord-329245-6tj2k1yn 22 15 family family NN cord-329245-6tj2k1yn 22 16 Bunyaviridae Bunyaviridae NNP cord-329245-6tj2k1yn 22 17 , , , cord-329245-6tj2k1yn 22 18 bud bud NN cord-329245-6tj2k1yn 22 19 into into IN cord-329245-6tj2k1yn 22 20 the the DT cord-329245-6tj2k1yn 22 21 membranes membrane NNS cord-329245-6tj2k1yn 22 22 of of IN cord-329245-6tj2k1yn 22 23 the the DT cord-329245-6tj2k1yn 22 24 Golgi Golgi NNP cord-329245-6tj2k1yn 22 25 complex complex NN cord-329245-6tj2k1yn 23 1 ( ( -LRB- cord-329245-6tj2k1yn 23 2 Hung Hung NNP cord-329245-6tj2k1yn 23 3 et et FW cord-329245-6tj2k1yn 23 4 al al NNP cord-329245-6tj2k1yn 23 5 . . NNP cord-329245-6tj2k1yn 23 6 , , , cord-329245-6tj2k1yn 23 7 1985 1985 CD cord-329245-6tj2k1yn 24 1 ; ; : cord-329245-6tj2k1yn 24 2 Pettersson Pettersson NNP cord-329245-6tj2k1yn 24 3 , , , cord-329245-6tj2k1yn 24 4 1991 1991 CD cord-329245-6tj2k1yn 24 5 ) ) -RRB- cord-329245-6tj2k1yn 24 6 . . . cord-329245-6tj2k1yn 25 1 The the DT cord-329245-6tj2k1yn 25 2 advantage advantage NN cord-329245-6tj2k1yn 25 3 provided provide VBN cord-329245-6tj2k1yn 25 4 by by IN cord-329245-6tj2k1yn 25 5 intracellular intracellular JJ cord-329245-6tj2k1yn 25 6 budding budding NN cord-329245-6tj2k1yn 25 7 remains remain VBZ cord-329245-6tj2k1yn 25 8 an an DT cord-329245-6tj2k1yn 25 9 interesting interesting JJ cord-329245-6tj2k1yn 25 10 and and CC cord-329245-6tj2k1yn 25 11 important important JJ cord-329245-6tj2k1yn 25 12 question question NN cord-329245-6tj2k1yn 25 13 in in IN cord-329245-6tj2k1yn 25 14 the the DT cord-329245-6tj2k1yn 25 15 biology biology NN cord-329245-6tj2k1yn 25 16 of of IN cord-329245-6tj2k1yn 25 17 these these DT cord-329245-6tj2k1yn 25 18 and and CC cord-329245-6tj2k1yn 25 19 other other JJ cord-329245-6tj2k1yn 25 20 viruses virus NNS cord-329245-6tj2k1yn 25 21 that that WDT cord-329245-6tj2k1yn 25 22 obtain obtain VBP cord-329245-6tj2k1yn 25 23 their -PRON- PRP$ cord-329245-6tj2k1yn 25 24 envelopes envelope NNS cord-329245-6tj2k1yn 25 25 from from IN cord-329245-6tj2k1yn 25 26 intracellular intracellular JJ cord-329245-6tj2k1yn 25 27 membranes membrane NNS cord-329245-6tj2k1yn 25 28 . . . cord-329245-6tj2k1yn 26 1 Coronaviruses coronaviruse NNS cord-329245-6tj2k1yn 26 2 are be VBP cord-329245-6tj2k1yn 26 3 positive positive JJ cord-329245-6tj2k1yn 26 4 - - HYPH cord-329245-6tj2k1yn 26 5 stranded strand VBN cord-329245-6tj2k1yn 26 6 RNA RNA NNP cord-329245-6tj2k1yn 26 7 viruses virus NNS cord-329245-6tj2k1yn 26 8 that that WDT cord-329245-6tj2k1yn 26 9 acquire acquire VBP cord-329245-6tj2k1yn 26 10 their -PRON- PRP$ cord-329245-6tj2k1yn 26 11 membrane membrane NN cord-329245-6tj2k1yn 26 12 envelope envelope NN cord-329245-6tj2k1yn 26 13 by by IN cord-329245-6tj2k1yn 26 14 budding bud VBG cord-329245-6tj2k1yn 26 15 into into IN cord-329245-6tj2k1yn 26 16 the the DT cord-329245-6tj2k1yn 26 17 lumen lumen NN cord-329245-6tj2k1yn 26 18 of of IN cord-329245-6tj2k1yn 26 19 a a DT cord-329245-6tj2k1yn 26 20 pre pre JJ cord-329245-6tj2k1yn 26 21 - - JJ cord-329245-6tj2k1yn 26 22 Golgi Golgi NNP cord-329245-6tj2k1yn 26 23 compartment compartment NN cord-329245-6tj2k1yn 26 24 or or CC cord-329245-6tj2k1yn 26 25 cis cis NNP cord-329245-6tj2k1yn 26 26 - - HYPH cord-329245-6tj2k1yn 26 27 Golgi Golgi NNP cord-329245-6tj2k1yn 26 28 network network NN cord-329245-6tj2k1yn 26 29 ( ( -LRB- cord-329245-6tj2k1yn 26 30 CGN CGN NNP cord-329245-6tj2k1yn 26 31 ) ) -RRB- cord-329245-6tj2k1yn 27 1 ( ( -LRB- cord-329245-6tj2k1yn 27 2 Klumperman Klumperman NNP cord-329245-6tj2k1yn 27 3 et et FW cord-329245-6tj2k1yn 27 4 al al NNP cord-329245-6tj2k1yn 27 5 . . NNP cord-329245-6tj2k1yn 27 6 , , , cord-329245-6tj2k1yn 27 7 1994 1994 CD cord-329245-6tj2k1yn 27 8 ) ) -RRB- cord-329245-6tj2k1yn 27 9 . . . cord-329245-6tj2k1yn 28 1 Accumulating accumulate VBG cord-329245-6tj2k1yn 28 2 evidence evidence NN cord-329245-6tj2k1yn 28 3 indicates indicate VBZ cord-329245-6tj2k1yn 28 4 that that IN cord-329245-6tj2k1yn 28 5 the the DT cord-329245-6tj2k1yn 28 6 E E NNP cord-329245-6tj2k1yn 28 7 and and CC cord-329245-6tj2k1yn 28 8 M M NNP cord-329245-6tj2k1yn 28 9 proteins protein NNS cord-329245-6tj2k1yn 28 10 are be VBP cord-329245-6tj2k1yn 28 11 instrumental instrumental JJ cord-329245-6tj2k1yn 28 12 in in IN cord-329245-6tj2k1yn 28 13 this this DT cord-329245-6tj2k1yn 28 14 process process NN cord-329245-6tj2k1yn 28 15 . . . cord-329245-6tj2k1yn 29 1 Rottier Rottier NNP cord-329245-6tj2k1yn 29 2 and and CC cord-329245-6tj2k1yn 29 3 colleagues colleague NNS cord-329245-6tj2k1yn 29 4 made make VBD cord-329245-6tj2k1yn 29 5 the the DT cord-329245-6tj2k1yn 29 6 initial initial JJ cord-329245-6tj2k1yn 29 7 observation observation NN cord-329245-6tj2k1yn 29 8 that that WDT cord-329245-6tj2k1yn 29 9 mouse mouse NNP cord-329245-6tj2k1yn 29 10 hepatitis hepatitis NN cord-329245-6tj2k1yn 29 11 virus virus NN cord-329245-6tj2k1yn 29 12 ( ( -LRB- cord-329245-6tj2k1yn 29 13 MHV mhv NN cord-329245-6tj2k1yn 29 14 ) ) -RRB- cord-329245-6tj2k1yn 29 15 E E NNP cord-329245-6tj2k1yn 29 16 and and CC cord-329245-6tj2k1yn 29 17 M M NNP cord-329245-6tj2k1yn 29 18 proteins protein NNS cord-329245-6tj2k1yn 29 19 are be VBP cord-329245-6tj2k1yn 29 20 sufficient sufficient JJ cord-329245-6tj2k1yn 29 21 to to TO cord-329245-6tj2k1yn 29 22 produce produce VB cord-329245-6tj2k1yn 29 23 membrane membrane NN cord-329245-6tj2k1yn 29 24 - - HYPH cord-329245-6tj2k1yn 29 25 bound bind VBN cord-329245-6tj2k1yn 29 26 particles particle NNS cord-329245-6tj2k1yn 29 27 very very RB cord-329245-6tj2k1yn 29 28 similar similar JJ cord-329245-6tj2k1yn 29 29 to to IN cord-329245-6tj2k1yn 29 30 virions virion NNS cord-329245-6tj2k1yn 29 31 in in IN cord-329245-6tj2k1yn 29 32 size size NN cord-329245-6tj2k1yn 29 33 and and CC cord-329245-6tj2k1yn 29 34 shape shape NN cord-329245-6tj2k1yn 29 35 ( ( -LRB- cord-329245-6tj2k1yn 29 36 Vennema Vennema NNP cord-329245-6tj2k1yn 29 37 et et FW cord-329245-6tj2k1yn 29 38 al al NNP cord-329245-6tj2k1yn 29 39 . . NNP cord-329245-6tj2k1yn 29 40 , , , cord-329245-6tj2k1yn 29 41 1996 1996 CD cord-329245-6tj2k1yn 29 42 ) ) -RRB- cord-329245-6tj2k1yn 29 43 , , , cord-329245-6tj2k1yn 29 44 and and CC cord-329245-6tj2k1yn 29 45 this this DT cord-329245-6tj2k1yn 29 46 result result NN cord-329245-6tj2k1yn 29 47 has have VBZ cord-329245-6tj2k1yn 29 48 been be VBN cord-329245-6tj2k1yn 29 49 confirmed confirm VBN cord-329245-6tj2k1yn 29 50 with with IN cord-329245-6tj2k1yn 29 51 the the DT cord-329245-6tj2k1yn 29 52 E e NN cord-329245-6tj2k1yn 29 53 and and CC cord-329245-6tj2k1yn 29 54 M M NNP cord-329245-6tj2k1yn 29 55 proteins protein NNS cord-329245-6tj2k1yn 29 56 of of IN cord-329245-6tj2k1yn 29 57 several several JJ cord-329245-6tj2k1yn 29 58 other other JJ cord-329245-6tj2k1yn 29 59 coronaviruses coronaviruse NNS cord-329245-6tj2k1yn 30 1 ( ( -LRB- cord-329245-6tj2k1yn 30 2 Baudoux Baudoux NNP cord-329245-6tj2k1yn 30 3 et et NNP cord-329245-6tj2k1yn 30 4 al al NNP cord-329245-6tj2k1yn 30 5 . . NNP cord-329245-6tj2k1yn 30 6 , , , cord-329245-6tj2k1yn 30 7 1998 1998 CD cord-329245-6tj2k1yn 30 8 ; ; : cord-329245-6tj2k1yn 31 1 Corse Corse NNP cord-329245-6tj2k1yn 31 2 and and CC cord-329245-6tj2k1yn 31 3 Machamer Machamer NNP cord-329245-6tj2k1yn 31 4 , , , cord-329245-6tj2k1yn 31 5 2000 2000 CD cord-329245-6tj2k1yn 31 6 ; ; : cord-329245-6tj2k1yn 31 7 Godeke Godeke NNP cord-329245-6tj2k1yn 31 8 et et FW cord-329245-6tj2k1yn 31 9 al al NNP cord-329245-6tj2k1yn 31 10 . . NNP cord-329245-6tj2k1yn 31 11 , , , cord-329245-6tj2k1yn 31 12 2000 2000 CD cord-329245-6tj2k1yn 31 13 ) ) -RRB- cord-329245-6tj2k1yn 31 14 . . . cord-329245-6tj2k1yn 32 1 These these DT cord-329245-6tj2k1yn 32 2 observations observation NNS cord-329245-6tj2k1yn 32 3 suggest suggest VBP cord-329245-6tj2k1yn 32 4 that that IN cord-329245-6tj2k1yn 32 5 coronavirus coronavirus NN cord-329245-6tj2k1yn 32 6 budding budding NN cord-329245-6tj2k1yn 32 7 is be VBZ cord-329245-6tj2k1yn 32 8 directed direct VBN cord-329245-6tj2k1yn 32 9 by by IN cord-329245-6tj2k1yn 32 10 envelope envelope NN cord-329245-6tj2k1yn 32 11 proteins protein NNS cord-329245-6tj2k1yn 32 12 alone alone RB cord-329245-6tj2k1yn 32 13 , , , cord-329245-6tj2k1yn 32 14 which which WDT cord-329245-6tj2k1yn 32 15 is be VBZ cord-329245-6tj2k1yn 32 16 an an DT cord-329245-6tj2k1yn 32 17 unusual unusual JJ cord-329245-6tj2k1yn 32 18 type type NN cord-329245-6tj2k1yn 32 19 of of IN cord-329245-6tj2k1yn 32 20 enveloped envelop VBN cord-329245-6tj2k1yn 32 21 virus virus NN cord-329245-6tj2k1yn 32 22 assembly assembly NN cord-329245-6tj2k1yn 32 23 ( ( -LRB- cord-329245-6tj2k1yn 32 24 Garoff Garoff NNP cord-329245-6tj2k1yn 32 25 et et FW cord-329245-6tj2k1yn 32 26 al al NNP cord-329245-6tj2k1yn 32 27 . . NNP cord-329245-6tj2k1yn 32 28 , , , cord-329245-6tj2k1yn 32 29 1998 1998 CD cord-329245-6tj2k1yn 32 30 ) ) -RRB- cord-329245-6tj2k1yn 32 31 . . . cord-329245-6tj2k1yn 33 1 The the DT cord-329245-6tj2k1yn 33 2 coronavirus coronavirus NN cord-329245-6tj2k1yn 33 3 E e NN cord-329245-6tj2k1yn 33 4 protein protein NN cord-329245-6tj2k1yn 33 5 appears appear VBZ cord-329245-6tj2k1yn 33 6 to to TO cord-329245-6tj2k1yn 33 7 play play VB cord-329245-6tj2k1yn 33 8 a a DT cord-329245-6tj2k1yn 33 9 critical critical JJ cord-329245-6tj2k1yn 33 10 role role NN cord-329245-6tj2k1yn 33 11 in in IN cord-329245-6tj2k1yn 33 12 the the DT cord-329245-6tj2k1yn 33 13 budding bud VBG cord-329245-6tj2k1yn 33 14 step step NN cord-329245-6tj2k1yn 33 15 , , , cord-329245-6tj2k1yn 33 16 since since IN cord-329245-6tj2k1yn 33 17 mutations mutation NNS cord-329245-6tj2k1yn 33 18 introduced introduce VBN cord-329245-6tj2k1yn 33 19 into into IN cord-329245-6tj2k1yn 33 20 the the DT cord-329245-6tj2k1yn 33 21 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 33 22 tail tail NN cord-329245-6tj2k1yn 33 23 of of IN cord-329245-6tj2k1yn 33 24 MHV MHV NNP cord-329245-6tj2k1yn 33 25 E E NNP cord-329245-6tj2k1yn 33 26 by by IN cord-329245-6tj2k1yn 33 27 targeted target VBN cord-329245-6tj2k1yn 33 28 RNA RNA NNP cord-329245-6tj2k1yn 33 29 recombination recombination NN cord-329245-6tj2k1yn 33 30 result result NN cord-329245-6tj2k1yn 33 31 in in IN cord-329245-6tj2k1yn 33 32 elongated elongated JJ cord-329245-6tj2k1yn 33 33 virions virion NNS cord-329245-6tj2k1yn 33 34 ( ( -LRB- cord-329245-6tj2k1yn 33 35 Fischer Fischer NNP cord-329245-6tj2k1yn 33 36 et et NNP cord-329245-6tj2k1yn 33 37 al al NNP cord-329245-6tj2k1yn 33 38 . . NNP cord-329245-6tj2k1yn 33 39 , , , cord-329245-6tj2k1yn 33 40 1998 1998 CD cord-329245-6tj2k1yn 33 41 ) ) -RRB- cord-329245-6tj2k1yn 33 42 . . . cord-329245-6tj2k1yn 34 1 Indeed indeed RB cord-329245-6tj2k1yn 34 2 , , , cord-329245-6tj2k1yn 34 3 it -PRON- PRP cord-329245-6tj2k1yn 34 4 has have VBZ cord-329245-6tj2k1yn 34 5 been be VBN cord-329245-6tj2k1yn 34 6 shown show VBN cord-329245-6tj2k1yn 34 7 that that IN cord-329245-6tj2k1yn 34 8 both both CC cord-329245-6tj2k1yn 34 9 the the DT cord-329245-6tj2k1yn 34 10 MHV MHV NNP cord-329245-6tj2k1yn 34 11 E E NNS cord-329245-6tj2k1yn 34 12 and and CC cord-329245-6tj2k1yn 34 13 the the DT cord-329245-6tj2k1yn 34 14 infectious infectious JJ cord-329245-6tj2k1yn 34 15 bronchitis bronchitis NN cord-329245-6tj2k1yn 34 16 virus virus NN cord-329245-6tj2k1yn 34 17 ( ( -LRB- cord-329245-6tj2k1yn 34 18 IBV IBV NNP cord-329245-6tj2k1yn 34 19 ) ) -RRB- cord-329245-6tj2k1yn 34 20 E e NN cord-329245-6tj2k1yn 34 21 proteins protein NNS cord-329245-6tj2k1yn 34 22 are be VBP cord-329245-6tj2k1yn 34 23 sufficient sufficient JJ cord-329245-6tj2k1yn 34 24 for for IN cord-329245-6tj2k1yn 34 25 formation formation NN cord-329245-6tj2k1yn 34 26 of of IN cord-329245-6tj2k1yn 34 27 the the DT cord-329245-6tj2k1yn 34 28 virus virus NN cord-329245-6tj2k1yn 34 29 - - HYPH cord-329245-6tj2k1yn 34 30 like like JJ cord-329245-6tj2k1yn 34 31 particles particle NNS cord-329245-6tj2k1yn 34 32 ( ( -LRB- cord-329245-6tj2k1yn 34 33 VLPs VLPs NNP cord-329245-6tj2k1yn 34 34 ) ) -RRB- cord-329245-6tj2k1yn 34 35 described describe VBN cord-329245-6tj2k1yn 34 36 above above IN cord-329245-6tj2k1yn 34 37 ( ( -LRB- cord-329245-6tj2k1yn 34 38 Corse Corse NNP cord-329245-6tj2k1yn 34 39 and and CC cord-329245-6tj2k1yn 34 40 Machamer Machamer NNP cord-329245-6tj2k1yn 34 41 , , , cord-329245-6tj2k1yn 34 42 2000 2000 CD cord-329245-6tj2k1yn 34 43 ; ; : cord-329245-6tj2k1yn 34 44 Maeda Maeda NNP cord-329245-6tj2k1yn 34 45 et et NNP cord-329245-6tj2k1yn 34 46 al al NNP cord-329245-6tj2k1yn 34 47 . . NNP cord-329245-6tj2k1yn 34 48 , , , cord-329245-6tj2k1yn 34 49 1999 1999 CD cord-329245-6tj2k1yn 34 50 ) ) -RRB- cord-329245-6tj2k1yn 34 51 , , , cord-329245-6tj2k1yn 34 52 although although IN cord-329245-6tj2k1yn 34 53 the the DT cord-329245-6tj2k1yn 34 54 efficiency efficiency NN cord-329245-6tj2k1yn 34 55 probably probably RB cord-329245-6tj2k1yn 34 56 varies vary VBZ cord-329245-6tj2k1yn 34 57 with with IN cord-329245-6tj2k1yn 34 58 cell cell NN cord-329245-6tj2k1yn 34 59 type type NN cord-329245-6tj2k1yn 34 60 and and CC cord-329245-6tj2k1yn 34 61 protein protein NN cord-329245-6tj2k1yn 34 62 expression expression NN cord-329245-6tj2k1yn 34 63 system system NN cord-329245-6tj2k1yn 34 64 . . . cord-329245-6tj2k1yn 35 1 We -PRON- PRP cord-329245-6tj2k1yn 35 2 are be VBP cord-329245-6tj2k1yn 35 3 interested interested JJ cord-329245-6tj2k1yn 35 4 in in IN cord-329245-6tj2k1yn 35 5 understanding understand VBG cord-329245-6tj2k1yn 35 6 the the DT cord-329245-6tj2k1yn 35 7 mechanisms mechanism NNS cord-329245-6tj2k1yn 35 8 by by IN cord-329245-6tj2k1yn 35 9 which which WDT cord-329245-6tj2k1yn 35 10 the the DT cord-329245-6tj2k1yn 35 11 avian avian JJ cord-329245-6tj2k1yn 35 12 coronavirus coronavirus NN cord-329245-6tj2k1yn 35 13 IBV IBV NNP cord-329245-6tj2k1yn 35 14 selects select VBZ cord-329245-6tj2k1yn 35 15 a a DT cord-329245-6tj2k1yn 35 16 bud bud NN cord-329245-6tj2k1yn 35 17 site site NN cord-329245-6tj2k1yn 35 18 and and CC cord-329245-6tj2k1yn 35 19 assembles assemble VBZ cord-329245-6tj2k1yn 35 20 in in IN cord-329245-6tj2k1yn 35 21 CGN CGN NNP cord-329245-6tj2k1yn 35 22 membranes membrane NNS cord-329245-6tj2k1yn 35 23 . . . cord-329245-6tj2k1yn 36 1 We -PRON- PRP cord-329245-6tj2k1yn 36 2 have have VBP cord-329245-6tj2k1yn 36 3 previously previously RB cord-329245-6tj2k1yn 36 4 shown show VBN cord-329245-6tj2k1yn 36 5 that that IN cord-329245-6tj2k1yn 36 6 the the DT cord-329245-6tj2k1yn 36 7 IBV IBV NNP cord-329245-6tj2k1yn 36 8 M M NNP cord-329245-6tj2k1yn 36 9 and and CC cord-329245-6tj2k1yn 36 10 E e NN cord-329245-6tj2k1yn 36 11 proteins protein NNS cord-329245-6tj2k1yn 36 12 independently independently RB cord-329245-6tj2k1yn 36 13 localize localize VBP cord-329245-6tj2k1yn 36 14 to to IN cord-329245-6tj2k1yn 36 15 the the DT cord-329245-6tj2k1yn 36 16 Golgi Golgi NNP cord-329245-6tj2k1yn 36 17 region region NN cord-329245-6tj2k1yn 36 18 of of IN cord-329245-6tj2k1yn 36 19 transfected transfecte VBN cord-329245-6tj2k1yn 36 20 cells cell NNS cord-329245-6tj2k1yn 36 21 ( ( -LRB- cord-329245-6tj2k1yn 36 22 Corse Corse NNP cord-329245-6tj2k1yn 36 23 and and CC cord-329245-6tj2k1yn 36 24 Machamer Machamer NNP cord-329245-6tj2k1yn 36 25 , , , cord-329245-6tj2k1yn 36 26 2000 2000 CD cord-329245-6tj2k1yn 36 27 ; ; : cord-329245-6tj2k1yn 36 28 Machamer Machamer NNP cord-329245-6tj2k1yn 36 29 and and CC cord-329245-6tj2k1yn 36 30 Rose Rose NNP cord-329245-6tj2k1yn 36 31 , , , cord-329245-6tj2k1yn 36 32 1987 1987 CD cord-329245-6tj2k1yn 36 33 ) ) -RRB- cord-329245-6tj2k1yn 36 34 and and CC cord-329245-6tj2k1yn 36 35 characterized characterize VBD cord-329245-6tj2k1yn 36 36 the the DT cord-329245-6tj2k1yn 36 37 Golgi Golgi NNP cord-329245-6tj2k1yn 36 38 targeting targeting NN cord-329245-6tj2k1yn 36 39 signals signal NNS cord-329245-6tj2k1yn 36 40 in in IN cord-329245-6tj2k1yn 36 41 the the DT cord-329245-6tj2k1yn 36 42 two two CD cord-329245-6tj2k1yn 36 43 proteins protein NNS cord-329245-6tj2k1yn 36 44 that that WDT cord-329245-6tj2k1yn 36 45 direct direct VBP cord-329245-6tj2k1yn 36 46 this this DT cord-329245-6tj2k1yn 36 47 localization localization NN cord-329245-6tj2k1yn 36 48 ( ( -LRB- cord-329245-6tj2k1yn 36 49 Corse Corse NNP cord-329245-6tj2k1yn 36 50 and and CC cord-329245-6tj2k1yn 36 51 Machamer Machamer NNP cord-329245-6tj2k1yn 36 52 , , , cord-329245-6tj2k1yn 36 53 2002 2002 CD cord-329245-6tj2k1yn 36 54 ; ; : cord-329245-6tj2k1yn 36 55 Machamer Machamer NNP cord-329245-6tj2k1yn 36 56 et et NNP cord-329245-6tj2k1yn 36 57 al al NNP cord-329245-6tj2k1yn 36 58 . . NNP cord-329245-6tj2k1yn 36 59 , , , cord-329245-6tj2k1yn 36 60 1993 1993 CD cord-329245-6tj2k1yn 36 61 ) ) -RRB- cord-329245-6tj2k1yn 36 62 . . . cord-329245-6tj2k1yn 37 1 In in IN cord-329245-6tj2k1yn 37 2 the the DT cord-329245-6tj2k1yn 37 3 study study NN cord-329245-6tj2k1yn 37 4 described describe VBN cord-329245-6tj2k1yn 37 5 here here RB cord-329245-6tj2k1yn 37 6 , , , cord-329245-6tj2k1yn 37 7 we -PRON- PRP cord-329245-6tj2k1yn 37 8 examined examine VBD cord-329245-6tj2k1yn 37 9 interactions interaction NNS cord-329245-6tj2k1yn 37 10 between between IN cord-329245-6tj2k1yn 37 11 the the DT cord-329245-6tj2k1yn 37 12 IBV IBV NNP cord-329245-6tj2k1yn 37 13 E E NNP cord-329245-6tj2k1yn 37 14 and and CC cord-329245-6tj2k1yn 37 15 M M NNP cord-329245-6tj2k1yn 37 16 proteins protein NNS cord-329245-6tj2k1yn 37 17 in in IN cord-329245-6tj2k1yn 37 18 transfected transfecte VBN cord-329245-6tj2k1yn 37 19 OST7 OST7 NNP cord-329245-6tj2k1yn 37 20 - - HYPH cord-329245-6tj2k1yn 37 21 1 1 CD cord-329245-6tj2k1yn 37 22 cells cell NNS cord-329245-6tj2k1yn 37 23 using use VBG cord-329245-6tj2k1yn 37 24 the the DT cord-329245-6tj2k1yn 37 25 chemical chemical NN cord-329245-6tj2k1yn 37 26 crosslinker crosslinker NN cord-329245-6tj2k1yn 37 27 dithiobis[succinimidyl dithiobis[succinimidyl FW cord-329245-6tj2k1yn 37 28 propionate propionate NNP cord-329245-6tj2k1yn 37 29 ] ] -RRB- cord-329245-6tj2k1yn 38 1 ( ( -LRB- cord-329245-6tj2k1yn 38 2 DSP DSP NNP cord-329245-6tj2k1yn 38 3 ) ) -RRB- cord-329245-6tj2k1yn 38 4 . . . cord-329245-6tj2k1yn 39 1 Using use VBG cord-329245-6tj2k1yn 39 2 mutant mutant JJ cord-329245-6tj2k1yn 39 3 E E NNP cord-329245-6tj2k1yn 39 4 and and CC cord-329245-6tj2k1yn 39 5 M M NNP cord-329245-6tj2k1yn 39 6 proteins protein NNS cord-329245-6tj2k1yn 39 7 that that WDT cord-329245-6tj2k1yn 39 8 are be VBP cord-329245-6tj2k1yn 39 9 correctly correctly RB cord-329245-6tj2k1yn 39 10 targeted target VBN cord-329245-6tj2k1yn 39 11 to to IN cord-329245-6tj2k1yn 39 12 the the DT cord-329245-6tj2k1yn 39 13 Golgi Golgi NNP cord-329245-6tj2k1yn 39 14 complex complex NN cord-329245-6tj2k1yn 39 15 , , , cord-329245-6tj2k1yn 39 16 we -PRON- PRP cord-329245-6tj2k1yn 39 17 found find VBD cord-329245-6tj2k1yn 39 18 that that IN cord-329245-6tj2k1yn 39 19 the the DT cord-329245-6tj2k1yn 39 20 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 39 21 tails tail NNS cord-329245-6tj2k1yn 39 22 of of IN cord-329245-6tj2k1yn 39 23 both both DT cord-329245-6tj2k1yn 39 24 proteins protein NNS cord-329245-6tj2k1yn 39 25 are be VBP cord-329245-6tj2k1yn 39 26 required require VBN cord-329245-6tj2k1yn 39 27 for for IN cord-329245-6tj2k1yn 39 28 their -PRON- PRP$ cord-329245-6tj2k1yn 39 29 interaction interaction NN cord-329245-6tj2k1yn 39 30 . . . cord-329245-6tj2k1yn 40 1 We -PRON- PRP cord-329245-6tj2k1yn 40 2 found find VBD cord-329245-6tj2k1yn 40 3 that that IN cord-329245-6tj2k1yn 40 4 IBV IBV NNP cord-329245-6tj2k1yn 40 5 E E NNP cord-329245-6tj2k1yn 40 6 and and CC cord-329245-6tj2k1yn 40 7 M M NNP cord-329245-6tj2k1yn 40 8 proteins protein NNS cord-329245-6tj2k1yn 40 9 can can MD cord-329245-6tj2k1yn 40 10 be be VB cord-329245-6tj2k1yn 40 11 efficiently efficiently RB cord-329245-6tj2k1yn 40 12 assembled assemble VBN cord-329245-6tj2k1yn 40 13 into into IN cord-329245-6tj2k1yn 40 14 VLPs vlp NNS cord-329245-6tj2k1yn 40 15 in in IN cord-329245-6tj2k1yn 40 16 OST7 OST7 NNP cord-329245-6tj2k1yn 40 17 - - HYPH cord-329245-6tj2k1yn 40 18 1 1 CD cord-329245-6tj2k1yn 40 19 cells cell NNS cord-329245-6tj2k1yn 40 20 , , , cord-329245-6tj2k1yn 40 21 and and CC cord-329245-6tj2k1yn 40 22 we -PRON- PRP cord-329245-6tj2k1yn 40 23 investigated investigate VBD cord-329245-6tj2k1yn 40 24 which which WDT cord-329245-6tj2k1yn 40 25 domains domain NNS cord-329245-6tj2k1yn 40 26 of of IN cord-329245-6tj2k1yn 40 27 IBV IBV NNP cord-329245-6tj2k1yn 40 28 E E NNP cord-329245-6tj2k1yn 40 29 and and CC cord-329245-6tj2k1yn 40 30 M M NNP cord-329245-6tj2k1yn 40 31 are be VBP cord-329245-6tj2k1yn 40 32 required require VBN cord-329245-6tj2k1yn 40 33 for for IN cord-329245-6tj2k1yn 40 34 this this DT cord-329245-6tj2k1yn 40 35 process process NN cord-329245-6tj2k1yn 40 36 . . . cord-329245-6tj2k1yn 41 1 Interestingly interestingly RB cord-329245-6tj2k1yn 41 2 , , , cord-329245-6tj2k1yn 41 3 the the DT cord-329245-6tj2k1yn 41 4 interaction interaction NN cord-329245-6tj2k1yn 41 5 of of IN cord-329245-6tj2k1yn 41 6 M M NNP cord-329245-6tj2k1yn 41 7 and and CC cord-329245-6tj2k1yn 41 8 E E NNP cord-329245-6tj2k1yn 41 9 , , , cord-329245-6tj2k1yn 41 10 as as IN cord-329245-6tj2k1yn 41 11 measured measure VBN cord-329245-6tj2k1yn 41 12 by by IN cord-329245-6tj2k1yn 41 13 crosslinking crosslinking NN cord-329245-6tj2k1yn 41 14 , , , cord-329245-6tj2k1yn 41 15 was be VBD cord-329245-6tj2k1yn 41 16 not not RB cord-329245-6tj2k1yn 41 17 sufficient sufficient JJ cord-329245-6tj2k1yn 41 18 for for IN cord-329245-6tj2k1yn 41 19 VLP VLP NNP cord-329245-6tj2k1yn 41 20 formation formation NN cord-329245-6tj2k1yn 41 21 . . . cord-329245-6tj2k1yn 42 1 It -PRON- PRP cord-329245-6tj2k1yn 42 2 is be VBZ cord-329245-6tj2k1yn 42 3 likely likely JJ cord-329245-6tj2k1yn 42 4 that that IN cord-329245-6tj2k1yn 42 5 interactions interaction NNS cord-329245-6tj2k1yn 42 6 of of IN cord-329245-6tj2k1yn 42 7 E E NNP cord-329245-6tj2k1yn 42 8 and and CC cord-329245-6tj2k1yn 42 9 M M NNP cord-329245-6tj2k1yn 42 10 with with IN cord-329245-6tj2k1yn 42 11 each each DT cord-329245-6tj2k1yn 42 12 other other JJ cord-329245-6tj2k1yn 42 13 as as RB cord-329245-6tj2k1yn 42 14 well well RB cord-329245-6tj2k1yn 42 15 as as IN cord-329245-6tj2k1yn 42 16 the the DT cord-329245-6tj2k1yn 42 17 membrane membrane NN cord-329245-6tj2k1yn 42 18 bilayer bilayer NN cord-329245-6tj2k1yn 42 19 are be VBP cord-329245-6tj2k1yn 42 20 important important JJ cord-329245-6tj2k1yn 42 21 for for IN cord-329245-6tj2k1yn 42 22 VLP VLP NNP cord-329245-6tj2k1yn 42 23 formation formation NN cord-329245-6tj2k1yn 42 24 and and CC cord-329245-6tj2k1yn 42 25 virus virus NN cord-329245-6tj2k1yn 42 26 budding bud VBG cord-329245-6tj2k1yn 42 27 . . . cord-329245-6tj2k1yn 43 1 Although although IN cord-329245-6tj2k1yn 43 2 the the DT cord-329245-6tj2k1yn 43 3 IBV IBV NNP cord-329245-6tj2k1yn 43 4 E E NNP cord-329245-6tj2k1yn 43 5 protein protein NN cord-329245-6tj2k1yn 43 6 is be VBZ cord-329245-6tj2k1yn 43 7 distributed distribute VBN cord-329245-6tj2k1yn 43 8 throughout throughout IN cord-329245-6tj2k1yn 43 9 the the DT cord-329245-6tj2k1yn 43 10 Golgi Golgi NNP cord-329245-6tj2k1yn 43 11 stack stack NN cord-329245-6tj2k1yn 43 12 when when WRB cord-329245-6tj2k1yn 43 13 expressed express VBD cord-329245-6tj2k1yn 43 14 alone alone JJ cord-329245-6tj2k1yn 43 15 Machamer Machamer NNP cord-329245-6tj2k1yn 43 16 , , , cord-329245-6tj2k1yn 43 17 2000 2000 CD cord-329245-6tj2k1yn 43 18 , , , cord-329245-6tj2k1yn 43 19 2001 2001 CD cord-329245-6tj2k1yn 43 20 ) ) -RRB- cord-329245-6tj2k1yn 43 21 , , , cord-329245-6tj2k1yn 43 22 the the DT cord-329245-6tj2k1yn 43 23 IBV IBV NNP cord-329245-6tj2k1yn 43 24 E E NNP cord-329245-6tj2k1yn 43 25 and and CC cord-329245-6tj2k1yn 43 26 M M NNP cord-329245-6tj2k1yn 43 27 proteins protein NNS cord-329245-6tj2k1yn 43 28 exactly exactly RB cord-329245-6tj2k1yn 43 29 colocalize colocalize VBP cord-329245-6tj2k1yn 43 30 in in IN cord-329245-6tj2k1yn 43 31 IBV IBV NNP cord-329245-6tj2k1yn 43 32 - - HYPH cord-329245-6tj2k1yn 43 33 infected infect VBN cord-329245-6tj2k1yn 43 34 Vero Vero NNP cord-329245-6tj2k1yn 43 35 cells cell NNS cord-329245-6tj2k1yn 43 36 examined examine VBN cord-329245-6tj2k1yn 43 37 by by IN cord-329245-6tj2k1yn 43 38 indirect indirect JJ cord-329245-6tj2k1yn 43 39 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 43 40 at at IN cord-329245-6tj2k1yn 43 41 early early JJ cord-329245-6tj2k1yn 43 42 times time NNS cord-329245-6tj2k1yn 43 43 postinfection postinfection NN cord-329245-6tj2k1yn 43 44 ( ( -LRB- cord-329245-6tj2k1yn 43 45 Fig Fig NNP cord-329245-6tj2k1yn 43 46 . . . cord-329245-6tj2k1yn 43 47 1A 1a NN cord-329245-6tj2k1yn 43 48 ) ) -RRB- cord-329245-6tj2k1yn 43 49 . . . cord-329245-6tj2k1yn 44 1 We -PRON- PRP cord-329245-6tj2k1yn 44 2 wanted want VBD cord-329245-6tj2k1yn 44 3 to to TO cord-329245-6tj2k1yn 44 4 investigate investigate VB cord-329245-6tj2k1yn 44 5 the the DT cord-329245-6tj2k1yn 44 6 possibility possibility NN cord-329245-6tj2k1yn 44 7 that that IN cord-329245-6tj2k1yn 44 8 this this DT cord-329245-6tj2k1yn 44 9 precise precise JJ cord-329245-6tj2k1yn 44 10 colocalization colocalization NN cord-329245-6tj2k1yn 44 11 was be VBD cord-329245-6tj2k1yn 44 12 indicative indicative JJ cord-329245-6tj2k1yn 44 13 of of IN cord-329245-6tj2k1yn 44 14 a a DT cord-329245-6tj2k1yn 44 15 physical physical JJ cord-329245-6tj2k1yn 44 16 interaction interaction NN cord-329245-6tj2k1yn 44 17 between between IN cord-329245-6tj2k1yn 44 18 the the DT cord-329245-6tj2k1yn 44 19 two two CD cord-329245-6tj2k1yn 44 20 proteins protein NNS cord-329245-6tj2k1yn 44 21 . . . cord-329245-6tj2k1yn 45 1 Since since IN cord-329245-6tj2k1yn 45 2 attempts attempt NNS cord-329245-6tj2k1yn 45 3 to to TO cord-329245-6tj2k1yn 45 4 coimmunoprecipitate coimmunoprecipitate VB cord-329245-6tj2k1yn 45 5 E E NNP cord-329245-6tj2k1yn 45 6 and and CC cord-329245-6tj2k1yn 45 7 M M NNP cord-329245-6tj2k1yn 45 8 proteins protein NNS cord-329245-6tj2k1yn 45 9 from from IN cord-329245-6tj2k1yn 45 10 IBV IBV NNP cord-329245-6tj2k1yn 45 11 - - HYPH cord-329245-6tj2k1yn 45 12 infected infect VBN cord-329245-6tj2k1yn 45 13 Vero Vero NNP cord-329245-6tj2k1yn 45 14 cells cell NNS cord-329245-6tj2k1yn 45 15 in in IN cord-329245-6tj2k1yn 45 16 a a DT cord-329245-6tj2k1yn 45 17 variety variety NN cord-329245-6tj2k1yn 45 18 of of IN cord-329245-6tj2k1yn 45 19 detergent detergent NN cord-329245-6tj2k1yn 45 20 conditions condition NNS cord-329245-6tj2k1yn 45 21 were be VBD cord-329245-6tj2k1yn 45 22 unsuccessful unsuccessful JJ cord-329245-6tj2k1yn 45 23 , , , cord-329245-6tj2k1yn 45 24 we -PRON- PRP cord-329245-6tj2k1yn 45 25 employed employ VBD cord-329245-6tj2k1yn 45 26 the the DT cord-329245-6tj2k1yn 45 27 cellpermeable cellpermeable JJ cord-329245-6tj2k1yn 45 28 chemical chemical NN cord-329245-6tj2k1yn 45 29 crosslinker crosslinker NNP cord-329245-6tj2k1yn 45 30 DSP DSP NNP cord-329245-6tj2k1yn 45 31 , , , cord-329245-6tj2k1yn 45 32 which which WDT cord-329245-6tj2k1yn 45 33 is be VBZ cord-329245-6tj2k1yn 45 34 a a DT cord-329245-6tj2k1yn 45 35 thiol thiol NN cord-329245-6tj2k1yn 45 36 - - HYPH cord-329245-6tj2k1yn 45 37 cleavable cleavable NN cord-329245-6tj2k1yn 45 38 molecule molecule NN cord-329245-6tj2k1yn 45 39 that that WDT cord-329245-6tj2k1yn 45 40 contains contain VBZ cord-329245-6tj2k1yn 45 41 two two CD cord-329245-6tj2k1yn 45 42 amine amine JJ cord-329245-6tj2k1yn 45 43 - - HYPH cord-329245-6tj2k1yn 45 44 reactive reactive JJ cord-329245-6tj2k1yn 45 45 groups group NNS cord-329245-6tj2k1yn 45 46 separated separate VBN cord-329245-6tj2k1yn 45 47 by by IN cord-329245-6tj2k1yn 45 48 a a DT cord-329245-6tj2k1yn 45 49 12 12 CD cord-329245-6tj2k1yn 45 50 Å å NN cord-329245-6tj2k1yn 45 51 spacer spacer NN cord-329245-6tj2k1yn 45 52 arm arm NN cord-329245-6tj2k1yn 45 53 . . . cord-329245-6tj2k1yn 46 1 IBV IBV NNP cord-329245-6tj2k1yn 46 2 - - HYPH cord-329245-6tj2k1yn 46 3 infected infect VBN cord-329245-6tj2k1yn 46 4 Vero Vero NNP cord-329245-6tj2k1yn 46 5 cells cell NNS cord-329245-6tj2k1yn 46 6 were be VBD cord-329245-6tj2k1yn 46 7 radiolabeled radiolabele VBN cord-329245-6tj2k1yn 46 8 at at IN cord-329245-6tj2k1yn 46 9 45 45 CD cord-329245-6tj2k1yn 46 10 h h NN cord-329245-6tj2k1yn 46 11 postinfection postinfection NN cord-329245-6tj2k1yn 46 12 and and CC cord-329245-6tj2k1yn 46 13 treated treat VBN cord-329245-6tj2k1yn 46 14 with with IN cord-329245-6tj2k1yn 46 15 DSP DSP NNP cord-329245-6tj2k1yn 46 16 prior prior RB cord-329245-6tj2k1yn 46 17 to to IN cord-329245-6tj2k1yn 46 18 lysis lysis NN cord-329245-6tj2k1yn 46 19 and and CC cord-329245-6tj2k1yn 46 20 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 46 21 with with IN cord-329245-6tj2k1yn 46 22 anti anti JJ cord-329245-6tj2k1yn 46 23 - - JJ cord-329245-6tj2k1yn 46 24 E e JJ cord-329245-6tj2k1yn 46 25 or or CC cord-329245-6tj2k1yn 46 26 anti anti JJ cord-329245-6tj2k1yn 46 27 - - JJ cord-329245-6tj2k1yn 46 28 M m JJ cord-329245-6tj2k1yn 46 29 antibodies antibody NNS cord-329245-6tj2k1yn 46 30 . . . cord-329245-6tj2k1yn 47 1 When when WRB cord-329245-6tj2k1yn 47 2 we -PRON- PRP cord-329245-6tj2k1yn 47 3 analyzed analyze VBD cord-329245-6tj2k1yn 47 4 the the DT cord-329245-6tj2k1yn 47 5 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 47 6 by by IN cord-329245-6tj2k1yn 47 7 SDS SDS NNP cord-329245-6tj2k1yn 47 8 - - HYPH cord-329245-6tj2k1yn 47 9 PAGE PAGE NNP cord-329245-6tj2k1yn 47 10 , , , cord-329245-6tj2k1yn 47 11 we -PRON- PRP cord-329245-6tj2k1yn 47 12 found find VBD cord-329245-6tj2k1yn 47 13 that that IN cord-329245-6tj2k1yn 47 14 M M NNP cord-329245-6tj2k1yn 47 15 was be VBD cord-329245-6tj2k1yn 47 16 present present JJ cord-329245-6tj2k1yn 47 17 in in IN cord-329245-6tj2k1yn 47 18 the the DT cord-329245-6tj2k1yn 47 19 anti anti JJ cord-329245-6tj2k1yn 47 20 - - JJ cord-329245-6tj2k1yn 47 21 E e JJ cord-329245-6tj2k1yn 47 22 immunoprecipitations immunoprecipitation NNS cord-329245-6tj2k1yn 47 23 and and CC cord-329245-6tj2k1yn 47 24 vice vice NN cord-329245-6tj2k1yn 47 25 versa versa RB cord-329245-6tj2k1yn 48 1 ( ( -LRB- cord-329245-6tj2k1yn 48 2 Fig Fig NNP cord-329245-6tj2k1yn 48 3 . . . cord-329245-6tj2k1yn 48 4 1B 1b CD cord-329245-6tj2k1yn 48 5 , , , cord-329245-6tj2k1yn 48 6 lanes lane NNS cord-329245-6tj2k1yn 48 7 _SP cord-329245-6tj2k1yn 48 8 4 4 CD cord-329245-6tj2k1yn 48 9 and and CC cord-329245-6tj2k1yn 48 10 8) 8) NN cord-329245-6tj2k1yn 48 11 . . . cord-329245-6tj2k1yn 49 1 This this DT cord-329245-6tj2k1yn 49 2 association association NN cord-329245-6tj2k1yn 49 3 was be VBD cord-329245-6tj2k1yn 49 4 dependent dependent JJ cord-329245-6tj2k1yn 49 5 on on IN cord-329245-6tj2k1yn 49 6 the the DT cord-329245-6tj2k1yn 49 7 presence presence NN cord-329245-6tj2k1yn 49 8 of of IN cord-329245-6tj2k1yn 49 9 DSP DSP NNP cord-329245-6tj2k1yn 49 10 since since IN cord-329245-6tj2k1yn 49 11 it -PRON- PRP cord-329245-6tj2k1yn 49 12 was be VBD cord-329245-6tj2k1yn 49 13 not not RB cord-329245-6tj2k1yn 49 14 seen see VBN cord-329245-6tj2k1yn 49 15 in in IN cord-329245-6tj2k1yn 49 16 samples sample NNS cord-329245-6tj2k1yn 49 17 that that WDT cord-329245-6tj2k1yn 49 18 were be VBD cord-329245-6tj2k1yn 49 19 mock mock JJ cord-329245-6tj2k1yn 49 20 - - HYPH cord-329245-6tj2k1yn 49 21 treated treat VBN cord-329245-6tj2k1yn 49 22 ( ( -LRB- cord-329245-6tj2k1yn 49 23 lanes lane NNS cord-329245-6tj2k1yn 49 24 2 2 CD cord-329245-6tj2k1yn 49 25 and and CC cord-329245-6tj2k1yn 49 26 6 6 CD cord-329245-6tj2k1yn 49 27 ) ) -RRB- cord-329245-6tj2k1yn 49 28 . . . cord-329245-6tj2k1yn 50 1 Immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 50 2 with with IN cord-329245-6tj2k1yn 50 3 irrelevant irrelevant JJ cord-329245-6tj2k1yn 50 4 antibodies antibody NNS cord-329245-6tj2k1yn 50 5 yielded yield VBD cord-329245-6tj2k1yn 50 6 no no DT cord-329245-6tj2k1yn 50 7 visible visible JJ cord-329245-6tj2k1yn 50 8 bands band NNS cord-329245-6tj2k1yn 50 9 ( ( -LRB- cord-329245-6tj2k1yn 50 10 not not RB cord-329245-6tj2k1yn 50 11 shown show VBN cord-329245-6tj2k1yn 50 12 ) ) -RRB- cord-329245-6tj2k1yn 50 13 . . . cord-329245-6tj2k1yn 51 1 In in IN cord-329245-6tj2k1yn 51 2 the the DT cord-329245-6tj2k1yn 51 3 absence absence NN cord-329245-6tj2k1yn 51 4 of of IN cord-329245-6tj2k1yn 51 5 ␤ ␤ NNP cord-329245-6tj2k1yn 51 6 -mercaptoethanol -mercaptoethanol NNP cord-329245-6tj2k1yn 51 7 , , , cord-329245-6tj2k1yn 51 8 which which WDT cord-329245-6tj2k1yn 51 9 cleaves cleave VBZ cord-329245-6tj2k1yn 51 10 the the DT cord-329245-6tj2k1yn 51 11 disulfide disulfide JJ cord-329245-6tj2k1yn 51 12 bond bond NN cord-329245-6tj2k1yn 51 13 of of IN cord-329245-6tj2k1yn 51 14 DSP DSP NNP cord-329245-6tj2k1yn 51 15 , , , cord-329245-6tj2k1yn 51 16 the the DT cord-329245-6tj2k1yn 51 17 unseparated unseparated JJ cord-329245-6tj2k1yn 51 18 crosslinked crosslinked JJ cord-329245-6tj2k1yn 51 19 E E NNP cord-329245-6tj2k1yn 51 20 and and CC cord-329245-6tj2k1yn 51 21 M M NNP cord-329245-6tj2k1yn 51 22 proteins protein NNS cord-329245-6tj2k1yn 51 23 were be VBD cord-329245-6tj2k1yn 51 24 present present JJ cord-329245-6tj2k1yn 51 25 as as IN cord-329245-6tj2k1yn 51 26 high high JJ cord-329245-6tj2k1yn 51 27 molecular molecular JJ cord-329245-6tj2k1yn 51 28 weight weight NN cord-329245-6tj2k1yn 51 29 aggregates aggregate NNS cord-329245-6tj2k1yn 51 30 and and CC cord-329245-6tj2k1yn 51 31 migrated migrate VBD cord-329245-6tj2k1yn 51 32 at at IN cord-329245-6tj2k1yn 51 33 a a DT cord-329245-6tj2k1yn 51 34 high high JJ cord-329245-6tj2k1yn 51 35 position position NN cord-329245-6tj2k1yn 51 36 on on IN cord-329245-6tj2k1yn 51 37 the the DT cord-329245-6tj2k1yn 51 38 gel gel NN cord-329245-6tj2k1yn 51 39 ( ( -LRB- cord-329245-6tj2k1yn 51 40 not not RB cord-329245-6tj2k1yn 51 41 shown show VBN cord-329245-6tj2k1yn 51 42 ) ) -RRB- cord-329245-6tj2k1yn 51 43 . . . cord-329245-6tj2k1yn 52 1 The the DT cord-329245-6tj2k1yn 52 2 crosslinking crosslinking NN cord-329245-6tj2k1yn 52 3 and and CC cord-329245-6tj2k1yn 52 4 coimmunoprecipitation coimmunoprecipitation NN cord-329245-6tj2k1yn 52 5 was be VBD cord-329245-6tj2k1yn 52 6 not not RB cord-329245-6tj2k1yn 52 7 quantitative quantitative JJ cord-329245-6tj2k1yn 52 8 , , , cord-329245-6tj2k1yn 52 9 since since IN cord-329245-6tj2k1yn 52 10 only only RB cord-329245-6tj2k1yn 52 11 a a DT cord-329245-6tj2k1yn 52 12 fraction fraction NN cord-329245-6tj2k1yn 52 13 of of IN cord-329245-6tj2k1yn 52 14 the the DT cord-329245-6tj2k1yn 52 15 total total JJ cord-329245-6tj2k1yn 52 16 E e NN cord-329245-6tj2k1yn 52 17 protein protein NN cord-329245-6tj2k1yn 52 18 ( ( -LRB- cord-329245-6tj2k1yn 52 19 as as IN cord-329245-6tj2k1yn 52 20 measured measure VBN cord-329245-6tj2k1yn 52 21 by by IN cord-329245-6tj2k1yn 52 22 the the DT cord-329245-6tj2k1yn 52 23 amount amount NN cord-329245-6tj2k1yn 52 24 of of IN cord-329245-6tj2k1yn 52 25 E e NN cord-329245-6tj2k1yn 52 26 in in IN cord-329245-6tj2k1yn 52 27 the the DT cord-329245-6tj2k1yn 52 28 anti anti JJ cord-329245-6tj2k1yn 52 29 - - JJ cord-329245-6tj2k1yn 52 30 E e JJ cord-329245-6tj2k1yn 52 31 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 52 32 ) ) -RRB- cord-329245-6tj2k1yn 52 33 was be VBD cord-329245-6tj2k1yn 52 34 found find VBN cord-329245-6tj2k1yn 52 35 in in IN cord-329245-6tj2k1yn 52 36 the the DT cord-329245-6tj2k1yn 52 37 anti anti JJ cord-329245-6tj2k1yn 52 38 - - JJ cord-329245-6tj2k1yn 52 39 M m JJ cord-329245-6tj2k1yn 52 40 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 52 41 . . . cord-329245-6tj2k1yn 53 1 This this DT cord-329245-6tj2k1yn 53 2 could could MD cord-329245-6tj2k1yn 53 3 be be VB cord-329245-6tj2k1yn 53 4 due due JJ cord-329245-6tj2k1yn 53 5 to to IN cord-329245-6tj2k1yn 53 6 the the DT cord-329245-6tj2k1yn 53 7 inherent inherent JJ cord-329245-6tj2k1yn 53 8 inefficiency inefficiency NN cord-329245-6tj2k1yn 53 9 of of IN cord-329245-6tj2k1yn 53 10 chemical chemical NN cord-329245-6tj2k1yn 53 11 crosslinking crosslinking NN cord-329245-6tj2k1yn 53 12 . . . cord-329245-6tj2k1yn 54 1 Alternatively alternatively RB cord-329245-6tj2k1yn 54 2 , , , cord-329245-6tj2k1yn 54 3 this this DT cord-329245-6tj2k1yn 54 4 result result NN cord-329245-6tj2k1yn 54 5 could could MD cord-329245-6tj2k1yn 54 6 reflect reflect VB cord-329245-6tj2k1yn 54 7 the the DT cord-329245-6tj2k1yn 54 8 small small JJ cord-329245-6tj2k1yn 54 9 E e NN cord-329245-6tj2k1yn 54 10 : : : cord-329245-6tj2k1yn 54 11 M m NN cord-329245-6tj2k1yn 54 12 ratio ratio NN cord-329245-6tj2k1yn 54 13 in in IN cord-329245-6tj2k1yn 54 14 virions virion NNS cord-329245-6tj2k1yn 54 15 ( ( -LRB- cord-329245-6tj2k1yn 54 16 Liu Liu NNP cord-329245-6tj2k1yn 54 17 Fig Fig NNP cord-329245-6tj2k1yn 54 18 . . . cord-329245-6tj2k1yn 55 1 1 1 LS cord-329245-6tj2k1yn 55 2 . . . cord-329245-6tj2k1yn 56 1 IBV IBV NNP cord-329245-6tj2k1yn 56 2 E E NNP cord-329245-6tj2k1yn 56 3 and and CC cord-329245-6tj2k1yn 56 4 M M NNP cord-329245-6tj2k1yn 56 5 colocalize colocalize NN cord-329245-6tj2k1yn 56 6 and and CC cord-329245-6tj2k1yn 56 7 interact interact VB cord-329245-6tj2k1yn 56 8 in in IN cord-329245-6tj2k1yn 56 9 IBV IBV NNP cord-329245-6tj2k1yn 56 10 - - HYPH cord-329245-6tj2k1yn 56 11 infected infect VBN cord-329245-6tj2k1yn 56 12 Vero Vero NNP cord-329245-6tj2k1yn 56 13 cells cell NNS cord-329245-6tj2k1yn 56 14 . . . cord-329245-6tj2k1yn 57 1 ( ( -LRB- cord-329245-6tj2k1yn 57 2 A a DT cord-329245-6tj2k1yn 57 3 ) ) -RRB- cord-329245-6tj2k1yn 57 4 IBV IBV NNP cord-329245-6tj2k1yn 57 5 - - HYPH cord-329245-6tj2k1yn 57 6 infected infect VBN cord-329245-6tj2k1yn 57 7 Vero Vero NNP cord-329245-6tj2k1yn 57 8 cells cell NNS cord-329245-6tj2k1yn 57 9 were be VBD cord-329245-6tj2k1yn 57 10 fixed fix VBN cord-329245-6tj2k1yn 57 11 for for IN cord-329245-6tj2k1yn 57 12 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 57 13 at at IN cord-329245-6tj2k1yn 57 14 6 6 CD cord-329245-6tj2k1yn 57 15 h h NN cord-329245-6tj2k1yn 57 16 postinfection postinfection NN cord-329245-6tj2k1yn 57 17 and and CC cord-329245-6tj2k1yn 57 18 double double JJ cord-329245-6tj2k1yn 57 19 labeled label VBN cord-329245-6tj2k1yn 57 20 with with IN cord-329245-6tj2k1yn 57 21 rat rat NN cord-329245-6tj2k1yn 57 22 anti anti JJ cord-329245-6tj2k1yn 57 23 - - JJ cord-329245-6tj2k1yn 57 24 E e JJ cord-329245-6tj2k1yn 57 25 antibody antibody NN cord-329245-6tj2k1yn 57 26 ( ( -LRB- cord-329245-6tj2k1yn 57 27 a a LS cord-329245-6tj2k1yn 57 28 ) ) -RRB- cord-329245-6tj2k1yn 57 29 and and CC cord-329245-6tj2k1yn 57 30 rabbit rabbit NN cord-329245-6tj2k1yn 57 31 anti anti NNP cord-329245-6tj2k1yn 57 32 - - JJ cord-329245-6tj2k1yn 57 33 M M NNP cord-329245-6tj2k1yn 57 34 antibody antibody NN cord-329245-6tj2k1yn 57 35 ( ( -LRB- cord-329245-6tj2k1yn 57 36 b b NN cord-329245-6tj2k1yn 57 37 ) ) -RRB- cord-329245-6tj2k1yn 57 38 . . . cord-329245-6tj2k1yn 58 1 Secondary secondary JJ cord-329245-6tj2k1yn 58 2 antibodies antibody NNS cord-329245-6tj2k1yn 58 3 were be VBD cord-329245-6tj2k1yn 58 4 fluorescein fluorescein NN cord-329245-6tj2k1yn 58 5 - - HYPH cord-329245-6tj2k1yn 58 6 conjugated conjugate VBN cord-329245-6tj2k1yn 58 7 goat goat NN cord-329245-6tj2k1yn 58 8 antirat antirat NNP cord-329245-6tj2k1yn 58 9 IgG IgG NNP cord-329245-6tj2k1yn 58 10 and and CC cord-329245-6tj2k1yn 58 11 Texas Texas NNP cord-329245-6tj2k1yn 58 12 red red JJ cord-329245-6tj2k1yn 58 13 conjugated conjugated JJ cord-329245-6tj2k1yn 58 14 donkey donkey NNP cord-329245-6tj2k1yn 58 15 anti anti JJ cord-329245-6tj2k1yn 58 16 - - JJ cord-329245-6tj2k1yn 58 17 rabbit rabbit JJ cord-329245-6tj2k1yn 59 1 IgG. IgG. NNP cord-329245-6tj2k1yn 59 2 Panel Panel NNP cord-329245-6tj2k1yn 59 3 c c NN cord-329245-6tj2k1yn 59 4 is be VBZ cord-329245-6tj2k1yn 59 5 a a DT cord-329245-6tj2k1yn 59 6 differential differential JJ cord-329245-6tj2k1yn 59 7 interference interference NN cord-329245-6tj2k1yn 59 8 contrast contrast NN cord-329245-6tj2k1yn 59 9 ( ( -LRB- cord-329245-6tj2k1yn 59 10 DIC DIC NNP cord-329245-6tj2k1yn 59 11 ) ) -RRB- cord-329245-6tj2k1yn 59 12 image image NN cord-329245-6tj2k1yn 59 13 of of IN cord-329245-6tj2k1yn 59 14 the the DT cord-329245-6tj2k1yn 59 15 labeled label VBN cord-329245-6tj2k1yn 59 16 cells cell NNS cord-329245-6tj2k1yn 59 17 . . . cord-329245-6tj2k1yn 60 1 Bar bar NN cord-329245-6tj2k1yn 60 2 , , , cord-329245-6tj2k1yn 60 3 15 15 CD cord-329245-6tj2k1yn 60 4 m. m. NN cord-329245-6tj2k1yn 60 5 ( ( -LRB- cord-329245-6tj2k1yn 60 6 B B NNP cord-329245-6tj2k1yn 60 7 ) ) -RRB- cord-329245-6tj2k1yn 60 8 IBV IBV NNP cord-329245-6tj2k1yn 60 9 - - HYPH cord-329245-6tj2k1yn 60 10 infected infect VBN cord-329245-6tj2k1yn 60 11 Vero Vero NNP cord-329245-6tj2k1yn 60 12 cells cell NNS cord-329245-6tj2k1yn 60 13 were be VBD cord-329245-6tj2k1yn 60 14 labeled label VBN cord-329245-6tj2k1yn 60 15 with with IN cord-329245-6tj2k1yn 60 16 [ [ -LRB- cord-329245-6tj2k1yn 60 17 35 35 CD cord-329245-6tj2k1yn 60 18 S]methionine S]methionine NNP cord-329245-6tj2k1yn 60 19 - - HYPH cord-329245-6tj2k1yn 60 20 cysteine cysteine NN cord-329245-6tj2k1yn 60 21 at at IN cord-329245-6tj2k1yn 60 22 45 45 CD cord-329245-6tj2k1yn 60 23 h h NN cord-329245-6tj2k1yn 60 24 postinfection postinfection NN cord-329245-6tj2k1yn 60 25 , , , cord-329245-6tj2k1yn 60 26 treated treat VBN cord-329245-6tj2k1yn 60 27 with with IN cord-329245-6tj2k1yn 60 28 DSP DSP NNP cord-329245-6tj2k1yn 60 29 as as IN cord-329245-6tj2k1yn 60 30 indicated indicate VBN cord-329245-6tj2k1yn 60 31 , , , cord-329245-6tj2k1yn 60 32 lysed lyse VBN cord-329245-6tj2k1yn 60 33 , , , cord-329245-6tj2k1yn 60 34 and and CC cord-329245-6tj2k1yn 60 35 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 60 36 with with IN cord-329245-6tj2k1yn 60 37 anti anti JJ cord-329245-6tj2k1yn 60 38 - - JJ cord-329245-6tj2k1yn 60 39 E e JJ cord-329245-6tj2k1yn 60 40 or or CC cord-329245-6tj2k1yn 60 41 anti anti JJ cord-329245-6tj2k1yn 60 42 - - JJ cord-329245-6tj2k1yn 60 43 M m JJ cord-329245-6tj2k1yn 60 44 antibodies antibody NNS cord-329245-6tj2k1yn 60 45 as as IN cord-329245-6tj2k1yn 60 46 described describe VBN cord-329245-6tj2k1yn 60 47 under under IN cord-329245-6tj2k1yn 60 48 Materials material NNS cord-329245-6tj2k1yn 60 49 and and CC cord-329245-6tj2k1yn 60 50 methods method NNS cord-329245-6tj2k1yn 60 51 . . . cord-329245-6tj2k1yn 61 1 The the DT cord-329245-6tj2k1yn 61 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 61 3 were be VBD cord-329245-6tj2k1yn 61 4 analyzed analyze VBN