key: cord-330847-a84pcc9z authors: Edwards, M. C.; Petty, I.T.D.; Jackson, A. O. title: RNA recombination in the genome of Barley stripe mosaic virus date: 1992-07-31 journal: Virology DOI: 10.1016/0042-6822(92)90722-2 sha: doc_id: 330847 cord_uid: a84pcc9z Abstract Barley stripe mosaic Hordeivirus (BSMV) is a positive-strand RNA virus requiring three single-stranded RNAs (α, β, and γ) for infectivity. A terminal-sequence-dependent cloning strategy was used to clone the entire genome of the CV17 strain. Full-length γ cDNA clones were obtained when oligonucleotides specific for the 5′-terminal sequence of RNA α were used in the cloning procedure, but not when RNA γ-specific oligonucleotides were used. Sequence analysis of six putative γ cDNA clones revealed that nucleotides 1–70 possess 89% homology with the first 70 nucleotides of RNA α. This leader region is separated from the γ-specific coding region by an eight-base intervening sequence common to both CV17 RNAs α and γ. Northern and Southern hybridization with oligonucleotide probes specific for either α or γ leader sequences indicated that CV17 γ cDNA clones are representative of native CV17 γ RNAs. Furthermore, bioassays indicated that in vitro transcripts derived from these γ cDNA clones were infectious when coinoculated with in vitro transcripts of full-length α and β cDNA clones. Thus, the evidence suggests that RNA γ of BSMV strain CV17 is a recombinant molecule which may have arisen as a result of natural recombination between RNAs α and γ. Barley stripe mosaic virus (BSMV), a Hordeivirus, encodes seven major polypeptides in a segmented genome composed of three (+)-sense, single-stranded (ss) RNAs designated (Y (3.8 kb), p(3.3 kb), and y (3.2 or 2.8 kb) (I). The length of RNA y varies with the presence or absence of a tandem repeat near its 5' terminus. RNAs LY and y are able to replicate in protoplasts in the absence of RNA fl (Z), although the presence of all three RNAs is essential for infection of intact plants (3). Nucleotide sequence analyses confirm that the cua and ya genes encode the essential viral-derived replicase components (4, 5) . Although the sequence of each RNA is largely unique, the 3'-terminus of each RNA is composed of a highly conserved sequence that can be folded into a tRNA-like structure and aminoacylated with tyrosine in viva (6). A "bandaid" cloning strategy, which exploits the conservation of the 3' terminal sequence, as well as the unique nature of the 5' ends of the BSMV RNAs, has been used previously to obtain full-length cDNA clones from which infectious in vitro transcripts can be produced (3, 7). We have now used this strategy to clone the entire genome of BSMV strain CV17. Analysis of the resulting cDNA clones suggested that CV17 RNA y is a naturally occurring chimeric recombinant, composed of a 70nucleotide (nt) a-specific leader sequence preceding a r-specific coding region. Further-' To whom reprint requests should be addressed. more, hybridization and bioassay data indicate that these clones are representative of native CV17 y RNAs. One of these CV17 y cDNA clones has been used previously to investigate the effects of short ORFs in the 5' leader of RNA y on the systemic movement of BSMV in Mcotiana benthamiana (8). The present paper provides evidence for the recombinant nature of CV17 RNA y. BSMV strain CV17 was propagated in barley (Hordeum vulgare cv. "Black Hulless," C.I. 666) and purified as previously reported (9). Virion RNA was extracted using a phenol-SDS procedure and was subsequently recovered by ethanol precipitation, followed by 3 A.4 sodium acetate precipitation, and finally by ethanol precipitation again (7). This mixture of RNAs (Y, p, and y was then used for cDNA synthesis and cloning as previously described for the ND1 8 and Type strains of BSMV (7). Briefly, linearized single-stranded phagemid DNA with a T7 promoter sequence at its 5'-end and a short 3'-terminal sequence complementary to the 3'-end of BSMV RNA was used as a primer for firststrand cDNA synthesis. Phagemid-linked full-length cDNA was then circularized by annealing to a bandaid oligonucleotide with sequence complementary to both ends of the phagemid:cDNA. The use of three different bandaid oligonucleotides, each with 5'-ends complementary to the T7 promoter sequence and with 3'-ends complementary to the 5'-ends of either full-length (Y, p, or y cDNAs, provided a means of isolating specific fulllength cDNA clones in a single cloning operation with- out the need for prior separation of the (Y, 0, and y RNAs. Full-length clones were initially identified by colony color and gel electrophoresis of ssDNA obtained after superinfection of transformed Escherichia