id	sid	tid	token	lemma	pos
cord-275016-ij5yaqkx	1	1	key	key	JJ
cord-275016-ij5yaqkx	1	2	:	:	:
cord-275016-ij5yaqkx	1	3	cord-275016-ij5yaqkx	cord-275016-ij5yaqkx	CD
cord-275016-ij5yaqkx	1	4	authors	author	NNS
cord-275016-ij5yaqkx	1	5	:	:	:
cord-275016-ij5yaqkx	1	6	Someya	Someya	NNP
cord-275016-ij5yaqkx	1	7	,	,	,
cord-275016-ij5yaqkx	1	8	Yuichi	Yuichi	NNP
cord-275016-ij5yaqkx	1	9	;	;	:
cord-275016-ij5yaqkx	1	10	Takeda	Takeda	NNP
cord-275016-ij5yaqkx	1	11	,	,	,
cord-275016-ij5yaqkx	1	12	Naokazu	Naokazu	NNPS
cord-275016-ij5yaqkx	1	13	;	;	:
cord-275016-ij5yaqkx	1	14	Miyamura	Miyamura	NNP
cord-275016-ij5yaqkx	1	15	,	,	,
cord-275016-ij5yaqkx	1	16	Tatsuo	Tatsuo	NNP
cord-275016-ij5yaqkx	1	17	title	title	NN
cord-275016-ij5yaqkx	1	18	:	:	:
cord-275016-ij5yaqkx	1	19	Characterization	characterization	NN
cord-275016-ij5yaqkx	1	20	of	of	IN
cord-275016-ij5yaqkx	1	21	the	the	DT
cord-275016-ij5yaqkx	1	22	norovirus	norovirus	NN
cord-275016-ij5yaqkx	1	23	3C	3c	NN
cord-275016-ij5yaqkx	1	24	-	-	HYPH
cord-275016-ij5yaqkx	1	25	like	like	JJ
cord-275016-ij5yaqkx	1	26	protease	protease	NN
cord-275016-ij5yaqkx	1	27	date	date	NN
cord-275016-ij5yaqkx	1	28	:	:	:
cord-275016-ij5yaqkx	1	29	2005	2005	CD
cord-275016-ij5yaqkx	1	30	-	-	HYPH
cord-275016-ij5yaqkx	1	31	03	03	CD
cord-275016-ij5yaqkx	1	32	-	-	HYPH
cord-275016-ij5yaqkx	1	33	08	08	CD
cord-275016-ij5yaqkx	1	34	journal	journal	NN
cord-275016-ij5yaqkx	1	35	:	:	:
cord-275016-ij5yaqkx	2	1	Virus	virus	NN
cord-275016-ij5yaqkx	2	2	Res	res	NN
cord-275016-ij5yaqkx	2	3	DOI	doi	NN
cord-275016-ij5yaqkx	2	4	:	:	:
cord-275016-ij5yaqkx	3	1	10.1016	10.1016	CD
cord-275016-ij5yaqkx	3	2	/	/	SYM
cord-275016-ij5yaqkx	3	3	j.virusres.2005.02.002	j.virusres.2005.02.002	CD
cord-275016-ij5yaqkx	3	4	sha	sha	XX
cord-275016-ij5yaqkx	3	5	:	:	:
cord-275016-ij5yaqkx	4	1	f0283b5c2d9e5c88d7561a6d8d5332d5221dd7d0	f0283b5c2d9e5c88d7561a6d8d5332d5221dd7d0	NNP
cord-275016-ij5yaqkx	4	2	doc_id	doc_id	CD
cord-275016-ij5yaqkx	4	3	:	:	:
cord-275016-ij5yaqkx	4	4	275016	275016	CD
cord-275016-ij5yaqkx	4	5	cord_uid	cord_uid	NNS
cord-275016-ij5yaqkx	4	6	:	:	:
cord-275016-ij5yaqkx	4	7	ij5yaqkx	ij5yaqkx	.
cord-275016-ij5yaqkx	5	1	The	the	DT
cord-275016-ij5yaqkx	5	2	recombinant	recombinant	JJ
cord-275016-ij5yaqkx	5	3	3C	3c	NN
cord-275016-ij5yaqkx	5	4	-	-	HYPH
cord-275016-ij5yaqkx	5	5	like	like	JJ
cord-275016-ij5yaqkx	5	6	protease	protease	NN
cord-275016-ij5yaqkx	5	7	of	of	IN
cord-275016-ij5yaqkx	5	8	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	5	9	virus	virus	NN
cord-275016-ij5yaqkx	5	10	,	,	,
cord-275016-ij5yaqkx	5	11	a	a	DT
cord-275016-ij5yaqkx	5	12	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	5	13	,	,	,
cord-275016-ij5yaqkx	5	14	expressed	express	VBN
cord-275016-ij5yaqkx	5	15	in	in	IN
cord-275016-ij5yaqkx	5	16	Escherichia	Escherichia	NNP
cord-275016-ij5yaqkx	5	17	coli	coli	NNS
cord-275016-ij5yaqkx	5	18	cells	cell	NNS
cord-275016-ij5yaqkx	5	19	was	be	VBD
cord-275016-ij5yaqkx	5	20	purified	purify	VBN
cord-275016-ij5yaqkx	5	21	and	and	CC
cord-275016-ij5yaqkx	5	22	characterized	characterize	VBN
cord-275016-ij5yaqkx	5	23	as	as	IN
cord-275016-ij5yaqkx	5	24	to	to	IN
cord-275016-ij5yaqkx	5	25	effects	effect	NNS
cord-275016-ij5yaqkx	5	26	of	of	IN
cord-275016-ij5yaqkx	5	27	pH	ph	NN
cord-275016-ij5yaqkx	5	28	,	,	,
cord-275016-ij5yaqkx	5	29	temperature	temperature	NN
cord-275016-ij5yaqkx	5	30	,	,	,
cord-275016-ij5yaqkx	5	31	salt	salt	NN
cord-275016-ij5yaqkx	5	32	contents	content	NNS
cord-275016-ij5yaqkx	5	33	,	,	,
cord-275016-ij5yaqkx	5	34	and	and	CC
cord-275016-ij5yaqkx	5	35	SH	sh	NN
cord-275016-ij5yaqkx	5	36	reagents	reagent	NNS
cord-275016-ij5yaqkx	5	37	on	on	IN
cord-275016-ij5yaqkx	5	38	its	-PRON-	PRP$
cord-275016-ij5yaqkx	5	39	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	5	40	activity	activity	NN
cord-275016-ij5yaqkx	5	41	.	.	.
cord-275016-ij5yaqkx	6	1	The	the	DT
cord-275016-ij5yaqkx	6	2	optimal	optimal	JJ
cord-275016-ij5yaqkx	6	3	pH	ph	NN
cord-275016-ij5yaqkx	6	4	and	and	CC
cord-275016-ij5yaqkx	6	5	temperature	temperature	NN
cord-275016-ij5yaqkx	6	6	of	of	IN
cord-275016-ij5yaqkx	6	7	the	the	DT
cord-275016-ij5yaqkx	6	8	3C	3c	NN
cord-275016-ij5yaqkx	6	9	-	-	HYPH
cord-275016-ij5yaqkx	6	10	like	like	JJ
cord-275016-ij5yaqkx	6	11	protease	protease	NN
cord-275016-ij5yaqkx	6	12	for	for	IN
cord-275016-ij5yaqkx	6	13	the	the	DT
cord-275016-ij5yaqkx	6	14	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	6	15	activity	activity	NN
cord-275016-ij5yaqkx	6	16	were	be	VBD
cord-275016-ij5yaqkx	6	17	8.6	8.6	CD
cord-275016-ij5yaqkx	6	18	and	and	CC
cord-275016-ij5yaqkx	6	19	37	37	CD
cord-275016-ij5yaqkx	6	20	°	°	NN
cord-275016-ij5yaqkx	6	21	C	c	NN
cord-275016-ij5yaqkx	6	22	,	,	,
cord-275016-ij5yaqkx	6	23	respectively	respectively	RB
cord-275016-ij5yaqkx	6	24	.	.	.
cord-275016-ij5yaqkx	7	1	Increased	increase	VBN
cord-275016-ij5yaqkx	7	2	concentration	concentration	NN
cord-275016-ij5yaqkx	7	3	(	(	-LRB-
cord-275016-ij5yaqkx	7	4	∼100	∼100	NNP
cord-275016-ij5yaqkx	7	5	mM	mM	NNP
cord-275016-ij5yaqkx	7	6	)	)	-RRB-
cord-275016-ij5yaqkx	7	7	of	of	IN
cord-275016-ij5yaqkx	7	8	monovalent	monovalent	JJ
cord-275016-ij5yaqkx	7	9	cations	cation	NNS
cord-275016-ij5yaqkx	7	10	such	such	JJ
cord-275016-ij5yaqkx	7	11	as	as	IN
cord-275016-ij5yaqkx	7	12	Na(+	Na(+	NNP
cord-275016-ij5yaqkx	7	13	)	)	-RRB-
cord-275016-ij5yaqkx	7	14	and	and	CC
cord-275016-ij5yaqkx	7	15	K(+	k(+	RB
cord-275016-ij5yaqkx	7	16	)	)	-RRB-
cord-275016-ij5yaqkx	7	17	was	be	VBD
cord-275016-ij5yaqkx	7	18	inhibitory	inhibitory	JJ
cord-275016-ij5yaqkx	7	19	to	to	IN
cord-275016-ij5yaqkx	7	20	the	the	DT
cord-275016-ij5yaqkx	7	21	activity	activity	NN
cord-275016-ij5yaqkx	7	22	.	.	.
cord-275016-ij5yaqkx	8	1	Hg(2	Hg(2	NNP
cord-275016-ij5yaqkx	8	2	+	+	CC
cord-275016-ij5yaqkx	8	3	)	)	-RRB-
cord-275016-ij5yaqkx	8	4	and	and	CC
cord-275016-ij5yaqkx	8	5	Zn(2	Zn(2	NNP
cord-275016-ij5yaqkx	8	6	+	+	NN
cord-275016-ij5yaqkx	8	7	)	)	-RRB-
cord-275016-ij5yaqkx	8	8	remarkably	remarkably	RB
cord-275016-ij5yaqkx	8	9	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	8	10	the	the	DT
cord-275016-ij5yaqkx	8	11	protease	protease	NN
cord-275016-ij5yaqkx	8	12	activity	activity	NN
cord-275016-ij5yaqkx	8	13	,	,	,
cord-275016-ij5yaqkx	8	14	while	while	IN
cord-275016-ij5yaqkx	8	15	Mg(2	Mg(2	NNP
cord-275016-ij5yaqkx	8	16	+	+	SYM
cord-275016-ij5yaqkx	8	17	)	)	-RRB-
cord-275016-ij5yaqkx	8	18	and	and	CC
cord-275016-ij5yaqkx	8	19	Ca(2	Ca(2	NNP
cord-275016-ij5yaqkx	9	1	+	+	LS
cord-275016-ij5yaqkx	9	2	)	)	-RRB-
cord-275016-ij5yaqkx	9	3	had	have	VBD
cord-275016-ij5yaqkx	9	4	no	no	DT
cord-275016-ij5yaqkx	9	5	virtual	virtual	JJ
cord-275016-ij5yaqkx	9	6	effect	effect	NN
cord-275016-ij5yaqkx	9	7	.	.	.
cord-275016-ij5yaqkx	10	1	Several	several	JJ
cord-275016-ij5yaqkx	10	2	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	10	3	reagents	reagent	NNS
cord-275016-ij5yaqkx	10	4	such	such	JJ
cord-275016-ij5yaqkx	10	5	as	as	IN
cord-275016-ij5yaqkx	10	6	p	p	NN
cord-275016-ij5yaqkx	10	7	-	-	HYPH
cord-275016-ij5yaqkx	10	8	chloromercuribenzoic	chloromercuribenzoic	JJ
cord-275016-ij5yaqkx	10	9	acid	acid	NN
cord-275016-ij5yaqkx	10	10	,	,	,
cord-275016-ij5yaqkx	10	11	methyl	methyl	NN
cord-275016-ij5yaqkx	10	12	methanethiosulfonate	methanethiosulfonate	NN
cord-275016-ij5yaqkx	10	13	,	,	,
cord-275016-ij5yaqkx	10	14	N	N	NNP
cord-275016-ij5yaqkx	10	15	-	-	HYPH
cord-275016-ij5yaqkx	10	16	ethylmaleimide	ethylmaleimide	NNP
cord-275016-ij5yaqkx	10	17	and	and	CC
cord-275016-ij5yaqkx	10	18	N	N	NNP
cord-275016-ij5yaqkx	10	19	-	-	HYPH
cord-275016-ij5yaqkx	10	20	phenylmaleimide	phenylmaleimide	NNP
cord-275016-ij5yaqkx	10	21	also	also	RB
cord-275016-ij5yaqkx	10	22	blocked	block	VBD
cord-275016-ij5yaqkx	10	23	the	the	DT
cord-275016-ij5yaqkx	10	24	activity	activity	NN
cord-275016-ij5yaqkx	10	25	,	,	,
cord-275016-ij5yaqkx	10	26	confirming	confirm	VBG
cord-275016-ij5yaqkx	10	27	the	the	DT
cord-275016-ij5yaqkx	10	28	previous	previous	JJ
cord-275016-ij5yaqkx	10	29	result	result	NN
cord-275016-ij5yaqkx	10	30	that	that	IN
cord-275016-ij5yaqkx	10	31	cysteine	cysteine	NN
cord-275016-ij5yaqkx	10	32	residue(s	residue(s	NN
cord-275016-ij5yaqkx	10	33	)	)	-RRB-
cord-275016-ij5yaqkx	10	34	were	be	VBD
cord-275016-ij5yaqkx	10	35	responsible	responsible	JJ
cord-275016-ij5yaqkx	10	36	for	for	IN
cord-275016-ij5yaqkx	10	37	the	the	DT
cord-275016-ij5yaqkx	10	38	proteolysis	proteolysis	NN
cord-275016-ij5yaqkx	10	39	.	.	.
