id sid tid token lemma pos cord-269720-o81j3d1j 1 1 key key JJ cord-269720-o81j3d1j 1 2 : : : cord-269720-o81j3d1j 1 3 cord-269720-o81j3d1j cord-269720-o81j3d1j NN cord-269720-o81j3d1j 1 4 authors author NNS cord-269720-o81j3d1j 1 5 : : : cord-269720-o81j3d1j 1 6 Page Page NNP cord-269720-o81j3d1j 1 7 , , , cord-269720-o81j3d1j 1 8 Kevin Kevin NNP cord-269720-o81j3d1j 1 9 W. W. NNP cord-269720-o81j3d1j 1 10 ; ; : cord-269720-o81j3d1j 1 11 Britton Britton NNP cord-269720-o81j3d1j 1 12 , , , cord-269720-o81j3d1j 1 13 Paul Paul NNP cord-269720-o81j3d1j 1 14 ; ; : cord-269720-o81j3d1j 2 1 Boursnell Boursnell NNP cord-269720-o81j3d1j 2 2 , , , cord-269720-o81j3d1j 2 3 Michael Michael NNP cord-269720-o81j3d1j 2 4 E. E. NNP cord-269720-o81j3d1j 2 5 G. G. NNP cord-269720-o81j3d1j 3 1 title title NN cord-269720-o81j3d1j 3 2 : : : cord-269720-o81j3d1j 3 3 Sequence sequence NN cord-269720-o81j3d1j 3 4 analysis analysis NN cord-269720-o81j3d1j 3 5 of of IN cord-269720-o81j3d1j 3 6 the the DT cord-269720-o81j3d1j 3 7 leader leader NN cord-269720-o81j3d1j 3 8 RNA RNA NNP cord-269720-o81j3d1j 3 9 of of IN cord-269720-o81j3d1j 3 10 two two CD cord-269720-o81j3d1j 3 11 porcine porcine JJ cord-269720-o81j3d1j 3 12 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 3 13 : : : cord-269720-o81j3d1j 3 14 Transmissible transmissible JJ cord-269720-o81j3d1j 3 15 gastroenteritis gastroenteritis NN cord-269720-o81j3d1j 3 16 virus virus NN cord-269720-o81j3d1j 3 17 and and CC cord-269720-o81j3d1j 3 18 porcine porcine JJ cord-269720-o81j3d1j 3 19 respiratory respiratory JJ cord-269720-o81j3d1j 3 20 coronavirus coronavirus NN cord-269720-o81j3d1j 3 21 date date NN cord-269720-o81j3d1j 3 22 : : : cord-269720-o81j3d1j 3 23 1990 1990 CD cord-269720-o81j3d1j 3 24 journal journal NN cord-269720-o81j3d1j 3 25 : : : cord-269720-o81j3d1j 4 1 Virus Virus NNP cord-269720-o81j3d1j 4 2 Genes Genes NNP cord-269720-o81j3d1j 4 3 DOI DOI NNP cord-269720-o81j3d1j 4 4 : : : cord-269720-o81j3d1j 5 1 10.1007 10.1007 CD cord-269720-o81j3d1j 5 2 / / SYM cord-269720-o81j3d1j 5 3 bf00570024 bf00570024 NN cord-269720-o81j3d1j 5 4 sha sha NNP cord-269720-o81j3d1j 5 5 : : : cord-269720-o81j3d1j 5 6 4ef6ca984ee03204c7cfb6206fa85e63fc990507 4ef6ca984ee03204c7cfb6206fa85e63fc990507 CD cord-269720-o81j3d1j 6 1 doc_id doc_id CD cord-269720-o81j3d1j 6 2 : : : cord-269720-o81j3d1j 6 3 269720 269720 CD cord-269720-o81j3d1j 6 4 cord_uid cord_uid NNS cord-269720-o81j3d1j 6 5 : : : cord-269720-o81j3d1j 7 1 o81j3d1j o81j3d1j VBN cord-269720-o81j3d1j 8 1 The the DT cord-269720-o81j3d1j 8 2 leader leader NN cord-269720-o81j3d1j 8 3 RNA RNA NNP cord-269720-o81j3d1j 8 4 sequence sequence NN cord-269720-o81j3d1j 8 5 was be VBD cord-269720-o81j3d1j 8 6 determined determine VBN cord-269720-o81j3d1j 8 7 for for IN cord-269720-o81j3d1j 8 8 two two CD cord-269720-o81j3d1j 8 9 pig pig NN cord-269720-o81j3d1j 8 10 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 8 11 , , , cord-269720-o81j3d1j 8 12 tranmissible tranmissible JJ cord-269720-o81j3d1j 8 13 gastroenteritis gastroenteritis NN cord-269720-o81j3d1j 8 14 virus virus NN cord-269720-o81j3d1j 8 15 ( ( -LRB- cord-269720-o81j3d1j 8 16 TGEV TGEV NNP cord-269720-o81j3d1j 8 17 ) ) -RRB- cord-269720-o81j3d1j 8 18 , , , cord-269720-o81j3d1j 8 19 and and CC cord-269720-o81j3d1j 8 20 porcine porcine JJ cord-269720-o81j3d1j 8 21 respiratory respiratory JJ cord-269720-o81j3d1j 8 22 coronavirus coronavirus NN cord-269720-o81j3d1j 8 23 ( ( -LRB- cord-269720-o81j3d1j 8 24 PRCV PRCV NNP cord-269720-o81j3d1j 8 25 ) ) -RRB- cord-269720-o81j3d1j 8 26 . . . cord-269720-o81j3d1j 9 1 Primer primer NN cord-269720-o81j3d1j 9 2 extension extension NN cord-269720-o81j3d1j 9 3 , , , cord-269720-o81j3d1j 9 4 of of IN cord-269720-o81j3d1j 9 5 a a DT cord-269720-o81j3d1j 9 6 synthetic synthetic JJ cord-269720-o81j3d1j 9 7 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 9 8 complementary complementary JJ cord-269720-o81j3d1j 9 9 to to IN cord-269720-o81j3d1j 9 10 the the DT cord-269720-o81j3d1j 9 11 5′ 5′ CD cord-269720-o81j3d1j 9 12 end end NN cord-269720-o81j3d1j 9 13 of of IN cord-269720-o81j3d1j 9 14 the the DT cord-269720-o81j3d1j 9 15 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 9 16 gene gene NN cord-269720-o81j3d1j 9 17 of of IN cord-269720-o81j3d1j 9 18 TGEV TGEV NNP cord-269720-o81j3d1j 9 19 was be VBD cord-269720-o81j3d1j 9 20 used use VBN cord-269720-o81j3d1j 9 21 to to TO cord-269720-o81j3d1j 9 22 produce produce VB cord-269720-o81j3d1j 9 23 a a DT cord-269720-o81j3d1j 9 24 single single RB cord-269720-o81j3d1j 9 25 - - HYPH cord-269720-o81j3d1j 9 26 stranded strand VBN cord-269720-o81j3d1j 9 27 DNA dna NN cord-269720-o81j3d1j 9 28 copy copy NN cord-269720-o81j3d1j 9 29 of of IN cord-269720-o81j3d1j 9 30 the the DT cord-269720-o81j3d1j 9 31 leader leader NN cord-269720-o81j3d1j 9 32 RNA RNA NNP cord-269720-o81j3d1j 9 33 from from IN cord-269720-o81j3d1j 9 34 the the DT cord-269720-o81j3d1j 9 35 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 10 1 mRNA mrna NN cord-269720-o81j3d1j 10 2 species specie NNS cord-269720-o81j3d1j 10 3 from from IN cord-269720-o81j3d1j 10 4 TGEV TGEV NNP cord-269720-o81j3d1j 10 5 and and CC cord-269720-o81j3d1j 10 6 PRCV PRCV NNP cord-269720-o81j3d1j 10 7 , , , cord-269720-o81j3d1j 11 1 the the DT cord-269720-o81j3d1j 11 2 sequences sequence NNS cord-269720-o81j3d1j 11 3 of of IN cord-269720-o81j3d1j 11 4 which which WDT cord-269720-o81j3d1j 11 5 were be VBD cord-269720-o81j3d1j 11 6 determined determine VBN cord-269720-o81j3d1j 11 7 by by IN cord-269720-o81j3d1j 11 8 Maxam Maxam NNP cord-269720-o81j3d1j 11 9 and and CC cord-269720-o81j3d1j 11 10 Gilbert Gilbert NNP cord-269720-o81j3d1j 11 11 cleavage cleavage NN cord-269720-o81j3d1j 11 12 . . . cord-269720-o81j3d1j 12 1 Northern northern JJ cord-269720-o81j3d1j 12 2 blot blot NN cord-269720-o81j3d1j 12 3 analysis analysis NN cord-269720-o81j3d1j 12 4 , , , cord-269720-o81j3d1j 12 5 using use VBG cord-269720-o81j3d1j 12 6 a a DT cord-269720-o81j3d1j 12 7 synthetic synthetic JJ cord-269720-o81j3d1j 12 8 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 12 9 complementary complementary JJ cord-269720-o81j3d1j 12 10 to to IN cord-269720-o81j3d1j 12 11 the the DT cord-269720-o81j3d1j 12 12 leader leader NN cord-269720-o81j3d1j 12 13 RNA RNA NNP cord-269720-o81j3d1j 12 14 , , , cord-269720-o81j3d1j 12 15 showed show VBD cord-269720-o81j3d1j 12 16 that that IN cord-269720-o81j3d1j 12 17 the the DT cord-269720-o81j3d1j 12 18 leader leader NN cord-269720-o81j3d1j 12 19 RNA RNA NNP cord-269720-o81j3d1j 12 20 sequence sequence NN cord-269720-o81j3d1j 12 21 was be VBD cord-269720-o81j3d1j 12 22 present present JJ cord-269720-o81j3d1j 12 23 on on IN cord-269720-o81j3d1j 12 24 all all DT cord-269720-o81j3d1j 12 25 of of IN cord-269720-o81j3d1j 12 26 the the DT cord-269720-o81j3d1j 12 27 subgenomic subgenomic NNP cord-269720-o81j3d1j 12 28 mRNA mRNA NNP cord-269720-o81j3d1j 12 29 species specie NNS cord-269720-o81j3d1j 12 30 . . . cord-269720-o81j3d1j 13 1 The the DT cord-269720-o81j3d1j 13 2 porcine porcine JJ cord-269720-o81j3d1j 13 3 coronavirus coronavirus NN cord-269720-o81j3d1j 13 4 leader leader NN cord-269720-o81j3d1j 13 5 RNA RNA NNP cord-269720-o81j3d1j 13 6 sequences sequence NNS cord-269720-o81j3d1j 13 7 were be VBD cord-269720-o81j3d1j 13 8 compared compare VBN cord-269720-o81j3d1j 13 9 to to IN cord-269720-o81j3d1j 13 10 each each DT cord-269720-o81j3d1j 13 11 other other JJ cord-269720-o81j3d1j 13 12 and and CC cord-269720-o81j3d1j 13 13 to to IN cord-269720-o81j3d1j 13 14 published publish VBN cord-269720-o81j3d1j 13 15 coronavirus coronavirus NN cord-269720-o81j3d1j 13 16 leader leader NN cord-269720-o81j3d1j 13 17 RNA RNA NNP cord-269720-o81j3d1j 13 18 sequences sequence NNS cord-269720-o81j3d1j 13 19 . . . cord-269720-o81j3d1j 14 1 Sequence sequence NN cord-269720-o81j3d1j 14 2 homologies homology NNS cord-269720-o81j3d1j 14 3 and and CC cord-269720-o81j3d1j 14 4 secondary secondary JJ cord-269720-o81j3d1j 14 5 structure structure NN cord-269720-o81j3d1j 14 6 similarities similarity NNS cord-269720-o81j3d1j 14 7 were be VBD cord-269720-o81j3d1j 14 8 identified identify VBN cord-269720-o81j3d1j 14 9 that that WDT cord-269720-o81j3d1j 14 10 may may MD cord-269720-o81j3d1j 14 11 play play VB cord-269720-o81j3d1j 14 12 a a DT cord-269720-o81j3d1j 14 13 role role NN cord-269720-o81j3d1j 14 14 in in IN cord-269720-o81j3d1j 14 15 the the DT cord-269720-o81j3d1j 14 16 biological biological JJ cord-269720-o81j3d1j 14 17 function function NN cord-269720-o81j3d1j 14 18 of of IN cord-269720-o81j3d1j 14 19 these these DT cord-269720-o81j3d1j 14 20 RNA RNA NNP cord-269720-o81j3d1j 14 21 sequences sequence NNS cord-269720-o81j3d1j 14 22 . . . cord-269720-o81j3d1j 15 1 Transmissible transmissible JJ cord-269720-o81j3d1j 15 2 gastroenteritis gastroenteritis NN cord-269720-o81j3d1j 15 3 virus virus NN cord-269720-o81j3d1j 15 4 ( ( -LRB- cord-269720-o81j3d1j 15 5 TGEV TGEV NNP cord-269720-o81j3d1j 15 6 ) ) -RRB- cord-269720-o81j3d1j 15 7 and and CC cord-269720-o81j3d1j 15 8 porcine porcine JJ cord-269720-o81j3d1j 15 9 respiratory respiratory JJ cord-269720-o81j3d1j 15 10 coronavirus coronavirus NN cord-269720-o81j3d1j 15 11 ( ( -LRB- cord-269720-o81j3d1j 15 12 PRCV PRCV NNP cord-269720-o81j3d1j 15 13 ) ) -RRB- cord-269720-o81j3d1j 15 14 belong belong VBP cord-269720-o81j3d1j 15 15 to to IN cord-269720-o81j3d1j 15 16 the the DT cord-269720-o81j3d1j 15 17 family family NN cord-269720-o81j3d1j 15 18 Coronaviridae Coronaviridae NNP cord-269720-o81j3d1j 15 19 , , , cord-269720-o81j3d1j 15 20 a a DT cord-269720-o81j3d1j 15 21 large large JJ cord-269720-o81j3d1j 15 22 group group NN cord-269720-o81j3d1j 15 23 of of IN cord-269720-o81j3d1j 15 24 pleomorphic pleomorphic JJ cord-269720-o81j3d1j 15 25 enveloped envelop VBN cord-269720-o81j3d1j 15 26 viruses virus NNS cord-269720-o81j3d1j 15 27 with with IN cord-269720-o81j3d1j 15 28 a a DT cord-269720-o81j3d1j 15 29 positive positive JJ cord-269720-o81j3d1j 15 30 - - HYPH cord-269720-o81j3d1j 15 31 stranded strand VBN cord-269720-o81j3d1j 15 32 RNA RNA NNP cord-269720-o81j3d1j 15 33 genome genome NN cord-269720-o81j3d1j 15 34 . . . cord-269720-o81j3d1j 16 1 TGEV TGEV NNP cord-269720-o81j3d1j 16 2 causes cause VBZ cord-269720-o81j3d1j 16 3 gastroenteritis gastroenteritis NN cord-269720-o81j3d1j 16 4 in in IN cord-269720-o81j3d1j 16 5 pigs pig NNS cord-269720-o81j3d1j 16 6 , , , cord-269720-o81j3d1j 16 7 resulting result VBG cord-269720-o81j3d1j 16 8 in in IN cord-269720-o81j3d1j 16 9 a a DT cord-269720-o81j3d1j 16 10 high high JJ cord-269720-o81j3d1j 16 11 mortality mortality NN cord-269720-o81j3d1j 16 12 in in IN cord-269720-o81j3d1j 16 13 neonates neonate NNS cord-269720-o81j3d1j 16 14 ( ( -LRB- cord-269720-o81j3d1j 16 15 1 1 CD cord-269720-o81j3d1j 16 16 ) ) -RRB- cord-269720-o81j3d1j 16 17 . . . cord-269720-o81j3d1j 17 1 PRCV PRCV NNP cord-269720-o81j3d1j 17 2 was be VBD cord-269720-o81j3d1j 17 3 isolated isolate VBN cord-269720-o81j3d1j 17 4 in in IN cord-269720-o81j3d1j 17 5 several several JJ cord-269720-o81j3d1j 17 6 European european JJ cord-269720-o81j3d1j 17 7 countries country NNS cord-269720-o81j3d1j 17 8 between between IN cord-269720-o81j3d1j 17 9 1984 1984 CD cord-269720-o81j3d1j 17 10 and and CC cord-269720-o81j3d1j 17 11 1986 1986 CD cord-269720-o81j3d1j 17 12 ( ( -LRB- cord-269720-o81j3d1j 17 13 2 2 CD cord-269720-o81j3d1j 17 14 - - SYM cord-269720-o81j3d1j 17 15 4 4 CD cord-269720-o81j3d1j 17 16 ) ) -RRB- cord-269720-o81j3d1j 17 17 , , , cord-269720-o81j3d1j 17 18 does do VBZ cord-269720-o81j3d1j 17 19 not not RB cord-269720-o81j3d1j 17 20 cause cause VB cord-269720-o81j3d1j 17 21 diarrhea diarrhea NN cord-269720-o81j3d1j 17 22 , , , cord-269720-o81j3d1j 17 23 and and CC cord-269720-o81j3d1j 17 24 has have VBZ cord-269720-o81j3d1j 17 25 been be VBN cord-269720-o81j3d1j 17 26 shown show VBN cord-269720-o81j3d1j 17 27 to to TO cord-269720-o81j3d1j 17 28 replicate replicate VB cord-269720-o81j3d1j 17 29 in in IN cord-269720-o81j3d1j 17 30 the the DT cord-269720-o81j3d1j 17 31 respiratory respiratory JJ cord-269720-o81j3d1j 17 32 tract tract NN cord-269720-o81j3d1j 17 33 with with IN cord-269720-o81j3d1j 17 34 little little JJ cord-269720-o81j3d1j 17 35 or or CC cord-269720-o81j3d1j 17 36 no no DT cord-269720-o81j3d1j 17 37 clinical clinical JJ cord-269720-o81j3d1j 17 38 signs sign NNS cord-269720-o81j3d1j 17 39 , , , cord-269720-o81j3d1j 17 40 but but CC cord-269720-o81j3d1j 17 41 is be VBZ cord-269720-o81j3d1j 17 42 very very RB cord-269720-o81j3d1j 17 43 similar similar JJ cord-269720-o81j3d1j 17 44 antigenically antigenically NN cord-269720-o81j3d1j 17 45 and and CC cord-269720-o81j3d1j 17 46 serologically serologically RB cord-269720-o81j3d1j 17 47 to to IN cord-269720-o81j3d1j 17 48 TGEV TGEV NNP cord-269720-o81j3d1j 17 49 ( ( -LRB- cord-269720-o81j3d1j 17 50 2 2 CD cord-269720-o81j3d1j 17 51 , , , cord-269720-o81j3d1j 17 52 4 4 CD cord-269720-o81j3d1j 17 53 ) ) -RRB- cord-269720-o81j3d1j 17 54 . . . cord-269720-o81j3d1j 18 1 Virions virion NNS cord-269720-o81j3d1j 18 2 from from IN cord-269720-o81j3d1j 18 3 both both DT cord-269720-o81j3d1j 18 4 viruses virus NNS cord-269720-o81j3d1j 18 5 contain contain VBP cord-269720-o81j3d1j 18 6 two two CD cord-269720-o81j3d1j 18 7 envelope envelope NN cord-269720-o81j3d1j 18 8 glycoproteins glycoprotein NNS cord-269720-o81j3d1j 18 9 of of IN cord-269720-o81j3d1j 18 10 relative relative JJ cord-269720-o81j3d1j 18 11 molecular molecular JJ cord-269720-o81j3d1j 18 12 mass mass NN cord-269720-o81j3d1j 18 13 ( ( -LRB- cord-269720-o81j3d1j 18 14 Mr Mr NNP cord-269720-o81j3d1j 18 15 ) ) -RRB- cord-269720-o81j3d1j 18 16 200,000 200,000 CD cord-269720-o81j3d1j 18 17 ( ( -LRB- cord-269720-o81j3d1j 18 18 spike spike NN cord-269720-o81j3d1j 18 19 ) ) -RRB- cord-269720-o81j3d1j 18 20 and and CC cord-269720-o81j3d1j 18 21 M M NNP cord-269720-o81j3d1j 18 22 r r NN cord-269720-o81j3d1j 18 23 28,000 28,000 CD cord-269720-o81j3d1j 18 24 - - HYPH cord-269720-o81j3d1j 18 25 31,000 31,000 CD cord-269720-o81j3d1j 18 26 ( ( -LRB- cord-269720-o81j3d1j 18 27 membrane membrane NN cord-269720-o81j3d1j 18 28 protein protein NN cord-269720-o81j3d1j 18 29 ) ) -RRB- cord-269720-o81j3d1j 18 30 and and CC cord-269720-o81j3d1j 18 31 a a DT cord-269720-o81j3d1j 18 32 phosphorylated phosphorylated JJ cord-269720-o81j3d1j 18 33 nucleoprotein nucleoprotein NN cord-269720-o81j3d1j 18 34 of of IN cord-269720-o81j3d1j 18 35 M M NNP cord-269720-o81j3d1j 18 36 r r NNP cord-269720-o81j3d1j 18 37 47,000 47,000 CD cord-269720-o81j3d1j 18 38 . . . cord-269720-o81j3d1j 18 39 cDNA cdna NN cord-269720-o81j3d1j 18 40 probes probe NNS cord-269720-o81j3d1j 18 41 to to IN cord-269720-o81j3d1j 18 42 the the DT cord-269720-o81j3d1j 18 43 structural structural JJ cord-269720-o81j3d1j 18 44 protein protein NN cord-269720-o81j3d1j 18 45 genes gene NNS cord-269720-o81j3d1j 18 46 of of IN cord-269720-o81j3d1j 18 47 TGEV TGEV NNP cord-269720-o81j3d1j 18 48 hybridized hybridize VBN cord-269720-o81j3d1j 18 49 to to IN cord-269720-o81j3d1j 18 50 the the DT cord-269720-o81j3d1j 18 51 appropriate appropriate JJ cord-269720-o81j3d1j 18 52 mRNA mRNA NNP cord-269720-o81j3d1j 18 53 species specie NNS cord-269720-o81j3d1j 18 54 of of IN cord-269720-o81j3d1j 18 55 PRCV PRCV NNP cord-269720-o81j3d1j 18 56 , , , cord-269720-o81j3d1j 18 57 suggesting suggest VBG cord-269720-o81j3d1j 18 58 a a DT cord-269720-o81j3d1j 18 59 high high JJ cord-269720-o81j3d1j 18 60 degree degree NN cord-269720-o81j3d1j 18 61 of of IN cord-269720-o81j3d1j 18 62 homology homology NN cord-269720-o81j3d1j 18 63 at at IN cord-269720-o81j3d1j 18 64 the the DT cord-269720-o81j3d1j 18 65 RNA RNA NNP cord-269720-o81j3d1j 18 66 level level NN cord-269720-o81j3d1j 18 67 ( ( -LRB- cord-269720-o81j3d1j 18 68 unpublished unpublished JJ cord-269720-o81j3d1j 18 69 data datum NNS cord-269720-o81j3d1j 18 70 ) ) -RRB- cord-269720-o81j3d1j 18 71 . . . cord-269720-o81j3d1j 19 1 Coronavirus Coronavirus NNP cord-269720-o81j3d1j 19 2 proteins protein NNS cord-269720-o81j3d1j 19 3 are be VBP cord-269720-o81j3d1j 19 4 expressed express VBN cord-269720-o81j3d1j 19 5 from from IN cord-269720-o81j3d1j 19 6 a a DT cord-269720-o81j3d1j 19 7 " " `` cord-269720-o81j3d1j 19 8 nested nested JJ cord-269720-o81j3d1j 19 9 " " '' cord-269720-o81j3d1j 19 10 set set NN cord-269720-o81j3d1j 19 11 of of IN cord-269720-o81j3d1j 19 12 subgenomic subgenomic NNP cord-269720-o81j3d1j 19 13 mRNAs mRNAs NNPS cord-269720-o81j3d1j 19 14 with with IN cord-269720-o81j3d1j 19 15 common common JJ cord-269720-o81j3d1j 19 16 3 3 CD cord-269720-o81j3d1j 19 17 ' ' NN cord-269720-o81j3d1j 19 18 termini terminus NNS cord-269720-o81j3d1j 19 19 but but CC cord-269720-o81j3d1j 19 20 different different JJ cord-269720-o81j3d1j 19 21 5 5 CD cord-269720-o81j3d1j 19 22 ' ' POS cord-269720-o81j3d1j 19 23 extensions extension NNS cord-269720-o81j3d1j 19 24 . . . cord-269720-o81j3d1j 20 1 The the DT cord-269720-o81j3d1j 20 2 sequence sequence NN cord-269720-o81j3d1j 20 3 of of IN cord-269720-o81j3d1j 20 4 each each DT cord-269720-o81j3d1j 20 5 mRNA mrna NN cord-269720-o81j3d1j 20 6 that that WDT cord-269720-o81j3d1j 20 7 is be VBZ cord-269720-o81j3d1j 20 8 translated translate VBN cord-269720-o81j3d1j 20 9 to to TO cord-269720-o81j3d1j 20 10 produce produce VB cord-269720-o81j3d1j 20 11 viral viral JJ cord-269720-o81j3d1j 20 12 proteins protein NNS cord-269720-o81j3d1j 20 13 appears appear VBZ cord-269720-o81j3d1j 20 14 to to TO cord-269720-o81j3d1j 20 15 correspond correspond VB cord-269720-o81j3d1j 20 16 to to IN cord-269720-o81j3d1j 20 17 the the DT cord-269720-o81j3d1j 20 18 5'-terminal 5'-terminal CD cord-269720-o81j3d1j 20 19 region region NN cord-269720-o81j3d1j 20 20 that that WDT cord-269720-o81j3d1j 20 21 is be VBZ cord-269720-o81j3d1j 20 22 absent absent JJ cord-269720-o81j3d1j 20 23 on on IN cord-269720-o81j3d1j 20 24 the the DT cord-269720-o81j3d1j 20 25 preceding precede VBG cord-269720-o81j3d1j 20 26 smaller small JJR cord-269720-o81j3d1j 20 27 mRNA mRNA NNP cord-269720-o81j3d1j 20 28 species specie NNS cord-269720-o81j3d1j 20 29 . . . cord-269720-o81j3d1j 21 1 It -PRON- PRP cord-269720-o81j3d1j 21 2 has have VBZ cord-269720-o81j3d1j 21 3 been be VBN cord-269720-o81j3d1j 21 4 shown show VBN cord-269720-o81j3d1j 21 5 for for IN cord-269720-o81j3d1j 21 6 the the DT cord-269720-o81j3d1j 21 7 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 21 8 , , , cord-269720-o81j3d1j 21 9 mouse mouse NNP cord-269720-o81j3d1j 21 10 hepatitis hepatitis NN cord-269720-o81j3d1j 21 11 virus virus NN cord-269720-o81j3d1j 21 12 ( ( -LRB- cord-269720-o81j3d1j 21 13 MHV MHV NNP cord-269720-o81j3d1j 21 14 ) ) -RRB- cord-269720-o81j3d1j 21 15 and and CC cord-269720-o81j3d1j 21 16 infectious infectious JJ cord-269720-o81j3d1j 21 17 bronchitis bronchitis NN cord-269720-o81j3d1j 21 18 virus virus NN cord-269720-o81j3d1j 21 19 ( ( -LRB- cord-269720-o81j3d1j 21 20 IBV IBV NNP cord-269720-o81j3d1j 21 21 ) ) -RRB- cord-269720-o81j3d1j 21 22 , , , cord-269720-o81j3d1j 21 23 the the DT cord-269720-o81j3d1j 21 24 subgenomic subgenomic NNP cord-269720-o81j3d1j 21 25 mRNA mRNA NNP cord-269720-o81j3d1j 21 26 species specie NNS cord-269720-o81j3d1j 21 27 possess possess VBP cord-269720-o81j3d1j 21 28 short short JJ cord-269720-o81j3d1j 21 29 " " `` cord-269720-o81j3d1j 21 30 leader leader NN cord-269720-o81j3d1j 21 31 sequences sequence NNS cord-269720-o81j3d1j 21 32 " " '' cord-269720-o81j3d1j 21 33 at at IN cord-269720-o81j3d1j 21 34 their -PRON- PRP$ cord-269720-o81j3d1j 21 35 5 5 CD cord-269720-o81j3d1j 21 36 ' ' '' cord-269720-o81j3d1j 21 37 ends end NNS cord-269720-o81j3d1j 21 38 . . . cord-269720-o81j3d1j 22 1 These these DT cord-269720-o81j3d1j 22 2 sequences sequence NNS cord-269720-o81j3d1j 22 3 are be VBP cord-269720-o81j3d1j 22 4 not not RB cord-269720-o81j3d1j 22 5 transcribed transcribe VBN cord-269720-o81j3d1j 22 6 as as IN cord-269720-o81j3d1j 22 7 a a DT cord-269720-o81j3d1j 22 8 contiguous contiguous JJ cord-269720-o81j3d1j 22 9 mRNA mRNA NNP cord-269720-o81j3d1j 22 10 species specie NNS cord-269720-o81j3d1j 22 11 , , , cord-269720-o81j3d1j 22 12 but but CC cord-269720-o81j3d1j 22 13 are be VBP cord-269720-o81j3d1j 22 14 derived derive VBN cord-269720-o81j3d1j 22 15 from from IN cord-269720-o81j3d1j 22 16 the the DT cord-269720-o81j3d1j 22 17 5 5 CD cord-269720-o81j3d1j 22 18 ' ' NN cord-269720-o81j3d1j 22 19 end end NN cord-269720-o81j3d1j 22 20 of of IN cord-269720-o81j3d1j 22 21 the the DT cord-269720-o81j3d1j 22 22 genomic genomic JJ cord-269720-o81j3d1j 22 23 RNA RNA NNP cord-269720-o81j3d1j 22 24 and and CC cord-269720-o81j3d1j 22 25 are be VBP cord-269720-o81j3d1j 22 26 probably probably RB cord-269720-o81j3d1j 22 27 joined join VBN cord-269720-o81j3d1j 22 28 to to IN cord-269720-o81j3d1j 22 29 the the DT cord-269720-o81j3d1j 22 30 5 5 CD cord-269720-o81j3d1j 22 31 ' ' NN cord-269720-o81j3d1j 22 32 end end NN cord-269720-o81j3d1j 22 33 of of IN cord-269720-o81j3d1j 22 34 each each DT cord-269720-o81j3d1j 22 35 mRNA mrna NN cord-269720-o81j3d1j 22 36 by by IN cord-269720-o81j3d1j 22 37 a a DT cord-269720-o81j3d1j 22 38 process process NN cord-269720-o81j3d1j 22 39 of of IN cord-269720-o81j3d1j 22 40 discontinuous discontinuous JJ cord-269720-o81j3d1j 22 41 transcription transcription NN cord-269720-o81j3d1j 22 42 ( ( -LRB- cord-269720-o81j3d1j 22 43 5 5 CD cord-269720-o81j3d1j 22 44 ) ) -RRB- cord-269720-o81j3d1j 23 1 ( ( -LRB- cord-269720-o81j3d1j 23 2 6 6 LS