id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263489-i4tkdgy4	Suo, Siqingaowa	Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential	2015-05-27		.txt	text/plain	2211	130	65	title: Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential Herein, we use similar technology and advance previous work by using the rS-AD as an immobilizing target to select phages from a peptide display library, with diagnostic potential for TGEV. Our results indicate that phages bearing peptide ligands that bind rS-AD can be used to develop a phage-mediated ELISA with high sensitivity and specificity to distinguish TGEV from other common swine viruses. To compare the sensitivities of phage-mediated ELISA to antibody-mediated ELISA, TGEV serially diluted in 0.1 M NaHCO 3 (pH 8.6) was coated onto duplicate ELISA plates overnight at 4°C followed by blocking with 5 % skim milk for 3 h at rt. Predicted amino acid sequences were generated for ten selected phages In summary, we identified peptides that specifically bind to TGEV and can form the basis of new diagnostic tests where the sensitivity of phTGEV-SAD15 was 0.1 lg of TGEV.	./cache/cord-263489-i4tkdgy4.txt	./txt/cord-263489-i4tkdgy4.txt