id	sid	tid	token	lemma	pos
cord-345088-krb1eidw	1	1	key	key	JJ
cord-345088-krb1eidw	1	2	:	:	:
cord-345088-krb1eidw	1	3	cord-345088-krb1eidw	cord-345088-krb1eidw	JJ
cord-345088-krb1eidw	1	4	authors	author	NNS
cord-345088-krb1eidw	1	5	:	:	:
cord-345088-krb1eidw	1	6	Shen	Shen	NNP
cord-345088-krb1eidw	1	7	,	,	,
cord-345088-krb1eidw	1	8	S	S	NNP
cord-345088-krb1eidw	1	9	;	;	:
cord-345088-krb1eidw	1	10	Law	Law	NNP
cord-345088-krb1eidw	1	11	,	,	,
cord-345088-krb1eidw	1	12	Y.C	Y.C	NNP
cord-345088-krb1eidw	1	13	;	;	:
cord-345088-krb1eidw	1	14	Liu	Liu	NNP
cord-345088-krb1eidw	1	15	,	,	,
cord-345088-krb1eidw	2	1	D.X	D.X	NNP
cord-345088-krb1eidw	2	2	title	title	NN
cord-345088-krb1eidw	2	3	:	:	:
cord-345088-krb1eidw	2	4	A	a	DT
cord-345088-krb1eidw	2	5	single	single	JJ
cord-345088-krb1eidw	2	6	amino	amino	NN
cord-345088-krb1eidw	2	7	acid	acid	NN
cord-345088-krb1eidw	2	8	mutation	mutation	NN
cord-345088-krb1eidw	2	9	in	in	IN
cord-345088-krb1eidw	2	10	the	the	DT
cord-345088-krb1eidw	2	11	spike	spike	NN
cord-345088-krb1eidw	2	12	protein	protein	NN
cord-345088-krb1eidw	2	13	of	of	IN
cord-345088-krb1eidw	2	14	coronavirus	coronavirus	NN
cord-345088-krb1eidw	2	15	infectious	infectious	JJ
cord-345088-krb1eidw	2	16	bronchitis	bronchitis	NN
cord-345088-krb1eidw	2	17	virus	virus	NN
cord-345088-krb1eidw	2	18	hampers	hamper	VBZ
cord-345088-krb1eidw	2	19	its	-PRON-	PRP$
cord-345088-krb1eidw	2	20	maturation	maturation	NN
cord-345088-krb1eidw	2	21	and	and	CC
cord-345088-krb1eidw	2	22	incorporation	incorporation	NN
cord-345088-krb1eidw	2	23	into	into	IN
cord-345088-krb1eidw	2	24	virions	virion	NNS
cord-345088-krb1eidw	2	25	at	at	IN
cord-345088-krb1eidw	2	26	the	the	DT
cord-345088-krb1eidw	2	27	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	2	28	temperature	temperature	NN
cord-345088-krb1eidw	2	29	date	date	NN
cord-345088-krb1eidw	3	1	:	:	:
cord-345088-krb1eidw	3	2	2004	2004	CD
cord-345088-krb1eidw	3	3	-	-	SYM
cord-345088-krb1eidw	3	4	09	09	CD
cord-345088-krb1eidw	3	5	-	-	HYPH
cord-345088-krb1eidw	3	6	01	01	CD
cord-345088-krb1eidw	3	7	journal	journal	NN
cord-345088-krb1eidw	3	8	:	:	:
cord-345088-krb1eidw	4	1	Virology	virology	NN
cord-345088-krb1eidw	4	2	DOI	DOI	NNP
cord-345088-krb1eidw	4	3	:	:	:
cord-345088-krb1eidw	4	4	10.1016	10.1016	CD
cord-345088-krb1eidw	4	5	/	/	SYM
cord-345088-krb1eidw	4	6	j.virol.2004.06.016	j.virol.2004.06.016	UH
cord-345088-krb1eidw	4	7	sha	sha	NN
cord-345088-krb1eidw	4	8	:	:	:
cord-345088-krb1eidw	4	9	40b31b93eef7fc336903f06b272c89a161f01c21	40b31b93eef7fc336903f06b272c89a161f01c21	CD
cord-345088-krb1eidw	4	10	doc_id	doc_id	CD
cord-345088-krb1eidw	4	11	:	:	:
cord-345088-krb1eidw	4	12	345088	345088	CD
cord-345088-krb1eidw	4	13	cord_uid	cord_uid	NNS
cord-345088-krb1eidw	4	14	:	:	:
cord-345088-krb1eidw	5	1	krb1eidw	krb1eidw	NNP
cord-345088-krb1eidw	6	1	The	the	DT
cord-345088-krb1eidw	6	2	spike	spike	NN
cord-345088-krb1eidw	6	3	(	(	-LRB-
cord-345088-krb1eidw	6	4	S	s	NN
cord-345088-krb1eidw	6	5	)	)	-RRB-
cord-345088-krb1eidw	6	6	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	6	7	of	of	IN
cord-345088-krb1eidw	6	8	coronavirus	coronavirus	NN
cord-345088-krb1eidw	6	9	is	be	VBZ
cord-345088-krb1eidw	6	10	responsible	responsible	JJ
cord-345088-krb1eidw	6	11	for	for	IN
cord-345088-krb1eidw	6	12	receptor	receptor	NN
cord-345088-krb1eidw	6	13	binding	binding	NN
cord-345088-krb1eidw	6	14	and	and	CC
cord-345088-krb1eidw	6	15	membrane	membrane	NN
cord-345088-krb1eidw	6	16	fusion	fusion	NN
cord-345088-krb1eidw	6	17	.	.	.
cord-345088-krb1eidw	7	1	A	a	DT
cord-345088-krb1eidw	7	2	number	number	NN
cord-345088-krb1eidw	7	3	of	of	IN
cord-345088-krb1eidw	7	4	variants	variant	NNS
cord-345088-krb1eidw	7	5	with	with	IN
cord-345088-krb1eidw	7	6	deletions	deletion	NNS
cord-345088-krb1eidw	7	7	and	and	CC
cord-345088-krb1eidw	7	8	mutations	mutation	NNS
cord-345088-krb1eidw	7	9	in	in	IN
cord-345088-krb1eidw	7	10	the	the	DT
cord-345088-krb1eidw	7	11	S	S	NNP
cord-345088-krb1eidw	7	12	protein	protein	NN
cord-345088-krb1eidw	7	13	have	have	VBP
cord-345088-krb1eidw	7	14	been	be	VBN
cord-345088-krb1eidw	7	15	isolated	isolate	VBN
cord-345088-krb1eidw	7	16	from	from	IN
cord-345088-krb1eidw	7	17	naturally	naturally	RB
cord-345088-krb1eidw	7	18	and	and	CC
cord-345088-krb1eidw	7	19	persistently	persistently	RB
cord-345088-krb1eidw	7	20	infected	infected	JJ
cord-345088-krb1eidw	7	21	animals	animal	NNS
cord-345088-krb1eidw	7	22	and	and	CC
cord-345088-krb1eidw	7	23	tissue	tissue	NN
cord-345088-krb1eidw	7	24	cultures	culture	NNS
cord-345088-krb1eidw	7	25	.	.	.
cord-345088-krb1eidw	8	1	Here	here	RB
cord-345088-krb1eidw	8	2	,	,	,
cord-345088-krb1eidw	8	3	we	-PRON-	PRP
cord-345088-krb1eidw	8	4	report	report	VBP
cord-345088-krb1eidw	8	5	the	the	DT
cord-345088-krb1eidw	8	6	emergence	emergence	NN
cord-345088-krb1eidw	8	7	and	and	CC
cord-345088-krb1eidw	8	8	isolation	isolation	NN
cord-345088-krb1eidw	8	9	of	of	IN
cord-345088-krb1eidw	8	10	two	two	CD
cord-345088-krb1eidw	8	11	temperature	temperature	NN
cord-345088-krb1eidw	8	12	sensitive	sensitive	JJ
cord-345088-krb1eidw	8	13	(	(	-LRB-
cord-345088-krb1eidw	8	14	ts	ts	NNP
cord-345088-krb1eidw	8	15	)	)	-RRB-
cord-345088-krb1eidw	8	16	mutants	mutant	NNS
cord-345088-krb1eidw	8	17	and	and	CC
cord-345088-krb1eidw	8	18	a	a	DT
cord-345088-krb1eidw	8	19	revertant	revertant	NN
cord-345088-krb1eidw	8	20	in	in	IN
cord-345088-krb1eidw	8	21	the	the	DT
cord-345088-krb1eidw	8	22	process	process	NN
cord-345088-krb1eidw	8	23	of	of	IN
cord-345088-krb1eidw	8	24	cold	cold	JJ
cord-345088-krb1eidw	8	25	-	-	HYPH
cord-345088-krb1eidw	8	26	adaptation	adaptation	NN
cord-345088-krb1eidw	8	27	of	of	IN
cord-345088-krb1eidw	8	28	coronavirus	coronavirus	NN
cord-345088-krb1eidw	8	29	infectious	infectious	JJ
cord-345088-krb1eidw	8	30	bronchitis	bronchitis	NN
cord-345088-krb1eidw	8	31	virus	virus	NN
cord-345088-krb1eidw	8	32	(	(	-LRB-
cord-345088-krb1eidw	8	33	IBV	IBV	NNP
cord-345088-krb1eidw	8	34	)	)	-RRB-
cord-345088-krb1eidw	8	35	to	to	IN
cord-345088-krb1eidw	8	36	a	a	DT
cord-345088-krb1eidw	8	37	monkey	monkey	NN
cord-345088-krb1eidw	8	38	kidney	kidney	NN
cord-345088-krb1eidw	8	39	cell	cell	NN
cord-345088-krb1eidw	8	40	line	line	NN
cord-345088-krb1eidw	8	41	.	.	.
cord-345088-krb1eidw	9	1	The	the	DT
cord-345088-krb1eidw	9	2	complete	complete	JJ
cord-345088-krb1eidw	9	3	sequences	sequence	NNS
cord-345088-krb1eidw	9	4	of	of	IN
cord-345088-krb1eidw	9	5	wild	wild	JJ
cord-345088-krb1eidw	9	6	type	type	NN
cord-345088-krb1eidw	9	7	(	(	-LRB-
cord-345088-krb1eidw	9	8	wt	wt	NN
cord-345088-krb1eidw	9	9	)	)	-RRB-
cord-345088-krb1eidw	9	10	virus	virus	NN
cord-345088-krb1eidw	9	11	,	,	,
cord-345088-krb1eidw	9	12	two	two	CD
cord-345088-krb1eidw	9	13	ts	ts	JJ
cord-345088-krb1eidw	9	14	mutants	mutant	NNS
cord-345088-krb1eidw	9	15	,	,	,
cord-345088-krb1eidw	9	16	and	and	CC
cord-345088-krb1eidw	9	17	the	the	DT
cord-345088-krb1eidw	9	18	revertant	revertant	NN
cord-345088-krb1eidw	9	19	were	be	VBD
cord-345088-krb1eidw	9	20	compared	compare	VBN
cord-345088-krb1eidw	9	21	and	and	CC
cord-345088-krb1eidw	9	22	variations	variation	NNS
cord-345088-krb1eidw	9	23	linked	link	VBN
cord-345088-krb1eidw	9	24	to	to	IN
cord-345088-krb1eidw	9	25	phenotypes	phenotype	NNS
cord-345088-krb1eidw	9	26	were	be	VBD
cord-345088-krb1eidw	9	27	mapped	map	VBN
cord-345088-krb1eidw	9	28	.	.	.
cord-345088-krb1eidw	10	1	A	a	DT
cord-345088-krb1eidw	10	2	single	single	JJ
cord-345088-krb1eidw	10	3	amino	amino	NN
cord-345088-krb1eidw	10	4	acid	acid	NN
cord-345088-krb1eidw	10	5	reversion	reversion	NN
cord-345088-krb1eidw	10	6	(	(	-LRB-
cord-345088-krb1eidw	10	7	L(294)-to	l(294)-to	NN
cord-345088-krb1eidw	10	8	-	-	HYPH
cord-345088-krb1eidw	10	9	Q	Q	NNP
cord-345088-krb1eidw	10	10	)	)	-RRB-
cord-345088-krb1eidw	10	11	in	in	IN
cord-345088-krb1eidw	10	12	the	the	DT
cord-345088-krb1eidw	10	13	S	S	NNP
cord-345088-krb1eidw	10	14	protein	protein	NN
cord-345088-krb1eidw	10	15	is	be	VBZ
cord-345088-krb1eidw	10	16	sufficient	sufficient	JJ
cord-345088-krb1eidw	10	17	to	to	TO
cord-345088-krb1eidw	10	18	abrogate	abrogate	VB
cord-345088-krb1eidw	10	19	the	the	DT
cord-345088-krb1eidw	10	20	ts	ts	NNP
cord-345088-krb1eidw	10	21	phenotype	phenotype	NN
cord-345088-krb1eidw	10	22	.	.	.
cord-345088-krb1eidw	11	1	Interestingly	interestingly	RB
cord-345088-krb1eidw	11	2	,	,	,
cord-345088-krb1eidw	11	3	unlike	unlike	IN
cord-345088-krb1eidw	11	4	wt	wt	NN
cord-345088-krb1eidw	11	5	virus	virus	NN
cord-345088-krb1eidw	11	6	,	,	,
cord-345088-krb1eidw	11	7	the	the	DT
cord-345088-krb1eidw	11	8	revertant	revertant	NN
cord-345088-krb1eidw	11	9	grows	grow	VBZ
cord-345088-krb1eidw	11	10	well	well	RB
cord-345088-krb1eidw	11	11	at	at	IN
cord-345088-krb1eidw	11	12	and	and	CC
cord-345088-krb1eidw	11	13	below	below	IN
cord-345088-krb1eidw	11	14	32	32	CD
cord-345088-krb1eidw	11	15	°	°	NN
cord-345088-krb1eidw	11	16	C	c	NN
cord-345088-krb1eidw	11	17	,	,	,
cord-345088-krb1eidw	11	18	the	the	DT
cord-345088-krb1eidw	11	19	permissive	permissive	JJ
cord-345088-krb1eidw	11	20	temperature	temperature	NN
cord-345088-krb1eidw	11	21	,	,	,
cord-345088-krb1eidw	11	22	as	as	IN
cord-345088-krb1eidw	11	23	it	-PRON-	PRP
cord-345088-krb1eidw	11	24	carries	carry	VBZ
cord-345088-krb1eidw	11	25	other	other	JJ
cord-345088-krb1eidw	11	26	mutations	mutation	NNS
cord-345088-krb1eidw	11	27	in	in	IN
cord-345088-krb1eidw	11	28	multiple	multiple	JJ
cord-345088-krb1eidw	11	29	genes	gene	NNS
cord-345088-krb1eidw	11	30	that	that	WDT
cord-345088-krb1eidw	11	31	might	may	MD
cord-345088-krb1eidw	11	32	be	be	VB
cord-345088-krb1eidw	11	33	associated	associate	VBN
cord-345088-krb1eidw	11	34	with	with	IN
cord-345088-krb1eidw	11	35	the	the	DT
cord-345088-krb1eidw	11	36	cold	cold	JJ
cord-345088-krb1eidw	11	37	-	-	HYPH
cord-345088-krb1eidw	11	38	adaptation	adaptation	NN
cord-345088-krb1eidw	11	39	phenotype	phenotype	NN
cord-345088-krb1eidw	11	40	.	.	.
cord-345088-krb1eidw	12	1	If	if	IN
cord-345088-krb1eidw	12	2	the	the	DT
cord-345088-krb1eidw	12	3	two	two	CD
cord-345088-krb1eidw	12	4	ts	t	NNS
cord-345088-krb1eidw	12	5	mutants	mutant	NNS
cord-345088-krb1eidw	12	6	were	be	VBD
cord-345088-krb1eidw	12	7	allowed	allow	VBN
cord-345088-krb1eidw	12	8	to	to	TO
cord-345088-krb1eidw	12	9	enter	enter	VB
cord-345088-krb1eidw	12	10	cells	cell	NNS
cord-345088-krb1eidw	12	11	at	at	IN
cord-345088-krb1eidw	12	12	32	32	CD
cord-345088-krb1eidw	12	13	°	°	NN
cord-345088-krb1eidw	12	14	C	c	NN
cord-345088-krb1eidw	12	15	,	,	,
cord-345088-krb1eidw	12	16	the	the	DT
cord-345088-krb1eidw	12	17	S	S	NNP
cord-345088-krb1eidw	12	18	protein	protein	NN
cord-345088-krb1eidw	12	19	was	be	VBD
cord-345088-krb1eidw	12	20	synthesized	synthesize	VBN
cord-345088-krb1eidw	12	21	,	,	,
cord-345088-krb1eidw	12	22	core	core	NN
cord-345088-krb1eidw	12	23	-	-	HYPH
cord-345088-krb1eidw	12	24	glycosylated	glycosylated	JJ
cord-345088-krb1eidw	12	25	and	and	CC
cord-345088-krb1eidw	12	26	at	at	IN
cord-345088-krb1eidw	12	27	least	least	JJS
cord-345088-krb1eidw	12	28	partially	partially	RB
cord-345088-krb1eidw	12	29	modified	modify	VBN
cord-345088-krb1eidw	12	30	at	at	IN
cord-345088-krb1eidw	12	31	40	40	CD
cord-345088-krb1eidw	12	32	°	°	NN
cord-345088-krb1eidw	12	33	C	c	NN
cord-345088-krb1eidw	12	34	.	.	.
cord-345088-krb1eidw	13	1	However	however	RB
cord-345088-krb1eidw	13	2	,	,	,
cord-345088-krb1eidw	13	3	compared	compare	VBN
cord-345088-krb1eidw	13	4	with	with	IN
cord-345088-krb1eidw	13	5	wt	wt	NN
cord-345088-krb1eidw	13	6	virus	virus	NN
cord-345088-krb1eidw	13	7	and	and	CC
cord-345088-krb1eidw	13	8	the	the	DT
cord-345088-krb1eidw	13	9	revertant	revertant	NN
cord-345088-krb1eidw	13	10	,	,	,
cord-345088-krb1eidw	13	11	no	no	DT
cord-345088-krb1eidw	13	12	infectious	infectious	JJ
cord-345088-krb1eidw	13	13	particles	particle	NNS
cord-345088-krb1eidw	13	14	of	of	IN
cord-345088-krb1eidw	13	15	these	these	DT
cord-345088-krb1eidw	13	16	ts	ts	JJ
cord-345088-krb1eidw	13	17	mutants	mutant	NNS
cord-345088-krb1eidw	13	18	were	be	VBD
cord-345088-krb1eidw	13	19	assembled	assemble	VBN
cord-345088-krb1eidw	13	20	and	and	CC
cord-345088-krb1eidw	13	21	released	release	VBN
cord-345088-krb1eidw	13	22	from	from	IN
cord-345088-krb1eidw	13	23	the	the	DT
cord-345088-krb1eidw	13	24	ts	ts	NNP
cord-345088-krb1eidw	13	25	mutant	mutant	NN
cord-345088-krb1eidw	13	26	-	-	HYPH
cord-345088-krb1eidw	13	27	infected	infect	VBN
cord-345088-krb1eidw	13	28	cells	cell	NNS
cord-345088-krb1eidw	13	29	at	at	IN
cord-345088-krb1eidw	13	30	40	40	CD
cord-345088-krb1eidw	13	31	°	°	NN
cord-345088-krb1eidw	13	32	C	c	NN
cord-345088-krb1eidw	13	33	.	.	.
cord-345088-krb1eidw	14	1	Evidence	evidence	NN
cord-345088-krb1eidw	14	2	presented	present	VBN
cord-345088-krb1eidw	14	3	demonstrated	demonstrate	VBD
cord-345088-krb1eidw	14	4	that	that	IN
cord-345088-krb1eidw	14	5	the	the	DT
cord-345088-krb1eidw	14	6	Q(294)-to	Q(294)-to	NNP
cord-345088-krb1eidw	14	7	-	-	HYPH
cord-345088-krb1eidw	14	8	L(294	L(294	NNP
cord-345088-krb1eidw	14	9	)	)	-RRB-
cord-345088-krb1eidw	14	10	mutation	mutation	NN
cord-345088-krb1eidw	14	11	,	,	,
cord-345088-krb1eidw	14	12	located	locate	VBN
cord-345088-krb1eidw	14	13	at	at	IN
cord-345088-krb1eidw	14	14	a	a	DT
cord-345088-krb1eidw	14	15	highly	highly	RB
cord-345088-krb1eidw	14	16	conserved	conserved	JJ
cord-345088-krb1eidw	14	17	domain	domain	NN
cord-345088-krb1eidw	14	18	of	of	IN
cord-345088-krb1eidw	14	19	the	the	DT
cord-345088-krb1eidw	14	20	S1	S1	NNP
cord-345088-krb1eidw	14	21	subunit	subunit	NN
cord-345088-krb1eidw	14	22	,	,	,
cord-345088-krb1eidw	14	23	might	may	MD
cord-345088-krb1eidw	14	24	hamper	hamper	VB
cord-345088-krb1eidw	14	25	processing	processing	NN
cord-345088-krb1eidw	14	26	of	of	IN
cord-345088-krb1eidw	14	27	the	the	DT
cord-345088-krb1eidw	14	28	S	S	NNP
cord-345088-krb1eidw	14	29	protein	protein	NN
cord-345088-krb1eidw	14	30	to	to	IN
cord-345088-krb1eidw	14	31	a	a	DT
cord-345088-krb1eidw	14	32	matured	mature	VBN
cord-345088-krb1eidw	14	33	180-kDa	180-kda	CD
cord-345088-krb1eidw	14	34	,	,	,
cord-345088-krb1eidw	14	35	endo	endo	NN
cord-345088-krb1eidw	14	36	-	-	HYPH
cord-345088-krb1eidw	14	37	glycosidase	glycosidase	NNP
cord-345088-krb1eidw	14	38	H	h	NN
cord-345088-krb1eidw	14	39	-	-	HYPH
cord-345088-krb1eidw	14	40	resistant	resistant	JJ
cord-345088-krb1eidw	14	41	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	14	42	and	and	CC
cord-345088-krb1eidw	14	43	the	the	DT
cord-345088-krb1eidw	14	44	translocation	translocation	NN
cord-345088-krb1eidw	14	45	of	of	IN
cord-345088-krb1eidw	14	46	the	the	DT
cord-345088-krb1eidw	14	47	protein	protein	NN
cord-345088-krb1eidw	14	48	to	to	IN
cord-345088-krb1eidw	14	49	the	the	DT
cord-345088-krb1eidw	14	50	cell	cell	NN
cord-345088-krb1eidw	14	51	surface	surface	NN
cord-345088-krb1eidw	14	52	.	.	.
cord-345088-krb1eidw	15	1	Consequently	consequently	RB
cord-345088-krb1eidw	15	2	,	,	,
cord-345088-krb1eidw	15	3	some	some	DT
cord-345088-krb1eidw	15	4	essential	essential	JJ
cord-345088-krb1eidw	15	5	functions	function	NNS
cord-345088-krb1eidw	15	6	of	of	IN
cord-345088-krb1eidw	15	7	the	the	DT
cord-345088-krb1eidw	15	8	S	S	NNP
cord-345088-krb1eidw	15	9	protein	protein	NN
cord-345088-krb1eidw	15	10	,	,	,
cord-345088-krb1eidw	15	11	including	include	VBG
cord-345088-krb1eidw	15	12	mediation	mediation	NN
cord-345088-krb1eidw	15	13	of	of	IN
cord-345088-krb1eidw	15	14	cell	cell	NN
cord-345088-krb1eidw	15	15	-	-	HYPH
cord-345088-krb1eidw	15	16	to	to	IN
cord-345088-krb1eidw	15	17	-	-	HYPH
cord-345088-krb1eidw	15	18	cell	cell	NN
cord-345088-krb1eidw	15	19	fusion	fusion	NN
cord-345088-krb1eidw	15	20	and	and	CC
cord-345088-krb1eidw	15	21	its	-PRON-	PRP$
cord-345088-krb1eidw	15	22	incorporation	incorporation	NN
cord-345088-krb1eidw	15	23	into	into	IN
cord-345088-krb1eidw	15	24	virions	virion	NNS
cord-345088-krb1eidw	15	25	,	,	,
cord-345088-krb1eidw	15	26	were	be	VBD
cord-345088-krb1eidw	15	27	completely	completely	RB
cord-345088-krb1eidw	15	28	abolished	abolish	VBN
cord-345088-krb1eidw	15	29	.	.	.
cord-345088-krb1eidw	16	1	The	the	DT
cord-345088-krb1eidw	16	2	Coronaviridae	Coronaviridae	NNP
cord-345088-krb1eidw	16	3	family	family	NN
cord-345088-krb1eidw	16	4	contains	contain	VBZ
cord-345088-krb1eidw	16	5	causative	causative	JJ
cord-345088-krb1eidw	16	6	agents	agent	NNS
cord-345088-krb1eidw	16	7	of	of	IN
cord-345088-krb1eidw	16	8	a	a	DT
cord-345088-krb1eidw	16	9	wide	wide	JJ
cord-345088-krb1eidw	16	10	spectrum	spectrum	NN
cord-345088-krb1eidw	16	11	of	of	IN
cord-345088-krb1eidw	16	12	diseases	disease	NNS
cord-345088-krb1eidw	16	13	affecting	affect	VBG
cord-345088-krb1eidw	16	14	humans	human	NNS
cord-345088-krb1eidw	16	15	,	,	,
cord-345088-krb1eidw	16	16	mammals	mammal	NNS
cord-345088-krb1eidw	16	17	,	,	,
cord-345088-krb1eidw	16	18	and	and	CC
cord-345088-krb1eidw	16	19	birds	bird	NNS
cord-345088-krb1eidw	16	20	.	.	.
cord-345088-krb1eidw	17	1	It	-PRON-	PRP
cord-345088-krb1eidw	17	2	is	be	VBZ
cord-345088-krb1eidw	17	3	the	the	DT
cord-345088-krb1eidw	17	4	etiologic	etiologic	JJ
cord-345088-krb1eidw	17	5	agent	agent	NN
cord-345088-krb1eidw	17	6	of	of	IN
cord-345088-krb1eidw	17	7	severe	severe	JJ
cord-345088-krb1eidw	17	8	acute	acute	JJ
cord-345088-krb1eidw	17	9	respiratory	respiratory	JJ
cord-345088-krb1eidw	17	10	syndrome	syndrome	NN
cord-345088-krb1eidw	17	11	(	(	-LRB-
cord-345088-krb1eidw	17	12	Drosten	Drosten	NNP
cord-345088-krb1eidw	17	13	et	et	FW
cord-345088-krb1eidw	17	14	al	al	NNP
cord-345088-krb1eidw	17	15	.	.	.
cord-345088-krb1eidw	18	1	,	,	,
cord-345088-krb1eidw	18	2	2003	2003	CD
cord-345088-krb1eidw	18	3	;	;	:
cord-345088-krb1eidw	18	4	Fouchier	Fouchier	NNP
cord-345088-krb1eidw	18	5	et	et	NNP
cord-345088-krb1eidw	18	6	al	al	NNP
cord-345088-krb1eidw	18	7	.	.	NNP
cord-345088-krb1eidw	18	8	,	,	,
cord-345088-krb1eidw	18	9	2003	2003	CD
cord-345088-krb1eidw	18	10	)	)	-RRB-
cord-345088-krb1eidw	18	11	.	.	.
cord-345088-krb1eidw	19	1	The	the	DT
cord-345088-krb1eidw	19	2	molecular	molecular	JJ
cord-345088-krb1eidw	19	3	basis	basis	NN
cord-345088-krb1eidw	19	4	of	of	IN
cord-345088-krb1eidw	19	5	host	host	NN
cord-345088-krb1eidw	19	6	specificity	specificity	NN
cord-345088-krb1eidw	19	7	and	and	CC
cord-345088-krb1eidw	19	8	tissue	tissue	NN
cord-345088-krb1eidw	19	9	or	or	CC
cord-345088-krb1eidw	19	10	cell	cell	NN
cord-345088-krb1eidw	19	11	tropism	tropism	NN
cord-345088-krb1eidw	19	12	of	of	IN
cord-345088-krb1eidw	19	13	coronavirus	coronavirus	NN
cord-345088-krb1eidw	19	14	partially	partially	RB
cord-345088-krb1eidw	19	15	resides	reside	VBZ
cord-345088-krb1eidw	19	16	in	in	IN
cord-345088-krb1eidw	19	17	the	the	DT
cord-345088-krb1eidw	19	18	specific	specific	JJ
cord-345088-krb1eidw	19	19	interaction	interaction	NN
cord-345088-krb1eidw	19	20	between	between	IN
cord-345088-krb1eidw	19	21	the	the	DT
cord-345088-krb1eidw	19	22	surface	surface	NN
cord-345088-krb1eidw	19	23	spike	spike	NN
cord-345088-krb1eidw	19	24	protein	protein	NN
cord-345088-krb1eidw	19	25	and	and	CC
cord-345088-krb1eidw	19	26	cellular	cellular	JJ
cord-345088-krb1eidw	19	27	receptor(s	receptor(s	NNP
cord-345088-krb1eidw	19	28	)	)	-RRB-
cord-345088-krb1eidw	19	29	.	.	.
cord-345088-krb1eidw	20	1	This	this	DT
cord-345088-krb1eidw	20	2	interaction	interaction	NN
cord-345088-krb1eidw	20	3	would	would	MD
cord-345088-krb1eidw	20	4	determine	determine	VB
cord-345088-krb1eidw	20	5	the	the	DT
cord-345088-krb1eidw	20	6	outcome	outcome	NN
cord-345088-krb1eidw	20	7	of	of	IN
cord-345088-krb1eidw	20	8	infection	infection	NN
cord-345088-krb1eidw	20	9	:	:	:
cord-345088-krb1eidw	20	10	whether	whether	IN
cord-345088-krb1eidw	20	11	it	-PRON-	PRP
cord-345088-krb1eidw	20	12	is	be	VBZ
cord-345088-krb1eidw	20	13	acute	acute	JJ
cord-345088-krb1eidw	20	14	or	or	CC
cord-345088-krb1eidw	20	15	chronic	chronic	JJ
cord-345088-krb1eidw	20	16	and	and	CC
cord-345088-krb1eidw	20	17	persistent	persistent	JJ
cord-345088-krb1eidw	20	18	(	(	-LRB-
cord-345088-krb1eidw	20	19	Rowe	Rowe	NNP
cord-345088-krb1eidw	20	20	et	et	NNP
cord-345088-krb1eidw	20	21	al	al	NNP
cord-345088-krb1eidw	20	22	.	.	.
cord-345088-krb1eidw	21	1	,	,	,
cord-345088-krb1eidw	21	2	1997a	1997a	CD
cord-345088-krb1eidw	21	3	(	(	-LRB-
cord-345088-krb1eidw	21	4	Rowe	Rowe	NNP
cord-345088-krb1eidw	21	5	et	et	NNP
cord-345088-krb1eidw	21	6	al	al	NNP
cord-345088-krb1eidw	21	7	.	.	.
cord-345088-krb1eidw	22	1	,	,	,
cord-345088-krb1eidw	22	2	,	,	,
cord-345088-krb1eidw	22	3	1997b	1997b	CD
cord-345088-krb1eidw	22	4	,	,	,
cord-345088-krb1eidw	22	5	which	which	WDT
cord-345088-krb1eidw	22	6	organs	organ	NNS
cord-345088-krb1eidw	22	7	or	or	CC
cord-345088-krb1eidw	22	8	tissues	tissue	NNS
cord-345088-krb1eidw	22	9	are	be	VBP
cord-345088-krb1eidw	22	10	targeted	target	VBN
cord-345088-krb1eidw	22	11	,	,	,
cord-345088-krb1eidw	22	12	what	what	WDT
cord-345088-krb1eidw	22	13	symptoms	symptom	NNS
cord-345088-krb1eidw	22	14	are	be	VBP
cord-345088-krb1eidw	22	15	shown	show	VBN
cord-345088-krb1eidw	23	1	(	(	-LRB-
cord-345088-krb1eidw	23	2	Ballesteros	Ballesteros	NNP
cord-345088-krb1eidw	23	3	et	et	NNP
cord-345088-krb1eidw	23	4	al	al	NNP
cord-345088-krb1eidw	23	5	.	.	NNP
cord-345088-krb1eidw	23	6	,	,	,
cord-345088-krb1eidw	23	7	1997	1997	CD
cord-345088-krb1eidw	24	1	;	;	:
cord-345088-krb1eidw	24	2	Leparc	Leparc	NNP
cord-345088-krb1eidw	24	3	-	-	HYPH
cord-345088-krb1eidw	24	4	Goffart	Goffart	NNP
cord-345088-krb1eidw	24	5	et	et	NNP
cord-345088-krb1eidw	24	6	al	al	NNP
cord-345088-krb1eidw	24	7	.	.	NNP
cord-345088-krb1eidw	24	8	,	,	,
cord-345088-krb1eidw	24	9	1997	1997	CD
cord-345088-krb1eidw	24	10	;	;	:
cord-345088-krb1eidw	24	11	Navas	Navas	NNP
cord-345088-krb1eidw	24	12	et	et	NNP
cord-345088-krb1eidw	24	13	al	al	NNP
cord-345088-krb1eidw	24	14	.	.	NNP
cord-345088-krb1eidw	24	15	,	,	,
cord-345088-krb1eidw	24	16	2001	2001	CD
cord-345088-krb1eidw	24	17	)	)	-RRB-
cord-345088-krb1eidw	24	18	,	,	,
cord-345088-krb1eidw	24	19	and	and	CC
cord-345088-krb1eidw	24	20	even	even	RB
cord-345088-krb1eidw	24	21	whether	whether	IN
cord-345088-krb1eidw	24	22	host	host	NN
cord-345088-krb1eidw	24	23	specificity	specificity	NN
cord-345088-krb1eidw	24	24	is	be	VBZ
cord-345088-krb1eidw	24	25	changed	change	VBN
cord-345088-krb1eidw	25	1	(	(	-LRB-
cord-345088-krb1eidw	25	2	Kuo	Kuo	NNP
cord-345088-krb1eidw	25	3	et	et	NNP
cord-345088-krb1eidw	25	4	al	al	NNP
cord-345088-krb1eidw	25	5	.	.	NNP
cord-345088-krb1eidw	25	6	,	,	,
cord-345088-krb1eidw	25	7	2000	2000	CD
cord-345088-krb1eidw	25	8	)	)	-RRB-
cord-345088-krb1eidw	25	9	.	.	.
cord-345088-krb1eidw	26	1	Coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	26	2	are	be	VBP
cord-345088-krb1eidw	26	3	a	a	DT
cord-345088-krb1eidw	26	4	group	group	NN
cord-345088-krb1eidw	26	5	of	of	IN
cord-345088-krb1eidw	26	6	enveloped	envelop	VBN
cord-345088-krb1eidw	26	7	RNA	RNA	NNP
cord-345088-krb1eidw	26	8	viruses	virus	NNS
cord-345088-krb1eidw	26	9	with	with	IN
cord-345088-krb1eidw	26	10	positive	positive	JJ
cord-345088-krb1eidw	26	11	-	-	HYPH
cord-345088-krb1eidw	26	12	sense	sense	NN
cord-345088-krb1eidw	26	13	,	,	,
cord-345088-krb1eidw	26	14	single	single	RB
cord-345088-krb1eidw	26	15	-	-	HYPH
cord-345088-krb1eidw	26	16	stranded	strand	VBN
cord-345088-krb1eidw	26	17	genomes	genome	NNS
cord-345088-krb1eidw	26	18	of	of	IN
cord-345088-krb1eidw	26	19	27	27	CD
cord-345088-krb1eidw	26	20	to	to	TO
cord-345088-krb1eidw	26	21	32	32	CD
cord-345088-krb1eidw	26	22	kb	kb	NNS
cord-345088-krb1eidw	26	23	that	that	WDT
cord-345088-krb1eidw	26	24	are	be	VBP
cord-345088-krb1eidw	26	25	packaged	package	VBN
cord-345088-krb1eidw	26	26	into	into	IN
cord-345088-krb1eidw	26	27	helical	helical	JJ
cord-345088-krb1eidw	26	28	structures	structure	NNS
cord-345088-krb1eidw	26	29	by	by	IN
cord-345088-krb1eidw	26	30	the	the	DT
cord-345088-krb1eidw	26	31	nucleocapsid	nucleocapsid	NNP
cord-345088-krb1eidw	26	32	protein	protein	NNP
cord-345088-krb1eidw	26	33	(	(	-LRB-
cord-345088-krb1eidw	26	34	N	N	NNP
cord-345088-krb1eidw	26	35	)	)	-RRB-
cord-345088-krb1eidw	26	36	.	.	.
cord-345088-krb1eidw	27	1	The	the	DT
cord-345088-krb1eidw	27	2	viruses	virus	NNS
cord-345088-krb1eidw	27	3	acquire	acquire	VBP
cord-345088-krb1eidw	27	4	their	-PRON-	PRP$
cord-345088-krb1eidw	27	5	envelopes	envelope	NNS
cord-345088-krb1eidw	27	6	by	by	IN
cord-345088-krb1eidw	27	7	budding	bud	VBG
cord-345088-krb1eidw	27	8	of	of	IN
cord-345088-krb1eidw	27	9	the	the	DT
cord-345088-krb1eidw	27	10	structural	structural	JJ
cord-345088-krb1eidw	27	11	components	component	NNS
cord-345088-krb1eidw	27	12	into	into	IN
cord-345088-krb1eidw	27	13	the	the	DT
cord-345088-krb1eidw	27	14	intermediate	intermediate	JJ
cord-345088-krb1eidw	27	15	compartment	compartment	NN
cord-345088-krb1eidw	27	16	(	(	-LRB-
cord-345088-krb1eidw	27	17	IC	IC	NNP
cord-345088-krb1eidw	27	18	)	)	-RRB-
cord-345088-krb1eidw	27	19	between	between	IN
cord-345088-krb1eidw	27	20	the	the	DT
cord-345088-krb1eidw	27	21	endoplasmic	endoplasmic	NN
cord-345088-krb1eidw	27	22	reticulum	reticulum	NN
cord-345088-krb1eidw	27	23	(	(	-LRB-
cord-345088-krb1eidw	27	24	ER	ER	NNP
cord-345088-krb1eidw	27	25	)	)	-RRB-
cord-345088-krb1eidw	27	26	and	and	CC
cord-345088-krb1eidw	27	27	the	the	DT
cord-345088-krb1eidw	27	28	Golgi	Golgi	NNP
cord-345088-krb1eidw	27	29	apparatus	apparatus	NN
cord-345088-krb1eidw	27	30	(	(	-LRB-
cord-345088-krb1eidw	27	31	Krijnse	Krijnse	NNP
cord-345088-krb1eidw	27	32	-	-	HYPH
cord-345088-krb1eidw	27	33	Locker	Locker	NNP
cord-345088-krb1eidw	27	34	et	et	FW
cord-345088-krb1eidw	27	35	al	al	NNP
cord-345088-krb1eidw	27	36	.	.	NNP
cord-345088-krb1eidw	27	37	,	,	,
cord-345088-krb1eidw	27	38	1994	1994	CD
cord-345088-krb1eidw	27	39	;	;	:
cord-345088-krb1eidw	27	40	Lai	Lai	NNP
cord-345088-krb1eidw	27	41	and	and	CC
cord-345088-krb1eidw	27	42	Cavanagh	Cavanagh	NNP
cord-345088-krb1eidw	27	43	,	,	,
cord-345088-krb1eidw	27	44	1998	1998	CD
cord-345088-krb1eidw	27	45	;	;	:
cord-345088-krb1eidw	27	46	Tooze	Tooze	NNP
cord-345088-krb1eidw	27	47	et	et	NNP
cord-345088-krb1eidw	27	48	al	al	NNP
cord-345088-krb1eidw	27	49	.	.	NNP
cord-345088-krb1eidw	27	50	,	,	,
cord-345088-krb1eidw	27	51	1984	1984	CD
cord-345088-krb1eidw	27	52	)	)	-RRB-
cord-345088-krb1eidw	27	53	.	.	.
cord-345088-krb1eidw	28	1	The	the	DT
cord-345088-krb1eidw	28	2	membrane	membrane	NN
cord-345088-krb1eidw	28	3	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	28	4	(	(	-LRB-
cord-345088-krb1eidw	28	5	M	M	NNP
cord-345088-krb1eidw	28	6	)	)	-RRB-
cord-345088-krb1eidw	28	7	and	and	CC
cord-345088-krb1eidw	28	8	the	the	DT
cord-345088-krb1eidw	28	9	envelope	envelope	NN
cord-345088-krb1eidw	28	10	protein	protein	NN
cord-345088-krb1eidw	28	11	(	(	-LRB-
cord-345088-krb1eidw	28	12	E	e	NN
cord-345088-krb1eidw	28	13	)	)	-RRB-
cord-345088-krb1eidw	28	14	mediate	mediate	VB
cord-345088-krb1eidw	28	15	the	the	DT
cord-345088-krb1eidw	28	16	formation	formation	NN
cord-345088-krb1eidw	28	17	of	of	IN
cord-345088-krb1eidw	28	18	the	the	DT
cord-345088-krb1eidw	28	19	envelope	envelope	NN
cord-345088-krb1eidw	28	20	,	,	,
cord-345088-krb1eidw	28	21	as	as	IN
cord-345088-krb1eidw	28	22	they	-PRON-	PRP
cord-345088-krb1eidw	28	23	are	be	VBP
cord-345088-krb1eidw	28	24	involved	involve	VBN
cord-345088-krb1eidw	28	25	in	in	IN
cord-345088-krb1eidw	28	26	the	the	DT
cord-345088-krb1eidw	28	27	induction	induction	NN
cord-345088-krb1eidw	28	28	of	of	IN
cord-345088-krb1eidw	28	29	virion	virion	NN
cord-345088-krb1eidw	28	30	assembly	assembly	NN
cord-345088-krb1eidw	28	31	and	and	CC
cord-345088-krb1eidw	28	32	incorporation	incorporation	NN
cord-345088-krb1eidw	28	33	of	of	IN
cord-345088-krb1eidw	28	34	other	other	JJ
cord-345088-krb1eidw	28	35	structural	structural	JJ
cord-345088-krb1eidw	28	36	components	component	NNS
cord-345088-krb1eidw	28	37	into	into	IN
cord-345088-krb1eidw	28	38	virus	virus	NN
cord-345088-krb1eidw	28	39	particles	particle	NNS
cord-345088-krb1eidw	28	40	(	(	-LRB-
cord-345088-krb1eidw	28	41	Vennema	Vennema	NNP
cord-345088-krb1eidw	28	42	et	et	FW
cord-345088-krb1eidw	28	43	al	al	NNP
cord-345088-krb1eidw	28	44	.	.	NNP
cord-345088-krb1eidw	28	45	,	,	,
cord-345088-krb1eidw	28	46	1996	1996	CD
cord-345088-krb1eidw	28	47	)	)	-RRB-
cord-345088-krb1eidw	28	48	.	.	.
cord-345088-krb1eidw	29	1	The	the	DT
cord-345088-krb1eidw	29	2	spike	spike	NN
cord-345088-krb1eidw	29	3	(	(	-LRB-
cord-345088-krb1eidw	29	4	S	s	NN
cord-345088-krb1eidw	29	5	)	)	-RRB-
cord-345088-krb1eidw	29	6	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	29	7	incorporate	incorporate	VBP
cord-345088-krb1eidw	29	8	into	into	IN
cord-345088-krb1eidw	29	9	the	the	DT
cord-345088-krb1eidw	29	10	envelope	envelope	NN
cord-345088-krb1eidw	29	11	(	(	-LRB-
cord-345088-krb1eidw	29	12	de	de	FW
cord-345088-krb1eidw	29	13	Haan	Haan	NNP
cord-345088-krb1eidw	29	14	et	et	FW
cord-345088-krb1eidw	29	15	al	al	NNP
cord-345088-krb1eidw	29	16	.	.	NNP
cord-345088-krb1eidw	29	17	,	,	,
cord-345088-krb1eidw	29	18	1999	1999	CD
cord-345088-krb1eidw	29	19	)	)	-RRB-
cord-345088-krb1eidw	29	20	in	in	IN
cord-345088-krb1eidw	29	21	the	the	DT
cord-345088-krb1eidw	29	22	form	form	NN
cord-345088-krb1eidw	29	23	of	of	IN
cord-345088-krb1eidw	29	24	homooligomeric	homooligomeric	NNP
cord-345088-krb1eidw	29	25	,	,	,
cord-345088-krb1eidw	29	26	20	20	CD
cord-345088-krb1eidw	29	27	nm	nm	NN
cord-345088-krb1eidw	29	28	projections	projection	NNS
cord-345088-krb1eidw	29	29	,	,	,
cord-345088-krb1eidw	29	30	giving	give	VBG
cord-345088-krb1eidw	29	31	the	the	DT
cord-345088-krb1eidw	29	32	distinctive	distinctive	JJ
cord-345088-krb1eidw	29	33	morphology	morphology	NN
cord-345088-krb1eidw	29	34	of	of	IN
cord-345088-krb1eidw	29	35	the	the	DT
cord-345088-krb1eidw	29	36	coronavirus	coronavirus	NN
cord-345088-krb1eidw	29	37	virion	virion	NN
cord-345088-krb1eidw	29	38	.	.	.
cord-345088-krb1eidw	30	1	Some	some	DT
cord-345088-krb1eidw	30	2	coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	30	3	also	also	RB
cord-345088-krb1eidw	30	4	contain	contain	VBP
cord-345088-krb1eidw	30	5	another	another	DT
cord-345088-krb1eidw	30	6	envelope	envelope	NN
cord-345088-krb1eidw	30	7	protein	protein	NN
cord-345088-krb1eidw	30	8	,	,	,
cord-345088-krb1eidw	30	9	the	the	DT
cord-345088-krb1eidw	30	10	hemagglutinin	hemagglutinin	NN
cord-345088-krb1eidw	30	11	esterase	esterase	NN
cord-345088-krb1eidw	30	12	(	(	-LRB-
cord-345088-krb1eidw	30	13	HE	HE	NNP
cord-345088-krb1eidw	30	14	)	)	-RRB-
cord-345088-krb1eidw	30	15	.	.	.
cord-345088-krb1eidw	31	1	The	the	DT
cord-345088-krb1eidw	31	2	largest	large	JJS
cord-345088-krb1eidw	31	3	structural	structural	JJ
cord-345088-krb1eidw	31	4	S	s	NN
cord-345088-krb1eidw	31	5	protein	protein	NN
cord-345088-krb1eidw	31	6	is	be	VBZ
cord-345088-krb1eidw	31	7	a	a	DT
cord-345088-krb1eidw	31	8	type	type	NN
cord-345088-krb1eidw	31	9	I	-PRON-	PRP
cord-345088-krb1eidw	31	10	integral	integral	JJ
cord-345088-krb1eidw	31	11	membrane	membrane	NN
cord-345088-krb1eidw	31	12	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	31	13	and	and	CC
cord-345088-krb1eidw	31	14	spans	span	VBZ
cord-345088-krb1eidw	31	15	the	the	DT
cord-345088-krb1eidw	31	16	viral	viral	JJ
cord-345088-krb1eidw	31	17	envelope	envelope	NN
cord-345088-krb1eidw	31	18	once	once	RB
cord-345088-krb1eidw	31	19	.	.	.
cord-345088-krb1eidw	32	1	In	in	IN
cord-345088-krb1eidw	32	2	some	some	DT
cord-345088-krb1eidw	32	3	cases	case	NNS
cord-345088-krb1eidw	32	4	,	,	,
cord-345088-krb1eidw	32	5	the	the	DT
cord-345088-krb1eidw	32	6	S	S	NNP
cord-345088-krb1eidw	32	7	protein	protein	NN
cord-345088-krb1eidw	32	8	is	be	VBZ
cord-345088-krb1eidw	32	9	proteolytically	proteolytically	RB
cord-345088-krb1eidw	32	10	cleaved	cleave	VBN
cord-345088-krb1eidw	32	11	into	into	IN
cord-345088-krb1eidw	32	12	the	the	DT
cord-345088-krb1eidw	32	13	N	n	CD
cord-345088-krb1eidw	32	14	-	-	HYPH
cord-345088-krb1eidw	32	15	terminal	terminal	NN
cord-345088-krb1eidw	32	16	S1	s1	NN
cord-345088-krb1eidw	32	17	and	and	CC
cord-345088-krb1eidw	32	18	the	the	DT
cord-345088-krb1eidw	32	19	C	C	NNP
cord-345088-krb1eidw	32	20	-	-	HYPH
cord-345088-krb1eidw	32	21	terminal	terminal	NN
cord-345088-krb1eidw	32	22	S2	s2	NN
cord-345088-krb1eidw	32	23	subunits	subunit	NNS
cord-345088-krb1eidw	32	24	of	of	IN
cord-345088-krb1eidw	32	25	equal	equal	JJ
cord-345088-krb1eidw	32	26	size	size	NN
cord-345088-krb1eidw	32	27	by	by	IN
cord-345088-krb1eidw	32	28	a	a	DT
cord-345088-krb1eidw	32	29	host	host	NN
cord-345088-krb1eidw	32	30	proteinase	proteinase	NN
cord-345088-krb1eidw	32	31	.	.	.
cord-345088-krb1eidw	33	1	The	the	DT
cord-345088-krb1eidw	33	2	S1	S1	NNP
cord-345088-krb1eidw	33	3	and	and	CC
cord-345088-krb1eidw	33	4	S2	s2	NN
cord-345088-krb1eidw	33	5	subunits	subunit	NNS
cord-345088-krb1eidw	33	6	are	be	VBP
cord-345088-krb1eidw	33	7	noncovalently	noncovalently	RB
cord-345088-krb1eidw	33	8	associated	associate	VBN
cord-345088-krb1eidw	33	9	to	to	IN
cord-345088-krb1eidw	33	10	each	each	DT
cord-345088-krb1eidw	33	11	other	other	JJ
cord-345088-krb1eidw	33	12	.	.	.
cord-345088-krb1eidw	34	1	A	a	DT
cord-345088-krb1eidw	34	2	hydrophobic	hydrophobic	JJ
cord-345088-krb1eidw	34	3	transmembrane	transmembrane	NN
cord-345088-krb1eidw	34	4	domain	domain	NN
cord-345088-krb1eidw	34	5	(	(	-LRB-
cord-345088-krb1eidw	34	6	TM	TM	NNP
cord-345088-krb1eidw	34	7	)	)	-RRB-
cord-345088-krb1eidw	34	8	of	of	IN
cord-345088-krb1eidw	34	9	S2	S2	NNP
cord-345088-krb1eidw	34	10	anchors	anchor	NNS
cord-345088-krb1eidw	34	11	on	on	IN
cord-345088-krb1eidw	34	12	the	the	DT
cord-345088-krb1eidw	34	13	envelope	envelope	NN
cord-345088-krb1eidw	34	14	with	with	IN
cord-345088-krb1eidw	34	15	a	a	DT
cord-345088-krb1eidw	34	16	short	short	JJ
cord-345088-krb1eidw	34	17	cytoplasmic	cytoplasmic	JJ
cord-345088-krb1eidw	34	18	-	-	HYPH
cord-345088-krb1eidw	34	19	tail	tail	NN
cord-345088-krb1eidw	34	20	inside	inside	RB
cord-345088-krb1eidw	34	21	.	.	.
cord-345088-krb1eidw	35	1	One	one	CD
cord-345088-krb1eidw	35	2	Leucine	leucine	NN
cord-345088-krb1eidw	35	3	-	-	HYPH
cord-345088-krb1eidw	35	4	zipper	zipper	NN
cord-345088-krb1eidw	35	5	(	(	-LRB-
cord-345088-krb1eidw	35	6	heptad	heptad	NNP
cord-345088-krb1eidw	35	7	repeats	repeat	VBZ
cord-345088-krb1eidw	35	8	)	)	-RRB-
cord-345088-krb1eidw	35	9	domain	domain	NN
cord-345088-krb1eidw	35	10	overlaps	overlap	VBZ
cord-345088-krb1eidw	35	11	with	with	IN
cord-345088-krb1eidw	35	12	the	the	DT
cord-345088-krb1eidw	35	13	TM	TM	NNP
cord-345088-krb1eidw	35	14	domain	domain	NN
cord-345088-krb1eidw	35	15	and	and	CC
cord-345088-krb1eidw	35	16	another	another	DT
cord-345088-krb1eidw	35	17	extends	extend	VBZ
cord-345088-krb1eidw	35	18	outside	outside	IN
cord-345088-krb1eidw	35	19	the	the	DT
cord-345088-krb1eidw	35	20	envelope	envelope	NN
cord-345088-krb1eidw	35	21	,	,	,
cord-345088-krb1eidw	35	22	folding	fold	VBG
cord-345088-krb1eidw	35	23	into	into	IN
cord-345088-krb1eidw	35	24	a	a	DT
cord-345088-krb1eidw	35	25	-	-	:
cord-345088-krb1eidw	35	26	helices	helix	NNS
cord-345088-krb1eidw	35	27	and	and	CC
cord-345088-krb1eidw	35	28	coiled	coil	VBN
cord-345088-krb1eidw	35	29	-	-	HYPH
cord-345088-krb1eidw	35	30	coil	coil	NN
cord-345088-krb1eidw	35	31	structures	structure	NNS
cord-345088-krb1eidw	35	32	involved	involve	VBN
cord-345088-krb1eidw	35	33	in	in	IN
cord-345088-krb1eidw	35	34	oligomerization	oligomerization	NN
cord-345088-krb1eidw	35	35	(	(	-LRB-
cord-345088-krb1eidw	35	36	de	de	FW
cord-345088-krb1eidw	35	37	Groot	Groot	NNP
cord-345088-krb1eidw	35	38	et	et	FW
cord-345088-krb1eidw	35	39	al	al	NNP
cord-345088-krb1eidw	35	40	.	.	NNP
cord-345088-krb1eidw	35	41	,	,	,
cord-345088-krb1eidw	35	42	1987	1987	CD
cord-345088-krb1eidw	35	43	)	)	-RRB-
cord-345088-krb1eidw	35	44	.	.	.
cord-345088-krb1eidw	36	1	It	-PRON-	PRP
cord-345088-krb1eidw	36	2	was	be	VBD
cord-345088-krb1eidw	36	3	found	find	VBN
cord-345088-krb1eidw	36	4	that	that	IN
cord-345088-krb1eidw	36	5	the	the	DT
cord-345088-krb1eidw	36	6	TM	TM	NNP
cord-345088-krb1eidw	36	7	domain	domain	NN
cord-345088-krb1eidw	36	8	and	and	CC
cord-345088-krb1eidw	36	9	endodomain	endodomain	NN
cord-345088-krb1eidw	36	10	mediated	mediate	VBD
cord-345088-krb1eidw	36	11	the	the	DT
cord-345088-krb1eidw	36	12	incorporation	incorporation	NN
cord-345088-krb1eidw	36	13	of	of	IN
cord-345088-krb1eidw	36	14	S	S	NNP
cord-345088-krb1eidw	36	15	into	into	IN
cord-345088-krb1eidw	36	16	virions	virion	NNS
cord-345088-krb1eidw	36	17	but	but	CC
cord-345088-krb1eidw	36	18	the	the	DT
cord-345088-krb1eidw	36	19	details	detail	NNS
cord-345088-krb1eidw	36	20	of	of	IN
cord-345088-krb1eidw	36	21	the	the	DT
cord-345088-krb1eidw	36	22	S	S	NNP
cord-345088-krb1eidw	36	23	/	/	SYM
cord-345088-krb1eidw	36	24	M	M	NNP
cord-345088-krb1eidw	36	25	interaction	interaction	NN
cord-345088-krb1eidw	36	26	remain	remain	VBP
cord-345088-krb1eidw	36	27	to	to	TO
cord-345088-krb1eidw	36	28	be	be	VB
cord-345088-krb1eidw	36	29	elucidated	elucidate	VBN
cord-345088-krb1eidw	36	30	.	.	.
cord-345088-krb1eidw	37	1	Although	although	IN
cord-345088-krb1eidw	37	2	S	S	NNP
cord-345088-krb1eidw	37	3	protein	protein	NN
cord-345088-krb1eidw	37	4	is	be	VBZ
cord-345088-krb1eidw	37	5	dispensable	dispensable	JJ
cord-345088-krb1eidw	37	6	for	for	IN
cord-345088-krb1eidw	37	7	the	the	DT
cord-345088-krb1eidw	37	8	formation	formation	NN
cord-345088-krb1eidw	37	9	of	of	IN
cord-345088-krb1eidw	37	10	virus	virus	NN
cord-345088-krb1eidw	37	11	-	-	HYPH
cord-345088-krb1eidw	37	12	like	like	JJ
cord-345088-krb1eidw	37	13	-	-	HYPH
cord-345088-krb1eidw	37	14	particles	particle	NNS
cord-345088-krb1eidw	37	15	(	(	-LRB-
cord-345088-krb1eidw	37	16	VLPs	VLPs	NNP
cord-345088-krb1eidw	37	17	)	)	-RRB-
cord-345088-krb1eidw	37	18	,	,	,
cord-345088-krb1eidw	37	19	its	-PRON-	PRP$
cord-345088-krb1eidw	37	20	incorporation	incorporation	NN
cord-345088-krb1eidw	37	21	into	into	IN
cord-345088-krb1eidw	37	22	virion	virion	NN
cord-345088-krb1eidw	37	23	is	be	VBZ
cord-345088-krb1eidw	37	24	critical	critical	JJ
cord-345088-krb1eidw	37	25	to	to	IN
cord-345088-krb1eidw	37	26	the	the	DT
cord-345088-krb1eidw	37	27	assembly	assembly	NN
cord-345088-krb1eidw	37	28	of	of	IN
cord-345088-krb1eidw	37	29	infectious	infectious	JJ
cord-345088-krb1eidw	37	30	virus	virus	NN
cord-345088-krb1eidw	37	31	particles	particle	NNS
cord-345088-krb1eidw	37	32	.	.	.
cord-345088-krb1eidw	38	1	The	the	DT
cord-345088-krb1eidw	38	2	S	S	NNP
cord-345088-krb1eidw	38	3	protein	protein	NN
cord-345088-krb1eidw	38	4	is	be	VBZ
cord-345088-krb1eidw	38	5	cotranslationally	cotranslationally	RB
cord-345088-krb1eidw	38	6	N	n	CD
cord-345088-krb1eidw	38	7	-	-	HYPH
cord-345088-krb1eidw	38	8	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	38	9	in	in	IN
cord-345088-krb1eidw	38	10	the	the	DT
cord-345088-krb1eidw	38	11	ER	er	NN
cord-345088-krb1eidw	38	12	and	and	CC
cord-345088-krb1eidw	38	13	also	also	RB
cord-345088-krb1eidw	38	14	forms	form	VBZ
cord-345088-krb1eidw	38	15	stable	stable	JJ
cord-345088-krb1eidw	38	16	complex	complex	NN
cord-345088-krb1eidw	38	17	with	with	IN
cord-345088-krb1eidw	38	18	M	M	NNP
cord-345088-krb1eidw	38	19	in	in	IN
cord-345088-krb1eidw	38	20	the	the	DT
cord-345088-krb1eidw	38	21	pre	pre	JJ
cord-345088-krb1eidw	38	22	-	-	JJ
cord-345088-krb1eidw	38	23	Golgi	golgi	JJ
cord-345088-krb1eidw	38	24	membrane	membrane	NN
cord-345088-krb1eidw	39	1	(	(	-LRB-
cord-345088-krb1eidw	39	2	Holmes	Holmes	NNP
cord-345088-krb1eidw	39	3	et	et	NNP
cord-345088-krb1eidw	39	4	al	al	NNP
cord-345088-krb1eidw	39	5	.	.	.
cord-345088-krb1eidw	40	1	,	,	,
cord-345088-krb1eidw	40	2	1981	1981	CD
cord-345088-krb1eidw	40	3	;	;	:
cord-345088-krb1eidw	40	4	Opstelten	Opstelten	NNP
cord-345088-krb1eidw	40	5	et	et	NNP
cord-345088-krb1eidw	40	6	al	al	NNP
cord-345088-krb1eidw	40	7	.	.	NNP
cord-345088-krb1eidw	40	8	,	,	,
cord-345088-krb1eidw	40	9	1995	1995	CD
cord-345088-krb1eidw	40	10	;	;	:
cord-345088-krb1eidw	40	11	Sturman	Sturman	NNP
cord-345088-krb1eidw	40	12	et	et	NNP
cord-345088-krb1eidw	40	13	al	al	NNP
cord-345088-krb1eidw	40	14	.	.	NNP
cord-345088-krb1eidw	40	15	,	,	,
cord-345088-krb1eidw	40	16	1985	1985	CD
cord-345088-krb1eidw	40	17	)	)	-RRB-
cord-345088-krb1eidw	40	18	.	.	.
cord-345088-krb1eidw	41	1	It	-PRON-	PRP
cord-345088-krb1eidw	41	2	was	be	VBD
cord-345088-krb1eidw	41	3	then	then	RB
cord-345088-krb1eidw	41	4	transported	transport	VBN
cord-345088-krb1eidw	41	5	to	to	IN
cord-345088-krb1eidw	41	6	the	the	DT
cord-345088-krb1eidw	41	7	Golgi	Golgi	NNP
cord-345088-krb1eidw	41	8	apparatus	apparatus	NN
cord-345088-krb1eidw	41	9	where	where	WRB
cord-345088-krb1eidw	41	10	its	-PRON-	PRP$
cord-345088-krb1eidw	41	11	high	high	JJ
cord-345088-krb1eidw	41	12	mannose	mannose	NN
cord-345088-krb1eidw	41	13	side	side	NN
cord-345088-krb1eidw	41	14	chains	chain	NNS
cord-345088-krb1eidw	41	15	are	be	VBP
cord-345088-krb1eidw	41	16	subsequently	subsequently	RB
cord-345088-krb1eidw	41	17	trimmed	trim	VBN
cord-345088-krb1eidw	41	18	and	and	CC
cord-345088-krb1eidw	41	19	further	further	RB
cord-345088-krb1eidw	41	20	modified	modify	VBN
cord-345088-krb1eidw	41	21	.	.	.
cord-345088-krb1eidw	42	1	The	the	DT
cord-345088-krb1eidw	42	2	matured	matured	JJ
cord-345088-krb1eidw	42	3	S	S	NNP
cord-345088-krb1eidw	42	4	protein	protein	NN
cord-345088-krb1eidw	42	5	is	be	VBZ
cord-345088-krb1eidw	42	6	an	an	DT
cord-345088-krb1eidw	42	7	Endo	endo	NN
cord-345088-krb1eidw	42	8	-	-	HYPH
cord-345088-krb1eidw	42	9	glycosidase	glycosidase	NN
cord-345088-krb1eidw	42	10	-	-	HYPH
cord-345088-krb1eidw	42	11	H	h	NN
cord-345088-krb1eidw	42	12	-	-	HYPH
cord-345088-krb1eidw	42	13	resistant	resistant	JJ
cord-345088-krb1eidw	42	14	(	(	-LRB-
cord-345088-krb1eidw	42	15	Endo	endo	NN
cord-345088-krb1eidw	42	16	-	-	HYPH
cord-345088-krb1eidw	42	17	H	H	NNP
cord-345088-krb1eidw	42	18	)	)	-RRB-
cord-345088-krb1eidw	42	19	,	,	,
cord-345088-krb1eidw	42	20	180-kDa	180-kda	CD
cord-345088-krb1eidw	42	21	form	form	NN
cord-345088-krb1eidw	42	22	(	(	-LRB-
cord-345088-krb1eidw	42	23	Luytjes	Luytjes	NNP
cord-345088-krb1eidw	42	24	et	et	NNP
cord-345088-krb1eidw	42	25	al	al	NNP
cord-345088-krb1eidw	42	26	.	.	NNP
cord-345088-krb1eidw	42	27	,	,	,
cord-345088-krb1eidw	42	28	1997	1997	CD
cord-345088-krb1eidw	42	29	)	)	-RRB-
cord-345088-krb1eidw	42	30	.	.	.
