id sid tid token lemma pos cord-005207-02wmt2e9 1 1 key key NN cord-005207-02wmt2e9 1 2 : : : cord-005207-02wmt2e9 2 1 cord-005207 cord-005207 NNP cord-005207-02wmt2e9 2 2 - - HYPH cord-005207-02wmt2e9 2 3 02wmt2e9 02wmt2e9 CD cord-005207-02wmt2e9 2 4 authors author NNS cord-005207-02wmt2e9 2 5 : : : cord-005207-02wmt2e9 2 6 Lee Lee NNP cord-005207-02wmt2e9 2 7 , , , cord-005207-02wmt2e9 2 8 Hee Hee NNP cord-005207-02wmt2e9 2 9 - - HYPH cord-005207-02wmt2e9 2 10 Kyung Kyung NNP cord-005207-02wmt2e9 2 11 ; ; : cord-005207-02wmt2e9 2 12 Yeo Yeo NNP cord-005207-02wmt2e9 2 13 , , , cord-005207-02wmt2e9 2 14 Sang Sang NNP cord-005207-02wmt2e9 2 15 - - HYPH cord-005207-02wmt2e9 2 16 Geon Geon NNP cord-005207-02wmt2e9 2 17 title title NN cord-005207-02wmt2e9 2 18 : : : cord-005207-02wmt2e9 2 19 Cloning cloning NN cord-005207-02wmt2e9 2 20 and and CC cord-005207-02wmt2e9 2 21 Sequence Sequence NNP cord-005207-02wmt2e9 2 22 Analysis Analysis NNP cord-005207-02wmt2e9 2 23 of of IN cord-005207-02wmt2e9 2 24 the the DT cord-005207-02wmt2e9 2 25 Nucleocapsid Nucleocapsid NNP cord-005207-02wmt2e9 2 26 Gene Gene NNP cord-005207-02wmt2e9 2 27 of of IN cord-005207-02wmt2e9 2 28 Porcine Porcine NNP cord-005207-02wmt2e9 2 29 Epidemic Epidemic NNP cord-005207-02wmt2e9 2 30 Diarrhea Diarrhea NNP cord-005207-02wmt2e9 2 31 Virus virus NN cord-005207-02wmt2e9 2 32 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 2 33 date date NN cord-005207-02wmt2e9 2 34 : : : cord-005207-02wmt2e9 3 1 2003 2003 CD cord-005207-02wmt2e9 3 2 journal journal NNP cord-005207-02wmt2e9 3 3 : : : cord-005207-02wmt2e9 4 1 Virus Virus NNP cord-005207-02wmt2e9 4 2 Genes Genes NNP cord-005207-02wmt2e9 4 3 DOI DOI NNP cord-005207-02wmt2e9 4 4 : : : cord-005207-02wmt2e9 5 1 10.1023 10.1023 CD cord-005207-02wmt2e9 5 2 / / SYM cord-005207-02wmt2e9 5 3 a:1023447732567 a:1023447732567 NNP cord-005207-02wmt2e9 5 4 sha sha NNP cord-005207-02wmt2e9 5 5 : : : cord-005207-02wmt2e9 6 1 1ec99dbd97c91ef0df27fe4104678405d91c1bc6 1ec99dbd97c91ef0df27fe4104678405d91c1bc6 CD cord-005207-02wmt2e9 6 2 doc_id doc_id CD cord-005207-02wmt2e9 6 3 : : : cord-005207-02wmt2e9 7 1 5207 5207 CD cord-005207-02wmt2e9 7 2 cord_uid cord_uid NNS cord-005207-02wmt2e9 7 3 : : : cord-005207-02wmt2e9 7 4 02wmt2e9 02wmt2e9 CD cord-005207-02wmt2e9 8 1 The the DT cord-005207-02wmt2e9 8 2 nucleocapsid nucleocapsid NN cord-005207-02wmt2e9 8 3 ( ( -LRB- cord-005207-02wmt2e9 8 4 N N NNP cord-005207-02wmt2e9 8 5 ) ) -RRB- cord-005207-02wmt2e9 8 6 gene gene NN cord-005207-02wmt2e9 8 7 of of IN cord-005207-02wmt2e9 8 8 the the DT cord-005207-02wmt2e9 8 9 porcine porcine JJ cord-005207-02wmt2e9 8 10 epidemic epidemic NN cord-005207-02wmt2e9 8 11 diarrhea diarrhea NN cord-005207-02wmt2e9 8 12 virus virus NN cord-005207-02wmt2e9 8 13 ( ( -LRB- cord-005207-02wmt2e9 8 14 PEDV PEDV NNP cord-005207-02wmt2e9 8 15 ) ) -RRB- cord-005207-02wmt2e9 8 16 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 8 17 which which WDT cord-005207-02wmt2e9 8 18 was be VBD cord-005207-02wmt2e9 8 19 previously previously RB cord-005207-02wmt2e9 8 20 isolated isolate VBN cord-005207-02wmt2e9 8 21 in in IN cord-005207-02wmt2e9 8 22 Chinju Chinju NNP cord-005207-02wmt2e9 8 23 , , , cord-005207-02wmt2e9 8 24 Korea Korea NNP cord-005207-02wmt2e9 8 25 was be VBD cord-005207-02wmt2e9 8 26 cloned clone VBN cord-005207-02wmt2e9 8 27 and and CC cord-005207-02wmt2e9 8 28 sequenced sequence VBN cord-005207-02wmt2e9 8 29 to to TO cord-005207-02wmt2e9 8 30 establish establish VB cord-005207-02wmt2e9 8 31 the the DT cord-005207-02wmt2e9 8 32 information information NN cord-005207-02wmt2e9 8 33 for for IN cord-005207-02wmt2e9 8 34 the the DT cord-005207-02wmt2e9 8 35 development development NN cord-005207-02wmt2e9 8 36 of of IN cord-005207-02wmt2e9 8 37 genetically genetically RB cord-005207-02wmt2e9 8 38 engineered engineer VBN cord-005207-02wmt2e9 8 39 diagnostic diagnostic JJ cord-005207-02wmt2e9 8 40 reagents reagent NNS cord-005207-02wmt2e9 8 41 . . . cord-005207-02wmt2e9 9 1 Also also RB cord-005207-02wmt2e9 9 2 , , , cord-005207-02wmt2e9 9 3 sequences sequence NNS cord-005207-02wmt2e9 9 4 of of IN cord-005207-02wmt2e9 9 5 the the DT cord-005207-02wmt2e9 9 6 nucleotides nucleotide NNS cord-005207-02wmt2e9 9 7 and and CC cord-005207-02wmt2e9 9 8 deduced deduce VBN cord-005207-02wmt2e9 9 9 amino amino JJ cord-005207-02wmt2e9 9 10 acids acid NNS cord-005207-02wmt2e9 9 11 of of IN cord-005207-02wmt2e9 9 12 the the DT cord-005207-02wmt2e9 9 13 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 10 1 N n NN cord-005207-02wmt2e9 10 2 gene gene NN cord-005207-02wmt2e9 10 3 were be VBD cord-005207-02wmt2e9 10 4 analyzed analyze VBN cord-005207-02wmt2e9 10 5 by by IN cord-005207-02wmt2e9 10 6 alignment alignment NN cord-005207-02wmt2e9 10 7 with with IN cord-005207-02wmt2e9 10 8 those those DT cord-005207-02wmt2e9 10 9 of of IN cord-005207-02wmt2e9 10 10 CV777 CV777 NNP cord-005207-02wmt2e9 10 11 and and CC cord-005207-02wmt2e9 10 12 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 10 13 . . . cord-005207-02wmt2e9 11 1 The the DT cord-005207-02wmt2e9 11 2 nucleotide nucleotide JJ cord-005207-02wmt2e9 11 3 sequence sequence NN cord-005207-02wmt2e9 11 4 encoding encode VBG cord-005207-02wmt2e9 11 5 the the DT cord-005207-02wmt2e9 11 6 entire entire JJ cord-005207-02wmt2e9 11 7 N n CD cord-005207-02wmt2e9 11 8 gene gene NN cord-005207-02wmt2e9 11 9 open open JJ cord-005207-02wmt2e9 11 10 reading reading NN cord-005207-02wmt2e9 11 11 frame frame NN cord-005207-02wmt2e9 11 12 ( ( -LRB- cord-005207-02wmt2e9 11 13 ORF ORF NNP cord-005207-02wmt2e9 11 14 ) ) -RRB- cord-005207-02wmt2e9 11 15 of of IN cord-005207-02wmt2e9 11 16 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 12 1 was be VBD cord-005207-02wmt2e9 12 2 1326 1326 CD cord-005207-02wmt2e9 12 3 bases basis NNS cord-005207-02wmt2e9 12 4 long long RB cord-005207-02wmt2e9 12 5 and and CC cord-005207-02wmt2e9 12 6 encoded encode VBD cord-005207-02wmt2e9 12 7 a a DT cord-005207-02wmt2e9 12 8 protein protein NN cord-005207-02wmt2e9 12 9 of of IN cord-005207-02wmt2e9 12 10 441 441 CD cord-005207-02wmt2e9 12 11 amino amino JJ cord-005207-02wmt2e9 12 12 acids acid NNS cord-005207-02wmt2e9 12 13 with with IN cord-005207-02wmt2e9 12 14 predicted predict VBN cord-005207-02wmt2e9 12 15 M M NNP cord-005207-02wmt2e9 12 16 ( ( -LRB- cord-005207-02wmt2e9 12 17 r r NN cord-005207-02wmt2e9 12 18 ) ) -RRB- cord-005207-02wmt2e9 12 19 of of IN cord-005207-02wmt2e9 12 20 49 49 CD cord-005207-02wmt2e9 12 21 kDa kDa NNS cord-005207-02wmt2e9 12 22 . . . cord-005207-02wmt2e9 13 1 It -PRON- PRP cord-005207-02wmt2e9 13 2 consisted consist VBD cord-005207-02wmt2e9 13 3 of of IN cord-005207-02wmt2e9 13 4 405 405 CD cord-005207-02wmt2e9 13 5 adenine adenine NN cord-005207-02wmt2e9 13 6 ( ( -LRB- cord-005207-02wmt2e9 13 7 30.5 30.5 CD cord-005207-02wmt2e9 13 8 % % NN cord-005207-02wmt2e9 13 9 ) ) -RRB- cord-005207-02wmt2e9 13 10 , , , cord-005207-02wmt2e9 13 11 293 293 CD cord-005207-02wmt2e9 13 12 cytosine cytosine NN cord-005207-02wmt2e9 13 13 ( ( -LRB- cord-005207-02wmt2e9 13 14 22.1 22.1 CD cord-005207-02wmt2e9 13 15 % % NN cord-005207-02wmt2e9 13 16 ) ) -RRB- cord-005207-02wmt2e9 13 17 , , , cord-005207-02wmt2e9 13 18 334 334 CD cord-005207-02wmt2e9 13 19 guanines guanine NNS cord-005207-02wmt2e9 13 20 ( ( -LRB- cord-005207-02wmt2e9 13 21 25.2 25.2 CD cord-005207-02wmt2e9 13 22 % % NN cord-005207-02wmt2e9 13 23 ) ) -RRB- cord-005207-02wmt2e9 13 24 and and CC cord-005207-02wmt2e9 13 25 294 294 CD cord-005207-02wmt2e9 13 26 thymines thymine NNS cord-005207-02wmt2e9 13 27 ( ( -LRB- cord-005207-02wmt2e9 13 28 22.2 22.2 CD cord-005207-02wmt2e9 13 29 % % NN cord-005207-02wmt2e9 13 30 ) ) -RRB- cord-005207-02wmt2e9 13 31 residues residue NNS cord-005207-02wmt2e9 13 32 . . . cord-005207-02wmt2e9 14 1 The the DT cord-005207-02wmt2e9 14 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 14 3 N N NNP cord-005207-02wmt2e9 14 4 ORF ORF NNP cord-005207-02wmt2e9 14 5 nucleotide nucleotide JJ cord-005207-02wmt2e9 14 6 sequence sequence NN cord-005207-02wmt2e9 14 7 was be VBD cord-005207-02wmt2e9 14 8 96.5 96.5 CD cord-005207-02wmt2e9 14 9 % % NN cord-005207-02wmt2e9 14 10 and and CC cord-005207-02wmt2e9 14 11 96.4 96.4 CD cord-005207-02wmt2e9 14 12 % % NN cord-005207-02wmt2e9 14 13 homologous homologous JJ cord-005207-02wmt2e9 14 14 with with IN cord-005207-02wmt2e9 14 15 that that DT cord-005207-02wmt2e9 14 16 of of IN cord-005207-02wmt2e9 14 17 the the DT cord-005207-02wmt2e9 14 18 CV777 CV777 NNP cord-005207-02wmt2e9 14 19 and and CC cord-005207-02wmt2e9 14 20 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 14 21 , , , cord-005207-02wmt2e9 14 22 respectively respectively RB cord-005207-02wmt2e9 14 23 . . . cord-005207-02wmt2e9 15 1 The the DT cord-005207-02wmt2e9 15 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 15 3 N N NNP cord-005207-02wmt2e9 15 4 protein protein NN cord-005207-02wmt2e9 15 5 revealed reveal VBD cord-005207-02wmt2e9 15 6 96.8 96.8 CD cord-005207-02wmt2e9 15 7 % % NN cord-005207-02wmt2e9 15 8 amino amino NN cord-005207-02wmt2e9 15 9 acid acid NN cord-005207-02wmt2e9 15 10 identity identity NN cord-005207-02wmt2e9 15 11 with with IN cord-005207-02wmt2e9 15 12 that that DT cord-005207-02wmt2e9 15 13 of of IN cord-005207-02wmt2e9 15 14 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 15 15 and and CC cord-005207-02wmt2e9 15 16 CV777 CV777 NNP cord-005207-02wmt2e9 15 17 , , , cord-005207-02wmt2e9 15 18 respectively respectively RB cord-005207-02wmt2e9 15 19 . . . cord-005207-02wmt2e9 16 1 The the DT cord-005207-02wmt2e9 16 2 amino amino NN cord-005207-02wmt2e9 16 3 acid acid NN cord-005207-02wmt2e9 16 4 sequence sequence NN cord-005207-02wmt2e9 16 5 contained contain VBD cord-005207-02wmt2e9 16 6 seven seven CD cord-005207-02wmt2e9 16 7 potential potential JJ cord-005207-02wmt2e9 16 8 sites site NNS cord-005207-02wmt2e9 16 9 for for IN cord-005207-02wmt2e9 16 10 threonine threonine NN cord-005207-02wmt2e9 16 11 ( ( -LRB- cord-005207-02wmt2e9 16 12 T)- t)- NN cord-005207-02wmt2e9 16 13 or or CC cord-005207-02wmt2e9 16 14 serine serine NN cord-005207-02wmt2e9 16 15 ( ( -LRB- cord-005207-02wmt2e9 16 16 S)-linked S)-linked NNP cord-005207-02wmt2e9 16 17 phosphorylation phosphorylation NN cord-005207-02wmt2e9 16 18 by by IN cord-005207-02wmt2e9 16 19 each each DT cord-005207-02wmt2e9 16 20 protein protein NN cord-005207-02wmt2e9 16 21 kinase kinase NN cord-005207-02wmt2e9 16 22 C c NN cord-005207-02wmt2e9 16 23 and and CC cord-005207-02wmt2e9 16 24 casein casein NN cord-005207-02wmt2e9 16 25 kinase kinase NN cord-005207-02wmt2e9 16 26 II II NNP cord-005207-02wmt2e9 16 27 . . . cord-005207-02wmt2e9 17 1 Porcine porcine JJ cord-005207-02wmt2e9 17 2 epidemic epidemic NN cord-005207-02wmt2e9 17 3 diarrhea diarrhea NN cord-005207-02wmt2e9 17 4 virus virus NN cord-005207-02wmt2e9 17 5 ( ( -LRB- cord-005207-02wmt2e9 17 6 PEDV PEDV NNP cord-005207-02wmt2e9 17 7 ) ) -RRB- cord-005207-02wmt2e9 17 8 causes cause VBZ cord-005207-02wmt2e9 17 9 an an DT cord-005207-02wmt2e9 17 10 acute acute JJ cord-005207-02wmt2e9 17 11 infection infection NN cord-005207-02wmt2e9 17 12 in in IN cord-005207-02wmt2e9 17 13 piglets piglet NNS cord-005207-02wmt2e9 17 14 of of IN cord-005207-02wmt2e9 17 15 1±2 1±2 CD cord-005207-02wmt2e9 17 16 weeks week NNS cord-005207-02wmt2e9 17 17 old old JJ cord-005207-02wmt2e9 17 18 , , , cord-005207-02wmt2e9 17 19 and and CC cord-005207-02wmt2e9 17 20 the the DT cord-005207-02wmt2e9 17 21 disease disease NN cord-005207-02wmt2e9 17 22 is be VBZ cord-005207-02wmt2e9 17 23 characterized characterize VBN cord-005207-02wmt2e9 17 24 by by IN cord-005207-02wmt2e9 17 25 severe severe JJ cord-005207-02wmt2e9 17 26 enteritis enteritis NN cord-005207-02wmt2e9 17 27 and and CC cord-005207-02wmt2e9 17 28 diarrhea diarrhea NN cord-005207-02wmt2e9 17 29 , , , cord-005207-02wmt2e9 17 30 leading lead VBG cord-005207-02wmt2e9 17 31 to to IN cord-005207-02wmt2e9 17 32 death death NN cord-005207-02wmt2e9 17 33 with with IN cord-005207-02wmt2e9 17 34 mortality mortality NN cord-005207-02wmt2e9 17 35 up up IN cord-005207-02wmt2e9 17 36 to to TO cord-005207-02wmt2e9 17 37 90 90 CD cord-005207-02wmt2e9 17 38 % % NN cord-005207-02wmt2e9 17 39 [ [ -LRB- cord-005207-02wmt2e9 17 40 1 1 CD cord-005207-02wmt2e9 17 41 , , , cord-005207-02wmt2e9 17 42 2 2 CD cord-005207-02wmt2e9 17 43 ] ] -RRB- cord-005207-02wmt2e9 17 44 . . . cord-005207-02wmt2e9 18 1 PEDV PEDV NNP cord-005207-02wmt2e9 18 2 is be VBZ cord-005207-02wmt2e9 18 3 a a DT cord-005207-02wmt2e9 18 4 member member NN cord-005207-02wmt2e9 18 5 of of IN cord-005207-02wmt2e9 18 6 the the DT cord-005207-02wmt2e9 18 7 genus genus NN cord-005207-02wmt2e9 18 8 coronavirus coronavirus NN cord-005207-02wmt2e9 18 9 of of IN cord-005207-02wmt2e9 18 10 the the DT cord-005207-02wmt2e9 18 11 family family NN cord-005207-02wmt2e9 18 12 Coronaviridae Coronaviridae NNP cord-005207-02wmt2e9 18 13 [ [ -LRB- cord-005207-02wmt2e9 18 14 3 3 CD cord-005207-02wmt2e9 18 15 ] ] -RRB- cord-005207-02wmt2e9 18 16 . . . cord-005207-02wmt2e9 19 1 The the DT cord-005207-02wmt2e9 19 2 genome genome NN cord-005207-02wmt2e9 