cord-269720-o81j3d1j 23 3 ) ) -RRB- cord-269720-o81j3d1j 23 4 ( ( -LRB- cord-269720-o81j3d1j 23 5 7 7 LS cord-269720-o81j3d1j 23 6 ) ) -RRB- cord-269720-o81j3d1j 23 7 ( ( -LRB- cord-269720-o81j3d1j 23 8 8) 8) NNP cord-269720-o81j3d1j 23 9 ( ( -LRB- cord-269720-o81j3d1j 23 10 9 9 CD cord-269720-o81j3d1j 23 11 ) ) -RRB- cord-269720-o81j3d1j 23 12 . . . cord-269720-o81j3d1j 24 1 The the DT cord-269720-o81j3d1j 24 2 leader leader NN cord-269720-o81j3d1j 24 3 sequence sequence NN cord-269720-o81j3d1j 24 4 appears appear VBZ cord-269720-o81j3d1j 24 5 to to TO cord-269720-o81j3d1j 24 6 be be VB cord-269720-o81j3d1j 24 7 produced produce VBN cord-269720-o81j3d1j 24 8 by by IN cord-269720-o81j3d1j 24 9 a a DT cord-269720-o81j3d1j 24 10 mechanism mechanism NN cord-269720-o81j3d1j 24 11 termed term VBN cord-269720-o81j3d1j 24 12 leader leader NN cord-269720-o81j3d1j 24 13 - - HYPH cord-269720-o81j3d1j 24 14 primed prime VBN cord-269720-o81j3d1j 24 15 transcription transcription NN cord-269720-o81j3d1j 24 16 , , , cord-269720-o81j3d1j 24 17 in in IN cord-269720-o81j3d1j 24 18 which which WDT cord-269720-o81j3d1j 24 19 the the DT cord-269720-o81j3d1j 24 20 leader leader NN cord-269720-o81j3d1j 24 21 RNA RNA NNP cord-269720-o81j3d1j 24 22 is be VBZ cord-269720-o81j3d1j 24 23 transcribed transcribe VBN cord-269720-o81j3d1j 24 24 independently independently RB cord-269720-o81j3d1j 24 25 , , , cord-269720-o81j3d1j 24 26 dissociated dissociate VBN cord-269720-o81j3d1j 24 27 from from IN cord-269720-o81j3d1j 24 28 the the DT cord-269720-o81j3d1j 24 29 template template NN cord-269720-o81j3d1j 24 30 , , , cord-269720-o81j3d1j 24 31 and and CC cord-269720-o81j3d1j 24 32 then then RB cord-269720-o81j3d1j 24 33 binds bind VBZ cord-269720-o81j3d1j 24 34 to to IN cord-269720-o81j3d1j 24 35 the the DT cord-269720-o81j3d1j 24 36 template template NN cord-269720-o81j3d1j 24 37 ( ( -LRB- cord-269720-o81j3d1j 24 38 negative negative JJ cord-269720-o81j3d1j 24 39 - - HYPH cord-269720-o81j3d1j 24 40 sense sense NN cord-269720-o81j3d1j 24 41 strand strand NN cord-269720-o81j3d1j 24 42 ) ) -RRB- cord-269720-o81j3d1j 24 43 at at IN cord-269720-o81j3d1j 24 44 specific specific JJ cord-269720-o81j3d1j 24 45 transcriptional transcriptional JJ cord-269720-o81j3d1j 24 46 start start NN cord-269720-o81j3d1j 24 47 sites site NNS cord-269720-o81j3d1j 24 48 ( ( -LRB- cord-269720-o81j3d1j 24 49 I0 i0 NN cord-269720-o81j3d1j 24 50 , , , cord-269720-o81j3d1j 24 51 11 11 CD cord-269720-o81j3d1j 24 52 ) ) -RRB- cord-269720-o81j3d1j 24 53 . . . cord-269720-o81j3d1j 25 1 The the DT cord-269720-o81j3d1j 25 2 mechanism mechanism NN cord-269720-o81j3d1j 25 3 appears appear VBZ cord-269720-o81j3d1j 25 4 to to TO cord-269720-o81j3d1j 25 5 involve involve VB cord-269720-o81j3d1j 25 6 the the DT cord-269720-o81j3d1j 25 7 recognition recognition NN cord-269720-o81j3d1j 25 8 of of IN cord-269720-o81j3d1j 25 9 consensus consensus NN cord-269720-o81j3d1j 25 10 sequences sequence NNS cord-269720-o81j3d1j 25 11 identified identify VBN cord-269720-o81j3d1j 25 12 on on IN cord-269720-o81j3d1j 25 13 the the DT cord-269720-o81j3d1j 25 14 genomic genomic JJ cord-269720-o81j3d1j 25 15 RNA rna NN cord-269720-o81j3d1j 25 16 at at IN cord-269720-o81j3d1j 25 17 those those DT cord-269720-o81j3d1j 25 18 points point NNS cord-269720-o81j3d1j 25 19 corresponding correspond VBG cord-269720-o81j3d1j 25 20 to to IN cord-269720-o81j3d1j 25 21 the the DT cord-269720-o81j3d1j 25 22 5 5 CD cord-269720-o81j3d1j 25 23 ' ' '' cord-269720-o81j3d1j 25 24 ends end NNS cord-269720-o81j3d1j 25 25 of of IN cord-269720-o81j3d1j 25 26 the the DT cord-269720-o81j3d1j 25 27 subgenomic subgenomic NNP cord-269720-o81j3d1j 25 28 mRNAs mRNAs NNPS cord-269720-o81j3d1j 25 29 . . . cord-269720-o81j3d1j 26 1 These these DT cord-269720-o81j3d1j 26 2 consensus consensus NN cord-269720-o81j3d1j 26 3 sequences sequence NNS cord-269720-o81j3d1j 26 4 may may MD cord-269720-o81j3d1j 26 5 act act VB cord-269720-o81j3d1j 26 6 as as IN cord-269720-o81j3d1j 26 7 a a DT cord-269720-o81j3d1j 26 8 binding binding NN cord-269720-o81j3d1j 26 9 site site NN cord-269720-o81j3d1j 26 10 for for IN cord-269720-o81j3d1j 26 11 the the DT cord-269720-o81j3d1j 26 12 RNA RNA NNP cord-269720-o81j3d1j 26 13 polymeraseleader polymeraseleader NN cord-269720-o81j3d1j 26 14 complex complex NN cord-269720-o81j3d1j 26 15 ( ( -LRB- cord-269720-o81j3d1j 26 16 7 7 CD cord-269720-o81j3d1j 26 17 ) ) -RRB- cord-269720-o81j3d1j 26 18 ( ( -LRB- cord-269720-o81j3d1j 26 19 8) 8) NNP cord-269720-o81j3d1j 26 20 ( ( -LRB- cord-269720-o81j3d1j 26 21 9 9 CD cord-269720-o81j3d1j 26 22 ) ) -RRB- cord-269720-o81j3d1j 26 23 ( ( -LRB- cord-269720-o81j3d1j 26 24 12 12 CD cord-269720-o81j3d1j 26 25 ) ) -RRB- cord-269720-o81j3d1j 26 26 ( ( -LRB- cord-269720-o81j3d1j 26 27 13 13 CD cord-269720-o81j3d1j 26 28 ) ) -RRB- cord-269720-o81j3d1j 26 29 ( ( -LRB- cord-269720-o81j3d1j 26 30 14 14 CD cord-269720-o81j3d1j 26 31 ) ) -RRB- cord-269720-o81j3d1j 26 32 . . . cord-269720-o81j3d1j 27 1 It -PRON- PRP cord-269720-o81j3d1j 27 2 has have VBZ cord-269720-o81j3d1j 27 3 been be VBN cord-269720-o81j3d1j 27 4 previously previously RB cord-269720-o81j3d1j 27 5 postulated postulate VBN cord-269720-o81j3d1j 27 6 that that IN cord-269720-o81j3d1j 27 7 a a DT cord-269720-o81j3d1j 27 8 heptameric heptameric JJ cord-269720-o81j3d1j 27 9 sequence sequence NN cord-269720-o81j3d1j 27 10 , , , cord-269720-o81j3d1j 27 11 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 27 12 ( ( -LRB- cord-269720-o81j3d1j 27 13 15 15 CD cord-269720-o81j3d1j 27 14 ) ) -RRB- cord-269720-o81j3d1j 27 15 ( ( -LRB- cord-269720-o81j3d1j 27 16 16 16 CD cord-269720-o81j3d1j 27 17 ) ) -RRB- cord-269720-o81j3d1j 27 18 ( ( -LRB- cord-269720-o81j3d1j 27 19 17 17 CD cord-269720-o81j3d1j 27 20 ) ) -RRB- cord-269720-o81j3d1j 27 21 , , , cord-269720-o81j3d1j 27 22 or or CC cord-269720-o81j3d1j 27 23 a a DT cord-269720-o81j3d1j 27 24 hexameric hexameric JJ cord-269720-o81j3d1j 27 25 sequence sequence NN cord-269720-o81j3d1j 27 26 , , , cord-269720-o81j3d1j 27 27 CTAAAC CTAAAC NNP cord-269720-o81j3d1j 27 28 ( ( -LRB- cord-269720-o81j3d1j 27 29 18 18 CD cord-269720-o81j3d1j 27 30 ) ) -RRB- cord-269720-o81j3d1j 27 31 ( ( -LRB- cord-269720-o81j3d1j 27 32 19 19 CD cord-269720-o81j3d1j 27 33 ) ) -RRB- cord-269720-o81j3d1j 27 34 ( ( -LRB- cord-269720-o81j3d1j 27 35 20 20 CD cord-269720-o81j3d1j 27 36 ) ) -RRB- cord-269720-o81j3d1j 27 37 , , , cord-269720-o81j3d1j 27 38 may may MD cord-269720-o81j3d1j 27 39 be be VB cord-269720-o81j3d1j 27 40 involved involve VBN cord-269720-o81j3d1j 27 41 in in IN cord-269720-o81j3d1j 27 42 the the DT cord-269720-o81j3d1j 27 43 binding binding NN cord-269720-o81j3d1j 27 44 of of IN cord-269720-o81j3d1j 27 45 the the DT cord-269720-o81j3d1j 27 46 TGEV TGEV NNP cord-269720-o81j3d1j 27 47 RNA RNA NNP cord-269720-o81j3d1j 27 48 polymerase polymerase NN cord-269720-o81j3d1j 27 49 leader leader NN cord-269720-o81j3d1j 27 50 . . . cord-269720-o81j3d1j 28 1 In in IN cord-269720-o81j3d1j 28 2 this this DT cord-269720-o81j3d1j 28 3 paper paper NN cord-269720-o81j3d1j 28 4 we -PRON- PRP cord-269720-o81j3d1j 28 5 describe describe VBP cord-269720-o81j3d1j 28 6 the the DT cord-269720-o81j3d1j 28 7 elucidation elucidation NN cord-269720-o81j3d1j 28 8 of of IN cord-269720-o81j3d1j 28 9 the the DT cord-269720-o81j3d1j 28 10 leader leader NN cord-269720-o81j3d1j 28 11 RNA RNA NNP cord-269720-o81j3d1j 28 12 sequences sequence NNS cord-269720-o81j3d1j 28 13 from from IN cord-269720-o81j3d1j 28 14 the the DT cord-269720-o81j3d1j 28 15 porcine porcine JJ cord-269720-o81j3d1j 28 16 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 28 17 TGEV TGEV NNP cord-269720-o81j3d1j 28 18 and and CC cord-269720-o81j3d1j 28 19 PRCV PRCV NNP cord-269720-o81j3d1j 28 20 , , , cord-269720-o81j3d1j 28 21 the the DT cord-269720-o81j3d1j 28 22 first first JJ cord-269720-o81j3d1j 28 23 leader leader NN cord-269720-o81j3d1j 28 24 sequence sequence NN cord-269720-o81j3d1j 28 25 to to TO cord-269720-o81j3d1j 28 26 be be VB cord-269720-o81j3d1j 28 27 described describe VBN cord-269720-o81j3d1j 28 28 from from IN cord-269720-o81j3d1j 28 29 the the DT cord-269720-o81j3d1j 28 30 TGEV TGEV NNP cord-269720-o81j3d1j 28 31 serogroup serogroup NN cord-269720-o81j3d1j 28 32 of of IN cord-269720-o81j3d1j 28 33 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 28 34 . . . cord-269720-o81j3d1j 29 1 Comparison comparison NN cord-269720-o81j3d1j 29 2 of of IN cord-269720-o81j3d1j 29 3 the the DT cord-269720-o81j3d1j 29 4 leader leader NN cord-269720-o81j3d1j 29 5 RNAs rna NNS cord-269720-o81j3d1j 29 6 of of IN cord-269720-o81j3d1j 29 7 TGEV TGEV NNP cord-269720-o81j3d1j 29 8 and and CC cord-269720-o81j3d1j 29 9 PRCV PRCV NNP cord-269720-o81j3d1j 29 10 with with IN cord-269720-o81j3d1j 29 11 published publish VBN cord-269720-o81j3d1j 29 12 leader leader NN cord-269720-o81j3d1j 29 13 RNAs rna NNS cord-269720-o81j3d1j 29 14 of of IN cord-269720-o81j3d1j 29 15 other other JJ cord-269720-o81j3d1j 29 16 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 29 17 was be VBD cord-269720-o81j3d1j 29 18 used use VBN cord-269720-o81j3d1j 29 19 to to TO cord-269720-o81j3d1j 29 20 identify identify VB cord-269720-o81j3d1j 29 21 areas area NNS cord-269720-o81j3d1j 29 22 of of IN cord-269720-o81j3d1j 29 23 conserved conserved JJ cord-269720-o81j3d1j 29 24 sequence sequence NN cord-269720-o81j3d1j 29 25 and and CC cord-269720-o81j3d1j 29 26 potential potential JJ cord-269720-o81j3d1j 29 27 secondary secondary JJ cord-269720-o81j3d1j 29 28 structure structure NN cord-269720-o81j3d1j 29 29 that that WDT cord-269720-o81j3d1j 29 30 may may MD cord-269720-o81j3d1j 29 31 be be VB cord-269720-o81j3d1j 29 32 involved involve VBN cord-269720-o81j3d1j 29 33 in in IN cord-269720-o81j3d1j 29 34 the the DT cord-269720-o81j3d1j 29 35 transcription transcription NN cord-269720-o81j3d1j 29 36 of of IN cord-269720-o81j3d1j 29 37 coronavirus coronavirus NN cord-269720-o81j3d1j 29 38 subgenomic subgenomic NNP cord-269720-o81j3d1j 29 39 mRNA mRNA NNP cord-269720-o81j3d1j 29 40 species specie NNS cord-269720-o81j3d1j 29 41 . . . cord-269720-o81j3d1j 30 1 Confluent confluent NN cord-269720-o81j3d1j 30 2 cultures culture NNS cord-269720-o81j3d1j 30 3 of of IN cord-269720-o81j3d1j 30 4 a a DT cord-269720-o81j3d1j 30 5 pig pig NN cord-269720-o81j3d1j 30 6 kidney kidney NN cord-269720-o81j3d1j 30 7 cell cell NN cord-269720-o81j3d1j 30 8 line line NN cord-269720-o81j3d1j 30 9 LLC LLC NNP cord-269720-o81j3d1j 30 10 - - HYPH cord-269720-o81j3d1j 30 11 PK1 PK1 NNP cord-269720-o81j3d1j 30 12 were be VBD cord-269720-o81j3d1j 30 13 infected infect VBN cord-269720-o81j3d1j 30 14 with with IN cord-269720-o81j3d1j 30 15 a a DT cord-269720-o81j3d1j 30 16 virulent virulent JJ cord-269720-o81j3d1j 30 17 British british JJ cord-269720-o81j3d1j 30 18 field field NN cord-269720-o81j3d1j 30 19 isolate isolate VBP cord-269720-o81j3d1j 30 20 of of IN cord-269720-o81j3d1j 30 21 TGEV TGEV NNP cord-269720-o81j3d1j 30 22 strain strain NN cord-269720-o81j3d1j 30 23 FS772/70 FS772/70 NNP cord-269720-o81j3d1j 30 24 or or CC cord-269720-o81j3d1j 30 25 a a DT cord-269720-o81j3d1j 30 26 British british JJ cord-269720-o81j3d1j 30 27 isolate isolate NN cord-269720-o81j3d1j 30 28 of of IN cord-269720-o81j3d1j 30 29 PRCV PRCV NNP cord-269720-o81j3d1j 31 1 strain strain VB cord-269720-o81j3d1j 31 2 86/137004 86/137004 CD cord-269720-o81j3d1j 31 3 at at IN cord-269720-o81j3d1j 31 4 a a DT cord-269720-o81j3d1j 31 5 MOI MOI NNP cord-269720-o81j3d1j 31 6 of of IN cord-269720-o81j3d1j 31 7 1 1 CD cord-269720-o81j3d1j 31 8 - - SYM cord-269720-o81j3d1j 31 9 10 10 CD cord-269720-o81j3d1j 31 10 PFU pfu NN cord-269720-o81j3d1j 31 11 per per IN cord-269720-o81j3d1j 31 12 cell cell NN cord-269720-o81j3d1j 31 13 . . . cord-269720-o81j3d1j 32 1 After after IN cord-269720-o81j3d1j 32 2 2 2 CD cord-269720-o81j3d1j 32 3 hr hr NN cord-269720-o81j3d1j 32 4 at at IN cord-269720-o81j3d1j 32 5 37~ 37~ CD cord-269720-o81j3d1j 32 6 the the DT cord-269720-o81j3d1j 32 7 inoculum inoculum NN cord-269720-o81j3d1j 32 8 was be VBD cord-269720-o81j3d1j 32 9 removed remove VBN cord-269720-o81j3d1j 32 10 and and CC cord-269720-o81j3d1j 32 11 replaced replace VBN cord-269720-o81j3d1j 32 12 with with IN cord-269720-o81j3d1j 32 13 medium medium NN cord-269720-o81j3d1j 32 14 containing contain VBG cord-269720-o81j3d1j 32 15 1 1 CD cord-269720-o81j3d1j 32 16 ixg ixg NNP cord-269720-o81j3d1j 32 17 / / SYM cord-269720-o81j3d1j 32 18 ml ml NN cord-269720-o81j3d1j 32 19 actinomycin actinomycin NNS cord-269720-o81j3d1j 32 20 D D NNP cord-269720-o81j3d1j 32 21 to to TO cord-269720-o81j3d1j 32 22 inhibit inhibit VB cord-269720-o81j3d1j 32 23 host host NN cord-269720-o81j3d1j 32 24 - - HYPH cord-269720-o81j3d1j 32 25 cell cell NN cord-269720-o81j3d1j 32 26 RNA RNA NNP cord-269720-o81j3d1j 32 27 synthesis synthesis NN cord-269720-o81j3d1j 32 28 ( ( -LRB- cord-269720-o81j3d1j 32 29 21 21 CD cord-269720-o81j3d1j 32 30 ) ) -RRB- cord-269720-o81j3d1j 32 31 . . . cord-269720-o81j3d1j 33 1 After after IN cord-269720-o81j3d1j 33 2 a a DT cord-269720-o81j3d1j 33 3 further further JJ cord-269720-o81j3d1j 33 4 2-hr 2-hr JJ cord-269720-o81j3d1j 33 5 incubation incubation NN cord-269720-o81j3d1j 33 6 , , , cord-269720-o81j3d1j 33 7 25 25 CD cord-269720-o81j3d1j 33 8 r r CD cord-269720-o81j3d1j 33 9 of of IN cord-269720-o81j3d1j 33 10 [ [ -LRB- cord-269720-o81j3d1j 33 11 5,6 5,6 CD cord-269720-o81j3d1j 33 12 - - HYPH cord-269720-o81j3d1j 33 13 3H]uridine 3h]uridine CD cord-269720-o81j3d1j 34 1 ( ( -LRB- cord-269720-o81j3d1j 34 2 Amersham Amersham NNP cord-269720-o81j3d1j 34 3 International International NNP cord-269720-o81j3d1j 34 4 plc plc NNP cord-269720-o81j3d1j 34 5 , , , cord-269720-o81j3d1j 34 6 TRK.410 TRK.410 NNP cord-269720-o81j3d1j 34 7 , , , cord-269720-o81j3d1j 34 8 35 35 CD cord-269720-o81j3d1j 34 9 - - SYM cord-269720-o81j3d1j 34 10 50 50 CD cord-269720-o81j3d1j 34 11 Ci ci NN cord-269720-o81j3d1j 34 12 / / SYM cord-269720-o81j3d1j 34 13 mM mm NN cord-269720-o81j3d1j 34 14 ) ) -RRB- cord-269720-o81j3d1j 34 15 was be VBD cord-269720-o81j3d1j 34 16 added add VBN cord-269720-o81j3d1j 34 17 per per IN cord-269720-o81j3d1j 34 18 culture culture NN cord-269720-o81j3d1j 34 19 bottle bottle NN cord-269720-o81j3d1j 34 20 and and CC cord-269720-o81j3d1j 34 21 the the DT cord-269720-o81j3d1j 34 22 cells cell NNS cord-269720-o81j3d1j 34 23 were be VBD cord-269720-o81j3d1j 34 24 incubated incubate VBN cord-269720-o81j3d1j 34 25 for for IN cord-269720-o81j3d1j 34 26 a a DT cord-269720-o81j3d1j 34 27 further further JJ cord-269720-o81j3d1j 34 28 5 5 CD cord-269720-o81j3d1j 34 29 hr hr NN cord-269720-o81j3d1j 34 30 . . . cord-269720-o81j3d1j 35 1 The the DT cord-269720-o81j3d1j 35 2 cells cell NNS cord-269720-o81j3d1j 35 3 were be VBD cord-269720-o81j3d1j 35 4 lysed lyse VBN cord-269720-o81j3d1j 35 5 with with IN cord-269720-o81j3d1j 35 6 guanidinium guanidinium NN cord-269720-o81j3d1j 35 7 thiocyanate thiocyanate NNP cord-269720-o81j3d1j 35 8 , , , cord-269720-o81j3d1j 35 9 the the DT cord-269720-o81j3d1j 35 10 RNA RNA NNP cord-269720-o81j3d1j 35 11 pelleted pellete VBD cord-269720-o81j3d1j 35 12 through through IN cord-269720-o81j3d1j 35 13 5.7 5.7 CD cord-269720-o81j3d1j 35 14 M M NNP cord-269720-o81j3d1j 35 15 cesium cesium NN cord-269720-o81j3d1j 35 16 chloride chloride NN cord-269720-o81j3d1j 35 17 and and CC cord-269720-o81j3d1j 35 18 poly(A)-containing poly(a)-containe VBG cord-269720-o81j3d1j 35 19 RNA RNA NNP cord-269720-o81j3d1j 35 20 isolated isolate VBN cord-269720-o81j3d1j 35 21 by by IN cord-269720-o81j3d1j 35 22 poly(U poly(u ADD cord-269720-o81j3d1j 35 23 ) ) -RRB- cord-269720-o81j3d1j 36 1 Sepharose sepharose JJ cord-269720-o81j3d1j 36 2 affinity affinity NN cord-269720-o81j3d1j 36 3 chromatography chromatography NN cord-269720-o81j3d1j 36 4 , , , cord-269720-o81j3d1j 36 5 as as IN cord-269720-o81j3d1j 36 6 described describe VBN cord-269720-o81j3d1j 36 7 previously previously RB cord-269720-o81j3d1j 36 8 ( ( -LRB- cord-269720-o81j3d1j 36 9 21 21 CD cord-269720-o81j3d1j 36 10 ) ) -RRB- cord-269720-o81j3d1j 36 11 . . . cord-269720-o81j3d1j 37 1 Two two CD cord-269720-o81j3d1j 37 2 oligonucleotides oligonucleotide NNS cord-269720-o81j3d1j 37 3 were be VBD cord-269720-o81j3d1j 37 4 synthesized synthesize VBN cord-269720-o81j3d1j 37 5 by by IN cord-269720-o81j3d1j 37 6 the the DT cord-269720-o81j3d1j 37 7 phosphoramidite phosphoramidite JJ cord-269720-o81j3d1j 37 8 method method NN cord-269720-o81j3d1j 37 9 using use VBG cord-269720-o81j3d1j 37 10 an an DT cord-269720-o81j3d1j 37 11 Applied Applied NNP cord-269720-o81j3d1j 37 12 Biosystem Biosystem NNP cord-269720-o81j3d1j 37 13 381A 381A NNP cord-269720-o81j3d1j 37 14 synthesizer synthesizer NN cord-269720-o81j3d1j 37 15 . . . cord-269720-o81j3d1j 38 1 One one CD cord-269720-o81j3d1j 38 2 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 38 3 , , , cord-269720-o81j3d1j 38 4 oligo oligo NNP cord-269720-o81j3d1j 38 5 38 38 CD cord-269720-o81j3d1j 38 6 ( ( -LRB- cord-269720-o81j3d1j 38 7 5'-TGGATT 5'-tggatt CD cord-269720-o81j3d1j 38 8 - - HYPH cord-269720-o81j3d1j 38 9 CATCCCCCCAACTA CATCCCCCCAACTA NNP cord-269720-o81j3d1j 38 10 - - HYPH cord-269720-o81j3d1j 38 11 Y Y NNP cord-269720-o81j3d1j 38 12 ) ) -RRB- cord-269720-o81j3d1j 38 13 , , , cord-269720-o81j3d1j 38 14 was be VBD cord-269720-o81j3d1j 38 15 complementary complementary JJ cord-269720-o81j3d1j 38 16 to to IN cord-269720-o81j3d1j 38 17 the the DT cord-269720-o81j3d1j 38 18 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 38 19 gene gene NNP cord-269720-o81j3d1j 38 20 22 22 CD cord-269720-o81j3d1j 38 21 bp bp NNP cord-269720-o81j3d1j 38 22 downstream downstream RB cord-269720-o81j3d1j 38 23 from from IN cord-269720-o81j3d1j 38 24 the the DT cord-269720-o81j3d1j 38 25 initiation initiation NN cord-269720-o81j3d1j 38 26 ATG ATG NNP cord-269720-o81j3d1j 38 27 codon codon NN cord-269720-o81j3d1j 38 28 ( ( -LRB- cord-269720-o81j3d1j 38 29 15 15 CD cord-269720-o81j3d1j 38 30 ) ) -RRB- cord-269720-o81j3d1j 38 31 , , , cord-269720-o81j3d1j 38 32 as as IN cord-269720-o81j3d1j 38 33 shown show VBN cord-269720-o81j3d1j 38 34 in in IN cord-269720-o81j3d1j 38 35 Fig Fig NNP cord-269720-o81j3d1j 38 36 . . . cord-269720-o81j3d1j 39 1 1 1 CD cord-269720-o81j3d1j 39 2 , , , cord-269720-o81j3d1j 39 3 and and CC cord-269720-o81j3d1j 39 4 was be VBD cord-269720-o81j3d1j 39 5 used use VBN cord-269720-o81j3d1j 39 6 for for IN cord-269720-o81j3d1j 39 7 primer primer NN cord-269720-o81j3d1j 39 8 extension extension NN cord-269720-o81j3d1j 39 9 . . . cord-269720-o81j3d1j 40 1 The the DT cord-269720-o81j3d1j 40 2 second second JJ cord-269720-o81j3d1j 40 3 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 40 4 , , , cord-269720-o81j3d1j 40 5 oligo oligo NNP cord-269720-o81j3d1j 40 6 58 58 CD cord-269720-o81j3d1j 40 7 ( ( -LRB- cord-269720-o81j3d1j 40 8 5'-AGAGATA 5'-agagata CD cord-269720-o81j3d1j 40 9 - - HYPH cord-269720-o81j3d1j 40 10 TAGCCACGCTACACTCACTTTAC TAGCCACGCTACACTCACTTTAC NNP cord-269720-o81j3d1j 40 11 - - HYPH cord-269720-o81j3d1j 40 12 Y Y NNP cord-269720-o81j3d1j 40 13 ) ) -RRB- cord-269720-o81j3d1j 40 14 , , , cord-269720-o81j3d1j 40 15 was be VBD cord-269720-o81j3d1j 40 16 complementary complementary JJ cord-269720-o81j3d1j 40 17 to to IN cord-269720-o81j3d1j 40 18 the the DT cord-269720-o81j3d1j 40 19 5 5 CD cord-269720-o81j3d1j 40 20 ' ' NN cord-269720-o81j3d1j 40 21 end end NN cord-269720-o81j3d1j 40 22 of of IN cord-269720-o81j3d1j 40 23 the the DT cord-269720-o81j3d1j 40 24 leader leader NN cord-269720-o81j3d1j 40 25 RNA RNA NNP cord-269720-o81j3d1j 40 26 ( ( -LRB- cord-269720-o81j3d1j 40 27 Fig Fig NNP cord-269720-o81j3d1j 40 28 . . . cord-269720-o81j3d1j 40 29 1 1 CD cord-269720-o81j3d1j 40 30 ) ) -RRB- cord-269720-o81j3d1j 40 31 and and CC cord-269720-o81j3d1j 40 32 was be VBD cord-269720-o81j3d1j 40 33 used use VBN cord-269720-o81j3d1j 40 34 for for IN cord-269720-o81j3d1j 40 35 Northern northern JJ cord-269720-o81j3d1j 40 36 blot blot NN cord-269720-o81j3d1j 40 37 analysis analysis NN cord-269720-o81j3d1j 40 38 of of IN cord-269720-o81j3d1j 40 39 viral viral JJ cord-269720-o81j3d1j 40 40 mRNA mrna NN cord-269720-o81j3d1j 40 41 . . . cord-269720-o81j3d1j 41 1 Gel gel NN cord-269720-o81j3d1j 41 2 - - HYPH cord-269720-o81j3d1j 41 3 purified purify VBN cord-269720-o81j3d1j 41 4 oligo oligo NN cord-269720-o81j3d1j 41 5 38 38 CD cord-269720-o81j3d1j 41 6 ( ( -LRB- cord-269720-o81j3d1j 41 7 500 500 CD cord-269720-o81j3d1j 41 8 ng ng NN cord-269720-o81j3d1j 41 9 ) ) -RRB- cord-269720-o81j3d1j 41 10 was be VBD cord-269720-o81j3d1j 41 11 5'-end 5'-end CD cord-269720-o81j3d1j 41 12 - - HYPH cord-269720-o81j3d1j 41 13 labeled label VBN cord-269720-o81j3d1j 41 14 ( ( -LRB- cord-269720-o81j3d1j 41 15 22 22 CD cord-269720-o81j3d1j 41 16 ) ) -RRB- cord-269720-o81j3d1j 41 17 using use VBG cord-269720-o81j3d1j 41 18 20 20 CD cord-269720-o81j3d1j 41 19 U U NNP cord-269720-o81j3d1j 41 20 of of IN cord-269720-o81j3d1j 41 21 T T NNP cord-269720-o81j3d1j 41 22 4 4 CD cord-269720-o81j3d1j 41 23 polynucleotide polynucleotide NN cord-269720-o81j3d1j 41 24 kinase kinase NN cord-269720-o81j3d1j 41 25 ( ( -LRB- cord-269720-o81j3d1j 41 26 Gibco Gibco NNP cord-269720-o81j3d1j 41 27 - - HYPH cord-269720-o81j3d1j 41 28 BRL BRL NNP cord-269720-o81j3d1j 41 29 , , , cord-269720-o81j3d1j 41 30 Paisley Paisley NNP cord-269720-o81j3d1j 41 31 ) ) -RRB- cord-269720-o81j3d1j 41 32 and and CC cord-269720-o81j3d1j 41 33 20 20 CD