cord-275016-ij5yaqkx	11	1	Viral	viral	JJ
cord-275016-ij5yaqkx	11	2	proteases	protease	NNS
cord-275016-ij5yaqkx	11	3	play	play	VBP
cord-275016-ij5yaqkx	11	4	a	a	DT
cord-275016-ij5yaqkx	11	5	central	central	JJ
cord-275016-ij5yaqkx	11	6	role	role	NN
cord-275016-ij5yaqkx	11	7	in	in	IN
cord-275016-ij5yaqkx	11	8	the	the	DT
cord-275016-ij5yaqkx	11	9	maturation	maturation	NN
cord-275016-ij5yaqkx	11	10	of	of	IN
cord-275016-ij5yaqkx	11	11	functional	functional	JJ
cord-275016-ij5yaqkx	11	12	viral	viral	JJ
cord-275016-ij5yaqkx	11	13	proteins	protein	NNS
cord-275016-ij5yaqkx	11	14	,	,	,
cord-275016-ij5yaqkx	11	15	and	and	CC
cord-275016-ij5yaqkx	11	16	hence	hence	RB
cord-275016-ij5yaqkx	11	17	in	in	IN
cord-275016-ij5yaqkx	11	18	its	-PRON-	PRP$
cord-275016-ij5yaqkx	11	19	genome	genome	NN
cord-275016-ij5yaqkx	11	20	replication	replication	NN
cord-275016-ij5yaqkx	11	21	and	and	CC
cord-275016-ij5yaqkx	11	22	the	the	DT
cord-275016-ij5yaqkx	11	23	formation	formation	NN
cord-275016-ij5yaqkx	11	24	of	of	IN
cord-275016-ij5yaqkx	11	25	virus	virus	NN
cord-275016-ij5yaqkx	11	26	particles	particle	NNS
cord-275016-ij5yaqkx	11	27	(	(	-LRB-
cord-275016-ij5yaqkx	11	28	Dougherty	Dougherty	NNP
cord-275016-ij5yaqkx	11	29	and	and	CC
cord-275016-ij5yaqkx	11	30	Semler	Semler	NNP
cord-275016-ij5yaqkx	11	31	,	,	,
cord-275016-ij5yaqkx	11	32	1993	1993	CD
cord-275016-ij5yaqkx	11	33	)	)	-RRB-
cord-275016-ij5yaqkx	11	34	.	.	.
cord-275016-ij5yaqkx	12	1	The	the	DT
cord-275016-ij5yaqkx	12	2	3C	3c	NN
cord-275016-ij5yaqkx	12	3	and	and	CC
cord-275016-ij5yaqkx	12	4	3C	3c	NN
cord-275016-ij5yaqkx	12	5	-	-	HYPH
cord-275016-ij5yaqkx	12	6	like	like	JJ
cord-275016-ij5yaqkx	12	7	proteases	protease	NNS
cord-275016-ij5yaqkx	12	8	belonging	belong	VBG
cord-275016-ij5yaqkx	12	9	to	to	IN
cord-275016-ij5yaqkx	12	10	the	the	DT
cord-275016-ij5yaqkx	12	11	chymotrypsin	chymotrypsin	VB
cord-275016-ij5yaqkx	12	12	-	-	HYPH
cord-275016-ij5yaqkx	12	13	like	like	JJ
cord-275016-ij5yaqkx	12	14	protease	protease	NN
cord-275016-ij5yaqkx	12	15	superfamily	superfamily	NN
cord-275016-ij5yaqkx	12	16	are	be	VBP
cord-275016-ij5yaqkx	12	17	found	find	VBN
cord-275016-ij5yaqkx	12	18	in	in	IN
cord-275016-ij5yaqkx	12	19	several	several	JJ
cord-275016-ij5yaqkx	12	20	animal	animal	NN
cord-275016-ij5yaqkx	12	21	,	,	,
cord-275016-ij5yaqkx	12	22	insect	insect	NN
cord-275016-ij5yaqkx	12	23	,	,	,
cord-275016-ij5yaqkx	12	24	and	and	CC
cord-275016-ij5yaqkx	12	25	plant	plant	NN
cord-275016-ij5yaqkx	12	26	virus	virus	NN
cord-275016-ij5yaqkx	12	27	families	family	NNS
cord-275016-ij5yaqkx	12	28	such	such	JJ
cord-275016-ij5yaqkx	12	29	as	as	IN
cord-275016-ij5yaqkx	12	30	Picornaviridae	Picornaviridae	NNP
cord-275016-ij5yaqkx	12	31	,	,	,
cord-275016-ij5yaqkx	12	32	Comoviridae	Comoviridae	NNP
cord-275016-ij5yaqkx	12	33	,	,	,
cord-275016-ij5yaqkx	12	34	Caliciviridae	Caliciviridae	NNP
cord-275016-ij5yaqkx	12	35	,	,	,
cord-275016-ij5yaqkx	12	36	Coronaviridaee	Coronaviridaee	NNP
cord-275016-ij5yaqkx	12	37	,	,	,
cord-275016-ij5yaqkx	12	38	and	and	CC
cord-275016-ij5yaqkx	12	39	Arteriviridae	Arteriviridae	NNP
cord-275016-ij5yaqkx	12	40	(	(	-LRB-
cord-275016-ij5yaqkx	12	41	Dougherty	Dougherty	NNP
cord-275016-ij5yaqkx	12	42	and	and	CC
cord-275016-ij5yaqkx	12	43	Semler	Semler	NNP
cord-275016-ij5yaqkx	12	44	,	,	,
cord-275016-ij5yaqkx	12	45	1993	1993	CD
cord-275016-ij5yaqkx	12	46	;	;	:
cord-275016-ij5yaqkx	12	47	Gorbalenya	Gorbalenya	NNP
cord-275016-ij5yaqkx	12	48	et	et	NNP
cord-275016-ij5yaqkx	12	49	al	al	NNP
cord-275016-ij5yaqkx	12	50	.	.	NNP
cord-275016-ij5yaqkx	12	51	,	,	,
cord-275016-ij5yaqkx	12	52	1989	1989	CD
cord-275016-ij5yaqkx	12	53	)	)	-RRB-
cord-275016-ij5yaqkx	12	54	.	.	.
cord-275016-ij5yaqkx	13	1	Their	-PRON-	PRP$
cord-275016-ij5yaqkx	13	2	three	three	CD
cord-275016-ij5yaqkx	13	3	-	-	HYPH
cord-275016-ij5yaqkx	13	4	dimensional	dimensional	JJ
cord-275016-ij5yaqkx	13	5	structures	structure	NNS
cord-275016-ij5yaqkx	13	6	have	have	VBP
cord-275016-ij5yaqkx	13	7	been	be	VBN
cord-275016-ij5yaqkx	13	8	determined	determine	VBN
cord-275016-ij5yaqkx	13	9	by	by	IN
cord-275016-ij5yaqkx	13	10	X	x	NN
cord-275016-ij5yaqkx	13	11	-	-	NN
cord-275016-ij5yaqkx	13	12	ray	ray	JJ
cord-275016-ij5yaqkx	13	13	crystallography	crystallography	NN
cord-275016-ij5yaqkx	13	14	in	in	IN
cord-275016-ij5yaqkx	13	15	poliovirus	poliovirus	NN
cord-275016-ij5yaqkx	13	16	,	,	,
cord-275016-ij5yaqkx	13	17	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	13	18	(	(	-LRB-
cord-275016-ij5yaqkx	13	19	Matthews	Matthews	NNP
cord-275016-ij5yaqkx	13	20	et	et	NNP
cord-275016-ij5yaqkx	13	21	al	al	NNP
cord-275016-ij5yaqkx	13	22	.	.	NNP
cord-275016-ij5yaqkx	13	23	,	,	,
cord-275016-ij5yaqkx	13	24	1994	1994	CD
cord-275016-ij5yaqkx	13	25	)	)	-RRB-
cord-275016-ij5yaqkx	13	26	,	,	,
cord-275016-ij5yaqkx	13	27	hepatitis	hepatitis	NNP
cord-275016-ij5yaqkx	13	28	A	a	DT
cord-275016-ij5yaqkx	13	29	virus	virus	NN
cord-275016-ij5yaqkx	13	30	(	(	-LRB-
cord-275016-ij5yaqkx	13	31	Bergmann	Bergmann	NNP
cord-275016-ij5yaqkx	13	32	et	et	NNP
cord-275016-ij5yaqkx	13	33	al	al	NNP
cord-275016-ij5yaqkx	13	34	.	.	NNP
cord-275016-ij5yaqkx	13	35	,	,	,
cord-275016-ij5yaqkx	13	36	1997	1997	CD
cord-275016-ij5yaqkx	13	37	)	)	-RRB-
cord-275016-ij5yaqkx	13	38	,	,	,
cord-275016-ij5yaqkx	13	39	and	and	CC
cord-275016-ij5yaqkx	13	40	coronavirus	coronavirus	NN
cord-275016-ij5yaqkx	13	41	(	(	-LRB-
cord-275016-ij5yaqkx	13	42	Anand	Anand	NNP
cord-275016-ij5yaqkx	13	43	et	et	NNP
cord-275016-ij5yaqkx	13	44	al	al	NNP
cord-275016-ij5yaqkx	13	45	.	.	NNP
cord-275016-ij5yaqkx	13	46	,	,	,
cord-275016-ij5yaqkx	13	47	2002	2002	CD
cord-275016-ij5yaqkx	13	48	Yang	Yang	NNP
cord-275016-ij5yaqkx	13	49	et	et	NNP
cord-275016-ij5yaqkx	13	50	al	al	NNP
cord-275016-ij5yaqkx	13	51	.	.	NNP
cord-275016-ij5yaqkx	13	52	,	,	,
cord-275016-ij5yaqkx	13	53	2003	2003	CD
cord-275016-ij5yaqkx	13	54	)	)	-RRB-
cord-275016-ij5yaqkx	13	55	,	,	,
cord-275016-ij5yaqkx	13	56	tobacco	tobacco	NN
cord-275016-ij5yaqkx	13	57	etch	etch	NN
cord-275016-ij5yaqkx	13	58	virus	virus	NN
cord-275016-ij5yaqkx	13	59	(	(	-LRB-
cord-275016-ij5yaqkx	13	60	Phan	Phan	NNP
cord-275016-ij5yaqkx	13	61	et	et	NNP
cord-275016-ij5yaqkx	13	62	al	al	NNP
cord-275016-ij5yaqkx	13	63	.	.	NNP
cord-275016-ij5yaqkx	13	64	,	,	,
cord-275016-ij5yaqkx	13	65	2002	2002	CD
cord-275016-ij5yaqkx	13	66	)	)	-RRB-
cord-275016-ij5yaqkx	13	67	and	and	CC
cord-275016-ij5yaqkx	13	68	equine	equine	JJ
cord-275016-ij5yaqkx	13	69	arteritis	arteritis	NN
cord-275016-ij5yaqkx	13	70	virus	virus	NN
cord-275016-ij5yaqkx	13	71	(	(	-LRB-
cord-275016-ij5yaqkx	13	72	Barrette	Barrette	NNP
cord-275016-ij5yaqkx	13	73	-	-	HYPH
cord-275016-ij5yaqkx	13	74	Ng	Ng	NNP
cord-275016-ij5yaqkx	13	75	et	et	FW
cord-275016-ij5yaqkx	13	76	al	al	NNP
cord-275016-ij5yaqkx	13	77	.	.	NNP
cord-275016-ij5yaqkx	13	78	,	,	,
cord-275016-ij5yaqkx	13	79	2002	2002	CD
cord-275016-ij5yaqkx	13	80	)	)	-RRB-
cord-275016-ij5yaqkx	13	81	.	.	.