cord-345088-krb1eidw	43	1	At	at	IN
cord-345088-krb1eidw	43	2	the	the	DT
cord-345088-krb1eidw	43	3	trans	trans	NNP
cord-345088-krb1eidw	43	4	-	-	NNP
cord-345088-krb1eidw	43	5	Golgi	Golgi	NNP
cord-345088-krb1eidw	43	6	,	,	,
cord-345088-krb1eidw	43	7	the	the	DT
cord-345088-krb1eidw	43	8	matured	mature	VBN
cord-345088-krb1eidw	43	9	virions	virion	NNS
cord-345088-krb1eidw	43	10	are	be	VBP
cord-345088-krb1eidw	43	11	encapsulated	encapsulate	VBN
cord-345088-krb1eidw	43	12	into	into	IN
cord-345088-krb1eidw	43	13	vesicles	vesicle	NNS
cord-345088-krb1eidw	43	14	of	of	IN
cord-345088-krb1eidw	43	15	the	the	DT
cord-345088-krb1eidw	43	16	constitutive	constitutive	JJ
cord-345088-krb1eidw	43	17	secretory	secretory	JJ
cord-345088-krb1eidw	43	18	pathway	pathway	NN
cord-345088-krb1eidw	43	19	and	and	CC
cord-345088-krb1eidw	43	20	released	release	VBN
cord-345088-krb1eidw	43	21	from	from	IN
cord-345088-krb1eidw	43	22	infected	infected	JJ
cord-345088-krb1eidw	43	23	cells	cell	NNS
cord-345088-krb1eidw	43	24	.	.	.
cord-345088-krb1eidw	44	1	A	a	DT
cord-345088-krb1eidw	44	2	portion	portion	NN
cord-345088-krb1eidw	44	3	of	of	IN
cord-345088-krb1eidw	44	4	S	s	NN
cord-345088-krb1eidw	44	5	protein	protein	NN
cord-345088-krb1eidw	44	6	might	may	MD
cord-345088-krb1eidw	44	7	be	be	VB
cord-345088-krb1eidw	44	8	transported	transport	VBN
cord-345088-krb1eidw	44	9	to	to	IN
cord-345088-krb1eidw	44	10	the	the	DT
cord-345088-krb1eidw	44	11	plasma	plasma	NN
cord-345088-krb1eidw	44	12	membrane	membrane	NN
cord-345088-krb1eidw	44	13	and	and	CC
cord-345088-krb1eidw	44	14	responsible	responsible	JJ
cord-345088-krb1eidw	44	15	for	for	IN
cord-345088-krb1eidw	44	16	the	the	DT
cord-345088-krb1eidw	44	17	cell	cell	NN
cord-345088-krb1eidw	44	18	-	-	HYPH
cord-345088-krb1eidw	44	19	to	to	IN
cord-345088-krb1eidw	44	20	-	-	HYPH
cord-345088-krb1eidw	44	21	cell	cell	NN
cord-345088-krb1eidw	44	22	membrane	membrane	NN
cord-345088-krb1eidw	44	23	fusion	fusion	NN
cord-345088-krb1eidw	44	24	,	,	,
cord-345088-krb1eidw	44	25	resulting	result	VBG
cord-345088-krb1eidw	44	26	in	in	IN
cord-345088-krb1eidw	44	27	the	the	DT
cord-345088-krb1eidw	44	28	formation	formation	NN
cord-345088-krb1eidw	44	29	of	of	IN
cord-345088-krb1eidw	44	30	typical	typical	JJ
cord-345088-krb1eidw	44	31	syncycia	syncycia	NNS
cord-345088-krb1eidw	44	32	and	and	CC
cord-345088-krb1eidw	44	33	spread	spread	NN
cord-345088-krb1eidw	44	34	of	of	IN
cord-345088-krb1eidw	44	35	virus	virus	NN
cord-345088-krb1eidw	44	36	infection	infection	NN
cord-345088-krb1eidw	44	37	to	to	IN
cord-345088-krb1eidw	44	38	neighboring	neighboring	NN
cord-345088-krb1eidw	44	39	cells	cell	NNS
cord-345088-krb1eidw	44	40	.	.	.
cord-345088-krb1eidw	45	1	The	the	DT
cord-345088-krb1eidw	45	2	TM	TM	NNP
cord-345088-krb1eidw	45	3	,	,	,
cord-345088-krb1eidw	45	4	Cysrich	Cysrich	NNP
cord-345088-krb1eidw	45	5	and	and	CC
cord-345088-krb1eidw	45	6	Leucine	Leucine	NNP
cord-345088-krb1eidw	45	7	-	-	HYPH
cord-345088-krb1eidw	45	8	zipper	zipper	NN
cord-345088-krb1eidw	45	9	domains	domain	NNS
cord-345088-krb1eidw	45	10	of	of	IN
cord-345088-krb1eidw	45	11	S2	s2	NN
cord-345088-krb1eidw	45	12	and	and	CC
cord-345088-krb1eidw	45	13	the	the	DT
cord-345088-krb1eidw	45	14	disparate	disparate	JJ
cord-345088-krb1eidw	45	15	region	region	NN
cord-345088-krb1eidw	45	16	of	of	IN
cord-345088-krb1eidw	45	17	S1	S1	NNP
cord-345088-krb1eidw	45	18	are	be	VBP
cord-345088-krb1eidw	45	19	associated	associate	VBN
cord-345088-krb1eidw	45	20	with	with	IN
cord-345088-krb1eidw	45	21	the	the	DT
cord-345088-krb1eidw	45	22	membrane	membrane	NN
cord-345088-krb1eidw	45	23	fusion	fusion	NN
cord-345088-krb1eidw	45	24	activity	activity	NN
cord-345088-krb1eidw	45	25	of	of	IN
cord-345088-krb1eidw	45	26	the	the	DT
cord-345088-krb1eidw	45	27	protein	protein	NN
cord-345088-krb1eidw	46	1	(	(	-LRB-
cord-345088-krb1eidw	46	2	Chang	Chang	NNP
cord-345088-krb1eidw	46	3	et	et	NNP
cord-345088-krb1eidw	46	4	al	al	NNP
cord-345088-krb1eidw	46	5	.	.	.
cord-345088-krb1eidw	47	1	,	,	,
cord-345088-krb1eidw	47	2	2000	2000	CD
cord-345088-krb1eidw	47	3	;	;	:
cord-345088-krb1eidw	47	4	Gallagher	Gallagher	NNP
cord-345088-krb1eidw	47	5	et	et	NNP
cord-345088-krb1eidw	47	6	al	al	NNP
cord-345088-krb1eidw	47	7	.	.	NNP
cord-345088-krb1eidw	47	8	,	,	,
cord-345088-krb1eidw	47	9	1991	1991	CD
cord-345088-krb1eidw	47	10	;	;	:
cord-345088-krb1eidw	47	11	Krueger	Krueger	NNP
cord-345088-krb1eidw	47	12	et	et	NNP
cord-345088-krb1eidw	47	13	al	al	NNP
cord-345088-krb1eidw	47	14	.	.	NNP
cord-345088-krb1eidw	47	15	,	,	,
cord-345088-krb1eidw	47	16	2001	2001	CD
cord-345088-krb1eidw	47	17	;	;	:
cord-345088-krb1eidw	48	1	Luo	Luo	NNP
cord-345088-krb1eidw	48	2	et	et	NNP
cord-345088-krb1eidw	48	3	al	al	NNP
cord-345088-krb1eidw	48	4	.	.	NNP
cord-345088-krb1eidw	48	5	,	,	,
cord-345088-krb1eidw	48	6	1999	1999	CD
cord-345088-krb1eidw	48	7	;	;	:
cord-345088-krb1eidw	48	8	Tsai	Tsai	NNP
cord-345088-krb1eidw	48	9	et	et	NNP
cord-345088-krb1eidw	48	10	al	al	NNP
cord-345088-krb1eidw	48	11	.	.	NNP
cord-345088-krb1eidw	48	12	,	,	,
cord-345088-krb1eidw	48	13	1999	1999	CD
cord-345088-krb1eidw	48	14	)	)	-RRB-
cord-345088-krb1eidw	48	15	.	.	.
cord-345088-krb1eidw	49	1	Cleavage	cleavage	NN
cord-345088-krb1eidw	49	2	of	of	IN
cord-345088-krb1eidw	49	3	the	the	DT
cord-345088-krb1eidw	49	4	S	S	NNP
cord-345088-krb1eidw	49	5	protein	protein	NN
cord-345088-krb1eidw	49	6	enhances	enhance	VBZ
cord-345088-krb1eidw	49	7	the	the	DT
cord-345088-krb1eidw	49	8	membrane	membrane	NN
cord-345088-krb1eidw	49	9	fusion	fusion	NN
cord-345088-krb1eidw	49	10	,	,	,
cord-345088-krb1eidw	49	11	though	though	IN
cord-345088-krb1eidw	49	12	not	not	RB
cord-345088-krb1eidw	49	13	necessarily	necessarily	RB
cord-345088-krb1eidw	49	14	required	require	VBN
cord-345088-krb1eidw	49	15	.	.	.
cord-345088-krb1eidw	50	1	The	the	DT
cord-345088-krb1eidw	50	2	S	S	NNP
cord-345088-krb1eidw	50	3	protein	protein	NN
cord-345088-krb1eidw	50	4	contains	contain	VBZ
cord-345088-krb1eidw	50	5	receptor	receptor	NN
cord-345088-krb1eidw	50	6	-	-	HYPH
cord-345088-krb1eidw	50	7	binding	bind	VBG
cord-345088-krb1eidw	50	8	domains	domain	NNS
cord-345088-krb1eidw	50	9	in	in	IN
cord-345088-krb1eidw	50	10	the	the	DT
cord-345088-krb1eidw	50	11	S1	s1	NN
cord-345088-krb1eidw	50	12	subunit	subunit	NN
cord-345088-krb1eidw	50	13	(	(	-LRB-
cord-345088-krb1eidw	50	14	Kubo	Kubo	NNP
cord-345088-krb1eidw	50	15	et	et	FW
cord-345088-krb1eidw	50	16	al	al	NNP
cord-345088-krb1eidw	50	17	.	.	.
cord-345088-krb1eidw	51	1	,	,	,
cord-345088-krb1eidw	51	2	1994	1994	CD
cord-345088-krb1eidw	51	3	;	;	:
cord-345088-krb1eidw	51	4	Godet	Godet	NNP
cord-345088-krb1eidw	51	5	et	et	NNP
cord-345088-krb1eidw	51	6	al	al	NNP
cord-345088-krb1eidw	51	7	.	.	.
cord-345088-krb1eidw	52	1	,	,	,
cord-345088-krb1eidw	52	2	1994	1994	CD
cord-345088-krb1eidw	52	3	;	;	:
cord-345088-krb1eidw	52	4	Saeki	Saeki	NNP
cord-345088-krb1eidw	52	5	et	et	FW
cord-345088-krb1eidw	52	6	al	al	NNP
cord-345088-krb1eidw	52	7	.	.	NNP
cord-345088-krb1eidw	52	8	,	,	,
cord-345088-krb1eidw	52	9	1997	1997	CD
cord-345088-krb1eidw	52	10	;	;	:
cord-345088-krb1eidw	52	11	Suzuki	Suzuki	NNP
cord-345088-krb1eidw	52	12	and	and	CC
cord-345088-krb1eidw	52	13	Taguchi	Taguchi	NNP
cord-345088-krb1eidw	52	14	,	,	,
cord-345088-krb1eidw	52	15	1996	1996	CD
cord-345088-krb1eidw	52	16	)	)	-RRB-
cord-345088-krb1eidw	52	17	.	.	.
cord-345088-krb1eidw	53	1	Upon	upon	IN
cord-345088-krb1eidw	53	2	binding	bind	VBG
cord-345088-krb1eidw	53	3	of	of	IN
cord-345088-krb1eidw	53	4	the	the	DT
cord-345088-krb1eidw	53	5	S	S	NNP
cord-345088-krb1eidw	53	6	protein	protein	NN
cord-345088-krb1eidw	53	7	to	to	IN
cord-345088-krb1eidw	53	8	the	the	DT
cord-345088-krb1eidw	53	9	receptor(s	receptor(s	NNP
cord-345088-krb1eidw	53	10	)	)	-RRB-
cord-345088-krb1eidw	53	11	,	,	,
cord-345088-krb1eidw	53	12	conformation	conformation	NN
cord-345088-krb1eidw	53	13	changes	change	NNS
cord-345088-krb1eidw	53	14	induce	induce	VBP
cord-345088-krb1eidw	53	15	the	the	DT
cord-345088-krb1eidw	53	16	virus	virus	NN
cord-345088-krb1eidw	53	17	-cell	-cell	CD
cord-345088-krb1eidw	53	18	membrane	membrane	NN
cord-345088-krb1eidw	53	19	fusion	fusion	NN
cord-345088-krb1eidw	53	20	and	and	CC
cord-345088-krb1eidw	53	21	subsequently	subsequently	RB
cord-345088-krb1eidw	53	22	unloading	unload	VBG
cord-345088-krb1eidw	53	23	of	of	IN
cord-345088-krb1eidw	53	24	the	the	DT
cord-345088-krb1eidw	53	25	ribonucleocapsid	ribonucleocapsid	NN
cord-345088-krb1eidw	53	26	inside	inside	IN
cord-345088-krb1eidw	53	27	the	the	DT
cord-345088-krb1eidw	53	28	cell	cell	NN
cord-345088-krb1eidw	53	29	.	.	.
cord-345088-krb1eidw	54	1	It	-PRON-	PRP
cord-345088-krb1eidw	54	2	has	have	VBZ
cord-345088-krb1eidw	54	3	been	be	VBN
cord-345088-krb1eidw	54	4	demonstrated	demonstrate	VBN
cord-345088-krb1eidw	54	5	that	that	IN
cord-345088-krb1eidw	54	6	the	the	DT
cord-345088-krb1eidw	54	7	cell	cell	NN
cord-345088-krb1eidw	54	8	-	-	HYPH
cord-345088-krb1eidw	54	9	to	to	IN
cord-345088-krb1eidw	54	10	-	-	HYPH
cord-345088-krb1eidw	54	11	cell	cell	NN
cord-345088-krb1eidw	54	12	spread	spread	NN
cord-345088-krb1eidw	54	13	of	of	IN
cord-345088-krb1eidw	54	14	MHV	MHV	NNP
cord-345088-krb1eidw	54	15	infection	infection	NN
cord-345088-krb1eidw	54	16	does	do	VBZ
cord-345088-krb1eidw	54	17	not	not	RB
cord-345088-krb1eidw	54	18	need	need	VB
cord-345088-krb1eidw	54	19	the	the	DT
cord-345088-krb1eidw	54	20	presence	presence	NN
cord-345088-krb1eidw	54	21	of	of	IN
cord-345088-krb1eidw	54	22	the	the	DT
cord-345088-krb1eidw	54	23	receptor	receptor	NN
cord-345088-krb1eidw	54	24	,	,	,
cord-345088-krb1eidw	54	25	but	but	CC
cord-345088-krb1eidw	54	26	the	the	DT
cord-345088-krb1eidw	54	27	S	S	NNP
cord-345088-krb1eidw	54	28	protein	protein	NN
cord-345088-krb1eidw	54	29	is	be	VBZ
cord-345088-krb1eidw	54	30	required	require	VBN
cord-345088-krb1eidw	54	31	to	to	TO
cord-345088-krb1eidw	54	32	mediate	mediate	VB
cord-345088-krb1eidw	54	33	the	the	DT
cord-345088-krb1eidw	54	34	cell	cell	NN
cord-345088-krb1eidw	54	35	-	-	HYPH
cord-345088-krb1eidw	54	36	to	to	IN
cord-345088-krb1eidw	54	37	-	-	HYPH
cord-345088-krb1eidw	54	38	cell	cell	NN
cord-345088-krb1eidw	54	39	fusion	fusion	NN
cord-345088-krb1eidw	54	40	(	(	-LRB-
cord-345088-krb1eidw	54	41	Gallagher	Gallagher	NNP
cord-345088-krb1eidw	54	42	et	et	NNP
cord-345088-krb1eidw	54	43	al	al	NNP
cord-345088-krb1eidw	54	44	.	.	NNP
cord-345088-krb1eidw	54	45	,	,	,
cord-345088-krb1eidw	54	46	1992	1992	CD
cord-345088-krb1eidw	54	47	;	;	:
cord-345088-krb1eidw	54	48	Nash	Nash	NNP
cord-345088-krb1eidw	54	49	and	and	CC
cord-345088-krb1eidw	54	50	Buchmeier	Buchmeier	NNP
cord-345088-krb1eidw	54	51	,	,	,
cord-345088-krb1eidw	54	52	1996	1996	CD
cord-345088-krb1eidw	54	53	)	)	-RRB-
cord-345088-krb1eidw	54	54	.	.	.
cord-345088-krb1eidw	55	1	For	for	IN
cord-345088-krb1eidw	55	2	coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	55	3	,	,	,
cord-345088-krb1eidw	55	4	however	however	RB
cord-345088-krb1eidw	55	5	,	,	,
cord-345088-krb1eidw	55	6	the	the	DT
cord-345088-krb1eidw	55	7	detail	detail	NN
cord-345088-krb1eidw	55	8	of	of	IN
cord-345088-krb1eidw	55	9	molecular	molecular	JJ
cord-345088-krb1eidw	55	10	mechanisms	mechanism	NNS
cord-345088-krb1eidw	55	11	of	of	IN
cord-345088-krb1eidw	55	12	membrane	membrane	NN
cord-345088-krb1eidw	55	13	fusion	fusion	NN
cord-345088-krb1eidw	55	14	,	,	,
cord-345088-krb1eidw	55	15	especially	especially	RB
cord-345088-krb1eidw	55	16	those	those	DT
cord-345088-krb1eidw	55	17	linked	link	VBN
cord-345088-krb1eidw	55	18	to	to	IN
cord-345088-krb1eidw	55	19	the	the	DT
cord-345088-krb1eidw	55	20	S1	S1	NNP
cord-345088-krb1eidw	55	21	region	region	NN
cord-345088-krb1eidw	55	22	,	,	,
cord-345088-krb1eidw	55	23	is	be	VBZ
cord-345088-krb1eidw	55	24	yet	yet	RB
cord-345088-krb1eidw	55	25	to	to	TO
cord-345088-krb1eidw	55	26	be	be	VB
cord-345088-krb1eidw	55	27	fully	fully	RB
cord-345088-krb1eidw	55	28	elucidated	elucidate	VBN
cord-345088-krb1eidw	55	29	.	.	.
cord-345088-krb1eidw	56	1	In	in	IN
cord-345088-krb1eidw	56	2	this	this	DT
cord-345088-krb1eidw	56	3	study	study	NN
cord-345088-krb1eidw	56	4	,	,	,
cord-345088-krb1eidw	56	5	ts	ts	JJ
cord-345088-krb1eidw	56	6	mutants	mutant	NNS
cord-345088-krb1eidw	56	7	were	be	VBD
cord-345088-krb1eidw	56	8	generated	generate	VBN
cord-345088-krb1eidw	56	9	by	by	IN
cord-345088-krb1eidw	56	10	growing	grow	VBG
cord-345088-krb1eidw	56	11	the	the	DT
cord-345088-krb1eidw	56	12	Beaudette	Beaudette	NNP
cord-345088-krb1eidw	56	13	strain	strain	NN
cord-345088-krb1eidw	56	14	of	of	IN
cord-345088-krb1eidw	56	15	IBV	IBV	NNP
cord-345088-krb1eidw	56	16	at	at	IN
cord-345088-krb1eidw	56	17	progressively	progressively	RB
cord-345088-krb1eidw	56	18	lower	low	JJR
cord-345088-krb1eidw	56	19	temperatures	temperature	NNS
cord-345088-krb1eidw	56	20	from	from	IN
cord-345088-krb1eidw	56	21	35	35	CD
cord-345088-krb1eidw	56	22	to	to	TO
cord-345088-krb1eidw	56	23	28	28	CD
cord-345088-krb1eidw	56	24	jC	jC	NNP
cord-345088-krb1eidw	56	25	in	in	IN
cord-345088-krb1eidw	56	26	Vero	Vero	NNP
cord-345088-krb1eidw	56	27	cells	cell	NNS
cord-345088-krb1eidw	56	28	.	.	.
cord-345088-krb1eidw	57	1	Two	two	CD
cord-345088-krb1eidw	57	2	ts	ts	NN
cord-345088-krb1eidw	57	3	mutants	mutant	NNS
cord-345088-krb1eidw	57	4	were	be	VBD
cord-345088-krb1eidw	57	5	isolated	isolate	VBN
cord-345088-krb1eidw	57	6	from	from	IN
cord-345088-krb1eidw	57	7	passages	passage	NNS
cord-345088-krb1eidw	57	8	grown	grow	VBN
cord-345088-krb1eidw	57	9	at	at	IN
cord-345088-krb1eidw	57	10	29	29	CD
cord-345088-krb1eidw	57	11	jC	jC	NNP
cord-345088-krb1eidw	57	12	(	(	-LRB-
cord-345088-krb1eidw	57	13	ts291602	ts291602	NNP
cord-345088-krb1eidw	57	14	)	)	-RRB-
cord-345088-krb1eidw	57	15	and	and	CC
cord-345088-krb1eidw	57	16	28	28	CD
cord-345088-krb1eidw	57	17	jC	jC	NNP
cord-345088-krb1eidw	57	18	(	(	-LRB-
cord-345088-krb1eidw	57	19	ts282902	ts282902	NN
cord-345088-krb1eidw	57	20	)	)	-RRB-
cord-345088-krb1eidw	57	21	.	.	.
cord-345088-krb1eidw	58	1	A	a	DT
cord-345088-krb1eidw	58	2	revertant	revertant	NN
cord-345088-krb1eidw	58	3	was	be	VBD
cord-345088-krb1eidw	58	4	also	also	RB
cord-345088-krb1eidw	58	5	obtained	obtain	VBN
cord-345088-krb1eidw	58	6	by	by	IN
cord-345088-krb1eidw	58	7	growing	grow	VBG
cord-345088-krb1eidw	58	8	the	the	DT
cord-345088-krb1eidw	58	9	ts	ts	NNP
cord-345088-krb1eidw	58	10	mutant	mutant	NN
cord-345088-krb1eidw	58	11	at	at	IN
cord-345088-krb1eidw	58	12	the	the	DT
cord-345088-krb1eidw	58	13	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	58	14	temperature	temperature	NN
cord-345088-krb1eidw	58	15	(	(	-LRB-
cord-345088-krb1eidw	58	16	40	40	CD
cord-345088-krb1eidw	58	17	jC	jC	NNP
cord-345088-krb1eidw	58	18	)	)	-RRB-
cord-345088-krb1eidw	58	19	.	.	.
cord-345088-krb1eidw	59	1	Sequence	sequence	NN
cord-345088-krb1eidw	59	2	comparison	comparison	NN
cord-345088-krb1eidw	59	3	revealed	reveal	VBD
cord-345088-krb1eidw	59	4	that	that	IN
cord-345088-krb1eidw	59	5	mutations	mutation	NNS
cord-345088-krb1eidw	59	6	in	in	IN
cord-345088-krb1eidw	59	7	the	the	DT
cord-345088-krb1eidw	59	8	S1	S1	NNP
cord-345088-krb1eidw	59	9	subunit	subunit	NN
cord-345088-krb1eidw	59	10	were	be	VBD
cord-345088-krb1eidw	59	11	responsible	responsible	JJ
cord-345088-krb1eidw	59	12	for	for	IN
cord-345088-krb1eidw	59	13	the	the	DT
cord-345088-krb1eidw	59	14	ts	ts	NNP
cord-345088-krb1eidw	59	15	phenotype	phenotype	NN
cord-345088-krb1eidw	59	16	.	.	.
cord-345088-krb1eidw	60	1	Comparative	comparative	JJ
cord-345088-krb1eidw	60	2	studies	study	NNS
cord-345088-krb1eidw	60	3	of	of	IN
cord-345088-krb1eidw	60	4	viral	viral	JJ
cord-345088-krb1eidw	60	5	protein	protein	NN
cord-345088-krb1eidw	60	6	synthesis	synthesis	NN
cord-345088-krb1eidw	60	7	and	and	CC
cord-345088-krb1eidw	60	8	growth	growth	NN
cord-345088-krb1eidw	60	9	properties	property	NNS
cord-345088-krb1eidw	60	10	of	of	IN
cord-345088-krb1eidw	60	11	the	the	DT
cord-345088-krb1eidw	60	12	wt	wt	NN
cord-345088-krb1eidw	60	13	,	,	,
cord-345088-krb1eidw	60	14	ts	ts	NNP
cord-345088-krb1eidw	60	15	mutant	mutant	NN
cord-345088-krb1eidw	60	16	,	,	,
cord-345088-krb1eidw	60	17	and	and	CC
cord-345088-krb1eidw	60	18	revertant	revertant	NN
cord-345088-krb1eidw	60	19	viruses	virus	NNS
cord-345088-krb1eidw	60	20	demonstrated	demonstrate	VBD
cord-345088-krb1eidw	60	21	that	that	IN
cord-345088-krb1eidw	60	22	the	the	DT
cord-345088-krb1eidw	60	23	ts	ts	JJ
cord-345088-krb1eidw	60	24	mutants	mutant	NNS
cord-345088-krb1eidw	60	25	were	be	VBD
cord-345088-krb1eidw	60	26	extremely	extremely	RB
cord-345088-krb1eidw	60	27	unstable	unstable	JJ
cord-345088-krb1eidw	60	28	at	at	IN
cord-345088-krb1eidw	60	29	the	the	DT
cord-345088-krb1eidw	60	30	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	60	31	temperature	temperature	NN
cord-345088-krb1eidw	60	32	.	.	.
cord-345088-krb1eidw	61	1	If	if	IN
cord-345088-krb1eidw	61	2	they	-PRON-	PRP
cord-345088-krb1eidw	61	3	were	be	VBD
cord-345088-krb1eidw	61	4	allowed	allow	VBN
cord-345088-krb1eidw	61	5	to	to	TO
cord-345088-krb1eidw	61	6	enter	enter	VB
cord-345088-krb1eidw	61	7	cells	cell	NNS
cord-345088-krb1eidw	61	8	by	by	IN
cord-345088-krb1eidw	61	9	absorption	absorption	NN
cord-345088-krb1eidw	61	10	for	for	IN
cord-345088-krb1eidw	61	11	1	1	CD
cord-345088-krb1eidw	61	12	h	h	NN
cord-345088-krb1eidw	61	13	at	at	IN
cord-345088-krb1eidw	61	14	the	the	DT
cord-345088-krb1eidw	61	15	permissive	permissive	JJ
cord-345088-krb1eidw	61	16	temperature	temperature	NN
cord-345088-krb1eidw	61	17	(	(	-LRB-
cord-345088-krb1eidw	61	18	32	32	CD
cord-345088-krb1eidw	61	19	jC	jC	NNP
cord-345088-krb1eidw	61	20	)	)	-RRB-
cord-345088-krb1eidw	61	21	,	,	,
cord-345088-krb1eidw	61	22	the	the	DT
cord-345088-krb1eidw	61	23	S	S	NNP
cord-345088-krb1eidw	61	24	protein	protein	NN
cord-345088-krb1eidw	61	25	would	would	MD
cord-345088-krb1eidw	61	26	be	be	VB
cord-345088-krb1eidw	61	27	core	core	NN
cord-345088-krb1eidw	61	28	-	-	HYPH
cord-345088-krb1eidw	61	29	glycosylated	glycosylated	JJ
cord-345088-krb1eidw	61	30	and	and	CC
cord-345088-krb1eidw	61	31	partially	partially	RB
cord-345088-krb1eidw	61	32	modified	modify	VBN
cord-345088-krb1eidw	61	33	at	at	IN
cord-345088-krb1eidw	61	34	the	the	DT
cord-345088-krb1eidw	61	35	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	61	36	temperature	temperature	NN
cord-345088-krb1eidw	61	37	,	,	,
cord-345088-krb1eidw	61	38	but	but	CC
cord-345088-krb1eidw	61	39	was	be	VBD
cord-345088-krb1eidw	61	40	unable	unable	JJ
cord-345088-krb1eidw	61	41	to	to	TO
cord-345088-krb1eidw	61	42	assemble	assemble	VB
cord-345088-krb1eidw	61	43	into	into	IN
cord-345088-krb1eidw	61	44	virions	virion	NNS
cord-345088-krb1eidw	61	45	.	.	.
cord-345088-krb1eidw	62	1	The	the	DT
cord-345088-krb1eidw	62	2	membrane	membrane	NN
cord-345088-krb1eidw	62	3	fusion	fusion	NN
cord-345088-krb1eidw	62	4	activities	activity	NNS
cord-345088-krb1eidw	62	5	of	of	IN
cord-345088-krb1eidw	62	6	the	the	DT
cord-345088-krb1eidw	62	7	ts	ts	NNP
cord-345088-krb1eidw	62	8	mutants	mutant	NNS
cord-345088-krb1eidw	62	9	were	be	VBD
cord-345088-krb1eidw	62	10	totally	totally	RB
cord-345088-krb1eidw	62	11	abolished	abolish	VBN
cord-345088-krb1eidw	62	12	in	in	IN
cord-345088-krb1eidw	62	13	virus	virus	NN
cord-345088-krb1eidw	62	14	-	-	HYPH
cord-345088-krb1eidw	62	15	infected	infect	VBN
cord-345088-krb1eidw	62	16	cells	cell	NNS
cord-345088-krb1eidw	62	17	and	and	CC
cord-345088-krb1eidw	62	18	in	in	IN
cord-345088-krb1eidw	62	19	cells	cell	NNS
cord-345088-krb1eidw	62	20	overexpressing	overexpresse	VBG
cord-345088-krb1eidw	62	21	the	the	DT
cord-345088-krb1eidw	62	22	mutant	mutant	JJ
cord-345088-krb1eidw	62	23	S	S	NNP
cord-345088-krb1eidw	62	24	protein	protein	NN
cord-345088-krb1eidw	62	25	at	at	IN
cord-345088-krb1eidw	62	26	the	the	DT
cord-345088-krb1eidw	62	27	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	62	28	temperature	temperature	NN
cord-345088-krb1eidw	62	29	.	.	.
cord-345088-krb1eidw	63	1	The	the	DT
cord-345088-krb1eidw	63	2	Beaudette	Beaudette	NNP
cord-345088-krb1eidw	63	3	strain	strain	NN
cord-345088-krb1eidw	63	4	of	of	IN
cord-345088-krb1eidw	63	5	IBV	IBV	NNP
cord-345088-krb1eidw	63	6	,	,	,
cord-345088-krb1eidw	63	7	grown	grow	VBN
cord-345088-krb1eidw	63	8	in	in	IN
cord-345088-krb1eidw	63	9	Vero	Vero	NNP
cord-345088-krb1eidw	63	10	cells	cell	NNS
cord-345088-krb1eidw	63	11	at	at	IN
cord-345088-krb1eidw	63	12	37	37	CD
cord-345088-krb1eidw	63	13	jC	jC	NNS
cord-345088-krb1eidw	63	14	,	,	,
cord-345088-krb1eidw	63	15	was	be	VBD
cord-345088-krb1eidw	63	16	plaque	plaque	NN
cord-345088-krb1eidw	63	17	-	-	HYPH
cord-345088-krb1eidw	63	18	purified	purify	VBN
cord-345088-krb1eidw	63	19	and	and	CC
cord-345088-krb1eidw	63	20	was	be	VBD
cord-345088-krb1eidw	63	21	initially	initially	RB
cord-345088-krb1eidw	63	22	adapted	adapt	VBN
cord-345088-krb1eidw	63	23	to	to	TO
cord-345088-krb1eidw	63	24	grow	grow	VB
cord-345088-krb1eidw	63	25	at	at	IN
cord-345088-krb1eidw	63	26	35	35	CD
cord-345088-krb1eidw	63	27	jC.	jc.	NN
cord-345088-krb1eidw	64	1	After	after	IN
cord-345088-krb1eidw	64	2	4	4	CD
cord-345088-krb1eidw	64	3	passages	passage	NNS
cord-345088-krb1eidw	64	4	,	,	,
cord-345088-krb1eidw	64	5	the	the	DT
cord-345088-krb1eidw	64	6	virus	virus	NN
cord-345088-krb1eidw	64	7	was	be	VBD
cord-345088-krb1eidw	64	8	subsequently	subsequently	RB
cord-345088-krb1eidw	64	9	adapted	adapt	VBN
cord-345088-krb1eidw	64	10	to	to	TO
cord-345088-krb1eidw	64	11	grow	grow	VB
cord-345088-krb1eidw	64	12	at	at	IN
cord-345088-krb1eidw	64	13	progressively	progressively	RB
cord-345088-krb1eidw	64	14	lower	low	JJR
cord-345088-krb1eidw	64	15	temperatures	temperature	NNS
cord-345088-krb1eidw	64	16	by	by	IN
cord-345088-krb1eidw	64	17	culturing	culture	VBG
cord-345088-krb1eidw	64	18	at	at	IN
cord-345088-krb1eidw	64	19	34	34	CD
cord-345088-krb1eidw	64	20	jC	jC	NNS
cord-345088-krb1eidw	64	21	for	for	IN
cord-345088-krb1eidw	64	22	5	5	CD
cord-345088-krb1eidw	64	23	passages	passage	NNS
cord-345088-krb1eidw	64	24	,	,	,
cord-345088-krb1eidw	64	25	33	33	CD
cord-345088-krb1eidw	64	26	jC	jc	NN
cord-345088-krb1eidw	64	27	for	for	IN
cord-345088-krb1eidw	64	28	7	7	CD
cord-345088-krb1eidw	64	29	passages	passage	NNS
cord-345088-krb1eidw	64	30	,	,	,
cord-345088-krb1eidw	64	31	32	32	CD
cord-345088-krb1eidw	64	32	jC	jC	NNS
cord-345088-krb1eidw	64	33	for	for	IN
cord-345088-krb1eidw	64	34	44	44	CD
cord-345088-krb1eidw	64	35	passages	passage	NNS
cord-345088-krb1eidw	64	36	,	,	,
cord-345088-krb1eidw	64	37	30	30	CD
cord-345088-krb1eidw	64	38	jC	jc	NN
cord-345088-krb1eidw	64	39	for	for	IN
cord-345088-krb1eidw	64	40	12	12	CD
cord-345088-krb1eidw	64	41	passages	passage	NNS
cord-345088-krb1eidw	64	42	,	,	,
cord-345088-krb1eidw	64	43	29	29	CD
cord-345088-krb1eidw	64	44	jC	jC	NNS
cord-345088-krb1eidw	64	45	for	for	IN
cord-345088-krb1eidw	64	46	21	21	CD
cord-345088-krb1eidw	64	47	passages	passage	NNS
cord-345088-krb1eidw	64	48	,	,	,
cord-345088-krb1eidw	64	49	and	and	CC
cord-345088-krb1eidw	64	50	28	28	CD
cord-345088-krb1eidw	64	51	jC	jC	NNS
cord-345088-krb1eidw	64	52	for	for	IN
cord-345088-krb1eidw	64	53	29	29	CD
cord-345088-krb1eidw	64	54	passages	passage	NNS
cord-345088-krb1eidw	64	55	.	.	.
cord-345088-krb1eidw	65	1	When	when	WRB
cord-345088-krb1eidw	65	2	the	the	DT
cord-345088-krb1eidw	65	3	virus	virus	NN
cord-345088-krb1eidw	65	4	was	be	VBD
cord-345088-krb1eidw	65	5	initially	initially	RB
cord-345088-krb1eidw	65	6	adapted	adapt	VBN
cord-345088-krb1eidw	65	7	to	to	IN
cord-345088-krb1eidw	65	8	a	a	DT
cord-345088-krb1eidw	65	9	lower	low	JJR
cord-345088-krb1eidw	65	10	temperature	temperature	NN
cord-345088-krb1eidw	65	11	,	,	,
cord-345088-krb1eidw	65	12	no	no	DT
cord-345088-krb1eidw	65	13	CPE	CPE	NNP
cord-345088-krb1eidw	65	14	was	be	VBD
cord-345088-krb1eidw	65	15	observed	observe	VBN
cord-345088-krb1eidw	65	16	within	within	IN
cord-345088-krb1eidw	65	17	72	72	CD
cord-345088-krb1eidw	65	18	-96	-96	SYM
cord-345088-krb1eidw	65	19	h	h	NN
cord-345088-krb1eidw	65	20	postinfection	postinfection	NN
cord-345088-krb1eidw	65	21	.	.	.
cord-345088-krb1eidw	66	1	Apparent	apparent	JJ
cord-345088-krb1eidw	66	2	CPE	CPE	NNP
cord-345088-krb1eidw	66	3	appeared	appear	VBD
cord-345088-krb1eidw	66	4	usually	usually	RB
cord-345088-krb1eidw	66	5	after	after	IN
cord-345088-krb1eidw	66	6	2	2	CD
cord-345088-krb1eidw	66	7	to	to	TO
cord-345088-krb1eidw	66	8	5	5	CD
cord-345088-krb1eidw	66	9	passages	passage	NNS
cord-345088-krb1eidw	66	10	.	.	.
cord-345088-krb1eidw	67	1	The	the	DT
cord-345088-krb1eidw	67	2	virus	virus	NN
cord-345088-krb1eidw	67	3	was	be	VBD
cord-345088-krb1eidw	67	4	continuously	continuously	RB
cord-345088-krb1eidw	67	5	passaged	passage	VBN
cord-345088-krb1eidw	67	6	at	at	IN
cord-345088-krb1eidw	67	7	the	the	DT
cord-345088-krb1eidw	67	8	same	same	JJ
cord-345088-krb1eidw	67	9	temperature	temperature	NN
cord-345088-krb1eidw	67	10	until	until	IN
cord-345088-krb1eidw	67	11	CPE	CPE	NNP
cord-345088-krb1eidw	67	12	became	become	VBD
cord-345088-krb1eidw	67	13	visible	visible	JJ
cord-345088-krb1eidw	67	14	within	within	IN
cord-345088-krb1eidw	67	15	24	24	CD
cord-345088-krb1eidw	67	16	-48	-48	NNP
cord-345088-krb1eidw	67	17	h	h	NNP
cord-345088-krb1eidw	67	18	postinfection	postinfection	NN
cord-345088-krb1eidw	67	19	,	,	,
cord-345088-krb1eidw	67	20	and	and	CC
cord-345088-krb1eidw	67	21	then	then	RB
cord-345088-krb1eidw	67	22	shifted	shift	VBD
cord-345088-krb1eidw	67	23	to	to	IN
cord-345088-krb1eidw	67	24	a	a	DT
cord-345088-krb1eidw	67	25	lower	low	JJR
cord-345088-krb1eidw	67	26	temperature	temperature	NN
cord-345088-krb1eidw	67	27	.	.	.
cord-345088-krb1eidw	68	1	Plaque	plaque	NN
cord-345088-krb1eidw	68	2	assays	assay	NNS
cord-345088-krb1eidw	68	3	of	of	IN
cord-345088-krb1eidw	68	4	different	different	JJ
cord-345088-krb1eidw	68	5	passages	passage	NNS
cord-345088-krb1eidw	68	6	were	be	VBD
cord-345088-krb1eidw	68	7	performed	perform	VBN
cord-345088-krb1eidw	68	8	at	at	IN
cord-345088-krb1eidw	68	9	both	both	DT
cord-345088-krb1eidw	68	10	32	32	CD
cord-345088-krb1eidw	68	11	and	and	CC
cord-345088-krb1eidw	68	12	40	40	CD
cord-345088-krb1eidw	68	13	jC.	jc.	NN
cord-345088-krb1eidw	69	1	It	-PRON-	PRP
cord-345088-krb1eidw	69	2	was	be	VBD
cord-345088-krb1eidw	69	3	found	find	VBN
cord-345088-krb1eidw	69	4	that	that	IN
cord-345088-krb1eidw	69	5	viruses	virus	NNS
cord-345088-krb1eidw	69	6	from	from	IN
cord-345088-krb1eidw	69	7	early	early	JJ
cord-345088-krb1eidw	69	8	passages	passage	NNS
cord-345088-krb1eidw	69	9	grown	grow	VBN
cord-345088-krb1eidw	69	10	at	at	IN
cord-345088-krb1eidw	69	11	32	32	CD
cord-345088-krb1eidw	69	12	jC	jC	NNP
cord-345088-krb1eidw	69	13	could	could	MD
cord-345088-krb1eidw	69	14	form	form	VB
cord-345088-krb1eidw	69	15	plaques	plaque	NNS
cord-345088-krb1eidw	69	16	at	at	IN
cord-345088-krb1eidw	69	17	both	both	DT
cord-345088-krb1eidw	69	18	temperatures	temperature	NNS
cord-345088-krb1eidw	69	19	at	at	IN
cord-345088-krb1eidw	69	20	similar	similar	JJ
cord-345088-krb1eidw	69	21	levels	level	NNS
cord-345088-krb1eidw	69	22	.	.	.
cord-345088-krb1eidw	70	1	But	but	CC
cord-345088-krb1eidw	70	2	passages	passage	NNS
cord-345088-krb1eidw	70	3	grown	grow	VBN
cord-345088-krb1eidw	70	4	at	at	IN
cord-345088-krb1eidw	70	5	29	29	CD
cord-345088-krb1eidw	71	1	jC	jC	NNP
cord-345088-krb1eidw	71	2	produced	produce	VBD
cord-345088-krb1eidw	71	3	much	much	RB
cord-345088-krb1eidw	71	4	fewer	few	JJR
cord-345088-krb1eidw	71	5	plaques	plaque	NNS
cord-345088-krb1eidw	71	6	at	at	IN
cord-345088-krb1eidw	71	7	40	40	CD
cord-345088-krb1eidw	71	8	jC	jC	NNS
cord-345088-krb1eidw	71	9	than	than	IN
cord-345088-krb1eidw	71	10	at	at	IN
cord-345088-krb1eidw	71	11	32	32	CD
cord-345088-krb1eidw	71	12	jC	jC	NNP
cord-345088-krb1eidw	71	13	,	,	,
cord-345088-krb1eidw	71	14	suggesting	suggest	VBG
cord-345088-krb1eidw	71	15	the	the	DT
cord-345088-krb1eidw	71	16	emergence	emergence	NN
cord-345088-krb1eidw	71	17	of	of	IN
cord-345088-krb1eidw	71	18	ts	ts	JJ
cord-345088-krb1eidw	71	19	mutants	mutant	NNS
cord-345088-krb1eidw	71	20	during	during	IN
cord-345088-krb1eidw	71	21	cold	cold	JJ
cord-345088-krb1eidw	71	22	-	-	HYPH
cord-345088-krb1eidw	71	23	adaptation	adaptation	NN
cord-345088-krb1eidw	71	24	.	.	.
cord-345088-krb1eidw	72	1	These	these	DT
cord-345088-krb1eidw	72	2	ts	ts	JJ
cord-345088-krb1eidw	72	3	mutants	mutant	NNS
cord-345088-krb1eidw	72	4	dominated	dominate	VBN
cord-345088-krb1eidw	72	5	quickly	quickly	RB
cord-345088-krb1eidw	72	6	at	at	IN
cord-345088-krb1eidw	72	7	28	28	CD
cord-345088-krb1eidw	72	8	jC.	jc.	NN
cord-345088-krb1eidw	72	9	Two	two	CD
cord-345088-krb1eidw	72	10	ts	ts	JJ
cord-345088-krb1eidw	72	11	mutants	mutant	NNS
cord-345088-krb1eidw	72	12	,	,	,
cord-345088-krb1eidw	72	13	designated	designate	VBN
cord-345088-krb1eidw	72	14	ts291602	ts291602	NN
cord-345088-krb1eidw	72	15	and	and	CC
cord-345088-krb1eidw	72	16	ts282902	ts282902	XX
cord-345088-krb1eidw	72	17	,	,	,
cord-345088-krb1eidw	72	18	were	be	VBD
cord-345088-krb1eidw	72	19	plaque	plaque	NN
cord-345088-krb1eidw	72	20	-	-	HYPH
cord-345088-krb1eidw	72	21	purified	purify	VBN
cord-345088-krb1eidw	72	22	from	from	IN
cord-345088-krb1eidw	72	23	passage	passage	NN
cord-345088-krb1eidw	72	24	16	16	CD
cord-345088-krb1eidw	72	25	grown	grow	VBN
cord-345088-krb1eidw	72	26	at	at	IN
cord-345088-krb1eidw	72	27	29	29	CD
cord-345088-krb1eidw	72	28	jC	jC	NNP
cord-345088-krb1eidw	72	29	and	and	CC
cord-345088-krb1eidw	72	30	passage	passage	NN
cord-345088-krb1eidw	72	31	29	29	CD
cord-345088-krb1eidw	72	32	grown	grow	VBN
cord-345088-krb1eidw	72	33	at	at	IN
cord-345088-krb1eidw	72	34	28	28	CD
cord-345088-krb1eidw	72	35	jC	jC	NNP
cord-345088-krb1eidw	72	36	,	,	,
cord-345088-krb1eidw	72	37	respectively	respectively	RB
cord-345088-krb1eidw	72	38	.	.	.
cord-345088-krb1eidw	73	1	Plaque	plaque	NN
cord-345088-krb1eidw	73	2	assays	assay	NNS
cord-345088-krb1eidw	73	3	showed	show	VBD
cord-345088-krb1eidw	73	4	that	that	IN
cord-345088-krb1eidw	73	5	both	both	CC
cord-345088-krb1eidw	73	6	ts291602	ts291602	NN
cord-345088-krb1eidw	73	7	and	and	CC
cord-345088-krb1eidw	73	8	ts282902	ts282902	FW
cord-345088-krb1eidw	73	9	formed	form	VBN
cord-345088-krb1eidw	73	10	plaques	plaque	NNS
cord-345088-krb1eidw	73	11	only	only	RB
cord-345088-krb1eidw	73	12	at	at	IN
cord-345088-krb1eidw	73	13	the	the	DT
cord-345088-krb1eidw	73	14	permissive	permissive	JJ
cord-345088-krb1eidw	73	15	temperature	temperature	NN
cord-345088-krb1eidw	73	16	,	,	,
cord-345088-krb1eidw	73	17	but	but	CC
cord-345088-krb1eidw	73	18	not	not	RB
cord-345088-krb1eidw	73	19	at	at	RB
cord-345088-krb1eidw	73	20	all	all	RB
cord-345088-krb1eidw	73	21	at	at	IN
cord-345088-krb1eidw	73	22	the	the	DT
cord-345088-krb1eidw	73	23	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	73	24	temperature	temperature	NN
cord-345088-krb1eidw	73	25	.	.	.
cord-345088-krb1eidw	74	1	By	by	IN
cord-345088-krb1eidw	74	2	growing	grow	VBG
cord-345088-krb1eidw	74	3	the	the	DT
cord-345088-krb1eidw	74	4	ts	ts	NNP
cord-345088-krb1eidw	74	5	mutant	mutant	NN
cord-345088-krb1eidw	74	6	at	at	IN
cord-345088-krb1eidw	74	7	37	37	CD
cord-345088-krb1eidw	74	8	jC	jC	NNS
cord-345088-krb1eidw	74	9	overnight	overnight	RB
cord-345088-krb1eidw	74	10	and	and	CC
cord-345088-krb1eidw	74	11	then	then	RB
cord-345088-krb1eidw	74	12	at	at	IN
cord-345088-krb1eidw	74	13	40	40	CD
cord-345088-krb1eidw	74	14	jC	jC	NNS
cord-345088-krb1eidw	74	15	for	for	IN
cord-345088-krb1eidw	74	16	2	2	CD
cord-345088-krb1eidw	74	17	days	day	NNS
cord-345088-krb1eidw	74	18	,	,	,
cord-345088-krb1eidw	74	19	a	a	DT
cord-345088-krb1eidw	74	20	revertant	revertant	NN
cord-345088-krb1eidw	74	21	,	,	,
cord-345088-krb1eidw	75	1	rev-1	rev-1	NNP
cord-345088-krb1eidw	75	2	,	,	,
cord-345088-krb1eidw	75	3	was	be	VBD
cord-345088-krb1eidw	75	4	obtained	obtain	VBN
cord-345088-krb1eidw	75	5	which	which	WDT
cord-345088-krb1eidw	75	6	produced	produce	VBD
cord-345088-krb1eidw	75	7	plaques	plaque	NNS
cord-345088-krb1eidw	75	8	at	at	IN
cord-345088-krb1eidw	75	9	both	both	DT
cord-345088-krb1eidw	75	10	32	32	CD
cord-345088-krb1eidw	75	11	and	and	CC
cord-345088-krb1eidw	75	12	40	40	CD
cord-345088-krb1eidw	75	13	jC	jC	NNS
cord-345088-krb1eidw	75	14	at	at	IN
cord-345088-krb1eidw	75	15	a	a	DT
cord-345088-krb1eidw	75	16	similar	similar	JJ
cord-345088-krb1eidw	75	17	level	level	NN
cord-345088-krb1eidw	75	18	(	(	-LRB-
cord-345088-krb1eidw	75	19	Table	table	NN
cord-345088-krb1eidw	75	20	1	1	CD
cord-345088-krb1eidw	75	21	)	)	-RRB-
cord-345088-krb1eidw	75	22	.	.	.
cord-345088-krb1eidw	76	1	The	the	DT
cord-345088-krb1eidw	76	2	titers	titer	NNS
cord-345088-krb1eidw	76	3	of	of	IN
cord-345088-krb1eidw	76	4	rev-1	rev-1	NNP
cord-345088-krb1eidw	76	5	were	be	VBD
cord-345088-krb1eidw	76	6	much	much	RB
cord-345088-krb1eidw	76	7	higher	high	JJR
cord-345088-krb1eidw	76	8	than	than	IN
cord-345088-krb1eidw	76	9	that	that	DT
cord-345088-krb1eidw	76	10	of	of	IN
cord-345088-krb1eidw	76	11	the	the	DT
cord-345088-krb1eidw	76	12	wild	wild	JJ
cord-345088-krb1eidw	76	13	-	-	HYPH
cord-345088-krb1eidw	76	14	type	type	NN
cord-345088-krb1eidw	76	15	virus	virus	NN
cord-345088-krb1eidw	76	16	wt6501	wt6501	NN
cord-345088-krb1eidw	76	17	(	(	-LRB-
cord-345088-krb1eidw	76	18	isolated	isolate	VBN
cord-345088-krb1eidw	76	19	from	from	IN
cord-345088-krb1eidw	76	20	passage	passage	NN
cord-345088-krb1eidw	76	21	65	65	CD
cord-345088-krb1eidw	76	22	at	at	IN
cord-345088-krb1eidw	76	23	37	37	CD
cord-345088-krb1eidw	76	24	jC	jC	NNP
cord-345088-krb1eidw	76	25	)	)	-RRB-
cord-345088-krb1eidw	76	26	at	at	IN
cord-345088-krb1eidw	76	27	32	32	CD
cord-345088-krb1eidw	76	28	jC	jC	NNP
cord-345088-krb1eidw	76	29	,	,	,
cord-345088-krb1eidw	76	30	as	as	IN
cord-345088-krb1eidw	76	31	it	-PRON-	PRP
cord-345088-krb1eidw	76	32	was	be	VBD
cord-345088-krb1eidw	76	33	derived	derive	VBN
cord-345088-krb1eidw	76	34	from	from	IN
cord-345088-krb1eidw	76	35	the	the	DT
cord-345088-krb1eidw	76	36	cold	cold	NN
cord-345088-krb1eidw	76	37	-	-	HYPH
cord-345088-krb1eidw	76	38	adapted	adapt	VBN
cord-345088-krb1eidw	76	39	virus	virus	NN
cord-345088-krb1eidw	76	40	(	(	-LRB-
cord-345088-krb1eidw	76	41	Table	table	NN
cord-345088-krb1eidw	76	42	1	1	CD
cord-345088-krb1eidw	76	43	)	)	-RRB-
cord-345088-krb1eidw	76	44	.	.	.
cord-345088-krb1eidw	77	1	To	to	TO
cord-345088-krb1eidw	77	2	map	map	VB
cord-345088-krb1eidw	77	3	the	the	DT
cord-345088-krb1eidw	77	4	defects	defect	NNS
cord-345088-krb1eidw	77	5	responsible	responsible	JJ
cord-345088-krb1eidw	77	6	for	for	IN
cord-345088-krb1eidw	77	7	the	the	DT
cord-345088-krb1eidw	77	8	ts	ts	NNP
cord-345088-krb1eidw	77	9	phenotype	phenotype	NN
cord-345088-krb1eidw	77	10	described	describe	VBN
cord-345088-krb1eidw	77	11	above	above	RB
cord-345088-krb1eidw	77	12	,	,	,
cord-345088-krb1eidw	77	13	the	the	DT
cord-345088-krb1eidw	77	14	complete	complete	JJ
cord-345088-krb1eidw	77	15	nucleotide	nucleotide	JJ
cord-345088-krb1eidw	77	16	sequences	sequence	NNS
cord-345088-krb1eidw	77	17	of	of	IN
cord-345088-krb1eidw	77	18	wt6501	wt6501	NNP
cord-345088-krb1eidw	77	19	,	,	,
cord-345088-krb1eidw	77	20	ts291602	ts291602	NNP
cord-345088-krb1eidw	77	21	,	,	,
cord-345088-krb1eidw	77	22	ts282902	ts282902	ADD
cord-345088-krb1eidw	77	23	,	,	,
cord-345088-krb1eidw	77	24	and	and	CC
cord-345088-krb1eidw	77	25	rev-1	rev-1	NNP
cord-345088-krb1eidw	77	26	were	be	VBD
cord-345088-krb1eidw	77	27	determined	determine	VBN
cord-345088-krb1eidw	77	28	using	use	VBG
cord-345088-krb1eidw	77	29	RT	RT	NNP
cord-345088-krb1eidw	77	30	-	-	HYPH
cord-345088-krb1eidw	77	31	PCR	PCR	NNP
cord-345088-krb1eidw	77	32	products	product	NNS
cord-345088-krb1eidw	77	33	and	and	CC
cord-345088-krb1eidw	77	34	cDNA	cdna	NN
cord-345088-krb1eidw	77	35	clones	clone	NNS
cord-345088-krb1eidw	77	36	from	from	IN
cord-345088-krb1eidw	77	37	each	each	DT
cord-345088-krb1eidw	77	38	virus	virus	NN
cord-345088-krb1eidw	77	39	.	.	.
cord-345088-krb1eidw	78	1	Comparison	comparison	NN
cord-345088-krb1eidw	78	2	of	of	IN
cord-345088-krb1eidw	78	3	the	the	DT
cord-345088-krb1eidw	78	4	sequence	sequence	NN
cord-345088-krb1eidw	78	5	of	of	IN
cord-345088-krb1eidw	78	6	wt6501	wt6501	NN
cord-345088-krb1eidw	78	7	with	with	IN
cord-345088-krb1eidw	78	8	ts291602	ts291602	NNP
cord-345088-krb1eidw	78	9	revealed	reveal	VBD
cord-345088-krb1eidw	78	10	22	22	CD
cord-345088-krb1eidw	78	11	point	point	NN
cord-345088-krb1eidw	78	12	mutations	mutation	NNS
cord-345088-krb1eidw	78	13	,	,	,
cord-345088-krb1eidw	78	14	a	a	DT
cord-345088-krb1eidw	78	15	5-base	5-base	CD
cord-345088-krb1eidw	78	16	-	-	HYPH
cord-345088-krb1eidw	78	17	deletion	deletion	NN
cord-345088-krb1eidw	78	18	,	,	,
cord-345088-krb1eidw	78	19	a	a	DT
cord-345088-krb1eidw	78	20	1-base-	1-base-	CD
cord-345088-krb1eidw	78	21	,	,	,
cord-345088-krb1eidw	78	22	3-base	3-base	CD
cord-345088-krb1eidw	78	23	-	-	:
cord-345088-krb1eidw	78	24	and	and	CC
cord-345088-krb1eidw	78	25	a	a	DT
cord-345088-krb1eidw	78	26	9-base	9-base	CD
cord-345088-krb1eidw	78	27	-	-	HYPH
cord-345088-krb1eidw	78	28	insertion	insertion	NN
cord-345088-krb1eidw	78	29	(	(	-LRB-
cord-345088-krb1eidw	78	30	Table	table	NN
cord-345088-krb1eidw	78	31	2	2	CD
cord-345088-krb1eidw	78	32	)	)	-RRB-
cord-345088-krb1eidw	78	33	.	.	.
cord-345088-krb1eidw	79	1	These	these	DT
cord-345088-krb1eidw	79	2	alterations	alteration	NNS
cord-345088-krb1eidw	79	3	caused	cause	VBD
cord-345088-krb1eidw	79	4	15	15	CD
cord-345088-krb1eidw	79	5	amino	amino	NN
cord-345088-krb1eidw	79	6	acid	acid	NN
cord-345088-krb1eidw	79	7	substitutions	substitution	NNS
cord-345088-krb1eidw	79	8	in	in	IN
cord-345088-krb1eidw	79	9	eight	eight	CD
cord-345088-krb1eidw	79	10	mature	mature	JJ
cord-345088-krb1eidw	79	11	viral	viral	JJ
cord-345088-krb1eidw	79	12	proteins	protein	NNS
cord-345088-krb1eidw	79	13	,	,	,
cord-345088-krb1eidw	79	14	a	a	DT
cord-345088-krb1eidw	79	15	single	single	JJ
cord-345088-krb1eidw	79	16	amino	amino	NN
cord-345088-krb1eidw	79	17	acid	acid	NN
cord-345088-krb1eidw	79	18	insertion	insertion	NN
cord-345088-krb1eidw	79	19	in	in	IN
cord-345088-krb1eidw	79	20	the	the	DT
cord-345088-krb1eidw	79	21	3a	3a	NNP
cord-345088-krb1eidw	79	22	protein	protein	NNP
cord-345088-krb1eidw	79	23	,	,	,
cord-345088-krb1eidw	79	24	a	a	DT
cord-345088-krb1eidw	79	25	truncated	truncated	JJ
cord-345088-krb1eidw	79	26	3b	3b	NN
cord-345088-krb1eidw	79	27	(	(	-LRB-
cord-345088-krb1eidw	79	28	compared	compare	VBN
cord-345088-krb1eidw	79	29	with	with	IN
cord-345088-krb1eidw	79	30	early	early	JJ
cord-345088-krb1eidw	79	31	passages	passage	NNS
cord-345088-krb1eidw	79	32	of	of	IN
cord-345088-krb1eidw	79	33	wt	wt	NN
cord-345088-krb1eidw	79	34	virus	virus	NN
cord-345088-krb1eidw	79	35	)	)	-RRB-
cord-345088-krb1eidw	79	36	caused	cause	VBN
cord-345088-krb1eidw	79	37	by	by	IN
cord-345088-krb1eidw	79	38	a	a	DT
cord-345088-krb1eidw	79	39	single	single	JJ
cord-345088-krb1eidw	79	40	base	base	NN
cord-345088-krb1eidw	79	41	insertion	insertion	NN
cord-345088-krb1eidw	79	42	in	in	IN
cord-345088-krb1eidw	79	43	the	the	DT
cord-345088-krb1eidw	79	44	3b	3b	CD
cord-345088-krb1eidw	79	45	of	of	IN
cord-345088-krb1eidw	79	46	ts291602	ts291602	NNP
cord-345088-krb1eidw	79	47	,	,	,
cord-345088-krb1eidw	79	48	and	and	CC
cord-345088-krb1eidw	79	49	a	a	DT
cord-345088-krb1eidw	79	50	3-amino	3-amino	CD
cord-345088-krb1eidw	79	51	acid	acid	NN
cord-345088-krb1eidw	79	52	insertion	insertion	NN
cord-345088-krb1eidw	79	53	in	in	IN
cord-345088-krb1eidw	79	54	the	the	DT
cord-345088-krb1eidw	79	55	N	n	CD
cord-345088-krb1eidw	79	56	protein	protein	NN
cord-345088-krb1eidw	79	57	.	.	.
cord-345088-krb1eidw	80	1	In	in	IN
cord-345088-krb1eidw	80	2	ts282902	ts282902	CD
cord-345088-krb1eidw	80	3	,	,	,
cord-345088-krb1eidw	80	4	similar	similar	JJ
cord-345088-krb1eidw	80	5	point	point	NN
cord-345088-krb1eidw	80	6	mutations	mutation	NNS
cord-345088-krb1eidw	80	7	,	,	,
cord-345088-krb1eidw	80	8	deletion	deletion	NN
cord-345088-krb1eidw	80	9	and	and	CC
cord-345088-krb1eidw	80	10	insertion	insertion	NN
cord-345088-krb1eidw	80	11	were	be	VBD
cord-345088-krb1eidw	80	12	found	find	VBN
cord-345088-krb1eidw	80	13	(	(	-LRB-
cord-345088-krb1eidw	80	14	Table	table	NN
cord-345088-krb1eidw	80	15	2	2	CD
cord-345088-krb1eidw	80	16	)	)	-RRB-
cord-345088-krb1eidw	80	17	.	.	.
cord-345088-krb1eidw	81	1	The	the	DT
cord-345088-krb1eidw	81	2	most	most	RBS
cord-345088-krb1eidw	81	3	different	different	JJ
cord-345088-krb1eidw	81	4	feature	feature	NN
cord-345088-krb1eidw	81	5	of	of	IN
cord-345088-krb1eidw	81	6	the	the	DT
cord-345088-krb1eidw	81	7	two	two	CD
cord-345088-krb1eidw	81	8	ts	ts	NNPS
cord-345088-krb1eidw	81	9	mutant	mutant	NN
cord-345088-krb1eidw	81	10	is	be	VBZ
cord-345088-krb1eidw	81	11	the	the	DT
cord-345088-krb1eidw	81	12	insertion	insertion	NN
cord-345088-krb1eidw	81	13	in	in	IN
cord-345088-krb1eidw	81	14	the	the	DT
cord-345088-krb1eidw	81	15	3b	3b	CD
cord-345088-krb1eidw	81	16	gene	gene	NN
cord-345088-krb1eidw	81	17	.	.	.
cord-345088-krb1eidw	82	1	In	in	IN
cord-345088-krb1eidw	82	2	ts291602	ts291602	NNP
cord-345088-krb1eidw	82	3	,	,	,
cord-345088-krb1eidw	82	4	a	a	DT
cord-345088-krb1eidw	82	5	single	single	JJ
cord-345088-krb1eidw	82	6	A	a	DT
cord-345088-krb1eidw	82	7	insertion	insertion	NN
cord-345088-krb1eidw	82	8	was	be	VBD
cord-345088-krb1eidw	82	9	found	find	VBN
cord-345088-krb1eidw	82	10	at	at	IN
cord-345088-krb1eidw	82	11	a	a	DT
cord-345088-krb1eidw	82	12	poly	poly	JJ
cord-345088-krb1eidw	82	13	-	-	HYPH
cord-345088-krb1eidw	82	14	A	a	DT
cord-345088-krb1eidw	82	15	stretch	stretch	NN
cord-345088-krb1eidw	82	16	at	at	IN
cord-345088-krb1eidw	82	17	positions	position	NNS
cord-345088-krb1eidw	82	18	24075	24075	CD
cord-345088-krb1eidw	82	19	to	to	IN
cord-345088-krb1eidw	82	20	24080	24080	CD
cord-345088-krb1eidw	82	21	,	,	,
cord-345088-krb1eidw	82	22	causing	cause	VBG
cord-345088-krb1eidw	82	23	frameshift	frameshift	NN
cord-345088-krb1eidw	82	24	of	of	IN
cord-345088-krb1eidw	82	25	the	the	DT
cord-345088-krb1eidw	82	26	3b	3b	NNP
cord-345088-krb1eidw	82	27	gene	gene	NN
cord-345088-krb1eidw	82	28	and	and	CC
cord-345088-krb1eidw	82	29	resulting	result	VBG
cord-345088-krb1eidw	82	30	in	in	IN
cord-345088-krb1eidw	82	31	a	a	DT
cord-345088-krb1eidw	82	32	C	c	NN
cord-345088-krb1eidw	82	33	-	-	HYPH
cord-345088-krb1eidw	82	34	terminally	terminally	RB
cord-345088-krb1eidw	82	35	truncated	truncate	VBN
cord-345088-krb1eidw	82	36	3b	3b	JJ
cord-345088-krb1eidw	82	37	protein	protein	NN
cord-345088-krb1eidw	82	38	(	(	-LRB-
cord-345088-krb1eidw	82	39	Shen	Shen	NNP
cord-345088-krb1eidw	82	40	and	and	CC
cord-345088-krb1eidw	82	41	Liu	Liu	NNP
cord-345088-krb1eidw	82	42	,	,	,
cord-345088-krb1eidw	82	43	2003	2003	CD
cord-345088-krb1eidw	82	44	)	)	-RRB-
cord-345088-krb1eidw	82	45	.	.	.
cord-345088-krb1eidw	83	1	However	however	RB
cord-345088-krb1eidw	83	2	,	,	,
cord-345088-krb1eidw	83	3	a	a	DT
cord-345088-krb1eidw	83	4	three	three	CD
cord-345088-krb1eidw	83	5	-	-	HYPH
cord-345088-krb1eidw	83	6	A	a	NN
cord-345088-krb1eidw	83	7	-	-	HYPH
cord-345088-krb1eidw	83	8	insertion	insertion	NN
cord-345088-krb1eidw	83	9	at	at	IN
cord-345088-krb1eidw	83	10	the	the	DT
cord-345088-krb1eidw	83	11	same	same	JJ
cord-345088-krb1eidw	83	12	poly	poly	JJ
cord-345088-krb1eidw	83	13	-	-	HYPH
cord-345088-krb1eidw	83	14	A	a	DT
cord-345088-krb1eidw	83	15	stretch	stretch	NN
cord-345088-krb1eidw	83	16	was	be	VBD
cord-345088-krb1eidw	83	17	found	find	VBN
cord-345088-krb1eidw	83	18	in	in	IN
cord-345088-krb1eidw	83	19	ts282902	ts282902	NN
cord-345088-krb1eidw	83	20	,	,	,
cord-345088-krb1eidw	83	21	which	which	WDT
cord-345088-krb1eidw	83	22	does	do	VBZ
cord-345088-krb1eidw	83	23	not	not	RB
cord-345088-krb1eidw	83	24	affect	affect	VB
cord-345088-krb1eidw	83	25	the	the	DT
cord-345088-krb1eidw	83	26	open	open	JJ
cord-345088-krb1eidw	83	27	reading	reading	NN
cord-345088-krb1eidw	83	28	frame	frame	NN
cord-345088-krb1eidw	83	29	of	of	IN
cord-345088-krb1eidw	83	30	the	the	DT
cord-345088-krb1eidw	83	31	3b	3b	NNP
cord-345088-krb1eidw	83	32	gene	gene	NN
cord-345088-krb1eidw	83	33	.	.	.
cord-345088-krb1eidw	84	1	Comparison	comparison	NN
cord-345088-krb1eidw	84	2	of	of	IN
cord-345088-krb1eidw	84	3	the	the	DT
cord-345088-krb1eidw	84	4	sequence	sequence	NN
cord-345088-krb1eidw	84	5	of	of	IN
cord-345088-krb1eidw	84	6	rev-1	rev-1	NNP
cord-345088-krb1eidw	84	7	with	with	IN
cord-345088-krb1eidw	84	8	the	the	DT
cord-345088-krb1eidw	84	9	two	two	CD
cord-345088-krb1eidw	84	10	ts	t	NNS
cord-345088-krb1eidw	84	11	mutants	mutant	NNS
cord-345088-krb1eidw	84	12	showed	show	VBD
cord-345088-krb1eidw	84	13	only	only	RB
cord-345088-krb1eidw	84	14	one	one	CD
cord-345088-krb1eidw	84	15	amino	amino	NN
cord-345088-krb1eidw	84	16	acid	acid	NN
cord-345088-krb1eidw	84	17	change	change	NN
cord-345088-krb1eidw	84	18	(	(	-LRB-
cord-345088-krb1eidw	84	19	Q	Q	NNP
cord-345088-krb1eidw	84	20	294	294	CD
cord-345088-krb1eidw	84	21	-to	-to	NNPS
cord-345088-krb1eidw	84	22	-	-	:
cord-345088-krb1eidw	84	23	L	L	NNP
cord-345088-krb1eidw	84	24	294	294	CD
cord-345088-krb1eidw	84	25	)	)	-RRB-
cord-345088-krb1eidw	84	26	in	in	IN
cord-345088-krb1eidw	84	27	the	the	DT
cord-345088-krb1eidw	84	28	S	S	NNP
cord-345088-krb1eidw	84	29	protein	protein	NN
cord-345088-krb1eidw	84	30	and	and	CC
cord-345088-krb1eidw	84	31	one	one	CD
cord-345088-krb1eidw	84	32	amino	amino	NN
cord-345088-krb1eidw	84	33	acid	acid	NN
cord-345088-krb1eidw	84	34	deletion	deletion	NN
cord-345088-krb1eidw	84	35	in	in	IN
cord-345088-krb1eidw	84	36	the	the	DT
cord-345088-krb1eidw	84	37	nonstructural	nonstructural	JJ
cord-345088-krb1eidw	84	38	protein	protein	NN
cord-345088-krb1eidw	84	39	3a	3a	NNP
cord-345088-krb1eidw	84	40	(	(	-LRB-
cord-345088-krb1eidw	84	41	I	I	NNP
cord-345088-krb1eidw	84	42	28	28	CD
cord-345088-krb1eidw	84	43	)	)	-RRB-
cord-345088-krb1eidw	84	44	,	,	,
cord-345088-krb1eidw	84	45	which	which	WDT
cord-345088-krb1eidw	84	46	exists	exist	VBZ
cord-345088-krb1eidw	84	47	in	in	IN
cord-345088-krb1eidw	84	48	both	both	CC
cord-345088-krb1eidw	84	49	ts291602	ts291602	NNP
cord-345088-krb1eidw	84	50	and	and	CC
cord-345088-krb1eidw	84	51	ts	ts	NNP
cord-345088-krb1eidw	84	52	282902	282902	CD
cord-345088-krb1eidw	84	53	but	but	CC
cord-345088-krb1eidw	84	54	not	not	RB
cord-345088-krb1eidw	84	55	in	in	IN
cord-345088-krb1eidw	84	56	rev-1	rev-1	NNP
cord-345088-krb1eidw	84	57	and	and	CC
cord-345088-krb1eidw	84	58	wt	wt	NN
cord-345088-krb1eidw	84	59	virus	virus	NN
cord-345088-krb1eidw	84	60	.	.	.
cord-345088-krb1eidw	85	1	The	the	DT
cord-345088-krb1eidw	85	2	genotype	genotype	NN
cord-345088-krb1eidw	85	3	of	of	IN
cord-345088-krb1eidw	85	4	the	the	DT
cord-345088-krb1eidw	85	5	rev-1	rev-1	NNP
cord-345088-krb1eidw	85	6	at	at	IN
cord-345088-krb1eidw	85	7	these	these	DT
cord-345088-krb1eidw	85	8	two	two	CD
cord-345088-krb1eidw	85	9	positions	position	NNS
cord-345088-krb1eidw	85	10	(	(	-LRB-
cord-345088-krb1eidw	85	11	Q	Q	NNP
cord-345088-krb1eidw	85	12	294	294	CD
cord-345088-krb1eidw	85	13	and	and	CC
cord-345088-krb1eidw	85	14	I	-PRON-	PRP
cord-345088-krb1eidw	85	15	28	28	CD
cord-345088-krb1eidw	85	16	)	)	-RRB-
cord-345088-krb1eidw	85	17	are	be	VBP
cord-345088-krb1eidw	85	18	therefore	therefore	RB
cord-345088-krb1eidw	85	19	the	the	DT
cord-345088-krb1eidw	85	20	same	same	JJ
cord-345088-krb1eidw	85	21	as	as	IN
cord-345088-krb1eidw	85	22	that	that	DT
cord-345088-krb1eidw	85	23	of	of	IN
cord-345088-krb1eidw	85	24	wt6501	wt6501	NN
cord-345088-krb1eidw	85	25	,	,	,
cord-345088-krb1eidw	85	26	but	but	CC
cord-345088-krb1eidw	85	27	different	different	JJ
cord-345088-krb1eidw	85	28	from	from	IN
cord-345088-krb1eidw	85	29	the	the	DT
cord-345088-krb1eidw	85	30	two	two	CD
cord-345088-krb1eidw	85	31	ts	ts	NNP
cord-345088-krb1eidw	85	32	mutants	mutant	NNS
cord-345088-krb1eidw	85	33	.	.	.
cord-345088-krb1eidw	86	1	As	as	IN
cord-345088-krb1eidw	86	2	both	both	DT
cord-345088-krb1eidw	86	3	wt	wt	NN
cord-345088-krb1eidw	86	4	virus	virus	NN
cord-345088-krb1eidw	86	5	and	and	CC
cord-345088-krb1eidw	86	6	the	the	DT
cord-345088-krb1eidw	86	7	revertant	revertant	NN
cord-345088-krb1eidw	86	8	could	could	MD
cord-345088-krb1eidw	86	9	form	form	VB
cord-345088-krb1eidw	86	10	plaques	plaque	NNS
cord-345088-krb1eidw	86	11	at	at	IN
cord-345088-krb1eidw	86	12	32	32	CD
cord-345088-krb1eidw	86	13	and	and	CC
cord-345088-krb1eidw	86	14	40	40	CD
cord-345088-krb1eidw	86	15	jC	jC	NNS
cord-345088-krb1eidw	86	16	,	,	,
cord-345088-krb1eidw	86	17	these	these	DT
cord-345088-krb1eidw	86	18	two	two	CD
cord-345088-krb1eidw	86	19	mutations	mutation	NNS
cord-345088-krb1eidw	86	20	might	may	MD
cord-345088-krb1eidw	86	21	be	be	VB
cord-345088-krb1eidw	86	22	responsible	responsible	JJ
cord-345088-krb1eidw	86	23	for	for	IN
cord-345088-krb1eidw	86	24	the	the	DT
cord-345088-krb1eidw	86	25	ts	ts	NNP
cord-345088-krb1eidw	86	26	phenotype	phenotype	NN
cord-345088-krb1eidw	86	27	.	.	.
cord-345088-krb1eidw	87	1	A	a	DT
cord-345088-krb1eidw	87	2	Blast	blast	NN
cord-345088-krb1eidw	87	3	search	search	NN
cord-345088-krb1eidw	87	4	of	of	IN
cord-345088-krb1eidw	87	5	GenBank	GenBank	NNP
cord-345088-krb1eidw	87	6	clearly	clearly	RB
cord-345088-krb1eidw	87	7	showed	show	VBD
cord-345088-krb1eidw	87	8	that	that	IN
cord-345088-krb1eidw	87	9	the	the	DT
cord-345088-krb1eidw	87	10	amino	amino	NN
cord-345088-krb1eidw	87	11	acid	acid	NN
cord-345088-krb1eidw	87	12	at	at	IN
cord-345088-krb1eidw	87	13	position	position	NN
cord-345088-krb1eidw	87	14	294	294	CD
cord-345088-krb1eidw	87	15	of	of	IN
cord-345088-krb1eidw	87	16	the	the	DT
cord-345088-krb1eidw	87	17	S	S	NNP
cord-345088-krb1eidw	87	18	protein	protein	NN
cord-345088-krb1eidw	87	19	was	be	VBD
cord-345088-krb1eidw	87	20	a	a	DT
cord-345088-krb1eidw	87	21	conserved	conserved	JJ
cord-345088-krb1eidw	87	22	glutamine	glutamine	NN
cord-345088-krb1eidw	87	23	.	.	.
cord-345088-krb1eidw	88	1	The	the	DT
cord-345088-krb1eidw	88	2	Leu	Leu	NNP
cord-345088-krb1eidw	88	3	294	294	CD
cord-345088-krb1eidw	88	4	residue	residue	NN
cord-345088-krb1eidw	88	5	in	in	IN
cord-345088-krb1eidw	88	6	the	the	DT
cord-345088-krb1eidw	88	7	two	two	CD
cord-345088-krb1eidw	88	8	ts	t	NNS
cord-345088-krb1eidw	88	9	mutants	mutant	NNS
cord-345088-krb1eidw	88	10	is	be	VBZ
cord-345088-krb1eidw	88	11	located	locate	VBN
cord-345088-krb1eidw	88	12	immediately	immediately	RB
cord-345088-krb1eidw	88	13	downstream	downstream	RB
cord-345088-krb1eidw	88	14	of	of	IN
cord-345088-krb1eidw	88	15	a	a	DT
cord-345088-krb1eidw	88	16	hypervariable	hypervariable	JJ
cord-345088-krb1eidw	88	17	region	region	NN
cord-345088-krb1eidw	88	18	and	and	CC
cord-345088-krb1eidw	88	19	the	the	DT
cord-345088-krb1eidw	88	20	upstream	upstream	NN
cord-345088-krb1eidw	88	21	of	of	IN
cord-345088-krb1eidw	88	22	a	a	DT
cord-345088-krb1eidw	88	23	conserved	conserved	JJ
cord-345088-krb1eidw	88	24	region	region	NN
cord-345088-krb1eidw	88	25	of	of	IN
cord-345088-krb1eidw	88	26	the	the	DT
cord-345088-krb1eidw	88	27	S1	S1	NNP
cord-345088-krb1eidw	88	28	subunit	subunit	NN
cord-345088-krb1eidw	88	29	.	.	.
cord-345088-krb1eidw	89	1	One	one	CD
cord-345088-krb1eidw	89	2	additional	additional	JJ
cord-345088-krb1eidw	89	3	mutation	mutation	NN
cord-345088-krb1eidw	89	4	,	,	,
cord-345088-krb1eidw	89	5	D	D	NNP
cord-345088-krb1eidw	89	6	709	709	CD
cord-345088-krb1eidw	89	7	-N	-n	SYM
cord-345088-krb1eidw	89	8	709	709	CD
cord-345088-krb1eidw	89	9	,	,	,
cord-345088-krb1eidw	89	10	was	be	VBD
cord-345088-krb1eidw	89	11	found	find	VBN
cord-345088-krb1eidw	89	12	in	in	IN
cord-345088-krb1eidw	89	13	ts291602	ts291602	NNP
cord-345088-krb1eidw	89	14	only	only	RB
cord-345088-krb1eidw	89	15	,	,	,
cord-345088-krb1eidw	89	16	and	and	CC
cord-345088-krb1eidw	89	17	an	an	DT
cord-345088-krb1eidw	89	18	I	i	NN
cord-345088-krb1eidw	89	19	769	769	CD
cord-345088-krb1eidw	89	20	-M	-M	:
cord-345088-krb1eidw	89	21	769	769	CD
cord-345088-krb1eidw	89	22	mutation	mutation	NN
cord-345088-krb1eidw	89	23	in	in	IN
cord-345088-krb1eidw	89	24	the	the	DT
cord-345088-krb1eidw	89	25	two	two	CD
cord-345088-krb1eidw	89	26	ts	ts	NN
cord-345088-krb1eidw	89	27	mutants	mutant	NNS
cord-345088-krb1eidw	89	28	and	and	CC
cord-345088-krb1eidw	89	29	rev-1	rev-1	NNP
cord-345088-krb1eidw	89	30	.	.	.
cord-345088-krb1eidw	90	1	After	after	IN
cord-345088-krb1eidw	90	2	mapping	map	VBG
cord-345088-krb1eidw	90	3	the	the	DT
cord-345088-krb1eidw	90	4	potential	potential	JJ
cord-345088-krb1eidw	90	5	determinants	determinant	NNS
cord-345088-krb1eidw	90	6	responsible	responsible	JJ
cord-345088-krb1eidw	90	7	for	for	IN
cord-345088-krb1eidw	90	8	the	the	DT
cord-345088-krb1eidw	90	9	ts	ts	NNP
cord-345088-krb1eidw	90	10	phenotype	phenotype	NN
cord-345088-krb1eidw	90	11	,	,	,
cord-345088-krb1eidw	90	12	we	-PRON-	PRP
cord-345088-krb1eidw	90	13	would	would	MD
cord-345088-krb1eidw	90	14	like	like	VB
cord-345088-krb1eidw	90	15	to	to	TO
cord-345088-krb1eidw	90	16	explore	explore	VB
cord-345088-krb1eidw	90	17	the	the	DT
cord-345088-krb1eidw	90	18	mechanisms	mechanism	NNS
cord-345088-krb1eidw	90	19	by	by	IN
cord-345088-krb1eidw	90	20	which	which	WDT
cord-345088-krb1eidw	90	21	these	these	DT
cord-345088-krb1eidw	90	22	mutations	mutation	NNS
cord-345088-krb1eidw	90	23	affect	affect	VBP
cord-345088-krb1eidw	90	24	the	the	DT
cord-345088-krb1eidw	90	25	propagation	propagation	NN
cord-345088-krb1eidw	90	26	of	of	IN
cord-345088-krb1eidw	90	27	the	the	DT
cord-345088-krb1eidw	90	28	ts	ts	NNP
cord-345088-krb1eidw	90	29	mutants	mutant	NNS
cord-345088-krb1eidw	90	30	at	at	IN
cord-345088-krb1eidw	90	31	the	the	DT
cord-345088-krb1eidw	90	32	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	90	33	temperature	temperature	NN
cord-345088-krb1eidw	90	34	.	.	.
cord-345088-krb1eidw	91	1	Synthesis	synthesis	NN
cord-345088-krb1eidw	91	2	of	of	IN
cord-345088-krb1eidw	91	3	the	the	DT
cord-345088-krb1eidw	91	4	S	S	NNP
cord-345088-krb1eidw	91	5	protein	protein	NN
cord-345088-krb1eidw	91	6	in	in	IN
cord-345088-krb1eidw	91	7	cells	cell	NNS
cord-345088-krb1eidw	91	8	infected	infect	VBN
cord-345088-krb1eidw	91	9	with	with	IN
cord-345088-krb1eidw	91	10	wt	wt	NN
cord-345088-krb1eidw	91	11	virus	virus	NN
cord-345088-krb1eidw	91	12	,	,	,
cord-345088-krb1eidw	91	13	ts	ts	NNP
cord-345088-krb1eidw	91	14	mutants	mutant	NNS
cord-345088-krb1eidw	91	15	,	,	,
cord-345088-krb1eidw	91	16	and	and	CC
cord-345088-krb1eidw	91	17	revertant	revertant	NNP
cord-345088-krb1eidw	91	18	was	be	VBD
cord-345088-krb1eidw	91	19	analyzed	analyze	VBN
cord-345088-krb1eidw	91	20	.	.	.
cord-345088-krb1eidw	92	1	Two	two	CD
cord-345088-krb1eidw	92	2	sets	set	NNS
cord-345088-krb1eidw	92	3	of	of	IN
cord-345088-krb1eidw	92	4	experiments	experiment	NNS
cord-345088-krb1eidw	92	5	were	be	VBD
cord-345088-krb1eidw	92	6	carried	carry	VBN
cord-345088-krb1eidw	92	7	out	out	RP
cord-345088-krb1eidw	92	8	.	.	.
cord-345088-krb1eidw	93	1	First	first	RB
cord-345088-krb1eidw	93	2	,	,	,
cord-345088-krb1eidw	93	3	Vero	Vero	NNP
cord-345088-krb1eidw	93	4	cells	cell	NNS
cord-345088-krb1eidw	93	5	were	be	VBD
cord-345088-krb1eidw	93	6	infected	infect	VBN
cord-345088-krb1eidw	93	7	with	with	IN
cord-345088-krb1eidw	93	8	wt6501	wt6501	NNP
cord-345088-krb1eidw	93	9	,	,	,
cord-345088-krb1eidw	93	10	ts291602	ts291602	NNP
cord-345088-krb1eidw	93	11	,	,	,
cord-345088-krb1eidw	93	12	ts282902	ts282902	ADD
cord-345088-krb1eidw	93	13	,	,	,
cord-345088-krb1eidw	93	14	and	and	CC
cord-345088-krb1eidw	93	15	rev-1	rev-1	NNP
cord-345088-krb1eidw	93	16	at	at	IN
cord-345088-krb1eidw	93	17	32	32	CD
cord-345088-krb1eidw	93	18	and	and	CC
cord-345088-krb1eidw	93	19	40	40	CD
cord-345088-krb1eidw	93	20	jC	jC	NNS
cord-345088-krb1eidw	93	21	,	,	,
cord-345088-krb1eidw	93	22	respectively	respectively	RB
cord-345088-krb1eidw	93	23	,	,	,
cord-345088-krb1eidw	93	24	and	and	CC
cord-345088-krb1eidw	93	25	lysates	lysates	NNP
cord-345088-krb1eidw	93	26	were	be	VBD
cord-345088-krb1eidw	93	27	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	93	28	with	with	IN
cord-345088-krb1eidw	93	29	anti	anti	JJ
cord-345088-krb1eidw	93	30	-	-	JJ
cord-345088-krb1eidw	93	31	IBV	IBV	NNP
cord-345088-krb1eidw	93	32	antibodies	antibody	NNS
cord-345088-krb1eidw	93	33	.	.	.
cord-345088-krb1eidw	94	1	It	-PRON-	PRP
cord-345088-krb1eidw	94	2	was	be	VBD
cord-345088-krb1eidw	94	3	obvious	obvious	JJ
cord-345088-krb1eidw	94	4	that	that	IN
cord-345088-krb1eidw	94	5	synthesis	synthesis	NN
cord-345088-krb1eidw	94	6	of	of	IN
cord-345088-krb1eidw	94	7	the	the	DT
cord-345088-krb1eidw	94	8	ts	ts	NNP
cord-345088-krb1eidw	94	9	mutant	mutant	NN
cord-345088-krb1eidw	94	10	S	S	NNP
cord-345088-krb1eidw	94	11	 	 	_SP
cord-345088-krb1eidw	94	12	Intergenic	Intergenic	NNP
cord-345088-krb1eidw	94	13	region	region	NN
cord-345088-krb1eidw	94	14	between	between	IN
cord-345088-krb1eidw	94	15	M	M	NNP
cord-345088-krb1eidw	94	16	and	and	CC
cord-345088-krb1eidw	94	17	5a	5a	CD
cord-345088-krb1eidw	94	18	25,346	25,346	CD
cord-345088-krb1eidw	94	19	Table	table	NN
cord-345088-krb1eidw	94	20	1	1	CD
cord-345088-krb1eidw	94	21	Titers	Titers	NNPS
cord-345088-krb1eidw	94	22	of	of	IN
cord-345088-krb1eidw	94	23	wild	wild	JJ
cord-345088-krb1eidw	94	24	type	type	NN
cord-345088-krb1eidw	94	25	,	,	,
cord-345088-krb1eidw	94	26	mutant	mutant	JJ
cord-345088-krb1eidw	94	27	and	and	CC
cord-345088-krb1eidw	94	28	revertant	revertant	JJ
cord-345088-krb1eidw	94	29	viruses	virus	NNS
cord-345088-krb1eidw	94	30	at	at	IN
cord-345088-krb1eidw	94	31	32	32	CD
cord-345088-krb1eidw	94	32	jC	jC	NNS
cord-345088-krb1eidw	94	33	and	and	CC
cord-345088-krb1eidw	95	1	40	40	CD
cord-345088-krb1eidw	95	2	jC	jC	NNS
cord-345088-krb1eidw	96	1	(	(	-LRB-
cord-345088-krb1eidw	96	2	PFU	pfu	NN
cord-345088-krb1eidw	96	3	/	/	SYM
cord-345088-krb1eidw	96	4	ml	ml	NN
cord-345088-krb1eidw	96	5	)	)	-RRB-
cord-345088-krb1eidw	97	1	Titer	titer	NN
cord-345088-krb1eidw	97	2	32	32	CD
cord-345088-krb1eidw	98	1	jC	jC	NNP
cord-345088-krb1eidw	98	2	4	4	CD
cord-345088-krb1eidw	98	3	0	0	CD
cord-345088-krb1eidw	99	1	jC	jC	NNP
cord-345088-krb1eidw	99	2	wt6501	wt6501	NNP
cord-345088-krb1eidw	99	3	8.5	8.5	CD
cord-345088-krb1eidw	99	4	Â	â	CD
cord-345088-krb1eidw	99	5	10	10	CD
cord-345088-krb1eidw	99	6	3	3	CD
cord-345088-krb1eidw	99	7	5.5	5.5	CD
cord-345088-krb1eidw	99	8	Â	â	CD
cord-345088-krb1eidw	99	9	10	10	CD
cord-345088-krb1eidw	99	10	6	6	CD
cord-345088-krb1eidw	99	11	ts291602	ts291602	NN
cord-345088-krb1eidw	99	12	2.0	2.0	CD
cord-345088-krb1eidw	99	13	Â	â	CD
cord-345088-krb1eidw	99	14	10	10	CD
cord-345088-krb1eidw	99	15	6	6	CD
cord-345088-krb1eidw	99	16	0	0	CD
cord-345088-krb1eidw	99	17	ts282902	ts282902	XX
cord-345088-krb1eidw	100	1	3.2	3.2	CD
cord-345088-krb1eidw	100	2	Â	â	CD
cord-345088-krb1eidw	100	3	10	10	CD
cord-345088-krb1eidw	100	4	6	6	CD
cord-345088-krb1eidw	100	5	0	0	CD
cord-345088-krb1eidw	101	1	rev-1	rev-1	NNP
cord-345088-krb1eidw	102	1	7.0	7.0	CD
cord-345088-krb1eidw	102	2	Â	â	CD
cord-345088-krb1eidw	102	3	10	10	CD
cord-345088-krb1eidw	103	1	6	6	CD
cord-345088-krb1eidw	103	2	5.5	5.5	CD
cord-345088-krb1eidw	103	3	Â	â	CD
cord-345088-krb1eidw	103	4	10	10	CD
cord-345088-krb1eidw	103	5	6	6	CD
cord-345088-krb1eidw	103	6	protein	protein	NN
cord-345088-krb1eidw	103	7	was	be	VBD
cord-345088-krb1eidw	103	8	detectable	detectable	JJ
cord-345088-krb1eidw	103	9	at	at	IN
cord-345088-krb1eidw	103	10	32	32	CD
cord-345088-krb1eidw	103	11	jC	jC	NNS
cord-345088-krb1eidw	104	1	but	but	CC
cord-345088-krb1eidw	104	2	not	not	RB
cord-345088-krb1eidw	104	3	at	at	IN
cord-345088-krb1eidw	104	4	40	40	CD
cord-345088-krb1eidw	105	1	jC	jC	NNP
cord-345088-krb1eidw	106	1	(	(	-LRB-
cord-345088-krb1eidw	106	2	Fig	Fig	NNP
cord-345088-krb1eidw	106	3	.	.	.
cord-345088-krb1eidw	106	4	1a	1a	CD
cord-345088-krb1eidw	106	5	,	,	,
cord-345088-krb1eidw	106	6	lanes	lane	VBZ
cord-345088-krb1eidw	106	7	3	3	CD
cord-345088-krb1eidw	106	8	,	,	,
cord-345088-krb1eidw	106	9	4	4	CD
cord-345088-krb1eidw	106	10	,	,	,
cord-345088-krb1eidw	106	11	5	5	CD
cord-345088-krb1eidw	106	12	,	,	,
cord-345088-krb1eidw	106	13	and	and	CC
cord-345088-krb1eidw	106	14	6	6	CD
cord-345088-krb1eidw	106	15	)	)	-RRB-
cord-345088-krb1eidw	106	16	under	under	IN
cord-345088-krb1eidw	106	17	these	these	DT
cord-345088-krb1eidw	106	18	conditions	condition	NNS
cord-345088-krb1eidw	106	19	.	.	.
cord-345088-krb1eidw	107	1	In	in	IN
cord-345088-krb1eidw	107	2	contrast	contrast	NN
cord-345088-krb1eidw	107	3	,	,	,
cord-345088-krb1eidw	107	4	expression	expression	NN
cord-345088-krb1eidw	107	5	of	of	IN
cord-345088-krb1eidw	107	6	the	the	DT
cord-345088-krb1eidw	107	7	S	S	NNP
cord-345088-krb1eidw	107	8	protein	protein	NN
cord-345088-krb1eidw	107	9	was	be	VBD
cord-345088-krb1eidw	107	10	observed	observe	VBN
cord-345088-krb1eidw	107	11	in	in	IN
cord-345088-krb1eidw	107	12	cells	cell	NNS
cord-345088-krb1eidw	107	13	infected	infect	VBN
cord-345088-krb1eidw	107	14	with	with	IN
cord-345088-krb1eidw	107	15	wt6501	wt6501	NN
cord-345088-krb1eidw	107	16	and	and	CC
cord-345088-krb1eidw	107	17	rev-1	rev-1	NNP
cord-345088-krb1eidw	107	18	at	at	IN
cord-345088-krb1eidw	107	19	both	both	DT
cord-345088-krb1eidw	107	20	temperatures	temperature	NNS
cord-345088-krb1eidw	107	21	(	(	-LRB-
cord-345088-krb1eidw	107	22	Fig	Fig	NNP
cord-345088-krb1eidw	107	23	.	.	.
cord-345088-krb1eidw	107	24	1a	1a	CD
cord-345088-krb1eidw	107	25	,	,	,
cord-345088-krb1eidw	107	26	lanes	lane	NNS
cord-345088-krb1eidw	107	27	1	1	CD
cord-345088-krb1eidw	107	28	,	,	,
cord-345088-krb1eidw	107	29	2	2	CD
cord-345088-krb1eidw	107	30	,	,	,
cord-345088-krb1eidw	107	31	7	7	CD
cord-345088-krb1eidw	107	32	,	,	,
cord-345088-krb1eidw	107	33	and	and	CC
cord-345088-krb1eidw	107	34	8)	8)	NN
cord-345088-krb1eidw	107	35	,	,	,
cord-345088-krb1eidw	107	36	though	though	IN
cord-345088-krb1eidw	107	37	the	the	DT
cord-345088-krb1eidw	107	38	level	level	NN
cord-345088-krb1eidw	107	39	of	of	IN
cord-345088-krb1eidw	107	40	the	the	DT
cord-345088-krb1eidw	107	41	proteins	protein	NNS
cord-345088-krb1eidw	107	42	detected	detect	VBN
cord-345088-krb1eidw	107	43	in	in	IN
cord-345088-krb1eidw	107	44	cells	cell	NNS
cord-345088-krb1eidw	107	45	infected	infect	VBN
cord-345088-krb1eidw	107	46	with	with	IN
cord-345088-krb1eidw	107	47	wt6501	wt6501	NN
cord-345088-krb1eidw	107	48	was	be	VBD
cord-345088-krb1eidw	107	49	much	much	RB
cord-345088-krb1eidw	107	50	lower	low	JJR
cord-345088-krb1eidw	107	51	at	at	IN
cord-345088-krb1eidw	107	52	32	32	CD
cord-345088-krb1eidw	107	53	jC.	jc.	NN
cord-345088-krb1eidw	108	1	This	this	DT
cord-345088-krb1eidw	108	2	was	be	VBD
cord-345088-krb1eidw	108	3	consistent	consistent	JJ
cord-345088-krb1eidw	108	4	with	with	IN
cord-345088-krb1eidw	108	5	the	the	DT
cord-345088-krb1eidw	108	6	lower	low	JJR
cord-345088-krb1eidw	108	7	titers	titer	NNS
cord-345088-krb1eidw	108	8	of	of	IN
cord-345088-krb1eidw	108	9	wt	wt	NN
cord-345088-krb1eidw	108	10	virus	virus	NN
cord-345088-krb1eidw	108	11	at	at	IN
cord-345088-krb1eidw	108	12	32	32	CD
cord-345088-krb1eidw	108	13	jC.	jc.	NN
cord-345088-krb1eidw	109	1	In	in	IN
cord-345088-krb1eidw	109	2	the	the	DT
cord-345088-krb1eidw	109	3	second	second	JJ
cord-345088-krb1eidw	109	4	set	set	NN
cord-345088-krb1eidw	109	5	of	of	IN
cord-345088-krb1eidw	109	6	experiments	experiment	NNS
cord-345088-krb1eidw	109	7	shown	show	VBN
cord-345088-krb1eidw	109	8	in	in	IN
cord-345088-krb1eidw	109	9	Fig	Fig	NNP
cord-345088-krb1eidw	109	10	.	.	.
cord-345088-krb1eidw	110	1	1b	1b	LS
cord-345088-krb1eidw	110	2	,	,	,
cord-345088-krb1eidw	110	3	cells	cell	NNS
cord-345088-krb1eidw	110	4	were	be	VBD
cord-345088-krb1eidw	110	5	absorbed	absorb	VBN
cord-345088-krb1eidw	110	6	with	with	IN
cord-345088-krb1eidw	110	7	each	each	DT
cord-345088-krb1eidw	110	8	virus	virus	NN
cord-345088-krb1eidw	110	9	at	at	IN
cord-345088-krb1eidw	110	10	32	32	CD
cord-345088-krb1eidw	110	11	jC	jC	NNP
cord-345088-krb1eidw	110	12	for	for	IN
cord-345088-krb1eidw	110	13	1	1	CD
cord-345088-krb1eidw	110	14	h	h	NN
cord-345088-krb1eidw	110	15	and	and	CC
cord-345088-krb1eidw	110	16	one	one	CD
cord-345088-krb1eidw	110	17	of	of	IN
cord-345088-krb1eidw	110	18	the	the	DT
cord-345088-krb1eidw	110	19	duplicates	duplicate	NNS
cord-345088-krb1eidw	110	20	was	be	VBD
cord-345088-krb1eidw	110	21	shifted	shift	VBN
cord-345088-krb1eidw	110	22	to	to	IN
cord-345088-krb1eidw	110	23	40	40	CD
cord-345088-krb1eidw	110	24	jC.	jC.	NNS
cord-345088-krb1eidw	110	25	Under	under	IN
cord-345088-krb1eidw	110	26	these	these	DT
cord-345088-krb1eidw	110	27	conditions	condition	NNS
cord-345088-krb1eidw	110	28	,	,	,
cord-345088-krb1eidw	110	29	the	the	DT
cord-345088-krb1eidw	110	30	two	two	CD
cord-345088-krb1eidw	110	31	ts	ts	NN
cord-345088-krb1eidw	110	32	mutant	mutant	NN
cord-345088-krb1eidw	110	33	S	S	NNP
cord-345088-krb1eidw	110	34	proteins	protein	NNS
cord-345088-krb1eidw	110	35	were	be	VBD
cord-345088-krb1eidw	110	36	synthesized	synthesize	VBN
cord-345088-krb1eidw	110	37	at	at	IN
cord-345088-krb1eidw	110	38	both	both	DT
cord-345088-krb1eidw	110	39	32	32	CD
cord-345088-krb1eidw	110	40	and	and	CC
cord-345088-krb1eidw	110	41	40	40	CD
cord-345088-krb1eidw	110	42	jC	jC	NNS
cord-345088-krb1eidw	111	1	(	(	-LRB-
cord-345088-krb1eidw	111	2	Fig	Fig	NNP
cord-345088-krb1eidw	111	3	.	.	.
cord-345088-krb1eidw	111	4	1b	1b	CD
cord-345088-krb1eidw	111	5	,	,	,
cord-345088-krb1eidw	111	6	lanes	lane	VBZ
cord-345088-krb1eidw	111	7	3	3	CD
cord-345088-krb1eidw	111	8	,	,	,
cord-345088-krb1eidw	111	9	4	4	CD
cord-345088-krb1eidw	111	10	,	,	,
cord-345088-krb1eidw	111	11	5	5	CD
cord-345088-krb1eidw	111	12	,	,	,
cord-345088-krb1eidw	111	13	and	and	CC
cord-345088-krb1eidw	111	14	6	6	CD
cord-345088-krb1eidw	111	15	)	)	-RRB-
cord-345088-krb1eidw	111	16	,	,	,
cord-345088-krb1eidw	111	17	like	like	IN
cord-345088-krb1eidw	111	18	those	those	DT
cord-345088-krb1eidw	111	19	of	of	IN
cord-345088-krb1eidw	111	20	the	the	DT
cord-345088-krb1eidw	111	21	wt	wt	NN
cord-345088-krb1eidw	111	22	virus	virus	NN
cord-345088-krb1eidw	111	23	and	and	CC
cord-345088-krb1eidw	111	24	revertant	revertant	NN
cord-345088-krb1eidw	112	1	(	(	-LRB-
cord-345088-krb1eidw	112	2	Fig	Fig	NNP
cord-345088-krb1eidw	112	3	.	.	.
cord-345088-krb1eidw	112	4	1b	1b	CD
cord-345088-krb1eidw	112	5	,	,	,
cord-345088-krb1eidw	112	6	lanes	lane	NNS
cord-345088-krb1eidw	112	7	1	1	CD
cord-345088-krb1eidw	112	8	,	,	,
cord-345088-krb1eidw	112	9	2	2	CD
cord-345088-krb1eidw	112	10	,	,	,
cord-345088-krb1eidw	112	11	7	7	CD
cord-345088-krb1eidw	112	12	and	and	CC
cord-345088-krb1eidw	112	13	8)	8)	NN
cord-345088-krb1eidw	112	14	.	.	.
cord-345088-krb1eidw	113	1	These	these	DT
cord-345088-krb1eidw	113	2	results	result	NNS
cord-345088-krb1eidw	113	3	indicated	indicate	VBD
cord-345088-krb1eidw	113	4	that	that	IN
cord-345088-krb1eidw	113	5	the	the	DT
cord-345088-krb1eidw	113	6	viral	viral	JJ
cord-345088-krb1eidw	113	7	structural	structural	JJ
cord-345088-krb1eidw	113	8	protein	protein	NN
cord-345088-krb1eidw	113	9	could	could	MD
cord-345088-krb1eidw	113	10	be	be	VB
cord-345088-krb1eidw	113	11	synthesized	synthesize	VBN
cord-345088-krb1eidw	113	12	at	at	IN
cord-345088-krb1eidw	113	13	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	113	14	temperature	temperature	NN
cord-345088-krb1eidw	113	15	if	if	IN
cord-345088-krb1eidw	113	16	the	the	DT
cord-345088-krb1eidw	113	17	mutants	mutant	NNS
cord-345088-krb1eidw	113	18	were	be	VBD
cord-345088-krb1eidw	113	19	allowed	allow	VBN
cord-345088-krb1eidw	113	20	to	to	TO
cord-345088-krb1eidw	113	21	enter	enter	VB
cord-345088-krb1eidw	113	22	cells	cell	NNS
cord-345088-krb1eidw	113	23	.	.	.
cord-345088-krb1eidw	114	1	The	the	DT
cord-345088-krb1eidw	114	2	viral	viral	JJ
cord-345088-krb1eidw	114	3	subgenomic	subgenomic	NNP
cord-345088-krb1eidw	114	4	RNA	RNA	NNP
cord-345088-krb1eidw	114	5	and	and	CC
cord-345088-krb1eidw	114	6	one	one	CD
cord-345088-krb1eidw	114	7	of	of	IN
cord-345088-krb1eidw	114	8	the	the	DT
cord-345088-krb1eidw	114	9	processed	process	VBN
cord-345088-krb1eidw	114	10	proteins	protein	NNS
cord-345088-krb1eidw	114	11	,	,	,
cord-345088-krb1eidw	114	12	the	the	DT
cord-345088-krb1eidw	114	13	3C	3c	NN
cord-345088-krb1eidw	114	14	-	-	HYPH
cord-345088-krb1eidw	114	15	like	like	JJ
cord-345088-krb1eidw	114	16	proteinase	proteinase	NN
cord-345088-krb1eidw	114	17	,	,	,
cord-345088-krb1eidw	114	18	were	be	VBD
cord-345088-krb1eidw	114	19	also	also	RB
cord-345088-krb1eidw	114	20	detected	detect	VBN
cord-345088-krb1eidw	114	21	in	in	IN
cord-345088-krb1eidw	114	22	the	the	DT
cord-345088-krb1eidw	114	23	same	same	JJ
cord-345088-krb1eidw	114	24	ways	way	NNS
cord-345088-krb1eidw	114	25	(	(	-LRB-
cord-345088-krb1eidw	114	26	data	datum	NNS
cord-345088-krb1eidw	114	27	not	not	RB
cord-345088-krb1eidw	114	28	shown	show	VBN
cord-345088-krb1eidw	114	29	)	)	-RRB-
cord-345088-krb1eidw	114	30	,	,	,
cord-345088-krb1eidw	114	31	and	and	CC
cord-345088-krb1eidw	114	32	the	the	DT
cord-345088-krb1eidw	114	33	results	result	NNS
cord-345088-krb1eidw	114	34	were	be	VBD
cord-345088-krb1eidw	114	35	consistent	consistent	JJ
cord-345088-krb1eidw	114	36	with	with	IN
cord-345088-krb1eidw	114	37	those	those	DT
cord-345088-krb1eidw	114	38	described	describe	VBN
cord-345088-krb1eidw	114	39	above	above	RB
cord-345088-krb1eidw	114	40	.	.	.
cord-345088-krb1eidw	115	1	Comparison	comparison	NN
cord-345088-krb1eidw	115	2	of	of	IN
cord-345088-krb1eidw	115	3	the	the	DT
cord-345088-krb1eidw	115	4	nucleotide	nucleotide	JJ
cord-345088-krb1eidw	115	5	sequences	sequence	NNS
cord-345088-krb1eidw	115	6	and	and	CC
cord-345088-krb1eidw	115	7	the	the	DT
cord-345088-krb1eidw	115	8	S	S	NNP
cord-345088-krb1eidw	115	9	protein	protein	NN
cord-345088-krb1eidw	115	10	synthesis	synthesis	NN
cord-345088-krb1eidw	115	11	of	of	IN
cord-345088-krb1eidw	115	12	wt	wt	NN
cord-345088-krb1eidw	115	13	,	,	,
cord-345088-krb1eidw	115	14	ts	ts	NNP
cord-345088-krb1eidw	115	15	mutants	mutant	NNS
cord-345088-krb1eidw	115	16	and	and	CC
cord-345088-krb1eidw	115	17	revertant	revertant	NN
cord-345088-krb1eidw	115	18	indicate	indicate	VBP
cord-345088-krb1eidw	115	19	that	that	IN
cord-345088-krb1eidw	115	20	the	the	DT
cord-345088-krb1eidw	115	21	single	single	JJ
cord-345088-krb1eidw	115	22	amino	amino	NN
cord-345088-krb1eidw	115	23	acid	acid	NN
cord-345088-krb1eidw	115	24	mutation	mutation	NN
cord-345088-krb1eidw	115	25	(	(	-LRB-
cord-345088-krb1eidw	115	26	Q	Q	NNP
cord-345088-krb1eidw	115	27	294	294	CD
cord-345088-krb1eidw	115	28	-L	-l	SYM
cord-345088-krb1eidw	115	29	294	294	CD
cord-345088-krb1eidw	115	30	)	)	-RRB-
cord-345088-krb1eidw	115	31	in	in	IN
cord-345088-krb1eidw	115	32	the	the	DT
cord-345088-krb1eidw	115	33	S	S	NNP
cord-345088-krb1eidw	115	34	protein	protein	NN
cord-345088-krb1eidw	115	35	may	may	MD
cord-345088-krb1eidw	115	36	be	be	VB
cord-345088-krb1eidw	115	37	responsible	responsible	JJ
cord-345088-krb1eidw	115	38	for	for	IN
cord-345088-krb1eidw	115	39	the	the	DT
cord-345088-krb1eidw	115	40	temperature	temperature	NN
cord-345088-krb1eidw	115	41	sensitivity	sensitivity	NN
cord-345088-krb1eidw	115	42	of	of	IN
cord-345088-krb1eidw	115	43	the	the	DT
cord-345088-krb1eidw	115	44	mutant	mutant	JJ
cord-345088-krb1eidw	115	45	virus	virus	NN
cord-345088-krb1eidw	115	46	.	.	.
cord-345088-krb1eidw	116	1	This	this	DT
cord-345088-krb1eidw	116	2	possibility	possibility	NN
cord-345088-krb1eidw	116	3	was	be	VBD
cord-345088-krb1eidw	116	4	studied	study	VBN
cord-345088-krb1eidw	116	5	by	by	IN
cord-345088-krb1eidw	116	6	biochemical	biochemical	JJ
cord-345088-krb1eidw	116	7	and	and	CC
cord-345088-krb1eidw	116	8	functional	functional	JJ
cord-345088-krb1eidw	116	9	characterization	characterization	NN
cord-345088-krb1eidw	116	10	of	of	IN
cord-345088-krb1eidw	116	11	the	the	DT
cord-345088-krb1eidw	116	12	S	S	NNP
cord-345088-krb1eidw	116	13	protein	protein	NN
cord-345088-krb1eidw	116	14	from	from	IN
cord-345088-krb1eidw	116	15	the	the	DT
cord-345088-krb1eidw	116	16	revertant	revertant	NN
cord-345088-krb1eidw	116	17	and	and	CC
cord-345088-krb1eidw	116	18	a	a	DT
cord-345088-krb1eidw	116	19	ts	ts	NNP
cord-345088-krb1eidw	116	20	mutant	mutant	NN
cord-345088-krb1eidw	116	21	.	.	.
cord-345088-krb1eidw	117	1	The	the	DT
cord-345088-krb1eidw	117	2	S	S	NNP
cord-345088-krb1eidw	117	3	gene	gene	NN
cord-345088-krb1eidw	117	4	of	of	IN
cord-345088-krb1eidw	117	5	rev-1	rev-1	NNP
cord-345088-krb1eidw	117	6	and	and	CC
cord-345088-krb1eidw	117	7	ts291602	ts291602	NNP
cord-345088-krb1eidw	117	8	was	be	VBD
cord-345088-krb1eidw	117	9	cloned	clone	VBN
cord-345088-krb1eidw	117	10	under	under	IN
cord-345088-krb1eidw	117	11	the	the	DT
cord-345088-krb1eidw	117	12	control	control	NN
cord-345088-krb1eidw	117	13	of	of	IN
cord-345088-krb1eidw	117	14	a	a	DT
cord-345088-krb1eidw	117	15	T7	T7	NNP
cord-345088-krb1eidw	117	16	promoter	promoter	NN
cord-345088-krb1eidw	117	17	to	to	TO
cord-345088-krb1eidw	117	18	investigate	investigate	VB
cord-345088-krb1eidw	117	19	whether	whether	IN
cord-345088-krb1eidw	117	20	membranefusion	membranefusion	NN
cord-345088-krb1eidw	117	21	activity	activity	NN
cord-345088-krb1eidw	117	22	was	be	VBD
cord-345088-krb1eidw	117	23	affected	affect	VBN
cord-345088-krb1eidw	117	24	by	by	IN
cord-345088-krb1eidw	117	25	the	the	DT
cord-345088-krb1eidw	117	26	mutations	mutation	NNS
cord-345088-krb1eidw	117	27	in	in	IN
cord-345088-krb1eidw	117	28	the	the	DT
cord-345088-krb1eidw	117	29	S	S	NNP
cord-345088-krb1eidw	117	30	protein	protein	NN
cord-345088-krb1eidw	117	31	.	.	.
cord-345088-krb1eidw	118	1	Cells	cell	NNS
cord-345088-krb1eidw	118	2	were	be	VBD
cord-345088-krb1eidw	118	3	infected	infect	VBN
cord-345088-krb1eidw	118	4	with	with	IN
cord-345088-krb1eidw	118	5	recombinant	recombinant	JJ
cord-345088-krb1eidw	118	6	vaccinia	vaccinia	NN
cord-345088-krb1eidw	118	7	/	/	SYM
cord-345088-krb1eidw	118	8	T7	t7	NN
cord-345088-krb1eidw	118	9	virus	virus	NN
cord-345088-krb1eidw	118	10	and	and	CC
cord-345088-krb1eidw	118	11	were	be	VBD
cord-345088-krb1eidw	118	12	transfected	transfecte	VBN
cord-345088-krb1eidw	118	13	with	with	IN
cord-345088-krb1eidw	118	14	plasmids	plasmid	NNS
cord-345088-krb1eidw	118	15	containing	contain	VBG
cord-345088-krb1eidw	118	16	the	the	DT
cord-345088-krb1eidw	118	17	S	S	NNP
cord-345088-krb1eidw	118	18	gene	gene	NN
cord-345088-krb1eidw	118	19	from	from	IN
cord-345088-krb1eidw	118	20	either	either	CC
cord-345088-krb1eidw	118	21	ts291602	ts291602	NNP
cord-345088-krb1eidw	118	22	or	or	CC
cord-345088-krb1eidw	118	23	rev-1	rev-1	NNP
cord-345088-krb1eidw	118	24	.	.	.
cord-345088-krb1eidw	119	1	The	the	DT
cord-345088-krb1eidw	119	2	expression	expression	NN
cord-345088-krb1eidw	119	3	of	of	IN
cord-345088-krb1eidw	119	4	the	the	DT
cord-345088-krb1eidw	119	5	S	S	NNP
cord-345088-krb1eidw	119	6	proteins	protein	NNS
cord-345088-krb1eidw	119	7	was	be	VBD
cord-345088-krb1eidw	119	8	analyzed	analyze	VBN
cord-345088-krb1eidw	119	9	by	by	IN
cord-345088-krb1eidw	119	10	Western	western	JJ
cord-345088-krb1eidw	119	11	blotting	blotting	NN
cord-345088-krb1eidw	119	12	using	use	VBG
cord-345088-krb1eidw	119	13	anti	anti	JJ
cord-345088-krb1eidw	119	14	-	-	JJ
cord-345088-krb1eidw	119	15	IBV	IBV	NNP
cord-345088-krb1eidw	119	16	antibodies	antibody	NNS
cord-345088-krb1eidw	119	17	.	.	.
cord-345088-krb1eidw	120	1	As	as	IN
cord-345088-krb1eidw	120	2	shown	show	VBN
cord-345088-krb1eidw	120	3	in	in	IN
cord-345088-krb1eidw	120	4	Fig	Fig	NNP
cord-345088-krb1eidw	120	5	.	.	.
cord-345088-krb1eidw	121	1	2a	2a	LS
cord-345088-krb1eidw	121	2	,	,	,
cord-345088-krb1eidw	121	3	the	the	DT
cord-345088-krb1eidw	121	4	S	S	NNP
cord-345088-krb1eidw	121	5	protein	protein	NN
cord-345088-krb1eidw	121	6	of	of	IN
cord-345088-krb1eidw	121	7	both	both	DT
cord-345088-krb1eidw	121	8	revertant	revertant	NN
cord-345088-krb1eidw	121	9	and	and	CC
cord-345088-krb1eidw	121	10	ts	ts	NNP
cord-345088-krb1eidw	121	11	mutant	mutant	NNP
cord-345088-krb1eidw	121	12	was	be	VBD
cord-345088-krb1eidw	121	13	expressed	express	VBN
cord-345088-krb1eidw	121	14	at	at	IN
cord-345088-krb1eidw	121	15	32	32	CD
cord-345088-krb1eidw	121	16	and	and	CC
cord-345088-krb1eidw	121	17	40	40	CD
cord-345088-krb1eidw	121	18	jC	jC	NNP
cord-345088-krb1eidw	121	19	(	(	-LRB-
cord-345088-krb1eidw	121	20	lanes	lane	NNS
cord-345088-krb1eidw	121	21	3	3	CD
cord-345088-krb1eidw	121	22	,	,	,
cord-345088-krb1eidw	121	23	4	4	CD
cord-345088-krb1eidw	121	24	,	,	,
cord-345088-krb1eidw	121	25	5	5	CD
cord-345088-krb1eidw	121	26	,	,	,
cord-345088-krb1eidw	121	27	and	and	CC
cord-345088-krb1eidw	121	28	6	6	CD
cord-345088-krb1eidw	121	29	)	)	-RRB-
cord-345088-krb1eidw	121	30	,	,	,
cord-345088-krb1eidw	121	31	though	though	IN
cord-345088-krb1eidw	121	32	the	the	DT
cord-345088-krb1eidw	121	33	S	S	NNP
cord-345088-krb1eidw	121	34	protein	protein	NN
cord-345088-krb1eidw	121	35	of	of	IN
cord-345088-krb1eidw	121	36	ts291602	ts291602	NNP
cord-345088-krb1eidw	121	37	was	be	VBD
cord-345088-krb1eidw	121	38	expressed	express	VBN
cord-345088-krb1eidw	121	39	at	at	IN
cord-345088-krb1eidw	121	40	a	a	DT
cord-345088-krb1eidw	121	41	relatively	relatively	RB
cord-345088-krb1eidw	121	42	lower	low	JJR
cord-345088-krb1eidw	121	43	level	level	NN
cord-345088-krb1eidw	121	44	than	than	IN
cord-345088-krb1eidw	121	45	the	the	DT
cord-345088-krb1eidw	121	46	rev-1	rev-1	NNP
cord-345088-krb1eidw	121	47	at	at	IN
cord-345088-krb1eidw	121	48	40	40	CD
cord-345088-krb1eidw	121	49	jC	jC	NNS
cord-345088-krb1eidw	122	1	(	(	-LRB-
cord-345088-krb1eidw	122	2	compare	compare	VB
cord-345088-krb1eidw	122	3	lanes	lane	NNS
cord-345088-krb1eidw	122	4	4	4	CD
cord-345088-krb1eidw	122	5	and	and	CC
cord-345088-krb1eidw	122	6	6	6	CD
cord-345088-krb1eidw	122	7	)	)	-RRB-
cord-345088-krb1eidw	122	8	.	.	.
cord-345088-krb1eidw	123	1	The	the	DT
cord-345088-krb1eidw	123	2	fusion	fusion	NN
cord-345088-krb1eidw	123	3	activity	activity	NN
cord-345088-krb1eidw	123	4	of	of	IN
cord-345088-krb1eidw	123	5	the	the	DT
cord-345088-krb1eidw	123	6	S	S	NNP
cord-345088-krb1eidw	123	7	protein	protein	NN
cord-345088-krb1eidw	123	8	derived	derive	VBN
cord-345088-krb1eidw	123	9	from	from	IN
cord-345088-krb1eidw	123	10	the	the	DT
cord-345088-krb1eidw	123	11	revertant	revertant	NN
cord-345088-krb1eidw	123	12	and	and	CC
cord-345088-krb1eidw	123	13	ts291602	ts291602	NNP
cord-345088-krb1eidw	123	14	,	,	,
cord-345088-krb1eidw	123	15	respectively	respectively	RB
cord-345088-krb1eidw	123	16	,	,	,
cord-345088-krb1eidw	123	17	was	be	VBD
cord-345088-krb1eidw	123	18	then	then	RB
cord-345088-krb1eidw	123	19	examined	examine	VBN
cord-345088-krb1eidw	123	20	.	.	.
cord-345088-krb1eidw	124	1	As	as	IN
cord-345088-krb1eidw	124	2	shown	show	VBN
cord-345088-krb1eidw	124	3	in	in	IN
cord-345088-krb1eidw	124	4	Fig	Fig	NNP
cord-345088-krb1eidw	124	5	.	.	.
cord-345088-krb1eidw	125	1	2b	2b	NNP
cord-345088-krb1eidw	125	2	,	,	,
cord-345088-krb1eidw	125	3	membrane	membrane	NN
cord-345088-krb1eidw	125	4	fusion	fusion	NN
cord-345088-krb1eidw	125	5	was	be	VBD
cord-345088-krb1eidw	125	6	observed	observe	VBN
cord-345088-krb1eidw	125	7	in	in	IN
cord-345088-krb1eidw	125	8	cells	cell	NNS
cord-345088-krb1eidw	125	9	expressing	express	VBG
cord-345088-krb1eidw	125	10	the	the	DT
cord-345088-krb1eidw	125	11	S	S	NNP
cord-345088-krb1eidw	125	12	gene	gene	NN
cord-345088-krb1eidw	125	13	of	of	IN
cord-345088-krb1eidw	125	14	ts291602	ts291602	NNP
cord-345088-krb1eidw	125	15	at	at	IN
cord-345088-krb1eidw	125	16	32	32	CD
cord-345088-krb1eidw	125	17	jC	jC	NNP
cord-345088-krb1eidw	125	18	(	(	-LRB-
cord-345088-krb1eidw	125	19	panel	panel	NN
cord-345088-krb1eidw	125	20	C	C	NNP
cord-345088-krb1eidw	125	21	)	)	-RRB-
cord-345088-krb1eidw	125	22	but	but	CC
cord-345088-krb1eidw	125	23	not	not	RB
cord-345088-krb1eidw	125	24	at	at	IN
cord-345088-krb1eidw	125	25	40	40	CD
cord-345088-krb1eidw	125	26	jC	jC	NNP
cord-345088-krb1eidw	125	27	(	(	-LRB-
cord-345088-krb1eidw	125	28	panel	panel	NN
cord-345088-krb1eidw	125	29	F	F	NNP
cord-345088-krb1eidw	125	30	)	)	-RRB-
cord-345088-krb1eidw	125	31	,	,	,
cord-345088-krb1eidw	125	32	at	at	IN
cord-345088-krb1eidw	125	33	48	48	CD
cord-345088-krb1eidw	125	34	h	h	NN
cord-345088-krb1eidw	125	35	posttransfection	posttransfection	NN
cord-345088-krb1eidw	125	36	.	.	.
cord-345088-krb1eidw	126	1	In	in	IN
cord-345088-krb1eidw	126	2	contrast	contrast	NN
cord-345088-krb1eidw	126	3	,	,	,
cord-345088-krb1eidw	126	4	membrane	membrane	NN
cord-345088-krb1eidw	126	5	fusion	fusion	NN
cord-345088-krb1eidw	126	6	was	be	VBD
cord-345088-krb1eidw	126	7	observed	observe	VBN
cord-345088-krb1eidw	126	8	in	in	IN
cord-345088-krb1eidw	126	9	cells	cell	NNS
cord-345088-krb1eidw	126	10	expressing	express	VBG
cord-345088-krb1eidw	126	11	the	the	DT
cord-345088-krb1eidw	126	12	S	S	NNP
cord-345088-krb1eidw	126	13	gene	gene	NN
cord-345088-krb1eidw	126	14	of	of	IN
cord-345088-krb1eidw	126	15	revertant	revertant	NN
cord-345088-krb1eidw	126	16	at	at	IN
cord-345088-krb1eidw	126	17	both	both	DT
cord-345088-krb1eidw	126	18	32	32	CD
cord-345088-krb1eidw	126	19	and	and	CC
cord-345088-krb1eidw	126	20	40	40	CD
cord-345088-krb1eidw	126	21	jC	jC	NNS
cord-345088-krb1eidw	127	1	(	(	-LRB-
cord-345088-krb1eidw	127	2	panels	panel	NNS
cord-345088-krb1eidw	127	3	B	b	NN
cord-345088-krb1eidw	127	4	and	and	CC
cord-345088-krb1eidw	127	5	E	E	NNP
cord-345088-krb1eidw	127	6	)	)	-RRB-
cord-345088-krb1eidw	127	7	.	.	.
cord-345088-krb1eidw	128	1	Interestingly	interestingly	RB
cord-345088-krb1eidw	128	2	,	,	,
cord-345088-krb1eidw	128	3	membrane	membrane	NN
cord-345088-krb1eidw	128	4	fusion	fusion	NN
cord-345088-krb1eidw	128	5	appeared	appear	VBD
cord-345088-krb1eidw	128	6	at	at	IN
cord-345088-krb1eidw	128	7	72	72	CD
cord-345088-krb1eidw	128	8	h	h	NN
cord-345088-krb1eidw	128	9	posttransfection	posttransfection	NN
cord-345088-krb1eidw	128	10	,	,	,
cord-345088-krb1eidw	128	11	after	after	IN
cord-345088-krb1eidw	128	12	the	the	DT
cord-345088-krb1eidw	128	13	cells	cell	NNS
cord-345088-krb1eidw	128	14	expressing	express	VBG
cord-345088-krb1eidw	128	15	the	the	DT
cord-345088-krb1eidw	128	16	S	S	NNP
cord-345088-krb1eidw	128	17	gene	gene	NN
cord-345088-krb1eidw	128	18	of	of	IN
cord-345088-krb1eidw	128	19	ts291602	ts291602	NNP
cord-345088-krb1eidw	128	20	were	be	VBD
cord-345088-krb1eidw	128	21	shifted	shift	VBN
cord-345088-krb1eidw	128	22	from	from	IN
cord-345088-krb1eidw	128	23	40	40	CD
cord-345088-krb1eidw	128	24	to	to	TO
cord-345088-krb1eidw	128	25	32	32	CD
cord-345088-krb1eidw	128	26	jC	jC	NNS
cord-345088-krb1eidw	128	27	at	at	IN
cord-345088-krb1eidw	128	28	48	48	CD
cord-345088-krb1eidw	128	29	h	h	NN
cord-345088-krb1eidw	128	30	posttransfection	posttransfection	NN
cord-345088-krb1eidw	128	31	(	(	-LRB-
cord-345088-krb1eidw	128	32	panel	panel	NN
cord-345088-krb1eidw	128	33	I	I	NNP
cord-345088-krb1eidw	128	34	)	)	-RRB-
cord-345088-krb1eidw	128	35	.	.	.
cord-345088-krb1eidw	129	1	The	the	DT
cord-345088-krb1eidw	129	2	recombinant	recombinant	JJ
cord-345088-krb1eidw	129	3	S	S	NNP
cord-345088-krb1eidw	129	4	genes	gene	NNS
cord-345088-krb1eidw	129	5	of	of	IN
cord-345088-krb1eidw	129	6	the	the	DT
cord-345088-krb1eidw	129	7	ts	ts	NNP
cord-345088-krb1eidw	129	8	mutant	mutant	NN
cord-345088-krb1eidw	129	9	and	and	CC
cord-345088-krb1eidw	129	10	revertant	revertant	NNP
cord-345088-krb1eidw	129	11	were	be	VBD
cord-345088-krb1eidw	129	12	transfected	transfecte	VBN
cord-345088-krb1eidw	129	13	into	into	IN
cord-345088-krb1eidw	129	14	cells	cell	NNS
cord-345088-krb1eidw	129	15	in	in	IN
cord-345088-krb1eidw	129	16	35	35	CD
cord-345088-krb1eidw	129	17	mm	mm	NN
cord-345088-krb1eidw	129	18	dishes	dish	NNS
cord-345088-krb1eidw	129	19	at	at	IN
cord-345088-krb1eidw	129	20	32	32	CD
cord-345088-krb1eidw	129	21	jC	jC	NNS
cord-345088-krb1eidw	129	22	and	and	CC
cord-345088-krb1eidw	129	23	one	one	CD
cord-345088-krb1eidw	129	24	of	of	IN
cord-345088-krb1eidw	129	25	the	the	DT
cord-345088-krb1eidw	129	26	duplicates	duplicate	NNS
cord-345088-krb1eidw	129	27	was	be	VBD
cord-345088-krb1eidw	129	28	shifted	shift	VBN
cord-345088-krb1eidw	129	29	to	to	IN
cord-345088-krb1eidw	129	30	40	40	CD
cord-345088-krb1eidw	129	31	jC	jC	NNS
cord-345088-krb1eidw	129	32	at	at	IN
cord-345088-krb1eidw	129	33	4	4	CD
cord-345088-krb1eidw	129	34	h	h	NN
cord-345088-krb1eidw	129	35	posttransfection	posttransfection	NN
cord-345088-krb1eidw	129	36	.	.	.