cord-269720-o81j3d1j 41 34 IxCi IxCi NNP cord-269720-o81j3d1j 41 35 [ [ -LRB- cord-269720-o81j3d1j 41 36 ~/-32p]ATP ~/-32p]ATP NFP cord-269720-o81j3d1j 42 1 ( ( -LRB- cord-269720-o81j3d1j 42 2 Amersham Amersham NNP cord-269720-o81j3d1j 42 3 International International NNP cord-269720-o81j3d1j 42 4 plc plc NNP cord-269720-o81j3d1j 42 5 , , , cord-269720-o81j3d1j 42 6 PB PB NNP cord-269720-o81j3d1j 42 7 10168 10168 CD cord-269720-o81j3d1j 42 8 , , , cord-269720-o81j3d1j 42 9 3000 3000 CD cord-269720-o81j3d1j 42 10 ci ci NNP cord-269720-o81j3d1j 42 11 / / SYM cord-269720-o81j3d1j 42 12 mM. mM. NNP cord-269720-o81j3d1j 42 13 Poly(A)-containing poly(a)-containe VBG cord-269720-o81j3d1j 42 14 RNA RNA NNP cord-269720-o81j3d1j 42 15 ( ( -LRB- cord-269720-o81j3d1j 42 16 1.5 1.5 CD cord-269720-o81j3d1j 42 17 p p NN cord-269720-o81j3d1j 42 18 ~ ~ CD cord-269720-o81j3d1j 42 19 g g NN cord-269720-o81j3d1j 42 20 ) ) -RRB- cord-269720-o81j3d1j 42 21 isolated isolate VBN cord-269720-o81j3d1j 42 22 from from IN cord-269720-o81j3d1j 42 23 TGEV TGEV NNP cord-269720-o81j3d1j 42 24 - - HYPH cord-269720-o81j3d1j 42 25 and and CC cord-269720-o81j3d1j 42 26 PRCV PRCV NNP cord-269720-o81j3d1j 42 27 - - HYPH cord-269720-o81j3d1j 42 28 infected infect VBN cord-269720-o81j3d1j 42 29 cells cell NNS cord-269720-o81j3d1j 42 30 was be VBD cord-269720-o81j3d1j 42 31 resuspended resuspend VBN cord-269720-o81j3d1j 42 32 in in IN cord-269720-o81j3d1j 42 33 water water NN cord-269720-o81j3d1j 42 34 and and CC cord-269720-o81j3d1j 42 35 heated heat VBN cord-269720-o81j3d1j 42 36 at at IN cord-269720-o81j3d1j 42 37 60~ 60~ CD cord-269720-o81j3d1j 42 38 for for IN cord-269720-o81j3d1j 42 39 3 3 CD cord-269720-o81j3d1j 42 40 min min NN cord-269720-o81j3d1j 42 41 . . . cord-269720-o81j3d1j 43 1 A a DT cord-269720-o81j3d1j 43 2 further further JJ cord-269720-o81j3d1j 43 3 incubation incubation NN cord-269720-o81j3d1j 43 4 was be VBD cord-269720-o81j3d1j 43 5 carried carry VBN cord-269720-o81j3d1j 43 6 out out RP cord-269720-o81j3d1j 43 7 using use VBG cord-269720-o81j3d1j 43 8 the the DT cord-269720-o81j3d1j 43 9 two two CD cord-269720-o81j3d1j 43 10 mRNA mRNA NNP cord-269720-o81j3d1j 43 11 preparations preparation NNS cord-269720-o81j3d1j 43 12 in in IN cord-269720-o81j3d1j 43 13 27 27 CD cord-269720-o81j3d1j 43 14 p.l p.l JJ cord-269720-o81j3d1j 43 15 reaction reaction NN cord-269720-o81j3d1j 43 16 volumes volume NNS cord-269720-o81j3d1j 43 17 containing contain VBG cord-269720-o81j3d1j 43 18 40 40 CD cord-269720-o81j3d1j 43 19 U U NNP cord-269720-o81j3d1j 43 20 of of IN cord-269720-o81j3d1j 43 21 RNasin RNasin NNP cord-269720-o81j3d1j 44 1 ( ( -LRB- cord-269720-o81j3d1j 44 2 Promega Promega NNP cord-269720-o81j3d1j 44 3 Biotec Biotec NNP cord-269720-o81j3d1j 44 4 , , , cord-269720-o81j3d1j 44 5 Liverpool Liverpool NNP cord-269720-o81j3d1j 44 6 ) ) -RRB- cord-269720-o81j3d1j 44 7 , , , cord-269720-o81j3d1j 44 8 50 50 CD cord-269720-o81j3d1j 44 9 mM mm CD cord-269720-o81j3d1j 44 10 Tris Tris NNP cord-269720-o81j3d1j 44 11 - - HYPH cord-269720-o81j3d1j 44 12 HC1 HC1 NNP cord-269720-o81j3d1j 44 13 ( ( -LRB- cord-269720-o81j3d1j 44 14 pH pH NNP cord-269720-o81j3d1j 44 15 8.3 8.3 CD cord-269720-o81j3d1j 44 16 ) ) -RRB- cord-269720-o81j3d1j 44 17 , , . cord-269720-o81j3d1j 45 1 10 10 CD cord-269720-o81j3d1j 45 2 mM mm CD cord-269720-o81j3d1j 45 3 MgC12 MgC12 NNP cord-269720-o81j3d1j 45 4 , , , cord-269720-o81j3d1j 45 5 35 35 CD cord-269720-o81j3d1j 45 6 mM mm CD cord-269720-o81j3d1j 45 7 KC1 KC1 NNP cord-269720-o81j3d1j 45 8 , , , cord-269720-o81j3d1j 45 9 30 30 CD cord-269720-o81j3d1j 45 10 mM mm CD cord-269720-o81j3d1j 45 11 2-mercaptoethanol 2-mercaptoethanol CD cord-269720-o81j3d1j 45 12 , , , cord-269720-o81j3d1j 45 13 3 3 CD cord-269720-o81j3d1j 45 14 mM mm NN cord-269720-o81j3d1j 45 15 dithiothreitol dithiothreitol NN cord-269720-o81j3d1j 45 16 , , , cord-269720-o81j3d1j 45 17 4 4 CD cord-269720-o81j3d1j 45 18 mM mm CD cord-269720-o81j3d1j 45 19 dNTPs dntp NNS cord-269720-o81j3d1j 45 20 , , , cord-269720-o81j3d1j 45 21 5'-end 5'-end CD cord-269720-o81j3d1j 45 22 - - HYPH cord-269720-o81j3d1j 45 23 labeled label VBN cord-269720-o81j3d1j 45 24 oligo oligo NN cord-269720-o81j3d1j 45 25 38 38 CD cord-269720-o81j3d1j 45 26 ( ( -LRB- cord-269720-o81j3d1j 45 27 120 120 CD cord-269720-o81j3d1j 45 28 ng ng NN cord-269720-o81j3d1j 45 29 ) ) -RRB- cord-269720-o81j3d1j 45 30 , , , cord-269720-o81j3d1j 45 31 and and CC cord-269720-o81j3d1j 45 32 21 21 CD cord-269720-o81j3d1j 45 33 U U NNP cord-269720-o81j3d1j 45 34 of of IN cord-269720-o81j3d1j 45 35 AMV AMV NNP cord-269720-o81j3d1j 45 36 reverse reverse NN cord-269720-o81j3d1j 45 37 transcriptase transcriptase NN cord-269720-o81j3d1j 45 38 ( ( -LRB- cord-269720-o81j3d1j 45 39 Super Super NNP cord-269720-o81j3d1j 45 40 - - NNP cord-269720-o81j3d1j 45 41 RT RT NNP cord-269720-o81j3d1j 45 42 , , , cord-269720-o81j3d1j 45 43 Anglian Anglian NNP cord-269720-o81j3d1j 45 44 Biotech Biotech NNP cord-269720-o81j3d1j 45 45 Ltd Ltd NNP cord-269720-o81j3d1j 45 46 , , , cord-269720-o81j3d1j 45 47 Colchester Colchester NNP cord-269720-o81j3d1j 45 48 ) ) -RRB- cord-269720-o81j3d1j 45 49 for for IN cord-269720-o81j3d1j 45 50 90 90 CD cord-269720-o81j3d1j 45 51 min min NN cord-269720-o81j3d1j 45 52 at at IN cord-269720-o81j3d1j 45 53 42~ 42~ CD cord-269720-o81j3d1j 45 54 Formamide Formamide NNP cord-269720-o81j3d1j 45 55 dye dye NN cord-269720-o81j3d1j 45 56 ( ( -LRB- cord-269720-o81j3d1j 45 57 80 80 CD cord-269720-o81j3d1j 45 58 % % NN cord-269720-o81j3d1j 45 59 formamide formamide NN cord-269720-o81j3d1j 45 60 , , , cord-269720-o81j3d1j 45 61 10 10 CD cord-269720-o81j3d1j 45 62 mM mm CD cord-269720-o81j3d1j 45 63 NaOH NaOH NNP cord-269720-o81j3d1j 45 64 , , , cord-269720-o81j3d1j 45 65 1 1 CD cord-269720-o81j3d1j 45 66 mM mm CD cord-269720-o81j3d1j 45 67 EDTA EDTA NNP cord-269720-o81j3d1j 45 68 , , , cord-269720-o81j3d1j 45 69 0.1 0.1 CD cord-269720-o81j3d1j 45 70 % % NN cord-269720-o81j3d1j 45 71 xylene xylene NN cord-269720-o81j3d1j 45 72 cylanol cylanol NN cord-269720-o81j3d1j 45 73 blue blue NN cord-269720-o81j3d1j 45 74 , , , cord-269720-o81j3d1j 45 75 0.1 0.1 CD cord-269720-o81j3d1j 45 76 % % NN cord-269720-o81j3d1j 45 77 bromophenol bromophenol NN cord-269720-o81j3d1j 45 78 blue blue NNP cord-269720-o81j3d1j 45 79 ) ) -RRB- cord-269720-o81j3d1j 45 80 was be VBD cord-269720-o81j3d1j 45 81 added add VBN cord-269720-o81j3d1j 45 82 and and CC cord-269720-o81j3d1j 45 83 the the DT cord-269720-o81j3d1j 45 84 mixture mixture NN cord-269720-o81j3d1j 45 85 boiled boil VBD cord-269720-o81j3d1j 45 86 for for IN cord-269720-o81j3d1j 45 87 3 3 CD cord-269720-o81j3d1j 45 88 min min NN cord-269720-o81j3d1j 45 89 and and CC cord-269720-o81j3d1j 45 90 electrophoresed electrophorese VBD cord-269720-o81j3d1j 45 91 on on IN cord-269720-o81j3d1j 45 92 a a DT cord-269720-o81j3d1j 45 93 40 40 CD cord-269720-o81j3d1j 45 94 cm cm NN cord-269720-o81j3d1j 45 95 buffer buffer NN cord-269720-o81j3d1j 45 96 gradient gradient NN cord-269720-o81j3d1j 45 97 sequencing sequencing NN cord-269720-o81j3d1j 45 98 gel gel NN cord-269720-o81j3d1j 45 99 ( ( -LRB- cord-269720-o81j3d1j 45 100 23 23 CD cord-269720-o81j3d1j 45 101 ) ) -RRB- cord-269720-o81j3d1j 45 102 . . . cord-269720-o81j3d1j 46 1 The the DT cord-269720-o81j3d1j 46 2 wet wet JJ cord-269720-o81j3d1j 46 3 gel gel NN cord-269720-o81j3d1j 46 4 was be VBD cord-269720-o81j3d1j 46 5 autoradiographed autoradiographe VBN cord-269720-o81j3d1j 46 6 for for IN cord-269720-o81j3d1j 46 7 1 1 CD cord-269720-o81j3d1j 46 8 hr hr NN cord-269720-o81j3d1j 46 9 to to TO cord-269720-o81j3d1j 46 10 locate locate VB cord-269720-o81j3d1j 46 11 the the DT cord-269720-o81j3d1j 46 12 primerextended primerextende VBN cord-269720-o81j3d1j 46 13 products product NNS cord-269720-o81j3d1j 46 14 , , , cord-269720-o81j3d1j 46 15 which which WDT cord-269720-o81j3d1j 46 16 were be VBD cord-269720-o81j3d1j 46 17 excised excise VBN cord-269720-o81j3d1j 46 18 from from IN cord-269720-o81j3d1j 46 19 the the DT cord-269720-o81j3d1j 46 20 gel gel NN cord-269720-o81j3d1j 46 21 . . . cord-269720-o81j3d1j 47 1 The the DT cord-269720-o81j3d1j 47 2 labeled label VBN cord-269720-o81j3d1j 47 3 fragments fragment NNS cord-269720-o81j3d1j 47 4 were be VBD cord-269720-o81j3d1j 47 5 eluted elute VBN cord-269720-o81j3d1j 47 6 from from IN cord-269720-o81j3d1j 47 7 the the DT cord-269720-o81j3d1j 47 8 polyacrylamide polyacrylamide NN cord-269720-o81j3d1j 47 9 gel gel NN cord-269720-o81j3d1j 47 10 and and CC cord-269720-o81j3d1j 47 11 chemically chemically RB cord-269720-o81j3d1j 47 12 cleaved cleave VBN cord-269720-o81j3d1j 47 13 ( ( -LRB- cord-269720-o81j3d1j 47 14 24 24 CD cord-269720-o81j3d1j 47 15 ) ) -RRB- cord-269720-o81j3d1j 47 16 . . . cord-269720-o81j3d1j 48 1 Samples sample NNS cord-269720-o81j3d1j 48 2 of of IN cord-269720-o81j3d1j 48 3 the the DT cord-269720-o81j3d1j 48 4 cleaved cleaved JJ cord-269720-o81j3d1j 48 5 products product NNS cord-269720-o81j3d1j 48 6 from from IN cord-269720-o81j3d1j 48 7 each each DT cord-269720-o81j3d1j 48 8 of of IN cord-269720-o81j3d1j 48 9 the the DT cord-269720-o81j3d1j 48 10 primer primer NN cord-269720-o81j3d1j 48 11 extended extend VBN cord-269720-o81j3d1j 48 12 products product NNS cord-269720-o81j3d1j 48 13 were be VBD cord-269720-o81j3d1j 48 14 electrophoresed electrophorese VBN cord-269720-o81j3d1j 48 15 on on IN cord-269720-o81j3d1j 48 16 6 6 CD cord-269720-o81j3d1j 48 17 % % NN cord-269720-o81j3d1j 48 18 polyacrylamide polyacrylamide NN cord-269720-o81j3d1j 48 19 gels gel NNS cord-269720-o81j3d1j 48 20 at at IN cord-269720-o81j3d1j 48 21 35 35 CD cord-269720-o81j3d1j 48 22 W w NN cord-269720-o81j3d1j 48 23 constant constant JJ cord-269720-o81j3d1j 48 24 power power NN cord-269720-o81j3d1j 48 25 for for IN cord-269720-o81j3d1j 48 26 two two CD cord-269720-o81j3d1j 48 27 different different JJ cord-269720-o81j3d1j 48 28 lengths length NNS cord-269720-o81j3d1j 48 29 of of IN cord-269720-o81j3d1j 48 30 time time NN cord-269720-o81j3d1j 48 31 . . . cord-269720-o81j3d1j 49 1 TGEV TGEV NNP cord-269720-o81j3d1j 49 2 and and CC cord-269720-o81j3d1j 49 3 PRCV PRCV NNP cord-269720-o81j3d1j 50 1 poly(A)-containing poly(A)-containing NNP cord-269720-o81j3d1j 50 2 RNA RNA NNP cord-269720-o81j3d1j 50 3 was be VBD cord-269720-o81j3d1j 50 4 glyoxylated glyoxylate VBN cord-269720-o81j3d1j 50 5 and and CC cord-269720-o81j3d1j 50 6 separated separate VBN cord-269720-o81j3d1j 50 7 on on IN cord-269720-o81j3d1j 50 8 a a DT cord-269720-o81j3d1j 50 9 1 1 CD cord-269720-o81j3d1j 50 10 % % NN cord-269720-o81j3d1j 50 11 agarose agarose NN cord-269720-o81j3d1j 50 12 gel gel NN cord-269720-o81j3d1j 50 13 ( ( -LRB- cord-269720-o81j3d1j 50 14 22 22 CD cord-269720-o81j3d1j 50 15 ) ) -RRB- cord-269720-o81j3d1j 50 16 . . . cord-269720-o81j3d1j 51 1 The the DT cord-269720-o81j3d1j 51 2 RNA RNA NNP cord-269720-o81j3d1j 51 3 was be VBD cord-269720-o81j3d1j 51 4 transferred transfer VBN cord-269720-o81j3d1j 51 5 onto onto IN cord-269720-o81j3d1j 51 6 Biodyne Biodyne NNP cord-269720-o81j3d1j 51 7 A A NNP cord-269720-o81j3d1j 51 8 membranes membrane NNS cord-269720-o81j3d1j 52 1 ( ( -LRB- cord-269720-o81j3d1j 52 2 Pall Pall NNP cord-269720-o81j3d1j 52 3 P P NNP cord-269720-o81j3d1j 52 4 / / SYM cord-269720-o81j3d1j 52 5 N n CD cord-269720-o81j3d1j 53 1 BNNG3R BNNG3R NNP cord-269720-o81j3d1j 53 2 1.2 1.2 CD cord-269720-o81j3d1j 54 1 ~m ~m JJ cord-269720-o81j3d1j 54 2 , , , cord-269720-o81j3d1j 54 3 Gallenkamp Gallenkamp NNP cord-269720-o81j3d1j 54 4 ) ) -RRB- cord-269720-o81j3d1j 54 5 in in IN cord-269720-o81j3d1j 54 6 X20 X20 NNP cord-269720-o81j3d1j 54 7 SSC SSC NNP cord-269720-o81j3d1j 54 8 ( ( -LRB- cord-269720-o81j3d1j 54 9 X1 X1 NNP cord-269720-o81j3d1j 54 10 SSC SSC NNP cord-269720-o81j3d1j 54 11 = = SYM cord-269720-o81j3d1j 54 12 0.15 0.15 CD cord-269720-o81j3d1j 54 13 M M NNP cord-269720-o81j3d1j 54 14 NaCI NaCI NNP cord-269720-o81j3d1j 54 15 , , , cord-269720-o81j3d1j 54 16 0.015 0.015 CD cord-269720-o81j3d1j 54 17 M M NNP cord-269720-o81j3d1j 54 18 trisodium trisodium NN cord-269720-o81j3d1j 54 19 citrate citrate NN cord-269720-o81j3d1j 54 20 , , , cord-269720-o81j3d1j 54 21 pH pH NNP cord-269720-o81j3d1j 54 22 7.0 7.0 CD cord-269720-o81j3d1j 54 23 ) ) -RRB- cord-269720-o81j3d1j 54 24 for for IN cord-269720-o81j3d1j 54 25 18 18 CD cord-269720-o81j3d1j 54 26 hr hr NN cord-269720-o81j3d1j 54 27 and and CC cord-269720-o81j3d1j 54 28 baked bake VBN cord-269720-o81j3d1j 54 29 at at IN cord-269720-o81j3d1j 54 30 80~ 80~ CD cord-269720-o81j3d1j 54 31 for for IN cord-269720-o81j3d1j 54 32 2 2 CD cord-269720-o81j3d1j 54 33 hr hr NN cord-269720-o81j3d1j 54 34 . . . cord-269720-o81j3d1j 55 1 The the DT cord-269720-o81j3d1j 55 2 membrane membrane NN cord-269720-o81j3d1j 55 3 was be VBD cord-269720-o81j3d1j 55 4 boiled boil VBN cord-269720-o81j3d1j 55 5 in in IN cord-269720-o81j3d1j 55 6 50 50 CD cord-269720-o81j3d1j 55 7 mM mm CD cord-269720-o81j3d1j 55 8 Tris Tris NNP cord-269720-o81j3d1j 55 9 - - HYPH cord-269720-o81j3d1j 55 10 HCl HCl NNP cord-269720-o81j3d1j 55 11 pH pH NNP cord-269720-o81j3d1j 55 12 8.0 8.0 CD cord-269720-o81j3d1j 55 13 for for IN cord-269720-o81j3d1j 55 14 5 5 CD cord-269720-o81j3d1j 55 15 min min NN cord-269720-o81j3d1j 55 16 to to TO cord-269720-o81j3d1j 55 17 remove remove VB cord-269720-o81j3d1j 55 18 glyoxal glyoxal NN cord-269720-o81j3d1j 55 19 groups group NNS cord-269720-o81j3d1j 55 20 from from IN cord-269720-o81j3d1j 55 21 the the DT cord-269720-o81j3d1j 55 22 RNA RNA NNP cord-269720-o81j3d1j 55 23 and and CC cord-269720-o81j3d1j 55 24 prehybridized prehybridize VBN cord-269720-o81j3d1j 55 25 in in IN cord-269720-o81j3d1j 55 26 the the DT cord-269720-o81j3d1j 55 27 presence presence NN cord-269720-o81j3d1j 55 28 of of IN cord-269720-o81j3d1j 55 29 50 50 CD cord-269720-o81j3d1j 55 30 % % NN cord-269720-o81j3d1j 55 31 formamide formamide NN cord-269720-o81j3d1j 55 32 for for IN cord-269720-o81j3d1j 55 33 6 6 CD cord-269720-o81j3d1j 55 34 hr hr NN cord-269720-o81j3d1j 55 35 at at IN cord-269720-o81j3d1j 55 36 42~ 42~ CD cord-269720-o81j3d1j 55 37 ( ( -LRB- cord-269720-o81j3d1j 55 38 15 15 CD cord-269720-o81j3d1j 55 39 ) ) -RRB- cord-269720-o81j3d1j 55 40 . . . cord-269720-o81j3d1j 56 1 The the DT cord-269720-o81j3d1j 56 2 viral viral JJ cord-269720-o81j3d1j 56 3 mRNA mrna NN cord-269720-o81j3d1j 56 4 species specie NNS cord-269720-o81j3d1j 56 5 were be VBD cord-269720-o81j3d1j 56 6 hydribidized hydribidize VBN cord-269720-o81j3d1j 56 7 with with IN cord-269720-o81j3d1j 56 8 32p 32p CD cord-269720-o81j3d1j 56 9 - - HYPH cord-269720-o81j3d1j 56 10 labeled label VBN cord-269720-o81j3d1j 56 11 oligo oligo NN cord-269720-o81j3d1j 56 12 58 58 CD cord-269720-o81j3d1j 56 13 in in IN cord-269720-o81j3d1j 56 14 the the DT cord-269720-o81j3d1j 56 15 presence presence NN cord-269720-o81j3d1j 56 16 of of IN cord-269720-o81j3d1j 56 17 50 50 CD cord-269720-o81j3d1j 56 18 % % NN cord-269720-o81j3d1j 56 19 formamide formamide NN cord-269720-o81j3d1j 56 20 for for IN cord-269720-o81j3d1j 56 21 18 18 CD cord-269720-o81j3d1j 56 22 hr hr NN cord-269720-o81j3d1j 56 23 at at IN cord-269720-o81j3d1j 56 24 42~ 42~ CD cord-269720-o81j3d1j 57 1 The the DT cord-269720-o81j3d1j 57 2 membrane membrane NN cord-269720-o81j3d1j 57 3 was be VBD cord-269720-o81j3d1j 57 4 washed wash VBN cord-269720-o81j3d1j 57 5 four four CD cord-269720-o81j3d1j 57 6 times time NNS cord-269720-o81j3d1j 57 7 in in IN cord-269720-o81j3d1j 57 8 X2 X2 NNP cord-269720-o81j3d1j 57 9 SSC SSC NNP cord-269720-o81j3d1j 57 10 containing contain VBG cord-269720-o81j3d1j 57 11 0.1 0.1 CD cord-269720-o81j3d1j 57 12 % % NN cord-269720-o81j3d1j 57 13 NaDodSO nadodso NN cord-269720-o81j3d1j 57 14 4 4 CD cord-269720-o81j3d1j 57 15 for for IN cord-269720-o81j3d1j 57 16 15 15 CD cord-269720-o81j3d1j 57 17 rain rain NN cord-269720-o81j3d1j 57 18 at at IN cord-269720-o81j3d1j 57 19 room room NN cord-269720-o81j3d1j 57 20 temperature temperature NN cord-269720-o81j3d1j 57 21 and and CC cord-269720-o81j3d1j 57 22 autoradiographed autoradiographe VBN cord-269720-o81j3d1j 57 23 . . . cord-269720-o81j3d1j 58 1 Following follow VBG cord-269720-o81j3d1j 58 2 primer primer NN cord-269720-o81j3d1j 58 3 extension extension NN cord-269720-o81j3d1j 58 4 , , , cord-269720-o81j3d1j 58 5 using use VBG cord-269720-o81j3d1j 58 6 oligo oligo NNP cord-269720-o81j3d1j 58 7 38 38 CD cord-269720-o81j3d1j 58 8 at at IN cord-269720-o81j3d1j 58 9 the the DT cord-269720-o81j3d1j 58 10 5 5 CD cord-269720-o81j3d1j 58 11 ' ' NN cord-269720-o81j3d1j 58 12 end end NN cord-269720-o81j3d1j 58 13 of of IN cord-269720-o81j3d1j 58 14 the the DT cord-269720-o81j3d1j 58 15 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 58 16 gene gene NN cord-269720-o81j3d1j 58 17 from from IN cord-269720-o81j3d1j 58 18 the the DT cord-269720-o81j3d1j 58 19 porcine porcine JJ cord-269720-o81j3d1j 58 20 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 58 21 TGEV TGEV NNP cord-269720-o81j3d1j 58 22 and and CC cord-269720-o81j3d1j 58 23 PRCV PRCV NNP cord-269720-o81j3d1j 58 24 , , , cord-269720-o81j3d1j 58 25 labelled label VBN cord-269720-o81j3d1j 58 26 fragments fragment NNS cord-269720-o81j3d1j 58 27 of of IN cord-269720-o81j3d1j 58 28 approximately approximately RB cord-269720-o81j3d1j 58 29 140 140 CD cord-269720-o81j3d1j 58 30 bases basis NNS cord-269720-o81j3d1j 58 31 were be VBD cord-269720-o81j3d1j 58 32 produced produce VBN cord-269720-o81j3d1j 58 33 and and CC cord-269720-o81j3d1j 58 34 purified purify VBN cord-269720-o81j3d1j 58 35 from from IN cord-269720-o81j3d1j 58 36 gels gel NNS cord-269720-o81j3d1j 58 37 . . . cord-269720-o81j3d1j 59 1 Larger large JJR cord-269720-o81j3d1j 59 2 molecular molecular JJ cord-269720-o81j3d1j 59 3 weight weight NN cord-269720-o81j3d1j 59 4 species specie NNS cord-269720-o81j3d1j 59 5 were be VBD cord-269720-o81j3d1j 59 6 also also RB cord-269720-o81j3d1j 59 7 observed observe VBN cord-269720-o81j3d1j 59 8 ( ( -LRB- cord-269720-o81j3d1j 59 9 data datum NNS cord-269720-o81j3d1j 59 10 not not RB cord-269720-o81j3d1j 59 11 shown show VBN cord-269720-o81j3d1j 59 12 ) ) -RRB- cord-269720-o81j3d1j 59 13 in in IN cord-269720-o81j3d1j 59 14 minor minor JJ cord-269720-o81j3d1j 59 15 amounts amount NNS cord-269720-o81j3d1j 59 16 , , , cord-269720-o81j3d1j 59 17 presumably presumably RB cord-269720-o81j3d1j 59 18 corresponding correspond VBG cord-269720-o81j3d1j 59 19 to to TO cord-269720-o81j3d1j 59 20 read read VB cord-269720-o81j3d1j 59 21 - - HYPH cord-269720-o81j3d1j 59 22 through through RP cord-269720-o81j3d1j 59 23 sequences sequence NNS cord-269720-o81j3d1j 59 24 upstream upstream RB cord-269720-o81j3d1j 59 25 of of IN cord-269720-o81j3d1j 59 26 the the DT cord-269720-o81j3d1j 59 27 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 59 28 gene gene NN cord-269720-o81j3d1j 59 29 primed prime VBN cord-269720-o81j3d1j 59 30 from from IN cord-269720-o81j3d1j 59 31 the the DT cord-269720-o81j3d1j 59 32 larger large JJR cord-269720-o81j3d1j 59 33 mRNA mRNA NNP cord-269720-o81j3d1j 59 34 species specie NNS cord-269720-o81j3d1j 59 35 . . . cord-269720-o81j3d1j 60 1 The the DT cord-269720-o81j3d1j 60 2 nucleotide nucleotide JJ cord-269720-o81j3d1j 60 3 sequences sequence NNS cord-269720-o81j3d1j 60 4 of of IN cord-269720-o81j3d1j 60 5 the the DT cord-269720-o81j3d1j 60 6 two two CD cord-269720-o81j3d1j 60 7 fragments fragment NNS cord-269720-o81j3d1j 60 8 , , , cord-269720-o81j3d1j 60 9 determined determine VBN cord-269720-o81j3d1j 60 10 by by IN cord-269720-o81j3d1j 60 11 chemical chemical NN cord-269720-o81j3d1j 60 12 cleavage cleavage NN cord-269720-o81j3d1j 60 13 , , , cord-269720-o81j3d1j 60 14 were be VBD cord-269720-o81j3d1j 60 15 identical identical JJ cord-269720-o81j3d1j 60 16 . . . cord-269720-o81j3d1j 61 1 The the DT cord-269720-o81j3d1j 61 2 resulting result VBG cord-269720-o81j3d1j 61 3 nucleotide nucleotide JJ cord-269720-o81j3d1j 61 4 sequence sequence NN cord-269720-o81j3d1j 61 5 of of IN cord-269720-o81j3d1j 61 6 the the DT cord-269720-o81j3d1j 61 7 TGEV TGEV NNP cord-269720-o81j3d1j 61 8 leader leader NN cord-269720-o81j3d1j 61 9 RNA RNA NNP cord-269720-o81j3d1j 61 10 sequence sequence NN cord-269720-o81j3d1j 61 11 is be VBZ cord-269720-o81j3d1j 61 12 shown show VBN cord-269720-o81j3d1j 61 13 in in IN cord-269720-o81j3d1j 61 14 relation relation NN cord-269720-o81j3d1j 61 15 to to IN cord-269720-o81j3d1j 61 16 the the DT cord-269720-o81j3d1j 61 17 TGEV TGEV NNP cord-269720-o81j3d1j 61 18 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 61 19 gene gene NN cord-269720-o81j3d1j 61 20 in in IN cord-269720-o81j3d1j 61 21 Fig Fig NNP cord-269720-o81j3d1j 61 22 . . . cord-269720-o81j3d1j 62 1 1 1 LS cord-269720-o81j3d1j 62 2 . . . cord-269720-o81j3d1j 63 1 The the DT cord-269720-o81j3d1j 63 2 leader leader NN cord-269720-o81j3d1j 63 3 RNA RNA NNP cord-269720-o81j3d1j 63 4 sequence sequence NN cord-269720-o81j3d1j 63 5 diverges diverge VBZ cord-269720-o81j3d1j 63 6 from from IN cord-269720-o81j3d1j 63 7 the the DT cord-269720-o81j3d1j 63 8 genomic genomic JJ cord-269720-o81j3d1j 63 9 sequence sequence NN cord-269720-o81j3d1j 63 10 15 15 CD cord-269720-o81j3d1j 63 11 bp bp NNP cord-269720-o81j3d1j 63 12 upstream upstream RB cord-269720-o81j3d1j 63 13 of of IN cord-269720-o81j3d1j 63 14 the the DT cord-269720-o81j3d1j 63 15 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 63 16 gene gene NN cord-269720-o81j3d1j 63 17 , , , cord-269720-o81j3d1j 63 18 corresponding correspond VBG cord-269720-o81j3d1j 63 19 to to