19 3 consists consist VBZ cord-005207-02wmt2e9 19 4 of of IN cord-005207-02wmt2e9 19 5 a a DT cord-005207-02wmt2e9 19 6 single single JJ cord-005207-02wmt2e9 19 7 molecule molecule NN cord-005207-02wmt2e9 19 8 of of IN cord-005207-02wmt2e9 19 9 positive positive JJ cord-005207-02wmt2e9 19 10 - - HYPH cord-005207-02wmt2e9 19 11 sense sense NN cord-005207-02wmt2e9 19 12 , , , cord-005207-02wmt2e9 19 13 single single RB cord-005207-02wmt2e9 19 14 - - HYPH cord-005207-02wmt2e9 19 15 stranded strand VBN cord-005207-02wmt2e9 19 16 RNA RNA NNP cord-005207-02wmt2e9 19 17 , , , cord-005207-02wmt2e9 19 18 27±32 27±32 NNP cord-005207-02wmt2e9 19 19 kb kb NNP cord-005207-02wmt2e9 19 20 in in IN cord-005207-02wmt2e9 19 21 size size NN cord-005207-02wmt2e9 19 22 , , , cord-005207-02wmt2e9 19 23 which which WDT cord-005207-02wmt2e9 19 24 is be VBZ cord-005207-02wmt2e9 19 25 transcribed transcribe VBN cord-005207-02wmt2e9 19 26 into into IN cord-005207-02wmt2e9 19 27 a a DT cord-005207-02wmt2e9 19 28 nested nested JJ cord-005207-02wmt2e9 19 29 set set NN cord-005207-02wmt2e9 19 30 of of IN cord-005207-02wmt2e9 19 31 several several JJ cord-005207-02wmt2e9 19 32 3 3 CD cord-005207-02wmt2e9 19 33 0 0 CD cord-005207-02wmt2e9 19 34 -coterminal -coterminal JJ cord-005207-02wmt2e9 19 35 subgenomic subgenomic NNP cord-005207-02wmt2e9 19 36 mRNAs mRNAs NNPS cord-005207-02wmt2e9 19 37 for for IN cord-005207-02wmt2e9 19 38 the the DT cord-005207-02wmt2e9 19 39 production production NN cord-005207-02wmt2e9 19 40 of of IN cord-005207-02wmt2e9 19 41 structural structural JJ cord-005207-02wmt2e9 19 42 and and CC cord-005207-02wmt2e9 19 43 nonstructural nonstructural JJ cord-005207-02wmt2e9 19 44 proteins protein NNS cord-005207-02wmt2e9 19 45 [ [ -LRB- cord-005207-02wmt2e9 19 46 3 3 CD cord-005207-02wmt2e9 19 47 , , , cord-005207-02wmt2e9 19 48 4 4 CD cord-005207-02wmt2e9 19 49 ] ] -RRB- cord-005207-02wmt2e9 19 50 . . . cord-005207-02wmt2e9 20 1 Among among IN cord-005207-02wmt2e9 20 2 structural structural JJ cord-005207-02wmt2e9 20 3 proteins protein NNS cord-005207-02wmt2e9 20 4 of of IN cord-005207-02wmt2e9 20 5 the the DT cord-005207-02wmt2e9 20 6 virion virion NN cord-005207-02wmt2e9 20 7 , , , cord-005207-02wmt2e9 20 8 spike spike NN cord-005207-02wmt2e9 20 9 ( ( -LRB- cord-005207-02wmt2e9 20 10 S s NN cord-005207-02wmt2e9 20 11 ) ) -RRB- cord-005207-02wmt2e9 20 12 glycoprotein glycoprotein NN cord-005207-02wmt2e9 20 13 ( ( -LRB- cord-005207-02wmt2e9 20 14 180±220 180±220 CD cord-005207-02wmt2e9 20 15 kDa kDa NNP cord-005207-02wmt2e9 20 16 ) ) -RRB- cord-005207-02wmt2e9 20 17 plays play VBZ cord-005207-02wmt2e9 20 18 an an DT cord-005207-02wmt2e9 20 19 important important JJ cord-005207-02wmt2e9 20 20 role role NN cord-005207-02wmt2e9 20 21 in in IN cord-005207-02wmt2e9 20 22 the the DT cord-005207-02wmt2e9 20 23 attachment attachment NN cord-005207-02wmt2e9 20 24 of of IN cord-005207-02wmt2e9 20 25 the the DT cord-005207-02wmt2e9 20 26 virion virion NN cord-005207-02wmt2e9 20 27 on on IN cord-005207-02wmt2e9 20 28 the the DT cord-005207-02wmt2e9 20 29 host host NN cord-005207-02wmt2e9 20 30 's 's POS cord-005207-02wmt2e9 20 31 receptors receptor NNS cord-005207-02wmt2e9 20 32 and and CC cord-005207-02wmt2e9 20 33 penetration penetration NN cord-005207-02wmt2e9 20 34 into into IN cord-005207-02wmt2e9 20 35 the the DT cord-005207-02wmt2e9 20 36 intestinal intestinal JJ cord-005207-02wmt2e9 20 37 villous villous NN cord-005207-02wmt2e9 20 38 cells cell NNS cord-005207-02wmt2e9 20 39 by by IN cord-005207-02wmt2e9 20 40 fusion fusion NN cord-005207-02wmt2e9 20 41 . . . cord-005207-02wmt2e9 21 1 The the DT cord-005207-02wmt2e9 21 2 S S NNP cord-005207-02wmt2e9 21 3 glycoprotein glycoprotein NN cord-005207-02wmt2e9 21 4 also also RB cord-005207-02wmt2e9 21 5 induces induce VBZ cord-005207-02wmt2e9 21 6 the the DT cord-005207-02wmt2e9 21 7 production production NN cord-005207-02wmt2e9 21 8 of of IN cord-005207-02wmt2e9 21 9 neutralizing neutralize VBG cord-005207-02wmt2e9 21 10 antibodies antibody NNS cord-005207-02wmt2e9 21 11 in in IN cord-005207-02wmt2e9 21 12 the the DT cord-005207-02wmt2e9 21 13 host host NN cord-005207-02wmt2e9 21 14 [ [ -LRB- cord-005207-02wmt2e9 21 15 5±7 5±7 CD cord-005207-02wmt2e9 21 16 ] ] -RRB- cord-005207-02wmt2e9 21 17 , , , cord-005207-02wmt2e9 21 18 and and CC cord-005207-02wmt2e9 21 19 therefore therefore RB cord-005207-02wmt2e9 21 20 , , , cord-005207-02wmt2e9 21 21 is be VBZ cord-005207-02wmt2e9 21 22 an an DT cord-005207-02wmt2e9 21 23 important important JJ cord-005207-02wmt2e9 21 24 substance substance NN cord-005207-02wmt2e9 21 25 for for IN cord-005207-02wmt2e9 21 26 the the DT cord-005207-02wmt2e9 21 27 immunity immunity NN cord-005207-02wmt2e9 21 28 against against IN cord-005207-02wmt2e9 21 29 PEDV PEDV NNP cord-005207-02wmt2e9 21 30 . . . cord-005207-02wmt2e9 22 1 On on IN cord-005207-02wmt2e9 22 2 the the DT cord-005207-02wmt2e9 22 3 other other JJ cord-005207-02wmt2e9 22 4 hand hand NN cord-005207-02wmt2e9 22 5 , , , cord-005207-02wmt2e9 22 6 nucleocapsid nucleocapsid NN cord-005207-02wmt2e9 22 7 ( ( -LRB- cord-005207-02wmt2e9 22 8 N N NNP cord-005207-02wmt2e9 22 9 ) ) -RRB- cord-005207-02wmt2e9 22 10 protein protein NN cord-005207-02wmt2e9 22 11 ( ( -LRB- cord-005207-02wmt2e9 22 12 55±58 55±58 NNP cord-005207-02wmt2e9 22 13 kDa kDa NNP cord-005207-02wmt2e9 22 14 ) ) -RRB- cord-005207-02wmt2e9 22 15 is be VBZ cord-005207-02wmt2e9 22 16 known know VBN cord-005207-02wmt2e9 22 17 as as IN cord-005207-02wmt2e9 22 18 a a DT cord-005207-02wmt2e9 22 19 basic basic JJ cord-005207-02wmt2e9 22 20 phosphoprotein phosphoprotein NN cord-005207-02wmt2e9 22 21 associated associate VBN cord-005207-02wmt2e9 22 22 with with IN cord-005207-02wmt2e9 22 23 the the DT cord-005207-02wmt2e9 22 24 genome genome NN cord-005207-02wmt2e9 22 25 [ [ -LRB- cord-005207-02wmt2e9 22 26 1 1 CD cord-005207-02wmt2e9 22 27 , , , cord-005207-02wmt2e9 22 28 3 3 CD cord-005207-02wmt2e9 22 29 , , , cord-005207-02wmt2e9 22 30 8 8 CD cord-005207-02wmt2e9 22 31 , , , cord-005207-02wmt2e9 22 32 9 9 CD cord-005207-02wmt2e9 22 33 ] ] -RRB- cord-005207-02wmt2e9 22 34 , , , cord-005207-02wmt2e9 22 35 which which WDT cord-005207-02wmt2e9 22 36 can can MD cord-005207-02wmt2e9 22 37 be be VB cord-005207-02wmt2e9 22 38 the the DT cord-005207-02wmt2e9 22 39 target target NN cord-005207-02wmt2e9 22 40 for for IN cord-005207-02wmt2e9 22 41 the the DT cord-005207-02wmt2e9 22 42 accurate accurate JJ cord-005207-02wmt2e9 22 43 and and CC cord-005207-02wmt2e9 22 44 early early JJ cord-005207-02wmt2e9 22 45 diagnosis diagnosis NN cord-005207-02wmt2e9 22 46 of of IN cord-005207-02wmt2e9 22 47 PEDV PEDV NNP cord-005207-02wmt2e9 22 48 infection infection NN cord-005207-02wmt2e9 22 49 by by IN cord-005207-02wmt2e9 22 50 molecular molecular JJ cord-005207-02wmt2e9 22 51 techniques technique NNS cord-005207-02wmt2e9 22 52 . . . cord-005207-02wmt2e9 23 1 Cloning clone VBG cord-005207-02wmt2e9 23 2 and and CC cord-005207-02wmt2e9 23 3 nucleotide nucleotide JJ cord-005207-02wmt2e9 23 4 sequencing sequencing NN cord-005207-02wmt2e9 23 5 have have VBP cord-005207-02wmt2e9 23 6 been be VBN cord-005207-02wmt2e9 23 7 done do VBN cord-005207-02wmt2e9 23 8 on on IN cord-005207-02wmt2e9 23 9 these these DT cord-005207-02wmt2e9 23 10 genes gene NNS cord-005207-02wmt2e9 23 11 of of IN cord-005207-02wmt2e9 23 12 CV777 CV777 NNP cord-005207-02wmt2e9 23 13 and and CC cord-005207-02wmt2e9 23 14 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 23 15 strains strain NNS cord-005207-02wmt2e9 23 16 [ [ -LRB- cord-005207-02wmt2e9 23 17 5 5 CD cord-005207-02wmt2e9 23 18 , , , cord-005207-02wmt2e9 23 19 10 10 CD cord-005207-02wmt2e9 23 20 ] ] -RRB- cord-005207-02wmt2e9 23 21 . . . cord-005207-02wmt2e9 24 1 The the DT cord-005207-02wmt2e9 24 2 gene gene NN cord-005207-02wmt2e9 24 3 products product NNS cord-005207-02wmt2e9 24 4 can can MD cord-005207-02wmt2e9 24 5 be be VB cord-005207-02wmt2e9 24 6 the the DT cord-005207-02wmt2e9 24 7 feasible feasible JJ cord-005207-02wmt2e9 24 8 alternative alternative NN cord-005207-02wmt2e9 24 9 to to TO cord-005207-02wmt2e9 24 10 develop develop VB cord-005207-02wmt2e9 24 11 genetically genetically RB cord-005207-02wmt2e9 24 12 engineered engineer VBN cord-005207-02wmt2e9 24 13 vaccines vaccine NNS cord-005207-02wmt2e9 24 14 and and CC cord-005207-02wmt2e9 24 15 diagnostic diagnostic JJ cord-005207-02wmt2e9 24 16 reagents reagent NNS cord-005207-02wmt2e9 24 17 . . . cord-005207-02wmt2e9 25 1 Since since IN cord-005207-02wmt2e9 25 2 isolation isolation NN cord-005207-02wmt2e9 25 3 of of IN cord-005207-02wmt2e9 25 4 PEDV PEDV NNP cord-005207-02wmt2e9 25 5 in in IN cord-005207-02wmt2e9 25 6 Korea Korea NNP cord-005207-02wmt2e9 25 7 was be VBD cord-005207-02wmt2e9 25 8 first first RB cord-005207-02wmt2e9 25 9 reported report VBN cord-005207-02wmt2e9 25 10 in in IN cord-005207-02wmt2e9 25 11 1993 1993 CD cord-005207-02wmt2e9 25 12 [ [ -LRB- cord-005207-02wmt2e9 25 13 11 11 CD cord-005207-02wmt2e9 25 14 ] ] -RRB- cord-005207-02wmt2e9 25 15 , , , cord-005207-02wmt2e9 25 16 the the DT cord-005207-02wmt2e9 25 17 virus virus NN cord-005207-02wmt2e9 25 18 has have VBZ cord-005207-02wmt2e9 25 19 been be VBN cord-005207-02wmt2e9 25 20 one one CD cord-005207-02wmt2e9 25 21 of of IN cord-005207-02wmt2e9 25 22 the the DT cord-005207-02wmt2e9 25 23 major major JJ cord-005207-02wmt2e9 25 24 causes cause NNS cord-005207-02wmt2e9 25 25 for for IN cord-005207-02wmt2e9 25 26 the the DT cord-005207-02wmt2e9 25 27 death death NN cord-005207-02wmt2e9 25 28 of of IN cord-005207-02wmt2e9 25 29 suckling suckle VBG cord-005207-02wmt2e9 25 30 piglets piglet NNS cord-005207-02wmt2e9 25 31 in in IN cord-005207-02wmt2e9 25 32 pig pig NN cord-005207-02wmt2e9 25 33 farming farming NN cord-005207-02wmt2e9 25 34 . . . cord-005207-02wmt2e9 26 1 Park Park NNP cord-005207-02wmt2e9 26 2 et et FW cord-005207-02wmt2e9 26 3 al al NNP cord-005207-02wmt2e9 26 4 . . . cord-005207-02wmt2e9 27 1 [ [ -LRB- cord-005207-02wmt2e9 27 2 12 12 CD cord-005207-02wmt2e9 27 3 ] ] -RRB- cord-005207-02wmt2e9 27 4 cloned clone VBD cord-005207-02wmt2e9 27 5 a a DT cord-005207-02wmt2e9 27 6 DNA dna NN cord-005207-02wmt2e9 27 7 of of IN cord-005207-02wmt2e9 27 8 750 750 CD cord-005207-02wmt2e9 27 9 bases basis NNS cord-005207-02wmt2e9 27 10 from from IN cord-005207-02wmt2e9 27 11 N n CD cord-005207-02wmt2e9 27 12 gene gene NN cord-005207-02wmt2e9 27 13 of of IN cord-005207-02wmt2e9 27 14 the the DT cord-005207-02wmt2e9 27 15 viral viral JJ cord-005207-02wmt2e9 27 16 RNA RNA NNP cord-005207-02wmt2e9 27 17 in in IN cord-005207-02wmt2e9 27 18 swine swine NN cord-005207-02wmt2e9 27 19 feces fece NNS cord-005207-02wmt2e9 27 20 , , , cord-005207-02wmt2e9 27 21 but but CC cord-005207-02wmt2e9 27 22 no no DT cord-005207-02wmt2e9 27 23 further further JJ cord-005207-02wmt2e9 27 24 studies study NNS cord-005207-02wmt2e9 27 25 on on IN cord-005207-02wmt2e9 27 26 the the DT cord-005207-02wmt2e9 27 27 viral viral JJ cord-005207-02wmt2e9 27 28 isolation isolation NN cord-005207-02wmt2e9 27 29 and and CC cord-005207-02wmt2e9 27 30 gene gene NN cord-005207-02wmt2e9 27 31 cloning cloning NN cord-005207-02wmt2e9 27 32 have have VBP cord-005207-02wmt2e9 27 33 been be VBN cord-005207-02wmt2e9 27 34 reported report VBN cord-005207-02wmt2e9 27 35 . . . cord-005207-02wmt2e9 28 1 In in IN cord-005207-02wmt2e9 28 2 the the DT cord-005207-02wmt2e9 28 3 development development NN cord-005207-02wmt2e9 28 4 of of IN cord-005207-02wmt2e9 28 5 genetically genetically RB cord-005207-02wmt2e9 28 6 engineered engineer VBN cord-005207-02wmt2e9 28 7 proteins protein NNS cord-005207-02wmt2e9 28 8 for for IN cord-005207-02wmt2e9 28 9 diagnostic diagnostic JJ cord-005207-02wmt2e9 28 10 reagents reagent NNS cord-005207-02wmt2e9 28 11 against against IN cord-005207-02wmt2e9 28 12 PEDV PEDV NNP cord-005207-02wmt2e9 28 13 , , , cord-005207-02wmt2e9 28 14 molecular molecular JJ cord-005207-02wmt2e9 28 15 characterization characterization NN cord-005207-02wmt2e9 28 16 of of IN cord-005207-02wmt2e9 28 17 the the DT cord-005207-02wmt2e9 28 18 N n CD cord-005207-02wmt2e9 28 19 gene gene NN cord-005207-02wmt2e9 28 20 is be VBZ cord-005207-02wmt2e9 28 21 rudimental rudimental JJ cord-005207-02wmt2e9 28 22 that that WDT cord-005207-02wmt2e9 28 23 still still RB cord-005207-02wmt2e9 28 24 need need VBP cord-005207-02wmt2e9 28 25 further further JJ cord-005207-02wmt2e9 28 26 elucidation elucidation NN cord-005207-02wmt2e9 28 27 . . . cord-005207-02wmt2e9 29 1 PEDV PEDV NNP cord-005207-02wmt2e9 29 2 infections infection NNS cord-005207-02wmt2e9 29 3 occur occur VBP cord-005207-02wmt2e9 29 4 frequently frequently RB cord-005207-02wmt2e9 29 5 in in IN cord-005207-02wmt2e9 29 6 Korea Korea NNP cord-005207-02wmt2e9 29 7 , , , cord-005207-02wmt2e9 29 8 and and CC cord-005207-02wmt2e9 29 9 developmental developmental JJ cord-005207-02wmt2e9 29 10 efforts effort NNS cord-005207-02wmt2e9 29 11 should should MD cord-005207-02wmt2e9 29 12 be be VB cord-005207-02wmt2e9 29 13 geared gear VBN cord-005207-02wmt2e9 29 14 toward toward IN cord-005207-02wmt2e9 29 15 rapid rapid JJ cord-005207-02wmt2e9 29 16 diagnosis diagnosis NN cord-005207-02wmt2e9 29 17 and and CC cord-005207-02wmt2e9 29 18 control control NN cord-005207-02wmt2e9 29 19 of of IN cord-005207-02wmt2e9 29 20 the the DT cord-005207-02wmt2e9 29 21 disease disease NN cord-005207-02wmt2e9 29 22 . . . cord-005207-02wmt2e9 30 1 To to IN cord-005207-02wmt2e9 30 2 our -PRON- PRP$ cord-005207-02wmt2e9 30 3 knowledge knowledge NN cord-005207-02wmt2e9 30 4 , , , cord-005207-02wmt2e9 30 5 nucleotide nucleotide JJ cord-005207-02wmt2e9 30 6 sequences sequence NNS cord-005207-02wmt2e9 30 7 of of IN cord-005207-02wmt2e9 30 8 the the DT cord-005207-02wmt2e9 30 9 full full JJ cord-005207-02wmt2e9 30 10 - - HYPH cord-005207-02wmt2e9 30 11 length length NN cord-005207-02wmt2e9 30 12 N n CD cord-005207-02wmt2e9 30 13 gene gene NN cord-005207-02wmt2e9 30 14 of of IN cord-005207-02wmt2e9 30 15 Korean korean JJ cord-005207-02wmt2e9 30 16 PEDV PEDV NNP cord-005207-02wmt2e9 30 17 isolates isolate NNS cord-005207-02wmt2e9 30 18 have have VBP cord-005207-02wmt2e9 30 19 not not RB cord-005207-02wmt2e9 30 20 been be VBN cord-005207-02wmt2e9 30 21 reported report VBN cord-005207-02wmt2e9 30 22 . . . cord-005207-02wmt2e9 31 1 In in IN cord-005207-02wmt2e9 31 2 the the DT cord-005207-02wmt2e9 31 3 present present JJ cord-005207-02wmt2e9 31 4 study study NN cord-005207-02wmt2e9 31 5 , , , cord-005207-02wmt2e9 31 6 a a DT cord-005207-02wmt2e9 31 7 DNA dna NN cord-005207-02wmt2e9 31 8 clone clone NN cord-005207-02wmt2e9 31 9 was be VBD cord-005207-02wmt2e9 31 10 constructed construct VBN cord-005207-02wmt2e9 31 11 for for IN cord-005207-02wmt2e9 31 12 the the DT cord-005207-02wmt2e9 31 13 full full JJ cord-005207-02wmt2e9 31 14 - - HYPH cord-005207-02wmt2e9 31 15 length length NN cord-005207-02wmt2e9 31 16 N n CD cord-005207-02wmt2e9 31 17 gene gene NN cord-005207-02wmt2e9 31 18 open open JJ cord-005207-02wmt2e9 31 19 reading reading NN cord-005207-02wmt2e9 31 20 frame frame NN cord-005207-02wmt2e9 31 21 ( ( -LRB- cord-005207-02wmt2e9 31 22 ORF ORF NNP cord-005207-02wmt2e9 31 23 ) ) -RRB- cord-005207-02wmt2e9 31 24 of of IN cord-005207-02wmt2e9 31 25 PEDV PEDV NNP cord-005207-02wmt2e9 31 26 isolated isolate VBN cord-005207-02wmt2e9 31 27 in in IN cord-005207-02wmt2e9 31 28 Chinju Chinju NNP cord-005207-02wmt2e9 31 29 , , , cord-005207-02wmt2e9 31 30 Korea Korea NNP cord-005207-02wmt2e9 31 31 . . . cord-005207-02wmt2e9 32 1 The the DT cord-005207-02wmt2e9 32 2 complete complete JJ cord-005207-02wmt2e9 32 3 sequences sequence NNS cord-005207-02wmt2e9 32 4 of of IN cord-005207-02wmt2e9 32 5 nucleotides nucleotide NNS cord-005207-02wmt2e9 32 6 and and CC cord-005207-02wmt2e9 32 7 deduced deduce VBN cord-005207-02wmt2e9 32 8 amino amino JJ cord-005207-02wmt2e9 32 9 acids acid NNS cord-005207-02wmt2e9 32 10 of of IN cord-005207-02wmt2e9 32 11 the the DT cord-005207-02wmt2e9 32 12 N n CD cord-005207-02wmt2e9 32 13 gene gene NN cord-005207-02wmt2e9 32 14 were be VBD cord-005207-02wmt2e9 32 15 determined determine VBN cord-005207-02wmt2e9 32 16 , , , cord-005207-02wmt2e9 32 17 and and CC cord-005207-02wmt2e9 32 18 further further RB cord-005207-02wmt2e9 32 19 analyzed analyze VBN cord-005207-02wmt2e9 32 20 with with IN cord-005207-02wmt2e9 32 21 those those DT cord-005207-02wmt2e9 32 22 of of IN cord-005207-02wmt2e9 32 23 other other JJ cord-005207-02wmt2e9 32 24 PEDVs pedv NNS cord-005207-02wmt2e9 32 25 for for IN cord-005207-02wmt2e9 32 26 the the DT cord-005207-02wmt2e9 32 27 information information NN cord-005207-02wmt2e9 32 28 in in IN cord-005207-02wmt2e9 32 29 the the DT cord-005207-02wmt2e9 32 30 production production NN cord-005207-02wmt2e9 32 31 of of IN cord-005207-02wmt2e9 32 32 genetically genetically RB cord-005207-02wmt2e9 32 33 engineered engineer VBN cord-005207-02wmt2e9 32 34 diagnostic diagnostic JJ cord-005207-02wmt2e9 32 35 reagents reagent NNS cord-005207-02wmt2e9 32 36 . . . cord-005207-02wmt2e9 33 1 A a DT cord-005207-02wmt2e9 33 2 strain strain NN cord-005207-02wmt2e9 33 3 of of IN cord-005207-02wmt2e9 33 4 PEDV PEDV NNP cord-005207-02wmt2e9 33 5 , , , cord-005207-02wmt2e9 33 6 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 33 7 which which WDT cord-005207-02wmt2e9 33 8 was be VBD cord-005207-02wmt2e9 33 9 previously previously RB cord-005207-02wmt2e9 33 10 isolated isolate VBN cord-005207-02wmt2e9 33 11 from from IN cord-005207-02wmt2e9 33 12 the the DT cord-005207-02wmt2e9 33 13 intestinal intestinal JJ cord-005207-02wmt2e9 33 14 tissues tissue NNS cord-005207-02wmt2e9 33 15 of of IN cord-005207-02wmt2e9 33 16 piglets piglet NNS cord-005207-02wmt2e9 33 17 suffering suffer VBG cord-005207-02wmt2e9 33 18 from from IN cord-005207-02wmt2e9 33 19 severe severe JJ cord-005207-02wmt2e9 33 20 diarrhea diarrhea NN cord-005207-02wmt2e9 33 21 by by IN cord-005207-02wmt2e9 33 22 Virology Virology NNP cord-005207-02wmt2e9 33 23 Laboratory Laboratory NNP cord-005207-02wmt2e9 33 24 of of IN cord-005207-02wmt2e9 33 25 Gyeongsang Gyeongsang NNP cord-005207-02wmt2e9 33 26 National National NNP cord-005207-02wmt2e9 33 27 University University NNP cord-005207-02wmt2e9 33 28 College College NNP cord-005207-02wmt2e9 33 29 of of IN cord-005207-02wmt2e9 33 30 Veterinary Veterinary NNP cord-005207-02wmt2e9 33 31 Medicine Medicine NNP cord-005207-02wmt2e9 33 32 , , , cord-005207-02wmt2e9 33 33 Chinju Chinju NNP cord-005207-02wmt2e9 33 34 , , , cord-005207-02wmt2e9 33 35 Korea Korea NNP cord-005207-02wmt2e9 33 36 ( ( -LRB- cord-005207-02wmt2e9 33 37 data datum NNS cord-005207-02wmt2e9 33 38 not not RB cord-005207-02wmt2e9 33 39 shown show VBN cord-005207-02wmt2e9 33 40 ) ) -RRB- cord-005207-02wmt2e9 33 41 , , , cord-005207-02wmt2e9 33 42 was be VBD cord-005207-02wmt2e9 33 43 used use VBN cord-005207-02wmt2e9 33 44 . . . cord-005207-02wmt2e9 34 1 The the DT cord-005207-02wmt2e9 34 2 virus virus NN cord-005207-02wmt2e9 34 3 was be VBD cord-005207-02wmt2e9 34 4 propagated propagate VBN cord-005207-02wmt2e9 34 5 in in IN cord-005207-02wmt2e9 34 6 monolayer monolayer NN cord-005207-02wmt2e9 34 7 of of IN cord-005207-02wmt2e9 34 8 Vero Vero NNP cord-005207-02wmt2e9 34 9 cells cell NNS cord-005207-02wmt2e9 34 10 grown grow VBN cord-005207-02wmt2e9 34 11 in in IN cord-005207-02wmt2e9 34 12 minimal minimal JJ cord-005207-02wmt2e9 34 13 essential essential JJ cord-005207-02wmt2e9 34 14 medium medium NN cord-005207-02wmt2e9 34 15 ( ( -LRB- cord-005207-02wmt2e9 34 16 MEM mem NN cord-005207-02wmt2e9 34 17 ) ) -RRB- cord-005207-02wmt2e9 34 18 containing contain VBG cord-005207-02wmt2e9 34 19 streptomycin streptomycin NNS cord-005207-02wmt2e9 34 20 ( ( -LRB- cord-005207-02wmt2e9 34 21 100 100 CD cord-005207-02wmt2e9 34 22 mg mg NNP cord-005207-02wmt2e9 34 23 / / SYM cord-005207-02wmt2e9 34 24 ml ml NNP cord-005207-02wmt2e9 34 25 ) ) -RRB- cord-005207-02wmt2e9 34 26 , , , cord-005207-02wmt2e9 34 27 penicillin penicillin NN cord-005207-02wmt2e9 34 28 ( ( -LRB- cord-005207-02wmt2e9 34 29 100 100 CD cord-005207-02wmt2e9 34 30 U U NNP cord-005207-02wmt2e9 34 31 / / SYM cord-005207-02wmt2e9 34 32 ml ml NN cord-005207-02wmt2e9 34 33 ) ) -RRB- cord-005207-02wmt2e9 34 34 and and CC cord-005207-02wmt2e9 34 35 trypsin trypsin NN cord-005207-02wmt2e9 34 36 ( ( -LRB- cord-005207-02wmt2e9 34 37 10 10 CD cord-005207-02wmt2e9 34 38 mg mg NNP cord-005207-02wmt2e9 34 39 / / SYM cord-005207-02wmt2e9 34 40 ml ml NNP cord-005207-02wmt2e9 34 41 ) ) -RRB- cord-005207-02wmt2e9 34 42 in in IN cord-005207-02wmt2e9 34 43 a a DT cord-005207-02wmt2e9 34 44 5 5 CD cord-005207-02wmt2e9 34 45 % % NN cord-005207-02wmt2e9 34 46 CO co NN cord-005207-02wmt2e9 34 47 2 2 CD cord-005207-02wmt2e9 34 48 incubator incubator NN cord-005207-02wmt2e9 34 49 at at IN cord-005207-02wmt2e9 34 50 37 37 CD cord-005207-02wmt2e9 34 51 C c NN cord-005207-02wmt2e9 34 52 following follow VBG cord-005207-02wmt2e9 34 53 the the DT cord-005207-02wmt2e9 34 54 methods method NNS cord-005207-02wmt2e9 34 55 of of IN cord-005207-02wmt2e9 34 56 Hofmann Hofmann NNP cord-005207-02wmt2e9 34 57 and and CC cord-005207-02wmt2e9 34 58 Wyler Wyler NNP cord-005207-02wmt2e9 34 59 [ [ -LRB- cord-005207-02wmt2e9 34 60 13 13 CD cord-005207-02wmt2e9 34 61 ] ] -RRB- cord-005207-02wmt2e9 34 62 . . . cord-005207-02wmt2e9 35 1 When when WRB cord-005207-02wmt2e9 35 2 syncytial syncytial JJ cord-005207-02wmt2e9 35 3 formation formation NN cord-005207-02wmt2e9 35 4 appeared appear VBD cord-005207-02wmt2e9 35 5 in in IN cord-005207-02wmt2e9 35 6 the the DT cord-005207-02wmt2e9 35 7 Vero Vero NNP cord-005207-02wmt2e9 35 8 cells cell NNS cord-005207-02wmt2e9 35 9 after after IN cord-005207-02wmt2e9 35 10 propagation propagation NN cord-005207-02wmt2e9 35 11 of of IN cord-005207-02wmt2e9 35 12 the the DT cord-005207-02wmt2e9 35 13 virus virus NN cord-005207-02wmt2e9 35 14 , , , cord-005207-02wmt2e9 35 15 the the DT cord-005207-02wmt2e9 35 16 wasted waste VBN cord-005207-02wmt2e9 35 17 MEM MEM NNP cord-005207-02wmt2e9 35 18 was be VBD cord-005207-02wmt2e9 35 19 removed remove VBN cord-005207-02wmt2e9 35 20 . . . cord-005207-02wmt2e9 36 1 The the DT cord-005207-02wmt2e9 36 2 cells cell NNS cord-005207-02wmt2e9 36 3 were be VBD cord-005207-02wmt2e9 36 4 washed wash VBN cord-005207-02wmt2e9 36 5 with with IN cord-005207-02wmt2e9 36 6 PBS PBS NNP cord-005207-02wmt2e9 36 7 ( ( -LRB- cord-005207-02wmt2e9 36 8 pH pH NNP cord-005207-02wmt2e9 36 9 7.2 7.2 CD cord-005207-02wmt2e9 36 10 ) ) -RRB- cord-005207-02wmt2e9 36 11 and and CC cord-005207-02wmt2e9 36 12 lysed lyse VBN cord-005207-02wmt2e9 36 13 by by IN cord-005207-02wmt2e9 36 14 Trizol Trizol NNP cord-005207-02wmt2e9 36 15 1 1 CD cord-005207-02wmt2e9 36 16 reagent reagent NN cord-005207-02wmt2e9 36 17 ( ( -LRB- cord-005207-02wmt2e9 36 18 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 36 19 , , , cord-005207-02wmt2e9 36 20 USA USA NNP cord-005207-02wmt2e9 36 21 ) ) -RRB- cord-005207-02wmt2e9 36 22 at at IN cord-005207-02wmt2e9 36 23 2 2 CD cord-005207-02wmt2e9 36 24 ml ml NN cord-005207-02wmt2e9 36 25 per per IN cord-005207-02wmt2e9 36 26 tissue tissue NN cord-005207-02wmt2e9 36 27 culture culture NN cord-005207-02wmt2e9 36 28 flask flask NN cord-005207-02wmt2e9 36 29 ( ( -LRB- cord-005207-02wmt2e9 36 30 25 25 CD cord-005207-02wmt2e9 36 31 cm cm NNS cord-005207-02wmt2e9 36 32 2 2 CD cord-005207-02wmt2e9 36 33 ) ) -RRB- cord-005207-02wmt2e9 36 34 , , , cord-005207-02wmt2e9 36 35 and and CC cord-005207-02wmt2e9 36 36 homogenized homogenize VBN cord-005207-02wmt2e9 36 37 by by IN cord-005207-02wmt2e9 36 38 passing pass VBG cord-005207-02wmt2e9 36 39 the the DT cord-005207-02wmt2e9 36 40 cell cell NN cord-005207-02wmt2e9 36 41 lysate lysate NN cord-005207-02wmt2e9 36 42 several several JJ cord-005207-02wmt2e9 36 43 times time NNS cord-005207-02wmt2e9 36 44 through through IN cord-005207-02wmt2e9 36 45 a a DT cord-005207-02wmt2e9 36 46 pipette pipette NN cord-005207-02wmt2e9 36 47 . . . cord-005207-02wmt2e9 37 1 Viral Viral NNP cord-005207-02wmt2e9 37 2 RNA RNA NNP cord-005207-02wmt2e9 37 3 was be VBD cord-005207-02wmt2e9 37 4 extracted extract VBN cord-005207-02wmt2e9 37 5 from from IN cord-005207-02wmt2e9 37 6 the the DT cord-005207-02wmt2e9 37 7 homogenate homogenate NNP cord-005207-02wmt2e9 37 8 following follow VBG cord-005207-02wmt2e9 37 9 the the DT cord-005207-02wmt2e9 37 10 manufacturer manufacturer NN cord-005207-02wmt2e9 37 11 's 's POS cord-005207-02wmt2e9 37 12 suggestions suggestion NNS cord-005207-02wmt2e9 37 13 and and CC cord-005207-02wmt2e9 37 14 dissolved dissolve VBD cord-005207-02wmt2e9 37 15 in in IN cord-005207-02wmt2e9 37 16 diethyl diethyl NNP cord-005207-02wmt2e9 37 17 pyrocarbonate pyrocarbonate NNP cord-005207-02wmt2e9 37 18 - - HYPH cord-005207-02wmt2e9 37 19 treated treat VBN cord-005207-02wmt2e9 37 20 distilled distilled JJ cord-005207-02wmt2e9 37 21 water water NN cord-005207-02wmt2e9 37 22 . . . cord-005207-02wmt2e9 38 1 A a DT cord-005207-02wmt2e9 38 2 pair pair NN cord-005207-02wmt2e9 38 3 of of IN cord-005207-02wmt2e9 38 4 sense sense NN cord-005207-02wmt2e9 38 5 and and CC cord-005207-02wmt2e9 38 6 antisense antisense NN cord-005207-02wmt2e9 38 7 primers primer NNS cord-005207-02wmt2e9 38 8 was be VBD cord-005207-02wmt2e9 38 9 designed design VBN cord-005207-02wmt2e9 38 10 and and CC cord-005207-02wmt2e9 38 11 aligned align VBN cord-005207-02wmt2e9 38 12 based base VBN cord-005207-02wmt2e9 38 13 on on IN cord-005207-02wmt2e9 38 14 nucleotide nucleotide JJ cord-005207-02wmt2e9 38 15 sequences sequence NNS cord-005207-02wmt2e9 38 16 of of IN cord-005207-02wmt2e9 38 17 the the DT cord-005207-02wmt2e9 38 18 N n CD cord-005207-02wmt2e9 38 19 gene gene NN cord-005207-02wmt2e9 38 20 of of IN cord-005207-02wmt2e9 38 21 CV777 CV777 NNP cord-005207-02wmt2e9 39 1 and and CC cord-005207-02wmt2e9 39 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 39 3 [ [ -LRB- cord-005207-02wmt2e9 39 4 10 10 CD cord-005207-02wmt2e9 39 5 , , , cord-005207-02wmt2e9 39 6 14 14 CD cord-005207-02wmt2e9 39 7 ] ] -RRB- cord-005207-02wmt2e9 39 8 from from IN cord-005207-02wmt2e9 39 9 the the DT cord-005207-02wmt2e9 39 10 GenBank GenBank NNP cord-005207-02wmt2e9 39 11 data data NN cord-005207-02wmt2e9 39 12 base base NN cord-005207-02wmt2e9 