id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263439-oquk4t96	Park, Jung-Eun	Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells	2014-10-13		.txt	text/plain	5405	292	48	Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. Taken together, our findings reveal that PEDV enters Vero cells via clathrin-mediated endocytosis and requires serine proteolysis during infection. Based on these observations, we concluded that an exogenous protease, like trypsin, was necessary to induce cell-cell fusion in PEDV-infected Vero cells but not essentially required for virus-cell entry. So, we hypothesized that PEDV entry into Vero cells under the trypsin-free condition most likely occurred inside endosomal compartments where cellular proteases might operate similar to trypsin, facilitating S-mediated fusion of PEDV with the endosomal membrane. The infection inhibition assay using various substrates that interfere with endocytosis or lysosomotropic agents revealed that PEDV enters Vero cells via clathrin-mediated endocytic uptake and delivery of virus to an acidic intracellular compartment.	./cache/cord-263439-oquk4t96.txt	./txt/cord-263439-oquk4t96.txt