IN cord-269720-o81j3d1j 63 20 the the DT cord-269720-o81j3d1j 63 21 first first JJ cord-269720-o81j3d1j 63 22 nucleotide nucleotide NN cord-269720-o81j3d1j 63 23 of of IN cord-269720-o81j3d1j 63 24 the the DT cord-269720-o81j3d1j 63 25 membrane membrane NN cord-269720-o81j3d1j 63 26 protein protein NN cord-269720-o81j3d1j 63 27 gene gene NN cord-269720-o81j3d1j 63 28 stop stop NN cord-269720-o81j3d1j 63 29 codon codon NNP cord-269720-o81j3d1j 63 30 ( ( -LRB- cord-269720-o81j3d1j 63 31 16 16 CD cord-269720-o81j3d1j 63 32 ) ) -RRB- cord-269720-o81j3d1j 63 33 , , , cord-269720-o81j3d1j 63 34 indicating indicate VBG cord-269720-o81j3d1j 63 35 a a DT cord-269720-o81j3d1j 63 36 length length NN cord-269720-o81j3d1j 63 37 of of IN cord-269720-o81j3d1j 63 38 91 91 CD cord-269720-o81j3d1j 63 39 nucleotides nucleotide NNS cord-269720-o81j3d1j 63 40 of of IN cord-269720-o81j3d1j 63 41 unique unique JJ cord-269720-o81j3d1j 63 42 sequence sequence NN cord-269720-o81j3d1j 64 1 ( ( -LRB- cord-269720-o81j3d1j 64 2 Fig Fig NNP cord-269720-o81j3d1j 64 3 . . NNP cord-269720-o81j3d1j 64 4 1 1 CD cord-269720-o81j3d1j 64 5 ) ) -RRB- cord-269720-o81j3d1j 64 6 . . . cord-269720-o81j3d1j 65 1 The the DT cord-269720-o81j3d1j 65 2 91 91 CD cord-269720-o81j3d1j 65 3 nucleotide nucleotide JJ cord-269720-o81j3d1j 65 4 leader leader NN cord-269720-o81j3d1j 65 5 sequence sequence NN cord-269720-o81j3d1j 65 6 of of IN cord-269720-o81j3d1j 65 7 TGEV TGEV NNP cord-269720-o81j3d1j 65 8 and and CC cord-269720-o81j3d1j 65 9 PRCV PRCV NNP cord-269720-o81j3d1j 65 10 has have VBZ cord-269720-o81j3d1j 65 11 a a DT cord-269720-o81j3d1j 65 12 low low JJ cord-269720-o81j3d1j 65 13 content content NN cord-269720-o81j3d1j 65 14 of of IN cord-269720-o81j3d1j 65 15 G g NN cord-269720-o81j3d1j 65 16 ( ( -LRB- cord-269720-o81j3d1j 65 17 18 18 CD cord-269720-o81j3d1j 65 18 % % NN cord-269720-o81j3d1j 65 19 ) ) -RRB- cord-269720-o81j3d1j 65 20 and and CC cord-269720-o81j3d1j 65 21 C C NNP cord-269720-o81j3d1j 65 22 ( ( -LRB- cord-269720-o81j3d1j 65 23 20 20 CD cord-269720-o81j3d1j 65 24 % % NN cord-269720-o81j3d1j 65 25 ) ) -RRB- cord-269720-o81j3d1j 65 26 , , , cord-269720-o81j3d1j 65 27 and and CC cord-269720-o81j3d1j 65 28 a a DT cord-269720-o81j3d1j 65 29 high high JJ cord-269720-o81j3d1j 65 30 A a NN cord-269720-o81j3d1j 65 31 ( ( -LRB- cord-269720-o81j3d1j 65 32 22 22 CD cord-269720-o81j3d1j 65 33 % % NN cord-269720-o81j3d1j 65 34 ) ) -RRB- cord-269720-o81j3d1j 65 35 and and CC cord-269720-o81j3d1j 65 36 T T NNP cord-269720-o81j3d1j 65 37 ( ( -LRB- cord-269720-o81j3d1j 65 38 40 40 CD cord-269720-o81j3d1j 65 39 % % NN cord-269720-o81j3d1j 65 40 ) ) -RRB- cord-269720-o81j3d1j 65 41 content content NN cord-269720-o81j3d1j 65 42 , , , cord-269720-o81j3d1j 65 43 with with IN cord-269720-o81j3d1j 65 44 20 20 CD cord-269720-o81j3d1j 65 45 % % NN cord-269720-o81j3d1j 65 46 of of IN cord-269720-o81j3d1j 65 47 the the DT cord-269720-o81j3d1j 65 48 T T NNP cord-269720-o81j3d1j 65 49 residues residue NNS cord-269720-o81j3d1j 65 50 grouped group VBN cord-269720-o81j3d1j 65 51 in in IN cord-269720-o81j3d1j 65 52 threeto threeto NNP cord-269720-o81j3d1j 65 53 four four CD cord-269720-o81j3d1j 65 54 - - HYPH cord-269720-o81j3d1j 65 55 nucleotide nucleotide JJ cord-269720-o81j3d1j 65 56 motifs motif NNS cord-269720-o81j3d1j 65 57 ( ( -LRB- cord-269720-o81j3d1j 65 58 Fig fig NN cord-269720-o81j3d1j 65 59 . . . cord-269720-o81j3d1j 65 60 1 1 CD cord-269720-o81j3d1j 65 61 ) ) -RRB- cord-269720-o81j3d1j 65 62 . . . cord-269720-o81j3d1j 66 1 These these DT cord-269720-o81j3d1j 66 2 values value NNS cord-269720-o81j3d1j 66 3 are be VBP cord-269720-o81j3d1j 66 4 similar similar JJ cord-269720-o81j3d1j 66 5 to to IN cord-269720-o81j3d1j 66 6 those those DT cord-269720-o81j3d1j 66 7 observed observe VBN cord-269720-o81j3d1j 66 8 from from IN cord-269720-o81j3d1j 66 9 the the DT cord-269720-o81j3d1j 66 10 TGEV TGEV NNP cord-269720-o81j3d1j 66 11 genome genome NN cord-269720-o81j3d1j 66 12 so so RB cord-269720-o81j3d1j 66 13 far far RB cord-269720-o81j3d1j 66 14 sequenced sequence VBN cord-269720-o81j3d1j 66 15 , , , cord-269720-o81j3d1j 66 16 except except IN cord-269720-o81j3d1j 66 17 that that IN cord-269720-o81j3d1j 66 18 the the DT cord-269720-o81j3d1j 66 19 values value NNS cord-269720-o81j3d1j 66 20 for for IN cord-269720-o81j3d1j 66 21 A a NN cord-269720-o81j3d1j 66 22 ( ( -LRB- cord-269720-o81j3d1j 66 23 30.5 30.5 CD cord-269720-o81j3d1j 66 24 % % NN cord-269720-o81j3d1j 66 25 ) ) -RRB- cord-269720-o81j3d1j 66 26 and and CC cord-269720-o81j3d1j 66 27 T T NNP cord-269720-o81j3d1j 66 28 ( ( -LRB- cord-269720-o81j3d1j 66 29 32.1 32.1 CD cord-269720-o81j3d1j 66 30 % % NN cord-269720-o81j3d1j 66 31 ) ) -RRB- cord-269720-o81j3d1j 66 32 are be VBP cord-269720-o81j3d1j 66 33 more more RBR cord-269720-o81j3d1j 66 34 similar similar JJ cord-269720-o81j3d1j 66 35 on on IN cord-269720-o81j3d1j 66 36 the the DT cord-269720-o81j3d1j 66 37 genome genome NN cord-269720-o81j3d1j 66 38 than than IN cord-269720-o81j3d1j 66 39 on on IN cord-269720-o81j3d1j 66 40 the the DT cord-269720-o81j3d1j 66 41 leader leader NN cord-269720-o81j3d1j 66 42 sequence sequence NN cord-269720-o81j3d1j 66 43 . . . cord-269720-o81j3d1j 67 1 Analysis analysis NN cord-269720-o81j3d1j 67 2 of of IN cord-269720-o81j3d1j 67 3 the the DT cord-269720-o81j3d1j 67 4 TGEV TGEV NNP cord-269720-o81j3d1j 67 5 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 67 6 nucleotide nucleotide JJ cord-269720-o81j3d1j 67 7 sequence sequence NN cord-269720-o81j3d1j 67 8 ( ( -LRB- cord-269720-o81j3d1j 67 9 15 15 CD cord-269720-o81j3d1j 67 10 ) ) -RRB- cord-269720-o81j3d1j 67 11 revealed reveal VBD cord-269720-o81j3d1j 67 12 a a DT cord-269720-o81j3d1j 67 13 potential potential JJ cord-269720-o81j3d1j 67 14 RNA rna NN cord-269720-o81j3d1j 67 15 polymerase polymerase NN cord-269720-o81j3d1j 67 16 - - HYPH cord-269720-o81j3d1j 67 17 leader leader NN cord-269720-o81j3d1j 67 18 complex complex JJ cord-269720-o81j3d1j 67 19 binding binding NN cord-269720-o81j3d1j 67 20 site site NN cord-269720-o81j3d1j 67 21 . . . cord-269720-o81j3d1j 68 1 The the DT cord-269720-o81j3d1j 68 2 site site NN cord-269720-o81j3d1j 68 3 , , , cord-269720-o81j3d1j 68 4 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 68 5 , , , cord-269720-o81j3d1j 68 6 is be VBZ cord-269720-o81j3d1j 68 7 seven seven CD cord-269720-o81j3d1j 68 8 nucleotides nucleotide NNS cord-269720-o81j3d1j 68 9 upstream upstream RB cord-269720-o81j3d1j 68 10 of of IN cord-269720-o81j3d1j 68 11 the the DT cord-269720-o81j3d1j 68 12 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 68 13 initiation initiation NNP cord-269720-o81j3d1j 68 14 codon codon NNP cord-269720-o81j3d1j 68 15 and and CC cord-269720-o81j3d1j 68 16 has have VBZ cord-269720-o81j3d1j 68 17 also also RB cord-269720-o81j3d1j 68 18 been be VBN cord-269720-o81j3d1j 68 19 found find VBN cord-269720-o81j3d1j 68 20 to to TO cord-269720-o81j3d1j 68 21 precede precede VB cord-269720-o81j3d1j 68 22 all all PDT cord-269720-o81j3d1j 68 23 the the DT cord-269720-o81j3d1j 68 24 TGEV TGEV NNP cord-269720-o81j3d1j 68 25 structural structural JJ cord-269720-o81j3d1j 68 26 protein protein NN cord-269720-o81j3d1j 68 27 genes gene NNS cord-269720-o81j3d1j 68 28 and and CC cord-269720-o81j3d1j 68 29 two two CD cord-269720-o81j3d1j 68 30 of of IN cord-269720-o81j3d1j 68 31 the the DT cord-269720-o81j3d1j 68 32 three three CD cord-269720-o81j3d1j 68 33 potential potential JJ cord-269720-o81j3d1j 68 34 genes gene NNS cord-269720-o81j3d1j 68 35 shown show VBN cord-269720-o81j3d1j 68 36 to to TO cord-269720-o81j3d1j 68 37 be be VB cord-269720-o81j3d1j 68 38 at at IN cord-269720-o81j3d1j 68 39 the the DT cord-269720-o81j3d1j 68 40 5 5 CD cord-269720-o81j3d1j 68 41 ' ' NN cord-269720-o81j3d1j 68 42 end end NN cord-269720-o81j3d1j 68 43 of of IN cord-269720-o81j3d1j 68 44 mRNA mrna NN cord-269720-o81j3d1j 68 45 species specie NNS cord-269720-o81j3d1j 68 46 ( ( -LRB- cord-269720-o81j3d1j 68 47 15 15 CD cord-269720-o81j3d1j 68 48 ) ) -RRB- cord-269720-o81j3d1j 68 49 ( ( -LRB- cord-269720-o81j3d1j 68 50 16 16 CD cord-269720-o81j3d1j 68 51 ) ) -RRB- cord-269720-o81j3d1j 68 52 ( ( -LRB- cord-269720-o81j3d1j 68 53 17 17 CD cord-269720-o81j3d1j 68 54 ) ) -RRB- cord-269720-o81j3d1j 68 55 . . . cord-269720-o81j3d1j 69 1 This this DT cord-269720-o81j3d1j 69 2 consensus consensus NN cord-269720-o81j3d1j 69 3 sequence sequence NN cord-269720-o81j3d1j 69 4 is be VBZ cord-269720-o81j3d1j 69 5 found find VBN cord-269720-o81j3d1j 69 6 two two CD cord-269720-o81j3d1j 69 7 nucleotides nucleotide NNS cord-269720-o81j3d1j 69 8 downstream downstream JJ cord-269720-o81j3d1j 69 9 of of IN cord-269720-o81j3d1j 69 10 the the DT cord-269720-o81j3d1j 69 11 nucleotide nucleotide NN cord-269720-o81j3d1j 69 12 where where WRB cord-269720-o81j3d1j 69 13 the the DT cord-269720-o81j3d1j 69 14 leader leader NN cord-269720-o81j3d1j 69 15 RNA RNA NNP cord-269720-o81j3d1j 69 16 and and CC cord-269720-o81j3d1j 69 17 TGEV TGEV NNP cord-269720-o81j3d1j 69 18 genomic genomic JJ cord-269720-o81j3d1j 69 19 sequences sequence NNS cord-269720-o81j3d1j 69 20 diverge diverge VBP cord-269720-o81j3d1j 69 21 , , , cord-269720-o81j3d1j 69 22 indicating indicate VBG cord-269720-o81j3d1j 69 23 that that IN cord-269720-o81j3d1j 69 24 this this DT cord-269720-o81j3d1j 69 25 sequence sequence NN cord-269720-o81j3d1j 69 26 is be VBZ cord-269720-o81j3d1j 69 27 involved involve VBN cord-269720-o81j3d1j 69 28 in in IN cord-269720-o81j3d1j 69 29 the the DT cord-269720-o81j3d1j 69 30 leader leader NN cord-269720-o81j3d1j 69 31 - - HYPH cord-269720-o81j3d1j 69 32 primed prime VBN cord-269720-o81j3d1j 69 33 transcription transcription NN cord-269720-o81j3d1j 69 34 ofTGEV oftgev NN cord-269720-o81j3d1j 69 35 mRNA mrna NN cord-269720-o81j3d1j 69 36 molecules molecule NNS cord-269720-o81j3d1j 69 37 . . . cord-269720-o81j3d1j 70 1 As as IN cord-269720-o81j3d1j 70 2 can can MD cord-269720-o81j3d1j 70 3 be be VB cord-269720-o81j3d1j 70 4 seen see VBN cord-269720-o81j3d1j 70 5 from from IN cord-269720-o81j3d1j 70 6 Fig Fig NNP cord-269720-o81j3d1j 70 7 . . . cord-269720-o81j3d1j 71 1 2 2 CD cord-269720-o81j3d1j 71 2 , , , cord-269720-o81j3d1j 71 3 4 4 CD cord-269720-o81j3d1j 71 4 of of IN cord-269720-o81j3d1j 71 5 the the DT cord-269720-o81j3d1j 71 6 6 6 CD cord-269720-o81j3d1j 71 7 mRNA mrna NN cord-269720-o81j3d1j 71 8 species specie NNS cord-269720-o81j3d1j 71 9 from from IN cord-269720-o81j3d1j 71 10 the the DT cord-269720-o81j3d1j 71 11 FS772/70 FS772/70 NNP cord-269720-o81j3d1j 71 12 strain strain NN cord-269720-o81j3d1j 71 13 of of IN cord-269720-o81j3d1j 71 14 TGEV TGEV NNP cord-269720-o81j3d1j 71 15 have have VBP cord-269720-o81j3d1j 71 16 the the DT cord-269720-o81j3d1j 71 17 sequence sequence NN cord-269720-o81j3d1j 71 18 AACTAAAC aactaaac NN cord-269720-o81j3d1j 71 19 , , , cord-269720-o81j3d1j 71 20 of of IN cord-269720-o81j3d1j 71 21 which which WDT cord-269720-o81j3d1j 71 22 the the DT cord-269720-o81j3d1j 71 23 5'-end 5'-end CD cord-269720-o81j3d1j 71 24 adenosine adenosine NN cord-269720-o81j3d1j 71 25 residue residue NN cord-269720-o81j3d1j 71 26 is be VBZ cord-269720-o81j3d1j 71 27 the the DT cord-269720-o81j3d1j 71 28 next next JJ cord-269720-o81j3d1j 71 29 base base NN cord-269720-o81j3d1j 71 30 down down RP cord-269720-o81j3d1j 71 31 from from IN cord-269720-o81j3d1j 71 32 the the DT cord-269720-o81j3d1j 71 33 divergence divergence NN cord-269720-o81j3d1j 71 34 point point NN cord-269720-o81j3d1j 71 35 . . . cord-269720-o81j3d1j 72 1 In in IN cord-269720-o81j3d1j 72 2 fact fact NN cord-269720-o81j3d1j 72 3 , , , cord-269720-o81j3d1j 72 4 the the DT cord-269720-o81j3d1j 72 5 consensus consensus NN cord-269720-o81j3d1j 72 6 sequence sequence NN cord-269720-o81j3d1j 72 7 at at IN cord-269720-o81j3d1j 72 8 the the DT cord-269720-o81j3d1j 72 9 spike spike NN cord-269720-o81j3d1j 72 10 / / SYM cord-269720-o81j3d1j 72 11 ORF1-ORF2 ORF1-ORF2 NNP cord-269720-o81j3d1j 72 12 gene gene NN cord-269720-o81j3d1j 72 13 junction junction NN cord-269720-o81j3d1j 72 14 has have VBZ cord-269720-o81j3d1j 72 15 the the DT cord-269720-o81j3d1j 72 16 sequence sequence NN cord-269720-o81j3d1j 72 17 GAACTAAAC GAACTAAAC NNP cord-269720-o81j3d1j 72 18 and and CC cord-269720-o81j3d1j 72 19 at at IN cord-269720-o81j3d1j 72 20 the the DT cord-269720-o81j3d1j 72 21 NUC NUC NNP cord-269720-o81j3d1j 72 22 / / SYM cord-269720-o81j3d1j 72 23 ORF4 ORF4 NNP cord-269720-o81j3d1j 72 24 gene gene NN cord-269720-o81j3d1j 72 25 junction junction NN cord-269720-o81j3d1j 72 26 has have VBZ cord-269720-o81j3d1j 72 27 the the DT cord-269720-o81j3d1j 72 28 sequence sequence NN cord-269720-o81j3d1j 72 29 CGAACTAAAC CGAACTAAAC NNP cord-269720-o81j3d1j 72 30 , , , cord-269720-o81j3d1j 72 31 indicating indicate VBG cord-269720-o81j3d1j 72 32 that that IN cord-269720-o81j3d1j 72 33 the the DT cord-269720-o81j3d1j 72 34 region region NN cord-269720-o81j3d1j 72 35 of of IN cord-269720-o81j3d1j 72 36 the the DT cord-269720-o81j3d1j 72 37 leader leader NN cord-269720-o81j3d1j 72 38 sequence sequence NN cord-269720-o81j3d1j 72 39 5 5 CD cord-269720-o81j3d1j 72 40 ' ' '' cord-269720-o81j3d1j 72 41 to to IN cord-269720-o81j3d1j 72 42 the the DT cord-269720-o81j3d1j 72 43 homology homology NN cord-269720-o81j3d1j 72 44 motif motif NN cord-269720-o81j3d1j 72 45 , , , cord-269720-o81j3d1j 72 46 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 72 47 , , , cord-269720-o81j3d1j 72 48 may may MD cord-269720-o81j3d1j 72 49 vary vary VB cord-269720-o81j3d1j 72 50 between between IN cord-269720-o81j3d1j 72 51 89 89 CD cord-269720-o81j3d1j 72 52 and and CC cord-269720-o81j3d1j 72 53 91 91 CD cord-269720-o81j3d1j 72 54 nucleotides nucleotide NNS cord-269720-o81j3d1j 72 55 depending depend VBG cord-269720-o81j3d1j 72 56 on on IN cord-269720-o81j3d1j 72 57 the the DT cord-269720-o81j3d1j 72 58 TGEV TGEV NNP cord-269720-o81j3d1j 72 59 gene gene NN cord-269720-o81j3d1j 72 60 . . . cord-269720-o81j3d1j 73 1 Computer computer NN cord-269720-o81j3d1j 73 2 analysis analysis NN cord-269720-o81j3d1j 73 3 has have VBZ cord-269720-o81j3d1j 73 4 also also RB cord-269720-o81j3d1j 73 5 detected detect VBN cord-269720-o81j3d1j 73 6 a a DT cord-269720-o81j3d1j 73 7 homology homology NN cord-269720-o81j3d1j 73 8 between between IN cord-269720-o81j3d1j 73 9 the the DT cord-269720-o81j3d1j 73 10 leader leader NN cord-269720-o81j3d1j 73 11 RNA RNA NNP cord-269720-o81j3d1j 73 12 sequence sequence NN cord-269720-o81j3d1j 73 13 and and CC cord-269720-o81j3d1j 73 14 the the DT cord-269720-o81j3d1j 73 15 5 5 CD cord-269720-o81j3d1j 73 16 ' ' NN cord-269720-o81j3d1j 73 17 end end NN cord-269720-o81j3d1j 73 18 of of IN cord-269720-o81j3d1j 73 19 the the DT cord-269720-o81j3d1j 73 20 negative negative JJ cord-269720-o81j3d1j 73 21 strand strand NN cord-269720-o81j3d1j 73 22 ( ( -LRB- cord-269720-o81j3d1j 73 23 i.e. i.e. FW cord-269720-o81j3d1j 73 24 , , , cord-269720-o81j3d1j 73 25 the the DT cord-269720-o81j3d1j 73 26 reverse reverse JJ cord-269720-o81j3d1j 73 27 complement complement NN cord-269720-o81j3d1j 73 28 of of IN cord-269720-o81j3d1j 73 29 the the DT cord-269720-o81j3d1j 73 30 noncoding noncoding JJ cord-269720-o81j3d1j 73 31 region region NN cord-269720-o81j3d1j 73 32 at at IN cord-269720-o81j3d1j 73 33 the the DT cord-269720-o81j3d1j 73 34 3 3 CD cord-269720-o81j3d1j 73 35 ' ' NN cord-269720-o81j3d1j 73 36 end end NN cord-269720-o81j3d1j 73 37 of of IN cord-269720-o81j3d1j 73 38 the the DT cord-269720-o81j3d1j 73 39 positive positive JJ cord-269720-o81j3d1j 73 40 strand strand NN cord-269720-o81j3d1j 73 41 ) ) -RRB- cord-269720-o81j3d1j 73 42 . . . cord-269720-o81j3d1j 74 1 This this DT cord-269720-o81j3d1j 74 2 is be VBZ cord-269720-o81j3d1j 74 3 shown show VBN cord-269720-o81j3d1j 74 4 in in IN cord-269720-o81j3d1j 74 5 Fig Fig NNP cord-269720-o81j3d1j 74 6 . . . cord-269720-o81j3d1j 74 7 _SP cord-269720-o81j3d1j 75 1 3 3 LS cord-269720-o81j3d1j 75 2 . . . cord-269720-o81j3d1j 76 1 The the DT cord-269720-o81j3d1j 76 2 nucleotides nucleotide NNS cord-269720-o81j3d1j 76 3 on on IN cord-269720-o81j3d1j 76 4 the the DT cord-269720-o81j3d1j 76 5 leader leader NN cord-269720-o81j3d1j 76 6 RNA RNA NNP cord-269720-o81j3d1j 76 7 sequence sequence NN cord-269720-o81j3d1j 76 8 , , , cord-269720-o81j3d1j 76 9 bases basis NNS cord-269720-o81j3d1j 76 10 84 84 CD cord-269720-o81j3d1j 76 11 - - SYM cord-269720-o81j3d1j 76 12 99 99 CD cord-269720-o81j3d1j 76 13 , , , cord-269720-o81j3d1j 76 14 and and CC cord-269720-o81j3d1j 76 15 on on IN cord-269720-o81j3d1j 76 16 the the DT cord-269720-o81j3d1j 76 17 negative negative JJ cord-269720-o81j3d1j 76 18 strand strand NN cord-269720-o81j3d1j 76 19 , , , cord-269720-o81j3d1j 76 20 bases basis NNS cord-269720-o81j3d1j 76 21 136 136 CD cord-269720-o81j3d1j 76 22 to to TO cord-269720-o81j3d1j 76 23 152 152 CD cord-269720-o81j3d1j 76 24 counting counting NN cord-269720-o81j3d1j 76 25 from from IN cord-269720-o81j3d1j 76 26 the the DT cord-269720-o81j3d1j 76 27 first first JJ cord-269720-o81j3d1j 76 28 base base NN cord-269720-o81j3d1j 76 29 after after IN cord-269720-o81j3d1j 76 30 the the DT cord-269720-o81j3d1j 76 31 poly(A poly(a JJ cord-269720-o81j3d1j 76 32 ) ) -RRB- cord-269720-o81j3d1j 76 33 tail tail NN cord-269720-o81j3d1j 76 34 , , , cord-269720-o81j3d1j 76 35 have have VBP cord-269720-o81j3d1j 76 36 an an DT cord-269720-o81j3d1j 76 37 overall overall JJ cord-269720-o81j3d1j 76 38 homology homology NN cord-269720-o81j3d1j 76 39 of of IN cord-269720-o81j3d1j 76 40 82 82 CD cord-269720-o81j3d1j 76 41 % % NN cord-269720-o81j3d1j 76 42 and and CC cord-269720-o81j3d1j 76 43 include include VBP cord-269720-o81j3d1j 76 44 the the DT cord-269720-o81j3d1j 76 45 sequenc~ sequenc~ NNP cord-269720-o81j3d1j 76 46 CTAAAC CTAAAC NNP cord-269720-o81j3d1j 76 47 , , , cord-269720-o81j3d1j 76 48 which which WDT cord-269720-o81j3d1j 76 49 is be VBZ cord-269720-o81j3d1j 76 50 part part NN cord-269720-o81j3d1j 76 51 of of IN cord-269720-o81j3d1j 76 52 the the DT cord-269720-o81j3d1j 76 53 postulated postulate VBN cord-269720-o81j3d1j 76 54 TGEV TGEV NNP cord-269720-o81j3d1j 76 55 RNA RNA NNP cord-269720-o81j3d1j 76 56 polymerase polymerase NN cord-269720-o81j3d1j 76 57 - - HYPH cord-269720-o81j3d1j 76 58 leader leader NN cord-269720-o81j3d1j 76 59 complex complex JJ cord-269720-o81j3d1j 76 60 binding binding NN cord-269720-o81j3d1j 76 61 site site NN cord-269720-o81j3d1j 76 62 . . . cord-269720-o81j3d1j 77 1 This this DT cord-269720-o81j3d1j 77 2 is be VBZ cord-269720-o81j3d1j 77 3 very very RB cord-269720-o81j3d1j 77 4 similar similar JJ cord-269720-o81j3d1j 77 5 to to IN cord-269720-o81j3d1j 77 6 the the DT cord-269720-o81j3d1j 77 7 observation observation NN cord-269720-o81j3d1j 77 8 for for IN cord-269720-o81j3d1j 77 9 IBV IBV NNP cord-269720-o81j3d1j 77 10 ( ( -LRB- cord-269720-o81j3d1j 77 11 25 25 CD cord-269720-o81j3d1j 77 12 ) ) -RRB- cord-269720-o81j3d1j 77 13 involving involve VBG cord-269720-o81j3d1j 77 14 sequences sequence NNS cord-269720-o81j3d1j 77 15 present present JJ cord-269720-o81j3d1j 77 16 at at IN cord-269720-o81j3d1j 77 17 the the DT cord-269720-o81j3d1j 77 18 5 5 CD cord-269720-o81j3d1j 77 19 ' ' NN cord-269720-o81j3d1j 77 20 end end NN cord-269720-o81j3d1j 77 21 of of IN cord-269720-o81j3d1j 77 22 the the DT cord-269720-o81j3d1j 77 23 IBV IBV NNP cord-269720-o81j3d1j 77 24 genome genome NN cord-269720-o81j3d1j 77 25 , , , cord-269720-o81j3d1j 77 26 and and CC cord-269720-o81j3d1j 77 27 on on IN cord-269720-o81j3d1j 77 28 the the DT cord-269720-o81j3d1j 77 29 IBV IBV NNP cord-269720-o81j3d1j 77 30 leader leader NN cord-269720-o81j3d1j 77 31 RNA RNA NNP cord-269720-o81j3d1j 77 32 sequences sequence NNS cord-269720-o81j3d1j 77 33 , , , cord-269720-o81j3d1j 77 34 with with IN cord-269720-o81j3d1j 77 35 the the DT cord-269720-o81j3d1j 77 36 5 5 CD cord-269720-o81j3d1j 77 37 ' ' NN cord-269720-o81j3d1j 77 38 end end NN cord-269720-o81j3d1j 77 39 of of IN cord-269720-o81j3d1j 77 40 the the DT cord-269720-o81j3d1j 77 41 IBV IBV NNP cord-269720-o81j3d1j 77 42 negative negative JJ cord-269720-o81j3d1j 77 43 strand strand NN cord-269720-o81j3d1j 77 44 . . . cord-269720-o81j3d1j 78 1 The the DT cord-269720-o81j3d1j 78 2 homology homology NN cord-269720-o81j3d1j 78 3 observed observe VBD cord-269720-o81j3d1j 78 4 included include VBD cord-269720-o81j3d1j 78 5 the the DT cord-269720-o81j3d1j 78 6 sequence sequence NN cord-269720-o81j3d1j 78 7 CTTAAC CTTAAC NNP cord-269720-o81j3d1j 78 8 , , , cord-269720-o81j3d1j 78 9 which which WDT cord-269720-o81j3d1j 78 10 is be VBZ cord-269720-o81j3d1j 78 11 part part NN cord-269720-o81j3d1j 78 12 of of IN cord-269720-o81j3d1j 78 13 the the DT cord-269720-o81j3d1j 78 14 postulated postulate VBN cord-269720-o81j3d1j 78 15 IBV IBV NNP cord-269720-o81j3d1j 78 16 RNA RNA NNP cord-269720-o81j3d1j 78 17 polymerase polymerase NN cord-269720-o81j3d1j 78 18 - - HYPH cord-269720-o81j3d1j 78 19 leader leader NN cord-269720-o81j3d1j 78 20 complex complex JJ cord-269720-o81j3d1j 78 21 binding binding NN cord-269720-o81j3d1j 78 22 site site NN cord-269720-o81j3d1j 78 23 CT(T CT(T NNP cord-269720-o81j3d1j 78 24 / / SYM cord-269720-o81j3d1j 78 25 G)AACAA G)AACAA NNP cord-269720-o81j3d1j 78 26 . . . cord-269720-o81j3d1j 79 1 An an DT cord-269720-o81j3d1j 79 2 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 79 3 , , , cord-269720-o81j3d1j 79 4 oligo oligo NNP cord-269720-o81j3d1j 79 5 58 58 CD cord-269720-o81j3d1j 79 6 , , , cord-269720-o81j3d1j 79 7 was be VBD