39 13 ( ( -LRB- cord-005207-02wmt2e9 39 14 National National NNP cord-005207-02wmt2e9 39 15 Center Center NNP cord-005207-02wmt2e9 39 16 for for IN cord-005207-02wmt2e9 39 17 Biotechnology Biotechnology NNP cord-005207-02wmt2e9 39 18 Information Information NNP cord-005207-02wmt2e9 39 19 , , , cord-005207-02wmt2e9 39 20 USA USA NNP cord-005207-02wmt2e9 39 21 ) ) -RRB- cord-005207-02wmt2e9 39 22 . . . cord-005207-02wmt2e9 40 1 The the DT cord-005207-02wmt2e9 40 2 sense sense NN cord-005207-02wmt2e9 40 3 primer primer NN cord-005207-02wmt2e9 40 4 NF1 NF1 NNP cord-005207-02wmt2e9 40 5 ( ( -LRB- cord-005207-02wmt2e9 40 6 5 5 CD cord-005207-02wmt2e9 40 7 0 0 CD cord-005207-02wmt2e9 40 8 CCGAGTGC CCGAGTGC NNP cord-005207-02wmt2e9 40 9 - - HYPH cord-005207-02wmt2e9 40 10 GGTTCTCACAGAT3 GGTTCTCACAGAT3 NNP cord-005207-02wmt2e9 40 11 0 0 CD cord-005207-02wmt2e9 40 12 ) ) -RRB- cord-005207-02wmt2e9 40 13 and and CC cord-005207-02wmt2e9 40 14 antisense antisense NN cord-005207-02wmt2e9 40 15 primer primer NN cord-005207-02wmt2e9 40 16 NR1 NR1 NNP cord-005207-02wmt2e9 40 17 ( ( -LRB- cord-005207-02wmt2e9 40 18 5 5 CD cord-005207-02wmt2e9 40 19 0 0 CD cord-005207-02wmt2e9 40 20 CATAGCCAGGATAAGCCGGTC3 catagccaggataagccggtc3 NN cord-005207-02wmt2e9 40 21 0 0 CD cord-005207-02wmt2e9 40 22 ) ) -RRB- cord-005207-02wmt2e9 40 23 were be VBD cord-005207-02wmt2e9 40 24 used use VBN cord-005207-02wmt2e9 40 25 to to TO cord-005207-02wmt2e9 40 26 generate generate VB cord-005207-02wmt2e9 40 27 cDNA cdna NN cord-005207-02wmt2e9 40 28 for for IN cord-005207-02wmt2e9 40 29 the the DT cord-005207-02wmt2e9 40 30 N n CD cord-005207-02wmt2e9 40 31 gene gene NN cord-005207-02wmt2e9 40 32 of of IN cord-005207-02wmt2e9 40 33 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 40 34 and and CC cord-005207-02wmt2e9 40 35 relative relative JJ cord-005207-02wmt2e9 40 36 position position NN cord-005207-02wmt2e9 40 37 of of IN cord-005207-02wmt2e9 40 38 the the DT cord-005207-02wmt2e9 40 39 primers primer NNS cord-005207-02wmt2e9 40 40 are be VBP cord-005207-02wmt2e9 40 41 shown show VBN cord-005207-02wmt2e9 40 42 in in IN cord-005207-02wmt2e9 40 43 Fig Fig NNP cord-005207-02wmt2e9 40 44 . . . cord-005207-02wmt2e9 41 1 2 2 LS cord-005207-02wmt2e9 41 2 . . . cord-005207-02wmt2e9 42 1 Synthesis synthesis NN cord-005207-02wmt2e9 42 2 of of IN cord-005207-02wmt2e9 42 3 the the DT cord-005207-02wmt2e9 42 4 first first JJ cord-005207-02wmt2e9 42 5 - - HYPH cord-005207-02wmt2e9 42 6 strand strand NN cord-005207-02wmt2e9 42 7 cDNA cdna NN cord-005207-02wmt2e9 42 8 for for IN cord-005207-02wmt2e9 42 9 the the DT cord-005207-02wmt2e9 42 10 N N NNP cord-005207-02wmt2e9 42 11 gene gene NN cord-005207-02wmt2e9 42 12 was be VBD cord-005207-02wmt2e9 42 13 carried carry VBN cord-005207-02wmt2e9 42 14 out out RP cord-005207-02wmt2e9 42 15 by by IN cord-005207-02wmt2e9 42 16 reverse reverse JJ cord-005207-02wmt2e9 42 17 transcription transcription NN cord-005207-02wmt2e9 42 18 ( ( -LRB- cord-005207-02wmt2e9 42 19 RT RT NNP cord-005207-02wmt2e9 42 20 ) ) -RRB- cord-005207-02wmt2e9 42 21 using use VBG cord-005207-02wmt2e9 42 22 Superscript Superscript NNP cord-005207-02wmt2e9 42 23 II II NNP cord-005207-02wmt2e9 42 24 1 1 CD cord-005207-02wmt2e9 42 25 reverse reverse NN cord-005207-02wmt2e9 42 26 transcriptase transcriptase NN cord-005207-02wmt2e9 42 27 reagent reagent NN cord-005207-02wmt2e9 42 28 kit kit NN cord-005207-02wmt2e9 42 29 ( ( -LRB- cord-005207-02wmt2e9 42 30 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 42 31 ) ) -RRB- cord-005207-02wmt2e9 42 32 following follow VBG cord-005207-02wmt2e9 42 33 manufacturer manufacturer NN cord-005207-02wmt2e9 42 34 's 's POS cord-005207-02wmt2e9 42 35 suggestions suggestion NNS cord-005207-02wmt2e9 42 36 . . . cord-005207-02wmt2e9 43 1 The the DT cord-005207-02wmt2e9 43 2 viral viral JJ cord-005207-02wmt2e9 43 3 RNA RNA NNP cord-005207-02wmt2e9 43 4 was be VBD cord-005207-02wmt2e9 43 5 mixed mix VBN cord-005207-02wmt2e9 43 6 with with IN cord-005207-02wmt2e9 43 7 1 1 CD cord-005207-02wmt2e9 43 8 ml ml NNS cord-005207-02wmt2e9 43 9 of of IN cord-005207-02wmt2e9 43 10 100 100 CD cord-005207-02wmt2e9 43 11 pM pm NN cord-005207-02wmt2e9 43 12 of of IN cord-005207-02wmt2e9 43 13 the the DT cord-005207-02wmt2e9 43 14 antisense antisense NN cord-005207-02wmt2e9 43 15 primer primer NN cord-005207-02wmt2e9 43 16 , , , cord-005207-02wmt2e9 43 17 4 4 CD cord-005207-02wmt2e9 43 18 ml ml NNS cord-005207-02wmt2e9 43 19 of of IN cord-005207-02wmt2e9 43 20 5X 5x CD cord-005207-02wmt2e9 43 21 first first JJ cord-005207-02wmt2e9 43 22 - - HYPH cord-005207-02wmt2e9 43 23 strand strand NN cord-005207-02wmt2e9 43 24 buffer buffer NN cord-005207-02wmt2e9 43 25 , , , cord-005207-02wmt2e9 43 26 1 1 CD cord-005207-02wmt2e9 43 27 ml ml NNS cord-005207-02wmt2e9 43 28 of of IN cord-005207-02wmt2e9 43 29 10 10 CD cord-005207-02wmt2e9 43 30 mM mm NN cord-005207-02wmt2e9 43 31 dNTP dntp NN cord-005207-02wmt2e9 43 32 mixture mixture NN cord-005207-02wmt2e9 43 33 , , , cord-005207-02wmt2e9 43 34 2 2 CD cord-005207-02wmt2e9 43 35 ml ml NN cord-005207-02wmt2e9 43 36 of of IN cord-005207-02wmt2e9 43 37 0.1 0.1 CD cord-005207-02wmt2e9 43 38 M M NNP cord-005207-02wmt2e9 43 39 DTT DTT NNP cord-005207-02wmt2e9 43 40 , , , cord-005207-02wmt2e9 43 41 1 1 CD cord-005207-02wmt2e9 43 42 ml ml NNS cord-005207-02wmt2e9 43 43 of of IN cord-005207-02wmt2e9 43 44 RNase RNase NNP cord-005207-02wmt2e9 43 45 inhibitor inhibitor NN cord-005207-02wmt2e9 43 46 ( ( -LRB- cord-005207-02wmt2e9 43 47 40 40 CD cord-005207-02wmt2e9 43 48 U U NNP cord-005207-02wmt2e9 43 49 / / SYM cord-005207-02wmt2e9 43 50 ml ml NN cord-005207-02wmt2e9 43 51 ) ) -RRB- cord-005207-02wmt2e9 43 52 , , , cord-005207-02wmt2e9 43 53 1 1 CD cord-005207-02wmt2e9 43 54 ml ml NNS cord-005207-02wmt2e9 43 55 of of IN cord-005207-02wmt2e9 43 56 reverse reverse JJ cord-005207-02wmt2e9 43 57 transcriptase transcriptase NN cord-005207-02wmt2e9 43 58 ( ( -LRB- cord-005207-02wmt2e9 43 59 200 200 CD cord-005207-02wmt2e9 43 60 U u NN cord-005207-02wmt2e9 43 61 / / SYM cord-005207-02wmt2e9 43 62 ml ml NN cord-005207-02wmt2e9 43 63 ) ) -RRB- cord-005207-02wmt2e9 43 64 and and CC cord-005207-02wmt2e9 43 65 brought bring VBD cord-005207-02wmt2e9 43 66 to to IN cord-005207-02wmt2e9 43 67 20 20 CD cord-005207-02wmt2e9 43 68 ml ml NNS cord-005207-02wmt2e9 43 69 with with IN cord-005207-02wmt2e9 43 70 distilled distilled JJ cord-005207-02wmt2e9 43 71 water water NN cord-005207-02wmt2e9 43 72 . . . cord-005207-02wmt2e9 44 1 The the DT cord-005207-02wmt2e9 44 2 reaction reaction NN cord-005207-02wmt2e9 44 3 mixture mixture NN cord-005207-02wmt2e9 44 4 was be VBD cord-005207-02wmt2e9 44 5 incubated incubate VBN cord-005207-02wmt2e9 44 6 for for IN cord-005207-02wmt2e9 44 7 50 50 CD cord-005207-02wmt2e9 44 8 min min NN cord-005207-02wmt2e9 44 9 at at IN cord-005207-02wmt2e9 44 10 42 42 CD cord-005207-02wmt2e9 44 11 C C NNP cord-005207-02wmt2e9 44 12 , , , cord-005207-02wmt2e9 44 13 and and CC cord-005207-02wmt2e9 44 14 the the DT cord-005207-02wmt2e9 44 15 reaction reaction NN cord-005207-02wmt2e9 44 16 was be VBD cord-005207-02wmt2e9 44 17 stopped stop VBN cord-005207-02wmt2e9 44 18 by by IN cord-005207-02wmt2e9 44 19 heat heat NN cord-005207-02wmt2e9 44 20 for for IN cord-005207-02wmt2e9 44 21 15 15 CD cord-005207-02wmt2e9 44 22 min min NN cord-005207-02wmt2e9 44 23 at at IN cord-005207-02wmt2e9 44 24 70 70 CD cord-005207-02wmt2e9 44 25 C. c. NN cord-005207-02wmt2e9 45 1 To to TO cord-005207-02wmt2e9 45 2 degrade degrade VB cord-005207-02wmt2e9 45 3 RNA RNA NNP cord-005207-02wmt2e9 45 4 template template NN cord-005207-02wmt2e9 45 5 , , , cord-005207-02wmt2e9 45 6 the the DT cord-005207-02wmt2e9 45 7 reaction reaction NN cord-005207-02wmt2e9 45 8 mixture mixture NN cord-005207-02wmt2e9 45 9 was be VBD cord-005207-02wmt2e9 45 10 treated treat VBN cord-005207-02wmt2e9 45 11 with with IN cord-005207-02wmt2e9 45 12 RNase RNase NNP cord-005207-02wmt2e9 45 13 H h NN cord-005207-02wmt2e9 45 14 ( ( -LRB- cord-005207-02wmt2e9 45 15 1 1 CD cord-005207-02wmt2e9 45 16 U u NN cord-005207-02wmt2e9 45 17 ) ) -RRB- cord-005207-02wmt2e9 45 18 for for IN cord-005207-02wmt2e9 45 19 20 20 CD cord-005207-02wmt2e9 45 20 min min NN cord-005207-02wmt2e9 45 21 at at IN cord-005207-02wmt2e9 45 22 37 37 CD cord-005207-02wmt2e9 45 23 C. c. NN cord-005207-02wmt2e9 46 1 The the DT cord-005207-02wmt2e9 46 2 ds ds NNP cord-005207-02wmt2e9 46 3 - - HYPH cord-005207-02wmt2e9 46 4 cDNA cDNA NNP cord-005207-02wmt2e9 46 5 for for IN cord-005207-02wmt2e9 46 6 the the DT cord-005207-02wmt2e9 46 7 N N NNP cord-005207-02wmt2e9 46 8 gene gene NN cord-005207-02wmt2e9 46 9 was be VBD cord-005207-02wmt2e9 46 10 synthesized synthesize VBN cord-005207-02wmt2e9 46 11 by by IN cord-005207-02wmt2e9 46 12 polymerase polymerase NN cord-005207-02wmt2e9 46 13 chain chain NN cord-005207-02wmt2e9 46 14 reaction reaction NN cord-005207-02wmt2e9 46 15 ( ( -LRB- cord-005207-02wmt2e9 46 16 PCR PCR NNP cord-005207-02wmt2e9 46 17 ) ) -RRB- cord-005207-02wmt2e9 46 18 using use VBG cord-005207-02wmt2e9 46 19 a a DT cord-005207-02wmt2e9 46 20 reagent reagent JJ cord-005207-02wmt2e9 46 21 kit kit NN cord-005207-02wmt2e9 46 22 ( ( -LRB- cord-005207-02wmt2e9 46 23 Perkin Perkin NNP cord-005207-02wmt2e9 46 24 - - HYPH cord-005207-02wmt2e9 46 25 Elmer Elmer NNP cord-005207-02wmt2e9 46 26 , , , cord-005207-02wmt2e9 46 27 USA USA NNP cord-005207-02wmt2e9 46 28 ) ) -RRB- cord-005207-02wmt2e9 46 29 . . . cord-005207-02wmt2e9 47 1 A a DT cord-005207-02wmt2e9 47 2 10 10 CD cord-005207-02wmt2e9 47 3 ml ml NN cord-005207-02wmt2e9 47 4 portion portion NN cord-005207-02wmt2e9 47 5 of of IN cord-005207-02wmt2e9 47 6 the the DT cord-005207-02wmt2e9 47 7 firststrand firststrand NNP cord-005207-02wmt2e9 47 8 cDNA cdna NN cord-005207-02wmt2e9 47 9 template template NN cord-005207-02wmt2e9 47 10 was be VBD cord-005207-02wmt2e9 47 11 added add VBN cord-005207-02wmt2e9 47 12 to to IN cord-005207-02wmt2e9 47 13 5 5 CD cord-005207-02wmt2e9 47 14 ml ml NNS cord-005207-02wmt2e9 47 15 of of IN cord-005207-02wmt2e9 47 16 10X 10x CD cord-005207-02wmt2e9 47 17 PCR PCR NNP cord-005207-02wmt2e9 47 18 buffer buffer NN cord-005207-02wmt2e9 47 19 , , , cord-005207-02wmt2e9 47 20 4 4 CD cord-005207-02wmt2e9 47 21 ml ml NNS cord-005207-02wmt2e9 47 22 of of IN cord-005207-02wmt2e9 47 23 25 25 CD cord-005207-02wmt2e9 47 24 mM mm CD cord-005207-02wmt2e9 47 25 MgCl MgCl NNP cord-005207-02wmt2e9 47 26 2 2 CD cord-005207-02wmt2e9 47 27 , , , cord-005207-02wmt2e9 47 28 1 1 CD cord-005207-02wmt2e9 47 29 ml ml NNS cord-005207-02wmt2e9 47 30 of of IN cord-005207-02wmt2e9 47 31 10 10 CD cord-005207-02wmt2e9 47 32 mM mm NN cord-005207-02wmt2e9 47 33 dNTP dntp NN cord-005207-02wmt2e9 47 34 mixture mixture NN cord-005207-02wmt2e9 47 35 , , , cord-005207-02wmt2e9 47 36 1 1 CD cord-005207-02wmt2e9 47 37 ml ml NNS cord-005207-02wmt2e9 47 38 of of IN cord-005207-02wmt2e9 47 39 each each DT cord-005207-02wmt2e9 47 40 100 100 CD cord-005207-02wmt2e9 47 41 pM pm NN cord-005207-02wmt2e9 47 42 sense sense NN cord-005207-02wmt2e9 47 43 and and CC cord-005207-02wmt2e9 47 44 antisense antisense NN cord-005207-02wmt2e9 47 45 primers primer NNS cord-005207-02wmt2e9 47 46 , , , cord-005207-02wmt2e9 47 47 1 1 CD cord-005207-02wmt2e9 47 48 ml ml NNS cord-005207-02wmt2e9 47 49 of of IN cord-005207-02wmt2e9 47 50 Taq Taq NNP cord-005207-02wmt2e9 47 51 DNA dna NN cord-005207-02wmt2e9 47 52 polymerase polymerase NN cord-005207-02wmt2e9 47 53 ( ( -LRB- cord-005207-02wmt2e9 47 54 5 5 CD cord-005207-02wmt2e9 47 55 U u NN cord-005207-02wmt2e9 47 56 / / SYM cord-005207-02wmt2e9 47 57 ml ml NN cord-005207-02wmt2e9 47 58 ) ) -RRB- cord-005207-02wmt2e9 47 59 and and CC cord-005207-02wmt2e9 47 60 brought bring VBN cord-005207-02wmt2e9 47 61 to to IN cord-005207-02wmt2e9 47 62 50 50 CD cord-005207-02wmt2e9 47 63 ml ml NNS cord-005207-02wmt2e9 47 64 with with IN cord-005207-02wmt2e9 47 65 distilled distilled JJ cord-005207-02wmt2e9 47 66 water water NN cord-005207-02wmt2e9 47 67 . . . cord-005207-02wmt2e9 48 1 The the DT cord-005207-02wmt2e9 48 2 PCR PCR NNP cord-005207-02wmt2e9 48 3 was be VBD cord-005207-02wmt2e9 48 4 carried carry VBN cord-005207-02wmt2e9 48 5 out out RP cord-005207-02wmt2e9 48 6 in in IN cord-005207-02wmt2e9 48 7 a a DT cord-005207-02wmt2e9 48 8 thermocycler thermocycler NN cord-005207-02wmt2e9 48 9 ( ( -LRB- cord-005207-02wmt2e9 48 10 Perkin Perkin NNP cord-005207-02wmt2e9 48 11 - - HYPH cord-005207-02wmt2e9 48 12 Elmer Elmer NNP cord-005207-02wmt2e9 48 13 ) ) -RRB- cord-005207-02wmt2e9 48 14 following follow VBG cord-005207-02wmt2e9 48 15 the the DT cord-005207-02wmt2e9 48 16 program program NN cord-005207-02wmt2e9 48 17 of of IN cord-005207-02wmt2e9 48 18 2 2 CD cord-005207-02wmt2e9 48 19 min min NN cord-005207-02wmt2e9 48 20 at at IN cord-005207-02wmt2e9 48 21 94 94 CD cord-005207-02wmt2e9 48 22 C c NN cord-005207-02wmt2e9 48 23 and and CC cord-005207-02wmt2e9 48 24 30 30 CD cord-005207-02wmt2e9 48 25 cycles cycle NNS cord-005207-02wmt2e9 48 26 of of IN cord-005207-02wmt2e9 48 27 1 1 CD cord-005207-02wmt2e9 48 28 min min NN cord-005207-02wmt2e9 48 29 at at