cord-329245-6tj2k1yn 61 5 by by IN cord-329245-6tj2k1yn 61 6 SDS-15 sds-15 CD cord-329245-6tj2k1yn 61 7 % % NN cord-329245-6tj2k1yn 61 8 PAGE page NN cord-329245-6tj2k1yn 61 9 , , , cord-329245-6tj2k1yn 61 10 in in IN cord-329245-6tj2k1yn 61 11 the the DT cord-329245-6tj2k1yn 61 12 presence presence NN cord-329245-6tj2k1yn 61 13 or or CC cord-329245-6tj2k1yn 61 14 absence absence NN cord-329245-6tj2k1yn 61 15 of of IN cord-329245-6tj2k1yn 61 16 ␤ ␤ . cord-329245-6tj2k1yn 62 1 -mercaptoethanol -mercaptoethanol : cord-329245-6tj2k1yn 62 2 ( ( -LRB- cord-329245-6tj2k1yn 62 3 ␤ ␤ `` cord-329245-6tj2k1yn 62 4 ME ME NNP cord-329245-6tj2k1yn 62 5 ) ) -RRB- cord-329245-6tj2k1yn 62 6 as as IN cord-329245-6tj2k1yn 62 7 indicated indicate VBN cord-329245-6tj2k1yn 62 8 , , , cord-329245-6tj2k1yn 62 9 and and CC cord-329245-6tj2k1yn 62 10 fluorography fluorography NN cord-329245-6tj2k1yn 62 11 . . . cord-329245-6tj2k1yn 63 1 IBV IBV NNP cord-329245-6tj2k1yn 63 2 E E NNP cord-329245-6tj2k1yn 63 3 is be VBZ cord-329245-6tj2k1yn 63 4 coprecipitated coprecipitate VBN cord-329245-6tj2k1yn 63 5 with with IN cord-329245-6tj2k1yn 63 6 IBV IBV NNP cord-329245-6tj2k1yn 63 7 M M NNP cord-329245-6tj2k1yn 63 8 after after IN cord-329245-6tj2k1yn 63 9 crosslinking crosslinking NN cord-329245-6tj2k1yn 63 10 and and CC cord-329245-6tj2k1yn 63 11 vice vice NN cord-329245-6tj2k1yn 63 12 versa versa RB cord-329245-6tj2k1yn 63 13 . . . cord-329245-6tj2k1yn 64 1 These these DT cord-329245-6tj2k1yn 64 2 data datum NNS cord-329245-6tj2k1yn 64 3 are be VBP cord-329245-6tj2k1yn 64 4 representative representative JJ cord-329245-6tj2k1yn 64 5 of of IN cord-329245-6tj2k1yn 64 6 at at RB cord-329245-6tj2k1yn 64 7 least least RBS cord-329245-6tj2k1yn 64 8 three three CD cord-329245-6tj2k1yn 64 9 independent independent JJ cord-329245-6tj2k1yn 64 10 experiments experiment NNS cord-329245-6tj2k1yn 64 11 . . . cord-329245-6tj2k1yn 65 1 and and CC cord-329245-6tj2k1yn 65 2 Inglis Inglis NNP cord-329245-6tj2k1yn 65 3 , , , cord-329245-6tj2k1yn 65 4 1991 1991 CD cord-329245-6tj2k1yn 65 5 ) ) -RRB- cord-329245-6tj2k1yn 65 6 , , , cord-329245-6tj2k1yn 65 7 which which WDT cord-329245-6tj2k1yn 65 8 is be VBZ cord-329245-6tj2k1yn 65 9 presumably presumably RB cord-329245-6tj2k1yn 65 10 determined determine VBN cord-329245-6tj2k1yn 65 11 by by IN cord-329245-6tj2k1yn 65 12 the the DT cord-329245-6tj2k1yn 65 13 stoichiometry stoichiometry NN cord-329245-6tj2k1yn 65 14 of of IN cord-329245-6tj2k1yn 65 15 envelope envelope NN cord-329245-6tj2k1yn 65 16 protein protein NN cord-329245-6tj2k1yn 65 17 interactions interaction NNS cord-329245-6tj2k1yn 65 18 . . . cord-329245-6tj2k1yn 66 1 We -PRON- PRP cord-329245-6tj2k1yn 66 2 conclude conclude VBP cord-329245-6tj2k1yn 66 3 that that IN cord-329245-6tj2k1yn 66 4 the the DT cord-329245-6tj2k1yn 66 5 IBV IBV NNP cord-329245-6tj2k1yn 66 6 E E NNP cord-329245-6tj2k1yn 66 7 and and CC cord-329245-6tj2k1yn 66 8 M M NNP cord-329245-6tj2k1yn 66 9 proteins protein NNS cord-329245-6tj2k1yn 66 10 interact interact VBP cord-329245-6tj2k1yn 66 11 in in IN cord-329245-6tj2k1yn 66 12 infected infected JJ cord-329245-6tj2k1yn 66 13 cells cell NNS cord-329245-6tj2k1yn 66 14 . . . cord-329245-6tj2k1yn 67 1 IBV IBV NNP cord-329245-6tj2k1yn 67 2 - - HYPH cord-329245-6tj2k1yn 67 3 infected infect VBN cord-329245-6tj2k1yn 67 4 cells cell NNS cord-329245-6tj2k1yn 67 5 contain contain VBP cord-329245-6tj2k1yn 67 6 other other JJ cord-329245-6tj2k1yn 67 7 viral viral JJ cord-329245-6tj2k1yn 67 8 proteins protein NNS cord-329245-6tj2k1yn 67 9 which which WDT cord-329245-6tj2k1yn 67 10 might may MD cord-329245-6tj2k1yn 67 11 be be VB cord-329245-6tj2k1yn 67 12 required require VBN cord-329245-6tj2k1yn 67 13 for for IN cord-329245-6tj2k1yn 67 14 interaction interaction NN cord-329245-6tj2k1yn 67 15 of of IN cord-329245-6tj2k1yn 67 16 E E NNP cord-329245-6tj2k1yn 67 17 and and CC cord-329245-6tj2k1yn 67 18 M. M. NNP cord-329245-6tj2k1yn 67 19 To to TO cord-329245-6tj2k1yn 67 20 determine determine VB cord-329245-6tj2k1yn 67 21 if if IN cord-329245-6tj2k1yn 67 22 the the DT cord-329245-6tj2k1yn 67 23 IBV IBV NNP cord-329245-6tj2k1yn 67 24 E E NNP cord-329245-6tj2k1yn 67 25 and and CC cord-329245-6tj2k1yn 67 26 M M NNP cord-329245-6tj2k1yn 67 27 proteins protein NNS cord-329245-6tj2k1yn 67 28 could could MD cord-329245-6tj2k1yn 67 29 interact interact VB cord-329245-6tj2k1yn 67 30 directly directly RB cord-329245-6tj2k1yn 67 31 , , , cord-329245-6tj2k1yn 67 32 we -PRON- PRP cord-329245-6tj2k1yn 67 33 coexpressed coexpresse VBD cord-329245-6tj2k1yn 67 34 them -PRON- PRP cord-329245-6tj2k1yn 67 35 in in IN cord-329245-6tj2k1yn 67 36 OST7 OST7 NNP cord-329245-6tj2k1yn 67 37 - - HYPH cord-329245-6tj2k1yn 67 38 1 1 CD cord-329245-6tj2k1yn 67 39 cells cell NNS cord-329245-6tj2k1yn 67 40 . . . cord-329245-6tj2k1yn 68 1 We -PRON- PRP cord-329245-6tj2k1yn 68 2 used use VBD cord-329245-6tj2k1yn 68 3 indirect indirect JJ cord-329245-6tj2k1yn 68 4 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 68 5 to to TO cord-329245-6tj2k1yn 68 6 verify verify VB cord-329245-6tj2k1yn 68 7 coexpression coexpression NN cord-329245-6tj2k1yn 68 8 and and CC cord-329245-6tj2k1yn 68 9 correct correct VB cord-329245-6tj2k1yn 68 10 intracellular intracellular JJ cord-329245-6tj2k1yn 68 11 localization localization NN cord-329245-6tj2k1yn 68 12 of of IN cord-329245-6tj2k1yn 68 13 the the DT cord-329245-6tj2k1yn 68 14 two two CD cord-329245-6tj2k1yn 68 15 proteins protein NNS cord-329245-6tj2k1yn 68 16 ( ( -LRB- cord-329245-6tj2k1yn 68 17 Fig Fig NNP cord-329245-6tj2k1yn 68 18 . . . cord-329245-6tj2k1yn 68 19 3A 3A NNP cord-329245-6tj2k1yn 68 20 , , , cord-329245-6tj2k1yn 68 21 panels panel VBZ cord-329245-6tj2k1yn 68 22 a a DT cord-329245-6tj2k1yn 68 23 - - HYPH cord-329245-6tj2k1yn 68 24 c c NN cord-329245-6tj2k1yn 68 25 ) ) -RRB- cord-329245-6tj2k1yn 68 26 . . . cord-329245-6tj2k1yn 69 1 As as IN cord-329245-6tj2k1yn 69 2 expected expect VBN cord-329245-6tj2k1yn 69 3 , , , cord-329245-6tj2k1yn 69 4 the the DT cord-329245-6tj2k1yn 69 5 IBV IBV NNP cord-329245-6tj2k1yn 69 6 E E NNP cord-329245-6tj2k1yn 69 7 and and CC cord-329245-6tj2k1yn 69 8 M M NNP cord-329245-6tj2k1yn 69 9 proteins protein NNS cord-329245-6tj2k1yn 69 10 colocalized colocalize VBN cord-329245-6tj2k1yn 69 11 in in IN cord-329245-6tj2k1yn 69 12 a a DT cord-329245-6tj2k1yn 69 13 compact compact JJ cord-329245-6tj2k1yn 69 14 perinuclear perinuclear NNP cord-329245-6tj2k1yn 69 15 region region NN cord-329245-6tj2k1yn 69 16 characteristic characteristic NN cord-329245-6tj2k1yn 69 17 of of IN cord-329245-6tj2k1yn 69 18 the the DT cord-329245-6tj2k1yn 69 19 Golgi Golgi NNP cord-329245-6tj2k1yn 69 20 complex complex NN cord-329245-6tj2k1yn 69 21 in in IN cord-329245-6tj2k1yn 69 22 this this DT cord-329245-6tj2k1yn 69 23 cell cell NN cord-329245-6tj2k1yn 69 24 type type NN cord-329245-6tj2k1yn 69 25 , , , cord-329245-6tj2k1yn 69 26 as as IN cord-329245-6tj2k1yn 69 27 seen see VBN cord-329245-6tj2k1yn 69 28 previously previously RB cord-329245-6tj2k1yn 69 29 in in IN cord-329245-6tj2k1yn 69 30 BHK-21 BHK-21 NNP cord-329245-6tj2k1yn 69 31 cells cell NNS cord-329245-6tj2k1yn 69 32 ( ( -LRB- cord-329245-6tj2k1yn 69 33 Corse Corse NNP cord-329245-6tj2k1yn 69 34 and and CC cord-329245-6tj2k1yn 69 35 Machamer Machamer NNP cord-329245-6tj2k1yn 69 36 , , , cord-329245-6tj2k1yn 69 37 2000 2000 CD cord-329245-6tj2k1yn 69 38 ) ) -RRB- cord-329245-6tj2k1yn 69 39 . . . cord-329245-6tj2k1yn 70 1 When when WRB cord-329245-6tj2k1yn 70 2 we -PRON- PRP cord-329245-6tj2k1yn 70 3 treated treat VBD cord-329245-6tj2k1yn 70 4 OST7 OST7 NNP cord-329245-6tj2k1yn 70 5 - - HYPH cord-329245-6tj2k1yn 70 6 1 1 CD cord-329245-6tj2k1yn 70 7 cells cell NNS cord-329245-6tj2k1yn 70 8 coexpressing coexpresse VBG cord-329245-6tj2k1yn 70 9 IBV IBV NNP cord-329245-6tj2k1yn 70 10 E E NNP cord-329245-6tj2k1yn 70 11 and and CC cord-329245-6tj2k1yn 70 12 M M NNP cord-329245-6tj2k1yn 70 13 with with IN cord-329245-6tj2k1yn 70 14 DSP DSP NNP cord-329245-6tj2k1yn 70 15 and and CC cord-329245-6tj2k1yn 70 16 analyzed analyze VBD cord-329245-6tj2k1yn 70 17 anti anti JJ cord-329245-6tj2k1yn 70 18 - - JJ cord-329245-6tj2k1yn 70 19 E e JJ cord-329245-6tj2k1yn 70 20 and and CC cord-329245-6tj2k1yn 70 21 anti anti JJ cord-329245-6tj2k1yn 70 22 - - JJ cord-329245-6tj2k1yn 70 23 M m JJ cord-329245-6tj2k1yn 70 24 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 70 25 as as IN cord-329245-6tj2k1yn 70 26 described describe VBN cord-329245-6tj2k1yn 70 27 above above RB cord-329245-6tj2k1yn 70 28 , , , cord-329245-6tj2k1yn 70 29 we -PRON- PRP cord-329245-6tj2k1yn 70 30 found find VBD cord-329245-6tj2k1yn 70 31 that that IN cord-329245-6tj2k1yn 70 32 M M NNP cord-329245-6tj2k1yn 70 33 protein protein NN cord-329245-6tj2k1yn 70 34 was be VBD cord-329245-6tj2k1yn 70 35 present present JJ cord-329245-6tj2k1yn 70 36 in in IN cord-329245-6tj2k1yn 70 37 the the DT cord-329245-6tj2k1yn 70 38 anti anti JJ cord-329245-6tj2k1yn 70 39 - - JJ cord-329245-6tj2k1yn 70 40 E e JJ cord-329245-6tj2k1yn 70 41 immunoprecipitate immunoprecipitate NN cord-329245-6tj2k1yn 70 42 ( ( -LRB- cord-329245-6tj2k1yn 70 43 Fig Fig NNP cord-329245-6tj2k1yn 70 44 . . NNP cord-329245-6tj2k1yn 70 45 2 2 CD cord-329245-6tj2k1yn 70 46 , , , cord-329245-6tj2k1yn 70 47 lane lane NN cord-329245-6tj2k1yn 70 48 4 4 CD cord-329245-6tj2k1yn 70 49 ) ) -RRB- cord-329245-6tj2k1yn 70 50 and and CC cord-329245-6tj2k1yn 70 51 that that IN cord-329245-6tj2k1yn 70 52 E e NN cord-329245-6tj2k1yn 70 53 protein protein NN cord-329245-6tj2k1yn 70 54 was be VBD cord-329245-6tj2k1yn 70 55 present present JJ cord-329245-6tj2k1yn 70 56 in in IN cord-329245-6tj2k1yn 70 57 the the DT cord-329245-6tj2k1yn 70 58 anti anti JJ cord-329245-6tj2k1yn 70 59 - - JJ cord-329245-6tj2k1yn 70 60 M m JJ cord-329245-6tj2k1yn 70 61 immunoprecipitate immunoprecipitate NN cord-329245-6tj2k1yn 70 62 ( ( -LRB- cord-329245-6tj2k1yn 70 63 Fig Fig NNP cord-329245-6tj2k1yn 70 64 . . NNP cord-329245-6tj2k1yn 70 65 2 2 CD cord-329245-6tj2k1yn 70 66 , , , cord-329245-6tj2k1yn 70 67 lane lane NN cord-329245-6tj2k1yn 70 68 8) 8) NNP cord-329245-6tj2k1yn 70 69 , , , cord-329245-6tj2k1yn 70 70 suggesting suggest VBG cord-329245-6tj2k1yn 70 71 that that IN cord-329245-6tj2k1yn 70 72 the the DT cord-329245-6tj2k1yn 70 73 two two CD cord-329245-6tj2k1yn 70 74 proteins protein NNS cord-329245-6tj2k1yn 70 75 directly directly RB cord-329245-6tj2k1yn 70 76 interact interact VBP cord-329245-6tj2k1yn 70 77 in in IN cord-329245-6tj2k1yn 70 78 transfected transfected JJ cord-329245-6tj2k1yn 70 79 cells cell NNS cord-329245-6tj2k1yn 70 80 . . . cord-329245-6tj2k1yn 71 1 Detection detection NN cord-329245-6tj2k1yn 71 2 of of IN cord-329245-6tj2k1yn 71 3 the the DT cord-329245-6tj2k1yn 71 4 interaction interaction NN cord-329245-6tj2k1yn 71 5 required require VBN cord-329245-6tj2k1yn 71 6 crosslinking crosslinking NN cord-329245-6tj2k1yn 71 7 ( ( -LRB- cord-329245-6tj2k1yn 71 8 compare compare VB cord-329245-6tj2k1yn 71 9 lanes lane NNS cord-329245-6tj2k1yn 71 10 3 3 CD cord-329245-6tj2k1yn 71 11 and and CC cord-329245-6tj2k1yn 71 12 7 7 CD cord-329245-6tj2k1yn 71 13 to to TO cord-329245-6tj2k1yn 71 14 lanes lane NNS cord-329245-6tj2k1yn 71 15 4 4 CD cord-329245-6tj2k1yn 71 16 and and CC cord-329245-6tj2k1yn 71 17 8) 8) NN cord-329245-6tj2k1yn 71 18 and and CC cord-329245-6tj2k1yn 71 19 antisera antisera NN cord-329245-6tj2k1yn 71 20 specific specific JJ cord-329245-6tj2k1yn 71 21 for for IN cord-329245-6tj2k1yn 71 22 E E NNP cord-329245-6tj2k1yn 71 23 or or CC cord-329245-6tj2k1yn 71 24 M. m. NN cord-329245-6tj2k1yn 71 25 Even even RB cord-329245-6tj2k1yn 71 26 though though RB cord-329245-6tj2k1yn 71 27 less less JJR cord-329245-6tj2k1yn 71 28 E e NN cord-329245-6tj2k1yn 71 29 was be VBD cord-329245-6tj2k1yn 71 30 expressed express VBN cord-329245-6tj2k1yn 71 31 in in IN cord-329245-6tj2k1yn 71 32 transfected transfecte VBN cord-329245-6tj2k1yn 71 33 cells cell NNS cord-329245-6tj2k1yn 71 34 ( ( -LRB- cord-329245-6tj2k1yn 71 35 Fig fig NN cord-329245-6tj2k1yn 71 36 . . NNP cord-329245-6tj2k1yn 71 37 2 2 CD cord-329245-6tj2k1yn 71 38 ) ) -RRB- cord-329245-6tj2k1yn 71 39 compared compare VBN cord-329245-6tj2k1yn 71 40 to to IN cord-329245-6tj2k1yn 71 41 IBV IBV NNP cord-329245-6tj2k1yn 71 42 - - HYPH cord-329245-6tj2k1yn 71 43 infected infect VBN cord-329245-6tj2k1yn 71 44 cells cell NNS cord-329245-6tj2k1yn 71 45 ( ( -LRB- cord-329245-6tj2k1yn 71 46 Fig fig NN cord-329245-6tj2k1yn 71 47 . . . cord-329245-6tj2k1yn 71 48 1B 1B NNP cord-329245-6tj2k1yn 71 49 ) ) -RRB- cord-329245-6tj2k1yn 71 50 , , , cord-329245-6tj2k1yn 71 51 the the DT cord-329245-6tj2k1yn 71 52 ratio ratio NN cord-329245-6tj2k1yn 71 53 of of IN cord-329245-6tj2k1yn 71 54 E e NN cord-329245-6tj2k1yn 71 55 : : : cord-329245-6tj2k1yn 71 56 M m NN cord-329245-6tj2k1yn 71 57 crosslinks crosslink NNS cord-329245-6tj2k1yn 71 58 ( ( -LRB- cord-329245-6tj2k1yn 71 59 as as IN cord-329245-6tj2k1yn 71 60 seen see VBN cord-329245-6tj2k1yn 71 61 in in IN cord-329245-6tj2k1yn 71 62 the the DT cord-329245-6tj2k1yn 71 63 relative relative JJ cord-329245-6tj2k1yn 71 64 amounts amount NNS cord-329245-6tj2k1yn 71 65 of of IN cord-329245-6tj2k1yn 71 66 E e NN cord-329245-6tj2k1yn 71 67 and and CC cord-329245-6tj2k1yn 71 68 M M NNP cord-329245-6tj2k1yn 71 69 in in IN cord-329245-6tj2k1yn 71 70 the the DT cord-329245-6tj2k1yn 71 71 anti anti JJ cord-329245-6tj2k1yn 71 72 - - JJ cord-329245-6tj2k1yn 71 73 M m JJ cord-329245-6tj2k1yn 71 74 and and CC cord-329245-6tj2k1yn 71 75 anti anti JJ cord-329245-6tj2k1yn 71 76 - - JJ cord-329245-6tj2k1yn 71 77 E e JJ cord-329245-6tj2k1yn 71 78 immunoprecipitations immunoprecipitation NNS cord-329245-6tj2k1yn 71 79 , , , cord-329245-6tj2k1yn 71 80 respectively respectively RB cord-329245-6tj2k1yn 71 81 ) ) -RRB- cord-329245-6tj2k1yn 71 82 was be VBD cord-329245-6tj2k1yn 71 83 similar similar JJ cord-329245-6tj2k1yn 71 84 , , , cord-329245-6tj2k1yn 71 85 suggesting suggest VBG cord-329245-6tj2k1yn 71 86 that that IN cord-329245-6tj2k1yn 71 87 there there EX cord-329245-6tj2k1yn 71 88 is be VBZ cord-329245-6tj2k1yn 71 89 a a DT cord-329245-6tj2k1yn 71 90 precise precise JJ cord-329245-6tj2k1yn 71 91 stoichiometry stoichiometry NN cord-329245-6tj2k1yn 71 92 of of IN cord-329245-6tj2k1yn 71 93 interaction interaction NN cord-329245-6tj2k1yn 71 94 . . . cord-329245-6tj2k1yn 72 1 To to TO cord-329245-6tj2k1yn 72 2 investigate investigate VB cord-329245-6tj2k1yn 72 3 which which WDT cord-329245-6tj2k1yn 72 4 domains domain NNS cord-329245-6tj2k1yn 72 5 of of IN cord-329245-6tj2k1yn 72 6 the the DT cord-329245-6tj2k1yn 72 7 E E NNP cord-329245-6tj2k1yn 72 8 and and CC cord-329245-6tj2k1yn 72 9 M M NNP cord-329245-6tj2k1yn 72 10 proteins protein NNS cord-329245-6tj2k1yn 72 11 interact interact VBP cord-329245-6tj2k1yn 72 12 , , , cord-329245-6tj2k1yn 72 13 we -PRON- PRP cord-329245-6tj2k1yn 72 14 made make VBD cord-329245-6tj2k1yn 72 15 use use NN cord-329245-6tj2k1yn 72 16 of of IN cord-329245-6tj2k1yn 72 17 a a DT cord-329245-6tj2k1yn 72 18 panel panel NN cord-329245-6tj2k1yn 72 19 of of IN cord-329245-6tj2k1yn 72 20 mutant mutant JJ cord-329245-6tj2k1yn 72 21 E E NNP cord-329245-6tj2k1yn 72 22 and and CC cord-329245-6tj2k1yn 72 23 M M NNP cord-329245-6tj2k1yn 72 24 proteins protein NNS cord-329245-6tj2k1yn 72 25 that that WDT cord-329245-6tj2k1yn 72 26 were be VBD cord-329245-6tj2k1yn 72 27 generated generate VBN cord-329245-6tj2k1yn 72 28 to to TO cord-329245-6tj2k1yn 72 29 study study VB cord-329245-6tj2k1yn 72 30 the the DT cord-329245-6tj2k1yn 72 31 Golgi Golgi NNP cord-329245-6tj2k1yn 72 32 targeting targeting NN cord-329245-6tj2k1yn 72 33 signals signal NNS cord-329245-6tj2k1yn 72 34 present present JJ cord-329245-6tj2k1yn 72 35 in in IN cord-329245-6tj2k1yn 72 36 the the DT cord-329245-6tj2k1yn 72 37 two two CD cord-329245-6tj2k1yn 72 38 proteins protein NNS cord-329245-6tj2k1yn 72 39 ( ( -LRB- cord-329245-6tj2k1yn 72 40 Corse Corse NNP cord-329245-6tj2k1yn 72 41 and and CC cord-329245-6tj2k1yn 72 42 Machamer Machamer NNP cord-329245-6tj2k1yn 72 43 , , , cord-329245-6tj2k1yn 72 44 2002 2002 CD cord-329245-6tj2k1yn 72 45 ; ; : cord-329245-6tj2k1yn 72 46 Machamer Machamer NNP cord-329245-6tj2k1yn 72 47 and and CC cord-329245-6tj2k1yn 72 48 Rose Rose NNP cord-329245-6tj2k1yn 72 49 , , , cord-329245-6tj2k1yn 72 50 1987 1987 CD cord-329245-6tj2k1yn 72 51 ) ) -RRB- cord-329245-6tj2k1yn 72 52 . . . cord-329245-6tj2k1yn 73 1 Although although IN cord-329245-6tj2k1yn 73 2 many many JJ cord-329245-6tj2k1yn 73 3 mutant mutant JJ cord-329245-6tj2k1yn 73 4 E e NN cord-329245-6tj2k1yn 73 5 and and CC cord-329245-6tj2k1yn 73 6 M M NNP cord-329245-6tj2k1yn 73 7 proteins protein NNS cord-329245-6tj2k1yn 73 8 have have VBP cord-329245-6tj2k1yn 73 9 been be VBN cord-329245-6tj2k1yn 73 10 generated generate VBN cord-329245-6tj2k1yn 73 11 , , , cord-329245-6tj2k1yn 73 12 in in IN cord-329245-6tj2k1yn 73 13 this this DT cord-329245-6tj2k1yn 73 14 study study NN cord-329245-6tj2k1yn 73 15 , , , cord-329245-6tj2k1yn 73 16 we -PRON- PRP cord-329245-6tj2k1yn 73 17 used use VBD cord-329245-6tj2k1yn 73 18 only only RB cord-329245-6tj2k1yn 73 19 those those DT cord-329245-6tj2k1yn 73 20 that that WDT cord-329245-6tj2k1yn 73 21 are be VBP cord-329245-6tj2k1yn 73 22 properly properly RB cord-329245-6tj2k1yn 73 23 targeted target VBN cord-329245-6tj2k1yn 73 24 to to IN cord-329245-6tj2k1yn 73 25 the the DT cord-329245-6tj2k1yn 73 26 Golgi Golgi NNP cord-329245-6tj2k1yn 73 27 region region NN cord-329245-6tj2k1yn 73 28 . . . cord-329245-6tj2k1yn 74 1 Since since IN cord-329245-6tj2k1yn 74 2 these these DT cord-329245-6tj2k1yn 74 3 mutant mutant JJ cord-329245-6tj2k1yn 74 4 proteins protein NNS cord-329245-6tj2k1yn 74 5 had have VBD cord-329245-6tj2k1yn 74 6 previously previously RB cord-329245-6tj2k1yn 74 7 been be VBN cord-329245-6tj2k1yn 74 8 localized localize VBN cord-329245-6tj2k1yn 74 9 in in IN cord-329245-6tj2k1yn 74 10 BHK-21 BHK-21 NNP cord-329245-6tj2k1yn 74 11 or or CC cord-329245-6tj2k1yn 74 12 COS-7 COS-7 NNP cord-329245-6tj2k1yn 74 13 cells cell NNS cord-329245-6tj2k1yn 74 14 , , , cord-329245-6tj2k1yn 74 15 we -PRON- PRP cord-329245-6tj2k1yn 74 16 first first RB cord-329245-6tj2k1yn 74 17 confirmed confirm VBD cord-329245-6tj2k1yn 74 18 that that IN cord-329245-6tj2k1yn 74 19 they -PRON- PRP cord-329245-6tj2k1yn 74 20 were be VBD cord-329245-6tj2k1yn 74 21 properly properly RB cord-329245-6tj2k1yn 74 22 targeted target VBN cord-329245-6tj2k1yn 74 23 in in IN cord-329245-6tj2k1yn 74 24 OST-7 OST-7 NNP cord-329245-6tj2k1yn 74 25 cells cell NNS cord-329245-6tj2k1yn 74 26 . . . cord-329245-6tj2k1yn 75 1 Cells cell NNS cord-329245-6tj2k1yn 75 2 coexpressing coexpresse VBG cord-329245-6tj2k1yn 75 3 wildtype wildtype NN cord-329245-6tj2k1yn 75 4 M M NNP cord-329245-6tj2k1yn 75 5 protein protein NN cord-329245-6tj2k1yn 75 6 and and CC cord-329245-6tj2k1yn 75 7 E e NN cord-329245-6tj2k1yn 75 8 mutants mutant NNS cord-329245-6tj2k1yn 75 9 were be VBD cord-329245-6tj2k1yn 75 10 analyzed analyze VBN cord-329245-6tj2k1yn 75 11 by by IN cord-329245-6tj2k1yn 75 12 indirect indirect JJ cord-329245-6tj2k1yn 75 13 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 75 14 microscopy microscopy NN cord-329245-6tj2k1yn 75 15 ( ( -LRB- cord-329245-6tj2k1yn 75 16 Fig Fig NNP cord-329245-6tj2k1yn 75 17 . . . cord-329245-6tj2k1yn 75 18 3A 3A NNP cord-329245-6tj2k1yn 75 19 ) ) -RRB- cord-329245-6tj2k1yn 75 20 . . . cord-329245-6tj2k1yn 76 1 EG3 EG3 NNP cord-329245-6tj2k1yn 76 2 ( ( -LRB- cord-329245-6tj2k1yn 76 3 panels panel NNS cord-329245-6tj2k1yn 76 4 d d XX cord-329245-6tj2k1yn 76 5 - - HYPH cord-329245-6tj2k1yn 76 6 f f NNP cord-329245-6tj2k1yn 76 7 ) ) -RRB- cord-329245-6tj2k1yn 76 8 contains contain VBZ cord-329245-6tj2k1yn 76 9 the the DT cord-329245-6tj2k1yn 76 10 transmembrane transmembrane NN cord-329245-6tj2k1yn 76 11 domain domain NN cord-329245-6tj2k1yn 76 12 from from IN cord-329245-6tj2k1yn 76 13 the the DT cord-329245-6tj2k1yn 76 14 plasma plasma NN cord-329245-6tj2k1yn 76 15 membrane membrane NN cord-329245-6tj2k1yn 76 16 protein protein NN cord-329245-6tj2k1yn 76 17 vesicular vesicular JJ cord-329245-6tj2k1yn 76 18 stomatitis stomatitis NN cord-329245-6tj2k1yn 76 19 virus virus NN cord-329245-6tj2k1yn 76 20 ( ( -LRB- cord-329245-6tj2k1yn 76 21 VSV VSV NNP cord-329245-6tj2k1yn 76 22 ) ) -RRB- cord-329245-6tj2k1yn 76 23 G G NNP cord-329245-6tj2k1yn 76 24 ; ; : cord-329245-6tj2k1yn 76 25 CTE CTE NNP cord-329245-6tj2k1yn 76 26 ( ( -LRB- cord-329245-6tj2k1yn 76 27 panels panel NNS cord-329245-6tj2k1yn 76 28 g g XX cord-329245-6tj2k1yn 76 29 - - : cord-329245-6tj2k1yn 76 30 i i NNP cord-329245-6tj2k1yn 76 31 ) ) -RRB- cord-329245-6tj2k1yn 76 32 is be VBZ cord-329245-6tj2k1yn 76 33 an an DT cord-329245-6tj2k1yn 76 34 N n CD cord-329245-6tj2k1yn 76 35 - - HYPH cord-329245-6tj2k1yn 76 36 terminal terminal JJ cord-329245-6tj2k1yn 76 37 truncation truncation NN cord-329245-6tj2k1yn 76 38 of of IN cord-329245-6tj2k1yn 76 39 IBV IBV NNP cord-329245-6tj2k1yn 76 40 E E NNP cord-329245-6tj2k1yn 76 41 consisting consist VBG cord-329245-6tj2k1yn 76 42 only only RB cord-329245-6tj2k1yn 76 43 of of IN cord-329245-6tj2k1yn 76 44 its -PRON- PRP$ cord-329245-6tj2k1yn 76 45 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 76 46 tail tail NN cord-329245-6tj2k1yn 76 47 , , , cord-329245-6tj2k1yn 76 48 and and CC cord-329245-6tj2k1yn 76 49 GEt get VBP cord-329245-6tj2k1yn 76 50 ( ( -LRB- cord-329245-6tj2k1yn 76 51 panels panel NNS cord-329245-6tj2k1yn 76 52 j j NNP cord-329245-6tj2k1yn 76 53 - - HYPH cord-329245-6tj2k1yn 76 54 l l NNP cord-329245-6tj2k1yn 76 55 ) ) -RRB- cord-329245-6tj2k1yn 76 56 is be VBZ cord-329245-6tj2k1yn 76 57 a a DT cord-329245-6tj2k1yn 76 58 chimeric chimeric JJ cord-329245-6tj2k1yn 76 59 protein protein NN cord-329245-6tj2k1yn 76 60 containing contain VBG cord-329245-6tj2k1yn 76 61 the the DT cord-329245-6tj2k1yn 76 62 lumenal lumenal NNP cord-329245-6tj2k1yn 76 63 and and CC cord-329245-6tj2k1yn 76 64 transmembrane transmembrane JJ cord-329245-6tj2k1yn 76 65 domains domain NNS cord-329245-6tj2k1yn 76 66 of of IN cord-329245-6tj2k1yn 76 67 VSV VSV NNP cord-329245-6tj2k1yn 76 68 G G NNP cord-329245-6tj2k1yn 76 69 and and CC cord-329245-6tj2k1yn 76 70 the the DT cord-329245-6tj2k1yn 76 71 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 76 72 tail tail NN cord-329245-6tj2k1yn 76 73 of of IN cord-329245-6tj2k1yn 76 74 IBV IBV NNP cord-329245-6tj2k1yn 76 75 E. E. NNP cord-329245-6tj2k1yn 77 1 In in IN cord-329245-6tj2k1yn 77 2 all all DT cord-329245-6tj2k1yn 77 3 cases case NNS cord-329245-6tj2k1yn 77 4 , , , cord-329245-6tj2k1yn 77 5 the the DT cord-329245-6tj2k1yn 77 6 E e NN cord-329245-6tj2k1yn 77 7 mutant mutant NN cord-329245-6tj2k1yn 77 8 proteins protein NNS cord-329245-6tj2k1yn 77 9 were be VBD cord-329245-6tj2k1yn 77 10 correctly correctly RB cord-329245-6tj2k1yn 77 11 localized localize VBN cord-329245-6tj2k1yn 77 12 to to IN cord-329245-6tj2k1yn 77 13 the the DT cord-329245-6tj2k1yn 77 14 Golgi Golgi NNP cord-329245-6tj2k1yn 77 15 region region NN cord-329245-6tj2k1yn 77 16 of of IN cord-329245-6tj2k1yn 77 17 OST7 OST7 NNP cord-329245-6tj2k1yn 77 18 - - HYPH cord-329245-6tj2k1yn 77 19 1 1 CD cord-329245-6tj2k1yn 77 20 cells cell NNS cord-329245-6tj2k1yn 77 21 ( ( -LRB- cord-329245-6tj2k1yn 77 22 including include VBG cord-329245-6tj2k1yn 77 23 the the DT cord-329245-6tj2k1yn 77 24 partial partial JJ cord-329245-6tj2k1yn 77 25 transmembrane transmembrane NN cord-329245-6tj2k1yn 77 26 replacement replacement NN cord-329245-6tj2k1yn 77 27 mutants mutant NNS cord-329245-6tj2k1yn 77 28 EG1 EG1 NNP cord-329245-6tj2k1yn 77 29 and and CC cord-329245-6tj2k1yn 77 30 EG2 EG2 NNP cord-329245-6tj2k1yn 77 31 , , , cord-329245-6tj2k1yn 77 32 not not RB cord-329245-6tj2k1yn 77 33 shown show VBN cord-329245-6tj2k1yn 77 34 ) ) -RRB- cord-329245-6tj2k1yn 77 35 , , , cord-329245-6tj2k1yn 77 36 consistent consistent JJ cord-329245-6tj2k1yn 77 37 with with IN cord-329245-6tj2k1yn 77 38 our -PRON- PRP$ cord-329245-6tj2k1yn 77 39 previous previous JJ cord-329245-6tj2k1yn 77 40 studies study NNS cord-329245-6tj2k1yn 77 41 in in IN cord-329245-6tj2k1yn 77 42 BHK-21 BHK-21 NNP cord-329245-6tj2k1yn 77 43 cells cell NNS cord-329245-6tj2k1yn 77 44 ( ( -LRB- cord-329245-6tj2k1yn 77 45 Corse Corse NNP cord-329245-6tj2k1yn 77 46 and and CC cord-329245-6tj2k1yn 77 47 Machamer Machamer NNP cord-329245-6tj2k1yn 77 48 , , , cord-329245-6tj2k1yn 77 49 2002 2002 CD cord-329245-6tj2k1yn 77 50 ) ) -RRB- cord-329245-6tj2k1yn 77 51 . . . cord-329245-6tj2k1yn 78 1 Fig Fig NNP cord-329245-6tj2k1yn 78 2 . . . cord-329245-6tj2k1yn 79 1 3B 3B NNP cord-329245-6tj2k1yn 79 2 shows show VBZ cord-329245-6tj2k1yn 79 3 cells cell NNS cord-329245-6tj2k1yn 79 4 that that WDT cord-329245-6tj2k1yn 79 5 are be VBP cord-329245-6tj2k1yn 79 6 coexpressing coexpresse VBG cord-329245-6tj2k1yn 79 7 wild wild JJ cord-329245-6tj2k1yn 79 8 - - HYPH cord-329245-6tj2k1yn 79 9 type type NN cord-329245-6tj2k1yn 79 10 E e NN cord-329245-6tj2k1yn 79 11 protein protein NN cord-329245-6tj2k1yn 79 12 and and CC cord-329245-6tj2k1yn 79 13 the the DT cord-329245-6tj2k1yn 79 14 M M NNP cord-329245-6tj2k1yn 79 15 protein protein NN cord-329245-6tj2k1yn 79 16 deletion deletion NN cord-329245-6tj2k1yn 79 17 mutants mutant NNS cord-329245-6tj2k1yn 79 18 Mct mct VBP cord-329245-6tj2k1yn 79 19 ⌬ ⌬ NNS cord-329245-6tj2k1yn 79 20 1 1 CD cord-329245-6tj2k1yn 80 1 ( ( -LRB- cord-329245-6tj2k1yn 80 2 panels panel VBZ cord-329245-6tj2k1yn 80 3 a a DT cord-329245-6tj2k1yn 80 4 - - HYPH cord-329245-6tj2k1yn 80 5 c c NN cord-329245-6tj2k1yn 80 6 ) ) -RRB- cord-329245-6tj2k1yn 80 7 , , , cord-329245-6tj2k1yn 80 8 which which WDT cord-329245-6tj2k1yn 80 9 is be VBZ cord-329245-6tj2k1yn 80 10 missing miss VBG cord-329245-6tj2k1yn 80 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 80 12 tail tail NN cord-329245-6tj2k1yn 80 13 amino amino NN cord-329245-6tj2k1yn 80 14 acid acid NN cord-329245-6tj2k1yn 80 15 residues residue NNS cord-329245-6tj2k1yn 80 16 103 103 CD cord-329245-6tj2k1yn 80 17 - - HYPH cord-329245-6tj2k1yn 80 18 203 203 CD cord-329245-6tj2k1yn 80 19 , , , cord-329245-6tj2k1yn 80 20 Mct Mct NNP cord-329245-6tj2k1yn 80 21 ⌬ ⌬ NNS cord-329245-6tj2k1yn 80 22 2 2 CD cord-329245-6tj2k1yn 81 1 ( ( -LRB- cord-329245-6tj2k1yn 81 2 panels panel NNS cord-329245-6tj2k1yn 81 3 d d XX cord-329245-6tj2k1yn 81 4 - - HYPH cord-329245-6tj2k1yn 81 5 f f NNP cord-329245-6tj2k1yn 81 6 ) ) -RRB- cord-329245-6tj2k1yn 81 7 , , , cord-329245-6tj2k1yn 81 8 which which WDT cord-329245-6tj2k1yn 81 9 is be VBZ cord-329245-6tj2k1yn 81 10 missing miss VBG cord-329245-6tj2k1yn 81 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 81 12 tail tail NN cord-329245-6tj2k1yn 81 13 residues residue NNS cord-329245-6tj2k1yn 81 14 119 119 CD cord-329245-6tj2k1yn 81 15 -203 -203 NN cord-329245-6tj2k1yn 81 16 , , , cord-329245-6tj2k1yn 81 17 Mct Mct NNP cord-329245-6tj2k1yn 81 18 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 81 19 3 3 CD cord-329245-6tj2k1yn 82 1 ( ( -LRB- cord-329245-6tj2k1yn 82 2 panels panel NNS cord-329245-6tj2k1yn 82 3 g g NNP cord-329245-6tj2k1yn 82 4 - - : cord-329245-6tj2k1yn 82 5 i i NNP cord-329245-6tj2k1yn 82 6 ) ) -RRB- cord-329245-6tj2k1yn 82 7 , , , cord-329245-6tj2k1yn 82 8 which which WDT cord-329245-6tj2k1yn 82 9 is be VBZ cord-329245-6tj2k1yn 82 10 missing miss VBG cord-329245-6tj2k1yn 82 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 82 12 tail tail NN cord-329245-6tj2k1yn 82 13 residues residue NNS cord-329245-6tj2k1yn 82 14 103 103 CD cord-329245-6tj2k1yn 82 15 - - SYM cord-329245-6tj2k1yn 82 16 118 118 CD cord-329245-6tj2k1yn 82 17 , , , cord-329245-6tj2k1yn 82 18 or or CC cord-329245-6tj2k1yn 82 19 M M NNP cord-329245-6tj2k1yn 82 20 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 82 21 m2,3 m2,3 NNP cord-329245-6tj2k1yn 82 22 ( ( -LRB- cord-329245-6tj2k1yn 82 23 panels panel NNS cord-329245-6tj2k1yn 82 24 j j NNP cord-329245-6tj2k1yn 82 25 - - HYPH cord-329245-6tj2k1yn 82 26 l l NNP cord-329245-6tj2k1yn 82 27 ) ) -RRB- cord-329245-6tj2k1yn 82 28 , , , cord-329245-6tj2k1yn 82 29 which which WDT cord-329245-6tj2k1yn 82 30 is be VBZ cord-329245-6tj2k1yn 82 31 missing miss VBG cord-329245-6tj2k1yn 82 32 the the DT cord-329245-6tj2k1yn 82 33 second second JJ cord-329245-6tj2k1yn 82 34 and and CC cord-329245-6tj2k1yn 82 35 third third JJ cord-329245-6tj2k1yn 82 36 transmembrane transmembrane NN cord-329245-6tj2k1yn 82 37 domains domain NNS cord-329245-6tj2k1yn 82 38 ( ( -LRB- cord-329245-6tj2k1yn 82 39 amino amino NN cord-329245-6tj2k1yn 82 40 acid acid NN cord-329245-6tj2k1yn 82 41 residues residue NNS cord-329245-6tj2k1yn 82 42 43 43 CD cord-329245-6tj2k1yn 82 43 - - SYM cord-329245-6tj2k1yn 82 44 101 101 CD cord-329245-6tj2k1yn 82 45 ) ) -RRB- cord-329245-6tj2k1yn 82 46 . . . cord-329245-6tj2k1yn 83 1 The the DT cord-329245-6tj2k1yn 83 2 M M NNP cord-329245-6tj2k1yn 83 3 mutants mutant NNS cord-329245-6tj2k1yn 83 4 , , , cord-329245-6tj2k1yn 83 5 especially especially RB cord-329245-6tj2k1yn 83 6 Mct Mct NNP cord-329245-6tj2k1yn 83 7 ⌬ ⌬ NNS cord-329245-6tj2k1yn 83 8 3 3 CD cord-329245-6tj2k1yn 84 1 ( ( -LRB- cord-329245-6tj2k1yn 84 2 Fig Fig NNP cord-329245-6tj2k1yn 84 3 . . . cord-329245-6tj2k1yn 84 4 3B 3B NNP cord-329245-6tj2k1yn 84 5 , , , cord-329245-6tj2k1yn 84 6 panel panel NN cord-329245-6tj2k1yn 84 7 h h NNP cord-329245-6tj2k1yn 84 8 ) ) -RRB- cord-329245-6tj2k1yn 84 9 and and CC cord-329245-6tj2k1yn 84 10 M M NNP cord-329245-6tj2k1yn 84 11 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 84 12 m2,3 m2,3 NNP cord-329245-6tj2k1yn 84 13 ( ( -LRB- cord-329245-6tj2k1yn 84 14 Fig Fig NNP cord-329245-6tj2k1yn 84 15 . . . cord-329245-6tj2k1yn 84 16 3B 3B NNP cord-329245-6tj2k1yn 84 17 , , , cord-329245-6tj2k1yn 84 18 panel panel NN cord-329245-6tj2k1yn 84 19 k k NNP cord-329245-6tj2k1yn 84 20 ) ) -RRB- cord-329245-6tj2k1yn 84 21 , , , cord-329245-6tj2k1yn 84 22 were be VBD cord-329245-6tj2k1yn 84 23 incompletely incompletely RB cord-329245-6tj2k1yn 84 24 localized localize VBN cord-329245-6tj2k1yn 84 25 to to IN cord-329245-6tj2k1yn 84 26 the the DT cord-329245-6tj2k1yn 84 27 Golgi Golgi NNP cord-329245-6tj2k1yn 84 28 region region NN cord-329245-6tj2k1yn 84 29 , , , cord-329245-6tj2k1yn 84 30 since since IN cord-329245-6tj2k1yn 84 31 some some DT cord-329245-6tj2k1yn 84 32 ER er NN cord-329245-6tj2k1yn 84 33 staining staining NN cord-329245-6tj2k1yn 84 34 was be VBD cord-329245-6tj2k1yn 84 35 observed observe VBN cord-329245-6tj2k1yn 84 36 . . . cord-329245-6tj2k1yn 85 1 However however RB cord-329245-6tj2k1yn 85 2 , , , cord-329245-6tj2k1yn 85 3 they -PRON- PRP cord-329245-6tj2k1yn 85 4 all all DT cord-329245-6tj2k1yn 85 5 reached reach VBD cord-329245-6tj2k1yn 85 6 the the DT cord-329245-6tj2k1yn 85 7 Golgi Golgi NNP cord-329245-6tj2k1yn 85 8 to to IN cord-329245-6tj2k1yn 85 9 some some DT cord-329245-6tj2k1yn 85 10 extent extent NN cord-329245-6tj2k1yn 85 11 , , , cord-329245-6tj2k1yn 85 12 as as IN cord-329245-6tj2k1yn 85 13 indicated indicate VBN cord-329245-6tj2k1yn 85 14 by by IN cord-329245-6tj2k1yn 85 15 the the DT cord-329245-6tj2k1yn 85 16 juxtanuclear juxtanuclear NNP cord-329245-6tj2k1yn 85 17 spot spot NN cord-329245-6tj2k1yn 85 18 . . . cord-329245-6tj2k1yn 86 1 We -PRON- PRP cord-329245-6tj2k1yn 86 2 asked ask VBD cord-329245-6tj2k1yn 86 3 whether whether IN cord-329245-6tj2k1yn 86 4 the the DT cord-329245-6tj2k1yn 86 5 E e NN cord-329245-6tj2k1yn 86 6 transmembrane transmembrane NN cord-329245-6tj2k1yn 86 7 replacement replacement NN cord-329245-6tj2k1yn 86 8 mutants mutant NNS cord-329245-6tj2k1yn 86 9 EG1 EG1 NNP cord-329245-6tj2k1yn 86 10 , , , cord-329245-6tj2k1yn 86 11 EG2 EG2 NNP cord-329245-6tj2k1yn 86 12 , , , cord-329245-6tj2k1yn 86 13 and and CC cord-329245-6tj2k1yn 86 14 EG3 eg3 NN cord-329245-6tj2k1yn 86 15 ( ( -LRB- cord-329245-6tj2k1yn 86 16 in in IN cord-329245-6tj2k1yn 86 17 which which WDT cord-329245-6tj2k1yn 86 18 the the DT cord-329245-6tj2k1yn 86 19 first first JJ cord-329245-6tj2k1yn 86 20 third third NN cord-329245-6tj2k1yn 86 21 , , , cord-329245-6tj2k1yn 86 22 the the DT cord-329245-6tj2k1yn 86 23 first first JJ cord-329245-6tj2k1yn 86 24 two two CD cord-329245-6tj2k1yn 86 25 - - HYPH cord-329245-6tj2k1yn 86 26 thirds third NNS cord-329245-6tj2k1yn 86 27 , , , cord-329245-6tj2k1yn 86 28 or or CC cord-329245-6tj2k1yn 86 29 the the DT cord-329245-6tj2k1yn 86 30 entire entire JJ cord-329245-6tj2k1yn 86 31 E e NN cord-329245-6tj2k1yn 86 32 transmembrane transmembrane NN cord-329245-6tj2k1yn 86 33 domain domain NN cord-329245-6tj2k1yn 86 34 , , , cord-329245-6tj2k1yn 86 35 respectively respectively RB cord-329245-6tj2k1yn 86 36 , , , cord-329245-6tj2k1yn 86 37 were be VBD cord-329245-6tj2k1yn 86 38 replaced replace VBN cord-329245-6tj2k1yn 86 39 with with IN cord-329245-6tj2k1yn 86 40 the the DT cord-329245-6tj2k1yn 86 41 corresponding correspond VBG cord-329245-6tj2k1yn 86 42 regions region NNS cord-329245-6tj2k1yn 86 43 of of IN cord-329245-6tj2k1yn 86 44 the the DT cord-329245-6tj2k1yn 86 45 VSV VSV NNP cord-329245-6tj2k1yn 86 46 G G NNP cord-329245-6tj2k1yn 86 47 transmembrane transmembrane NN cord-329245-6tj2k1yn 86 48 domain domain NN cord-329245-6tj2k1yn 86 49 ) ) -RRB- cord-329245-6tj2k1yn 87 1 ( ( -LRB- cord-329245-6tj2k1yn 87 2 Corse Corse NNP cord-329245-6tj2k1yn 87 3 and and CC cord-329245-6tj2k1yn 87 4 Machamer Machamer NNP cord-329245-6tj2k1yn 87 5 , , , cord-329245-6tj2k1yn 87 6 2002 2002 CD cord-329245-6tj2k1yn 87 7 ) ) -RRB- cord-329245-6tj2k1yn 87 8 could could MD cord-329245-6tj2k1yn 87 9 be be VB cord-329245-6tj2k1yn 87 10 crosslinked crosslinke VBN cord-329245-6tj2k1yn 87 11 to to IN cord-329245-6tj2k1yn 87 12 wild wild JJ cord-329245-6tj2k1yn 87 13 - - HYPH cord-329245-6tj2k1yn 87 14 type type NN cord-329245-6tj2k1yn 87 15 M M NNP cord-329245-6tj2k1yn 87 16 protein protein NN cord-329245-6tj2k1yn 87 17 . . . cord-329245-6tj2k1yn 88 1 The the DT cord-329245-6tj2k1yn 88 2 appropriate appropriate JJ cord-329245-6tj2k1yn 88 3 proteins protein NNS cord-329245-6tj2k1yn 88 4 were be VBD cord-329245-6tj2k1yn 88 5 coexpressed coexpresse VBN cord-329245-6tj2k1yn 88 6 and and CC cord-329245-6tj2k1yn 88 7 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 88 8 with with IN cord-329245-6tj2k1yn 88 9 anti anti JJ cord-329245-6tj2k1yn 88 10 - - JJ cord-329245-6tj2k1yn 88 11 E e JJ cord-329245-6tj2k1yn 88 12 or or CC cord-329245-6tj2k1yn 88 13 anti anti JJ cord-329245-6tj2k1yn 88 14 - - JJ cord-329245-6tj2k1yn 88 15 M m JJ cord-329245-6tj2k1yn 88 16 antibodies antibody NNS cord-329245-6tj2k1yn 88 17 after after IN cord-329245-6tj2k1yn 88 18 treating treat VBG cord-329245-6tj2k1yn 88 19 the the DT cord-329245-6tj2k1yn 88 20 cells cell NNS cord-329245-6tj2k1yn 88 21 with with IN cord-329245-6tj2k1yn 88 22 DSP DSP NNP cord-329245-6tj2k1yn 88 23 as as IN cord-329245-6tj2k1yn 88 24 described describe VBN cord-329245-6tj2k1yn 88 25 above above IN cord-329245-6tj2k1yn 88 26 ( ( -LRB- cord-329245-6tj2k1yn 88 27 Fig Fig NNP cord-329245-6tj2k1yn 88 28 . . . cord-329245-6tj2k1yn 88 29 4A 4a CD cord-329245-6tj2k1yn 88 30 ) ) -RRB- cord-329245-6tj2k1yn 88 31 . . . cord-329245-6tj2k1yn 89 1 All all DT cord-329245-6tj2k1yn 89 2 three three CD cord-329245-6tj2k1yn 89 3 E e NN cord-329245-6tj2k1yn 89 4 transmembrane transmembrane NN cord-329245-6tj2k1yn 89 5 replacement replacement NN cord-329245-6tj2k1yn 89 6 mutants mutant NNS cord-329245-6tj2k1yn 89 7 were be VBD cord-329245-6tj2k1yn 89 8 crosslinked crosslinke VBN cord-329245-6tj2k1yn 89 9 to to IN cord-329245-6tj2k1yn 89 10 M M NNP cord-329245-6tj2k1yn 89 11 protein protein NN cord-329245-6tj2k1yn 89 12 , , , cord-329245-6tj2k1yn 89 13 suggesting suggest VBG cord-329245-6tj2k1yn 89 14 that that IN cord-329245-6tj2k1yn 89 15 the the DT cord-329245-6tj2k1yn 89 16 E e NN cord-329245-6tj2k1yn 89 17 transmembrane transmembrane NN cord-329245-6tj2k1yn 89 18 domain domain NN cord-329245-6tj2k1yn 89 19 is be VBZ cord-329245-6tj2k1yn 89 20 not not RB cord-329245-6tj2k1yn 89 21 required require VBN cord-329245-6tj2k1yn 89 22 for for IN cord-329245-6tj2k1yn 89 23 E E NNP cord-329245-6tj2k1yn 89 24 and and CC cord-329245-6tj2k1yn 89 25 M M NNP cord-329245-6tj2k1yn 89 26 interaction interaction NN cord-329245-6tj2k1yn 89 27 . . . cord-329245-6tj2k1yn 90 1 However however RB cord-329245-6tj2k1yn 90 2 , , , cord-329245-6tj2k1yn 90 3 there there EX cord-329245-6tj2k1yn 90 4 was be VBD cord-329245-6tj2k1yn 90 5 less less JJR cord-329245-6tj2k1yn 90 6 M M NNP cord-329245-6tj2k1yn 90 7 in in IN cord-329245-6tj2k1yn 90 8 the the DT cord-329245-6tj2k1yn 90 9 anti anti JJ cord-329245-6tj2k1yn 90 10 - - JJ cord-329245-6tj2k1yn 90 11 E e JJ cord-329245-6tj2k1yn 90 12 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 90 13 of of IN cord-329245-6tj2k1yn 90 14 EG3 eg3 NN cord-329245-6tj2k1yn 91 1 ( ( -LRB- cord-329245-6tj2k1yn 91 2 Fig Fig NNP cord-329245-6tj2k1yn 91 3 . . . cord-329245-6tj2k1yn 91 4 4A 4a CD cord-329245-6tj2k1yn 91 5 , , , cord-329245-6tj2k1yn 91 6 lane lane NN cord-329245-6tj2k1yn 91 7 4 4 CD cord-329245-6tj2k1yn 91 8 ) ) -RRB- cord-329245-6tj2k1yn 91 9 , , , cord-329245-6tj2k1yn 91 10 suggesting suggest VBG cord-329245-6tj2k1yn 91 11 that that IN cord-329245-6tj2k1yn 91 12 the the DT cord-329245-6tj2k1yn 91 13 interaction interaction NN cord-329245-6tj2k1yn 91 14 of of IN cord-329245-6tj2k1yn 91 15 M M NNP cord-329245-6tj2k1yn 91 16 with with IN cord-329245-6tj2k1yn 91 17 this this DT cord-329245-6tj2k1yn 91 18 mutant mutant NN cord-329245-6tj2k1yn 91 19 could could MD cord-329245-6tj2k1yn 91 20 be be VB cord-329245-6tj2k1yn 91 21 decreased decrease VBN cord-329245-6tj2k1yn 91 22 . . . cord-329245-6tj2k1yn 92 1 Perhaps perhaps RB cord-329245-6tj2k1yn 92 2 the the DT cord-329245-6tj2k1yn 92 3 E e NN cord-329245-6tj2k1yn 92 4 and and CC cord-329245-6tj2k1yn 92 5 M M NNP cord-329245-6tj2k1yn 92 6 transmembrane transmembrane NNP cord-329245-6tj2k1yn 92 7 domains domain NNS cord-329245-6tj2k1yn 92 8 interact interact VBP cord-329245-6tj2k1yn 92 9 , , , cord-329245-6tj2k1yn 92 10 or or CC cord-329245-6tj2k1yn 92 11 the the DT cord-329245-6tj2k1yn 92 12 conformation conformation NN cord-329245-6tj2k1yn 92 13 of of IN cord-329245-6tj2k1yn 92 14 the the DT cord-329245-6tj2k1yn 92 15 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 92 16 Fig Fig NNP cord-329245-6tj2k1yn 92 17 . . . cord-329245-6tj2k1yn 93 1 2 2 LS cord-329245-6tj2k1yn 93 2 . . . cord-329245-6tj2k1yn 94 1 IBV IBV NNP cord-329245-6tj2k1yn 94 2 E E NNP cord-329245-6tj2k1yn 94 3 and and CC cord-329245-6tj2k1yn 94 4 M M NNP cord-329245-6tj2k1yn 94 5 interact interact VBP cord-329245-6tj2k1yn 94 6 in in IN cord-329245-6tj2k1yn 94 7 transfected transfecte VBN cord-329245-6tj2k1yn 94 8 OST7 OST7 NNP cord-329245-6tj2k1yn 94 9 - - HYPH cord-329245-6tj2k1yn 94 10 1 1 CD cord-329245-6tj2k1yn 94 11 cells cell NNS cord-329245-6tj2k1yn 94 12 . . . cord-329245-6tj2k1yn 95 1 OST7 OST7 NNP cord-329245-6tj2k1yn 95 2 - - HYPH cord-329245-6tj2k1yn 95 3 1 1 CD cord-329245-6tj2k1yn 95 4 cells cell NNS cord-329245-6tj2k1yn 95 5 expressing express VBG cord-329245-6tj2k1yn 95 6 E E NNP cord-329245-6tj2k1yn 95 7 and and CC cord-329245-6tj2k1yn 95 8 M M NNP cord-329245-6tj2k1yn 95 9 proteins protein NNS cord-329245-6tj2k1yn 95 10 alone alone RB cord-329245-6tj2k1yn 95 11 or or CC cord-329245-6tj2k1yn 95 12 together together RB cord-329245-6tj2k1yn 95 13 were be VBD cord-329245-6tj2k1yn 95 14 labeled label VBN cord-329245-6tj2k1yn 95 15 with with IN cord-329245-6tj2k1yn 95 16 [ [ -LRB- cord-329245-6tj2k1yn 95 17 35 35 CD cord-329245-6tj2k1yn 95 18 S]methionine S]methionine NNP cord-329245-6tj2k1yn 95 19 - - HYPH cord-329245-6tj2k1yn 95 20 cysteine cysteine NN cord-329245-6tj2k1yn 95 21 , , , cord-329245-6tj2k1yn 95 22 treated treat VBN cord-329245-6tj2k1yn 95 23 with with IN cord-329245-6tj2k1yn 95 24 DSP DSP NNP cord-329245-6tj2k1yn 95 25 as as IN cord-329245-6tj2k1yn 95 26 indicated indicate VBN cord-329245-6tj2k1yn 95 27 , , , cord-329245-6tj2k1yn 95 28 lysed lyse VBN cord-329245-6tj2k1yn 95 29 , , , cord-329245-6tj2k1yn 95 30 and and CC cord-329245-6tj2k1yn 95 31 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 95 32 with with IN cord-329245-6tj2k1yn 95 33 anti anti JJ cord-329245-6tj2k1yn 95 34 - - JJ cord-329245-6tj2k1yn 95 35 E e JJ cord-329245-6tj2k1yn 95 36 or or CC cord-329245-6tj2k1yn 95 37 anti anti JJ cord-329245-6tj2k1yn 95 38 - - JJ cord-329245-6tj2k1yn 95 39 M m JJ cord-329245-6tj2k1yn 95 40 antibodies antibody NNS cord-329245-6tj2k1yn 95 41 as as IN cord-329245-6tj2k1yn 95 42 described describe VBN cord-329245-6tj2k1yn 95 43 under under IN cord-329245-6tj2k1yn 95 44 Materials material NNS cord-329245-6tj2k1yn 95 45 and and CC cord-329245-6tj2k1yn 95 46 methods method NNS cord-329245-6tj2k1yn 95 47 . . . cord-329245-6tj2k1yn 96 1 All all DT cord-329245-6tj2k1yn 96 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 96 3 in in IN cord-329245-6tj2k1yn 96 4 this this DT cord-329245-6tj2k1yn 96 5 and and CC cord-329245-6tj2k1yn 96 6 the the DT cord-329245-6tj2k1yn 96 7 following follow VBG cord-329245-6tj2k1yn 96 8 figures figure NNS cord-329245-6tj2k1yn 96 9 were be VBD cord-329245-6tj2k1yn 96 10 deglycosylated deglycosylate VBN cord-329245-6tj2k1yn 96 11 by by IN cord-329245-6tj2k1yn 96 12 treating treat VBG cord-329245-6tj2k1yn 96 13 with with IN cord-329245-6tj2k1yn 96 14 N n NN cord-329245-6tj2k1yn 96 15 - - NN cord-329245-6tj2k1yn 96 16 glycanase glycanase NN cord-329245-6tj2k1yn 96 17 to to TO cord-329245-6tj2k1yn 96 18 collapse collapse VB cord-329245-6tj2k1yn 96 19 the the DT cord-329245-6tj2k1yn 96 20 M M NNP cord-329245-6tj2k1yn 96 21 protein protein NN cord-329245-6tj2k1yn 96 22 to to IN cord-329245-6tj2k1yn 96 23 a a DT cord-329245-6tj2k1yn 96 24 single single JJ cord-329245-6tj2k1yn 96 25 band band NN cord-329245-6tj2k1yn 96 26 . . . cord-329245-6tj2k1yn 97 1 The the DT cord-329245-6tj2k1yn 97 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 97 3 were be VBD cord-329245-6tj2k1yn 97 4 analyzed analyze VBN cord-329245-6tj2k1yn 97 5 by by IN cord-329245-6tj2k1yn 97 6 SDS-17.5 SDS-17.5 NNP cord-329245-6tj2k1yn 97 7 % % NN cord-329245-6tj2k1yn 97 8 PAGE page NN cord-329245-6tj2k1yn 97 9 in in IN cord-329245-6tj2k1yn 97 10 the the DT cord-329245-6tj2k1yn 97 11 presence presence NN cord-329245-6tj2k1yn 97 12 of