cord-269720-o81j3d1j 79 8 synthesised synthesise VBN cord-269720-o81j3d1j 79 9 that that WDT cord-269720-o81j3d1j 79 10 was be VBD cord-269720-o81j3d1j 79 11 complementary complementary JJ cord-269720-o81j3d1j 79 12 to to IN cord-269720-o81j3d1j 79 13 the the DT cord-269720-o81j3d1j 79 14 5 5 CD cord-269720-o81j3d1j 79 15 ' ' NN cord-269720-o81j3d1j 79 16 end end NN cord-269720-o81j3d1j 79 17 of of IN cord-269720-o81j3d1j 79 18 the the DT cord-269720-o81j3d1j 79 19 TGEV TGEV NNP cord-269720-o81j3d1j 79 20 and and CC cord-269720-o81j3d1j 79 21 PRCV PRCV NNP cord-269720-o81j3d1j 79 22 leader leader NN cord-269720-o81j3d1j 79 23 RNA RNA NNP cord-269720-o81j3d1j 79 24 sequences sequence NNS cord-269720-o81j3d1j 79 25 ( ( -LRB- cord-269720-o81j3d1j 79 26 Fig Fig NNP cord-269720-o81j3d1j 79 27 . . NNP cord-269720-o81j3d1j 79 28 1 1 CD cord-269720-o81j3d1j 79 29 ) ) -RRB- cord-269720-o81j3d1j 79 30 . . . cord-269720-o81j3d1j 80 1 The the DT cord-269720-o81j3d1j 80 2 oligonucleotide oligonucleotide NN cord-269720-o81j3d1j 80 3 was be VBD cord-269720-o81j3d1j 80 4 end end NN cord-269720-o81j3d1j 80 5 - - HYPH cord-269720-o81j3d1j 80 6 labeled label VBN cord-269720-o81j3d1j 80 7 and and CC cord-269720-o81j3d1j 80 8 used use VBN cord-269720-o81j3d1j 80 9 to to TO cord-269720-o81j3d1j 80 10 probe probe VB cord-269720-o81j3d1j 80 11 TGEV TGEV NNP cord-269720-o81j3d1j 80 12 and and CC cord-269720-o81j3d1j 80 13 PRCV PRCV NNP cord-269720-o81j3d1j 80 14 mRNA mRNA NNP cord-269720-o81j3d1j 80 15 species specie NNS cord-269720-o81j3d1j 80 16 that that WDT cord-269720-o81j3d1j 80 17 were be VBD cord-269720-o81j3d1j 80 18 Northern Northern NNP cord-269720-o81j3d1j 80 19 blotted blot VBN cord-269720-o81j3d1j 80 20 onto onto IN cord-269720-o81j3d1j 80 21 Biodyne Biodyne NNP cord-269720-o81j3d1j 80 22 membranes membrane NNS cord-269720-o81j3d1j 80 23 . . . cord-269720-o81j3d1j 81 1 As as IN cord-269720-o81j3d1j 81 2 can can MD cord-269720-o81j3d1j 81 3 be be VB cord-269720-o81j3d1j 81 4 seen see VBN cord-269720-o81j3d1j 81 5 from from IN cord-269720-o81j3d1j 81 6 Fig Fig NNP cord-269720-o81j3d1j 81 7 . . . cord-269720-o81j3d1j 82 1 4 4 LS cord-269720-o81j3d1j 82 2 , , , cord-269720-o81j3d1j 82 3 the the DT cord-269720-o81j3d1j 82 4 labeled label VBN cord-269720-o81j3d1j 82 5 probe probe NN cord-269720-o81j3d1j 82 6 hybridized hybridize VBN cord-269720-o81j3d1j 82 7 to to IN cord-269720-o81j3d1j 82 8 all all DT cord-269720-o81j3d1j 82 9 of of IN cord-269720-o81j3d1j 82 10 the the DT cord-269720-o81j3d1j 82 11 TGEV TGEV NNP cord-269720-o81j3d1j 82 12 and and CC cord-269720-o81j3d1j 82 13 PRCV PRCV NNP cord-269720-o81j3d1j 82 14 mRNA mRNA NNP cord-269720-o81j3d1j 82 15 species specie NNS cord-269720-o81j3d1j 82 16 . . . cord-269720-o81j3d1j 83 1 The the DT cord-269720-o81j3d1j 83 2 intensity intensity NN cord-269720-o81j3d1j 83 3 of of IN cord-269720-o81j3d1j 83 4 the the DT cord-269720-o81j3d1j 83 5 bands band NNS cord-269720-o81j3d1j 83 6 corresponding correspond VBG cord-269720-o81j3d1j 83 7 to to IN cord-269720-o81j3d1j 83 8 labeled label VBN cord-269720-o81j3d1j 83 9 probe probe NN cord-269720-o81j3d1j 83 10 hybridized hybridize VBD cord-269720-o81j3d1j 83 11 the the DT cord-269720-o81j3d1j 83 12 spike spike NN cord-269720-o81j3d1j 83 13 mRNA mrna NN cord-269720-o81j3d1j 83 14 species specie NNS cord-269720-o81j3d1j 83 15 , , , cord-269720-o81j3d1j 83 16 and and CC cord-269720-o81j3d1j 83 17 genomic genomic JJ cord-269720-o81j3d1j 83 18 RNA RNA NNP cord-269720-o81j3d1j 83 19 was be VBD cord-269720-o81j3d1j 83 20 lower low JJR cord-269720-o81j3d1j 83 21 than than IN cord-269720-o81j3d1j 83 22 that that DT cord-269720-o81j3d1j 83 23 observed observe VBD cord-269720-o81j3d1j 83 24 for for IN cord-269720-o81j3d1j 83 25 the the DT cord-269720-o81j3d1j 83 26 smaller small JJR cord-269720-o81j3d1j 83 27 mRNA mRNA NNP cord-269720-o81j3d1j 83 28 species specie NNS cord-269720-o81j3d1j 83 29 due due JJ cord-269720-o81j3d1j 83 30 to to IN cord-269720-o81j3d1j 83 31 less less JJR cord-269720-o81j3d1j 83 32 of of IN cord-269720-o81j3d1j 83 33 these these DT cord-269720-o81j3d1j 83 34 larger large JJR cord-269720-o81j3d1j 83 35 species specie NNS cord-269720-o81j3d1j 83 36 being be VBG cord-269720-o81j3d1j 83 37 isolated isolate VBN cord-269720-o81j3d1j 83 38 from from IN cord-269720-o81j3d1j 83 39 the the DT cord-269720-o81j3d1j 83 40 poly(U poly(u NN cord-269720-o81j3d1j 83 41 ) ) -RRB- cord-269720-o81j3d1j 84 1 Sepharose Sepharose NNP cord-269720-o81j3d1j 84 2 column column NN cord-269720-o81j3d1j 84 3 used use VBN cord-269720-o81j3d1j 84 4 in in IN cord-269720-o81j3d1j 84 5 the the DT cord-269720-o81j3d1j 84 6 isolation isolation NN cord-269720-o81j3d1j 84 7 of of IN cord-269720-o81j3d1j 84 8 mRNA mRNA NNP cord-269720-o81j3d1j 84 9 . . . cord-269720-o81j3d1j 85 1 The the DT cord-269720-o81j3d1j 85 2 fact fact NN cord-269720-o81j3d1j 85 3 that that IN cord-269720-o81j3d1j 85 4 the the DT cord-269720-o81j3d1j 85 5 probe probe NN cord-269720-o81j3d1j 85 6 hybridized hybridize VBD cord-269720-o81j3d1j 85 7 to to IN cord-269720-o81j3d1j 85 8 all all DT cord-269720-o81j3d1j 85 9 of of IN cord-269720-o81j3d1j 85 10 the the DT cord-269720-o81j3d1j 85 11 mRNA mRNA NNP cord-269720-o81j3d1j 85 12 species specie NNS cord-269720-o81j3d1j 85 13 showed show VBD cord-269720-o81j3d1j 85 14 that that IN cord-269720-o81j3d1j 85 15 the the DT cord-269720-o81j3d1j 85 16 leader leader NN cord-269720-o81j3d1j 85 17 RNA RNA NNP cord-269720-o81j3d1j 85 18 sequence sequence NN cord-269720-o81j3d1j 85 19 was be VBD cord-269720-o81j3d1j 85 20 present present JJ cord-269720-o81j3d1j 85 21 on on IN cord-269720-o81j3d1j 85 22 the the DT cord-269720-o81j3d1j 85 23 other other JJ cord-269720-o81j3d1j 85 24 RNA RNA NNP cord-269720-o81j3d1j 85 25 molecules molecule NNS cord-269720-o81j3d1j 85 26 of of IN cord-269720-o81j3d1j 85 27 TGEV TGEV NNS cord-269720-o81j3d1j 85 28 and and CC cord-269720-o81j3d1j 85 29 both both DT cord-269720-o81j3d1j 85 30 strains strain NNS cord-269720-o81j3d1j 85 31 of of IN cord-269720-o81j3d1j 85 32 PRCV PRCV NNP cord-269720-o81j3d1j 85 33 was be VBD cord-269720-o81j3d1j 85 34 not not RB cord-269720-o81j3d1j 85 35 unique unique JJ cord-269720-o81j3d1j 85 36 to to IN cord-269720-o81j3d1j 85 37 the the DT cord-269720-o81j3d1j 85 38 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 85 39 mRNA mRNA NNP cord-269720-o81j3d1j 85 40 species species NNP cord-269720-o81j3d1j 85 41 . . . cord-269720-o81j3d1j 86 1 The the DT cord-269720-o81j3d1j 86 2 two two CD cord-269720-o81j3d1j 86 3 porcine porcine JJ cord-269720-o81j3d1j 86 4 coronavirus coronavirus NN cord-269720-o81j3d1j 86 5 leader leader NN cord-269720-o81j3d1j 86 6 sequences sequence NNS cord-269720-o81j3d1j 86 7 were be VBD cord-269720-o81j3d1j 86 8 identical identical JJ cord-269720-o81j3d1j 86 9 , , , cord-269720-o81j3d1j 86 10 indicating indicate VBG cord-269720-o81j3d1j 86 11 that that IN cord-269720-o81j3d1j 86 12 the the DT cord-269720-o81j3d1j 86 13 two two CD cord-269720-o81j3d1j 86 14 viruses virus NNS cord-269720-o81j3d1j 86 15 probably probably RB cord-269720-o81j3d1j 86 16 use use VBP cord-269720-o81j3d1j 86 17 the the DT cord-269720-o81j3d1j 86 18 same same JJ cord-269720-o81j3d1j 86 19 RNA RNA NNP cord-269720-o81j3d1j 86 20 polymerase polymerase NN cord-269720-o81j3d1j 86 21 - - HYPH cord-269720-o81j3d1j 86 22 leader leader NN cord-269720-o81j3d1j 86 23 complex complex JJ cord-269720-o81j3d1j 86 24 binding binding NN cord-269720-o81j3d1j 86 25 site site NN cord-269720-o81j3d1j 86 26 , , , cord-269720-o81j3d1j 86 27 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 86 28 , , , cord-269720-o81j3d1j 86 29 for for IN cord-269720-o81j3d1j 86 30 the the DT cord-269720-o81j3d1j 86 31 synthesis synthesis NN cord-269720-o81j3d1j 86 32 of of IN cord-269720-o81j3d1j 86 33 subgenomic subgenomic NNP cord-269720-o81j3d1j 86 34 mRNA mRNA NNP cord-269720-o81j3d1j 86 35 species species NNP cord-269720-o81j3d1j 86 36 . . . cord-269720-o81j3d1j 87 1 The the DT cord-269720-o81j3d1j 87 2 SEQHP SEQHP NNP cord-269720-o81j3d1j 87 3 comparison comparison NN cord-269720-o81j3d1j 87 4 program program NN cord-269720-o81j3d1j 87 5 of of IN cord-269720-o81j3d1j 87 6 the the DT cord-269720-o81j3d1j 87 7 Los Los NNP cord-269720-o81j3d1j 87 8 Alamos Alamos NNP cord-269720-o81j3d1j 87 9 ( ( -LRB- cord-269720-o81j3d1j 87 10 26 26 CD cord-269720-o81j3d1j 87 11 ) ) -RRB- cord-269720-o81j3d1j 87 12 package package NN cord-269720-o81j3d1j 87 13 was be VBD cord-269720-o81j3d1j 87 14 used use VBN cord-269720-o81j3d1j 87 15 to to TO cord-269720-o81j3d1j 87 16 compare compare VB cord-269720-o81j3d1j 87 17 the the DT cord-269720-o81j3d1j 87 18 leader leader NN cord-269720-o81j3d1j 87 19 RNA RNA NNP cord-269720-o81j3d1j 87 20 sequences sequence NNS cord-269720-o81j3d1j 87 21 determined determine VBN cord-269720-o81j3d1j 87 22 in in IN cord-269720-o81j3d1j 87 23 this this DT cord-269720-o81j3d1j 87 24 paper paper NN cord-269720-o81j3d1j 87 25 and and CC cord-269720-o81j3d1j 87 26 those those DT cord-269720-o81j3d1j 87 27 published publish VBN cord-269720-o81j3d1j 87 28 for for IN cord-269720-o81j3d1j 87 29 five five CD cord-269720-o81j3d1j 87 30 other other JJ cord-269720-o81j3d1j 87 31 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 87 32 belonging belong VBG cord-269720-o81j3d1j 87 33 to to IN cord-269720-o81j3d1j 87 34 two two CD cord-269720-o81j3d1j 87 35 different different JJ cord-269720-o81j3d1j 87 36 serogroups serogroup NNS cord-269720-o81j3d1j 87 37 . . . cord-269720-o81j3d1j 88 1 The the DT cord-269720-o81j3d1j 88 2 sequences sequence NNS cord-269720-o81j3d1j 88 3 were be VBD cord-269720-o81j3d1j 88 4 compared compare VBN cord-269720-o81j3d1j 88 5 from from IN cord-269720-o81j3d1j 88 6 the the DT cord-269720-o81j3d1j 88 7 5 5 CD cord-269720-o81j3d1j 88 8 ' ' '' cord-269720-o81j3d1j 88 9 ends end NNS cord-269720-o81j3d1j 88 10 to to IN cord-269720-o81j3d1j 88 11 the the DT cord-269720-o81j3d1j 88 12 point point NN cord-269720-o81j3d1j 88 13 of of IN cord-269720-o81j3d1j 88 14 divergence divergence NN cord-269720-o81j3d1j 88 15 from from IN cord-269720-o81j3d1j 88 16 the the DT cord-269720-o81j3d1j 88 17 genomic genomic JJ cord-269720-o81j3d1j 88 18 sequences sequence NNS cord-269720-o81j3d1j 88 19 . . . cord-269720-o81j3d1j 89 1 The the DT cord-269720-o81j3d1j 89 2 percentage percentage NN cord-269720-o81j3d1j 89 3 homologies homology NNS cord-269720-o81j3d1j 89 4 , , , cord-269720-o81j3d1j 89 5 Table table NN cord-269720-o81j3d1j 89 6 1 1 CD cord-269720-o81j3d1j 89 7 , , , cord-269720-o81j3d1j 89 8 were be VBD cord-269720-o81j3d1j 89 9 expressed express VBN cord-269720-o81j3d1j 89 10 as as IN cord-269720-o81j3d1j 89 11 the the DT cord-269720-o81j3d1j 89 12 number number NN cord-269720-o81j3d1j 89 13 of of IN cord-269720-o81j3d1j 89 14 bases basis NNS cord-269720-o81j3d1j 89 15 matched match VBN cord-269720-o81j3d1j 89 16 to to IN cord-269720-o81j3d1j 89 17 the the DT cord-269720-o81j3d1j 89 18 longer long JJR cord-269720-o81j3d1j 89 19 of of IN cord-269720-o81j3d1j 89 20 the the DT cord-269720-o81j3d1j 89 21 two two CD cord-269720-o81j3d1j 89 22 sequences sequence NNS cord-269720-o81j3d1j 89 23 being be VBG cord-269720-o81j3d1j 89 24 compared compare VBN cord-269720-o81j3d1j 89 25 . . . cord-269720-o81j3d1j 90 1 The the DT cord-269720-o81j3d1j 90 2 homology homology NN cord-269720-o81j3d1j 90 3 of of IN cord-269720-o81j3d1j 90 4 the the DT cord-269720-o81j3d1j 90 5 leader leader NN cord-269720-o81j3d1j 90 6 sequences sequence NNS cord-269720-o81j3d1j 90 7 fell fall VBD cord-269720-o81j3d1j 90 8 into into IN cord-269720-o81j3d1j 90 9 three three CD cord-269720-o81j3d1j 90 10 groups group NNS cord-269720-o81j3d1j 90 11 . . . cord-269720-o81j3d1j 91 1 Leader leader NN cord-269720-o81j3d1j 91 2 RNAs rna NNS cord-269720-o81j3d1j 91 3 from from IN cord-269720-o81j3d1j 91 4 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 91 5 belonging belong VBG cord-269720-o81j3d1j 91 6 to to IN cord-269720-o81j3d1j 91 7 different different JJ cord-269720-o81j3d1j 91 8 serological serological JJ cord-269720-o81j3d1j 91 9 groups group NNS cord-269720-o81j3d1j 91 10 had have VBD cord-269720-o81j3d1j 91 11 homologies homology NNS cord-269720-o81j3d1j 91 12 in in IN cord-269720-o81j3d1j 91 13 the the DT cord-269720-o81j3d1j 91 14 region region NN cord-269720-o81j3d1j 91 15 of of IN cord-269720-o81j3d1j 91 16 35 35 CD cord-269720-o81j3d1j 91 17 - - SYM cord-269720-o81j3d1j 91 18 40 40 CD cord-269720-o81j3d1j 91 19 % % NN cord-269720-o81j3d1j 91 20 . . . cord-269720-o81j3d1j 92 1 Serologically serologically RB cord-269720-o81j3d1j 92 2 related related JJ cord-269720-o81j3d1j 92 3 viruses virus NNS cord-269720-o81j3d1j 92 4 like like IN cord-269720-o81j3d1j 92 5 human human JJ cord-269720-o81j3d1j 92 6 coronavirus coronavirus NN cord-269720-o81j3d1j 92 7 ( ( -LRB- cord-269720-o81j3d1j 92 8 HCV HCV NNP cord-269720-o81j3d1j 92 9 ) ) -RRB- cord-269720-o81j3d1j 92 10 ( ( -LRB- cord-269720-o81j3d1j 92 11 strain strain VBP cord-269720-o81j3d1j 92 12 OC43 OC43 NNP cord-269720-o81j3d1j 92 13 ) ) -RRB- cord-269720-o81j3d1j 92 14 and and CC cord-269720-o81j3d1j 92 15 MHV MHV NNP cord-269720-o81j3d1j 92 16 ( ( -LRB- cord-269720-o81j3d1j 92 17 strains strain NNS cord-269720-o81j3d1j 92 18 A59 A59 NNP cord-269720-o81j3d1j 92 19 and and CC cord-269720-o81j3d1j 92 20 JHM JHM NNP cord-269720-o81j3d1j 92 21 ) ) -RRB- cord-269720-o81j3d1j 92 22 have have VBP cord-269720-o81j3d1j 92 23 about about RB cord-269720-o81j3d1j 92 24 60 60 CD cord-269720-o81j3d1j 92 25 % % NN cord-269720-o81j3d1j 92 26 homology homology NN cord-269720-o81j3d1j 92 27 . . . cord-269720-o81j3d1j 93 1 The the DT cord-269720-o81j3d1j 93 2 third third JJ cord-269720-o81j3d1j 93 3 group group NN cord-269720-o81j3d1j 93 4 involved involve VBD cord-269720-o81j3d1j 93 5 different different JJ cord-269720-o81j3d1j 93 6 strains strain NNS cord-269720-o81j3d1j 93 7 of of IN cord-269720-o81j3d1j 93 8 MHV MHV NNP cord-269720-o81j3d1j 93 9 , , , cord-269720-o81j3d1j 93 10 A59 A59 NNP cord-269720-o81j3d1j 93 11 , , , cord-269720-o81j3d1j 93 12 and and CC cord-269720-o81j3d1j 93 13 JHM JHM NNP cord-269720-o81j3d1j 93 14 , , , cord-269720-o81j3d1j 93 15 which which WDT cord-269720-o81j3d1j 93 16 showed show VBD cord-269720-o81j3d1j 93 17 a a DT cord-269720-o81j3d1j 93 18 homology homology NN cord-269720-o81j3d1j 93 19 of of IN cord-269720-o81j3d1j 93 20 91 91 CD cord-269720-o81j3d1j 93 21 % % NN cord-269720-o81j3d1j 93 22 . . . cord-269720-o81j3d1j 94 1 This this DT cord-269720-o81j3d1j 94 2 observation observation NN cord-269720-o81j3d1j 94 3 indicates indicate VBZ cord-269720-o81j3d1j 94 4 that that IN cord-269720-o81j3d1j 94 5 TGEV TGEV NNP cord-269720-o81j3d1j 94 6 and and CC cord-269720-o81j3d1j 94 7 PRCV PRCV NNP cord-269720-o81j3d1j 94 8 , , , cord-269720-o81j3d1j 94 9 which which WDT cord-269720-o81j3d1j 94 10 have have VBP cord-269720-o81j3d1j 94 11 a a DT cord-269720-o81j3d1j 94 12 homology homology NN cord-269720-o81j3d1j 94 13 of of IN cord-269720-o81j3d1j 94 14 100 100 CD cord-269720-o81j3d1j 94 15 % % NN cord-269720-o81j3d1j 94 16 , , , cord-269720-o81j3d1j 94 17 are be VBP cord-269720-o81j3d1j 94 18 probably probably RB cord-269720-o81j3d1j 94 19 different different JJ cord-269720-o81j3d1j 94 20 strains strain NNS cord-269720-o81j3d1j 94 21 of of IN cord-269720-o81j3d1j 94 22 the the DT cord-269720-o81j3d1j 94 23 same same JJ cord-269720-o81j3d1j 94 24 virus virus NN cord-269720-o81j3d1j 94 25 or or CC cord-269720-o81j3d1j 94 26 that that IN cord-269720-o81j3d1j 94 27 PRCV PRCV NNP cord-269720-o81j3d1j 94 28 has have VBZ cord-269720-o81j3d1j 94 29 very very RB cord-269720-o81j3d1j 94 30 recently recently RB cord-269720-o81j3d1j 94 31 diverged diverge VBN cord-269720-o81j3d1j 94 32 from from IN cord-269720-o81j3d1j 94 33 TGEV TGEV NNP cord-269720-o81j3d1j 94 34 . . . cord-269720-o81j3d1j 95 1 In in IN cord-269720-o81j3d1j 95 2 order order NN cord-269720-o81j3d1j 95 3 to to TO cord-269720-o81j3d1j 95 4 identify identify VB cord-269720-o81j3d1j 95 5 common common JJ cord-269720-o81j3d1j 95 6 areas area NNS cord-269720-o81j3d1j 95 7 of of IN cord-269720-o81j3d1j 95 8 homology homology NN cord-269720-o81j3d1j 95 9 , , , cord-269720-o81j3d1j 95 10 the the DT cord-269720-o81j3d1j 95 11 leader leader NN cord-269720-o81j3d1j 95 12 RNA RNA NNP cord-269720-o81j3d1j 95 13 sequences sequence NNS cord-269720-o81j3d1j 95 14 from from IN cord-269720-o81j3d1j 95 15 seven seven CD cord-269720-o81j3d1j 95 16 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 95 17 were be VBD cord-269720-o81j3d1j 95 18 aligned align VBN cord-269720-o81j3d1j 95 19 . . . cord-269720-o81j3d1j 96 1 As as IN cord-269720-o81j3d1j 96 2 can can MD cord-269720-o81j3d1j 96 3 be be VB cord-269720-o81j3d1j 96 4 seen see VBN cord-269720-o81j3d1j 96 5 from from IN cord-269720-o81j3d1j 96 6 Fig Fig NNP cord-269720-o81j3d1j 96 7 . . . cord-269720-o81j3d1j 97 1 5 5 LS cord-269720-o81j3d1j 97 2 , , , cord-269720-o81j3d1j 97 3 these these DT cord-269720-o81j3d1j 97 4 fell fall VBD cord-269720-o81j3d1j 97 5 into into IN cord-269720-o81j3d1j 97 6 two two CD cord-269720-o81j3d1j 97 7 groups group NNS cord-269720-o81j3d1j 97 8 . . . cord-269720-o81j3d1j 98 1 One one CD cord-269720-o81j3d1j 98 2 group group NN cord-269720-o81j3d1j 98 3 consists consist VBZ cord-269720-o81j3d1j 98 4 of of IN cord-269720-o81j3d1j 98 5 MHV MHV NNP cord-269720-o81j3d1j 98 6 ( ( -LRB- cord-269720-o81j3d1j 98 7 strains strain NNS cord-269720-o81j3d1j 98 8 A59 A59 NNP cord-269720-o81j3d1j 98 9 and and CC cord-269720-o81j3d1j 98 10 JHM JHM NNP cord-269720-o81j3d1j 98 11 ) ) -RRB- cord-269720-o81j3d1j 98 12 with with IN cord-269720-o81j3d1j 98 13 HCV HCV NNP cord-269720-o81j3d1j 98 14 ( ( -LRB- cord-269720-o81j3d1j 98 15 OC43 OC43 NNP cord-269720-o81j3d1j 98 16 ) ) -RRB- cord-269720-o81j3d1j 98 17 , , , cord-269720-o81j3d1j 98 18 which which WDT cord-269720-o81j3d1j 98 19 have have VBP cord-269720-o81j3d1j 98 20 a a DT cord-269720-o81j3d1j 98 21 fairly fairly RB cord-269720-o81j3d1j 98 22 high high JJ cord-269720-o81j3d1j 98 23 degree degree NN cord-269720-o81j3d1j 98 24 of of IN cord-269720-o81j3d1j 98 25 homology homology NN cord-269720-o81j3d1j 98 26 along along IN cord-269720-o81j3d1j 98 27 their -PRON- PRP$ cord-269720-o81j3d1j 98 28 lengths length NNS cord-269720-o81j3d1j 98 29 . . . cord-269720-o81j3d1j 99 1 The the DT cord-269720-o81j3d1j 99 2 other other JJ cord-269720-o81j3d1j 99 3 group group NN cord-269720-o81j3d1j 99 4 consists consist VBZ cord-269720-o81j3d1j 99 5 of of IN cord-269720-o81j3d1j 99 6 TGEV TGEV NNP cord-269720-o81j3d1j 99 7 and and CC cord-269720-o81j3d1j 99 8 PRCV PRCV NNP cord-269720-o81j3d1j 99 9 ( ( -LRB- cord-269720-o81j3d1j 99 10 not not RB cord-269720-o81j3d1j 99 11 shown show VBN cord-269720-o81j3d1j 99 12 on on IN cord-269720-o81j3d1j 99 13 the the DT cord-269720-o81j3d1j 99 14 diagram diagram NN cord-269720-o81j3d1j 99 15 ) ) -RRB- cord-269720-o81j3d1j 99 16 with with IN cord-269720-o81j3d1j 99 17 HCV HCV NNP cord-269720-o81j3d1j 99 18 ( ( -LRB- cord-269720-o81j3d1j 99 19 229E 229e CD cord-269720-o81j3d1j 99 20 ) ) -RRB- cord-269720-o81j3d1j 99 21 and and CC cord-269720-o81j3d1j 99 22 IBV IBV NNP cord-269720-o81j3d1j 99 23 , , , cord-269720-o81j3d1j 99 24 which which WDT cord-269720-o81j3d1j 99 25 have have VBP cord-269720-o81j3d1j 99 26 high high JJ cord-269720-o81j3d1j 99 27 homologies homology NNS cord-269720-o81j3d1j 99 28 at at IN cord-269720-o81j3d1j 99 29 their -PRON- PRP$ cord-269720-o81j3d1j 99 30 3 3 CD cord-269720-o81j3d1j 99 31 ' ' '' cord-269720-o81j3d1j 99 32 ends end NNS cord-269720-o81j3d1j 99 33 and and CC cord-269720-o81j3d1j 99 34 areas area NNS cord-269720-o81j3d1j 99 35 of of IN cord-269720-o81j3d1j 99 36 homology homology NN cord-269720-o81j3d1j 99 37 at at IN cord-269720-o81j3d1j 99 38 their -PRON- PRP$ cord-269720-o81j3d1j 99 39 5 5 CD cord-269720-o81j3d1j 99 40 ' ' '' cord-269720-o81j3d1j 99 41 ends end NNS cord-269720-o81j3d1j 99 42 . . . cord-269720-o81j3d1j 100 1 There there EX cord-269720-o81j3d1j 100 2 are be VBP cord-269720-o81j3d1j 100 3 good good JJ cord-269720-o81j3d1j 100 4 homologies homology NNS cord-269720-o81j3d1j 100 5 towards towards IN cord-269720-o81j3d1j 100 6 the the DT cord-269720-o81j3d1j 100 7 3 3 CD cord-269720-o81j3d1j 100 8 ' ' '' cord-269720-o81j3d1j 100 9 ends end NNS cord-269720-o81j3d1j 100 10 , , , cord-269720-o81j3d1j 100 11 involving involve VBG cord-269720-o81j3d1j 100 12 the the DT cord-269720-o81j3d1j 100 13 postulated postulate VBN cord-269720-o81j3d1j 100 14 RNA RNA NNP cord-269720-o81j3d1j 100 15 polymerase polymerase NN cord-269720-o81j3d1j 100 16 - - HYPH cord-269720-o81j3d1j 100 17 leader leader NN cord-269720-o81j3d1j 100 18 complex complex JJ cord-269720-o81j3d1j 100 19 binding binding NN cord-269720-o81j3d1j 100 20 sites site NNS cord-269720-o81j3d1j 100 21 and and CC cord-269720-o81j3d1j 100 22 sequences sequence NNS cord-269720-o81j3d1j 100 23 upstream upstream RB cord-269720-o81j3d1j 100 24 of of IN cord-269720-o81j3d1j 100 25 these these DT cord-269720-o81j3d1j 100 26 sites site NNS cord-269720-o81j3d1j 100 27 , , , cord-269720-o81j3d1j 100 28 between between IN cord-269720-o81j3d1j 100 29 the the DT cord-269720-o81j3d1j 100 30 groups group NNS cord-269720-o81j3d1j 100 31 , , , cord-269720-o81j3d1j 100 32 but but CC cord-269720-o81j3d1j 100 33 very very RB cord-269720-o81j3d1j 100 34 little little JJ cord-269720-o81j3d1j 100 35 if if IN cord-269720-o81j3d1j 100 36 any any DT cord-269720-o81j3d1j 