id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-280643-n8qjorqk	Wu, Kai-Lang	Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment	2005-04-26		.txt	text/plain	3749	252	60	To circumvent the problem that mutation in HBV genome may result in resistance when siRNA is further developed as an anti-viral drug, in this study, we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules that can specifically target two genes. To test the effectiveness of this system, we applied this new approach to express simultaneously two different 21-bp hairpin siRNA duplexes that specifically attack the HBs and HBx genes of HBV, respectively, in Bel-7402 and HepG2.2.15 cells. Results indicated that dual siRNA could simultaneously inhibit the expression of HBs and HBx gene by 83.7% and 87.5%, respectively, based on luciferase assays. Results indicated that the levels of HBV core associ-ated DNA were significantly decreased in the cells transfected by HBSXsiRNA, HBS 1 siRNA, HBS 2 siRNA, and HBX 2 siRNA with reduction rate of 90.2%, 85.7 %, 81.3%, and 60.4%, respectively, compared with that of vector control (Fig. 5a) .	./cache/cord-280643-n8qjorqk.txt	./txt/cord-280643-n8qjorqk.txt