co/i strain DH5aF' with bacteriophage Ml 3K07, as described previously (7). The full-length nature of individual clones and the integrity of the T7 promoter/BSMV 5'-end junction were verified by dideoxynucleotide sequencing of ssDNA templates (10). Full-length CV17 (Y and p cDNA clones were successfully isolated using the CY and ,f3 bandaid oligonucleotides, respectively. However, all attempts to isolate full-length y clones using the y bandaid oligonucleotide were unsuccessful. Instead, the initial size screening of cDNA clones obtained with the (Y bandaid oligonucleotide resulted in the identification of one with an insert equivalent in size to a 3.2-kb y RNA rather than a full-length (Y RNA (3.8 kb). Fourteen additional clones with inserts of this size were identified in subsequent cloning experiments utilizing the (Y bandaid oligonucleotide, whereas only one full-length (Y clone was isolated. Analysis of Rsal RFLPs in these fifteen putative y-specific CV17 cDNA clones indicated that all were similar to each other, but that they differed from the BSMV Type or ND1 8 strain y cDNA clones previously isolated by Petty et a/. (3) (Fig. 1) . Using an M 13 universal primer, six of these putative CV17 y clones were then sequenced in the region of the cDNA insert corresponding to the 5'-end of CV17 RNA y. A CV17 (Y clone was sequenced in a similar manner and also by direct RNA sequencing with reverse transcriptase (4) and a primer which bound to nt 195-209 (5'-CGTGCGAGCA-CAGTA). The sequences of all six y clones were identical for at least the first 100 nt and the first 70 nt of these cDNAs matched the 5'-terminal70 nt of CV17 RNA CI in 62 positions (Fig. 2) . However, no significant sequence alignment could be made between this region of the putative CV17 y cDNA clones and the first 70 nt of the Type strain RNA y. The 5' terminal 70 nt of the a-specific sequence in the CV17 y cDNAs were followed by an eight-base segment common to both CV17 a! and 7 RNAs. This sequence was, in turn, followed by a region that is identical to the sequence reported (4) for the Type strain RNA y for at least an additional 100 nt (Fig. 2) . Since the CV17 RNA y contains a ca. 370-nt tandem repeat near its 5'-terminus similar to that described for the Type strain (4) and a variant of the ND1 8 strain (3) the authenticity of the CV17 y cDNA clones was not readily verifiable by direct RNA sequencing. Instead, the authenticity of these clones was assessed by hybridization with oligonucleotide probes specific for CV17 (Y and Type y leader sequences. Northern blots of native RNAs of BSMV strains CV17, CV42, and ND1 8 and Southern blots of the fifteen CV17 y cDNA clones were prepared according to standard protocols (1 I). Blots were probed with either an QI leader-specific (5'~AAGAATCGATTACGATTATG) or a y leader-specific (5'-TlTAGCGTTATlTGGCAAGC) 32P-end-labeled oligonucleotide probe complementary to nt 41-60 of the CV17 (Y and Type y RNAs, respectively. Results evident in Fig. 3 demonstrate that native CV17 y RNAs typically possess an (Y leader sequence, as do all fifteen of the CV17 y cDNA clones. Further verification of the authenticity of the CV17 y cDNA clones was obtained by infectivity assay. Capped RNAs were produced by in vitro transcription of four of the CV17 y cDNA clones exactly as previously described (3). Each was combined with in vitro transcripts of either CV17 or ND1 8 (Y and CV17 p cDNA clones and inoculated onto 5-to 6-day-old barley seedlings. Inoculated seedlings developed symptoms typical of BSMV infection within 4 to 7 days, thus verifying that these CV17 y cDNA clones represent full-length and biologically active copies of CV17 RNA y. (Table 1) Our results strongly suggest that RNA y of BSMV strain CV17 is a naturally occurring product of recombination between RNAs (Y and y. Although the mechanism of recombination in BSMV is not known, two possible models for RNA recombination have been commonly proposed: (I) template switching (copy choice) and (II) enzymatic cutting/splicing. Kirkegaard and Baltimore (12) provided convincing evidence for a copy choice mechanism of poliovirus recombination; it appears likely that the recombination reported here proceeded in a similar manner. Although template switching may occur in the absence of sequence homology, it should be noted that a short homologous sequence at 10 20 30 40 50 60 70 80 90 100 110 120 130 cv17a CUAUGUAAGUUGCCUUUGGG UGU AAAAUUUCUUGCAUGCACAUAAUCGUAA UCGAUUCUUCUUGAUCUCUAAACAACACUUUCCCGUUAGC~GCUAGCGAUGAGAUUGUCCGCAAUCUGAUCUCCCGUGAGAGGU IIIIIIIIIIIIIIIIII I II IIIII IIII III1 IIIIIIIIIII llIIIIllI IIIIIII IIIIIIIII IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIlIIIIIIIIIIIIIIIIIIIIIIII