100 37 homology homology NN cord-269720-o81j3d1j 100 38 between between IN cord-269720-o81j3d1j 100 39 the the DT cord-269720-o81j3d1j 100 40 5 5 CD cord-269720-o81j3d1j 100 41 ' ' '' cord-269720-o81j3d1j 100 42 ends end NNS cord-269720-o81j3d1j 100 43 . . . cord-269720-o81j3d1j 101 1 ( ( -LRB- cord-269720-o81j3d1j 101 2 7 7 CD cord-269720-o81j3d1j 101 3 ) ) -RRB- cord-269720-o81j3d1j 101 4 and and CC cord-269720-o81j3d1j 101 5 strain strain VB cord-269720-o81j3d1j 101 6 JHM JHM NNP cord-269720-o81j3d1j 101 7 ( ( -LRB- cord-269720-o81j3d1j 101 8 13 13 CD cord-269720-o81j3d1j 101 9 ) ) -RRB- cord-269720-o81j3d1j 102 1 ; ; : cord-269720-o81j3d1j 102 2 avian avian JJ cord-269720-o81j3d1j 102 3 , , , cord-269720-o81j3d1j 102 4 IBV IBV NNP cord-269720-o81j3d1j 102 5 strain strain VBP cord-269720-o81j3d1j 102 6 Beaudette Beaudette NNP cord-269720-o81j3d1j 102 7 ( ( -LRB- cord-269720-o81j3d1j 102 8 9,25 9,25 CD cord-269720-o81j3d1j 102 9 ) ) -RRB- cord-269720-o81j3d1j 102 10 . . . cord-269720-o81j3d1j 103 1 As as IN cord-269720-o81j3d1j 103 2 seen see VBN cord-269720-o81j3d1j 103 3 from from IN cord-269720-o81j3d1j 103 4 Fig Fig NNP cord-269720-o81j3d1j 103 5 . . . cord-269720-o81j3d1j 104 1 5 5 LS cord-269720-o81j3d1j 105 1 simple simple JJ cord-269720-o81j3d1j 105 2 alignment alignment NN cord-269720-o81j3d1j 105 3 did do VBD cord-269720-o81j3d1j 105 4 not not RB cord-269720-o81j3d1j 105 5 reveal reveal VB cord-269720-o81j3d1j 105 6 very very RB cord-269720-o81j3d1j 105 7 much much JJ cord-269720-o81j3d1j 105 8 information information NN cord-269720-o81j3d1j 105 9 about about IN cord-269720-o81j3d1j 105 10 the the DT cord-269720-o81j3d1j 105 11 homologies homology NNS cord-269720-o81j3d1j 105 12 of of IN cord-269720-o81j3d1j 105 13 the the DT cord-269720-o81j3d1j 105 14 leader leader NN cord-269720-o81j3d1j 105 15 RNA RNA NNP cord-269720-o81j3d1j 105 16 sequences sequence NNS cord-269720-o81j3d1j 105 17 from from IN cord-269720-o81j3d1j 105 18 the the DT cord-269720-o81j3d1j 105 19 different different JJ cord-269720-o81j3d1j 105 20 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 105 21 , , , cord-269720-o81j3d1j 105 22 except except IN cord-269720-o81j3d1j 105 23 at at IN cord-269720-o81j3d1j 105 24 the the DT cord-269720-o81j3d1j 105 25 3 3 CD cord-269720-o81j3d1j 105 26 ' ' '' cord-269720-o81j3d1j 105 27 ends end NNS cord-269720-o81j3d1j 105 28 involving involve VBG cord-269720-o81j3d1j 105 29 the the DT cord-269720-o81j3d1j 105 30 consensus consensus NN cord-269720-o81j3d1j 105 31 sequences sequence NNS cord-269720-o81j3d1j 105 32 . . . cord-269720-o81j3d1j 106 1 In in IN cord-269720-o81j3d1j 106 2 order order NN cord-269720-o81j3d1j 106 3 to to TO cord-269720-o81j3d1j 106 4 identify identify VB cord-269720-o81j3d1j 106 5 any any DT cord-269720-o81j3d1j 106 6 potential potential JJ cord-269720-o81j3d1j 106 7 similarities similarity NNS cord-269720-o81j3d1j 106 8 in in IN cord-269720-o81j3d1j 106 9 these these DT cord-269720-o81j3d1j 106 10 sequences sequence NNS cord-269720-o81j3d1j 106 11 , , , cord-269720-o81j3d1j 106 12 the the DT cord-269720-o81j3d1j 106 13 secondary secondary JJ cord-269720-o81j3d1j 106 14 structure structure NN cord-269720-o81j3d1j 106 15 of of IN cord-269720-o81j3d1j 106 16 the the DT cord-269720-o81j3d1j 106 17 RNA RNA NNP cord-269720-o81j3d1j 106 18 sequences sequence NNS cord-269720-o81j3d1j 106 19 in in IN cord-269720-o81j3d1j 106 20 Fig Fig NNP cord-269720-o81j3d1j 106 21 . . . cord-269720-o81j3d1j 107 1 5 5 CD cord-269720-o81j3d1j 107 2 were be VBD cord-269720-o81j3d1j 107 3 analyzed analyze VBN cord-269720-o81j3d1j 107 4 . . . cord-269720-o81j3d1j 108 1 Potential potential JJ cord-269720-o81j3d1j 108 2 secondary secondary JJ cord-269720-o81j3d1j 108 3 structures structure NNS cord-269720-o81j3d1j 108 4 of of IN cord-269720-o81j3d1j 108 5 the the DT cord-269720-o81j3d1j 108 6 leader leader NN cord-269720-o81j3d1j 108 7 RNA RNA NNP cord-269720-o81j3d1j 108 8 sequences sequence NNS cord-269720-o81j3d1j 108 9 were be VBD cord-269720-o81j3d1j 108 10 determined determine VBN cord-269720-o81j3d1j 108 11 using use VBG cord-269720-o81j3d1j 108 12 the the DT cord-269720-o81j3d1j 108 13 computer computer NN cord-269720-o81j3d1j 108 14 program program NN cord-269720-o81j3d1j 108 15 FOLD fold VB cord-269720-o81j3d1j 108 16 ( ( -LRB- cord-269720-o81j3d1j 108 17 27 27 CD cord-269720-o81j3d1j 108 18 ) ) -RRB- cord-269720-o81j3d1j 108 19 from from IN cord-269720-o81j3d1j 108 20 the the DT cord-269720-o81j3d1j 108 21 UWGCG UWGCG NNP cord-269720-o81j3d1j 108 22 DNA dna NN cord-269720-o81j3d1j 108 23 analysis analysis NN cord-269720-o81j3d1j 108 24 programs program NNS cord-269720-o81j3d1j 108 25 ( ( -LRB- cord-269720-o81j3d1j 108 26 28 28 CD cord-269720-o81j3d1j 108 27 ) ) -RRB- cord-269720-o81j3d1j 108 28 . . . cord-269720-o81j3d1j 109 1 The the DT cord-269720-o81j3d1j 109 2 coordinates coordinate NNS cord-269720-o81j3d1j 109 3 determined determine VBN cord-269720-o81j3d1j 109 4 by by IN cord-269720-o81j3d1j 109 5 the the DT cord-269720-o81j3d1j 109 6 FOLD FOLD NNP cord-269720-o81j3d1j 109 7 program program NN cord-269720-o81j3d1j 109 8 were be VBD cord-269720-o81j3d1j 109 9 displayed display VBN cord-269720-o81j3d1j 109 10 graphically graphically RB cord-269720-o81j3d1j 109 11 using use VBG cord-269720-o81j3d1j 109 12 the the DT cord-269720-o81j3d1j 109 13 UWGCG UWGCG NNP cord-269720-o81j3d1j 109 14 program program NN cord-269720-o81j3d1j 109 15 SQUIG SQUIG NNP cord-269720-o81j3d1j 109 16 - - HYPH cord-269720-o81j3d1j 109 17 GLES GLES NNP cord-269720-o81j3d1j 109 18 . . . cord-269720-o81j3d1j 110 1 The the DT cord-269720-o81j3d1j 110 2 potential potential JJ cord-269720-o81j3d1j 110 3 secondary secondary JJ cord-269720-o81j3d1j 110 4 structures structure NNS cord-269720-o81j3d1j 110 5 obtained obtain VBN cord-269720-o81j3d1j 110 6 were be VBD cord-269720-o81j3d1j 110 7 compared compare VBN cord-269720-o81j3d1j 110 8 and and CC cord-269720-o81j3d1j 110 9 , , , cord-269720-o81j3d1j 110 10 as as IN cord-269720-o81j3d1j 110 11 can can MD cord-269720-o81j3d1j 110 12 be be VB cord-269720-o81j3d1j 110 13 seen see VBN cord-269720-o81j3d1j 110 14 from from IN cord-269720-o81j3d1j 110 15 Fig Fig NNP cord-269720-o81j3d1j 110 16 . . . cord-269720-o81j3d1j 111 1 6 6 CD cord-269720-o81j3d1j 111 2 , , , cord-269720-o81j3d1j 111 3 the the DT cord-269720-o81j3d1j 111 4 overall overall JJ cord-269720-o81j3d1j 111 5 shape shape NN cord-269720-o81j3d1j 111 6 of of IN cord-269720-o81j3d1j 111 7 these these DT cord-269720-o81j3d1j 111 8 sequences sequence NNS cord-269720-o81j3d1j 111 9 are be VBP cord-269720-o81j3d1j 111 10 very very RB cord-269720-o81j3d1j 111 11 similar similar JJ cord-269720-o81j3d1j 111 12 , , , cord-269720-o81j3d1j 111 13 except except IN cord-269720-o81j3d1j 111 14 for for IN cord-269720-o81j3d1j 111 15 the the DT cord-269720-o81j3d1j 111 16 avian avian JJ cord-269720-o81j3d1j 111 17 coronavirus coronavirus NN cord-269720-o81j3d1j 111 18 IBV IBV NNP cord-269720-o81j3d1j 111 19 . . . cord-269720-o81j3d1j 112 1 All all PDT cord-269720-o81j3d1j 112 2 the the DT cord-269720-o81j3d1j 112 3 molecules molecule NNS cord-269720-o81j3d1j 112 4 appear appear VBP cord-269720-o81j3d1j 112 5 to to TO cord-269720-o81j3d1j 112 6 be be VB cord-269720-o81j3d1j 112 7 composed compose VBN cord-269720-o81j3d1j 112 8 of of IN cord-269720-o81j3d1j 112 9 two two CD cord-269720-o81j3d1j 112 10 stem stem NN cord-269720-o81j3d1j 112 11 - - HYPH cord-269720-o81j3d1j 112 12 loop loop NN cord-269720-o81j3d1j 112 13 structures structure NNS cord-269720-o81j3d1j 112 14 . . . cord-269720-o81j3d1j 113 1 The the DT cord-269720-o81j3d1j 113 2 two two CD cord-269720-o81j3d1j 113 3 MHV MHV NNP cord-269720-o81j3d1j 113 4 molecules molecule NNS cord-269720-o81j3d1j 113 5 are be VBP cord-269720-o81j3d1j 113 6 very very RB cord-269720-o81j3d1j 113 7 similar similar JJ cord-269720-o81j3d1j 113 8 in in IN cord-269720-o81j3d1j 113 9 shape shape NN cord-269720-o81j3d1j 113 10 and and CC cord-269720-o81j3d1j 113 11 , , , cord-269720-o81j3d1j 113 12 as as IN cord-269720-o81j3d1j 113 13 seen see VBN cord-269720-o81j3d1j 113 14 from from IN cord-269720-o81j3d1j 113 15 Fig Fig NNP cord-269720-o81j3d1j 113 16 . . . cord-269720-o81j3d1j 114 1 5 5 CD cord-269720-o81j3d1j 114 2 and and CC cord-269720-o81j3d1j 114 3 Table Table NNP cord-269720-o81j3d1j 114 4 1 1 CD cord-269720-o81j3d1j 114 5 , , , cord-269720-o81j3d1j 114 6 are be VBP cord-269720-o81j3d1j 114 7 very very RB cord-269720-o81j3d1j 114 8 homologous homologous JJ cord-269720-o81j3d1j 114 9 , , , cord-269720-o81j3d1j 114 10 91 91 CD cord-269720-o81j3d1j 114 11 % % NN cord-269720-o81j3d1j 114 12 , , , cord-269720-o81j3d1j 114 13 at at IN cord-269720-o81j3d1j 114 14 base base NN cord-269720-o81j3d1j 114 15 sequence sequence NN cord-269720-o81j3d1j 114 16 . . . cord-269720-o81j3d1j 115 1 The the DT cord-269720-o81j3d1j 115 2 secondary secondary JJ cord-269720-o81j3d1j 115 3 structures structure NNS cord-269720-o81j3d1j 115 4 of of IN cord-269720-o81j3d1j 115 5 the the DT cord-269720-o81j3d1j 115 6 coronavirus coronavirus NN cord-269720-o81j3d1j 115 7 leader leader NN cord-269720-o81j3d1j 115 8 RNA RNA NNP cord-269720-o81j3d1j 115 9 sequences sequence NNS cord-269720-o81j3d1j 115 10 are be VBP cord-269720-o81j3d1j 115 11 probably probably RB cord-269720-o81j3d1j 115 12 influenced influence VBN cord-269720-o81j3d1j 115 13 by by IN cord-269720-o81j3d1j 115 14 their -PRON- PRP$ cord-269720-o81j3d1j 115 15 biological biological JJ cord-269720-o81j3d1j 115 16 function function NN cord-269720-o81j3d1j 115 17 , , , cord-269720-o81j3d1j 115 18 which which WDT cord-269720-o81j3d1j 115 19 results result VBZ cord-269720-o81j3d1j 115 20 in in IN cord-269720-o81j3d1j 115 21 the the DT cord-269720-o81j3d1j 115 22 similarity similarity NN cord-269720-o81j3d1j 115 23 of of IN cord-269720-o81j3d1j 115 24 these these DT cord-269720-o81j3d1j 115 25 potential potential JJ cord-269720-o81j3d1j 115 26 structures structure NNS cord-269720-o81j3d1j 115 27 . . . cord-269720-o81j3d1j 116 1 This this DT cord-269720-o81j3d1j 116 2 paper paper NN cord-269720-o81j3d1j 116 3 presents present VBZ cord-269720-o81j3d1j 116 4 evidence evidence NN cord-269720-o81j3d1j 116 5 that that IN cord-269720-o81j3d1j 116 6 the the DT cord-269720-o81j3d1j 116 7 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 116 8 mRNA mRNA NNP cord-269720-o81j3d1j 116 9 species specie NNS cord-269720-o81j3d1j 116 10 of of IN cord-269720-o81j3d1j 116 11 TGEV TGEV NNS cord-269720-o81j3d1j 116 12 and and CC cord-269720-o81j3d1j 116 13 the the DT cord-269720-o81j3d1j 116 14 closely closely RB cord-269720-o81j3d1j 116 15 related related JJ cord-269720-o81j3d1j 116 16 porcine porcine JJ cord-269720-o81j3d1j 116 17 respiratory respiratory JJ cord-269720-o81j3d1j 116 18 variant variant NN cord-269720-o81j3d1j 116 19 of of IN cord-269720-o81j3d1j 116 20 TGEV TGEV NNP cord-269720-o81j3d1j 116 21 , , , cord-269720-o81j3d1j 116 22 PRCV PRCV NNP cord-269720-o81j3d1j 116 23 , , , cord-269720-o81j3d1j 116 24 contain contain VBP cord-269720-o81j3d1j 116 25 an an DT cord-269720-o81j3d1j 116 26 identical identical JJ cord-269720-o81j3d1j 116 27 leader leader NN cord-269720-o81j3d1j 116 28 RNA RNA NNP cord-269720-o81j3d1j 116 29 sequence sequence NN cord-269720-o81j3d1j 116 30 of of IN cord-269720-o81j3d1j 116 31 about about RB cord-269720-o81j3d1j 116 32 91 91 CD cord-269720-o81j3d1j 116 33 nucleotides nucleotide NNS cord-269720-o81j3d1j 116 34 . . . cord-269720-o81j3d1j 117 1 Sequencing sequencing NN cord-269720-o81j3d1j 117 2 studies study NNS cord-269720-o81j3d1j 117 3 on on IN cord-269720-o81j3d1j 117 4 TGEV TGEV NNP cord-269720-o81j3d1j 117 5 have have VBP cord-269720-o81j3d1j 117 6 shown show VBN cord-269720-o81j3d1j 117 7 that that IN cord-269720-o81j3d1j 117 8 the the DT cord-269720-o81j3d1j 117 9 heptameric heptameric NN cord-269720-o81j3d1j 117 10 sequence sequence NN cord-269720-o81j3d1j 117 11 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 117 12 occurs occur VBZ cord-269720-o81j3d1j 117 13 on on IN cord-269720-o81j3d1j 117 14 the the DT cord-269720-o81j3d1j 117 15 genome genome NN cord-269720-o81j3d1j 117 16 upstream upstream RB cord-269720-o81j3d1j 117 17 of of IN cord-269720-o81j3d1j 117 18 the the DT cord-269720-o81j3d1j 117 19 genes gene NNS cord-269720-o81j3d1j 117 20 and and CC cord-269720-o81j3d1j 117 21 is be VBZ cord-269720-o81j3d1j 117 22 believed believe VBN cord-269720-o81j3d1j 117 23 to to TO cord-269720-o81j3d1j 117 24 be be VB cord-269720-o81j3d1j 117 25 the the DT cord-269720-o81j3d1j 117 26 binding binding NN cord-269720-o81j3d1j 117 27 site site NN cord-269720-o81j3d1j 117 28 for for IN cord-269720-o81j3d1j 117 29 the the DT cord-269720-o81j3d1j 117 30 leader leader NN cord-269720-o81j3d1j 117 31 of of IN cord-269720-o81j3d1j 117 32 the the DT cord-269720-o81j3d1j 117 33 genomic genomic JJ cord-269720-o81j3d1j 117 34 RNA RNA NNP cord-269720-o81j3d1j 117 35 . . . cord-269720-o81j3d1j 118 1 This this DT cord-269720-o81j3d1j 118 2 mechanism mechanism NN cord-269720-o81j3d1j 118 3 has have VBZ cord-269720-o81j3d1j 118 4 been be VBN cord-269720-o81j3d1j 118 5 termed term VBN cord-269720-o81j3d1j 118 6 leader leader NN cord-269720-o81j3d1j 118 7 - - HYPH cord-269720-o81j3d1j 118 8 primed prime VBN cord-269720-o81j3d1j 118 9 transcription transcription NN cord-269720-o81j3d1j 118 10 and and CC cord-269720-o81j3d1j 118 11 involves involve VBZ cord-269720-o81j3d1j 118 12 not not RB cord-269720-o81j3d1j 118 13 only only RB cord-269720-o81j3d1j 118 14 the the DT cord-269720-o81j3d1j 118 15 leader leader NN cord-269720-o81j3d1j 118 16 RNA RNA NNP cord-269720-o81j3d1j 118 17 primer primer NN cord-269720-o81j3d1j 118 18 , , , cord-269720-o81j3d1j 118 19 but but CC cord-269720-o81j3d1j 118 20 also also RB cord-269720-o81j3d1j 118 21 consensus consensus NN cord-269720-o81j3d1j 118 22 sequences sequence NNS cord-269720-o81j3d1j 118 23 along along IN cord-269720-o81j3d1j 118 24 the the DT cord-269720-o81j3d1j 118 25 genome genome NN cord-269720-o81j3d1j 118 26 found find VBN cord-269720-o81j3d1j 118 27 upstream upstream RB cord-269720-o81j3d1j 118 28 of of IN cord-269720-o81j3d1j 118 29 the the DT cord-269720-o81j3d1j 118 30 genes gene NNS cord-269720-o81j3d1j 118 31 , , , cord-269720-o81j3d1j 118 32 which which WDT cord-269720-o81j3d1j 118 33 act act VBP cord-269720-o81j3d1j 118 34 as as IN cord-269720-o81j3d1j 118 35 binding bind VBG cord-269720-o81j3d1j 118 36 sites site NNS cord-269720-o81j3d1j 118 37 for for IN cord-269720-o81j3d1j 118 38 the the DT cord-269720-o81j3d1j 118 39 leader leader NN cord-269720-o81j3d1j 118 40 RNA RNA NNP cord-269720-o81j3d1j 118 41 primer primer NN cord-269720-o81j3d1j 118 42 . . . cord-269720-o81j3d1j 119 1 Comparison comparison NN cord-269720-o81j3d1j 119 2 of of IN cord-269720-o81j3d1j 119 3 TGEV TGEV NNP cord-269720-o81j3d1j 119 4 and and CC cord-269720-o81j3d1j 119 5 PRCV PRCV NNP cord-269720-o81j3d1j 119 6 viral viral JJ cord-269720-o81j3d1j 119 7 products product NNS cord-269720-o81j3d1j 119 8 has have VBZ cord-269720-o81j3d1j 119 9 shown show VBN cord-269720-o81j3d1j 119 10 very very RB cord-269720-o81j3d1j 119 11 little little JJ cord-269720-o81j3d1j 119 12 difference difference NN cord-269720-o81j3d1j 119 13 between between IN cord-269720-o81j3d1j 119 14 the the DT cord-269720-o81j3d1j 119 15 two two CD cord-269720-o81j3d1j 119 16 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 119 17 , , , cord-269720-o81j3d1j 119 18 and and CC cord-269720-o81j3d1j 119 19 until until IN cord-269720-o81j3d1j 119 20 recently recently RB cord-269720-o81j3d1j 119 21 is be VBZ cord-269720-o81j3d1j 119 22 was be VBD cord-269720-o81j3d1j 119 23 impossible impossible JJ cord-269720-o81j3d1j 119 24 to to TO cord-269720-o81j3d1j 119 25 differentiate differentiate VB cord-269720-o81j3d1j 119 26 between between IN cord-269720-o81j3d1j 119 27 the the DT cord-269720-o81j3d1j 119 28 two two CD cord-269720-o81j3d1j 119 29 viruses virus NNS cord-269720-o81j3d1j 119 30 using use VBG cord-269720-o81j3d1j 119 31 antisera antisera NN cord-269720-o81j3d1j 119 32 . . . cord-269720-o81j3d1j 120 1 PRCV PRCV NNP cord-269720-o81j3d1j 120 2 is be VBZ cord-269720-o81j3d1j 120 3 fully fully RB cord-269720-o81j3d1j 120 4 neutralized neutralize VBN cord-269720-o81j3d1j 120 5 by by IN cord-269720-o81j3d1j 120 6 antisera antisera NN cord-269720-o81j3d1j 120 7 prepared prepare VBN cord-269720-o81j3d1j 120 8 against against IN cord-269720-o81j3d1j 120 9 TGEV TGEV NNP cord-269720-o81j3d1j 120 10 , , , cord-269720-o81j3d1j 120 11 and and CC cord-269720-o81j3d1j 120 12 the the DT cord-269720-o81j3d1j 120 13 majority majority NN cord-269720-o81j3d1j 120 14 of of IN cord-269720-o81j3d1j 120 15 monoclonal monoclonal JJ cord-269720-o81j3d1j 120 16 antibodies antibody NNS cord-269720-o81j3d1j 120 17 ( ( -LRB- cord-269720-o81j3d1j 120 18 MAbs MAbs NNP cord-269720-o81j3d1j 120 19 ) ) -RRB- cord-269720-o81j3d1j 120 20 raised raise VBD cord-269720-o81j3d1j 120 21 against against IN cord-269720-o81j3d1j 120 22 TGEV TGEV NNP cord-269720-o81j3d1j 120 23 virion virion NN cord-269720-o81j3d1j 120 24 proteins protein NNS cord-269720-o81j3d1j 120 25 cross cross VBP cord-269720-o81j3d1j 120 26 - - VBP cord-269720-o81j3d1j 120 27 react react VBP cord-269720-o81j3d1j 120 28 with with IN cord-269720-o81j3d1j 120 29 PRCV PRCV NNP cord-269720-o81j3d1j 120 30 . . . cord-269720-o81j3d1j 121 1 However however RB cord-269720-o81j3d1j 121 2 , , , cord-269720-o81j3d1j 121 3 MAbs MAbs NNP cord-269720-o81j3d1j 121 4 , , , cord-269720-o81j3d1j 121 5 raised raise VBN cord-269720-o81j3d1j 121 6 against against IN cord-269720-o81j3d1j 121 7 antigenic antigenic JJ cord-269720-o81j3d1j 121 8 determinants determinant NNS cord-269720-o81j3d1j 121 9 of of IN cord-269720-o81j3d1j 121 10 the the DT cord-269720-o81j3d1j 121 11 spike spike NN cord-269720-o81j3d1j 121 12 protein protein NN cord-269720-o81j3d1j 121 13 from from IN cord-269720-o81j3d1j 121 14 either either CC cord-269720-o81j3d1j 121 15 the the DT cord-269720-o81j3d1j 121 16 virulent virulent JJ cord-269720-o81j3d1j 121 17 British british JJ cord-269720-o81j3d1j 121 18 isolate isolate VBP cord-269720-o81j3d1j 121 19 FS772/70 FS772/70 NNP cord-269720-o81j3d1j 121 20 ( ( -LRB- cord-269720-o81j3d1j 121 21 29 29 CD cord-269720-o81j3d1j 121 22 ) ) -RRB- cord-269720-o81j3d1j 121 23 or or CC cord-269720-o81j3d1j 121 24 the the DT cord-269720-o81j3d1j 121 25 avirulent avirulent NN cord-269720-o81j3d1j 121 26 Purdue Purdue NNP cord-269720-o81j3d1j 121 27 strain strain NN cord-269720-o81j3d1j 121 28 of of IN cord-269720-o81j3d1j 121 29 TGEV TGEV NNP cord-269720-o81j3d1j 121 30 ( ( -LRB- cord-269720-o81j3d1j 121 31 30 30 CD cord-269720-o81j3d1j 121 32 ) ) -RRB- cord-269720-o81j3d1j 121 33 have have VBP cord-269720-o81j3d1j 121 34 been be VBN cord-269720-o81j3d1j 121 35 identified identify VBN cord-269720-o81j3d1j 121 36 that that WDT cord-269720-o81j3d1j 121 37 do do VBP cord-269720-o81j3d1j 121 38 not not RB cord-269720-o81j3d1j 121 39 recognize recognize VB cord-269720-o81j3d1j 121 40 PRCV PRCV NNP cord-269720-o81j3d1j 121 41 . . . cord-269720-o81j3d1j 122 1 These these DT cord-269720-o81j3d1j 122 2 observations observation NNS cord-269720-o81j3d1j 122 3 and and CC cord-269720-o81j3d1j 122 4 the the DT cord-269720-o81j3d1j 122 5 fact fact NN cord-269720-o81j3d1j 122 6 that that IN cord-269720-o81j3d1j 122 7 the the DT cord-269720-o81j3d1j 122 8 leader leader NN cord-269720-o81j3d1j 122 9 RNA RNA NNP cord-269720-o81j3d1j 122 10 sequences sequence NNS cord-269720-o81j3d1j 122 11 from from IN cord-269720-o81j3d1j 122 12 TGEV TGEV NNP cord-269720-o81j3d1j 122 13 and and CC cord-269720-o81j3d1j 122 14 PRCV PRCV NNPS cord-269720-o81j3d1j 122 15 are be VBP cord-269720-o81j3d1j 122 16 identical identical JJ cord-269720-o81j3d1j 122 17 supports support NNS cord-269720-o81j3d1j 122 18 the the DT cord-269720-o81j3d1j 122 19 evidence evidence NN cord-269720-o81j3d1j 122 20 that that IN cord-269720-o81j3d1j 122 21 the the DT cord-269720-o81j3d1j 122 22 two two CD cord-269720-o81j3d1j 122 23 viruses virus NNS cord-269720-o81j3d1j 122 24 are be VBP cord-269720-o81j3d1j 122 25 very very RB cord-269720-o81j3d1j 122 26 similar similar JJ cord-269720-o81j3d1j 122 27 and and CC cord-269720-o81j3d1j 122 28 that that IN cord-269720-o81j3d1j 122 29 PRCV PRCV NNP cord-269720-o81j3d1j 122 30 may may MD cord-269720-o81j3d1j 122 31 have have VB cord-269720-o81j3d1j 122 32 evolved evolve VBN cord-269720-o81j3d1j 122 33 as as IN cord-269720-o81j3d1j 122 34 a a DT cord-269720-o81j3d1j 122 35 TGEV TGEV NNP cord-269720-o81j3d1j 122 36 variant variant NN cord-269720-o81j3d1j 122 37 . . . cord-269720-o81j3d1j 123 1 Comparison comparison NN cord-269720-o81j3d1j 123 2 of of IN cord-269720-o81j3d1j 123 3 the the DT cord-269720-o81j3d1j 123 4 TGEV TGEV NNP cord-269720-o81j3d1j 123 5 leader leader NN cord-269720-o81j3d1j 123 6 RNA RNA NNP cord-269720-o81j3d1j 123 7 sequence sequence NN cord-269720-o81j3d1j 123 8 with with IN cord-269720-o81j3d1j 123 9 the the DT cord-269720-o81j3d1j 123 10 genomic genomic JJ cord-269720-o81j3d1j 123 11 sequence sequence NN cord-269720-o81j3d1j 123 12 upstream upstream RB cord-269720-o81j3d1j 123 13 of of IN cord-269720-o81j3d1j 123 14 the the DT cord-269720-o81j3d1j 123 15 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 123 16 indicates indicate VBZ cord-269720-o81j3d1j 123 17 that that IN cord-269720-o81j3d1j 123 18 the the DT cord-269720-o81j3d1j 123 19 length length NN