id author title date pages extension mime words sentences flesch summary cache txt cord-346104-18x8u2oe Black, Wendy Identification of gammaherpesvirus infection in free-ranging black bears (Ursus americanus) 2019-01-02 .txt text/plain 5163 298 56 Herpesvirus infection in the bear tissues and tissue cultures were detected by PCR using degenerate primers specific to the DNA polymerase gene (DPOL) and glycoprotein B gene (gB). To determine whether the observed viral particles are herpesviruses, tissues collected from the two NV black bears B1 and B2 were examined by nested PCR using panherpesvirus primers (Pozo et al., 2016 ) and herpesvirus consensus primers (VanDevanter et al., 1996) . To determine how common this UrHV-1 like virus is in black bear population, various tissues from 15 bears collected from Nevada, California, and Oregon were analyzed by this hybrid nested PCR with panherpesvirus primers in primary reaction and UrHV-1specific primers in the secondary reactions. Positive amplification with UrHV-1 specific primers was observed in total DNA of white blood cells (WBC) from two black bears from Nevada and one from Oregon. ./cache/cord-346104-18x8u2oe.txt ./txt/cord-346104-18x8u2oe.txt