of IN cord-329245-6tj2k1yn 97 13 ␤ ␤ NNP cord-329245-6tj2k1yn 97 14 ME ME NNP cord-329245-6tj2k1yn 97 15 , , , cord-329245-6tj2k1yn 97 16 and and CC cord-329245-6tj2k1yn 97 17 fluorography fluorography NNP cord-329245-6tj2k1yn 97 18 . . . cord-329245-6tj2k1yn 98 1 The the DT cord-329245-6tj2k1yn 98 2 asterisk asterisk NN cord-329245-6tj2k1yn 98 3 indicates indicate VBZ cord-329245-6tj2k1yn 98 4 a a DT cord-329245-6tj2k1yn 98 5 band band NN cord-329245-6tj2k1yn 98 6 that that WDT cord-329245-6tj2k1yn 98 7 corresponds correspond VBZ cord-329245-6tj2k1yn 98 8 to to IN cord-329245-6tj2k1yn 98 9 M M NNP cord-329245-6tj2k1yn 98 10 protein protein NN cord-329245-6tj2k1yn 98 11 dimers dimer NNS cord-329245-6tj2k1yn 98 12 . . . cord-329245-6tj2k1yn 99 1 These these DT cord-329245-6tj2k1yn 99 2 data datum NNS cord-329245-6tj2k1yn 99 3 are be VBP cord-329245-6tj2k1yn 99 4 representative representative JJ cord-329245-6tj2k1yn 99 5 of of IN cord-329245-6tj2k1yn 99 6 at at RB cord-329245-6tj2k1yn 99 7 least least RBS cord-329245-6tj2k1yn 99 8 three three CD cord-329245-6tj2k1yn 99 9 independent independent JJ cord-329245-6tj2k1yn 99 10 experiments experiment NNS cord-329245-6tj2k1yn 99 11 . . . cord-329245-6tj2k1yn 100 1 tail tail NN cord-329245-6tj2k1yn 100 2 of of IN cord-329245-6tj2k1yn 100 3 E e NN cord-329245-6tj2k1yn 100 4 is be VBZ cord-329245-6tj2k1yn 100 5 altered alter VBN cord-329245-6tj2k1yn 100 6 when when WRB cord-329245-6tj2k1yn 100 7 the the DT cord-329245-6tj2k1yn 100 8 complete complete JJ cord-329245-6tj2k1yn 100 9 transmembrane transmembrane NN cord-329245-6tj2k1yn 100 10 domain domain NN cord-329245-6tj2k1yn 100 11 is be VBZ cord-329245-6tj2k1yn 100 12 replaced replace VBN cord-329245-6tj2k1yn 100 13 ( ( -LRB- cord-329245-6tj2k1yn 100 14 see see VB cord-329245-6tj2k1yn 100 15 below below RB cord-329245-6tj2k1yn 100 16 ) ) -RRB- cord-329245-6tj2k1yn 100 17 . . . cord-329245-6tj2k1yn 101 1 To to TO cord-329245-6tj2k1yn 101 2 evaluate evaluate VB cord-329245-6tj2k1yn 101 3 the the DT cord-329245-6tj2k1yn 101 4 role role NN cord-329245-6tj2k1yn 101 5 of of IN cord-329245-6tj2k1yn 101 6 the the DT cord-329245-6tj2k1yn 101 7 E e NN cord-329245-6tj2k1yn 101 8 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 101 9 tail tail NN cord-329245-6tj2k1yn 101 10 in in IN cord-329245-6tj2k1yn 101 11 interaction interaction NN cord-329245-6tj2k1yn 101 12 with with IN cord-329245-6tj2k1yn 101 13 M M NNP cord-329245-6tj2k1yn 101 14 , , , cord-329245-6tj2k1yn 101 15 we -PRON- PRP cord-329245-6tj2k1yn 101 16 tested test VBD cord-329245-6tj2k1yn 101 17 whether whether IN cord-329245-6tj2k1yn 101 18 the the DT cord-329245-6tj2k1yn 101 19 chimeric chimeric JJ cord-329245-6tj2k1yn 101 20 protein protein NN cord-329245-6tj2k1yn 101 21 GEt GEt NNS cord-329245-6tj2k1yn 101 22 ( ( -LRB- cord-329245-6tj2k1yn 101 23 which which WDT cord-329245-6tj2k1yn 101 24 consists consist VBZ cord-329245-6tj2k1yn 101 25 of of IN cord-329245-6tj2k1yn 101 26 the the DT cord-329245-6tj2k1yn 101 27 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 101 28 tail tail NN cord-329245-6tj2k1yn 101 29 of of IN cord-329245-6tj2k1yn 101 30 IBV IBV NNP cord-329245-6tj2k1yn 101 31 E E NNP cord-329245-6tj2k1yn 101 32 and and CC cord-329245-6tj2k1yn 101 33 the the DT cord-329245-6tj2k1yn 101 34 transmembrane transmembrane NN cord-329245-6tj2k1yn 101 35 and and CC cord-329245-6tj2k1yn 101 36 lumenal lumenal JJ cord-329245-6tj2k1yn 101 37 domains domain NNS cord-329245-6tj2k1yn 101 38 of of IN cord-329245-6tj2k1yn 101 39 VSV VSV NNP cord-329245-6tj2k1yn 101 40 G G NNP cord-329245-6tj2k1yn 101 41 ; ; : cord-329245-6tj2k1yn 101 42 see see VB cord-329245-6tj2k1yn 101 43 EG3 eg3 JJ cord-329245-6tj2k1yn 101 44 and and CC cord-329245-6tj2k1yn 101 45 wild wild JJ cord-329245-6tj2k1yn 101 46 - - HYPH cord-329245-6tj2k1yn 101 47 type type NN cord-329245-6tj2k1yn 101 48 M M NNP cord-329245-6tj2k1yn 101 49 protein protein NN cord-329245-6tj2k1yn 101 50 ( ( -LRB- cord-329245-6tj2k1yn 101 51 d d NN cord-329245-6tj2k1yn 101 52 - - HYPH cord-329245-6tj2k1yn 101 53 f f NNP cord-329245-6tj2k1yn 101 54 ) ) -RRB- cord-329245-6tj2k1yn 101 55 , , , cord-329245-6tj2k1yn 101 56 CTE CTE NNP cord-329245-6tj2k1yn 101 57 and and CC cord-329245-6tj2k1yn 101 58 wild wild JJ cord-329245-6tj2k1yn 101 59 - - HYPH cord-329245-6tj2k1yn 101 60 type type NN cord-329245-6tj2k1yn 101 61 M M NNP cord-329245-6tj2k1yn 101 62 protein protein NN cord-329245-6tj2k1yn 101 63 ( ( -LRB- cord-329245-6tj2k1yn 101 64 g g NN cord-329245-6tj2k1yn 101 65 - - : cord-329245-6tj2k1yn 101 66 i i NNP cord-329245-6tj2k1yn 101 67 ) ) -RRB- cord-329245-6tj2k1yn 101 68 , , , cord-329245-6tj2k1yn 101 69 or or CC cord-329245-6tj2k1yn 101 70 GEt get VB cord-329245-6tj2k1yn 101 71 and and CC cord-329245-6tj2k1yn 101 72 wild wild JJ cord-329245-6tj2k1yn 101 73 - - HYPH cord-329245-6tj2k1yn 101 74 type type NN cord-329245-6tj2k1yn 101 75 M M NNP cord-329245-6tj2k1yn 101 76 protein protein NN cord-329245-6tj2k1yn 101 77 ( ( -LRB- cord-329245-6tj2k1yn 101 78 j j NNP cord-329245-6tj2k1yn 101 79 - - HYPH cord-329245-6tj2k1yn 101 80 l l NNP cord-329245-6tj2k1yn 101 81 ) ) -RRB- cord-329245-6tj2k1yn 101 82 were be VBD cord-329245-6tj2k1yn 101 83 fixed fix VBN cord-329245-6tj2k1yn 101 84 for for IN cord-329245-6tj2k1yn 101 85 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 101 86 and and CC cord-329245-6tj2k1yn 101 87 double double JJ cord-329245-6tj2k1yn 101 88 labeled label VBN cord-329245-6tj2k1yn 101 89 with with IN cord-329245-6tj2k1yn 101 90 rat rat NN cord-329245-6tj2k1yn 101 91 anti anti JJ cord-329245-6tj2k1yn 101 92 - - JJ cord-329245-6tj2k1yn 101 93 E e JJ cord-329245-6tj2k1yn 101 94 antibody antibody NN cord-329245-6tj2k1yn 101 95 ( ( -LRB- cord-329245-6tj2k1yn 101 96 a a DT cord-329245-6tj2k1yn 101 97 , , , cord-329245-6tj2k1yn 101 98 d d NNP cord-329245-6tj2k1yn 101 99 , , , cord-329245-6tj2k1yn 101 100 g g NNP cord-329245-6tj2k1yn 101 101 , , , cord-329245-6tj2k1yn 101 102 and and CC cord-329245-6tj2k1yn 101 103 j j NNP cord-329245-6tj2k1yn 101 104 ) ) -RRB- cord-329245-6tj2k1yn 101 105 and and CC cord-329245-6tj2k1yn 101 106 rabbit rabbit NNP cord-329245-6tj2k1yn 101 107 anti anti NNP cord-329245-6tj2k1yn 101 108 - - JJ cord-329245-6tj2k1yn 101 109 M M NNP cord-329245-6tj2k1yn 101 110 antibody antibody NN cord-329245-6tj2k1yn 101 111 ( ( -LRB- cord-329245-6tj2k1yn 101 112 b b NN cord-329245-6tj2k1yn 101 113 , , , cord-329245-6tj2k1yn 101 114 e e NNP cord-329245-6tj2k1yn 101 115 , , , cord-329245-6tj2k1yn 101 116 h h NNP cord-329245-6tj2k1yn 101 117 , , , cord-329245-6tj2k1yn 101 118 and and CC cord-329245-6tj2k1yn 101 119 k k NNP cord-329245-6tj2k1yn 101 120 ) ) -RRB- cord-329245-6tj2k1yn 101 121 . . . cord-329245-6tj2k1yn 102 1 Secondary secondary JJ cord-329245-6tj2k1yn 102 2 antibodies antibody NNS cord-329245-6tj2k1yn 102 3 were be VBD cord-329245-6tj2k1yn 102 4 fluorescein fluorescein NN cord-329245-6tj2k1yn 102 5 - - HYPH cord-329245-6tj2k1yn 102 6 conjugated conjugate VBN cord-329245-6tj2k1yn 102 7 goat goat NN cord-329245-6tj2k1yn 102 8 anti anti JJ cord-329245-6tj2k1yn 102 9 - - JJ cord-329245-6tj2k1yn 102 10 rat rat JJ cord-329245-6tj2k1yn 102 11 IgG igg NN cord-329245-6tj2k1yn 102 12 and and CC cord-329245-6tj2k1yn 102 13 Texas Texas NNP cord-329245-6tj2k1yn 102 14 red red JJ cord-329245-6tj2k1yn 102 15 conjugated conjugated JJ cord-329245-6tj2k1yn 102 16 donkey donkey NNP cord-329245-6tj2k1yn 102 17 anti anti JJ cord-329245-6tj2k1yn 102 18 - - JJ cord-329245-6tj2k1yn 102 19 rabbit rabbit JJ cord-329245-6tj2k1yn 102 20 IgG. IgG. NNP cord-329245-6tj2k1yn 103 1 The the DT cord-329245-6tj2k1yn 103 2 third third JJ cord-329245-6tj2k1yn 103 3 image image NN cord-329245-6tj2k1yn 103 4 in in IN cord-329245-6tj2k1yn 103 5 each each DT cord-329245-6tj2k1yn 103 6 row row NN cord-329245-6tj2k1yn 103 7 ( ( -LRB- cord-329245-6tj2k1yn 103 8 c c NN cord-329245-6tj2k1yn 103 9 , , , cord-329245-6tj2k1yn 103 10 f f NNP cord-329245-6tj2k1yn 103 11 , , , cord-329245-6tj2k1yn 103 12 i i PRP cord-329245-6tj2k1yn 103 13 , , , cord-329245-6tj2k1yn 103 14 and and CC cord-329245-6tj2k1yn 103 15 l l NNP cord-329245-6tj2k1yn 103 16 ) ) -RRB- cord-329245-6tj2k1yn 103 17 is be VBZ cord-329245-6tj2k1yn 103 18 a a DT cord-329245-6tj2k1yn 103 19 DIC DIC NNP cord-329245-6tj2k1yn 103 20 image image NN cord-329245-6tj2k1yn 103 21 of of IN cord-329245-6tj2k1yn 103 22 the the DT cord-329245-6tj2k1yn 103 23 labeled label VBN cord-329245-6tj2k1yn 103 24 cells cell NNS cord-329245-6tj2k1yn 103 25 . . . cord-329245-6tj2k1yn 104 1 In in IN cord-329245-6tj2k1yn 104 2 the the DT cord-329245-6tj2k1yn 104 3 diagrams diagram NNS cord-329245-6tj2k1yn 104 4 of of IN cord-329245-6tj2k1yn 104 5 the the DT cord-329245-6tj2k1yn 104 6 E e NN cord-329245-6tj2k1yn 104 7 mutant mutant NN cord-329245-6tj2k1yn 104 8 and and CC cord-329245-6tj2k1yn 104 9 chimeric chimeric JJ cord-329245-6tj2k1yn 104 10 proteins protein NNS cord-329245-6tj2k1yn 104 11 , , , cord-329245-6tj2k1yn 104 12 IBV IBV NNP cord-329245-6tj2k1yn 104 13 E E NNP cord-329245-6tj2k1yn 104 14 sequence sequence NN cord-329245-6tj2k1yn 104 15 is be VBZ cord-329245-6tj2k1yn 104 16 shown show VBN cord-329245-6tj2k1yn 104 17 in in IN cord-329245-6tj2k1yn 104 18 black black JJ cord-329245-6tj2k1yn 104 19 and and CC cord-329245-6tj2k1yn 104 20 VSV VSV NNP cord-329245-6tj2k1yn 104 21 G G NNP cord-329245-6tj2k1yn 104 22 sequence sequence NN cord-329245-6tj2k1yn 104 23 is be VBZ cord-329245-6tj2k1yn 104 24 shown show VBN cord-329245-6tj2k1yn 104 25 in in IN cord-329245-6tj2k1yn 104 26 gray gray JJ cord-329245-6tj2k1yn 104 27 . . . cord-329245-6tj2k1yn 105 1 Bar bar NN cord-329245-6tj2k1yn 105 2 , , , cord-329245-6tj2k1yn 105 3 10 10 CD cord-329245-6tj2k1yn 105 4 m. m. NN cord-329245-6tj2k1yn 105 5 ( ( -LRB- cord-329245-6tj2k1yn 105 6 B B NNP cord-329245-6tj2k1yn 105 7 ) ) -RRB- cord-329245-6tj2k1yn 105 8 OST7 OST7 NNP cord-329245-6tj2k1yn 105 9 - - HYPH cord-329245-6tj2k1yn 105 10 1 1 CD cord-329245-6tj2k1yn 105 11 cells cell NNS cord-329245-6tj2k1yn 105 12 expressing express VBG cord-329245-6tj2k1yn 105 13 wild wild JJ cord-329245-6tj2k1yn 105 14 - - HYPH cord-329245-6tj2k1yn 105 15 type type NN cord-329245-6tj2k1yn 105 16 E e NN cord-329245-6tj2k1yn 105 17 protein protein NN cord-329245-6tj2k1yn 105 18 and and CC cord-329245-6tj2k1yn 105 19 Mct Mct NNP cord-329245-6tj2k1yn 105 20 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 105 21 1 1 CD cord-329245-6tj2k1yn 105 22 ( ( -LRB- cord-329245-6tj2k1yn 105 23 a a NNP cord-329245-6tj2k1yn 105 24 - - HYPH cord-329245-6tj2k1yn 105 25 c c NN cord-329245-6tj2k1yn 105 26 ) ) -RRB- cord-329245-6tj2k1yn 105 27 , , , cord-329245-6tj2k1yn 105 28 Mct Mct NNP cord-329245-6tj2k1yn 105 29 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 105 30 2 2 CD cord-329245-6tj2k1yn 105 31 ( ( -LRB- cord-329245-6tj2k1yn 105 32 d d NN cord-329245-6tj2k1yn 105 33 - - HYPH cord-329245-6tj2k1yn 105 34 f f NNP cord-329245-6tj2k1yn 105 35 ) ) -RRB- cord-329245-6tj2k1yn 105 36 , , , cord-329245-6tj2k1yn 105 37 Mct Mct NNP cord-329245-6tj2k1yn 105 38 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 105 39 3 3 CD cord-329245-6tj2k1yn 106 1 ( ( -LRB- cord-329245-6tj2k1yn 106 2 g g NN cord-329245-6tj2k1yn 106 3 - - : cord-329245-6tj2k1yn 106 4 i i NNP cord-329245-6tj2k1yn 106 5 ) ) -RRB- cord-329245-6tj2k1yn 106 6 , , , cord-329245-6tj2k1yn 106 7 or or CC cord-329245-6tj2k1yn 106 8 M M NNP cord-329245-6tj2k1yn 106 9 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 106 10 m2,3 m2,3 NNP cord-329245-6tj2k1yn 106 11 ( ( -LRB- cord-329245-6tj2k1yn 106 12 j j NNP cord-329245-6tj2k1yn 106 13 - - HYPH cord-329245-6tj2k1yn 106 14 l l NNP cord-329245-6tj2k1yn 106 15 ) ) -RRB- cord-329245-6tj2k1yn 106 16 were be VBD cord-329245-6tj2k1yn 106 17 fixed fix VBN cord-329245-6tj2k1yn 106 18 for for IN cord-329245-6tj2k1yn 106 19 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 106 20 and and CC cord-329245-6tj2k1yn 106 21 double double JJ cord-329245-6tj2k1yn 106 22 labeled label VBN cord-329245-6tj2k1yn 106 23 with with IN cord-329245-6tj2k1yn 106 24 rat rat NN cord-329245-6tj2k1yn 106 25 anti anti JJ cord-329245-6tj2k1yn 106 26 - - JJ cord-329245-6tj2k1yn 106 27 E e JJ cord-329245-6tj2k1yn 106 28 antibody antibody NN cord-329245-6tj2k1yn 106 29 ( ( -LRB- cord-329245-6tj2k1yn 106 30 a a DT cord-329245-6tj2k1yn 106 31 , , , cord-329245-6tj2k1yn 106 32 d d NNP cord-329245-6tj2k1yn 106 33 , , , cord-329245-6tj2k1yn 106 34 g g NNP cord-329245-6tj2k1yn 106 35 , , , cord-329245-6tj2k1yn 106 36 and and CC cord-329245-6tj2k1yn 106 37 j j NNP cord-329245-6tj2k1yn 106 38 ) ) -RRB- cord-329245-6tj2k1yn 106 39 and and CC cord-329245-6tj2k1yn 106 40 rabbit rabbit NNP cord-329245-6tj2k1yn 106 41 anti anti NNP cord-329245-6tj2k1yn 106 42 - - JJ cord-329245-6tj2k1yn 106 43 M M NNP cord-329245-6tj2k1yn 106 44 antibody antibody NN cord-329245-6tj2k1yn 106 45 ( ( -LRB- cord-329245-6tj2k1yn 106 46 b b NN cord-329245-6tj2k1yn 106 47 , , , cord-329245-6tj2k1yn 106 48 e e NNP cord-329245-6tj2k1yn 106 49 , , , cord-329245-6tj2k1yn 106 50 h h NNP cord-329245-6tj2k1yn 106 51 , , , cord-329245-6tj2k1yn 106 52 and and CC cord-329245-6tj2k1yn 106 53 k k NNP cord-329245-6tj2k1yn 106 54 ) ) -RRB- cord-329245-6tj2k1yn 106 55 . . . cord-329245-6tj2k1yn 107 1 Secondary secondary JJ cord-329245-6tj2k1yn 107 2 antibodies antibody NNS cord-329245-6tj2k1yn 107 3 were be VBD cord-329245-6tj2k1yn 107 4 fluorescein fluorescein NN cord-329245-6tj2k1yn 107 5 - - HYPH cord-329245-6tj2k1yn 107 6 conjugated conjugate VBN cord-329245-6tj2k1yn 107 7 goat goat NN cord-329245-6tj2k1yn 107 8 anti anti JJ cord-329245-6tj2k1yn 107 9 - - JJ cord-329245-6tj2k1yn 107 10 rat rat JJ cord-329245-6tj2k1yn 107 11 IgG igg NN cord-329245-6tj2k1yn 107 12 and and CC cord-329245-6tj2k1yn 107 13 Texas Texas NNP cord-329245-6tj2k1yn 107 14 red red JJ cord-329245-6tj2k1yn 107 15 conjugated conjugated JJ cord-329245-6tj2k1yn 107 16 donkey donkey NNP cord-329245-6tj2k1yn 107 17 anti anti JJ cord-329245-6tj2k1yn 107 18 - - JJ cord-329245-6tj2k1yn 107 19 rabbit rabbit JJ cord-329245-6tj2k1yn 107 20 IgG. IgG. NNP cord-329245-6tj2k1yn 108 1 The the DT cord-329245-6tj2k1yn 108 2 third third JJ cord-329245-6tj2k1yn 108 3 image image NN cord-329245-6tj2k1yn 108 4 in in IN cord-329245-6tj2k1yn 108 5 each each DT cord-329245-6tj2k1yn 108 6 row row NN cord-329245-6tj2k1yn 108 7 ( ( -LRB- cord-329245-6tj2k1yn 108 8 c c NN cord-329245-6tj2k1yn 108 9 , , , cord-329245-6tj2k1yn 108 10 f f NNP cord-329245-6tj2k1yn 108 11 , , , cord-329245-6tj2k1yn 108 12 i i PRP cord-329245-6tj2k1yn 108 13 , , , cord-329245-6tj2k1yn 108 14 and and CC cord-329245-6tj2k1yn 108 15 l l NNP cord-329245-6tj2k1yn 108 16 ) ) -RRB- cord-329245-6tj2k1yn 108 17 is be VBZ cord-329245-6tj2k1yn 108 18 a a DT cord-329245-6tj2k1yn 108 19 DIC DIC NNP cord-329245-6tj2k1yn 108 20 image image NN cord-329245-6tj2k1yn 108 21 of of IN cord-329245-6tj2k1yn 108 22 the the DT cord-329245-6tj2k1yn 108 23 labeled label VBN cord-329245-6tj2k1yn 108 24 cells cell NNS cord-329245-6tj2k1yn 108 25 . . . cord-329245-6tj2k1yn 109 1 In in IN cord-329245-6tj2k1yn 109 2 the the DT cord-329245-6tj2k1yn 109 3 diagrams diagram NNS cord-329245-6tj2k1yn 109 4 of of IN cord-329245-6tj2k1yn 109 5 the the DT cord-329245-6tj2k1yn 109 6 deletion deletion NN cord-329245-6tj2k1yn 109 7 mutant mutant NN cord-329245-6tj2k1yn 109 8 M M NNP cord-329245-6tj2k1yn 109 9 proteins protein NNS cord-329245-6tj2k1yn 109 10 , , , cord-329245-6tj2k1yn 109 11 the the DT cord-329245-6tj2k1yn 109 12 thin thin JJ cord-329245-6tj2k1yn 109 13 lines line NNS cord-329245-6tj2k1yn 109 14 indicate indicate VBP cord-329245-6tj2k1yn 109 15 deleted delete VBN cord-329245-6tj2k1yn 109 16 sequence sequence NN cord-329245-6tj2k1yn 109 17 . . . cord-329245-6tj2k1yn 110 1 Bar bar NN cord-329245-6tj2k1yn 110 2 , , , cord-329245-6tj2k1yn 110 3 10 10 CD cord-329245-6tj2k1yn 110 4 m. m. NNP cord-329245-6tj2k1yn 110 5 OST7 OST7 NNP cord-329245-6tj2k1yn 110 6 - - HYPH cord-329245-6tj2k1yn 110 7 1 1 CD cord-329245-6tj2k1yn 110 8 cells cell NNS cord-329245-6tj2k1yn 110 9 expressing express VBG cord-329245-6tj2k1yn 110 10 wild wild JJ cord-329245-6tj2k1yn 110 11 - - HYPH cord-329245-6tj2k1yn 110 12 type type NN cord-329245-6tj2k1yn 110 13 M M NNP cord-329245-6tj2k1yn 110 14 protein protein NN cord-329245-6tj2k1yn 110 15 and and CC cord-329245-6tj2k1yn 110 16 wild wild JJ cord-329245-6tj2k1yn 110 17 - - HYPH cord-329245-6tj2k1yn 110 18 type type NN cord-329245-6tj2k1yn 110 19 E e NN cord-329245-6tj2k1yn 110 20 protein protein NN cord-329245-6tj2k1yn 110 21 or or CC cord-329245-6tj2k1yn 110 22 the the DT cord-329245-6tj2k1yn 110 23 E e NN cord-329245-6tj2k1yn 110 24 transmembrane transmembrane NN cord-329245-6tj2k1yn 110 25 replacement replacement NN cord-329245-6tj2k1yn 110 26 mutant mutant NN cord-329245-6tj2k1yn 110 27 proteins protein NNS cord-329245-6tj2k1yn 110 28 EG1 EG1 NNP cord-329245-6tj2k1yn 110 29 , , , cord-329245-6tj2k1yn 110 30 EG2 EG2 NNP cord-329245-6tj2k1yn 110 31 , , , cord-329245-6tj2k1yn 110 32 or or CC cord-329245-6tj2k1yn 110 33 EG3 EG3 NNP cord-329245-6tj2k1yn 110 34 were be VBD cord-329245-6tj2k1yn 110 35 labeled label VBN cord-329245-6tj2k1yn 110 36 with with IN cord-329245-6tj2k1yn 110 37 [ [ -LRB- cord-329245-6tj2k1yn 110 38 35 35 CD cord-329245-6tj2k1yn 110 39 S]methionine S]methionine NNP cord-329245-6tj2k1yn 110 40 - - HYPH cord-329245-6tj2k1yn 110 41 cysteine cysteine NN cord-329245-6tj2k1yn 110 42 , , , cord-329245-6tj2k1yn 110 43 treated treat VBN cord-329245-6tj2k1yn 110 44 with with IN cord-329245-6tj2k1yn 110 45 DSP DSP NNP cord-329245-6tj2k1yn 110 46 , , , cord-329245-6tj2k1yn 110 47 lysed lyse VBN cord-329245-6tj2k1yn 110 48 , , , cord-329245-6tj2k1yn 110 49 and and CC cord-329245-6tj2k1yn 110 50 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 110 51 with with IN cord-329245-6tj2k1yn 110 52 anti anti JJ cord-329245-6tj2k1yn 110 53 - - JJ cord-329245-6tj2k1yn 110 54 E e JJ cord-329245-6tj2k1yn 110 55 or or CC cord-329245-6tj2k1yn 110 56 anti anti JJ cord-329245-6tj2k1yn 110 57 - - JJ cord-329245-6tj2k1yn 110 58 M m JJ cord-329245-6tj2k1yn 110 59 antibodies antibody NNS cord-329245-6tj2k1yn 110 60 as as IN cord-329245-6tj2k1yn 110 61 described describe VBN cord-329245-6tj2k1yn 110 62 under under IN cord-329245-6tj2k1yn 110 63 Materials material NNS cord-329245-6tj2k1yn 110 64 and and CC cord-329245-6tj2k1yn 110 65 methods method NNS cord-329245-6tj2k1yn 110 66 . . . cord-329245-6tj2k1yn 111 1 The the DT cord-329245-6tj2k1yn 111 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 111 3 were be VBD cord-329245-6tj2k1yn 111 4 analyzed analyze VBN cord-329245-6tj2k1yn 111 5 by by IN cord-329245-6tj2k1yn 111 6 SDS-17.5 SDS-17.5 NNP cord-329245-6tj2k1yn 111 7 % % NN cord-329245-6tj2k1yn 111 8 PAGE page NN cord-329245-6tj2k1yn 111 9 in in IN cord-329245-6tj2k1yn 111 10 the the DT cord-329245-6tj2k1yn 111 11 presence presence NN cord-329245-6tj2k1yn 111 12 of of IN cord-329245-6tj2k1yn 111 13 ␤ ␤ NNP cord-329245-6tj2k1yn 111 14 ME ME NNP cord-329245-6tj2k1yn 111 15 , , , cord-329245-6tj2k1yn 111 16 and and CC cord-329245-6tj2k1yn 111 17 fluorography fluorography NNP cord-329245-6tj2k1yn 111 18 . . . cord-329245-6tj2k1yn 112 1 The the DT cord-329245-6tj2k1yn 112 2 asterisk asterisk NN cord-329245-6tj2k1yn 112 3 indicates indicate VBZ cord-329245-6tj2k1yn 112 4 a a DT cord-329245-6tj2k1yn 112 5 band band NN cord-329245-6tj2k1yn 112 6 that that WDT cord-329245-6tj2k1yn 112 7 corresponds correspond VBZ cord-329245-6tj2k1yn 112 8 to to IN cord-329245-6tj2k1yn 112 9 M M NNP cord-329245-6tj2k1yn 112 10 protein protein NN cord-329245-6tj2k1yn 112 11 dimers dimer NNS cord-329245-6tj2k1yn 112 12 . . . cord-329245-6tj2k1yn 113 1 ( ( -LRB- cord-329245-6tj2k1yn 113 2 B b NN cord-329245-6tj2k1yn 113 3 ) ) -RRB- cord-329245-6tj2k1yn 113 4 OST7 OST7 NNP cord-329245-6tj2k1yn 113 5 - - HYPH cord-329245-6tj2k1yn 113 6 1 1 CD cord-329245-6tj2k1yn 113 7 cells cell NNS cord-329245-6tj2k1yn 113 8 coexpressing coexpresse VBG cord-329245-6tj2k1yn 113 9 the the DT cord-329245-6tj2k1yn 113 10 GEt get NN cord-329245-6tj2k1yn 113 11 chimera chimera NN cord-329245-6tj2k1yn 113 12 and and CC cord-329245-6tj2k1yn 113 13 wild wild JJ cord-329245-6tj2k1yn 113 14 - - HYPH cord-329245-6tj2k1yn 113 15 type type NN cord-329245-6tj2k1yn 113 16 M M NNP cord-329245-6tj2k1yn 113 17 protein protein NN cord-329245-6tj2k1yn 113 18 were be VBD cord-329245-6tj2k1yn 113 19 labeled label VBN cord-329245-6tj2k1yn 113 20 with with IN cord-329245-6tj2k1yn 113 21 [ [ -LRB- cord-329245-6tj2k1yn 113 22 35 35 CD cord-329245-6tj2k1yn 113 23 S]methionine S]methionine NNP cord-329245-6tj2k1yn 113 24 - - HYPH cord-329245-6tj2k1yn 113 25 cysteine cysteine NN cord-329245-6tj2k1yn 113 26 , , , cord-329245-6tj2k1yn 113 27 treated treat VBN cord-329245-6tj2k1yn 113 28 with with IN cord-329245-6tj2k1yn 113 29 DSP DSP NNP cord-329245-6tj2k1yn 113 30 , , , cord-329245-6tj2k1yn 113 31 lysed lyse VBN cord-329245-6tj2k1yn 113 32 , , , cord-329245-6tj2k1yn 113 33 and and CC cord-329245-6tj2k1yn 113 34 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 113 35 with with IN cord-329245-6tj2k1yn 113 36 anti anti JJ cord-329245-6tj2k1yn 113 37 - - JJ cord-329245-6tj2k1yn 113 38 E e JJ cord-329245-6tj2k1yn 113 39 or or CC cord-329245-6tj2k1yn 113 40 anti anti JJ cord-329245-6tj2k1yn 113 41 - - JJ cord-329245-6tj2k1yn 113 42 M m JJ cord-329245-6tj2k1yn 113 43 antibodies antibody NNS cord-329245-6tj2k1yn 113 44 as as IN cord-329245-6tj2k1yn 113 45 described describe VBN cord-329245-6tj2k1yn 113 46 under under IN cord-329245-6tj2k1yn 113 47 Materials material NNS cord-329245-6tj2k1yn 113 48 and and CC cord-329245-6tj2k1yn 113 49 methods method NNS cord-329245-6tj2k1yn 113 50 . . . cord-329245-6tj2k1yn 114 1 The the DT cord-329245-6tj2k1yn 114 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 114 3 were be VBD cord-329245-6tj2k1yn 114 4 analyzed analyze VBN cord-329245-6tj2k1yn 114 5 by by IN cord-329245-6tj2k1yn 114 6 SDS-15 sds-15 CD cord-329245-6tj2k1yn 114 7 % % NN cord-329245-6tj2k1yn 114 8 PAGE page NN cord-329245-6tj2k1yn 114 9 in in IN cord-329245-6tj2k1yn 114 10 the the DT cord-329245-6tj2k1yn 114 11 presence presence NN cord-329245-6tj2k1yn 114 12 of of IN cord-329245-6tj2k1yn 114 13 ␤ ␤ NNP cord-329245-6tj2k1yn 114 14 ME ME NNP cord-329245-6tj2k1yn 114 15 , , , cord-329245-6tj2k1yn 114 16 and and CC cord-329245-6tj2k1yn 114 17 fluorography fluorography NNP cord-329245-6tj2k1yn 114 18 . . . cord-329245-6tj2k1yn 115 1 The the DT cord-329245-6tj2k1yn 115 2 asterisk asterisk NN cord-329245-6tj2k1yn 115 3 indicates indicate VBZ cord-329245-6tj2k1yn 115 4 a a DT cord-329245-6tj2k1yn 115 5 band band NN cord-329245-6tj2k1yn 115 6 that that WDT cord-329245-6tj2k1yn 115 7 corresponds correspond VBZ cord-329245-6tj2k1yn 115 8 to to IN cord-329245-6tj2k1yn 115 9 M M NNP cord-329245-6tj2k1yn 115 10 protein protein NN cord-329245-6tj2k1yn 115 11 dimers dimer NNS cord-329245-6tj2k1yn 115 12 . . . cord-329245-6tj2k1yn 116 1 ( ( -LRB- cord-329245-6tj2k1yn 116 2 C C NNP cord-329245-6tj2k1yn 116 3 ) ) -RRB- cord-329245-6tj2k1yn 117 1 OST7 OST7 NNP cord-329245-6tj2k1yn 117 2 - - HYPH cord-329245-6tj2k1yn 117 3 1 1 CD cord-329245-6tj2k1yn 118 1 expressing express VBG cord-329245-6tj2k1yn 118 2 CTE CTE NNP cord-329245-6tj2k1yn 118 3 protein protein NN cord-329245-6tj2k1yn 118 4 alone alone RB cord-329245-6tj2k1yn 118 5 or or CC cord-329245-6tj2k1yn 118 6 with with IN cord-329245-6tj2k1yn 118 7 M M NNP cord-329245-6tj2k1yn 118 8 protein protein NN cord-329245-6tj2k1yn 118 9 were be VBD cord-329245-6tj2k1yn 118 10 labeled label VBN cord-329245-6tj2k1yn 118 11 with with IN cord-329245-6tj2k1yn 118 12 [ [ -LRB- cord-329245-6tj2k1yn 118 13 35 35 CD cord-329245-6tj2k1yn 118 14 S]methionine S]methionine NNP cord-329245-6tj2k1yn 118 15 - - HYPH cord-329245-6tj2k1yn 118 16 cysteine cysteine NN cord-329245-6tj2k1yn 118 17 , , , cord-329245-6tj2k1yn 118 18 treated treat VBN cord-329245-6tj2k1yn 118 19 with with IN cord-329245-6tj2k1yn 118 20 DSP DSP NNP cord-329245-6tj2k1yn 118 21 , , , cord-329245-6tj2k1yn 118 22 lysed lyse VBN cord-329245-6tj2k1yn 118 23 , , , cord-329245-6tj2k1yn 118 24 and and CC cord-329245-6tj2k1yn 118 25 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 118 26 with with IN cord-329245-6tj2k1yn 118 27 anti anti JJ cord-329245-6tj2k1yn 118 28 - - JJ cord-329245-6tj2k1yn 118 29 E e JJ cord-329245-6tj2k1yn 118 30 antibodies antibody NNS cord-329245-6tj2k1yn 118 31 as as IN cord-329245-6tj2k1yn 118 32 described describe VBN cord-329245-6tj2k1yn 118 33 under under IN cord-329245-6tj2k1yn 118 34 Materials material NNS cord-329245-6tj2k1yn 118 35 and and CC cord-329245-6tj2k1yn 118 36 methods method NNS cord-329245-6tj2k1yn 118 37 . . . cord-329245-6tj2k1yn 119 1 The the DT cord-329245-6tj2k1yn 119 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 119 3 were be VBD cord-329245-6tj2k1yn 119 4 analyzed analyze VBN cord-329245-6tj2k1yn 119 5 by by IN cord-329245-6tj2k1yn 119 6 SDS-17.5 SDS-17.5 NNP cord-329245-6tj2k1yn 119 7 % % NN cord-329245-6tj2k1yn 119 8 PAGE page NN cord-329245-6tj2k1yn 119 9 in in IN cord-329245-6tj2k1yn 119 10 the the DT cord-329245-6tj2k1yn 119 11 presence presence NN cord-329245-6tj2k1yn 119 12 of of IN cord-329245-6tj2k1yn 119 13 ␤ ␤ NNP cord-329245-6tj2k1yn 119 14 ME ME NNP cord-329245-6tj2k1yn 119 15 , , , cord-329245-6tj2k1yn 119 16 and and CC cord-329245-6tj2k1yn 119 17 fluorography fluorography NNP cord-329245-6tj2k1yn 119 18 . . . cord-329245-6tj2k1yn 120 1 These these DT cord-329245-6tj2k1yn 120 2 data datum NNS cord-329245-6tj2k1yn 120 3 are be VBP cord-329245-6tj2k1yn 120 4 representative representative JJ cord-329245-6tj2k1yn 120 5 of of IN cord-329245-6tj2k1yn 120 6 at at RB cord-329245-6tj2k1yn 120 7 least least RBS cord-329245-6tj2k1yn 120 8 three three CD cord-329245-6tj2k1yn 120 9 independent independent JJ cord-329245-6tj2k1yn 120 10 experiments experiment NNS cord-329245-6tj2k1yn 120 11 . . . cord-329245-6tj2k1yn 121 1 the the DT cord-329245-6tj2k1yn 121 2 E e NN cord-329245-6tj2k1yn 121 3 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 121 4 tail tail NN cord-329245-6tj2k1yn 121 5 and and CC cord-329245-6tj2k1yn 121 6 thus thus RB cord-329245-6tj2k1yn 121 7 the the DT cord-329245-6tj2k1yn 121 8 GEt get NN cord-329245-6tj2k1yn 121 9 chimera chimera NN cord-329245-6tj2k1yn 121 10 . . . cord-329245-6tj2k1yn 122 1 The the DT cord-329245-6tj2k1yn 122 2 reciprocal reciprocal JJ cord-329245-6tj2k1yn 122 3 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 122 4 with with IN cord-329245-6tj2k1yn 122 5 anti anti JJ cord-329245-6tj2k1yn 122 6 - - JJ cord-329245-6tj2k1yn 122 7 M m JJ cord-329245-6tj2k1yn 122 8 antibody antibody NN cord-329245-6tj2k1yn 122 9 contained contain VBD cord-329245-6tj2k1yn 122 10 GEt get VBP cord-329245-6tj2k1yn 122 11 protein protein NN cord-329245-6tj2k1yn 122 12 ( ( -LRB- cord-329245-6tj2k1yn 122 13 lane lane NN cord-329245-6tj2k1yn 122 14 3 3 CD cord-329245-6tj2k1yn 122 15 ) ) -RRB- cord-329245-6tj2k1yn 122 16 . . . cord-329245-6tj2k1yn 123 1 We -PRON- PRP cord-329245-6tj2k1yn 123 2 conclude conclude VBP cord-329245-6tj2k1yn 123 3 that that IN cord-329245-6tj2k1yn 123 4 the the DT cord-329245-6tj2k1yn 123 5 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 123 6 tail tail NN cord-329245-6tj2k1yn 123 7 of of IN cord-329245-6tj2k1yn 123 8 IBV IBV NNP cord-329245-6tj2k1yn 123 9 E E NNP cord-329245-6tj2k1yn 123 10 protein protein NN cord-329245-6tj2k1yn 123 11 is be VBZ cord-329245-6tj2k1yn 123 12 sufficient sufficient JJ cord-329245-6tj2k1yn 123 13 for for IN cord-329245-6tj2k1yn 123 14 interaction interaction NN cord-329245-6tj2k1yn 123 15 with with IN cord-329245-6tj2k1yn 123 16 M M NNP cord-329245-6tj2k1yn 123 17 protein protein NN cord-329245-6tj2k1yn 123 18 . . . cord-329245-6tj2k1yn 124 1 Indeed indeed RB cord-329245-6tj2k1yn 124 2 , , , cord-329245-6tj2k1yn 124 3 the the DT cord-329245-6tj2k1yn 124 4 N n CD cord-329245-6tj2k1yn 124 5 - - HYPH cord-329245-6tj2k1yn 124 6 terminal terminal NN cord-329245-6tj2k1yn 124 7 truncation truncation NN cord-329245-6tj2k1yn 124 8 mutant mutant JJ cord-329245-6tj2k1yn 124 9 CTE CTE NNP cord-329245-6tj2k1yn 124 10 , , , cord-329245-6tj2k1yn 124 11 which which WDT cord-329245-6tj2k1yn 124 12 consists consist VBZ cord-329245-6tj2k1yn 124 13 only only RB cord-329245-6tj2k1yn 124 14 of of IN cord-329245-6tj2k1yn 124 15 the the DT cord-329245-6tj2k1yn 124 16 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 124 17 tail tail NN cord-329245-6tj2k1yn 124 18 of of IN cord-329245-6tj2k1yn 124 19 IBV IBV NNP cord-329245-6tj2k1yn 124 20 E E NNP cord-329245-6tj2k1yn 124 21 ( ( -LRB- cord-329245-6tj2k1yn 124 22 Corse Corse NNP cord-329245-6tj2k1yn 124 23 and and CC cord-329245-6tj2k1yn 124 24 Machamer Machamer NNP cord-329245-6tj2k1yn 124 25 , , , cord-329245-6tj2k1yn 124 26 2002 2002 CD cord-329245-6tj2k1yn 124 27 ) ) -RRB- cord-329245-6tj2k1yn 124 28 , , , cord-329245-6tj2k1yn 124 29 was be VBD cord-329245-6tj2k1yn 124 30 coprecipitated coprecipitate VBN cord-329245-6tj2k1yn 124 31 with with IN cord-329245-6tj2k1yn 124 32 M M NNP cord-329245-6tj2k1yn 124 33 after after IN cord-329245-6tj2k1yn 124 34 crosslinking crosslinke VBG cord-329245-6tj2k1yn 125 1 ( ( -LRB- cord-329245-6tj2k1yn 125 2 Fig Fig NNP cord-329245-6tj2k1yn 125 3 . . . cord-329245-6tj2k1yn 125 4 4C 4c CD cord-329245-6tj2k1yn 125 5 , , , cord-329245-6tj2k1yn 125 6 lane lane NN cord-329245-6tj2k1yn 125 7 2 2 CD cord-329245-6tj2k1yn 125 8 ) ) -RRB- cord-329245-6tj2k1yn 125 9 . . . cord-329245-6tj2k1yn 126 1 We -PRON- PRP cord-329245-6tj2k1yn 126 2 previously previously RB cord-329245-6tj2k1yn 126 3 found find VBD cord-329245-6tj2k1yn 126 4 that that IN cord-329245-6tj2k1yn 126 5 partial partial JJ cord-329245-6tj2k1yn 126 6 or or CC cord-329245-6tj2k1yn 126 7 complete complete JJ cord-329245-6tj2k1yn 126 8 removal removal NN cord-329245-6tj2k1yn 126 9 of of IN cord-329245-6tj2k1yn 126 10 a a DT cord-329245-6tj2k1yn 126 11 50 50 CD cord-329245-6tj2k1yn 126 12 amino amino NN cord-329245-6tj2k1yn 126 13 acid acid NN cord-329245-6tj2k1yn 126 14 region region NN cord-329245-6tj2k1yn 126 15 within within IN cord-329245-6tj2k1yn 126 16 the the DT cord-329245-6tj2k1yn 126 17 80 80 CD cord-329245-6tj2k1yn 126 18 amino amino NN cord-329245-6tj2k1yn 126 19 acid acid NN cord-329245-6tj2k1yn 126 20 E e NN cord-329245-6tj2k1yn 126 21 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 126 22 tail tail NN cord-329245-6tj2k1yn 126 23 resulted result VBD cord-329245-6tj2k1yn 126 24 in in IN cord-329245-6tj2k1yn 126 25 transport transport NN cord-329245-6tj2k1yn 126 26 of of IN cord-329245-6tj2k1yn 126 27 the the DT cord-329245-6tj2k1yn 126 28 GEt GEt NNS cord-329245-6tj2k1yn 126 29 chimera chimera NN cord-329245-6tj2k1yn 126 30 to to IN cord-329245-6tj2k1yn 126 31 the the DT cord-329245-6tj2k1yn 126 32 cell cell NN cord-329245-6tj2k1yn 126 33 surface surface NN cord-329245-6tj2k1yn 126 34 ( ( -LRB- cord-329245-6tj2k1yn 126 35 Corse Corse NNP cord-329245-6tj2k1yn 126 36 and and CC cord-329245-6tj2k1yn 126 37 Machamer Machamer NNP cord-329245-6tj2k1yn 126 38 , , , cord-329245-6tj2k1yn 126 39 2002 2002 CD cord-329245-6tj2k1yn 126 40 ) ) -RRB- cord-329245-6tj2k1yn 126 41 . . . cord-329245-6tj2k1yn 127 1 Therefore therefore RB cord-329245-6tj2k1yn 127 2 , , , cord-329245-6tj2k1yn 127 3 we -PRON- PRP cord-329245-6tj2k1yn 127 4 did do VBD cord-329245-6tj2k1yn 127 5 not not RB cord-329245-6tj2k1yn 127 6 attempt attempt VB cord-329245-6tj2k1yn 127 7 to to TO cord-329245-6tj2k1yn 127 8 find find VB cord-329245-6tj2k1yn 127 9 a a DT cord-329245-6tj2k1yn 127 10 specific specific JJ cord-329245-6tj2k1yn 127 11 region region NN cord-329245-6tj2k1yn 127 12 within within IN cord-329245-6tj2k1yn 127 13 the the DT cord-329245-6tj2k1yn 127 14 IBV IBV NNP cord-329245-6tj2k1yn 127 15 E e NN cord-329245-6tj2k1yn 127 16 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 127 17 tail tail NN cord-329245-6tj2k1yn 127 18 that that WDT cord-329245-6tj2k1yn 127 19 interacts interact VBZ cord-329245-6tj2k1yn 127 20 with with IN cord-329245-6tj2k1yn 127 21 IBV IBV NNP cord-329245-6tj2k1yn 127 22 M M NNP cord-329245-6tj2k1yn 127 23 , , , cord-329245-6tj2k1yn 127 24 because because IN cord-329245-6tj2k1yn 127 25 colocalization colocalization NN cord-329245-6tj2k1yn 127 26 of of IN cord-329245-6tj2k1yn 127 27 the the DT cord-329245-6tj2k1yn 127 28 IBV IBV NNP cord-329245-6tj2k1yn 127 29 E E NNP cord-329245-6tj2k1yn 127 30 and and CC cord-329245-6tj2k1yn 127 31 M M NNP cord-329245-6tj2k1yn 127 32 proteins protein NNS cord-329245-6tj2k1yn 127 33 is be VBZ cord-329245-6tj2k1yn 127 34 likely likely JJ cord-329245-6tj2k1yn 127 35 to to TO cord-329245-6tj2k1yn 127 36 be be VB cord-329245-6tj2k1yn 127 37 required require VBN cord-329245-6tj2k1yn 127 38 for for IN cord-329245-6tj2k1yn 127 39 their -PRON- PRP$ cord-329245-6tj2k1yn 127 40 interaction interaction NN cord-329245-6tj2k1yn 127 41 . . . cord-329245-6tj2k1yn 128 1 To to TO cord-329245-6tj2k1yn 128 2 determine determine VB cord-329245-6tj2k1yn 128 3 which which WDT cord-329245-6tj2k1yn 128 4 regions region NNS cord-329245-6tj2k1yn 128 5 of of IN cord-329245-6tj2k1yn 128 6 the the DT cord-329245-6tj2k1yn 128 7 IBV IBV NNP cord-329245-6tj2k1yn 128 8 M M NNP cord-329245-6tj2k1yn 128 9 protein protein NN cord-329245-6tj2k1yn 128 10 were be VBD cord-329245-6tj2k1yn 128 11 involved involve VBN cord-329245-6tj2k1yn 128 12 in in IN cord-329245-6tj2k1yn 128 13 interaction interaction NN cord-329245-6tj2k1yn 128 14 with with IN cord-329245-6tj2k1yn 128 15 IBV IBV NNP cord-329245-6tj2k1yn 128 16 E E NNP cord-329245-6tj2k1yn 128 17 , , , cord-329245-6tj2k1yn 128 18 we -PRON- PRP cord-329245-6tj2k1yn 128 19 tested test VBD cord-329245-6tj2k1yn 128 20 how how WRB cord-329245-6tj2k1yn 128 21 well well RB cord-329245-6tj2k1yn 128 22 the the DT cord-329245-6tj2k1yn 128 23 M M NNP cord-329245-6tj2k1yn 128 24 deletion deletion NN cord-329245-6tj2k1yn 128 25 mutants mutant NNS cord-329245-6tj2k1yn 128 26 Mct mct VBP cord-329245-6tj2k1yn 128 27 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 128 28 1 1 CD cord-329245-6tj2k1yn 128 29 , , , cord-329245-6tj2k1yn 128 30 Mct Mct NNP cord-329245-6tj2k1yn 128 31 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 128 32 2 2 CD cord-329245-6tj2k1yn 128 33 , , , cord-329245-6tj2k1yn 128 34 Mct Mct NNP cord-329245-6tj2k1yn 128 35 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 128 36 3 3 CD cord-329245-6tj2k1yn 128 37 , , , cord-329245-6tj2k1yn 128 38 and and CC cord-329245-6tj2k1yn 128 39 M M NNP cord-329245-6tj2k1yn 128 40 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 128 41 m2,3 m2,3 NNP cord-329245-6tj2k1yn 128 42 could could MD cord-329245-6tj2k1yn 128 43 be be VB cord-329245-6tj2k1yn 128 44 crosslinked crosslinke VBN cord-329245-6tj2k1yn 128 45 to to IN cord-329245-6tj2k1yn 128 46 wild wild JJ cord-329245-6tj2k1yn 128 47 - - HYPH cord-329245-6tj2k1yn 128 48 type type NN cord-329245-6tj2k1yn 128 49 E e NN cord-329245-6tj2k1yn 128 50 protein protein NN cord-329245-6tj2k1yn 128 51 compared compare VBN cord-329245-6tj2k1yn 128 52 to to IN cord-329245-6tj2k1yn 128 53 wild wild JJ cord-329245-6tj2k1yn 128 54 - - HYPH cord-329245-6tj2k1yn 128 55 type type NN cord-329245-6tj2k1yn 128 56 M m NN cord-329245-6tj2k1yn 128 57 protein protein NN cord-329245-6tj2k1yn 128 58 ( ( -LRB- cord-329245-6tj2k1yn 128 59 Fig Fig NNP cord-329245-6tj2k1yn 128 60 . . . cord-329245-6tj2k1yn 128 61 5 5 CD cord-329245-6tj2k1yn 128 62 ) ) -RRB- cord-329245-6tj2k1yn 128 63 . . . cord-329245-6tj2k1yn 129 1 M M NNP cord-329245-6tj2k1yn 129 2 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 129 3 m2,3 m2,3 NNP cord-329245-6tj2k1yn 129 4 is be VBZ cord-329245-6tj2k1yn 129 5 missing miss VBG cord-329245-6tj2k1yn 129 6 the the DT cord-329245-6tj2k1yn 129 7 second second JJ cord-329245-6tj2k1yn 129 8 and and CC cord-329245-6tj2k1yn 129 9 third third JJ cord-329245-6tj2k1yn 129 10 transmembrane transmembrane NN cord-329245-6tj2k1yn 129 11 domains domain NNS cord-329245-6tj2k1yn 129 12 of of IN cord-329245-6tj2k1yn 129 13 IBV IBV NNP cord-329245-6tj2k1yn 129 14 M M NNP cord-329245-6tj2k1yn 129 15 ; ; : cord-329245-6tj2k1yn 129 16 since since IN cord-329245-6tj2k1yn 129 17 it -PRON- PRP cord-329245-6tj2k1yn 129 18 was be VBD cord-329245-6tj2k1yn 129 19 crosslinked crosslinke VBN cord-329245-6tj2k1yn 129 20 to to IN cord-329245-6tj2k1yn 129 21 E e NN cord-329245-6tj2k1yn 129 22 protein protein NN cord-329245-6tj2k1yn 129 23 as as RB cord-329245-6tj2k1yn 129 24 well well RB cord-329245-6tj2k1yn 129 25 as as IN cord-329245-6tj2k1yn 129 26 wild wild JJ cord-329245-6tj2k1yn 129 27 - - HYPH cord-329245-6tj2k1yn 129 28 type type NN cord-329245-6tj2k1yn 129 29 M M NNP cord-329245-6tj2k1yn 129 30 ( ( -LRB- cord-329245-6tj2k1yn 129 31 lanes lane NNS cord-329245-6tj2k1yn 129 32 5 5 CD cord-329245-6tj2k1yn 129 33 and and CC cord-329245-6tj2k1yn 129 34 10 10 CD cord-329245-6tj2k1yn 129 35 ) ) -RRB- cord-329245-6tj2k1yn 129 36 , , , cord-329245-6tj2k1yn 129 37 we -PRON- PRP cord-329245-6tj2k1yn 129 38 conclude conclude VBP cord-329245-6tj2k1yn 129 39 that that IN cord-329245-6tj2k1yn 129 40 these these DT cord-329245-6tj2k1yn 129 41 membrane membrane NN cord-329245-6tj2k1yn 129 42 - - HYPH cord-329245-6tj2k1yn 129 43 spanning span VBG cord-329245-6tj2k1yn 129 44 regions region NNS cord-329245-6tj2k1yn 129 45 are be VBP cord-329245-6tj2k1yn 129 46 not not RB cord-329245-6tj2k1yn 129 47 involved involve VBN cord-329245-6tj2k1yn 129 48 in in IN cord-329245-6tj2k1yn 129 49 interactions interaction NNS cord-329245-6tj2k1yn 129 50 with with IN cord-329245-6tj2k1yn 129 51 E. E. NNP cord-329245-6tj2k1yn 129 52 A a DT cord-329245-6tj2k1yn 129 53 large large JJ cord-329245-6tj2k1yn 129 54 portion portion NN cord-329245-6tj2k1yn 129 55 of of IN cord-329245-6tj2k1yn 129 56 the the DT cord-329245-6tj2k1yn 129 57 M M NNP cord-329245-6tj2k1yn 129 58 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 129 59 tail tail NN cord-329245-6tj2k1yn 129 60 is be VBZ cord-329245-6tj2k1yn 129 61 deleted delete VBN cord-329245-6tj2k1yn 129 62 in in IN cord-329245-6tj2k1yn 129 63 Mct Mct NNP cord-329245-6tj2k1yn 129 64 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 129 65 1 1 CD cord-329245-6tj2k1yn 129 66 ( ( -LRB- cord-329245-6tj2k1yn 129 67 see see VB cord-329245-6tj2k1yn 129 68 Fig Fig NNP cord-329245-6tj2k1yn 129 69 . . . cord-329245-6tj2k1yn 129 70 3B 3B NNP cord-329245-6tj2k1yn 129 71 ) ) -RRB- cord-329245-6tj2k1yn 129 72 , , , cord-329245-6tj2k1yn 129 73 and and CC cord-329245-6tj2k1yn 129 74 this this DT cord-329245-6tj2k1yn 129 75 protein protein NN cord-329245-6tj2k1yn 129 76 was be VBD cord-329245-6tj2k1yn 129 77 not not RB cord-329245-6tj2k1yn 129 78 crosslinked crosslinke VBN cord-329245-6tj2k1yn 129 79 efficiently efficiently RB cord-329245-6tj2k1yn 129 80 to to IN cord-329245-6tj2k1yn 129 81 E e NN cord-329245-6tj2k1yn 129 82 , , , cord-329245-6tj2k1yn 129 83 compared compare VBN cord-329245-6tj2k1yn 129 84 to to IN cord-329245-6tj2k1yn 129 85 wild wild JJ cord-329245-6tj2k1yn 129 86 - - HYPH cord-329245-6tj2k1yn 129 87 type type NN cord-329245-6tj2k1yn 129 88 M m NN cord-329245-6tj2k1yn 129 89 protein protein NN cord-329245-6tj2k1yn 129 90 ( ( -LRB- cord-329245-6tj2k1yn 129 91 Fig Fig NNP cord-329245-6tj2k1yn 129 92 . . NNP cord-329245-6tj2k1yn 129 93 5 5 CD cord-329245-6tj2k1yn 129 94 , , , cord-329245-6tj2k1yn 129 95 lanes lane NNS cord-329245-6tj2k1yn 129 96 2 2 CD cord-329245-6tj2k1yn 129 97 and and CC cord-329245-6tj2k1yn 129 98 7 7 CD cord-329245-6tj2k1yn 129 99 ) ) -RRB- cord-329245-6tj2k1yn 129 100 , , , cord-329245-6tj2k1yn 129 101 although although IN cord-329245-6tj2k1yn 129 102 a a DT cord-329245-6tj2k1yn 129 103 small small JJ cord-329245-6tj2k1yn 129 104 amount amount NN cord-329245-6tj2k1yn 129 105 of of IN cord-329245-6tj2k1yn 129 106 E e NN cord-329245-6tj2k1yn 129 107 protein protein NN cord-329245-6tj2k1yn 129 108 is be VBZ cord-329245-6tj2k1yn 129 109 visible visible JJ cord-329245-6tj2k1yn 129 110 in in IN cord-329245-6tj2k1yn 129 111 the the DT cord-329245-6tj2k1yn 129 112 Mct Mct NNP cord-329245-6tj2k1yn 129 113 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 129 114 1 1 CD cord-329245-6tj2k1yn 129 115 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 129 116 ( ( -LRB- cord-329245-6tj2k1yn 129 117 Fig Fig NNP cord-329245-6tj2k1yn 129 118 . . NNP cord-329245-6tj2k1yn 129 119 5 5 CD cord-329245-6tj2k1yn 129 120 , , , cord-329245-6tj2k1yn 129 121 lane lane NN cord-329245-6tj2k1yn 129 122 7 7 CD cord-329245-6tj2k1yn 129 123 ) ) -RRB- cord-329245-6tj2k1yn 129 124 . . . cord-329245-6tj2k1yn 130 1 This this DT cord-329245-6tj2k1yn 130 2 result result NN cord-329245-6tj2k1yn 130 3 indicates indicate VBZ cord-329245-6tj2k1yn 130 4 that that IN cord-329245-6tj2k1yn 130 5 the the DT cord-329245-6tj2k1yn 130 6 M M NNP cord-329245-6tj2k1yn 130 7 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 130 8 tail tail NN cord-329245-6tj2k1yn 130 9 is be VBZ cord-329245-6tj2k1yn 130 10 involved involve VBN cord-329245-6tj2k1yn 130 11 in in IN cord-329245-6tj2k1yn 130 12 interaction interaction NN cord-329245-6tj2k1yn 130 13 with with IN cord-329245-6tj2k1yn 130 14 E. E. NNP cord-329245-6tj2k1yn 131 1 The the DT cord-329245-6tj2k1yn 131 2 portion portion NN cord-329245-6tj2k1yn 131 3 of of IN cord-329245-6tj2k1yn 131 4 the the DT cord-329245-6tj2k1yn 131 5 M M NNP cord-329245-6tj2k1yn 131 6 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 131 7 tail tail NN cord-329245-6tj2k1yn 131 8 removed remove VBN cord-329245-6tj2k1yn 131 9 by by IN cord-329245-6tj2k1yn 131 10 the the DT cord-329245-6tj2k1yn 131 11 Mct Mct NNP cord-329245-6tj2k1yn 131 12 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 131 13 1 1 CD cord-329245-6tj2k1yn 131 14 deletion deletion NN cord-329245-6tj2k1yn 131 15 is be VBZ cord-329245-6tj2k1yn 131 16 split split VBN cord-329245-6tj2k1yn 131 17 into into IN cord-329245-6tj2k1yn 131 18 parts part NNS cord-329245-6tj2k1yn 131 19 by by IN cord-329245-6tj2k1yn 131 20 the the DT cord-329245-6tj2k1yn 131 21 Mct Mct NNP cord-329245-6tj2k1yn 131 22 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 131 23 2 2 CD cord-329245-6tj2k1yn 131 24 and and CC cord-329245-6tj2k1yn 131 25 Mct Mct NNP cord-329245-6tj2k1yn 131 26 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 131 27 3 3 CD cord-329245-6tj2k1yn 131 