cord-275016-ij5yaqkx	14	1	These	these	DT
cord-275016-ij5yaqkx	14	2	studies	study	NNS
cord-275016-ij5yaqkx	14	3	allowed	allow	VBD
cord-275016-ij5yaqkx	14	4	us	-PRON-	PRP
cord-275016-ij5yaqkx	14	5	to	to	TO
cord-275016-ij5yaqkx	14	6	identify	identify	VB
cord-275016-ij5yaqkx	14	7	the	the	DT
cord-275016-ij5yaqkx	14	8	active	active	JJ
cord-275016-ij5yaqkx	14	9	-	-	HYPH
cord-275016-ij5yaqkx	14	10	site	site	NN
cord-275016-ij5yaqkx	14	11	amino	amino	NN
cord-275016-ij5yaqkx	14	12	acid	acid	NN
cord-275016-ij5yaqkx	14	13	residues	residue	NNS
cord-275016-ij5yaqkx	14	14	with	with	IN
cord-275016-ij5yaqkx	14	15	a	a	DT
cord-275016-ij5yaqkx	14	16	combination	combination	NN
cord-275016-ij5yaqkx	14	17	of	of	IN
cord-275016-ij5yaqkx	14	18	site	site	NN
cord-275016-ij5yaqkx	14	19	-	-	HYPH
cord-275016-ij5yaqkx	14	20	directed	direct	VBN
cord-275016-ij5yaqkx	14	21	mutagenesis	mutagenesis	NN
cord-275016-ij5yaqkx	14	22	studies	study	NNS
cord-275016-ij5yaqkx	14	23	(	(	-LRB-
cord-275016-ij5yaqkx	14	24	Boniotti	Boniotti	NNP
cord-275016-ij5yaqkx	14	25	et	et	NNP
cord-275016-ij5yaqkx	14	26	al	al	NNP
cord-275016-ij5yaqkx	14	27	.	.	NNP
cord-275016-ij5yaqkx	14	28	,	,	,
cord-275016-ij5yaqkx	14	29	1994	1994	CD
cord-275016-ij5yaqkx	14	30	;	;	:
cord-275016-ij5yaqkx	14	31	Cheah	Cheah	NNP
cord-275016-ij5yaqkx	14	32	et	et	NNP
cord-275016-ij5yaqkx	14	33	al	al	NNP
cord-275016-ij5yaqkx	14	34	.	.	NNP
cord-275016-ij5yaqkx	14	35	,	,	,
cord-275016-ij5yaqkx	14	36	1990	1990	CD
cord-275016-ij5yaqkx	14	37	;	;	:
cord-275016-ij5yaqkx	14	38	Gosert	Gosert	NNP
cord-275016-ij5yaqkx	14	39	et	et	NNP
cord-275016-ij5yaqkx	14	40	al	al	NNP
cord-275016-ij5yaqkx	14	41	.	.	NNP
cord-275016-ij5yaqkx	14	42	,	,	,
cord-275016-ij5yaqkx	14	43	1997	1997	CD
cord-275016-ij5yaqkx	14	44	;	;	:
cord-275016-ij5yaqkx	14	45	Hammerle	Hammerle	NNP
cord-275016-ij5yaqkx	14	46	et	et	NNP
cord-275016-ij5yaqkx	14	47	al	al	NNP
cord-275016-ij5yaqkx	14	48	.	.	NNP
cord-275016-ij5yaqkx	14	49	,	,	,
cord-275016-ij5yaqkx	14	50	1991	1991	CD
cord-275016-ij5yaqkx	14	51	)	)	-RRB-
cord-275016-ij5yaqkx	14	52	.	.	.
cord-275016-ij5yaqkx	15	1	Several	several	JJ
cord-275016-ij5yaqkx	15	2	3C	3c	NN
cord-275016-ij5yaqkx	15	3	and	and	CC
cord-275016-ij5yaqkx	15	4	3C	3c	NN
cord-275016-ij5yaqkx	15	5	-	-	HYPH
cord-275016-ij5yaqkx	15	6	like	like	JJ
cord-275016-ij5yaqkx	15	7	proteases	protease	NNS
cord-275016-ij5yaqkx	15	8	were	be	VBD
cord-275016-ij5yaqkx	15	9	purified	purify	VBN
cord-275016-ij5yaqkx	15	10	and	and	CC
cord-275016-ij5yaqkx	15	11	their	-PRON-	PRP$
cord-275016-ij5yaqkx	15	12	biochemical	biochemical	JJ
cord-275016-ij5yaqkx	15	13	properties	property	NNS
cord-275016-ij5yaqkx	15	14	were	be	VBD
cord-275016-ij5yaqkx	15	15	well	well	RB
cord-275016-ij5yaqkx	15	16	characterized	characterize	VBN
cord-275016-ij5yaqkx	15	17	(	(	-LRB-
cord-275016-ij5yaqkx	16	1	Baum	Baum	NNP
cord-275016-ij5yaqkx	16	2	et	et	NNP
cord-275016-ij5yaqkx	16	3	al	al	NNP
cord-275016-ij5yaqkx	16	4	.	.	NNP
cord-275016-ij5yaqkx	16	5	,	,	,
cord-275016-ij5yaqkx	16	6	1991	1991	CD
cord-275016-ij5yaqkx	16	7	;	;	:
cord-275016-ij5yaqkx	16	8	Chisholm	Chisholm	NNP
cord-275016-ij5yaqkx	16	9	et	et	NNP
cord-275016-ij5yaqkx	16	10	al	al	NNP
cord-275016-ij5yaqkx	16	11	.	.	.
cord-275016-ij5yaqkx	17	1	,	,	,
cord-275016-ij5yaqkx	17	2	2001	2001	CD
cord-275016-ij5yaqkx	17	3	;	;	:
cord-275016-ij5yaqkx	17	4	Davis	Davis	NNP
cord-275016-ij5yaqkx	17	5	et	et	NNP
cord-275016-ij5yaqkx	17	6	al	al	NNP
cord-275016-ij5yaqkx	17	7	.	.	NNP
cord-275016-ij5yaqkx	17	8	,	,	,
cord-275016-ij5yaqkx	17	9	1997	1997	CD
cord-275016-ij5yaqkx	17	10	;	;	:
cord-275016-ij5yaqkx	17	11	Ziebuhr	Ziebuhr	NNP
cord-275016-ij5yaqkx	17	12	et	et	NNP
cord-275016-ij5yaqkx	17	13	al	al	NNP
cord-275016-ij5yaqkx	17	14	.	.	NNP
cord-275016-ij5yaqkx	17	15	,	,	,
cord-275016-ij5yaqkx	17	16	1997	1997	CD
cord-275016-ij5yaqkx	17	17	)	)	-RRB-
cord-275016-ij5yaqkx	17	18	.	.	.
cord-275016-ij5yaqkx	18	1	They	-PRON-	PRP
cord-275016-ij5yaqkx	18	2	are	be	VBP
cord-275016-ij5yaqkx	18	3	characterized	characterize	VBN
cord-275016-ij5yaqkx	18	4	by	by	IN
cord-275016-ij5yaqkx	18	5	their	-PRON-	PRP$
cord-275016-ij5yaqkx	18	6	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	18	7	activities	activity	NNS
cord-275016-ij5yaqkx	18	8	being	be	VBG
cord-275016-ij5yaqkx	18	9	inhibited	inhibit	VBN
cord-275016-ij5yaqkx	18	10	by	by	IN
cord-275016-ij5yaqkx	18	11	both	both	DT
cord-275016-ij5yaqkx	18	12	cysteine	cysteine	NN
cord-275016-ij5yaqkx	18	13	and	and	CC
cord-275016-ij5yaqkx	18	14	serine	serine	NN
cord-275016-ij5yaqkx	18	15	protease	protease	NN
cord-275016-ij5yaqkx	18	16	inhibitors	inhibitor	NNS
cord-275016-ij5yaqkx	18	17	.	.	.
cord-275016-ij5yaqkx	19	1	As	as	IN
cord-275016-ij5yaqkx	19	2	a	a	DT
cord-275016-ij5yaqkx	19	3	result	result	NN
cord-275016-ij5yaqkx	19	4	of	of	IN
cord-275016-ij5yaqkx	19	5	these	these	DT
cord-275016-ij5yaqkx	19	6	studies	study	NNS
cord-275016-ij5yaqkx	19	7	,	,	,
cord-275016-ij5yaqkx	19	8	therapeutic	therapeutic	JJ
cord-275016-ij5yaqkx	19	9	drugs	drug	NNS
cord-275016-ij5yaqkx	19	10	,	,	,
cord-275016-ij5yaqkx	19	11	especially	especially	RB
cord-275016-ij5yaqkx	19	12	for	for	IN
cord-275016-ij5yaqkx	19	13	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	19	14	,	,	,
cord-275016-ij5yaqkx	19	15	are	be	VBP
cord-275016-ij5yaqkx	19	16	being	be	VBG
cord-275016-ij5yaqkx	19	17	developed	develop	VBN
cord-275016-ij5yaqkx	19	18	(	(	-LRB-
cord-275016-ij5yaqkx	19	19	Hammerle	Hammerle	NNP
cord-275016-ij5yaqkx	19	20	et	et	NNP
cord-275016-ij5yaqkx	19	21	al	al	NNP
cord-275016-ij5yaqkx	19	22	.	.	NNP
cord-275016-ij5yaqkx	19	23	,	,	,
cord-275016-ij5yaqkx	19	24	1991	1991	CD
cord-275016-ij5yaqkx	19	25	;	;	:
cord-275016-ij5yaqkx	19	26	McKinlay	McKinlay	NNP
cord-275016-ij5yaqkx	19	27	,	,	,
cord-275016-ij5yaqkx	19	28	2001	2001	CD
cord-275016-ij5yaqkx	19	29	;	;	:
cord-275016-ij5yaqkx	19	30	Turner	Turner	NNP
cord-275016-ij5yaqkx	19	31	,	,	,
cord-275016-ij5yaqkx	19	32	2001	2001	CD
cord-275016-ij5yaqkx	19	33	)	)	-RRB-
cord-275016-ij5yaqkx	19	34	.	.	.
cord-275016-ij5yaqkx	20	1	Norovirus	norovirus	NN
cord-275016-ij5yaqkx	20	2	(	(	-LRB-
cord-275016-ij5yaqkx	20	3	formerly	formerly	RB
cord-275016-ij5yaqkx	20	4	Norwalk	Norwalk	NNP
cord-275016-ij5yaqkx	20	5	-	-	HYPH
cord-275016-ij5yaqkx	20	6	like	like	JJ
cord-275016-ij5yaqkx	20	7	virus	virus	NN
cord-275016-ij5yaqkx	20	8	)	)	-RRB-
cord-275016-ij5yaqkx	20	9	is	be	VBZ
cord-275016-ij5yaqkx	20	10	a	a	DT
cord-275016-ij5yaqkx	20	11	genus	genus	NN
cord-275016-ij5yaqkx	20	12	of	of	IN
cord-275016-ij5yaqkx	20	13	the	the	DT
cord-275016-ij5yaqkx	20	14	family	family	NN
cord-275016-ij5yaqkx	20	15	Caliciviridae	Caliciviridae	NNP
cord-275016-ij5yaqkx	20	16	and	and	CC
cord-275016-ij5yaqkx	20	17	is	be	VBZ
cord-275016-ij5yaqkx	20	18	a	a	DT
cord-275016-ij5yaqkx	20	19	major	major	JJ
cord-275016-ij5yaqkx	20	20	causative	causative	JJ
cord-275016-ij5yaqkx	20	21	agent	agent	NN
cord-275016-ij5yaqkx	20	22	of	of	IN
cord-275016-ij5yaqkx	20	23	nonbacterial	nonbacterial	JJ
cord-275016-ij5yaqkx	20	24	acute	acute	JJ
cord-275016-ij5yaqkx	20	25	gastroenteritis	gastroenteritis	NN
cord-275016-ij5yaqkx	20	26	in	in	IN
cord-275016-ij5yaqkx	20	27	humans	human	NNS
cord-275016-ij5yaqkx	21	1	(	(	-LRB-
cord-275016-ij5yaqkx	21	2	Clarke	Clarke	NNP
cord-275016-ij5yaqkx	21	3	et	et	NNP
cord-275016-ij5yaqkx	21	4	al	al	NNP
cord-275016-ij5yaqkx	21	5	.	.	NNP
cord-275016-ij5yaqkx	21	6	,	,	,
cord-275016-ij5yaqkx	21	7	1998	1998	CD
cord-275016-ij5yaqkx	21	8	;	;	:
cord-275016-ij5yaqkx	22	1	Estes	Estes	NNP
cord-275016-ij5yaqkx	22	2	et	et	NNP
cord-275016-ij5yaqkx	22	3	al	al	NNP
cord-275016-ij5yaqkx	22	4	.	.	NNP
cord-275016-ij5yaqkx	22	5	,	,	,
cord-275016-ij5yaqkx	22	6	1997	1997	CD
cord-275016-ij5yaqkx	22	7	)	)	-RRB-
cord-275016-ij5yaqkx	22	8	.	.	.
