id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272408-8flsy5o1	Chen, Rui	Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus	2020-01-15		.txt	text/plain	6054	363	55	The neutralizing activity of the rabbit and mouse anti -NTD, -CTD, and -S2 polyclonal antisera was assessed 4 weeks after the initial inoculation by a FFN assay, as previously described (Okda et al., 2015) . Sera from rabbits inoculated with NTD, CTD, and S2 were tested for neutralizing antibodies against PDCoV by ELISA, virus neutralization (VN), and fluorescent focus neutralization (FFN) assays. These results demonstrate that the NTD, CTD, and S2 proteins induced potent anti-PDCoV neutralizing antibody responses in the immunized animals. We found that three regions in PDCOV S protein, including NTD (aa 50-286), CTD (aa 278-616), and S2 (aa 601-1087), can induce neutralizing antibody responses, and the specific polyclonal mouse and rabbit sera efficiently inhibit PDCoV entry and infection in ST cells. Cell attachment domains of the porcine epidemic diarrhea virus spike protein are key targets of neutralizing antibodies	./cache/cord-272408-8flsy5o1.txt	./txt/cord-272408-8flsy5o1.txt