28 deletions deletion NNS cord-329245-6tj2k1yn 131 29 . . . cord-329245-6tj2k1yn 132 1 Note note VB cord-329245-6tj2k1yn 132 2 that that IN cord-329245-6tj2k1yn 132 3 the the DT cord-329245-6tj2k1yn 132 4 Mct Mct NNP cord-329245-6tj2k1yn 132 5 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 132 6 1 1 CD cord-329245-6tj2k1yn 132 7 and and CC cord-329245-6tj2k1yn 132 8 Mct Mct NNP cord-329245-6tj2k1yn 132 9 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 132 10 2 2 CD cord-329245-6tj2k1yn 132 11 mutants mutant NNS cord-329245-6tj2k1yn 132 12 are be VBP cord-329245-6tj2k1yn 132 13 not not RB cord-329245-6tj2k1yn 132 14 radiolabeled radiolabele VBN cord-329245-6tj2k1yn 132 15 as as RB cord-329245-6tj2k1yn 132 16 efficiently efficiently RB cord-329245-6tj2k1yn 132 17 as as IN cord-329245-6tj2k1yn 132 18 wild wild JJ cord-329245-6tj2k1yn 132 19 - - HYPH cord-329245-6tj2k1yn 132 20 type type NN cord-329245-6tj2k1yn 132 21 M M NNP cord-329245-6tj2k1yn 132 22 and and CC cord-329245-6tj2k1yn 132 23 the the DT cord-329245-6tj2k1yn 132 24 Mct Mct NNP cord-329245-6tj2k1yn 132 25 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 132 26 3 3 CD cord-329245-6tj2k1yn 132 27 and and CC cord-329245-6tj2k1yn 132 28 M M NNP cord-329245-6tj2k1yn 132 29 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 132 30 m2,3 m2,3 NNP cord-329245-6tj2k1yn 132 31 mutants mutant NNS cord-329245-6tj2k1yn 132 32 ( ( -LRB- cord-329245-6tj2k1yn 132 33 compare compare VB cord-329245-6tj2k1yn 132 34 lanes lane NNS cord-329245-6tj2k1yn 132 35 7 7 CD cord-329245-6tj2k1yn 132 36 and and CC cord-329245-6tj2k1yn 132 37 8 8 CD cord-329245-6tj2k1yn 132 38 with with IN cord-329245-6tj2k1yn 132 39 lane lane NN cord-329245-6tj2k1yn 132 40 6 6 CD cord-329245-6tj2k1yn 132 41 ) ) -RRB- cord-329245-6tj2k1yn 132 42 because because IN cord-329245-6tj2k1yn 132 43 they -PRON- PRP cord-329245-6tj2k1yn 132 44 have have VBP cord-329245-6tj2k1yn 132 45 fewer few JJR cord-329245-6tj2k1yn 132 46 methionines methionine NNS cord-329245-6tj2k1yn 132 47 and and CC cord-329245-6tj2k1yn 132 48 cysteines cysteine NNS cord-329245-6tj2k1yn 132 49 due due IN cord-329245-6tj2k1yn 132 50 to to IN cord-329245-6tj2k1yn 132 51 their -PRON- PRP$ cord-329245-6tj2k1yn 132 52 deletions deletion NNS cord-329245-6tj2k1yn 132 53 , , , cord-329245-6tj2k1yn 132 54 which which WDT cord-329245-6tj2k1yn 132 55 could could MD cord-329245-6tj2k1yn 132 56 explain explain VB cord-329245-6tj2k1yn 132 57 why why WRB cord-329245-6tj2k1yn 132 58 a a DT cord-329245-6tj2k1yn 132 59 reciprocal reciprocal JJ cord-329245-6tj2k1yn 132 60 amount amount NN cord-329245-6tj2k1yn 132 61 of of IN cord-329245-6tj2k1yn 132 62 Mct Mct NNP cord-329245-6tj2k1yn 132 63 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 132 64 1 1 CD cord-329245-6tj2k1yn 132 65 is be VBZ cord-329245-6tj2k1yn 132 66 not not RB cord-329245-6tj2k1yn 132 67 seen see VBN cord-329245-6tj2k1yn 132 68 in in IN cord-329245-6tj2k1yn 132 69 the the DT cord-329245-6tj2k1yn 132 70 anti anti JJ cord-329245-6tj2k1yn 132 71 - - JJ cord-329245-6tj2k1yn 132 72 E e JJ cord-329245-6tj2k1yn 132 73 immunoprecipitation immunoprecipitation NN cord-329245-6tj2k1yn 132 74 ( ( -LRB- cord-329245-6tj2k1yn 132 75 Fig Fig NNP cord-329245-6tj2k1yn 132 76 . . NNP cord-329245-6tj2k1yn 132 77 5 5 CD cord-329245-6tj2k1yn 132 78 , , , cord-329245-6tj2k1yn 132 79 _SP cord-329245-6tj2k1yn 132 80 lane lane NNP cord-329245-6tj2k1yn 132 81 2 2 CD cord-329245-6tj2k1yn 132 82 ) ) -RRB- cord-329245-6tj2k1yn 132 83 . . . cord-329245-6tj2k1yn 133 1 Mct Mct NNP cord-329245-6tj2k1yn 133 2 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 133 3 3 3 CD cord-329245-6tj2k1yn 133 4 , , , cord-329245-6tj2k1yn 133 5 which which WDT cord-329245-6tj2k1yn 133 6 is be VBZ cord-329245-6tj2k1yn 133 7 missing miss VBG cord-329245-6tj2k1yn 133 8 amino amino JJ cord-329245-6tj2k1yn 133 9 acids acid NNS cord-329245-6tj2k1yn 133 10 103 103 CD cord-329245-6tj2k1yn 133 11 - - SYM cord-329245-6tj2k1yn 133 12 118 118 CD cord-329245-6tj2k1yn 133 13 of of IN cord-329245-6tj2k1yn 133 14 the the DT cord-329245-6tj2k1yn 133 15 M M NNP cord-329245-6tj2k1yn 133 16 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 133 17 tail tail NN cord-329245-6tj2k1yn 133 18 , , , cord-329245-6tj2k1yn 133 19 was be VBD cord-329245-6tj2k1yn 133 20 crosslinked crosslinke VBN cord-329245-6tj2k1yn 133 21 to to IN cord-329245-6tj2k1yn 133 22 E E NNP cord-329245-6tj2k1yn 133 23 ( ( -LRB- cord-329245-6tj2k1yn 133 24 Fig Fig NNP cord-329245-6tj2k1yn 133 25 . . NNP cord-329245-6tj2k1yn 133 26 5 5 CD cord-329245-6tj2k1yn 133 27 , , , cord-329245-6tj2k1yn 133 28 lanes lane NNS cord-329245-6tj2k1yn 133 29 4 4 CD cord-329245-6tj2k1yn 133 30 and and CC cord-329245-6tj2k1yn 133 31 9 9 CD cord-329245-6tj2k1yn 133 32 ) ) -RRB- cord-329245-6tj2k1yn 133 33 , , , cord-329245-6tj2k1yn 133 34 while while IN cord-329245-6tj2k1yn 133 35 Mct Mct NNP cord-329245-6tj2k1yn 133 36 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 133 37 2 2 CD cord-329245-6tj2k1yn 133 38 , , , cord-329245-6tj2k1yn 133 39 which which WDT cord-329245-6tj2k1yn 133 40 is be VBZ cord-329245-6tj2k1yn 133 41 missing miss VBG cord-329245-6tj2k1yn 133 42 amino amino JJ cord-329245-6tj2k1yn 133 43 acids acid NNS cord-329245-6tj2k1yn 133 44 119 119 CD cord-329245-6tj2k1yn 133 45 -203 -203 NN cord-329245-6tj2k1yn 133 46 of of IN cord-329245-6tj2k1yn 133 47 the the DT cord-329245-6tj2k1yn 133 48 M M NNP cord-329245-6tj2k1yn 133 49 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 133 50 tail tail NN cord-329245-6tj2k1yn 133 51 ( ( -LRB- cord-329245-6tj2k1yn 133 52 see see VB cord-329245-6tj2k1yn 133 53 Fig Fig NNP cord-329245-6tj2k1yn 133 54 . . . cord-329245-6tj2k1yn 133 55 2B 2B NNP cord-329245-6tj2k1yn 133 56 ) ) -RRB- cord-329245-6tj2k1yn 133 57 , , , cord-329245-6tj2k1yn 133 58 was be VBD cord-329245-6tj2k1yn 133 59 not not RB cord-329245-6tj2k1yn 133 60 ( ( -LRB- cord-329245-6tj2k1yn 133 61 Fig Fig NNP cord-329245-6tj2k1yn 133 62 . . NNP cord-329245-6tj2k1yn 133 63 5 5 CD cord-329245-6tj2k1yn 133 64 , , , cord-329245-6tj2k1yn 133 65 lanes lane NNS cord-329245-6tj2k1yn 133 66 3 3 CD cord-329245-6tj2k1yn 133 67 and and CC cord-329245-6tj2k1yn 133 68 8) 8) NN cord-329245-6tj2k1yn 133 69 . . . cord-329245-6tj2k1yn 134 1 This this DT cord-329245-6tj2k1yn 134 2 indicates indicate VBZ cord-329245-6tj2k1yn 134 3 that that IN cord-329245-6tj2k1yn 134 4 the the DT cord-329245-6tj2k1yn 134 5 amino amino NN cord-329245-6tj2k1yn 134 6 acids acid NNS cord-329245-6tj2k1yn 134 7 removed remove VBN cord-329245-6tj2k1yn 134 8 by by IN cord-329245-6tj2k1yn 134 9 the the DT cord-329245-6tj2k1yn 134 10 Mct Mct NNP cord-329245-6tj2k1yn 134 11 ⌬ ⌬ NNS cord-329245-6tj2k1yn 134 12 2 2 CD cord-329245-6tj2k1yn 134 13 deletion deletion NN cord-329245-6tj2k1yn 134 14 ( ( -LRB- cord-329245-6tj2k1yn 134 15 119 119 CD cord-329245-6tj2k1yn 134 16 -203 -203 NN cord-329245-6tj2k1yn 134 17 ) ) -RRB- cord-329245-6tj2k1yn 134 18 are be VBP cord-329245-6tj2k1yn 134 19 required require VBN cord-329245-6tj2k1yn 134 20 for for IN cord-329245-6tj2k1yn 134 21 crosslinking crosslinking NN cord-329245-6tj2k1yn 134 22 of of IN cord-329245-6tj2k1yn 134 23 the the DT cord-329245-6tj2k1yn 134 24 IBV IBV NNP cord-329245-6tj2k1yn 134 25 M M NNP cord-329245-6tj2k1yn 134 26 protein protein NN cord-329245-6tj2k1yn 134 27 to to IN cord-329245-6tj2k1yn 134 28 the the DT cord-329245-6tj2k1yn 134 29 IBV IBV NNP cord-329245-6tj2k1yn 134 30 E E NNP cord-329245-6tj2k1yn 134 31 protein protein NN cord-329245-6tj2k1yn 134 32 . . . cord-329245-6tj2k1yn 135 1 The the DT cord-329245-6tj2k1yn 135 2 E e NN cord-329245-6tj2k1yn 135 3 and and CC cord-329245-6tj2k1yn 135 4 M M NNP cord-329245-6tj2k1yn 135 5 proteins protein NNS cord-329245-6tj2k1yn 135 6 of of IN cord-329245-6tj2k1yn 135 7 several several JJ cord-329245-6tj2k1yn 135 8 coronaviruses coronaviruse NNS cord-329245-6tj2k1yn 135 9 are be VBP cord-329245-6tj2k1yn 135 10 released release VBN cord-329245-6tj2k1yn 135 11 from from IN cord-329245-6tj2k1yn 135 12 cotransfected cotransfected JJ cord-329245-6tj2k1yn 135 13 cells cell NNS cord-329245-6tj2k1yn 135 14 in in IN cord-329245-6tj2k1yn 135 15 membrane membrane NN cord-329245-6tj2k1yn 135 16 - - HYPH cord-329245-6tj2k1yn 135 17 bound bind VBN cord-329245-6tj2k1yn 135 18 particles particle NNS cord-329245-6tj2k1yn 135 19 that that WDT cord-329245-6tj2k1yn 135 20 are be VBP cord-329245-6tj2k1yn 135 21 morphologically morphologically RB cord-329245-6tj2k1yn 135 22 similar similar JJ cord-329245-6tj2k1yn 135 23 to to IN cord-329245-6tj2k1yn 135 24 virions virion NNS cord-329245-6tj2k1yn 135 25 ( ( -LRB- cord-329245-6tj2k1yn 135 26 VLPs VLPs NNP cord-329245-6tj2k1yn 135 27 ) ) -RRB- cord-329245-6tj2k1yn 135 28 , , , cord-329245-6tj2k1yn 135 29 suggesting suggest VBG cord-329245-6tj2k1yn 135 30 that that IN cord-329245-6tj2k1yn 135 31 interactions interaction NNS cord-329245-6tj2k1yn 135 32 between between IN cord-329245-6tj2k1yn 135 33 these these DT cord-329245-6tj2k1yn 135 34 proteins protein NNS cord-329245-6tj2k1yn 135 35 are be VBP cord-329245-6tj2k1yn 135 36 an an DT cord-329245-6tj2k1yn 135 37 integral integral JJ cord-329245-6tj2k1yn 135 38 part part NN cord-329245-6tj2k1yn 135 39 of of IN cord-329245-6tj2k1yn 135 40 coronavirus coronavirus NN cord-329245-6tj2k1yn 135 41 assembly assembly NN cord-329245-6tj2k1yn 136 1 ( ( -LRB- cord-329245-6tj2k1yn 136 2 Baudoux Baudoux NNP cord-329245-6tj2k1yn 136 3 et et NNP cord-329245-6tj2k1yn 136 4 al al NNP cord-329245-6tj2k1yn 136 5 . . NNP cord-329245-6tj2k1yn 136 6 , , , cord-329245-6tj2k1yn 136 7 1998 1998 CD cord-329245-6tj2k1yn 136 8 ; ; : cord-329245-6tj2k1yn 137 1 Corse Corse NNP cord-329245-6tj2k1yn 137 2 and and CC cord-329245-6tj2k1yn 137 3 Machamer Machamer NNP cord-329245-6tj2k1yn 137 4 , , , cord-329245-6tj2k1yn 137 5 2000 2000 CD cord-329245-6tj2k1yn 137 6 ; ; : cord-329245-6tj2k1yn 137 7 Godeke Godeke NNP cord-329245-6tj2k1yn 137 8 et et FW cord-329245-6tj2k1yn 137 9 al al NNP cord-329245-6tj2k1yn 137 10 . . NNP cord-329245-6tj2k1yn 137 11 , , , cord-329245-6tj2k1yn 137 12 2000 2000 CD cord-329245-6tj2k1yn 137 13 ; ; : cord-329245-6tj2k1yn 137 14 Vennema Vennema NNP cord-329245-6tj2k1yn 137 15 et et FW cord-329245-6tj2k1yn 137 16 al al NNP cord-329245-6tj2k1yn 137 17 . . NNP cord-329245-6tj2k1yn 137 18 , , , cord-329245-6tj2k1yn 137 19 1996 1996 CD cord-329245-6tj2k1yn 137 20 ) ) -RRB- cord-329245-6tj2k1yn 137 21 . . . cord-329245-6tj2k1yn 138 1 We -PRON- PRP cord-329245-6tj2k1yn 138 2 initially initially RB cord-329245-6tj2k1yn 138 3 attempted attempt VBD cord-329245-6tj2k1yn 138 4 to to TO cord-329245-6tj2k1yn 138 5 study study VB cord-329245-6tj2k1yn 138 6 VLPs vlp NNS cord-329245-6tj2k1yn 138 7 formed form VBN cord-329245-6tj2k1yn 138 8 in in IN cord-329245-6tj2k1yn 138 9 BHK BHK NNP cord-329245-6tj2k1yn 138 10 cells cell NNS cord-329245-6tj2k1yn 138 11 from from IN cord-329245-6tj2k1yn 138 12 IBV IBV NNP cord-329245-6tj2k1yn 138 13 E E NNP cord-329245-6tj2k1yn 138 14 and and CC cord-329245-6tj2k1yn 138 15 M M NNP cord-329245-6tj2k1yn 138 16 proteins protein NNS cord-329245-6tj2k1yn 138 17 expressed express VBN cord-329245-6tj2k1yn 138 18 by by IN cord-329245-6tj2k1yn 138 19 coinfection coinfection NN cord-329245-6tj2k1yn 138 20 with with IN cord-329245-6tj2k1yn 138 21 recombinant recombinant JJ cord-329245-6tj2k1yn 138 22 vaccinia vaccinia NN cord-329245-6tj2k1yn 138 23 viruses virus NNS cord-329245-6tj2k1yn 138 24 encoding encode VBG cord-329245-6tj2k1yn 138 25 each each DT cord-329245-6tj2k1yn 138 26 protein protein NN cord-329245-6tj2k1yn 138 27 . . . cord-329245-6tj2k1yn 139 1 However however RB cord-329245-6tj2k1yn 139 2 , , , cord-329245-6tj2k1yn 139 3 we -PRON- PRP cord-329245-6tj2k1yn 139 4 found find VBD cord-329245-6tj2k1yn 139 5 that that IN cord-329245-6tj2k1yn 139 6 VLP VLP NNP cord-329245-6tj2k1yn 139 7 formation formation NN cord-329245-6tj2k1yn 139 8 was be VBD cord-329245-6tj2k1yn 139 9 extremely extremely RB cord-329245-6tj2k1yn 139 10 inefficient inefficient JJ cord-329245-6tj2k1yn 139 11 under under IN cord-329245-6tj2k1yn 139 12 these these DT cord-329245-6tj2k1yn 139 13 conditions condition NNS cord-329245-6tj2k1yn 139 14 and and CC cord-329245-6tj2k1yn 139 15 estimated estimate VBD cord-329245-6tj2k1yn 139 16 that that IN cord-329245-6tj2k1yn 139 17 only only RB cord-329245-6tj2k1yn 139 18 0.01 0.01 CD cord-329245-6tj2k1yn 139 19 % % NN cord-329245-6tj2k1yn 139 20 of of IN cord-329245-6tj2k1yn 139 21 the the DT cord-329245-6tj2k1yn 139 22 cellular cellular JJ cord-329245-6tj2k1yn 139 23 E e NN cord-329245-6tj2k1yn 139 24 and and CC cord-329245-6tj2k1yn 139 25 M M NNP cord-329245-6tj2k1yn 139 26 proteins protein NNS cord-329245-6tj2k1yn 139 27 were be VBD cord-329245-6tj2k1yn 139 28 released release VBN cord-329245-6tj2k1yn 139 29 into into IN cord-329245-6tj2k1yn 139 30 the the DT cord-329245-6tj2k1yn 139 31 supernatant supernatant NN cord-329245-6tj2k1yn 139 32 as as IN cord-329245-6tj2k1yn 139 33 VLPs vlp NNS cord-329245-6tj2k1yn 139 34 ( ( -LRB- cord-329245-6tj2k1yn 139 35 Corse Corse NNP cord-329245-6tj2k1yn 139 36 and and CC cord-329245-6tj2k1yn 139 37 Machamer Machamer NNP cord-329245-6tj2k1yn 139 38 , , , cord-329245-6tj2k1yn 139 39 2000 2000 CD cord-329245-6tj2k1yn 139 40 ) ) -RRB- cord-329245-6tj2k1yn 139 41 . . . cord-329245-6tj2k1yn 140 1 Since since IN cord-329245-6tj2k1yn 140 2 we -PRON- PRP cord-329245-6tj2k1yn 140 3 were be VBD cord-329245-6tj2k1yn 140 4 interested interested JJ cord-329245-6tj2k1yn 140 5 in in IN cord-329245-6tj2k1yn 140 6 using use VBG cord-329245-6tj2k1yn 140 7 VLP VLP NNP cord-329245-6tj2k1yn 140 8 formation formation NN cord-329245-6tj2k1yn 140 9 to to TO cord-329245-6tj2k1yn 140 10 delineate delineate VB cord-329245-6tj2k1yn 140 11 portions portion NNS cord-329245-6tj2k1yn 140 12 of of IN cord-329245-6tj2k1yn 140 13 the the DT cord-329245-6tj2k1yn 140 14 IBV IBV NNP cord-329245-6tj2k1yn 140 15 E E NNP cord-329245-6tj2k1yn 140 16 and and CC cord-329245-6tj2k1yn 140 17 M M NNP cord-329245-6tj2k1yn 140 18 proteins protein NNS cord-329245-6tj2k1yn 140 19 that that WDT cord-329245-6tj2k1yn 140 20 are be VBP cord-329245-6tj2k1yn 140 21 important important JJ cord-329245-6tj2k1yn 140 22 in in IN cord-329245-6tj2k1yn 140 23 virus virus NN cord-329245-6tj2k1yn 140 24 assembly assembly NN cord-329245-6tj2k1yn 140 25 , , , cord-329245-6tj2k1yn 140 26 we -PRON- PRP cord-329245-6tj2k1yn 140 27 employed employ VBD cord-329245-6tj2k1yn 140 28 the the DT cord-329245-6tj2k1yn 140 29 expression expression NN cord-329245-6tj2k1yn 140 30 system system NN cord-329245-6tj2k1yn 140 31 used use VBN cord-329245-6tj2k1yn 140 32 by by IN cord-329245-6tj2k1yn 140 33 Rottier Rottier NNP cord-329245-6tj2k1yn 140 34 and and CC cord-329245-6tj2k1yn 140 35 colleagues colleague NNS cord-329245-6tj2k1yn 140 36 in in IN cord-329245-6tj2k1yn 140 37 their -PRON- PRP$ cord-329245-6tj2k1yn 140 38 initial initial JJ cord-329245-6tj2k1yn 140 39 description description NN cord-329245-6tj2k1yn 140 40 of of IN cord-329245-6tj2k1yn 140 41 MHV MHV NNP cord-329245-6tj2k1yn 140 42 VLPs vlp NNS cord-329245-6tj2k1yn 140 43 ( ( -LRB- cord-329245-6tj2k1yn 140 44 Vennema Vennema NNP cord-329245-6tj2k1yn 140 45 et et FW cord-329245-6tj2k1yn 140 46 al al NNP cord-329245-6tj2k1yn 140 47 . . NNP cord-329245-6tj2k1yn 140 48 , , , cord-329245-6tj2k1yn 140 49 1996 1996 CD cord-329245-6tj2k1yn 140 50 ) ) -RRB- cord-329245-6tj2k1yn 140 51 . . . cord-329245-6tj2k1yn 141 1 These these DT cord-329245-6tj2k1yn 141 2 authors author NNS cord-329245-6tj2k1yn 141 3 infected infect VBD cord-329245-6tj2k1yn 141 4 OST7 OST7 NNP cord-329245-6tj2k1yn 141 5 - - HYPH cord-329245-6tj2k1yn 141 6 1 1 CD cord-329245-6tj2k1yn 141 7 cells cell NNS cord-329245-6tj2k1yn 141 8 ( ( -LRB- cord-329245-6tj2k1yn 141 9 Elroy Elroy NNP cord-329245-6tj2k1yn 141 10 - - HYPH cord-329245-6tj2k1yn 141 11 Stein Stein NNP cord-329245-6tj2k1yn 141 12 and and CC cord-329245-6tj2k1yn 141 13 Moss Moss NNP cord-329245-6tj2k1yn 141 14 , , , cord-329245-6tj2k1yn 141 15 1990 1990 CD cord-329245-6tj2k1yn 141 16 ) ) -RRB- cord-329245-6tj2k1yn 141 17 , , , cord-329245-6tj2k1yn 141 18 which which WDT cord-329245-6tj2k1yn 141 19 are be VBP cord-329245-6tj2k1yn 141 20 osteosarcoma osteosarcoma NN cord-329245-6tj2k1yn 141 21 cells cell NNS cord-329245-6tj2k1yn 141 22 that that WDT cord-329245-6tj2k1yn 141 23 stably stably RB cord-329245-6tj2k1yn 141 24 express express VBP cord-329245-6tj2k1yn 141 25 phage phage NN cord-329245-6tj2k1yn 141 26 T7 T7 NNP cord-329245-6tj2k1yn 141 27 RNA RNA NNP cord-329245-6tj2k1yn 141 28 polymerase polymerase NN cord-329245-6tj2k1yn 141 29 , , , cord-329245-6tj2k1yn 141 30 with with IN cord-329245-6tj2k1yn 141 31 a a DT cord-329245-6tj2k1yn 141 32 vaccinia vaccinia NN cord-329245-6tj2k1yn 141 33 virus virus NN cord-329245-6tj2k1yn 141 34 encoding encode VBG cord-329245-6tj2k1yn 141 35 T7 t7 NN cord-329245-6tj2k1yn 141 36 polymerase polymerase NN cord-329245-6tj2k1yn 141 37 ( ( -LRB- cord-329245-6tj2k1yn 141 38 vTF7 vTF7 NNP cord-329245-6tj2k1yn 141 39 - - HYPH cord-329245-6tj2k1yn 141 40 3 3 CD cord-329245-6tj2k1yn 141 41 ) ) -RRB- cord-329245-6tj2k1yn 142 1 ( ( -LRB- cord-329245-6tj2k1yn 142 2 Fuerst Fuerst NNP cord-329245-6tj2k1yn 142 3 et et NNP cord-329245-6tj2k1yn 142 4 al al NNP cord-329245-6tj2k1yn 142 5 . . NNP cord-329245-6tj2k1yn 142 6 , , , cord-329245-6tj2k1yn 142 7 1986 1986 CD cord-329245-6tj2k1yn 142 8 ) ) -RRB- cord-329245-6tj2k1yn 142 9 to to TO cord-329245-6tj2k1yn 142 10 provide provide VB cord-329245-6tj2k1yn 142 11 higher high JJR cord-329245-6tj2k1yn 142 12 levels level NNS cord-329245-6tj2k1yn 142 13 of of IN cord-329245-6tj2k1yn 142 14 T7 T7 NNP cord-329245-6tj2k1yn 142 15 RNA RNA NNP cord-329245-6tj2k1yn 142 16 polymerase polymerase NN cord-329245-6tj2k1yn 142 17 . . . cord-329245-6tj2k1yn 143 1 They -PRON- PRP cord-329245-6tj2k1yn 143 2 then then RB cord-329245-6tj2k1yn 143 3 transfected transfecte VBD cord-329245-6tj2k1yn 143 4 the the DT cord-329245-6tj2k1yn 143 5 cells cell NNS cord-329245-6tj2k1yn 143 6 with with IN cord-329245-6tj2k1yn 143 7 plasmids plasmid NNS cord-329245-6tj2k1yn 143 8 encoding encode VBG cord-329245-6tj2k1yn 143 9 MHV MHV NNP cord-329245-6tj2k1yn 143 10 E E NNP cord-329245-6tj2k1yn 143 11 and and CC cord-329245-6tj2k1yn 143 12 M M NNP cord-329245-6tj2k1yn 143 13 behind behind IN cord-329245-6tj2k1yn 143 14 the the DT cord-329245-6tj2k1yn 143 15 T7 T7 NNP cord-329245-6tj2k1yn 143 16 promoter promoter NN cord-329245-6tj2k1yn 143 17 . . . cord-329245-6tj2k1yn 144 1 When when WRB cord-329245-6tj2k1yn 144 2 we -PRON- PRP cord-329245-6tj2k1yn 144 3 expressed express VBD cord-329245-6tj2k1yn 144 4 the the DT cord-329245-6tj2k1yn 144 5 IBV IBV NNP cord-329245-6tj2k1yn 144 6 E E NNP cord-329245-6tj2k1yn 144 7 and and CC cord-329245-6tj2k1yn 144 8 M M NNP cord-329245-6tj2k1yn 144 9 proteins protein NNS cord-329245-6tj2k1yn 144 10 this this DT cord-329245-6tj2k1yn 144 11 way way NN cord-329245-6tj2k1yn 144 12 , , , cord-329245-6tj2k1yn 144 13 we -PRON- PRP cord-329245-6tj2k1yn 144 14 found find VBD cord-329245-6tj2k1yn 144 15 that that IN cord-329245-6tj2k1yn 144 16 VLPs vlp NNS cord-329245-6tj2k1yn 144 17 were be VBD cord-329245-6tj2k1yn 144 18 formed form VBN cord-329245-6tj2k1yn 144 19 efficiently efficiently RB cord-329245-6tj2k1yn 144 20 ( ( -LRB- cord-329245-6tj2k1yn 144 21 Fig fig NN cord-329245-6tj2k1yn 144 22 . . . cord-329245-6tj2k1yn 144 23 _SP cord-329245-6tj2k1yn 145 1 6 6 CD cord-329245-6tj2k1yn 145 2 , , , cord-329245-6tj2k1yn 145 3 lanes lane VBZ cord-329245-6tj2k1yn 145 4 3 3 CD cord-329245-6tj2k1yn 145 5 and and CC cord-329245-6tj2k1yn 145 6 14 14 CD cord-329245-6tj2k1yn 145 7 ) ) -RRB- cord-329245-6tj2k1yn 145 8 . . . cord-329245-6tj2k1yn 146 1 The the DT cord-329245-6tj2k1yn 146 2 supernatant supernatant JJ cord-329245-6tj2k1yn 146 3 side side NN cord-329245-6tj2k1yn 146 4 of of IN cord-329245-6tj2k1yn 146 5 the the DT cord-329245-6tj2k1yn 146 6 gel gel NN cord-329245-6tj2k1yn 146 7 ( ( -LRB- cord-329245-6tj2k1yn 146 8 lanes lane NNS cord-329245-6tj2k1yn 146 9 12 12 CD cord-329245-6tj2k1yn 146 10 - - SYM cord-329245-6tj2k1yn 146 11 22 22 CD cord-329245-6tj2k1yn 146 12 ) ) -RRB- cord-329245-6tj2k1yn 146 13 shown show VBN cord-329245-6tj2k1yn 146 14 in in IN cord-329245-6tj2k1yn 146 15 this this DT cord-329245-6tj2k1yn 146 16 figure figure NN cord-329245-6tj2k1yn 146 17 was be VBD cord-329245-6tj2k1yn 146 18 exposed expose VBN cord-329245-6tj2k1yn 146 19 20 20 CD cord-329245-6tj2k1yn 146 20 times time NNS cord-329245-6tj2k1yn 146 21 longer long JJR cord-329245-6tj2k1yn 146 22 than than IN cord-329245-6tj2k1yn 146 23 the the DT cord-329245-6tj2k1yn 146 24 cell cell NN cord-329245-6tj2k1yn 146 25 side side NN cord-329245-6tj2k1yn 146 26 to to TO cord-329245-6tj2k1yn 146 27 allow allow VB cord-329245-6tj2k1yn 146 28 the the DT cord-329245-6tj2k1yn 146 29 visualization visualization NN cord-329245-6tj2k1yn 146 30 of of IN cord-329245-6tj2k1yn 146 31 E e NN cord-329245-6tj2k1yn 146 32 and and CC cord-329245-6tj2k1yn 146 33 E e NN cord-329245-6tj2k1yn 146 34 transmembrane transmembrane NN cord-329245-6tj2k1yn 146 35 mutant mutant JJ cord-329245-6tj2k1yn 146 36 proteins protein NNS cord-329245-6tj2k1yn 147 1 ( ( -LRB- cord-329245-6tj2k1yn 147 2 lanes lane NNS cord-329245-6tj2k1yn 147 3 1 1 CD cord-329245-6tj2k1yn 147 4 - - SYM cord-329245-6tj2k1yn 147 5 11 11 CD cord-329245-6tj2k1yn 147 6 ) ) -RRB- cord-329245-6tj2k1yn 147 7 . . . cord-329245-6tj2k1yn 148 1 We -PRON- PRP cord-329245-6tj2k1yn 148 2 have have VBP cord-329245-6tj2k1yn 148 3 consistently consistently RB cord-329245-6tj2k1yn 148 4 observed observe VBN cord-329245-6tj2k1yn 148 5 at at IN cord-329245-6tj2k1yn 148 6 least least RBS cord-329245-6tj2k1yn 148 7 10 10 CD cord-329245-6tj2k1yn 148 8 % % NN cord-329245-6tj2k1yn 148 9 , , , cord-329245-6tj2k1yn 148 10 and and CC cord-329245-6tj2k1yn 148 11 frequently frequently RB cord-329245-6tj2k1yn 148 12 more more JJR cord-329245-6tj2k1yn 148 13 , , , cord-329245-6tj2k1yn 148 14 of of IN cord-329245-6tj2k1yn 148 15 the the DT cord-329245-6tj2k1yn 148 16 cellular cellular JJ cord-329245-6tj2k1yn 148 17 M M NNP cord-329245-6tj2k1yn 148 18 protein protein NN cord-329245-6tj2k1yn 148 19 being be VBG cord-329245-6tj2k1yn 148 20 released release VBN cord-329245-6tj2k1yn 148 21 into into IN cord-329245-6tj2k1yn 148 22 the the DT cord-329245-6tj2k1yn 148 23 supernatant supernatant NN cord-329245-6tj2k1yn 148 24 during during IN cord-329245-6tj2k1yn 148 25 the the DT cord-329245-6tj2k1yn 148 26 3 3 CD cord-329245-6tj2k1yn 148 27 h h NN cord-329245-6tj2k1yn 148 28 chase chase NN cord-329245-6tj2k1yn 148 29 ( ( -LRB- cord-329245-6tj2k1yn 148 30 data datum NNS cord-329245-6tj2k1yn 148 31 not not RB cord-329245-6tj2k1yn 148 32 shown show VBN cord-329245-6tj2k1yn 148 33 ) ) -RRB- cord-329245-6tj2k1yn 148 34 . . . cord-329245-6tj2k1yn 149 1 This this DT cord-329245-6tj2k1yn 149 2 level level NN cord-329245-6tj2k1yn 149 3 of of IN cord-329245-6tj2k1yn 149 4 VLP VLP NNP cord-329245-6tj2k1yn 149 5 release release NN cord-329245-6tj2k1yn 149 6 was be VBD cord-329245-6tj2k1yn 149 7 dependent dependent JJ cord-329245-6tj2k1yn 149 8 on on IN cord-329245-6tj2k1yn 149 9 E e NN cord-329245-6tj2k1yn 149 10 protein protein NN cord-329245-6tj2k1yn 149 11 , , , cord-329245-6tj2k1yn 149 12 since since IN cord-329245-6tj2k1yn 149 13 less less JJR cord-329245-6tj2k1yn 149 14 than than IN cord-329245-6tj2k1yn 149 15 1 1 CD cord-329245-6tj2k1yn 149 16 % % NN cord-329245-6tj2k1yn 149 17 of of IN cord-329245-6tj2k1yn 149 18 the the DT cord-329245-6tj2k1yn 149 19 cellular cellular JJ cord-329245-6tj2k1yn 149 20 M M NNP cord-329245-6tj2k1yn 149 21 protein protein NN cord-329245-6tj2k1yn 149 22 was be VBD cord-329245-6tj2k1yn 149 23 found find VBN cord-329245-6tj2k1yn 149 24 in in IN cord-329245-6tj2k1yn 149 25 the the DT cord-329245-6tj2k1yn 149 26 supernatant supernatant NN cord-329245-6tj2k1yn 149 27 in in IN cord-329245-6tj2k1yn 149 28 the the DT cord-329245-6tj2k1yn 149 29 absence absence NN cord-329245-6tj2k1yn 149 30 of of IN cord-329245-6tj2k1yn 149 31 E. E. NNP cord-329245-6tj2k1yn 149 32 E E NNP cord-329245-6tj2k1yn 149 33 protein protein NN cord-329245-6tj2k1yn 149 34 was be VBD cord-329245-6tj2k1yn 149 35 not not RB cord-329245-6tj2k1yn 149 36 visualized visualize VBN cord-329245-6tj2k1yn 149 37 in in IN cord-329245-6tj2k1yn 149 38 the the DT cord-329245-6tj2k1yn 149 39 supernatants supernatant NNS cord-329245-6tj2k1yn 149 40 when when WRB cord-329245-6tj2k1yn 149 41 expressed express VBN cord-329245-6tj2k1yn 149 42 alone alone RB cord-329245-6tj2k1yn 150 1 ( ( -LRB- cord-329245-6tj2k1yn 150 2 Fig Fig NNP cord-329245-6tj2k1yn 150 3 . . NNP cord-329245-6tj2k1yn 150 4 6 6 CD cord-329245-6tj2k1yn 150 5 , , , cord-329245-6tj2k1yn 150 6 lane lane NN cord-329245-6tj2k1yn 150 7 12 12 CD cord-329245-6tj2k1yn 150 8 ) ) -RRB- cord-329245-6tj2k1yn 150 9 . . . cord-329245-6tj2k1yn 151 1 We -PRON- PRP cord-329245-6tj2k1yn 151 2 believe believe VBP cord-329245-6tj2k1yn 151 3 that that IN cord-329245-6tj2k1yn 151 4 a a DT cord-329245-6tj2k1yn 151 5 small small JJ cord-329245-6tj2k1yn 151 6 amount amount NN cord-329245-6tj2k1yn 151 7 of of IN cord-329245-6tj2k1yn 151 8 E e NN cord-329245-6tj2k1yn 151 9 is be VBZ cord-329245-6tj2k1yn 151 10 released release VBN cord-329245-6tj2k1yn 151 11 into into IN cord-329245-6tj2k1yn 151 12 the the DT cord-329245-6tj2k1yn 151 13 supernatant supernatant NN cord-329245-6tj2k1yn 151 14 when when WRB cord-329245-6tj2k1yn 151 15 it -PRON- PRP cord-329245-6tj2k1yn 151 16 is be VBZ cord-329245-6tj2k1yn 151 17 expressed express VBN cord-329245-6tj2k1yn 151 18 alone alone JJ cord-329245-6tj2k1yn 151 19 , , , cord-329245-6tj2k1yn 151 20 and and CC cord-329245-6tj2k1yn 151 21 that that IN cord-329245-6tj2k1yn 151 22 it -PRON- PRP cord-329245-6tj2k1yn 151 23 is be VBZ cord-329245-6tj2k1yn 151 24 simply simply RB cord-329245-6tj2k1yn 151 25 not not RB cord-329245-6tj2k1yn 151 26 visualized visualize VBN cord-329245-6tj2k1yn 151 27 unless unless IN cord-329245-6tj2k1yn 151 28 a a DT cord-329245-6tj2k1yn 151 29 large large JJ cord-329245-6tj2k1yn 151 30 amount amount NN cord-329245-6tj2k1yn 151 31 of of IN cord-329245-6tj2k1yn 151 32 supernatant supernatant NN cord-329245-6tj2k1yn 151 33 is be VBZ cord-329245-6tj2k1yn 151 34 loaded load VBN cord-329245-6tj2k1yn 151 35 on on IN cord-329245-6tj2k1yn 151 36 the the DT cord-329245-6tj2k1yn 151 37 gel gel NN cord-329245-6tj2k1yn 151 38 , , , cord-329245-6tj2k1yn 151 39 as as IN cord-329245-6tj2k1yn 151 40 in in IN cord-329245-6tj2k1yn 151 41 our -PRON- PRP$ cord-329245-6tj2k1yn 151 42 previous previous JJ cord-329245-6tj2k1yn 151 43 experiments experiment NNS cord-329245-6tj2k1yn 151 44 ( ( -LRB- cord-329245-6tj2k1yn 151 45 Corse Corse NNP cord-329245-6tj2k1yn 151 46 and and CC cord-329245-6tj2k1yn 151 47 Machamer Machamer NNP cord-329245-6tj2k1yn 151 48 , , , cord-329245-6tj2k1yn 151 49 2000 2000 CD cord-329245-6tj2k1yn 151 50 ) ) -RRB- cord-329245-6tj2k1yn 151 51 . . . cord-329245-6tj2k1yn 152 1 Our -PRON- PRP$ cord-329245-6tj2k1yn 152 2 results result NNS cord-329245-6tj2k1yn 152 3 differ differ VBP cord-329245-6tj2k1yn 152 4 from from IN cord-329245-6tj2k1yn 152 5 the the DT cord-329245-6tj2k1yn 152 6 data datum NNS cord-329245-6tj2k1yn 152 7 of of IN cord-329245-6tj2k1yn 152 8 Makino Makino NNP cord-329245-6tj2k1yn 152 9 and and CC cord-329245-6tj2k1yn 152 10 colleagues colleague NNS cord-329245-6tj2k1yn 152 11 , , , cord-329245-6tj2k1yn 152 12 who who WP cord-329245-6tj2k1yn 152 13 observed observe VBD cord-329245-6tj2k1yn 152 14 a a DT cord-329245-6tj2k1yn 152 15 substantial substantial JJ cord-329245-6tj2k1yn 152 16 amount amount NN cord-329245-6tj2k1yn 152 17 of of IN cord-329245-6tj2k1yn 152 18 MHV MHV NNP cord-329245-6tj2k1yn 152 19 E E NNP cord-329245-6tj2k1yn 152 20 being be VBG cord-329245-6tj2k1yn 152 21 released release VBN cord-329245-6tj2k1yn 152 22 into into IN cord-329245-6tj2k1yn 152 23 the the DT cord-329245-6tj2k1yn 152 24 supernatant supernatant NN cord-329245-6tj2k1yn 152 25 when when WRB cord-329245-6tj2k1yn 152 26 expressed express VBN cord-329245-6tj2k1yn 152 27 by by IN cord-329245-6tj2k1yn 152 28 itself -PRON- PRP cord-329245-6tj2k1yn 152 29 ( ( -LRB- cord-329245-6tj2k1yn 152 30 Maeda Maeda NNP cord-329245-6tj2k1yn 152 31 et et NNP cord-329245-6tj2k1yn 152 32 al al NNP cord-329245-6tj2k1yn 152 33 . . NNP cord-329245-6tj2k1yn 152 34 , , , cord-329245-6tj2k1yn 152 35 1999 1999 CD cord-329245-6tj2k1yn 152 36 ) ) -RRB- cord-329245-6tj2k1yn 152 37 ; ; : cord-329245-6tj2k1yn 152 38 this this DT cord-329245-6tj2k1yn 152 39 discrepancy discrepancy NN cord-329245-6tj2k1yn 152 40 could could MD cord-329245-6tj2k1yn 152 41 be be VB cord-329245-6tj2k1yn 152 42 explained explain VBN cord-329245-6tj2k1yn 152 43 by by IN cord-329245-6tj2k1yn 152 44 the the DT cord-329245-6tj2k1yn 152 45 different different JJ cord-329245-6tj2k1yn 152 46 expression expression NN cord-329245-6tj2k1yn 152 47 system system NN cord-329245-6tj2k1yn 152 48 used use VBN cord-329245-6tj2k1yn 152 49 to to TO cord-329245-6tj2k1yn 152 50 produce produce VB cord-329245-6tj2k1yn 152 51 the the DT cord-329245-6tj2k1yn 152 52 E e NN cord-329245-6tj2k1yn 152 53 protein protein NN cord-329245-6tj2k1yn 152 54 in in IN cord-329245-6tj2k1yn 152 55 their -PRON- PRP$ cord-329245-6tj2k1yn 152 56 study study NN cord-329245-6tj2k1yn 152 57 . . . cord-329245-6tj2k1yn 153 1 We -PRON- PRP cord-329245-6tj2k1yn 153 2 favor favor VBP cord-329245-6tj2k1yn 153 3 the the DT cord-329245-6tj2k1yn 153 4 hypothesis hypothesis NN cord-329245-6tj2k1yn 153 5 that that IN cord-329245-6tj2k1yn 153 6 VLP VLP NNP cord-329245-6tj2k1yn 153 7 formation formation NN cord-329245-6tj2k1yn 153 8 involves involve VBZ cord-329245-6tj2k1yn 153 9 a a DT cord-329245-6tj2k1yn 153 10 lattice lattice NN cord-329245-6tj2k1yn 153 11 of of IN cord-329245-6tj2k1yn 153 12 M M NNP cord-329245-6tj2k1yn 153 13 protein protein NN cord-329245-6tj2k1yn 153 14 within within IN cord-329245-6tj2k1yn 153 15 the the DT cord-329245-6tj2k1yn 153 16 membrane membrane NN cord-329245-6tj2k1yn 153 17 with with IN cord-329245-6tj2k1yn 153 18 curvature curvature NN cord-329245-6tj2k1yn 153 19 induced induce VBN cord-329245-6tj2k1yn 153 20 by by IN cord-329245-6tj2k1yn 153 21 small small JJ cord-329245-6tj2k1yn 153 22 amounts amount NNS cord-329245-6tj2k1yn 153 23 of of IN cord-329245-6tj2k1yn 153 24 the the DT cord-329245-6tj2k1yn 153 25 E e NN cord-329245-6tj2k1yn 153 26 protein protein NN cord-329245-6tj2k1yn 153 27 ( ( -LRB- cord-329245-6tj2k1yn 153 28 see see VB cord-329245-6tj2k1yn 153 29 Discussion Discussion NNP cord-329245-6tj2k1yn 153 30 ) ) -RRB- cord-329245-6tj2k1yn 153 31 . . . cord-329245-6tj2k1yn 154 1 This this DT cord-329245-6tj2k1yn 154 2 would would MD cord-329245-6tj2k1yn 154 3 account account VB cord-329245-6tj2k1yn 154 4 for for IN cord-329245-6tj2k1yn 154 5 the the DT cord-329245-6tj2k1yn 154 6 small small JJ cord-329245-6tj2k1yn 154 7 amount amount NN cord-329245-6tj2k1yn 154 8 of of IN cord-329245-6tj2k1yn 154 9 IBV IBV NNP cord-329245-6tj2k1yn 154 10 E e NN cord-329245-6tj2k1yn 154 11 protein protein NN cord-329245-6tj2k1yn 154 12 relative relative NN cord-329245-6tj2k1yn 154 13 to to IN cord-329245-6tj2k1yn 154 14 IBV IBV NNP cord-329245-6tj2k1yn 154 15 M M NNP cord-329245-6tj2k1yn 154 16 protein protein NN cord-329245-6tj2k1yn 154 17 that that WDT cord-329245-6tj2k1yn 154 18 is be VBZ cord-329245-6tj2k1yn 154 19 found find VBN cord-329245-6tj2k1yn 154 20 in in IN cord-329245-6tj2k1yn 154 21 VLPs vlp NNS cord-329245-6tj2k1yn 154 22 . . . cord-329245-6tj2k1yn 155 1 We -PRON- PRP cord-329245-6tj2k1yn 155 2 found find VBD cord-329245-6tj2k1yn 155 3 that that IN cord-329245-6tj2k1yn 155 4 the the DT cord-329245-6tj2k1yn 155 5 partial partial JJ cord-329245-6tj2k1yn 155 6 or or CC cord-329245-6tj2k1yn 155 7 complete complete JJ cord-329245-6tj2k1yn 155 8 replacement replacement NN cord-329245-6tj2k1yn 155 9 of of IN cord-329245-6tj2k1yn 155 10 the the DT cord-329245-6tj2k1yn 155 11 IBV IBV NNP cord-329245-6tj2k1yn 155 12 E e NN cord-329245-6tj2k1yn 155 13 transmembrane transmembrane NN cord-329245-6tj2k1yn 155 14 domain domain NN cord-329245-6tj2k1yn 155 15 ( ( -LRB- cord-329245-6tj2k1yn 155 16 mutants mutant NNS cord-329245-6tj2k1yn 155 17 EG1 EG1 NNP cord-329245-6tj2k1yn 155 18 , , , cord-329245-6tj2k1yn 155 19 EG2 EG2 NNP cord-329245-6tj2k1yn 155 20 , , , cord-329245-6tj2k1yn 155 21 and and CC cord-329245-6tj2k1yn 155 22 EG3 EG3 NNP cord-329245-6tj2k1yn 155 23 ) ) -RRB- cord-329245-6tj2k1yn 155 24 had have VBD cord-329245-6tj2k1yn 155 25 no no DT cord-329245-6tj2k1yn 155 26 effect effect NN cord-329245-6tj2k1yn 155 27 on on IN cord-329245-6tj2k1yn 155 28 E E NNP cord-329245-6tj2k1yn 155 29 's 's POS cord-329245-6tj2k1yn 155 30 ability ability NN cord-329245-6tj2k1yn 155 31 to to TO cord-329245-6tj2k1yn 155 32 induce induce VB cord-329245-6tj2k1yn 155 33 VLPs vlp NNS cord-329245-6tj2k1yn 155 34 as as IN cord-329245-6tj2k1yn 155 35 measured measure VBN cord-329245-6tj2k1yn 155 36 by by IN cord-329245-6tj2k1yn 155 37 the the DT cord-329245-6tj2k1yn 155 38 amount amount NN cord-329245-6tj2k1yn 155 39 of of IN cord-329245-6tj2k1yn 155 40 IBV IBV NNP cord-329245-6tj2k1yn 155 41 M M NNP cord-329245-6tj2k1yn 155 42 protein protein NN cord-329245-6tj2k1yn 155 43 released release VBD cord-329245-6tj2k1yn 155 44 into into IN cord-329245-6tj2k1yn 155 45 the the DT cord-329245-6tj2k1yn 155 46 supernatant supernatant NN cord-329245-6tj2k1yn 155 47 ( ( -LRB- cord-329245-6tj2k1yn 155 48 Fig Fig NNP cord-329245-6tj2k1yn 155 49 . . NNP cord-329245-6tj2k1yn 155 50 6 6 CD cord-329245-6tj2k1yn 155 51 , , , cord-329245-6tj2k1yn 155 52 compare compare VB cord-329245-6tj2k1yn 155 53 the the DT cord-329245-6tj2k1yn 155 54 M M NNP cord-329245-6tj2k1yn 155 55 protein protein NN cord-329245-6tj2k1yn 155 56 bands band NNS cord-329245-6tj2k1yn 155 57 in in IN cord-329245-6tj2k1yn 155 58 lanes lane NNS cord-329245-6tj2k1yn 155 59 14 14 CD cord-329245-6tj2k1yn 155 60 , , , cord-329245-6tj2k1yn 155 61 16 16 CD cord-329245-6tj2k1yn 155 62 , , , cord-329245-6tj2k1yn 155 63 18 18 CD cord-329245-6tj2k1yn 155 64 , , , cord-329245-6tj2k1yn 155 65 and and CC cord-329245-6tj2k1yn 155 66 20 20 CD cord-329245-6tj2k1yn 155 67 ) ) -RRB- cord-329245-6tj2k1yn 155 68 . . . cord-329245-6tj2k1yn 156 1 We -PRON- PRP cord-329245-6tj2k1yn 156 2 did do VBD cord-329245-6tj2k1yn 156 3 notice notice VB cord-329245-6tj2k1yn 156 4 that that IN cord-329245-6tj2k1yn 156 5 more more RBR cord-329245-6tj2k1yn 156 6 EG2 eg2 JJ cord-329245-6tj2k1yn 156 7 and and CC cord-329245-6tj2k1yn 156 8 EG3 eg3 JJ cord-329245-6tj2k1yn 156 9 proteins protein NNS cord-329245-6tj2k1yn 156 10 were be VBD cord-329245-6tj2k1yn 156 11 incorporated incorporate VBN cord-329245-6tj2k1yn 156 12 into into IN cord-329245-6tj2k1yn 156 13 VLPs vlp NNS cord-329245-6tj2k1yn 156 14 compared compare VBN cord-329245-6tj2k1yn 156 15 to to IN cord-329245-6tj2k1yn 156 16 wildtype wildtype NN cord-329245-6tj2k1yn 156 17 E e NN cord-329245-6tj2k1yn 156 18 and and CC cord-329245-6tj2k1yn 156 19 EG1 eg1 JJ cord-329245-6tj2k1yn 156 20 proteins protein NNS cord-329245-6tj2k1yn 156 21 ( ( -LRB- cord-329245-6tj2k1yn 156 22 Fig Fig NNP cord-329245-6tj2k1yn 156 23 . . NNP cord-329245-6tj2k1yn 156 24 6 6 CD cord-329245-6tj2k1yn 156 25 , , , cord-329245-6tj2k1yn 156 26 compare compare VB cord-329245-6tj2k1yn 156 27 the the DT cord-329245-6tj2k1yn 156 28 E e NN cord-329245-6tj2k1yn 156 29 protein protein NN cord-329245-6tj2k1yn 156 30 bands band NNS cord-329245-6tj2k1yn 156 31 in in IN cord-329245-6tj2k1yn 156 32 lanes lane NNS cord-329245-6tj2k1yn 156 33 18 18 CD cord-329245-6tj2k1yn 156 34 and and CC cord-329245-6tj2k1yn 156 35 20 20 CD cord-329245-6tj2k1yn 156 36 with with IN cord-329245-6tj2k1yn 156 37 those those DT cord-329245-6tj2k1yn 156 38 in in IN cord-329245-6tj2k1yn 156 39 lanes lane NNS cord-329245-6tj2k1yn 156 40 14 14 CD cord-329245-6tj2k1yn 156 41 and and CC cord-329245-6tj2k1yn 156 42 16 16 CD cord-329245-6tj2k1yn 156 43 ) ) -RRB- cord-329245-6tj2k1yn 156 44 . . . cord-329245-6tj2k1yn 157 1 It -PRON- PRP cord-329245-6tj2k1yn 157 2 is be VBZ cord-329245-6tj2k1yn 157 3 possible possible JJ cord-329245-6tj2k1yn 157 4 that that IN cord-329245-6tj2k1yn 157 5 conformational conformational JJ cord-329245-6tj2k1yn 157 6 changes change NNS cord-329245-6tj2k1yn 157 7 induced induce VBN cord-329245-6tj2k1yn 157 8 by by IN cord-329245-6tj2k1yn 157 9 replacement replacement NN cord-329245-6tj2k1yn 157 10 of of IN cord-329245-6tj2k1yn 157 11 the the DT cord-329245-6tj2k1yn 157 12 transmembrane transmembrane NN cord-329245-6tj2k1yn 157 13 domain domain NN cord-329245-6tj2k1yn 157 14 facilitates facilitate VBZ cord-329245-6tj2k1yn 157 15 increased increase VBN cord-329245-6tj2k1yn 157 16 incorporation incorporation NN cord-329245-6tj2k1yn 157 17 of of IN cord-329245-6tj2k1yn 157 18 these these DT cord-329245-6tj2k1yn 157 19 mutants mutant NNS cord-329245-6tj2k1yn 157 20 into into IN cord-329245-6tj2k1yn 157 21 VLPs vlp NNS cord-329245-6tj2k1yn 157 22 . . . cord-329245-6tj2k1yn 158 1 The the DT cord-329245-6tj2k1yn 158 2 EG2 EG2 NNP cord-329245-6tj2k1yn 158 3 and and CC cord-329245-6tj2k1yn 158 4 EG3 EG3 NNP cord-329245-6tj2k1yn 158 5 mutants mutant NNS cord-329245-6tj2k1yn 158 6 did do VBD cord-329245-6tj2k1yn 158 7 not not RB cord-329245-6tj2k1yn 158 8 induce induce VB cord-329245-6tj2k1yn 158 9 VLPs vlp NNS cord-329245-6tj2k1yn 158 10 more more RBR cord-329245-6tj2k1yn 158 11 efficiently efficiently RB cord-329245-6tj2k1yn 158 12 than than IN cord-329245-6tj2k1yn 158 13 wild wild JJ cord-329245-6tj2k1yn 158 14 - - HYPH cord-329245-6tj2k1yn 158 15 type type NN cord-329245-6tj2k1yn 158 16 E e NN cord-329245-6tj2k1yn 158 17 , , , cord-329245-6tj2k1yn 158 18 because because IN cord-329245-6tj2k1yn 158 19 an an DT cord-329245-6tj2k1yn 158 20 increased increase VBN cord-329245-6tj2k1yn 158 21 amount amount NN cord-329245-6tj2k1yn 158 22 of of IN cord-329245-6tj2k1yn 158 23 M M NNP cord-329245-6tj2k1yn 158 24 protein protein NN cord-329245-6tj2k1yn 158 25 was be VBD cord-329245-6tj2k1yn 158 26 not not RB cord-329245-6tj2k1yn 158 27 released release VBN cord-329245-6tj2k1yn 158 28 into into IN cord-329245-6tj2k1yn 158 29 the the DT cord-329245-6tj2k1yn 158 30 supernatant supernatant NN cord-329245-6tj2k1yn 158 31 . . . cord-329245-6tj2k1yn 159 1 We -PRON- PRP cord-329245-6tj2k1yn 159 2 examined examine VBD cord-329245-6tj2k1yn 159 3 the the DT cord-329245-6tj2k1yn 159 4 ability ability NN cord-329245-6tj2k1yn 159 5 of of IN cord-329245-6tj2k1yn 159 6 the the DT cord-329245-6tj2k1yn 159 7 E e NN cord-329245-6tj2k1yn 159 8 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 159 9 tail tail NN cord-329245-6tj2k1yn 159 10 to to TO cord-329245-6tj2k1yn 159 11 induce induce VB cord-329245-6tj2k1yn 159 12 VLPs vlp NNS cord-329245-6tj2k1yn 159 13 by by IN cord-329245-6tj2k1yn 159 14 using use VBG cord-329245-6tj2k1yn 159 15 the the DT cord-329245-6tj2k1yn 159 16 chimeric chimeric JJ cord-329245-6tj2k1yn 159 17 protein protein NN cord-329245-6tj2k1yn 159 18 GEt GEt NNS cord-329245-6tj2k1yn 159 19 . . . cord-329245-6tj2k1yn 160 1 When when WRB cord-329245-6tj2k1yn 160 2 expressed express VBN cord-329245-6tj2k1yn 160 3 by by IN cord-329245-6tj2k1yn 160 4 itself -PRON- PRP cord-329245-6tj2k1yn 160 5 , , , cord-329245-6tj2k1yn 160 6 we -PRON- PRP cord-329245-6tj2k1yn 160 7 observed observe VBD cord-329245-6tj2k1yn 160 8 that that IN cord-329245-6tj2k1yn 160 9 a a DT cord-329245-6tj2k1yn 160 10 negligible negligible JJ cord-329245-6tj2k1yn 160 11 amount amount NN cord-329245-6tj2k1yn 160 12 of of IN cord-329245-6tj2k1yn 160 13 this this DT cord-329245-6tj2k1yn 160 14 protein protein NN cord-329245-6tj2k1yn 160 15 was be VBD cord-329245-6tj2k1yn 160 16 released release VBN cord-329245-6tj2k1yn 160 17 into into IN cord-329245-6tj2k1yn 160 18 the the DT cord-329245-6tj2k1yn 160 19 supernatant supernatant NN cord-329245-6tj2k1yn 160 20 ( ( -LRB- cord-329245-6tj2k1yn 160 21 Fig Fig NNP cord-329245-6tj2k1yn 160 22 . . NNP cord-329245-6tj2k1yn 160 23 6 6 CD cord-329245-6tj2k1yn 160 24 , , , cord-329245-6tj2k1yn 160 25 lane lane NN cord-329245-6tj2k1yn 160 26 21 21 CD cord-329245-6tj2k1yn 160 27 ; ; : cord-329245-6tj2k1yn 160 28 recall recall VBP cord-329245-6tj2k1yn 160 29 that that IN cord-329245-6tj2k1yn 160 30 the the DT cord-329245-6tj2k1yn 160 31 supernatant supernatant JJ cord-329245-6tj2k1yn 160 32 side side NN cord-329245-6tj2k1yn 160 33 of of IN cord-329245-6tj2k1yn 160 34 the the DT cord-329245-6tj2k1yn 160 35 gel gel NN cord-329245-6tj2k1yn 160 36 was be VBD cord-329245-6tj2k1yn 160 37 exposed expose VBN cord-329245-6tj2k1yn 160 38 20 20 CD cord-329245-6tj2k1yn 160 39 times time NNS cord-329245-6tj2k1yn 160 40 longer long JJR cord-329245-6tj2k1yn 160 41 than than IN cord-329245-6tj2k1yn 160 42 the the DT cord-329245-6tj2k1yn 160 43 cell cell NN cord-329245-6tj2k1yn 160 44 side side NN cord-329245-6tj2k1yn 160 45 ) ) -RRB- cord-329245-6tj2k1yn 160 46 and and CC cord-329245-6tj2k1yn 160 47 that that DT cord-329245-6tj2k1yn 160 48 expression expression NN cord-329245-6tj2k1yn 160 49 of of IN cord-329245-6tj2k1yn 160 50 GEt GEt NNS cord-329245-6tj2k1yn 160 51 with with IN cord-329245-6tj2k1yn 160 52 IBV IBV NNP cord-329245-6tj2k1yn 160 53 M M NNP cord-329245-6tj2k1yn 160 54 did do VBD cord-329245-6tj2k1yn 160 55 not not RB cord-329245-6tj2k1yn 160 56 increase increase VB cord-329245-6tj2k1yn 160 57 the the DT cord-329245-6tj2k1yn 160 58 amount amount NN cord-329245-6tj2k1yn 160 59 of of IN cord-329245-6tj2k1yn 160 60 M M NNP cord-329245-6tj2k1yn 160 61 that that WDT cord-329245-6tj2k1yn 160 62 was be VBD cord-329245-6tj2k1yn 160 63 released release VBN cord-329245-6tj2k1yn 160 64 when when WRB cord-329245-6tj2k1yn 160 65 M M NNP cord-329245-6tj2k1yn 160 66 was be VBD cord-329245-6tj2k1yn 160 67 expressed express VBN cord-329245-6tj2k1yn 160 68 alone alone JJ cord-329245-6tj2k1yn 160 69 ( ( -LRB- cord-329245-6tj2k1yn 160 70 Fig Fig NNP cord-329245-6tj2k1yn 160 71 . . NNP cord-329245-6tj2k1yn 160 72 6 6 CD cord-329245-6tj2k1yn 160 73 , , , cord-329245-6tj2k1yn 160 74 lanes lane VBZ cord-329245-6tj2k1yn 160 75 22 22 CD cord-329245-6tj2k1yn 160 76 and and CC cord-329245-6tj2k1yn 160 77 _SP cord-329245-6tj2k1yn 160 78 13 13 CD cord-329245-6tj2k1yn 160 79 ) ) -RRB- cord-329245-6tj2k1yn 