cord-275016-ij5yaqkx	23	1	Norovirus	norovirus	NN
cord-275016-ij5yaqkx	23	2	can	can	MD
cord-275016-ij5yaqkx	23	3	not	not	RB
cord-275016-ij5yaqkx	23	4	be	be	VB
cord-275016-ij5yaqkx	23	5	propagated	propagate	VBN
cord-275016-ij5yaqkx	23	6	in	in	IN
cord-275016-ij5yaqkx	23	7	cell	cell	NN
cord-275016-ij5yaqkx	23	8	cultures	culture	NNS
cord-275016-ij5yaqkx	23	9	,	,	,
cord-275016-ij5yaqkx	23	10	and	and	CC
cord-275016-ij5yaqkx	23	11	animal	animal	NN
cord-275016-ij5yaqkx	23	12	models	model	NNS
cord-275016-ij5yaqkx	23	13	have	have	VBP
cord-275016-ij5yaqkx	23	14	not	not	RB
cord-275016-ij5yaqkx	23	15	been	be	VBN
cord-275016-ij5yaqkx	23	16	developed	develop	VBN
cord-275016-ij5yaqkx	23	17	,	,	,
cord-275016-ij5yaqkx	23	18	which	which	WDT
cord-275016-ij5yaqkx	23	19	hampers	hamper	VBZ
cord-275016-ij5yaqkx	23	20	the	the	DT
cord-275016-ij5yaqkx	23	21	progress	progress	NN
cord-275016-ij5yaqkx	23	22	of	of	IN
cord-275016-ij5yaqkx	23	23	biochemical	biochemical	JJ
cord-275016-ij5yaqkx	23	24	and	and	CC
cord-275016-ij5yaqkx	23	25	molecular	molecular	JJ
cord-275016-ij5yaqkx	23	26	biological	biological	JJ
cord-275016-ij5yaqkx	23	27	studies	study	NNS
cord-275016-ij5yaqkx	23	28	of	of	IN
cord-275016-ij5yaqkx	23	29	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	23	30	.	.	.
cord-275016-ij5yaqkx	24	1	We	-PRON-	PRP
cord-275016-ij5yaqkx	24	2	cloned	clone	VBD
cord-275016-ij5yaqkx	24	3	and	and	CC
cord-275016-ij5yaqkx	24	4	sequenced	sequence	VBD
cord-275016-ij5yaqkx	24	5	the	the	DT
cord-275016-ij5yaqkx	24	6	whole	whole	JJ
cord-275016-ij5yaqkx	24	7	genome	genome	NN
cord-275016-ij5yaqkx	24	8	of	of	IN
cord-275016-ij5yaqkx	24	9	a	a	DT
cord-275016-ij5yaqkx	24	10	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	24	11	strain	strain	NN
cord-275016-ij5yaqkx	24	12	of	of	IN
cord-275016-ij5yaqkx	24	13	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	24	14	(	(	-LRB-
cord-275016-ij5yaqkx	24	15	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	24	16	virus	virus	NN
cord-275016-ij5yaqkx	24	17	)	)	-RRB-
cord-275016-ij5yaqkx	24	18	,	,	,
cord-275016-ij5yaqkx	24	19	and	and	CC
cord-275016-ij5yaqkx	24	20	its	-PRON-	PRP$
cord-275016-ij5yaqkx	24	21	3C	3c	NN
cord-275016-ij5yaqkx	24	22	-	-	HYPH
cord-275016-ij5yaqkx	24	23	like	like	JJ
cord-275016-ij5yaqkx	24	24	protease	protease	NN
cord-275016-ij5yaqkx	24	25	has	have	VBZ
cord-275016-ij5yaqkx	24	26	been	be	VBN
cord-275016-ij5yaqkx	24	27	heterologously	heterologously	RB
cord-275016-ij5yaqkx	24	28	expressed	express	VBN
cord-275016-ij5yaqkx	24	29	in	in	IN
cord-275016-ij5yaqkx	24	30	Escherichia	Escherichia	NNP
cord-275016-ij5yaqkx	24	31	coli	coli	NNS
cord-275016-ij5yaqkx	24	32	in	in	IN
cord-275016-ij5yaqkx	24	33	an	an	DT
cord-275016-ij5yaqkx	24	34	active	active	JJ
cord-275016-ij5yaqkx	24	35	form	form	NN
cord-275016-ij5yaqkx	24	36	(	(	-LRB-
cord-275016-ij5yaqkx	24	37	Someya	Someya	NNP
cord-275016-ij5yaqkx	24	38	et	et	NNP
cord-275016-ij5yaqkx	24	39	al	al	NNP
cord-275016-ij5yaqkx	24	40	.	.	NNP
cord-275016-ij5yaqkx	24	41	,	,	,
cord-275016-ij5yaqkx	24	42	2000	2000	CD
cord-275016-ij5yaqkx	24	43	)	)	-RRB-
cord-275016-ij5yaqkx	24	44	.	.	.
cord-275016-ij5yaqkx	25	1	Furthermore	furthermore	RB
cord-275016-ij5yaqkx	25	2	,	,	,
cord-275016-ij5yaqkx	25	3	we	-PRON-	PRP
cord-275016-ij5yaqkx	25	4	identified	identify	VBD
cord-275016-ij5yaqkx	25	5	the	the	DT
cord-275016-ij5yaqkx	25	6	active	active	JJ
cord-275016-ij5yaqkx	25	7	-	-	HYPH
cord-275016-ij5yaqkx	25	8	site	site	NN
cord-275016-ij5yaqkx	25	9	amino	amino	NN
cord-275016-ij5yaqkx	25	10	acid	acid	NN
cord-275016-ij5yaqkx	25	11	residues	residue	NNS
cord-275016-ij5yaqkx	25	12	and	and	CC
cord-275016-ij5yaqkx	25	13	concluded	conclude	VBD
cord-275016-ij5yaqkx	25	14	that	that	IN
cord-275016-ij5yaqkx	25	15	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	25	16	3C	3c	NN
cord-275016-ij5yaqkx	25	17	-	-	HYPH
cord-275016-ij5yaqkx	25	18	like	like	JJ
cord-275016-ij5yaqkx	25	19	protease	protease	NN
cord-275016-ij5yaqkx	25	20	had	have	VBD
cord-275016-ij5yaqkx	25	21	a	a	DT
cord-275016-ij5yaqkx	25	22	catalytic	catalytic	JJ
cord-275016-ij5yaqkx	25	23	dyad	dyad	NN
cord-275016-ij5yaqkx	25	24	consisting	consist	VBG
cord-275016-ij5yaqkx	25	25	of	of	IN
cord-275016-ij5yaqkx	25	26	His30	His30	NNP
cord-275016-ij5yaqkx	25	27	and	and	CC
cord-275016-ij5yaqkx	25	28	Cys139	cys139	CD
cord-275016-ij5yaqkx	25	29	(	(	-LRB-
cord-275016-ij5yaqkx	25	30	Someya	Someya	NNP
cord-275016-ij5yaqkx	25	31	et	et	NNP
cord-275016-ij5yaqkx	25	32	al	al	NNP
cord-275016-ij5yaqkx	25	33	.	.	NNP
cord-275016-ij5yaqkx	25	34	,	,	,
cord-275016-ij5yaqkx	25	35	2002	2002	CD
cord-275016-ij5yaqkx	25	36	)	)	-RRB-
cord-275016-ij5yaqkx	25	37	.	.	.
cord-275016-ij5yaqkx	26	1	This	this	DT
cord-275016-ij5yaqkx	26	2	is	be	VBZ
cord-275016-ij5yaqkx	26	3	a	a	DT
cord-275016-ij5yaqkx	26	4	unique	unique	JJ
cord-275016-ij5yaqkx	26	5	feature	feature	NN
cord-275016-ij5yaqkx	26	6	since	since	IN
cord-275016-ij5yaqkx	26	7	most	most	JJS
cord-275016-ij5yaqkx	26	8	of	of	IN
cord-275016-ij5yaqkx	26	9	the	the	DT
cord-275016-ij5yaqkx	26	10	3C	3C	NNP
cord-275016-ij5yaqkx	26	11	and	and	CC
cord-275016-ij5yaqkx	26	12	3C	3C	NNP
cord-275016-ij5yaqkx	26	13	-	-	HYPH
cord-275016-ij5yaqkx	26	14	like	like	JJ
cord-275016-ij5yaqkx	26	15	proteases	protease	NNS
cord-275016-ij5yaqkx	26	16	have	have	VBP
cord-275016-ij5yaqkx	26	17	a	a	DT
cord-275016-ij5yaqkx	26	18	catalytic	catalytic	JJ
cord-275016-ij5yaqkx	26	19	triad	triad	NN
cord-275016-ij5yaqkx	26	20	consisting	consist	VBG
cord-275016-ij5yaqkx	26	21	of	of	IN
cord-275016-ij5yaqkx	26	22	His	-PRON-	PRP$
cord-275016-ij5yaqkx	26	23	,	,	,
cord-275016-ij5yaqkx	26	24	Asp	Asp	NNP
cord-275016-ij5yaqkx	26	25	/	/	SYM
cord-275016-ij5yaqkx	26	26	Glu	Glu	NNP
cord-275016-ij5yaqkx	26	27	,	,	,
cord-275016-ij5yaqkx	26	28	and	and	CC
cord-275016-ij5yaqkx	26	29	Cys	Cys	NNP
cord-275016-ij5yaqkx	26	30	/	/	SYM
cord-275016-ij5yaqkx	26	31	Ser	Ser	NNP
cord-275016-ij5yaqkx	26	32	,	,	,
cord-275016-ij5yaqkx	26	33	similar	similar	JJ
cord-275016-ij5yaqkx	26	34	to	to	IN
cord-275016-ij5yaqkx	26	35	chymotrypsin	chymotrypsin	NNP
cord-275016-ij5yaqkx	26	36	(	(	-LRB-
cord-275016-ij5yaqkx	26	37	Dougherty	Dougherty	NNP
cord-275016-ij5yaqkx	26	38	and	and	CC
cord-275016-ij5yaqkx	26	39	Semler	Semler	NNP
cord-275016-ij5yaqkx	26	40	,	,	,
cord-275016-ij5yaqkx	26	41	1993	1993	CD
cord-275016-ij5yaqkx	26	42	;	;	:
cord-275016-ij5yaqkx	27	1	Gorbalenya	Gorbalenya	NNP
cord-275016-ij5yaqkx	27	2	et	et	NNP
cord-275016-ij5yaqkx	27	3	al	al	NNP
cord-275016-ij5yaqkx	27	4	.	.	.
cord-275016-ij5yaqkx	28	1	,	,	,
cord-275016-ij5yaqkx	28	2	1989	1989	CD
cord-275016-ij5yaqkx	28	3	shown	show	VBN
cord-275016-ij5yaqkx	28	4	that	that	IN
cord-275016-ij5yaqkx	28	5	the	the	DT
cord-275016-ij5yaqkx	28	6	active	active	JJ
cord-275016-ij5yaqkx	28	7	site	site	NN
cord-275016-ij5yaqkx	28	8	of	of	IN
cord-275016-ij5yaqkx	28	9	Coronavirus	Coronavirus	NNP
cord-275016-ij5yaqkx	28	10	3C	3c	NN
cord-275016-ij5yaqkx	28	11	-	-	HYPH
cord-275016-ij5yaqkx	28	12	like	like	JJ
cord-275016-ij5yaqkx	28	13	protease	protease	NN
cord-275016-ij5yaqkx	28	14	was	be	VBD
cord-275016-ij5yaqkx	28	15	also	also	RB
cord-275016-ij5yaqkx	28	16	composed	compose	VBN
cord-275016-ij5yaqkx	28	17	of	of	IN
cord-275016-ij5yaqkx	28	18	two	two	CD
cord-275016-ij5yaqkx	28	19	residues	residue	NNS
cord-275016-ij5yaqkx	28	20	,	,	,
cord-275016-ij5yaqkx	28	21	His	-PRON-	PRP$
cord-275016-ij5yaqkx	28	22	and	and	CC
cord-275016-ij5yaqkx	28	23	Cys	Cys	NNP
cord-275016-ij5yaqkx	28	24	(	(	-LRB-
cord-275016-ij5yaqkx	28	25	Anand	Anand	NNP
cord-275016-ij5yaqkx	28	26	et	et	NNP
cord-275016-ij5yaqkx	28	27	al	al	NNP
cord-275016-ij5yaqkx	28	28	.	.	NNP
cord-275016-ij5yaqkx	28	29	,	,	,
cord-275016-ij5yaqkx	28	30	2002	2002	CD
cord-275016-ij5yaqkx	28	31	)	)	-RRB-
cord-275016-ij5yaqkx	28	32	.	.	.
