id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309205-l8vjtrjq	Shirato, Kazuya	Differential susceptibility of macrophages to serotype II feline coronaviruses correlates with differences in the viral spike protein	2018-08-15		.txt	text/plain	6689	327	50	The ability to infect and replicate in monocytes/macrophages is a critically distinguishing feature between the two feline coronavirus (FCoV) pathotypes: feline enteric coronavirus (FECV; low-virulent) and feline infectious peritonitis virus (FIPV; lethal). Previously, by comparing serotype II strains FIPV 79-1146 and FECV 79-1683 and recombinant chimeric forms thereof in cultured feline bone marrow macrophages, we mapped this difference to the C-terminal part of the viral spike (S) protein (S2). Despite some concerns relating to the precise identity of the FCoV strains 79-1683 and 79-1146, to be discussed later, but lacking better options to address this critical issue in the pathogenesis of FIP, we continued in the present study with investigating the contributions of the amino acids in the spike S2 domain differing between the prototypic strains to the distinguishing macrophage tropism of these viruses. There are eleven amino acid differences in the C-terminal domain of the S proteins of FIPV 79-1146 and FECV 79-1683 to which we mapped these viruses' differential ability to infect macrophages (Fig. 1c) .	./cache/cord-309205-l8vjtrjq.txt	./txt/cord-309205-l8vjtrjq.txt