160 80 . . . cord-329245-6tj2k1yn 161 1 We -PRON- PRP cord-329245-6tj2k1yn 161 2 conclude conclude VBP cord-329245-6tj2k1yn 161 3 that that IN cord-329245-6tj2k1yn 161 4 the the DT cord-329245-6tj2k1yn 161 5 GEt GEt NNS cord-329245-6tj2k1yn 161 6 chimera chimera NN cord-329245-6tj2k1yn 161 7 does do VBZ cord-329245-6tj2k1yn 161 8 not not RB cord-329245-6tj2k1yn 161 9 induce induce VB cord-329245-6tj2k1yn 161 10 VLPs vlp NNS cord-329245-6tj2k1yn 161 11 . . . cord-329245-6tj2k1yn 162 1 Since since IN cord-329245-6tj2k1yn 162 2 both both CC cord-329245-6tj2k1yn 162 3 the the DT cord-329245-6tj2k1yn 162 4 EG3 EG3 NNP cord-329245-6tj2k1yn 162 5 and and CC cord-329245-6tj2k1yn 162 6 the the DT cord-329245-6tj2k1yn 162 7 GEt GEt NNS cord-329245-6tj2k1yn 163 1 chimeras chimera NNS cord-329245-6tj2k1yn 163 2 contain contain VBP cord-329245-6tj2k1yn 163 3 the the DT cord-329245-6tj2k1yn 163 4 transmembrane transmembrane NN cord-329245-6tj2k1yn 163 5 domain domain NN cord-329245-6tj2k1yn 163 6 of of IN cord-329245-6tj2k1yn 163 7 VSV VSV NNP cord-329245-6tj2k1yn 163 8 G G NNP cord-329245-6tj2k1yn 163 9 and and CC cord-329245-6tj2k1yn 163 10 the the DT cord-329245-6tj2k1yn 163 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 163 12 tail tail NN cord-329245-6tj2k1yn 163 13 of of IN cord-329245-6tj2k1yn 163 14 IBV IBV NNP cord-329245-6tj2k1yn 163 15 E E NNP cord-329245-6tj2k1yn 163 16 , , , cord-329245-6tj2k1yn 163 17 it -PRON- PRP cord-329245-6tj2k1yn 163 18 seems seem VBZ cord-329245-6tj2k1yn 163 19 likely likely JJ cord-329245-6tj2k1yn 163 20 that that IN cord-329245-6tj2k1yn 163 21 the the DT cord-329245-6tj2k1yn 163 22 VSV VSV NNP cord-329245-6tj2k1yn 163 23 G G NNP cord-329245-6tj2k1yn 163 24 lumenal lumenal JJ cord-329245-6tj2k1yn 163 25 domain domain NN cord-329245-6tj2k1yn 163 26 is be VBZ cord-329245-6tj2k1yn 163 27 incompatible incompatible JJ cord-329245-6tj2k1yn 163 28 with with IN cord-329245-6tj2k1yn 163 29 VLP VLP NNP cord-329245-6tj2k1yn 163 30 formation formation NN cord-329245-6tj2k1yn 163 31 , , , cord-329245-6tj2k1yn 163 32 either either CC cord-329245-6tj2k1yn 163 33 because because IN cord-329245-6tj2k1yn 163 34 it -PRON- PRP cord-329245-6tj2k1yn 163 35 is be VBZ cord-329245-6tj2k1yn 163 36 too too RB cord-329245-6tj2k1yn 163 37 large large JJ cord-329245-6tj2k1yn 163 38 or or CC cord-329245-6tj2k1yn 163 39 because because IN cord-329245-6tj2k1yn 163 40 it -PRON- PRP cord-329245-6tj2k1yn 163 41 can can MD cord-329245-6tj2k1yn 163 42 not not RB cord-329245-6tj2k1yn 163 43 be be VB cord-329245-6tj2k1yn 163 44 incorporated incorporate VBN cord-329245-6tj2k1yn 163 45 into into IN cord-329245-6tj2k1yn 163 46 the the DT cord-329245-6tj2k1yn 163 47 M M NNP cord-329245-6tj2k1yn 163 48 protein protein NN cord-329245-6tj2k1yn 163 49 lattice lattice NN cord-329245-6tj2k1yn 163 50 for for IN cord-329245-6tj2k1yn 163 51 some some DT cord-329245-6tj2k1yn 163 52 other other JJ cord-329245-6tj2k1yn 163 53 reason reason NN cord-329245-6tj2k1yn 163 54 ( ( -LRB- cord-329245-6tj2k1yn 163 55 see see VB cord-329245-6tj2k1yn 163 56 Discussion Discussion NNP cord-329245-6tj2k1yn 163 57 ) ) -RRB- cord-329245-6tj2k1yn 163 58 . . . cord-329245-6tj2k1yn 164 1 Thus thus RB cord-329245-6tj2k1yn 164 2 , , , cord-329245-6tj2k1yn 164 3 it -PRON- PRP cord-329245-6tj2k1yn 164 4 is be VBZ cord-329245-6tj2k1yn 164 5 difficult difficult JJ cord-329245-6tj2k1yn 164 6 to to TO cord-329245-6tj2k1yn 164 7 predict predict VB cord-329245-6tj2k1yn 164 8 if if IN cord-329245-6tj2k1yn 164 9 the the DT cord-329245-6tj2k1yn 164 10 E e NN cord-329245-6tj2k1yn 164 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 164 12 tail tail NN cord-329245-6tj2k1yn 164 13 alone alone RB cord-329245-6tj2k1yn 164 14 is be VBZ cord-329245-6tj2k1yn 164 15 capable capable JJ cord-329245-6tj2k1yn 164 16 of of IN cord-329245-6tj2k1yn 164 17 inducing induce VBG cord-329245-6tj2k1yn 164 18 VLPs vlp NNS cord-329245-6tj2k1yn 164 19 . . . cord-329245-6tj2k1yn 165 1 We -PRON- PRP cord-329245-6tj2k1yn 165 2 also also RB cord-329245-6tj2k1yn 165 3 did do VBD cord-329245-6tj2k1yn 165 4 not not RB cord-329245-6tj2k1yn 165 5 observe observe VB cord-329245-6tj2k1yn 165 6 VLP VLP NNP cord-329245-6tj2k1yn 165 7 formation formation NN cord-329245-6tj2k1yn 165 8 with with IN cord-329245-6tj2k1yn 165 9 the the DT cord-329245-6tj2k1yn 165 10 IBV IBV NNP cord-329245-6tj2k1yn 165 11 E E NNP cord-329245-6tj2k1yn 165 12 N N NNP cord-329245-6tj2k1yn 165 13 - - HYPH cord-329245-6tj2k1yn 165 14 terminal terminal NN cord-329245-6tj2k1yn 165 15 truncation truncation NN cord-329245-6tj2k1yn 165 16 mutant mutant JJ cord-329245-6tj2k1yn 165 17 CTE CTE NNP cord-329245-6tj2k1yn 165 18 , , , cord-329245-6tj2k1yn 165 19 which which WDT cord-329245-6tj2k1yn 165 20 consists consist VBZ cord-329245-6tj2k1yn 165 21 only only RB cord-329245-6tj2k1yn 165 22 of of IN cord-329245-6tj2k1yn 165 23 the the DT cord-329245-6tj2k1yn 165 24 E e NN cord-329245-6tj2k1yn 165 25 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 165 26 tail tail NN cord-329245-6tj2k1yn 165 27 ( ( -LRB- cord-329245-6tj2k1yn 165 28 data datum NNS cord-329245-6tj2k1yn 165 29 not not RB cord-329245-6tj2k1yn 165 30 shown show VBN cord-329245-6tj2k1yn 165 31 ) ) -RRB- cord-329245-6tj2k1yn 165 32 . . . cord-329245-6tj2k1yn 166 1 However however RB cord-329245-6tj2k1yn 166 2 , , , cord-329245-6tj2k1yn 166 3 this this DT cord-329245-6tj2k1yn 166 4 result result NN cord-329245-6tj2k1yn 166 5 is be VBZ cord-329245-6tj2k1yn 166 6 inconclusive inconclusive JJ cord-329245-6tj2k1yn 166 7 since since IN cord-329245-6tj2k1yn 166 8 the the DT cord-329245-6tj2k1yn 166 9 CTE CTE NNP cord-329245-6tj2k1yn 166 10 protein protein NN cord-329245-6tj2k1yn 166 11 is be VBZ cord-329245-6tj2k1yn 166 12 less less RBR cord-329245-6tj2k1yn 166 13 stable stable JJ cord-329245-6tj2k1yn 166 14 than than IN cord-329245-6tj2k1yn 166 15 wild wild JJ cord-329245-6tj2k1yn 166 16 - - HYPH cord-329245-6tj2k1yn 166 17 type type NN cord-329245-6tj2k1yn 166 18 E e NN cord-329245-6tj2k1yn 166 19 protein protein NN cord-329245-6tj2k1yn 166 20 and and CC cord-329245-6tj2k1yn 166 21 thus thus RB cord-329245-6tj2k1yn 166 22 accumulates accumulate VBZ cord-329245-6tj2k1yn 166 23 to to IN cord-329245-6tj2k1yn 166 24 significantly significantly RB cord-329245-6tj2k1yn 166 25 lower low JJR cord-329245-6tj2k1yn 166 26 levels level NNS cord-329245-6tj2k1yn 166 27 ( ( -LRB- cord-329245-6tj2k1yn 166 28 Corse Corse NNP cord-329245-6tj2k1yn 166 29 and and CC cord-329245-6tj2k1yn 166 30 Machamer Machamer NNP cord-329245-6tj2k1yn 166 31 , , , cord-329245-6tj2k1yn 166 32 2002 2002 CD cord-329245-6tj2k1yn 166 33 ) ) -RRB- cord-329245-6tj2k1yn 166 34 . . . cord-329245-6tj2k1yn 167 1 We -PRON- PRP cord-329245-6tj2k1yn 167 2 evaluated evaluate VBD cord-329245-6tj2k1yn 167 3 the the DT cord-329245-6tj2k1yn 167 4 incorporation incorporation NN cord-329245-6tj2k1yn 167 5 of of IN cord-329245-6tj2k1yn 167 6 the the DT cord-329245-6tj2k1yn 167 7 IBV IBV NNP cord-329245-6tj2k1yn 167 8 M M NNP cord-329245-6tj2k1yn 167 9 deletion deletion NN cord-329245-6tj2k1yn 167 10 mutants mutant NNS cord-329245-6tj2k1yn 167 11 Mct mct VBP cord-329245-6tj2k1yn 167 12 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 167 13 1 1 CD cord-329245-6tj2k1yn 167 14 , , , cord-329245-6tj2k1yn 167 15 Mct Mct NNP cord-329245-6tj2k1yn 167 16 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 167 17 2 2 CD cord-329245-6tj2k1yn 167 18 , , , cord-329245-6tj2k1yn 167 19 Mct Mct NNP cord-329245-6tj2k1yn 167 20 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 167 21 3 3 CD cord-329245-6tj2k1yn 167 22 , , , cord-329245-6tj2k1yn 167 23 and and CC cord-329245-6tj2k1yn 167 24 M M NNP cord-329245-6tj2k1yn 167 25 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 167 26 m2,3 m2,3 NNP cord-329245-6tj2k1yn 167 27 into into IN cord-329245-6tj2k1yn 167 28 VLPs vlp NNS cord-329245-6tj2k1yn 167 29 . . . cord-329245-6tj2k1yn 168 1 As as IN cord-329245-6tj2k1yn 168 2 shown show VBN cord-329245-6tj2k1yn 168 3 in in IN cord-329245-6tj2k1yn 168 4 Fig Fig NNP cord-329245-6tj2k1yn 168 5 . . . cord-329245-6tj2k1yn 169 1 7 7 CD cord-329245-6tj2k1yn 169 2 , , , cord-329245-6tj2k1yn 169 3 none none NN cord-329245-6tj2k1yn 169 4 of of IN cord-329245-6tj2k1yn 169 5 the the DT cord-329245-6tj2k1yn 169 6 deletion deletion NN cord-329245-6tj2k1yn 169 7 mutants mutant NNS cord-329245-6tj2k1yn 169 8 were be VBD cord-329245-6tj2k1yn 169 9 able able JJ cord-329245-6tj2k1yn 169 10 to to TO cord-329245-6tj2k1yn 169 11 support support VB cord-329245-6tj2k1yn 169 12 VLP VLP NNP cord-329245-6tj2k1yn 169 13 formation formation NN cord-329245-6tj2k1yn 169 14 at at IN cord-329245-6tj2k1yn 169 15 a a DT cord-329245-6tj2k1yn 169 16 detectable detectable JJ cord-329245-6tj2k1yn 169 17 level level NN cord-329245-6tj2k1yn 169 18 , , , cord-329245-6tj2k1yn 169 19 regardless regardless RB cord-329245-6tj2k1yn 169 20 of of IN cord-329245-6tj2k1yn 169 21 whether whether IN cord-329245-6tj2k1yn 169 22 they -PRON- PRP cord-329245-6tj2k1yn 169 23 could could MD cord-329245-6tj2k1yn 169 24 be be VB cord-329245-6tj2k1yn 169 25 crosslinked crosslinke VBN cord-329245-6tj2k1yn 169 26 to to IN cord-329245-6tj2k1yn 169 27 IBV IBV NNP cord-329245-6tj2k1yn 169 28 E. E. NNP cord-329245-6tj2k1yn 170 1 The the DT cord-329245-6tj2k1yn 170 2 Mct Mct NNP cord-329245-6tj2k1yn 170 3 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 170 4 1 1 CD cord-329245-6tj2k1yn 170 5 and and CC cord-329245-6tj2k1yn 170 6 Mct Mct NNP cord-329245-6tj2k1yn 170 7 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 170 8 2 2 CD cord-329245-6tj2k1yn 170 9 mutants mutant NNS cord-329245-6tj2k1yn 170 10 are be VBP cord-329245-6tj2k1yn 170 11 not not RB cord-329245-6tj2k1yn 170 12 radiolabeled radiolabele VBN cord-329245-6tj2k1yn 170 13 as as RB cord-329245-6tj2k1yn 170 14 efficiently efficiently RB cord-329245-6tj2k1yn 170 15 as as IN cord-329245-6tj2k1yn 170 16 wildtype wildtype NN cord-329245-6tj2k1yn 170 17 M M NNP cord-329245-6tj2k1yn 170 18 and and CC cord-329245-6tj2k1yn 170 19 the the DT cord-329245-6tj2k1yn 170 20 Mct Mct NNP cord-329245-6tj2k1yn 170 21 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 170 22 3 3 CD cord-329245-6tj2k1yn 170 23 and and CC cord-329245-6tj2k1yn 170 24 M M NNP cord-329245-6tj2k1yn 170 25 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 170 26 m2,3 m2,3 NNP cord-329245-6tj2k1yn 170 27 mutants mutant NNS cord-329245-6tj2k1yn 170 28 ( ( -LRB- cord-329245-6tj2k1yn 170 29 compare compare VB cord-329245-6tj2k1yn 170 30 lanes lane NNS cord-329245-6tj2k1yn 170 31 5,6,7 5,6,7 CD cord-329245-6tj2k1yn 170 32 , , , cord-329245-6tj2k1yn 170 33 and and CC cord-329245-6tj2k1yn 170 34 8 8 CD cord-329245-6tj2k1yn 170 35 with with IN cord-329245-6tj2k1yn 170 36 lanes lane NNS cord-329245-6tj2k1yn 170 37 2 2 CD cord-329245-6tj2k1yn 170 38 and and CC cord-329245-6tj2k1yn 170 39 3 3 LS cord-329245-6tj2k1yn 170 40 ) ) -RRB- cord-329245-6tj2k1yn 170 41 because because IN cord-329245-6tj2k1yn 170 42 they -PRON- PRP cord-329245-6tj2k1yn 170 43 have have VBP cord-329245-6tj2k1yn 170 44 fewer few JJR cord-329245-6tj2k1yn 170 45 methionines methionine NNS cord-329245-6tj2k1yn 170 46 and and CC cord-329245-6tj2k1yn 170 47 cysteines cysteine NNS cord-329245-6tj2k1yn 170 48 due due IN cord-329245-6tj2k1yn 170 49 to to IN cord-329245-6tj2k1yn 170 50 their -PRON- PRP$ cord-329245-6tj2k1yn 170 51 deletions deletion NNS cord-329245-6tj2k1yn 170 52 . . . cord-329245-6tj2k1yn 171 1 However however RB cord-329245-6tj2k1yn 171 2 , , , cord-329245-6tj2k1yn 171 3 we -PRON- PRP cord-329245-6tj2k1yn 171 4 would would MD cord-329245-6tj2k1yn 171 5 still still RB cord-329245-6tj2k1yn 171 6 expect expect VB cord-329245-6tj2k1yn 171 7 to to TO cord-329245-6tj2k1yn 171 8 see see VB cord-329245-6tj2k1yn 171 9 a a DT cord-329245-6tj2k1yn 171 10 detectable detectable JJ cord-329245-6tj2k1yn 171 11 signal signal NN cord-329245-6tj2k1yn 171 12 in in IN cord-329245-6tj2k1yn 171 13 the the DT cord-329245-6tj2k1yn 171 14 supernatants supernatant NNS cord-329245-6tj2k1yn 171 15 after after IN cord-329245-6tj2k1yn 171 16 a a DT cord-329245-6tj2k1yn 171 17 long long JJ cord-329245-6tj2k1yn 171 18 exposure exposure NN cord-329245-6tj2k1yn 171 19 if if IN cord-329245-6tj2k1yn 171 20 these these DT cord-329245-6tj2k1yn 171 21 mutants mutant NNS cord-329245-6tj2k1yn 171 22 supported support VBD cord-329245-6tj2k1yn 171 23 VLP VLP NNP cord-329245-6tj2k1yn 171 24 formation formation NN cord-329245-6tj2k1yn 171 25 at at IN cord-329245-6tj2k1yn 171 26 a a DT cord-329245-6tj2k1yn 171 27 level level NN cord-329245-6tj2k1yn 171 28 comparable comparable JJ cord-329245-6tj2k1yn 171 29 to to IN cord-329245-6tj2k1yn 171 30 wild wild JJ cord-329245-6tj2k1yn 171 31 - - HYPH cord-329245-6tj2k1yn 171 32 type type NN cord-329245-6tj2k1yn 171 33 M. m. NN cord-329245-6tj2k1yn 172 1 Since since IN cord-329245-6tj2k1yn 172 2 we -PRON- PRP cord-329245-6tj2k1yn 172 3 have have VBP cord-329245-6tj2k1yn 172 4 never never RB cord-329245-6tj2k1yn 172 5 seen see VBN cord-329245-6tj2k1yn 172 6 such such PDT cord-329245-6tj2k1yn 172 7 a a DT cord-329245-6tj2k1yn 172 8 signal signal NN cord-329245-6tj2k1yn 172 9 , , , cord-329245-6tj2k1yn 172 10 we -PRON- PRP cord-329245-6tj2k1yn 172 11 conclude conclude VBP cord-329245-6tj2k1yn 172 12 that that IN cord-329245-6tj2k1yn 172 13 these these DT cord-329245-6tj2k1yn 172 14 mutants mutant NNS cord-329245-6tj2k1yn 172 15 do do VBP cord-329245-6tj2k1yn 172 16 not not RB cord-329245-6tj2k1yn 172 17 support support VB cord-329245-6tj2k1yn 172 18 VLP VLP NNP cord-329245-6tj2k1yn 172 19 formation formation NN cord-329245-6tj2k1yn 172 20 . . . cord-329245-6tj2k1yn 173 1 These these DT cord-329245-6tj2k1yn 173 2 results result NNS cord-329245-6tj2k1yn 173 3 indicate indicate VBP cord-329245-6tj2k1yn 173 4 that that IN cord-329245-6tj2k1yn 173 5 both both CC cord-329245-6tj2k1yn 173 6 the the DT cord-329245-6tj2k1yn 173 7 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 173 8 tail tail NN cord-329245-6tj2k1yn 173 9 and and CC cord-329245-6tj2k1yn 173 10 the the DT cord-329245-6tj2k1yn 173 11 transmembrane transmembrane NN cord-329245-6tj2k1yn 173 12 regions region NNS cord-329245-6tj2k1yn 173 13 of of IN cord-329245-6tj2k1yn 173 14 IBV IBV NNP cord-329245-6tj2k1yn 173 15 M M NNP cord-329245-6tj2k1yn 173 16 are be VBP cord-329245-6tj2k1yn 173 17 involved involve VBN cord-329245-6tj2k1yn 173 18 in in IN cord-329245-6tj2k1yn 173 19 VLP VLP NNP cord-329245-6tj2k1yn 173 20 formation formation NN cord-329245-6tj2k1yn 173 21 , , , cord-329245-6tj2k1yn 173 22 which which WDT cord-329245-6tj2k1yn 173 23 is be VBZ cord-329245-6tj2k1yn 173 24 consistent consistent JJ cord-329245-6tj2k1yn 173 25 with with IN cord-329245-6tj2k1yn 173 26 the the DT cord-329245-6tj2k1yn 173 27 results result NNS cord-329245-6tj2k1yn 173 28 of of IN cord-329245-6tj2k1yn 173 29 Rottier Rottier NNP cord-329245-6tj2k1yn 173 30 and and CC cord-329245-6tj2k1yn 173 31 colleagues colleague NNS cord-329245-6tj2k1yn 173 32 , , , cord-329245-6tj2k1yn 173 33 who who WP cord-329245-6tj2k1yn 173 34 found find VBD cord-329245-6tj2k1yn 173 35 that that IN cord-329245-6tj2k1yn 173 36 mutations mutation NNS cord-329245-6tj2k1yn 173 37 in in IN cord-329245-6tj2k1yn 173 38 all all DT cord-329245-6tj2k1yn 173 39 domains domain NNS cord-329245-6tj2k1yn 173 40 of of IN cord-329245-6tj2k1yn 173 41 the the DT cord-329245-6tj2k1yn 173 42 MHV MHV NNP cord-329245-6tj2k1yn 173 43 M M NNP cord-329245-6tj2k1yn 173 44 protein protein NN cord-329245-6tj2k1yn 173 45 abrogated abrogate VBD cord-329245-6tj2k1yn 173 46 VLP VLP NNP cord-329245-6tj2k1yn 173 47 formation formation NN cord-329245-6tj2k1yn 173 48 ( ( -LRB- cord-329245-6tj2k1yn 173 49 De De NNP cord-329245-6tj2k1yn 173 50 Haan Haan NNP cord-329245-6tj2k1yn 173 51 et et FW cord-329245-6tj2k1yn 173 52 al al NNP cord-329245-6tj2k1yn 173 53 . . NNP cord-329245-6tj2k1yn 173 54 , , , cord-329245-6tj2k1yn 173 55 1998 1998 CD cord-329245-6tj2k1yn 173 56 ) ) -RRB- cord-329245-6tj2k1yn 173 57 . . . cord-329245-6tj2k1yn 174 1 These these DT cord-329245-6tj2k1yn 174 2 results result NNS cord-329245-6tj2k1yn 174 3 suggest suggest VBP cord-329245-6tj2k1yn 174 4 that that IN cord-329245-6tj2k1yn 174 5 a a DT cord-329245-6tj2k1yn 174 6 direct direct JJ cord-329245-6tj2k1yn 174 7 interaction interaction NN cord-329245-6tj2k1yn 174 8 between between IN cord-329245-6tj2k1yn 174 9 IBV IBV NNP cord-329245-6tj2k1yn 174 10 E E NNP cord-329245-6tj2k1yn 174 11 and and CC cord-329245-6tj2k1yn 174 12 M M NNP cord-329245-6tj2k1yn 174 13 proteins protein NNS cord-329245-6tj2k1yn 174 14 is be VBZ cord-329245-6tj2k1yn 174 15 not not RB cord-329245-6tj2k1yn 174 16 sufficient sufficient JJ cord-329245-6tj2k1yn 174 17 for for IN cord-329245-6tj2k1yn 174 18 VLP VLP NNP cord-329245-6tj2k1yn 174 19 formation formation NN cord-329245-6tj2k1yn 174 20 and and CC cord-329245-6tj2k1yn 174 21 that that IN cord-329245-6tj2k1yn 174 22 this this DT cord-329245-6tj2k1yn 174 23 process process NN cord-329245-6tj2k1yn 174 24 may may MD cord-329245-6tj2k1yn 174 25 depend depend VB cord-329245-6tj2k1yn 174 26 on on IN cord-329245-6tj2k1yn 174 27 how how WRB cord-329245-6tj2k1yn 174 28 the the DT cord-329245-6tj2k1yn 174 29 two two CD cord-329245-6tj2k1yn 174 30 proteins protein NNS cord-329245-6tj2k1yn 174 31 interact interact VBP cord-329245-6tj2k1yn 174 32 with with IN cord-329245-6tj2k1yn 174 33 the the DT cord-329245-6tj2k1yn 174 34 membrane membrane NN cord-329245-6tj2k1yn 174 35 bilayer bilayer NN cord-329245-6tj2k1yn 174 36 , , , cord-329245-6tj2k1yn 174 37 both both CC cord-329245-6tj2k1yn 174 38 separately separately RB cord-329245-6tj2k1yn 174 39 and and CC cord-329245-6tj2k1yn 174 40 together together RB cord-329245-6tj2k1yn 174 41 . . . cord-329245-6tj2k1yn 175 1 We -PRON- PRP cord-329245-6tj2k1yn 175 2 have have VBP cord-329245-6tj2k1yn 175 3 demonstrated demonstrate VBN cord-329245-6tj2k1yn 175 4 an an DT cord-329245-6tj2k1yn 175 5 interaction interaction NN cord-329245-6tj2k1yn 175 6 between between IN cord-329245-6tj2k1yn 175 7 the the DT cord-329245-6tj2k1yn 175 8 IBV IBV NNP cord-329245-6tj2k1yn 175 9 E E NNP cord-329245-6tj2k1yn 175 10 and and CC cord-329245-6tj2k1yn 175 11 M M NNP cord-329245-6tj2k1yn 175 12 proteins protein NNS cord-329245-6tj2k1yn 175 13 using use VBG cord-329245-6tj2k1yn 175 14 both both CC cord-329245-6tj2k1yn 175 15 in in IN cord-329245-6tj2k1yn 175 16 vivo vivo NN cord-329245-6tj2k1yn 175 17 chemical chemical NN cord-329245-6tj2k1yn 175 18 crosslinking crosslinking NN cord-329245-6tj2k1yn 175 19 and and CC cord-329245-6tj2k1yn 175 20 VLP VLP NNP cord-329245-6tj2k1yn 175 21 formation formation NN cord-329245-6tj2k1yn 175 22 as as IN cord-329245-6tj2k1yn 175 23 assays assay NNS cord-329245-6tj2k1yn 175 24 . . . cord-329245-6tj2k1yn 176 1 Using use VBG cord-329245-6tj2k1yn 176 2 IBV IBV NNP cord-329245-6tj2k1yn 176 3 E E NNP cord-329245-6tj2k1yn 176 4 and and CC cord-329245-6tj2k1yn 176 5 M M NNP cord-329245-6tj2k1yn 176 6 protein protein NN cord-329245-6tj2k1yn 176 7 mutants mutant NNS cord-329245-6tj2k1yn 176 8 that that WDT cord-329245-6tj2k1yn 176 9 were be VBD cord-329245-6tj2k1yn 176 10 generated generate VBN cord-329245-6tj2k1yn 176 11 in in IN cord-329245-6tj2k1yn 176 12 the the DT cord-329245-6tj2k1yn 176 13 course course NN cord-329245-6tj2k1yn 176 14 of of IN cord-329245-6tj2k1yn 176 15 Golgi Golgi NNP cord-329245-6tj2k1yn 176 16 targeting target VBG cord-329245-6tj2k1yn 176 17 studies study NNS cord-329245-6tj2k1yn 176 18 , , , cord-329245-6tj2k1yn 176 19 we -PRON- PRP cord-329245-6tj2k1yn 176 20 examined examine VBD cord-329245-6tj2k1yn 176 21 the the DT cord-329245-6tj2k1yn 176 22 domains domain NNS cord-329245-6tj2k1yn 176 23 in in IN cord-329245-6tj2k1yn 176 24 the the DT cord-329245-6tj2k1yn 176 25 IBV IBV NNP cord-329245-6tj2k1yn 176 26 E E NNP cord-329245-6tj2k1yn 176 27 and and CC cord-329245-6tj2k1yn 176 28 M M NNP cord-329245-6tj2k1yn 176 29 proteins protein NNS cord-329245-6tj2k1yn 176 30 that that WDT cord-329245-6tj2k1yn 176 31 are be VBP cord-329245-6tj2k1yn 176 32 involved involve VBN cord-329245-6tj2k1yn 176 33 in in IN cord-329245-6tj2k1yn 176 34 their -PRON- PRP$ cord-329245-6tj2k1yn 176 35 interaction interaction NN cord-329245-6tj2k1yn 176 36 . . . cord-329245-6tj2k1yn 177 1 We -PRON- PRP cord-329245-6tj2k1yn 177 2 found find VBD cord-329245-6tj2k1yn 177 3 that that IN cord-329245-6tj2k1yn 177 4 the the DT cord-329245-6tj2k1yn 177 5 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 177 6 tails tail NNS cord-329245-6tj2k1yn 177 7 of of IN cord-329245-6tj2k1yn 177 8 both both DT cord-329245-6tj2k1yn 177 9 proteins protein NNS cord-329245-6tj2k1yn 177 10 mediate mediate VBP cord-329245-6tj2k1yn 177 11 their -PRON- PRP$ cord-329245-6tj2k1yn 177 12 interaction interaction NN cord-329245-6tj2k1yn 177 13 , , , cord-329245-6tj2k1yn 177 14 as as IN cord-329245-6tj2k1yn 177 15 measured measure VBN cord-329245-6tj2k1yn 177 16 by by IN cord-329245-6tj2k1yn 177 17 crosslinking crosslinke VBG cord-329245-6tj2k1yn 177 18 . . . cord-329245-6tj2k1yn 178 1 Interestingly interestingly RB cord-329245-6tj2k1yn 178 2 , , , cord-329245-6tj2k1yn 178 3 however however RB cord-329245-6tj2k1yn 178 4 , , , cord-329245-6tj2k1yn 178 5 we -PRON- PRP cord-329245-6tj2k1yn 178 6 observed observe VBD cord-329245-6tj2k1yn 178 7 that that IN cord-329245-6tj2k1yn 178 8 reciprocal reciprocal JJ cord-329245-6tj2k1yn 178 9 crosslinking crosslinking NN cord-329245-6tj2k1yn 178 10 of of IN cord-329245-6tj2k1yn 178 11 IBV IBV NNP cord-329245-6tj2k1yn 178 12 E E NNP cord-329245-6tj2k1yn 178 13 and and CC cord-329245-6tj2k1yn 178 14 M M NNP cord-329245-6tj2k1yn 178 15 protein protein NN cord-329245-6tj2k1yn 178 16 mutants mutant NNS cord-329245-6tj2k1yn 178 17 did do VBD cord-329245-6tj2k1yn 178 18 not not RB cord-329245-6tj2k1yn 178 19 correlate correlate VB cord-329245-6tj2k1yn 178 20 with with IN cord-329245-6tj2k1yn 178 21 VLP VLP NNP cord-329245-6tj2k1yn 178 22 formation formation NN cord-329245-6tj2k1yn 178 23 , , , cord-329245-6tj2k1yn 178 24 suggesting suggest VBG cord-329245-6tj2k1yn 178 25 that that IN cord-329245-6tj2k1yn 178 26 interaction interaction NN cord-329245-6tj2k1yn 178 27 between between IN cord-329245-6tj2k1yn 178 28 IBV IBV NNP cord-329245-6tj2k1yn 178 29 E E NNP cord-329245-6tj2k1yn 178 30 and and CC cord-329245-6tj2k1yn 178 31 M M NNP cord-329245-6tj2k1yn 178 32 is be VBZ cord-329245-6tj2k1yn 178 33 not not RB cord-329245-6tj2k1yn 178 34 sufficient sufficient JJ cord-329245-6tj2k1yn 178 35 for for IN cord-329245-6tj2k1yn 178 36 VLP VLP NNP cord-329245-6tj2k1yn 178 37 formation formation NN cord-329245-6tj2k1yn 178 38 . . . cord-329245-6tj2k1yn 179 1 Here here RB cord-329245-6tj2k1yn 179 2 we -PRON- PRP cord-329245-6tj2k1yn 179 3 compare compare VBP cord-329245-6tj2k1yn 179 4 our -PRON- PRP$ cord-329245-6tj2k1yn 179 5 results result NNS cord-329245-6tj2k1yn 179 6 to to IN cord-329245-6tj2k1yn 179 7 those those DT cord-329245-6tj2k1yn 179 8 reported report VBN cord-329245-6tj2k1yn 179 9 by by IN cord-329245-6tj2k1yn 179 10 other other JJ cord-329245-6tj2k1yn 179 11 groups group NNS cord-329245-6tj2k1yn 179 12 and and CC cord-329245-6tj2k1yn 179 13 discuss discuss VB cord-329245-6tj2k1yn 179 14 the the DT cord-329245-6tj2k1yn 179 15 implications implication NNS cord-329245-6tj2k1yn 179 16 of of IN cord-329245-6tj2k1yn 179 17 our -PRON- PRP$ cord-329245-6tj2k1yn 179 18 findings finding NNS cord-329245-6tj2k1yn 179 19 for for IN cord-329245-6tj2k1yn 179 20 coronavirus coronavirus NN cord-329245-6tj2k1yn 179 21 assembly assembly NN cord-329245-6tj2k1yn 179 22 . . . cord-329245-6tj2k1yn 180 1 Lim Lim NNP cord-329245-6tj2k1yn 180 2 and and CC cord-329245-6tj2k1yn 180 3 Liu Liu NNP cord-329245-6tj2k1yn 180 4 previously previously RB cord-329245-6tj2k1yn 180 5 used use VBD cord-329245-6tj2k1yn 180 6 a a DT cord-329245-6tj2k1yn 180 7 coimmunoprecipitation coimmunoprecipitation NN cord-329245-6tj2k1yn 180 8 assay assay NN cord-329245-6tj2k1yn 180 9 to to TO cord-329245-6tj2k1yn 180 10 detect detect VB cord-329245-6tj2k1yn 180 11 IBV IBV NNP cord-329245-6tj2k1yn 180 12 E E NNP cord-329245-6tj2k1yn 180 13 and and CC cord-329245-6tj2k1yn 180 14 M M NNP cord-329245-6tj2k1yn 180 15 protein protein NN cord-329245-6tj2k1yn 180 16 interactions interaction NNS cord-329245-6tj2k1yn 180 17 ( ( -LRB- cord-329245-6tj2k1yn 180 18 Lim Lim NNP cord-329245-6tj2k1yn 180 19 and and CC cord-329245-6tj2k1yn 180 20 Liu Liu NNP cord-329245-6tj2k1yn 180 21 , , , cord-329245-6tj2k1yn 180 22 2001 2001 CD cord-329245-6tj2k1yn 180 23 ) ) -RRB- cord-329245-6tj2k1yn 180 24 . . . cord-329245-6tj2k1yn 181 1 They -PRON- PRP cord-329245-6tj2k1yn 181 2 also also RB cord-329245-6tj2k1yn 181 3 concluded conclude VBD cord-329245-6tj2k1yn 181 4 that that IN cord-329245-6tj2k1yn 181 5 the the DT cord-329245-6tj2k1yn 181 6 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 181 7 tail tail NN cord-329245-6tj2k1yn 181 8 of of IN cord-329245-6tj2k1yn 181 9 IBV IBV NNP cord-329245-6tj2k1yn 181 10 E E NNP cord-329245-6tj2k1yn 181 11 is be VBZ cord-329245-6tj2k1yn 181 12 important important JJ cord-329245-6tj2k1yn 181 13 for for IN cord-329245-6tj2k1yn 181 14 interaction interaction NN cord-329245-6tj2k1yn 181 15 with with IN cord-329245-6tj2k1yn 181 16 IBV IBV NNP cord-329245-6tj2k1yn 181 17 M M NNP cord-329245-6tj2k1yn 181 18 , , , cord-329245-6tj2k1yn 181 19 since since IN cord-329245-6tj2k1yn 181 20 dele dele NNP cord-329245-6tj2k1yn 181 21 - - HYPH cord-329245-6tj2k1yn 181 22 tion tion NNP cord-329245-6tj2k1yn 181 23 of of IN cord-329245-6tj2k1yn 181 24 a a DT cord-329245-6tj2k1yn 181 25 20 20 CD cord-329245-6tj2k1yn 181 26 amino amino NN cord-329245-6tj2k1yn 181 27 acid acid NN cord-329245-6tj2k1yn 181 28 region region NN cord-329245-6tj2k1yn 181 29 within within IN cord-329245-6tj2k1yn 181 30 the the DT cord-329245-6tj2k1yn 181 31 E e NN cord-329245-6tj2k1yn 181 32 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 181 33 tail tail NN cord-329245-6tj2k1yn 181 34 ( ( -LRB- cord-329245-6tj2k1yn 181 35 amino amino JJ cord-329245-6tj2k1yn 181 36 acids acid NNS cord-329245-6tj2k1yn 181 37 37 37 CD cord-329245-6tj2k1yn 181 38 - - SYM cord-329245-6tj2k1yn 181 39 57 57 CD cord-329245-6tj2k1yn 181 40 ) ) -RRB- cord-329245-6tj2k1yn 181 41 prevented prevent VBD cord-329245-6tj2k1yn 181 42 coimmunoprecipitation coimmunoprecipitation NN cord-329245-6tj2k1yn 181 43 of of IN cord-329245-6tj2k1yn 181 44 the the DT cord-329245-6tj2k1yn 181 45 E E NNP cord-329245-6tj2k1yn 181 46 and and CC cord-329245-6tj2k1yn 181 47 M M NNP cord-329245-6tj2k1yn 181 48 proteins protein NNS cord-329245-6tj2k1yn 181 49 and and CC cord-329245-6tj2k1yn 181 50 VLP VLP NNP cord-329245-6tj2k1yn 181 51 formation formation NN cord-329245-6tj2k1yn 181 52 . . . cord-329245-6tj2k1yn 182 1 As as IN cord-329245-6tj2k1yn 182 2 mentioned mention VBN cord-329245-6tj2k1yn 182 3 above above RB cord-329245-6tj2k1yn 182 4 , , , cord-329245-6tj2k1yn 182 5 we -PRON- PRP cord-329245-6tj2k1yn 182 6 did do VBD cord-329245-6tj2k1yn 182 7 not not RB cord-329245-6tj2k1yn 182 8 attempt attempt VB cord-329245-6tj2k1yn 182 9 to to TO cord-329245-6tj2k1yn 182 10 find find VB cord-329245-6tj2k1yn 182 11 a a DT cord-329245-6tj2k1yn 182 12 region region NN cord-329245-6tj2k1yn 182 13 within within IN cord-329245-6tj2k1yn 182 14 the the DT cord-329245-6tj2k1yn 182 15 E e NN cord-329245-6tj2k1yn 182 16 tail tail NN cord-329245-6tj2k1yn 182 17 that that WDT cord-329245-6tj2k1yn 182 18 mediates mediate VBZ cord-329245-6tj2k1yn 182 19 its -PRON- PRP$ cord-329245-6tj2k1yn 182 20 interaction interaction NN cord-329245-6tj2k1yn 182 21 with with IN cord-329245-6tj2k1yn 182 22 IBV IBV NNP cord-329245-6tj2k1yn 182 23 M M NNP cord-329245-6tj2k1yn 182 24 , , , cord-329245-6tj2k1yn 182 25 because because IN cord-329245-6tj2k1yn 182 26 we -PRON- PRP cord-329245-6tj2k1yn 182 27 previously previously RB cord-329245-6tj2k1yn 182 28 found find VBD cord-329245-6tj2k1yn 182 29 that that IN cord-329245-6tj2k1yn 182 30 partial partial JJ cord-329245-6tj2k1yn 182 31 or or CC cord-329245-6tj2k1yn 182 32 complete complete JJ cord-329245-6tj2k1yn 182 33 replacement replacement NN cord-329245-6tj2k1yn 182 34 of of IN cord-329245-6tj2k1yn 182 35 a a DT cord-329245-6tj2k1yn 182 36 50 50 CD cord-329245-6tj2k1yn 182 37 amino amino NN cord-329245-6tj2k1yn 182 38 acid acid NN cord-329245-6tj2k1yn 182 39 region region NN cord-329245-6tj2k1yn 182 40 in in IN cord-329245-6tj2k1yn 182 41 the the DT cord-329245-6tj2k1yn 182 42 IBV IBV NNP cord-329245-6tj2k1yn 182 43 E e NN cord-329245-6tj2k1yn 182 44 tail tail NN cord-329245-6tj2k1yn 182 45 results result NNS cord-329245-6tj2k1yn 182 46 in in IN cord-329245-6tj2k1yn 182 47 transport transport NN cord-329245-6tj2k1yn 182 48 of of IN cord-329245-6tj2k1yn 182 49 a a DT cord-329245-6tj2k1yn 182 50 reporter reporter NN cord-329245-6tj2k1yn 182 51 chimera chimera NN cord-329245-6tj2k1yn 182 52 from from IN cord-329245-6tj2k1yn 182 53 the the DT cord-329245-6tj2k1yn 182 54 Golgi Golgi NNP cord-329245-6tj2k1yn 182 55 complex complex NN cord-329245-6tj2k1yn 182 56 to to IN cord-329245-6tj2k1yn 182 57 the the DT cord-329245-6tj2k1yn 182 58 cell cell NN cord-329245-6tj2k1yn 182 59 surface surface NN cord-329245-6tj2k1yn 182 60 , , , cord-329245-6tj2k1yn 182 61 which which WDT cord-329245-6tj2k1yn 182 62 indicates indicate VBZ cord-329245-6tj2k1yn 182 63 that that IN cord-329245-6tj2k1yn 182 64 the the DT cord-329245-6tj2k1yn 182 65 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 182 66 tail tail NN cord-329245-6tj2k1yn 182 67 of of IN cord-329245-6tj2k1yn 182 68 E e NN cord-329245-6tj2k1yn 182 69 contains contain VBZ cord-329245-6tj2k1yn 182 70 Golgi Golgi NNP cord-329245-6tj2k1yn 182 71 targeting target VBG cord-329245-6tj2k1yn 182 72 information information NN cord-329245-6tj2k1yn 182 73 ( ( -LRB- cord-329245-6tj2k1yn 182 74 Corse Corse NNP cord-329245-6tj2k1yn 182 75 and and CC cord-329245-6tj2k1yn 182 76 Machamer Machamer NNP cord-329245-6tj2k1yn 182 77 , , , cord-329245-6tj2k1yn 182 78 2002 2002 CD cord-329245-6tj2k1yn 182 79 ) ) -RRB- cord-329245-6tj2k1yn 182 80 . . . cord-329245-6tj2k1yn 183 1 Our -PRON- PRP$ cord-329245-6tj2k1yn 183 2 subcellular subcellular JJ cord-329245-6tj2k1yn 183 3 localization localization NN cord-329245-6tj2k1yn 183 4 results result NNS cord-329245-6tj2k1yn 183 5 differ differ VBP cord-329245-6tj2k1yn 183 6 from from IN cord-329245-6tj2k1yn 183 7 those those DT cord-329245-6tj2k1yn 183 8 of of IN cord-329245-6tj2k1yn 183 9 Lim Lim NNP cord-329245-6tj2k1yn 183 10 and and CC cord-329245-6tj2k1yn 183 11 Liu Liu NNP cord-329245-6tj2k1yn 183 12 , , , cord-329245-6tj2k1yn 183 13 who who WP cord-329245-6tj2k1yn 183 14 reported report VBD cord-329245-6tj2k1yn 183 15 that that IN cord-329245-6tj2k1yn 183 16 IBV IBV NNP cord-329245-6tj2k1yn 183 17 E E NNP cord-329245-6tj2k1yn 183 18 is be VBZ cord-329245-6tj2k1yn 183 19 localized localize VBN cord-329245-6tj2k1yn 183 20 to to IN cord-329245-6tj2k1yn 183 21 the the DT cord-329245-6tj2k1yn 183 22 endoplasmic endoplasmic NN cord-329245-6tj2k1yn 183 23 reticulum reticulum NN cord-329245-6tj2k1yn 183 24 of of IN cord-329245-6tj2k1yn 183 25 transfected transfecte VBN cord-329245-6tj2k1yn 183 26 cells cell NNS cord-329245-6tj2k1yn 183 27 ( ( -LRB- cord-329245-6tj2k1yn 183 28 Lim Lim NNP cord-329245-6tj2k1yn 183 29 and and CC cord-329245-6tj2k1yn 183 30 Liu Liu NNP cord-329245-6tj2k1yn 183 31 , , , cord-329245-6tj2k1yn 183 32 2001 2001 CD cord-329245-6tj2k1yn 183 33 ) ) -RRB- cord-329245-6tj2k1yn 183 34 , , , cord-329245-6tj2k1yn 183 35 possibly possibly RB cord-329245-6tj2k1yn 183 36 because because IN cord-329245-6tj2k1yn 183 37 they -PRON- PRP cord-329245-6tj2k1yn 183 38 examined examine VBD cord-329245-6tj2k1yn 183 39 cells cell NNS cord-329245-6tj2k1yn 183 40 overexpressing overexpresse VBG cord-329245-6tj2k1yn 183 41 epitope epitope NN cord-329245-6tj2k1yn 183 42 - - HYPH cord-329245-6tj2k1yn 183 43 tagged tag VBN cord-329245-6tj2k1yn 183 44 IBV IBV NNP cord-329245-6tj2k1yn 183 45 E E NNP cord-329245-6tj2k1yn 183 46 protein protein NN cord-329245-6tj2k1yn 183 47 . . . cord-329245-6tj2k1yn 184 1 The the DT cord-329245-6tj2k1yn 184 2 M M NNP cord-329245-6tj2k1yn 184 3 protein protein NN cord-329245-6tj2k1yn 184 4 is be VBZ cord-329245-6tj2k1yn 184 5 by by IN cord-329245-6tj2k1yn 184 6 far far RB cord-329245-6tj2k1yn 184 7 the the DT cord-329245-6tj2k1yn 184 8 most most RBS cord-329245-6tj2k1yn 184 9 abundant abundant JJ cord-329245-6tj2k1yn 184 10 envelope envelope NN cord-329245-6tj2k1yn 184 11 protein protein NN cord-329245-6tj2k1yn 184 12 of of IN cord-329245-6tj2k1yn 184 13 coronaviruses coronaviruse NNS cord-329245-6tj2k1yn 184 14 and and CC cord-329245-6tj2k1yn 184 15 has have VBZ cord-329245-6tj2k1yn 184 16 been be VBN cord-329245-6tj2k1yn 184 17 estimated estimate VBN cord-329245-6tj2k1yn 184 18 to to TO cord-329245-6tj2k1yn 184 19 account account VB cord-329245-6tj2k1yn 184 20 for for IN cord-329245-6tj2k1yn 184 21 ϳ40 ϳ40 CD cord-329245-6tj2k1yn 184 22 % % NN cord-329245-6tj2k1yn 184 23 of of IN cord-329245-6tj2k1yn 184 24 the the DT cord-329245-6tj2k1yn 184 25 mass mass NN cord-329245-6tj2k1yn 184 26 of of IN cord-329245-6tj2k1yn 184 27 IBV IBV NNP cord-329245-6tj2k1yn 184 28 and and CC cord-329245-6tj2k1yn 184 29 MHV MHV NNP cord-329245-6tj2k1yn 184 30 particles particle NNS cord-329245-6tj2k1yn 185 1 ( ( -LRB- cord-329245-6tj2k1yn 185 2 Stern Stern NNP cord-329245-6tj2k1yn 185 3 et et FW cord-329245-6tj2k1yn 185 4 al al NNP cord-329245-6tj2k1yn 185 5 . . NNP cord-329245-6tj2k1yn 185 6 , , , cord-329245-6tj2k1yn 185 7 1982 1982 CD cord-329245-6tj2k1yn 185 8 ; ; : cord-329245-6tj2k1yn 185 9 Sturman Sturman NNP cord-329245-6tj2k1yn 185 10 et et NNP cord-329245-6tj2k1yn 185 11 al al NNP cord-329245-6tj2k1yn 185 12 . . NNP cord-329245-6tj2k1yn 185 13 , , , cord-329245-6tj2k1yn 185 14 1980 1980 CD cord-329245-6tj2k1yn 185 15 ) ) -RRB- cord-329245-6tj2k1yn 185 16 . . . cord-329245-6tj2k1yn 186 1 Thus thus RB cord-329245-6tj2k1yn 186 2 the the DT cord-329245-6tj2k1yn 186 3 physical physical JJ cord-329245-6tj2k1yn 186 4 nature nature NN cord-329245-6tj2k1yn 186 5 of of IN cord-329245-6tj2k1yn 186 6 the the DT cord-329245-6tj2k1yn 186 7 coronavirus coronavirus NN cord-329245-6tj2k1yn 186 8 envelope envelope NN cord-329245-6tj2k1yn 186 9 must must MD cord-329245-6tj2k1yn 186 10 be be VB cord-329245-6tj2k1yn 186 11 largely largely RB cord-329245-6tj2k1yn 186 12 determined determine VBN cord-329245-6tj2k1yn 186 13 by by IN cord-329245-6tj2k1yn 186 14 the the DT cord-329245-6tj2k1yn 186 15 characteristics characteristic NNS cord-329245-6tj2k1yn 186 16 of of IN cord-329245-6tj2k1yn 186 17 the the DT cord-329245-6tj2k1yn 186 18 M M NNP cord-329245-6tj2k1yn 186 19 protein protein NN cord-329245-6tj2k1yn 186 20 . . . cord-329245-6tj2k1yn 187 1 Interestingly interestingly RB cord-329245-6tj2k1yn 187 2 , , , cord-329245-6tj2k1yn 187 3 however however RB cord-329245-6tj2k1yn 187 4 , , , cord-329245-6tj2k1yn 187 5 the the DT cord-329245-6tj2k1yn 187 6 M M NNP cord-329245-6tj2k1yn 187 7 protein protein NN cord-329245-6tj2k1yn 187 8 can can MD cord-329245-6tj2k1yn 187 9 not not RB cord-329245-6tj2k1yn 187 10 drive drive VB cord-329245-6tj2k1yn 187 11 coronavirus coronavirus NN cord-329245-6tj2k1yn 187 12 budding bud VBG cord-329245-6tj2k1yn 187 13 by by IN cord-329245-6tj2k1yn 187 14 itself -PRON- PRP cord-329245-6tj2k1yn 187 15 , , , cord-329245-6tj2k1yn 187 16 but but CC cord-329245-6tj2k1yn 187 17 displays display VBZ cord-329245-6tj2k1yn 187 18 a a DT cord-329245-6tj2k1yn 187 19 strict strict JJ cord-329245-6tj2k1yn 187 20 requirement requirement NN cord-329245-6tj2k1yn 187 21 for for IN cord-329245-6tj2k1yn 187 22 a a DT cord-329245-6tj2k1yn 187 23 small small JJ cord-329245-6tj2k1yn 187 24 amount amount NN cord-329245-6tj2k1yn 187 25 of of IN cord-329245-6tj2k1yn 187 26 E e NN cord-329245-6tj2k1yn 187 27 protein protein NN cord-329245-6tj2k1yn 187 28 for for IN cord-329245-6tj2k1yn 187 29 VLP VLP NNP cord-329245-6tj2k1yn 187 30 formation formation NN cord-329245-6tj2k1yn 188 1 ( ( -LRB- cord-329245-6tj2k1yn 188 2 Baudoux Baudoux NNP cord-329245-6tj2k1yn 188 3 et et NNP cord-329245-6tj2k1yn 188 4 al al NNP cord-329245-6tj2k1yn 188 5 . . NNP cord-329245-6tj2k1yn 188 6 , , , cord-329245-6tj2k1yn 188 7 1998 1998 CD cord-329245-6tj2k1yn 188 8 ; ; : cord-329245-6tj2k1yn 189 1 Corse Corse NNP cord-329245-6tj2k1yn 189 2 and and CC cord-329245-6tj2k1yn 189 3 Machamer Machamer NNP cord-329245-6tj2k1yn 189 4 , , , cord-329245-6tj2k1yn 189 5 2000 2000 CD cord-329245-6tj2k1yn 189 6 ; ; : cord-329245-6tj2k1yn 189 7 Godeke Godeke NNP cord-329245-6tj2k1yn 189 8 et et FW cord-329245-6tj2k1yn 189 9 al al NNP cord-329245-6tj2k1yn 189 10 . . NNP cord-329245-6tj2k1yn 189 11 , , , cord-329245-6tj2k1yn 189 12 2000 2000 CD cord-329245-6tj2k1yn 189 13 ; ; : cord-329245-6tj2k1yn 189 14 Vennema Vennema NNP cord-329245-6tj2k1yn 189 15 et et FW cord-329245-6tj2k1yn 189 16 al al NNP cord-329245-6tj2k1yn 189 17 . . NNP cord-329245-6tj2k1yn 189 18 , , , cord-329245-6tj2k1yn 189 19 1996 1996 CD cord-329245-6tj2k1yn 189 20 ) ) -RRB- cord-329245-6tj2k1yn 189 21 . . . cord-329245-6tj2k1yn 190 1 We -PRON- PRP cord-329245-6tj2k1yn 190 2 have have VBP cord-329245-6tj2k1yn 190 3 observed observe VBN cord-329245-6tj2k1yn 190 4 this this DT cord-329245-6tj2k1yn 190 5 low low JJ cord-329245-6tj2k1yn 190 6 E e NN cord-329245-6tj2k1yn 190 7 protein protein NN cord-329245-6tj2k1yn 190 8 : : : cord-329245-6tj2k1yn 190 9 M m NN cord-329245-6tj2k1yn 190 10 protein protein NN cord-329245-6tj2k1yn 190 11 ratio ratio NN cord-329245-6tj2k1yn 190 12 in in IN cord-329245-6tj2k1yn 190 13 IBV IBV NNP cord-329245-6tj2k1yn 190 14 VLPs vlp NNS cord-329245-6tj2k1yn 190 15 ( ( -LRB- cord-329245-6tj2k1yn 190 16 Fig Fig NNP cord-329245-6tj2k1yn 190 17 . . NNP cord-329245-6tj2k1yn 190 18 6 6 CD cord-329245-6tj2k1yn 190 19 , , , cord-329245-6tj2k1yn 190 20 lane lane NN cord-329245-6tj2k1yn 190 21 14 14 CD cord-329245-6tj2k1yn 190 22 ) ) -RRB- cord-329245-6tj2k1yn 190 23 ; ; : cord-329245-6tj2k1yn 190 24 measurements measurement NNS cord-329245-6tj2k1yn 190 25 of of IN cord-329245-6tj2k1yn 190 26 the the DT cord-329245-6tj2k1yn 190 27 amount amount NN cord-329245-6tj2k1yn 190 28 of of IN cord-329245-6tj2k1yn 190 29 E E NNP cord-329245-6tj2k1yn 190 30 and and CC cord-329245-6tj2k1yn 190 31 M M NNP cord-329245-6tj2k1yn 190 32 proteins protein NNS cord-329245-6tj2k1yn 190 33 in in IN cord-329245-6tj2k1yn 190 34 IBV IBV NNP cord-329245-6tj2k1yn 190 35 virions virion NNS cord-329245-6tj2k1yn 190 36 also also RB cord-329245-6tj2k1yn 190 37 reveal reveal VBP cord-329245-6tj2k1yn 190 38 a a DT cord-329245-6tj2k1yn 190 39 relatively relatively RB cord-329245-6tj2k1yn 190 40 low low JJ cord-329245-6tj2k1yn 190 41 amount amount NN cord-329245-6tj2k1yn 190 42 of of IN cord-329245-6tj2k1yn 190 43 E e NN cord-329245-6tj2k1yn 190 44 protein protein NN cord-329245-6tj2k1yn 190 45 ( ( -LRB- cord-329245-6tj2k1yn 190 46 Liu Liu NNP cord-329245-6tj2k1yn 190 47 and and CC cord-329245-6tj2k1yn 190 48 Inglis Inglis NNP cord-329245-6tj2k1yn 190 49 , , , cord-329245-6tj2k1yn 190 50 1991 1991 CD cord-329245-6tj2k1yn 190 51 ) ) -RRB- cord-329245-6tj2k1yn 190 52 . . . cord-329245-6tj2k1yn 191 1 We -PRON- PRP cord-329245-6tj2k1yn 191 2 favor favor VBP cord-329245-6tj2k1yn 191 3 the the DT cord-329245-6tj2k1yn 191 4 hypothesis hypothesis NN cord-329245-6tj2k1yn 191 5 that that IN cord-329245-6tj2k1yn 191 6 VLP VLP NNP cord-329245-6tj2k1yn 191 7 formation formation NN cord-329245-6tj2k1yn 191 8 involves involve VBZ cord-329245-6tj2k1yn 191 9 a a DT cord-329245-6tj2k1yn 191 10 specific specific JJ cord-329245-6tj2k1yn 191 11 membrane membrane NN cord-329245-6tj2k1yn 191 12 curvature curvature NN cord-329245-6tj2k1yn 191 13 that that WDT cord-329245-6tj2k1yn 191 14 is be VBZ cord-329245-6tj2k1yn 191 15 induced induce VBN cord-329245-6tj2k1yn 191 16 by by IN cord-329245-6tj2k1yn 191 17 the the DT cord-329245-6tj2k1yn 191 18 proper proper JJ cord-329245-6tj2k1yn 191 19 ratio ratio NN cord-329245-6tj2k1yn 191 20 of of IN cord-329245-6tj2k1yn 191 21 M M NNP cord-329245-6tj2k1yn 191 22 and and CC cord-329245-6tj2k1yn 191 23 E e NN cord-329245-6tj2k1yn 191 24 proteins protein NNS cord-329245-6tj2k1yn 191 25 . . . cord-329245-6tj2k1yn 192 1 The the DT cord-329245-6tj2k1yn 192 2 mechanism mechanism NN cord-329245-6tj2k1yn 192 3 by by IN cord-329245-6tj2k1yn 192 4 which which WDT cord-329245-6tj2k1yn 192 5 a a DT cord-329245-6tj2k1yn 192 6 small small JJ cord-329245-6tj2k1yn 192 7 amount amount NN cord-329245-6tj2k1yn 192 8 of of IN cord-329245-6tj2k1yn 192 9 E e NN cord-329245-6tj2k1yn 192 10 protein protein NN cord-329245-6tj2k1yn 192 11 interacts interact VBZ cord-329245-6tj2k1yn 192 12 with with IN cord-329245-6tj2k1yn 192 13 a a DT cord-329245-6tj2k1yn 192 14 large large JJ cord-329245-6tj2k1yn 192 15 amount amount NN cord-329245-6tj2k1yn 192 16 of of IN cord-329245-6tj2k1yn 192 17 M M NNP cord-329245-6tj2k1yn 192 18 protein protein NN cord-329245-6tj2k1yn 192 19 to to TO cord-329245-6tj2k1yn 192 20 induce induce VB cord-329245-6tj2k1yn 192 21 invagination invagination NN cord-329245-6tj2k1yn 192 22 and and CC cord-329245-6tj2k1yn 192 23 pinching pinching NN cord-329245-6tj2k1yn 192 24 of of IN cord-329245-6tj2k1yn 192 25 the the DT cord-329245-6tj2k1yn 192 26 membrane membrane NN cord-329245-6tj2k1yn 192 27 to to TO cord-329245-6tj2k1yn 192 28 form form VB cord-329245-6tj2k1yn 192 29 VLPs VLPs NNP cord-329245-6tj2k1yn 192 30 Fig Fig NNP cord-329245-6tj2k1yn 192 31 . . . cord-329245-6tj2k1yn 193 1 7 7 CD cord-329245-6tj2k1yn 193 2 . . . cord-329245-6tj2k1yn 194 1 Cytoplasmic Cytoplasmic NNP cord-329245-6tj2k1yn 194 2 and and CC cord-329245-6tj2k1yn 194 3 transmembrane transmembrane NN cord-329245-6tj2k1yn 194 4 region region NN cord-329245-6tj2k1yn 194 5 deletion deletion NN cord-329245-6tj2k1yn 194 6 mutants mutant NNS cord-329245-6tj2k1yn 194 7 of of IN cord-329245-6tj2k1yn 194 8 IBV IBV NNP cord-329245-6tj2k1yn 194 9 M M NNP cord-329245-6tj2k1yn 194 10 do do VBP cord-329245-6tj2k1yn 194 11 not not RB cord-329245-6tj2k1yn 194 12 support support VB cord-329245-6tj2k1yn 194 13 VLP VLP NNP cord-329245-6tj2k1yn 194 14 formation formation NN cord-329245-6tj2k1yn 194 15 . . . cord-329245-6tj2k1yn 195 1 OST7 OST7 NNP cord-329245-6tj2k1yn 195 2 - - HYPH