cord-275016-ij5yaqkx	29	1	To	to	TO
cord-275016-ij5yaqkx	29	2	obtain	obtain	VB
cord-275016-ij5yaqkx	29	3	information	information	NN
cord-275016-ij5yaqkx	29	4	regarding	regard	VBG
cord-275016-ij5yaqkx	29	5	the	the	DT
cord-275016-ij5yaqkx	29	6	biochemical	biochemical	JJ
cord-275016-ij5yaqkx	29	7	properties	property	NNS
cord-275016-ij5yaqkx	29	8	of	of	IN
cord-275016-ij5yaqkx	29	9	norovirus	norovirus	NN
cord-275016-ij5yaqkx	29	10	3C	3c	NN
cord-275016-ij5yaqkx	29	11	-	-	HYPH
cord-275016-ij5yaqkx	29	12	like	like	JJ
cord-275016-ij5yaqkx	29	13	protease	protease	NN
cord-275016-ij5yaqkx	29	14	,	,	,
cord-275016-ij5yaqkx	29	15	we	-PRON-	PRP
cord-275016-ij5yaqkx	29	16	purified	purify	VBD
cord-275016-ij5yaqkx	29	17	the	the	DT
cord-275016-ij5yaqkx	29	18	bacterially	bacterially	RB
cord-275016-ij5yaqkx	29	19	expressed	express	VBN
cord-275016-ij5yaqkx	29	20	recombinant	recombinant	JJ
cord-275016-ij5yaqkx	29	21	3C	3c	NN
cord-275016-ij5yaqkx	29	22	-	-	HYPH
cord-275016-ij5yaqkx	29	23	like	like	JJ
cord-275016-ij5yaqkx	29	24	protease	protease	NN
cord-275016-ij5yaqkx	29	25	from	from	IN
cord-275016-ij5yaqkx	29	26	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	29	27	virus	virus	NN
cord-275016-ij5yaqkx	29	28	and	and	CC
cord-275016-ij5yaqkx	29	29	characterized	characterize	VBD
cord-275016-ij5yaqkx	29	30	as	as	IN
cord-275016-ij5yaqkx	29	31	to	to	IN
cord-275016-ij5yaqkx	29	32	effects	effect	NNS
cord-275016-ij5yaqkx	29	33	of	of	IN
cord-275016-ij5yaqkx	29	34	pH	ph	NN
cord-275016-ij5yaqkx	29	35	,	,	,
cord-275016-ij5yaqkx	29	36	temperature	temperature	NN
cord-275016-ij5yaqkx	29	37	,	,	,
cord-275016-ij5yaqkx	29	38	salt	salt	NN
cord-275016-ij5yaqkx	29	39	contents	content	NNS
cord-275016-ij5yaqkx	29	40	,	,	,
cord-275016-ij5yaqkx	29	41	and	and	CC
cord-275016-ij5yaqkx	29	42	SH	sh	NN
cord-275016-ij5yaqkx	29	43	reagents	reagent	NNS
cord-275016-ij5yaqkx	29	44	on	on	IN
cord-275016-ij5yaqkx	29	45	its	-PRON-	PRP$
cord-275016-ij5yaqkx	29	46	activity	activity	NN
cord-275016-ij5yaqkx	29	47	.	.	.
cord-275016-ij5yaqkx	30	1	The	the	DT
cord-275016-ij5yaqkx	30	2	DNA	dna	NN
cord-275016-ij5yaqkx	30	3	fragment	fragment	NN
cord-275016-ij5yaqkx	30	4	encoding	encode	VBG
cord-275016-ij5yaqkx	30	5	all	all	DT
cord-275016-ij5yaqkx	30	6	residues	residue	NNS
cord-275016-ij5yaqkx	30	7	(	(	-LRB-
cord-275016-ij5yaqkx	30	8	Ala1	Ala1	NNP
cord-275016-ij5yaqkx	30	9	to	to	IN
cord-275016-ij5yaqkx	30	10	Glu181	Glu181	,
cord-275016-ij5yaqkx	30	11	)	)	-RRB-
cord-275016-ij5yaqkx	30	12	of	of	IN
cord-275016-ij5yaqkx	30	13	the	the	DT
cord-275016-ij5yaqkx	30	14	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	30	15	virus	virus	NN
cord-275016-ij5yaqkx	30	16	3C	3c	NN
cord-275016-ij5yaqkx	30	17	-	-	HYPH
cord-275016-ij5yaqkx	30	18	like	like	JJ
cord-275016-ij5yaqkx	30	19	protease	protease	NN
cord-275016-ij5yaqkx	30	20	was	be	VBD
cord-275016-ij5yaqkx	30	21	amplified	amplify	VBN
cord-275016-ij5yaqkx	30	22	by	by	IN
cord-275016-ij5yaqkx	30	23	PCR	PCR	NNP
cord-275016-ij5yaqkx	30	24	,	,	,
cord-275016-ij5yaqkx	30	25	in	in	IN
cord-275016-ij5yaqkx	30	26	that	that	DT
cord-275016-ij5yaqkx	30	27	NdeI	NdeI	NNP
cord-275016-ij5yaqkx	30	28	and	and	CC
cord-275016-ij5yaqkx	30	29	Aor51HI	Aor51HI	NNP
cord-275016-ij5yaqkx	30	30	restriction	restriction	NN
cord-275016-ij5yaqkx	30	31	sites	site	NNS
cord-275016-ij5yaqkx	30	32	were	be	VBD
cord-275016-ij5yaqkx	30	33	introduced	introduce	VBN
cord-275016-ij5yaqkx	30	34	at	at	IN
cord-275016-ij5yaqkx	30	35	the	the	DT
cord-275016-ij5yaqkx	30	36	5	5	CD
cord-275016-ij5yaqkx	30	37	and	and	CC
cord-275016-ij5yaqkx	30	38	3	3	CD
cord-275016-ij5yaqkx	30	39	ends	end	NNS
cord-275016-ij5yaqkx	30	40	,	,	,
cord-275016-ij5yaqkx	30	41	respectively	respectively	RB
cord-275016-ij5yaqkx	30	42	.	.	.
cord-275016-ij5yaqkx	31	1	The	the	DT
cord-275016-ij5yaqkx	31	2	NdeI	NdeI	NNP
cord-275016-ij5yaqkx	31	3	-	-	HYPH
cord-275016-ij5yaqkx	31	4	Aor51HI	Aor51HI	NNP
cord-275016-ij5yaqkx	31	5	fragment	fragment	NN
cord-275016-ij5yaqkx	31	6	was	be	VBD
cord-275016-ij5yaqkx	31	7	placed	place	VBN
cord-275016-ij5yaqkx	31	8	downstream	downstream	RB
cord-275016-ij5yaqkx	31	9	of	of	IN
cord-275016-ij5yaqkx	31	10	the	the	DT
cord-275016-ij5yaqkx	31	11	T7	T7	NNP
cord-275016-ij5yaqkx	31	12	promoter	promoter	NN
cord-275016-ij5yaqkx	31	13	with	with	IN
cord-275016-ij5yaqkx	31	14	a	a	DT
cord-275016-ij5yaqkx	31	15	linker	linker	NN
cord-275016-ij5yaqkx	31	16	encoding	encode	VBG
cord-275016-ij5yaqkx	31	17	a	a	DT
cord-275016-ij5yaqkx	31	18	sequence	sequence	NN
cord-275016-ij5yaqkx	31	19	containing	contain	VBG
cord-275016-ij5yaqkx	31	20	six	six	CD
cord-275016-ij5yaqkx	31	21	consecutive	consecutive	JJ
cord-275016-ij5yaqkx	31	22	His	-PRON-	PRP$
cord-275016-ij5yaqkx	31	23	residues	residue	NNS
cord-275016-ij5yaqkx	31	24	.	.	.
cord-275016-ij5yaqkx	32	1	The	the	DT
cord-275016-ij5yaqkx	32	2	resultant	resultant	JJ
cord-275016-ij5yaqkx	32	3	plasmid	plasmid	NN
cord-275016-ij5yaqkx	32	4	,	,	,
cord-275016-ij5yaqkx	32	5	pT7ProHis	pT7ProHis	NNP
cord-275016-ij5yaqkx	32	6	,	,	,
cord-275016-ij5yaqkx	32	7	encodes	encode	VBZ
cord-275016-ij5yaqkx	32	8	a	a	DT
cord-275016-ij5yaqkx	32	9	3Clike	3clike	JJ
cord-275016-ij5yaqkx	32	10	protease	protease	NN
cord-275016-ij5yaqkx	32	11	with	with	IN
cord-275016-ij5yaqkx	32	12	a	a	DT
cord-275016-ij5yaqkx	32	13	Met	Met	NNP
cord-275016-ij5yaqkx	32	14	residue	residue	NN
cord-275016-ij5yaqkx	32	15	in	in	IN
cord-275016-ij5yaqkx	32	16	the	the	DT
cord-275016-ij5yaqkx	32	17	N	N	NNP
cord-275016-ij5yaqkx	32	18	-	-	HYPH
cord-275016-ij5yaqkx	32	19	terminal	terminal	NN
cord-275016-ij5yaqkx	32	20	and	and	CC
cord-275016-ij5yaqkx	32	21	with	with	IN
cord-275016-ij5yaqkx	32	22	a	a	DT
cord-275016-ij5yaqkx	32	23	SAGHHHHHHG	SAGHHHHHHG	NNP
cord-275016-ij5yaqkx	32	24	sequence	sequence	NN
cord-275016-ij5yaqkx	32	25	in	in	IN
cord-275016-ij5yaqkx	32	26	the	the	DT
cord-275016-ij5yaqkx	32	27	C	c	NN
cord-275016-ij5yaqkx	32	28	-	-	HYPH
cord-275016-ij5yaqkx	32	29	terminal	terminal	NN
cord-275016-ij5yaqkx	32	30	.	.	.
cord-275016-ij5yaqkx	33	1	The	the	DT
cord-275016-ij5yaqkx	33	2	NdeI	NdeI	NNP
cord-275016-ij5yaqkx	33	3	-	-	HYPH
cord-275016-ij5yaqkx	33	4	SphI	SphI	NNP
cord-275016-ij5yaqkx	33	5	fragment	fragment	NN
cord-275016-ij5yaqkx	33	6	from	from	IN
cord-275016-ij5yaqkx	34	1	pUCHis3CD	pUCHis3CD	NNP
cord-275016-ij5yaqkx	34	2	(	(	-LRB-
cord-275016-ij5yaqkx	34	3	Someya	Someya	NNP
cord-275016-ij5yaqkx	34	4	et	et	NNP
cord-275016-ij5yaqkx	34	5	al	al	NNP
cord-275016-ij5yaqkx	34	6	.	.	.
cord-275016-ij5yaqkx	35	1	,	,	,
cord-275016-ij5yaqkx	35	2	2000	2000	CD
cord-275016-ij5yaqkx	35	3	)	)	-RRB-
cord-275016-ij5yaqkx	35	4	encoding	encode	VBG
cord-275016-ij5yaqkx	35	5	the	the	DT
cord-275016-ij5yaqkx	35	6	3CD	3cd	CD
cord-275016-ij5yaqkx	35	7	region	region	NN
cord-275016-ij5yaqkx	35	8	was	be	VBD
cord-275016-ij5yaqkx	35	9	placed	place	VBN
cord-275016-ij5yaqkx	35	10	downstream	downstream	RB
cord-275016-ij5yaqkx	35	11	of	of	IN
cord-275016-ij5yaqkx	35	12	the	the	DT
cord-275016-ij5yaqkx	35	13	T7	T7	NNP
cord-275016-ij5yaqkx	35	14	promoter	promoter	NN
cord-275016-ij5yaqkx	35	15	to	to	TO
cord-275016-ij5yaqkx	35	16	construct	construct	VB
cord-275016-ij5yaqkx	35	17	pT7His3CD	pt7his3cd	NN
cord-275016-ij5yaqkx	35	18	.	.	.
cord-275016-ij5yaqkx	36	1	Next	next	RB
cord-275016-ij5yaqkx	36	2	,	,	,
cord-275016-ij5yaqkx	36	3	the	the	DT
cord-275016-ij5yaqkx	36	4	C139A	C139A	NNP
cord-275016-ij5yaqkx	36	5	mutation	mutation	NN
cord-275016-ij5yaqkx	36	6	of	of	IN
cord-275016-ij5yaqkx	36	7	the	the	DT
cord-275016-ij5yaqkx	36	8	3C	3c	NN
cord-275016-ij5yaqkx	36	9	-	-	HYPH
cord-275016-ij5yaqkx	36	10	like	like	JJ
cord-275016-ij5yaqkx	36	11	protease	protease	NN
cord-275016-ij5yaqkx	36	12	was	be	VBD
cord-275016-ij5yaqkx	36	13	introduced	introduce	VBN
cord-275016-ij5yaqkx	36	14	and	and	CC
cord-275016-ij5yaqkx	36	15	designated	designate	VBN
cord-275016-ij5yaqkx	36	16	pT7His3CD	pt7his3cd	NN
cord-275016-ij5yaqkx	36	17	-	-	HYPH
cord-275016-ij5yaqkx	36	18	C139A.	C139A.	NNP
cord-275016-ij5yaqkx	37	1	The	the	DT
cord-275016-ij5yaqkx	37	2	DNA	dna	NN
cord-275016-ij5yaqkx	37	3	fragment	fragment	NN
cord-275016-ij5yaqkx	37	4	encoding	encode	VBG
cord-275016-ij5yaqkx	37	5	glutathione	glutathione	NN
cord-275016-ij5yaqkx	37	6	S	s	NN
cord-275016-ij5yaqkx	37	7	-	-	HYPH
cord-275016-ij5yaqkx	37	8	transferase	transferase	NN
cord-275016-ij5yaqkx	37	9	(	(	-LRB-
cord-275016-ij5yaqkx	37	10	GST	GST	NNP
cord-275016-ij5yaqkx	37	11	)	)	-RRB-
cord-275016-ij5yaqkx	37	12	was	be	VBD
cord-275016-ij5yaqkx	37	13	amplified	amplify	VBN
cord-275016-ij5yaqkx	37	14	by	by	IN
cord-275016-ij5yaqkx	37	15	PCR	PCR	NNP
cord-275016-ij5yaqkx	37	16	,	,	,
cord-275016-ij5yaqkx	37	17	in	in	IN
cord-275016-ij5yaqkx	37	18	that	that	DT
cord-275016-ij5yaqkx	37	19	ApaI	ApaI	NNP
cord-275016-ij5yaqkx	37	20	and	and	CC
cord-275016-ij5yaqkx	37	21	SphI	SphI	NNP
cord-275016-ij5yaqkx	37	22	restriction	restriction	NN
cord-275016-ij5yaqkx	37	23	sites	site	NNS
cord-275016-ij5yaqkx	37	24	were	be	VBD
cord-275016-ij5yaqkx	37	25	introduced	introduce	VBN
cord-275016-ij5yaqkx	37	26	at	at	IN
cord-275016-ij5yaqkx	37	27	the	the	DT
cord-275016-ij5yaqkx	37	28	5	5	CD
cord-275016-ij5yaqkx	37	29	and	and	CC
cord-275016-ij5yaqkx	37	30	3	3	CD
cord-275016-ij5yaqkx	37	31	ends	end	NNS
cord-275016-ij5yaqkx	37	32	,	,	,
cord-275016-ij5yaqkx	37	33	respectively	respectively	RB
cord-275016-ij5yaqkx	37	34	.	.	.