cord-345088-krb1eidw	130	1	Radiolabeled	radiolabele	VBN
cord-345088-krb1eidw	130	2	proteins	protein	NNS
cord-345088-krb1eidw	130	3	were	be	VBD
cord-345088-krb1eidw	130	4	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	130	5	and	and	CC
cord-345088-krb1eidw	130	6	treated	treat	VBN
cord-345088-krb1eidw	130	7	with	with	IN
cord-345088-krb1eidw	130	8	Endo	Endo	NNP
cord-345088-krb1eidw	130	9	-	-	HYPH
cord-345088-krb1eidw	130	10	H	H	NNP
cord-345088-krb1eidw	130	11	at	at	IN
cord-345088-krb1eidw	130	12	37	37	CD
cord-345088-krb1eidw	130	13	jC.	jc.	NN
cord-345088-krb1eidw	131	1	As	as	IN
cord-345088-krb1eidw	131	2	shown	show	VBN
cord-345088-krb1eidw	131	3	in	in	IN
cord-345088-krb1eidw	131	4	Fig	Fig	NNP
cord-345088-krb1eidw	131	5	.	.	.
cord-345088-krb1eidw	132	1	3a	3a	NNP
cord-345088-krb1eidw	132	2	,	,	,
cord-345088-krb1eidw	132	3	the	the	DT
cord-345088-krb1eidw	132	4	ts	ts	NNP
cord-345088-krb1eidw	132	5	mutant	mutant	NN
cord-345088-krb1eidw	132	6	S	S	NNP
cord-345088-krb1eidw	132	7	protein	protein	NN
cord-345088-krb1eidw	132	8	,	,	,
cord-345088-krb1eidw	132	9	synthesized	synthesize	VBN
cord-345088-krb1eidw	132	10	at	at	IN
cord-345088-krb1eidw	132	11	40	40	CD
cord-345088-krb1eidw	132	12	jC	jC	NNS
cord-345088-krb1eidw	132	13	,	,	,
cord-345088-krb1eidw	132	14	was	be	VBD
cord-345088-krb1eidw	132	15	sensitive	sensitive	JJ
cord-345088-krb1eidw	132	16	to	to	IN
cord-345088-krb1eidw	132	17	Endo	endo	NN
cord-345088-krb1eidw	132	18	-	-	HYPH
cord-345088-krb1eidw	132	19	H	H	NNP
cord-345088-krb1eidw	132	20	digestion	digestion	NN
cord-345088-krb1eidw	132	21	,	,	,
cord-345088-krb1eidw	132	22	as	as	IN
cord-345088-krb1eidw	132	23	no	no	DT
cord-345088-krb1eidw	132	24	180-kDa	180-kda	CD
cord-345088-krb1eidw	132	25	form	form	NN
cord-345088-krb1eidw	132	26	was	be	VBD
cord-345088-krb1eidw	132	27	observed	observe	VBN
cord-345088-krb1eidw	132	28	(	(	-LRB-
cord-345088-krb1eidw	132	29	lane	lane	NN
cord-345088-krb1eidw	132	30	12	12	CD
cord-345088-krb1eidw	132	31	)	)	-RRB-
cord-345088-krb1eidw	132	32	.	.	.
cord-345088-krb1eidw	133	1	In	in	IN
cord-345088-krb1eidw	133	2	contrast	contrast	NN
cord-345088-krb1eidw	133	3	,	,	,
cord-345088-krb1eidw	133	4	a	a	DT
cord-345088-krb1eidw	133	5	portion	portion	NN
cord-345088-krb1eidw	133	6	of	of	IN
cord-345088-krb1eidw	133	7	the	the	DT
cord-345088-krb1eidw	133	8	ts	ts	NNP
cord-345088-krb1eidw	133	9	mutant	mutant	NN
cord-345088-krb1eidw	133	10	S	S	NNP
cord-345088-krb1eidw	133	11	protein	protein	NN
cord-345088-krb1eidw	133	12	,	,	,
cord-345088-krb1eidw	133	13	synthesized	synthesize	VBN
cord-345088-krb1eidw	133	14	at	at	IN
cord-345088-krb1eidw	133	15	32	32	CD
cord-345088-krb1eidw	133	16	jC	jC	NNS
cord-345088-krb1eidw	134	1	(	(	-LRB-
cord-345088-krb1eidw	134	2	lanes	lane	NNS
cord-345088-krb1eidw	134	3	10	10	CD
cord-345088-krb1eidw	134	4	)	)	-RRB-
cord-345088-krb1eidw	134	5	,	,	,
cord-345088-krb1eidw	134	6	was	be	VBD
cord-345088-krb1eidw	134	7	resistant	resistant	JJ
cord-345088-krb1eidw	134	8	to	to	IN
cord-345088-krb1eidw	134	9	Endo	endo	NN
cord-345088-krb1eidw	134	10	-	-	HYPH
cord-345088-krb1eidw	134	11	H	H	NNP
cord-345088-krb1eidw	134	12	digestion	digestion	NN
cord-345088-krb1eidw	134	13	.	.	.
cord-345088-krb1eidw	135	1	The	the	DT
cord-345088-krb1eidw	135	2	revertant	revertant	NN
cord-345088-krb1eidw	135	3	S	s	NN
cord-345088-krb1eidw	135	4	protein	protein	NN
cord-345088-krb1eidw	135	5	,	,	,
cord-345088-krb1eidw	135	6	synthesized	synthesize	VBN
cord-345088-krb1eidw	135	7	at	at	IN
cord-345088-krb1eidw	135	8	both	both	DT
cord-345088-krb1eidw	135	9	32	32	CD
cord-345088-krb1eidw	135	10	and	and	CC
cord-345088-krb1eidw	135	11	40	40	CD
cord-345088-krb1eidw	135	12	jC	jC	NNS
cord-345088-krb1eidw	135	13	,	,	,
cord-345088-krb1eidw	135	14	was	be	VBD
cord-345088-krb1eidw	135	15	also	also	RB
cord-345088-krb1eidw	135	16	resistant	resistant	JJ
cord-345088-krb1eidw	135	17	to	to	IN
cord-345088-krb1eidw	135	18	Endo	endo	NN
cord-345088-krb1eidw	135	19	-	-	HYPH
cord-345088-krb1eidw	135	20	H	H	NNP
cord-345088-krb1eidw	135	21	digestion	digestion	NN
cord-345088-krb1eidw	135	22	(	(	-LRB-
cord-345088-krb1eidw	135	23	lanes	lane	NNS
cord-345088-krb1eidw	135	24	6	6	CD
cord-345088-krb1eidw	135	25	and	and	CC
cord-345088-krb1eidw	135	26	8)	8)	NN
cord-345088-krb1eidw	135	27	.	.	.
cord-345088-krb1eidw	136	1	These	these	DT
cord-345088-krb1eidw	136	2	a	a	DT
cord-345088-krb1eidw	136	3	b	b	NN
cord-345088-krb1eidw	136	4	Fig	Fig	NNP
cord-345088-krb1eidw	136	5	.	.	.
cord-345088-krb1eidw	137	1	1	1	LS
cord-345088-krb1eidw	137	2	.	.	.
cord-345088-krb1eidw	138	1	Analysis	analysis	NN
cord-345088-krb1eidw	138	2	of	of	IN
cord-345088-krb1eidw	138	3	the	the	DT
cord-345088-krb1eidw	138	4	expression	expression	NN
cord-345088-krb1eidw	138	5	of	of	IN
cord-345088-krb1eidw	138	6	the	the	DT
cord-345088-krb1eidw	138	7	S	S	NNP
cord-345088-krb1eidw	138	8	protein	protein	NN
cord-345088-krb1eidw	138	9	from	from	IN
cord-345088-krb1eidw	138	10	wt	wt	NN
cord-345088-krb1eidw	138	11	(	(	-LRB-
cord-345088-krb1eidw	138	12	wt6501	wt6501	NN
cord-345088-krb1eidw	138	13	)	)	-RRB-
cord-345088-krb1eidw	138	14	,	,	,
cord-345088-krb1eidw	138	15	ts	ts	NNP
cord-345088-krb1eidw	138	16	mutants	mutant	NNS
cord-345088-krb1eidw	138	17	(	(	-LRB-
cord-345088-krb1eidw	138	18	ts291602	ts291602	NN
cord-345088-krb1eidw	138	19	and	and	CC
cord-345088-krb1eidw	138	20	282902	282902	CD
cord-345088-krb1eidw	138	21	)	)	-RRB-
cord-345088-krb1eidw	138	22	and	and	CC
cord-345088-krb1eidw	138	23	revertant	revertant	NNP
cord-345088-krb1eidw	138	24	(	(	-LRB-
cord-345088-krb1eidw	138	25	rev-1	rev-1	NNP
cord-345088-krb1eidw	138	26	)	)	-RRB-
cord-345088-krb1eidw	138	27	viruses	virus	NNS
cord-345088-krb1eidw	138	28	.	.	.
cord-345088-krb1eidw	139	1	(	(	-LRB-
cord-345088-krb1eidw	139	2	a	a	LS
cord-345088-krb1eidw	139	3	)	)	-RRB-
cord-345088-krb1eidw	139	4	Cells	cell	NNS
cord-345088-krb1eidw	139	5	were	be	VBD
cord-345088-krb1eidw	139	6	infected	infect	VBN
cord-345088-krb1eidw	139	7	with	with	IN
cord-345088-krb1eidw	139	8	each	each	DT
cord-345088-krb1eidw	139	9	virus	virus	NN
cord-345088-krb1eidw	139	10	at	at	IN
cord-345088-krb1eidw	139	11	32	32	CD
cord-345088-krb1eidw	139	12	jC	jC	NNP
cord-345088-krb1eidw	139	13	and	and	CC
cord-345088-krb1eidw	139	14	40	40	CD
cord-345088-krb1eidw	139	15	jC	jC	NNS
cord-345088-krb1eidw	139	16	as	as	IN
cord-345088-krb1eidw	139	17	indicated	indicate	VBN
cord-345088-krb1eidw	139	18	on	on	IN
cord-345088-krb1eidw	139	19	the	the	DT
cord-345088-krb1eidw	139	20	top	top	NN
cord-345088-krb1eidw	139	21	for	for	IN
cord-345088-krb1eidw	139	22	1	1	CD
cord-345088-krb1eidw	139	23	h	h	NN
cord-345088-krb1eidw	139	24	and	and	CC
cord-345088-krb1eidw	139	25	were	be	VBD
cord-345088-krb1eidw	139	26	maintained	maintain	VBN
cord-345088-krb1eidw	139	27	at	at	IN
cord-345088-krb1eidw	139	28	the	the	DT
cord-345088-krb1eidw	139	29	same	same	JJ
cord-345088-krb1eidw	139	30	temperatures	temperature	NNS
cord-345088-krb1eidw	139	31	.	.	.
cord-345088-krb1eidw	140	1	(	(	-LRB-
cord-345088-krb1eidw	140	2	b	b	NN
cord-345088-krb1eidw	140	3	)	)	-RRB-
cord-345088-krb1eidw	141	1	Two	two	CD
cord-345088-krb1eidw	141	2	dishes	dish	NNS
cord-345088-krb1eidw	141	3	of	of	IN
cord-345088-krb1eidw	141	4	cells	cell	NNS
cord-345088-krb1eidw	141	5	were	be	VBD
cord-345088-krb1eidw	141	6	infected	infect	VBN
cord-345088-krb1eidw	141	7	with	with	IN
cord-345088-krb1eidw	141	8	each	each	DT
cord-345088-krb1eidw	141	9	virus	virus	NN
cord-345088-krb1eidw	141	10	at	at	IN
cord-345088-krb1eidw	141	11	32	32	CD
cord-345088-krb1eidw	141	12	jC	jC	NNP
cord-345088-krb1eidw	141	13	for	for	IN
cord-345088-krb1eidw	141	14	1	1	CD
cord-345088-krb1eidw	141	15	h.	h.	NN
cord-345088-krb1eidw	141	16	One	one	CD
cord-345088-krb1eidw	141	17	of	of	IN
cord-345088-krb1eidw	141	18	the	the	DT
cord-345088-krb1eidw	141	19	duplicates	duplicate	NNS
cord-345088-krb1eidw	141	20	was	be	VBD
cord-345088-krb1eidw	141	21	maintained	maintain	VBN
cord-345088-krb1eidw	141	22	at	at	IN
cord-345088-krb1eidw	141	23	32	32	CD
cord-345088-krb1eidw	141	24	jC	jC	NNP
cord-345088-krb1eidw	141	25	(	(	-LRB-
cord-345088-krb1eidw	141	26	lanes	lane	NNS
cord-345088-krb1eidw	141	27	1	1	CD
cord-345088-krb1eidw	141	28	,	,	,
cord-345088-krb1eidw	141	29	3	3	CD
cord-345088-krb1eidw	141	30	,	,	,
cord-345088-krb1eidw	141	31	5	5	CD
cord-345088-krb1eidw	141	32	,	,	,
cord-345088-krb1eidw	141	33	and	and	CC
cord-345088-krb1eidw	141	34	7	7	CD
cord-345088-krb1eidw	141	35	)	)	-RRB-
cord-345088-krb1eidw	141	36	and	and	CC
cord-345088-krb1eidw	141	37	the	the	DT
cord-345088-krb1eidw	141	38	other	other	JJ
cord-345088-krb1eidw	141	39	one	one	NN
cord-345088-krb1eidw	141	40	was	be	VBD
cord-345088-krb1eidw	141	41	shifted	shift	VBN
cord-345088-krb1eidw	141	42	to	to	IN
cord-345088-krb1eidw	141	43	40	40	CD
cord-345088-krb1eidw	141	44	jC	jC	NNP
cord-345088-krb1eidw	141	45	(	(	-LRB-
cord-345088-krb1eidw	141	46	lanes	lane	NNS
cord-345088-krb1eidw	141	47	2	2	CD
cord-345088-krb1eidw	141	48	,	,	,
cord-345088-krb1eidw	141	49	4	4	CD
cord-345088-krb1eidw	141	50	,	,	,
cord-345088-krb1eidw	141	51	6	6	CD
cord-345088-krb1eidw	141	52	,	,	,
cord-345088-krb1eidw	141	53	and	and	CC
cord-345088-krb1eidw	141	54	8)	8)	NN
cord-345088-krb1eidw	141	55	.	.	.
cord-345088-krb1eidw	142	1	Radiolabeled	radiolabeled	JJ
cord-345088-krb1eidw	142	2	cell	cell	NN
cord-345088-krb1eidw	142	3	lysates	lysate	NNS
cord-345088-krb1eidw	142	4	were	be	VBD
cord-345088-krb1eidw	142	5	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	142	6	with	with	IN
cord-345088-krb1eidw	142	7	anti	anti	JJ
cord-345088-krb1eidw	142	8	-	-	JJ
cord-345088-krb1eidw	142	9	IBV	IBV	NNP
cord-345088-krb1eidw	142	10	antibodies	antibody	NNS
cord-345088-krb1eidw	142	11	.	.	.
cord-345088-krb1eidw	143	1	The	the	DT
cord-345088-krb1eidw	143	2	proteins	protein	NNS
cord-345088-krb1eidw	143	3	were	be	VBD
cord-345088-krb1eidw	143	4	separated	separate	VBN
cord-345088-krb1eidw	143	5	on	on	IN
cord-345088-krb1eidw	143	6	12.5	12.5	CD
cord-345088-krb1eidw	143	7	%	%	NN
cord-345088-krb1eidw	143	8	polyacrylamide	polyacrylamide	NN
cord-345088-krb1eidw	143	9	gels	gel	NNS
cord-345088-krb1eidw	143	10	and	and	CC
cord-345088-krb1eidw	143	11	detected	detect	VBN
cord-345088-krb1eidw	143	12	by	by	IN
cord-345088-krb1eidw	143	13	autoradiography	autoradiography	NNP
cord-345088-krb1eidw	143	14	.	.	.
cord-345088-krb1eidw	144	1	Numbers	number	NNS
cord-345088-krb1eidw	144	2	on	on	IN
cord-345088-krb1eidw	144	3	the	the	DT
cord-345088-krb1eidw	144	4	left	left	JJ
cord-345088-krb1eidw	144	5	indicate	indicate	VBP
cord-345088-krb1eidw	144	6	molecular	molecular	JJ
cord-345088-krb1eidw	144	7	mass	mass	NN
cord-345088-krb1eidw	144	8	in	in	IN
cord-345088-krb1eidw	144	9	kilodalton	kilodalton	NNP
cord-345088-krb1eidw	144	10	and	and	CC
cord-345088-krb1eidw	144	11	the	the	DT
cord-345088-krb1eidw	144	12	position	position	NN
cord-345088-krb1eidw	144	13	of	of	IN
cord-345088-krb1eidw	144	14	the	the	DT
cord-345088-krb1eidw	144	15	S	S	NNP
cord-345088-krb1eidw	144	16	protein	protein	NN
cord-345088-krb1eidw	144	17	is	be	VBZ
cord-345088-krb1eidw	144	18	indicated	indicate	VBN
cord-345088-krb1eidw	144	19	on	on	IN
cord-345088-krb1eidw	144	20	the	the	DT
cord-345088-krb1eidw	144	21	right	right	NN
cord-345088-krb1eidw	144	22	.	.	.
cord-345088-krb1eidw	145	1	results	result	NNS
cord-345088-krb1eidw	145	2	indicate	indicate	VBP
cord-345088-krb1eidw	145	3	that	that	IN
cord-345088-krb1eidw	145	4	the	the	DT
cord-345088-krb1eidw	145	5	ts	ts	NNP
cord-345088-krb1eidw	145	6	mutant	mutant	NN
cord-345088-krb1eidw	145	7	S	S	NNP
cord-345088-krb1eidw	145	8	protein	protein	NN
cord-345088-krb1eidw	145	9	synthesized	synthesize	VBN
cord-345088-krb1eidw	145	10	at	at	IN
cord-345088-krb1eidw	145	11	40	40	CD
cord-345088-krb1eidw	145	12	jC	jC	NNP
cord-345088-krb1eidw	145	13	was	be	VBD
cord-345088-krb1eidw	145	14	not	not	RB
cord-345088-krb1eidw	145	15	a	a	DT
cord-345088-krb1eidw	145	16	mature	mature	JJ
cord-345088-krb1eidw	145	17	form	form	NN
cord-345088-krb1eidw	145	18	of	of	IN
cord-345088-krb1eidw	145	19	the	the	DT
cord-345088-krb1eidw	145	20	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	145	21	.	.	.
cord-345088-krb1eidw	146	1	In	in	IN
cord-345088-krb1eidw	146	2	addition	addition	NN
cord-345088-krb1eidw	146	3	,	,	,
cord-345088-krb1eidw	146	4	a	a	DT
cord-345088-krb1eidw	146	5	novel	novel	JJ
cord-345088-krb1eidw	146	6	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	146	7	form	form	NN
cord-345088-krb1eidw	146	8	of	of	IN
cord-345088-krb1eidw	146	9	the	the	DT
cord-345088-krb1eidw	146	10	S	S	NNP
cord-345088-krb1eidw	146	11	protein	protein	NN
cord-345088-krb1eidw	146	12	,	,	,
cord-345088-krb1eidw	146	13	migrating	migrate	VBG
cord-345088-krb1eidw	146	14	between	between	IN
cord-345088-krb1eidw	146	15	the	the	DT
cord-345088-krb1eidw	146	16	Endo	Endo	NNP
cord-345088-krb1eidw	146	17	-	-	HYPH
cord-345088-krb1eidw	146	18	H	H	NNP
cord-345088-krb1eidw	146	19	treated	treat	VBD
cord-345088-krb1eidw	146	20	130-kDa	130-kda	CD
cord-345088-krb1eidw	146	21	and	and	CC
cord-345088-krb1eidw	146	22	the	the	DT
cord-345088-krb1eidw	146	23	matured	matured	JJ
cord-345088-krb1eidw	146	24	180-kDa	180-kda	CD
cord-345088-krb1eidw	146	25	forms	form	NNS
cord-345088-krb1eidw	146	26	,	,	,
cord-345088-krb1eidw	146	27	was	be	VBD
cord-345088-krb1eidw	146	28	observed	observe	VBN
cord-345088-krb1eidw	146	29	.	.	.
cord-345088-krb1eidw	147	1	It	-PRON-	PRP
cord-345088-krb1eidw	147	2	might	may	MD
cord-345088-krb1eidw	147	3	represent	represent	VB
cord-345088-krb1eidw	147	4	trimming	trimming	NN
cord-345088-krb1eidw	147	5	of	of	IN
cord-345088-krb1eidw	147	6	an	an	DT
cord-345088-krb1eidw	147	7	initial	initial	JJ
cord-345088-krb1eidw	147	8	,	,	,
cord-345088-krb1eidw	147	9	core	core	NN
cord-345088-krb1eidw	147	10	-	-	HYPH
cord-345088-krb1eidw	147	11	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	147	12	form	form	NN
cord-345088-krb1eidw	147	13	of	of	IN
cord-345088-krb1eidw	147	14	the	the	DT
cord-345088-krb1eidw	147	15	S	S	NNP
cord-345088-krb1eidw	147	16	protein	protein	NN
cord-345088-krb1eidw	147	17	in	in	IN
cord-345088-krb1eidw	147	18	the	the	DT
cord-345088-krb1eidw	147	19	ER	ER	NNP
cord-345088-krb1eidw	147	20	and	and	CC
cord-345088-krb1eidw	147	21	the	the	DT
cord-345088-krb1eidw	147	22	cis	cis	NNP
cord-345088-krb1eidw	147	23	-	-	HYPH
cord-345088-krb1eidw	147	24	Golgi	Golgi	NNP
cord-345088-krb1eidw	147	25	.	.	.
cord-345088-krb1eidw	148	1	Interestingly	interestingly	RB
cord-345088-krb1eidw	148	2	,	,	,
cord-345088-krb1eidw	148	3	this	this	DT
cord-345088-krb1eidw	148	4	band	band	NN
cord-345088-krb1eidw	148	5	was	be	VBD
cord-345088-krb1eidw	148	6	also	also	RB
cord-345088-krb1eidw	148	7	observed	observe	VBN
cord-345088-krb1eidw	148	8	in	in	IN
cord-345088-krb1eidw	148	9	ts291602-infected	ts291602-infected	JJ
cord-345088-krb1eidw	148	10	cells	cell	NNS
cord-345088-krb1eidw	148	11	(	(	-LRB-
cord-345088-krb1eidw	148	12	Fig	Fig	NNP
cord-345088-krb1eidw	148	13	.	.	.
cord-345088-krb1eidw	148	14	3b	3b	CD
cord-345088-krb1eidw	148	15	,	,	.
cord-345088-krb1eidw	149	1	lane	lane	NNP
cord-345088-krb1eidw	149	2	 	 	_SP
cord-345088-krb1eidw	149	3	2	2	CD
cord-345088-krb1eidw	149	4	)	)	-RRB-
cord-345088-krb1eidw	149	5	,	,	,
cord-345088-krb1eidw	149	6	when	when	WRB
cord-345088-krb1eidw	149	7	the	the	DT
cord-345088-krb1eidw	149	8	incubation	incubation	NN
cord-345088-krb1eidw	149	9	time	time	NN
cord-345088-krb1eidw	149	10	at	at	IN
cord-345088-krb1eidw	149	11	32	32	CD
cord-345088-krb1eidw	149	12	jC	jC	NNP
cord-345088-krb1eidw	149	13	was	be	VBD
cord-345088-krb1eidw	149	14	extended	extend	VBN
cord-345088-krb1eidw	149	15	before	before	IN
cord-345088-krb1eidw	149	16	the	the	DT
cord-345088-krb1eidw	149	17	culture	culture	NN
cord-345088-krb1eidw	149	18	was	be	VBD
cord-345088-krb1eidw	149	19	shifted	shift	VBN
cord-345088-krb1eidw	149	20	to	to	IN
cord-345088-krb1eidw	149	21	40	40	CD
cord-345088-krb1eidw	149	22	jC.	jc.	NN
cord-345088-krb1eidw	150	1	This	this	DT
cord-345088-krb1eidw	150	2	band	band	NN
cord-345088-krb1eidw	150	3	is	be	VBZ
cord-345088-krb1eidw	150	4	much	much	RB
cord-345088-krb1eidw	150	5	stronger	strong	JJR
cord-345088-krb1eidw	150	6	than	than	IN
cord-345088-krb1eidw	150	7	the	the	DT
cord-345088-krb1eidw	150	8	180-kDa	180-kda	CD
cord-345088-krb1eidw	150	9	form	form	NN
cord-345088-krb1eidw	150	10	(	(	-LRB-
cord-345088-krb1eidw	150	11	lane	lane	NN
cord-345088-krb1eidw	150	12	2	2	CD
cord-345088-krb1eidw	150	13	)	)	-RRB-
cord-345088-krb1eidw	150	14	,	,	,
cord-345088-krb1eidw	150	15	giving	give	VBG
cord-345088-krb1eidw	150	16	direct	direct	JJ
cord-345088-krb1eidw	150	17	evidence	evidence	NN
cord-345088-krb1eidw	150	18	that	that	IN
cord-345088-krb1eidw	150	19	the	the	DT
cord-345088-krb1eidw	150	20	mutations	mutation	NNS
cord-345088-krb1eidw	150	21	in	in	IN
cord-345088-krb1eidw	150	22	the	the	DT
cord-345088-krb1eidw	150	23	S	S	NNP
cord-345088-krb1eidw	150	24	protein	protein	NN
cord-345088-krb1eidw	150	25	of	of	IN
cord-345088-krb1eidw	150	26	ts291602	ts291602	NNP
cord-345088-krb1eidw	150	27	dramatically	dramatically	RB
cord-345088-krb1eidw	150	28	hampered	hamper	VBD
cord-345088-krb1eidw	150	29	the	the	DT
cord-345088-krb1eidw	150	30	maturation	maturation	NN
cord-345088-krb1eidw	150	31	process	process	NN
cord-345088-krb1eidw	150	32	of	of	IN
cord-345088-krb1eidw	150	33	the	the	DT
cord-345088-krb1eidw	150	34	S	S	NNP
cord-345088-krb1eidw	150	35	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	150	36	.	.	.
cord-345088-krb1eidw	151	1	Compared	compare	VBN
cord-345088-krb1eidw	151	2	with	with	IN
cord-345088-krb1eidw	151	3	ts282902	ts282902	XX
cord-345088-krb1eidw	151	4	,	,	,
cord-345088-krb1eidw	151	5	the	the	DT
cord-345088-krb1eidw	151	6	S	S	NNP
cord-345088-krb1eidw	151	7	protein	protein	NN
cord-345088-krb1eidw	151	8	of	of	IN
cord-345088-krb1eidw	151	9	ts291602	ts291602	NNP
cord-345088-krb1eidw	151	10	contains	contain	VBZ
cord-345088-krb1eidw	151	11	another	another	DT
cord-345088-krb1eidw	151	12	amino	amino	NN
cord-345088-krb1eidw	151	13	acid	acid	NN
cord-345088-krb1eidw	151	14	substitution	substitution	NN
cord-345088-krb1eidw	151	15	,	,	,
cord-345088-krb1eidw	151	16	D	D	NNP
cord-345088-krb1eidw	151	17	709	709	CD
cord-345088-krb1eidw	151	18	-N	-N	NFP
cord-345088-krb1eidw	151	19	709	709	CD
cord-345088-krb1eidw	152	1	(	(	-LRB-
cord-345088-krb1eidw	152	2	Table	table	NN
cord-345088-krb1eidw	152	3	1	1	CD
cord-345088-krb1eidw	152	4	)	)	-RRB-
cord-345088-krb1eidw	152	5	,	,	,
cord-345088-krb1eidw	152	6	which	which	WDT
cord-345088-krb1eidw	152	7	is	be	VBZ
cord-345088-krb1eidw	152	8	not	not	RB
cord-345088-krb1eidw	152	9	present	present	JJ
cord-345088-krb1eidw	152	10	in	in	IN
cord-345088-krb1eidw	152	11	the	the	DT
cord-345088-krb1eidw	152	12	S	S	NNP
cord-345088-krb1eidw	152	13	protein	protein	NN
cord-345088-krb1eidw	152	14	of	of	IN
cord-345088-krb1eidw	152	15	rev-1	rev-1	NNP
cord-345088-krb1eidw	152	16	and	and	CC
cord-345088-krb1eidw	152	17	wt6501	wt6501	NNP
cord-345088-krb1eidw	152	18	.	.	.
cord-345088-krb1eidw	153	1	As	as	IN
cord-345088-krb1eidw	153	2	this	this	DT
cord-345088-krb1eidw	153	3	band	band	NN
cord-345088-krb1eidw	153	4	was	be	VBD
cord-345088-krb1eidw	153	5	not	not	RB
cord-345088-krb1eidw	153	6	observed	observe	VBN
cord-345088-krb1eidw	153	7	in	in	IN
cord-345088-krb1eidw	153	8	ts281602-infected	ts281602-infecte	VBN
cord-345088-krb1eidw	153	9	cells	cell	NNS
cord-345088-krb1eidw	153	10	(	(	-LRB-
cord-345088-krb1eidw	153	11	data	datum	NNS
cord-345088-krb1eidw	153	12	not	not	RB
cord-345088-krb1eidw	153	13	shown	show	VBN
cord-345088-krb1eidw	153	14	)	)	-RRB-
cord-345088-krb1eidw	153	15	,	,	,
cord-345088-krb1eidw	153	16	it	-PRON-	PRP
cord-345088-krb1eidw	153	17	is	be	VBZ
cord-345088-krb1eidw	153	18	not	not	RB
cord-345088-krb1eidw	153	19	clear	clear	JJ
cord-345088-krb1eidw	153	20	at	at	IN
cord-345088-krb1eidw	153	21	this	this	DT
cord-345088-krb1eidw	153	22	point	point	NN
cord-345088-krb1eidw	153	23	if	if	IN
cord-345088-krb1eidw	153	24	this	this	DT
cord-345088-krb1eidw	153	25	additional	additional	JJ
cord-345088-krb1eidw	153	26	mutation	mutation	NN
cord-345088-krb1eidw	153	27	may	may	MD
cord-345088-krb1eidw	153	28	also	also	RB
cord-345088-krb1eidw	153	29	play	play	VB
cord-345088-krb1eidw	153	30	a	a	DT
cord-345088-krb1eidw	153	31	role	role	NN
cord-345088-krb1eidw	153	32	in	in	IN
cord-345088-krb1eidw	153	33	the	the	DT
cord-345088-krb1eidw	153	34	maturation	maturation	NN
cord-345088-krb1eidw	153	35	of	of	IN
cord-345088-krb1eidw	153	36	the	the	DT
cord-345088-krb1eidw	153	37	S	S	NNP
cord-345088-krb1eidw	153	38	protein	protein	NN
cord-345088-krb1eidw	153	39	.	.	.
cord-345088-krb1eidw	154	1	It	-PRON-	PRP
cord-345088-krb1eidw	154	2	was	be	VBD
cord-345088-krb1eidw	154	3	also	also	RB
cord-345088-krb1eidw	154	4	noted	note	VBN
cord-345088-krb1eidw	154	5	that	that	IN
cord-345088-krb1eidw	154	6	,	,	,
cord-345088-krb1eidw	154	7	at	at	IN
cord-345088-krb1eidw	154	8	40	40	CD
cord-345088-krb1eidw	154	9	jC	jC	NNS
cord-345088-krb1eidw	154	10	for	for	IN
cord-345088-krb1eidw	154	11	all	all	DT
cord-345088-krb1eidw	154	12	viruses	virus	NNS
cord-345088-krb1eidw	154	13	,	,	,
cord-345088-krb1eidw	154	14	the	the	DT
cord-345088-krb1eidw	154	15	S1	S1	NNP
cord-345088-krb1eidw	154	16	and	and	CC
cord-345088-krb1eidw	154	17	S2	s2	NN
cord-345088-krb1eidw	154	18	subunits	subunit	NNS
cord-345088-krb1eidw	154	19	were	be	VBD
cord-345088-krb1eidw	154	20	hardly	hardly	RB
cord-345088-krb1eidw	154	21	detected	detect	VBN
cord-345088-krb1eidw	154	22	in	in	IN
cord-345088-krb1eidw	154	23	cell	cell	NN
cord-345088-krb1eidw	154	24	lysates	lysate	NNS
cord-345088-krb1eidw	154	25	but	but	CC
cord-345088-krb1eidw	154	26	abundant	abundant	JJ
cord-345088-krb1eidw	154	27	in	in	IN
cord-345088-krb1eidw	154	28	the	the	DT
cord-345088-krb1eidw	154	29	supernatants	supernatant	NNS
cord-345088-krb1eidw	154	30	(	(	-LRB-
cord-345088-krb1eidw	154	31	Fig	Fig	NNP
cord-345088-krb1eidw	154	32	.	.	.
cord-345088-krb1eidw	154	33	3b	3b	CD
cord-345088-krb1eidw	154	34	)	)	-RRB-
cord-345088-krb1eidw	154	35	.	.	.
cord-345088-krb1eidw	155	1	At	at	IN
cord-345088-krb1eidw	155	2	the	the	DT
cord-345088-krb1eidw	155	3	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	155	4	temperature	temperature	NN
cord-345088-krb1eidw	155	5	,	,	,
cord-345088-krb1eidw	155	6	the	the	DT
cord-345088-krb1eidw	155	7	S	S	NNP
cord-345088-krb1eidw	155	8	protein	protein	NN
cord-345088-krb1eidw	155	9	was	be	VBD
cord-345088-krb1eidw	155	10	not	not	RB
cord-345088-krb1eidw	155	11	cleaved	cleave	VBN
cord-345088-krb1eidw	155	12	at	at	RB
cord-345088-krb1eidw	155	13	all	all	DT
cord-345088-krb1eidw	155	14	(	(	-LRB-
cord-345088-krb1eidw	155	15	lanes	lane	NNS
cord-345088-krb1eidw	155	16	2	2	CD
cord-345088-krb1eidw	155	17	and	and	CC
cord-345088-krb1eidw	155	18	6	6	CD
cord-345088-krb1eidw	155	19	)	)	-RRB-
cord-345088-krb1eidw	155	20	.	.	.
cord-345088-krb1eidw	156	1	These	these	DT
cord-345088-krb1eidw	156	2	results	result	NNS
cord-345088-krb1eidw	156	3	may	may	MD
cord-345088-krb1eidw	156	4	suggest	suggest	VB
cord-345088-krb1eidw	156	5	that	that	IN
cord-345088-krb1eidw	156	6	only	only	RB
cord-345088-krb1eidw	156	7	the	the	DT
cord-345088-krb1eidw	156	8	mature	mature	JJ
cord-345088-krb1eidw	156	9	form	form	NN
cord-345088-krb1eidw	156	10	of	of	IN
cord-345088-krb1eidw	156	11	the	the	DT
cord-345088-krb1eidw	156	12	S	S	NNP
cord-345088-krb1eidw	156	13	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	156	14	could	could	MD
cord-345088-krb1eidw	156	15	be	be	VB
cord-345088-krb1eidw	156	16	cleaved	cleave	VBN
cord-345088-krb1eidw	156	17	into	into	IN
cord-345088-krb1eidw	156	18	S1	S1	NNP
cord-345088-krb1eidw	156	19	and	and	CC
cord-345088-krb1eidw	156	20	S2	S2	NNP
cord-345088-krb1eidw	156	21	and	and	CC
cord-345088-krb1eidw	156	22	Fig	Fig	NNP
cord-345088-krb1eidw	156	23	.	.	.
cord-345088-krb1eidw	157	1	2	2	LS
cord-345088-krb1eidw	157	2	.	.	.
cord-345088-krb1eidw	158	1	Membrane	membrane	NN
cord-345088-krb1eidw	158	2	fusion	fusion	NN
cord-345088-krb1eidw	158	3	activity	activity	NN
cord-345088-krb1eidw	158	4	of	of	IN
cord-345088-krb1eidw	158	5	the	the	DT
cord-345088-krb1eidw	158	6	S	S	NNP
cord-345088-krb1eidw	158	7	protein	protein	NN
cord-345088-krb1eidw	158	8	expressed	express	VBD
cord-345088-krb1eidw	158	9	from	from	IN
cord-345088-krb1eidw	158	10	wt	wt	NN
cord-345088-krb1eidw	158	11	and	and	CC
cord-345088-krb1eidw	158	12	ts291602	ts291602	NNP
cord-345088-krb1eidw	158	13	.	.	.
cord-345088-krb1eidw	159	1	(	(	-LRB-
cord-345088-krb1eidw	159	2	a	a	LS
cord-345088-krb1eidw	159	3	)	)	-RRB-
cord-345088-krb1eidw	159	4	Vero	Vero	NNP
cord-345088-krb1eidw	159	5	cells	cell	NNS
cord-345088-krb1eidw	159	6	were	be	VBD
cord-345088-krb1eidw	159	7	transfected	transfecte	VBN
cord-345088-krb1eidw	159	8	with	with	IN
cord-345088-krb1eidw	159	9	plasmids	plasmid	NNS
cord-345088-krb1eidw	159	10	without	without	IN
cord-345088-krb1eidw	159	11	insert	insert	JJ
cord-345088-krb1eidw	159	12	(	(	-LRB-
cord-345088-krb1eidw	159	13	lanes	lane	NNS
cord-345088-krb1eidw	159	14	1	1	CD
cord-345088-krb1eidw	159	15	and	and	CC
cord-345088-krb1eidw	159	16	2	2	CD
cord-345088-krb1eidw	159	17	)	)	-RRB-
cord-345088-krb1eidw	159	18	or	or	CC
cord-345088-krb1eidw	159	19	with	with	IN
cord-345088-krb1eidw	159	20	the	the	DT
cord-345088-krb1eidw	159	21	S	S	NNP
cord-345088-krb1eidw	159	22	gene	gene	NN
cord-345088-krb1eidw	159	23	from	from	IN
cord-345088-krb1eidw	159	24	the	the	DT
cord-345088-krb1eidw	159	25	wt	wt	NN
cord-345088-krb1eidw	159	26	(	(	-LRB-
cord-345088-krb1eidw	159	27	lanes	lane	NNS
cord-345088-krb1eidw	159	28	3	3	CD
cord-345088-krb1eidw	159	29	and	and	CC
cord-345088-krb1eidw	159	30	4	4	CD
cord-345088-krb1eidw	159	31	)	)	-RRB-
cord-345088-krb1eidw	159	32	and	and	CC
cord-345088-krb1eidw	159	33	ts291602	ts291602	NNP
cord-345088-krb1eidw	159	34	(	(	-LRB-
cord-345088-krb1eidw	159	35	lanes	lane	NNS
cord-345088-krb1eidw	159	36	5	5	CD
cord-345088-krb1eidw	159	37	and	and	CC
cord-345088-krb1eidw	159	38	6	6	CD
cord-345088-krb1eidw	159	39	)	)	-RRB-
cord-345088-krb1eidw	159	40	,	,	,
cord-345088-krb1eidw	159	41	and	and	CC
cord-345088-krb1eidw	159	42	were	be	VBD
cord-345088-krb1eidw	159	43	cultured	culture	VBN
cord-345088-krb1eidw	159	44	at	at	IN
cord-345088-krb1eidw	159	45	32	32	CD
cord-345088-krb1eidw	159	46	jC	jC	NNS
cord-345088-krb1eidw	160	1	(	(	-LRB-
cord-345088-krb1eidw	160	2	lanes	lane	NNS
cord-345088-krb1eidw	160	3	1	1	CD
cord-345088-krb1eidw	160	4	,	,	,
cord-345088-krb1eidw	160	5	3	3	CD
cord-345088-krb1eidw	160	6	,	,	,
cord-345088-krb1eidw	160	7	and	and	CC
cord-345088-krb1eidw	160	8	5	5	LS
cord-345088-krb1eidw	160	9	)	)	-RRB-
cord-345088-krb1eidw	160	10	or	or	CC
cord-345088-krb1eidw	160	11	40	40	CD
cord-345088-krb1eidw	160	12	jC	jC	NNP
cord-345088-krb1eidw	160	13	(	(	-LRB-
cord-345088-krb1eidw	160	14	lanes	lane	NNS
cord-345088-krb1eidw	160	15	2	2	CD
cord-345088-krb1eidw	160	16	,	,	,
cord-345088-krb1eidw	160	17	4	4	CD
cord-345088-krb1eidw	160	18	,	,	,
cord-345088-krb1eidw	160	19	and	and	CC
cord-345088-krb1eidw	160	20	6	6	CD
cord-345088-krb1eidw	160	21	)	)	-RRB-
cord-345088-krb1eidw	160	22	.	.	.
cord-345088-krb1eidw	161	1	The	the	DT
cord-345088-krb1eidw	161	2	expression	expression	NN
cord-345088-krb1eidw	161	3	of	of	IN
cord-345088-krb1eidw	161	4	the	the	DT
cord-345088-krb1eidw	161	5	S	S	NNP
cord-345088-krb1eidw	161	6	protein	protein	NN
cord-345088-krb1eidw	161	7	was	be	VBD
cord-345088-krb1eidw	161	8	examined	examine	VBN
cord-345088-krb1eidw	161	9	in	in	IN
cord-345088-krb1eidw	161	10	Western	western	JJ
cord-345088-krb1eidw	161	11	blot	blot	NN
cord-345088-krb1eidw	161	12	using	use	VBG
cord-345088-krb1eidw	161	13	anti	anti	JJ
cord-345088-krb1eidw	161	14	-	-	JJ
cord-345088-krb1eidw	161	15	IBV	IBV	NNP
cord-345088-krb1eidw	161	16	antibodies	antibody	NNS
cord-345088-krb1eidw	161	17	,	,	,
cord-345088-krb1eidw	161	18	and	and	CC
cord-345088-krb1eidw	161	19	h	h	NN
cord-345088-krb1eidw	161	20	-	-	HYPH
cord-345088-krb1eidw	161	21	tubulin	tubulin	NN
cord-345088-krb1eidw	161	22	was	be	VBD
cord-345088-krb1eidw	161	23	immunostained	immunostaine	VBN
cord-345088-krb1eidw	161	24	as	as	IN
cord-345088-krb1eidw	161	25	loading	loading	NN
cord-345088-krb1eidw	161	26	controls	control	NNS
cord-345088-krb1eidw	161	27	.	.	.
cord-345088-krb1eidw	162	1	(	(	-LRB-
cord-345088-krb1eidw	162	2	b	b	LS
cord-345088-krb1eidw	162	3	)	)	-RRB-
cord-345088-krb1eidw	162	4	The	the	DT
cord-345088-krb1eidw	162	5	membranefusion	membranefusion	NN
cord-345088-krb1eidw	162	6	activity	activity	NN
cord-345088-krb1eidw	162	7	of	of	IN
cord-345088-krb1eidw	162	8	the	the	DT
cord-345088-krb1eidw	162	9	S	S	NNP
cord-345088-krb1eidw	162	10	protein	protein	NN
cord-345088-krb1eidw	162	11	from	from	IN
cord-345088-krb1eidw	162	12	the	the	DT
cord-345088-krb1eidw	162	13	wt	wt	NN
cord-345088-krb1eidw	162	14	(	(	-LRB-
cord-345088-krb1eidw	162	15	panels	panel	NNS
cord-345088-krb1eidw	162	16	B	b	NN
cord-345088-krb1eidw	162	17	,	,	,
cord-345088-krb1eidw	162	18	E	E	NNP
cord-345088-krb1eidw	162	19	,	,	,
cord-345088-krb1eidw	162	20	and	and	CC
cord-345088-krb1eidw	162	21	H	H	NNP
cord-345088-krb1eidw	162	22	)	)	-RRB-
cord-345088-krb1eidw	162	23	and	and	CC
cord-345088-krb1eidw	162	24	ts	ts	NNP
cord-345088-krb1eidw	162	25	mutant	mutant	NN
cord-345088-krb1eidw	162	26	(	(	-LRB-
cord-345088-krb1eidw	162	27	panels	panel	NNS
cord-345088-krb1eidw	162	28	C	C	NNP
cord-345088-krb1eidw	162	29	,	,	,
cord-345088-krb1eidw	162	30	F	F	NNP
cord-345088-krb1eidw	162	31	,	,	,
cord-345088-krb1eidw	162	32	and	and	CC
cord-345088-krb1eidw	162	33	I	-PRON-	PRP
cord-345088-krb1eidw	162	34	)	)	-RRB-
cord-345088-krb1eidw	162	35	at	at	IN
cord-345088-krb1eidw	162	36	32	32	CD
cord-345088-krb1eidw	162	37	jC	jC	NNS
cord-345088-krb1eidw	163	1	(	(	-LRB-
cord-345088-krb1eidw	163	2	panels	panel	NNS
cord-345088-krb1eidw	163	3	B	B	NNP
cord-345088-krb1eidw	163	4	and	and	CC
cord-345088-krb1eidw	163	5	C	C	NNP
cord-345088-krb1eidw	163	6	)	)	-RRB-
cord-345088-krb1eidw	163	7	and	and	CC
cord-345088-krb1eidw	163	8	40	40	CD
cord-345088-krb1eidw	163	9	jC	jC	NNS
cord-345088-krb1eidw	163	10	(	(	-LRB-
cord-345088-krb1eidw	163	11	panels	panel	NNS
cord-345088-krb1eidw	163	12	C	c	NN
cord-345088-krb1eidw	163	13	and	and	CC
cord-345088-krb1eidw	163	14	F	F	NNP
cord-345088-krb1eidw	164	1	)	)	-RRB-
cord-345088-krb1eidw	164	2	2	2	CD
cord-345088-krb1eidw	164	3	days	day	NNS
cord-345088-krb1eidw	164	4	post	post	NN
cord-345088-krb1eidw	164	5	-	-	NN
cord-345088-krb1eidw	164	6	transfection	transfection	NN
cord-345088-krb1eidw	164	7	was	be	VBD
cord-345088-krb1eidw	164	8	compared	compare	VBN
cord-345088-krb1eidw	164	9	.	.	.
cord-345088-krb1eidw	165	1	Panels	panel	NNS
cord-345088-krb1eidw	165	2	H	H	NNP
cord-345088-krb1eidw	165	3	and	and	CC
cord-345088-krb1eidw	165	4	I	-PRON-	PRP
cord-345088-krb1eidw	165	5	show	show	VBP
cord-345088-krb1eidw	165	6	induction	induction	NN
cord-345088-krb1eidw	165	7	of	of	IN
cord-345088-krb1eidw	165	8	membrane	membrane	NN
cord-345088-krb1eidw	165	9	fusion	fusion	NN
cord-345088-krb1eidw	165	10	on	on	IN
cord-345088-krb1eidw	165	11	cells	cell	NNS
cord-345088-krb1eidw	165	12	transfected	transfecte	VBN
cord-345088-krb1eidw	165	13	with	with	IN
cord-345088-krb1eidw	165	14	the	the	DT
cord-345088-krb1eidw	165	15	wt	wt	NN
cord-345088-krb1eidw	165	16	and	and	CC
cord-345088-krb1eidw	165	17	ts291602	ts291602	NNP
cord-345088-krb1eidw	165	18	by	by	IN
cord-345088-krb1eidw	165	19	shifting	shift	VBG
cord-345088-krb1eidw	165	20	cells	cell	NNS
cord-345088-krb1eidw	165	21	shown	show	VBN
cord-345088-krb1eidw	165	22	in	in	IN
cord-345088-krb1eidw	165	23	panels	panel	NNS
cord-345088-krb1eidw	165	24	E	e	NN
cord-345088-krb1eidw	165	25	and	and	CC
cord-345088-krb1eidw	165	26	F	F	NNP
cord-345088-krb1eidw	165	27	to	to	IN
cord-345088-krb1eidw	165	28	32	32	CD
cord-345088-krb1eidw	165	29	jC	jC	NNP
cord-345088-krb1eidw	165	30	for	for	IN
cord-345088-krb1eidw	165	31	1	1	CD
cord-345088-krb1eidw	165	32	day	day	NN
cord-345088-krb1eidw	165	33	.	.	.
cord-345088-krb1eidw	166	1	Panels	panel	NNS
cord-345088-krb1eidw	166	2	A	A	NNP
cord-345088-krb1eidw	166	3	,	,	,
cord-345088-krb1eidw	166	4	D	D	NNP
cord-345088-krb1eidw	166	5	,	,	,
cord-345088-krb1eidw	166	6	and	and	CC
cord-345088-krb1eidw	166	7	G	g	NN
cord-345088-krb1eidw	166	8	show	show	NN
cord-345088-krb1eidw	166	9	cells	cell	NNS
cord-345088-krb1eidw	166	10	transfected	transfecte	VBN
cord-345088-krb1eidw	166	11	with	with	IN
cord-345088-krb1eidw	166	12	empty	empty	JJ
cord-345088-krb1eidw	166	13	plasmids	plasmid	NNS
cord-345088-krb1eidw	166	14	.	.	.
cord-345088-krb1eidw	167	1	reinforce	reinforce	VB
cord-345088-krb1eidw	167	2	the	the	DT
cord-345088-krb1eidw	167	3	conclusion	conclusion	NN
cord-345088-krb1eidw	167	4	that	that	IN
cord-345088-krb1eidw	167	5	the	the	DT
cord-345088-krb1eidw	167	6	majority	majority	NN
cord-345088-krb1eidw	167	7	of	of	IN
cord-345088-krb1eidw	167	8	the	the	DT
cord-345088-krb1eidw	167	9	S	S	NNP
cord-345088-krb1eidw	167	10	protein	protein	NN
cord-345088-krb1eidw	167	11	of	of	IN
cord-345088-krb1eidw	167	12	the	the	DT
cord-345088-krb1eidw	167	13	ts	ts	JJ
cord-345088-krb1eidw	167	14	mutants	mutant	NNS
cord-345088-krb1eidw	167	15	synthesized	synthesize	VBN
cord-345088-krb1eidw	167	16	at	at	IN
cord-345088-krb1eidw	167	17	the	the	DT
cord-345088-krb1eidw	167	18	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	167	19	temperature	temperature	NN
cord-345088-krb1eidw	167	20	may	may	MD
cord-345088-krb1eidw	167	21	represent	represent	VB
cord-345088-krb1eidw	167	22	a	a	DT
cord-345088-krb1eidw	167	23	pre	pre	JJ
cord-345088-krb1eidw	167	24	-	-	JJ
cord-345088-krb1eidw	167	25	mature	mature	JJ
cord-345088-krb1eidw	167	26	form	form	NN
cord-345088-krb1eidw	167	27	of	of	IN
cord-345088-krb1eidw	167	28	the	the	DT
cord-345088-krb1eidw	167	29	S	S	NNP
cord-345088-krb1eidw	167	30	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	167	31	.	.	.
cord-345088-krb1eidw	168	1	To	to	TO
cord-345088-krb1eidw	168	2	detect	detect	VB
cord-345088-krb1eidw	168	3	whether	whether	IN
cord-345088-krb1eidw	168	4	the	the	DT
cord-345088-krb1eidw	168	5	ts	ts	NNP
cord-345088-krb1eidw	168	6	mutant	mutant	NN
cord-345088-krb1eidw	168	7	S	S	NNP
cord-345088-krb1eidw	168	8	protein	protein	NN
cord-345088-krb1eidw	168	9	would	would	MD
cord-345088-krb1eidw	168	10	be	be	VB
cord-345088-krb1eidw	168	11	transported	transport	VBN
cord-345088-krb1eidw	168	12	to	to	IN
cord-345088-krb1eidw	168	13	the	the	DT
cord-345088-krb1eidw	168	14	plasma	plasma	NN
cord-345088-krb1eidw	168	15	membrane	membrane	NN
cord-345088-krb1eidw	168	16	at	at	IN
cord-345088-krb1eidw	168	17	the	the	DT
cord-345088-krb1eidw	168	18	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	168	19	temperature	temperature	NN
cord-345088-krb1eidw	168	20	,	,	,
cord-345088-krb1eidw	168	21	the	the	DT
cord-345088-krb1eidw	168	22	S	S	NNP
cord-345088-krb1eidw	168	23	protein	protein	NN
cord-345088-krb1eidw	168	24	was	be	VBD
cord-345088-krb1eidw	168	25	transiently	transiently	RB
cord-345088-krb1eidw	168	26	expressed	express	VBN
cord-345088-krb1eidw	168	27	in	in	IN
cord-345088-krb1eidw	168	28	Vero	Vero	NNP
cord-345088-krb1eidw	168	29	cells	cell	NNS
cord-345088-krb1eidw	168	30	using	use	VBG
cord-345088-krb1eidw	168	31	the	the	DT
cord-345088-krb1eidw	168	32	vaccinia	vaccinia	NN
cord-345088-krb1eidw	168	33	virus	virus	NN
cord-345088-krb1eidw	168	34	-	-	HYPH
cord-345088-krb1eidw	168	35	T7	t7	NN
cord-345088-krb1eidw	168	36	expression	expression	NN
cord-345088-krb1eidw	168	37	system	system	NN
cord-345088-krb1eidw	168	38	.	.	.
cord-345088-krb1eidw	169	1	The	the	DT
cord-345088-krb1eidw	169	2	transfected	transfecte	VBN
cord-345088-krb1eidw	169	3	cells	cell	NNS
cord-345088-krb1eidw	169	4	were	be	VBD
cord-345088-krb1eidw	169	5	treated	treat	VBN
cord-345088-krb1eidw	169	6	with	with	IN
cord-345088-krb1eidw	169	7	cycloheximide	cycloheximide	NN
cord-345088-krb1eidw	169	8	at	at	IN
cord-345088-krb1eidw	169	9	3.5	3.5	CD
cord-345088-krb1eidw	169	10	h	h	NN
cord-345088-krb1eidw	169	11	posttransfection	posttransfection	NN
cord-345088-krb1eidw	169	12	for	for	IN
cord-345088-krb1eidw	169	13	30	30	CD
cord-345088-krb1eidw	169	14	min	min	NN
cord-345088-krb1eidw	169	15	to	to	TO
cord-345088-krb1eidw	169	16	inhibit	inhibit	VB
cord-345088-krb1eidw	169	17	further	further	JJ
cord-345088-krb1eidw	169	18	protein	protein	NN
cord-345088-krb1eidw	169	19	synthesis	synthesis	NN
cord-345088-krb1eidw	169	20	.	.	.
cord-345088-krb1eidw	170	1	The	the	DT
cord-345088-krb1eidw	170	2	cells	cell	NNS
cord-345088-krb1eidw	170	3	were	be	VBD
cord-345088-krb1eidw	170	4	then	then	RB
cord-345088-krb1eidw	170	5	incubated	incubate	VBN
cord-345088-krb1eidw	170	6	at	at	IN
cord-345088-krb1eidw	170	7	32	32	CD
cord-345088-krb1eidw	170	8	and	and	CC
cord-345088-krb1eidw	170	9	40	40	CD
cord-345088-krb1eidw	170	10	jC	jC	NNS
cord-345088-krb1eidw	170	11	,	,	,
cord-345088-krb1eidw	170	12	respectively	respectively	RB
cord-345088-krb1eidw	170	13	.	.	.
cord-345088-krb1eidw	171	1	The	the	DT
cord-345088-krb1eidw	171	2	subcellular	subcellular	JJ
cord-345088-krb1eidw	171	3	localization	localization	NN
cord-345088-krb1eidw	171	4	of	of	IN
cord-345088-krb1eidw	171	5	the	the	DT
cord-345088-krb1eidw	171	6	S	S	NNP
cord-345088-krb1eidw	171	7	protein	protein	NN
cord-345088-krb1eidw	171	8	was	be	VBD
cord-345088-krb1eidw	171	9	analyzed	analyze	VBN
cord-345088-krb1eidw	171	10	by	by	IN
cord-345088-krb1eidw	171	11	indirect	indirect	JJ
cord-345088-krb1eidw	171	12	immunofluorescent	immunofluorescent	NN
cord-345088-krb1eidw	171	13	staining	stain	VBG
cord-345088-krb1eidw	171	14	at	at	IN
cord-345088-krb1eidw	171	15	4	4	CD
cord-345088-krb1eidw	171	16	,	,	,
cord-345088-krb1eidw	171	17	8	8	CD
cord-345088-krb1eidw	171	18	,	,	,
cord-345088-krb1eidw	171	19	16	16	CD
cord-345088-krb1eidw	171	20	,	,	,
cord-345088-krb1eidw	171	21	and	and	CC
cord-345088-krb1eidw	171	22	24	24	CD
cord-345088-krb1eidw	171	23	h	h	NN
cord-345088-krb1eidw	171	24	postinfection	postinfection	NN
cord-345088-krb1eidw	171	25	,	,	,
cord-345088-krb1eidw	171	26	respectively	respectively	RB
cord-345088-krb1eidw	171	27	.	.	.
cord-345088-krb1eidw	172	1	The	the	DT
cord-345088-krb1eidw	172	2	confocal	confocal	JJ
cord-345088-krb1eidw	172	3	microscopy	microscopy	NN
cord-345088-krb1eidw	172	4	images	image	NNS
cord-345088-krb1eidw	172	5	of	of	IN
cord-345088-krb1eidw	172	6	transfected	transfecte	VBN
cord-345088-krb1eidw	172	7	cells	cell	NNS
cord-345088-krb1eidw	172	8	are	be	VBP
cord-345088-krb1eidw	172	9	shown	show	VBN
cord-345088-krb1eidw	172	10	in	in	IN
cord-345088-krb1eidw	172	11	Fig	Fig	NNP
cord-345088-krb1eidw	172	12	.	.	.
cord-345088-krb1eidw	173	1	4	4	LS
cord-345088-krb1eidw	173	2	.	.	.
cord-345088-krb1eidw	174	1	Plasma	plasma	NN
cord-345088-krb1eidw	174	2	membrane	membrane	NN
cord-345088-krb1eidw	174	3	staining	staining	NN
cord-345088-krb1eidw	174	4	of	of	IN
cord-345088-krb1eidw	174	5	the	the	DT
cord-345088-krb1eidw	174	6	ts	ts	JJ
cord-345088-krb1eidw	174	7	mutant	mutant	NN
cord-345088-krb1eidw	174	8	S	S	NNP
cord-345088-krb1eidw	174	9	protein	protein	NN
cord-345088-krb1eidw	174	10	was	be	VBD
cord-345088-krb1eidw	174	11	not	not	RB
cord-345088-krb1eidw	174	12	observed	observe	VBN
cord-345088-krb1eidw	174	13	at	at	IN
cord-345088-krb1eidw	174	14	any	any	DT
cord-345088-krb1eidw	174	15	time	time	NN
cord-345088-krb1eidw	174	16	points	point	VBZ
cord-345088-krb1eidw	174	17	posttransfection	posttransfection	NN
cord-345088-krb1eidw	174	18	at	at	IN
cord-345088-krb1eidw	174	19	40	40	CD
cord-345088-krb1eidw	174	20	jC	jC	NNS
cord-345088-krb1eidw	174	21	,	,	,
cord-345088-krb1eidw	174	22	but	but	CC
cord-345088-krb1eidw	174	23	was	be	VBD
cord-345088-krb1eidw	174	24	clearly	clearly	RB
cord-345088-krb1eidw	174	25	seen	see	VBN
cord-345088-krb1eidw	174	26	at	at	IN
cord-345088-krb1eidw	174	27	32	32	CD
cord-345088-krb1eidw	174	28	jC	jC	NNS
cord-345088-krb1eidw	174	29	at	at	IN
cord-345088-krb1eidw	174	30	16	16	CD
cord-345088-krb1eidw	174	31	and	and	CC
cord-345088-krb1eidw	174	32	24	24	CD
cord-345088-krb1eidw	174	33	h	h	NN
cord-345088-krb1eidw	174	34	postinfection	postinfection	NN
cord-345088-krb1eidw	174	35	(	(	-LRB-
cord-345088-krb1eidw	174	36	Fig	Fig	NNP
cord-345088-krb1eidw	174	37	.	.	NNP
cord-345088-krb1eidw	174	38	4	4	CD
cord-345088-krb1eidw	174	39	)	)	-RRB-
cord-345088-krb1eidw	174	40	.	.	.
cord-345088-krb1eidw	175	1	The	the	DT
cord-345088-krb1eidw	175	2	revertant	revertant	NN
cord-345088-krb1eidw	175	3	S	s	NN
cord-345088-krb1eidw	175	4	protein	protein	NN
cord-345088-krb1eidw	175	5	was	be	VBD
cord-345088-krb1eidw	175	6	expressed	express	VBN
cord-345088-krb1eidw	175	7	on	on	IN
cord-345088-krb1eidw	175	8	the	the	DT
cord-345088-krb1eidw	175	9	cell	cell	NN
cord-345088-krb1eidw	175	10	surface	surface	NN
cord-345088-krb1eidw	175	11	at	at	IN
cord-345088-krb1eidw	175	12	both	both	DT
cord-345088-krb1eidw	175	13	temperatures	temperature	NNS
cord-345088-krb1eidw	175	14	at	at	IN
cord-345088-krb1eidw	175	15	16	16	CD
cord-345088-krb1eidw	175	16	and	and	CC
cord-345088-krb1eidw	175	17	24	24	CD
cord-345088-krb1eidw	175	18	h	h	NN
cord-345088-krb1eidw	175	19	posttransfection	posttransfection	NN
cord-345088-krb1eidw	175	20	.	.	.
cord-345088-krb1eidw	176	1	These	these	DT
cord-345088-krb1eidw	176	2	results	result	NNS
cord-345088-krb1eidw	176	3	may	may	MD
cord-345088-krb1eidw	176	4	explain	explain	VB
cord-345088-krb1eidw	176	5	the	the	DT
cord-345088-krb1eidw	176	6	lack	lack	NN
cord-345088-krb1eidw	176	7	of	of	IN
cord-345088-krb1eidw	176	8	membrane	membrane	NN
cord-345088-krb1eidw	176	9	fusion	fusion	NN
cord-345088-krb1eidw	176	10	activity	activity	NN
cord-345088-krb1eidw	176	11	of	of	IN
cord-345088-krb1eidw	176	12	the	the	DT
cord-345088-krb1eidw	176	13	ts	ts	NNP
cord-345088-krb1eidw	176	14	mutant	mutant	NN
cord-345088-krb1eidw	176	15	S	S	NNP
cord-345088-krb1eidw	176	16	protein	protein	NN
cord-345088-krb1eidw	176	17	in	in	IN
cord-345088-krb1eidw	176	18	both	both	CC
cord-345088-krb1eidw	176	19	infected	infect	VBN
cord-345088-krb1eidw	176	20	and	and	CC
cord-345088-krb1eidw	176	21	transfected	transfecte	VBN
cord-345088-krb1eidw	176	22	cells	cell	NNS
cord-345088-krb1eidw	176	23	.	.	.
cord-345088-krb1eidw	177	1	Endo	endo	NN
cord-345088-krb1eidw	177	2	-	-	HYPH
cord-345088-krb1eidw	177	3	H	H	NNP
cord-345088-krb1eidw	177	4	treatment	treatment	NN
cord-345088-krb1eidw	177	5	of	of	IN
cord-345088-krb1eidw	177	6	the	the	DT
cord-345088-krb1eidw	177	7	S	S	NNP
cord-345088-krb1eidw	177	8	protein	protein	NN
cord-345088-krb1eidw	177	9	from	from	IN
cord-345088-krb1eidw	177	10	rev-1	rev-1	NNP
cord-345088-krb1eidw	177	11	and	and	CC
cord-345088-krb1eidw	177	12	ts291602	ts291602	NNP
cord-345088-krb1eidw	177	13	.	.	.