cord-329245-6tj2k1yn 195 3 1 1 CD cord-329245-6tj2k1yn 195 4 cells cell NNS cord-329245-6tj2k1yn 195 5 expressing express VBG cord-329245-6tj2k1yn 195 6 the the DT cord-329245-6tj2k1yn 195 7 indicated indicate VBN cord-329245-6tj2k1yn 195 8 proteins protein NNS cord-329245-6tj2k1yn 195 9 were be VBD cord-329245-6tj2k1yn 195 10 labeled label VBN cord-329245-6tj2k1yn 195 11 with with IN cord-329245-6tj2k1yn 195 12 with with IN cord-329245-6tj2k1yn 195 13 [ [ -LRB- cord-329245-6tj2k1yn 195 14 35 35 CD cord-329245-6tj2k1yn 195 15 S]methionine S]methionine NNP cord-329245-6tj2k1yn 195 16 - - HYPH cord-329245-6tj2k1yn 195 17 cysteine cysteine NN cord-329245-6tj2k1yn 195 18 for for IN cord-329245-6tj2k1yn 195 19 1 1 CD cord-329245-6tj2k1yn 195 20 h h NN cord-329245-6tj2k1yn 195 21 and and CC cord-329245-6tj2k1yn 195 22 chased chase VBD cord-329245-6tj2k1yn 195 23 for for IN cord-329245-6tj2k1yn 195 24 3 3 CD cord-329245-6tj2k1yn 195 25 h h NN cord-329245-6tj2k1yn 195 26 , , , cord-329245-6tj2k1yn 195 27 and and CC cord-329245-6tj2k1yn 195 28 the the DT cord-329245-6tj2k1yn 195 29 supernatants supernatant NNS cord-329245-6tj2k1yn 195 30 and and CC cord-329245-6tj2k1yn 195 31 cells cell NNS cord-329245-6tj2k1yn 195 32 were be VBD cord-329245-6tj2k1yn 195 33 harvested harvest VBN cord-329245-6tj2k1yn 195 34 and and CC cord-329245-6tj2k1yn 195 35 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 195 36 with with IN cord-329245-6tj2k1yn 195 37 anti anti JJ cord-329245-6tj2k1yn 195 38 - - JJ cord-329245-6tj2k1yn 195 39 E e JJ cord-329245-6tj2k1yn 195 40 and and CC cord-329245-6tj2k1yn 195 41 anti anti JJ cord-329245-6tj2k1yn 195 42 - - JJ cord-329245-6tj2k1yn 195 43 M m JJ cord-329245-6tj2k1yn 195 44 antibodies antibody NNS cord-329245-6tj2k1yn 195 45 as as IN cord-329245-6tj2k1yn 195 46 described describe VBN cord-329245-6tj2k1yn 195 47 under under IN cord-329245-6tj2k1yn 195 48 Materials material NNS cord-329245-6tj2k1yn 195 49 and and CC cord-329245-6tj2k1yn 195 50 methods method NNS cord-329245-6tj2k1yn 195 51 . . . cord-329245-6tj2k1yn 196 1 The the DT cord-329245-6tj2k1yn 196 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 196 3 were be VBD cord-329245-6tj2k1yn 196 4 analyzed analyze VBN cord-329245-6tj2k1yn 196 5 by by IN cord-329245-6tj2k1yn 196 6 SDS-17.5 SDS-17.5 NNP cord-329245-6tj2k1yn 196 7 % % NN cord-329245-6tj2k1yn 196 8 PAGE PAGE NNP cord-329245-6tj2k1yn 196 9 and and CC cord-329245-6tj2k1yn 196 10 fluorography fluorography NN cord-329245-6tj2k1yn 196 11 . . . cord-329245-6tj2k1yn 197 1 The the DT cord-329245-6tj2k1yn 197 2 supernatant supernatant JJ cord-329245-6tj2k1yn 197 3 samples sample NNS cord-329245-6tj2k1yn 197 4 ( ( -LRB- cord-329245-6tj2k1yn 197 5 lanes lane NNS cord-329245-6tj2k1yn 197 6 12 12 CD cord-329245-6tj2k1yn 197 7 - - SYM cord-329245-6tj2k1yn 197 8 22 22 CD cord-329245-6tj2k1yn 197 9 ) ) -RRB- cord-329245-6tj2k1yn 197 10 were be VBD cord-329245-6tj2k1yn 197 11 exposed expose VBN cord-329245-6tj2k1yn 197 12 to to TO cord-329245-6tj2k1yn 197 13 film film VB cord-329245-6tj2k1yn 197 14 13 13 CD cord-329245-6tj2k1yn 197 15 times time NNS cord-329245-6tj2k1yn 197 16 longer long JJR cord-329245-6tj2k1yn 197 17 than than IN cord-329245-6tj2k1yn 197 18 the the DT cord-329245-6tj2k1yn 197 19 cell cell NN cord-329245-6tj2k1yn 197 20 samples sample NNS cord-329245-6tj2k1yn 197 21 ( ( -LRB- cord-329245-6tj2k1yn 197 22 lanes lane NNS cord-329245-6tj2k1yn 197 23 1 1 CD cord-329245-6tj2k1yn 197 24 - - SYM cord-329245-6tj2k1yn 197 25 11 11 CD cord-329245-6tj2k1yn 197 26 ) ) -RRB- cord-329245-6tj2k1yn 197 27 . . . cord-329245-6tj2k1yn 198 1 The the DT cord-329245-6tj2k1yn 198 2 Mct Mct NNP cord-329245-6tj2k1yn 198 3 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 198 4 1 1 CD cord-329245-6tj2k1yn 198 5 and and CC cord-329245-6tj2k1yn 198 6 Mct Mct NNP cord-329245-6tj2k1yn 198 7 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 198 8 2 2 CD cord-329245-6tj2k1yn 198 9 mutants mutant NNS cord-329245-6tj2k1yn 198 10 are be VBP cord-329245-6tj2k1yn 198 11 not not RB cord-329245-6tj2k1yn 198 12 radiolabeled radiolabele VBN cord-329245-6tj2k1yn 198 13 as as RB cord-329245-6tj2k1yn 198 14 efficiently efficiently RB cord-329245-6tj2k1yn 198 15 as as IN cord-329245-6tj2k1yn 198 16 wild wild JJ cord-329245-6tj2k1yn 198 17 - - HYPH cord-329245-6tj2k1yn 198 18 type type NN cord-329245-6tj2k1yn 198 19 M M NNP cord-329245-6tj2k1yn 198 20 and and CC cord-329245-6tj2k1yn 198 21 the the DT cord-329245-6tj2k1yn 198 22 Mct Mct NNP cord-329245-6tj2k1yn 198 23 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 198 24 3 3 CD cord-329245-6tj2k1yn 198 25 and and CC cord-329245-6tj2k1yn 198 26 M M NNP cord-329245-6tj2k1yn 198 27 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 198 28 m2,3 m2,3 NNP cord-329245-6tj2k1yn 198 29 mutants mutant NNS cord-329245-6tj2k1yn 198 30 because because IN cord-329245-6tj2k1yn 198 31 they -PRON- PRP cord-329245-6tj2k1yn 198 32 have have VBP cord-329245-6tj2k1yn 198 33 fewer few JJR cord-329245-6tj2k1yn 198 34 methionines methionine NNS cord-329245-6tj2k1yn 198 35 and and CC cord-329245-6tj2k1yn 198 36 cysteines cysteine NNS cord-329245-6tj2k1yn 198 37 due due IN cord-329245-6tj2k1yn 198 38 to to IN cord-329245-6tj2k1yn 198 39 their -PRON- PRP$ cord-329245-6tj2k1yn 198 40 deletions deletion NNS cord-329245-6tj2k1yn 198 41 . . . cord-329245-6tj2k1yn 199 1 These these DT cord-329245-6tj2k1yn 199 2 data datum NNS cord-329245-6tj2k1yn 199 3 are be VBP cord-329245-6tj2k1yn 199 4 representative representative JJ cord-329245-6tj2k1yn 199 5 of of IN cord-329245-6tj2k1yn 199 6 at at RB cord-329245-6tj2k1yn 199 7 least least RBS cord-329245-6tj2k1yn 199 8 three three CD cord-329245-6tj2k1yn 199 9 independent independent JJ cord-329245-6tj2k1yn 199 10 experiments experiment NNS cord-329245-6tj2k1yn 199 11 . . . cord-329245-6tj2k1yn 200 1 remains remain VBZ cord-329245-6tj2k1yn 200 2 unclear unclear JJ cord-329245-6tj2k1yn 200 3 , , , cord-329245-6tj2k1yn 200 4 but but CC cord-329245-6tj2k1yn 200 5 its -PRON- PRP$ cord-329245-6tj2k1yn 200 6 elucidation elucidation NN cord-329245-6tj2k1yn 200 7 is be VBZ cord-329245-6tj2k1yn 200 8 likely likely JJ cord-329245-6tj2k1yn 200 9 to to TO cord-329245-6tj2k1yn 200 10 be be VB cord-329245-6tj2k1yn 200 11 essential essential JJ cord-329245-6tj2k1yn 200 12 for for IN cord-329245-6tj2k1yn 200 13 a a DT cord-329245-6tj2k1yn 200 14 complete complete JJ cord-329245-6tj2k1yn 200 15 understanding understanding NN cord-329245-6tj2k1yn 200 16 of of IN cord-329245-6tj2k1yn 200 17 coronavirus coronavirus NN cord-329245-6tj2k1yn 200 18 assembly assembly NN cord-329245-6tj2k1yn 200 19 . . . cord-329245-6tj2k1yn 201 1 Several several JJ cord-329245-6tj2k1yn 201 2 studies study NNS cord-329245-6tj2k1yn 201 3 have have VBP cord-329245-6tj2k1yn 201 4 addressed address VBN cord-329245-6tj2k1yn 201 5 the the DT cord-329245-6tj2k1yn 201 6 question question NN cord-329245-6tj2k1yn 201 7 of of IN cord-329245-6tj2k1yn 201 8 how how WRB cord-329245-6tj2k1yn 201 9 the the DT cord-329245-6tj2k1yn 201 10 coronavirus coronavirus NN cord-329245-6tj2k1yn 201 11 E e NN cord-329245-6tj2k1yn 201 12 and and CC cord-329245-6tj2k1yn 201 13 M M NNP cord-329245-6tj2k1yn 201 14 proteins protein NNS cord-329245-6tj2k1yn 201 15 interact interact VBP cord-329245-6tj2k1yn 201 16 in in IN cord-329245-6tj2k1yn 201 17 VLP VLP NNP cord-329245-6tj2k1yn 201 18 formation formation NN cord-329245-6tj2k1yn 201 19 . . . cord-329245-6tj2k1yn 202 1 Rottier Rottier NNP cord-329245-6tj2k1yn 202 2 and and CC cord-329245-6tj2k1yn 202 3 colleagues colleague NNS cord-329245-6tj2k1yn 202 4 ( ( -LRB- cord-329245-6tj2k1yn 202 5 De De NNP cord-329245-6tj2k1yn 202 6 Haan Haan NNP cord-329245-6tj2k1yn 202 7 et et FW cord-329245-6tj2k1yn 202 8 al al NNP cord-329245-6tj2k1yn 202 9 . . NNP cord-329245-6tj2k1yn 202 10 , , , cord-329245-6tj2k1yn 202 11 1998 1998 CD cord-329245-6tj2k1yn 202 12 ) ) -RRB- cord-329245-6tj2k1yn 202 13 studied study VBD cord-329245-6tj2k1yn 202 14 the the DT cord-329245-6tj2k1yn 202 15 primary primary JJ cord-329245-6tj2k1yn 202 16 sequence sequence NN cord-329245-6tj2k1yn 202 17 requirements requirement NNS cord-329245-6tj2k1yn 202 18 for for IN cord-329245-6tj2k1yn 202 19 incorporation incorporation NN cord-329245-6tj2k1yn 202 20 of of IN cord-329245-6tj2k1yn 202 21 the the DT cord-329245-6tj2k1yn 202 22 MHV MHV NNP cord-329245-6tj2k1yn 202 23 M M NNP cord-329245-6tj2k1yn 202 24 protein protein NN cord-329245-6tj2k1yn 202 25 into into IN cord-329245-6tj2k1yn 202 26 VLPs vlp NNS cord-329245-6tj2k1yn 202 27 . . . cord-329245-6tj2k1yn 203 1 They -PRON- PRP cord-329245-6tj2k1yn 203 2 found find VBD cord-329245-6tj2k1yn 203 3 that that IN cord-329245-6tj2k1yn 203 4 all all DT cord-329245-6tj2k1yn 203 5 regions region NNS cord-329245-6tj2k1yn 203 6 of of IN cord-329245-6tj2k1yn 203 7 MHV MHV NNP cord-329245-6tj2k1yn 203 8 M M NNP cord-329245-6tj2k1yn 203 9 seem seem VBP cord-329245-6tj2k1yn 203 10 to to TO cord-329245-6tj2k1yn 203 11 be be VB cord-329245-6tj2k1yn 203 12 important important JJ cord-329245-6tj2k1yn 203 13 for for IN cord-329245-6tj2k1yn 203 14 VLP VLP NNP cord-329245-6tj2k1yn 203 15 formation formation NN cord-329245-6tj2k1yn 203 16 , , , cord-329245-6tj2k1yn 203 17 since since IN cord-329245-6tj2k1yn 203 18 mutations mutation NNS cord-329245-6tj2k1yn 203 19 in in IN cord-329245-6tj2k1yn 203 20 the the DT cord-329245-6tj2k1yn 203 21 short short JJ cord-329245-6tj2k1yn 203 22 lumenal lumenal JJ cord-329245-6tj2k1yn 203 23 domain domain NN cord-329245-6tj2k1yn 203 24 , , , cord-329245-6tj2k1yn 203 25 the the DT cord-329245-6tj2k1yn 203 26 transmembrane transmembrane NN cord-329245-6tj2k1yn 203 27 region region NN cord-329245-6tj2k1yn 203 28 , , , cord-329245-6tj2k1yn 203 29 and and CC cord-329245-6tj2k1yn 203 30 the the DT cord-329245-6tj2k1yn 203 31 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 203 32 tail tail NN cord-329245-6tj2k1yn 203 33 all all DT cord-329245-6tj2k1yn 203 34 impaired impair VBN cord-329245-6tj2k1yn 203 35 VLP VLP NNP cord-329245-6tj2k1yn 203 36 formation formation NN cord-329245-6tj2k1yn 203 37 to to IN cord-329245-6tj2k1yn 203 38 some some DT cord-329245-6tj2k1yn 203 39 extent extent NN cord-329245-6tj2k1yn 203 40 . . . cord-329245-6tj2k1yn 204 1 These these DT cord-329245-6tj2k1yn 204 2 results result NNS cord-329245-6tj2k1yn 204 3 are be VBP cord-329245-6tj2k1yn 204 4 consistent consistent JJ cord-329245-6tj2k1yn 204 5 with with IN cord-329245-6tj2k1yn 204 6 our -PRON- PRP$ cord-329245-6tj2k1yn 204 7 observation observation NN cord-329245-6tj2k1yn 204 8 that that IN cord-329245-6tj2k1yn 204 9 deletions deletion NNS cord-329245-6tj2k1yn 204 10 in in IN cord-329245-6tj2k1yn 204 11 both both CC cord-329245-6tj2k1yn 204 12 the the DT cord-329245-6tj2k1yn 204 13 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 204 14 and and CC cord-329245-6tj2k1yn 204 15 the the DT cord-329245-6tj2k1yn 204 16 transmembrane transmembrane NN cord-329245-6tj2k1yn 204 17 domains domain NNS cord-329245-6tj2k1yn 204 18 of of IN cord-329245-6tj2k1yn 204 19 IBV IBV NNP cord-329245-6tj2k1yn 204 20 M M NNP cord-329245-6tj2k1yn 204 21 prevent prevent VBP cord-329245-6tj2k1yn 204 22 VLP VLP NNP cord-329245-6tj2k1yn 204 23 formation formation NN cord-329245-6tj2k1yn 204 24 . . . cord-329245-6tj2k1yn 205 1 Interestingly interestingly RB cord-329245-6tj2k1yn 205 2 , , , cord-329245-6tj2k1yn 205 3 the the DT cord-329245-6tj2k1yn 205 4 extreme extreme JJ cord-329245-6tj2k1yn 205 5 C c NN cord-329245-6tj2k1yn 205 6 - - HYPH cord-329245-6tj2k1yn 205 7 terminus terminus NN cord-329245-6tj2k1yn 205 8 of of IN cord-329245-6tj2k1yn 205 9 the the DT cord-329245-6tj2k1yn 205 10 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 205 11 tail tail NN cord-329245-6tj2k1yn 205 12 MHV MHV NNP cord-329245-6tj2k1yn 205 13 M M NNP cord-329245-6tj2k1yn 205 14 was be VBD cord-329245-6tj2k1yn 205 15 found find VBN cord-329245-6tj2k1yn 205 16 to to TO cord-329245-6tj2k1yn 205 17 be be VB cord-329245-6tj2k1yn 205 18 especially especially RB cord-329245-6tj2k1yn 205 19 important important JJ cord-329245-6tj2k1yn 205 20 , , , cord-329245-6tj2k1yn 205 21 since since IN cord-329245-6tj2k1yn 205 22 deletion deletion NN cord-329245-6tj2k1yn 205 23 of of IN cord-329245-6tj2k1yn 205 24 only only RB cord-329245-6tj2k1yn 205 25 the the DT cord-329245-6tj2k1yn 205 26 C C NNP cord-329245-6tj2k1yn 205 27 - - HYPH cord-329245-6tj2k1yn 205 28 terminal terminal JJ cord-329245-6tj2k1yn 205 29 threonine threonine NN cord-329245-6tj2k1yn 205 30 residue residue NN cord-329245-6tj2k1yn 205 31 prevented prevent VBD cord-329245-6tj2k1yn 205 32 VLP VLP NNP cord-329245-6tj2k1yn 205 33 formation formation NN cord-329245-6tj2k1yn 205 34 ( ( -LRB- cord-329245-6tj2k1yn 205 35 De De NNP cord-329245-6tj2k1yn 205 36 Haan Haan NNP cord-329245-6tj2k1yn 205 37 et et FW cord-329245-6tj2k1yn 205 38 al al NNP cord-329245-6tj2k1yn 205 39 . . NNP cord-329245-6tj2k1yn 205 40 , , , cord-329245-6tj2k1yn 205 41 1998 1998 CD cord-329245-6tj2k1yn 205 42 ) ) -RRB- cord-329245-6tj2k1yn 205 43 . . . cord-329245-6tj2k1yn 206 1 We -PRON- PRP cord-329245-6tj2k1yn 206 2 were be VBD cord-329245-6tj2k1yn 206 3 unable unable JJ cord-329245-6tj2k1yn 206 4 to to TO cord-329245-6tj2k1yn 206 5 examine examine VB cord-329245-6tj2k1yn 206 6 the the DT cord-329245-6tj2k1yn 206 7 role role NN cord-329245-6tj2k1yn 206 8 of of IN cord-329245-6tj2k1yn 206 9 the the DT cord-329245-6tj2k1yn 206 10 extreme extreme JJ cord-329245-6tj2k1yn 206 11 C c NN cord-329245-6tj2k1yn 206 12 - - HYPH cord-329245-6tj2k1yn 206 13 terminus terminus NN cord-329245-6tj2k1yn 206 14 of of IN cord-329245-6tj2k1yn 206 15 the the DT cord-329245-6tj2k1yn 206 16 IBV IBV NNP cord-329245-6tj2k1yn 206 17 M M NNP cord-329245-6tj2k1yn 206 18 protein protein NN cord-329245-6tj2k1yn 206 19 in in IN cord-329245-6tj2k1yn 206 20 VLP VLP NNP cord-329245-6tj2k1yn 206 21 formation formation NN cord-329245-6tj2k1yn 206 22 because because IN cord-329245-6tj2k1yn 206 23 our -PRON- PRP$ cord-329245-6tj2k1yn 206 24 antibody antibody NN cord-329245-6tj2k1yn 206 25 to to IN cord-329245-6tj2k1yn 206 26 IBV IBV NNP cord-329245-6tj2k1yn 206 27 M M NNP cord-329245-6tj2k1yn 206 28 recognizes recognize VBZ cord-329245-6tj2k1yn 206 29 this this DT cord-329245-6tj2k1yn 206 30 sequence sequence NN cord-329245-6tj2k1yn 206 31 . . . cord-329245-6tj2k1yn 207 1 Laude laude NN cord-329245-6tj2k1yn 207 2 and and CC cord-329245-6tj2k1yn 207 3 colleagues colleague NNS cord-329245-6tj2k1yn 207 4 ( ( -LRB- cord-329245-6tj2k1yn 207 5 Baudoux Baudoux NNP cord-329245-6tj2k1yn 207 6 et et NNP cord-329245-6tj2k1yn 207 7 al al NNP cord-329245-6tj2k1yn 207 8 . . NNP cord-329245-6tj2k1yn 207 9 , , , cord-329245-6tj2k1yn 207 10 1998 1998 CD cord-329245-6tj2k1yn 207 11 ) ) -RRB- cord-329245-6tj2k1yn 207 12 showed show VBD cord-329245-6tj2k1yn 207 13 that that IN cord-329245-6tj2k1yn 207 14 coexpression coexpression NN cord-329245-6tj2k1yn 207 15 of of IN cord-329245-6tj2k1yn 207 16 the the DT cord-329245-6tj2k1yn 207 17 E E NNP cord-329245-6tj2k1yn 207 18 and and CC cord-329245-6tj2k1yn 207 19 M M NNP cord-329245-6tj2k1yn 207 20 proteins protein NNS cord-329245-6tj2k1yn 207 21 of of IN cord-329245-6tj2k1yn 207 22 transmissible transmissible JJ cord-329245-6tj2k1yn 207 23 gastroenteritis gastroenteritis NN cord-329245-6tj2k1yn 207 24 virus virus NN cord-329245-6tj2k1yn 207 25 ( ( -LRB- cord-329245-6tj2k1yn 207 26 TGEV TGEV NNP cord-329245-6tj2k1yn 207 27 ) ) -RRB- cord-329245-6tj2k1yn 207 28 and and CC cord-329245-6tj2k1yn 207 29 of of IN cord-329245-6tj2k1yn 207 30 bovine bovine JJ cord-329245-6tj2k1yn 207 31 coronavirus coronavirus NN cord-329245-6tj2k1yn 207 32 ( ( -LRB- cord-329245-6tj2k1yn 207 33 BCV BCV NNP cord-329245-6tj2k1yn 207 34 ) ) -RRB- cord-329245-6tj2k1yn 207 35 resulted result VBD cord-329245-6tj2k1yn 207 36 in in IN cord-329245-6tj2k1yn 207 37 VLP VLP NNP cord-329245-6tj2k1yn 207 38 production production NN cord-329245-6tj2k1yn 207 39 . . . cord-329245-6tj2k1yn 208 1 In in IN cord-329245-6tj2k1yn 208 2 this this DT cord-329245-6tj2k1yn 208 3 study study NN cord-329245-6tj2k1yn 208 4 VLPs vlp NNS cord-329245-6tj2k1yn 208 5 were be VBD cord-329245-6tj2k1yn 208 6 also also RB cord-329245-6tj2k1yn 208 7 produced produce VBN cord-329245-6tj2k1yn 208 8 with with IN cord-329245-6tj2k1yn 208 9 a a DT cord-329245-6tj2k1yn 208 10 BCV BCV NNP cord-329245-6tj2k1yn 208 11 - - HYPH cord-329245-6tj2k1yn 208 12 TGEV TGEV NNP cord-329245-6tj2k1yn 208 13 chimeric chimeric JJ cord-329245-6tj2k1yn 208 14 M M NNP cord-329245-6tj2k1yn 208 15 protein protein NN cord-329245-6tj2k1yn 208 16 . . . cord-329245-6tj2k1yn 209 1 Interestingly interestingly RB cord-329245-6tj2k1yn 209 2 , , , cord-329245-6tj2k1yn 209 3 the the DT cord-329245-6tj2k1yn 209 4 chimeric chimeric JJ cord-329245-6tj2k1yn 209 5 M M NNP cord-329245-6tj2k1yn 209 6 protein protein NN cord-329245-6tj2k1yn 209 7 was be VBD cord-329245-6tj2k1yn 209 8 able able JJ cord-329245-6tj2k1yn 209 9 to to TO cord-329245-6tj2k1yn 209 10 form form VB cord-329245-6tj2k1yn 209 11 VLPs vlp NNS cord-329245-6tj2k1yn 209 12 with with IN cord-329245-6tj2k1yn 209 13 TGEV TGEV NNP cord-329245-6tj2k1yn 209 14 E E NNP cord-329245-6tj2k1yn 209 15 , , , cord-329245-6tj2k1yn 209 16 BCV BCV NNP cord-329245-6tj2k1yn 209 17 E E NNP cord-329245-6tj2k1yn 209 18 , , , cord-329245-6tj2k1yn 209 19 and and CC cord-329245-6tj2k1yn 209 20 BCV BCV NNP cord-329245-6tj2k1yn 209 21 - - HYPH cord-329245-6tj2k1yn 209 22 TGEV TGEV NNP cord-329245-6tj2k1yn 209 23 chimeric chimeric JJ cord-329245-6tj2k1yn 209 24 E e NN cord-329245-6tj2k1yn 209 25 proteins protein NNS cord-329245-6tj2k1yn 209 26 . . . cord-329245-6tj2k1yn 210 1 Since since IN cord-329245-6tj2k1yn 210 2 TGEV TGEV NNP cord-329245-6tj2k1yn 210 3 and and CC cord-329245-6tj2k1yn 210 4 BCV BCV NNP cord-329245-6tj2k1yn 210 5 are be VBP cord-329245-6tj2k1yn 210 6 in in IN cord-329245-6tj2k1yn 210 7 different different JJ cord-329245-6tj2k1yn 210 8 coronavirus coronavirus NN cord-329245-6tj2k1yn 210 9 groups group NNS cord-329245-6tj2k1yn 210 10 based base VBN cord-329245-6tj2k1yn 210 11 on on IN cord-329245-6tj2k1yn 210 12 serological serological JJ cord-329245-6tj2k1yn 210 13 and and CC cord-329245-6tj2k1yn 210 14 genomic genomic JJ cord-329245-6tj2k1yn 210 15 sequence sequence NN cord-329245-6tj2k1yn 210 16 relationships relationship NNS cord-329245-6tj2k1yn 210 17 , , , cord-329245-6tj2k1yn 210 18 this this DT cord-329245-6tj2k1yn 210 19 suggests suggest VBZ cord-329245-6tj2k1yn 210 20 that that IN cord-329245-6tj2k1yn 210 21 VLP VLP NNP cord-329245-6tj2k1yn 210 22 formation formation NN cord-329245-6tj2k1yn 210 23 does do VBZ cord-329245-6tj2k1yn 210 24 not not RB cord-329245-6tj2k1yn 210 25 depend depend VB cord-329245-6tj2k1yn 210 26 on on IN cord-329245-6tj2k1yn 210 27 interactions interaction NNS cord-329245-6tj2k1yn 210 28 between between IN cord-329245-6tj2k1yn 210 29 E E NNP cord-329245-6tj2k1yn 210 30 and and CC cord-329245-6tj2k1yn 210 31 M M NNP cord-329245-6tj2k1yn 210 32 proteins protein NNS cord-329245-6tj2k1yn 210 33 that that WDT cord-329245-6tj2k1yn 210 34 are be VBP cord-329245-6tj2k1yn 210 35 based base VBN cord-329245-6tj2k1yn 210 36 on on IN cord-329245-6tj2k1yn 210 37 strict strict JJ cord-329245-6tj2k1yn 210 38 sequence sequence NN cord-329245-6tj2k1yn 210 39 requirements requirement NNS cord-329245-6tj2k1yn 210 40 . . . cord-329245-6tj2k1yn 211 1 Well well RB cord-329245-6tj2k1yn 211 2 - - HYPH cord-329245-6tj2k1yn 211 3 conserved conserve VBN cord-329245-6tj2k1yn 211 4 three three CD cord-329245-6tj2k1yn 211 5 - - HYPH cord-329245-6tj2k1yn 211 6 dimensional dimensional JJ cord-329245-6tj2k1yn 211 7 characteristics characteristic NNS cord-329245-6tj2k1yn 211 8 of of IN cord-329245-6tj2k1yn 211 9 the the DT cord-329245-6tj2k1yn 211 10 E E NNP cord-329245-6tj2k1yn 211 11 and and CC cord-329245-6tj2k1yn 211 12 M M NNP cord-329245-6tj2k1yn 211 13 proteins protein NNS cord-329245-6tj2k1yn 211 14 may may MD cord-329245-6tj2k1yn 211 15 be be VB cord-329245-6tj2k1yn 211 16 instrumental instrumental JJ cord-329245-6tj2k1yn 211 17 in in IN cord-329245-6tj2k1yn 211 18 VLP VLP NNP cord-329245-6tj2k1yn 211 19 formation formation NN cord-329245-6tj2k1yn 211 20 . . . cord-329245-6tj2k1yn 212 1 Our -PRON- PRP$ cord-329245-6tj2k1yn 212 2 results result NNS cord-329245-6tj2k1yn 212 3 suggest suggest VBP cord-329245-6tj2k1yn 212 4 that that IN cord-329245-6tj2k1yn 212 5 VLP VLP NNP cord-329245-6tj2k1yn 212 6 formation formation NN cord-329245-6tj2k1yn 212 7 involves involve VBZ cord-329245-6tj2k1yn 212 8 more more JJR cord-329245-6tj2k1yn 212 9 than than IN cord-329245-6tj2k1yn 212 10 an an DT cord-329245-6tj2k1yn 212 11 interaction interaction NN cord-329245-6tj2k1yn 212 12 between between IN cord-329245-6tj2k1yn 212 13 E E NNP cord-329245-6tj2k1yn 212 14 and and CC cord-329245-6tj2k1yn 212 15 M M NNP cord-329245-6tj2k1yn 212 16 proteins protein NNS cord-329245-6tj2k1yn 212 17 , , , cord-329245-6tj2k1yn 212 18 since since IN cord-329245-6tj2k1yn 212 19 M m NN cord-329245-6tj2k1yn 212 20 protein protein NN cord-329245-6tj2k1yn 212 21 mutants mutant NNS cord-329245-6tj2k1yn 212 22 that that WDT cord-329245-6tj2k1yn 212 23 could could MD cord-329245-6tj2k1yn 212 24 be be VB cord-329245-6tj2k1yn 212 25 crosslinked crosslinke VBN cord-329245-6tj2k1yn 212 26 to to IN cord-329245-6tj2k1yn 212 27 E e NN cord-329245-6tj2k1yn 212 28 protein protein NN cord-329245-6tj2k1yn 212 29 ( ( -LRB- cord-329245-6tj2k1yn 212 30 Mct Mct NNP cord-329245-6tj2k1yn 212 31 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 212 32 3 3 CD cord-329245-6tj2k1yn 212 33 and and CC cord-329245-6tj2k1yn 212 34 M M NNP cord-329245-6tj2k1yn 212 35 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 212 36 m2,3 m2,3 NNP cord-329245-6tj2k1yn 212 37 ; ; : cord-329245-6tj2k1yn 212 38 see see VB cord-329245-6tj2k1yn 212 39 Fig Fig NNP cord-329245-6tj2k1yn 212 40 . . . cord-329245-6tj2k1yn 213 1 5 5 LS cord-329245-6tj2k1yn 213 2 ) ) -RRB- cord-329245-6tj2k1yn 213 3 are be VBP cord-329245-6tj2k1yn 213 4 not not RB cord-329245-6tj2k1yn 213 5 incorporated incorporate VBN cord-329245-6tj2k1yn 213 6 into into IN cord-329245-6tj2k1yn 213 7 virus virus NN cord-329245-6tj2k1yn 213 8 - - HYPH cord-329245-6tj2k1yn 213 9 like like JJ cord-329245-6tj2k1yn 213 10 particles particle NNS cord-329245-6tj2k1yn 213 11 . . . cord-329245-6tj2k1yn 214 1 It -PRON- PRP cord-329245-6tj2k1yn 214 2 is be VBZ cord-329245-6tj2k1yn 214 3 possible possible JJ cord-329245-6tj2k1yn 214 4 that that IN cord-329245-6tj2k1yn 214 5 VLP VLP NNP cord-329245-6tj2k1yn 214 6 formation formation NN cord-329245-6tj2k1yn 214 7 requires require VBZ cord-329245-6tj2k1yn 214 8 interactions interaction NNS cord-329245-6tj2k1yn 214 9 between between IN cord-329245-6tj2k1yn 214 10 M M NNP cord-329245-6tj2k1yn 214 11 proteins protein NNS cord-329245-6tj2k1yn 214 12 , , , cord-329245-6tj2k1yn 214 13 as as IN cord-329245-6tj2k1yn 214 14 proposed propose VBN cord-329245-6tj2k1yn 214 15 by by IN cord-329245-6tj2k1yn 214 16 Rottier Rottier NNP cord-329245-6tj2k1yn 214 17 and and CC cord-329245-6tj2k1yn 214 18 colleagues colleague NNS cord-329245-6tj2k1yn 214 19 . . . cord-329245-6tj2k1yn 215 1 These these DT cord-329245-6tj2k1yn 215 2 workers worker NNS cord-329245-6tj2k1yn 215 3 demonstrated demonstrate VBD cord-329245-6tj2k1yn 215 4 homotypic homotypic JJ cord-329245-6tj2k1yn 215 5 interactions interaction NNS cord-329245-6tj2k1yn 215 6 among among IN cord-329245-6tj2k1yn 215 7 MHV MHV NNP cord-329245-6tj2k1yn 215 8 M M NNP cord-329245-6tj2k1yn 215 9 protein protein NN cord-329245-6tj2k1yn 215 10 molecules molecule NNS cord-329245-6tj2k1yn 215 11 by by IN cord-329245-6tj2k1yn 215 12 coimmunoprecipitation coimmunoprecipitation NN cord-329245-6tj2k1yn 215 13 and and CC cord-329245-6tj2k1yn 215 14 incorporation incorporation NN cord-329245-6tj2k1yn 215 15 of of IN cord-329245-6tj2k1yn 215 16 VLP VLP NNP cord-329245-6tj2k1yn 215 17 assembly assembly NN cord-329245-6tj2k1yn 215 18 - - HYPH cord-329245-6tj2k1yn 215 19 incompetent incompetent JJ cord-329245-6tj2k1yn 215 20 MHV MHV NNP cord-329245-6tj2k1yn 215 21 M m CD cord-329245-6tj2k1yn 215 22 mutants mutant NNS cord-329245-6tj2k1yn 215 23 into into IN cord-329245-6tj2k1yn 215 24 VLPs vlp NNS cord-329245-6tj2k1yn 215 25 via via IN cord-329245-6tj2k1yn 215 26 their -PRON- PRP$ cord-329245-6tj2k1yn 215 27 interaction interaction NN cord-329245-6tj2k1yn 215 28 with with IN cord-329245-6tj2k1yn 215 29 assembly assembly NN cord-329245-6tj2k1yn 215 30 - - HYPH cord-329245-6tj2k1yn 215 31 competent competent JJ cord-329245-6tj2k1yn 215 32 MHV MHV NNP cord-329245-6tj2k1yn 215 33 M M NNP cord-329245-6tj2k1yn 215 34 proteins protein NNS cord-329245-6tj2k1yn 215 35 . . . cord-329245-6tj2k1yn 216 1 These these DT cord-329245-6tj2k1yn 216 2 authors author NNS cord-329245-6tj2k1yn 216 3 hypothesize hypothesize VBP cord-329245-6tj2k1yn 216 4 that that IN cord-329245-6tj2k1yn 216 5 the the DT cord-329245-6tj2k1yn 216 6 VLP VLP NNP cord-329245-6tj2k1yn 216 7 ( ( -LRB- cord-329245-6tj2k1yn 216 8 and and CC cord-329245-6tj2k1yn 216 9 virion virion NN cord-329245-6tj2k1yn 216 10 ) ) -RRB- cord-329245-6tj2k1yn 216 11 envelope envelope NN cord-329245-6tj2k1yn 216 12 mainly mainly RB cord-329245-6tj2k1yn 216 13 consists consist VBZ cord-329245-6tj2k1yn 216 14 of of IN cord-329245-6tj2k1yn 216 15 an an DT cord-329245-6tj2k1yn 216 16 M M NNP cord-329245-6tj2k1yn 216 17 protein protein NN cord-329245-6tj2k1yn 216 18 lattice lattice NN cord-329245-6tj2k1yn 216 19 , , , cord-329245-6tj2k1yn 216 20 with with IN cord-329245-6tj2k1yn 216 21 a a DT cord-329245-6tj2k1yn 216 22 few few JJ cord-329245-6tj2k1yn 216 23 E e NN cord-329245-6tj2k1yn 216 24 proteins protein NNS cord-329245-6tj2k1yn 216 25 interspersed intersperse VBN cord-329245-6tj2k1yn 216 26 within within IN cord-329245-6tj2k1yn 216 27 this this DT cord-329245-6tj2k1yn 216 28 lattice lattice NN cord-329245-6tj2k1yn 216 29 . . . cord-329245-6tj2k1yn 217 1 Although although IN cord-329245-6tj2k1yn 217 2 we -PRON- PRP cord-329245-6tj2k1yn 217 3 have have VBP cord-329245-6tj2k1yn 217 4 not not RB cord-329245-6tj2k1yn 217 5 examined examine VBN cord-329245-6tj2k1yn 217 6 whether whether IN cord-329245-6tj2k1yn 217 7 homotypic homotypic JJ cord-329245-6tj2k1yn 217 8 interactions interaction NNS cord-329245-6tj2k1yn 217 9 between between IN cord-329245-6tj2k1yn 217 10 IBV IBV NNP cord-329245-6tj2k1yn 217 11 M M NNP cord-329245-6tj2k1yn 217 12 proteins protein NNS cord-329245-6tj2k1yn 217 13 exist exist VBP cord-329245-6tj2k1yn 217 14 , , , cord-329245-6tj2k1yn 217 15 we -PRON- PRP cord-329245-6tj2k1yn 217 16 think think VBP cord-329245-6tj2k1yn 217 17 it -PRON- PRP cord-329245-6tj2k1yn 217 18 possible possible JJ cord-329245-6tj2k1yn 217 19 that that IN cord-329245-6tj2k1yn 217 20 such such JJ cord-329245-6tj2k1yn 217 21 interactions interaction NNS cord-329245-6tj2k1yn 217 22 may may MD cord-329245-6tj2k1yn 217 23 be be VB cord-329245-6tj2k1yn 217 24 important important JJ cord-329245-6tj2k1yn 217 25 in in IN cord-329245-6tj2k1yn 217 26 IBV IBV NNP cord-329245-6tj2k1yn 217 27 VLP VLP NNP cord-329245-6tj2k1yn 217 28 assembly assembly NN cord-329245-6tj2k1yn 217 29 . . . cord-329245-6tj2k1yn 218 1 We -PRON- PRP cord-329245-6tj2k1yn 218 2 did do VBD cord-329245-6tj2k1yn 218 3 observe observe VB cord-329245-6tj2k1yn 218 4 a a DT cord-329245-6tj2k1yn 218 5 small small JJ cord-329245-6tj2k1yn 218 6 amount amount NN cord-329245-6tj2k1yn 218 7 of of IN cord-329245-6tj2k1yn 218 8 SDS sds NN cord-329245-6tj2k1yn 218 9 - - HYPH cord-329245-6tj2k1yn 218 10 resistant resistant JJ cord-329245-6tj2k1yn 218 11 IBV IBV NNP cord-329245-6tj2k1yn 218 12 M M NNP cord-329245-6tj2k1yn 218 13 protein protein NN cord-329245-6tj2k1yn 218 14 dimers dimer NNS cord-329245-6tj2k1yn 218 15 in in IN cord-329245-6tj2k1yn 218 16 our -PRON- PRP$ cord-329245-6tj2k1yn 218 17 anti anti JJ cord-329245-6tj2k1yn 218 18 - - JJ cord-329245-6tj2k1yn 218 19 M M NNP cord-329245-6tj2k1yn 218 20 immunoprecipitations immunoprecipitation NNS cord-329245-6tj2k1yn 218 21 ( ( -LRB- cord-329245-6tj2k1yn 218 22 indicated indicate VBN cord-329245-6tj2k1yn 218 23 by by IN cord-329245-6tj2k1yn 218 24 asterisks asterisk NNS cord-329245-6tj2k1yn 218 25 in in IN cord-329245-6tj2k1yn 218 26 Figs Figs NNPS cord-329245-6tj2k1yn 218 27 . . . cord-329245-6tj2k1yn 219 1 2 2 LS cord-329245-6tj2k1yn 219 2 , , , cord-329245-6tj2k1yn 219 3 4 4 CD cord-329245-6tj2k1yn 219 4 , , , cord-329245-6tj2k1yn 219 5 5 5 CD cord-329245-6tj2k1yn 219 6 , , , cord-329245-6tj2k1yn 219 7 and and CC cord-329245-6tj2k1yn 219 8 6 6 CD cord-329245-6tj2k1yn 219 9 ) ) -RRB- cord-329245-6tj2k1yn 219 10 , , , cord-329245-6tj2k1yn 219 11 although although IN cord-329245-6tj2k1yn 219 12 we -PRON- PRP cord-329245-6tj2k1yn 219 13 have have VBP cord-329245-6tj2k1yn 219 14 no no DT cord-329245-6tj2k1yn 219 15 direct direct JJ cord-329245-6tj2k1yn 219 16 evidence evidence NN cord-329245-6tj2k1yn 219 17 that that IN cord-329245-6tj2k1yn 219 18 these these DT cord-329245-6tj2k1yn 219 19 dimers dimer NNS cord-329245-6tj2k1yn 219 20 are be VBP cord-329245-6tj2k1yn 219 21 physiologically physiologically RB cord-329245-6tj2k1yn 219 22 relevant relevant JJ cord-329245-6tj2k1yn 219 23 . . . cord-329245-6tj2k1yn 220 1 We -PRON- PRP cord-329245-6tj2k1yn 220 2 also also RB cord-329245-6tj2k1yn 220 3 showed show VBD cord-329245-6tj2k1yn 220 4 that that IN cord-329245-6tj2k1yn 220 5 the the DT cord-329245-6tj2k1yn 220 6 GEt GEt NNS cord-329245-6tj2k1yn 220 7 chimera chimera NN cord-329245-6tj2k1yn 220 8 could could MD cord-329245-6tj2k1yn 220 9 be be VB cord-329245-6tj2k1yn 220 10 crosslinked crosslinke VBN cord-329245-6tj2k1yn 220 11 to to IN cord-329245-6tj2k1yn 220 12 IBV IBV NNP cord-329245-6tj2k1yn 220 13 M M NNP cord-329245-6tj2k1yn 220 14 protein protein NN cord-329245-6tj2k1yn 220 15 ( ( -LRB- cord-329245-6tj2k1yn 220 16 Fig Fig NNP cord-329245-6tj2k1yn 220 17 . . . cord-329245-6tj2k1yn 220 18 4B 4b LS cord-329245-6tj2k1yn 220 19 ) ) -RRB- cord-329245-6tj2k1yn 220 20 , , , cord-329245-6tj2k1yn 220 21 but but CC cord-329245-6tj2k1yn 220 22 did do VBD cord-329245-6tj2k1yn 220 23 not not RB cord-329245-6tj2k1yn 220 24 induce induce VB cord-329245-6tj2k1yn 220 25 VLP VLP NNP cord-329245-6tj2k1yn 220 26 formation formation NN cord-329245-6tj2k1yn 220 27 ( ( -LRB- cord-329245-6tj2k1yn 220 28 Fig Fig NNP cord-329245-6tj2k1yn 220 29 . . . cord-329245-6tj2k1yn 220 30 6 6 CD cord-329245-6tj2k1yn 220 31 ) ) -RRB- cord-329245-6tj2k1yn 220 32 . . . cord-329245-6tj2k1yn 221 1 Since since IN cord-329245-6tj2k1yn 221 2 the the DT cord-329245-6tj2k1yn 221 3 GEt get NN cord-329245-6tj2k1yn 221 4 chimera chimera NN cord-329245-6tj2k1yn 221 5 contains contain VBZ cord-329245-6tj2k1yn 221 6 the the DT cord-329245-6tj2k1yn 221 7 same same JJ cord-329245-6tj2k1yn 221 8 transmembrane transmembrane NN cord-329245-6tj2k1yn 221 9 domain domain NN cord-329245-6tj2k1yn 221 10 and and CC cord-329245-6tj2k1yn 221 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 221 12 tail tail NN cord-329245-6tj2k1yn 221 13 as as IN cord-329245-6tj2k1yn 221 14 the the DT cord-329245-6tj2k1yn 221 15 transmembrane transmembrane NN cord-329245-6tj2k1yn 221 16 replacement replacement NN cord-329245-6tj2k1yn 221 17 mutant mutant NN cord-329245-6tj2k1yn 221 18 EG3 EG3 NNP cord-329245-6tj2k1yn 221 19 , , , cord-329245-6tj2k1yn 221 20 which which WDT cord-329245-6tj2k1yn 221 21 was be VBD cord-329245-6tj2k1yn 221 22 able able JJ cord-329245-6tj2k1yn 221 23 to to TO cord-329245-6tj2k1yn 221 24 induce induce VB cord-329245-6tj2k1yn 221 25 VLP VLP NNP cord-329245-6tj2k1yn 221 26 formation formation NN cord-329245-6tj2k1yn 221 27 as as RB cord-329245-6tj2k1yn 221 28 well well RB cord-329245-6tj2k1yn 221 29 as as IN cord-329245-6tj2k1yn 221 30 wild wild JJ cord-329245-6tj2k1yn 221 31 - - HYPH cord-329245-6tj2k1yn 221 32 type type NN cord-329245-6tj2k1yn 221 33 E e NN cord-329245-6tj2k1yn 221 34 protein protein NN cord-329245-6tj2k1yn 221 35 ( ( -LRB- cord-329245-6tj2k1yn 221 36 Fig Fig NNP cord-329245-6tj2k1yn 221 37 . . . cord-329245-6tj2k1yn 221 38 _SP cord-329245-6tj2k1yn 221 39 6 6 CD cord-329245-6tj2k1yn 221 40 ) ) -RRB- cord-329245-6tj2k1yn 221 41 , , , cord-329245-6tj2k1yn 221 42 a a DT cord-329245-6tj2k1yn 221 43 likely likely JJ cord-329245-6tj2k1yn 221 44 explanation explanation NN cord-329245-6tj2k1yn 221 45 is be VBZ cord-329245-6tj2k1yn 221 46 that that IN cord-329245-6tj2k1yn 221 47 the the DT cord-329245-6tj2k1yn 221 48 VSV VSV NNP cord-329245-6tj2k1yn 221 49 G G NNP cord-329245-6tj2k1yn 221 50 ectodomain ectodomain NN cord-329245-6tj2k1yn 221 51 prevents prevent VBZ cord-329245-6tj2k1yn 221 52 induction induction NN cord-329245-6tj2k1yn 221 53 of of IN cord-329245-6tj2k1yn 221 54 VLPs vlp NNS cord-329245-6tj2k1yn 221 55 . . . cord-329245-6tj2k1yn 222 1 One one CD cord-329245-6tj2k1yn 222 2 possibility possibility NN cord-329245-6tj2k1yn 222 3 is be VBZ cord-329245-6tj2k1yn 222 4 that that IN cord-329245-6tj2k1yn 222 5 this this DT cord-329245-6tj2k1yn 222 6 large large JJ cord-329245-6tj2k1yn 222 7 ectodomain ectodomain NN cord-329245-6tj2k1yn 222 8 does do VBZ cord-329245-6tj2k1yn 222 9 not not RB cord-329245-6tj2k1yn 222 10 fit fit VB cord-329245-6tj2k1yn 222 11 well well RB cord-329245-6tj2k1yn 222 12 into into IN cord-329245-6tj2k1yn 222 13 the the DT cord-329245-6tj2k1yn 222 14 lattice lattice NN cord-329245-6tj2k1yn 222 15 of of IN cord-329245-6tj2k1yn 222 16 M M NNP cord-329245-6tj2k1yn 222 17 protein protein NN cord-329245-6tj2k1yn 222 18 in in IN cord-329245-6tj2k1yn 222 19 the the DT cord-329245-6tj2k1yn 222 20 membrane membrane NN cord-329245-6tj2k1yn 222 21 . . . cord-329245-6tj2k1yn 223 1 A a DT cord-329245-6tj2k1yn 223 2 similar similar JJ cord-329245-6tj2k1yn 223 3 observation observation NN cord-329245-6tj2k1yn 223 4 was be VBD cord-329245-6tj2k1yn 223 5 reported report VBN cord-329245-6tj2k1yn 223 6 by by IN cord-329245-6tj2k1yn 223 7 Rottier Rottier NNP cord-329245-6tj2k1yn 223 8 and and CC cord-329245-6tj2k1yn 223 9 colleagues colleague NNS cord-329245-6tj2k1yn 223 10 , , , cord-329245-6tj2k1yn 223 11 who who WP cord-329245-6tj2k1yn 223 12 showed show VBD cord-329245-6tj2k1yn 223 13 that that IN cord-329245-6tj2k1yn 223 14 foreign foreign JJ cord-329245-6tj2k1yn 223 15 proteins protein NNS cord-329245-6tj2k1yn 223 16 such such JJ cord-329245-6tj2k1yn 223 17 as as IN cord-329245-6tj2k1yn 223 18 wild wild JJ cord-329245-6tj2k1yn 223 19 - - HYPH cord-329245-6tj2k1yn 223 20 type type NN cord-329245-6tj2k1yn 223 21 VSV VSV NNP cord-329245-6tj2k1yn 223 22 G G NNP cord-329245-6tj2k1yn 223 23 protein protein NN cord-329245-6tj2k1yn 223 24 , , , cord-329245-6tj2k1yn 223 25 equine equine JJ cord-329245-6tj2k1yn 223 26 arteritis arteritis NN cord-329245-6tj2k1yn 223 27 virus virus NN cord-329245-6tj2k1yn 223 28 M M NNP cord-329245-6tj2k1yn 223 29 protein protein NN cord-329245-6tj2k1yn 223 30 , , , cord-329245-6tj2k1yn 223 31 and and CC cord-329245-6tj2k1yn 223 32 CD8 CD8 NNP cord-329245-6tj2k1yn 223 33 were be VBD cord-329245-6tj2k1yn 223 34 effectively effectively RB cord-329245-6tj2k1yn 223 35 excluded exclude VBN cord-329245-6tj2k1yn 223 36 from from IN cord-329245-6tj2k1yn 223 37 MHV MHV NNP cord-329245-6tj2k1yn 223 38 VLPs vlp NNS cord-329245-6tj2k1yn 223 39 . . . cord-329245-6tj2k1yn 224 1 Likewise likewise RB cord-329245-6tj2k1yn 224 2 , , , cord-329245-6tj2k1yn 224 3 it -PRON- PRP cord-329245-6tj2k1yn 224 4 is be VBZ cord-329245-6tj2k1yn 224 5 possible possible JJ cord-329245-6tj2k1yn 224 6 that that IN cord-329245-6tj2k1yn 224 7 the the DT cord-329245-6tj2k1yn 224 8 M M NNP cord-329245-6tj2k1yn 224 9 deletion deletion NN cord-329245-6tj2k1yn 224 10 mutants mutant NNS cord-329245-6tj2k1yn 224 11 we -PRON- PRP cord-329245-6tj2k1yn 224 12 tested test VBD cord-329245-6tj2k1yn 224 13 in in IN cord-329245-6tj2k1yn 224 14 the the DT cord-329245-6tj2k1yn 224 15 VLP VLP NNP cord-329245-6tj2k1yn 224 16 assay assay NN cord-329245-6tj2k1yn 224 17 are be VBP cord-329245-6tj2k1yn 224 18 not not RB cord-329245-6tj2k1yn 224 19 in in IN cord-329245-6tj2k1yn 224 20 the the DT cord-329245-6tj2k1yn 224 21 right right JJ cord-329245-6tj2k1yn 224 22 conformation conformation NN cord-329245-6tj2k1yn 224 23 to to TO cord-329245-6tj2k1yn 224 24 fit fit VB cord-329245-6tj2k1yn 224 25 into into IN cord-329245-6tj2k1yn 224 26 such such PDT cord-329245-6tj2k1yn 224 27 a a DT cord-329245-6tj2k1yn 224 28 lattice lattice NN cord-329245-6tj2k1yn 224 29 of of IN cord-329245-6tj2k1yn 224 30 M M NNP cord-329245-6tj2k1yn 224 31 protein protein NN cord-329245-6tj2k1yn 224 32 , , , cord-329245-6tj2k1yn 224 33 and and CC cord-329245-6tj2k1yn 224 34 thus thus RB cord-329245-6tj2k1yn 224 35 , , , cord-329245-6tj2k1yn 224 36 do do VBP cord-329245-6tj2k1yn 224 37 not not RB cord-329245-6tj2k1yn 224 38 support support VB cord-329245-6tj2k1yn 224 39 VLP VLP NNP cord-329245-6tj2k1yn 224 40 formation formation NN cord-329245-6tj2k1yn 224 41 . . . cord-329245-6tj2k1yn 225 1 As as IN cord-329245-6tj2k1yn 225 2 mentioned mention VBN cord-329245-6tj2k1yn 225 3 above above RB cord-329245-6tj2k1yn 225 4 , , , cord-329245-6tj2k1yn 225 5 we -PRON- PRP cord-329245-6tj2k1yn 225 6 have have VBP cord-329245-6tj2k1yn 225 7 previously previously RB cord-329245-6tj2k1yn 225 8 shown show VBN cord-329245-6tj2k1yn 225 9 that that IN cord-329245-6tj2k1yn 225 10 the the DT cord-329245-6tj2k1yn 225 11 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 225 12 tail tail NN cord-329245-6tj2k1yn 225 13 of of IN cord-329245-6tj2k1yn 225 14 the the DT cord-329245-6tj2k1yn 225 15 IBV IBV NNP cord-329245-6tj2k1yn 225 16 E E NNP cord-329245-6tj2k1yn 225 17 protein protein NN cord-329245-6tj2k1yn 225 18 is be VBZ cord-329245-6tj2k1yn 225 19 sufficient sufficient JJ cord-329245-6tj2k1yn 225 20 to to TO cord-329245-6tj2k1yn 225 21 localize localize VB cord-329245-6tj2k1yn 225 22 it -PRON- PRP cord-329245-6tj2k1yn 225 23 to to IN cord-329245-6tj2k1yn 225 24 the the DT cord-329245-6tj2k1yn 225 25 Golgi Golgi NNP cord-329245-6tj2k1yn 225 26 complex complex NN cord-329245-6tj2k1yn 225 27 and and CC cord-329245-6tj2k1yn 225 28 to to TO cord-329245-6tj2k1yn 225 29 mediate mediate VB cord-329245-6tj2k1yn 225 30 its -PRON- PRP$ cord-329245-6tj2k1yn 225 31 redistribution redistribution NN cord-329245-6tj2k1yn 225 32 with with IN cord-329245-6tj2k1yn 225 33 the the DT cord-329245-6tj2k1yn 225 34 Golgi Golgi NNP cord-329245-6tj2k1yn 225 35 scaffold scaffold NN cord-329245-6tj2k1yn 225 36 proteins protein NNS cord-329245-6tj2k1yn 225 37 GM130 GM130 NNP cord-329245-6tj2k1yn 225 38 and and CC cord-329245-6tj2k1yn 225 39 p115 p115 NNS cord-329245-6tj2k1yn 225 40 during during IN cord-329245-6tj2k1yn 225 41 brefeldin brefeldin NNP cord-329245-6tj2k1yn 225 42 - - : cord-329245-6tj2k1yn 225 43 A a DT cord-329245-6tj2k1yn 225 44 treatment treatment NN cord-329245-6tj2k1yn 225 45 ( ( -LRB- cord-329245-6tj2k1yn 225 46 Corse Corse NNP cord-329245-6tj2k1yn 225 47 and and CC cord-329245-6tj2k1yn 225 48 Machamer Machamer NNP cord-329245-6tj2k1yn 225 49 , , , cord-329245-6tj2k1yn 225 50 2002 2002 CD cord-329245-6tj2k1yn 225 51 ) ) -RRB- cord-329245-6tj2k1yn 225 52 . . . cord-329245-6tj2k1yn 226 1 Since since IN cord-329245-6tj2k1yn 226 2 these these DT cord-329245-6tj2k1yn 226 3 and and CC cord-329245-6tj2k1yn 226 4 other other JJ cord-329245-6tj2k1yn 226 5 Golgi Golgi NNP cord-329245-6tj2k1yn 226 6 scaffold scaffold NN cord-329245-6tj2k1yn 226 7 proteins protein NNS cord-329245-6tj2k1yn 226 8 are be VBP cord-329245-6tj2k1yn 226 9 known know VBN cord-329245-6tj2k1yn 226 10 to to TO cord-329245-6tj2k1yn 226 11 be be VB cord-329245-6tj2k1yn 226 12 part part NN cord-329245-6tj2k1yn 226 13 of of IN cord-329245-6tj2k1yn 226 14 a a DT cord-329245-6tj2k1yn 226 15 complex complex NN cord-329245-6tj2k1yn 226 16 which which WDT cord-329245-6tj2k1yn 226 17 is be VBZ cord-329245-6tj2k1yn 226 18 thought think VBN cord-329245-6tj2k1yn 226 19 to to TO cord-329245-6tj2k1yn 226 20 mediate mediate VB cord-329245-6tj2k1yn 226 21 vesicle vesicle NN cord-329245-6tj2k1yn 226 22 tethering tethering NN cord-329245-6tj2k1yn 226 23 during during IN cord-329245-6tj2k1yn 226 24 intra intra JJ cord-329245-6tj2k1yn 226 25 - - JJ cord-329245-6tj2k1yn 226 26 Golgi golgi JJ cord-329245-6tj2k1yn 226 27 transport transport NN cord-329245-6tj2k1yn 227 1 ( ( -LRB- cord-329245-6tj2k1yn 227 2 Barr Barr NNP cord-329245-6tj2k1yn 227 3 et et NNP cord-329245-6tj2k1yn 227 4 al al NNP cord-329245-6tj2k1yn 227 5 . . . cord-329245-6tj2k1yn 228 1 , , , cord-329245-6tj2k1yn 228 2 1998 1998 CD cord-329245-6tj2k1yn 228 3 ; ; : cord-329245-6tj2k1yn 228 4 Seemann Seemann NNP cord-329245-6tj2k1yn 228 5 et et NNP cord-329245-6tj2k1yn 228 6 al al NNP cord-329245-6tj2k1yn 228 7 . . NNP cord-329245-6tj2k1yn 228 8 , , , cord-329245-6tj2k1yn 228 9 2000a 2000a CD cord-329245-6tj2k1yn 228 10 , , , cord-329245-6tj2k1yn 228 11 b b LS cord-329245-6tj2k1yn 228 12 ) ) -RRB- cord-329245-6tj2k1yn 228 13 , , , cord-329245-6tj2k1yn 228 14 this this DT cord-329245-6tj2k1yn 228 15 led lead VBD cord-329245-6tj2k1yn 228 16 us -PRON- PRP cord-329245-6tj2k1yn 228 17 to to TO cord-329245-6tj2k1yn 228 18 hypothesize hypothesize VB cord-329245-6tj2k1yn 228 19 that that IN cord-329245-6tj2k1yn 228 20 the the DT cord-329245-6tj2k1yn 228 21 IBV IBV NNP cord-329245-6tj2k1yn 228 22 E E NNP cord-329245-6tj2k1yn 228 23 protein protein NN cord-329245-6tj2k1yn 228 24 may may MD cord-329245-6tj2k1yn 228 25 be be VB cord-329245-6tj2k1yn 228 26 targeted target VBN cord-329245-6tj2k1yn 228 27 to to IN cord-329245-6tj2k1yn 228 28 the the DT cord-329245-6tj2k1yn 228 29 Golgi Golgi NNP cord-329245-6tj2k1yn 228 30 apparatus apparatus NN cord-329245-6tj2k1yn 228 31 via via IN cord-329245-6tj2k1yn 228 32 a a DT cord-329245-6tj2k1yn 228 33 direct direct JJ cord-329245-6tj2k1yn 228 34 interaction interaction NN cord-329245-6tj2k1yn 228 35 with with IN cord-329245-6tj2k1yn 228 36 one one CD cord-329245-6tj2k1yn 228 37 or or CC cord-329245-6tj2k1yn 228 38 more more JJR cord-329245-6tj2k1yn 228 39 Golgi Golgi NNP cord-329245-6tj2k1yn 228 40 scaffold scaffold NN cord-329245-6tj2k1yn 228 41 proteins protein NNS cord-329245-6tj2k1yn 228 42 . . . cord-329245-6tj2k1yn 229 1 Furthermore furthermore RB cord-329245-6tj2k1yn 229 2 , , , cord-329245-6tj2k1yn 229 3 interaction interaction NN cord-329245-6tj2k1yn 229 4 of of IN