id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312489-ywep0c08	Andoh, Kiyohiko	Decreased neutralizing antigenicity in IBV S1 protein expressed from mammalian cells	2015-10-02		.txt	text/plain	5039	246	43	We evaluated the antigenicity of recombinant infectious bronchitis virus (IBV) S1 protein expressed in mammalian cells. Native S1 ELISA detected similar titers in the sera of animals immunized with recombinant S1 protein compared to those in sera of chickens immunized with inactivated virus (Fig. 6b ) (Control experiments (data not shown) demonstrated that mock and unvaccinated control groups did not differ from each other in VN and ELISA tests of anti-S1 activity.) These results indicated that while recombinant S1 protein retained antigenicity (the ability to induce antibodies against S1 protein), the resulting antibodies was decreased its neutralizing activity, in contrast to those induced by inactivated virus. The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens Induction of protective immunity in chickens vaccinated with infectious bronchitis virus S1 glycoprotein expressed by a recombinant baculovirus	./cache/cord-312489-ywep0c08.txt	./txt/cord-312489-ywep0c08.txt