id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257715-pbcr81qm	Pignatelli, J.	Molecular characterization of a new PToV strain. Evolutionary implications	2009-03-20		.txt	text/plain	8278	389	53	Thus, there have been a few reports where indirect ELISA using partially purified BToV or BEV particles were used for torovirus serodiagnosis, but purification procedures are not affordable by all laboratories and, in addition, this assay would also provide low sensitivity for detection of antibodies to human and porcine toroviruses (Brown et al., 1987) . Lack of cross-reactivity between PToV and antibodies to other related viruses infecting pigs was confirmed by ELISA, Western blot and virus neutralization tests using ␣PRRSV, ␣PRCV, and ␣BRES serum samples, purified PToV N protein or BEV particles as toroviral antigens, and purified PRRSV and TGEV virions as related viruses (data not shown). In order to evaluate the potential use of the PToV-N protein as antigen for diagnostic ELISA to detect antibodies against porcine torovirus, 45 serum samples were collected from three Spanish farms located in Galicia, Navarra and Aragon and were analyzed by ELISA, virus neutralization assay and/or Western blot. Recombinant PToV-BRES-N protein was used to develop an ELISA assay to detect antibodies against torovirus in swine serum samples.	./cache/cord-257715-pbcr81qm.txt	./txt/cord-257715-pbcr81qm.txt