cord-329245-6tj2k1yn 229 5 IBV IBV NNP cord-329245-6tj2k1yn 229 6 E E NNP cord-329245-6tj2k1yn 229 7 with with IN cord-329245-6tj2k1yn 229 8 the the DT cord-329245-6tj2k1yn 229 9 Golgi Golgi NNP cord-329245-6tj2k1yn 229 10 scaffold scaffold NN cord-329245-6tj2k1yn 229 11 could could MD cord-329245-6tj2k1yn 229 12 result result VB cord-329245-6tj2k1yn 229 13 in in IN cord-329245-6tj2k1yn 229 14 modulation modulation NN cord-329245-6tj2k1yn 229 15 of of IN cord-329245-6tj2k1yn 229 16 vesicular vesicular JJ cord-329245-6tj2k1yn 229 17 traffic traffic NN cord-329245-6tj2k1yn 229 18 , , , cord-329245-6tj2k1yn 229 19 which which WDT cord-329245-6tj2k1yn 229 20 might may MD cord-329245-6tj2k1yn 229 21 be be VB cord-329245-6tj2k1yn 229 22 advantageous advantageous JJ cord-329245-6tj2k1yn 229 23 for for IN cord-329245-6tj2k1yn 229 24 collecting collect VBG cord-329245-6tj2k1yn 229 25 coronavirus coronavirus NN cord-329245-6tj2k1yn 229 26 envelope envelope NN cord-329245-6tj2k1yn 229 27 proteins protein NNS cord-329245-6tj2k1yn 229 28 in in IN cord-329245-6tj2k1yn 229 29 a a DT cord-329245-6tj2k1yn 229 30 specific specific JJ cord-329245-6tj2k1yn 229 31 Golgi Golgi NNP cord-329245-6tj2k1yn 229 32 compartment compartment NN cord-329245-6tj2k1yn 229 33 for for IN cord-329245-6tj2k1yn 229 34 assembly assembly NN cord-329245-6tj2k1yn 229 35 ( ( -LRB- cord-329245-6tj2k1yn 229 36 Corse Corse NNP cord-329245-6tj2k1yn 229 37 and and CC cord-329245-6tj2k1yn 229 38 Machamer Machamer NNP cord-329245-6tj2k1yn 229 39 , , , cord-329245-6tj2k1yn 229 40 2002 2002 CD cord-329245-6tj2k1yn 229 41 ) ) -RRB- cord-329245-6tj2k1yn 229 42 . . . cord-329245-6tj2k1yn 230 1 Since since IN cord-329245-6tj2k1yn 230 2 we -PRON- PRP cord-329245-6tj2k1yn 230 3 have have VBP cord-329245-6tj2k1yn 230 4 shown show VBN cord-329245-6tj2k1yn 230 5 here here RB cord-329245-6tj2k1yn 230 6 that that IN cord-329245-6tj2k1yn 230 7 the the DT cord-329245-6tj2k1yn 230 8 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 230 9 tail tail NN cord-329245-6tj2k1yn 230 10 of of IN cord-329245-6tj2k1yn 230 11 IBV IBV NNP cord-329245-6tj2k1yn 230 12 E E NNP cord-329245-6tj2k1yn 230 13 is be VBZ cord-329245-6tj2k1yn 230 14 also also RB cord-329245-6tj2k1yn 230 15 important important JJ cord-329245-6tj2k1yn 230 16 for for IN cord-329245-6tj2k1yn 230 17 interactions interaction NNS cord-329245-6tj2k1yn 230 18 with with IN cord-329245-6tj2k1yn 230 19 IBV IBV NNP cord-329245-6tj2k1yn 230 20 M M NNP cord-329245-6tj2k1yn 230 21 , , , cord-329245-6tj2k1yn 230 22 this this DT cord-329245-6tj2k1yn 230 23 raises raise VBZ cord-329245-6tj2k1yn 230 24 the the DT cord-329245-6tj2k1yn 230 25 question question NN cord-329245-6tj2k1yn 230 26 of of IN cord-329245-6tj2k1yn 230 27 whether whether IN cord-329245-6tj2k1yn 230 28 the the DT cord-329245-6tj2k1yn 230 29 E e NN cord-329245-6tj2k1yn 230 30 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 230 31 tail tail NN cord-329245-6tj2k1yn 230 32 can can MD cord-329245-6tj2k1yn 230 33 interact interact VB cord-329245-6tj2k1yn 230 34 with with IN cord-329245-6tj2k1yn 230 35 M M NNP cord-329245-6tj2k1yn 230 36 and and CC cord-329245-6tj2k1yn 230 37 the the DT cord-329245-6tj2k1yn 230 38 Golgi Golgi NNP cord-329245-6tj2k1yn 230 39 scaffold scaffold NN cord-329245-6tj2k1yn 230 40 simultaneously simultaneously RB cord-329245-6tj2k1yn 230 41 . . . cord-329245-6tj2k1yn 231 1 If if IN cord-329245-6tj2k1yn 231 2 not not RB cord-329245-6tj2k1yn 231 3 , , , cord-329245-6tj2k1yn 231 4 then then RB cord-329245-6tj2k1yn 231 5 it -PRON- PRP cord-329245-6tj2k1yn 231 6 is be VBZ cord-329245-6tj2k1yn 231 7 possible possible JJ cord-329245-6tj2k1yn 231 8 that that IN cord-329245-6tj2k1yn 231 9 two two CD cord-329245-6tj2k1yn 231 10 functional functional JJ cord-329245-6tj2k1yn 231 11 pools pool NNS cord-329245-6tj2k1yn 231 12 of of IN cord-329245-6tj2k1yn 231 13 IBV IBV NNP cord-329245-6tj2k1yn 231 14 E E NNP cord-329245-6tj2k1yn 231 15 protein protein NN cord-329245-6tj2k1yn 231 16 exist exist VBP cord-329245-6tj2k1yn 231 17 in in IN cord-329245-6tj2k1yn 231 18 an an DT cord-329245-6tj2k1yn 231 19 infected infected JJ cord-329245-6tj2k1yn 231 20 cell cell NN cord-329245-6tj2k1yn 231 21 . . . cord-329245-6tj2k1yn 232 1 One one CD cord-329245-6tj2k1yn 232 2 pool pool NN cord-329245-6tj2k1yn 232 3 could could MD cord-329245-6tj2k1yn 232 4 interact interact VB cord-329245-6tj2k1yn 232 5 with with IN cord-329245-6tj2k1yn 232 6 IBV IBV NNP cord-329245-6tj2k1yn 232 7 M M NNP cord-329245-6tj2k1yn 232 8 and and CC cord-329245-6tj2k1yn 232 9 the the DT cord-329245-6tj2k1yn 232 10 membrane membrane NN cord-329245-6tj2k1yn 232 11 at at IN cord-329245-6tj2k1yn 232 12 the the DT cord-329245-6tj2k1yn 232 13 assembly assembly NN cord-329245-6tj2k1yn 232 14 site site NN cord-329245-6tj2k1yn 232 15 to to TO cord-329245-6tj2k1yn 232 16 induce induce VB cord-329245-6tj2k1yn 232 17 virus virus NN cord-329245-6tj2k1yn 232 18 budding bud VBG cord-329245-6tj2k1yn 232 19 , , , cord-329245-6tj2k1yn 232 20 while while IN cord-329245-6tj2k1yn 232 21 the the DT cord-329245-6tj2k1yn 232 22 other other JJ cord-329245-6tj2k1yn 232 23 could could MD cord-329245-6tj2k1yn 232 24 associate associate VB cord-329245-6tj2k1yn 232 25 with with IN cord-329245-6tj2k1yn 232 26 the the DT cord-329245-6tj2k1yn 232 27 Golgi Golgi NNP cord-329245-6tj2k1yn 232 28 scaffold scaffold NN cord-329245-6tj2k1yn 232 29 to to TO cord-329245-6tj2k1yn 232 30 influence influence VB cord-329245-6tj2k1yn 232 31 vesicular vesicular JJ cord-329245-6tj2k1yn 232 32 traffic traffic NN cord-329245-6tj2k1yn 232 33 through through IN cord-329245-6tj2k1yn 232 34 the the DT cord-329245-6tj2k1yn 232 35 Golgi Golgi NNP cord-329245-6tj2k1yn 232 36 complex complex NN cord-329245-6tj2k1yn 232 37 . . . cord-329245-6tj2k1yn 233 1 The the DT cord-329245-6tj2k1yn 233 2 latter latter JJ cord-329245-6tj2k1yn 233 3 interaction interaction NN cord-329245-6tj2k1yn 233 4 might may MD cord-329245-6tj2k1yn 233 5 aid aid VB cord-329245-6tj2k1yn 233 6 in in IN cord-329245-6tj2k1yn 233 7 collecting collect VBG cord-329245-6tj2k1yn 233 8 viral viral JJ cord-329245-6tj2k1yn 233 9 membrane membrane NN cord-329245-6tj2k1yn 233 10 proteins protein NNS cord-329245-6tj2k1yn 233 11 for for IN cord-329245-6tj2k1yn 233 12 assembly assembly NN cord-329245-6tj2k1yn 233 13 at at IN cord-329245-6tj2k1yn 233 14 Golgi Golgi NNP cord-329245-6tj2k1yn 233 15 membranes membrane NNS cord-329245-6tj2k1yn 233 16 . . . cord-329245-6tj2k1yn 234 1 Since since IN cord-329245-6tj2k1yn 234 2 the the DT cord-329245-6tj2k1yn 234 3 small small JJ cord-329245-6tj2k1yn 234 4 amount amount NN cord-329245-6tj2k1yn 234 5 of of IN cord-329245-6tj2k1yn 234 6 IBV IBV NNP cord-329245-6tj2k1yn 234 7 E E NNP cord-329245-6tj2k1yn 234 8 protein protein NN cord-329245-6tj2k1yn 234 9 found find VBN cord-329245-6tj2k1yn 234 10 in in IN cord-329245-6tj2k1yn 234 11 virions virion NNS cord-329245-6tj2k1yn 234 12 ( ( -LRB- cord-329245-6tj2k1yn 234 13 Liu Liu NNP cord-329245-6tj2k1yn 234 14 and and CC cord-329245-6tj2k1yn 234 15 Inglis Inglis NNP cord-329245-6tj2k1yn 234 16 , , , cord-329245-6tj2k1yn 234 17 1991 1991 CD cord-329245-6tj2k1yn 234 18 ) ) -RRB- cord-329245-6tj2k1yn 234 19 and and CC cord-329245-6tj2k1yn 234 20 VLPs vlp NNS cord-329245-6tj2k1yn 234 21 ( ( -LRB- cord-329245-6tj2k1yn 234 22 shown show VBN cord-329245-6tj2k1yn 234 23 here here RB cord-329245-6tj2k1yn 234 24 ) ) -RRB- cord-329245-6tj2k1yn 234 25 suggests suggest VBZ cord-329245-6tj2k1yn 234 26 that that IN cord-329245-6tj2k1yn 234 27 not not RB cord-329245-6tj2k1yn 234 28 much much JJ cord-329245-6tj2k1yn 234 29 E e NN cord-329245-6tj2k1yn 234 30 protein protein NN cord-329245-6tj2k1yn 234 31 is be VBZ cord-329245-6tj2k1yn 234 32 required require VBN cord-329245-6tj2k1yn 234 33 to to TO cord-329245-6tj2k1yn 234 34 induce induce VB cord-329245-6tj2k1yn 234 35 virus virus NN cord-329245-6tj2k1yn 234 36 budding bud VBG cord-329245-6tj2k1yn 234 37 , , , cord-329245-6tj2k1yn 234 38 maybe maybe RB cord-329245-6tj2k1yn 234 39 a a DT cord-329245-6tj2k1yn 234 40 second second JJ cord-329245-6tj2k1yn 234 41 function function NN cord-329245-6tj2k1yn 234 42 of of IN cord-329245-6tj2k1yn 234 43 E e NN cord-329245-6tj2k1yn 234 44 protein protein NN cord-329245-6tj2k1yn 234 45 , , , cord-329245-6tj2k1yn 234 46 such such JJ cord-329245-6tj2k1yn 234 47 as as IN cord-329245-6tj2k1yn 234 48 modulation modulation NN cord-329245-6tj2k1yn 234 49 of of IN cord-329245-6tj2k1yn 234 50 Golgi Golgi NNP cord-329245-6tj2k1yn 234 51 transport transport NN cord-329245-6tj2k1yn 234 52 , , , cord-329245-6tj2k1yn 234 53 accounts account VBZ cord-329245-6tj2k1yn 234 54 for for IN cord-329245-6tj2k1yn 234 55 the the DT cord-329245-6tj2k1yn 234 56 apparent apparent JJ cord-329245-6tj2k1yn 234 57 excess excess NN cord-329245-6tj2k1yn 234 58 of of IN cord-329245-6tj2k1yn 234 59 IBV IBV NNP cord-329245-6tj2k1yn 234 60 E E NNP cord-329245-6tj2k1yn 234 61 protein protein NN cord-329245-6tj2k1yn 234 62 in in IN cord-329245-6tj2k1yn 234 63 infected infected JJ cord-329245-6tj2k1yn 234 64 cells cell NNS cord-329245-6tj2k1yn 234 65 ( ( -LRB- cord-329245-6tj2k1yn 234 66 Corse Corse NNP cord-329245-6tj2k1yn 234 67 and and CC cord-329245-6tj2k1yn 234 68 Machamer Machamer NNP cord-329245-6tj2k1yn 234 69 , , , cord-329245-6tj2k1yn 234 70 2000 2000 CD cord-329245-6tj2k1yn 234 71 ) ) -RRB- cord-329245-6tj2k1yn 234 72 . . . cord-329245-6tj2k1yn 235 1 OST7 OST7 NNP cord-329245-6tj2k1yn 235 2 - - HYPH cord-329245-6tj2k1yn 235 3 1 1 CD cord-329245-6tj2k1yn 235 4 cells cell NNS cord-329245-6tj2k1yn 235 5 ( ( -LRB- cord-329245-6tj2k1yn 235 6 Elroy Elroy NNP cord-329245-6tj2k1yn 235 7 - - HYPH cord-329245-6tj2k1yn 235 8 Stein Stein NNP cord-329245-6tj2k1yn 235 9 and and CC cord-329245-6tj2k1yn 235 10 Moss Moss NNP cord-329245-6tj2k1yn 235 11 , , , cord-329245-6tj2k1yn 235 12 1990 1990 CD cord-329245-6tj2k1yn 235 13 ) ) -RRB- cord-329245-6tj2k1yn 236 1 and and CC cord-329245-6tj2k1yn 236 2 Vero Vero NNP cord-329245-6tj2k1yn 236 3 cells cell NNS cord-329245-6tj2k1yn 236 4 were be VBD cord-329245-6tj2k1yn 236 5 maintained maintain VBN cord-329245-6tj2k1yn 236 6 in in IN cord-329245-6tj2k1yn 236 7 Dulbecco Dulbecco NNP cord-329245-6tj2k1yn 236 8 's 's POS cord-329245-6tj2k1yn 236 9 modified modify VBN cord-329245-6tj2k1yn 236 10 Eagle Eagle NNP cord-329245-6tj2k1yn 236 11 's 's POS cord-329245-6tj2k1yn 236 12 medium medium NN cord-329245-6tj2k1yn 236 13 ( ( -LRB- cord-329245-6tj2k1yn 236 14 DMEM DMEM NNP cord-329245-6tj2k1yn 236 15 ) ) -RRB- cord-329245-6tj2k1yn 236 16 containing contain VBG cord-329245-6tj2k1yn 236 17 10 10 CD cord-329245-6tj2k1yn 236 18 % % NN cord-329245-6tj2k1yn 236 19 fetal fetal JJ cord-329245-6tj2k1yn 236 20 calf calf NN cord-329245-6tj2k1yn 236 21 serum serum NN cord-329245-6tj2k1yn 236 22 ( ( -LRB- cord-329245-6tj2k1yn 236 23 FCS FCS NNP cord-329245-6tj2k1yn 236 24 ) ) -RRB- cord-329245-6tj2k1yn 236 25 and and CC cord-329245-6tj2k1yn 236 26 antibiotics antibiotic NNS cord-329245-6tj2k1yn 236 27 . . . cord-329245-6tj2k1yn 237 1 The the DT cord-329245-6tj2k1yn 237 2 adaptation adaptation NN cord-329245-6tj2k1yn 237 3 of of IN cord-329245-6tj2k1yn 237 4 IBV IBV NNP cord-329245-6tj2k1yn 237 5 ( ( -LRB- cord-329245-6tj2k1yn 237 6 Beaudette Beaudette NNP cord-329245-6tj2k1yn 237 7 strain strain NN cord-329245-6tj2k1yn 237 8 ) ) -RRB- cord-329245-6tj2k1yn 237 9 to to IN cord-329245-6tj2k1yn 237 10 Vero Vero NNP cord-329245-6tj2k1yn 237 11 cells cell NNS cord-329245-6tj2k1yn 237 12 has have VBZ cord-329245-6tj2k1yn 237 13 been be VBN cord-329245-6tj2k1yn 237 14 described describe VBN cord-329245-6tj2k1yn 237 15 ( ( -LRB- cord-329245-6tj2k1yn 237 16 Machamer Machamer NNP cord-329245-6tj2k1yn 237 17 and and CC cord-329245-6tj2k1yn 237 18 Rose Rose NNP cord-329245-6tj2k1yn 237 19 , , , cord-329245-6tj2k1yn 237 20 1987 1987 CD cord-329245-6tj2k1yn 237 21 ) ) -RRB- cord-329245-6tj2k1yn 237 22 . . . cord-329245-6tj2k1yn 238 1 The the DT cord-329245-6tj2k1yn 238 2 recombinant recombinant JJ cord-329245-6tj2k1yn 238 3 vaccinia vaccinia NN cord-329245-6tj2k1yn 238 4 viruses virus NNS cord-329245-6tj2k1yn 238 5 encoding encode VBG cord-329245-6tj2k1yn 238 6 phage phage NN cord-329245-6tj2k1yn 238 7 T7 t7 NN cord-329245-6tj2k1yn 238 8 polymerase polymerase NN cord-329245-6tj2k1yn 238 9 ( ( -LRB- cord-329245-6tj2k1yn 238 10 vTF7 vTF7 NNP cord-329245-6tj2k1yn 238 11 - - HYPH cord-329245-6tj2k1yn 238 12 3 3 CD cord-329245-6tj2k1yn 238 13 ) ) -RRB- cord-329245-6tj2k1yn 238 14 , , , cord-329245-6tj2k1yn 238 15 IBV IBV NNP cord-329245-6tj2k1yn 238 16 M M NNP cord-329245-6tj2k1yn 238 17 ( ( -LRB- cord-329245-6tj2k1yn 238 18 vvIBVM vvIBVM NNP cord-329245-6tj2k1yn 238 19 ) ) -RRB- cord-329245-6tj2k1yn 238 20 , , , cord-329245-6tj2k1yn 238 21 and and CC cord-329245-6tj2k1yn 238 22 wild wild JJ cord-329245-6tj2k1yn 238 23 - - HYPH cord-329245-6tj2k1yn 238 24 type type NN cord-329245-6tj2k1yn 238 25 and and CC cord-329245-6tj2k1yn 238 26 mutant mutant JJ cord-329245-6tj2k1yn 238 27 versions version NNS cord-329245-6tj2k1yn 238 28 of of IN cord-329245-6tj2k1yn 238 29 IBV IBV NNP cord-329245-6tj2k1yn 238 30 E E NNP cord-329245-6tj2k1yn 238 31 ( ( -LRB- cord-329245-6tj2k1yn 238 32 vvIBVE vvibve UH cord-329245-6tj2k1yn 238 33 , , , cord-329245-6tj2k1yn 238 34 vvEG3 vvEG3 NNP cord-329245-6tj2k1yn 238 35 , , , cord-329245-6tj2k1yn 238 36 vvCTE vvcte NN cord-329245-6tj2k1yn 238 37 ) ) -RRB- cord-329245-6tj2k1yn 238 38 have have VBP cord-329245-6tj2k1yn 238 39 been be VBN cord-329245-6tj2k1yn 238 40 described describe VBN cord-329245-6tj2k1yn 238 41 Machamer Machamer NNP cord-329245-6tj2k1yn 238 42 , , , cord-329245-6tj2k1yn 238 43 2000 2000 CD cord-329245-6tj2k1yn 238 44 , , , cord-329245-6tj2k1yn 238 45 2002 2002 CD cord-329245-6tj2k1yn 238 46 ; ; : cord-329245-6tj2k1yn 239 1 Fuerst Fuerst NNP cord-329245-6tj2k1yn 239 2 et et NNP cord-329245-6tj2k1yn 239 3 al al NNP cord-329245-6tj2k1yn 239 4 . . NNP cord-329245-6tj2k1yn 239 5 , , , cord-329245-6tj2k1yn 239 6 1986 1986 CD cord-329245-6tj2k1yn 239 7 ; ; : cord-329245-6tj2k1yn 239 8 Machamer Machamer NNP cord-329245-6tj2k1yn 239 9 and and CC cord-329245-6tj2k1yn 239 10 Rose Rose NNP cord-329245-6tj2k1yn 239 11 , , , cord-329245-6tj2k1yn 239 12 1987 1987 CD cord-329245-6tj2k1yn 239 13 ) ) -RRB- cord-329245-6tj2k1yn 239 14 . . . cord-329245-6tj2k1yn 240 1 Growth growth NN cord-329245-6tj2k1yn 240 2 and and CC cord-329245-6tj2k1yn 240 3 titering titering NN cord-329245-6tj2k1yn 240 4 of of IN cord-329245-6tj2k1yn 240 5 recombinant recombinant JJ cord-329245-6tj2k1yn 240 6 vaccinia vaccinia NN cord-329245-6tj2k1yn 240 7 viruses virus NNS cord-329245-6tj2k1yn 240 8 were be VBD cord-329245-6tj2k1yn 240 9 done do VBN cord-329245-6tj2k1yn 240 10 as as IN cord-329245-6tj2k1yn 240 11 described describe VBN cord-329245-6tj2k1yn 240 12 ( ( -LRB- cord-329245-6tj2k1yn 240 13 Weisz Weisz NNP cord-329245-6tj2k1yn 240 14 and and CC cord-329245-6tj2k1yn 240 15 Machamer Machamer NNP cord-329245-6tj2k1yn 240 16 , , , cord-329245-6tj2k1yn 240 17 1994 1994 CD cord-329245-6tj2k1yn 240 18 ) ) -RRB- cord-329245-6tj2k1yn 240 19 . . . cord-329245-6tj2k1yn 241 1 The the DT cord-329245-6tj2k1yn 241 2 pBS pbs JJ cord-329245-6tj2k1yn 241 3 / / SYM cord-329245-6tj2k1yn 241 4 IBV IBV NNP cord-329245-6tj2k1yn 241 5 E E NNP cord-329245-6tj2k1yn 241 6 , , , cord-329245-6tj2k1yn 241 7 pBS pBS NNP cord-329245-6tj2k1yn 241 8 / / SYM cord-329245-6tj2k1yn 241 9 EG1 EG1 NNP cord-329245-6tj2k1yn 241 10 , , , cord-329245-6tj2k1yn 241 11 pBS pBS NNP cord-329245-6tj2k1yn 241 12 / / SYM cord-329245-6tj2k1yn 241 13 EG2 EG2 NNP cord-329245-6tj2k1yn 241 14 , , , cord-329245-6tj2k1yn 241 15 pBS pBS NNP cord-329245-6tj2k1yn 241 16 / / SYM cord-329245-6tj2k1yn 241 17 EG3 EG3 NNP cord-329245-6tj2k1yn 241 18 , , , cord-329245-6tj2k1yn 241 19 pBS/ pBS/ NNS cord-329245-6tj2k1yn 241 20 GEt get VBP cord-329245-6tj2k1yn 241 21 , , , cord-329245-6tj2k1yn 241 22 pAR pAR NNP cord-329245-6tj2k1yn 241 23 / / SYM cord-329245-6tj2k1yn 241 24 M M NNP cord-329245-6tj2k1yn 241 25 and and CC cord-329245-6tj2k1yn 241 26 pAR pAR NNP cord-329245-6tj2k1yn 242 1 /M /M . cord-329245-6tj2k1yn 242 2 ⌬ ⌬ NNP cord-329245-6tj2k1yn 242 3 m2,3 m2,3 NNP cord-329245-6tj2k1yn 242 4 plasmids plasmid NNS cord-329245-6tj2k1yn 242 5 have have VBP cord-329245-6tj2k1yn 242 6 been be VBN cord-329245-6tj2k1yn 242 7 described describe VBN cord-329245-6tj2k1yn 242 8 Machamer Machamer NNP cord-329245-6tj2k1yn 242 9 , , , cord-329245-6tj2k1yn 242 10 2000 2000 CD cord-329245-6tj2k1yn 242 11 , , , cord-329245-6tj2k1yn 242 12 2002 2002 CD cord-329245-6tj2k1yn 242 13 ; ; : cord-329245-6tj2k1yn 242 14 Machamer Machamer NNP cord-329245-6tj2k1yn 242 15 and and CC cord-329245-6tj2k1yn 242 16 Rose Rose NNP cord-329245-6tj2k1yn 242 17 , , , cord-329245-6tj2k1yn 242 18 1987 1987 CD cord-329245-6tj2k1yn 242 19 ) ) -RRB- cord-329245-6tj2k1yn 242 20 . . . cord-329245-6tj2k1yn 243 1 IBV IBV NNP cord-329245-6tj2k1yn 243 2 M M NNP cord-329245-6tj2k1yn 243 3 was be VBD cord-329245-6tj2k1yn 243 4 subcloned subclone VBN cord-329245-6tj2k1yn 243 5 into into IN cord-329245-6tj2k1yn 243 6 the the DT cord-329245-6tj2k1yn 243 7 BamHI BamHI NNP cord-329245-6tj2k1yn 243 8 site site NN cord-329245-6tj2k1yn 243 9 of of IN cord-329245-6tj2k1yn 243 10 pT7 pT7 NNP cord-329245-6tj2k1yn 243 11 / / SYM cord-329245-6tj2k1yn 243 12 T3 T3 NNP cord-329245-6tj2k1yn 243 13 - - HYPH cord-329245-6tj2k1yn 243 14 18 18 CD cord-329245-6tj2k1yn 243 15 ( ( -LRB- cord-329245-6tj2k1yn 243 16 BRL BRL NNP cord-329245-6tj2k1yn 243 17 ) ) -RRB- cord-329245-6tj2k1yn 243 18 to to TO cord-329245-6tj2k1yn 243 19 create create VB cord-329245-6tj2k1yn 243 20 pT7 pt7 RB cord-329245-6tj2k1yn 243 21 / / SYM cord-329245-6tj2k1yn 243 22 M. m. NN cord-329245-6tj2k1yn 244 1 The the DT cord-329245-6tj2k1yn 244 2 M M NNP cord-329245-6tj2k1yn 244 3 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 244 4 tail tail NN cord-329245-6tj2k1yn 244 5 deletion deletion NN cord-329245-6tj2k1yn 244 6 mutant mutant NN cord-329245-6tj2k1yn 244 7 plasmids plasmid NNS cord-329245-6tj2k1yn 244 8 pAR pAR NNP cord-329245-6tj2k1yn 244 9 / / , cord-329245-6tj2k1yn 244 10 Mct Mct NNP cord-329245-6tj2k1yn 244 11 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 244 12 1 1 CD cord-329245-6tj2k1yn 244 13 , , , cord-329245-6tj2k1yn 244 14 pAR par NN cord-329245-6tj2k1yn 244 15 / / SYM cord-329245-6tj2k1yn 244 16 Mct Mct NNP cord-329245-6tj2k1yn 244 17 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 244 18 2 2 CD cord-329245-6tj2k1yn 244 19 , , , cord-329245-6tj2k1yn 244 20 and and CC cord-329245-6tj2k1yn 244 21 pAR par NN cord-329245-6tj2k1yn 245 1 /Mct /Mct NNP cord-329245-6tj2k1yn 245 2 ⌬ ⌬ NNS cord-329245-6tj2k1yn 245 3 3 3 CD cord-329245-6tj2k1yn 245 4 were be VBD cord-329245-6tj2k1yn 245 5 constructed construct VBN cord-329245-6tj2k1yn 245 6 by by IN cord-329245-6tj2k1yn 245 7 oligonucleotide oligonucleotide RB cord-329245-6tj2k1yn 245 8 - - HYPH cord-329245-6tj2k1yn 245 9 directed direct VBN cord-329245-6tj2k1yn 245 10 mutagenesis mutagenesis NN cord-329245-6tj2k1yn 245 11 as as IN cord-329245-6tj2k1yn 245 12 described describe VBN cord-329245-6tj2k1yn 245 13 ( ( -LRB- cord-329245-6tj2k1yn 245 14 Machamer Machamer NNP cord-329245-6tj2k1yn 245 15 and and CC cord-329245-6tj2k1yn 245 16 Rose Rose NNP cord-329245-6tj2k1yn 245 17 , , , cord-329245-6tj2k1yn 245 18 1987 1987 CD cord-329245-6tj2k1yn 245 19 ) ) -RRB- cord-329245-6tj2k1yn 245 20 . . . cord-329245-6tj2k1yn 246 1 Mct Mct NNP cord-329245-6tj2k1yn 246 2 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 246 3 1 1 CD cord-329245-6tj2k1yn 246 4 is be VBZ cord-329245-6tj2k1yn 246 5 missing miss VBG cord-329245-6tj2k1yn 246 6 amino amino JJ cord-329245-6tj2k1yn 246 7 acids acid NNS cord-329245-6tj2k1yn 246 8 103 103 CD cord-329245-6tj2k1yn 246 9 - - HYPH cord-329245-6tj2k1yn 246 10 203 203 CD cord-329245-6tj2k1yn 247 1 , , , cord-329245-6tj2k1yn 247 2 Mct Mct NNP cord-329245-6tj2k1yn 247 3 ⌬ ⌬ NNS cord-329245-6tj2k1yn 247 4 2 2 CD cord-329245-6tj2k1yn 247 5 is be VBZ cord-329245-6tj2k1yn 247 6 missing miss VBG cord-329245-6tj2k1yn 247 7 amino amino JJ cord-329245-6tj2k1yn 247 8 acids acid NNS cord-329245-6tj2k1yn 247 9 119 119 CD cord-329245-6tj2k1yn 247 10 -203 -203 NN cord-329245-6tj2k1yn 247 11 , , , cord-329245-6tj2k1yn 247 12 and and CC cord-329245-6tj2k1yn 247 13 Mct Mct NNP cord-329245-6tj2k1yn 247 14 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 247 15 3 3 CD cord-329245-6tj2k1yn 247 16 is be VBZ cord-329245-6tj2k1yn 247 17 missing miss VBG cord-329245-6tj2k1yn 247 18 amino amino JJ cord-329245-6tj2k1yn 247 19 acids acid NNS cord-329245-6tj2k1yn 247 20 103 103 CD cord-329245-6tj2k1yn 247 21 - - SYM cord-329245-6tj2k1yn 247 22 118 118 CD cord-329245-6tj2k1yn 247 23 . . . cord-329245-6tj2k1yn 248 1 All all DT cord-329245-6tj2k1yn 248 2 three three CD cord-329245-6tj2k1yn 248 3 of of IN cord-329245-6tj2k1yn 248 4 these these DT cord-329245-6tj2k1yn 248 5 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 248 6 tail tail NN cord-329245-6tj2k1yn 248 7 deletion deletion NN cord-329245-6tj2k1yn 248 8 mutants mutant NNS cord-329245-6tj2k1yn 248 9 contain contain VBP cord-329245-6tj2k1yn 248 10 the the DT cord-329245-6tj2k1yn 248 11 IBV IBV NNP cord-329245-6tj2k1yn 248 12 M M NNP cord-329245-6tj2k1yn 248 13 C C NNP cord-329245-6tj2k1yn 248 14 - - HYPH cord-329245-6tj2k1yn 248 15 terminal terminal NN cord-329245-6tj2k1yn 248 16 22 22 CD cord-329245-6tj2k1yn 248 17 amino amino JJ cord-329245-6tj2k1yn 248 18 acids acid NNS cord-329245-6tj2k1yn 248 19 that that WDT cord-329245-6tj2k1yn 248 20 are be VBP cord-329245-6tj2k1yn 248 21 recognized recognize VBN cord-329245-6tj2k1yn 248 22 by by IN cord-329245-6tj2k1yn 248 23 the the DT cord-329245-6tj2k1yn 248 24 polyclonal polyclonal JJ cord-329245-6tj2k1yn 248 25 anti anti NNP cord-329245-6tj2k1yn 248 26 - - JJ cord-329245-6tj2k1yn 248 27 M m JJ cord-329245-6tj2k1yn 248 28 antibody antibody NN cord-329245-6tj2k1yn 248 29 ( ( -LRB- cord-329245-6tj2k1yn 248 30 see see VB cord-329245-6tj2k1yn 248 31 Fig Fig NNP cord-329245-6tj2k1yn 248 32 . . . cord-329245-6tj2k1yn 248 33 3B 3B NNP cord-329245-6tj2k1yn 248 34 ) ) -RRB- cord-329245-6tj2k1yn 248 35 . . . cord-329245-6tj2k1yn 249 1 pAR pAR NNP cord-329245-6tj2k1yn 250 1 /E /E NNS cord-329245-6tj2k1yn 250 2 and and CC cord-329245-6tj2k1yn 250 3 pAR pAR NNP cord-329245-6tj2k1yn 250 4 / / SYM cord-329245-6tj2k1yn 250 5 CTE CTE NNP cord-329245-6tj2k1yn 250 6 were be VBD cord-329245-6tj2k1yn 250 7 made make VBN cord-329245-6tj2k1yn 250 8 by by IN cord-329245-6tj2k1yn 250 9 PCR PCR NNP cord-329245-6tj2k1yn 250 10 addition addition NN cord-329245-6tj2k1yn 250 11 of of IN cord-329245-6tj2k1yn 250 12 BamHI bamhi NN cord-329245-6tj2k1yn 250 13 sites site NNS cord-329245-6tj2k1yn 250 14 at at IN cord-329245-6tj2k1yn 250 15 both both DT cord-329245-6tj2k1yn 250 16 ends end NNS cord-329245-6tj2k1yn 250 17 of of IN cord-329245-6tj2k1yn 250 18 the the DT cord-329245-6tj2k1yn 250 19 E E NNP cord-329245-6tj2k1yn 250 20 and and CC cord-329245-6tj2k1yn 250 21 CTE CTE NNP cord-329245-6tj2k1yn 250 22 inserts insert NNS cord-329245-6tj2k1yn 250 23 . . . cord-329245-6tj2k1yn 251 1 pBS pBS NNP cord-329245-6tj2k1yn 251 2 / / SYM cord-329245-6tj2k1yn 251 3 IBV IBV NNP cord-329245-6tj2k1yn 251 4 E E NNP cord-329245-6tj2k1yn 251 5 and and CC cord-329245-6tj2k1yn 251 6 pBS pbs JJ cord-329245-6tj2k1yn 251 7 / / SYM cord-329245-6tj2k1yn 251 8 CTE CTE NNP cord-329245-6tj2k1yn 251 9 ( ( -LRB- cord-329245-6tj2k1yn 251 10 Corse Corse NNP cord-329245-6tj2k1yn 251 11 and and CC cord-329245-6tj2k1yn 251 12 Machamer Machamer NNP cord-329245-6tj2k1yn 251 13 , , , cord-329245-6tj2k1yn 251 14 2002 2002 CD cord-329245-6tj2k1yn 251 15 ) ) -RRB- cord-329245-6tj2k1yn 251 16 , , , cord-329245-6tj2k1yn 251 17 respectively respectively RB cord-329245-6tj2k1yn 251 18 , , , cord-329245-6tj2k1yn 251 19 were be VBD cord-329245-6tj2k1yn 251 20 used use VBN cord-329245-6tj2k1yn 251 21 as as IN cord-329245-6tj2k1yn 251 22 templates template NNS cord-329245-6tj2k1yn 251 23 for for IN cord-329245-6tj2k1yn 251 24 PCR PCR NNP cord-329245-6tj2k1yn 251 25 . . . cord-329245-6tj2k1yn 252 1 The the DT cord-329245-6tj2k1yn 252 2 BamHI bamhi NN cord-329245-6tj2k1yn 252 3 - - HYPH cord-329245-6tj2k1yn 252 4 digested digest VBN cord-329245-6tj2k1yn 252 5 PCR PCR NNP cord-329245-6tj2k1yn 252 6 products product NNS cord-329245-6tj2k1yn 252 7 were be VBD cord-329245-6tj2k1yn 252 8 cloned clone VBN cord-329245-6tj2k1yn 252 9 into into IN cord-329245-6tj2k1yn 252 10 the the DT cord-329245-6tj2k1yn 252 11 BamHI BamHI NNP cord-329245-6tj2k1yn 252 12 site site NN cord-329245-6tj2k1yn 252 13 of of IN cord-329245-6tj2k1yn 252 14 pAR2529 pAR2529 NNP cord-329245-6tj2k1yn 252 15 , , , cord-329245-6tj2k1yn 252 16 and and CC cord-329245-6tj2k1yn 252 17 subclones subclone NNS cord-329245-6tj2k1yn 252 18 were be VBD cord-329245-6tj2k1yn 252 19 screened screen VBN cord-329245-6tj2k1yn 252 20 for for IN cord-329245-6tj2k1yn 252 21 correct correct JJ cord-329245-6tj2k1yn 252 22 orientation orientation NN cord-329245-6tj2k1yn 252 23 by by IN cord-329245-6tj2k1yn 252 24 indirect indirect JJ cord-329245-6tj2k1yn 252 25 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 252 26 . . . cord-329245-6tj2k1yn 253 1 The the DT cord-329245-6tj2k1yn 253 2 pT7/ pt7/ NN cord-329245-6tj2k1yn 254 1 Mc Mc NNP cord-329245-6tj2k1yn 254 2 ⌬ ⌬ NNP cord-329245-6tj2k1yn 255 1 1 1 LS cord-329245-6tj2k1yn 255 2 , , , cord-329245-6tj2k1yn 255 3 pT7/ pt7/ NN cord-329245-6tj2k1yn 256 1 Mct Mct NNP cord-329245-6tj2k1yn 256 2 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 256 3 2 2 CD cord-329245-6tj2k1yn 256 4 , , , cord-329245-6tj2k1yn 256 5 and and CC cord-329245-6tj2k1yn 256 6 pT7/ pt7/ NN cord-329245-6tj2k1yn 257 1 Mct Mct NNP cord-329245-6tj2k1yn 257 2 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 257 3 3 3 CD cord-329245-6tj2k1yn 257 4 plasmids plasmid NNS cord-329245-6tj2k1yn 257 5 used use VBN cord-329245-6tj2k1yn 257 6 in in IN cord-329245-6tj2k1yn 257 7 VLP VLP NNP cord-329245-6tj2k1yn 257 8 experiments experiment NNS cord-329245-6tj2k1yn 257 9 were be VBD cord-329245-6tj2k1yn 257 10 made make VBN cord-329245-6tj2k1yn 257 11 by by IN cord-329245-6tj2k1yn 257 12 PCR PCR NNP cord-329245-6tj2k1yn 257 13 addition addition NN cord-329245-6tj2k1yn 257 14 of of IN cord-329245-6tj2k1yn 257 15 EcoRI EcoRI NNP cord-329245-6tj2k1yn 257 16 and and CC cord-329245-6tj2k1yn 257 17 XbaI XbaI NNP cord-329245-6tj2k1yn 257 18 restriction restriction NN cord-329245-6tj2k1yn 257 19 sites site NNS cord-329245-6tj2k1yn 257 20 , , , cord-329245-6tj2k1yn 257 21 with with IN cord-329245-6tj2k1yn 257 22 the the DT cord-329245-6tj2k1yn 257 23 corresponding correspond VBG cord-329245-6tj2k1yn 257 24 AR AR NNP cord-329245-6tj2k1yn 257 25 plasmids plasmid NNS cord-329245-6tj2k1yn 257 26 as as IN cord-329245-6tj2k1yn 257 27 templates template NNS cord-329245-6tj2k1yn 257 28 . . . cord-329245-6tj2k1yn 258 1 The the DT cord-329245-6tj2k1yn 258 2 T7/ T7/ NNP cord-329245-6tj2k1yn 258 3 M M NNP cord-329245-6tj2k1yn 258 4 ⌬ ⌬ NNP cord-329245-6tj2k1yn 258 5 m2,3 m2,3 NNP cord-329245-6tj2k1yn 258 6 plasmid plasmid NN cord-329245-6tj2k1yn 258 7 used use VBN cord-329245-6tj2k1yn 258 8 in in IN cord-329245-6tj2k1yn 258 9 VLP VLP NNP cord-329245-6tj2k1yn 258 10 experiments experiment NNS cord-329245-6tj2k1yn 258 11 was be VBD cord-329245-6tj2k1yn 258 12 made make VBN cord-329245-6tj2k1yn 258 13 by by IN cord-329245-6tj2k1yn 258 14 subcloning subclone VBG cord-329245-6tj2k1yn 258 15 the the DT cord-329245-6tj2k1yn 258 16 pAR pAR NNP cord-329245-6tj2k1yn 258 17 / / SYM cord-329245-6tj2k1yn 258 18 M M NNP cord-329245-6tj2k1yn 258 19 ⌬ ⌬ NNPS cord-329245-6tj2k1yn 259 1 m2,3 m2,3 NNP cord-329245-6tj2k1yn 259 2 BamHI BamHI NNP cord-329245-6tj2k1yn 259 3 fragment fragment NN cord-329245-6tj2k1yn 259 4 into into IN cord-329245-6tj2k1yn 259 5 the the DT cord-329245-6tj2k1yn 259 6 the the DT cord-329245-6tj2k1yn 259 7 BamHI BamHI NNP cord-329245-6tj2k1yn 259 8 site site NN cord-329245-6tj2k1yn 259 9 of of IN cord-329245-6tj2k1yn 259 10 pT7 pT7 NNP cord-329245-6tj2k1yn 259 11 / / SYM cord-329245-6tj2k1yn 259 12 T3 T3 NNP cord-329245-6tj2k1yn 259 13 - - HYPH cord-329245-6tj2k1yn 259 14 18 18 CD cord-329245-6tj2k1yn 259 15 . . . cord-329245-6tj2k1yn 260 1 The the DT cord-329245-6tj2k1yn 260 2 polyclonal polyclonal JJ cord-329245-6tj2k1yn 260 3 anti anti JJ cord-329245-6tj2k1yn 260 4 - - JJ cord-329245-6tj2k1yn 260 5 M m JJ cord-329245-6tj2k1yn 260 6 antibody antibody NN cord-329245-6tj2k1yn 260 7 and and CC cord-329245-6tj2k1yn 260 8 its -PRON- PRP$ cord-329245-6tj2k1yn 260 9 affinity affinity NN cord-329245-6tj2k1yn 260 10 purification purification NN cord-329245-6tj2k1yn 260 11 for for IN cord-329245-6tj2k1yn 260 12 use use NN cord-329245-6tj2k1yn 260 13 in in IN cord-329245-6tj2k1yn 260 14 immunofluorescence immunofluorescence NN cord-329245-6tj2k1yn 260 15 has have VBZ cord-329245-6tj2k1yn 260 16 been be VBN cord-329245-6tj2k1yn 260 17 previously previously RB cord-329245-6tj2k1yn 260 18 described describe VBN cord-329245-6tj2k1yn 260 19 ( ( -LRB- cord-329245-6tj2k1yn 260 20 Machamer Machamer NNP cord-329245-6tj2k1yn 260 21 and and CC cord-329245-6tj2k1yn 260 22 Rose Rose NNP cord-329245-6tj2k1yn 260 23 , , , cord-329245-6tj2k1yn 260 24 1987 1987 CD cord-329245-6tj2k1yn 260 25 ) ) -RRB- cord-329245-6tj2k1yn 260 26 . . . cord-329245-6tj2k1yn 261 1 The the DT cord-329245-6tj2k1yn 261 2 rat rat NN cord-329245-6tj2k1yn 261 3 and and CC cord-329245-6tj2k1yn 261 4 rabbit rabbit NN cord-329245-6tj2k1yn 261 5 polyclonal polyclonal JJ cord-329245-6tj2k1yn 261 6 antibodies antibody NNS cord-329245-6tj2k1yn 261 7 to to IN cord-329245-6tj2k1yn 261 8 the the DT cord-329245-6tj2k1yn 261 9 C C NNP cord-329245-6tj2k1yn 261 10 - - HYPH cord-329245-6tj2k1yn 261 11 terminal terminal NN cord-329245-6tj2k1yn 261 12 14 14 CD cord-329245-6tj2k1yn 261 13 amino amino JJ cord-329245-6tj2k1yn 261 14 acids acid NNS cord-329245-6tj2k1yn 261 15 of of IN cord-329245-6tj2k1yn 261 16 IBV IBV NNP cord-329245-6tj2k1yn 261 17 E E NNP cord-329245-6tj2k1yn 261 18 have have VBP cord-329245-6tj2k1yn 261 19 been be VBN cord-329245-6tj2k1yn 261 20 reported report VBN cord-329245-6tj2k1yn 261 21 ( ( -LRB- cord-329245-6tj2k1yn 261 22 Corse Corse NNP cord-329245-6tj2k1yn 261 23 and and CC cord-329245-6tj2k1yn 261 24 Machamer Machamer NNP cord-329245-6tj2k1yn 261 25 , , , cord-329245-6tj2k1yn 261 26 2000 2000 CD cord-329245-6tj2k1yn 261 27 ) ) -RRB- cord-329245-6tj2k1yn 261 28 . . . cord-329245-6tj2k1yn 262 1 The the DT cord-329245-6tj2k1yn 262 2 rabbit rabbit NN cord-329245-6tj2k1yn 262 3 anti anti JJ cord-329245-6tj2k1yn 262 4 - - JJ cord-329245-6tj2k1yn 262 5 VSV VSV NNP cord-329245-6tj2k1yn 262 6 polyclonal polyclonal JJ cord-329245-6tj2k1yn 262 7 antibody antibody NN cord-329245-6tj2k1yn 262 8 used use VBD cord-329245-6tj2k1yn 262 9 to to TO cord-329245-6tj2k1yn 262 10 immunoprecipitate immunoprecipitate NNP cord-329245-6tj2k1yn 262 11 radiolabeled radiolabele VBD cord-329245-6tj2k1yn 262 12 G G NNP cord-329245-6tj2k1yn 262 13 and and CC cord-329245-6tj2k1yn 262 14 GEt get VBP cord-329245-6tj2k1yn 262 15 proteins protein NNS cord-329245-6tj2k1yn 262 16 has have VBZ cord-329245-6tj2k1yn 262 17 been be VBN cord-329245-6tj2k1yn 262 18 described describe VBN cord-329245-6tj2k1yn 262 19 ( ( -LRB- cord-329245-6tj2k1yn 262 20 Weisz Weisz NNP cord-329245-6tj2k1yn 262 21 et et NNP cord-329245-6tj2k1yn 262 22 al al NNP cord-329245-6tj2k1yn 262 23 . . NNP cord-329245-6tj2k1yn 262 24 , , , cord-329245-6tj2k1yn 262 25 1993 1993 CD cord-329245-6tj2k1yn 262 26 ) ) -RRB- cord-329245-6tj2k1yn 262 27 . . . cord-329245-6tj2k1yn 263 1 Texas Texas NNP cord-329245-6tj2k1yn 263 2 red red JJ cord-329245-6tj2k1yn 263 3 conjugated conjugated JJ cord-329245-6tj2k1yn 263 4 donkey donkey NNP cord-329245-6tj2k1yn 263 5 anti anti JJ cord-329245-6tj2k1yn 263 6 - - JJ cord-329245-6tj2k1yn 263 7 rabbit rabbit JJ cord-329245-6tj2k1yn 263 8 immunoglobulin immunoglobulin NN cord-329245-6tj2k1yn 263 9 G G NNP cord-329245-6tj2k1yn 263 10 ( ( -LRB- cord-329245-6tj2k1yn 263 11 IgG IgG NNP cord-329245-6tj2k1yn 263 12 ) ) -RRB- cord-329245-6tj2k1yn 263 13 and and CC cord-329245-6tj2k1yn 263 14 fluorescein fluorescein NN cord-329245-6tj2k1yn 263 15 - - HYPH cord-329245-6tj2k1yn 263 16 conjugated conjugate VBN cord-329245-6tj2k1yn 263 17 goat goat NN cord-329245-6tj2k1yn 263 18 anti anti JJ cord-329245-6tj2k1yn 263 19 - - JJ cord-329245-6tj2k1yn 263 20 rat rat JJ cord-329245-6tj2k1yn 263 21 IgG igg NN cord-329245-6tj2k1yn 263 22 were be VBD cord-329245-6tj2k1yn 263 23 from from IN cord-329245-6tj2k1yn 263 24 Jackson Jackson NNP cord-329245-6tj2k1yn 263 25 ImmunoResearch ImmunoResearch NNP cord-329245-6tj2k1yn 263 26 Laboratories Laboratories NNP cord-329245-6tj2k1yn 263 27 , , , cord-329245-6tj2k1yn 263 28 Inc. Inc. NNP cord-329245-6tj2k1yn 263 29 ( ( -LRB- cord-329245-6tj2k1yn 263 30 West West NNP cord-329245-6tj2k1yn 263 31 Grove Grove NNP cord-329245-6tj2k1yn 263 32 , , , cord-329245-6tj2k1yn 263 33 PA PA NNP cord-329245-6tj2k1yn 263 34 ) ) -RRB- cord-329245-6tj2k1yn 263 35 . . . cord-329245-6tj2k1yn 264 1 Vero Vero NNP cord-329245-6tj2k1yn 264 2 cells cell NNS cord-329245-6tj2k1yn 264 3 were be VBD cord-329245-6tj2k1yn 264 4 plated plate VBN cord-329245-6tj2k1yn 264 5 in in IN cord-329245-6tj2k1yn 264 6 35-mm 35-mm CD cord-329245-6tj2k1yn 264 7 dishes dish NNS cord-329245-6tj2k1yn 264 8 1 1 CD cord-329245-6tj2k1yn 264 9 day day NN cord-329245-6tj2k1yn 264 10 before before IN cord-329245-6tj2k1yn 264 11 being be VBG cord-329245-6tj2k1yn 264 12 infected infect VBN cord-329245-6tj2k1yn 264 13 with with IN cord-329245-6tj2k1yn 264 14 IBV IBV NNP cord-329245-6tj2k1yn 264 15 ( ( -LRB- cord-329245-6tj2k1yn 264 16 passage passage NN cord-329245-6tj2k1yn 264 17 12 12 CD cord-329245-6tj2k1yn 264 18 ) ) -RRB- cord-329245-6tj2k1yn 264 19 , , , cord-329245-6tj2k1yn 264 20 as as IN cord-329245-6tj2k1yn 264 21 previously previously RB cord-329245-6tj2k1yn 264 22 described describe VBN cord-329245-6tj2k1yn 264 23 ( ( -LRB- cord-329245-6tj2k1yn 264 24 Machamer Machamer NNP cord-329245-6tj2k1yn 264 25 and and CC cord-329245-6tj2k1yn 264 26 Rose Rose NNP cord-329245-6tj2k1yn 264 27 , , , cord-329245-6tj2k1yn 264 28 1987 1987 CD cord-329245-6tj2k1yn 264 29 ) ) -RRB- cord-329245-6tj2k1yn 264 30 . . . cord-329245-6tj2k1yn 265 1 The the DT cord-329245-6tj2k1yn 265 2 cells cell NNS cord-329245-6tj2k1yn 265 3 were be VBD cord-329245-6tj2k1yn 265 4 fixed fix VBN cord-329245-6tj2k1yn 265 5 in in IN cord-329245-6tj2k1yn 265 6 3 3 CD cord-329245-6tj2k1yn 265 7 % % NN cord-329245-6tj2k1yn 265 8 paraformaldehyde paraformaldehyde NN cord-329245-6tj2k1yn 265 9 in in IN cord-329245-6tj2k1yn 265 10 phosphate phosphate NN cord-329245-6tj2k1yn 265 11 - - HYPH cord-329245-6tj2k1yn 265 12 buffered buffer VBN cord-329245-6tj2k1yn 265 13 saline saline NN cord-329245-6tj2k1yn 265 14 ( ( -LRB- cord-329245-6tj2k1yn 265 15 PBS PBS NNP cord-329245-6tj2k1yn 265 16 ) ) -RRB- cord-329245-6tj2k1yn 265 17 for for IN cord-329245-6tj2k1yn 265 18 20 20 CD cord-329245-6tj2k1yn 265 19 min min NN cord-329245-6tj2k1yn 265 20 at at IN cord-329245-6tj2k1yn 265 21 room room NN cord-329245-6tj2k1yn 265 22 temperature temperature NN cord-329245-6tj2k1yn 265 23 , , , cord-329245-6tj2k1yn 265 24 permeabilized permeabilize VBN cord-329245-6tj2k1yn 265 25 with with IN cord-329245-6tj2k1yn 265 26 0.5 0.5 CD cord-329245-6tj2k1yn 265 27 % % NN cord-329245-6tj2k1yn 265 28 Triton Triton NNP cord-329245-6tj2k1yn 265 29 X-100 x-100 NN cord-329245-6tj2k1yn 265 30 , , , cord-329245-6tj2k1yn 265 31 and and CC cord-329245-6tj2k1yn 265 32 stained stain VBD cord-329245-6tj2k1yn 265 33 as as IN cord-329245-6tj2k1yn 265 34 previously previously RB cord-329245-6tj2k1yn 265 35 described describe VBN cord-329245-6tj2k1yn 265 36 ( ( -LRB- cord-329245-6tj2k1yn 265 37 Swift Swift NNP cord-329245-6tj2k1yn 265 38 and and CC cord-329245-6tj2k1yn 265 39 Machamer Machamer NNP cord-329245-6tj2k1yn 265 40 , , , cord-329245-6tj2k1yn 265 41 1991 1991 CD cord-329245-6tj2k1yn 265 42 ) ) -RRB- cord-329245-6tj2k1yn 265 43 with with IN cord-329245-6tj2k1yn 265 44 rat rat NNP cord-329245-6tj2k1yn 265 45 anti anti JJ cord-329245-6tj2k1yn 265 46 - - JJ cord-329245-6tj2k1yn 265 47 E e JJ cord-329245-6tj2k1yn 265 48 antibody antibody NN cord-329245-6tj2k1yn 265 49 and and CC cord-329245-6tj2k1yn 265 50 rabbit rabbit NN cord-329245-6tj2k1yn 265 51 anti anti NNP cord-329245-6tj2k1yn 265 52 - - JJ cord-329245-6tj2k1yn 265 53 M M NNP cord-329245-6tj2k1yn 265 54 antibody antibody NN cord-329245-6tj2k1yn 265 55 . . . cord-329245-6tj2k1yn 266 1 OST7 OST7 NNP cord-329245-6tj2k1yn 266 2 - - HYPH cord-329245-6tj2k1yn 266 3 1 1 CD cord-329245-6tj2k1yn 266 4 cells cell NNS cord-329245-6tj2k1yn 266 5 were be VBD cord-329245-6tj2k1yn 266 6 plated plate VBN cord-329245-6tj2k1yn 266 7 on on IN cord-329245-6tj2k1yn 266 8 cover cover NN cord-329245-6tj2k1yn 266 9 slips slip NNS cord-329245-6tj2k1yn 266 10 in in IN cord-329245-6tj2k1yn 266 11 35-mm 35-mm CD cord-329245-6tj2k1yn 266 12 dishes dish NNS cord-329245-6tj2k1yn 266 13 2 2 CD cord-329245-6tj2k1yn 266 14 days day NNS cord-329245-6tj2k1yn 266 15 before before IN cord-329245-6tj2k1yn 266 16 infection infection NN cord-329245-6tj2k1yn 266 17 with with IN cord-329245-6tj2k1yn 266 18 vTF7 vTF7 NNP cord-329245-6tj2k1yn 266 19 - - HYPH cord-329245-6tj2k1yn 266 20 3 3 CD cord-329245-6tj2k1yn 266 21 , , , cord-329245-6tj2k1yn 266 22 vvIBVE vvibve UH cord-329245-6tj2k1yn 266 23 , , , cord-329245-6tj2k1yn 266 24 vvEG3 vvEG3 NNP cord-329245-6tj2k1yn 266 25 , , , cord-329245-6tj2k1yn 266 26 or or CC cord-329245-6tj2k1yn 266 27 vvCTE vvCTE VBN cord-329245-6tj2k1yn 266 28 at at IN cord-329245-6tj2k1yn 266 29 a a DT cord-329245-6tj2k1yn 266 30 multiplicity multiplicity NN cord-329245-6tj2k1yn 266 31 of of IN cord-329245-6tj2k1yn 266 32 infection infection NN cord-329245-6tj2k1yn 266 33 of of IN cord-329245-6tj2k1yn 266 34 20 20 CD cord-329245-6tj2k1yn 266 35 . . . cord-329245-6tj2k1yn 267 1 Adsorption Adsorption NNP cord-329245-6tj2k1yn 267 2 was be VBD cord-329245-6tj2k1yn 267 3 for for IN cord-329245-6tj2k1yn 267 4 1 1 CD cord-329245-6tj2k1yn 267 5 h h NN cord-329245-6tj2k1yn 267 6 at at IN cord-329245-6tj2k1yn 267 7 37 37 CD cord-329245-6tj2k1yn 267 8 ° ° NN cord-329245-6tj2k1yn 267 9 C c NN cord-329245-6tj2k1yn 267 10 , , , cord-329245-6tj2k1yn 267 11 and and CC cord-329245-6tj2k1yn 267 12 cells cell NNS cord-329245-6tj2k1yn 267 13 infected infect VBN cord-329245-6tj2k1yn 267 14 with with IN cord-329245-6tj2k1yn 267 15 vTF7 vTF7 NNP cord-329245-6tj2k1yn 267 16 - - HYPH cord-329245-6tj2k1yn 267 17 3 3 CD cord-329245-6tj2k1yn 267 18 were be VBD cord-329245-6tj2k1yn 267 19 transfected transfecte VBN cord-329245-6tj2k1yn 267 20 with with IN cord-329245-6tj2k1yn 267 21 5 5 CD cord-329245-6tj2k1yn 267 22 g g NN cord-329245-6tj2k1yn 267 23 of of IN cord-329245-6tj2k1yn 267 24 each each DT cord-329245-6tj2k1yn 267 25 expression expression NN cord-329245-6tj2k1yn 267 26 plasmid plasmid NN cord-329245-6tj2k1yn 267 27 using use VBG cord-329245-6tj2k1yn 267 28 20 20 CD cord-329245-6tj2k1yn 267 29 l l NN cord-329245-6tj2k1yn 267 30 of of IN cord-329245-6tj2k1yn 267 31 Lipofectin Lipofectin NNP cord-329245-6tj2k1yn 267 32 ( ( -LRB- cord-329245-6tj2k1yn 267 33 Invitrogen Invitrogen NNP cord-329245-6tj2k1yn 267 34 , , , cord-329245-6tj2k1yn 267 35 Carlsbad Carlsbad NNP cord-329245-6tj2k1yn 267 36 , , , cord-329245-6tj2k1yn 267 37 CA CA NNP cord-329245-6tj2k1yn 267 38 ) ) -RRB- cord-329245-6tj2k1yn 267 39 as as IN cord-329245-6tj2k1yn 267 40 directed direct VBN cord-329245-6tj2k1yn 267 41 by by IN cord-329245-6tj2k1yn 267 42 the the DT cord-329245-6tj2k1yn 267 43 manufacturer manufacturer NN cord-329245-6tj2k1yn 267 44 . . . cord-329245-6tj2k1yn 268 1 At at IN cord-329245-6tj2k1yn 268 2 3.5 3.5 CD cord-329245-6tj2k1yn 268 3 to to IN cord-329245-6tj2k1yn 268 4 4 4 CD cord-329245-6tj2k1yn 268 5 h h NN cord-329245-6tj2k1yn 268 6 postinfection postinfection NN cord-329245-6tj2k1yn 268 7 the the DT cord-329245-6tj2k1yn 268 8 cells cell NNS cord-329245-6tj2k1yn 268 9 were be VBD cord-329245-6tj2k1yn 268 10 fixed fix VBN cord-329245-6tj2k1yn 268 11 and and CC cord-329245-6tj2k1yn 268 12 stained stain VBN cord-329245-6tj2k1yn 268 13 as as IN cord-329245-6tj2k1yn 268 14 described describe VBN cord-329245-6tj2k1yn 268 15 above above RB cord-329245-6tj2k1yn 268 16 . . . cord-329245-6tj2k1yn 269 1 OST7 OST7 NNP cord-329245-6tj2k1yn 269 2 - - HYPH cord-329245-6tj2k1yn 269 3 1 1 CD cord-329245-6tj2k1yn 269 4 cells cell NNS cord-329245-6tj2k1yn 269 5 were be VBD cord-329245-6tj2k1yn 269 6 plated plate VBN cord-329245-6tj2k1yn 269 7 in in IN cord-329245-6tj2k1yn 269 8 35-mm 35-mm CD cord-329245-6tj2k1yn 269 9 dishes dish NNS cord-329245-6tj2k1yn 269 10 2 2 CD cord-329245-6tj2k1yn 269 11 days day NNS cord-329245-6tj2k1yn 269 12 before before IN cord-329245-6tj2k1yn 269 13 being be VBG cord-329245-6tj2k1yn 269 14 infected infect VBN cord-329245-6tj2k1yn 269 15 with with IN cord-329245-6tj2k1yn 269 16 vTF7 vTF7 NNP cord-329245-6tj2k1yn 269 17 - - HYPH cord-329245-6tj2k1yn 269 18 3 3 CD cord-329245-6tj2k1yn 269 19 and and CC cord-329245-6tj2k1yn 269 20 transfected transfecte VBN cord-329245-6tj2k1yn 269 21 with with IN cord-329245-6tj2k1yn 269 22 the the DT cord-329245-6tj2k1yn 269 23 appropriate appropriate JJ cord-329245-6tj2k1yn 269 24 constructs construct NNS cord-329245-6tj2k1yn 269 25 as as IN cord-329245-6tj2k1yn 269 26 described describe VBN cord-329245-6tj2k1yn 269 27 above above RB cord-329245-6tj2k1yn 269 28 . . . cord-329245-6tj2k1yn 270 1 In in IN cord-329245-6tj2k1yn 270 2 samples sample NNS cord-329245-6tj2k1yn 270 3 transfected transfecte VBN cord-329245-6tj2k1yn 270 4 with with IN cord-329245-6tj2k1yn 270 5 only only RB cord-329245-6tj2k1yn 270 6 a a DT cord-329245-6tj2k1yn 270 7 plasmid plasmid NN cord-329245-6tj2k1yn 270 8 encoding encode VBG cord-329245-6tj2k1yn 270 9 IBV IBV NNP cord-329245-6tj2k1yn 270 10 E E NNP cord-329245-6tj2k1yn 270 11 or or CC cord-329245-6tj2k1yn 270 12 IBV IBV NNP cord-329245-6tj2k1yn 270 13 M M NNP cord-329245-6tj2k1yn 270 14 alone alone RB cord-329245-6tj2k1yn 270 15 , , , cord-329245-6tj2k1yn 270 16 an an DT cord-329245-6tj2k1yn 270 17 equal equal JJ cord-329245-6tj2k1yn 270 18 amount amount NN cord-329245-6tj2k1yn 270 19 of of IN cord-329245-6tj2k1yn 270 