cord-345088-krb1eidw	178	1	The	the	DT
cord-345088-krb1eidw	178	2	S	S	NNP
cord-345088-krb1eidw	178	3	protein	protein	NN
cord-345088-krb1eidw	178	4	derived	derive	VBN
cord-345088-krb1eidw	178	5	from	from	IN
cord-345088-krb1eidw	178	6	rev-1	rev-1	NNP
cord-345088-krb1eidw	178	7	(	(	-LRB-
cord-345088-krb1eidw	178	8	lanes	lane	NNS
cord-345088-krb1eidw	178	9	5	5	CD
cord-345088-krb1eidw	178	10	,	,	,
cord-345088-krb1eidw	178	11	6	6	CD
cord-345088-krb1eidw	178	12	,	,	,
cord-345088-krb1eidw	178	13	7	7	CD
cord-345088-krb1eidw	178	14	,	,	,
cord-345088-krb1eidw	178	15	and	and	CC
cord-345088-krb1eidw	178	16	8)	8)	NN
cord-345088-krb1eidw	178	17	and	and	CC
cord-345088-krb1eidw	178	18	ts291602	ts291602	NNP
cord-345088-krb1eidw	178	19	(	(	-LRB-
cord-345088-krb1eidw	178	20	lanes	lane	NNS
cord-345088-krb1eidw	178	21	9	9	CD
cord-345088-krb1eidw	178	22	,	,	,
cord-345088-krb1eidw	178	23	10	10	CD
cord-345088-krb1eidw	178	24	,	,	,
cord-345088-krb1eidw	178	25	11	11	CD
cord-345088-krb1eidw	178	26	,	,	,
cord-345088-krb1eidw	178	27	and	and	CC
cord-345088-krb1eidw	178	28	12	12	CD
cord-345088-krb1eidw	178	29	)	)	-RRB-
cord-345088-krb1eidw	178	30	were	be	VBD
cord-345088-krb1eidw	178	31	expressed	express	VBN
cord-345088-krb1eidw	178	32	in	in	IN
cord-345088-krb1eidw	178	33	Vero	Vero	NNP
cord-345088-krb1eidw	178	34	cells	cell	NNS
cord-345088-krb1eidw	178	35	at	at	IN
cord-345088-krb1eidw	178	36	32	32	CD
cord-345088-krb1eidw	178	37	jC	jC	NNP
cord-345088-krb1eidw	178	38	(	(	-LRB-
cord-345088-krb1eidw	178	39	lanes	lane	NNS
cord-345088-krb1eidw	178	40	5	5	CD
cord-345088-krb1eidw	178	41	,	,	,
cord-345088-krb1eidw	178	42	6	6	CD
cord-345088-krb1eidw	178	43	,	,	,
cord-345088-krb1eidw	178	44	9	9	CD
cord-345088-krb1eidw	178	45	,	,	,
cord-345088-krb1eidw	178	46	and	and	CC
cord-345088-krb1eidw	178	47	10	10	CD
cord-345088-krb1eidw	178	48	)	)	-RRB-
cord-345088-krb1eidw	178	49	and	and	CC
cord-345088-krb1eidw	178	50	40	40	CD
cord-345088-krb1eidw	178	51	jC	jC	NNP
cord-345088-krb1eidw	178	52	(	(	-LRB-
cord-345088-krb1eidw	178	53	lanes	lane	NNS
cord-345088-krb1eidw	178	54	7	7	CD
cord-345088-krb1eidw	178	55	,	,	,
cord-345088-krb1eidw	178	56	8	8	CD
cord-345088-krb1eidw	178	57	,	,	,
cord-345088-krb1eidw	178	58	11	11	CD
cord-345088-krb1eidw	178	59	,	,	,
cord-345088-krb1eidw	178	60	and	and	CC
cord-345088-krb1eidw	178	61	12	12	CD
cord-345088-krb1eidw	178	62	)	)	-RRB-
cord-345088-krb1eidw	178	63	,	,	,
cord-345088-krb1eidw	178	64	using	use	VBG
cord-345088-krb1eidw	178	65	a	a	DT
cord-345088-krb1eidw	178	66	T7-vaccinia	t7-vaccinia	FW
cord-345088-krb1eidw	178	67	expression	expression	NN
cord-345088-krb1eidw	178	68	system	system	NN
cord-345088-krb1eidw	178	69	.	.	.
cord-345088-krb1eidw	179	1	The	the	DT
cord-345088-krb1eidw	179	2	radiolabeled	radiolabeled	JJ
cord-345088-krb1eidw	179	3	proteins	protein	NNS
cord-345088-krb1eidw	179	4	were	be	VBD
cord-345088-krb1eidw	179	5	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	179	6	with	with	IN
cord-345088-krb1eidw	179	7	anti	anti	JJ
cord-345088-krb1eidw	179	8	-	-	JJ
cord-345088-krb1eidw	179	9	IBV	IBV	NNP
cord-345088-krb1eidw	179	10	antibodies	antibody	NNS
cord-345088-krb1eidw	179	11	,	,	,
cord-345088-krb1eidw	179	12	the	the	DT
cord-345088-krb1eidw	179	13	eluted	elute	VBN
cord-345088-krb1eidw	179	14	proteins	protein	NNS
cord-345088-krb1eidw	179	15	were	be	VBD
cord-345088-krb1eidw	179	16	Endo	Endo	NNP
cord-345088-krb1eidw	179	17	-	-	HYPH
cord-345088-krb1eidw	179	18	H-(lanes	H-(lanes	NNP
cord-345088-krb1eidw	179	19	6	6	CD
cord-345088-krb1eidw	179	20	,	,	,
cord-345088-krb1eidw	179	21	8	8	CD
cord-345088-krb1eidw	179	22	,	,	,
cord-345088-krb1eidw	179	23	10	10	CD
cord-345088-krb1eidw	179	24	,	,	,
cord-345088-krb1eidw	179	25	and	and	CC
cord-345088-krb1eidw	179	26	12	12	CD
cord-345088-krb1eidw	179	27	)	)	-RRB-
cord-345088-krb1eidw	179	28	or	or	CC
cord-345088-krb1eidw	179	29	mock	mock	RB
cord-345088-krb1eidw	179	30	-	-	HYPH
cord-345088-krb1eidw	179	31	treated	treated	JJ
cord-345088-krb1eidw	179	32	(	(	-LRB-
cord-345088-krb1eidw	179	33	lanes	lane	NNS
cord-345088-krb1eidw	179	34	5	5	CD
cord-345088-krb1eidw	179	35	,	,	,
cord-345088-krb1eidw	179	36	7	7	CD
cord-345088-krb1eidw	179	37	,	,	,
cord-345088-krb1eidw	179	38	9	9	CD
cord-345088-krb1eidw	179	39	,	,	,
cord-345088-krb1eidw	179	40	and	and	CC
cord-345088-krb1eidw	179	41	11	11	CD
cord-345088-krb1eidw	179	42	)	)	-RRB-
cord-345088-krb1eidw	179	43	and	and	CC
cord-345088-krb1eidw	179	44	analyzed	analyze	VBN
cord-345088-krb1eidw	179	45	by	by	IN
cord-345088-krb1eidw	179	46	SDS	SDS	NNP
cord-345088-krb1eidw	179	47	-	-	HYPH
cord-345088-krb1eidw	179	48	PAGE	PAGE	NNP
cord-345088-krb1eidw	179	49	.	.	.
cord-345088-krb1eidw	180	1	(	(	-LRB-
cord-345088-krb1eidw	180	2	b	b	LS
cord-345088-krb1eidw	180	3	)	)	-RRB-
cord-345088-krb1eidw	180	4	Detection	detection	NN
cord-345088-krb1eidw	180	5	of	of	IN
cord-345088-krb1eidw	180	6	the	the	DT
cord-345088-krb1eidw	180	7	less	less	RBR
cord-345088-krb1eidw	180	8	matured	matured	JJ
cord-345088-krb1eidw	180	9	S	S	NNP
cord-345088-krb1eidw	180	10	protein	protein	NN
cord-345088-krb1eidw	180	11	from	from	IN
cord-345088-krb1eidw	180	12	ts291602	ts291602	NNP
cord-345088-krb1eidw	180	13	at	at	IN
cord-345088-krb1eidw	180	14	40	40	CD
cord-345088-krb1eidw	180	15	jC	jC	NNS
cord-345088-krb1eidw	180	16	and	and	CC
cord-345088-krb1eidw	180	17	the	the	DT
cord-345088-krb1eidw	180	18	defect	defect	NN
cord-345088-krb1eidw	180	19	in	in	IN
cord-345088-krb1eidw	180	20	cleavage	cleavage	NN
cord-345088-krb1eidw	180	21	of	of	IN
cord-345088-krb1eidw	180	22	the	the	DT
cord-345088-krb1eidw	180	23	mutant	mutant	JJ
cord-345088-krb1eidw	180	24	S	S	NNP
cord-345088-krb1eidw	180	25	protein	protein	NN
cord-345088-krb1eidw	180	26	.	.	.
cord-345088-krb1eidw	181	1	Cells	cell	NNS
cord-345088-krb1eidw	181	2	were	be	VBD
cord-345088-krb1eidw	181	3	infected	infect	VBN
cord-345088-krb1eidw	181	4	with	with	IN
cord-345088-krb1eidw	181	5	ts291602	ts291602	NNP
cord-345088-krb1eidw	181	6	and	and	CC
cord-345088-krb1eidw	181	7	rev-1	rev-1	NNP
cord-345088-krb1eidw	181	8	for	for	IN
cord-345088-krb1eidw	181	9	5	5	CD
cord-345088-krb1eidw	181	10	h	h	NN
cord-345088-krb1eidw	181	11	at	at	IN
cord-345088-krb1eidw	181	12	32	32	CD
cord-345088-krb1eidw	181	13	jC	jC	NNP
cord-345088-krb1eidw	181	14	,	,	,
cord-345088-krb1eidw	181	15	one	one	CD
cord-345088-krb1eidw	181	16	of	of	IN
cord-345088-krb1eidw	181	17	the	the	DT
cord-345088-krb1eidw	181	18	duplicates	duplicate	NNS
cord-345088-krb1eidw	181	19	was	be	VBD
cord-345088-krb1eidw	181	20	maintained	maintain	VBN
cord-345088-krb1eidw	181	21	at	at	IN
cord-345088-krb1eidw	181	22	32	32	CD
cord-345088-krb1eidw	181	23	jC	jC	NNP
cord-345088-krb1eidw	181	24	(	(	-LRB-
cord-345088-krb1eidw	181	25	lanes	lane	NNS
cord-345088-krb1eidw	181	26	1	1	CD
cord-345088-krb1eidw	181	27	,	,	,
cord-345088-krb1eidw	181	28	3	3	CD
cord-345088-krb1eidw	181	29	,	,	,
cord-345088-krb1eidw	181	30	5	5	CD
cord-345088-krb1eidw	181	31	,	,	,
cord-345088-krb1eidw	181	32	and	and	CC
cord-345088-krb1eidw	181	33	7	7	CD
cord-345088-krb1eidw	181	34	)	)	-RRB-
cord-345088-krb1eidw	181	35	,	,	,
cord-345088-krb1eidw	181	36	and	and	CC
cord-345088-krb1eidw	181	37	the	the	DT
cord-345088-krb1eidw	181	38	other	other	JJ
cord-345088-krb1eidw	181	39	one	one	NN
cord-345088-krb1eidw	181	40	was	be	VBD
cord-345088-krb1eidw	181	41	shifted	shift	VBN
cord-345088-krb1eidw	181	42	to	to	IN
cord-345088-krb1eidw	181	43	40	40	CD
cord-345088-krb1eidw	181	44	jC	jC	NNP
cord-345088-krb1eidw	181	45	(	(	-LRB-
cord-345088-krb1eidw	181	46	lanes	lane	NNS
cord-345088-krb1eidw	181	47	2	2	CD
cord-345088-krb1eidw	181	48	,	,	,
cord-345088-krb1eidw	181	49	4	4	CD
cord-345088-krb1eidw	181	50	,	,	,
cord-345088-krb1eidw	181	51	6	6	CD
cord-345088-krb1eidw	181	52	,	,	,
cord-345088-krb1eidw	181	53	and	and	CC
cord-345088-krb1eidw	181	54	8)	8)	NN
cord-345088-krb1eidw	181	55	.	.	.
cord-345088-krb1eidw	182	1	Cells	cell	NNS
cord-345088-krb1eidw	182	2	were	be	VBD
cord-345088-krb1eidw	182	3	radiolabeled	radiolabele	VBN
cord-345088-krb1eidw	182	4	and	and	CC
cord-345088-krb1eidw	182	5	viral	viral	JJ
cord-345088-krb1eidw	182	6	proteins	protein	NNS
cord-345088-krb1eidw	182	7	were	be	VBD
cord-345088-krb1eidw	182	8	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	182	9	with	with	IN
cord-345088-krb1eidw	182	10	anti	anti	JJ
cord-345088-krb1eidw	182	11	-	-	JJ
cord-345088-krb1eidw	182	12	IBV	IBV	NNP
cord-345088-krb1eidw	182	13	antibodies	antibody	NNS
cord-345088-krb1eidw	182	14	and	and	CC
cord-345088-krb1eidw	182	15	analyzed	analyze	VBN
cord-345088-krb1eidw	182	16	by	by	IN
cord-345088-krb1eidw	182	17	SDS	SDS	NNP
cord-345088-krb1eidw	182	18	-	-	HYPH
cord-345088-krb1eidw	182	19	PAGE	PAGE	NNP
cord-345088-krb1eidw	182	20	.	.	.
cord-345088-krb1eidw	183	1	Lanes	lane	NNS
cord-345088-krb1eidw	183	2	1	1	CD
cord-345088-krb1eidw	183	3	,	,	,
cord-345088-krb1eidw	183	4	2	2	CD
cord-345088-krb1eidw	183	5	,	,	,
cord-345088-krb1eidw	183	6	3	3	CD
cord-345088-krb1eidw	183	7	,	,	,
cord-345088-krb1eidw	183	8	and	and	CC
cord-345088-krb1eidw	183	9	4	4	CD
cord-345088-krb1eidw	183	10	refer	refer	VBP
cord-345088-krb1eidw	183	11	to	to	IN
cord-345088-krb1eidw	183	12	viral	viral	JJ
cord-345088-krb1eidw	183	13	products	product	NNS
cord-345088-krb1eidw	183	14	detected	detect	VBN
cord-345088-krb1eidw	183	15	from	from	IN
cord-345088-krb1eidw	183	16	cell	cell	NN
cord-345088-krb1eidw	183	17	lysates	lysate	NNS
cord-345088-krb1eidw	183	18	and	and	CC
cord-345088-krb1eidw	183	19	lanes	lane	NNS
cord-345088-krb1eidw	183	20	5	5	CD
cord-345088-krb1eidw	183	21	,	,	,
cord-345088-krb1eidw	183	22	6	6	CD
cord-345088-krb1eidw	183	23	,	,	,
cord-345088-krb1eidw	183	24	7	7	CD
cord-345088-krb1eidw	183	25	,	,	,
cord-345088-krb1eidw	183	26	and	and	CC
cord-345088-krb1eidw	183	27	8	8	CD
cord-345088-krb1eidw	183	28	refer	refer	NN
cord-345088-krb1eidw	183	29	to	to	IN
cord-345088-krb1eidw	183	30	viral	viral	JJ
cord-345088-krb1eidw	183	31	proteins	protein	NNS
cord-345088-krb1eidw	183	32	detected	detect	VBN
cord-345088-krb1eidw	183	33	from	from	IN
cord-345088-krb1eidw	183	34	virus	virus	NN
cord-345088-krb1eidw	183	35	particles	particle	NNS
cord-345088-krb1eidw	183	36	released	release	VBN
cord-345088-krb1eidw	183	37	to	to	IN
cord-345088-krb1eidw	183	38	the	the	DT
cord-345088-krb1eidw	183	39	cultured	cultured	JJ
cord-345088-krb1eidw	183	40	media	medium	NNS
cord-345088-krb1eidw	183	41	.	.	.
cord-345088-krb1eidw	184	1	Quantitative	quantitative	JJ
cord-345088-krb1eidw	184	2	analysis	analysis	NN
cord-345088-krb1eidw	184	3	of	of	IN
cord-345088-krb1eidw	184	4	surface	surface	NN
cord-345088-krb1eidw	184	5	expression	expression	NN
cord-345088-krb1eidw	184	6	of	of	IN
cord-345088-krb1eidw	184	7	the	the	DT
cord-345088-krb1eidw	184	8	wild	wild	JJ
cord-345088-krb1eidw	184	9	type	type	NN
cord-345088-krb1eidw	184	10	and	and	CC
cord-345088-krb1eidw	184	11	ts	ts	NN
cord-345088-krb1eidw	184	12	mutant	mutant	NNP
cord-345088-krb1eidw	184	13	S	S	NNP
cord-345088-krb1eidw	184	14	protein	protein	NN
cord-345088-krb1eidw	184	15	was	be	VBD
cord-345088-krb1eidw	184	16	carried	carry	VBN
cord-345088-krb1eidw	184	17	out	out	RP
cord-345088-krb1eidw	184	18	by	by	IN
cord-345088-krb1eidw	184	19	immunofluorescent	immunofluorescent	NN
cord-345088-krb1eidw	184	20	staining	stain	VBG
cord-345088-krb1eidw	184	21	with	with	IN
cord-345088-krb1eidw	184	22	anti	anti	JJ
cord-345088-krb1eidw	184	23	-	-	JJ
cord-345088-krb1eidw	184	24	S	s	JJ
cord-345088-krb1eidw	184	25	protein	protein	NN
cord-345088-krb1eidw	184	26	antiserum	antiserum	NN
cord-345088-krb1eidw	184	27	and	and	CC
cord-345088-krb1eidw	184	28	the	the	DT
cord-345088-krb1eidw	184	29	positive	positive	JJ
cord-345088-krb1eidw	184	30	staining	staining	NN
cord-345088-krb1eidw	184	31	cells	cell	NNS
cord-345088-krb1eidw	184	32	were	be	VBD
cord-345088-krb1eidw	184	33	sorted	sort	VBN
cord-345088-krb1eidw	184	34	by	by	IN
cord-345088-krb1eidw	184	35	flow	flow	NN
cord-345088-krb1eidw	184	36	cytometry	cytometry	NN
cord-345088-krb1eidw	184	37	.	.	.
cord-345088-krb1eidw	185	1	As	as	IN
cord-345088-krb1eidw	185	2	shown	show	VBN
cord-345088-krb1eidw	185	3	in	in	IN
cord-345088-krb1eidw	185	4	Fig	Fig	NNP
cord-345088-krb1eidw	185	5	.	.	.
cord-345088-krb1eidw	186	1	5	5	CD
cord-345088-krb1eidw	186	2	,	,	,
cord-345088-krb1eidw	186	3	5.5	5.5	CD
cord-345088-krb1eidw	186	4	%	%	NN
cord-345088-krb1eidw	186	5	of	of	IN
cord-345088-krb1eidw	186	6	nonpermeabilizing	nonpermeabilize	VBG
cord-345088-krb1eidw	186	7	(	(	-LRB-
cord-345088-krb1eidw	186	8	panel	panel	NN
cord-345088-krb1eidw	186	9	A	A	NNP
cord-345088-krb1eidw	186	10	)	)	-RRB-
cord-345088-krb1eidw	186	11	and	and	CC
cord-345088-krb1eidw	186	12	0.7	0.7	CD
cord-345088-krb1eidw	186	13	%	%	NN
cord-345088-krb1eidw	186	14	of	of	IN
cord-345088-krb1eidw	186	15	permeabilizing	permeabilizing	NN
cord-345088-krb1eidw	186	16	(	(	-LRB-
cord-345088-krb1eidw	186	17	panel	panel	NN
cord-345088-krb1eidw	186	18	F	F	NNP
cord-345088-krb1eidw	186	19	)	)	-RRB-
cord-345088-krb1eidw	186	20	cells	cell	NNS
cord-345088-krb1eidw	186	21	expressing	express	VBG
cord-345088-krb1eidw	186	22	the	the	DT
cord-345088-krb1eidw	186	23	empty	empty	JJ
cord-345088-krb1eidw	186	24	plasmid	plasmid	NN
cord-345088-krb1eidw	186	25	showed	show	VBD
cord-345088-krb1eidw	186	26	background	background	NN
cord-345088-krb1eidw	186	27	staining	staining	NN
cord-345088-krb1eidw	186	28	.	.	.
cord-345088-krb1eidw	187	1	When	when	WRB
cord-345088-krb1eidw	187	2	the	the	DT
cord-345088-krb1eidw	187	3	cells	cell	NNS
cord-345088-krb1eidw	187	4	were	be	VBD
cord-345088-krb1eidw	187	5	incubated	incubate	VBN
cord-345088-krb1eidw	187	6	at	at	IN
cord-345088-krb1eidw	187	7	the	the	DT
cord-345088-krb1eidw	187	8	permissive	permissive	JJ
cord-345088-krb1eidw	187	9	temperature	temperature	NN
cord-345088-krb1eidw	187	10	,	,	,
cord-345088-krb1eidw	187	11	3.8	3.8	CD
cord-345088-krb1eidw	187	12	%	%	NN
cord-345088-krb1eidw	187	13	(	(	-LRB-
cord-345088-krb1eidw	187	14	9.2	9.2	CD
cord-345088-krb1eidw	187	15	-5.5	-5.5	NFP
cord-345088-krb1eidw	187	16	)	)	-RRB-
cord-345088-krb1eidw	187	17	of	of	IN
cord-345088-krb1eidw	187	18	HeLa	HeLa	NNP
cord-345088-krb1eidw	187	19	cells	cell	NNS
cord-345088-krb1eidw	187	20	expressing	express	VBG
cord-345088-krb1eidw	187	21	the	the	DT
cord-345088-krb1eidw	187	22	wt	wt	NN
cord-345088-krb1eidw	187	23	S	S	NNP
cord-345088-krb1eidw	187	24	protein	protein	NN
cord-345088-krb1eidw	187	25	(	(	-LRB-
cord-345088-krb1eidw	187	26	panel	panel	NN
cord-345088-krb1eidw	187	27	B	B	NNP
cord-345088-krb1eidw	187	28	)	)	-RRB-
cord-345088-krb1eidw	187	29	and	and	CC
cord-345088-krb1eidw	187	30	4.1	4.1	CD
cord-345088-krb1eidw	187	31	%	%	NN
cord-345088-krb1eidw	187	32	(	(	-LRB-
cord-345088-krb1eidw	187	33	9.6	9.6	CD
cord-345088-krb1eidw	187	34	-5.5	-5.5	ADD
cord-345088-krb1eidw	187	35	)	)	-RRB-
cord-345088-krb1eidw	187	36	of	of	IN
cord-345088-krb1eidw	187	37	cells	cell	NNS
cord-345088-krb1eidw	187	38	expressing	express	VBG
cord-345088-krb1eidw	187	39	the	the	DT
cord-345088-krb1eidw	187	40	ts	ts	NNP
cord-345088-krb1eidw	187	41	mutant	mutant	NN
cord-345088-krb1eidw	187	42	S	S	NNP
cord-345088-krb1eidw	187	43	protein	protein	NN
cord-345088-krb1eidw	187	44	(	(	-LRB-
cord-345088-krb1eidw	187	45	panel	panel	NN
cord-345088-krb1eidw	187	46	C	C	NNP
cord-345088-krb1eidw	187	47	)	)	-RRB-
cord-345088-krb1eidw	187	48	displayed	display	VBD
cord-345088-krb1eidw	187	49	surface	surface	NN
cord-345088-krb1eidw	187	50	staining	staining	NN
cord-345088-krb1eidw	187	51	.	.	.
cord-345088-krb1eidw	188	1	After	after	IN
cord-345088-krb1eidw	188	2	permeabilizing	permeabilize	VBG
cord-345088-krb1eidw	188	3	with	with	IN
cord-345088-krb1eidw	188	4	0.1	0.1	CD
cord-345088-krb1eidw	188	5	%	%	NN
cord-345088-krb1eidw	188	6	saponin	saponin	NN
cord-345088-krb1eidw	188	7	,	,	,
cord-345088-krb1eidw	188	8	11.8	11.8	CD
cord-345088-krb1eidw	188	9	%	%	NN
cord-345088-krb1eidw	188	10	(	(	-LRB-
cord-345088-krb1eidw	188	11	12.5	12.5	CD
cord-345088-krb1eidw	188	12	-0.7	-0.7	JJ
cord-345088-krb1eidw	188	13	)	)	-RRB-
cord-345088-krb1eidw	188	14	of	of	IN
cord-345088-krb1eidw	188	15	cells	cell	NNS
cord-345088-krb1eidw	188	16	expressing	express	VBG
cord-345088-krb1eidw	188	17	the	the	DT
cord-345088-krb1eidw	188	18	wt	wt	NN
cord-345088-krb1eidw	188	19	S	S	NNP
cord-345088-krb1eidw	188	20	protein	protein	NN
cord-345088-krb1eidw	188	21	(	(	-LRB-
cord-345088-krb1eidw	188	22	panel	panel	NN
cord-345088-krb1eidw	188	23	G	G	NNP
cord-345088-krb1eidw	188	24	)	)	-RRB-
cord-345088-krb1eidw	188	25	and	and	CC
cord-345088-krb1eidw	188	26	10.9	10.9	CD
cord-345088-krb1eidw	188	27	%	%	NN
cord-345088-krb1eidw	188	28	(	(	-LRB-
cord-345088-krb1eidw	188	29	11.6	11.6	CD
cord-345088-krb1eidw	188	30	-	-	SYM
cord-345088-krb1eidw	188	31	0.7	0.7	CD
cord-345088-krb1eidw	188	32	)	)	-RRB-
cord-345088-krb1eidw	188	33	of	of	IN
cord-345088-krb1eidw	188	34	cells	cell	NNS
cord-345088-krb1eidw	188	35	expressing	express	VBG
cord-345088-krb1eidw	188	36	the	the	DT
cord-345088-krb1eidw	188	37	ts	ts	NNP
cord-345088-krb1eidw	188	38	mutant	mutant	NN
cord-345088-krb1eidw	188	39	S	S	NNP
cord-345088-krb1eidw	188	40	protein	protein	NN
cord-345088-krb1eidw	188	41	(	(	-LRB-
cord-345088-krb1eidw	188	42	panel	panel	NN
cord-345088-krb1eidw	188	43	H	H	NNP
cord-345088-krb1eidw	188	44	)	)	-RRB-
cord-345088-krb1eidw	188	45	showed	show	VBD
cord-345088-krb1eidw	188	46	positive	positive	JJ
cord-345088-krb1eidw	188	47	staining	staining	NN
cord-345088-krb1eidw	188	48	.	.	.
cord-345088-krb1eidw	189	1	When	when	WRB
cord-345088-krb1eidw	189	2	the	the	DT
cord-345088-krb1eidw	189	3	cells	cell	NNS
cord-345088-krb1eidw	189	4	were	be	VBD
cord-345088-krb1eidw	189	5	incubated	incubate	VBN
cord-345088-krb1eidw	189	6	at	at	IN
cord-345088-krb1eidw	189	7	the	the	DT
cord-345088-krb1eidw	189	8	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	189	9	temperature	temperature	NN
cord-345088-krb1eidw	189	10	,	,	,
cord-345088-krb1eidw	189	11	10.4	10.4	CD
cord-345088-krb1eidw	189	12	%	%	NN
cord-345088-krb1eidw	189	13	(	(	-LRB-
cord-345088-krb1eidw	189	14	15.9	15.9	CD
cord-345088-krb1eidw	189	15	-5.5	-5.5	NNP
cord-345088-krb1eidw	189	16	)	)	-RRB-
cord-345088-krb1eidw	189	17	of	of	IN
cord-345088-krb1eidw	189	18	cells	cell	NNS
cord-345088-krb1eidw	189	19	expressing	express	VBG
cord-345088-krb1eidw	189	20	the	the	DT
cord-345088-krb1eidw	189	21	wt	wt	NN
cord-345088-krb1eidw	189	22	S	S	NNP
cord-345088-krb1eidw	189	23	protein	protein	NN
cord-345088-krb1eidw	189	24	(	(	-LRB-
cord-345088-krb1eidw	189	25	panel	panel	NN
cord-345088-krb1eidw	189	26	D	D	NNP
cord-345088-krb1eidw	189	27	)	)	-RRB-
cord-345088-krb1eidw	189	28	and	and	CC
cord-345088-krb1eidw	189	29	2.3	2.3	CD
cord-345088-krb1eidw	189	30	%	%	NN
cord-345088-krb1eidw	189	31	(	(	-LRB-
cord-345088-krb1eidw	189	32	7.8	7.8	CD
cord-345088-krb1eidw	189	33	-5.5	-5.5	ADD
cord-345088-krb1eidw	189	34	)	)	-RRB-
cord-345088-krb1eidw	189	35	of	of	IN
cord-345088-krb1eidw	189	36	cells	cell	NNS
cord-345088-krb1eidw	189	37	expressing	express	VBG
cord-345088-krb1eidw	189	38	the	the	DT
cord-345088-krb1eidw	189	39	ts	ts	NNP
cord-345088-krb1eidw	189	40	mutant	mutant	NN
cord-345088-krb1eidw	189	41	S	S	NNP
cord-345088-krb1eidw	189	42	protein	protein	NN
cord-345088-krb1eidw	189	43	(	(	-LRB-
cord-345088-krb1eidw	189	44	panel	panel	NN
cord-345088-krb1eidw	189	45	E	E	NNP
cord-345088-krb1eidw	189	46	)	)	-RRB-
cord-345088-krb1eidw	189	47	exhibited	exhibit	VBN
cord-345088-krb1eidw	189	48	surface	surface	NN
cord-345088-krb1eidw	189	49	staining	staining	NN
cord-345088-krb1eidw	189	50	.	.	.
cord-345088-krb1eidw	190	1	After	after	IN
cord-345088-krb1eidw	190	2	permeabilizing	permeabilize	VBG
cord-345088-krb1eidw	190	3	with	with	IN
cord-345088-krb1eidw	190	4	0.1	0.1	CD
cord-345088-krb1eidw	190	5	%	%	NN
cord-345088-krb1eidw	190	6	saponin	saponin	NN
cord-345088-krb1eidw	190	7	,	,	,
cord-345088-krb1eidw	190	8	9.3	9.3	CD
cord-345088-krb1eidw	190	9	%	%	NN
cord-345088-krb1eidw	190	10	(	(	-LRB-
cord-345088-krb1eidw	190	11	10	10	CD
cord-345088-krb1eidw	190	12	-0.7	-0.7	NN
cord-345088-krb1eidw	190	13	)	)	-RRB-
cord-345088-krb1eidw	190	14	of	of	IN
cord-345088-krb1eidw	190	15	cells	cell	NNS
cord-345088-krb1eidw	190	16	expressing	express	VBG
cord-345088-krb1eidw	190	17	the	the	DT
cord-345088-krb1eidw	190	18	wt	wt	NN
cord-345088-krb1eidw	190	19	S	S	NNP
cord-345088-krb1eidw	190	20	protein	protein	NN
cord-345088-krb1eidw	190	21	(	(	-LRB-
cord-345088-krb1eidw	190	22	panel	panel	NN
cord-345088-krb1eidw	190	23	I	I	NNP
cord-345088-krb1eidw	190	24	)	)	-RRB-
cord-345088-krb1eidw	190	25	and	and	CC
cord-345088-krb1eidw	190	26	9.7	9.7	CD
cord-345088-krb1eidw	190	27	%	%	NN
cord-345088-krb1eidw	190	28	(	(	-LRB-
cord-345088-krb1eidw	190	29	10.4	10.4	CD
cord-345088-krb1eidw	190	30	-0.7	-0.7	NN
cord-345088-krb1eidw	190	31	)	)	-RRB-
cord-345088-krb1eidw	190	32	of	of	IN
cord-345088-krb1eidw	190	33	cells	cell	NNS
cord-345088-krb1eidw	190	34	expressing	express	VBG
cord-345088-krb1eidw	190	35	the	the	DT
cord-345088-krb1eidw	190	36	ts	ts	NNP
cord-345088-krb1eidw	190	37	mutant	mutant	NN
cord-345088-krb1eidw	190	38	S	S	NNP
cord-345088-krb1eidw	190	39	protein	protein	NN
cord-345088-krb1eidw	190	40	(	(	-LRB-
cord-345088-krb1eidw	190	41	panel	panel	NN
cord-345088-krb1eidw	190	42	J	J	NNP
cord-345088-krb1eidw	190	43	)	)	-RRB-
cord-345088-krb1eidw	190	44	showed	show	VBD
cord-345088-krb1eidw	190	45	positive	positive	JJ
cord-345088-krb1eidw	190	46	staining	staining	NN
cord-345088-krb1eidw	190	47	.	.	.
cord-345088-krb1eidw	191	1	These	these	DT
cord-345088-krb1eidw	191	2	results	result	NNS
cord-345088-krb1eidw	191	3	confirm	confirm	VBP
cord-345088-krb1eidw	191	4	that	that	IN
cord-345088-krb1eidw	191	5	the	the	DT
cord-345088-krb1eidw	191	6	ts	ts	NNP
cord-345088-krb1eidw	191	7	mutant	mutant	NN
cord-345088-krb1eidw	191	8	S	S	NNP
cord-345088-krb1eidw	191	9	protein	protein	NN
cord-345088-krb1eidw	191	10	could	could	MD
cord-345088-krb1eidw	191	11	not	not	RB
cord-345088-krb1eidw	191	12	be	be	VB
cord-345088-krb1eidw	191	13	efficiently	efficiently	RB
cord-345088-krb1eidw	191	14	translocated	translocate	VBN
cord-345088-krb1eidw	191	15	to	to	IN
cord-345088-krb1eidw	191	16	the	the	DT
cord-345088-krb1eidw	191	17	cell	cell	NN
cord-345088-krb1eidw	191	18	surface	surface	NN
cord-345088-krb1eidw	191	19	at	at	IN
cord-345088-krb1eidw	191	20	the	the	DT
cord-345088-krb1eidw	191	21	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	191	22	temperature	temperature	NN
cord-345088-krb1eidw	191	23	.	.	.
cord-345088-krb1eidw	192	1	To	to	TO
cord-345088-krb1eidw	192	2	investigate	investigate	VB
cord-345088-krb1eidw	192	3	whether	whether	IN
cord-345088-krb1eidw	192	4	the	the	DT
cord-345088-krb1eidw	192	5	S	S	NNP
cord-345088-krb1eidw	192	6	protein	protein	NN
cord-345088-krb1eidw	192	7	of	of	IN
cord-345088-krb1eidw	192	8	ts	ts	NNP
cord-345088-krb1eidw	192	9	mutant	mutant	NN
cord-345088-krb1eidw	192	10	was	be	VBD
cord-345088-krb1eidw	192	11	assembled	assemble	VBN
cord-345088-krb1eidw	192	12	into	into	IN
cord-345088-krb1eidw	192	13	virion	virion	NN
cord-345088-krb1eidw	192	14	or	or	CC
cord-345088-krb1eidw	192	15	not	not	RB
cord-345088-krb1eidw	192	16	,	,	,
cord-345088-krb1eidw	192	17	ts291602	ts291602	NNP
cord-345088-krb1eidw	192	18	and	and	CC
cord-345088-krb1eidw	192	19	rev-1-infected	rev-1-infected	JJ
cord-345088-krb1eidw	192	20	cells	cell	NNS
cord-345088-krb1eidw	192	21	were	be	VBD
cord-345088-krb1eidw	192	22	radiolabeled	radiolabele	VBN
cord-345088-krb1eidw	192	23	and	and	CC
cord-345088-krb1eidw	192	24	the	the	DT
cord-345088-krb1eidw	192	25	virus	virus	NN
cord-345088-krb1eidw	192	26	particles	particle	NNS
cord-345088-krb1eidw	192	27	were	be	VBD
cord-345088-krb1eidw	192	28	purified	purify	VBN
cord-345088-krb1eidw	192	29	through	through	IN
cord-345088-krb1eidw	192	30	sucrose	sucrose	NN
cord-345088-krb1eidw	192	31	gradients	gradient	NNS
cord-345088-krb1eidw	192	32	twice	twice	RB
cord-345088-krb1eidw	192	33	.	.	.
cord-345088-krb1eidw	193	1	Immunoprecipitation	immunoprecipitation	NN
cord-345088-krb1eidw	193	2	of	of	IN
cord-345088-krb1eidw	193	3	the	the	DT
cord-345088-krb1eidw	193	4	purified	purify	VBN
cord-345088-krb1eidw	193	5	virions	virion	NNS
cord-345088-krb1eidw	193	6	using	use	VBG
cord-345088-krb1eidw	193	7	anti	anti	JJ
cord-345088-krb1eidw	193	8	-	-	JJ
cord-345088-krb1eidw	193	9	IBV	IBV	NNP
cord-345088-krb1eidw	193	10	antibodies	antibody	NNS
cord-345088-krb1eidw	193	11	showed	show	VBD
cord-345088-krb1eidw	193	12	that	that	IN
cord-345088-krb1eidw	193	13	the	the	DT
cord-345088-krb1eidw	193	14	S	S	NNP
cord-345088-krb1eidw	193	15	protein	protein	NN
cord-345088-krb1eidw	193	16	of	of	IN
cord-345088-krb1eidw	193	17	ts291602	ts291602	NNP
cord-345088-krb1eidw	193	18	was	be	VBD
cord-345088-krb1eidw	193	19	not	not	RB
cord-345088-krb1eidw	193	20	detected	detect	VBN
cord-345088-krb1eidw	193	21	at	at	IN
cord-345088-krb1eidw	193	22	40	40	CD
cord-345088-krb1eidw	193	23	jC	jC	NNS
cord-345088-krb1eidw	194	1	(	(	-LRB-
cord-345088-krb1eidw	194	2	Fig	Fig	NNP
cord-345088-krb1eidw	194	3	.	.	NNP
cord-345088-krb1eidw	194	4	6	6	CD
cord-345088-krb1eidw	194	5	,	,	,
cord-345088-krb1eidw	194	6	lane	lane	NN
cord-345088-krb1eidw	194	7	2	2	CD
cord-345088-krb1eidw	194	8	)	)	-RRB-
cord-345088-krb1eidw	194	9	,	,	,
cord-345088-krb1eidw	194	10	but	but	CC
cord-345088-krb1eidw	194	11	was	be	VBD
cord-345088-krb1eidw	194	12	observed	observe	VBN
cord-345088-krb1eidw	194	13	at	at	IN
cord-345088-krb1eidw	194	14	32	32	CD
cord-345088-krb1eidw	194	15	jC	jC	NNS
cord-345088-krb1eidw	195	1	(	(	-LRB-
cord-345088-krb1eidw	195	2	Fig	Fig	NNP
cord-345088-krb1eidw	195	3	.	.	NNP
cord-345088-krb1eidw	195	4	6	6	CD
cord-345088-krb1eidw	195	5	,	,	,
cord-345088-krb1eidw	195	6	lane	lane	NN
cord-345088-krb1eidw	195	7	1	1	CD
cord-345088-krb1eidw	195	8	)	)	-RRB-
cord-345088-krb1eidw	195	9	.	.	.
cord-345088-krb1eidw	196	1	The	the	DT
cord-345088-krb1eidw	196	2	S	S	NNP
cord-345088-krb1eidw	196	3	protein	protein	NN
cord-345088-krb1eidw	196	4	was	be	VBD
cord-345088-krb1eidw	196	5	detected	detect	VBN
cord-345088-krb1eidw	196	6	at	at	IN
cord-345088-krb1eidw	196	7	both	both	DT
cord-345088-krb1eidw	196	8	temperatures	temperature	NNS
cord-345088-krb1eidw	196	9	for	for	IN
cord-345088-krb1eidw	197	1	rev-1	rev-1	LS
cord-345088-krb1eidw	197	2	(	(	-LRB-
cord-345088-krb1eidw	197	3	Fig	Fig	NNP
cord-345088-krb1eidw	197	4	.	.	NNP
cord-345088-krb1eidw	197	5	6	6	CD
cord-345088-krb1eidw	197	6	,	,	,
cord-345088-krb1eidw	197	7	 	 	_SP
cord-345088-krb1eidw	197	8	lanes	lane	NNS
cord-345088-krb1eidw	197	9	3	3	CD
cord-345088-krb1eidw	197	10	and	and	CC
cord-345088-krb1eidw	197	11	4	4	CD
cord-345088-krb1eidw	197	12	)	)	-RRB-
cord-345088-krb1eidw	197	13	.	.	.
cord-345088-krb1eidw	198	1	These	these	DT
cord-345088-krb1eidw	198	2	results	result	NNS
cord-345088-krb1eidw	198	3	render	render	VBP
cord-345088-krb1eidw	198	4	support	support	NN
cord-345088-krb1eidw	198	5	that	that	IN
cord-345088-krb1eidw	198	6	the	the	DT
cord-345088-krb1eidw	198	7	spike	spike	NN
cord-345088-krb1eidw	198	8	protein	protein	NN
cord-345088-krb1eidw	198	9	of	of	IN
cord-345088-krb1eidw	198	10	the	the	DT
cord-345088-krb1eidw	198	11	ts	ts	NNP
cord-345088-krb1eidw	198	12	mutant	mutant	NN
cord-345088-krb1eidw	198	13	may	may	MD
cord-345088-krb1eidw	198	14	not	not	RB
cord-345088-krb1eidw	198	15	be	be	VB
cord-345088-krb1eidw	198	16	assembled	assemble	VBN
cord-345088-krb1eidw	198	17	into	into	IN
cord-345088-krb1eidw	198	18	virus	virus	NN
cord-345088-krb1eidw	198	19	particles	particle	NNS
cord-345088-krb1eidw	198	20	at	at	IN
cord-345088-krb1eidw	198	21	40	40	CD
cord-345088-krb1eidw	198	22	jC.	jc.	NN
cord-345088-krb1eidw	198	23	Coronavirus	Coronavirus	NNP
cord-345088-krb1eidw	198	24	S	S	NNP
cord-345088-krb1eidw	198	25	protein	protein	NN
cord-345088-krb1eidw	198	26	is	be	VBZ
cord-345088-krb1eidw	198	27	responsible	responsible	JJ
cord-345088-krb1eidw	198	28	for	for	IN
cord-345088-krb1eidw	198	29	receptor	receptor	NN
cord-345088-krb1eidw	198	30	binding	binding	NN
cord-345088-krb1eidw	198	31	and	and	CC
cord-345088-krb1eidw	198	32	membrane	membrane	NN
cord-345088-krb1eidw	198	33	fusion	fusion	NN
cord-345088-krb1eidw	198	34	.	.	.
cord-345088-krb1eidw	199	1	It	-PRON-	PRP
cord-345088-krb1eidw	199	2	also	also	RB
cord-345088-krb1eidw	199	3	induces	induce	VBZ
cord-345088-krb1eidw	199	4	neutralizing	neutralize	VBG
cord-345088-krb1eidw	199	5	antibodies	antibody	NNS
cord-345088-krb1eidw	199	6	and	and	CC
cord-345088-krb1eidw	199	7	bears	bear	VBZ
cord-345088-krb1eidw	199	8	determinants	determinant	NNS
cord-345088-krb1eidw	199	9	for	for	IN
cord-345088-krb1eidw	199	10	virulence	virulence	NN
cord-345088-krb1eidw	199	11	.	.	.
cord-345088-krb1eidw	200	1	A	a	DT
cord-345088-krb1eidw	200	2	considerable	considerable	JJ
cord-345088-krb1eidw	200	3	diversity	diversity	NN
cord-345088-krb1eidw	200	4	in	in	IN
cord-345088-krb1eidw	200	5	S	s	NN
cord-345088-krb1eidw	200	6	protein	protein	NN
cord-345088-krb1eidw	200	7	among	among	IN
cord-345088-krb1eidw	200	8	coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	200	9	exists	exist	VBZ
cord-345088-krb1eidw	200	10	,	,	,
cord-345088-krb1eidw	200	11	which	which	WDT
cord-345088-krb1eidw	200	12	contributes	contribute	VBZ
cord-345088-krb1eidw	200	13	to	to	TO
cord-345088-krb1eidw	200	14	host	host	VB
cord-345088-krb1eidw	200	15	specificity	specificity	NN
cord-345088-krb1eidw	200	16	,	,	,
cord-345088-krb1eidw	200	17	cell	cell	NN
cord-345088-krb1eidw	200	18	and	and	CC
cord-345088-krb1eidw	200	19	organ	organ	NN
cord-345088-krb1eidw	200	20	tropisms	tropism	NNS
cord-345088-krb1eidw	200	21	,	,	,
cord-345088-krb1eidw	200	22	and	and	CC
cord-345088-krb1eidw	200	23	pathogenesis	pathogenesis	NN
cord-345088-krb1eidw	200	24	.	.	.
cord-345088-krb1eidw	201	1	Characterization	characterization	NN
cord-345088-krb1eidw	201	2	of	of	IN
cord-345088-krb1eidw	201	3	mutants	mutant	NNS
cord-345088-krb1eidw	201	4	with	with	IN
cord-345088-krb1eidw	201	5	point	point	NN
cord-345088-krb1eidw	201	6	or	or	CC
cord-345088-krb1eidw	201	7	deletion	deletion	NN
cord-345088-krb1eidw	201	8	mutations	mutation	NNS
cord-345088-krb1eidw	201	9	in	in	IN
cord-345088-krb1eidw	201	10	the	the	DT
cord-345088-krb1eidw	201	11	S	S	NNP
cord-345088-krb1eidw	201	12	protein	protein	NN
cord-345088-krb1eidw	201	13	has	have	VBZ
cord-345088-krb1eidw	201	14	helped	help	VBN
cord-345088-krb1eidw	201	15	to	to	TO
cord-345088-krb1eidw	201	16	establish	establish	VB
cord-345088-krb1eidw	201	17	links	link	NNS
cord-345088-krb1eidw	201	18	between	between	IN
cord-345088-krb1eidw	201	19	the	the	DT
cord-345088-krb1eidw	201	20	variations	variation	NNS
cord-345088-krb1eidw	201	21	and	and	CC
cord-345088-krb1eidw	201	22	the	the	DT
cord-345088-krb1eidw	201	23	functions	function	NNS
cord-345088-krb1eidw	201	24	or	or	CC
cord-345088-krb1eidw	201	25	altered	alter	VBN
cord-345088-krb1eidw	201	26	antigenicity	antigenicity	NN
cord-345088-krb1eidw	201	27	and	and	CC
cord-345088-krb1eidw	201	28	virulence	virulence	NN
cord-345088-krb1eidw	201	29	of	of	IN
cord-345088-krb1eidw	201	30	viruses	virus	NNS
cord-345088-krb1eidw	201	31	.	.	.
cord-345088-krb1eidw	202	1	Through	through	IN
cord-345088-krb1eidw	202	2	sequence	sequence	NN
cord-345088-krb1eidw	202	3	analysis	analysis	NN
cord-345088-krb1eidw	202	4	of	of	IN
cord-345088-krb1eidw	202	5	wt	wt	NN
cord-345088-krb1eidw	202	6	virus	virus	NN
cord-345088-krb1eidw	202	7	,	,	,
cord-345088-krb1eidw	202	8	two	two	CD
cord-345088-krb1eidw	202	9	ts	ts	NNP
cord-345088-krb1eidw	202	10	mutants	mutant	NNS
cord-345088-krb1eidw	202	11	,	,	,
cord-345088-krb1eidw	202	12	a	a	DT
cord-345088-krb1eidw	202	13	revertant	revertant	NN
cord-345088-krb1eidw	202	14	and	and	CC
cord-345088-krb1eidw	202	15	different	different	JJ
cord-345088-krb1eidw	202	16	passages	passage	NNS
cord-345088-krb1eidw	202	17	of	of	IN
cord-345088-krb1eidw	202	18	a	a	DT
cord-345088-krb1eidw	202	19	cold	cold	NN
cord-345088-krb1eidw	202	20	-	-	HYPH
cord-345088-krb1eidw	202	21	adapted	adapt	VBN
cord-345088-krb1eidw	202	22	IBV	IBV	NNP
cord-345088-krb1eidw	202	23	,	,	,
cord-345088-krb1eidw	202	24	data	datum	NNS
cord-345088-krb1eidw	202	25	presented	present	VBN
cord-345088-krb1eidw	202	26	in	in	IN
cord-345088-krb1eidw	202	27	this	this	DT
cord-345088-krb1eidw	202	28	study	study	NN
cord-345088-krb1eidw	202	29	not	not	RB
cord-345088-krb1eidw	202	30	only	only	RB
cord-345088-krb1eidw	202	31	mapped	map	VBD
cord-345088-krb1eidw	202	32	the	the	DT
cord-345088-krb1eidw	202	33	defects	defect	NNS
cord-345088-krb1eidw	202	34	of	of	IN
cord-345088-krb1eidw	202	35	the	the	DT
cord-345088-krb1eidw	202	36	ts	ts	NNP
cord-345088-krb1eidw	202	37	mutants	mutant	NNS
cord-345088-krb1eidw	202	38	to	to	IN
cord-345088-krb1eidw	202	39	the	the	DT
cord-345088-krb1eidw	202	40	S	S	NNP
cord-345088-krb1eidw	202	41	gene	gene	NN
cord-345088-krb1eidw	202	42	,	,	,
cord-345088-krb1eidw	202	43	but	but	CC
cord-345088-krb1eidw	202	44	also	also	RB
cord-345088-krb1eidw	202	45	revealed	reveal	VBD
cord-345088-krb1eidw	202	46	the	the	DT
cord-345088-krb1eidw	202	47	molecular	molecular	JJ
cord-345088-krb1eidw	202	48	events	event	NNS
cord-345088-krb1eidw	202	49	occurred	occur	VBD
cord-345088-krb1eidw	202	50	during	during	IN
cord-345088-krb1eidw	202	51	evolution	evolution	NN
cord-345088-krb1eidw	202	52	of	of	IN
cord-345088-krb1eidw	202	53	the	the	DT
cord-345088-krb1eidw	202	54	IBV	IBV	NNP
cord-345088-krb1eidw	202	55	S	S	NNP
cord-345088-krb1eidw	202	56	gene	gene	NN
cord-345088-krb1eidw	202	57	.	.	.
cord-345088-krb1eidw	203	1	Compared	compare	VBN
cord-345088-krb1eidw	203	2	with	with	IN
cord-345088-krb1eidw	203	3	the	the	DT
cord-345088-krb1eidw	203	4	parental	parental	JJ
cord-345088-krb1eidw	203	5	wt	wt	NN
cord-345088-krb1eidw	203	6	virus	virus	NN
cord-345088-krb1eidw	203	7	,	,	,
cord-345088-krb1eidw	203	8	mutations	mutation	NNS
cord-345088-krb1eidw	203	9	that	that	WDT
cord-345088-krb1eidw	203	10	are	be	VBP
cord-345088-krb1eidw	203	11	identical	identical	JJ
cord-345088-krb1eidw	203	12	in	in	IN
cord-345088-krb1eidw	203	13	the	the	DT
cord-345088-krb1eidw	203	14	two	two	CD
cord-345088-krb1eidw	203	15	ts	ts	NNP
cord-345088-krb1eidw	203	16	mutants	mutant	NNS
cord-345088-krb1eidw	203	17	and	and	CC
cord-345088-krb1eidw	203	18	the	the	DT
cord-345088-krb1eidw	203	19	revertant	revertant	NN
cord-345088-krb1eidw	203	20	might	may	MD
cord-345088-krb1eidw	203	21	be	be	VB
cord-345088-krb1eidw	203	22	associated	associate	VBN
cord-345088-krb1eidw	203	23	with	with	IN
cord-345088-krb1eidw	203	24	the	the	DT
cord-345088-krb1eidw	203	25	cold	cold	JJ
cord-345088-krb1eidw	203	26	-	-	HYPH
cord-345088-krb1eidw	203	27	adaptation	adaptation	NN
cord-345088-krb1eidw	203	28	phenotype	phenotype	NN
cord-345088-krb1eidw	203	29	,	,	,
cord-345088-krb1eidw	203	30	while	while	IN
cord-345088-krb1eidw	203	31	those	those	DT
cord-345088-krb1eidw	203	32	identical	identical	JJ
cord-345088-krb1eidw	203	33	only	only	RB
cord-345088-krb1eidw	203	34	in	in	IN
cord-345088-krb1eidw	203	35	the	the	DT
cord-345088-krb1eidw	203	36	two	two	CD
cord-345088-krb1eidw	203	37	mutants	mutant	NNS
cord-345088-krb1eidw	203	38	but	but	CC
cord-345088-krb1eidw	203	39	different	different	JJ
cord-345088-krb1eidw	203	40	from	from	IN
cord-345088-krb1eidw	203	41	the	the	DT
cord-345088-krb1eidw	203	42	revertant	revertant	NN
cord-345088-krb1eidw	203	43	and	and	CC
cord-345088-krb1eidw	203	44	wt	wt	NN
cord-345088-krb1eidw	203	45	virus	virus	NN
cord-345088-krb1eidw	203	46	were	be	VBD
cord-345088-krb1eidw	203	47	considered	consider	VBN
cord-345088-krb1eidw	203	48	to	to	TO
cord-345088-krb1eidw	203	49	be	be	VB
cord-345088-krb1eidw	203	50	linked	link	VBN
cord-345088-krb1eidw	203	51	to	to	IN
cord-345088-krb1eidw	203	52	the	the	DT
cord-345088-krb1eidw	203	53	ts	ts	NNP
cord-345088-krb1eidw	203	54	phenotype	phenotype	NN
cord-345088-krb1eidw	203	55	.	.	.
cord-345088-krb1eidw	204	1	According	accord	VBG
cord-345088-krb1eidw	204	2	to	to	IN
cord-345088-krb1eidw	204	3	this	this	DT
cord-345088-krb1eidw	204	4	criterion	criterion	NN
cord-345088-krb1eidw	204	5	,	,	,
cord-345088-krb1eidw	204	6	the	the	DT
cord-345088-krb1eidw	204	7	Q	Q	NNP
cord-345088-krb1eidw	204	8	294	294	CD
cord-345088-krb1eidw	204	9	-to	-to	NNPS
cord-345088-krb1eidw	204	10	-	-	:
cord-345088-krb1eidw	204	11	L	l	NN
cord-345088-krb1eidw	204	12	294	294	CD
cord-345088-krb1eidw	204	13	mutation	mutation	NN
cord-345088-krb1eidw	204	14	in	in	IN
cord-345088-krb1eidw	204	15	the	the	DT
cord-345088-krb1eidw	204	16	S	S	NNP
cord-345088-krb1eidw	204	17	protein	protein	NN
cord-345088-krb1eidw	204	18	that	that	WDT
cord-345088-krb1eidw	204	19	exists	exist	VBZ
cord-345088-krb1eidw	204	20	in	in	IN
cord-345088-krb1eidw	204	21	the	the	DT
cord-345088-krb1eidw	204	22	two	two	CD
cord-345088-krb1eidw	204	23	ts	ts	NNP
cord-345088-krb1eidw	204	24	mutants	mutant	NNS
cord-345088-krb1eidw	204	25	,	,	,
cord-345088-krb1eidw	204	26	and	and	CC
cord-345088-krb1eidw	204	27	interestingly	interestingly	RB
cord-345088-krb1eidw	204	28	,	,	,
cord-345088-krb1eidw	204	29	occurred	occur	VBD
cord-345088-krb1eidw	204	30	at	at	IN
cord-345088-krb1eidw	204	31	a	a	DT
cord-345088-krb1eidw	204	32	highly	highly	RB
cord-345088-krb1eidw	204	33	conserved	conserved	JJ
cord-345088-krb1eidw	204	34	domain	domain	NN
cord-345088-krb1eidw	204	35	among	among	IN
cord-345088-krb1eidw	204	36	IBV	IBV	NNP
cord-345088-krb1eidw	204	37	viruses	virus	NNS
cord-345088-krb1eidw	204	38	immediately	immediately	RB
cord-345088-krb1eidw	204	39	downstream	downstream	VBP
cord-345088-krb1eidw	204	40	a	a	DT
cord-345088-krb1eidw	204	41	variable	variable	JJ
cord-345088-krb1eidw	204	42	domain	domain	NN
cord-345088-krb1eidw	204	43	in	in	IN
cord-345088-krb1eidw	204	44	the	the	DT
cord-345088-krb1eidw	204	45	S1	S1	NNP
cord-345088-krb1eidw	204	46	subunit	subunit	NN
cord-345088-krb1eidw	204	47	may	may	MD
cord-345088-krb1eidw	204	48	be	be	VB
cord-345088-krb1eidw	204	49	responsible	responsible	JJ
cord-345088-krb1eidw	204	50	for	for	IN
cord-345088-krb1eidw	204	51	the	the	DT
cord-345088-krb1eidw	204	52	ts	ts	NNP
cord-345088-krb1eidw	204	53	phenotype	phenotype	NN
cord-345088-krb1eidw	204	54	.	.	.
cord-345088-krb1eidw	205	1	The	the	DT
cord-345088-krb1eidw	205	2	mutants	mutant	NNS
cord-345088-krb1eidw	205	3	with	with	IN
cord-345088-krb1eidw	205	4	this	this	DT
cord-345088-krb1eidw	205	5	mutation	mutation	NN
cord-345088-krb1eidw	205	6	accumulated	accumulate	VBN
cord-345088-krb1eidw	205	7	and	and	CC
cord-345088-krb1eidw	205	8	became	become	VBD
cord-345088-krb1eidw	205	9	dominant	dominant	JJ
cord-345088-krb1eidw	205	10	under	under	IN
cord-345088-krb1eidw	205	11	selective	selective	JJ
cord-345088-krb1eidw	205	12	pressures	pressure	NNS
cord-345088-krb1eidw	205	13	,	,	,
cord-345088-krb1eidw	205	14	in	in	IN
cord-345088-krb1eidw	205	15	this	this	DT
cord-345088-krb1eidw	205	16	case	case	NN
cord-345088-krb1eidw	205	17	,	,	,
cord-345088-krb1eidw	205	18	changes	change	NNS
cord-345088-krb1eidw	205	19	in	in	IN
cord-345088-krb1eidw	205	20	the	the	DT
cord-345088-krb1eidw	205	21	hosts	host	NNS
cord-345088-krb1eidw	205	22	and	and	CC
cord-345088-krb1eidw	205	23	temperatures	temperature	NNS
cord-345088-krb1eidw	205	24	.	.	.
cord-345088-krb1eidw	206	1	The	the	DT
cord-345088-krb1eidw	206	2	I	I	NNP
cord-345088-krb1eidw	206	3	769	769	CD
cord-345088-krb1eidw	206	4	-M	-M	:
cord-345088-krb1eidw	206	5	769	769	CD
cord-345088-krb1eidw	206	6	mutation	mutation	NN
cord-345088-krb1eidw	206	7	emerged	emerge	VBD
cord-345088-krb1eidw	206	8	earlier	early	RBR
cord-345088-krb1eidw	206	9	than	than	IN
cord-345088-krb1eidw	206	10	the	the	DT
cord-345088-krb1eidw	206	11	Q	Q	NNP
cord-345088-krb1eidw	206	12	294	294	CD
cord-345088-krb1eidw	206	13	-L	-l	SYM
cord-345088-krb1eidw	206	14	294	294	CD
cord-345088-krb1eidw	206	15	mutation	mutation	NN
cord-345088-krb1eidw	206	16	,	,	,
cord-345088-krb1eidw	206	17	which	which	WDT
cord-345088-krb1eidw	206	18	among	among	IN
cord-345088-krb1eidw	206	19	variations	variation	NNS
cord-345088-krb1eidw	206	20	in	in	IN
cord-345088-krb1eidw	206	21	other	other	JJ
cord-345088-krb1eidw	206	22	gene	gene	NN
cord-345088-krb1eidw	206	23	products	product	NNS
cord-345088-krb1eidw	206	24	may	may	MD
cord-345088-krb1eidw	206	25	be	be	VB
cord-345088-krb1eidw	206	26	associated	associate	VBN
cord-345088-krb1eidw	206	27	with	with	IN
cord-345088-krb1eidw	206	28	cold	cold	JJ
cord-345088-krb1eidw	206	29	-	-	HYPH
cord-345088-krb1eidw	206	30	adaptation	adaptation	NN
cord-345088-krb1eidw	206	31	,	,	,
cord-345088-krb1eidw	206	32	suggesting	suggest	VBG
cord-345088-krb1eidw	206	33	potential	potential	JJ
cord-345088-krb1eidw	206	34	segregation	segregation	NN
cord-345088-krb1eidw	206	35	of	of	IN
cord-345088-krb1eidw	206	36	mutations	mutation	NNS
cord-345088-krb1eidw	206	37	responsible	responsible	JJ
cord-345088-krb1eidw	206	38	for	for	IN
cord-345088-krb1eidw	206	39	either	either	DT
cord-345088-krb1eidw	206	40	cold	cold	JJ
cord-345088-krb1eidw	206	41	-	-	HYPH
cord-345088-krb1eidw	206	42	adaptation	adaptation	NN
cord-345088-krb1eidw	206	43	or	or	CC
cord-345088-krb1eidw	206	44	ts	ts	NN
cord-345088-krb1eidw	206	45	phenotypes	phenotype	NNS
cord-345088-krb1eidw	206	46	.	.	.
cord-345088-krb1eidw	207	1	Further	further	JJ
cord-345088-krb1eidw	207	2	confirmation	confirmation	NN
cord-345088-krb1eidw	207	3	of	of	IN
cord-345088-krb1eidw	207	4	the	the	DT
cord-345088-krb1eidw	207	5	possibility	possibility	NN
cord-345088-krb1eidw	207	6	that	that	IN
cord-345088-krb1eidw	207	7	the	the	DT
cord-345088-krb1eidw	207	8	Q	Q	NNP
cord-345088-krb1eidw	207	9	294	294	CD
cord-345088-krb1eidw	207	10	-to	-to	NNPS
cord-345088-krb1eidw	207	11	-	-	:
cord-345088-krb1eidw	207	12	L	l	NN
cord-345088-krb1eidw	207	13	294	294	CD
cord-345088-krb1eidw	207	14	mutation	mutation	NN
cord-345088-krb1eidw	207	15	may	may	MD
cord-345088-krb1eidw	207	16	cause	cause	VB
cord-345088-krb1eidw	207	17	the	the	DT
cord-345088-krb1eidw	207	18	ts	ts	NNP
cord-345088-krb1eidw	207	19	phenotypic	phenotypic	NN
cord-345088-krb1eidw	207	20	changes	change	NNS
cord-345088-krb1eidw	207	21	is	be	VBZ
cord-345088-krb1eidw	207	22	currently	currently	RB
cord-345088-krb1eidw	207	23	being	be	VBG
cord-345088-krb1eidw	207	24	carried	carry	VBN
cord-345088-krb1eidw	207	25	out	out	RP
cord-345088-krb1eidw	207	26	by	by	IN
cord-345088-krb1eidw	207	27	introducing	introduce	VBG
cord-345088-krb1eidw	207	28	the	the	DT
cord-345088-krb1eidw	207	29	mutation	mutation	NN
cord-345088-krb1eidw	207	30	to	to	IN
cord-345088-krb1eidw	207	31	an	an	DT
cord-345088-krb1eidw	207	32	infectious	infectious	JJ
cord-345088-krb1eidw	207	33	IBV	IBV	NNP
cord-345088-krb1eidw	207	34	clones	clone	NNS
cord-345088-krb1eidw	207	35	and	and	CC
cord-345088-krb1eidw	207	36	isolation	isolation	NN
cord-345088-krb1eidw	207	37	of	of	IN
cord-345088-krb1eidw	207	38	a	a	DT
cord-345088-krb1eidw	207	39	recombinant	recombinant	JJ
cord-345088-krb1eidw	207	40	virus	virus	NN
cord-345088-krb1eidw	207	41	containing	contain	VBG
cord-345088-krb1eidw	207	42	this	this	DT
cord-345088-krb1eidw	207	43	mutation	mutation	NN
cord-345088-krb1eidw	207	44	only	only	RB
cord-345088-krb1eidw	207	45	.	.	.