IN cord-005207-02wmt2e9 48 30 94 94 CD cord-005207-02wmt2e9 48 31 C C NNP cord-005207-02wmt2e9 48 32 , , , cord-005207-02wmt2e9 48 33 1 1 CD cord-005207-02wmt2e9 48 34 min min NN cord-005207-02wmt2e9 48 35 at at IN cord-005207-02wmt2e9 48 36 55 55 CD cord-005207-02wmt2e9 48 37 C c NN cord-005207-02wmt2e9 48 38 and and CC cord-005207-02wmt2e9 48 39 1 1 CD cord-005207-02wmt2e9 48 40 min min NN cord-005207-02wmt2e9 48 41 at at IN cord-005207-02wmt2e9 48 42 72 72 CD cord-005207-02wmt2e9 48 43 C c NN cord-005207-02wmt2e9 48 44 , , , cord-005207-02wmt2e9 48 45 and and CC cord-005207-02wmt2e9 48 46 a a DT cord-005207-02wmt2e9 48 47 final final JJ cord-005207-02wmt2e9 48 48 extension extension NN cord-005207-02wmt2e9 48 49 at at IN cord-005207-02wmt2e9 48 50 72 72 CD cord-005207-02wmt2e9 48 51 C c NN cord-005207-02wmt2e9 48 52 for for IN cord-005207-02wmt2e9 48 53 5 5 CD cord-005207-02wmt2e9 48 54 min min NN cord-005207-02wmt2e9 48 55 . . . cord-005207-02wmt2e9 49 1 The the DT cord-005207-02wmt2e9 49 2 PCR PCR NNP cord-005207-02wmt2e9 49 3 products product NNS cord-005207-02wmt2e9 49 4 were be VBD cord-005207-02wmt2e9 49 5 resolved resolve VBN cord-005207-02wmt2e9 49 6 by by IN cord-005207-02wmt2e9 49 7 electrophoresis electrophoresis NN cord-005207-02wmt2e9 49 8 in in IN cord-005207-02wmt2e9 49 9 1 1 CD cord-005207-02wmt2e9 49 10 % % NN cord-005207-02wmt2e9 49 11 agarose agarose NN cord-005207-02wmt2e9 49 12 gel gel NN cord-005207-02wmt2e9 49 13 . . . cord-005207-02wmt2e9 50 1 Following follow VBG cord-005207-02wmt2e9 50 2 the the DT cord-005207-02wmt2e9 50 3 routine routine JJ cord-005207-02wmt2e9 50 4 methods method NNS cord-005207-02wmt2e9 50 5 in in IN cord-005207-02wmt2e9 50 6 gene gene NN cord-005207-02wmt2e9 50 7 cloning clone VBG cord-005207-02wmt2e9 50 8 [ [ -LRB- cord-005207-02wmt2e9 50 9 15 15 CD cord-005207-02wmt2e9 50 10 ] ] -RRB- cord-005207-02wmt2e9 50 11 , , , cord-005207-02wmt2e9 50 12 the the DT cord-005207-02wmt2e9 50 13 PCR PCR NNP cord-005207-02wmt2e9 50 14 - - HYPH cord-005207-02wmt2e9 50 15 generated generate VBN cord-005207-02wmt2e9 50 16 N n CD cord-005207-02wmt2e9 50 17 gene gene NN cord-005207-02wmt2e9 50 18 ds ds NNP cord-005207-02wmt2e9 50 19 - - HYPH cord-005207-02wmt2e9 50 20 cDNAs cDNAs NNPS cord-005207-02wmt2e9 50 21 were be VBD cord-005207-02wmt2e9 50 22 bluntended bluntende VBN cord-005207-02wmt2e9 50 23 with with IN cord-005207-02wmt2e9 50 24 Klenow Klenow NNP cord-005207-02wmt2e9 50 25 enzyme enzyme NN cord-005207-02wmt2e9 50 26 ( ( -LRB- cord-005207-02wmt2e9 50 27 2 2 CD cord-005207-02wmt2e9 50 28 U U NNP cord-005207-02wmt2e9 50 29 ) ) -RRB- cord-005207-02wmt2e9 50 30 and and CC cord-005207-02wmt2e9 50 31 1 1 CD cord-005207-02wmt2e9 50 32 ml ml NNS cord-005207-02wmt2e9 50 33 of of IN cord-005207-02wmt2e9 51 1 0.5 0.5 CD cord-005207-02wmt2e9 51 2 mM mm CD cord-005207-02wmt2e9 51 3 dNTPs dntps CD cord-005207-02wmt2e9 51 4 ( ( -LRB- cord-005207-02wmt2e9 51 5 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 51 6 ) ) -RRB- cord-005207-02wmt2e9 51 7 in in IN cord-005207-02wmt2e9 51 8 20 20 CD cord-005207-02wmt2e9 51 9 ml ml NN cord-005207-02wmt2e9 51 10 reaction reaction NN cord-005207-02wmt2e9 51 11 volume volume NN cord-005207-02wmt2e9 51 12 and and CC cord-005207-02wmt2e9 51 13 cloned clone VBN cord-005207-02wmt2e9 51 14 into into IN cord-005207-02wmt2e9 51 15 the the DT cord-005207-02wmt2e9 51 16 SmaI SmaI NNP cord-005207-02wmt2e9 51 17 site site NN cord-005207-02wmt2e9 51 18 of of IN cord-005207-02wmt2e9 51 19 pTZ19R ptz19r NN cord-005207-02wmt2e9 51 20 plasmid plasmid NN cord-005207-02wmt2e9 51 21 DNA dna NN cord-005207-02wmt2e9 51 22 by by IN cord-005207-02wmt2e9 51 23 ligation ligation NN cord-005207-02wmt2e9 51 24 using use VBG cord-005207-02wmt2e9 51 25 T4 T4 NNP cord-005207-02wmt2e9 51 26 DNA DNA NNP cord-005207-02wmt2e9 51 27 ligase ligase NN cord-005207-02wmt2e9 51 28 ( ( -LRB- cord-005207-02wmt2e9 51 29 1 1 CD cord-005207-02wmt2e9 51 30 U u NN cord-005207-02wmt2e9 51 31 ) ) -RRB- cord-005207-02wmt2e9 51 32 ( ( -LRB- cord-005207-02wmt2e9 51 33 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 51 34 ) ) -RRB- cord-005207-02wmt2e9 51 35 . . . cord-005207-02wmt2e9 52 1 The the DT cord-005207-02wmt2e9 52 2 recombinant recombinant JJ cord-005207-02wmt2e9 52 3 plasmid plasmid NN cord-005207-02wmt2e9 52 4 DNAs dna NNS cord-005207-02wmt2e9 52 5 were be VBD cord-005207-02wmt2e9 52 6 transformed transform VBN cord-005207-02wmt2e9 52 7 into into IN cord-005207-02wmt2e9 52 8 competent competent JJ cord-005207-02wmt2e9 52 9 Escherichia Escherichia NNP cord-005207-02wmt2e9 52 10 coli coli NNS cord-005207-02wmt2e9 52 11 DH5a DH5a NNP cord-005207-02wmt2e9 52 12 cells cell NNS cord-005207-02wmt2e9 52 13 by by IN cord-005207-02wmt2e9 52 14 heat heat NN cord-005207-02wmt2e9 52 15 shock shock NN cord-005207-02wmt2e9 52 16 for for IN cord-005207-02wmt2e9 52 17 45 45 CD cord-005207-02wmt2e9 52 18 s s NN cord-005207-02wmt2e9 52 19 at at IN cord-005207-02wmt2e9 52 20 42 42 CD cord-005207-02wmt2e9 52 21 C. C. NNP cord-005207-02wmt2e9 52 22 After after IN cord-005207-02wmt2e9 52 23 adding add VBG cord-005207-02wmt2e9 52 24 SOC SOC NNP cord-005207-02wmt2e9 52 25 medium medium NN cord-005207-02wmt2e9 52 26 ( ( -LRB- cord-005207-02wmt2e9 52 27 0.5 0.5 CD cord-005207-02wmt2e9 52 28 % % NN cord-005207-02wmt2e9 52 29 yeast yeast NN cord-005207-02wmt2e9 52 30 extract extract NN cord-005207-02wmt2e9 52 31 , , , cord-005207-02wmt2e9 52 32 2 2 CD cord-005207-02wmt2e9 52 33 % % NN cord-005207-02wmt2e9 52 34 tryptone tryptone NN cord-005207-02wmt2e9 52 35 , , , cord-005207-02wmt2e9 52 36 10 10 CD cord-005207-02wmt2e9 52 37 mM mm CD cord-005207-02wmt2e9 52 38 NaCl nacl NN cord-005207-02wmt2e9 52 39 , , , cord-005207-02wmt2e9 52 40 2.5 2.5 CD cord-005207-02wmt2e9 52 41 mM mm CD cord-005207-02wmt2e9 52 42 KCl KCl NNS cord-005207-02wmt2e9 52 43 , , , cord-005207-02wmt2e9 52 44 10 10 CD cord-005207-02wmt2e9 52 45 mM mm CD cord-005207-02wmt2e9 52 46 MgCl MgCl NNP cord-005207-02wmt2e9 52 47 2 2 CD cord-005207-02wmt2e9 52 48 , , , cord-005207-02wmt2e9 53 1 20 20 CD cord-005207-02wmt2e9 53 2 mM mm CD cord-005207-02wmt2e9 53 3 MgSO MgSO NNP cord-005207-02wmt2e9 53 4 4 4 CD cord-005207-02wmt2e9 53 5 , , , cord-005207-02wmt2e9 53 6 20 20 CD cord-005207-02wmt2e9 53 7 mM mm CD cord-005207-02wmt2e9 53 8 glucose glucose NN cord-005207-02wmt2e9 53 9 ) ) -RRB- cord-005207-02wmt2e9 53 10 , , , cord-005207-02wmt2e9 53 11 the the DT cord-005207-02wmt2e9 53 12 tube tube NN cord-005207-02wmt2e9 53 13 was be VBD cord-005207-02wmt2e9 53 14 shaken shake VBN cord-005207-02wmt2e9 53 15 for for IN cord-005207-02wmt2e9 53 16 1 1 CD cord-005207-02wmt2e9 53 17 h h NN cord-005207-02wmt2e9 53 18 at at IN cord-005207-02wmt2e9 53 19 220 220 CD cord-005207-02wmt2e9 53 20 rpm rpm NN cord-005207-02wmt2e9 53 21 , , , cord-005207-02wmt2e9 53 22 37 37 CD cord-005207-02wmt2e9 53 23 C. c. NN cord-005207-02wmt2e9 54 1 The the DT cord-005207-02wmt2e9 54 2 transformed transformed JJ cord-005207-02wmt2e9 54 3 cells cell NNS cord-005207-02wmt2e9 54 4 were be VBD cord-005207-02wmt2e9 54 5 plated plate VBN cord-005207-02wmt2e9 54 6 onto onto IN cord-005207-02wmt2e9 54 7 Luria Luria NNP cord-005207-02wmt2e9 54 8 Bertani Bertani NNP cord-005207-02wmt2e9 54 9 ( ( -LRB- cord-005207-02wmt2e9 54 10 LB LB NNP cord-005207-02wmt2e9 54 11 ) ) -RRB- cord-005207-02wmt2e9 54 12 agar agar NN cord-005207-02wmt2e9 54 13 ( ( -LRB- cord-005207-02wmt2e9 54 14 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 54 15 ) ) -RRB- cord-005207-02wmt2e9 54 16 containing contain VBG cord-005207-02wmt2e9 54 17 ampicillin ampicillin NN cord-005207-02wmt2e9 54 18 ( ( -LRB- cord-005207-02wmt2e9 54 19 50 50 CD cord-005207-02wmt2e9 54 20 mg mg NNP cord-005207-02wmt2e9 54 21 / / SYM cord-005207-02wmt2e9 54 22 ml ml NNP cord-005207-02wmt2e9 54 23 ) ) -RRB- cord-005207-02wmt2e9 54 24 , , , cord-005207-02wmt2e9 54 25 X x NN cord-005207-02wmt2e9 54 26 - - NN cord-005207-02wmt2e9 54 27 gal gal NN cord-005207-02wmt2e9 54 28 ( ( -LRB- cord-005207-02wmt2e9 54 29 40 40 CD cord-005207-02wmt2e9 54 30 mg mg NNP cord-005207-02wmt2e9 54 31 / / SYM cord-005207-02wmt2e9 54 32 ml ml NNP cord-005207-02wmt2e9 54 33 ) ) -RRB- cord-005207-02wmt2e9 54 34 and and CC cord-005207-02wmt2e9 54 35 isopropylthio isopropylthio NNP cord-005207-02wmt2e9 54 36 - - HYPH cord-005207-02wmt2e9 54 37 B b NN cord-005207-02wmt2e9 54 38 - - HYPH cord-005207-02wmt2e9 54 39 galactoside galactoside NNP cord-005207-02wmt2e9 54 40 ( ( -LRB- cord-005207-02wmt2e9 54 41 20 20 CD cord-005207-02wmt2e9 54 42 mg mg NNP cord-005207-02wmt2e9 54 43 / / SYM cord-005207-02wmt2e9 54 44 ml ml NN cord-005207-02wmt2e9 54 45 ) ) -RRB- cord-005207-02wmt2e9 54 46 ( ( -LRB- cord-005207-02wmt2e9 54 47 Invitrogen Invitrogen NNP cord-005207-02wmt2e9 54 48 ) ) -RRB- cord-005207-02wmt2e9 54 49 and and CC cord-005207-02wmt2e9 54 50 incubated incubate VBN cord-005207-02wmt2e9 54 51 overnight overnight RB cord-005207-02wmt2e9 54 52 at at IN cord-005207-02wmt2e9 54 53 37 37 CD cord-005207-02wmt2e9 54 54 C. C. NNP cord-005207-02wmt2e9 54 55 Transformed transform VBN cord-005207-02wmt2e9 54 56 colonies colony NNS cord-005207-02wmt2e9 54 57 were be VBD cord-005207-02wmt2e9 54 58 cultured culture VBN cord-005207-02wmt2e9 54 59 in in IN cord-005207-02wmt2e9 54 60 LB LB NNP cord-005207-02wmt2e9 54 61 broth broth NN cord-005207-02wmt2e9 54 62 with with IN cord-005207-02wmt2e9 54 63 ampicillin ampicillin NN cord-005207-02wmt2e9 54 64 ( ( -LRB- cord-005207-02wmt2e9 54 65 50 50 CD cord-005207-02wmt2e9 54 66 mg mg NNP cord-005207-02wmt2e9 54 67 / / SYM cord-005207-02wmt2e9 54 68 ml ml NN cord-005207-02wmt2e9 54 69 ) ) -RRB- cord-005207-02wmt2e9 54 70 by by IN cord-005207-02wmt2e9 54 71 shaking shake VBG cord-005207-02wmt2e9 54 72 at at IN cord-005207-02wmt2e9 54 73 220 220 CD cord-005207-02wmt2e9 54 74 rpm rpm NN cord-005207-02wmt2e9 54 75 , , , cord-005207-02wmt2e9 54 76 overnight overnight RB cord-005207-02wmt2e9 54 77 , , , cord-005207-02wmt2e9 54 78 at at IN cord-005207-02wmt2e9 54 79 37 37 CD cord-005207-02wmt2e9 54 80 C c NN cord-005207-02wmt2e9 54 81 , , , cord-005207-02wmt2e9 54 82 and and CC cord-005207-02wmt2e9 54 83 were be VBD cord-005207-02wmt2e9 54 84 subjected subject VBN cord-005207-02wmt2e9 54 85 to to IN cord-005207-02wmt2e9 54 86 DNA dna NN cord-005207-02wmt2e9 54 87 extraction extraction NN cord-005207-02wmt2e9 54 88 by by IN cord-005207-02wmt2e9 54 89 alkaline alkaline JJ cord-005207-02wmt2e9 54 90 - - HYPH cord-005207-02wmt2e9 54 91 lysis lysis NN cord-005207-02wmt2e9 54 92 , , , cord-005207-02wmt2e9 54 93 restriction restriction NN cord-005207-02wmt2e9 54 94 enzyme enzyme NN cord-005207-02wmt2e9 54 95 digestion digestion NN cord-005207-02wmt2e9 54 96 and and CC cord-005207-02wmt2e9 54 97 electrophoresis electrophoresis NN cord-005207-02wmt2e9 54 98 in in IN cord-005207-02wmt2e9 54 99 1 1 CD cord-005207-02wmt2e9 54 100 % % NN cord-005207-02wmt2e9 54 101 agarose agarose NN cord-005207-02wmt2e9 54 102 gel gel NN cord-005207-02wmt2e9 54 103 for for IN cord-005207-02wmt2e9 54 104 the the DT cord-005207-02wmt2e9 54 105 identification identification NN cord-005207-02wmt2e9 54 106 of of IN cord-005207-02wmt2e9 54 107 recombinant recombinant JJ cord-005207-02wmt2e9 54 108 DNA dna NN cord-005207-02wmt2e9 54 109 clones clone NNS cord-005207-02wmt2e9 54 110 . . . cord-005207-02wmt2e9 55 1 Nucleotide nucleotide JJ cord-005207-02wmt2e9 55 2 sequencing sequencing NN cord-005207-02wmt2e9 55 3 was be VBD cord-005207-02wmt2e9 55 4 done do VBN cord-005207-02wmt2e9 55 5 for for IN cord-005207-02wmt2e9 55 6 the the DT cord-005207-02wmt2e9 55 7 N n CD cord-005207-02wmt2e9 55 8 generecombinant generecombinant NN cord-005207-02wmt2e9 55 9 DNA dna NN cord-005207-02wmt2e9 55 10 clones clone NNS cord-005207-02wmt2e9 55 11 using use VBG cord-005207-02wmt2e9 55 12 Dye Dye NNP cord-005207-02wmt2e9 55 13 Terminator Terminator NNP cord-005207-02wmt2e9 55 14 Cycle Cycle NNP cord-005207-02wmt2e9 55 15 Sequencing Sequencing NNP cord-005207-02wmt2e9 55 16 kit kit NN cord-005207-02wmt2e9 55 17 ( ( -LRB- cord-005207-02wmt2e9 55 18 Perkin Perkin NNP cord-005207-02wmt2e9 55 19 - - HYPH cord-005207-02wmt2e9 55 20 Elmer Elmer NNP cord-005207-02wmt2e9 55 21 ) ) -RRB- cord-005207-02wmt2e9 55 22 by by IN cord-005207-02wmt2e9 55 23 the the DT cord-005207-02wmt2e9 55 24 automatic automatic JJ cord-005207-02wmt2e9 55 25 sequencer sequencer NN cord-005207-02wmt2e9 55 26 ( ( -LRB- cord-005207-02wmt2e9 55 27 ABI ABI NNP cord-005207-02wmt2e9 55 28 prism prism NN cord-005207-02wmt2e9 55 29 377 377 CD cord-005207-02wmt2e9 55 30 , , , cord-005207-02wmt2e9 55 31 Advanced Advanced NNP cord-005207-02wmt2e9 55 32 Biotechnologies Biotechnologies NNPS cord-005207-02wmt2e9 55 33 , , , cord-005207-02wmt2e9 55 34 USA USA NNP cord-005207-02wmt2e9 55 35 ) ) -RRB- cord-005207-02wmt2e9 55 36 . . . cord-005207-02wmt2e9 56 1 The the DT cord-005207-02wmt2e9 56 2 sequences sequence NNS cord-005207-02wmt2e9 56 3 of of IN cord-005207-02wmt2e9 56 4 nucleotides nucleotide NNS cord-005207-02wmt2e9 56 5 and and CC cord-005207-02wmt2e9 56 6 deduced deduce VBN cord-005207-02wmt2e9 56 7 amino amino JJ cord-005207-02wmt2e9 56 8 acids acid NNS