20 empty empty JJ cord-329245-6tj2k1yn 270 21 vector vector NN cord-329245-6tj2k1yn 270 22 plasmid plasmid NN cord-329245-6tj2k1yn 270 23 was be VBD cord-329245-6tj2k1yn 270 24 added add VBN cord-329245-6tj2k1yn 270 25 so so IN cord-329245-6tj2k1yn 270 26 that that IN cord-329245-6tj2k1yn 270 27 an an DT cord-329245-6tj2k1yn 270 28 equal equal JJ cord-329245-6tj2k1yn 270 29 amount amount NN cord-329245-6tj2k1yn 270 30 of of IN cord-329245-6tj2k1yn 270 31 plasmid plasmid NN cord-329245-6tj2k1yn 270 32 DNA DNA NNP cord-329245-6tj2k1yn 270 33 was be VBD cord-329245-6tj2k1yn 270 34 added add VBN cord-329245-6tj2k1yn 270 35 to to IN cord-329245-6tj2k1yn 270 36 all all DT cord-329245-6tj2k1yn 270 37 samples sample NNS cord-329245-6tj2k1yn 270 38 . . . cord-329245-6tj2k1yn 271 1 At at IN cord-329245-6tj2k1yn 271 2 4 4 CD cord-329245-6tj2k1yn 271 3 h h NN cord-329245-6tj2k1yn 271 4 postinfection postinfection NN cord-329245-6tj2k1yn 271 5 , , , cord-329245-6tj2k1yn 271 6 the the DT cord-329245-6tj2k1yn 271 7 cells cell NNS cord-329245-6tj2k1yn 271 8 were be VBD cord-329245-6tj2k1yn 271 9 labeled label VBN cord-329245-6tj2k1yn 271 10 with with IN cord-329245-6tj2k1yn 271 11 100 100 CD cord-329245-6tj2k1yn 271 12 Ci ci NN cord-329245-6tj2k1yn 271 13 of of IN cord-329245-6tj2k1yn 271 14 35 35 CD cord-329245-6tj2k1yn 271 15 S S NNP cord-329245-6tj2k1yn 271 16 - - HYPH cord-329245-6tj2k1yn 271 17 Promix Promix NNP cord-329245-6tj2k1yn 271 18 ( ( -LRB- cord-329245-6tj2k1yn 271 19 Amersham Amersham NNP cord-329245-6tj2k1yn 271 20 Pharmacia Pharmacia NNP cord-329245-6tj2k1yn 271 21 Biotech Biotech NNP cord-329245-6tj2k1yn 271 22 , , , cord-329245-6tj2k1yn 271 23 Piscataway Piscataway NNP cord-329245-6tj2k1yn 271 24 , , , cord-329245-6tj2k1yn 271 25 NJ NJ NNP cord-329245-6tj2k1yn 271 26 ) ) -RRB- cord-329245-6tj2k1yn 271 27 in in IN cord-329245-6tj2k1yn 271 28 0.5 0.5 CD cord-329245-6tj2k1yn 271 29 ml ml NN cord-329245-6tj2k1yn 271 30 methionine methionine NN cord-329245-6tj2k1yn 271 31 - - HYPH cord-329245-6tj2k1yn 271 32 and and CC cord-329245-6tj2k1yn 271 33 cysteine cysteine NN cord-329245-6tj2k1yn 271 34 - - HYPH cord-329245-6tj2k1yn 271 35 free free JJ cord-329245-6tj2k1yn 271 36 medium medium NN cord-329245-6tj2k1yn 271 37 for for IN cord-329245-6tj2k1yn 271 38 1 1 CD cord-329245-6tj2k1yn 271 39 to to IN cord-329245-6tj2k1yn 271 40 1.5 1.5 CD cord-329245-6tj2k1yn 271 41 h h NN cord-329245-6tj2k1yn 271 42 at at IN cord-329245-6tj2k1yn 271 43 37 37 CD cord-329245-6tj2k1yn 271 44 ° ° NN cord-329245-6tj2k1yn 271 45 C c NN cord-329245-6tj2k1yn 271 46 . . . cord-329245-6tj2k1yn 272 1 Vero Vero NNP cord-329245-6tj2k1yn 272 2 cells cell NNS cord-329245-6tj2k1yn 272 3 were be VBD cord-329245-6tj2k1yn 272 4 plated plate VBN cord-329245-6tj2k1yn 272 5 in in IN cord-329245-6tj2k1yn 272 6 a a DT cord-329245-6tj2k1yn 272 7 10-cm 10-cm CD cord-329245-6tj2k1yn 272 8 dish dish NN cord-329245-6tj2k1yn 272 9 1 1 CD cord-329245-6tj2k1yn 272 10 day day NN cord-329245-6tj2k1yn 272 11 before before IN cord-329245-6tj2k1yn 272 12 being be VBG cord-329245-6tj2k1yn 272 13 infected infect VBN cord-329245-6tj2k1yn 272 14 with with IN cord-329245-6tj2k1yn 272 15 IBV IBV NNP cord-329245-6tj2k1yn 272 16 and and CC cord-329245-6tj2k1yn 272 17 labeled label VBN cord-329245-6tj2k1yn 272 18 at at IN cord-329245-6tj2k1yn 272 19 45 45 CD cord-329245-6tj2k1yn 272 20 h h NN cord-329245-6tj2k1yn 272 21 postinfection postinfection NN cord-329245-6tj2k1yn 272 22 for for IN cord-329245-6tj2k1yn 272 23 1 1 CD cord-329245-6tj2k1yn 272 24 h h NN cord-329245-6tj2k1yn 272 25 at at IN cord-329245-6tj2k1yn 272 26 37 37 CD cord-329245-6tj2k1yn 272 27 ° ° NN cord-329245-6tj2k1yn 272 28 C c NN cord-329245-6tj2k1yn 272 29 with with IN cord-329245-6tj2k1yn 272 30 500 500 CD cord-329245-6tj2k1yn 272 31 Ci ci NN cord-329245-6tj2k1yn 272 32 of of IN cord-329245-6tj2k1yn 272 33 35 35 CD cord-329245-6tj2k1yn 272 34 S S NNP cord-329245-6tj2k1yn 272 35 - - HYPH cord-329245-6tj2k1yn 272 36 Promix Promix NNP cord-329245-6tj2k1yn 272 37 . . . cord-329245-6tj2k1yn 273 1 After after IN cord-329245-6tj2k1yn 273 2 being be VBG cord-329245-6tj2k1yn 273 3 rinsed rinse VBN cord-329245-6tj2k1yn 273 4 twice twice RB cord-329245-6tj2k1yn 273 5 in in IN cord-329245-6tj2k1yn 273 6 room room NN cord-329245-6tj2k1yn 273 7 temperature temperature NN cord-329245-6tj2k1yn 273 8 PBS PBS NNP cord-329245-6tj2k1yn 273 9 , , , cord-329245-6tj2k1yn 273 10 cells cell NNS cord-329245-6tj2k1yn 273 11 were be VBD cord-329245-6tj2k1yn 273 12 scraped scrape VBN cord-329245-6tj2k1yn 273 13 into into IN cord-329245-6tj2k1yn 273 14 PBS PBS NNP cord-329245-6tj2k1yn 273 15 , , , cord-329245-6tj2k1yn 273 16 transferred transfer VBN cord-329245-6tj2k1yn 273 17 to to IN cord-329245-6tj2k1yn 273 18 microfuge microfuge JJ cord-329245-6tj2k1yn 273 19 tubes tube NNS cord-329245-6tj2k1yn 273 20 , , , cord-329245-6tj2k1yn 273 21 and and CC cord-329245-6tj2k1yn 273 22 the the DT cord-329245-6tj2k1yn 273 23 thiol thiol NN cord-329245-6tj2k1yn 273 24 - - HYPH cord-329245-6tj2k1yn 273 25 cleavable cleavable NN cord-329245-6tj2k1yn 273 26 crosslinker crosslinker NNP cord-329245-6tj2k1yn 273 27 DSP DSP NNP cord-329245-6tj2k1yn 273 28 ( ( -LRB- cord-329245-6tj2k1yn 273 29 Pierce Pierce NNP cord-329245-6tj2k1yn 273 30 , , , cord-329245-6tj2k1yn 273 31 Rockford Rockford NNP cord-329245-6tj2k1yn 273 32 , , , cord-329245-6tj2k1yn 273 33 IL IL NNP cord-329245-6tj2k1yn 273 34 ) ) -RRB- cord-329245-6tj2k1yn 273 35 was be VBD cord-329245-6tj2k1yn 273 36 added add VBN cord-329245-6tj2k1yn 273 37 to to IN cord-329245-6tj2k1yn 273 38 1 1 CD cord-329245-6tj2k1yn 273 39 mM mm CD cord-329245-6tj2k1yn 273 40 after after IN cord-329245-6tj2k1yn 273 41 being be VBG cord-329245-6tj2k1yn 273 42 prepared prepare VBN cord-329245-6tj2k1yn 273 43 just just RB cord-329245-6tj2k1yn 273 44 before before IN cord-329245-6tj2k1yn 273 45 use use NN cord-329245-6tj2k1yn 273 46 as as IN cord-329245-6tj2k1yn 273 47 a a DT cord-329245-6tj2k1yn 273 48 20 20 CD cord-329245-6tj2k1yn 273 49 mM mm CD cord-329245-6tj2k1yn 273 50 stock stock NN cord-329245-6tj2k1yn 273 51 solution solution NN cord-329245-6tj2k1yn 273 52 in in IN cord-329245-6tj2k1yn 273 53 DMSO DMSO NNP cord-329245-6tj2k1yn 273 54 . . . cord-329245-6tj2k1yn 274 1 For for IN cord-329245-6tj2k1yn 274 2 mock mock JJ cord-329245-6tj2k1yn 274 3 DSP DSP NNP cord-329245-6tj2k1yn 274 4 treatments treatment NNS cord-329245-6tj2k1yn 274 5 an an DT cord-329245-6tj2k1yn 274 6 equivalent equivalent JJ cord-329245-6tj2k1yn 274 7 amount amount NN cord-329245-6tj2k1yn 274 8 of of IN cord-329245-6tj2k1yn 274 9 DMSO DMSO NNP cord-329245-6tj2k1yn 274 10 was be VBD cord-329245-6tj2k1yn 274 11 added add VBN cord-329245-6tj2k1yn 274 12 . . . cord-329245-6tj2k1yn 275 1 The the DT cord-329245-6tj2k1yn 275 2 cells cell NNS cord-329245-6tj2k1yn 275 3 were be VBD cord-329245-6tj2k1yn 275 4 incubated incubate VBN cord-329245-6tj2k1yn 275 5 with with IN cord-329245-6tj2k1yn 275 6 DSP DSP NNP cord-329245-6tj2k1yn 275 7 for for IN cord-329245-6tj2k1yn 275 8 10 10 CD cord-329245-6tj2k1yn 275 9 min min NN cord-329245-6tj2k1yn 275 10 at at IN cord-329245-6tj2k1yn 275 11 room room NN cord-329245-6tj2k1yn 275 12 temperature temperature NN cord-329245-6tj2k1yn 275 13 , , , cord-329245-6tj2k1yn 275 14 and and CC cord-329245-6tj2k1yn 275 15 the the DT cord-329245-6tj2k1yn 275 16 DSP DSP NNP cord-329245-6tj2k1yn 275 17 was be VBD cord-329245-6tj2k1yn 275 18 quenched quench VBN cord-329245-6tj2k1yn 275 19 by by IN cord-329245-6tj2k1yn 275 20 adding add VBG cord-329245-6tj2k1yn 275 21 glycine glycine NN cord-329245-6tj2k1yn 275 22 to to IN cord-329245-6tj2k1yn 275 23 40 40 CD cord-329245-6tj2k1yn 275 24 mM. mM. NNS cord-329245-6tj2k1yn 276 1 The the DT cord-329245-6tj2k1yn 276 2 cells cell NNS cord-329245-6tj2k1yn 276 3 were be VBD cord-329245-6tj2k1yn 276 4 lysed lyse VBN cord-329245-6tj2k1yn 276 5 in in IN cord-329245-6tj2k1yn 276 6 detergent detergent NN cord-329245-6tj2k1yn 276 7 solution solution NN cord-329245-6tj2k1yn 276 8 [ [ -LRB- cord-329245-6tj2k1yn 277 1 62.5 62.5 CD cord-329245-6tj2k1yn 277 2 mM mM NNP cord-329245-6tj2k1yn 277 3 EDTA EDTA NNP cord-329245-6tj2k1yn 277 4 , , , cord-329245-6tj2k1yn 277 5 50 50 CD cord-329245-6tj2k1yn 277 6 mM mm CD cord-329245-6tj2k1yn 277 7 Tris Tris NNP cord-329245-6tj2k1yn 277 8 ( ( -LRB- cord-329245-6tj2k1yn 277 9 pH pH NNP cord-329245-6tj2k1yn 277 10 8) 8) NNP cord-329245-6tj2k1yn 277 11 , , , cord-329245-6tj2k1yn 277 12 0.4 0.4 CD cord-329245-6tj2k1yn 277 13 % % NN cord-329245-6tj2k1yn 277 14 deoxycholate deoxycholate NN cord-329245-6tj2k1yn 277 15 , , , cord-329245-6tj2k1yn 277 16 1 1 CD cord-329245-6tj2k1yn 277 17 % % NN cord-329245-6tj2k1yn 277 18 NP-40 NP-40 NNP cord-329245-6tj2k1yn 277 19 ] ] -RRB- cord-329245-6tj2k1yn 277 20 containing contain VBG cord-329245-6tj2k1yn 277 21 protease protease NN cord-329245-6tj2k1yn 277 22 inhibitors inhibitor NNS cord-329245-6tj2k1yn 277 23 , , , cord-329245-6tj2k1yn 277 24 and and CC cord-329245-6tj2k1yn 277 25 the the DT cord-329245-6tj2k1yn 277 26 postnuclear postnuclear NNP cord-329245-6tj2k1yn 277 27 supernatants supernatants NNP cord-329245-6tj2k1yn 277 28 were be VBD cord-329245-6tj2k1yn 277 29 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 277 30 with with IN cord-329245-6tj2k1yn 277 31 the the DT cord-329245-6tj2k1yn 277 32 appropriate appropriate JJ cord-329245-6tj2k1yn 277 33 antibodies antibody NNS cord-329245-6tj2k1yn 277 34 in in IN cord-329245-6tj2k1yn 277 35 the the DT cord-329245-6tj2k1yn 277 36 presence presence NN cord-329245-6tj2k1yn 277 37 of of IN cord-329245-6tj2k1yn 277 38 0.2 0.2 CD cord-329245-6tj2k1yn 277 39 % % NN cord-329245-6tj2k1yn 277 40 SDS SDS NNP cord-329245-6tj2k1yn 277 41 as as IN cord-329245-6tj2k1yn 277 42 described describe VBN cord-329245-6tj2k1yn 277 43 ( ( -LRB- cord-329245-6tj2k1yn 277 44 Machamer Machamer NNP cord-329245-6tj2k1yn 277 45 and and CC cord-329245-6tj2k1yn 277 46 Rose Rose NNP cord-329245-6tj2k1yn 277 47 , , , cord-329245-6tj2k1yn 277 48 1987 1987 CD cord-329245-6tj2k1yn 277 49 ) ) -RRB- cord-329245-6tj2k1yn 277 50 . . . cord-329245-6tj2k1yn 278 1 Treatment treatment NN cord-329245-6tj2k1yn 278 2 of of IN cord-329245-6tj2k1yn 278 3 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 278 4 with with IN cord-329245-6tj2k1yn 278 5 N N NNP cord-329245-6tj2k1yn 278 6 - - HYPH cord-329245-6tj2k1yn 278 7 glycanase glycanase NN cord-329245-6tj2k1yn 278 8 was be VBD cord-329245-6tj2k1yn 278 9 as as RB cord-329245-6tj2k1yn 278 10 previously previously RB cord-329245-6tj2k1yn 278 11 described describe VBN cord-329245-6tj2k1yn 279 1 ( ( -LRB- cord-329245-6tj2k1yn 279 2 Machamer Machamer NNP cord-329245-6tj2k1yn 279 3 et et NNP cord-329245-6tj2k1yn 279 4 al al NNP cord-329245-6tj2k1yn 279 5 . . NNP cord-329245-6tj2k1yn 279 6 , , , cord-329245-6tj2k1yn 279 7 1990 1990 CD cord-329245-6tj2k1yn 279 8 ) ) -RRB- cord-329245-6tj2k1yn 279 9 . . . cord-329245-6tj2k1yn 280 1 The the DT cord-329245-6tj2k1yn 280 2 samples sample NNS cord-329245-6tj2k1yn 280 3 were be VBD cord-329245-6tj2k1yn 280 4 subjected subject VBN cord-329245-6tj2k1yn 280 5 to to IN cord-329245-6tj2k1yn 280 6 SDS SDS NNP cord-329245-6tj2k1yn 280 7 - - HYPH cord-329245-6tj2k1yn 280 8 PAGE PAGE NNP cord-329245-6tj2k1yn 280 9 in in IN cord-329245-6tj2k1yn 280 10 the the DT cord-329245-6tj2k1yn 280 11 presence presence NN cord-329245-6tj2k1yn 280 12 of of IN cord-329245-6tj2k1yn 280 13 ␤ ␤ NNP cord-329245-6tj2k1yn 280 14 mercaptoethanol mercaptoethanol NNP cord-329245-6tj2k1yn 280 15 ( ( -LRB- cord-329245-6tj2k1yn 280 16 except except IN cord-329245-6tj2k1yn 280 17 for for IN cord-329245-6tj2k1yn 280 18 the the DT cord-329245-6tj2k1yn 280 19 indicated indicate VBN cord-329245-6tj2k1yn 280 20 samples sample NNS cord-329245-6tj2k1yn 280 21 in in IN cord-329245-6tj2k1yn 280 22 Fig Fig NNP cord-329245-6tj2k1yn 280 23 . . . cord-329245-6tj2k1yn 280 24 1B 1B NNP cord-329245-6tj2k1yn 280 25 ) ) -RRB- cord-329245-6tj2k1yn 280 26 and and CC cord-329245-6tj2k1yn 280 27 the the DT cord-329245-6tj2k1yn 280 28 labeled label VBN cord-329245-6tj2k1yn 280 29 proteins protein NNS cord-329245-6tj2k1yn 280 30 were be VBD cord-329245-6tj2k1yn 280 31 visualized visualize VBN cord-329245-6tj2k1yn 280 32 by by IN cord-329245-6tj2k1yn 280 33 fluorography fluorography NNP cord-329245-6tj2k1yn 280 34 . . . cord-329245-6tj2k1yn 281 1 Appropriate appropriate JJ cord-329245-6tj2k1yn 281 2 constructs construct NNS cord-329245-6tj2k1yn 281 3 were be VBD cord-329245-6tj2k1yn 281 4 expressed express VBN cord-329245-6tj2k1yn 281 5 in in IN cord-329245-6tj2k1yn 281 6 OST7 OST7 NNP cord-329245-6tj2k1yn 281 7 - - HYPH cord-329245-6tj2k1yn 281 8 1 1 CD cord-329245-6tj2k1yn 281 9 cells cell NNS cord-329245-6tj2k1yn 281 10 by by IN cord-329245-6tj2k1yn 281 11 infection infection NN cord-329245-6tj2k1yn 281 12 with with IN cord-329245-6tj2k1yn 281 13 vTF7 vTF7 NNP cord-329245-6tj2k1yn 281 14 - - HYPH cord-329245-6tj2k1yn 281 15 3 3 CD cord-329245-6tj2k1yn 281 16 and and CC cord-329245-6tj2k1yn 281 17 transfection transfection NN cord-329245-6tj2k1yn 281 18 as as IN cord-329245-6tj2k1yn 281 19 described describe VBN cord-329245-6tj2k1yn 281 20 above above RB cord-329245-6tj2k1yn 281 21 . . . cord-329245-6tj2k1yn 282 1 At at IN cord-329245-6tj2k1yn 282 2 3 3 CD cord-329245-6tj2k1yn 282 3 h h NN cord-329245-6tj2k1yn 282 4 postinfection postinfection NN cord-329245-6tj2k1yn 282 5 the the DT cord-329245-6tj2k1yn 282 6 cells cell NNS cord-329245-6tj2k1yn 282 7 were be VBD cord-329245-6tj2k1yn 282 8 shifted shift VBN cord-329245-6tj2k1yn 282 9 from from IN cord-329245-6tj2k1yn 282 10 37 37 CD cord-329245-6tj2k1yn 282 11 to to TO cord-329245-6tj2k1yn 282 12 32 32 CD cord-329245-6tj2k1yn 282 13 ° ° NN cord-329245-6tj2k1yn 282 14 C c NN cord-329245-6tj2k1yn 282 15 for for IN cord-329245-6tj2k1yn 282 16 1 1 CD cord-329245-6tj2k1yn 282 17 h h NN cord-329245-6tj2k1yn 282 18 and and CC cord-329245-6tj2k1yn 282 19 then then RB cord-329245-6tj2k1yn 282 20 radiolabeled radiolabele VBD cord-329245-6tj2k1yn 282 21 at at IN cord-329245-6tj2k1yn 282 22 32 32 CD cord-329245-6tj2k1yn 282 23 ° ° NN cord-329245-6tj2k1yn 282 24 C c NN cord-329245-6tj2k1yn 282 25 with with IN cord-329245-6tj2k1yn 282 26 35 35 CD cord-329245-6tj2k1yn 282 27 S S NNP cord-329245-6tj2k1yn 282 28 - - HYPH cord-329245-6tj2k1yn 282 29 Promix Promix NNP cord-329245-6tj2k1yn 282 30 for for IN cord-329245-6tj2k1yn 282 31 1 1 CD cord-329245-6tj2k1yn 282 32 to to IN cord-329245-6tj2k1yn 282 33 1.5 1.5 CD cord-329245-6tj2k1yn 282 34 h h NN cord-329245-6tj2k1yn 282 35 as as IN cord-329245-6tj2k1yn 282 36 described describe VBN cord-329245-6tj2k1yn 282 37 above above RB cord-329245-6tj2k1yn 282 38 . . . cord-329245-6tj2k1yn 283 1 After after IN cord-329245-6tj2k1yn 283 2 labeling label VBG cord-329245-6tj2k1yn 283 3 the the DT cord-329245-6tj2k1yn 283 4 cells cell NNS cord-329245-6tj2k1yn 283 5 were be VBD cord-329245-6tj2k1yn 283 6 chased chase VBN cord-329245-6tj2k1yn 283 7 in in IN cord-329245-6tj2k1yn 283 8 1 1 CD cord-329245-6tj2k1yn 283 9 ml ml NNS cord-329245-6tj2k1yn 283 10 of of IN cord-329245-6tj2k1yn 283 11 growth growth NN cord-329245-6tj2k1yn 283 12 medium medium NN cord-329245-6tj2k1yn 283 13 for for IN cord-329245-6tj2k1yn 283 14 3 3 CD cord-329245-6tj2k1yn 283 15 h h NN cord-329245-6tj2k1yn 283 16 at at IN cord-329245-6tj2k1yn 283 17 32 32 CD cord-329245-6tj2k1yn 283 18 ° ° NN cord-329245-6tj2k1yn 283 19 C c NN cord-329245-6tj2k1yn 283 20 . . . cord-329245-6tj2k1yn 284 1 The the DT cord-329245-6tj2k1yn 284 2 medium medium NN cord-329245-6tj2k1yn 284 3 was be VBD cord-329245-6tj2k1yn 284 4 microcentrifuged microcentrifuge VBN cord-329245-6tj2k1yn 284 5 at at IN cord-329245-6tj2k1yn 284 6 14,000 14,000 CD cord-329245-6tj2k1yn 284 7 rpm rpm NN cord-329245-6tj2k1yn 284 8 for for IN cord-329245-6tj2k1yn 284 9 20 20 CD cord-329245-6tj2k1yn 284 10 min min NN cord-329245-6tj2k1yn 284 11 at at IN cord-329245-6tj2k1yn 284 12 4 4 CD cord-329245-6tj2k1yn 284 13 ° ° NN cord-329245-6tj2k1yn 284 14 C c NN cord-329245-6tj2k1yn 284 15 to to TO cord-329245-6tj2k1yn 284 16 remove remove VB cord-329245-6tj2k1yn 284 17 cellular cellular JJ cord-329245-6tj2k1yn 284 18 debris debris NN cord-329245-6tj2k1yn 284 19 , , , cord-329245-6tj2k1yn 284 20 and and CC cord-329245-6tj2k1yn 284 21 the the DT cord-329245-6tj2k1yn 284 22 supernatants supernatant NNS cord-329245-6tj2k1yn 284 23 were be VBD cord-329245-6tj2k1yn 284 24 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 284 25 with with IN cord-329245-6tj2k1yn 284 26 rat rat NNP cord-329245-6tj2k1yn 284 27 anti anti NNP cord-329245-6tj2k1yn 284 28 - - NNP cord-329245-6tj2k1yn 284 29 E e JJ cord-329245-6tj2k1yn 284 30 and and CC cord-329245-6tj2k1yn 284 31 rabbit rabbit NN cord-329245-6tj2k1yn 284 32 anti anti NNP cord-329245-6tj2k1yn 284 33 - - NNP cord-329245-6tj2k1yn 284 34 M M NNP cord-329245-6tj2k1yn 284 35 antibodies antibody NNS cord-329245-6tj2k1yn 284 36 in in IN cord-329245-6tj2k1yn 284 37 the the DT cord-329245-6tj2k1yn 284 38 presence presence NN cord-329245-6tj2k1yn 284 39 of of IN cord-329245-6tj2k1yn 284 40 1 1 CD cord-329245-6tj2k1yn 284 41 % % NN cord-329245-6tj2k1yn 284 42 Triton Triton NNP cord-329245-6tj2k1yn 284 43 X-100 x-100 NN cord-329245-6tj2k1yn 284 44 and and CC cord-329245-6tj2k1yn 284 45 0.2 0.2 CD cord-329245-6tj2k1yn 284 46 % % NN cord-329245-6tj2k1yn 284 47 SDS SDS NNP cord-329245-6tj2k1yn 284 48 . . . cord-329245-6tj2k1yn 285 1 The the DT cord-329245-6tj2k1yn 285 2 cells cell NNS cord-329245-6tj2k1yn 285 3 were be VBD cord-329245-6tj2k1yn 285 4 harvested harvest VBN cord-329245-6tj2k1yn 285 5 by by IN cord-329245-6tj2k1yn 285 6 rinsing rinse VBG cord-329245-6tj2k1yn 285 7 in in IN cord-329245-6tj2k1yn 285 8 PBS PBS NNP cord-329245-6tj2k1yn 285 9 and and CC cord-329245-6tj2k1yn 285 10 lysing lyse VBG cord-329245-6tj2k1yn 285 11 in in IN cord-329245-6tj2k1yn 285 12 detergent detergent NN cord-329245-6tj2k1yn 285 13 solution solution NN cord-329245-6tj2k1yn 285 14 . . . cord-329245-6tj2k1yn 286 1 The the DT cord-329245-6tj2k1yn 286 2 postnuclear postnuclear NN cord-329245-6tj2k1yn 286 3 supernatants supernatant NNS cord-329245-6tj2k1yn 286 4 were be VBD cord-329245-6tj2k1yn 286 5 immunoprecipitated immunoprecipitate VBN cord-329245-6tj2k1yn 286 6 with with IN cord-329245-6tj2k1yn 286 7 anti anti JJ cord-329245-6tj2k1yn 286 8 - - JJ cord-329245-6tj2k1yn 286 9 E e JJ cord-329245-6tj2k1yn 286 10 and and CC cord-329245-6tj2k1yn 286 11 anti anti JJ cord-329245-6tj2k1yn 286 12 - - JJ cord-329245-6tj2k1yn 286 13 M m JJ cord-329245-6tj2k1yn 286 14 antibodies antibody NNS cord-329245-6tj2k1yn 286 15 in in IN cord-329245-6tj2k1yn 286 16 the the DT cord-329245-6tj2k1yn 286 17 presence presence NN cord-329245-6tj2k1yn 286 18 of of IN cord-329245-6tj2k1yn 286 19 0.2 0.2 CD cord-329245-6tj2k1yn 286 20 % % NN cord-329245-6tj2k1yn 286 21 SDS SDS NNP cord-329245-6tj2k1yn 286 22 . . . cord-329245-6tj2k1yn 287 1 The the DT cord-329245-6tj2k1yn 287 2 immunoprecipitates immunoprecipitate NNS cord-329245-6tj2k1yn 287 3 were be VBD cord-329245-6tj2k1yn 287 4 treated treat VBN cord-329245-6tj2k1yn 287 5 with with IN cord-329245-6tj2k1yn 287 6 N N NNP cord-329245-6tj2k1yn 287 7 - - HYPH cord-329245-6tj2k1yn 287 8 glycanase glycanase NN cord-329245-6tj2k1yn 287 9 as as IN cord-329245-6tj2k1yn 287 10 described describe VBN cord-329245-6tj2k1yn 287 11 ( ( -LRB- cord-329245-6tj2k1yn 287 12 Machamer Machamer NNP cord-329245-6tj2k1yn 287 13 et et NNP cord-329245-6tj2k1yn 287 14 al al NNP cord-329245-6tj2k1yn 287 15 . . NNP cord-329245-6tj2k1yn 287 16 , , , cord-329245-6tj2k1yn 287 17 1990 1990 CD cord-329245-6tj2k1yn 287 18 ) ) -RRB- cord-329245-6tj2k1yn 287 19 . . . cord-329245-6tj2k1yn 288 1 Mapping map VBG cord-329245-6tj2k1yn 288 2 the the DT cord-329245-6tj2k1yn 288 3 interaction interaction NN cord-329245-6tj2k1yn 288 4 between between IN cord-329245-6tj2k1yn 288 5 GRASP65 GRASP65 NNP cord-329245-6tj2k1yn 288 6 and and CC cord-329245-6tj2k1yn 288 7 GM130 GM130 NNP cord-329245-6tj2k1yn 288 8 , , , cord-329245-6tj2k1yn 288 9 components component NNS cord-329245-6tj2k1yn 288 10 of of IN cord-329245-6tj2k1yn 288 11 a a DT cord-329245-6tj2k1yn 288 12 protein protein NN cord-329245-6tj2k1yn 288 13 complex complex NN cord-329245-6tj2k1yn 288 14 involved involve VBN cord-329245-6tj2k1yn 288 15 in in IN cord-329245-6tj2k1yn 288 16 the the DT cord-329245-6tj2k1yn 288 17 stacking stacking NN cord-329245-6tj2k1yn 288 18 of of IN cord-329245-6tj2k1yn 288 19 Golgi Golgi NNP cord-329245-6tj2k1yn 288 20 cisternae cisternae JJ cord-329245-6tj2k1yn 288 21 Coronavirus Coronavirus NNP cord-329245-6tj2k1yn 288 22 pseudoparticles pseudoparticle NNS cord-329245-6tj2k1yn 288 23 formed form VBN cord-329245-6tj2k1yn 288 24 with with IN cord-329245-6tj2k1yn 288 25 recombinant recombinant JJ cord-329245-6tj2k1yn 288 26 M M NNP cord-329245-6tj2k1yn 288 27 and and CC cord-329245-6tj2k1yn 288 28 E e NN cord-329245-6tj2k1yn 288 29 proteins protein NNS cord-329245-6tj2k1yn 288 30 induce induce VBP cord-329245-6tj2k1yn 288 31 alpha alpha NN cord-329245-6tj2k1yn 288 32 interferon interferon NN cord-329245-6tj2k1yn 288 33 synthesis synthesis NN cord-329245-6tj2k1yn 288 34 by by IN cord-329245-6tj2k1yn 288 35 leukocytes leukocyte NNS cord-329245-6tj2k1yn 288 36 Infectious infectious JJ cord-329245-6tj2k1yn 288 37 bronchitis bronchitis NN cord-329245-6tj2k1yn 288 38 virus virus NN cord-329245-6tj2k1yn 289 1 E e NN cord-329245-6tj2k1yn 289 2 protein protein NN cord-329245-6tj2k1yn 289 3 is be VBZ cord-329245-6tj2k1yn 289 4 targeted target VBN cord-329245-6tj2k1yn 289 5 to to IN cord-329245-6tj2k1yn 289 6 the the DT cord-329245-6tj2k1yn 289 7 Golgi Golgi NNP cord-329245-6tj2k1yn 289 8 complex complex NN cord-329245-6tj2k1yn 289 9 and and CC cord-329245-6tj2k1yn 289 10 directs direct VBZ cord-329245-6tj2k1yn 289 11 release release NN cord-329245-6tj2k1yn 289 12 of of IN cord-329245-6tj2k1yn 289 13 virus virus NN cord-329245-6tj2k1yn 289 14 - - HYPH cord-329245-6tj2k1yn 289 15 like like JJ cord-329245-6tj2k1yn 289 16 particles particle NNS cord-329245-6tj2k1yn 289 17 Infectious infectious JJ cord-329245-6tj2k1yn 289 18 bronchitis bronchitis NN cord-329245-6tj2k1yn 289 19 virus virus NN cord-329245-6tj2k1yn 289 20 envelope envelope NN cord-329245-6tj2k1yn 289 21 protein protein NN cord-329245-6tj2k1yn 289 22 targeting target VBG cord-329245-6tj2k1yn 289 23 : : : cord-329245-6tj2k1yn 289 24 implications implication NNS cord-329245-6tj2k1yn 289 25 for for IN cord-329245-6tj2k1yn 289 26 virus virus NN cord-329245-6tj2k1yn 289 27 assembly assembly NN cord-329245-6tj2k1yn 290 1 The the DT cord-329245-6tj2k1yn 290 2 cytoplasmic cytoplasmic JJ cord-329245-6tj2k1yn 290 3 tail tail NN cord-329245-6tj2k1yn 290 4 of of IN cord-329245-6tj2k1yn 290 5 infectious infectious JJ cord-329245-6tj2k1yn 290 6 bronchitis bronchitis NN cord-329245-6tj2k1yn 290 7 virus virus NN cord-329245-6tj2k1yn 290 8 E e NN cord-329245-6tj2k1yn 290 9 protein protein NN cord-329245-6tj2k1yn 290 10 directs direct VBZ cord-329245-6tj2k1yn 290 11 Golgi Golgi NNP cord-329245-6tj2k1yn 290 12 targeting target VBG cord-329245-6tj2k1yn 290 13 Coronavirus Coronavirus NNP cord-329245-6tj2k1yn 290 14 particle particle NN cord-329245-6tj2k1yn 290 15 assembly assembly NN cord-329245-6tj2k1yn 290 16 : : : cord-329245-6tj2k1yn 290 17 primary primary JJ cord-329245-6tj2k1yn 290 18 structure structure NN cord-329245-6tj2k1yn 290 19 requirements requirement NNS cord-329245-6tj2k1yn 290 20 of of IN cord-329245-6tj2k1yn 290 21 the the DT cord-329245-6tj2k1yn 290 22 membrane membrane NN cord-329245-6tj2k1yn 290 23 protein protein NN cord-329245-6tj2k1yn 290 24 Assembly Assembly NNP cord-329245-6tj2k1yn 290 25 of of IN cord-329245-6tj2k1yn 290 26 the the DT cord-329245-6tj2k1yn 290 27 coronavirus coronavirus NN cord-329245-6tj2k1yn 290 28 envelope envelope NN cord-329245-6tj2k1yn 290 29 : : : cord-329245-6tj2k1yn 290 30 homotypic homotypic JJ cord-329245-6tj2k1yn 290 31 interactions interaction NNS cord-329245-6tj2k1yn 290 32 between between IN cord-329245-6tj2k1yn 290 33 the the DT cord-329245-6tj2k1yn 290 34 M M NNP cord-329245-6tj2k1yn 290 35 proteins protein NNS cord-329245-6tj2k1yn 290 36 Assembly Assembly NNP cord-329245-6tj2k1yn 290 37 of of IN cord-329245-6tj2k1yn 290 38 Enveloped Enveloped NNP cord-329245-6tj2k1yn 290 39 RNA RNA NNP cord-329245-6tj2k1yn 290 40 Viruses Viruses NNPS cord-329245-6tj2k1yn 290 41 Cytoplasmic Cytoplasmic NNP cord-329245-6tj2k1yn 290 42 expression expression NN cord-329245-6tj2k1yn 290 43 system system NN cord-329245-6tj2k1yn 290 44 based base VBN cord-329245-6tj2k1yn 290 45 on on IN cord-329245-6tj2k1yn 290 46 constitutive constitutive JJ cord-329245-6tj2k1yn 290 47 synthesis synthesis NN cord-329245-6tj2k1yn 290 48 of of IN cord-329245-6tj2k1yn 290 49 bacteriophage bacteriophage NN cord-329245-6tj2k1yn 290 50 T7 T7 NNP cord-329245-6tj2k1yn 290 51 RNA RNA NNP cord-329245-6tj2k1yn 290 52 polymerase polymerase NN cord-329245-6tj2k1yn 290 53 in in IN cord-329245-6tj2k1yn 290 54 mammalian mammalian JJ cord-329245-6tj2k1yn 290 55 cells cell NNS cord-329245-6tj2k1yn 290 56 Analysis Analysis NNP cord-329245-6tj2k1yn 290 57 of of IN cord-329245-6tj2k1yn 290 58 constructed construct VBN cord-329245-6tj2k1yn 290 59 E e NN cord-329245-6tj2k1yn 290 60 gene gene NN cord-329245-6tj2k1yn 290 61 mutants mutant NNS cord-329245-6tj2k1yn 290 62 of of IN cord-329245-6tj2k1yn 290 63 mouse mouse NNP cord-329245-6tj2k1yn 290 64 hepatitis hepatitis NNP cord-329245-6tj2k1yn 290 65 virus virus NNP cord-329245-6tj2k1yn 290 66 confirms confirm VBZ cord-329245-6tj2k1yn 290 67 a a DT cord-329245-6tj2k1yn 290 68 pivotal pivotal JJ cord-329245-6tj2k1yn 290 69 role role NN cord-329245-6tj2k1yn 290 70 for for IN cord-329245-6tj2k1yn 290 71 E e NN cord-329245-6tj2k1yn 290 72 protein protein NN cord-329245-6tj2k1yn 290 73 in in IN cord-329245-6tj2k1yn 290 74 coronavirus coronavirus NN cord-329245-6tj2k1yn 290 75 assembly assembly NN cord-329245-6tj2k1yn 290 76 Eukaryotic eukaryotic JJ cord-329245-6tj2k1yn 290 77 transient transient JJ cord-329245-6tj2k1yn 290 78 expression expression NN cord-329245-6tj2k1yn 290 79 system system NN cord-329245-6tj2k1yn 290 80 based base VBN cord-329245-6tj2k1yn 290 81 on on IN cord-329245-6tj2k1yn 290 82 recombinant recombinant JJ cord-329245-6tj2k1yn 290 83 vaccinia vaccinia NN cord-329245-6tj2k1yn 290 84 virus virus NN cord-329245-6tj2k1yn 290 85 that that WDT cord-329245-6tj2k1yn 290 86 synthesizes synthesize VBZ cord-329245-6tj2k1yn 290 87 bacteriophage bacteriophage NN cord-329245-6tj2k1yn 290 88 T7 T7 NNP cord-329245-6tj2k1yn 290 89 RNA RNA NNP cord-329245-6tj2k1yn 290 90 polymerase polymerase NN cord-329245-6tj2k1yn 290 91 Virus virus NN cord-329245-6tj2k1yn 290 92 maturation maturation NN cord-329245-6tj2k1yn 290 93 by by IN cord-329245-6tj2k1yn 290 94 budding bud VBG cord-329245-6tj2k1yn 290 95 . . . cord-329245-6tj2k1yn 291 1 Microbiol Microbiol NNP cord-329245-6tj2k1yn 291 2 Assembly Assembly NNP cord-329245-6tj2k1yn 291 3 of of IN cord-329245-6tj2k1yn 291 4 spikes spike NNS cord-329245-6tj2k1yn 291 5 into into IN cord-329245-6tj2k1yn 291 6 coronavirus coronavirus NN cord-329245-6tj2k1yn 291 7 particles particle NNS cord-329245-6tj2k1yn 291 8 is be VBZ cord-329245-6tj2k1yn 291 9 mediated mediate VBN cord-329245-6tj2k1yn 291 10 by by IN cord-329245-6tj2k1yn 291 11 the the DT cord-329245-6tj2k1yn 291 12 carboxy carboxy JJ cord-329245-6tj2k1yn 291 13 - - HYPH cord-329245-6tj2k1yn 291 14 terminal terminal NN cord-329245-6tj2k1yn 291 15 domain domain NN cord-329245-6tj2k1yn 291 16 of of IN cord-329245-6tj2k1yn 291 17 the the DT cord-329245-6tj2k1yn 291 18 spike spike NN cord-329245-6tj2k1yn 291 19 protein protein NN cord-329245-6tj2k1yn 291 20 Cell cell NN cord-329245-6tj2k1yn 291 21 biology biology NN cord-329245-6tj2k1yn 291 22 of of IN cord-329245-6tj2k1yn 291 23 viruses virus NNS cord-329245-6tj2k1yn 291 24 that that WDT cord-329245-6tj2k1yn 291 25 assemble assemble VBP cord-329245-6tj2k1yn 291 26 along along IN cord-329245-6tj2k1yn 291 27 the the DT cord-329245-6tj2k1yn 291 28 biosynthetic biosynthetic JJ cord-329245-6tj2k1yn 291 29 pathway pathway NN cord-329245-6tj2k1yn 291 30 Morphology morphology NN cord-329245-6tj2k1yn 291 31 and and CC cord-329245-6tj2k1yn 291 32 morphogenesis morphogenesis NN cord-329245-6tj2k1yn 291 33 of of IN cord-329245-6tj2k1yn 291 34 viruses virus NNS cord-329245-6tj2k1yn 291 35 of of IN cord-329245-6tj2k1yn 291 36 hemorrhagic hemorrhagic JJ cord-329245-6tj2k1yn 291 37 fever fever NN cord-329245-6tj2k1yn 291 38 with with IN cord-329245-6tj2k1yn 291 39 renal renal JJ cord-329245-6tj2k1yn 291 40 syndrome syndrome NN cord-329245-6tj2k1yn 291 41 ( ( -LRB- cord-329245-6tj2k1yn 291 42 HFRS HFRS NNP cord-329245-6tj2k1yn 291 43 ) ) -RRB- cord-329245-6tj2k1yn 291 44 . . . cord-329245-6tj2k1yn 292 1 I. I. NNP cord-329245-6tj2k1yn 293 1 Some some DT cord-329245-6tj2k1yn 293 2 peculiar peculiar JJ cord-329245-6tj2k1yn 293 3 aspects aspect NNS cord-329245-6tj2k1yn 293 4 of of IN cord-329245-6tj2k1yn 293 5 the the DT cord-329245-6tj2k1yn 293 6 morphogenesis morphogenesis NN cord-329245-6tj2k1yn 293 7 of of IN cord-329245-6tj2k1yn 293 8 various various JJ cord-329245-6tj2k1yn 293 9 strains strain NNS cord-329245-6tj2k1yn 293 10 of of IN cord-329245-6tj2k1yn 293 11 HFRS HFRS NNP cord-329245-6tj2k1yn 293 12 virus virus NN cord-329245-6tj2k1yn 293 13 Coronavirus Coronavirus NNP cord-329245-6tj2k1yn 293 14 M M NNP cord-329245-6tj2k1yn 293 15 proteins protein NNS cord-329245-6tj2k1yn 293 16 accumulate accumulate VBP cord-329245-6tj2k1yn 293 17 in in IN cord-329245-6tj2k1yn 293 18 the the DT cord-329245-6tj2k1yn 293 19 Golgi Golgi NNP cord-329245-6tj2k1yn 293 20 complex complex NN cord-329245-6tj2k1yn 293 21 beyond beyond IN cord-329245-6tj2k1yn 293 22 the the DT cord-329245-6tj2k1yn 293 23 site site NN cord-329245-6tj2k1yn 293 24 of of IN cord-329245-6tj2k1yn 293 25 virion virion NN cord-329245-6tj2k1yn 293 26 budding bud VBG cord-329245-6tj2k1yn 293 27 The the DT cord-329245-6tj2k1yn 293 28 missing missing JJ cord-329245-6tj2k1yn 293 29 link link NN cord-329245-6tj2k1yn 293 30 in in IN cord-329245-6tj2k1yn 293 31 coronavirus coronavirus NN cord-329245-6tj2k1yn 293 32 assembly assembly NN cord-329245-6tj2k1yn 293 33 . . . cord-329245-6tj2k1yn 294 1 Retention retention NN cord-329245-6tj2k1yn 294 2 of of IN cord-329245-6tj2k1yn 294 3 the the DT cord-329245-6tj2k1yn 294 4 avian avian JJ cord-329245-6tj2k1yn 294 5 coronavirus coronavirus NN cord-329245-6tj2k1yn 294 6 infectious infectious JJ cord-329245-6tj2k1yn 294 7 bronchitis bronchitis NN cord-329245-6tj2k1yn 294 8 virus virus NN cord-329245-6tj2k1yn 294 9 envelope envelope NN cord-329245-6tj2k1yn 294 10 protein protein NN cord-329245-6tj2k1yn 294 11 in in IN cord-329245-6tj2k1yn 294 12 the the DT cord-329245-6tj2k1yn 294 13 pre pre JJ cord-329245-6tj2k1yn 294 14 - - JJ cord-329245-6tj2k1yn 294 15 Golgi golgi JJ cord-329245-6tj2k1yn 294 16 compartments compartment NNS cord-329245-6tj2k1yn 294 17 and and CC cord-329245-6tj2k1yn 294 18 physical physical JJ cord-329245-6tj2k1yn 294 19 interaction interaction NN cord-329245-6tj2k1yn 294 20 between between IN cord-329245-6tj2k1yn 294 21 the the DT cord-329245-6tj2k1yn 294 22 envelope envelope NN cord-329245-6tj2k1yn 294 23 and and CC cord-329245-6tj2k1yn 294 24 membrane membrane NN cord-329245-6tj2k1yn 294 25 proteins protein NNS cord-329245-6tj2k1yn 294 26 Association Association NNP cord-329245-6tj2k1yn 294 27 of of IN cord-329245-6tj2k1yn 294 28 the the DT cord-329245-6tj2k1yn 294 29 infectious infectious JJ cord-329245-6tj2k1yn 294 30 bronchitis bronchitis NN cord-329245-6tj2k1yn 294 31 virus virus NN cord-329245-6tj2k1yn 294 32 3c 3c NNP cord-329245-6tj2k1yn 294 33 protein protein NN cord-329245-6tj2k1yn 294 34 with with IN cord-329245-6tj2k1yn 294 35 the the DT cord-329245-6tj2k1yn 294 36 virion virion NN cord-329245-6tj2k1yn 294 37 envelope envelope NN cord-329245-6tj2k1yn 294 38 Retention Retention NNP cord-329245-6tj2k1yn 294 39 of of IN cord-329245-6tj2k1yn 294 40 a a DT cord-329245-6tj2k1yn 294 41 cis cis NNP cord-329245-6tj2k1yn 294 42 Golgi Golgi NNP cord-329245-6tj2k1yn 294 43 protein protein NN cord-329245-6tj2k1yn 294 44 requires require VBZ cord-329245-6tj2k1yn 294 45 polar polar JJ cord-329245-6tj2k1yn 294 46 residues residue NNS cord-329245-6tj2k1yn 294 47 on on IN cord-329245-6tj2k1yn 294 48 one one CD cord-329245-6tj2k1yn 294 49 face face NN cord-329245-6tj2k1yn 294 50 of of IN cord-329245-6tj2k1yn 294 51 a a DT cord-329245-6tj2k1yn 294 52 predicted predict VBN cord-329245-6tj2k1yn 294 53 alpha alpha NN cord-329245-6tj2k1yn 294 54 - - NN cord-329245-6tj2k1yn 294 55 helix helix NN cord-329245-6tj2k1yn 294 56 in in IN cord-329245-6tj2k1yn 294 57 the the DT cord-329245-6tj2k1yn 294 58 transmembrane transmembrane NN cord-329245-6tj2k1yn 294 59 domain domain NN cord-329245-6tj2k1yn 295 1 The the DT cord-329245-6tj2k1yn 295 2 E1 e1 NN cord-329245-6tj2k1yn 295 3 glycoprotein glycoprotein NN cord-329245-6tj2k1yn 295 4 of of IN cord-329245-6tj2k1yn 295 5 an an DT cord-329245-6tj2k1yn 295 6 avian avian JJ cord-329245-6tj2k1yn 295 7 coronavirus coronavirus NN cord-329245-6tj2k1yn 295 8 is be VBZ cord-329245-6tj2k1yn 295 9 targeted target VBN cord-329245-6tj2k1yn 295 10 to to IN cord-329245-6tj2k1yn 295 11 the the DT cord-329245-6tj2k1yn 295 12 cis cis NNP cord-329245-6tj2k1yn 295 13 Golgi Golgi NNP cord-329245-6tj2k1yn 295 14 complex complex NN cord-329245-6tj2k1yn 295 15 A a DT cord-329245-6tj2k1yn 295 16 specific specific JJ cord-329245-6tj2k1yn 295 17 transmembrane transmembrane NN cord-329245-6tj2k1yn 295 18 domain domain NN cord-329245-6tj2k1yn 295 19 of of IN cord-329245-6tj2k1yn 295 20 a a DT cord-329245-6tj2k1yn 295 21 coronavirus coronavirus NN cord-329245-6tj2k1yn 295 22 E1 e1 NN cord-329245-6tj2k1yn 295 23 glycoprotein glycoprotein NN cord-329245-6tj2k1yn 295 24 is be VBZ cord-329245-6tj2k1yn 295 25 required require VBN cord-329245-6tj2k1yn 295 26 for for IN cord-329245-6tj2k1yn 295 27 its -PRON- PRP$ cord-329245-6tj2k1yn 295 28 retention retention NN cord-329245-6tj2k1yn 295 29 in in IN cord-329245-6tj2k1yn 295 30 the the DT cord-329245-6tj2k1yn 295 31 Golgi Golgi NNP cord-329245-6tj2k1yn 295 32 region region NN cord-329245-6tj2k1yn 295 33 Assembly assembly NN cord-329245-6tj2k1yn 295 34 and and CC cord-329245-6tj2k1yn 295 35 maturation maturation NN cord-329245-6tj2k1yn 295 36 of of IN cord-329245-6tj2k1yn 295 37 the the DT cord-329245-6tj2k1yn 295 38 flavivirus flavivirus NNP cord-329245-6tj2k1yn 295 39 kunjin kunjin NNP cord-329245-6tj2k1yn 295 40 virus virus NN cord-329245-6tj2k1yn 295 41 appear appear VBP cord-329245-6tj2k1yn 295 42 to to TO cord-329245-6tj2k1yn 295 43 occur occur VB cord-329245-6tj2k1yn 295 44 in in IN cord-329245-6tj2k1yn 295 45 the the DT cord-329245-6tj2k1yn 295 46 rough rough JJ cord-329245-6tj2k1yn 295 47 endoplasmic endoplasmic NN cord-329245-6tj2k1yn 295 48 reticulum reticulum NN cord-329245-6tj2k1yn 295 49 and and CC cord-329245-6tj2k1yn 295 50 along along IN cord-329245-6tj2k1yn 295 51 the the DT cord-329245-6tj2k1yn 295 52 secretory secretory JJ cord-329245-6tj2k1yn 295 53 pathway pathway NN cord-329245-6tj2k1yn 295 54 , , , cord-329245-6tj2k1yn 295 55 respectively respectively RB cord-329245-6tj2k1yn 295 56 Release Release NNP cord-329245-6tj2k1yn 295 57 of of IN cord-329245-6tj2k1yn 295 58 coronavirus coronavirus NN cord-329245-6tj2k1yn 295 59 E e NN cord-329245-6tj2k1yn 295 60 protein protein NN cord-329245-6tj2k1yn 295 61 in in IN cord-329245-6tj2k1yn 295 62 membrane membrane NN cord-329245-6tj2k1yn 295 63 vesicles vesicle NNS cord-329245-6tj2k1yn 295 64 from from IN cord-329245-6tj2k1yn 295 65 virus virus NN cord-329245-6tj2k1yn 295 66 - - HYPH cord-329245-6tj2k1yn 295 67 infected infect VBN cord-329245-6tj2k1yn 295 68 cells cell NNS cord-329245-6tj2k1yn 295 69 and and CC cord-329245-6tj2k1yn 295 70 E e NN cord-329245-6tj2k1yn 295 71 protein protein NN cord-329245-6tj2k1yn 295 72 - - HYPH cord-329245-6tj2k1yn 295 73 expressing express VBG cord-329245-6tj2k1yn 295 74 cells cell NNS cord-329245-6tj2k1yn 295 75 Protein Protein NNP cord-329245-6tj2k1yn 295 76 localization localization NN cord-329245-6tj2k1yn 295 77 and and CC cord-329245-6tj2k1yn 295 78 virus virus NN cord-329245-6tj2k1yn 295 79 assembly assembly NN cord-329245-6tj2k1yn 295 80 at at IN cord-329245-6tj2k1yn 295 81 intracellular intracellular JJ cord-329245-6tj2k1yn 295 82 membranes membrane NNS cord-329245-6tj2k1yn 295 83 Matrix Matrix NNP cord-329245-6tj2k1yn 295 84 proteins protein NNS cord-329245-6tj2k1yn 295 85 can can MD cord-329245-6tj2k1yn 295 86 generate generate VB cord-329245-6tj2k1yn 295 87 the the DT cord-329245-6tj2k1yn 295 88 higher high JJR cord-329245-6tj2k1yn 295 89 order order NN cord-329245-6tj2k1yn 295 90 architecture architecture NN cord-329245-6tj2k1yn 295 91 of of IN cord-329245-6tj2k1yn 295 92 the the DT cord-329245-6tj2k1yn 295 93 Golgi Golgi NNP cord-329245-6tj2k1yn 295 94 apparatus apparatus NN cord-329245-6tj2k1yn 296 1 The the DT cord-329245-6tj2k1yn 296 2 role role NN cord-329245-6tj2k1yn 296 3 of of IN cord-329245-6tj2k1yn 296 4 the the DT cord-329245-6tj2k1yn 296 5 tethering tethering NN cord-329245-6tj2k1yn 296 6 proteins protein NNS cord-329245-6tj2k1yn 296 7 p115 p115 NNP cord-329245-6tj2k1yn 296 8 and and CC cord-329245-6tj2k1yn 296 9 GM130 GM130 NNP cord-329245-6tj2k1yn 296 10 in in IN cord-329245-6tj2k1yn 296 11 transport transport NN cord-329245-6tj2k1yn 296 12 through through IN cord-329245-6tj2k1yn 296 13 the the DT cord-329245-6tj2k1yn 296 14 Golgi Golgi NNP cord-329245-6tj2k1yn 296 15 apparatus apparatus NN cord-329245-6tj2k1yn 296 16 in in IN cord-329245-6tj2k1yn 296 17 vivo vivo NNP cord-329245-6tj2k1yn 296 18 Structural structural JJ cord-329245-6tj2k1yn 296 19 analysis analysis NN cord-329245-6tj2k1yn 296 20 of of IN cord-329245-6tj2k1yn 296 21 virion virion NN cord-329245-6tj2k1yn 296 22 proteins protein NNS cord-329245-6tj2k1yn 296 23 of of IN cord-329245-6tj2k1yn 296 24 the the DT cord-329245-6tj2k1yn 296 25 avian avian JJ cord-329245-6tj2k1yn 296 26 coronavirus coronavirus NN cord-329245-6tj2k1yn 296 27 infectious infectious JJ cord-329245-6tj2k1yn 296 28 bronchitis bronchitis NN cord-329245-6tj2k1yn 296 29 virus virus NN cord-329245-6tj2k1yn 296 30 Isolation isolation NN cord-329245-6tj2k1yn 296 31 of of IN cord-329245-6tj2k1yn 296 32 coronavirus coronavirus NN cord-329245-6tj2k1yn 296 33 envelope envelope NN cord-329245-6tj2k1yn 296 34 glycoproteins glycoprotein NNS cord-329245-6tj2k1yn 296 35 and and CC cord-329245-6tj2k1yn 296 36 interaction interaction NN cord-329245-6tj2k1yn 296 37 with with IN cord-329245-6tj2k1yn 296 38 the the DT cord-329245-6tj2k1yn 296 39 viral viral JJ cord-329245-6tj2k1yn 296 40 nucleocapsid nucleocapsid NN cord-329245-6tj2k1yn 296 41 A a DT cord-329245-6tj2k1yn 296 42 Golgi Golgi NNP cord-329245-6tj2k1yn 296 43 retention retention NN cord-329245-6tj2k1yn 296 44 signal signal NN cord-329245-6tj2k1yn 296 45 in in IN cord-329245-6tj2k1yn 296 46 a a DT cord-329245-6tj2k1yn 296 47 membrane membrane NN cord-329245-6tj2k1yn 296 48 - - HYPH cord-329245-6tj2k1yn 296 49 spanning span VBG cord-329245-6tj2k1yn 296 50 domain domain NN cord-329245-6tj2k1yn 296 51 of of IN cord-329245-6tj2k1yn 296 52 coronavirus coronavirus NN cord-329245-6tj2k1yn 296 53 E1 e1 NN cord-329245-6tj2k1yn 296 54 protein protein NN cord-329245-6tj2k1yn 296 55 Nucleocapsid Nucleocapsid NNP cord-329245-6tj2k1yn 296 56 - - HYPH cord-329245-6tj2k1yn 296 57 independent independent JJ cord-329245-6tj2k1yn 296 58 assembly assembly NN cord-329245-6tj2k1yn 296 59 of of IN cord-329245-6tj2k1yn 296 60 coronavirus coronavirus NN cord-329245-6tj2k1yn 296 61 - - HYPH cord-329245-6tj2k1yn 296 62 like like JJ cord-329245-6tj2k1yn 296 63 particles particle NNS cord-329245-6tj2k1yn 296 64 by by IN cord-329245-6tj2k1yn 296 65 co co NN cord-329245-6tj2k1yn 296 66 - - NN cord-329245-6tj2k1yn 296 67 expression expression NN cord-329245-6tj2k1yn 296 68 of of IN cord-329245-6tj2k1yn 296 69 viral viral JJ cord-329245-6tj2k1yn 296 70 envelope envelope NN cord-329245-6tj2k1yn 296 71 protein protein NN cord-329245-6tj2k1yn 296 72 genes gene NNS cord-329245-6tj2k1yn 296 73 Use Use NNP cord-329245-6tj2k1yn 296 74 of of IN cord-329245-6tj2k1yn 296 75 recombinant recombinant JJ cord-329245-6tj2k1yn 296 76 vaccinia vaccinia NN cord-329245-6tj2k1yn 296 77 virus virus NN cord-329245-6tj2k1yn 296 78 vectors vector NNS cord-329245-6tj2k1yn 296 79 for for IN cord-329245-6tj2k1yn 296 80 cell cell NN cord-329245-6tj2k1yn 296 81 biology biology NN cord-329245-6tj2k1yn 296 82 Oligomerization Oligomerization NNP cord-329245-6tj2k1yn 296 83 of of IN cord-329245-6tj2k1yn 296 84 a a DT cord-329245-6tj2k1yn 296 85 membrane membrane NN cord-329245-6tj2k1yn 296 86 protein protein NN cord-329245-6tj2k1yn 296 87 correlates correlate VBZ cord-329245-6tj2k1yn 296 88 with with IN cord-329245-6tj2k1yn 296 89 its -PRON- PRP$ cord-329245-6tj2k1yn 296 90 retention retention NN cord-329245-6tj2k1yn 296 91 in in IN cord-329245-6tj2k1yn 296 92 the the DT cord-329245-6tj2k1yn 296 93 Golgi Golgi NNP cord-329245-6tj2k1yn 296 94 complex complex NN cord-329245-6tj2k1yn 297 1 This this DT cord-329245-6tj2k1yn 297 2 work work NN cord-329245-6tj2k1yn 297 3 was be VBD cord-329245-6tj2k1yn 297 4 supported support VBN cord-329245-6tj2k1yn 297 5 by by IN cord-329245-6tj2k1yn 297 6 National National NNP cord-329245-6tj2k1yn 297 7 Institutes Institutes NNPS cord-329245-6tj2k1yn 297 8 of of IN cord-329245-6tj2k1yn 297 9 Health Health NNP cord-329245-6tj2k1yn 297 10 Grants Grants NNPS cord-329245-6tj2k1yn 297 11 GM42522 gm42522 NN cord-329245-6tj2k1yn 297 12 and and CC cord-329245-6tj2k1yn 297 13 GM64647 GM64647 NNP cord-329245-6tj2k1yn 297 14 . . . cord-329245-6tj2k1yn 298 1 We -PRON- PRP cord-329245-6tj2k1yn 298 2 thank thank VBP cord-329245-6tj2k1yn 298 3 the the DT cord-329245-6tj2k1yn 298 4 members member NNS cord-329245-6tj2k1yn 298 5 of of IN cord-329245-6tj2k1yn 298 6 the the DT cord-329245-6tj2k1yn 298 7 Machamer Machamer NNP cord-329245-6tj2k1yn 298 8 lab lab NN cord-329245-6tj2k1yn 298 9 for for IN cord-329245-6tj2k1yn 298 10 critical critical JJ cord-329245-6tj2k1yn 298 11 reading reading NN cord-329245-6tj2k1yn 298 12 of of IN cord-329245-6tj2k1yn 298 13 the the DT cord-329245-6tj2k1yn 298 14 manuscript manuscript NN cord-329245-6tj2k1yn 298 15 . . .