TYPE, GUAUAGCUUGAGCAUUACCGUCGUGUAAUUGCAACACUUGGCUUGCCAAAUAACGCUAAAGCGUUCACGAAACAAACAACAAUUCGGC~GAUGUUGUGAAGAAAUUCGCCGUCAUGUCAGUGACUGUAGUAGCAGGUC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 FIG. 2. Comparison of the 5'termrnal sequence of barley stripe mosarc vrrus strarn CV17 RNA y wrth that of CV17 RNA CY and Type strain RNA y. Initiation codons for the cya and ya coding regions are underlrned. Vertical bars denote homology between sequences belreved to be evolutionarily related. Asterisks denote nucleotide matches that may have ansen by chance between molecules believed to be distinct. the putative crossover point is present in CV17 RNAs a and y, but not in RNA p. The presence of a tandem repeat in CV17 RNA y which is similar to the 372-nt tandem repeat present in the Type strain y is also suggestive of a copy choice mechanism. We have no conclusive explanation for the minor differences observed between the CV17 01 and y leader sequences. Considering the high degree of sequence conservation among BSMV strains (e.g., the (Y leader sequences of the Type and CV17 strains differ by only 1 nt), the sequence divergence between the CV17 (Y and y leaders may be due to selection for ability to replicate rather than random drift. The normal accumulation of RNA y to much higher levels than RNA QL in all strains studied thus far, including CV17, is an indication that differences in replication efficiency do exist among these RNAs (Fig. 3) . Alternatively, natural selection pressures on RNA N may not apply to RNA y, hence allowing genetic drift of the two leader se-quences. As a result of this divergence, only one of the two small overlapping ORFs present in the CV17 y leader is present in the CV17 N leader. It has been shown that the small y leader ORFs play a significant role in the efficiency of in vitro translation of the CV17 ya gene and can affect systemic movement and pathogenicity of ND1 8KV17 pseudorecombinants in N. benthamiana (8). However, the N origin of these small leader ORFs is not significant, since the Type strain y leader also contains a small ORF with unrelated sequence that has a similar effect on translation and systemic movement (8). Thus, phenotypic effects, if any, resulting from recombination of the CV17 RNA 01 and y leader regions have not yet been observed. Substantial evidence for the occurrence of RNA recombination now exists. Recombination has been well documented in RNA viruses, especially the picornavrruses, coronavrruses, and alphaviruses (12-15). Altered pathogenicity as a result of rncorporation of host 1 2 3 4 5 6 7 8 9 1011 12 1314 151617 18 FIG. 3. ldentrfrcatron of barley stripe mosaic virus strarn CV17 LY and Type strain y-specific leader sequences In 15 putative CV17 y cDNA clones and native RNAs of strains CV17, CV42, and ND1 8. (A) Southern blot of 15 putative BSMV strain CV17 7 cDNA clones (lanes 1-l 5), a Type strain y cDNA clone (lane 16) , and a CV17 u cDNA clone (lanes 17-l 8) hybndrzed to an LY leader-specrfrc olrgonucleotrde probe. Plasmrd DNAs in lanes l-l 7 were lrneanzed with il/l/ul. (B) Southern blot identical to A, except that the olrgonucleotide probe used was y leader-specific. (C) Northern blot of natrve RNAs from BSMV strains CV17, CV42, and ND1 8 hybridized to the same (Y leader-specrfic probe as In A. They RNAs of CV17 and CV42 are each 3.2 kb in size and comrgrate with RNA fi (arrow labeled fly); ND1 8 RNA y IS 2.8 kb In size (arrow labeled y). (0) Northern blot Identical to C, except that the y leader-specific olrgonucleotrde probe was used. Hybrrdrzatrons were performed In 6X SSPE t I % SDS at 54" for the N leader-specific probe and at 58" for the y leader-specific probe (T, = T,, -9"). a Black Hulless barley plants were inoculated with a mixture of in vitro transcripts from cDNA clones of both the ND18 and CV17 strains suspended in GKP buffer (50 mM glycine, 30 mM K,HPO, pH 9.2, 1% bentonite, 1% celite). The number of infected plants was determined by visual inspection. No symptoms were observed on control plants mock inoculated with GKP buffer alone Proc. Nat Other examples, such as the deletion of repeated sequences in tobacco mosaic virus mutants, have been reported more recently (19) Proc. Nat/. Acad. Sci. USA Molecular Cloning: A Laboratory Manual The high degree of nucleotide sequence similarity between tomato ringspot nepovirus RNAs 1 and 2 also may be a result of recombination (26). Recently, recombination was found to have occurred between turnip crinkle virus genomic RNA and satellite RNA D Retroviruses, Viroids, and RNA Recombination New Aspects of Positive Strand RNA Viruses ACKNOWLEDGMENTS 25 This work was supported in part by USDA Competitive Grant 26