cord-275016-ij5yaqkx	38	1	The	the	DT
cord-275016-ij5yaqkx	38	2	ApaI	ApaI	NNP
cord-275016-ij5yaqkx	38	3	-	-	HYPH
cord-275016-ij5yaqkx	38	4	SphI	SphI	NNP
cord-275016-ij5yaqkx	38	5	fragment	fragment	NN
cord-275016-ij5yaqkx	38	6	encoding	encode	VBG
cord-275016-ij5yaqkx	38	7	GST	GST	NNP
cord-275016-ij5yaqkx	38	8	was	be	VBD
cord-275016-ij5yaqkx	38	9	ligated	ligate	VBN
cord-275016-ij5yaqkx	38	10	to	to	IN
cord-275016-ij5yaqkx	38	11	the	the	DT
cord-275016-ij5yaqkx	38	12	ApaI	ApaI	NNP
cord-275016-ij5yaqkx	38	13	-	-	HYPH
cord-275016-ij5yaqkx	38	14	SphI	SphI	NNP
cord-275016-ij5yaqkx	38	15	fragment	fragment	NN
cord-275016-ij5yaqkx	38	16	of	of	IN
cord-275016-ij5yaqkx	38	17	pT7His3CD	pt7his3cd	NN
cord-275016-ij5yaqkx	38	18	-	-	HYPH
cord-275016-ij5yaqkx	38	19	C139A.	C139A.	NNP
cord-275016-ij5yaqkx	39	1	The	the	DT
cord-275016-ij5yaqkx	39	2	resultant	resultant	JJ
cord-275016-ij5yaqkx	39	3	plasmid	plasmid	NN
cord-275016-ij5yaqkx	39	4	was	be	VBD
cord-275016-ij5yaqkx	39	5	designated	designate	VBN
cord-275016-ij5yaqkx	39	6	pT7His3Cd	pT7His3Cd	NNP
cord-275016-ij5yaqkx	39	7	-	-	HYPH
cord-275016-ij5yaqkx	39	8	GST	GST	NNP
cord-275016-ij5yaqkx	39	9	.	.	.
cord-275016-ij5yaqkx	40	1	This	this	DT
cord-275016-ij5yaqkx	40	2	plasmid	plasmid	NN
cord-275016-ij5yaqkx	40	3	encodes	encode	VBZ
cord-275016-ij5yaqkx	40	4	the	the	DT
cord-275016-ij5yaqkx	40	5	N	N	NNP
cord-275016-ij5yaqkx	40	6	-	-	HYPH
cord-275016-ij5yaqkx	40	7	terminal	terminal	NN
cord-275016-ij5yaqkx	40	8	His	-PRON-	PRP$
cord-275016-ij5yaqkx	40	9	-	-	HYPH
cord-275016-ij5yaqkx	40	10	tag	tag	NN
cord-275016-ij5yaqkx	40	11	sequence	sequence	NN
cord-275016-ij5yaqkx	40	12	(	(	-LRB-
cord-275016-ij5yaqkx	40	13	MGGHHHHHHGASA	MGGHHHHHHGASA	NNP
cord-275016-ij5yaqkx	40	14	)	)	-RRB-
cord-275016-ij5yaqkx	40	15	,	,	,
cord-275016-ij5yaqkx	40	16	followed	follow	VBN
cord-275016-ij5yaqkx	40	17	by	by	IN
cord-275016-ij5yaqkx	40	18	the	the	DT
cord-275016-ij5yaqkx	40	19	whole	whole	JJ
cord-275016-ij5yaqkx	40	20	sequence	sequence	NN
cord-275016-ij5yaqkx	40	21	of	of	IN
cord-275016-ij5yaqkx	40	22	the	the	DT
cord-275016-ij5yaqkx	40	23	C139A	C139A	NNP
cord-275016-ij5yaqkx	40	24	mutant	mutant	JJ
cord-275016-ij5yaqkx	40	25	3C	3c	NN
cord-275016-ij5yaqkx	40	26	-	-	HYPH
cord-275016-ij5yaqkx	40	27	like	like	JJ
cord-275016-ij5yaqkx	40	28	protease	protease	NN
cord-275016-ij5yaqkx	40	29	and	and	CC
cord-275016-ij5yaqkx	40	30	Gly1	Gly1	NNP
cord-275016-ij5yaqkx	40	31	to	to	IN
cord-275016-ij5yaqkx	40	32	Pro19	Pro19	NNP
cord-275016-ij5yaqkx	40	33	of	of	IN
cord-275016-ij5yaqkx	40	34	3D	3D	NNP
cord-275016-ij5yaqkx	40	35	RNA	RNA	NNP
cord-275016-ij5yaqkx	40	36	-	-	HYPH
cord-275016-ij5yaqkx	40	37	dependent	dependent	JJ
cord-275016-ij5yaqkx	40	38	RNA	RNA	NNP
cord-275016-ij5yaqkx	40	39	polymerase	polymerase	NN
cord-275016-ij5yaqkx	40	40	,	,	,
cord-275016-ij5yaqkx	40	41	and	and	CC
cord-275016-ij5yaqkx	40	42	then	then	RB
cord-275016-ij5yaqkx	40	43	followed	follow	VBN
cord-275016-ij5yaqkx	40	44	by	by	IN
cord-275016-ij5yaqkx	40	45	GST	GST	NNP
cord-275016-ij5yaqkx	40	46	.	.	.
cord-275016-ij5yaqkx	41	1	E.	E.	NNP
cord-275016-ij5yaqkx	41	2	coli	coli	NNS
cord-275016-ij5yaqkx	42	1	BL21-CodonPlus	bl21-codonplus	JJ
cord-275016-ij5yaqkx	42	2	(	(	-LRB-
cord-275016-ij5yaqkx	42	3	DE3)-RIL	de3)-ril	NN
cord-275016-ij5yaqkx	42	4	cells	cell	NNS
cord-275016-ij5yaqkx	42	5	(	(	-LRB-
cord-275016-ij5yaqkx	42	6	Stratagene	Stratagene	NNP
cord-275016-ij5yaqkx	42	7	,	,	,
cord-275016-ij5yaqkx	42	8	La	La	NNP
cord-275016-ij5yaqkx	42	9	Jolla	Jolla	NNP
cord-275016-ij5yaqkx	42	10	,	,	,
cord-275016-ij5yaqkx	42	11	CA	CA	NNP
cord-275016-ij5yaqkx	42	12	)	)	-RRB-
cord-275016-ij5yaqkx	42	13	were	be	VBD
cord-275016-ij5yaqkx	42	14	transformed	transform	VBN
cord-275016-ij5yaqkx	42	15	with	with	IN
cord-275016-ij5yaqkx	42	16	pT7ProHis	pt7prohis	NN
cord-275016-ij5yaqkx	42	17	or	or	CC
cord-275016-ij5yaqkx	42	18	pT7His3Cd	pt7his3cd	NN
cord-275016-ij5yaqkx	42	19	-	-	HYPH
cord-275016-ij5yaqkx	42	20	GST	gst	NN
cord-275016-ij5yaqkx	42	21	.	.	.
cord-275016-ij5yaqkx	43	1	Cells	cell	NNS
cord-275016-ij5yaqkx	43	2	were	be	VBD
cord-275016-ij5yaqkx	43	3	grown	grow	VBN
cord-275016-ij5yaqkx	43	4	on	on	IN
cord-275016-ij5yaqkx	43	5	medium	medium	JJ
cord-275016-ij5yaqkx	43	6	A	a	NN
cord-275016-ij5yaqkx	44	1	(	(	-LRB-
cord-275016-ij5yaqkx	44	2	McMurry	McMurry	NNP
cord-275016-ij5yaqkx	44	3	et	et	NNP
cord-275016-ij5yaqkx	44	4	al	al	NNP
cord-275016-ij5yaqkx	44	5	.	.	NNP
cord-275016-ij5yaqkx	44	6	,	,	,
cord-275016-ij5yaqkx	44	7	1980	1980	CD
cord-275016-ij5yaqkx	44	8	)	)	-RRB-
cord-275016-ij5yaqkx	44	9	supplemented	supplement	VBD
cord-275016-ij5yaqkx	44	10	with	with	IN
cord-275016-ij5yaqkx	44	11	0.2	0.2	CD
cord-275016-ij5yaqkx	44	12	%	%	NN
cord-275016-ij5yaqkx	44	13	glucose	glucose	NN
cord-275016-ij5yaqkx	44	14	and	and	CC
cord-275016-ij5yaqkx	44	15	0.1	0.1	CD
cord-275016-ij5yaqkx	44	16	%	%	NN
cord-275016-ij5yaqkx	44	17	casamino	casamino	NN
cord-275016-ij5yaqkx	44	18	acids	acid	NNS
cord-275016-ij5yaqkx	44	19	at	at	IN
cord-275016-ij5yaqkx	44	20	37	37	CD
cord-275016-ij5yaqkx	44	21	•	•	NN
cord-275016-ij5yaqkx	44	22	C.	c.	NN
cord-275016-ij5yaqkx	45	1	When	when	WRB
cord-275016-ij5yaqkx	45	2	the	the	DT
cord-275016-ij5yaqkx	45	3	absorbance	absorbance	NN
cord-275016-ij5yaqkx	45	4	at	at	IN
cord-275016-ij5yaqkx	45	5	530	530	CD
cord-275016-ij5yaqkx	45	6	nm	nm	NN
cord-275016-ij5yaqkx	45	7	reached	reach	VBD
cord-275016-ij5yaqkx	45	8	0.5	0.5	CD
cord-275016-ij5yaqkx	45	9	,	,	,
cord-275016-ij5yaqkx	45	10	IPTG	IPTG	NNP
cord-275016-ij5yaqkx	45	11	was	be	VBD
cord-275016-ij5yaqkx	45	12	added	add	VBN
cord-275016-ij5yaqkx	45	13	to	to	IN
cord-275016-ij5yaqkx	45	14	a	a	DT
cord-275016-ij5yaqkx	45	15	final	final	JJ
cord-275016-ij5yaqkx	45	16	concentration	concentration	NN
cord-275016-ij5yaqkx	45	17	of	of	IN
cord-275016-ij5yaqkx	45	18	1	1	CD
cord-275016-ij5yaqkx	45	19	mM	mm	CD
cord-275016-ij5yaqkx	45	20	for	for	IN
cord-275016-ij5yaqkx	45	21	induction	induction	NN
cord-275016-ij5yaqkx	45	22	of	of	IN
cord-275016-ij5yaqkx	45	23	gene	gene	NN
cord-275016-ij5yaqkx	45	24	expression	expression	NN
cord-275016-ij5yaqkx	45	25	.	.	.
