id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-347924-w06ho8sn	Wen, Jin-Sheng	Computational prediction and identification of dengue virus-specific CD4(+) T-cell epitopes	2007-12-03		.txt	text/plain	3323	181	57	In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-γ. Currently, many studies suggested that dengue-specific T-cell immune response are hypothesized to play an important role in the immunopathogenesis of DHF during a secondary DEN virus infection, and induction of immunopathology by T lymphocytes may occur by various mechanisms, including cell-mediated cytotoxicity and/or cytokine production (Miskovsky et al., 1994; Vergelli et al., 1997) . In the present study, a combination of bioinformatics tools (epitope-prediction programs RANKpep) and in vitro assays (enzyme-linked immunospot assay (ELISPOT) and intracel-lular cytokine staining assay (ICS)) was used to screen and select antigen sequences as potential DEN-specific CD4 + T-cell epitopes, then the selected sequences are tested for biological function by their activation of T cells of DEN infected populations.	./cache/cord-347924-w06ho8sn.txt	./txt/cord-347924-w06ho8sn.txt