id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311204-fc12f845	Zhou, Ling	Full-length genomic characterization and molecular evolution of canine parvovirus in China	2016-04-02		.txt	text/plain	2229	138	65	Canine parvovirus type 2 (CPV-2) can cause acute haemorrhagic enteritis in dogs and myocarditis in puppies. Some faecal samples were negative for the CPV-2 antigen based on a colloidal gold test strip but were positive based on PCR, and a viral strain was isolated from one such sample. Identification of CPV-2 with cell culture and IPMA Feline kidney cell line F81 was obtained from the American Type Culture Collection, USA and was used to isolate viruses from clinical samples and to observe cytopathic effects associated with viral replication. Cloning the full-length genomic sequence of CPV-2 DNA and RNA were extracted from the homogenized samples (faeces from infected dogs) with the TIANamp Virus DNA/RNA Kit (Beijing TIANGEN Biotech Company, Beijing, China) according to the manufacturer's protocol. One viral strain was isolated from the faeces of dogs that tested negative in the colloidal gold test strip but positive with PCR.	./cache/cord-311204-fc12f845.txt	./txt/cord-311204-fc12f845.txt