cord-005207-02wmt2e9 56 9 were be VBD cord-005207-02wmt2e9 56 10 analyzed analyze VBN cord-005207-02wmt2e9 56 11 by by IN cord-005207-02wmt2e9 56 12 ClustalW ClustalW NNP cord-005207-02wmt2e9 56 13 , , , cord-005207-02wmt2e9 56 14 version version NN cord-005207-02wmt2e9 56 15 1.82 1.82 CD cord-005207-02wmt2e9 56 16 using use VBG cord-005207-02wmt2e9 56 17 data datum NNS cord-005207-02wmt2e9 56 18 available available JJ cord-005207-02wmt2e9 56 19 from from IN cord-005207-02wmt2e9 56 20 GenBank GenBank NNP cord-005207-02wmt2e9 56 21 and and CC cord-005207-02wmt2e9 56 22 the the DT cord-005207-02wmt2e9 56 23 European European NNP cord-005207-02wmt2e9 56 24 Molecular Molecular NNP cord-005207-02wmt2e9 56 25 Biology Biology NNP cord-005207-02wmt2e9 56 26 Laboratory Laboratory NNP cord-005207-02wmt2e9 56 27 ( ( -LRB- cord-005207-02wmt2e9 56 28 EMBL EMBL NNP cord-005207-02wmt2e9 56 29 ) ) -RRB- cord-005207-02wmt2e9 56 30 . . . cord-005207-02wmt2e9 57 1 N n NN cord-005207-02wmt2e9 57 2 gene gene NN cord-005207-02wmt2e9 57 3 nucleotide nucleotide NN cord-005207-02wmt2e9 57 4 and and CC cord-005207-02wmt2e9 57 5 amino amino NN cord-005207-02wmt2e9 57 6 acid acid NN cord-005207-02wmt2e9 57 7 sequences sequence NNS cord-005207-02wmt2e9 57 8 of of IN cord-005207-02wmt2e9 57 9 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 57 10 were be VBD cord-005207-02wmt2e9 57 11 compared compare VBN cord-005207-02wmt2e9 57 12 with with IN cord-005207-02wmt2e9 57 13 CV777 CV777 NNP cord-005207-02wmt2e9 57 14 and and CC cord-005207-02wmt2e9 57 15 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 57 16 [ [ -LRB- cord-005207-02wmt2e9 57 17 10 10 CD cord-005207-02wmt2e9 57 18 ] ] -RRB- cord-005207-02wmt2e9 58 1 ( ( -LRB- cord-005207-02wmt2e9 58 2 EMBL EMBL NNP cord-005207-02wmt2e9 58 3 accession accession NN cord-005207-02wmt2e9 58 4 No no UH cord-005207-02wmt2e9 58 5 . . . cord-005207-02wmt2e9 59 1 Z14976 Z14976 NNP cord-005207-02wmt2e9 59 2 ) ) -RRB- cord-005207-02wmt2e9 59 3 . . . cord-005207-02wmt2e9 60 1 The the DT cord-005207-02wmt2e9 60 2 protein protein NN cord-005207-02wmt2e9 60 3 chemistry chemistry NN cord-005207-02wmt2e9 60 4 of of IN cord-005207-02wmt2e9 60 5 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 61 1 amino amino JJ cord-005207-02wmt2e9 61 2 acids acid NNS cord-005207-02wmt2e9 61 3 was be VBD cord-005207-02wmt2e9 61 4 analyzed analyze VBN cord-005207-02wmt2e9 61 5 using use VBG cord-005207-02wmt2e9 61 6 protein protein NN cord-005207-02wmt2e9 61 7 statistic statistic NN cord-005207-02wmt2e9 61 8 programs program NNS cord-005207-02wmt2e9 61 9 PEPSTATS PEPSTATS NNP cord-005207-02wmt2e9 61 10 ( ( -LRB- cord-005207-02wmt2e9 61 11 Pasteur Pasteur NNP cord-005207-02wmt2e9 61 12 Institute Institute NNP cord-005207-02wmt2e9 61 13 , , , cord-005207-02wmt2e9 61 14 France France NNP cord-005207-02wmt2e9 61 15 ) ) -RRB- cord-005207-02wmt2e9 61 16 and and CC cord-005207-02wmt2e9 61 17 PredictProtein PredictProtein NNP cord-005207-02wmt2e9 61 18 ( ( -LRB- cord-005207-02wmt2e9 61 19 EMBL EMBL NNP cord-005207-02wmt2e9 61 20 ) ) -RRB- cord-005207-02wmt2e9 61 21 . . . cord-005207-02wmt2e9 62 1 In in IN cord-005207-02wmt2e9 62 2 the the DT cord-005207-02wmt2e9 62 3 synthesis synthesis NN cord-005207-02wmt2e9 62 4 of of IN cord-005207-02wmt2e9 62 5 ds ds NNP cord-005207-02wmt2e9 62 6 - - HYPH cord-005207-02wmt2e9 62 7 cDNA cDNA NNP cord-005207-02wmt2e9 62 8 of of IN cord-005207-02wmt2e9 62 9 the the DT cord-005207-02wmt2e9 62 10 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 63 1 N N NNP cord-005207-02wmt2e9 63 2 gene gene NN cord-005207-02wmt2e9 63 3 , , , cord-005207-02wmt2e9 63 4 a a DT cord-005207-02wmt2e9 63 5 DNA dna NN cord-005207-02wmt2e9 63 6 fragment fragment NN cord-005207-02wmt2e9 63 7 of of IN cord-005207-02wmt2e9 63 8 1.4 1.4 CD cord-005207-02wmt2e9 63 9 kb kb NNS cord-005207-02wmt2e9 63 10 in in IN cord-005207-02wmt2e9 63 11 approximate approximate NN cord-005207-02wmt2e9 63 12 was be VBD cord-005207-02wmt2e9 63 13 amplified amplify VBN cord-005207-02wmt2e9 63 14 by by IN cord-005207-02wmt2e9 63 15 RT RT NNP cord-005207-02wmt2e9 63 16 - - HYPH cord-005207-02wmt2e9 63 17 PCR PCR NNP cord-005207-02wmt2e9 63 18 using use VBG cord-005207-02wmt2e9 63 19 primers primer NNS cord-005207-02wmt2e9 63 20 specific specific JJ cord-005207-02wmt2e9 63 21 to to IN cord-005207-02wmt2e9 63 22 N n CD cord-005207-02wmt2e9 63 23 gene gene NN cord-005207-02wmt2e9 63 24 of of IN cord-005207-02wmt2e9 63 25 PEDV PEDV NNP cord-005207-02wmt2e9 63 26 . . . cord-005207-02wmt2e9 64 1 The the DT cord-005207-02wmt2e9 64 2 DNA dna NN cord-005207-02wmt2e9 64 3 was be VBD cord-005207-02wmt2e9 64 4 cloned clone VBN cord-005207-02wmt2e9 64 5 into into IN cord-005207-02wmt2e9 64 6 pTZ19R ptz19r JJ cord-005207-02wmt2e9 64 7 vector vector NN cord-005207-02wmt2e9 64 8 DNA dna NN cord-005207-02wmt2e9 64 9 ( ( -LRB- cord-005207-02wmt2e9 64 10 Fig Fig NNP cord-005207-02wmt2e9 64 11 . . . cord-005207-02wmt2e9 64 12 1 1 CD cord-005207-02wmt2e9 64 13 ) ) -RRB- cord-005207-02wmt2e9 64 14 and and CC cord-005207-02wmt2e9 64 15 subjected subject VBN cord-005207-02wmt2e9 64 16 to to IN cord-005207-02wmt2e9 64 17 sequencing sequence VBG cord-005207-02wmt2e9 64 18 . . . cord-005207-02wmt2e9 65 1 The the DT cord-005207-02wmt2e9 65 2 nucleotide nucleotide JJ cord-005207-02wmt2e9 65 3 sequence sequence NN cord-005207-02wmt2e9 65 4 encoding encode VBG cord-005207-02wmt2e9 65 5 the the DT cord-005207-02wmt2e9 65 6 entire entire JJ cord-005207-02wmt2e9 65 7 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 66 1 N n NN cord-005207-02wmt2e9 66 2 gene gene NN cord-005207-02wmt2e9 66 3 was be VBD cord-005207-02wmt2e9 66 4 1326 1326 CD cord-005207-02wmt2e9 66 5 bases basis NNS cord-005207-02wmt2e9 66 6 in in IN cord-005207-02wmt2e9 66 7 length length NN cord-005207-02wmt2e9 66 8 and and CC cord-005207-02wmt2e9 66 9 contained contain VBD cord-005207-02wmt2e9 66 10 a a DT cord-005207-02wmt2e9 66 11 single single JJ cord-005207-02wmt2e9 66 12 ORF orf NN cord-005207-02wmt2e9 66 13 . . . cord-005207-02wmt2e9 67 1 The the DT cord-005207-02wmt2e9 67 2 gene gene NN cord-005207-02wmt2e9 67 3 had have VBD cord-005207-02wmt2e9 67 4 46 46 CD cord-005207-02wmt2e9 67 5 and and CC cord-005207-02wmt2e9 67 6 48 48 CD cord-005207-02wmt2e9 67 7 nucleotide nucleotide JJ cord-005207-02wmt2e9 67 8 mismatches mismatch NNS cord-005207-02wmt2e9 67 9 compared compare VBN cord-005207-02wmt2e9 67 10 to to IN cord-005207-02wmt2e9 67 11 CV777 CV777 NNP cord-005207-02wmt2e9 67 12 and and CC cord-005207-02wmt2e9 67 13 Br1/ br1/ NN cord-005207-02wmt2e9 67 14 87 87 CD cord-005207-02wmt2e9 67 15 , , , cord-005207-02wmt2e9 67 16 respectively respectively RB cord-005207-02wmt2e9 67 17 ( ( -LRB- cord-005207-02wmt2e9 67 18 Fig fig NN cord-005207-02wmt2e9 67 19 . . NNP cord-005207-02wmt2e9 67 20 2 2 CD cord-005207-02wmt2e9 67 21 ) ) -RRB- cord-005207-02wmt2e9 67 22 . . . cord-005207-02wmt2e9 68 1 It -PRON- PRP cord-005207-02wmt2e9 68 2 consisted consist VBD cord-005207-02wmt2e9 68 3 of of IN cord-005207-02wmt2e9 68 4 405 405 CD cord-005207-02wmt2e9 68 5 adenine adenine NN cord-005207-02wmt2e9 68 6 ( ( -LRB- cord-005207-02wmt2e9 68 7 30.5 30.5 CD cord-005207-02wmt2e9 68 8 % % NN cord-005207-02wmt2e9 68 9 ) ) -RRB- cord-005207-02wmt2e9 68 10 , , , cord-005207-02wmt2e9 68 11 293 293 CD cord-005207-02wmt2e9 68 12 cytosine cytosine NN cord-005207-02wmt2e9 68 13 ( ( -LRB- cord-005207-02wmt2e9 68 14 22.1 22.1 CD cord-005207-02wmt2e9 68 15 % % NN cord-005207-02wmt2e9 68 16 ) ) -RRB- cord-005207-02wmt2e9 68 17 , , , cord-005207-02wmt2e9 68 18 334 334 CD cord-005207-02wmt2e9 68 19 guanine guanine NN cord-005207-02wmt2e9 68 20 ( ( -LRB- cord-005207-02wmt2e9 68 21 25.2 25.2 CD cord-005207-02wmt2e9 68 22 % % NN cord-005207-02wmt2e9 68 23 ) ) -RRB- cord-005207-02wmt2e9 68 24 and and CC cord-005207-02wmt2e9 68 25 294 294 CD cord-005207-02wmt2e9 68 26 thymine thymine NN cord-005207-02wmt2e9 68 27 ( ( -LRB- cord-005207-02wmt2e9 68 28 22.2 22.2 CD cord-005207-02wmt2e9 68 29 % % NN cord-005207-02wmt2e9 68 30 ) ) -RRB- cord-005207-02wmt2e9 68 31 nucleotides nucleotide NNS cord-005207-02wmt2e9 68 32 , , , cord-005207-02wmt2e9 68 33 and and CC cord-005207-02wmt2e9 68 34 a a DT cord-005207-02wmt2e9 68 35 GC GC NNP cord-005207-02wmt2e9 68 36 content content NN cord-005207-02wmt2e9 68 37 of of IN cord-005207-02wmt2e9 68 38 47.3 47.3 CD cord-005207-02wmt2e9 68 39 % % NN cord-005207-02wmt2e9 68 40 . . . cord-005207-02wmt2e9 69 1 The the DT cord-005207-02wmt2e9 69 2 gene gene NN cord-005207-02wmt2e9 69 3 showed show VBD cord-005207-02wmt2e9 69 4 96.5 96.5 CD cord-005207-02wmt2e9 69 5 % % NN cord-005207-02wmt2e9 69 6 and and CC cord-005207-02wmt2e9 69 7 96.4 96.4 CD cord-005207-02wmt2e9 69 8 % % NN cord-005207-02wmt2e9 69 9 nucleotide nucleotide JJ cord-005207-02wmt2e9 69 10 sequence sequence NN cord-005207-02wmt2e9 69 11 homology homology NN cord-005207-02wmt2e9 69 12 to to IN cord-005207-02wmt2e9 69 13 that that DT cord-005207-02wmt2e9 69 14 of of IN cord-005207-02wmt2e9 69 15 CV777 cv777 CD cord-005207-02wmt2e9 70 1 and and CC cord-005207-02wmt2e9 70 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 70 3 , , , cord-005207-02wmt2e9 70 4 respectively respectively RB cord-005207-02wmt2e9 70 5 . . . cord-005207-02wmt2e9 71 1 The the DT cord-005207-02wmt2e9 71 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 71 3 N N NNP cord-005207-02wmt2e9 71 4 gene gene NN cord-005207-02wmt2e9 71 5 encoded encode VBD cord-005207-02wmt2e9 71 6 a a DT cord-005207-02wmt2e9 71 7 protein protein NN cord-005207-02wmt2e9 71 8 of of IN cord-005207-02wmt2e9 71 9 441 441 CD cord-005207-02wmt2e9 71 10 amino amino JJ cord-005207-02wmt2e9 71 11 acids acid NNS cord-005207-02wmt2e9 71 12 with with IN cord-005207-02wmt2e9 71 13 predicted predict VBN cord-005207-02wmt2e9 71 14 M M NNP cord-005207-02wmt2e9 71 15 r r NNP cord-005207-02wmt2e9 71 16 of of IN cord-005207-02wmt2e9 71 17 49 49 CD cord-005207-02wmt2e9 71 18 kDa kDa NNS cord-005207-02wmt2e9 71 19 . . . cord-005207-02wmt2e9 72 1 There there EX cord-005207-02wmt2e9 72 2 were be VBD cord-005207-02wmt2e9 72 3 seven seven CD cord-005207-02wmt2e9 72 4 potential potential JJ cord-005207-02wmt2e9 72 5 threonine threonine NN cord-005207-02wmt2e9 72 6 ( ( -LRB- cord-005207-02wmt2e9 72 7 T)-or T)-or NNP cord-005207-02wmt2e9 72 8 serine serine NN cord-005207-02wmt2e9 72 9 ( ( -LRB- cord-005207-02wmt2e9 72 10 S)-linked S)-linked NNP cord-005207-02wmt2e9 72 11 phosphorylation phosphorylation NN cord-005207-02wmt2e9 72 12 sites site NNS cord-005207-02wmt2e9 72 13 by by IN cord-005207-02wmt2e9 72 14 each each DT cord-005207-02wmt2e9 72 15 protein protein NN cord-005207-02wmt2e9 72 16 kinase kinase NN cord-005207-02wmt2e9 72 17 C c NN cord-005207-02wmt2e9 72 18 and and CC cord-005207-02wmt2e9 72 19 casein casein NN cord-005207-02wmt2e9 72 20 kinase kinase NNP cord-005207-02wmt2e9 72 21 II II NNP cord-005207-02wmt2e9 72 22 recognized recognize VBN cord-005207-02wmt2e9 72 23 in in IN cord-005207-02wmt2e9 72 24 the the DT cord-005207-02wmt2e9 72 25 protein protein NN cord-005207-02wmt2e9 72 26 . . . cord-005207-02wmt2e9 73 1 The the DT cord-005207-02wmt2e9 73 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 73 3 N N NNP cord-005207-02wmt2e9 73 4 protein protein NN cord-005207-02wmt2e9 73 5 had have VBD cord-005207-02wmt2e9 73 6 14 14 CD cord-005207-02wmt2e9 73 7 amino amino NN cord-005207-02wmt2e9 73 8 acid acid NN cord-005207-02wmt2e9 73 9 mismatches mismatch NNS cord-005207-02wmt2e9 73 10 compared compare VBN cord-005207-02wmt2e9 73 11 to to IN cord-005207-02wmt2e9 73 12 those those DT cord-005207-02wmt2e9 73 13 of of IN cord-005207-02wmt2e9 73 14 CV777 CV777 NNP cord-005207-02wmt2e9 74 1 and and CC cord-005207-02wmt2e9 74 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 74 3 ( ( -LRB- cord-005207-02wmt2e9 74 4 Fig Fig NNP cord-005207-02wmt2e9 74 5 . . . cord-005207-02wmt2e9 74 6 3 3 CD cord-005207-02wmt2e9 74 7 ) ) -RRB- cord-005207-02wmt2e9 75 1 and and CC cord-005207-02wmt2e9 75 2 showed show VBD cord-005207-02wmt2e9 75 3 96.8 96.8 CD cord-005207-02wmt2e9 75 4 % % NN cord-005207-02wmt2e9 75 5 amino amino NN cord-005207-02wmt2e9 75 6 acid acid NN cord-005207-02wmt2e9 75 7 sequence sequence NN cord-005207-02wmt2e9 75 8 identity identity NN cord-005207-02wmt2e9 75 9 with with IN cord-005207-02wmt2e9 75 10 these these DT cord-005207-02wmt2e9 75 11 strains strain NNS cord-005207-02wmt2e9 75 12 . . . cord-005207-02wmt2e9 76 1 Bridgen Bridgen NNP cord-005207-02wmt2e9 76 2 et et NNP cord-005207-02wmt2e9 76 3 al al NNP cord-005207-02wmt2e9 76 4 . . . cord-005207-02wmt2e9 77 1 [ [ -LRB- cord-005207-02wmt2e9 77 2 10 10 CD cord-005207-02wmt2e9 77 3 ] ] -RRB- cord-005207-02wmt2e9 77 4 previously previously RB cord-005207-02wmt2e9 77 5 cloned clone VBD cord-005207-02wmt2e9 77 6 a a DT cord-005207-02wmt2e9 77 7 gene gene NN cord-005207-02wmt2e9 77 8 of of IN cord-005207-02wmt2e9 77 9 1326 1326 CD cord-005207-02wmt2e9 77 10 nucleotides nucleotide NNS cord-005207-02wmt2e9 77 11 in in IN cord-005207-02wmt2e9 77 12 a a DT cord-005207-02wmt2e9 77 13 single single JJ cord-005207-02wmt2e9 77 14 large large JJ cord-005207-02wmt2e9 77 15 ORF