cord-275016-ij5yaqkx	46	1	After	after	IN
cord-275016-ij5yaqkx	46	2	2	2	CD
cord-275016-ij5yaqkx	46	3	h	h	NN
cord-275016-ij5yaqkx	46	4	,	,	,
cord-275016-ij5yaqkx	46	5	cells	cell	NNS
cord-275016-ij5yaqkx	46	6	were	be	VBD
cord-275016-ij5yaqkx	46	7	harvested	harvest	VBN
cord-275016-ij5yaqkx	46	8	,	,	,
cord-275016-ij5yaqkx	46	9	then	then	RB
cord-275016-ij5yaqkx	46	10	washed	wash	VBD
cord-275016-ij5yaqkx	46	11	and	and	CC
cord-275016-ij5yaqkx	46	12	resuspended	resuspend	VBD
cord-275016-ij5yaqkx	46	13	with	with	IN
cord-275016-ij5yaqkx	46	14	20	20	CD
cord-275016-ij5yaqkx	46	15	mM	mM	NNP
cord-275016-ij5yaqkx	46	16	Tris	Tris	NNP
cord-275016-ij5yaqkx	46	17	-	-	HYPH
cord-275016-ij5yaqkx	46	18	HCl	HCl	NNP
cord-275016-ij5yaqkx	46	19	(	(	-LRB-
cord-275016-ij5yaqkx	46	20	pH	pH	NNP
cord-275016-ij5yaqkx	46	21	8.0	8.0	CD
cord-275016-ij5yaqkx	46	22	)	)	-RRB-
cord-275016-ij5yaqkx	46	23	buffer	buffer	NN
cord-275016-ij5yaqkx	46	24	containing	contain	VBG
cord-275016-ij5yaqkx	46	25	0.1	0.1	CD
cord-275016-ij5yaqkx	46	26	M	M	NNP
cord-275016-ij5yaqkx	46	27	NaCl	nacl	NN
cord-275016-ij5yaqkx	46	28	.	.	.
cord-275016-ij5yaqkx	47	1	Cells	cell	NNS
cord-275016-ij5yaqkx	47	2	were	be	VBD
cord-275016-ij5yaqkx	47	3	disrupted	disrupt	VBN
cord-275016-ij5yaqkx	47	4	by	by	IN
cord-275016-ij5yaqkx	47	5	sonication	sonication	NN
cord-275016-ij5yaqkx	47	6	.	.	.
cord-275016-ij5yaqkx	48	1	After	after	IN
cord-275016-ij5yaqkx	48	2	the	the	DT
cord-275016-ij5yaqkx	48	3	removal	removal	NN
cord-275016-ij5yaqkx	48	4	of	of	IN
cord-275016-ij5yaqkx	48	5	cell	cell	NN
cord-275016-ij5yaqkx	48	6	debris	debris	NN
cord-275016-ij5yaqkx	48	7	,	,	,
cord-275016-ij5yaqkx	48	8	the	the	DT
cord-275016-ij5yaqkx	48	9	supernatant	supernatant	NN
cord-275016-ij5yaqkx	48	10	was	be	VBD
cord-275016-ij5yaqkx	48	11	subjected	subject	VBN
cord-275016-ij5yaqkx	48	12	to	to	IN
cord-275016-ij5yaqkx	48	13	ultracentrifugation	ultracentrifugation	NN
cord-275016-ij5yaqkx	48	14	(	(	-LRB-
cord-275016-ij5yaqkx	48	15	150,000	150,000	CD
cord-275016-ij5yaqkx	48	16	×	×	NN
cord-275016-ij5yaqkx	48	17	g	g	NN
cord-275016-ij5yaqkx	48	18	,	,	,
cord-275016-ij5yaqkx	48	19	1	1	CD
cord-275016-ij5yaqkx	48	20	h	h	NN
cord-275016-ij5yaqkx	48	21	)	)	-RRB-
cord-275016-ij5yaqkx	48	22	.	.	.
cord-275016-ij5yaqkx	49	1	The	the	DT
cord-275016-ij5yaqkx	49	2	resultant	resultant	JJ
cord-275016-ij5yaqkx	49	3	supernatant	supernatant	NN
cord-275016-ij5yaqkx	49	4	was	be	VBD
cord-275016-ij5yaqkx	49	5	mixed	mix	VBN
cord-275016-ij5yaqkx	49	6	with	with	IN
cord-275016-ij5yaqkx	49	7	TALON	TALON	NNP
cord-275016-ij5yaqkx	49	8	Metal	Metal	NNP
cord-275016-ij5yaqkx	49	9	Affinity	Affinity	NNP
cord-275016-ij5yaqkx	49	10	Resin	Resin	NNP
cord-275016-ij5yaqkx	49	11	(	(	-LRB-
cord-275016-ij5yaqkx	49	12	CLONTECH	CLONTECH	NNP
cord-275016-ij5yaqkx	49	13	,	,	,
cord-275016-ij5yaqkx	49	14	Palo	Palo	NNP
cord-275016-ij5yaqkx	49	15	Alto	Alto	NNP
cord-275016-ij5yaqkx	49	16	,	,	,
cord-275016-ij5yaqkx	49	17	CA	CA	NNP
cord-275016-ij5yaqkx	49	18	)	)	-RRB-
cord-275016-ij5yaqkx	49	19	equilibrated	equilibrate	VBN
cord-275016-ij5yaqkx	49	20	with	with	IN
cord-275016-ij5yaqkx	49	21	20	20	CD
cord-275016-ij5yaqkx	49	22	mM	mM	NNP
cord-275016-ij5yaqkx	49	23	Tris	Tris	NNP
cord-275016-ij5yaqkx	49	24	-	-	HYPH
cord-275016-ij5yaqkx	49	25	HCl	HCl	NNP
cord-275016-ij5yaqkx	49	26	(	(	-LRB-
cord-275016-ij5yaqkx	49	27	pH	pH	NNP
cord-275016-ij5yaqkx	49	28	8.0	8.0	CD
cord-275016-ij5yaqkx	49	29	)	)	-RRB-
cord-275016-ij5yaqkx	49	30	buffer	buffer	NN
cord-275016-ij5yaqkx	49	31	containing	contain	VBG
cord-275016-ij5yaqkx	49	32	0.1	0.1	CD
cord-275016-ij5yaqkx	49	33	M	M	NNP
cord-275016-ij5yaqkx	49	34	NaCl	nacl	NN
cord-275016-ij5yaqkx	49	35	.	.	.
cord-275016-ij5yaqkx	50	1	The	the	DT
cord-275016-ij5yaqkx	50	2	resin	resin	NN
cord-275016-ij5yaqkx	50	3	was	be	VBD
cord-275016-ij5yaqkx	50	4	washed	wash	VBN
cord-275016-ij5yaqkx	50	5	with	with	IN
cord-275016-ij5yaqkx	50	6	20	20	CD
cord-275016-ij5yaqkx	50	7	mM	mM	NNP
cord-275016-ij5yaqkx	50	8	Tris	Tris	NNP
cord-275016-ij5yaqkx	50	9	-	-	HYPH
cord-275016-ij5yaqkx	50	10	HCl	HCl	NNP
cord-275016-ij5yaqkx	50	11	(	(	-LRB-
cord-275016-ij5yaqkx	50	12	pH	pH	NNP
cord-275016-ij5yaqkx	50	13	8.0	8.0	CD
cord-275016-ij5yaqkx	50	14	)	)	-RRB-
cord-275016-ij5yaqkx	50	15	buffer	buffer	NN
cord-275016-ij5yaqkx	50	16	containing	contain	VBG
cord-275016-ij5yaqkx	50	17	0.1	0.1	CD
cord-275016-ij5yaqkx	50	18	M	M	NNP
cord-275016-ij5yaqkx	50	19	NaCl	nacl	NN
cord-275016-ij5yaqkx	50	20	and	and	CC
cord-275016-ij5yaqkx	50	21	0.1	0.1	CD
cord-275016-ij5yaqkx	50	22	M	m	CD
cord-275016-ij5yaqkx	50	23	imidazole	imidazole	NN
cord-275016-ij5yaqkx	50	24	for	for	IN
cord-275016-ij5yaqkx	50	25	removal	removal	NN
cord-275016-ij5yaqkx	50	26	of	of	IN
cord-275016-ij5yaqkx	50	27	non	non	NN
cord-275016-ij5yaqkx	50	28	-	-	JJ
cord-275016-ij5yaqkx	50	29	specifically	specifically	RB
cord-275016-ij5yaqkx	50	30	bound	bind	VBN
cord-275016-ij5yaqkx	50	31	proteins	protein	NNS
cord-275016-ij5yaqkx	50	32	.	.	.
cord-275016-ij5yaqkx	51	1	The	the	DT
cord-275016-ij5yaqkx	51	2	proteins	protein	NNS
cord-275016-ij5yaqkx	51	3	of	of	IN
cord-275016-ij5yaqkx	51	4	interest	interest	NN
cord-275016-ij5yaqkx	51	5	were	be	VBD
cord-275016-ij5yaqkx	51	6	eluted	elute	VBN
cord-275016-ij5yaqkx	51	7	with	with	IN
cord-275016-ij5yaqkx	51	8	20	20	CD
cord-275016-ij5yaqkx	51	9	mM	mM	NNP
cord-275016-ij5yaqkx	51	10	Tris	Tris	NNP
cord-275016-ij5yaqkx	51	11	-	-	HYPH
cord-275016-ij5yaqkx	51	12	HCl	HCl	NNP
cord-275016-ij5yaqkx	51	13	(	(	-LRB-
cord-275016-ij5yaqkx	51	14	pH	pH	NNP
cord-275016-ij5yaqkx	51	15	8.0	8.0	CD
cord-275016-ij5yaqkx	51	16	)	)	-RRB-
cord-275016-ij5yaqkx	51	17	buffer	buffer	NN
cord-275016-ij5yaqkx	51	18	containing	contain	VBG
cord-275016-ij5yaqkx	51	19	0.1	0.1	CD
cord-275016-ij5yaqkx	51	20	M	M	NNP
cord-275016-ij5yaqkx	51	21	NaCl	nacl	NN
cord-275016-ij5yaqkx	51	22	and	and	CC
cord-275016-ij5yaqkx	51	23	0.5	0.5	CD
cord-275016-ij5yaqkx	51	24	M	m	NN
cord-275016-ij5yaqkx	51	25	imidazole	imidazole	NN
cord-275016-ij5yaqkx	51	26	and	and	CC
cord-275016-ij5yaqkx	51	27	concentrated	concentrate	VBD
cord-275016-ij5yaqkx	51	28	using	use	VBG
cord-275016-ij5yaqkx	51	29	Ultrafree-15	Ultrafree-15	NNP
cord-275016-ij5yaqkx	51	30	unit	unit	NN
cord-275016-ij5yaqkx	51	31	with	with	IN
cord-275016-ij5yaqkx	51	32	Biomax-10	Biomax-10	NNP
cord-275016-ij5yaqkx	51	33	membrane	membrane	NN
cord-275016-ij5yaqkx	51	34	(	(	-LRB-
cord-275016-ij5yaqkx	51	35	MILLIPORE	MILLIPORE	NNP
cord-275016-ij5yaqkx	51	36	,	,	,
cord-275016-ij5yaqkx	51	37	Bedford	Bedford	NNP
cord-275016-ij5yaqkx	51	38	,	,	,
cord-275016-ij5yaqkx	51	39	MA	MA	NNP
cord-275016-ij5yaqkx	51	40	)	)	-RRB-
cord-275016-ij5yaqkx	51	41	.	.	.
cord-275016-ij5yaqkx	52	1	Proteins	protein	NNS
cord-275016-ij5yaqkx	52	2	were	be	VBD
cord-275016-ij5yaqkx	52	3	determined	determine	VBN
cord-275016-ij5yaqkx	52	4	by	by	IN
cord-275016-ij5yaqkx	52	5	the	the	DT
cord-275016-ij5yaqkx	52	6	method	method	NN
cord-275016-ij5yaqkx	52	7	of	of	IN
cord-275016-ij5yaqkx	52	8	Bradford	Bradford	NNP
cord-275016-ij5yaqkx	52	9	(	(	-LRB-
cord-275016-ij5yaqkx	52	10	Bradford	Bradford	NNP
cord-275016-ij5yaqkx	52	11	,	,	,
cord-275016-ij5yaqkx	52	12	1976	1976	CD
cord-275016-ij5yaqkx	52	13	)	)	-RRB-
cord-275016-ij5yaqkx	52	14	.	.	.
cord-275016-ij5yaqkx	53	1	Usually	usually	RB
cord-275016-ij5yaqkx	53	2	,	,	,
cord-275016-ij5yaqkx	53	3	5	5	CD
cord-275016-ij5yaqkx	53	4	M	M	NNP
cord-275016-ij5yaqkx	53	5	enzyme	enzyme	NN
cord-275016-ij5yaqkx	53	6	and	and	CC
cord-275016-ij5yaqkx	53	7	5	5	CD
cord-275016-ij5yaqkx	53	8	M	m	NN
cord-275016-ij5yaqkx	53	9	substrate	substrate	NN
cord-275016-ij5yaqkx	53	10	were	be	VBD
cord-275016-ij5yaqkx	53	11	reacted	react	VBN
cord-275016-ij5yaqkx	53	12	in	in	IN
cord-275016-ij5yaqkx	53	13	20	20	CD
cord-275016-ij5yaqkx	53	14	l	l	NN
cord-275016-ij5yaqkx	53	15	of	of	IN
cord-275016-ij5yaqkx	53	16	the	the	DT
cord-275016-ij5yaqkx	53	17	mixture	mixture	NN
cord-275016-ij5yaqkx	53	18	containing	contain	VBG
cord-275016-ij5yaqkx	53	19	50	50	CD
cord-275016-ij5yaqkx	53	20	mM	mm	CD
cord-275016-ij5yaqkx	53	21	bis	bis	NN
cord-275016-ij5yaqkx	53	22	-	-	HYPH
cord-275016-ij5yaqkx	53	23	trispropane	trispropane	NN
cord-275016-ij5yaqkx	53	24	-	-	HYPH
cord-275016-ij5yaqkx	53	25	HCl	hcl	NN
cord-275016-ij5yaqkx	53	26	buffer	buffer	NN
cord-275016-ij5yaqkx	53	27	(	(	-LRB-
cord-275016-ij5yaqkx	53	28	pH	pH	NNP
cord-275016-ij5yaqkx	53	29	8.62	8.62	CD
cord-275016-ij5yaqkx	53	30	)	)	-RRB-
cord-275016-ij5yaqkx	53	31	for	for	IN
cord-275016-ij5yaqkx	54	1	16	16	CD
cord-275016-ij5yaqkx	54	2	h.	h.	NNP
cord-275016-ij5yaqkx	54	3	Details	detail	NNS
cord-275016-ij5yaqkx	54	4	of	of	IN
cord-275016-ij5yaqkx	54	5	reaction	reaction	NN
cord-275016-ij5yaqkx	54	6	mixtures	mixture	NNS
cord-275016-ij5yaqkx	54	7	were	be	VBD
cord-275016-ij5yaqkx	54	8	described	describe	VBN
cord-275016-ij5yaqkx	54	9	in	in	IN
cord-275016-ij5yaqkx	54	10	the	the	DT
cord-275016-ij5yaqkx	54	11	respective	respective	JJ
cord-275016-ij5yaqkx	54	12	sections	section	NNS
cord-275016-ij5yaqkx	54	13	.	.	.