cord-269720-o81j3d1j 123 20 of of IN cord-269720-o81j3d1j 123 21 the the DT cord-269720-o81j3d1j 123 22 unique unique JJ cord-269720-o81j3d1j 123 23 sequence sequence NN cord-269720-o81j3d1j 123 24 of of IN cord-269720-o81j3d1j 123 25 the the DT cord-269720-o81j3d1j 123 26 leader leader NN cord-269720-o81j3d1j 123 27 sequence sequence NN cord-269720-o81j3d1j 123 28 is be VBZ cord-269720-o81j3d1j 123 29 91 91 CD cord-269720-o81j3d1j 123 30 nucleotides nucleotide NNS cord-269720-o81j3d1j 123 31 . . . cord-269720-o81j3d1j 124 1 The the DT cord-269720-o81j3d1j 124 2 point point NN cord-269720-o81j3d1j 124 3 of of IN cord-269720-o81j3d1j 124 4 divergence divergence NN cord-269720-o81j3d1j 124 5 is be VBZ cord-269720-o81j3d1j 124 6 two two CD cord-269720-o81j3d1j 124 7 bases basis NNS cord-269720-o81j3d1j 124 8 upstream upstream RB cord-269720-o81j3d1j 124 9 of of IN cord-269720-o81j3d1j 124 10 the the DT cord-269720-o81j3d1j 124 11 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 124 12 sequence sequence NN cord-269720-o81j3d1j 124 13 , , , cord-269720-o81j3d1j 124 14 supporting support VBG cord-269720-o81j3d1j 124 15 the the DT cord-269720-o81j3d1j 124 16 evidence evidence NN cord-269720-o81j3d1j 124 17 that that IN cord-269720-o81j3d1j 124 18 the the DT cord-269720-o81j3d1j 124 19 TGEV TGEV NNP cord-269720-o81j3d1j 124 20 RNA RNA NNP cord-269720-o81j3d1j 124 21 polymerase polymerase NN cord-269720-o81j3d1j 124 22 - - HYPH cord-269720-o81j3d1j 124 23 leader leader NN cord-269720-o81j3d1j 124 24 complex complex JJ cord-269720-o81j3d1j 124 25 binding binding NN cord-269720-o81j3d1j 124 26 site site NN cord-269720-o81j3d1j 124 27 is be VBZ cord-269720-o81j3d1j 124 28 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 124 29 . . . cord-269720-o81j3d1j 125 1 Four four CD cord-269720-o81j3d1j 125 2 out out IN cord-269720-o81j3d1j 125 3 of of IN cord-269720-o81j3d1j 125 4 the the DT cord-269720-o81j3d1j 125 5 six six CD cord-269720-o81j3d1j 125 6 mRNA mrna NN cord-269720-o81j3d1j 125 7 species specie NNS cord-269720-o81j3d1j 125 8 from from IN cord-269720-o81j3d1j 125 9 the the DT cord-269720-o81j3d1j 125 10 FS772/70 FS772/70 NNP cord-269720-o81j3d1j 125 11 strain strain NN cord-269720-o81j3d1j 125 12 of of IN cord-269720-o81j3d1j 125 13 TGEV TGEV NNP cord-269720-o81j3d1j 125 14 have have VBP cord-269720-o81j3d1j 125 15 the the DT cord-269720-o81j3d1j 125 16 sequence sequence NN cord-269720-o81j3d1j 125 17 AACTAAAC AACTAAAC NNP cord-269720-o81j3d1j 125 18 , , , cord-269720-o81j3d1j 125 19 and and CC cord-269720-o81j3d1j 125 20 the the DT cord-269720-o81j3d1j 125 21 5'-end 5'-end CD cord-269720-o81j3d1j 125 22 adenosine adenosine NN cord-269720-o81j3d1j 125 23 residue residue NN cord-269720-o81j3d1j 125 24 is be VBZ cord-269720-o81j3d1j 125 25 the the DT cord-269720-o81j3d1j 125 26 next next JJ cord-269720-o81j3d1j 125 27 base base NN cord-269720-o81j3d1j 125 28 down down RP cord-269720-o81j3d1j 125 29 from from IN cord-269720-o81j3d1j 125 30 the the DT cord-269720-o81j3d1j 125 31 divergence divergence NN cord-269720-o81j3d1j 125 32 point point NN cord-269720-o81j3d1j 125 33 in in IN cord-269720-o81j3d1j 125 34 the the DT cord-269720-o81j3d1j 125 35 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 126 1 mRNA mRNA NNP cord-269720-o81j3d1j 127 1 ( ( -LRB- cord-269720-o81j3d1j 127 2 Fig Fig NNP cord-269720-o81j3d1j 127 3 . . NNP cord-269720-o81j3d1j 127 4 2 2 CD cord-269720-o81j3d1j 127 5 ) ) -RRB- cord-269720-o81j3d1j 127 6 . . . cord-269720-o81j3d1j 128 1 The the DT cord-269720-o81j3d1j 128 2 differences difference NNS cord-269720-o81j3d1j 128 3 in in IN cord-269720-o81j3d1j 128 4 the the DT cord-269720-o81j3d1j 128 5 homologies homology NNS cord-269720-o81j3d1j 128 6 between between IN cord-269720-o81j3d1j 128 7 the the DT cord-269720-o81j3d1j 128 8 leader leader NN cord-269720-o81j3d1j 128 9 RNA RNA NNP cord-269720-o81j3d1j 128 10 and and CC cord-269720-o81j3d1j 128 11 sequences sequence NNS cord-269720-o81j3d1j 128 12 upstream upstream RB cord-269720-o81j3d1j 128 13 of of IN cord-269720-o81j3d1j 128 14 the the DT cord-269720-o81j3d1j 128 15 consensus consensus NN cord-269720-o81j3d1j 128 16 sequence sequence NN cord-269720-o81j3d1j 128 17 on on IN cord-269720-o81j3d1j 128 18 the the DT cord-269720-o81j3d1j 128 19 genomic genomic JJ cord-269720-o81j3d1j 128 20 RNA RNA NNP cord-269720-o81j3d1j 128 21 may may MD cord-269720-o81j3d1j 128 22 play play VB cord-269720-o81j3d1j 128 23 a a DT cord-269720-o81j3d1j 128 24 role role NN cord-269720-o81j3d1j 128 25 in in IN cord-269720-o81j3d1j 128 26 the the DT cord-269720-o81j3d1j 128 27 levels level NNS cord-269720-o81j3d1j 128 28 of of IN cord-269720-o81j3d1j 128 29 transcription transcription NN cord-269720-o81j3d1j 128 30 of of IN cord-269720-o81j3d1j 128 31 a a DT cord-269720-o81j3d1j 128 32 particular particular JJ cord-269720-o81j3d1j 128 33 mRNA mRNA NNP cord-269720-o81j3d1j 128 34 species specie NNS cord-269720-o81j3d1j 128 35 . . . cord-269720-o81j3d1j 129 1 The the DT cord-269720-o81j3d1j 129 2 mRNA mRNA NNP cord-269720-o81j3d1j 129 3 species specie NNS cord-269720-o81j3d1j 129 4 of of IN cord-269720-o81j3d1j 129 5 3.0 3.0 CD cord-269720-o81j3d1j 129 6 kb kb NNP cord-269720-o81j3d1j 129 7 has have VBZ cord-269720-o81j3d1j 129 8 been be VBN cord-269720-o81j3d1j 129 9 shown show VBN cord-269720-o81j3d1j 129 10 to to TO cord-269720-o81j3d1j 129 11 have have VB cord-269720-o81j3d1j 129 12 an an DT cord-269720-o81j3d1j 129 13 open open JJ cord-269720-o81j3d1j 129 14 reading reading NN cord-269720-o81j3d1j 129 15 frame frame NN cord-269720-o81j3d1j 129 16 at at IN cord-269720-o81j3d1j 129 17 the the DT cord-269720-o81j3d1j 129 18 5 5 CD cord-269720-o81j3d1j 129 19 ' ' NN cord-269720-o81j3d1j 129 20 end end NN cord-269720-o81j3d1j 129 21 encoding encode VBG cord-269720-o81j3d1j 129 22 a a DT cord-269720-o81j3d1j 129 23 potential potential JJ cord-269720-o81j3d1j 129 24 polypeptide polypeptide NN cord-269720-o81j3d1j 129 25 of of IN cord-269720-o81j3d1j 129 26 M M NNP cord-269720-o81j3d1j 129 27 r r NN cord-269720-o81j3d1j 129 28 9200 9200 CD cord-269720-o81j3d1j 129 29 ( ( -LRB- cord-269720-o81j3d1j 129 30 17 17 CD cord-269720-o81j3d1j 129 31 ) ) -RRB- cord-269720-o81j3d1j 129 32 . . . cord-269720-o81j3d1j 130 1 This this DT cord-269720-o81j3d1j 130 2 particular particular JJ cord-269720-o81j3d1j 130 3 mRNA mrna NN cord-269720-o81j3d1j 130 4 does do VBZ cord-269720-o81j3d1j 130 5 not not RB cord-269720-o81j3d1j 130 6 have have VB cord-269720-o81j3d1j 130 7 the the DT cord-269720-o81j3d1j 130 8 heptameric heptameric JJ cord-269720-o81j3d1j 130 9 consensus consensus NN cord-269720-o81j3d1j 130 10 sequence sequence NN cord-269720-o81j3d1j 130 11 but but CC cord-269720-o81j3d1j 130 12 has have VBZ cord-269720-o81j3d1j 130 13 the the DT cord-269720-o81j3d1j 130 14 hexameric hexameric JJ cord-269720-o81j3d1j 130 15 CTAAAC ctaaac NN cord-269720-o81j3d1j 130 16 sequence sequence NN cord-269720-o81j3d1j 130 17 , , , cord-269720-o81j3d1j 130 18 and and CC cord-269720-o81j3d1j 130 19 it -PRON- PRP cord-269720-o81j3d1j 130 20 is be VBZ cord-269720-o81j3d1j 130 21 interesting interesting JJ cord-269720-o81j3d1j 130 22 to to TO cord-269720-o81j3d1j 130 23 note note VB cord-269720-o81j3d1j 130 24 that that IN cord-269720-o81j3d1j 130 25 it -PRON- PRP cord-269720-o81j3d1j 130 26 is be VBZ cord-269720-o81j3d1j 130 27 the the DT cord-269720-o81j3d1j 130 28 least least JJS cord-269720-o81j3d1j 130 29 abundant abundant JJ cord-269720-o81j3d1j 130 30 TGEV TGEV NNP cord-269720-o81j3d1j 130 31 mRNA mrna NN cord-269720-o81j3d1j 130 32 species specie NNS cord-269720-o81j3d1j 130 33 ( ( -LRB- cord-269720-o81j3d1j 130 34 observed observe VBN cord-269720-o81j3d1j 130 35 from from IN cord-269720-o81j3d1j 130 36 TGEV TGEV NNP cord-269720-o81j3d1j 130 37 mRNA mRNA NNP cord-269720-o81j3d1j 130 38 in in IN cord-269720-o81j3d1j 130 39 total total JJ cord-269720-o81j3d1j 130 40 cell cell NN cord-269720-o81j3d1j 130 41 lysates lysate NNS cord-269720-o81j3d1j 130 42 ) ) -RRB- cord-269720-o81j3d1j 130 43 . . . cord-269720-o81j3d1j 131 1 Hybridization hybridization NN cord-269720-o81j3d1j 131 2 of of IN cord-269720-o81j3d1j 131 3 oligo oligo NN cord-269720-o81j3d1j 131 4 58 58 CD cord-269720-o81j3d1j 131 5 to to IN cord-269720-o81j3d1j 131 6 the the DT cord-269720-o81j3d1j 131 7 3.0-kb 3.0-kb CD cord-269720-o81j3d1j 131 8 mRNA mrna NN cord-269720-o81j3d1j 131 9 species specie NNS cord-269720-o81j3d1j 131 10 showed show VBD cord-269720-o81j3d1j 131 11 that that IN cord-269720-o81j3d1j 131 12 this this DT cord-269720-o81j3d1j 131 13 species species NN cord-269720-o81j3d1j 131 14 does do VBZ cord-269720-o81j3d1j 131 15 contain contain VB cord-269720-o81j3d1j 131 16 the the DT cord-269720-o81j3d1j 131 17 TGEV TGEV NNP cord-269720-o81j3d1j 131 18 leader leader NN cord-269720-o81j3d1j 131 19 RNA RNA NNP cord-269720-o81j3d1j 131 20 , , , cord-269720-o81j3d1j 131 21 confirming confirm VBG cord-269720-o81j3d1j 131 22 that that IN cord-269720-o81j3d1j 131 23 it -PRON- PRP cord-269720-o81j3d1j 131 24 is be VBZ cord-269720-o81j3d1j 131 25 a a DT cord-269720-o81j3d1j 131 26 true true JJ cord-269720-o81j3d1j 131 27 mRNA mRNA NNP cord-269720-o81j3d1j 131 28 species specie NNS cord-269720-o81j3d1j 131 29 , , , cord-269720-o81j3d1j 131 30 even even RB cord-269720-o81j3d1j 131 31 though though IN cord-269720-o81j3d1j 131 32 it -PRON- PRP cord-269720-o81j3d1j 131 33 is be VBZ cord-269720-o81j3d1j 131 34 the the DT cord-269720-o81j3d1j 131 35 only only JJ cord-269720-o81j3d1j 131 36 TGEV TGEV NNP cord-269720-o81j3d1j 131 37 species specie NNS cord-269720-o81j3d1j 131 38 not not RB cord-269720-o81j3d1j 131 39 to to TO cord-269720-o81j3d1j 131 40 have have VB cord-269720-o81j3d1j 131 41 the the DT cord-269720-o81j3d1j 131 42 heptameric heptameric JJ cord-269720-o81j3d1j 131 43 consensus consensus NN cord-269720-o81j3d1j 131 44 sequence sequence NN cord-269720-o81j3d1j 131 45 . . . cord-269720-o81j3d1j 132 1 Comparison comparison NN cord-269720-o81j3d1j 132 2 of of IN cord-269720-o81j3d1j 132 3 the the DT cord-269720-o81j3d1j 132 4 seven seven CD cord-269720-o81j3d1j 132 5 coronavirus coronavirus NN cord-269720-o81j3d1j 132 6 leader leader NN cord-269720-o81j3d1j 132 7 RNA RNA NNP cord-269720-o81j3d1j 132 8 sequences sequence NNS cord-269720-o81j3d1j 132 9 against against IN cord-269720-o81j3d1j 132 10 each each DT cord-269720-o81j3d1j 132 11 other other JJ cord-269720-o81j3d1j 132 12 identified identify VBD cord-269720-o81j3d1j 132 13 three three CD cord-269720-o81j3d1j 132 14 groups group NNS cord-269720-o81j3d1j 132 15 ( ( -LRB- cord-269720-o81j3d1j 132 16 Table table NN cord-269720-o81j3d1j 132 17 1 1 CD cord-269720-o81j3d1j 132 18 ) ) -RRB- cord-269720-o81j3d1j 132 19 : : : cord-269720-o81j3d1j 133 1 non non JJ cord-269720-o81j3d1j 133 2 - - JJ cord-269720-o81j3d1j 133 3 serologically serologically RB cord-269720-o81j3d1j 133 4 related relate VBN cord-269720-o81j3d1j 133 5 viruses virus NNS cord-269720-o81j3d1j 133 6 had have VBD cord-269720-o81j3d1j 133 7 about about RB cord-269720-o81j3d1j 133 8 35 35 CD cord-269720-o81j3d1j 133 9 - - SYM cord-269720-o81j3d1j 133 10 40 40 CD cord-269720-o81j3d1j 133 11 % % NN cord-269720-o81j3d1j 133 12 homology homology NN cord-269720-o81j3d1j 133 13 ; ; : cord-269720-o81j3d1j 133 14 serologically serologically RB cord-269720-o81j3d1j 133 15 related relate VBN cord-269720-o81j3d1j 133 16 viruses virus NNS cord-269720-o81j3d1j 133 17 had have VBD cord-269720-o81j3d1j 133 18 about about RB cord-269720-o81j3d1j 133 19 60 60 CD cord-269720-o81j3d1j 133 20 % % NN cord-269720-o81j3d1j 133 21 homology homology NN cord-269720-o81j3d1j 133 22 ; ; , cord-269720-o81j3d1j 133 23 viral viral JJ cord-269720-o81j3d1j 133 24 strains strain NNS cord-269720-o81j3d1j 133 25 had have VBD cord-269720-o81j3d1j 133 26 about about RB cord-269720-o81j3d1j 133 27 90 90 CD cord-269720-o81j3d1j 133 28 - - SYM cord-269720-o81j3d1j 133 29 100 100 CD cord-269720-o81j3d1j 133 30 % % NN cord-269720-o81j3d1j 133 31 homology homology NN cord-269720-o81j3d1j 133 32 . . . cord-269720-o81j3d1j 134 1 However however RB cord-269720-o81j3d1j 134 2 , , , cord-269720-o81j3d1j 134 3 TGEV TGEV NNP cord-269720-o81j3d1j 134 4 and and CC cord-269720-o81j3d1j 134 5 HCV HCV NNP cord-269720-o81j3d1j 134 6 ( ( -LRB- cord-269720-o81j3d1j 134 7 229E 229e CD cord-269720-o81j3d1j 134 8 ) ) -RRB- cord-269720-o81j3d1j 134 9 have have VBP cord-269720-o81j3d1j 134 10 been be VBN cord-269720-o81j3d1j 134 11 placed place VBN cord-269720-o81j3d1j 134 12 in in IN cord-269720-o81j3d1j 134 13 the the DT cord-269720-o81j3d1j 134 14 same same JJ cord-269720-o81j3d1j 134 15 serological serological JJ cord-269720-o81j3d1j 134 16 group group NN cord-269720-o81j3d1j 134 17 , , , cord-269720-o81j3d1j 134 18 but but CC cord-269720-o81j3d1j 134 19 have have VBP cord-269720-o81j3d1j 134 20 only only RB cord-269720-o81j3d1j 134 21 36 36 CD cord-269720-o81j3d1j 134 22 % % NN cord-269720-o81j3d1j 134 23 homology homology NN cord-269720-o81j3d1j 134 24 within within IN cord-269720-o81j3d1j 134 25 their -PRON- PRP$ cord-269720-o81j3d1j 134 26 leader leader NN cord-269720-o81j3d1j 134 27 RNA RNA NNP cord-269720-o81j3d1j 134 28 sequences sequence NNS cord-269720-o81j3d1j 134 29 , , , cord-269720-o81j3d1j 134 30 suggesting suggest VBG cord-269720-o81j3d1j 134 31 that that IN cord-269720-o81j3d1j 134 32 the the DT cord-269720-o81j3d1j 134 33 two two CD cord-269720-o81j3d1j 134 34 viruses virus NNS cord-269720-o81j3d1j 134 35 are be VBP cord-269720-o81j3d1j 134 36 not not RB cord-269720-o81j3d1j 134 37 particularly particularly RB cord-269720-o81j3d1j 134 38 related relate VBN cord-269720-o81j3d1j 134 39 . . . cord-269720-o81j3d1j 135 1 TGEV TGEV NNP cord-269720-o81j3d1j 135 2 and and CC cord-269720-o81j3d1j 135 3 HCV HCV NNP cord-269720-o81j3d1j 135 4 ( ( -LRB- cord-269720-o81j3d1j 135 5 229E 229e CD cord-269720-o81j3d1j 135 6 ) ) -RRB- cord-269720-o81j3d1j 135 7 have have VBP cord-269720-o81j3d1j 135 8 been be VBN cord-269720-o81j3d1j 135 9 shown show VBN cord-269720-o81j3d1j 135 10 to to TO cord-269720-o81j3d1j 135 11 have have VB cord-269720-o81j3d1j 135 12 46 46 CD cord-269720-o81j3d1j 135 13 % % NN cord-269720-o81j3d1j 135 14 homology homology NN cord-269720-o81j3d1j 135 15 at at IN cord-269720-o81j3d1j 135 16 the the DT cord-269720-o81j3d1j 135 17 amino amino NN cord-269720-o81j3d1j 135 18 acid acid NN cord-269720-o81j3d1j 135 19 level level NN cord-269720-o81j3d1j 135 20 within within IN cord-269720-o81j3d1j 135 21 their -PRON- PRP$ cord-269720-o81j3d1j 135 22 derived derive VBN cord-269720-o81j3d1j 135 23 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 135 24 sequences sequence NNS cord-269720-o81j3d1j 135 25 ( ( -LRB- cord-269720-o81j3d1j 135 26 31 31 CD cord-269720-o81j3d1j 135 27 ) ) -RRB- cord-269720-o81j3d1j 135 28 , , , cord-269720-o81j3d1j 135 29 whereas whereas IN cord-269720-o81j3d1j 135 30 the the DT cord-269720-o81j3d1j 135 31 homology homology NN cord-269720-o81j3d1j 135 32 between between IN cord-269720-o81j3d1j 135 33 the the DT cord-269720-o81j3d1j 135 34 derived derive VBN cord-269720-o81j3d1j 135 35 nucleoprotein nucleoprotein JJ cord-269720-o81j3d1j 135 36 amino amino NN cord-269720-o81j3d1j 135 37 acid acid NN cord-269720-o81j3d1j 135 38 sequences sequence NNS cord-269720-o81j3d1j 135 39 for for IN cord-269720-o81j3d1j 135 40 different different JJ cord-269720-o81j3d1j 135 41 viruses virus NNS cord-269720-o81j3d1j 135 42 within within IN cord-269720-o81j3d1j 135 43 the the DT cord-269720-o81j3d1j 135 44 MHV MHV NNP cord-269720-o81j3d1j 135 45 serological serological JJ cord-269720-o81j3d1j 135 46 group group NN cord-269720-o81j3d1j 135 47 are be VBP cord-269720-o81j3d1j 135 48 between between IN cord-269720-o81j3d1j 135 49 80 80 CD cord-269720-o81j3d1j 135 50 % % NN cord-269720-o81j3d1j 135 51 and and CC cord-269720-o81j3d1j 135 52 98 98 CD cord-269720-o81j3d1j 135 53 % % NN cord-269720-o81j3d1j 135 54 homology homology NN cord-269720-o81j3d1j 135 55 . . . cord-269720-o81j3d1j 136 1 This this DT cord-269720-o81j3d1j 136 2 indicates indicate VBZ cord-269720-o81j3d1j 136 3 that that IN cord-269720-o81j3d1j 136 4 the the DT cord-269720-o81j3d1j 136 5 serological serological JJ cord-269720-o81j3d1j 136 6 grouping grouping NN cord-269720-o81j3d1j 136 7 of of IN cord-269720-o81j3d1j 136 8 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 136 9 is be VBZ cord-269720-o81j3d1j 136 10 not not RB cord-269720-o81j3d1j 136 11 a a DT cord-269720-o81j3d1j 136 12 particularly particularly RB cord-269720-o81j3d1j 136 13 useful useful JJ cord-269720-o81j3d1j 136 14 test test NN cord-269720-o81j3d1j 136 15 , , , cord-269720-o81j3d1j 136 16 as as IN cord-269720-o81j3d1j 136 17 similar similar JJ cord-269720-o81j3d1j 136 18 epitopes epitope NNS cord-269720-o81j3d1j 136 19 may may MD cord-269720-o81j3d1j 136 20 exist exist VB cord-269720-o81j3d1j 136 21 on on IN cord-269720-o81j3d1j 136 22 the the DT cord-269720-o81j3d1j 136 23 viral viral JJ cord-269720-o81j3d1j 136 24 structural structural JJ cord-269720-o81j3d1j 136 25 proteins protein NNS cord-269720-o81j3d1j 136 26 . . . cord-269720-o81j3d1j 137 1 Comparisons comparison NNS cord-269720-o81j3d1j 137 2 of of IN cord-269720-o81j3d1j 137 3 nucleic nucleic NNS cord-269720-o81j3d1j 137 4 and and CC cord-269720-o81j3d1j 137 5 amino amino NN cord-269720-o81j3d1j 137 6 acid acid NN cord-269720-o81j3d1j 137 7 sequences sequence NNS cord-269720-o81j3d1j 137 8 from from IN cord-269720-o81j3d1j 137 9 the the DT cord-269720-o81j3d1j 137 10 viruses virus NNS cord-269720-o81j3d1j 137 11 will will MD cord-269720-o81j3d1j 137 12 provide provide VB cord-269720-o81j3d1j 137 13 a a DT cord-269720-o81j3d1j 137 14 more more RBR cord-269720-o81j3d1j 137 15 accurate accurate JJ cord-269720-o81j3d1j 137 16 method method NN cord-269720-o81j3d1j 137 17 for for IN cord-269720-o81j3d1j 137 18 grouping group VBG cord-269720-o81j3d1j 137 19 the the DT cord-269720-o81j3d1j 137 20 viruses virus NNS cord-269720-o81j3d1j 137 21 . . . cord-269720-o81j3d1j 138 1 It -PRON- PRP cord-269720-o81j3d1j 138 2 will will MD cord-269720-o81j3d1j 138 3 be be VB cord-269720-o81j3d1j 138 4 interesting interesting JJ cord-269720-o81j3d1j 138 5 to to TO cord-269720-o81j3d1j 138 6 compare compare VB cord-269720-o81j3d1j 138 7 the the DT cord-269720-o81j3d1j 138 8 leader leader NN cord-269720-o81j3d1j 138 9 sequences sequence NNS cord-269720-o81j3d1j 138 10 of of IN cord-269720-o81j3d1j 138 11 bovine bovine JJ cord-269720-o81j3d1j 138 12 coronavirus coronavirus NN cord-269720-o81j3d1j 138 13 ( ( -LRB- cord-269720-o81j3d1j 138 14 BCV BCV NNP cord-269720-o81j3d1j 138 15 ) ) -RRB- cord-269720-o81j3d1j 138 16 , , , cord-269720-o81j3d1j 138 17 which which WDT cord-269720-o81j3d1j 138 18 is be VBZ cord-269720-o81j3d1j 138 19 serologically serologically RB cord-269720-o81j3d1j 138 20 related relate VBN cord-269720-o81j3d1j 138 21 to to IN cord-269720-o81j3d1j 138 22 HCV HCV NNP cord-269720-o81j3d1j 138 23 ( ( -LRB- cord-269720-o81j3d1j 138 24 OC43 OC43 NNP cord-269720-o81j3d1j 138 25 ) ) -RRB- cord-269720-o81j3d1j 138 26 and and CC cord-269720-o81j3d1j 138 27 MHV MHV NNP cord-269720-o81j3d1j 138 28 ( ( -LRB- cord-269720-o81j3d1j 138 29 A59 A59 NNP cord-269720-o81j3d1j 138 30 and and CC cord-269720-o81j3d1j 138 31 JHM JHM NNP cord-269720-o81j3d1j 138 32 ) ) -RRB- cord-269720-o81j3d1j 138 33 , , , cord-269720-o81j3d1j 138 34 with with IN cord-269720-o81j3d1j 138 35 feline feline JJ cord-269720-o81j3d1j 138 36 infectious infectious JJ cord-269720-o81j3d1j 138 37 peritonitis peritonitis NN cord-269720-o81j3d1j 138 38 virus virus NN cord-269720-o81j3d1j 138 39 ( ( -LRB- cord-269720-o81j3d1j 138 40 FIPV FIPV NNP cord-269720-o81j3d1j 138 41 ) ) -RRB- cord-269720-o81j3d1j 138 42 and and CC cord-269720-o81j3d1j 138 43 canine canine JJ cord-269720-o81j3d1j 138 44 coronavirus coronavirus NN cord-269720-o81j3d1j 138 45 ( ( -LRB- cord-269720-o81j3d1j 138 46 CCV CCV NNP cord-269720-o81j3d1j 138 47 ) ) -RRB- cord-269720-o81j3d1j 138 48 , , , cord-269720-o81j3d1j 138 49 which which WDT cord-269720-o81j3d1j 138 50 are be VBP cord-269720-o81j3d1j 138 51 serologically serologically RB cord-269720-o81j3d1j 138 52 related relate VBN cord-269720-o81j3d1j 138 53 to to IN cord-269720-o81j3d1j 138 54 TGEV TGEV NNP cord-269720-o81j3d1j 138 55 , , , cord-269720-o81j3d1j 138 56 once once IN cord-269720-o81j3d1j 138 57 their -PRON- PRP$ cord-269720-o81j3d1j 138 58 sequences sequence NNS cord-269720-o81j3d1j 138 59 have have VBP cord-269720-o81j3d1j 138 60 been be VBN cord-269720-o81j3d1j 138 61 determined determine VBN cord-269720-o81j3d1j 138 62 . . . cord-269720-o81j3d1j 139 1 The the DT cord-269720-o81j3d1j 139 2 large large JJ cord-269720-o81j3d1j 139 3 variation variation NN cord-269720-o81j3d1j 139 4 in in IN cord-269720-o81j3d1j 139 5 sequence sequence NN cord-269720-o81j3d1j 139 6 length length NN cord-269720-o81j3d1j 139 7 and and CC cord-269720-o81j3d1j 139 8 content content NN cord-269720-o81j3d1j 139 9 made make VBD cord-269720-o81j3d1j 139 10 the the DT cord-269720-o81j3d1j 139 11 alignment alignment NN cord-269720-o81j3d1j 139 12 of of IN cord-269720-o81j3d1j 139 13 the the DT cord-269720-o81j3d1j 139 14 different different JJ cord-269720-o81j3d1j 139 15 leader leader NN cord-269720-o81j3d1j 139 16 sequences sequence NNS cord-269720-o81j3d1j 139 17 difficult difficult JJ cord-269720-o81j3d1j 139 18 . . . cord-269720-o81j3d1j 140 1 However however RB cord-269720-o81j3d1j 140 2 , , , cord-269720-o81j3d1j 140 3 alignment