id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272383-pzivb0ro	Reddy, P.Seshidhar	Optimization of bovine coronavirus hemagglutinin-estrase glycoprotein expression in E3 deleted bovine adenovirus-3	2000-11-08		.txt	text/plain	4184	229	54	In an effort to optimize the expression, we have constructed several BAV-3 recombinants carrying the hemagglutinin esterase (HE) gene of bovine coronavirus (BCV) in the E3 region with or without exogenous transcription control elements. During infection of bovine cell lines, such recombinant BAVs produced large amounts of glycoprotein gD (a DNA virus gene), which has been shown to undergo proper post-translational modifications . This new transfer vector has two unique restriction enzyme sites (SrfI and SalI) for cloning of foreign genes and an overlap of 1992 bp on the left side and 3866 bp on the right side of the E3 region for efficient homologous recombination with plasmid pFBAV-302 , which dramatically increased the frequency of recombination in BJ 5183 cells. Our initial attempts to insert the BCV HE gene in the E3 region of plasmid pFBAV302 (E3 deleted full length BAV-3 genomic clone; Zakhartchouk et al., 1998) by homologous recombination in E.	./cache/cord-272383-pzivb0ro.txt	./txt/cord-272383-pzivb0ro.txt