ORF NNP cord-005207-02wmt2e9 77 16 capable capable JJ cord-005207-02wmt2e9 77 17 of of IN cord-005207-02wmt2e9 77 18 encoding encode VBG cord-005207-02wmt2e9 77 19 a a DT cord-005207-02wmt2e9 77 20 441 441 CD cord-005207-02wmt2e9 77 21 amino amino NN cord-005207-02wmt2e9 77 22 acid acid NN cord-005207-02wmt2e9 77 23 protein protein NN cord-005207-02wmt2e9 77 24 of of IN cord-005207-02wmt2e9 77 25 49 49 CD cord-005207-02wmt2e9 77 26 kDa kDa NNS cord-005207-02wmt2e9 77 27 from from IN cord-005207-02wmt2e9 77 28 PEDV PEDV NNP cord-005207-02wmt2e9 77 29 CV777 CV777 NNP cord-005207-02wmt2e9 78 1 and and CC cord-005207-02wmt2e9 78 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 78 3 , , , cord-005207-02wmt2e9 78 4 which which WDT cord-005207-02wmt2e9 78 5 were be VBD cord-005207-02wmt2e9 78 6 very very RB cord-005207-02wmt2e9 78 7 similar similar JJ cord-005207-02wmt2e9 78 8 Nucleotide Nucleotide NNP cord-005207-02wmt2e9 78 9 Sequence sequence NN cord-005207-02wmt2e9 78 10 of of IN cord-005207-02wmt2e9 78 11 PEDV PEDV NNP cord-005207-02wmt2e9 78 12 N N NNP cord-005207-02wmt2e9 78 13 gene gene NN cord-005207-02wmt2e9 78 14 in in IN cord-005207-02wmt2e9 78 15 both both DT cord-005207-02wmt2e9 78 16 length length NN cord-005207-02wmt2e9 78 17 and and CC cord-005207-02wmt2e9 78 18 sequence sequence NN cord-005207-02wmt2e9 78 19 to to IN cord-005207-02wmt2e9 78 20 coronavirus coronavirus NN cord-005207-02wmt2e9 78 21 N n CD cord-005207-02wmt2e9 78 22 proteins protein NNS cord-005207-02wmt2e9 78 23 , , , cord-005207-02wmt2e9 78 24 and and CC cord-005207-02wmt2e9 78 25 therefore therefore RB cord-005207-02wmt2e9 78 26 represented represent VBD cord-005207-02wmt2e9 78 27 it -PRON- PRP cord-005207-02wmt2e9 78 28 as as IN cord-005207-02wmt2e9 78 29 the the DT cord-005207-02wmt2e9 78 30 PEDV PEDV NNP cord-005207-02wmt2e9 78 31 N N NNP cord-005207-02wmt2e9 78 32 gene gene NN cord-005207-02wmt2e9 78 33 . . . cord-005207-02wmt2e9 79 1 In in IN cord-005207-02wmt2e9 79 2 the the DT cord-005207-02wmt2e9 79 3 present present JJ cord-005207-02wmt2e9 79 4 study study NN cord-005207-02wmt2e9 79 5 , , , cord-005207-02wmt2e9 79 6 the the DT cord-005207-02wmt2e9 79 7 N n CD cord-005207-02wmt2e9 79 8 gene gene NN cord-005207-02wmt2e9 79 9 of of IN cord-005207-02wmt2e9 79 10 the the DT cord-005207-02wmt2e9 79 11 PEDV PEDV NNP cord-005207-02wmt2e9 79 12 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 79 13 was be VBD cord-005207-02wmt2e9 79 14 cloned clone VBN cord-005207-02wmt2e9 79 15 and and CC cord-005207-02wmt2e9 79 16 sequencing sequencing NN cord-005207-02wmt2e9 79 17 was be VBD cord-005207-02wmt2e9 79 18 done do VBN cord-005207-02wmt2e9 79 19 for for IN cord-005207-02wmt2e9 79 20 the the DT cord-005207-02wmt2e9 79 21 cDNA cdna NN cord-005207-02wmt2e9 79 22 clones clone NNS cord-005207-02wmt2e9 79 23 . . . cord-005207-02wmt2e9 80 1 The the DT cord-005207-02wmt2e9 80 2 resulting result VBG cord-005207-02wmt2e9 80 3 sequence sequence NN cord-005207-02wmt2e9 80 4 data datum NNS cord-005207-02wmt2e9 80 5 showed show VBD cord-005207-02wmt2e9 80 6 a a DT cord-005207-02wmt2e9 80 7 single single JJ cord-005207-02wmt2e9 80 8 ORF orf NN cord-005207-02wmt2e9 80 9 of of IN cord-005207-02wmt2e9 80 10 1326 1326 CD cord-005207-02wmt2e9 80 11 nucleotides nucleotide NNS cord-005207-02wmt2e9 80 12 encoding encode VBG cord-005207-02wmt2e9 80 13 a a DT cord-005207-02wmt2e9 80 14 protein protein NN cord-005207-02wmt2e9 80 15 of of IN cord-005207-02wmt2e9 80 16 441 441 CD cord-005207-02wmt2e9 80 17 amino amino JJ cord-005207-02wmt2e9 80 18 acids acid NNS cord-005207-02wmt2e9 80 19 with with IN cord-005207-02wmt2e9 80 20 M M NNP cord-005207-02wmt2e9 80 21 r r NN cord-005207-02wmt2e9 80 22 of of IN cord-005207-02wmt2e9 80 23 49 49 CD cord-005207-02wmt2e9 80 24 kDa kDa NNS cord-005207-02wmt2e9 80 25 predicted predict VBN cord-005207-02wmt2e9 80 26 by by IN cord-005207-02wmt2e9 80 27 PEPSTATS PEPSTATS NNP cord-005207-02wmt2e9 80 28 program program NN cord-005207-02wmt2e9 80 29 . . . cord-005207-02wmt2e9 81 1 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 82 1 N n NN cord-005207-02wmt2e9 82 2 gene gene NN cord-005207-02wmt2e9 82 3 also also RB cord-005207-02wmt2e9 82 4 had have VBD cord-005207-02wmt2e9 82 5 96.8 96.8 CD cord-005207-02wmt2e9 82 6 % % NN cord-005207-02wmt2e9 82 7 amino amino NN cord-005207-02wmt2e9 82 8 acid acid NN cord-005207-02wmt2e9 82 9 sequence sequence NN cord-005207-02wmt2e9 82 10 identity identity NN cord-005207-02wmt2e9 82 11 with with IN cord-005207-02wmt2e9 82 12 that that DT cord-005207-02wmt2e9 82 13 of of IN cord-005207-02wmt2e9 82 14 CV777 cv777 CD cord-005207-02wmt2e9 83 1 and and CC cord-005207-02wmt2e9 83 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 83 3 [ [ -LRB- cord-005207-02wmt2e9 83 4 10 10 CD cord-005207-02wmt2e9 83 5 ] ] -RRB- cord-005207-02wmt2e9 83 6 , , , cord-005207-02wmt2e9 83 7 although although IN cord-005207-02wmt2e9 83 8 there there EX cord-005207-02wmt2e9 83 9 were be VBD cord-005207-02wmt2e9 83 10 14 14 CD cord-005207-02wmt2e9 83 11 amino amino NN cord-005207-02wmt2e9 83 12 acid acid NN cord-005207-02wmt2e9 83 13 mismatches mismatch NNS cord-005207-02wmt2e9 83 14 recognized recognize VBN cord-005207-02wmt2e9 83 15 . . . cord-005207-02wmt2e9 84 1 Therefore therefore RB cord-005207-02wmt2e9 84 2 , , , cord-005207-02wmt2e9 84 3 the the DT cord-005207-02wmt2e9 84 4 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 85 1 N N NNP cord-005207-02wmt2e9 85 2 protein protein NN cord-005207-02wmt2e9 85 3 revealed reveal VBD cord-005207-02wmt2e9 85 4 the the DT cord-005207-02wmt2e9 85 5 same same JJ cord-005207-02wmt2e9 85 6 features feature NNS cord-005207-02wmt2e9 85 7 for for IN cord-005207-02wmt2e9 85 8 the the DT cord-005207-02wmt2e9 85 9 nucleotide nucleotide JJ cord-005207-02wmt2e9 85 10 and and CC cord-005207-02wmt2e9 85 11 putative putative JJ cord-005207-02wmt2e9 85 12 amino amino NN cord-005207-02wmt2e9 85 13 acid acid NN cord-005207-02wmt2e9 85 14 sequences sequence NNS cord-005207-02wmt2e9 85 15 in in IN cord-005207-02wmt2e9 85 16 the the DT cord-005207-02wmt2e9 85 17 CV777 CV777 NNP cord-005207-02wmt2e9 86 1 and and CC cord-005207-02wmt2e9 86 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 86 3 , , , cord-005207-02wmt2e9 86 4 although although IN cord-005207-02wmt2e9 86 5 PEDV PEDV NNP cord-005207-02wmt2e9 86 6 N N NNP cord-005207-02wmt2e9 86 7 protein protein NN cord-005207-02wmt2e9 86 8 is be VBZ cord-005207-02wmt2e9 86 9 known know VBN cord-005207-02wmt2e9 86 10 to to TO cord-005207-02wmt2e9 86 11 possess possess VB cord-005207-02wmt2e9 86 12 M M NNP cord-005207-02wmt2e9 86 13 r r NN cord-005207-02wmt2e9 86 14 of of IN cord-005207-02wmt2e9 86 15 55±58 55±58 NNP cord-005207-02wmt2e9 86 16 kDa kDa NNPS cord-005207-02wmt2e9 86 17 by by IN cord-005207-02wmt2e9 86 18 polyacrylamid polyacrylamid NNP cord-005207-02wmt2e9 86 19 gel gel NN cord-005207-02wmt2e9 86 20 electrophoresis electrophoresis NN cord-005207-02wmt2e9 86 21 [ [ -LRB- cord-005207-02wmt2e9 86 22 8 8 CD cord-005207-02wmt2e9 86 23 , , , cord-005207-02wmt2e9 86 24 9 9 CD cord-005207-02wmt2e9 86 25 ] ] -RRB- cord-005207-02wmt2e9 86 26 . . . cord-005207-02wmt2e9 87 1 The the DT cord-005207-02wmt2e9 87 2 PEDV PEDV NNP cord-005207-02wmt2e9 87 3 N N NNP cord-005207-02wmt2e9 87 4 protein protein NN cord-005207-02wmt2e9 87 5 is be VBZ cord-005207-02wmt2e9 87 6 known know VBN cord-005207-02wmt2e9 87 7 as as IN cord-005207-02wmt2e9 87 8 a a DT cord-005207-02wmt2e9 87 9 phosphorylated phosphorylated JJ cord-005207-02wmt2e9 87 10 , , , cord-005207-02wmt2e9 87 11 structural structural JJ cord-005207-02wmt2e9 87 12 protein protein NN cord-005207-02wmt2e9 87 13 associated associate VBN cord-005207-02wmt2e9 87 14 with with IN cord-005207-02wmt2e9 87 15 viral viral JJ cord-005207-02wmt2e9 87 16 genome genome NN cord-005207-02wmt2e9 87 17 [ [ -LRB- cord-005207-02wmt2e9 87 18 1 1 CD cord-005207-02wmt2e9 87 19 , , , cord-005207-02wmt2e9 87 20 3 3 CD cord-005207-02wmt2e9 87 21 , , , cord-005207-02wmt2e9 87 22 8 8 CD cord-005207-02wmt2e9 87 23 , , , cord-005207-02wmt2e9 87 24 9 9 CD cord-005207-02wmt2e9 87 25 ] ] -RRB- cord-005207-02wmt2e9 87 26 , , , cord-005207-02wmt2e9 87 27 which which WDT cord-005207-02wmt2e9 87 28 appears appear VBZ cord-005207-02wmt2e9 87 29 abundantly abundantly RB cord-005207-02wmt2e9 87 30 in in IN cord-005207-02wmt2e9 87 31 virus virus NN cord-005207-02wmt2e9 87 32 - - HYPH cord-005207-02wmt2e9 87 33 infected infect VBN cord-005207-02wmt2e9 87 34 cells cell NNS cord-005207-02wmt2e9 87 35 [ [ -LRB- cord-005207-02wmt2e9 87 36 9 9 CD cord-005207-02wmt2e9 87 37 ] ] -RRB- cord-005207-02wmt2e9 87 38 . . . cord-005207-02wmt2e9 88 1 Therefore therefore RB cord-005207-02wmt2e9 88 2 , , , cord-005207-02wmt2e9 88 3 the the DT cord-005207-02wmt2e9 88 4 appearance appearance NN cord-005207-02wmt2e9 88 5 of of IN cord-005207-02wmt2e9 88 6 N n CD cord-005207-02wmt2e9 88 7 protein protein NN cord-005207-02wmt2e9 88 8 can can MD cord-005207-02wmt2e9 88 9 be be VB cord-005207-02wmt2e9 88 10 a a DT cord-005207-02wmt2e9 88 11 clue clue NN cord-005207-02wmt2e9 88 12 to to IN cord-005207-02wmt2e9 88 13 the the DT cord-005207-02wmt2e9 88 14 replication replication NN cord-005207-02wmt2e9 88 15 of of IN cord-005207-02wmt2e9 88 16 PEDV PEDV NNP cord-005207-02wmt2e9 88 17 and and CC cord-005207-02wmt2e9 88 18 used use VBN cord-005207-02wmt2e9 88 19 for for IN cord-005207-02wmt2e9 88 20 the the DT cord-005207-02wmt2e9 88 21 early early JJ cord-005207-02wmt2e9 88 22 and and CC cord-005207-02wmt2e9 88 23 accurate accurate JJ cord-005207-02wmt2e9 88 24 diagnosis diagnosis NN cord-005207-02wmt2e9 88 25 so so RB cord-005207-02wmt2e9 88 26 far far RB cord-005207-02wmt2e9 88 27 as as IN cord-005207-02wmt2e9 88 28 the the DT cord-005207-02wmt2e9 88 29 virus virus NN cord-005207-02wmt2e9 88 30 replicates replicate VBZ cord-005207-02wmt2e9 88 31 in in IN cord-005207-02wmt2e9 88 32 the the DT cord-005207-02wmt2e9 88 33 infected infected JJ cord-005207-02wmt2e9 88 34 cells cell NNS cord-005207-02wmt2e9 88 35 . . . cord-005207-02wmt2e9 89 1 The the DT cord-005207-02wmt2e9 89 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 89 3 N N NNP cord-005207-02wmt2e9 89 4 protein protein NN cord-005207-02wmt2e9 89 5 had have VBD cord-005207-02wmt2e9 89 6 each each DT cord-005207-02wmt2e9 89 7 seven seven CD cord-005207-02wmt2e9 89 8 potential potential JJ cord-005207-02wmt2e9 89 9 T t NN cord-005207-02wmt2e9 89 10 - - HYPH cord-005207-02wmt2e9 89 11 or or CC cord-005207-02wmt2e9 89 12 S s NN cord-005207-02wmt2e9 89 13 - - HYPH cord-005207-02wmt2e9 89 14 linked link VBN cord-005207-02wmt2e9 89 15 phosphorylation phosphorylation NN cord-005207-02wmt2e9 89 16 sites site NNS cord-005207-02wmt2e9 89 17 by by IN cord-005207-02wmt2e9 89 18 protein protein NN cord-005207-02wmt2e9 89 19 kinase kinase NN cord-005207-02wmt2e9 89 20 C c NN cord-005207-02wmt2e9 89 21 or or CC cord-005207-02wmt2e9 89 22 casein casein NN cord-005207-02wmt2e9 89 23 kinase kinase NN cord-005207-02wmt2e9 89 24 II II NNP cord-005207-02wmt2e9 89 25 , , , cord-005207-02wmt2e9 89 26 respectively respectively RB cord-005207-02wmt2e9 89 27 . . . cord-005207-02wmt2e9 90 1 Similarly similarly RB cord-005207-02wmt2e9 90 2 , , , cord-005207-02wmt2e9 90 3 the the DT cord-005207-02wmt2e9 90 4 CV777 CV777 NNP cord-005207-02wmt2e9 90 5 and and CC cord-005207-02wmt2e9 90 6 Br1/ br1/ NN cord-005207-02wmt2e9 90 7 87 87 CD cord-005207-02wmt2e9 90 8 [ [ -LRB- cord-005207-02wmt2e9 90 9 10 10 CD cord-005207-02wmt2e9 90 10 ] ] -RRB- cord-005207-02wmt2e9 90 11 contained contain VBN cord-005207-02wmt2e9 90 12 six six CD cord-005207-02wmt2e9 90 13 serine serine NN cord-005207-02wmt2e9 90 14 ( ( -LRB- cord-005207-02wmt2e9 90 15 S s NN cord-005207-02wmt2e9 90 16 ) ) -RRB- cord-005207-02wmt2e9 90 17 residues residue NNS cord-005207-02wmt2e9 90 18 as as IN cord-005207-02wmt2e9 90 19 possible possible JJ cord-005207-02wmt2e9 90 20 phosphorylation phosphorylation NN cord-005207-02wmt2e9 90 21 sites site NNS cord-005207-02wmt2e9 90 22 by by IN cord-005207-02wmt2e9 90 23 these these DT cord-005207-02wmt2e9 90 24 enzymes enzyme NNS cord-005207-02wmt2e9 90 25 , , , cord-005207-02wmt2e9 90 26 although although IN cord-005207-02wmt2e9 90 27 some some DT cord-005207-02wmt2e9 90 28 of of IN cord-005207-02wmt2e9 90 29 the the DT cord-005207-02wmt2e9 90 30 S S NNP cord-005207-02wmt2e9 90 31 - - HYPH cord-005207-02wmt2e9 90 32 linked link VBN cord-005207-02wmt2e9 90 33 phosphorylation phosphorylation NN cord-005207-02wmt2e9 90 34 sites site NNS cord-005207-02wmt2e9 90 35 were be VBD cord-005207-02wmt2e9 90 36 different different JJ cord-005207-02wmt2e9 90 37 with with IN cord-005207-02wmt2e9 90 38 those those DT cord-005207-02wmt2e9 90 39 of of IN cord-005207-02wmt2e9 90 40 the the DT cord-005207-02wmt2e9 90 41 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 90 42 . . . cord-005207-02wmt2e9 91 1 In in IN cord-005207-02wmt2e9 91 2 conclusion conclusion NN cord-005207-02wmt2e9 91 3 , , , cord-005207-02wmt2e9 91 4 the the DT cord-005207-02wmt2e9 91 5 full full JJ cord-005207-02wmt2e9 91 6 - - HYPH cord-005207-02wmt2e9 91 7 length length NN