id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260835-ck9z5xsd	Kamau, Anthony Ndirangu	Porcine amino peptidase N domain VII has critical role in binding and entry of porcine epidemic diarrhea virus	2017-01-02		.txt	text/plain	4509	293	60	Results showed PEDV infection was restricted to pAPN and pAPN domain VII expressing NIH3T3 cells. Also, PEDV harvested from pAPN or domain VII expressing NIH3T3 cells was induced indirect plaques in Vero cells confirming successful entry and replication. In the current study, by constitutive expression of pAPN in non-susceptible NIH3T3 cells, we have sought to annotate the previous findings stipulating pAPN receptor function for PEDV. Susceptibility assays showed infection in pAPN domain-VII expressing cells but virus entry was abrogated in absence of seventh domain (pAPN DI-VI ) as shown in (Fig. 5A) . PEDV was also reported to interact with pAPN in porcine enterocytes (Li et al., 2009) , and is capable of infecting MDCK and ST cells expressing the pAPN receptor (Oh et al., 2003; Nam and Lee, 2010) . In our study, to determine the specific interactive point of pAPN wt with PEDV, domain mutants were expressed stably in NIH3T3 cells.	./cache/cord-260835-ck9z5xsd.txt	./txt/cord-260835-ck9z5xsd.txt