cord-345088-krb1eidw	208	1	The	the	DT
cord-345088-krb1eidw	208	2	S	S	NNP
cord-345088-krb1eidw	208	3	protein	protein	NN
cord-345088-krb1eidw	208	4	is	be	VBZ
cord-345088-krb1eidw	208	5	co	co	NN
cord-345088-krb1eidw	208	6	-	-	NN
cord-345088-krb1eidw	208	7	translationally	translationally	RB
cord-345088-krb1eidw	208	8	N	n	NN
cord-345088-krb1eidw	208	9	-	-	HYPH
cord-345088-krb1eidw	208	10	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	208	11	in	in	IN
cord-345088-krb1eidw	208	12	the	the	DT
cord-345088-krb1eidw	208	13	ER	ER	NNP
cord-345088-krb1eidw	208	14	,	,	,
cord-345088-krb1eidw	208	15	oligomerized	oligomerize	VBN
cord-345088-krb1eidw	208	16	(	(	-LRB-
cord-345088-krb1eidw	208	17	Luytjes	Luytjes	NNP
cord-345088-krb1eidw	208	18	et	et	NNP
cord-345088-krb1eidw	208	19	al	al	NNP
cord-345088-krb1eidw	208	20	.	.	NNP
cord-345088-krb1eidw	208	21	,	,	,
cord-345088-krb1eidw	208	22	1997	1997	CD
cord-345088-krb1eidw	208	23	)	)	-RRB-
cord-345088-krb1eidw	208	24	if	if	IN
cord-345088-krb1eidw	208	25	folded	fold	VBN
cord-345088-krb1eidw	208	26	properly	properly	RB
cord-345088-krb1eidw	208	27	,	,	,
cord-345088-krb1eidw	208	28	and	and	CC
cord-345088-krb1eidw	208	29	associated	associate	VBN
cord-345088-krb1eidw	208	30	with	with	IN
cord-345088-krb1eidw	208	31	the	the	DT
cord-345088-krb1eidw	208	32	M	M	NNP
cord-345088-krb1eidw	208	33	protein	protein	NN
cord-345088-krb1eidw	208	34	in	in	IN
cord-345088-krb1eidw	208	35	pre	pre	JJ
cord-345088-krb1eidw	208	36	-	-	JJ
cord-345088-krb1eidw	208	37	Golgi	golgi	JJ
cord-345088-krb1eidw	208	38	membrane	membrane	NN
cord-345088-krb1eidw	208	39	(	(	-LRB-
cord-345088-krb1eidw	208	40	Opstelten	Opstelten	NNP
cord-345088-krb1eidw	208	41	et	et	NNP
cord-345088-krb1eidw	208	42	al	al	NNP
cord-345088-krb1eidw	208	43	.	.	NNP
cord-345088-krb1eidw	208	44	,	,	,
cord-345088-krb1eidw	208	45	1995	1995	CD
cord-345088-krb1eidw	208	46	)	)	-RRB-
cord-345088-krb1eidw	208	47	.	.	.
cord-345088-krb1eidw	209	1	One	one	CD
cord-345088-krb1eidw	209	2	of	of	IN
cord-345088-krb1eidw	209	3	the	the	DT
cord-345088-krb1eidw	209	4	essential	essential	JJ
cord-345088-krb1eidw	209	5	steps	step	NNS
cord-345088-krb1eidw	209	6	in	in	IN
cord-345088-krb1eidw	209	7	Nlinked	Nlinked	NNP
cord-345088-krb1eidw	209	8	glycosylation	glycosylation	NN
cord-345088-krb1eidw	209	9	is	be	VBZ
cord-345088-krb1eidw	209	10	transfer	transfer	NN
cord-345088-krb1eidw	209	11	of	of	IN
cord-345088-krb1eidw	209	12	a	a	DT
cord-345088-krb1eidw	209	13	preformed	preformed	JJ
cord-345088-krb1eidw	209	14	,	,	,
cord-345088-krb1eidw	209	15	14-coreunit	14-coreunit	CD
cord-345088-krb1eidw	209	16	-	-	HYPH
cord-345088-krb1eidw	209	17	oligosaccharide	oligosaccharide	NN
cord-345088-krb1eidw	209	18	to	to	IN
cord-345088-krb1eidw	209	19	a	a	DT
cord-345088-krb1eidw	209	20	specific	specific	JJ
cord-345088-krb1eidw	209	21	Asn	Asn	NNP
cord-345088-krb1eidw	209	22	residue	residue	NN
cord-345088-krb1eidw	209	23	in	in	IN
cord-345088-krb1eidw	209	24	the	the	DT
cord-345088-krb1eidw	209	25	sequence	sequence	NN
cord-345088-krb1eidw	210	1	Asn	Asn	NNP
cord-345088-krb1eidw	210	2	-	-	HYPH
cord-345088-krb1eidw	210	3	X	X	NNP
cord-345088-krb1eidw	210	4	-	-	JJ
cord-345088-krb1eidw	210	5	Ser	Ser	NNP
cord-345088-krb1eidw	210	6	/	/	SYM
cord-345088-krb1eidw	210	7	Thr	Thr	NNP
cord-345088-krb1eidw	210	8	(	(	-LRB-
cord-345088-krb1eidw	210	9	where	where	WRB
cord-345088-krb1eidw	210	10	X	x	NN
cord-345088-krb1eidw	210	11	is	be	VBZ
cord-345088-krb1eidw	210	12	any	any	DT
cord-345088-krb1eidw	210	13	residue	residue	NN
cord-345088-krb1eidw	210	14	except	except	IN
cord-345088-krb1eidw	210	15	Pro	Pro	NNP
cord-345088-krb1eidw	210	16	,	,	,
cord-345088-krb1eidw	210	17	Asp	Asp	NNP
cord-345088-krb1eidw	210	18	,	,	,
cord-345088-krb1eidw	210	19	or	or	CC
cord-345088-krb1eidw	210	20	Glu	Glu	NNP
cord-345088-krb1eidw	210	21	)	)	-RRB-
cord-345088-krb1eidw	210	22	.	.	.
cord-345088-krb1eidw	211	1	The	the	DT
cord-345088-krb1eidw	211	2	oligosaccharide	oligosaccharide	NN
cord-345088-krb1eidw	211	3	chain	chain	NN
cord-345088-krb1eidw	211	4	is	be	VBZ
cord-345088-krb1eidw	211	5	donated	donate	VBN
cord-345088-krb1eidw	211	6	by	by	IN
cord-345088-krb1eidw	211	7	dolichol	dolichol	JJ
cord-345088-krb1eidw	211	8	-	-	HYPH
cord-345088-krb1eidw	211	9	pyrophosphate	pyrophosphate	NN
cord-345088-krb1eidw	211	10	-	-	HYPH
cord-345088-krb1eidw	211	11	oligosaccharide	oligosaccharide	NN
cord-345088-krb1eidw	211	12	to	to	IN
cord-345088-krb1eidw	211	13	the	the	DT
cord-345088-krb1eidw	211	14	protein	protein	NN
cord-345088-krb1eidw	211	15	chain	chain	NN
cord-345088-krb1eidw	211	16	in	in	IN
cord-345088-krb1eidw	211	17	the	the	DT
cord-345088-krb1eidw	211	18	ER	ER	NNP
cord-345088-krb1eidw	211	19	.	.	.
cord-345088-krb1eidw	212	1	It	-PRON-	PRP
cord-345088-krb1eidw	212	2	is	be	VBZ
cord-345088-krb1eidw	212	3	trimmed	trim	VBN
cord-345088-krb1eidw	212	4	down	down	RP
cord-345088-krb1eidw	212	5	in	in	IN
cord-345088-krb1eidw	212	6	the	the	DT
cord-345088-krb1eidw	212	7	ER	ER	NNP
cord-345088-krb1eidw	212	8	and	and	CC
cord-345088-krb1eidw	212	9	cis	cis	NNP
cord-345088-krb1eidw	212	10	-	-	HYPH
cord-345088-krb1eidw	212	11	Golgi	Golgi	NNP
cord-345088-krb1eidw	212	12	,	,	,
cord-345088-krb1eidw	212	13	and	and	CC
cord-345088-krb1eidw	212	14	different	different	JJ
cord-345088-krb1eidw	212	15	external	external	JJ
cord-345088-krb1eidw	212	16	sugars	sugar	NNS
cord-345088-krb1eidw	212	17	are	be	VBP
cord-345088-krb1eidw	212	18	then	then	RB
cord-345088-krb1eidw	212	19	added	add	VBN
cord-345088-krb1eidw	212	20	to	to	IN
cord-345088-krb1eidw	212	21	the	the	DT
cord-345088-krb1eidw	212	22	trimmed	trim	VBN
cord-345088-krb1eidw	212	23	chain	chain	NN
cord-345088-krb1eidw	212	24	in	in	IN
cord-345088-krb1eidw	212	25	the	the	DT
cord-345088-krb1eidw	212	26	medial	medial	JJ
cord-345088-krb1eidw	212	27	-	-	HYPH
cord-345088-krb1eidw	212	28	and	and	CC
cord-345088-krb1eidw	212	29	trans	trans	NNP
cord-345088-krb1eidw	212	30	-	-	NNP
cord-345088-krb1eidw	212	31	Golgi	Golgi	NNP
cord-345088-krb1eidw	212	32	.	.	.
cord-345088-krb1eidw	213	1	Glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	213	2	with	with	IN
cord-345088-krb1eidw	213	3	high	high	JJ
cord-345088-krb1eidw	213	4	mannose	mannose	NN
cord-345088-krb1eidw	213	5	oligosaccharides	oligosaccharide	NNS
cord-345088-krb1eidw	213	6	in	in	IN
cord-345088-krb1eidw	213	7	the	the	DT
cord-345088-krb1eidw	213	8	ER	ER	NNP
cord-345088-krb1eidw	213	9	and	and	CC
cord-345088-krb1eidw	213	10	cis	cis	NNP
cord-345088-krb1eidw	213	11	-	-	HYPH
cord-345088-krb1eidw	213	12	Golgi	Golgi	NNP
cord-345088-krb1eidw	213	13	remain	remain	VBP
cord-345088-krb1eidw	213	14	sensitive	sensitive	JJ
cord-345088-krb1eidw	213	15	to	to	IN
cord-345088-krb1eidw	213	16	Endo	Endo	NNP
cord-345088-krb1eidw	213	17	-	-	HYPH
cord-345088-krb1eidw	213	18	H.	H.	NNP
cord-345088-krb1eidw	214	1	They	-PRON-	PRP
cord-345088-krb1eidw	214	2	become	become	VBP
cord-345088-krb1eidw	214	3	Endo	Endo	NNP
cord-345088-krb1eidw	214	4	-	-	HYPH
cord-345088-krb1eidw	214	5	H	h	NN
cord-345088-krb1eidw	214	6	resistant	resistant	NN
cord-345088-krb1eidw	214	7	after	after	IN
cord-345088-krb1eidw	214	8	being	be	VBG
cord-345088-krb1eidw	214	9	processed	process	VBN
cord-345088-krb1eidw	214	10	by	by	IN
cord-345088-krb1eidw	214	11	the	the	DT
cord-345088-krb1eidw	214	12	medial	medial	JJ
cord-345088-krb1eidw	214	13	-	-	HYPH
cord-345088-krb1eidw	214	14	and	and	CC
cord-345088-krb1eidw	214	15	trans	trans	JJ
cord-345088-krb1eidw	214	16	-	-	JJ
cord-345088-krb1eidw	214	17	Golgi	Golgi	NNP
cord-345088-krb1eidw	214	18	resident	resident	NN
cord-345088-krb1eidw	214	19	enzymes	enzyme	NNS
cord-345088-krb1eidw	214	20	to	to	IN
cord-345088-krb1eidw	214	21	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	214	22	with	with	IN
cord-345088-krb1eidw	214	23	complex	complex	JJ
cord-345088-krb1eidw	214	24	oligosaccharides	oligosaccharide	NNS
cord-345088-krb1eidw	214	25	.	.	.
cord-345088-krb1eidw	215	1	The	the	DT
cord-345088-krb1eidw	215	2	acquisition	acquisition	NN
cord-345088-krb1eidw	215	3	of	of	IN
cord-345088-krb1eidw	215	4	Endo	Endo	NNP
cord-345088-krb1eidw	215	5	-	-	HYPH
cord-345088-krb1eidw	215	6	H	H	NNP
cord-345088-krb1eidw	215	7	resistance	resistance	NN
cord-345088-krb1eidw	215	8	is	be	VBZ
cord-345088-krb1eidw	215	9	therefore	therefore	RB
cord-345088-krb1eidw	215	10	an	an	DT
cord-345088-krb1eidw	215	11	indication	indication	NN
cord-345088-krb1eidw	215	12	that	that	IN
cord-345088-krb1eidw	215	13	viral	viral	JJ
cord-345088-krb1eidw	215	14	N	n	NN
cord-345088-krb1eidw	215	15	-	-	HYPH
cord-345088-krb1eidw	215	16	linked	link	VBN
cord-345088-krb1eidw	215	17	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	215	18	are	be	VBP
cord-345088-krb1eidw	215	19	properly	properly	RB
cord-345088-krb1eidw	215	20	processed	process	VBN
cord-345088-krb1eidw	215	21	and	and	CC
cord-345088-krb1eidw	215	22	transported	transport	VBN
cord-345088-krb1eidw	215	23	to	to	IN
cord-345088-krb1eidw	215	24	the	the	DT
cord-345088-krb1eidw	215	25	Golgi	Golgi	NNP
cord-345088-krb1eidw	215	26	apparatus	apparatus	NN
cord-345088-krb1eidw	215	27	(	(	-LRB-
cord-345088-krb1eidw	215	28	Luo	Luo	NNP
cord-345088-krb1eidw	215	29	and	and	CC
cord-345088-krb1eidw	215	30	Weiss	Weiss	NNP
cord-345088-krb1eidw	215	31	,	,	,
cord-345088-krb1eidw	215	32	1998	1998	CD
cord-345088-krb1eidw	215	33	;	;	:
cord-345088-krb1eidw	215	34	Luo	Luo	NNP
cord-345088-krb1eidw	215	35	et	et	NNP
cord-345088-krb1eidw	215	36	al	al	NNP
cord-345088-krb1eidw	215	37	.	.	NNP
cord-345088-krb1eidw	215	38	,	,	,
cord-345088-krb1eidw	215	39	1999	1999	CD
cord-345088-krb1eidw	215	40	;	;	:
cord-345088-krb1eidw	216	1	Luytjes	Luytjes	NNP
cord-345088-krb1eidw	216	2	et	et	NNP
cord-345088-krb1eidw	216	3	al	al	NNP
cord-345088-krb1eidw	216	4	.	.	NNP
cord-345088-krb1eidw	216	5	,	,	,
cord-345088-krb1eidw	216	6	1997	1997	CD
cord-345088-krb1eidw	216	7	)	)	-RRB-
cord-345088-krb1eidw	216	8	.	.	.
cord-345088-krb1eidw	217	1	Examination	examination	NN
cord-345088-krb1eidw	217	2	of	of	IN
cord-345088-krb1eidw	217	3	the	the	DT
cord-345088-krb1eidw	217	4	amino	amino	NN
cord-345088-krb1eidw	217	5	acid	acid	NN
cord-345088-krb1eidw	217	6	sequence	sequence	NN
cord-345088-krb1eidw	217	7	of	of	IN
cord-345088-krb1eidw	217	8	IBV	IBV	NNP
cord-345088-krb1eidw	217	9	S	S	NNP
cord-345088-krb1eidw	217	10	protein	protein	NN
cord-345088-krb1eidw	217	11	showed	show	VBD
cord-345088-krb1eidw	217	12	that	that	IN
cord-345088-krb1eidw	217	13	there	there	EX
cord-345088-krb1eidw	217	14	are	be	VBP
cord-345088-krb1eidw	217	15	13	13	CD
cord-345088-krb1eidw	217	16	and	and	CC
cord-345088-krb1eidw	217	17	9	9	CD
cord-345088-krb1eidw	217	18	potential	potential	JJ
cord-345088-krb1eidw	217	19	glycosylation	glycosylation	NN
cord-345088-krb1eidw	217	20	sites	site	NNS
cord-345088-krb1eidw	217	21	in	in	IN
cord-345088-krb1eidw	217	22	the	the	DT
cord-345088-krb1eidw	217	23	S1	S1	NNP
cord-345088-krb1eidw	217	24	and	and	CC
cord-345088-krb1eidw	217	25	S2	s2	NN
cord-345088-krb1eidw	217	26	subunits	subunit	NNS
cord-345088-krb1eidw	217	27	,	,	,
cord-345088-krb1eidw	217	28	respectively	respectively	RB
cord-345088-krb1eidw	217	29	.	.	.
cord-345088-krb1eidw	218	1	The	the	DT
cord-345088-krb1eidw	218	2	calculated	calculated	JJ
cord-345088-krb1eidw	218	3	molecular	molecular	JJ
cord-345088-krb1eidw	218	4	weight	weight	NN
cord-345088-krb1eidw	218	5	of	of	IN
cord-345088-krb1eidw	218	6	the	the	DT
cord-345088-krb1eidw	218	7	S	S	NNP
cord-345088-krb1eidw	218	8	protein	protein	NN
cord-345088-krb1eidw	218	9	is	be	VBZ
cord-345088-krb1eidw	218	10	128	128	CD
cord-345088-krb1eidw	218	11	kDa	kDa	NNS
cord-345088-krb1eidw	218	12	.	.	.
cord-345088-krb1eidw	219	1	If	if	IN
cord-345088-krb1eidw	219	2	all	all	DT
cord-345088-krb1eidw	219	3	21	21	CD
cord-345088-krb1eidw	219	4	sites	site	NNS
cord-345088-krb1eidw	219	5	are	be	VBP
cord-345088-krb1eidw	219	6	used	use	VBN
cord-345088-krb1eidw	219	7	,	,	,
cord-345088-krb1eidw	219	8	the	the	DT
cord-345088-krb1eidw	219	9	initially	initially	RB
cord-345088-krb1eidw	219	10	transferred	transfer	VBN
cord-345088-krb1eidw	219	11	oligosaccharides	oligosaccharide	NNS
cord-345088-krb1eidw	219	12	account	account	VBP
cord-345088-krb1eidw	219	13	for	for	IN
cord-345088-krb1eidw	219	14	50	50	CD
cord-345088-krb1eidw	219	15	kDa	kDa	NNS
cord-345088-krb1eidw	219	16	.	.	.
cord-345088-krb1eidw	220	1	In	in	IN
cord-345088-krb1eidw	220	2	cells	cell	NNS
cord-345088-krb1eidw	220	3	overexpressing	overexpresse	VBG
cord-345088-krb1eidw	220	4	S	S	NNP
cord-345088-krb1eidw	220	5	protein	protein	NN
cord-345088-krb1eidw	220	6	,	,	,
cord-345088-krb1eidw	220	7	core	core	NN
cord-345088-krb1eidw	220	8	-	-	HYPH
cord-345088-krb1eidw	220	9	glycosylation	glycosylation	NN
cord-345088-krb1eidw	220	10	of	of	IN
cord-345088-krb1eidw	220	11	the	the	DT
cord-345088-krb1eidw	220	12	S	S	NNP
cord-345088-krb1eidw	220	13	protein	protein	NN
cord-345088-krb1eidw	220	14	in	in	IN
cord-345088-krb1eidw	220	15	the	the	DT
cord-345088-krb1eidw	220	16	ER	ER	NNP
cord-345088-krb1eidw	220	17	resulted	result	VBD
cord-345088-krb1eidw	220	18	in	in	IN
cord-345088-krb1eidw	220	19	a	a	DT
cord-345088-krb1eidw	220	20	180-kDa	180-kda	CD
cord-345088-krb1eidw	220	21	product	product	NN
cord-345088-krb1eidw	220	22	as	as	IN
cord-345088-krb1eidw	220	23	expected	expect	VBN
cord-345088-krb1eidw	220	24	.	.	.
cord-345088-krb1eidw	221	1	Trimming	trim	VBG
cord-345088-krb1eidw	221	2	of	of	IN
cord-345088-krb1eidw	221	3	the	the	DT
cord-345088-krb1eidw	221	4	S	S	NNP
cord-345088-krb1eidw	221	5	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	221	6	in	in	IN
cord-345088-krb1eidw	221	7	the	the	DT
cord-345088-krb1eidw	221	8	ER	ER	NNP
cord-345088-krb1eidw	221	9	and	and	CC
cord-345088-krb1eidw	221	10	cis	cis	NNP
cord-345088-krb1eidw	221	11	-	-	HYPH
cord-345088-krb1eidw	221	12	Golgi	Golgi	NNP
cord-345088-krb1eidw	221	13	led	lead	VBD
cord-345088-krb1eidw	221	14	to	to	IN
cord-345088-krb1eidw	221	15	a	a	DT
cord-345088-krb1eidw	221	16	partially	partially	RB
cord-345088-krb1eidw	221	17	processed	process	VBN
cord-345088-krb1eidw	221	18	155-kDa	155-kda	CD
cord-345088-krb1eidw	221	19	form	form	NN
cord-345088-krb1eidw	221	20	,	,	,
cord-345088-krb1eidw	221	21	larger	large	JJR
cord-345088-krb1eidw	221	22	than	than	IN
cord-345088-krb1eidw	221	23	the	the	DT
cord-345088-krb1eidw	221	24	Endo	Endo	NNP
cord-345088-krb1eidw	221	25	-	-	HYPH
cord-345088-krb1eidw	221	26	H	H	NNP
cord-345088-krb1eidw	221	27	-	-	HYPH
cord-345088-krb1eidw	221	28	treated	treat	VBN
cord-345088-krb1eidw	221	29	130-kDa	130-kda	CD
cord-345088-krb1eidw	221	30	form	form	NN
cord-345088-krb1eidw	221	31	which	which	WDT
cord-345088-krb1eidw	221	32	only	only	RB
cord-345088-krb1eidw	221	33	has	have	VBZ
cord-345088-krb1eidw	221	34	one	one	CD
cord-345088-krb1eidw	221	35	sugar	sugar	NN
cord-345088-krb1eidw	221	36	residue	residue	NN
cord-345088-krb1eidw	221	37	left	leave	VBN
cord-345088-krb1eidw	221	38	at	at	IN
cord-345088-krb1eidw	221	39	each	each	DT
cord-345088-krb1eidw	221	40	site	site	NN
cord-345088-krb1eidw	221	41	.	.	.
cord-345088-krb1eidw	222	1	Both	both	DT
cord-345088-krb1eidw	222	2	the	the	DT
cord-345088-krb1eidw	222	3	trimmed	trimmed	JJ
cord-345088-krb1eidw	222	4	155-and	155-and	CD
cord-345088-krb1eidw	222	5	core	core	NN
cord-345088-krb1eidw	222	6	-	-	HYPH
cord-345088-krb1eidw	222	7	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	222	8	180-kDa	180-kda	CD
cord-345088-krb1eidw	222	9	forms	form	NNS
cord-345088-krb1eidw	222	10	were	be	VBD
cord-345088-krb1eidw	222	11	sensitive	sensitive	JJ
cord-345088-krb1eidw	222	12	to	to	IN
cord-345088-krb1eidw	222	13	Endo	endo	NN
cord-345088-krb1eidw	222	14	-	-	HYPH
cord-345088-krb1eidw	222	15	H	H	NNP
cord-345088-krb1eidw	222	16	digestion	digestion	NN
cord-345088-krb1eidw	222	17	.	.	.
cord-345088-krb1eidw	223	1	At	at	IN
cord-345088-krb1eidw	223	2	the	the	DT
cord-345088-krb1eidw	223	3	permissive	permissive	JJ
cord-345088-krb1eidw	223	4	temperature	temperature	NN
cord-345088-krb1eidw	223	5	,	,	,
cord-345088-krb1eidw	223	6	further	further	JJ
cord-345088-krb1eidw	223	7	modification	modification	NN
cord-345088-krb1eidw	223	8	of	of	IN
cord-345088-krb1eidw	223	9	the	the	DT
cord-345088-krb1eidw	223	10	mutant	mutant	JJ
cord-345088-krb1eidw	223	11	S	S	NNP
cord-345088-krb1eidw	223	12	protein	protein	NN
cord-345088-krb1eidw	223	13	in	in	IN
cord-345088-krb1eidw	223	14	the	the	DT
cord-345088-krb1eidw	223	15	medial	medial	JJ
cord-345088-krb1eidw	223	16	-	-	HYPH
cord-345088-krb1eidw	223	17	and	and	CC
cord-345088-krb1eidw	223	18	trans	trans	JJ
cord-345088-krb1eidw	223	19	-	-	JJ
cord-345088-krb1eidw	223	20	Golgi	Golgi	NNP
cord-345088-krb1eidw	223	21	by	by	IN
cord-345088-krb1eidw	223	22	addition	addition	NN
cord-345088-krb1eidw	223	23	of	of	IN
cord-345088-krb1eidw	223	24	sugars	sugar	NNS
cord-345088-krb1eidw	223	25	to	to	IN
cord-345088-krb1eidw	223	26	the	the	DT
cord-345088-krb1eidw	223	27	trimmed	trim	VBN
cord-345088-krb1eidw	223	28	chains	chain	NNS
cord-345088-krb1eidw	223	29	resulted	result	VBD
cord-345088-krb1eidw	223	30	in	in	IN
cord-345088-krb1eidw	223	31	the	the	DT
cord-345088-krb1eidw	223	32	maturation	maturation	NN
cord-345088-krb1eidw	223	33	of	of	IN
cord-345088-krb1eidw	223	34	S	S	NNP
cord-345088-krb1eidw	223	35	,	,	,
cord-345088-krb1eidw	223	36	which	which	WDT
cord-345088-krb1eidw	223	37	co	co	VBD
cord-345088-krb1eidw	223	38	-	-	VBZ
cord-345088-krb1eidw	223	39	migrates	migrate	VBZ
cord-345088-krb1eidw	223	40	with	with	IN
cord-345088-krb1eidw	223	41	the	the	DT
cord-345088-krb1eidw	223	42	core	core	NN
cord-345088-krb1eidw	223	43	-	-	HYPH
cord-345088-krb1eidw	223	44	glycosylated	glycosylated	JJ
cord-345088-krb1eidw	223	45	180-kDa	180-kda	CD
cord-345088-krb1eidw	223	46	form	form	NN
cord-345088-krb1eidw	223	47	but	but	CC
cord-345088-krb1eidw	223	48	is	be	VBZ
cord-345088-krb1eidw	223	49	resistant	resistant	JJ
cord-345088-krb1eidw	223	50	to	to	IN
cord-345088-krb1eidw	223	51	Endo	endo	NN
cord-345088-krb1eidw	223	52	-	-	HYPH
cord-345088-krb1eidw	223	53	H	H	NNP
cord-345088-krb1eidw	223	54	digestion	digestion	NN
cord-345088-krb1eidw	223	55	.	.	.
cord-345088-krb1eidw	224	1	At	at	IN
cord-345088-krb1eidw	224	2	the	the	DT
cord-345088-krb1eidw	224	3	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	224	4	temperature	temperature	NN
cord-345088-krb1eidw	224	5	,	,	,
cord-345088-krb1eidw	224	6	however	however	RB
cord-345088-krb1eidw	224	7	,	,	,
cord-345088-krb1eidw	224	8	no	no	DT
cord-345088-krb1eidw	224	9	mature	mature	JJ
cord-345088-krb1eidw	224	10	form	form	NN
cord-345088-krb1eidw	224	11	of	of	IN
cord-345088-krb1eidw	224	12	the	the	DT
cord-345088-krb1eidw	224	13	Fig	Fig	NNP
cord-345088-krb1eidw	224	14	.	.	.
cord-345088-krb1eidw	225	1	6	6	LS
cord-345088-krb1eidw	225	2	.	.	.
cord-345088-krb1eidw	226	1	Analysis	analysis	NN
cord-345088-krb1eidw	226	2	of	of	IN
cord-345088-krb1eidw	226	3	the	the	DT
cord-345088-krb1eidw	226	4	structural	structural	JJ
cord-345088-krb1eidw	226	5	proteins	protein	NNS
cord-345088-krb1eidw	226	6	on	on	IN
cord-345088-krb1eidw	226	7	purified	purify	VBN
cord-345088-krb1eidw	226	8	ts291602	ts291602	NNP
cord-345088-krb1eidw	226	9	and	and	CC
cord-345088-krb1eidw	226	10	rev-1	rev-1	NNP
cord-345088-krb1eidw	226	11	virions	virion	NNS
cord-345088-krb1eidw	226	12	.	.	.
cord-345088-krb1eidw	227	1	Immunoprecipitations	immunoprecipitation	NNS
cord-345088-krb1eidw	227	2	were	be	VBD
cord-345088-krb1eidw	227	3	performed	perform	VBN
cord-345088-krb1eidw	227	4	using	use	VBG
cord-345088-krb1eidw	227	5	virions	virion	NNS
cord-345088-krb1eidw	227	6	,	,	,
cord-345088-krb1eidw	227	7	purified	purify	VBN
cord-345088-krb1eidw	227	8	from	from	IN
cord-345088-krb1eidw	227	9	ts291602-(lanes	ts291602-(lanes	NNP
cord-345088-krb1eidw	227	10	1	1	CD
cord-345088-krb1eidw	227	11	and	and	CC
cord-345088-krb1eidw	227	12	2	2	CD
cord-345088-krb1eidw	227	13	)	)	-RRB-
cord-345088-krb1eidw	227	14	and	and	CC
cord-345088-krb1eidw	227	15	rev-1-infected	rev-1-infected	JJ
cord-345088-krb1eidw	228	1	(	(	-LRB-
cord-345088-krb1eidw	228	2	lanes	lane	NNS
cord-345088-krb1eidw	228	3	3	3	CD
cord-345088-krb1eidw	228	4	and	and	CC
cord-345088-krb1eidw	228	5	4	4	CD
cord-345088-krb1eidw	228	6	)	)	-RRB-
cord-345088-krb1eidw	228	7	Vero	Vero	NNP
cord-345088-krb1eidw	228	8	cells	cell	NNS
cord-345088-krb1eidw	228	9	at	at	IN
cord-345088-krb1eidw	228	10	32	32	CD
cord-345088-krb1eidw	228	11	jC	jC	NNS
cord-345088-krb1eidw	229	1	(	(	-LRB-
cord-345088-krb1eidw	229	2	lanes	lane	NNS
cord-345088-krb1eidw	229	3	1	1	CD
cord-345088-krb1eidw	229	4	and	and	CC
cord-345088-krb1eidw	229	5	3	3	CD
cord-345088-krb1eidw	229	6	)	)	-RRB-
cord-345088-krb1eidw	229	7	and	and	CC
cord-345088-krb1eidw	229	8	40	40	CD
cord-345088-krb1eidw	229	9	jC	jC	NNP
cord-345088-krb1eidw	229	10	(	(	-LRB-
cord-345088-krb1eidw	229	11	lanes	lane	NNS
cord-345088-krb1eidw	229	12	2	2	CD
cord-345088-krb1eidw	229	13	and	and	CC
cord-345088-krb1eidw	229	14	4	4	CD
cord-345088-krb1eidw	229	15	)	)	-RRB-
cord-345088-krb1eidw	229	16	.	.	.
cord-345088-krb1eidw	230	1	The	the	DT
cord-345088-krb1eidw	230	2	viral	viral	JJ
cord-345088-krb1eidw	230	3	proteins	protein	NNS
cord-345088-krb1eidw	230	4	were	be	VBD
cord-345088-krb1eidw	230	5	separated	separate	VBN
cord-345088-krb1eidw	230	6	on	on	IN
cord-345088-krb1eidw	230	7	12.5	12.5	CD
cord-345088-krb1eidw	230	8	%	%	NN
cord-345088-krb1eidw	230	9	polyacrylamide	polyacrylamide	NN
cord-345088-krb1eidw	230	10	gels	gel	NNS
cord-345088-krb1eidw	230	11	and	and	CC
cord-345088-krb1eidw	230	12	detected	detect	VBN
cord-345088-krb1eidw	230	13	by	by	IN
cord-345088-krb1eidw	230	14	autoradiography	autoradiography	NNP
cord-345088-krb1eidw	230	15	.	.	.
cord-345088-krb1eidw	231	1	Numbers	number	NNS
cord-345088-krb1eidw	231	2	on	on	IN
cord-345088-krb1eidw	231	3	the	the	DT
cord-345088-krb1eidw	231	4	left	left	JJ
cord-345088-krb1eidw	231	5	indicate	indicate	VBP
cord-345088-krb1eidw	231	6	molecular	molecular	JJ
cord-345088-krb1eidw	231	7	masses	masse	NNS
cord-345088-krb1eidw	231	8	in	in	IN
cord-345088-krb1eidw	231	9	kilodalton	kilodalton	NNP
cord-345088-krb1eidw	231	10	and	and	CC
cord-345088-krb1eidw	231	11	the	the	DT
cord-345088-krb1eidw	231	12	positions	position	NNS
cord-345088-krb1eidw	231	13	of	of	IN
cord-345088-krb1eidw	231	14	the	the	DT
cord-345088-krb1eidw	231	15	three	three	CD
cord-345088-krb1eidw	231	16	structural	structural	JJ
cord-345088-krb1eidw	231	17	proteins	protein	NNS
cord-345088-krb1eidw	231	18	are	be	VBP
cord-345088-krb1eidw	231	19	indicated	indicate	VBN
cord-345088-krb1eidw	231	20	on	on	IN
cord-345088-krb1eidw	231	21	the	the	DT
cord-345088-krb1eidw	231	22	right	right	NN
cord-345088-krb1eidw	231	23	.	.	.
cord-345088-krb1eidw	232	1	mutant	mutant	NNP
cord-345088-krb1eidw	232	2	S	S	NNP
cord-345088-krb1eidw	232	3	protein	protein	NN
cord-345088-krb1eidw	232	4	was	be	VBD
cord-345088-krb1eidw	232	5	produced	produce	VBN
cord-345088-krb1eidw	232	6	.	.	.
cord-345088-krb1eidw	233	1	In	in	IN
cord-345088-krb1eidw	233	2	virus	virus	NN
cord-345088-krb1eidw	233	3	-	-	HYPH
cord-345088-krb1eidw	233	4	infected	infect	VBN
cord-345088-krb1eidw	233	5	cells	cell	NNS
cord-345088-krb1eidw	233	6	,	,	,
cord-345088-krb1eidw	233	7	the	the	DT
cord-345088-krb1eidw	233	8	Endo	Endo	NNP
cord-345088-krb1eidw	233	9	-	-	HYPH
cord-345088-krb1eidw	233	10	H	H	NNP
cord-345088-krb1eidw	233	11	sensitive	sensitive	JJ
cord-345088-krb1eidw	233	12	,	,	,
cord-345088-krb1eidw	233	13	155-kDa	155-kda	CD
cord-345088-krb1eidw	233	14	form	form	NN
cord-345088-krb1eidw	233	15	of	of	IN
cord-345088-krb1eidw	233	16	ts291602	ts291602	NNP
cord-345088-krb1eidw	233	17	was	be	VBD
cord-345088-krb1eidw	233	18	abundant	abundant	JJ
cord-345088-krb1eidw	233	19	at	at	IN
cord-345088-krb1eidw	233	20	the	the	DT
cord-345088-krb1eidw	233	21	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	233	22	temperature	temperature	NN
cord-345088-krb1eidw	233	23	,	,	,
cord-345088-krb1eidw	233	24	clearly	clearly	RB
cord-345088-krb1eidw	233	25	indicating	indicate	VBG
cord-345088-krb1eidw	233	26	that	that	IN
cord-345088-krb1eidw	233	27	the	the	DT
cord-345088-krb1eidw	233	28	mutant	mutant	JJ
cord-345088-krb1eidw	233	29	S	S	NNP
cord-345088-krb1eidw	233	30	protein	protein	NN
cord-345088-krb1eidw	233	31	was	be	VBD
cord-345088-krb1eidw	233	32	trimmed	trim	VBN
cord-345088-krb1eidw	233	33	in	in	IN
cord-345088-krb1eidw	233	34	the	the	DT
cord-345088-krb1eidw	233	35	ER	ER	NNP
cord-345088-krb1eidw	233	36	and	and	CC
cord-345088-krb1eidw	233	37	cis	cis	NNP
cord-345088-krb1eidw	233	38	-	-	HYPH
cord-345088-krb1eidw	233	39	Golgi	Golgi	NNP
cord-345088-krb1eidw	233	40	but	but	CC
cord-345088-krb1eidw	233	41	was	be	VBD
cord-345088-krb1eidw	233	42	not	not	RB
cord-345088-krb1eidw	233	43	transported	transport	VBN
cord-345088-krb1eidw	233	44	to	to	IN
cord-345088-krb1eidw	233	45	the	the	DT
cord-345088-krb1eidw	233	46	trans	trans	NNP
cord-345088-krb1eidw	233	47	-	-	NNP
cord-345088-krb1eidw	233	48	Golgi	Golgi	NNP
cord-345088-krb1eidw	233	49	.	.	.
cord-345088-krb1eidw	234	1	Furthermore	furthermore	RB
cord-345088-krb1eidw	234	2	,	,	,
cord-345088-krb1eidw	234	3	the	the	DT
cord-345088-krb1eidw	234	4	mutant	mutant	JJ
cord-345088-krb1eidw	234	5	S	S	NNP
cord-345088-krb1eidw	234	6	protein	protein	NN
cord-345088-krb1eidw	234	7	was	be	VBD
cord-345088-krb1eidw	234	8	not	not	RB
cord-345088-krb1eidw	234	9	cleaved	cleave	VBN
cord-345088-krb1eidw	234	10	into	into	IN
cord-345088-krb1eidw	234	11	S1	S1	NNP
cord-345088-krb1eidw	234	12	and	and	CC
cord-345088-krb1eidw	234	13	S2	S2	NNP
cord-345088-krb1eidw	234	14	at	at	IN
cord-345088-krb1eidw	234	15	the	the	DT
cord-345088-krb1eidw	234	16	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	234	17	temperature	temperature	NN
cord-345088-krb1eidw	234	18	,	,	,
cord-345088-krb1eidw	234	19	which	which	WDT
cord-345088-krb1eidw	234	20	usually	usually	RB
cord-345088-krb1eidw	234	21	occurs	occur	VBZ
cord-345088-krb1eidw	234	22	in	in	IN
cord-345088-krb1eidw	234	23	the	the	DT
cord-345088-krb1eidw	234	24	trans	trans	NNP
cord-345088-krb1eidw	234	25	-	-	NNP
cord-345088-krb1eidw	234	26	Golgi	Golgi	NNP
cord-345088-krb1eidw	234	27	shortly	shortly	RB
cord-345088-krb1eidw	234	28	before	before	IN
cord-345088-krb1eidw	234	29	the	the	DT
cord-345088-krb1eidw	234	30	release	release	NN
cord-345088-krb1eidw	234	31	of	of	IN
cord-345088-krb1eidw	234	32	virions	virion	NNS
cord-345088-krb1eidw	234	33	from	from	IN
cord-345088-krb1eidw	234	34	cells	cell	NNS
cord-345088-krb1eidw	234	35	(	(	-LRB-
cord-345088-krb1eidw	234	36	Frana	Frana	NNP
cord-345088-krb1eidw	234	37	et	et	FW
cord-345088-krb1eidw	234	38	al	al	NNP
cord-345088-krb1eidw	234	39	.	.	NNP
cord-345088-krb1eidw	234	40	,	,	,
cord-345088-krb1eidw	234	41	1985	1985	CD
cord-345088-krb1eidw	234	42	)	)	-RRB-
cord-345088-krb1eidw	234	43	.	.	.
cord-345088-krb1eidw	235	1	As	as	IN
cord-345088-krb1eidw	235	2	only	only	JJ
cord-345088-krb1eidw	235	3	spikeless	spikeless	NN
cord-345088-krb1eidw	235	4	virions	virion	NNS
cord-345088-krb1eidw	235	5	were	be	VBD
cord-345088-krb1eidw	235	6	produced	produce	VBN
cord-345088-krb1eidw	235	7	,	,	,
cord-345088-krb1eidw	235	8	these	these	DT
cord-345088-krb1eidw	235	9	results	result	NNS
cord-345088-krb1eidw	235	10	demonstrated	demonstrate	VBD
cord-345088-krb1eidw	235	11	that	that	IN
cord-345088-krb1eidw	235	12	the	the	DT
cord-345088-krb1eidw	235	13	core	core	NN
cord-345088-krb1eidw	235	14	-	-	HYPH
cord-345088-krb1eidw	235	15	glycosylated	glycosylate	VBN
cord-345088-krb1eidw	235	16	or	or	CC
cord-345088-krb1eidw	235	17	the	the	DT
cord-345088-krb1eidw	235	18	trimmed	trim	VBN
cord-345088-krb1eidw	235	19	forms	form	NNS
cord-345088-krb1eidw	235	20	of	of	IN
cord-345088-krb1eidw	235	21	S	S	NNP
cord-345088-krb1eidw	235	22	were	be	VBD
cord-345088-krb1eidw	235	23	unable	unable	JJ
cord-345088-krb1eidw	235	24	to	to	TO
cord-345088-krb1eidw	235	25	incorporate	incorporate	VB
cord-345088-krb1eidw	235	26	into	into	IN
cord-345088-krb1eidw	235	27	virions	virion	NNS
cord-345088-krb1eidw	235	28	.	.	.
cord-345088-krb1eidw	236	1	In	in	IN
cord-345088-krb1eidw	236	2	addition	addition	NN
cord-345088-krb1eidw	236	3	,	,	,
cord-345088-krb1eidw	236	4	the	the	DT
cord-345088-krb1eidw	236	5	mutant	mutant	JJ
cord-345088-krb1eidw	236	6	S	S	NNP
cord-345088-krb1eidw	236	7	protein	protein	NN
cord-345088-krb1eidw	236	8	fails	fail	VBZ
cord-345088-krb1eidw	236	9	to	to	TO
cord-345088-krb1eidw	236	10	induce	induce	VB
cord-345088-krb1eidw	236	11	cell	cell	NN
cord-345088-krb1eidw	236	12	-cell	-cell	JJR
cord-345088-krb1eidw	236	13	fusion	fusion	NN
cord-345088-krb1eidw	236	14	at	at	IN
cord-345088-krb1eidw	236	15	the	the	DT
cord-345088-krb1eidw	236	16	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	236	17	temperature	temperature	NN
cord-345088-krb1eidw	236	18	.	.	.
cord-345088-krb1eidw	237	1	As	as	IN
cord-345088-krb1eidw	237	2	the	the	DT
cord-345088-krb1eidw	237	3	Q	Q	NNP
cord-345088-krb1eidw	237	4	294	294	CD
cord-345088-krb1eidw	237	5	-to	-to	NNPS
cord-345088-krb1eidw	237	6	-	-	:
cord-345088-krb1eidw	237	7	L	l	NN
cord-345088-krb1eidw	237	8	294	294	CD
cord-345088-krb1eidw	237	9	mutation	mutation	NN
cord-345088-krb1eidw	237	10	is	be	VBZ
cord-345088-krb1eidw	237	11	not	not	RB
cord-345088-krb1eidw	237	12	in	in	IN
cord-345088-krb1eidw	237	13	the	the	DT
cord-345088-krb1eidw	237	14	fusogenic	fusogenic	JJ
cord-345088-krb1eidw	237	15	domain	domain	NN
cord-345088-krb1eidw	237	16	of	of	IN
cord-345088-krb1eidw	237	17	S	S	NNP
cord-345088-krb1eidw	237	18	,	,	,
cord-345088-krb1eidw	237	19	it	-PRON-	PRP
cord-345088-krb1eidw	237	20	renders	render	VBZ
cord-345088-krb1eidw	237	21	support	support	NN
cord-345088-krb1eidw	237	22	to	to	IN
cord-345088-krb1eidw	237	23	the	the	DT
cord-345088-krb1eidw	237	24	conclusion	conclusion	NN
cord-345088-krb1eidw	237	25	that	that	IN
cord-345088-krb1eidw	237	26	the	the	DT
cord-345088-krb1eidw	237	27	mutation	mutation	NN
cord-345088-krb1eidw	237	28	causes	cause	VBZ
cord-345088-krb1eidw	237	29	the	the	DT
cord-345088-krb1eidw	237	30	defect	defect	NN
cord-345088-krb1eidw	237	31	in	in	IN
cord-345088-krb1eidw	237	32	its	-PRON-	PRP$
cord-345088-krb1eidw	237	33	modification	modification	NN
cord-345088-krb1eidw	237	34	and	and	CC
cord-345088-krb1eidw	237	35	intracellular	intracellular	JJ
cord-345088-krb1eidw	237	36	transport	transport	NN
cord-345088-krb1eidw	237	37	,	,	,
cord-345088-krb1eidw	237	38	instead	instead	RB
cord-345088-krb1eidw	237	39	of	of	IN
cord-345088-krb1eidw	237	40	having	have	VBG
cord-345088-krb1eidw	237	41	direct	direct	JJ
cord-345088-krb1eidw	237	42	impact	impact	NN
cord-345088-krb1eidw	237	43	on	on	IN
cord-345088-krb1eidw	237	44	the	the	DT
cord-345088-krb1eidw	237	45	fusion	fusion	NN
cord-345088-krb1eidw	237	46	process	process	NN
cord-345088-krb1eidw	237	47	.	.	.
cord-345088-krb1eidw	238	1	Taken	take	VBN
cord-345088-krb1eidw	238	2	together	together	RB
cord-345088-krb1eidw	238	3	,	,	,
cord-345088-krb1eidw	238	4	these	these	DT
cord-345088-krb1eidw	238	5	studies	study	NNS
cord-345088-krb1eidw	238	6	clearly	clearly	RB
cord-345088-krb1eidw	238	7	indicate	indicate	VBP
cord-345088-krb1eidw	238	8	that	that	IN
cord-345088-krb1eidw	238	9	the	the	DT
cord-345088-krb1eidw	238	10	mutant	mutant	JJ
cord-345088-krb1eidw	238	11	S	S	NNP
cord-345088-krb1eidw	238	12	protein	protein	NN
cord-345088-krb1eidw	238	13	could	could	MD
cord-345088-krb1eidw	238	14	not	not	RB
cord-345088-krb1eidw	238	15	reach	reach	VB
cord-345088-krb1eidw	238	16	the	the	DT
cord-345088-krb1eidw	238	17	site	site	NN
cord-345088-krb1eidw	238	18	where	where	WRB
cord-345088-krb1eidw	238	19	the	the	DT
cord-345088-krb1eidw	238	20	S	S	NNP
cord-345088-krb1eidw	238	21	protein	protein	NN
cord-345088-krb1eidw	238	22	is	be	VBZ
cord-345088-krb1eidw	238	23	incorporated	incorporate	VBN
cord-345088-krb1eidw	238	24	into	into	IN
cord-345088-krb1eidw	238	25	virions	virion	NNS
cord-345088-krb1eidw	238	26	.	.	.
cord-345088-krb1eidw	239	1	The	the	DT
cord-345088-krb1eidw	239	2	reasons	reason	NNS
cord-345088-krb1eidw	239	3	for	for	IN
cord-345088-krb1eidw	239	4	the	the	DT
cord-345088-krb1eidw	239	5	retention	retention	NN
cord-345088-krb1eidw	239	6	of	of	IN
cord-345088-krb1eidw	239	7	the	the	DT
cord-345088-krb1eidw	239	8	mutant	mutant	JJ
cord-345088-krb1eidw	239	9	S	S	NNP
cord-345088-krb1eidw	239	10	protein	protein	NN
cord-345088-krb1eidw	239	11	in	in	IN
cord-345088-krb1eidw	239	12	the	the	DT
cord-345088-krb1eidw	239	13	ER	ER	NNP
cord-345088-krb1eidw	239	14	and	and	CC
cord-345088-krb1eidw	239	15	cis	cis	NNP
cord-345088-krb1eidw	239	16	-	-	HYPH
cord-345088-krb1eidw	239	17	Golgi	Golgi	NNP
cord-345088-krb1eidw	239	18	are	be	VBP
cord-345088-krb1eidw	239	19	yet	yet	RB
cord-345088-krb1eidw	239	20	to	to	TO
cord-345088-krb1eidw	239	21	be	be	VB
cord-345088-krb1eidw	239	22	explored	explore	VBN
cord-345088-krb1eidw	239	23	.	.	.
cord-345088-krb1eidw	240	1	Previous	previous	JJ
cord-345088-krb1eidw	240	2	studies	study	NNS
cord-345088-krb1eidw	240	3	show	show	VBP
cord-345088-krb1eidw	240	4	that	that	IN
cord-345088-krb1eidw	240	5	the	the	DT
cord-345088-krb1eidw	240	6	retention	retention	NN
cord-345088-krb1eidw	240	7	of	of	IN
cord-345088-krb1eidw	240	8	monomeric	monomeric	JJ
cord-345088-krb1eidw	240	9	S	S	NNP
cord-345088-krb1eidw	240	10	protein	protein	NN
cord-345088-krb1eidw	240	11	in	in	IN
cord-345088-krb1eidw	240	12	the	the	DT
cord-345088-krb1eidw	240	13	ER	ER	NNP
cord-345088-krb1eidw	240	14	is	be	VBZ
cord-345088-krb1eidw	240	15	due	due	JJ
cord-345088-krb1eidw	240	16	to	to	IN
cord-345088-krb1eidw	240	17	misfolding	misfolde	VBG
cord-345088-krb1eidw	240	18	induced	induce	VBN
cord-345088-krb1eidw	240	19	by	by	IN
cord-345088-krb1eidw	240	20	disruption	disruption	NN
cord-345088-krb1eidw	240	21	of	of	IN
cord-345088-krb1eidw	240	22	disulfide	disulfide	JJ
cord-345088-krb1eidw	240	23	bonds	bond	NNS
cord-345088-krb1eidw	240	24	(	(	-LRB-
cord-345088-krb1eidw	241	1	Opstelten	Opstelten	NNP
cord-345088-krb1eidw	241	2	et	et	NNP
cord-345088-krb1eidw	241	3	al	al	NNP
cord-345088-krb1eidw	241	4	.	.	NNP
cord-345088-krb1eidw	241	5	,	,	,
cord-345088-krb1eidw	241	6	1993	1993	CD
cord-345088-krb1eidw	241	7	)	)	-RRB-
cord-345088-krb1eidw	241	8	,	,	,
cord-345088-krb1eidw	241	9	and	and	CC
cord-345088-krb1eidw	241	10	also	also	RB
cord-345088-krb1eidw	241	11	caused	cause	VBN
cord-345088-krb1eidw	241	12	by	by	IN
cord-345088-krb1eidw	241	13	lack	lack	NN
cord-345088-krb1eidw	241	14	of	of	IN
cord-345088-krb1eidw	241	15	oligomerization	oligomerization	NN
cord-345088-krb1eidw	241	16	of	of	IN
cord-345088-krb1eidw	241	17	Endo	Endo	NNP
cord-345088-krb1eidw	241	18	-	-	HYPH
cord-345088-krb1eidw	241	19	H	H	NNP
cord-345088-krb1eidw	241	20	-	-	HYPH
cord-345088-krb1eidw	241	21	sensitive	sensitive	JJ
cord-345088-krb1eidw	241	22	S	S	NNP
cord-345088-krb1eidw	241	23	protein	protein	NN
cord-345088-krb1eidw	241	24	of	of	IN
cord-345088-krb1eidw	241	25	ts	ts	JJ
cord-345088-krb1eidw	241	26	mutants	mutant	NNS
cord-345088-krb1eidw	241	27	though	though	IN
cord-345088-krb1eidw	241	28	the	the	DT
cord-345088-krb1eidw	241	29	locations	location	NNS
cord-345088-krb1eidw	241	30	were	be	VBD
cord-345088-krb1eidw	241	31	not	not	RB
cord-345088-krb1eidw	241	32	determined	determine	VBN
cord-345088-krb1eidw	242	1	(	(	-LRB-
cord-345088-krb1eidw	242	2	Luytjes	Luytjes	NNP
cord-345088-krb1eidw	242	3	et	et	NNP
cord-345088-krb1eidw	242	4	al	al	NNP
cord-345088-krb1eidw	242	5	.	.	NNP
cord-345088-krb1eidw	242	6	,	,	,
cord-345088-krb1eidw	242	7	1997	1997	CD
cord-345088-krb1eidw	242	8	)	)	-RRB-
cord-345088-krb1eidw	242	9	.	.	.
cord-345088-krb1eidw	243	1	It	-PRON-	PRP
cord-345088-krb1eidw	243	2	is	be	VBZ
cord-345088-krb1eidw	243	3	not	not	RB
cord-345088-krb1eidw	243	4	clear	clear	JJ
cord-345088-krb1eidw	243	5	whether	whether	IN
cord-345088-krb1eidw	243	6	mutations	mutation	NNS
cord-345088-krb1eidw	243	7	in	in	IN
cord-345088-krb1eidw	243	8	IBV	IBV	NNP
cord-345088-krb1eidw	243	9	S	S	NNP
cord-345088-krb1eidw	243	10	protein	protein	NN
cord-345088-krb1eidw	243	11	have	have	VBP
cord-345088-krb1eidw	243	12	an	an	DT
cord-345088-krb1eidw	243	13	effect	effect	NN
cord-345088-krb1eidw	243	14	on	on	IN
cord-345088-krb1eidw	243	15	its	-PRON-	PRP$
cord-345088-krb1eidw	243	16	folding	folding	NN
cord-345088-krb1eidw	243	17	or	or	CC
cord-345088-krb1eidw	243	18	oligomerization	oligomerization	NN
cord-345088-krb1eidw	243	19	.	.	.
cord-345088-krb1eidw	244	1	Nevertheless	nevertheless	RB
cord-345088-krb1eidw	244	2	,	,	,
cord-345088-krb1eidw	244	3	in	in	IN
cord-345088-krb1eidw	244	4	both	both	DT
cord-345088-krb1eidw	244	5	circumstances	circumstance	NNS
cord-345088-krb1eidw	244	6	,	,	,
cord-345088-krb1eidw	244	7	the	the	DT
cord-345088-krb1eidw	244	8	intracellular	intracellular	JJ
cord-345088-krb1eidw	244	9	transport	transport	NN
cord-345088-krb1eidw	244	10	,	,	,
cord-345088-krb1eidw	244	11	modification	modification	NN
cord-345088-krb1eidw	244	12	of	of	IN
cord-345088-krb1eidw	244	13	the	the	DT
cord-345088-krb1eidw	244	14	S	S	NNP
cord-345088-krb1eidw	244	15	protein	protein	NN
cord-345088-krb1eidw	244	16	and	and	CC
cord-345088-krb1eidw	244	17	its	-PRON-	PRP$
cord-345088-krb1eidw	244	18	incorporation	incorporation	NN
cord-345088-krb1eidw	244	19	into	into	IN
cord-345088-krb1eidw	244	20	virion	virion	NN
cord-345088-krb1eidw	244	21	would	would	MD
cord-345088-krb1eidw	244	22	have	have	VB
cord-345088-krb1eidw	244	23	been	be	VBN
cord-345088-krb1eidw	244	24	affected	affect	VBN
cord-345088-krb1eidw	244	25	.	.	.
cord-345088-krb1eidw	245	1	Studies	study	NNS
cord-345088-krb1eidw	245	2	with	with	IN
cord-345088-krb1eidw	245	3	some	some	DT
cord-345088-krb1eidw	245	4	of	of	IN
cord-345088-krb1eidw	245	5	the	the	DT
cord-345088-krb1eidw	245	6	other	other	JJ
cord-345088-krb1eidw	245	7	enveloped	envelop	VBN
cord-345088-krb1eidw	245	8	viruses	virus	NNS
cord-345088-krb1eidw	245	9	demonstrated	demonstrate	VBN
cord-345088-krb1eidw	245	10	that	that	IN
cord-345088-krb1eidw	245	11	molecular	molecular	JJ
cord-345088-krb1eidw	245	12	chaperones	chaperone	NNS
cord-345088-krb1eidw	245	13	(	(	-LRB-
cord-345088-krb1eidw	245	14	like	like	IN
cord-345088-krb1eidw	245	15	calnexin	calnexin	NNP
cord-345088-krb1eidw	245	16	and	and	CC
cord-345088-krb1eidw	245	17	calreticulin	calreticulin	NNP
cord-345088-krb1eidw	245	18	)	)	-RRB-
cord-345088-krb1eidw	245	19	in	in	IN
cord-345088-krb1eidw	245	20	the	the	DT
cord-345088-krb1eidw	245	21	ER	ER	NNP
cord-345088-krb1eidw	245	22	transiently	transiently	RB
cord-345088-krb1eidw	245	23	and	and	CC
cord-345088-krb1eidw	245	24	specifically	specifically	RB
cord-345088-krb1eidw	245	25	interact	interact	VB
cord-345088-krb1eidw	245	26	with	with	IN
cord-345088-krb1eidw	245	27	partially	partially	RB
cord-345088-krb1eidw	245	28	trimmed	trim	VBN
cord-345088-krb1eidw	245	29	,	,	,
cord-345088-krb1eidw	245	30	monoglycosylated	monoglycosylate	VBN
cord-345088-krb1eidw	245	31	form	form	NN
cord-345088-krb1eidw	245	32	of	of	IN
cord-345088-krb1eidw	245	33	viral	viral	JJ
cord-345088-krb1eidw	245	34	N	n	NN
cord-345088-krb1eidw	245	35	-	-	HYPH
cord-345088-krb1eidw	245	36	linked	link	VBN
cord-345088-krb1eidw	245	37	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	245	38	(	(	-LRB-
cord-345088-krb1eidw	245	39	Tatu	Tatu	NNP
cord-345088-krb1eidw	245	40	and	and	CC
cord-345088-krb1eidw	245	41	Helenius	Helenius	NNP
cord-345088-krb1eidw	245	42	,	,	,
cord-345088-krb1eidw	245	43	1997	1997	CD
cord-345088-krb1eidw	245	44	)	)	-RRB-
cord-345088-krb1eidw	245	45	.	.	.
cord-345088-krb1eidw	246	1	For	for	IN
cord-345088-krb1eidw	246	2	instance	instance	NN
cord-345088-krb1eidw	246	3	,	,	,
cord-345088-krb1eidw	246	4	calnexin	calnexin	NNP
cord-345088-krb1eidw	246	5	interacts	interact	VBZ
cord-345088-krb1eidw	246	6	with	with	IN
cord-345088-krb1eidw	246	7	the	the	DT
cord-345088-krb1eidw	246	8	influenza	influenza	NN
cord-345088-krb1eidw	246	9	hemagglutinin	hemagglutinin	NN
cord-345088-krb1eidw	246	10	and	and	CC
cord-345088-krb1eidw	246	11	vesicular	vesicular	JJ
cord-345088-krb1eidw	246	12	stomatitis	stomatitis	NN
cord-345088-krb1eidw	246	13	virus	virus	NN
cord-345088-krb1eidw	246	14	G	g	NN
cord-345088-krb1eidw	246	15	protein	protein	NN
cord-345088-krb1eidw	246	16	(	(	-LRB-
cord-345088-krb1eidw	246	17	Hebert	Hebert	NNP
cord-345088-krb1eidw	246	18	et	et	NNP
cord-345088-krb1eidw	246	19	al	al	NNP
cord-345088-krb1eidw	246	20	.	.	NNP
cord-345088-krb1eidw	246	21	,	,	,
cord-345088-krb1eidw	246	22	1996	1996	CD
cord-345088-krb1eidw	246	23	;	;	:
cord-345088-krb1eidw	246	24	Hammond	Hammond	NNP
cord-345088-krb1eidw	246	25	and	and	CC
cord-345088-krb1eidw	246	26	Helenius	Helenius	NNP
cord-345088-krb1eidw	246	27	,	,	,
cord-345088-krb1eidw	246	28	1994	1994	CD
cord-345088-krb1eidw	246	29	)	)	-RRB-
cord-345088-krb1eidw	246	30	.	.	.
cord-345088-krb1eidw	247	1	These	these	DT
cord-345088-krb1eidw	247	2	and	and	CC
cord-345088-krb1eidw	247	3	other	other	JJ
cord-345088-krb1eidw	247	4	proteins	protein	NNS
cord-345088-krb1eidw	247	5	could	could	MD
cord-345088-krb1eidw	247	6	facilitate	facilitate	VB
cord-345088-krb1eidw	247	7	proper	proper	JJ
cord-345088-krb1eidw	247	8	folding	folding	NN
cord-345088-krb1eidw	247	9	and	and	CC
cord-345088-krb1eidw	247	10	ensure	ensure	VB
cord-345088-krb1eidw	247	11	quality	quality	NN
cord-345088-krb1eidw	247	12	control	control	NN
cord-345088-krb1eidw	247	13	for	for	IN
cord-345088-krb1eidw	247	14	viral	viral	JJ
cord-345088-krb1eidw	247	15	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	247	16	.	.	.
cord-345088-krb1eidw	248	1	Proteins	protein	NNS
cord-345088-krb1eidw	248	2	that	that	WDT
cord-345088-krb1eidw	248	3	fail	fail	VBP
cord-345088-krb1eidw	248	4	to	to	TO
cord-345088-krb1eidw	248	5	fold	fold	VB
cord-345088-krb1eidw	248	6	and	and	CC
cord-345088-krb1eidw	248	7	oligomerize	oligomerize	NNP
cord-345088-krb1eidw	248	8	are	be	VBP
cord-345088-krb1eidw	248	9	prevented	prevent	VBN
cord-345088-krb1eidw	248	10	from	from	IN
cord-345088-krb1eidw	248	11	transport	transport	NN
cord-345088-krb1eidw	248	12	and	and	CC
cord-345088-krb1eidw	248	13	ultimately	ultimately	RB
cord-345088-krb1eidw	248	14	degraded	degrade	VBN
cord-345088-krb1eidw	248	15	.	.	.
cord-345088-krb1eidw	249	1	With	with	IN
cord-345088-krb1eidw	249	2	the	the	DT
cord-345088-krb1eidw	249	3	ts	ts	NNP
cord-345088-krb1eidw	249	4	mutants	mutant	NNS
cord-345088-krb1eidw	249	5	,	,	,
cord-345088-krb1eidw	249	6	it	-PRON-	PRP
cord-345088-krb1eidw	249	7	is	be	VBZ
cord-345088-krb1eidw	249	8	possible	possible	JJ
cord-345088-krb1eidw	249	9	to	to	TO
cord-345088-krb1eidw	249	10	explore	explore	VB
cord-345088-krb1eidw	249	11	the	the	DT
cord-345088-krb1eidw	249	12	interaction	interaction	NN
cord-345088-krb1eidw	249	13	of	of	IN
cord-345088-krb1eidw	249	14	the	the	DT
cord-345088-krb1eidw	249	15	S	S	NNP
cord-345088-krb1eidw	249	16	protein	protein	NN
cord-345088-krb1eidw	249	17	with	with	IN
cord-345088-krb1eidw	249	18	molecular	molecular	JJ
cord-345088-krb1eidw	249	19	chaperones	chaperone	NNS
cord-345088-krb1eidw	249	20	and	and	CC
cord-345088-krb1eidw	249	21	folding	folding	NN
cord-345088-krb1eidw	249	22	enzymes	enzyme	NNS
cord-345088-krb1eidw	249	23	.	.	.