cord-005207-02wmt2e9 91 8 nucleotide nucleotide JJ cord-005207-02wmt2e9 91 9 sequence sequence NN cord-005207-02wmt2e9 91 10 in in IN cord-005207-02wmt2e9 91 11 the the DT cord-005207-02wmt2e9 91 12 coding code VBG cord-005207-02wmt2e9 91 13 region region NN cord-005207-02wmt2e9 91 14 of of IN cord-005207-02wmt2e9 91 15 N n CD cord-005207-02wmt2e9 91 16 gene gene NN cord-005207-02wmt2e9 91 17 of of IN cord-005207-02wmt2e9 91 18 PEDV PEDV NNP cord-005207-02wmt2e9 91 19 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 91 20 was be VBD cord-005207-02wmt2e9 91 21 determined determine VBN cord-005207-02wmt2e9 91 22 in in IN cord-005207-02wmt2e9 91 23 the the DT cord-005207-02wmt2e9 91 24 present present JJ cord-005207-02wmt2e9 91 25 study study NN cord-005207-02wmt2e9 91 26 . . . cord-005207-02wmt2e9 92 1 Trials trial NNS cord-005207-02wmt2e9 92 2 were be VBD cord-005207-02wmt2e9 92 3 done do VBN cord-005207-02wmt2e9 92 4 to to TO cord-005207-02wmt2e9 92 5 analyze analyze VB cord-005207-02wmt2e9 92 6 the the DT cord-005207-02wmt2e9 92 7 nucleotide nucleotide JJ cord-005207-02wmt2e9 92 8 and and CC cord-005207-02wmt2e9 92 9 putative putative JJ cord-005207-02wmt2e9 92 10 amino amino NN cord-005207-02wmt2e9 92 11 acid acid NN cord-005207-02wmt2e9 92 12 sequences sequence NNS cord-005207-02wmt2e9 92 13 of of IN cord-005207-02wmt2e9 92 14 the the DT cord-005207-02wmt2e9 92 15 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 92 16 N N NNP cord-005207-02wmt2e9 92 17 gene gene NN cord-005207-02wmt2e9 92 18 comparing compare VBG cord-005207-02wmt2e9 92 19 to to IN cord-005207-02wmt2e9 92 20 those those DT cord-005207-02wmt2e9 92 21 of of IN cord-005207-02wmt2e9 92 22 other other JJ cord-005207-02wmt2e9 92 23 PEDVs pedv NNS cord-005207-02wmt2e9 92 24 . . . cord-005207-02wmt2e9 93 1 However however RB cord-005207-02wmt2e9 93 2 , , , cord-005207-02wmt2e9 93 3 we -PRON- PRP cord-005207-02wmt2e9 93 4 could could MD cord-005207-02wmt2e9 93 5 elucidate elucidate VB cord-005207-02wmt2e9 93 6 molecular molecular JJ cord-005207-02wmt2e9 93 7 properties property NNS cord-005207-02wmt2e9 93 8 of of IN cord-005207-02wmt2e9 93 9 the the DT cord-005207-02wmt2e9 93 10 N n CD cord-005207-02wmt2e9 93 11 gene gene NN cord-005207-02wmt2e9 93 12 by by IN cord-005207-02wmt2e9 93 13 mere mere JJ cord-005207-02wmt2e9 93 14 comparison comparison NN cord-005207-02wmt2e9 93 15 to to IN cord-005207-02wmt2e9 93 16 those those DT cord-005207-02wmt2e9 93 17 of of IN cord-005207-02wmt2e9 93 18 CV777 CV777 NNP cord-005207-02wmt2e9 94 1 and and CC cord-005207-02wmt2e9 94 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 94 3 , , , cord-005207-02wmt2e9 94 4 because because IN cord-005207-02wmt2e9 94 5 the the DT cord-005207-02wmt2e9 94 6 full full JJ cord-005207-02wmt2e9 94 7 - - HYPH cord-005207-02wmt2e9 94 8 length length NN cord-005207-02wmt2e9 94 9 nucleotides nucleotide NNS cord-005207-02wmt2e9 94 10 of of IN cord-005207-02wmt2e9 94 11 the the DT cord-005207-02wmt2e9 94 12 PEDV PEDV NNP cord-005207-02wmt2e9 94 13 N N NNP cord-005207-02wmt2e9 94 14 gene gene NN cord-005207-02wmt2e9 94 15 have have VBP cord-005207-02wmt2e9 94 16 been be VBN cord-005207-02wmt2e9 94 17 determined determine VBN cord-005207-02wmt2e9 94 18 only only RB cord-005207-02wmt2e9 94 19 in in IN cord-005207-02wmt2e9 94 20 these these DT cord-005207-02wmt2e9 94 21 strains strain NNS cord-005207-02wmt2e9 94 22 . . . cord-005207-02wmt2e9 95 1 Nevertheless nevertheless RB cord-005207-02wmt2e9 95 2 , , , cord-005207-02wmt2e9 95 3 it -PRON- PRP cord-005207-02wmt2e9 95 4 was be VBD cord-005207-02wmt2e9 95 5 recognized recognize VBN cord-005207-02wmt2e9 95 6 that that IN cord-005207-02wmt2e9 95 7 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 96 1 N N NNP cord-005207-02wmt2e9 96 2 gene gene NN cord-005207-02wmt2e9 96 3 has have VBZ cord-005207-02wmt2e9 96 4 the the DT cord-005207-02wmt2e9 96 5 minor minor JJ cord-005207-02wmt2e9 96 6 differences difference NNS cord-005207-02wmt2e9 96 7 in in IN cord-005207-02wmt2e9 96 8 the the DT cord-005207-02wmt2e9 96 9 structural structural JJ cord-005207-02wmt2e9 96 10 features feature NNS cord-005207-02wmt2e9 96 11 of of IN cord-005207-02wmt2e9 96 12 putative putative JJ cord-005207-02wmt2e9 96 13 protein protein NN cord-005207-02wmt2e9 96 14 compared compare VBN cord-005207-02wmt2e9 96 15 to to IN cord-005207-02wmt2e9 96 16 those those DT cord-005207-02wmt2e9 96 17 of of IN cord-005207-02wmt2e9 96 18 CV777 CV777 NNP cord-005207-02wmt2e9 97 1 and and CC cord-005207-02wmt2e9 97 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 97 3 . . . cord-005207-02wmt2e9 98 1 This this DT cord-005207-02wmt2e9 98 2 can can MD cord-005207-02wmt2e9 98 3 be be VB cord-005207-02wmt2e9 98 4 the the DT cord-005207-02wmt2e9 98 5 feasible feasible JJ cord-005207-02wmt2e9 98 6 information information NN cord-005207-02wmt2e9 98 7 for for IN cord-005207-02wmt2e9 98 8 the the DT cord-005207-02wmt2e9 98 9 development development NN cord-005207-02wmt2e9 98 10 of of IN cord-005207-02wmt2e9 98 11 genetically genetically RB cord-005207-02wmt2e9 98 12 engineered engineer VBN cord-005207-02wmt2e9 98 13 N n CD cord-005207-02wmt2e9 98 14 protein protein NN cord-005207-02wmt2e9 98 15 for for IN cord-005207-02wmt2e9 98 16 the the DT cord-005207-02wmt2e9 98 17 rapid rapid JJ cord-005207-02wmt2e9 98 18 and and CC cord-005207-02wmt2e9 98 19 accurate accurate JJ cord-005207-02wmt2e9 98 20 diagnosis diagnosis NN cord-005207-02wmt2e9 98 21 of of IN cord-005207-02wmt2e9 98 22 PEDV PEDV NNP cord-005207-02wmt2e9 98 23 infections infection NNS cord-005207-02wmt2e9 98 24 in in IN cord-005207-02wmt2e9 98 25 Korea Korea NNP cord-005207-02wmt2e9 98 26 . . . cord-005207-02wmt2e9 99 1 Moreover moreover RB cord-005207-02wmt2e9 99 2 , , , cord-005207-02wmt2e9 99 3 the the DT cord-005207-02wmt2e9 99 4 genetic genetic JJ cord-005207-02wmt2e9 99 5 information information NN cord-005207-02wmt2e9 99 6 gained gain VBD cord-005207-02wmt2e9 99 7 from from IN cord-005207-02wmt2e9 99 8 the the DT cord-005207-02wmt2e9 99 9 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 100 1 N n NN cord-005207-02wmt2e9 100 2 gene gene NN cord-005207-02wmt2e9 100 3 can can MD cord-005207-02wmt2e9 100 4 be be VB cord-005207-02wmt2e9 100 5 used use VBN cord-005207-02wmt2e9 100 6 for for IN cord-005207-02wmt2e9 100 7 diagnostic diagnostic JJ cord-005207-02wmt2e9 100 8 work work NN cord-005207-02wmt2e9 100 9 such such JJ cord-005207-02wmt2e9 100 10 as as IN cord-005207-02wmt2e9 100 11 PCR PCR NNP cord-005207-02wmt2e9 100 12 and and CC cord-005207-02wmt2e9 100 13 nucleic nucleic JJ cord-005207-02wmt2e9 100 14 acid acid NN cord-005207-02wmt2e9 100 15 hybridization hybridization NN cord-005207-02wmt2e9 100 16 . . . cord-005207-02wmt2e9 101 1 To to IN cord-005207-02wmt2e9 101 2 our -PRON- PRP$ cord-005207-02wmt2e9 101 3 knowledge knowledge NN cord-005207-02wmt2e9 101 4 , , , cord-005207-02wmt2e9 101 5 this this DT cord-005207-02wmt2e9 101 6 is be VBZ cord-005207-02wmt2e9 101 7 the the DT cord-005207-02wmt2e9 101 8 first first JJ cord-005207-02wmt2e9 101 9 published publish VBN cord-005207-02wmt2e9 101 10 report report NN cord-005207-02wmt2e9 101 11 on on IN cord-005207-02wmt2e9 101 12 the the DT cord-005207-02wmt2e9 101 13 full full JJ cord-005207-02wmt2e9 101 14 - - HYPH cord-005207-02wmt2e9 101 15 length length NN cord-005207-02wmt2e9 101 16 nucleotides nucleotide NNS cord-005207-02wmt2e9 101 17 and and CC cord-005207-02wmt2e9 101 18 molecular molecular JJ cord-005207-02wmt2e9 101 19 characteristics characteristic NNS cord-005207-02wmt2e9 101 20 of of IN cord-005207-02wmt2e9 101 21 the the DT cord-005207-02wmt2e9 101 22 N n CD cord-005207-02wmt2e9 101 23 gene gene NN cord-005207-02wmt2e9 101 24 of of IN cord-005207-02wmt2e9 101 25 Korean korean JJ cord-005207-02wmt2e9 101 26 PEDV PEDV NNP cord-005207-02wmt2e9 101 27 isolates isolate NNS cord-005207-02wmt2e9 101 28 . . . cord-005207-02wmt2e9 102 1 and and CC cord-005207-02wmt2e9 102 2 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 102 3 [ [ -LRB- cord-005207-02wmt2e9 102 4 10 10 CD cord-005207-02wmt2e9 102 5 ] ] -RRB- cord-005207-02wmt2e9 102 6 : : : cord-005207-02wmt2e9 102 7 Only only RB cord-005207-02wmt2e9 102 8 the the DT cord-005207-02wmt2e9 102 9 amino amino NN cord-005207-02wmt2e9 102 10 acids acid NNS cord-005207-02wmt2e9 102 11 of of IN cord-005207-02wmt2e9 102 12 CV777 CV777 NNP cord-005207-02wmt2e9 102 13 and and CC cord-005207-02wmt2e9 102 14 Br1/87 Br1/87 NNP cord-005207-02wmt2e9 102 15 which which WDT cord-005207-02wmt2e9 102 16 mismatched mismatch VBD cord-005207-02wmt2e9 103 1 the the DT cord-005207-02wmt2e9 103 2 Chinju99 Chinju99 NNP cord-005207-02wmt2e9 103 3 sequence sequence NN cord-005207-02wmt2e9 103 4 were be VBD cord-005207-02wmt2e9 103 5 included include VBN cord-005207-02wmt2e9 103 6 ; ; : cord-005207-02wmt2e9 103 7 * * NFP cord-005207-02wmt2e9 103 8 , , , cord-005207-02wmt2e9 103 9 translation translation NN cord-005207-02wmt2e9 103 10 termination termination NN cord-005207-02wmt2e9 103 11 ; ; : cord-005207-02wmt2e9 103 12 seven seven CD cord-005207-02wmt2e9 103 13 potential potential JJ cord-005207-02wmt2e9 103 14 threonine threonine NN cord-005207-02wmt2e9 103 15 ( ( -LRB- cord-005207-02wmt2e9 103 16 T)-or T)-or NNP cord-005207-02wmt2e9 103 17 serine serine NN cord-005207-02wmt2e9 103 18 ( ( -LRB- cord-005207-02wmt2e9 103 19 S)-linked S)-linked NNP cord-005207-02wmt2e9 103 20 phosphorylation phosphorylation NN cord-005207-02wmt2e9 103 21 sites site NNS cord-005207-02wmt2e9 103 22 by by IN cord-005207-02wmt2e9 103 23 protein protein NN cord-005207-02wmt2e9 103 24 kinase kinase NN cord-005207-02wmt2e9 103 25 C C NNP cord-005207-02wmt2e9 103 26 were be VBD cord-005207-02wmt2e9 103 27 underlined underline VBN cord-005207-02wmt2e9 103 28 ; ; : cord-005207-02wmt2e9 103 29 seven seven CD cord-005207-02wmt2e9 103 30 potential potential JJ cord-005207-02wmt2e9 103 31 T t NN cord-005207-02wmt2e9 103 32 - - HYPH cord-005207-02wmt2e9 103 33 or or CC cord-005207-02wmt2e9 103 34 S s NN cord-005207-02wmt2e9 103 35 - - HYPH cord-005207-02wmt2e9 103 36 linked link VBN cord-005207-02wmt2e9 103 37 phosphorylation phosphorylation NN cord-005207-02wmt2e9 103 38 sites site NNS cord-005207-02wmt2e9 103 39 by by IN cord-005207-02wmt2e9 103 40 casein casein NN cord-005207-02wmt2e9 103 41 kinase kinase NNP cord-005207-02wmt2e9 103 42 II II NNP cord-005207-02wmt2e9 103 43 were be VBD cord-005207-02wmt2e9 103 44 denoted denote VBN cord-005207-02wmt2e9 103 45 in in IN cord-005207-02wmt2e9 103 46 italic italic NNP cord-005207-02wmt2e9 103 47 . . . cord-005207-02wmt2e9 104 1 Diseases disease NNS cord-005207-02wmt2e9 104 2 of of IN cord-005207-02wmt2e9 104 3 Swine Swine NNP cord-005207-02wmt2e9 104 4 Hagan Hagan NNP cord-005207-02wmt2e9 104 5 and and CC cord-005207-02wmt2e9 104 6 Bruner Bruner NNP cord-005207-02wmt2e9 104 7 's 's POS cord-005207-02wmt2e9 104 8 Microbiology Microbiology NNP cord-005207-02wmt2e9 104 9 and and CC cord-005207-02wmt2e9 104 10 Infectious Infectious NNP cord-005207-02wmt2e9 104 11 Diseases Diseases NNPS cord-005207-02wmt2e9 104 12 of of IN cord-005207-02wmt2e9 104 13 Domestic domestic JJ cord-005207-02wmt2e9 104 14 Animals Animals NNPS cord-005207-02wmt2e9 104 15 Fields Fields NNP cord-005207-02wmt2e9 104 16 Virology Virology NNP cord-005207-02wmt2e9 104 17 . . . cord-005207-02wmt2e9 105 1 Lippincott Lippincott NNP cord-005207-02wmt2e9 105 2 - - HYPH cord-005207-02wmt2e9 105 3 Raven Raven NNP cord-005207-02wmt2e9 105 4 Publishers Publishers NNPS cord-005207-02wmt2e9 105 5 Short Short NNP cord-005207-02wmt2e9 105 6 Potocols Potocols NNP cord-005207-02wmt2e9 105 7 in in IN cord-005207-02wmt2e9 105 8 Molecular Molecular NNP cord-005207-02wmt2e9 105 9 Biology Biology NNP cord-005207-02wmt2e9 106 1 This this DT cord-005207-02wmt2e9 106 2 study study NN cord-005207-02wmt2e9 106 3 was be VBD cord-005207-02wmt2e9 106 4 supported support VBN cord-005207-02wmt2e9 106 5 by by IN cord-005207-02wmt2e9 106 6 a a DT cord-005207-02wmt2e9 106 7 grant grant NN cord-005207-02wmt2e9 106 8 ( ( -LRB- cord-005207-02wmt2e9 106 9 No no NN cord-005207-02wmt2e9 106 10 . . NN cord-005207-02wmt2e9 106 11 981 981 CD cord-005207-02wmt2e9 106 12 - - HYPH cord-005207-02wmt2e9 106 13 0613 0613 CD cord-005207-02wmt2e9 106 14 - - HYPH cord-005207-02wmt2e9 106 15 065 065 CD cord-005207-02wmt2e9 106 16 - - SYM cord-005207-02wmt2e9 106 17 2 2 CD cord-005207-02wmt2e9 106 18 ) ) -RRB- cord-005207-02wmt2e9 106 19 from from IN cord-005207-02wmt2e9 106 20 the the DT cord-005207-02wmt2e9 106 21 Korea Korea NNP cord-005207-02wmt2e9 106 22 Science Science NNP cord-005207-02wmt2e9 106 23 and and CC cord-005207-02wmt2e9 106 24 Engineering Engineering NNP cord-005207-02wmt2e9 106 25 Foundation Foundation NNP cord-005207-02wmt2e9 106 26 ( ( -LRB- cord-005207-02wmt2e9 106 27 KOSEF KOSEF NNP cord-005207-02wmt2e9 106 28 ) ) -RRB- cord-005207-02wmt2e9 106 29 , , , cord-005207-02wmt2e9 106 30 Ministry Ministry NNP cord-005207-02wmt2e9 106 31 of of IN cord-005207-02wmt2e9 106 32 Science Science NNP cord-005207-02wmt2e9 106 33 , , , cord-005207-02wmt2e9 106 34 Korea Korea NNP cord-005207-02wmt2e9 106 35 . . .