alignment NN cord-269720-o81j3d1j 140 4 of of IN cord-269720-o81j3d1j 140 5 the the DT cord-269720-o81j3d1j 140 6 six six CD cord-269720-o81j3d1j 140 7 different different JJ cord-269720-o81j3d1j 140 8 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 140 9 revealed reveal VBD cord-269720-o81j3d1j 140 10 that that IN cord-269720-o81j3d1j 140 11 they -PRON- PRP cord-269720-o81j3d1j 140 12 fell fall VBD cord-269720-o81j3d1j 140 13 into into IN cord-269720-o81j3d1j 140 14 two two CD cord-269720-o81j3d1j 140 15 groups group NNS cord-269720-o81j3d1j 140 16 . . . cord-269720-o81j3d1j 141 1 There there EX cord-269720-o81j3d1j 141 2 appears appear VBZ cord-269720-o81j3d1j 141 3 to to TO cord-269720-o81j3d1j 141 4 be be VB cord-269720-o81j3d1j 141 5 some some DT cord-269720-o81j3d1j 141 6 conservation conservation NN cord-269720-o81j3d1j 141 7 of of IN cord-269720-o81j3d1j 141 8 short short JJ cord-269720-o81j3d1j 141 9 sequence sequence NN cord-269720-o81j3d1j 141 10 motifs motif NNS cord-269720-o81j3d1j 141 11 between between IN cord-269720-o81j3d1j 141 12 the the DT cord-269720-o81j3d1j 141 13 seven seven CD cord-269720-o81j3d1j 141 14 leader leader NN cord-269720-o81j3d1j 141 15 sequences sequence NNS cord-269720-o81j3d1j 141 16 . . . cord-269720-o81j3d1j 142 1 Toward toward IN cord-269720-o81j3d1j 142 2 the the DT cord-269720-o81j3d1j 142 3 3 3 CD cord-269720-o81j3d1j 142 4 ' ' NN cord-269720-o81j3d1j 142 5 end end NN cord-269720-o81j3d1j 142 6 of of IN cord-269720-o81j3d1j 142 7 the the DT cord-269720-o81j3d1j 142 8 sequences sequence NNS cord-269720-o81j3d1j 142 9 , , , cord-269720-o81j3d1j 142 10 a a DT cord-269720-o81j3d1j 142 11 TAG tag NN cord-269720-o81j3d1j 142 12 motif motif NN cord-269720-o81j3d1j 142 13 is be VBZ cord-269720-o81j3d1j 142 14 conserved conserve VBN cord-269720-o81j3d1j 142 15 in in IN cord-269720-o81j3d1j 142 16 all all PDT cord-269720-o81j3d1j 142 17 the the DT cord-269720-o81j3d1j 142 18 leaders leader NNS cord-269720-o81j3d1j 142 19 , , , cord-269720-o81j3d1j 142 20 followed follow VBN cord-269720-o81j3d1j 142 21 by by IN cord-269720-o81j3d1j 142 22 a a DT cord-269720-o81j3d1j 142 23 string string NN cord-269720-o81j3d1j 142 24 of of IN cord-269720-o81j3d1j 142 25 Ts Ts NNP cord-269720-o81j3d1j 142 26 . . . cord-269720-o81j3d1j 143 1 In in IN cord-269720-o81j3d1j 143 2 five five CD cord-269720-o81j3d1j 143 3 out out IN cord-269720-o81j3d1j 143 4 of of IN cord-269720-o81j3d1j 143 5 seven seven CD cord-269720-o81j3d1j 143 6 of of IN cord-269720-o81j3d1j 143 7 the the DT cord-269720-o81j3d1j 143 8 sequences sequence NNS cord-269720-o81j3d1j 143 9 , , , cord-269720-o81j3d1j 143 10 this this DT cord-269720-o81j3d1j 143 11 motif motif NN cord-269720-o81j3d1j 143 12 is be VBZ cord-269720-o81j3d1j 143 13 TAGANNTT TAGANNTT NNP cord-269720-o81j3d1j 143 14 . . . cord-269720-o81j3d1j 144 1 About about RB cord-269720-o81j3d1j 144 2 ten ten CD cord-269720-o81j3d1j 144 3 nucleotides nucleotide NNS cord-269720-o81j3d1j 144 4 downstream downstream RB cord-269720-o81j3d1j 144 5 of of IN cord-269720-o81j3d1j 144 6 this this DT cord-269720-o81j3d1j 144 7 region region NN cord-269720-o81j3d1j 144 8 is be VBZ cord-269720-o81j3d1j 144 9 a a DT cord-269720-o81j3d1j 144 10 conserved conserve VBN cord-269720-o81j3d1j 144 11 CT CT NNP cord-269720-o81j3d1j 144 12 motif motif NN cord-269720-o81j3d1j 144 13 , , , cord-269720-o81j3d1j 144 14 which which WDT cord-269720-o81j3d1j 144 15 is be VBZ cord-269720-o81j3d1j 144 16 followed follow VBN cord-269720-o81j3d1j 144 17 by by IN cord-269720-o81j3d1j 144 18 a a DT cord-269720-o81j3d1j 144 19 series series NN cord-269720-o81j3d1j 144 20 of of IN cord-269720-o81j3d1j 144 21 nucleotides nucleotide NNS cord-269720-o81j3d1j 144 22 differing differ VBG cord-269720-o81j3d1j 144 23 in in IN cord-269720-o81j3d1j 144 24 number number NN cord-269720-o81j3d1j 144 25 , , , cord-269720-o81j3d1j 144 26 depending depend VBG cord-269720-o81j3d1j 144 27 on on IN cord-269720-o81j3d1j 144 28 the the DT cord-269720-o81j3d1j 144 29 coronavirus coronavirus NN cord-269720-o81j3d1j 144 30 , , , cord-269720-o81j3d1j 144 31 followed follow VBN cord-269720-o81j3d1j 144 32 by by IN cord-269720-o81j3d1j 144 33 the the DT cord-269720-o81j3d1j 144 34 postulated postulate VBN cord-269720-o81j3d1j 144 35 RNA RNA NNP cord-269720-o81j3d1j 144 36 polymerase polymerase NN cord-269720-o81j3d1j 144 37 - - HYPH cord-269720-o81j3d1j 144 38 leader leader NN cord-269720-o81j3d1j 144 39 complex complex JJ cord-269720-o81j3d1j 144 40 binding binding NN cord-269720-o81j3d1j 144 41 site site NN cord-269720-o81j3d1j 144 42 . . . cord-269720-o81j3d1j 145 1 The the DT cord-269720-o81j3d1j 145 2 largest large JJS cord-269720-o81j3d1j 145 3 number number NN cord-269720-o81j3d1j 145 4 of of IN cord-269720-o81j3d1j 145 5 nucleotides nucleotide NNS cord-269720-o81j3d1j 145 6 between between IN cord-269720-o81j3d1j 145 7 the the DT cord-269720-o81j3d1j 145 8 CT CT NNP cord-269720-o81j3d1j 145 9 motif motif NN cord-269720-o81j3d1j 145 10 and and CC cord-269720-o81j3d1j 145 11 the the DT cord-269720-o81j3d1j 145 12 consensus consensus NN cord-269720-o81j3d1j 145 13 sequence sequence NN cord-269720-o81j3d1j 145 14 are be VBP cord-269720-o81j3d1j 145 15 found find VBN cord-269720-o81j3d1j 145 16 on on IN cord-269720-o81j3d1j 145 17 TGEV TGEV NNP cord-269720-o81j3d1j 145 18 and and CC cord-269720-o81j3d1j 145 19 PRCV PRCV NNP cord-269720-o81j3d1j 145 20 , , , cord-269720-o81j3d1j 145 21 the the DT cord-269720-o81j3d1j 145 22 shortest short JJS cord-269720-o81j3d1j 145 23 is be VBZ cord-269720-o81j3d1j 145 24 found find VBN cord-269720-o81j3d1j 145 25 on on IN cord-269720-o81j3d1j 145 26 HCV HCV NNP cord-269720-o81j3d1j 145 27 ( ( -LRB- cord-269720-o81j3d1j 145 28 229E 229e CD cord-269720-o81j3d1j 145 29 ) ) -RRB- cord-269720-o81j3d1j 145 30 and and CC cord-269720-o81j3d1j 145 31 IBV IBV NNP cord-269720-o81j3d1j 145 32 . . . cord-269720-o81j3d1j 146 1 It -PRON- PRP cord-269720-o81j3d1j 146 2 is be VBZ cord-269720-o81j3d1j 146 3 interesting interesting JJ cord-269720-o81j3d1j 146 4 to to TO cord-269720-o81j3d1j 146 5 note note VB cord-269720-o81j3d1j 146 6 that that IN cord-269720-o81j3d1j 146 7 there there EX cord-269720-o81j3d1j 146 8 is be VBZ cord-269720-o81j3d1j 146 9 a a DT cord-269720-o81j3d1j 146 10 five five CD cord-269720-o81j3d1j 146 11 - - HYPH cord-269720-o81j3d1j 146 12 base base NN cord-269720-o81j3d1j 146 13 insert insert NN cord-269720-o81j3d1j 146 14 in in IN cord-269720-o81j3d1j 146 15 MHV MHV NNP cord-269720-o81j3d1j 146 16 strain strain NN cord-269720-o81j3d1j 146 17 JHM JHM NNP cord-269720-o81j3d1j 146 18 when when WRB cord-269720-o81j3d1j 146 19 compared compare VBN cord-269720-o81j3d1j 146 20 to to IN cord-269720-o81j3d1j 146 21 MHV MHV NNP cord-269720-o81j3d1j 146 22 strain strain NN cord-269720-o81j3d1j 146 23 A59 A59 NNP cord-269720-o81j3d1j 146 24 , , , cord-269720-o81j3d1j 146 25 which which WDT cord-269720-o81j3d1j 146 26 is be VBZ cord-269720-o81j3d1j 146 27 also also RB cord-269720-o81j3d1j 146 28 present present JJ cord-269720-o81j3d1j 146 29 in in IN cord-269720-o81j3d1j 146 30 HCV HCV NNP cord-269720-o81j3d1j 146 31 ( ( -LRB- cord-269720-o81j3d1j 146 32 OC43 OC43 NNP cord-269720-o81j3d1j 146 33 ) ) -RRB- cord-269720-o81j3d1j 146 34 within within IN cord-269720-o81j3d1j 146 35 this this DT cord-269720-o81j3d1j 146 36 region region NN cord-269720-o81j3d1j 146 37 . . . cord-269720-o81j3d1j 147 1 All all PDT cord-269720-o81j3d1j 147 2 the the DT cord-269720-o81j3d1j 147 3 mammalian mammalian JJ cord-269720-o81j3d1j 147 4 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 147 5 appear appear VBP cord-269720-o81j3d1j 147 6 to to TO cord-269720-o81j3d1j 147 7 have have VB cord-269720-o81j3d1j 147 8 the the DT cord-269720-o81j3d1j 147 9 motive motive NN cord-269720-o81j3d1j 147 10 CTAAAC ctaaac NN cord-269720-o81j3d1j 147 11 , , , cord-269720-o81j3d1j 147 12 except except IN cord-269720-o81j3d1j 147 13 HCV HCV NNP cord-269720-o81j3d1j 147 14 ( ( -LRB- cord-269720-o81j3d1j 147 15 OC43 OC43 NNP cord-269720-o81j3d1j 147 16 ) ) -RRB- cord-269720-o81j3d1j 147 17 , , , cord-269720-o81j3d1j 147 18 which which WDT cord-269720-o81j3d1j 147 19 has have VBZ cord-269720-o81j3d1j 147 20 CTAAAT CTAAAT NNP cord-269720-o81j3d1j 147 21 . . . cord-269720-o81j3d1j 148 1 Recent recent JJ cord-269720-o81j3d1j 148 2 sequence sequence NN cord-269720-o81j3d1j 148 3 data datum NNS cord-269720-o81j3d1j 148 4 suggest suggest VBP cord-269720-o81j3d1j 148 5 that that IN cord-269720-o81j3d1j 148 6 coronaviruses coronaviruse VBZ cord-269720-o81j3d1j 148 7 FIPV FIPV NNP cord-269720-o81j3d1j 148 8 and and CC cord-269720-o81j3d1j 148 9 BCV BCV NNP cord-269720-o81j3d1j 148 10 have have VBP cord-269720-o81j3d1j 148 11 ACTAAAC ACTAAAC NNP cord-269720-o81j3d1j 148 12 as as IN cord-269720-o81j3d1j 148 13 their -PRON- PRP$ cord-269720-o81j3d1j 148 14 mRNA mRNA NNP cord-269720-o81j3d1j 148 15 consensus consensus NN cord-269720-o81j3d1j 148 16 sequence sequence NN cord-269720-o81j3d1j 148 17 . . . cord-269720-o81j3d1j 149 1 Upstream upstream RB cord-269720-o81j3d1j 149 2 of of IN cord-269720-o81j3d1j 149 3 the the DT cord-269720-o81j3d1j 149 4 TAG tag NN cord-269720-o81j3d1j 149 5 motif motif NN cord-269720-o81j3d1j 149 6 there there EX cord-269720-o81j3d1j 149 7 is be VBZ cord-269720-o81j3d1j 149 8 an an DT cord-269720-o81j3d1j 149 9 ACT act NN cord-269720-o81j3d1j 149 10 motif motif NN cord-269720-o81j3d1j 149 11 occurring occur VBG cord-269720-o81j3d1j 149 12 in in IN cord-269720-o81j3d1j 149 13 six six CD cord-269720-o81j3d1j 149 14 out out IN cord-269720-o81j3d1j 149 15 of of IN cord-269720-o81j3d1j 149 16 seven seven CD cord-269720-o81j3d1j 149 17 sequences sequence NNS cord-269720-o81j3d1j 149 18 . . . cord-269720-o81j3d1j 150 1 Toward toward IN cord-269720-o81j3d1j 150 2 the the DT cord-269720-o81j3d1j 150 3 5 5 CD cord-269720-o81j3d1j 150 4 ' ' NN cord-269720-o81j3d1j 150 5 end end NN cord-269720-o81j3d1j 150 6 of of IN cord-269720-o81j3d1j 150 7 the the DT cord-269720-o81j3d1j 150 8 leader leader NN cord-269720-o81j3d1j 150 9 RNA RNA NNP cord-269720-o81j3d1j 150 10 sequences sequence NNS cord-269720-o81j3d1j 150 11 , , , cord-269720-o81j3d1j 150 12 the the DT cord-269720-o81j3d1j 150 13 homologies homology NNS cord-269720-o81j3d1j 150 14 are be VBP cord-269720-o81j3d1j 150 15 patchy patchy JJ cord-269720-o81j3d1j 150 16 and and CC cord-269720-o81j3d1j 150 17 limited limited JJ cord-269720-o81j3d1j 150 18 to to IN cord-269720-o81j3d1j 150 19 short short JJ cord-269720-o81j3d1j 150 20 matches match NNS cord-269720-o81j3d1j 150 21 , , , cord-269720-o81j3d1j 150 22 occurring occur VBG cord-269720-o81j3d1j 150 23 only only RB cord-269720-o81j3d1j 150 24 between between IN cord-269720-o81j3d1j 150 25 pairs pair NNS cord-269720-o81j3d1j 150 26 of of IN cord-269720-o81j3d1j 150 27 sequences sequence NNS cord-269720-o81j3d1j 150 28 . . . cord-269720-o81j3d1j 151 1 The the DT cord-269720-o81j3d1j 151 2 area area NN cord-269720-o81j3d1j 151 3 upstream upstream RB cord-269720-o81j3d1j 151 4 of of IN cord-269720-o81j3d1j 151 5 the the DT cord-269720-o81j3d1j 151 6 consensus consensus NN cord-269720-o81j3d1j 151 7 sequence sequence NN cord-269720-o81j3d1j 151 8 has have VBZ cord-269720-o81j3d1j 151 9 been be VBN cord-269720-o81j3d1j 151 10 suggested suggest VBN cord-269720-o81j3d1j 151 11 to to TO cord-269720-o81j3d1j 151 12 be be VB cord-269720-o81j3d1j 151 13 involved involve VBN cord-269720-o81j3d1j 151 14 in in IN cord-269720-o81j3d1j 151 15 the the DT cord-269720-o81j3d1j 151 16 binding binding NN cord-269720-o81j3d1j 151 17 of of IN cord-269720-o81j3d1j 151 18 nucleoprotein nucleoprotein NN cord-269720-o81j3d1j 151 19 to to IN cord-269720-o81j3d1j 151 20 the the DT cord-269720-o81j3d1j 151 21 leader leader NN cord-269720-o81j3d1j 151 22 RNA RNA NNP cord-269720-o81j3d1j 151 23 sequence sequence NN cord-269720-o81j3d1j 151 24 at at IN cord-269720-o81j3d1j 151 25 nucleotides nucleotide NNS cord-269720-o81j3d1j 151 26 56 56 CD cord-269720-o81j3d1j 151 27 - - SYM cord-269720-o81j3d1j 151 28 65 65 CD cord-269720-o81j3d1j 151 29 in in IN cord-269720-o81j3d1j 151 30 MHV MHV NNP cord-269720-o81j3d1j 151 31 ( ( -LRB- cord-269720-o81j3d1j 151 32 32 32 CD cord-269720-o81j3d1j 151 33 ) ) -RRB- cord-269720-o81j3d1j 151 34 . . . cord-269720-o81j3d1j 152 1 It -PRON- PRP cord-269720-o81j3d1j 152 2 was be VBD cord-269720-o81j3d1j 152 3 suggested suggest VBN cord-269720-o81j3d1j 152 4 that that IN cord-269720-o81j3d1j 152 5 mRNA mRNA NNP cord-269720-o81j3d1j 152 6 species specie NNS cord-269720-o81j3d1j 152 7 and and CC cord-269720-o81j3d1j 152 8 genomic genomic JJ cord-269720-o81j3d1j 152 9 RNA RNA NNP cord-269720-o81j3d1j 152 10 form form VBP cord-269720-o81j3d1j 152 11 a a DT cord-269720-o81j3d1j 152 12 complex complex NN cord-269720-o81j3d1j 152 13 with with IN cord-269720-o81j3d1j 152 14 the the DT cord-269720-o81j3d1j 152 15 nucleoprotein nucleoprotein NNP cord-269720-o81j3d1j 152 16 by by IN cord-269720-o81j3d1j 152 17 the the DT cord-269720-o81j3d1j 152 18 protein protein NN cord-269720-o81j3d1j 152 19 binding bind VBG cord-269720-o81j3d1j 152 20 to to IN cord-269720-o81j3d1j 152 21 or or CC cord-269720-o81j3d1j 152 22 near near IN cord-269720-o81j3d1j 152 23 the the DT cord-269720-o81j3d1j 152 24 leader leader NN cord-269720-o81j3d1j 152 25 sequence sequence NN cord-269720-o81j3d1j 152 26 attached attach VBN cord-269720-o81j3d1j 152 27 to to IN cord-269720-o81j3d1j 152 28 the the DT cord-269720-o81j3d1j 152 29 RNA RNA NNP cord-269720-o81j3d1j 152 30 molecules molecule NNS cord-269720-o81j3d1j 152 31 ( ( -LRB- cord-269720-o81j3d1j 152 32 33 33 CD cord-269720-o81j3d1j 152 33 ) ) -RRB- cord-269720-o81j3d1j 152 34 . . . cord-269720-o81j3d1j 153 1 Secondary secondary JJ cord-269720-o81j3d1j 153 2 structure structure NN cord-269720-o81j3d1j 153 3 analysis analysis NN cord-269720-o81j3d1j 153 4 of of IN cord-269720-o81j3d1j 153 5 the the DT cord-269720-o81j3d1j 153 6 leader leader NN cord-269720-o81j3d1j 153 7 RNA RNA NNP cord-269720-o81j3d1j 153 8 sequences sequence NNS cord-269720-o81j3d1j 153 9 showed show VBD cord-269720-o81j3d1j 153 10 that that IN cord-269720-o81j3d1j 153 11 all all PDT cord-269720-o81j3d1j 153 12 the the DT cord-269720-o81j3d1j 153 13 sequences sequence NNS cord-269720-o81j3d1j 153 14 except except IN cord-269720-o81j3d1j 153 15 for for IN cord-269720-o81j3d1j 153 16 IBV IBV NNP cord-269720-o81j3d1j 153 17 possess possess VBP cord-269720-o81j3d1j 153 18 a a DT cord-269720-o81j3d1j 153 19 putative putative JJ cord-269720-o81j3d1j 153 20 double double JJ cord-269720-o81j3d1j 153 21 stem stem NN cord-269720-o81j3d1j 153 22 - - HYPH cord-269720-o81j3d1j 153 23 loop loop NN cord-269720-o81j3d1j 153 24 structure structure NN cord-269720-o81j3d1j 153 25 ( ( -LRB- cord-269720-o81j3d1j 153 26 Fig Fig NNP cord-269720-o81j3d1j 153 27 . . . cord-269720-o81j3d1j 153 28 6 6 CD cord-269720-o81j3d1j 153 29 ) ) -RRB- cord-269720-o81j3d1j 153 30 . . . cord-269720-o81j3d1j 154 1 In in IN cord-269720-o81j3d1j 154 2 the the DT cord-269720-o81j3d1j 154 3 case case NN cord-269720-o81j3d1j 154 4 of of IN cord-269720-o81j3d1j 154 5 the the DT cord-269720-o81j3d1j 154 6 mammalian mammalian JJ cord-269720-o81j3d1j 154 7 coronaviruses coronaviruse NNS cord-269720-o81j3d1j 154 8 , , , cord-269720-o81j3d1j 154 9 the the DT cord-269720-o81j3d1j 154 10 consensus consensus NN cord-269720-o81j3d1j 154 11 sequences sequence NNS cord-269720-o81j3d1j 154 12 and and CC cord-269720-o81j3d1j 154 13 upstream upstream JJ cord-269720-o81j3d1j 154 14 regions region NNS cord-269720-o81j3d1j 154 15 of of IN cord-269720-o81j3d1j 154 16 homology homology NN cord-269720-o81j3d1j 154 17 are be VBP cord-269720-o81j3d1j 154 18 on on IN cord-269720-o81j3d1j 154 19 the the DT cord-269720-o81j3d1j 154 20 second second JJ cord-269720-o81j3d1j 154 21 stem stem NN cord-269720-o81j3d1j 154 22 - - HYPH cord-269720-o81j3d1j 154 23 loop loop NN cord-269720-o81j3d1j 154 24 structure structure NN cord-269720-o81j3d1j 154 25 , , , cord-269720-o81j3d1j 154 26 leaving leave VBG cord-269720-o81j3d1j 154 27 the the DT cord-269720-o81j3d1j 154 28 possibility possibility NN cord-269720-o81j3d1j 154 29 that that IN cord-269720-o81j3d1j 154 30 the the DT cord-269720-o81j3d1j 154 31 RNA RNA NNP cord-269720-o81j3d1j 154 32 - - HYPH cord-269720-o81j3d1j 154 33 dependent dependent JJ cord-269720-o81j3d1j 154 34 RNA RNA NNP cord-269720-o81j3d1j 154 35 polymerase polymerase NN cord-269720-o81j3d1j 154 36 could could MD cord-269720-o81j3d1j 154 37 interact interact VB cord-269720-o81j3d1j 154 38 with with IN cord-269720-o81j3d1j 154 39 the the DT cord-269720-o81j3d1j 154 40 first first JJ cord-269720-o81j3d1j 154 41 stem stem NN cord-269720-o81j3d1j 154 42 - - HYPH cord-269720-o81j3d1j 154 43 loop loop NN cord-269720-o81j3d1j 154 44 structure structure NN cord-269720-o81j3d1j 154 45 . . . cord-269720-o81j3d1j 155 1 The the DT cord-269720-o81j3d1j 155 2 IBV IBV NNP cord-269720-o81j3d1j 155 3 consensus consensus NN cord-269720-o81j3d1j 155 4 sequence sequence NN cord-269720-o81j3d1j 155 5 is be VBZ cord-269720-o81j3d1j 155 6 present present JJ cord-269720-o81j3d1j 155 7 on on IN cord-269720-o81j3d1j 155 8 the the DT cord-269720-o81j3d1j 155 9 free free JJ cord-269720-o81j3d1j 155 10 3 3 CD cord-269720-o81j3d1j 155 11 ' ' NN cord-269720-o81j3d1j 155 12 end end NN cord-269720-o81j3d1j 155 13 of of IN cord-269720-o81j3d1j 155 14 the the DT cord-269720-o81j3d1j 155 15 single single JJ cord-269720-o81j3d1j 155 16 stem stem NN cord-269720-o81j3d1j 155 17 - - HYPH cord-269720-o81j3d1j 155 18 loop loop NN cord-269720-o81j3d1j 155 19 structure structure NN cord-269720-o81j3d1j 155 20 , , , cord-269720-o81j3d1j 155 21 possibly possibly RB cord-269720-o81j3d1j 155 22 leaving leave VBG cord-269720-o81j3d1j 155 23 the the DT cord-269720-o81j3d1j 155 24 single single JJ cord-269720-o81j3d1j 155 25 stem stem NN cord-269720-o81j3d1j 155 26 - - HYPH cord-269720-o81j3d1j 155 27 loop loop NN cord-269720-o81j3d1j 155 28 structure structure NN cord-269720-o81j3d1j 155 29 to to TO cord-269720-o81j3d1j 155 30 interact interact VB cord-269720-o81j3d1j 155 31 with with IN cord-269720-o81j3d1j 155 32 the the DT cord-269720-o81j3d1j 155 33 polymerase polymerase NN cord-269720-o81j3d1j 155 34 . . . cord-269720-o81j3d1j 156 1 Virus virus NN cord-269720-o81j3d1j 156 2 Infections infection NNS cord-269720-o81j3d1j 156 3 of of IN cord-269720-o81j3d1j 156 4 Vertebrates Vertebrates NNPS cord-269720-o81j3d1j 156 5 ( ( -LRB- cord-269720-o81j3d1j 156 6 eds eds NNP cord-269720-o81j3d1j 156 7 ) ) -RRB- cord-269720-o81j3d1j 156 8 Coronaviruses Coronaviruses NNP cord-269720-o81j3d1j 156 9 Molecular Molecular NNP cord-269720-o81j3d1j 156 10 Cloning cloning NN cord-269720-o81j3d1j 156 11 : : : cord-269720-o81j3d1j 157 1 A a DT cord-269720-o81j3d1j 157 2 Laboratory Laboratory NNP cord-269720-o81j3d1j 157 3 Manual Manual NNP cord-269720-o81j3d1j 158 1 We -PRON- PRP cord-269720-o81j3d1j 158 2 thank thank VBP cord-269720-o81j3d1j 158 3 Miss Miss NNP cord-269720-o81j3d1j 158 4 K. K. NNP cord-269720-o81j3d1j 158 5 Mawditt Mawditt NNP cord-269720-o81j3d1j 158 6 , , , cord-269720-o81j3d1j 158 7 of of IN cord-269720-o81j3d1j 158 8 this this DT cord-269720-o81j3d1j 158 9 laboratory laboratory NN cord-269720-o81j3d1j 158 10 , , , cord-269720-o81j3d1j 158 11 for for IN cord-269720-o81j3d1j 158 12 synthesizing synthesize VBG cord-269720-o81j3d1j 158 13 oligos oligo NNS cord-269720-o81j3d1j 158 14 38 38 CD cord-269720-o81j3d1j 158 15 and and CC cord-269720-o81j3d1j 158 16 58 58 CD cord-269720-o81j3d1j 158 17 and and CC cord-269720-o81j3d1j 158 18 Dr. Dr. NNP cord-269720-o81j3d1j 158 19 S. S. NNP cord-269720-o81j3d1j 158 20 F. F. NNP cord-269720-o81j3d1j 158 21 Cartwright Cartwright NNP cord-269720-o81j3d1j 158 22 , , , cord-269720-o81j3d1j 158 23 Central Central NNP cord-269720-o81j3d1j 158 24 Veterinary Veterinary NNP cord-269720-o81j3d1j 158 25 Laboratory Laboratory NNP cord-269720-o81j3d1j 158 26 , , , cord-269720-o81j3d1j 158 27 Weybridge Weybridge NNP cord-269720-o81j3d1j 158 28 for for IN cord-269720-o81j3d1j 158 29 PRCV PRCV NNP cord-269720-o81j3d1j 158 30 strains strain VBZ cord-269720-o81j3d1j 158 31 86/137004 86/137004 CD cord-269720-o81j3d1j 158 32 and and CC cord-269720-o81j3d1j 158 33 86/135308 86/135308 CD cord-269720-o81j3d1j 158 34 . . . cord-269720-o81j3d1j 159 1 This this DT cord-269720-o81j3d1j 159 2 work work NN cord-269720-o81j3d1j 159 3 was be VBD cord-269720-o81j3d1j 159 4 supported support VBN cord-269720-o81j3d1j 159 5 by by IN cord-269720-o81j3d1j 159 6 a a DT cord-269720-o81j3d1j 159 7 research research NN cord-269720-o81j3d1j 159 8 contract contract NN cord-269720-o81j3d1j 159 9 from from IN cord-269720-o81j3d1j 159 10 the the DT cord-269720-o81j3d1j 159 11 Biomolecular Biomolecular NNP cord-269720-o81j3d1j 159 12 Engineering Engineering NNP cord-269720-o81j3d1j 159 13 Programme Programme NNP cord-269720-o81j3d1j 159 14 of of IN cord-269720-o81j3d1j 159 15 the the DT cord-269720-o81j3d1j 159 16 Commission Commission NNP cord-269720-o81j3d1j 159 17 of of IN cord-269720-o81j3d1j 159 18 the the DT cord-269720-o81j3d1j 159 19 European European NNP cord-269720-o81j3d1j 159 20 Communities Communities NNPS cord-269720-o81j3d1j 159 21 , , , cord-269720-o81j3d1j 159 22 contract contract NN cord-269720-o81j3d1j 160 1 No no UH cord-269720-o81j3d1j 160 2 . . . cord-269720-o81j3d1j 160 3 BAP-0235-UK(HI BAP-0235-UK(HI NNP cord-269720-o81j3d1j 160 4 ) ) -RRB- cord-269720-o81j3d1j 160 5 . . .