cord-345088-krb1eidw	250	1	This	this	DT
cord-345088-krb1eidw	250	2	approach	approach	NN
cord-345088-krb1eidw	250	3	would	would	MD
cord-345088-krb1eidw	250	4	provide	provide	VB
cord-345088-krb1eidw	250	5	new	new	JJ
cord-345088-krb1eidw	250	6	insights	insight	NNS
cord-345088-krb1eidw	250	7	into	into	IN
cord-345088-krb1eidw	250	8	the	the	DT
cord-345088-krb1eidw	250	9	maturation	maturation	NN
cord-345088-krb1eidw	250	10	and	and	CC
cord-345088-krb1eidw	250	11	assembly	assembly	NN
cord-345088-krb1eidw	250	12	of	of	IN
cord-345088-krb1eidw	250	13	the	the	DT
cord-345088-krb1eidw	250	14	coronavirus	coronavirus	NN
cord-345088-krb1eidw	250	15	S	S	NNP
cord-345088-krb1eidw	250	16	protein	protein	NN
cord-345088-krb1eidw	250	17	.	.	.
cord-345088-krb1eidw	251	1	The	the	DT
cord-345088-krb1eidw	251	2	ts	ts	AFX
cord-345088-krb1eidw	251	3	mutant	mutant	NN
cord-345088-krb1eidw	251	4	quickly	quickly	RB
cord-345088-krb1eidw	251	5	lost	lose	VBD
cord-345088-krb1eidw	251	6	its	-PRON-	PRP$
cord-345088-krb1eidw	251	7	infectivity	infectivity	NN
cord-345088-krb1eidw	251	8	at	at	IN
cord-345088-krb1eidw	251	9	the	the	DT
cord-345088-krb1eidw	251	10	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	251	11	temperature	temperature	NN
cord-345088-krb1eidw	251	12	.	.	.
cord-345088-krb1eidw	252	1	This	this	DT
cord-345088-krb1eidw	252	2	low	low	JJ
cord-345088-krb1eidw	252	3	thermostability	thermostability	NN
cord-345088-krb1eidw	252	4	indicated	indicate	VBD
cord-345088-krb1eidw	252	5	that	that	IN
cord-345088-krb1eidw	252	6	even	even	RB
cord-345088-krb1eidw	252	7	if	if	IN
cord-345088-krb1eidw	252	8	the	the	DT
cord-345088-krb1eidw	252	9	mutant	mutant	JJ
cord-345088-krb1eidw	252	10	S	S	NNP
cord-345088-krb1eidw	252	11	protein	protein	NN
cord-345088-krb1eidw	252	12	was	be	VBD
cord-345088-krb1eidw	252	13	synthesized	synthesize	VBN
cord-345088-krb1eidw	252	14	,	,	,
cord-345088-krb1eidw	252	15	fully	fully	RB
cord-345088-krb1eidw	252	16	modified	modify	VBN
cord-345088-krb1eidw	252	17	and	and	CC
cord-345088-krb1eidw	252	18	assembled	assemble	VBN
cord-345088-krb1eidw	252	19	into	into	IN
cord-345088-krb1eidw	252	20	virions	virion	NNS
cord-345088-krb1eidw	252	21	at	at	IN
cord-345088-krb1eidw	252	22	the	the	DT
cord-345088-krb1eidw	252	23	permissive	permissive	JJ
cord-345088-krb1eidw	252	24	temperature	temperature	NN
cord-345088-krb1eidw	252	25	,	,	,
cord-345088-krb1eidw	252	26	the	the	DT
cord-345088-krb1eidw	252	27	resulting	result	VBG
cord-345088-krb1eidw	252	28	particles	particle	NNS
cord-345088-krb1eidw	252	29	were	be	VBD
cord-345088-krb1eidw	252	30	relatively	relatively	RB
cord-345088-krb1eidw	252	31	unstable	unstable	JJ
cord-345088-krb1eidw	252	32	and	and	CC
cord-345088-krb1eidw	252	33	lost	lose	VBD
cord-345088-krb1eidw	252	34	infectivity	infectivity	NN
cord-345088-krb1eidw	252	35	at	at	IN
cord-345088-krb1eidw	252	36	the	the	DT
cord-345088-krb1eidw	252	37	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	252	38	temperature	temperature	NN
cord-345088-krb1eidw	252	39	.	.	.
cord-345088-krb1eidw	253	1	It	-PRON-	PRP
cord-345088-krb1eidw	253	2	suggests	suggest	VBZ
cord-345088-krb1eidw	253	3	that	that	IN
cord-345088-krb1eidw	253	4	the	the	DT
cord-345088-krb1eidw	253	5	Q	Q	NNP
cord-345088-krb1eidw	253	6	294	294	CD
cord-345088-krb1eidw	253	7	residue	residue	NN
cord-345088-krb1eidw	253	8	in	in	IN
cord-345088-krb1eidw	253	9	the	the	DT
cord-345088-krb1eidw	253	10	conserved	conserve	VBN
cord-345088-krb1eidw	253	11	S1	s1	NN
cord-345088-krb1eidw	253	12	domain	domain	NN
cord-345088-krb1eidw	253	13	of	of	IN
cord-345088-krb1eidw	253	14	IBV	IBV	NNP
cord-345088-krb1eidw	253	15	may	may	MD
cord-345088-krb1eidw	253	16	also	also	RB
cord-345088-krb1eidw	253	17	play	play	VB
cord-345088-krb1eidw	253	18	an	an	DT
cord-345088-krb1eidw	253	19	essential	essential	JJ
cord-345088-krb1eidw	253	20	role	role	NN
cord-345088-krb1eidw	253	21	in	in	IN
cord-345088-krb1eidw	253	22	maintaining	maintain	VBG
cord-345088-krb1eidw	253	23	the	the	DT
cord-345088-krb1eidw	253	24	thermal	thermal	JJ
cord-345088-krb1eidw	253	25	stability	stability	NN
cord-345088-krb1eidw	253	26	of	of	IN
cord-345088-krb1eidw	253	27	the	the	DT
cord-345088-krb1eidw	253	28	spike	spike	NN
cord-345088-krb1eidw	253	29	in	in	IN
cord-345088-krb1eidw	253	30	the	the	DT
cord-345088-krb1eidw	253	31	virion	virion	NN
cord-345088-krb1eidw	253	32	,	,	,
cord-345088-krb1eidw	253	33	resembling	resemble	VBG
cord-345088-krb1eidw	253	34	the	the	DT
cord-345088-krb1eidw	253	35	S	S	NNP
cord-345088-krb1eidw	253	36	287	287	CD
cord-345088-krb1eidw	253	37	residue	residue	NN
cord-345088-krb1eidw	253	38	in	in	IN
cord-345088-krb1eidw	253	39	the	the	DT
cord-345088-krb1eidw	253	40	S1	S1	NNP
cord-345088-krb1eidw	253	41	region	region	NN
cord-345088-krb1eidw	253	42	of	of	IN
cord-345088-krb1eidw	253	43	an	an	DT
cord-345088-krb1eidw	253	44	MHV	MHV	NNP
cord-345088-krb1eidw	253	45	ts	ts	NN
cord-345088-krb1eidw	253	46	mutant	mutant	NN
cord-345088-krb1eidw	253	47	(	(	-LRB-
cord-345088-krb1eidw	253	48	Ricard	Ricard	NNP
cord-345088-krb1eidw	253	49	et	et	NNP
cord-345088-krb1eidw	253	50	al	al	NNP
cord-345088-krb1eidw	253	51	.	.	NNP
cord-345088-krb1eidw	253	52	,	,	,
cord-345088-krb1eidw	253	53	1995	1995	CD
cord-345088-krb1eidw	253	54	)	)	-RRB-
cord-345088-krb1eidw	253	55	.	.	.
cord-345088-krb1eidw	254	1	In	in	IN
cord-345088-krb1eidw	254	2	addition	addition	NN
cord-345088-krb1eidw	254	3	,	,	,
cord-345088-krb1eidw	254	4	if	if	IN
cord-345088-krb1eidw	254	5	cells	cell	NNS
cord-345088-krb1eidw	254	6	were	be	VBD
cord-345088-krb1eidw	254	7	incubated	incubate	VBN
cord-345088-krb1eidw	254	8	with	with	IN
cord-345088-krb1eidw	254	9	the	the	DT
cord-345088-krb1eidw	254	10	ts	ts	NNP
cord-345088-krb1eidw	254	11	mutants	mutant	NNS
cord-345088-krb1eidw	254	12	at	at	IN
cord-345088-krb1eidw	254	13	the	the	DT
cord-345088-krb1eidw	254	14	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	254	15	temperature	temperature	NN
cord-345088-krb1eidw	254	16	,	,	,
cord-345088-krb1eidw	254	17	viral	viral	JJ
cord-345088-krb1eidw	254	18	proteins	protein	NNS
cord-345088-krb1eidw	254	19	were	be	VBD
cord-345088-krb1eidw	254	20	hardly	hardly	RB
cord-345088-krb1eidw	254	21	detected	detect	VBN
cord-345088-krb1eidw	254	22	,	,	,
cord-345088-krb1eidw	254	23	indicating	indicate	VBG
cord-345088-krb1eidw	254	24	that	that	IN
cord-345088-krb1eidw	254	25	at	at	IN
cord-345088-krb1eidw	254	26	the	the	DT
cord-345088-krb1eidw	254	27	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	254	28	temperature	temperature	NN
cord-345088-krb1eidw	254	29	,	,	,
cord-345088-krb1eidw	254	30	the	the	DT
cord-345088-krb1eidw	254	31	ts	ts	NNP
cord-345088-krb1eidw	254	32	mutant	mutant	NN
cord-345088-krb1eidw	254	33	S	S	NNP
cord-345088-krb1eidw	254	34	protein	protein	NN
cord-345088-krb1eidw	254	35	may	may	MD
cord-345088-krb1eidw	254	36	be	be	VB
cord-345088-krb1eidw	254	37	unable	unable	JJ
cord-345088-krb1eidw	254	38	to	to	TO
cord-345088-krb1eidw	254	39	either	either	DT
cord-345088-krb1eidw	254	40	bind	bind	VB
cord-345088-krb1eidw	254	41	efficiently	efficiently	RB
cord-345088-krb1eidw	254	42	to	to	IN
cord-345088-krb1eidw	254	43	the	the	DT
cord-345088-krb1eidw	254	44	receptor	receptor	NN
cord-345088-krb1eidw	254	45	or	or	CC
cord-345088-krb1eidw	254	46	induce	induce	VB
cord-345088-krb1eidw	254	47	virus	virus	NN
cord-345088-krb1eidw	254	48	-cell	-cell	CD
cord-345088-krb1eidw	254	49	membrane	membrane	NN
cord-345088-krb1eidw	254	50	fusion	fusion	NN
cord-345088-krb1eidw	254	51	in	in	IN
cord-345088-krb1eidw	254	52	contrast	contrast	NN
cord-345088-krb1eidw	254	53	to	to	IN
cord-345088-krb1eidw	254	54	the	the	DT
cord-345088-krb1eidw	254	55	S	S	NNP
cord-345088-krb1eidw	254	56	protein	protein	NN
cord-345088-krb1eidw	254	57	of	of	IN
cord-345088-krb1eidw	254	58	the	the	DT
cord-345088-krb1eidw	254	59	revertant	revertant	NN
cord-345088-krb1eidw	254	60	and	and	CC
cord-345088-krb1eidw	254	61	wt	wt	NN
cord-345088-krb1eidw	254	62	virus	virus	NN
cord-345088-krb1eidw	254	63	.	.	.
cord-345088-krb1eidw	255	1	This	this	DT
cord-345088-krb1eidw	255	2	observation	observation	NN
cord-345088-krb1eidw	255	3	suggests	suggest	VBZ
cord-345088-krb1eidw	255	4	that	that	IN
cord-345088-krb1eidw	255	5	the	the	DT
cord-345088-krb1eidw	255	6	mutation	mutation	NN
cord-345088-krb1eidw	255	7	in	in	IN
cord-345088-krb1eidw	255	8	the	the	DT
cord-345088-krb1eidw	255	9	S	S	NNP
cord-345088-krb1eidw	255	10	protein	protein	NN
cord-345088-krb1eidw	255	11	might	may	MD
cord-345088-krb1eidw	255	12	induce	induce	VB
cord-345088-krb1eidw	255	13	conformation	conformation	NN
cord-345088-krb1eidw	255	14	changes	change	NNS
cord-345088-krb1eidw	255	15	of	of	IN
cord-345088-krb1eidw	255	16	the	the	DT
cord-345088-krb1eidw	255	17	spikes	spike	NNS
cord-345088-krb1eidw	255	18	on	on	IN
cord-345088-krb1eidw	255	19	virions	virion	NNS
cord-345088-krb1eidw	255	20	at	at	IN
cord-345088-krb1eidw	255	21	the	the	DT
cord-345088-krb1eidw	255	22	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	255	23	temperature	temperature	NN
cord-345088-krb1eidw	255	24	and	and	CC
cord-345088-krb1eidw	255	25	hamper	hamper	NN
cord-345088-krb1eidw	255	26	either	either	DT
cord-345088-krb1eidw	255	27	process	process	NN
cord-345088-krb1eidw	255	28	.	.	.
cord-345088-krb1eidw	256	1	Vero	Vero	NNP
cord-345088-krb1eidw	256	2	cells	cell	NNS
cord-345088-krb1eidw	256	3	were	be	VBD
cord-345088-krb1eidw	256	4	maintained	maintain	VBN
cord-345088-krb1eidw	256	5	in	in	IN
cord-345088-krb1eidw	256	6	complete	complete	JJ
cord-345088-krb1eidw	256	7	DMEM	DMEM	NNP
cord-345088-krb1eidw	256	8	medium	medium	NN
cord-345088-krb1eidw	256	9	(	(	-LRB-
cord-345088-krb1eidw	256	10	GIBCO	GIBCO	NNP
cord-345088-krb1eidw	256	11	BRL	BRL	NNP
cord-345088-krb1eidw	256	12	)	)	-RRB-
cord-345088-krb1eidw	256	13	,	,	,
cord-345088-krb1eidw	256	14	supplemented	supplement	VBN
cord-345088-krb1eidw	256	15	with	with	IN
cord-345088-krb1eidw	256	16	newborn	newborn	JJ
cord-345088-krb1eidw	256	17	calf	calf	NN
cord-345088-krb1eidw	256	18	serum	serum	NN
cord-345088-krb1eidw	256	19	(	(	-LRB-
cord-345088-krb1eidw	256	20	10	10	CD
cord-345088-krb1eidw	256	21	%	%	NN
cord-345088-krb1eidw	256	22	)	)	-RRB-
cord-345088-krb1eidw	256	23	,	,	,
cord-345088-krb1eidw	256	24	streptomycin	streptomycin	NNS
cord-345088-krb1eidw	256	25	(	(	-LRB-
cord-345088-krb1eidw	256	26	1000	1000	CD
cord-345088-krb1eidw	256	27	Ag	Ag	NNP
cord-345088-krb1eidw	256	28	)	)	-RRB-
cord-345088-krb1eidw	256	29	and	and	CC
cord-345088-krb1eidw	256	30	penicillin	penicillin	NN
cord-345088-krb1eidw	256	31	(	(	-LRB-
cord-345088-krb1eidw	256	32	1000	1000	CD
cord-345088-krb1eidw	256	33	units	unit	NNS
cord-345088-krb1eidw	256	34	/	/	SYM
cord-345088-krb1eidw	256	35	ml	ml	NN
cord-345088-krb1eidw	256	36	)	)	-RRB-
cord-345088-krb1eidw	256	37	.	.	.
cord-345088-krb1eidw	257	1	The	the	DT
cord-345088-krb1eidw	257	2	Beaudette	Beaudette	NNP
cord-345088-krb1eidw	257	3	stain	stain	NN
cord-345088-krb1eidw	257	4	of	of	IN
cord-345088-krb1eidw	257	5	IBV	IBV	NNP
cord-345088-krb1eidw	257	6	was	be	VBD
cord-345088-krb1eidw	257	7	purchased	purchase	VBN
cord-345088-krb1eidw	257	8	from	from	IN
cord-345088-krb1eidw	257	9	ATCC	ATCC	NNP
cord-345088-krb1eidw	257	10	and	and	CC
cord-345088-krb1eidw	257	11	propagated	propagate	VBN
cord-345088-krb1eidw	257	12	in	in	IN
cord-345088-krb1eidw	257	13	chicken	chicken	NN
cord-345088-krb1eidw	257	14	embryonated	embryonate	VBN
cord-345088-krb1eidw	257	15	eggs	egg	NNS
cord-345088-krb1eidw	257	16	for	for	IN
cord-345088-krb1eidw	257	17	three	three	CD
cord-345088-krb1eidw	257	18	passages	passage	NNS
cord-345088-krb1eidw	257	19	.	.	.
cord-345088-krb1eidw	258	1	The	the	DT
cord-345088-krb1eidw	258	2	virus	virus	NN
cord-345088-krb1eidw	258	3	was	be	VBD
cord-345088-krb1eidw	258	4	then	then	RB
cord-345088-krb1eidw	258	5	adapted	adapt	VBN
cord-345088-krb1eidw	258	6	to	to	TO
cord-345088-krb1eidw	258	7	grow	grow	VB
cord-345088-krb1eidw	258	8	and	and	CC
cord-345088-krb1eidw	258	9	passage	passage	VB
cord-345088-krb1eidw	258	10	on	on	IN
cord-345088-krb1eidw	258	11	Vero	Vero	NNP
cord-345088-krb1eidw	258	12	cells	cell	NNS
cord-345088-krb1eidw	258	13	for	for	IN
cord-345088-krb1eidw	258	14	65	65	CD
cord-345088-krb1eidw	258	15	times	time	NNS
cord-345088-krb1eidw	258	16	at	at	IN
cord-345088-krb1eidw	258	17	37	37	CD
cord-345088-krb1eidw	258	18	jC.	jc.	NN
cord-345088-krb1eidw	259	1	In	in	IN
cord-345088-krb1eidw	259	2	this	this	DT
cord-345088-krb1eidw	259	3	study	study	NN
cord-345088-krb1eidw	259	4	,	,	,
cord-345088-krb1eidw	259	5	the	the	DT
cord-345088-krb1eidw	259	6	viruses	virus	NNS
cord-345088-krb1eidw	259	7	were	be	VBD
cord-345088-krb1eidw	259	8	further	further	RB
cord-345088-krb1eidw	259	9	passaged	passage	VBN
cord-345088-krb1eidw	259	10	on	on	IN
cord-345088-krb1eidw	259	11	Vero	Vero	NNP
cord-345088-krb1eidw	259	12	cells	cell	NNS
cord-345088-krb1eidw	259	13	at	at	IN
cord-345088-krb1eidw	259	14	progressively	progressively	RB
cord-345088-krb1eidw	259	15	lower	low	JJR
cord-345088-krb1eidw	259	16	temperatures	temperature	NNS
cord-345088-krb1eidw	259	17	for	for	IN
cord-345088-krb1eidw	259	18	121	121	CD
cord-345088-krb1eidw	259	19	passages	passage	NNS
cord-345088-krb1eidw	259	20	(	(	-LRB-
cord-345088-krb1eidw	259	21	at	at	IN
cord-345088-krb1eidw	259	22	35	35	CD
cord-345088-krb1eidw	259	23	,	,	,
cord-345088-krb1eidw	259	24	34	34	CD
cord-345088-krb1eidw	259	25	,	,	,
cord-345088-krb1eidw	259	26	33	33	CD
cord-345088-krb1eidw	259	27	,	,	,
cord-345088-krb1eidw	259	28	32	32	CD
cord-345088-krb1eidw	259	29	,	,	,
cord-345088-krb1eidw	259	30	30	30	CD
cord-345088-krb1eidw	259	31	,	,	,
cord-345088-krb1eidw	259	32	29	29	CD
cord-345088-krb1eidw	259	33	,	,	,
cord-345088-krb1eidw	259	34	and	and	CC
cord-345088-krb1eidw	259	35	28	28	CD
cord-345088-krb1eidw	259	36	jC	jC	NNS
cord-345088-krb1eidw	259	37	for	for	IN
cord-345088-krb1eidw	259	38	4	4	CD
cord-345088-krb1eidw	259	39	,	,	,
cord-345088-krb1eidw	259	40	5	5	CD
cord-345088-krb1eidw	259	41	,	,	,
cord-345088-krb1eidw	259	42	7	7	CD
cord-345088-krb1eidw	259	43	,	,	,
cord-345088-krb1eidw	259	44	44	44	CD
cord-345088-krb1eidw	259	45	,	,	,
cord-345088-krb1eidw	259	46	12	12	CD
cord-345088-krb1eidw	259	47	,	,	,
cord-345088-krb1eidw	259	48	21	21	CD
cord-345088-krb1eidw	259	49	,	,	,
cord-345088-krb1eidw	259	50	and	and	CC
cord-345088-krb1eidw	259	51	29	29	CD
cord-345088-krb1eidw	259	52	passages	passage	NNS
cord-345088-krb1eidw	259	53	)	)	-RRB-
cord-345088-krb1eidw	259	54	.	.	.
cord-345088-krb1eidw	260	1	Virus	virus	NN
cord-345088-krb1eidw	260	2	stocks	stock	NNS
cord-345088-krb1eidw	260	3	were	be	VBD
cord-345088-krb1eidw	260	4	used	use	VBN
cord-345088-krb1eidw	260	5	for	for	IN
cord-345088-krb1eidw	260	6	plaque	plaque	NN
cord-345088-krb1eidw	260	7	assays	assay	NNS
cord-345088-krb1eidw	260	8	,	,	,
cord-345088-krb1eidw	260	9	plaque	plaque	NN
cord-345088-krb1eidw	260	10	-	-	HYPH
cord-345088-krb1eidw	260	11	to	to	IN
cord-345088-krb1eidw	260	12	-	-	HYPH
cord-345088-krb1eidw	260	13	plaque	plaque	NN
cord-345088-krb1eidw	260	14	purification	purification	NN
cord-345088-krb1eidw	260	15	and	and	CC
cord-345088-krb1eidw	260	16	viral	viral	JJ
cord-345088-krb1eidw	260	17	RNA	RNA	NNP
cord-345088-krb1eidw	260	18	extraction	extraction	NN
cord-345088-krb1eidw	260	19	as	as	IN
cord-345088-krb1eidw	260	20	described	describe	VBN
cord-345088-krb1eidw	260	21	previously	previously	RB
cord-345088-krb1eidw	260	22	(	(	-LRB-
cord-345088-krb1eidw	260	23	Shen	Shen	NNP
cord-345088-krb1eidw	260	24	et	et	NNP
cord-345088-krb1eidw	260	25	al	al	NNP
cord-345088-krb1eidw	260	26	.	.	NNP
cord-345088-krb1eidw	260	27	,	,	,
cord-345088-krb1eidw	260	28	1994	1994	CD
cord-345088-krb1eidw	260	29	)	)	-RRB-
cord-345088-krb1eidw	260	30	.	.	.
cord-345088-krb1eidw	261	1	The	the	DT
cord-345088-krb1eidw	261	2	wild	wild	JJ
cord-345088-krb1eidw	261	3	-	-	HYPH
cord-345088-krb1eidw	261	4	type	type	NN
cord-345088-krb1eidw	261	5	virus	virus	NN
cord-345088-krb1eidw	261	6	,	,	,
cord-345088-krb1eidw	261	7	wt6501	wt6501	NN
cord-345088-krb1eidw	261	8	,	,	,
cord-345088-krb1eidw	261	9	was	be	VBD
cord-345088-krb1eidw	261	10	plaque	plaque	NN
cord-345088-krb1eidw	261	11	-	-	HYPH
cord-345088-krb1eidw	261	12	purified	purify	VBN
cord-345088-krb1eidw	261	13	from	from	IN
cord-345088-krb1eidw	261	14	passage	passage	NN
cord-345088-krb1eidw	261	15	65	65	CD
cord-345088-krb1eidw	261	16	grown	grow	VBN
cord-345088-krb1eidw	261	17	at	at	IN
cord-345088-krb1eidw	261	18	37	37	CD
cord-345088-krb1eidw	261	19	jC	jC	NNS
cord-345088-krb1eidw	261	20	,	,	,
cord-345088-krb1eidw	261	21	while	while	IN
cord-345088-krb1eidw	261	22	ts	ts	NNP
cord-345088-krb1eidw	261	23	mutants	mutant	NNS
cord-345088-krb1eidw	261	24	,	,	,
cord-345088-krb1eidw	261	25	ts291602	ts291602	NNP
cord-345088-krb1eidw	261	26	and	and	CC
cord-345088-krb1eidw	261	27	ts282902	ts282902	XX
cord-345088-krb1eidw	261	28	,	,	,
cord-345088-krb1eidw	261	29	were	be	VBD
cord-345088-krb1eidw	261	30	purified	purify	VBN
cord-345088-krb1eidw	261	31	from	from	IN
cord-345088-krb1eidw	261	32	passage	passage	NN
cord-345088-krb1eidw	261	33	16	16	CD
cord-345088-krb1eidw	261	34	grown	grow	VBN
cord-345088-krb1eidw	261	35	at	at	IN
cord-345088-krb1eidw	261	36	29	29	CD
cord-345088-krb1eidw	261	37	jC	jC	NNP
cord-345088-krb1eidw	261	38	and	and	CC
cord-345088-krb1eidw	261	39	passage	passage	NN
cord-345088-krb1eidw	261	40	29	29	CD
cord-345088-krb1eidw	261	41	grown	grow	VBN
cord-345088-krb1eidw	261	42	at	at	IN
cord-345088-krb1eidw	261	43	28	28	CD
cord-345088-krb1eidw	261	44	jC	jC	NNP
cord-345088-krb1eidw	261	45	,	,	,
cord-345088-krb1eidw	261	46	respectively	respectively	RB
cord-345088-krb1eidw	261	47	.	.	.
cord-345088-krb1eidw	262	1	The	the	DT
cord-345088-krb1eidw	262	2	revertant	revertant	NN
cord-345088-krb1eidw	262	3	,	,	,
cord-345088-krb1eidw	262	4	rev-1	rev-1	NNP
cord-345088-krb1eidw	262	5	,	,	,
cord-345088-krb1eidw	262	6	was	be	VBD
cord-345088-krb1eidw	262	7	plaque	plaque	NN
cord-345088-krb1eidw	262	8	-	-	HYPH
cord-345088-krb1eidw	262	9	purified	purify	VBN
cord-345088-krb1eidw	262	10	from	from	IN
cord-345088-krb1eidw	262	11	cells	cell	NNS
cord-345088-krb1eidw	262	12	infected	infect	VBN
cord-345088-krb1eidw	262	13	with	with	IN
cord-345088-krb1eidw	262	14	ts	ts	NNP
cord-345088-krb1eidw	262	15	291602	291602	CD
cord-345088-krb1eidw	262	16	for	for	IN
cord-345088-krb1eidw	262	17	12	12	CD
cord-345088-krb1eidw	262	18	h	h	NN
cord-345088-krb1eidw	262	19	at	at	IN
cord-345088-krb1eidw	262	20	37	37	CD
cord-345088-krb1eidw	262	21	jC	jC	NNS
cord-345088-krb1eidw	262	22	and	and	CC
cord-345088-krb1eidw	262	23	then	then	RB
cord-345088-krb1eidw	262	24	shifted	shift	VBD
cord-345088-krb1eidw	262	25	to	to	IN
cord-345088-krb1eidw	262	26	40	40	CD
cord-345088-krb1eidw	262	27	jC	jC	NNS
cord-345088-krb1eidw	262	28	for	for	IN
cord-345088-krb1eidw	262	29	2	2	CD
cord-345088-krb1eidw	262	30	days	day	NNS
cord-345088-krb1eidw	262	31	.	.	.
cord-345088-krb1eidw	263	1	Recombinant	recombinant	JJ
cord-345088-krb1eidw	263	2	vaccinia	vaccinia	NN
cord-345088-krb1eidw	263	3	/	/	SYM
cord-345088-krb1eidw	263	4	T7	t7	NN
cord-345088-krb1eidw	263	5	(	(	-LRB-
cord-345088-krb1eidw	263	6	V	V	NNP
cord-345088-krb1eidw	263	7	/	/	SYM
cord-345088-krb1eidw	263	8	T3	T3	NNP
cord-345088-krb1eidw	263	9	)	)	-RRB-
cord-345088-krb1eidw	263	10	virus	virus	NN
cord-345088-krb1eidw	263	11	was	be	VBD
cord-345088-krb1eidw	263	12	propagated	propagate	VBN
cord-345088-krb1eidw	263	13	and	and	CC
cord-345088-krb1eidw	263	14	was	be	VBD
cord-345088-krb1eidw	263	15	tittered	titter	VBN
cord-345088-krb1eidw	263	16	on	on	IN
cord-345088-krb1eidw	263	17	Vero	Vero	NNP
cord-345088-krb1eidw	263	18	cells	cell	NNS
cord-345088-krb1eidw	263	19	.	.	.
cord-345088-krb1eidw	264	1	Virus	virus	NN
cord-345088-krb1eidw	264	2	stocks	stock	NNS
cord-345088-krb1eidw	264	3	were	be	VBD
cord-345088-krb1eidw	264	4	kept	keep	VBN
cord-345088-krb1eidw	264	5	at	at	IN
cord-345088-krb1eidw	264	6	À80	À80	NNP
cord-345088-krb1eidw	264	7	jC	jC	NNP
cord-345088-krb1eidw	264	8	until	until	IN
cord-345088-krb1eidw	264	9	use	use	NN
cord-345088-krb1eidw	264	10	.	.	.
cord-345088-krb1eidw	265	1	Confluent	confluent	NN
cord-345088-krb1eidw	265	2	monolayers	monolayer	NNS
cord-345088-krb1eidw	265	3	of	of	IN
cord-345088-krb1eidw	265	4	Vero	Vero	NNP
cord-345088-krb1eidw	265	5	cells	cell	NNS
cord-345088-krb1eidw	265	6	were	be	VBD
cord-345088-krb1eidw	265	7	infected	infect	VBN
cord-345088-krb1eidw	265	8	with	with	IN
cord-345088-krb1eidw	265	9	viruses	virus	NNS
cord-345088-krb1eidw	265	10	at	at	IN
cord-345088-krb1eidw	265	11	a	a	DT
cord-345088-krb1eidw	265	12	multiplicity	multiplicity	NN
cord-345088-krb1eidw	265	13	of	of	IN
cord-345088-krb1eidw	265	14	infection	infection	NN
cord-345088-krb1eidw	265	15	(	(	-LRB-
cord-345088-krb1eidw	265	16	MOI	MOI	NNP
cord-345088-krb1eidw	265	17	)	)	-RRB-
cord-345088-krb1eidw	265	18	of	of	IN
cord-345088-krb1eidw	265	19	0.1	0.1	CD
cord-345088-krb1eidw	265	20	.	.	.
cord-345088-krb1eidw	266	1	After	after	IN
cord-345088-krb1eidw	266	2	2h	2h	CD
cord-345088-krb1eidw	266	3	absorption	absorption	NN
cord-345088-krb1eidw	266	4	,	,	,
cord-345088-krb1eidw	266	5	the	the	DT
cord-345088-krb1eidw	266	6	viruses	virus	NNS
cord-345088-krb1eidw	266	7	were	be	VBD
cord-345088-krb1eidw	266	8	radiolabeled	radiolabele	VBN
cord-345088-krb1eidw	266	9	by	by	IN
cord-345088-krb1eidw	266	10	replacing	replace	VBG
cord-345088-krb1eidw	266	11	medium	medium	NN
cord-345088-krb1eidw	266	12	with	with	IN
cord-345088-krb1eidw	266	13	methionine	methionine	NN
cord-345088-krb1eidw	266	14	-	-	HYPH
cord-345088-krb1eidw	266	15	free	free	JJ
cord-345088-krb1eidw	266	16	DMEM	DMEM	NNP
cord-345088-krb1eidw	266	17	supplemented	supplement	VBN
cord-345088-krb1eidw	266	18	with	with	IN
cord-345088-krb1eidw	266	19	30	30	CD
cord-345088-krb1eidw	266	20	Aci	Aci	NNP
cord-345088-krb1eidw	266	21	/	/	SYM
cord-345088-krb1eidw	266	22	ml	ml	NN
cord-345088-krb1eidw	266	23	[	[	-LRB-
cord-345088-krb1eidw	266	24	35	35	CD
cord-345088-krb1eidw	266	25	S]-methionine	S]-methionine	NNS
cord-345088-krb1eidw	266	26	.	.	.
cord-345088-krb1eidw	267	1	After	after	IN
cord-345088-krb1eidw	267	2	16-h	16-h	CD
cord-345088-krb1eidw	267	3	incubation	incubation	NN
cord-345088-krb1eidw	267	4	at	at	IN
cord-345088-krb1eidw	267	5	temperatures	temperature	NNS
cord-345088-krb1eidw	267	6	appropriate	appropriate	JJ
cord-345088-krb1eidw	267	7	for	for	IN
cord-345088-krb1eidw	267	8	each	each	DT
cord-345088-krb1eidw	267	9	virus	virus	NN
cord-345088-krb1eidw	267	10	,	,	,
cord-345088-krb1eidw	267	11	cells	cell	NNS
cord-345088-krb1eidw	267	12	were	be	VBD
cord-345088-krb1eidw	267	13	harvested	harvest	VBN
cord-345088-krb1eidw	267	14	and	and	CC
cord-345088-krb1eidw	267	15	virus	virus	NN
cord-345088-krb1eidw	267	16	stock	stock	NN
cord-345088-krb1eidw	267	17	was	be	VBD
cord-345088-krb1eidw	267	18	prepared	prepare	VBN
cord-345088-krb1eidw	267	19	by	by	IN
cord-345088-krb1eidw	267	20	freezing	freeze	VBG
cord-345088-krb1eidw	267	21	and	and	CC
cord-345088-krb1eidw	267	22	thawing	thaw	VBG
cord-345088-krb1eidw	267	23	three	three	CD
cord-345088-krb1eidw	267	24	times	time	NNS
cord-345088-krb1eidw	267	25	.	.	.
cord-345088-krb1eidw	268	1	Cell	cell	NN
cord-345088-krb1eidw	268	2	debris	debris	NN
cord-345088-krb1eidw	268	3	was	be	VBD
cord-345088-krb1eidw	268	4	removed	remove	VBN
cord-345088-krb1eidw	268	5	by	by	IN
cord-345088-krb1eidw	268	6	centrifugation	centrifugation	NN
cord-345088-krb1eidw	268	7	at	at	IN
cord-345088-krb1eidw	268	8	5000	5000	CD
cord-345088-krb1eidw	268	9	rpm	rpm	NN
cord-345088-krb1eidw	268	10	for	for	IN
cord-345088-krb1eidw	268	11	15	15	CD
cord-345088-krb1eidw	268	12	min	min	NN
cord-345088-krb1eidw	268	13	(	(	-LRB-
cord-345088-krb1eidw	268	14	Beckmen	Beckmen	NNP
cord-345088-krb1eidw	268	15	,	,	,
cord-345088-krb1eidw	268	16	25.50	25.50	CD
cord-345088-krb1eidw	268	17	)	)	-RRB-
cord-345088-krb1eidw	268	18	.	.	.
cord-345088-krb1eidw	269	1	The	the	DT
cord-345088-krb1eidw	269	2	supernatant	supernatant	NN
cord-345088-krb1eidw	269	3	was	be	VBD
cord-345088-krb1eidw	269	4	centrifuged	centrifuge	VBN
cord-345088-krb1eidw	269	5	through	through	IN
cord-345088-krb1eidw	269	6	a	a	DT
cord-345088-krb1eidw	269	7	20	20	CD
cord-345088-krb1eidw	269	8	%	%	NN
cord-345088-krb1eidw	269	9	sucrose	sucrose	NN
cord-345088-krb1eidw	269	10	cushion	cushion	NN
cord-345088-krb1eidw	269	11	and	and	CC
cord-345088-krb1eidw	269	12	the	the	DT
cord-345088-krb1eidw	269	13	resulting	result	VBG
cord-345088-krb1eidw	269	14	pellet	pellet	NN
cord-345088-krb1eidw	269	15	was	be	VBD
cord-345088-krb1eidw	269	16	resuspended	resuspend	VBN
cord-345088-krb1eidw	269	17	in	in	IN
cord-345088-krb1eidw	269	18	TNE	TNE	NNP
cord-345088-krb1eidw	269	19	buffer	buffer	NN
cord-345088-krb1eidw	269	20	(	(	-LRB-
cord-345088-krb1eidw	269	21	50	50	CD
cord-345088-krb1eidw	269	22	mM	mm	CD
cord-345088-krb1eidw	269	23	Tris	Tris	NNP
cord-345088-krb1eidw	269	24	-HCl	-HCl	''
cord-345088-krb1eidw	269	25	,	,	,
cord-345088-krb1eidw	269	26	pH	pH	NNP
cord-345088-krb1eidw	269	27	7.4	7.4	CD
cord-345088-krb1eidw	269	28	,	,	,
cord-345088-krb1eidw	269	29	100	100	CD
cord-345088-krb1eidw	269	30	mM	mm	CD
cord-345088-krb1eidw	269	31	NaCl	nacl	NN
cord-345088-krb1eidw	269	32	,	,	,
cord-345088-krb1eidw	269	33	1	1	CD
cord-345088-krb1eidw	269	34	mM	mm	CD
cord-345088-krb1eidw	269	35	EDTA	EDTA	NNP
cord-345088-krb1eidw	269	36	)	)	-RRB-
cord-345088-krb1eidw	269	37	.	.	.
cord-345088-krb1eidw	270	1	The	the	DT
cord-345088-krb1eidw	270	2	viruses	virus	NNS
cord-345088-krb1eidw	270	3	were	be	VBD
cord-345088-krb1eidw	270	4	further	further	RB
cord-345088-krb1eidw	270	5	purified	purify	VBN
cord-345088-krb1eidw	270	6	by	by	IN
cord-345088-krb1eidw	270	7	centrifugation	centrifugation	NN
cord-345088-krb1eidw	270	8	through	through	IN
cord-345088-krb1eidw	270	9	a	a	DT
cord-345088-krb1eidw	270	10	20	20	CD
cord-345088-krb1eidw	270	11	-	-	SYM
cord-345088-krb1eidw	270	12	55	55	CD
cord-345088-krb1eidw	270	13	%	%	NN
cord-345088-krb1eidw	270	14	su	su	NNP
cord-345088-krb1eidw	270	15	-	-	HYPH
cord-345088-krb1eidw	270	16	crose	crose	JJ
cord-345088-krb1eidw	270	17	gradient	gradient	NN
cord-345088-krb1eidw	270	18	in	in	IN
cord-345088-krb1eidw	270	19	TNE	TNE	NNP
cord-345088-krb1eidw	270	20	buffer	buffer	NN
cord-345088-krb1eidw	270	21	at	at	IN
cord-345088-krb1eidw	270	22	45,000	45,000	CD
cord-345088-krb1eidw	270	23	rpm	rpm	NN
cord-345088-krb1eidw	270	24	(	(	-LRB-
cord-345088-krb1eidw	270	25	Beckmen	Beckmen	NNP
cord-345088-krb1eidw	270	26	,	,	,
cord-345088-krb1eidw	270	27	SW50	SW50	NNP
cord-345088-krb1eidw	270	28	)	)	-RRB-
cord-345088-krb1eidw	270	29	twice	twice	RB
cord-345088-krb1eidw	270	30	.	.	.
cord-345088-krb1eidw	271	1	Fractions	fraction	NNS
cord-345088-krb1eidw	271	2	containing	contain	VBG
cord-345088-krb1eidw	271	3	the	the	DT
cord-345088-krb1eidw	271	4	virus	virus	NN
cord-345088-krb1eidw	271	5	were	be	VBD
cord-345088-krb1eidw	271	6	pooled	pool	VBN
cord-345088-krb1eidw	271	7	together	together	RB
cord-345088-krb1eidw	271	8	and	and	CC
cord-345088-krb1eidw	271	9	used	use	VBN
cord-345088-krb1eidw	271	10	for	for	IN
cord-345088-krb1eidw	271	11	protein	protein	NN
cord-345088-krb1eidw	271	12	analysis	analysis	NN
cord-345088-krb1eidw	271	13	.	.	.
cord-345088-krb1eidw	272	1	Viral	viral	JJ
cord-345088-krb1eidw	272	2	structural	structural	JJ
cord-345088-krb1eidw	272	3	proteins	protein	NNS
cord-345088-krb1eidw	272	4	were	be	VBD
cord-345088-krb1eidw	272	5	labeled	label	VBN
cord-345088-krb1eidw	272	6	with	with	IN
cord-345088-krb1eidw	272	7	[	[	-LRB-
cord-345088-krb1eidw	272	8	35	35	CD
cord-345088-krb1eidw	272	9	S]-methionine	S]-methionine	NNP
cord-345088-krb1eidw	272	10	,	,	,
cord-345088-krb1eidw	272	11	immunoprecipitated	immunoprecipitated	JJ
cord-345088-krb1eidw	272	12	and	and	CC
cord-345088-krb1eidw	272	13	separated	separate	VBD
cord-345088-krb1eidw	272	14	on	on	IN
cord-345088-krb1eidw	272	15	12.5	12.5	CD
cord-345088-krb1eidw	272	16	%	%	NN
cord-345088-krb1eidw	272	17	polyacrylamide	polyacrylamide	NN
cord-345088-krb1eidw	272	18	gels	gel	NNS
cord-345088-krb1eidw	272	19	as	as	IN
cord-345088-krb1eidw	272	20	described	describe	VBN
cord-345088-krb1eidw	272	21	previously	previously	RB
cord-345088-krb1eidw	272	22	(	(	-LRB-
cord-345088-krb1eidw	272	23	Liu	Liu	NNP
cord-345088-krb1eidw	272	24	et	et	NNP
cord-345088-krb1eidw	272	25	al	al	NNP
cord-345088-krb1eidw	272	26	.	.	NNP
cord-345088-krb1eidw	272	27	,	,	,
cord-345088-krb1eidw	272	28	1998	1998	CD
cord-345088-krb1eidw	272	29	)	)	-RRB-
cord-345088-krb1eidw	272	30	.	.	.
cord-345088-krb1eidw	273	1	The	the	DT
cord-345088-krb1eidw	273	2	anti	anti	JJ
cord-345088-krb1eidw	273	3	-	-	JJ
cord-345088-krb1eidw	273	4	IBV	IBV	NNP
cord-345088-krb1eidw	273	5	serum	serum	NN
cord-345088-krb1eidw	273	6	was	be	VBD
cord-345088-krb1eidw	273	7	raised	raise	VBN
cord-345088-krb1eidw	273	8	in	in	IN
cord-345088-krb1eidw	273	9	rabbit	rabbit	NN
cord-345088-krb1eidw	273	10	against	against	IN
cord-345088-krb1eidw	273	11	purified	purify	VBN
cord-345088-krb1eidw	273	12	IBV	IBV	NNP
cord-345088-krb1eidw	273	13	virions	virion	NNS
cord-345088-krb1eidw	273	14	and	and	CC
cord-345088-krb1eidw	273	15	was	be	VBD
cord-345088-krb1eidw	273	16	used	use	VBN
cord-345088-krb1eidw	273	17	in	in	IN
cord-345088-krb1eidw	273	18	immunoprecipitation	immunoprecipitation	NN
cord-345088-krb1eidw	273	19	and	and	CC
cord-345088-krb1eidw	273	20	Western	western	JJ
cord-345088-krb1eidw	273	21	blot	blot	NN
cord-345088-krb1eidw	273	22	as	as	IN
cord-345088-krb1eidw	273	23	previously	previously	RB
cord-345088-krb1eidw	273	24	described	describe	VBN
cord-345088-krb1eidw	273	25	(	(	-LRB-
cord-345088-krb1eidw	273	26	Liu	Liu	NNP
cord-345088-krb1eidw	273	27	and	and	CC
cord-345088-krb1eidw	273	28	Inglis	Inglis	NNP
cord-345088-krb1eidw	273	29	,	,	,
cord-345088-krb1eidw	273	30	1991	1991	CD
cord-345088-krb1eidw	273	31	)	)	-RRB-
cord-345088-krb1eidw	273	32	.	.	.
cord-345088-krb1eidw	274	1	A	a	DT
cord-345088-krb1eidw	274	2	portion	portion	NN
cord-345088-krb1eidw	274	3	of	of	IN
cord-345088-krb1eidw	274	4	cell	cell	NN
cord-345088-krb1eidw	274	5	lysates	lysate	NNS
cord-345088-krb1eidw	274	6	was	be	VBD
cord-345088-krb1eidw	274	7	immunoprecipitated	immunoprecipitate	VBN
cord-345088-krb1eidw	274	8	with	with	IN
cord-345088-krb1eidw	274	9	anti	anti	JJ
cord-345088-krb1eidw	274	10	-	-	JJ
cord-345088-krb1eidw	274	11	IBV	IBV	NNP
cord-345088-krb1eidw	274	12	serum	serum	NN
cord-345088-krb1eidw	274	13	.	.	.
cord-345088-krb1eidw	275	1	The	the	DT
cord-345088-krb1eidw	275	2	pellets	pellet	NNS
cord-345088-krb1eidw	275	3	were	be	VBD
cord-345088-krb1eidw	275	4	washed	wash	VBN
cord-345088-krb1eidw	275	5	three	three	CD
cord-345088-krb1eidw	275	6	times	time	NNS
cord-345088-krb1eidw	275	7	with	with	IN
cord-345088-krb1eidw	275	8	standard	standard	JJ
cord-345088-krb1eidw	275	9	RIPA	RIPA	NNP
cord-345088-krb1eidw	275	10	buffer	buffer	NN
cord-345088-krb1eidw	275	11	,	,	,
cord-345088-krb1eidw	275	12	dissolved	dissolve	VBN
cord-345088-krb1eidw	275	13	in	in	IN
cord-345088-krb1eidw	275	14	20	20	CD
cord-345088-krb1eidw	275	15	Al	Al	NNP
cord-345088-krb1eidw	275	16	of	of	IN
cord-345088-krb1eidw	275	17	digestion	digestion	NN
cord-345088-krb1eidw	275	18	buffer	buffer	NN
cord-345088-krb1eidw	275	19	(	(	-LRB-
cord-345088-krb1eidw	275	20	50	50	CD
cord-345088-krb1eidw	275	21	mM	mm	CD
cord-345088-krb1eidw	275	22	Tris	Tris	NNP
cord-345088-krb1eidw	275	23	,	,	,
cord-345088-krb1eidw	275	24	pH	pH	NNP
cord-345088-krb1eidw	275	25	6.8	6.8	CD
cord-345088-krb1eidw	275	26	,	,	,
cord-345088-krb1eidw	275	27	0.25	0.25	CD
cord-345088-krb1eidw	275	28	%	%	NN
cord-345088-krb1eidw	275	29	SDS	SDS	NNP
cord-345088-krb1eidw	275	30	)	)	-RRB-
cord-345088-krb1eidw	275	31	and	and	CC
cord-345088-krb1eidw	275	32	incubated	incubate	VBN
cord-345088-krb1eidw	275	33	at	at	IN
cord-345088-krb1eidw	275	34	95	95	CD
cord-345088-krb1eidw	275	35	jC	jC	NNS
cord-345088-krb1eidw	275	36	for	for	IN
cord-345088-krb1eidw	275	37	5	5	CD
cord-345088-krb1eidw	275	38	min	min	NN
cord-345088-krb1eidw	275	39	.	.	.
cord-345088-krb1eidw	276	1	Ten	ten	CD
cord-345088-krb1eidw	276	2	microliters	microliter	NNS
cord-345088-krb1eidw	276	3	of	of	IN
cord-345088-krb1eidw	276	4	the	the	DT
cord-345088-krb1eidw	276	5	supernatants	supernatant	NNS
cord-345088-krb1eidw	276	6	were	be	VBD
cord-345088-krb1eidw	276	7	mixed	mix	VBN
cord-345088-krb1eidw	276	8	with	with	IN
cord-345088-krb1eidw	276	9	10	10	CD
cord-345088-krb1eidw	276	10	Al	Al	NNP
cord-345088-krb1eidw	276	11	of	of	IN
cord-345088-krb1eidw	276	12	digestion	digestion	NN
cord-345088-krb1eidw	276	13	buffer	buffer	NN
cord-345088-krb1eidw	276	14	with	with	IN
cord-345088-krb1eidw	276	15	or	or	CC
cord-345088-krb1eidw	276	16	without	without	IN
cord-345088-krb1eidw	276	17	Endo	endo	NN
cord-345088-krb1eidw	276	18	-	-	HYPH
cord-345088-krb1eidw	276	19	H	H	NNP
cord-345088-krb1eidw	276	20	(	(	-LRB-
cord-345088-krb1eidw	276	21	0.2	0.2	CD
cord-345088-krb1eidw	276	22	mU	mU	NNS
cord-345088-krb1eidw	276	23	,	,	,
cord-345088-krb1eidw	276	24	Boehringer	Boehringer	NNP
cord-345088-krb1eidw	276	25	Mannheim	Mannheim	NNP
cord-345088-krb1eidw	276	26	)	)	-RRB-
cord-345088-krb1eidw	276	27	and	and	CC
cord-345088-krb1eidw	276	28	incubated	incubate	VBN
cord-345088-krb1eidw	276	29	for	for	IN
cord-345088-krb1eidw	276	30	4	4	CD
cord-345088-krb1eidw	276	31	h	h	NN
cord-345088-krb1eidw	276	32	at	at	IN
cord-345088-krb1eidw	276	33	37	37	CD
cord-345088-krb1eidw	276	34	jC.	jC.	NNP
cord-345088-krb1eidw	276	35	Confluent	Confluent	NNP
cord-345088-krb1eidw	276	36	monolayers	monolayer	NNS
cord-345088-krb1eidw	276	37	of	of	IN
cord-345088-krb1eidw	276	38	Vero	Vero	NNP
cord-345088-krb1eidw	276	39	cells	cell	NNS
cord-345088-krb1eidw	276	40	were	be	VBD
cord-345088-krb1eidw	276	41	infected	infect	VBN
cord-345088-krb1eidw	276	42	with	with	IN
cord-345088-krb1eidw	276	43	recombinant	recombinant	JJ
cord-345088-krb1eidw	276	44	vaccinia	vaccinia	NN
cord-345088-krb1eidw	276	45	/	/	SYM
cord-345088-krb1eidw	276	46	T7	t7	NN
cord-345088-krb1eidw	276	47	viruses	virus	NNS
cord-345088-krb1eidw	276	48	at	at	IN
cord-345088-krb1eidw	276	49	a	a	DT
cord-345088-krb1eidw	276	50	MOI	moi	NN
cord-345088-krb1eidw	276	51	of	of	IN
cord-345088-krb1eidw	276	52	0.1	0.1	CD
cord-345088-krb1eidw	276	53	.	.	.
cord-345088-krb1eidw	277	1	After	after	IN
cord-345088-krb1eidw	277	2	1h	1h	CD
cord-345088-krb1eidw	277	3	absorption	absorption	NN
cord-345088-krb1eidw	277	4	,	,	,
cord-345088-krb1eidw	277	5	cells	cell	NNS
cord-345088-krb1eidw	277	6	(	(	-LRB-
cord-345088-krb1eidw	277	7	2	2	CD
cord-345088-krb1eidw	277	8	Â	â	CD
cord-345088-krb1eidw	277	9	10	10	CD
cord-345088-krb1eidw	277	10	7	7	CD
cord-345088-krb1eidw	277	11	)	)	-RRB-
cord-345088-krb1eidw	277	12	were	be	VBD
cord-345088-krb1eidw	277	13	trypsinized	trypsinize	VBN
cord-345088-krb1eidw	277	14	,	,	,
cord-345088-krb1eidw	277	15	centrifuged	centrifuge	VBN
cord-345088-krb1eidw	277	16	at	at	IN
cord-345088-krb1eidw	277	17	250	250	CD
cord-345088-krb1eidw	277	18	g	g	NN
cord-345088-krb1eidw	277	19	and	and	CC
cord-345088-krb1eidw	277	20	resuspended	resuspend	VBD
cord-345088-krb1eidw	277	21	in	in	IN
cord-345088-krb1eidw	277	22	2	2	CD
cord-345088-krb1eidw	277	23	ml	ml	NNS
cord-345088-krb1eidw	277	24	of	of	IN
cord-345088-krb1eidw	277	25	PBS	PBS	NNP
cord-345088-krb1eidw	277	26	.	.	.
cord-345088-krb1eidw	278	1	The	the	DT
cord-345088-krb1eidw	278	2	cells	cell	NNS
cord-345088-krb1eidw	278	3	(	(	-LRB-
cord-345088-krb1eidw	278	4	0.3	0.3	CD
cord-345088-krb1eidw	278	5	ml	ml	NN
cord-345088-krb1eidw	278	6	)	)	-RRB-
cord-345088-krb1eidw	278	7	were	be	VBD
cord-345088-krb1eidw	278	8	mixed	mix	VBN
cord-345088-krb1eidw	278	9	with	with	IN
cord-345088-krb1eidw	278	10	5	5	CD
cord-345088-krb1eidw	278	11	Ag	Ag	NNP
cord-345088-krb1eidw	278	12	plasmid	plasmid	NN
cord-345088-krb1eidw	278	13	and	and	CC
cord-345088-krb1eidw	278	14	electroporated	electroporate	VBN
cord-345088-krb1eidw	278	15	(	(	-LRB-
cord-345088-krb1eidw	278	16	Easyject	Easyject	NNP
cord-345088-krb1eidw	278	17	,	,	,
cord-345088-krb1eidw	278	18	EquiBio	EquiBio	NNP
cord-345088-krb1eidw	278	19	)	)	-RRB-
cord-345088-krb1eidw	278	20	at	at	IN
cord-345088-krb1eidw	278	21	600	600	CD
cord-345088-krb1eidw	278	22	V	v	NN
cord-345088-krb1eidw	278	23	in	in	IN
cord-345088-krb1eidw	278	24	a	a	DT
cord-345088-krb1eidw	278	25	0.4-cm	0.4-cm	CD
cord-345088-krb1eidw	278	26	cuvette	cuvette	NN
cord-345088-krb1eidw	278	27	(	(	-LRB-
cord-345088-krb1eidw	278	28	BioRad	BioRad	NNP
cord-345088-krb1eidw	278	29	)	)	-RRB-
cord-345088-krb1eidw	278	30	.	.	.
cord-345088-krb1eidw	279	1	The	the	DT
cord-345088-krb1eidw	279	2	cells	cell	NNS
cord-345088-krb1eidw	279	3	were	be	VBD
cord-345088-krb1eidw	279	4	then	then	RB
cord-345088-krb1eidw	279	5	plated	plate	VBN
cord-345088-krb1eidw	279	6	in	in	IN
cord-345088-krb1eidw	279	7	a	a	DT
cord-345088-krb1eidw	279	8	35-mm	35-mm	CD
cord-345088-krb1eidw	279	9	dish	dish	NN
cord-345088-krb1eidw	279	10	containing	contain	VBG
cord-345088-krb1eidw	279	11	3	3	CD
cord-345088-krb1eidw	279	12	ml	ml	NNS
cord-345088-krb1eidw	279	13	of	of	IN
cord-345088-krb1eidw	279	14	DMEM	DMEM	NNP
cord-345088-krb1eidw	279	15	with	with	IN
cord-345088-krb1eidw	279	16	2	2	CD
cord-345088-krb1eidw	279	17	%	%	NN
cord-345088-krb1eidw	279	18	of	of	IN
cord-345088-krb1eidw	279	19	newborn	newborn	JJ
cord-345088-krb1eidw	279	20	calf	calf	NN
cord-345088-krb1eidw	279	21	serum	serum	NN
cord-345088-krb1eidw	279	22	and	and	CC
cord-345088-krb1eidw	279	23	incubated	incubate	VBN
cord-345088-krb1eidw	279	24	at	at	IN
cord-345088-krb1eidw	279	25	temperatures	temperature	NNS
cord-345088-krb1eidw	279	26	indicated	indicate	VBN
cord-345088-krb1eidw	279	27	.	.	.
cord-345088-krb1eidw	280	1	Viral	Viral	NNP
cord-345088-krb1eidw	280	2	RNA	RNA	NNP
cord-345088-krb1eidw	280	3	was	be	VBD
cord-345088-krb1eidw	280	4	extracted	extract	VBN
cord-345088-krb1eidw	280	5	from	from	IN
cord-345088-krb1eidw	280	6	the	the	DT
cord-345088-krb1eidw	280	7	purified	purify	VBN
cord-345088-krb1eidw	280	8	viruses	virus	NNS
cord-345088-krb1eidw	280	9	using	use	VBG
cord-345088-krb1eidw	280	10	the	the	DT
cord-345088-krb1eidw	280	11	RNeasy	RNeasy	NNP
cord-345088-krb1eidw	280	12	Mini	Mini	NNP
cord-345088-krb1eidw	280	13	Kit	kit	NN
cord-345088-krb1eidw	280	14	(	(	-LRB-
cord-345088-krb1eidw	280	15	Qiagen	Qiagen	NNP
cord-345088-krb1eidw	280	16	)	)	-RRB-
cord-345088-krb1eidw	280	17	according	accord	VBG
cord-345088-krb1eidw	280	18	to	to	IN
cord-345088-krb1eidw	280	19	the	the	DT
cord-345088-krb1eidw	280	20	manufacturer	manufacturer	NN
cord-345088-krb1eidw	280	21	's	's	POS
cord-345088-krb1eidw	280	22	instructions	instruction	NNS
cord-345088-krb1eidw	280	23	.	.	.
cord-345088-krb1eidw	281	1	Reverse	reverse	JJ
cord-345088-krb1eidw	281	2	transcription	transcription	NN
cord-345088-krb1eidw	281	3	and	and	CC
cord-345088-krb1eidw	281	4	polymerase	polymerase	NN
cord-345088-krb1eidw	281	5	chain	chain	NN
cord-345088-krb1eidw	281	6	reaction	reaction	NN
cord-345088-krb1eidw	281	7	(	(	-LRB-
cord-345088-krb1eidw	281	8	RT	RT	NNP
cord-345088-krb1eidw	281	9	-	-	HYPH
cord-345088-krb1eidw	281	10	PCR	PCR	NNP
cord-345088-krb1eidw	281	11	)	)	-RRB-
cord-345088-krb1eidw	281	12	were	be	VBD
cord-345088-krb1eidw	281	13	performed	perform	VBN
cord-345088-krb1eidw	281	14	using	use	VBG
cord-345088-krb1eidw	281	15	the	the	DT
cord-345088-krb1eidw	281	16	Expand	Expand	NNP
cord-345088-krb1eidw	281	17	Reverse	Reverse	NNP
cord-345088-krb1eidw	281	18	Transcription	Transcription	NNP
cord-345088-krb1eidw	281	19	and	and	CC
cord-345088-krb1eidw	281	20	High	High	NNP
cord-345088-krb1eidw	281	21	Fidelity	Fidelity	NNP
cord-345088-krb1eidw	281	22	PCR	PCR	NNP
cord-345088-krb1eidw	281	23	Kits	kit	NNS
cord-345088-krb1eidw	281	24	(	(	-LRB-
cord-345088-krb1eidw	281	25	Boehringer	Boehringer	NNP
cord-345088-krb1eidw	281	26	Mannheim	Mannheim	NNP
cord-345088-krb1eidw	281	27	)	)	-RRB-
cord-345088-krb1eidw	281	28	.	.	.
cord-345088-krb1eidw	282	1	Annealing	annealing	NN
cord-345088-krb1eidw	282	2	and	and	CC
cord-345088-krb1eidw	282	3	extension	extension	NN
cord-345088-krb1eidw	282	4	times	time	NNS
cord-345088-krb1eidw	282	5	of	of	IN
cord-345088-krb1eidw	282	6	PCR	PCR	NNP
cord-345088-krb1eidw	282	7	were	be	VBD
cord-345088-krb1eidw	282	8	optimized	optimize	VBN
cord-345088-krb1eidw	282	9	for	for	IN
cord-345088-krb1eidw	282	10	amplification	amplification	NN
cord-345088-krb1eidw	282	11	of	of	IN
cord-345088-krb1eidw	282	12	PCR	PCR	NNP
cord-345088-krb1eidw	282	13	products	product	NNS
cord-345088-krb1eidw	282	14	with	with	IN
cord-345088-krb1eidw	282	15	different	different	JJ
cord-345088-krb1eidw	282	16	sizes	size	NNS
cord-345088-krb1eidw	282	17	using	use	VBG
cord-345088-krb1eidw	282	18	different	different	JJ
cord-345088-krb1eidw	282	19	primers	primer	NNS
cord-345088-krb1eidw	282	20	.	.	.
cord-345088-krb1eidw	283	1	More	More	JJR
cord-345088-krb1eidw	283	2	than	than	IN
cord-345088-krb1eidw	283	3	100	100	CD
cord-345088-krb1eidw	283	4	specific	specific	JJ
cord-345088-krb1eidw	283	5	primers	primer	NNS
cord-345088-krb1eidw	283	6	were	be	VBD
cord-345088-krb1eidw	283	7	used	use	VBN
cord-345088-krb1eidw	283	8	for	for	IN
cord-345088-krb1eidw	283	9	amplification	amplification	NN
cord-345088-krb1eidw	283	10	,	,	,
cord-345088-krb1eidw	283	11	sequencing	sequencing	NN
cord-345088-krb1eidw	283	12	and	and	CC
cord-345088-krb1eidw	283	13	cloning	cloning	NN
cord-345088-krb1eidw	283	14	.	.	.
cord-345088-krb1eidw	284	1	Automated	automated	JJ
cord-345088-krb1eidw	284	2	sequencing	sequencing	NN
cord-345088-krb1eidw	284	3	was	be	VBD
cord-345088-krb1eidw	284	4	carried	carry	VBN
cord-345088-krb1eidw	284	5	out	out	RP
cord-345088-krb1eidw	284	6	using	use	VBG
cord-345088-krb1eidw	284	7	PCR	PCR	NNP
cord-345088-krb1eidw	284	8	products	product	NNS
cord-345088-krb1eidw	284	9	or	or	CC
cord-345088-krb1eidw	284	10	cDNA	cdna	NN
cord-345088-krb1eidw	284	11	clones	clone	NNS
cord-345088-krb1eidw	284	12	and	and	CC
cord-345088-krb1eidw	284	13	specific	specific	JJ
cord-345088-krb1eidw	284	14	primers	primer	NNS
cord-345088-krb1eidw	284	15	as	as	IN
cord-345088-krb1eidw	284	16	previously	previously	RB
cord-345088-krb1eidw	284	17	described	describe	VBN
cord-345088-krb1eidw	284	18	(	(	-LRB-
cord-345088-krb1eidw	284	19	Shen	Shen	NNP
cord-345088-krb1eidw	284	20	et	et	NNP
cord-345088-krb1eidw	284	21	al	al	NNP
cord-345088-krb1eidw	284	22	.	.	NNP
cord-345088-krb1eidw	284	23	,	,	,
cord-345088-krb1eidw	284	24	1999	1999	CD
cord-345088-krb1eidw	284	25	)	)	-RRB-
cord-345088-krb1eidw	284	26	.	.	.
