id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266453-v1hbust8	Sztuba-Solińska, Joanna	Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus	2012-10-16		.txt	text/plain	7845	370	52	We have previously described the efficient homologous recombination system between 5′ subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). A structured region near the 3 sgRNA3a polyA tail, referred to as the intergenic Bbox motif, participates in the assembly of the BMV replicase complex on RNA3 via interactions with protein 1a (Baumstrak and Ahlquist, 2001) , and it binds CP molecules via a specific peptide domains, as mapped by Yi et al. In a separate experiment, the Bbox-RNA3 was co-transfected with SG construct to determine whether the presence of the wt Bbox motif in sgRNA3a could rescue the previously reported high recombination frequency for unmutated RNAs. Indeed, among 100 cDNA clones, there were 42 recombinants (Fig. 2B) , of which the majority carried all three marker restriction sites (74%, 32 clones), while few had either single (BamHI -four clones) or double (BamHI/HindIII -three clones, BamHI/PstI -one clone, HindIII/PstI -two clones) restriction sites.	./cache/cord-266453-v1hbust8.txt	./txt/cord-266453-v1hbust8.txt