cord-345088-krb1eidw	285	1	Sequence	sequence	NN
cord-345088-krb1eidw	285	2	analysis	analysis	NN
cord-345088-krb1eidw	285	3	was	be	VBD
cord-345088-krb1eidw	285	4	carried	carry	VBN
cord-345088-krb1eidw	285	5	out	out	RP
cord-345088-krb1eidw	285	6	using	use	VBG
cord-345088-krb1eidw	285	7	the	the	DT
cord-345088-krb1eidw	285	8	GCG	GCG	NNP
cord-345088-krb1eidw	285	9	and	and	CC
cord-345088-krb1eidw	285	10	Blast	Blast	NNP
cord-345088-krb1eidw	285	11	suite	suite	NN
cord-345088-krb1eidw	285	12	of	of	IN
cord-345088-krb1eidw	285	13	programs	program	NNS
cord-345088-krb1eidw	285	14	as	as	IN
cord-345088-krb1eidw	285	15	described	describe	VBN
cord-345088-krb1eidw	285	16	previously	previously	RB
cord-345088-krb1eidw	285	17	(	(	-LRB-
cord-345088-krb1eidw	285	18	Shen	Shen	NNP
cord-345088-krb1eidw	285	19	and	and	CC
cord-345088-krb1eidw	285	20	Liu	Liu	NNP
cord-345088-krb1eidw	285	21	,	,	,
cord-345088-krb1eidw	285	22	2000	2000	CD
cord-345088-krb1eidw	285	23	)	)	-RRB-
cord-345088-krb1eidw	285	24	.	.	.
cord-345088-krb1eidw	286	1	HeLa	HeLa	NNP
cord-345088-krb1eidw	286	2	cells	cell	NNS
cord-345088-krb1eidw	286	3	were	be	VBD
cord-345088-krb1eidw	286	4	infected	infect	VBN
cord-345088-krb1eidw	286	5	with	with	IN
cord-345088-krb1eidw	286	6	vaccinia	vaccinia	NN
cord-345088-krb1eidw	286	7	/	/	SYM
cord-345088-krb1eidw	286	8	T7	t7	NN
cord-345088-krb1eidw	286	9	virus	virus	NN
cord-345088-krb1eidw	286	10	,	,	,
cord-345088-krb1eidw	286	11	transfected	transfecte	VBN
cord-345088-krb1eidw	286	12	with	with	IN
cord-345088-krb1eidw	286	13	constructs	construct	NNS
cord-345088-krb1eidw	286	14	encoding	encode	VBG
cord-345088-krb1eidw	286	15	wt	wt	NN
cord-345088-krb1eidw	286	16	and	and	CC
cord-345088-krb1eidw	286	17	ts	ts	NNP
cord-345088-krb1eidw	286	18	mutant	mutant	NN
cord-345088-krb1eidw	286	19	S	S	NNP
cord-345088-krb1eidw	286	20	protein	protein	NN
cord-345088-krb1eidw	286	21	using	use	VBG
cord-345088-krb1eidw	286	22	QIAGEN	QIAGEN	NNP
cord-345088-krb1eidw	286	23	effectene	effectene	JJ
cord-345088-krb1eidw	286	24	transfection	transfection	NN
cord-345088-krb1eidw	286	25	reagent	reagent	NN
cord-345088-krb1eidw	286	26	,	,	,
cord-345088-krb1eidw	286	27	and	and	CC
cord-345088-krb1eidw	286	28	incubated	incubate	VBN
cord-345088-krb1eidw	286	29	at	at	IN
cord-345088-krb1eidw	286	30	the	the	DT
cord-345088-krb1eidw	286	31	permissive	permissive	JJ
cord-345088-krb1eidw	286	32	and	and	CC
cord-345088-krb1eidw	286	33	nonpermissive	nonpermissive	JJ
cord-345088-krb1eidw	286	34	temperatures	temperature	NNS
cord-345088-krb1eidw	286	35	,	,	,
cord-345088-krb1eidw	286	36	respectively	respectively	RB
cord-345088-krb1eidw	286	37	,	,	,
cord-345088-krb1eidw	286	38	for	for	IN
cord-345088-krb1eidw	286	39	18	18	CD
cord-345088-krb1eidw	286	40	h.	h.	NN
cord-345088-krb1eidw	286	41	Cells	cell	NNS
cord-345088-krb1eidw	286	42	were	be	VBD
cord-345088-krb1eidw	286	43	harvested	harvest	VBN
cord-345088-krb1eidw	286	44	,	,	,
cord-345088-krb1eidw	286	45	washed	wash	VBN
cord-345088-krb1eidw	286	46	once	once	RB
cord-345088-krb1eidw	286	47	with	with	IN
cord-345088-krb1eidw	286	48	PBS	PBS	NNP
cord-345088-krb1eidw	286	49	,	,	,
cord-345088-krb1eidw	286	50	resuspended	resuspend	VBD
cord-345088-krb1eidw	286	51	in	in	IN
cord-345088-krb1eidw	286	52	blocking	block	VBG
cord-345088-krb1eidw	286	53	buffer	buffer	NN
cord-345088-krb1eidw	286	54	(	(	-LRB-
cord-345088-krb1eidw	286	55	20	20	CD
cord-345088-krb1eidw	286	56	%	%	NN
cord-345088-krb1eidw	286	57	FBS	FBS	NNP
cord-345088-krb1eidw	286	58	and	and	CC
cord-345088-krb1eidw	286	59	1	1	CD
cord-345088-krb1eidw	286	60	%	%	NN
cord-345088-krb1eidw	286	61	BSA	BSA	NNP
cord-345088-krb1eidw	286	62	in	in	IN
cord-345088-krb1eidw	286	63	PBS	PBS	NNP
cord-345088-krb1eidw	286	64	)	)	-RRB-
cord-345088-krb1eidw	286	65	,	,	,
cord-345088-krb1eidw	286	66	incubated	incubate	VBN
cord-345088-krb1eidw	286	67	on	on	IN
cord-345088-krb1eidw	286	68	ice	ice	NN
cord-345088-krb1eidw	286	69	for	for	IN
cord-345088-krb1eidw	286	70	30	30	CD
cord-345088-krb1eidw	286	71	min	min	NN
cord-345088-krb1eidw	286	72	.	.	.
cord-345088-krb1eidw	287	1	A	a	DT
cord-345088-krb1eidw	287	2	half	half	NN
cord-345088-krb1eidw	287	3	of	of	IN
cord-345088-krb1eidw	287	4	the	the	DT
cord-345088-krb1eidw	287	5	cells	cell	NNS
cord-345088-krb1eidw	287	6	were	be	VBD
cord-345088-krb1eidw	287	7	permeabilized	permeabilize	VBN
cord-345088-krb1eidw	287	8	with	with	IN
cord-345088-krb1eidw	287	9	0.1	0.1	CD
cord-345088-krb1eidw	287	10	%	%	NN
cord-345088-krb1eidw	287	11	saponin	saponin	NN
cord-345088-krb1eidw	287	12	in	in	IN
cord-345088-krb1eidw	287	13	FACS	FACS	NNP
cord-345088-krb1eidw	287	14	washing	washing	NN
cord-345088-krb1eidw	287	15	buffer	buffer	NN
cord-345088-krb1eidw	287	16	(	(	-LRB-
cord-345088-krb1eidw	287	17	2.5	2.5	CD
cord-345088-krb1eidw	287	18	%	%	NN
cord-345088-krb1eidw	287	19	FBS	FBS	NNP
cord-345088-krb1eidw	287	20	and	and	CC
cord-345088-krb1eidw	287	21	0.05	0.05	CD
cord-345088-krb1eidw	287	22	%	%	NN
cord-345088-krb1eidw	287	23	sodium	sodium	NN
cord-345088-krb1eidw	287	24	azide	azide	NN
cord-345088-krb1eidw	287	25	in	in	IN
cord-345088-krb1eidw	287	26	PBS	PBS	NNP
cord-345088-krb1eidw	287	27	)	)	-RRB-
cord-345088-krb1eidw	287	28	incubated	incubate	VBD
cord-345088-krb1eidw	287	29	for	for	IN
cord-345088-krb1eidw	287	30	10	10	CD
cord-345088-krb1eidw	287	31	min	min	NN
cord-345088-krb1eidw	287	32	at	at	IN
cord-345088-krb1eidw	287	33	room	room	NN
cord-345088-krb1eidw	287	34	temperature	temperature	NN
cord-345088-krb1eidw	287	35	,	,	,
cord-345088-krb1eidw	287	36	and	and	CC
cord-345088-krb1eidw	287	37	stained	stain	VBN
cord-345088-krb1eidw	287	38	with	with	IN
cord-345088-krb1eidw	287	39	1:100	1:100	CD
cord-345088-krb1eidw	287	40	diluted	dilute	VBN
cord-345088-krb1eidw	287	41	primary	primary	JJ
cord-345088-krb1eidw	287	42	antiserum	antiserum	NN
cord-345088-krb1eidw	287	43	,	,	,
cord-345088-krb1eidw	287	44	the	the	DT
cord-345088-krb1eidw	287	45	rabbit	rabbit	NN
cord-345088-krb1eidw	287	46	anti	anti	NNP
cord-345088-krb1eidw	287	47	-	-	NNP
cord-345088-krb1eidw	287	48	IBV	IBV	NNP
cord-345088-krb1eidw	287	49	S	S	NNP
cord-345088-krb1eidw	287	50	protein	protein	NN
cord-345088-krb1eidw	287	51	.	.	.
cord-345088-krb1eidw	288	1	The	the	DT
cord-345088-krb1eidw	288	2	other	other	JJ
cord-345088-krb1eidw	288	3	half	half	NN
cord-345088-krb1eidw	288	4	was	be	VBD
cord-345088-krb1eidw	288	5	stained	stain	VBN
cord-345088-krb1eidw	288	6	directly	directly	RB
cord-345088-krb1eidw	288	7	with	with	IN
cord-345088-krb1eidw	288	8	the	the	DT
cord-345088-krb1eidw	288	9	same	same	JJ
cord-345088-krb1eidw	288	10	primary	primary	JJ
cord-345088-krb1eidw	288	11	antibody	antibody	NN
cord-345088-krb1eidw	288	12	.	.	.
cord-345088-krb1eidw	289	1	Cells	cell	NNS
cord-345088-krb1eidw	289	2	were	be	VBD
cord-345088-krb1eidw	289	3	then	then	RB
cord-345088-krb1eidw	289	4	washed	wash	VBN
cord-345088-krb1eidw	289	5	two	two	CD
cord-345088-krb1eidw	289	6	times	time	NNS
cord-345088-krb1eidw	289	7	with	with	IN
cord-345088-krb1eidw	289	8	the	the	DT
cord-345088-krb1eidw	289	9	FACS	FACS	NNP
cord-345088-krb1eidw	289	10	washing	washing	NN
cord-345088-krb1eidw	289	11	buffer	buffer	NN
cord-345088-krb1eidw	289	12	,	,	,
cord-345088-krb1eidw	289	13	stained	stain	VBN
cord-345088-krb1eidw	289	14	with	with	IN
cord-345088-krb1eidw	289	15	1:20	1:20	CD
cord-345088-krb1eidw	289	16	diluted	diluted	JJ
cord-345088-krb1eidw	289	17	FITC	FITC	NNP
cord-345088-krb1eidw	289	18	conjugated	conjugate	VBD
cord-345088-krb1eidw	289	19	swine	swine	NN
cord-345088-krb1eidw	289	20	anti	anti	JJ
cord-345088-krb1eidw	289	21	-	-	JJ
cord-345088-krb1eidw	289	22	rabbit	rabbit	JJ
cord-345088-krb1eidw	289	23	antibody	antibody	NN
cord-345088-krb1eidw	289	24	(	(	-LRB-
cord-345088-krb1eidw	289	25	DAKO	DAKO	NNP
cord-345088-krb1eidw	289	26	)	)	-RRB-
cord-345088-krb1eidw	289	27	.	.	.
cord-345088-krb1eidw	290	1	After	after	IN
cord-345088-krb1eidw	290	2	washing	wash	VBG
cord-345088-krb1eidw	290	3	two	two	CD
cord-345088-krb1eidw	290	4	times	time	NNS
cord-345088-krb1eidw	290	5	with	with	IN
cord-345088-krb1eidw	290	6	the	the	DT
cord-345088-krb1eidw	290	7	FACS	FACS	NNP
cord-345088-krb1eidw	290	8	washing	washing	NN
cord-345088-krb1eidw	290	9	buffer	buffer	NN
cord-345088-krb1eidw	290	10	,	,	,
cord-345088-krb1eidw	290	11	cells	cell	NNS
cord-345088-krb1eidw	290	12	were	be	VBD
cord-345088-krb1eidw	290	13	then	then	RB
cord-345088-krb1eidw	290	14	fixed	fix	VBN
cord-345088-krb1eidw	290	15	with	with	IN
cord-345088-krb1eidw	290	16	1	1	CD
cord-345088-krb1eidw	290	17	%	%	NN
cord-345088-krb1eidw	290	18	ice	ice	NN
cord-345088-krb1eidw	290	19	cold	cold	JJ
cord-345088-krb1eidw	290	20	paraformaldehyde	paraformaldehyde	NNP
cord-345088-krb1eidw	290	21	and	and	CC
cord-345088-krb1eidw	290	22	analyzed	analyze	VBN
cord-345088-krb1eidw	290	23	by	by	IN
cord-345088-krb1eidw	290	24	flow	flow	NN
cord-345088-krb1eidw	290	25	cytometry	cytometry	NN
cord-345088-krb1eidw	290	26	.	.	.
cord-345088-krb1eidw	291	1	Two	two	CD
cord-345088-krb1eidw	291	2	amino	amino	NN
cord-345088-krb1eidw	291	3	acid	acid	NN
cord-345088-krb1eidw	291	4	changes	change	NNS
cord-345088-krb1eidw	291	5	at	at	IN
cord-345088-krb1eidw	291	6	the	the	DT
cord-345088-krb1eidw	291	7	N	n	CD
cord-345088-krb1eidw	291	8	-	-	HYPH
cord-345088-krb1eidw	291	9	terminus	terminus	NN
cord-345088-krb1eidw	291	10	of	of	IN
cord-345088-krb1eidw	291	11	transmissible	transmissible	JJ
cord-345088-krb1eidw	291	12	gastroenteritis	gastroenteritis	JJ
cord-345088-krb1eidw	291	13	coronavirus	coronavirus	NN
cord-345088-krb1eidw	291	14	spike	spike	NN
cord-345088-krb1eidw	291	15	protein	protein	NN
cord-345088-krb1eidw	291	16	result	result	NN
cord-345088-krb1eidw	291	17	in	in	IN
cord-345088-krb1eidw	291	18	the	the	DT
cord-345088-krb1eidw	291	19	loss	loss	NN
cord-345088-krb1eidw	291	20	of	of	IN
cord-345088-krb1eidw	291	21	enteric	enteric	JJ
cord-345088-krb1eidw	291	22	tropism	tropism	NN
cord-345088-krb1eidw	291	23	Coronavirus	Coronavirus	NNP
cord-345088-krb1eidw	291	24	-	-	HYPH
cord-345088-krb1eidw	291	25	induced	induce	VBN
cord-345088-krb1eidw	291	26	membrane	membrane	NN
cord-345088-krb1eidw	291	27	fusion	fusion	NN
cord-345088-krb1eidw	292	1	requires	require	VBZ
cord-345088-krb1eidw	292	2	the	the	DT
cord-345088-krb1eidw	292	3	cysteine	cysteine	NN
cord-345088-krb1eidw	292	4	-	-	HYPH
cord-345088-krb1eidw	292	5	rich	rich	JJ
cord-345088-krb1eidw	292	6	domain	domain	NN
cord-345088-krb1eidw	292	7	in	in	IN
cord-345088-krb1eidw	292	8	the	the	DT
cord-345088-krb1eidw	292	9	spike	spike	NN
cord-345088-krb1eidw	292	10	protein	protein	NN
cord-345088-krb1eidw	292	11	Evidence	evidence	NN
cord-345088-krb1eidw	292	12	for	for	IN
cord-345088-krb1eidw	292	13	a	a	DT
cord-345088-krb1eidw	292	14	coiled	coil	VBN
cord-345088-krb1eidw	292	15	-	-	HYPH
cord-345088-krb1eidw	292	16	coil	coil	NN
cord-345088-krb1eidw	292	17	structure	structure	NN
cord-345088-krb1eidw	292	18	in	in	IN
cord-345088-krb1eidw	292	19	the	the	DT
cord-345088-krb1eidw	292	20	spike	spike	NN
cord-345088-krb1eidw	292	21	of	of	IN
cord-345088-krb1eidw	292	22	coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	292	23	Mapping	mapping	NN
cord-345088-krb1eidw	292	24	of	of	IN
cord-345088-krb1eidw	292	25	the	the	DT
cord-345088-krb1eidw	292	26	coronavirus	coronavirus	NN
cord-345088-krb1eidw	292	27	membrane	membrane	NN
cord-345088-krb1eidw	292	28	protein	protein	NN
cord-345088-krb1eidw	292	29	domains	domain	NNS
cord-345088-krb1eidw	292	30	involved	involve	VBN
cord-345088-krb1eidw	292	31	in	in	IN
cord-345088-krb1eidw	292	32	interaction	interaction	NN
cord-345088-krb1eidw	292	33	with	with	IN
cord-345088-krb1eidw	292	34	the	the	DT
cord-345088-krb1eidw	292	35	spike	spike	NN
cord-345088-krb1eidw	292	36	protein	protein	NN
cord-345088-krb1eidw	292	37	Identification	identification	NN
cord-345088-krb1eidw	292	38	of	of	IN
cord-345088-krb1eidw	292	39	a	a	DT
cord-345088-krb1eidw	292	40	novel	novel	JJ
cord-345088-krb1eidw	292	41	coronavirus	coronavirus	NN
cord-345088-krb1eidw	292	42	in	in	IN
cord-345088-krb1eidw	292	43	patients	patient	NNS
cord-345088-krb1eidw	292	44	with	with	IN
cord-345088-krb1eidw	292	45	severe	severe	JJ
cord-345088-krb1eidw	292	46	acute	acute	JJ
cord-345088-krb1eidw	292	47	respiratory	respiratory	JJ
cord-345088-krb1eidw	292	48	syndrome	syndrome	NN
cord-345088-krb1eidw	292	49	Aetiology	aetiology	NN
cord-345088-krb1eidw	292	50	:	:	:
cord-345088-krb1eidw	293	1	Koch	Koch	NNP
cord-345088-krb1eidw	293	2	's	's	POS
cord-345088-krb1eidw	293	3	postulates	postulate	NNS
cord-345088-krb1eidw	293	4	fulfilled	fulfil	VBD
cord-345088-krb1eidw	293	5	for	for	IN
cord-345088-krb1eidw	293	6	SARS	SARS	NNP
cord-345088-krb1eidw	293	7	virus	virus	NN
cord-345088-krb1eidw	293	8	Proteolytic	proteolytic	JJ
cord-345088-krb1eidw	293	9	cleavage	cleavage	NN
cord-345088-krb1eidw	293	10	of	of	IN
cord-345088-krb1eidw	293	11	the	the	DT
cord-345088-krb1eidw	293	12	E2	E2	NNP
cord-345088-krb1eidw	293	13	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	293	14	of	of	IN
cord-345088-krb1eidw	293	15	murine	murine	JJ
cord-345088-krb1eidw	293	16	coronavirus	coronavirus	NN
cord-345088-krb1eidw	293	17	:	:	:
cord-345088-krb1eidw	293	18	host	host	NN
cord-345088-krb1eidw	293	19	-	-	HYPH
cord-345088-krb1eidw	293	20	dependent	dependent	JJ
cord-345088-krb1eidw	293	21	differences	difference	NNS
cord-345088-krb1eidw	293	22	in	in	IN
cord-345088-krb1eidw	293	23	proteolytic	proteolytic	JJ
cord-345088-krb1eidw	293	24	cleavage	cleavage	NN
cord-345088-krb1eidw	293	25	and	and	CC
cord-345088-krb1eidw	293	26	cell	cell	NN
cord-345088-krb1eidw	293	27	fusion	fusion	NN
cord-345088-krb1eidw	293	28	Alteration	alteration	NN
cord-345088-krb1eidw	293	29	of	of	IN
cord-345088-krb1eidw	293	30	the	the	DT
cord-345088-krb1eidw	293	31	pH	pH	NNP
cord-345088-krb1eidw	293	32	dependence	dependence	NN
cord-345088-krb1eidw	293	33	of	of	IN
cord-345088-krb1eidw	293	34	coronavirus	coronavirus	NN
cord-345088-krb1eidw	293	35	-	-	HYPH
cord-345088-krb1eidw	293	36	induced	induce	VBN
cord-345088-krb1eidw	293	37	cell	cell	NN
cord-345088-krb1eidw	293	38	fusion	fusion	NN
cord-345088-krb1eidw	293	39	:	:	:
cord-345088-krb1eidw	293	40	effect	effect	NN
cord-345088-krb1eidw	293	41	of	of	IN
cord-345088-krb1eidw	293	42	mutations	mutation	NNS
cord-345088-krb1eidw	293	43	in	in	IN
cord-345088-krb1eidw	293	44	the	the	DT
cord-345088-krb1eidw	293	45	spike	spike	NN
cord-345088-krb1eidw	293	46	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	293	47	Cell	cell	NN
cord-345088-krb1eidw	293	48	receptor	receptor	NN
cord-345088-krb1eidw	293	49	-	-	HYPH
cord-345088-krb1eidw	293	50	independent	independent	JJ
cord-345088-krb1eidw	293	51	infection	infection	NN
cord-345088-krb1eidw	293	52	by	by	IN
cord-345088-krb1eidw	293	53	a	a	DT
cord-345088-krb1eidw	293	54	neurotropic	neurotropic	NNP
cord-345088-krb1eidw	293	55	murine	murine	JJ
cord-345088-krb1eidw	293	56	coronavirus	coronavirus	NN
cord-345088-krb1eidw	293	57	Major	major	JJ
cord-345088-krb1eidw	293	58	receptorbinding	receptorbinding	NN
cord-345088-krb1eidw	293	59	and	and	CC
cord-345088-krb1eidw	293	60	neutralization	neutralization	NN
cord-345088-krb1eidw	293	61	determinants	determinant	NNS
cord-345088-krb1eidw	293	62	are	be	VBP
cord-345088-krb1eidw	293	63	located	locate	VBN
cord-345088-krb1eidw	293	64	within	within	IN
cord-345088-krb1eidw	293	65	the	the	DT
cord-345088-krb1eidw	293	66	same	same	JJ
cord-345088-krb1eidw	293	67	domain	domain	NN
cord-345088-krb1eidw	293	68	of	of	IN
cord-345088-krb1eidw	293	69	the	the	DT
cord-345088-krb1eidw	293	70	transmissible	transmissible	JJ
cord-345088-krb1eidw	293	71	gastroenteritis	gastroenteritis	NN
cord-345088-krb1eidw	293	72	virus	virus	NN
cord-345088-krb1eidw	293	73	(	(	-LRB-
cord-345088-krb1eidw	293	74	coronavirus	coronavirus	NN
cord-345088-krb1eidw	293	75	)	)	-RRB-
cord-345088-krb1eidw	293	76	spike	spike	NN
cord-345088-krb1eidw	293	77	protein	protein	NN
cord-345088-krb1eidw	293	78	Folding	folding	NN
cord-345088-krb1eidw	293	79	of	of	IN
cord-345088-krb1eidw	293	80	VSV	VSV	NNP
cord-345088-krb1eidw	293	81	G	G	NNP
cord-345088-krb1eidw	293	82	protein	protein	NN
cord-345088-krb1eidw	293	83	:	:	:
cord-345088-krb1eidw	293	84	sequential	sequential	JJ
cord-345088-krb1eidw	293	85	interaction	interaction	NN
cord-345088-krb1eidw	293	86	with	with	IN
cord-345088-krb1eidw	293	87	BiP	BiP	NNP
cord-345088-krb1eidw	293	88	and	and	CC
cord-345088-krb1eidw	293	89	calnexin	calnexin	NNP
cord-345088-krb1eidw	293	90	Calnexin	Calnexin	NNP
cord-345088-krb1eidw	293	91	and	and	CC
cord-345088-krb1eidw	293	92	calreticulin	calreticulin	NNP
cord-345088-krb1eidw	293	93	promote	promote	VBP
cord-345088-krb1eidw	293	94	folding	folding	NN
cord-345088-krb1eidw	293	95	,	,	,
cord-345088-krb1eidw	293	96	delay	delay	VB
cord-345088-krb1eidw	293	97	oligomerization	oligomerization	NN
cord-345088-krb1eidw	293	98	and	and	CC
cord-345088-krb1eidw	293	99	suppress	suppress	VB
cord-345088-krb1eidw	293	100	degradation	degradation	NN
cord-345088-krb1eidw	293	101	of	of	IN
cord-345088-krb1eidw	293	102	influenza	influenza	NN
cord-345088-krb1eidw	293	103	hemagglutinin	hemagglutinin	NN
cord-345088-krb1eidw	293	104	in	in	IN
cord-345088-krb1eidw	293	105	microsomes	microsome	NNS
cord-345088-krb1eidw	294	1	Tunicamycin	Tunicamycin	NNP
cord-345088-krb1eidw	294	2	resistant	resistant	JJ
cord-345088-krb1eidw	294	3	glycosylation	glycosylation	NN
cord-345088-krb1eidw	294	4	of	of	IN
cord-345088-krb1eidw	294	5	coronavirus	coronavirus	NN
cord-345088-krb1eidw	294	6	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	294	7	:	:	:
cord-345088-krb1eidw	294	8	demonstration	demonstration	NN
cord-345088-krb1eidw	294	9	of	of	IN
cord-345088-krb1eidw	294	10	a	a	DT
cord-345088-krb1eidw	294	11	novel	novel	JJ
cord-345088-krb1eidw	294	12	type	type	NN
cord-345088-krb1eidw	294	13	of	of	IN
cord-345088-krb1eidw	294	14	viral	viral	JJ
cord-345088-krb1eidw	294	15	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	294	16	Characterization	characterization	NN
cord-345088-krb1eidw	294	17	of	of	IN
cord-345088-krb1eidw	294	18	the	the	DT
cord-345088-krb1eidw	294	19	budding	bud	VBG
cord-345088-krb1eidw	294	20	compartment	compartment	NN
cord-345088-krb1eidw	294	21	of	of	IN
cord-345088-krb1eidw	294	22	mouse	mouse	NNP
cord-345088-krb1eidw	294	23	hepatitis	hepatitis	NNP
cord-345088-krb1eidw	294	24	virus	virus	NN
cord-345088-krb1eidw	294	25	:	:	:
cord-345088-krb1eidw	294	26	evidence	evidence	NN
cord-345088-krb1eidw	294	27	that	that	DT
cord-345088-krb1eidw	294	28	transport	transport	NN
cord-345088-krb1eidw	294	29	from	from	IN
cord-345088-krb1eidw	294	30	the	the	DT
cord-345088-krb1eidw	294	31	RER	RER	NNP
cord-345088-krb1eidw	294	32	to	to	IN
cord-345088-krb1eidw	294	33	the	the	DT
cord-345088-krb1eidw	294	34	Golgi	Golgi	NNP
cord-345088-krb1eidw	294	35	complex	complex	NN
cord-345088-krb1eidw	294	36	requires	require	VBZ
cord-345088-krb1eidw	294	37	only	only	RB
cord-345088-krb1eidw	294	38	one	one	CD
cord-345088-krb1eidw	294	39	vesicular	vesicular	JJ
cord-345088-krb1eidw	294	40	transport	transport	NN
cord-345088-krb1eidw	294	41	step	step	NN
cord-345088-krb1eidw	294	42	Variations	variation	NNS
cord-345088-krb1eidw	294	43	in	in	IN
cord-345088-krb1eidw	294	44	disparate	disparate	JJ
cord-345088-krb1eidw	294	45	regions	region	NNS
cord-345088-krb1eidw	294	46	of	of	IN
cord-345088-krb1eidw	294	47	the	the	DT
cord-345088-krb1eidw	294	48	murine	murine	JJ
cord-345088-krb1eidw	294	49	coronavirus	coronavirus	NN
cord-345088-krb1eidw	294	50	spike	spike	NN
cord-345088-krb1eidw	294	51	protein	protein	NN
cord-345088-krb1eidw	294	52	impact	impact	VBP
cord-345088-krb1eidw	294	53	the	the	DT
cord-345088-krb1eidw	294	54	initiation	initiation	NN
cord-345088-krb1eidw	294	55	of	of	IN
cord-345088-krb1eidw	294	56	membrane	membrane	NN
cord-345088-krb1eidw	294	57	fusion	fusion	NN
cord-345088-krb1eidw	294	58	Localization	localization	NN
cord-345088-krb1eidw	294	59	of	of	IN
cord-345088-krb1eidw	294	60	neutralizing	neutralize	VBG
cord-345088-krb1eidw	294	61	epitopes	epitope	NNS
cord-345088-krb1eidw	294	62	and	and	CC
cord-345088-krb1eidw	294	63	the	the	DT
cord-345088-krb1eidw	294	64	receptor	receptor	NN
cord-345088-krb1eidw	294	65	-	-	HYPH
cord-345088-krb1eidw	294	66	binding	bind	VBG
cord-345088-krb1eidw	294	67	site	site	NN
cord-345088-krb1eidw	294	68	within	within	IN
cord-345088-krb1eidw	294	69	the	the	DT
cord-345088-krb1eidw	294	70	amino	amino	NN
cord-345088-krb1eidw	294	71	-	-	HYPH
cord-345088-krb1eidw	294	72	terminal	terminal	NN
cord-345088-krb1eidw	294	73	330	330	CD
cord-345088-krb1eidw	294	74	amino	amino	NN
cord-345088-krb1eidw	294	75	acids	acid	NNS
cord-345088-krb1eidw	294	76	of	of	IN
cord-345088-krb1eidw	294	77	the	the	DT
cord-345088-krb1eidw	294	78	murine	murine	JJ
cord-345088-krb1eidw	294	79	coronavirus	coronavirus	NN
cord-345088-krb1eidw	294	80	spike	spike	NN
cord-345088-krb1eidw	294	81	protein	protein	NN
cord-345088-krb1eidw	294	82	Retargeting	retargeting	NN
cord-345088-krb1eidw	294	83	of	of	IN
cord-345088-krb1eidw	294	84	coronavirus	coronavirus	NN
cord-345088-krb1eidw	294	85	by	by	IN
cord-345088-krb1eidw	294	86	substitution	substitution	NN
cord-345088-krb1eidw	294	87	of	of	IN
cord-345088-krb1eidw	294	88	the	the	DT
cord-345088-krb1eidw	294	89	spike	spike	NN
cord-345088-krb1eidw	294	90	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	294	91	ectodomain	ectodomain	NNP
cord-345088-krb1eidw	294	92	:	:	:
cord-345088-krb1eidw	294	93	crossing	cross	VBG
cord-345088-krb1eidw	294	94	the	the	DT
cord-345088-krb1eidw	294	95	host	host	NN
cord-345088-krb1eidw	294	96	cell	cell	NN
cord-345088-krb1eidw	294	97	species	specie	NNS
cord-345088-krb1eidw	294	98	barrier	barrier	NN
cord-345088-krb1eidw	295	1	The	the	DT
cord-345088-krb1eidw	295	2	molecular	molecular	JJ
cord-345088-krb1eidw	295	3	biology	biology	NN
cord-345088-krb1eidw	295	4	of	of	IN
cord-345088-krb1eidw	295	5	coronaviruses	coronaviruse	NNS
cord-345088-krb1eidw	295	6	Altered	alter	VBN
cord-345088-krb1eidw	296	1	pathogenesis	pathogenesis	NN
cord-345088-krb1eidw	296	2	of	of	IN
cord-345088-krb1eidw	296	3	a	a	DT
cord-345088-krb1eidw	296	4	mutant	mutant	NN
cord-345088-krb1eidw	296	5	of	of	IN
cord-345088-krb1eidw	296	6	the	the	DT
cord-345088-krb1eidw	296	7	murine	murine	JJ
cord-345088-krb1eidw	296	8	coronavirus	coronavirus	NN
cord-345088-krb1eidw	296	9	MHV	MHV	NNP
cord-345088-krb1eidw	296	10	-	-	HYPH
cord-345088-krb1eidw	296	11	A59	A59	NNP
cord-345088-krb1eidw	296	12	is	be	VBZ
cord-345088-krb1eidw	296	13	associated	associate	VBN
cord-345088-krb1eidw	296	14	with	with	IN
cord-345088-krb1eidw	296	15	a	a	DT
cord-345088-krb1eidw	296	16	Q159L	Q159L	NNP
cord-345088-krb1eidw	296	17	amino	amino	NN
cord-345088-krb1eidw	296	18	acid	acid	NN
cord-345088-krb1eidw	296	19	substitution	substitution	NN
cord-345088-krb1eidw	296	20	in	in	IN
cord-345088-krb1eidw	296	21	the	the	DT
cord-345088-krb1eidw	296	22	spike	spike	NN
cord-345088-krb1eidw	296	23	protein	protein	NN
cord-345088-krb1eidw	296	24	Association	Association	NNP
cord-345088-krb1eidw	296	25	of	of	IN
cord-345088-krb1eidw	296	26	the	the	DT
cord-345088-krb1eidw	296	27	infectious	infectious	JJ
cord-345088-krb1eidw	296	28	bronchitis	bronchitis	NN
cord-345088-krb1eidw	296	29	virus	virus	NN
cord-345088-krb1eidw	296	30	3c	3c	NNP
cord-345088-krb1eidw	296	31	protein	protein	NN
cord-345088-krb1eidw	296	32	with	with	IN
cord-345088-krb1eidw	296	33	the	the	DT
cord-345088-krb1eidw	296	34	virion	virion	NN
cord-345088-krb1eidw	296	35	envelope	envelope	NN
cord-345088-krb1eidw	296	36	Proteolytic	proteolytic	JJ
cord-345088-krb1eidw	296	37	mapping	mapping	NN
cord-345088-krb1eidw	296	38	of	of	IN
cord-345088-krb1eidw	296	39	the	the	DT
cord-345088-krb1eidw	296	40	coronavirus	coronavirus	NN
cord-345088-krb1eidw	296	41	infectious	infectious	JJ
cord-345088-krb1eidw	296	42	virus	virus	NN
cord-345088-krb1eidw	296	43	1b	1b	CD
cord-345088-krb1eidw	296	44	polyprotein	polyprotein	NNP
cord-345088-krb1eidw	296	45	:	:	:
cord-345088-krb1eidw	296	46	evidence	evidence	NN
cord-345088-krb1eidw	296	47	for	for	IN
cord-345088-krb1eidw	296	48	the	the	DT
cord-345088-krb1eidw	296	49	presence	presence	NN
cord-345088-krb1eidw	296	50	of	of	IN
cord-345088-krb1eidw	296	51	four	four	CD
cord-345088-krb1eidw	296	52	cleavage	cleavage	NN
cord-345088-krb1eidw	296	53	sites	site	NNS
cord-345088-krb1eidw	296	54	of	of	IN
cord-345088-krb1eidw	296	55	the	the	DT
cord-345088-krb1eidw	296	56	3C	3c	NN
cord-345088-krb1eidw	296	57	-	-	HYPH
cord-345088-krb1eidw	296	58	like	like	JJ
cord-345088-krb1eidw	296	59	proteinase	proteinase	NN
cord-345088-krb1eidw	296	60	and	and	CC
cord-345088-krb1eidw	296	61	identification	identification	NN
cord-345088-krb1eidw	296	62	of	of	IN
cord-345088-krb1eidw	296	63	two	two	CD
cord-345088-krb1eidw	296	64	novel	novel	JJ
cord-345088-krb1eidw	296	65	cleavage	cleavage	NN
cord-345088-krb1eidw	296	66	products	product	NNS
cord-345088-krb1eidw	296	67	Roles	role	NNS
cord-345088-krb1eidw	296	68	in	in	IN
cord-345088-krb1eidw	296	69	cell	cell	NN
cord-345088-krb1eidw	296	70	-	-	HYPH
cord-345088-krb1eidw	296	71	to	to	IN
cord-345088-krb1eidw	296	72	-	-	HYPH
cord-345088-krb1eidw	296	73	cell	cell	NN
cord-345088-krb1eidw	296	74	fusion	fusion	NN
cord-345088-krb1eidw	296	75	of	of	IN
cord-345088-krb1eidw	296	76	two	two	CD
cord-345088-krb1eidw	296	77	conserved	conserved	JJ
cord-345088-krb1eidw	296	78	hydrophobic	hydrophobic	JJ
cord-345088-krb1eidw	296	79	regions	region	NNS
cord-345088-krb1eidw	296	80	in	in	IN
cord-345088-krb1eidw	296	81	the	the	DT
cord-345088-krb1eidw	296	82	murine	murine	JJ
cord-345088-krb1eidw	296	83	coronavirus	coronavirus	NN
cord-345088-krb1eidw	296	84	spike	spike	NN
cord-345088-krb1eidw	296	85	protein	protein	NN
cord-345088-krb1eidw	296	86	Amino	amino	NN
cord-345088-krb1eidw	296	87	acid	acid	NN
cord-345088-krb1eidw	296	88	substitutions	substitution	NNS
cord-345088-krb1eidw	296	89	within	within	IN
cord-345088-krb1eidw	296	90	the	the	DT
cord-345088-krb1eidw	296	91	leucine	leucine	NN
cord-345088-krb1eidw	296	92	zipper	zipper	NN
cord-345088-krb1eidw	296	93	domain	domain	NN
cord-345088-krb1eidw	296	94	of	of	IN
cord-345088-krb1eidw	296	95	the	the	DT
cord-345088-krb1eidw	296	96	murine	murine	JJ
cord-345088-krb1eidw	296	97	coronavirus	coronavirus	NN
cord-345088-krb1eidw	296	98	spike	spike	NN
cord-345088-krb1eidw	296	99	protein	protein	NN
cord-345088-krb1eidw	296	100	cause	cause	VBP
cord-345088-krb1eidw	296	101	defects	defect	NNS
cord-345088-krb1eidw	296	102	in	in	IN
cord-345088-krb1eidw	296	103	oligomerization	oligomerization	NN
cord-345088-krb1eidw	296	104	and	and	CC
cord-345088-krb1eidw	296	105	the	the	DT
cord-345088-krb1eidw	296	106	ability	ability	NN
cord-345088-krb1eidw	296	107	to	to	TO
cord-345088-krb1eidw	296	108	induce	induce	VB
cord-345088-krb1eidw	296	109	cell	cell	NN
cord-345088-krb1eidw	296	110	-	-	HYPH
cord-345088-krb1eidw	296	111	tocell	tocell	NN
cord-345088-krb1eidw	296	112	fusion	fusion	NN
cord-345088-krb1eidw	296	113	Characterization	characterization	NN
cord-345088-krb1eidw	296	114	of	of	IN
cord-345088-krb1eidw	296	115	two	two	CD
cord-345088-krb1eidw	296	116	temperature	temperature	NN
cord-345088-krb1eidw	296	117	-	-	HYPH
cord-345088-krb1eidw	296	118	sensitive	sensitive	JJ
cord-345088-krb1eidw	296	119	mutants	mutant	NNS
cord-345088-krb1eidw	296	120	of	of	IN
cord-345088-krb1eidw	296	121	coronavirus	coronavirus	NN
cord-345088-krb1eidw	296	122	mouse	mouse	NN
cord-345088-krb1eidw	296	123	hepatitis	hepatitis	NN
cord-345088-krb1eidw	296	124	virus	virus	NN
cord-345088-krb1eidw	296	125	strain	strain	VBP
cord-345088-krb1eidw	296	126	A59	A59	NNP
cord-345088-krb1eidw	296	127	with	with	IN
cord-345088-krb1eidw	296	128	maturation	maturation	NN
cord-345088-krb1eidw	296	129	defects	defect	NNS
cord-345088-krb1eidw	296	130	in	in	IN
cord-345088-krb1eidw	296	131	the	the	DT
cord-345088-krb1eidw	296	132	spike	spike	NN
cord-345088-krb1eidw	296	133	protein	protein	NN
cord-345088-krb1eidw	296	134	Spike	Spike	NNP
cord-345088-krb1eidw	296	135	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	296	136	-	-	HYPH
cord-345088-krb1eidw	296	137	mediated	mediate	VBN
cord-345088-krb1eidw	296	138	fusion	fusion	NN
cord-345088-krb1eidw	296	139	in	in	IN
cord-345088-krb1eidw	296	140	biliary	biliary	JJ
cord-345088-krb1eidw	296	141	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	296	142	-	-	HYPH
cord-345088-krb1eidw	296	143	independent	independent	JJ
cord-345088-krb1eidw	296	144	cell	cell	NN
cord-345088-krb1eidw	296	145	-	-	HYPH
cord-345088-krb1eidw	296	146	associated	associate	VBN
cord-345088-krb1eidw	296	147	spread	spread	NN
cord-345088-krb1eidw	296	148	of	of	IN
cord-345088-krb1eidw	296	149	mouse	mouse	NN
cord-345088-krb1eidw	296	150	hepatitis	hepatitis	NNP
cord-345088-krb1eidw	296	151	virus	virus	NN
cord-345088-krb1eidw	296	152	infection	infection	NN
cord-345088-krb1eidw	297	1	Murine	murine	JJ
cord-345088-krb1eidw	297	2	coronavirus	coronavirus	NN
cord-345088-krb1eidw	297	3	spike	spike	NN
cord-345088-krb1eidw	297	4	protein	protein	NN
cord-345088-krb1eidw	297	5	determines	determine	VBZ
cord-345088-krb1eidw	297	6	the	the	DT
cord-345088-krb1eidw	297	7	ability	ability	NN
cord-345088-krb1eidw	297	8	of	of	IN
cord-345088-krb1eidw	297	9	the	the	DT
cord-345088-krb1eidw	297	10	virus	virus	NN
cord-345088-krb1eidw	297	11	to	to	TO
cord-345088-krb1eidw	297	12	replicate	replicate	VB
cord-345088-krb1eidw	297	13	in	in	IN
cord-345088-krb1eidw	297	14	the	the	DT
cord-345088-krb1eidw	297	15	liver	liver	NN
cord-345088-krb1eidw	297	16	and	and	CC
cord-345088-krb1eidw	297	17	cause	cause	VB
cord-345088-krb1eidw	297	18	hepatitis	hepatitis	NN
cord-345088-krb1eidw	297	19	Disulfide	Disulfide	NNP
cord-345088-krb1eidw	297	20	bonds	bond	NNS
cord-345088-krb1eidw	297	21	in	in	IN
cord-345088-krb1eidw	297	22	folding	folding	NN
cord-345088-krb1eidw	297	23	and	and	CC
cord-345088-krb1eidw	297	24	transport	transport	NN
cord-345088-krb1eidw	297	25	of	of	IN
cord-345088-krb1eidw	297	26	mouse	mouse	NN
cord-345088-krb1eidw	297	27	hepatitis	hepatitis	NN
cord-345088-krb1eidw	297	28	coronavirus	coronavirus	NN
cord-345088-krb1eidw	297	29	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	297	30	Envelope	Envelope	NNP
cord-345088-krb1eidw	297	31	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	297	32	interactions	interaction	NNS
cord-345088-krb1eidw	297	33	in	in	IN
cord-345088-krb1eidw	297	34	coronavirus	coronavirus	NN
cord-345088-krb1eidw	297	35	assembly	assembly	NN
cord-345088-krb1eidw	298	1	A	a	DT
cord-345088-krb1eidw	298	2	conditional	conditional	JJ
cord-345088-krb1eidw	298	3	-	-	HYPH
cord-345088-krb1eidw	298	4	lethal	lethal	JJ
cord-345088-krb1eidw	298	5	murine	murine	JJ
cord-345088-krb1eidw	298	6	coronavirus	coronavirus	NN
cord-345088-krb1eidw	298	7	mutant	mutant	NN
cord-345088-krb1eidw	298	8	that	that	WDT
cord-345088-krb1eidw	298	9	fails	fail	VBZ
cord-345088-krb1eidw	298	10	to	to	TO
cord-345088-krb1eidw	298	11	incorporate	incorporate	VB
cord-345088-krb1eidw	298	12	the	the	DT
cord-345088-krb1eidw	298	13	spike	spike	NN
cord-345088-krb1eidw	298	14	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	298	15	into	into	IN
cord-345088-krb1eidw	298	16	assembled	assemble	VBN
cord-345088-krb1eidw	298	17	virions	virion	NNS
cord-345088-krb1eidw	298	18	Evolution	Evolution	NNP
cord-345088-krb1eidw	298	19	of	of	IN
cord-345088-krb1eidw	298	20	mouse	mouse	NN
cord-345088-krb1eidw	298	21	hepatitis	hepatitis	NNP
cord-345088-krb1eidw	298	22	virus	virus	NN
cord-345088-krb1eidw	298	23	:	:	:
cord-345088-krb1eidw	298	24	detection	detection	NN
cord-345088-krb1eidw	298	25	and	and	CC
cord-345088-krb1eidw	298	26	characterization	characterization	NN
cord-345088-krb1eidw	298	27	of	of	IN
cord-345088-krb1eidw	298	28	spike	spike	NN
cord-345088-krb1eidw	298	29	deletion	deletion	NN
cord-345088-krb1eidw	298	30	variants	variant	NNS
cord-345088-krb1eidw	298	31	during	during	IN
cord-345088-krb1eidw	298	32	persistent	persistent	JJ
cord-345088-krb1eidw	298	33	infection	infection	NN
cord-345088-krb1eidw	298	34	Generation	generation	NN
cord-345088-krb1eidw	298	35	of	of	IN
cord-345088-krb1eidw	298	36	coronavirus	coronavirus	NN
cord-345088-krb1eidw	298	37	spike	spike	NN
cord-345088-krb1eidw	298	38	deletion	deletion	NN
cord-345088-krb1eidw	298	39	variants	variant	NNS
cord-345088-krb1eidw	298	40	by	by	IN
cord-345088-krb1eidw	298	41	high	high	JJ
cord-345088-krb1eidw	298	42	-	-	HYPH
cord-345088-krb1eidw	298	43	frequency	frequency	NN
cord-345088-krb1eidw	298	44	recombination	recombination	NN
cord-345088-krb1eidw	298	45	at	at	IN
cord-345088-krb1eidw	298	46	regions	region	NNS
cord-345088-krb1eidw	298	47	of	of	IN
cord-345088-krb1eidw	298	48	predicted	predict	VBN
cord-345088-krb1eidw	298	49	RNA	RNA	NNP
cord-345088-krb1eidw	298	50	secondary	secondary	JJ
cord-345088-krb1eidw	298	51	structure	structure	NN
cord-345088-krb1eidw	298	52	Identification	identification	NN
cord-345088-krb1eidw	298	53	of	of	IN
cord-345088-krb1eidw	298	54	spike	spike	NN
cord-345088-krb1eidw	298	55	protein	protein	NN
cord-345088-krb1eidw	298	56	residues	residue	NNS
cord-345088-krb1eidw	298	57	of	of	IN
cord-345088-krb1eidw	298	58	murine	murine	JJ
cord-345088-krb1eidw	298	59	coronavirus	coronavirus	NN
cord-345088-krb1eidw	298	60	responsible	responsible	JJ
cord-345088-krb1eidw	298	61	for	for	IN
cord-345088-krb1eidw	298	62	receptor	receptor	NN
cord-345088-krb1eidw	298	63	-	-	HYPH
cord-345088-krb1eidw	298	64	binding	bind	VBG
cord-345088-krb1eidw	298	65	activity	activity	NN
cord-345088-krb1eidw	298	66	by	by	IN
cord-345088-krb1eidw	298	67	use	use	NN
cord-345088-krb1eidw	298	68	of	of	IN
cord-345088-krb1eidw	298	69	soluble	soluble	JJ
cord-345088-krb1eidw	298	70	receptor	receptor	NN
cord-345088-krb1eidw	298	71	-	-	HYPH
cord-345088-krb1eidw	298	72	resistant	resistant	JJ
cord-345088-krb1eidw	298	73	mutants	mutant	NNS
cord-345088-krb1eidw	298	74	Determination	determination	NN
cord-345088-krb1eidw	298	75	of	of	IN
cord-345088-krb1eidw	298	76	the	the	DT
cord-345088-krb1eidw	298	77	complete	complete	JJ
cord-345088-krb1eidw	298	78	nucleotide	nucleotide	JJ
cord-345088-krb1eidw	298	79	sequence	sequence	NN
cord-345088-krb1eidw	298	80	of	of	IN
cord-345088-krb1eidw	298	81	a	a	DT
cord-345088-krb1eidw	298	82	vaccine	vaccine	NN
cord-345088-krb1eidw	298	83	strain	strain	NN
cord-345088-krb1eidw	298	84	of	of	IN
cord-345088-krb1eidw	298	85	porcine	porcine	JJ
cord-345088-krb1eidw	298	86	reproductive	reproductive	JJ
cord-345088-krb1eidw	298	87	and	and	CC
cord-345088-krb1eidw	298	88	respiratory	respiratory	JJ
cord-345088-krb1eidw	298	89	syndrome	syndrome	NN
cord-345088-krb1eidw	298	90	virus	virus	NN
cord-345088-krb1eidw	298	91	and	and	CC
cord-345088-krb1eidw	298	92	identification	identification	NN
cord-345088-krb1eidw	298	93	of	of	IN
cord-345088-krb1eidw	298	94	the	the	DT
cord-345088-krb1eidw	298	95	NSP2	NSP2	NNP
cord-345088-krb1eidw	298	96	gene	gene	NN
cord-345088-krb1eidw	298	97	with	with	IN
cord-345088-krb1eidw	298	98	an	an	DT
cord-345088-krb1eidw	298	99	unique	unique	JJ
cord-345088-krb1eidw	298	100	insertion	insertion	NN
cord-345088-krb1eidw	298	101	Emergence	emergence	NN
cord-345088-krb1eidw	298	102	of	of	IN
cord-345088-krb1eidw	298	103	a	a	DT
cord-345088-krb1eidw	298	104	coronavirus	coronavirus	NN
cord-345088-krb1eidw	298	105	infectious	infectious	JJ
cord-345088-krb1eidw	298	106	bronchitis	bronchitis	NN
cord-345088-krb1eidw	298	107	virus	virus	NN
cord-345088-krb1eidw	298	108	mutant	mutant	JJ
cord-345088-krb1eidw	298	109	with	with	IN
cord-345088-krb1eidw	298	110	a	a	DT
cord-345088-krb1eidw	298	111	truncated	truncated	JJ
cord-345088-krb1eidw	298	112	3b	3b	CD
cord-345088-krb1eidw	298	113	gene	gene	NN
cord-345088-krb1eidw	298	114	:	:	:
cord-345088-krb1eidw	298	115	functional	functional	JJ
cord-345088-krb1eidw	298	116	characterization	characterization	NN
cord-345088-krb1eidw	298	117	of	of	IN
cord-345088-krb1eidw	298	118	the	the	DT
cord-345088-krb1eidw	298	119	3b	3b	NNP
cord-345088-krb1eidw	298	120	protein	protein	NN
cord-345088-krb1eidw	298	121	in	in	IN
cord-345088-krb1eidw	298	122	pathogenesis	pathogenesis	NN
cord-345088-krb1eidw	298	123	and	and	CC
cord-345088-krb1eidw	298	124	replication	replication	NN
cord-345088-krb1eidw	298	125	Rearrangement	rearrangement	NN
cord-345088-krb1eidw	298	126	of	of	IN
cord-345088-krb1eidw	298	127	the	the	DT
cord-345088-krb1eidw	298	128	VP6	VP6	NNP
cord-345088-krb1eidw	298	129	gene	gene	NN
cord-345088-krb1eidw	298	130	of	of	IN
cord-345088-krb1eidw	298	131	a	a	DT
cord-345088-krb1eidw	298	132	group	group	NN
cord-345088-krb1eidw	298	133	A	a	DT
cord-345088-krb1eidw	298	134	rotavirus	rotavirus	NN
cord-345088-krb1eidw	298	135	in	in	IN
cord-345088-krb1eidw	298	136	combination	combination	NN
cord-345088-krb1eidw	298	137	with	with	IN
cord-345088-krb1eidw	298	138	a	a	DT
cord-345088-krb1eidw	298	139	point	point	NN
cord-345088-krb1eidw	298	140	mutation	mutation	NN
cord-345088-krb1eidw	298	141	affecting	affect	VBG
cord-345088-krb1eidw	298	142	trimer	trimer	NN
cord-345088-krb1eidw	298	143	stability	stability	NN
cord-345088-krb1eidw	298	144	Sequence	sequence	NN
cord-345088-krb1eidw	298	145	analysis	analysis	NN
cord-345088-krb1eidw	298	146	and	and	CC
cord-345088-krb1eidw	298	147	in	in	IN
cord-345088-krb1eidw	298	148	vitro	vitro	FW
cord-345088-krb1eidw	298	149	expression	expression	NN
cord-345088-krb1eidw	298	150	of	of	IN
cord-345088-krb1eidw	298	151	genes	gene	NNS
cord-345088-krb1eidw	298	152	6	6	CD
cord-345088-krb1eidw	298	153	and	and	CC
cord-345088-krb1eidw	298	154	11	11	CD
cord-345088-krb1eidw	298	155	of	of	IN
cord-345088-krb1eidw	298	156	an	an	DT
cord-345088-krb1eidw	298	157	ovine	ovine	JJ
cord-345088-krb1eidw	298	158	group	group	NN
cord-345088-krb1eidw	298	159	B	B	NNP
cord-345088-krb1eidw	298	160	rotavirus	rotavirus	NN
cord-345088-krb1eidw	298	161	isolate	isolate	NN
cord-345088-krb1eidw	298	162	,	,	,
cord-345088-krb1eidw	298	163	KB63	KB63	NNP
cord-345088-krb1eidw	298	164	:	:	:
cord-345088-krb1eidw	298	165	evidence	evidence	NN
cord-345088-krb1eidw	298	166	for	for	IN
cord-345088-krb1eidw	298	167	a	a	DT
cord-345088-krb1eidw	298	168	non	non	JJ
cord-345088-krb1eidw	298	169	-	-	JJ
cord-345088-krb1eidw	298	170	defective	defective	JJ
cord-345088-krb1eidw	298	171	,	,	,
cord-345088-krb1eidw	298	172	C	c	NN
cord-345088-krb1eidw	298	173	-	-	,
cord-345088-krb1eidw	298	174	terminally	terminally	RB
cord-345088-krb1eidw	298	175	truncated	truncate	VBN
cord-345088-krb1eidw	298	176	NSP1	NSP1	NNP
cord-345088-krb1eidw	298	177	and	and	CC
cord-345088-krb1eidw	298	178	a	a	DT
cord-345088-krb1eidw	298	179	phosphorylated	phosphorylate	VBN
cord-345088-krb1eidw	298	180	NSP5	NSP5	NNP
cord-345088-krb1eidw	298	181	Proteolytic	Proteolytic	NNP
cord-345088-krb1eidw	298	182	cleavage	cleavage	NN
cord-345088-krb1eidw	298	183	of	of	IN
cord-345088-krb1eidw	298	184	the	the	DT
cord-345088-krb1eidw	298	185	E2	E2	NNP
cord-345088-krb1eidw	298	186	glycoprotein	glycoprotein	NN
cord-345088-krb1eidw	298	187	of	of	IN
cord-345088-krb1eidw	298	188	murine	murine	JJ
cord-345088-krb1eidw	298	189	coronavirus	coronavirus	NN
cord-345088-krb1eidw	298	190	:	:	:
cord-345088-krb1eidw	298	191	activation	activation	NN
cord-345088-krb1eidw	298	192	of	of	IN
cord-345088-krb1eidw	298	193	cell	cell	NN
cord-345088-krb1eidw	298	194	-	-	HYPH
cord-345088-krb1eidw	298	195	fusing	fuse	VBG
cord-345088-krb1eidw	298	196	activity	activity	NN
cord-345088-krb1eidw	298	197	of	of	IN
cord-345088-krb1eidw	298	198	virions	virion	NNS
cord-345088-krb1eidw	298	199	by	by	IN
cord-345088-krb1eidw	298	200	trypsin	trypsin	NN
cord-345088-krb1eidw	298	201	and	and	CC
cord-345088-krb1eidw	298	202	separation	separation	NN
cord-345088-krb1eidw	298	203	of	of	IN
cord-345088-krb1eidw	299	1	two	two	CD
cord-345088-krb1eidw	299	2	different	different	JJ
cord-345088-krb1eidw	299	3	90	90	CD
cord-345088-krb1eidw	299	4	K	k	NN
cord-345088-krb1eidw	299	5	cleavage	cleavage	NN
cord-345088-krb1eidw	299	6	fragments	fragment	NNS
cord-345088-krb1eidw	299	7	Analysis	analysis	NN
cord-345088-krb1eidw	299	8	of	of	IN
cord-345088-krb1eidw	299	9	the	the	DT
cord-345088-krb1eidw	299	10	receptor	receptor	NN
cord-345088-krb1eidw	299	11	-	-	HYPH
cord-345088-krb1eidw	299	12	binding	bind	VBG
cord-345088-krb1eidw	299	13	site	site	NN
cord-345088-krb1eidw	299	14	of	of	IN
cord-345088-krb1eidw	299	15	murine	murine	JJ
cord-345088-krb1eidw	299	16	coronavirus	coronavirus	NN
cord-345088-krb1eidw	299	17	spike	spike	NN
cord-345088-krb1eidw	299	18	protein	protein	NN
cord-345088-krb1eidw	299	19	Interactions	interaction	NNS
cord-345088-krb1eidw	299	20	between	between	IN
cord-345088-krb1eidw	299	21	newly	newly	RB
cord-345088-krb1eidw	299	22	synthesized	synthesize	VBN
cord-345088-krb1eidw	299	23	glycoproteins	glycoprotein	NNS
cord-345088-krb1eidw	299	24	,	,	,
cord-345088-krb1eidw	299	25	calnexin	calnexin	NN
cord-345088-krb1eidw	299	26	and	and	CC
cord-345088-krb1eidw	299	27	a	a	DT
cord-345088-krb1eidw	299	28	network	network	NN
cord-345088-krb1eidw	299	29	of	of	IN
cord-345088-krb1eidw	299	30	resident	resident	JJ
cord-345088-krb1eidw	299	31	chaperones	chaperone	NNS
cord-345088-krb1eidw	299	32	in	in	IN
cord-345088-krb1eidw	299	33	the	the	DT
cord-345088-krb1eidw	299	34	endoplasmic	endoplasmic	NN
cord-345088-krb1eidw	299	35	reticulum	reticulum	NN
cord-345088-krb1eidw	299	36	Replication	replication	NN
cord-345088-krb1eidw	299	37	of	of	IN
cord-345088-krb1eidw	299	38	coronavirus	coronavirus	NN
cord-345088-krb1eidw	299	39	MHV	mhv	NN
cord-345088-krb1eidw	299	40	-	-	HYPH
cord-345088-krb1eidw	299	41	A59	A59	NNP
cord-345088-krb1eidw	299	42	in	in	IN
cord-345088-krb1eidw	299	43	sac	sac	NN
cord-345088-krb1eidw	299	44	-	-	HYPH
cord-345088-krb1eidw	299	45	cells	cell	NNS
cord-345088-krb1eidw	299	46	:	:	:
cord-345088-krb1eidw	299	47	determination	determination	NN
cord-345088-krb1eidw	299	48	of	of	IN
cord-345088-krb1eidw	299	49	the	the	DT
cord-345088-krb1eidw	299	50	first	first	JJ
cord-345088-krb1eidw	299	51	site	site	NN
cord-345088-krb1eidw	299	52	of	of	IN
cord-345088-krb1eidw	299	53	budding	budding	NN
cord-345088-krb1eidw	299	54	of	of	IN
cord-345088-krb1eidw	299	55	progeny	progeny	JJ
cord-345088-krb1eidw	299	56	virions	virion	NNS
cord-345088-krb1eidw	300	1	A	a	DT
cord-345088-krb1eidw	300	2	12-amino	12-amino	CD
cord-345088-krb1eidw	300	3	acid	acid	NN
cord-345088-krb1eidw	300	4	stretch	stretch	NN
cord-345088-krb1eidw	300	5	in	in	IN
cord-345088-krb1eidw	300	6	the	the	DT
cord-345088-krb1eidw	300	7	hypervariable	hypervariable	JJ
cord-345088-krb1eidw	300	8	region	region	NN
cord-345088-krb1eidw	300	9	of	of	IN
cord-345088-krb1eidw	300	10	the	the	DT
cord-345088-krb1eidw	300	11	spike	spike	NN
cord-345088-krb1eidw	300	12	protein	protein	NN
cord-345088-krb1eidw	300	13	S1	s1	NN
cord-345088-krb1eidw	300	14	subunit	subunit	NN
cord-345088-krb1eidw	300	15	is	be	VBZ
cord-345088-krb1eidw	300	16	critical	critical	JJ
cord-345088-krb1eidw	300	17	for	for	IN
cord-345088-krb1eidw	300	18	cell	cell	NN
cord-345088-krb1eidw	300	19	fusion	fusion	NN
cord-345088-krb1eidw	300	20	activity	activity	NN
cord-345088-krb1eidw	300	21	of	of	IN
cord-345088-krb1eidw	300	22	mouse	mouse	NN
cord-345088-krb1eidw	300	23	hepatitis	hepatitis	NN
cord-345088-krb1eidw	300	24	virus	virus	NN
cord-345088-krb1eidw	300	25	Nucleocapsid	Nucleocapsid	NNP
cord-345088-krb1eidw	300	26	-	-	HYPH
cord-345088-krb1eidw	300	27	independent	independent	JJ
cord-345088-krb1eidw	300	28	assembly	assembly	NN
cord-345088-krb1eidw	300	29	of	of	IN
cord-345088-krb1eidw	300	30	coronavirus	coronavirus	NN
cord-345088-krb1eidw	300	31	-	-	HYPH
cord-345088-krb1eidw	300	32	like	like	JJ
cord-345088-krb1eidw	300	33	particles	particle	NNS
cord-345088-krb1eidw	300	34	by	by	IN
cord-345088-krb1eidw	300	35	co	co	NN
cord-345088-krb1eidw	300	36	-	-	NN
cord-345088-krb1eidw	300	37	expression	expression	NN
cord-345088-krb1eidw	300	38	of	of	IN
cord-345088-krb1eidw	300	39	viral	viral	JJ
cord-345088-krb1eidw	300	40	envelope	envelope	NN
cord-345088-krb1eidw	300	41	protein	protein	NN
cord-345088-krb1eidw	300	42	genes	gene	NNS
cord-345088-krb1eidw	301	1	This	this	DT
cord-345088-krb1eidw	301	2	work	work	NN
cord-345088-krb1eidw	301	3	was	be	VBD
cord-345088-krb1eidw	301	4	supported	support	VBN
cord-345088-krb1eidw	301	5	by	by	IN
cord-345088-krb1eidw	301	6	the	the	DT
cord-345088-krb1eidw	301	7	Biomedical	Biomedical	NNP
cord-345088-krb1eidw	301	8	Research	Research	NNP
cord-345088-krb1eidw	301	9	Council	Council	NNP
cord-345088-krb1eidw	301	10	,	,	,
cord-345088-krb1eidw	301	11	Agency	Agency	NNP
cord-345088-krb1eidw	301	12	for	for	IN
cord-345088-krb1eidw	301	13	Science	Science	NNP
cord-345088-krb1eidw	301	14	Technology	Technology	NNP
cord-345088-krb1eidw	301	15	and	and	CC
cord-345088-krb1eidw	301	16	Research	Research	NNP
cord-345088-krb1eidw	301	17	,	,	,
cord-345088-krb1eidw	301	18	Singapore	Singapore	NNP
cord-345088-krb1eidw	301	19	.	.	.