cord-275016-ij5yaqkx	55	1	The	the	DT
cord-275016-ij5yaqkx	55	2	reaction	reaction	NN
cord-275016-ij5yaqkx	55	3	was	be	VBD
cord-275016-ij5yaqkx	55	4	stopped	stop	VBN
cord-275016-ij5yaqkx	55	5	by	by	IN
cord-275016-ij5yaqkx	55	6	the	the	DT
cord-275016-ij5yaqkx	55	7	addition	addition	NN
cord-275016-ij5yaqkx	55	8	of	of	IN
cord-275016-ij5yaqkx	55	9	an	an	DT
cord-275016-ij5yaqkx	55	10	equal	equal	JJ
cord-275016-ij5yaqkx	55	11	volume	volume	NN
cord-275016-ij5yaqkx	55	12	of	of	IN
cord-275016-ij5yaqkx	55	13	2×	2×	CD
cord-275016-ij5yaqkx	55	14	sample	sample	NN
cord-275016-ij5yaqkx	55	15	buffer	buffer	NN
cord-275016-ij5yaqkx	55	16	,	,	,
cord-275016-ij5yaqkx	55	17	and	and	CC
cord-275016-ij5yaqkx	55	18	the	the	DT
cord-275016-ij5yaqkx	55	19	proteins	protein	NNS
cord-275016-ij5yaqkx	55	20	were	be	VBD
cord-275016-ij5yaqkx	55	21	then	then	RB
cord-275016-ij5yaqkx	55	22	analyzed	analyze	VBN
cord-275016-ij5yaqkx	55	23	by	by	IN
cord-275016-ij5yaqkx	55	24	SDS	sds	NN
cord-275016-ij5yaqkx	55	25	-	-	HYPH
cord-275016-ij5yaqkx	55	26	polyacrylamide	polyacrylamide	NN
cord-275016-ij5yaqkx	55	27	(	(	-LRB-
cord-275016-ij5yaqkx	55	28	13	13	CD
cord-275016-ij5yaqkx	55	29	%	%	NN
cord-275016-ij5yaqkx	55	30	)	)	-RRB-
cord-275016-ij5yaqkx	55	31	gel	gel	NN
cord-275016-ij5yaqkx	55	32	electrophoresis	electrophoresis	NN
cord-275016-ij5yaqkx	55	33	.	.	.
cord-275016-ij5yaqkx	56	1	Gels	gel	NNS
cord-275016-ij5yaqkx	56	2	were	be	VBD
cord-275016-ij5yaqkx	56	3	stained	stain	VBN
cord-275016-ij5yaqkx	56	4	with	with	IN
cord-275016-ij5yaqkx	56	5	Coomassie	Coomassie	NNP
cord-275016-ij5yaqkx	56	6	Brilliant	Brilliant	NNP
cord-275016-ij5yaqkx	56	7	Blue	Blue	NNP
cord-275016-ij5yaqkx	56	8	R-250	r-250	NN
cord-275016-ij5yaqkx	56	9	.	.	.
cord-275016-ij5yaqkx	57	1	In	in	IN
cord-275016-ij5yaqkx	57	2	order	order	NN
cord-275016-ij5yaqkx	57	3	to	to	TO
cord-275016-ij5yaqkx	57	4	estimate	estimate	VB
cord-275016-ij5yaqkx	57	5	the	the	DT
cord-275016-ij5yaqkx	57	6	efficiency	efficiency	NN
cord-275016-ij5yaqkx	57	7	of	of	IN
cord-275016-ij5yaqkx	57	8	the	the	DT
cord-275016-ij5yaqkx	57	9	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	57	10	cleavage	cleavage	NN
cord-275016-ij5yaqkx	57	11	,	,	,
cord-275016-ij5yaqkx	57	12	density	density	NN
cord-275016-ij5yaqkx	57	13	of	of	IN
cord-275016-ij5yaqkx	57	14	the	the	DT
cord-275016-ij5yaqkx	57	15	respective	respective	JJ
cord-275016-ij5yaqkx	57	16	stained	stained	JJ
cord-275016-ij5yaqkx	57	17	bands	band	NNS
cord-275016-ij5yaqkx	57	18	was	be	VBD
cord-275016-ij5yaqkx	57	19	scanned	scan	VBN
cord-275016-ij5yaqkx	57	20	and	and	CC
cord-275016-ij5yaqkx	57	21	calculated	calculate	VBN
cord-275016-ij5yaqkx	57	22	using	use	VBG
cord-275016-ij5yaqkx	57	23	NIH	NIH	NNP
cord-275016-ij5yaqkx	57	24	Image	Image	NNP
cord-275016-ij5yaqkx	57	25	for	for	IN
cord-275016-ij5yaqkx	57	26	Macintosh	Macintosh	NNP
cord-275016-ij5yaqkx	57	27	version	version	NN
cord-275016-ij5yaqkx	57	28	1.62	1.62	CD
cord-275016-ij5yaqkx	57	29	.	.	.
cord-275016-ij5yaqkx	58	1	The	the	DT
cord-275016-ij5yaqkx	58	2	viral	viral	JJ
cord-275016-ij5yaqkx	58	3	non	non	JJ
cord-275016-ij5yaqkx	58	4	-	-	JJ
cord-275016-ij5yaqkx	58	5	structural	structural	JJ
cord-275016-ij5yaqkx	58	6	proteins	protein	NNS
cord-275016-ij5yaqkx	58	7	and	and	CC
cord-275016-ij5yaqkx	58	8	the	the	DT
cord-275016-ij5yaqkx	58	9	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	58	10	cleavage	cleavage	NN
cord-275016-ij5yaqkx	58	11	sites	site	NNS
cord-275016-ij5yaqkx	58	12	within	within	IN
cord-275016-ij5yaqkx	58	13	the	the	DT
cord-275016-ij5yaqkx	58	14	ORF1	ORF1	NNP
cord-275016-ij5yaqkx	58	15	polyprotein	polyprotein	NN
cord-275016-ij5yaqkx	58	16	are	be	VBP
cord-275016-ij5yaqkx	58	17	depicted	depict	VBN
cord-275016-ij5yaqkx	58	18	in	in	IN
cord-275016-ij5yaqkx	58	19	Fig	Fig	NNP
cord-275016-ij5yaqkx	58	20	.	.	.
cord-275016-ij5yaqkx	58	21	1A	1a	NN
cord-275016-ij5yaqkx	58	22	.	.	.
cord-275016-ij5yaqkx	59	1	In	in	IN
cord-275016-ij5yaqkx	59	2	order	order	NN
cord-275016-ij5yaqkx	59	3	to	to	TO
cord-275016-ij5yaqkx	59	4	purify	purify	VB
cord-275016-ij5yaqkx	59	5	the	the	DT
cord-275016-ij5yaqkx	59	6	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	59	7	virus	virus	NN
cord-275016-ij5yaqkx	59	8	3C	3c	NN
cord-275016-ij5yaqkx	59	9	-	-	HYPH
cord-275016-ij5yaqkx	59	10	like	like	JJ
cord-275016-ij5yaqkx	59	11	protease	protease	NN
cord-275016-ij5yaqkx	59	12	,	,	,
cord-275016-ij5yaqkx	59	13	we	-PRON-	PRP
cord-275016-ij5yaqkx	59	14	constructed	construct	VBD
cord-275016-ij5yaqkx	59	15	pT7ProHis	pt7prohis	VBP
cord-275016-ij5yaqkx	59	16	with	with	IN
cord-275016-ij5yaqkx	59	17	the	the	DT
cord-275016-ij5yaqkx	59	18	gene	gene	NN
cord-275016-ij5yaqkx	59	19	fragment	fragment	NN
cord-275016-ij5yaqkx	59	20	encoding	encode	VBG
cord-275016-ij5yaqkx	59	21	the	the	DT
cord-275016-ij5yaqkx	59	22	protease	protease	NN
cord-275016-ij5yaqkx	59	23	with	with	IN
cord-275016-ij5yaqkx	59	24	His	-PRON-	PRP$
cord-275016-ij5yaqkx	59	25	-	-	HYPH
cord-275016-ij5yaqkx	59	26	tag	tag	NN
cord-275016-ij5yaqkx	59	27	in	in	IN
cord-275016-ij5yaqkx	59	28	its	-PRON-	PRP$
cord-275016-ij5yaqkx	59	29	C	c	NN
cord-275016-ij5yaqkx	59	30	-	-	HYPH
cord-275016-ij5yaqkx	59	31	terminus	terminus	NN
cord-275016-ij5yaqkx	59	32	(	(	-LRB-
cord-275016-ij5yaqkx	59	33	Fig	fig	NN
cord-275016-ij5yaqkx	59	34	.	.	.
cord-275016-ij5yaqkx	59	35	1B	1b	CD
cord-275016-ij5yaqkx	59	36	)	)	-RRB-
cord-275016-ij5yaqkx	59	37	.	.	.
cord-275016-ij5yaqkx	60	1	The	the	DT
cord-275016-ij5yaqkx	60	2	ProHis	ProHis	NNP
cord-275016-ij5yaqkx	60	3	protein	protein	NN
cord-275016-ij5yaqkx	60	4	(	(	-LRB-
cord-275016-ij5yaqkx	60	5	calculated	calculate	VBN
cord-275016-ij5yaqkx	60	6	molecular	molecular	JJ
cord-275016-ij5yaqkx	60	7	mass	mass	NN
cord-275016-ij5yaqkx	60	8	of	of	IN
cord-275016-ij5yaqkx	60	9	20.6	20.6	CD
cord-275016-ij5yaqkx	60	10	kDa	kDa	NNPS
cord-275016-ij5yaqkx	60	11	)	)	-RRB-
cord-275016-ij5yaqkx	60	12	expressed	express	VBD
cord-275016-ij5yaqkx	60	13	from	from	IN
cord-275016-ij5yaqkx	60	14	pT7ProHis	pT7ProHis	NNP
cord-275016-ij5yaqkx	60	15	was	be	VBD
cord-275016-ij5yaqkx	60	16	used	use	VBN
cord-275016-ij5yaqkx	60	17	as	as	IN
cord-275016-ij5yaqkx	60	18	an	an	DT
cord-275016-ij5yaqkx	60	19	enzyme	enzyme	NN
cord-275016-ij5yaqkx	60	20	in	in	IN
cord-275016-ij5yaqkx	60	21	this	this	DT
cord-275016-ij5yaqkx	60	22	study	study	NN
cord-275016-ij5yaqkx	60	23	.	.	.
cord-275016-ij5yaqkx	61	1	In	in	IN
cord-275016-ij5yaqkx	61	2	order	order	NN
cord-275016-ij5yaqkx	61	3	to	to	TO
cord-275016-ij5yaqkx	61	4	observe	observe	VB
cord-275016-ij5yaqkx	61	5	proteolysis	proteolysis	NN
cord-275016-ij5yaqkx	61	6	at	at	IN
cord-275016-ij5yaqkx	61	7	the	the	DT
cord-275016-ij5yaqkx	61	8	cleavage	cleavage	NN
cord-275016-ij5yaqkx	61	9	site	site	NN
cord-275016-ij5yaqkx	61	10	between	between	IN
cord-275016-ij5yaqkx	61	11	the	the	DT
cord-275016-ij5yaqkx	61	12	3C	3C	NNP
cord-275016-ij5yaqkx	61	13	and	and	CC
cord-275016-ij5yaqkx	61	14	3D	3D	NNP
cord-275016-ij5yaqkx	61	15	,	,	,
cord-275016-ij5yaqkx	61	16	we	-PRON-	PRP
cord-275016-ij5yaqkx	61	17	at	at	IN
cord-275016-ij5yaqkx	61	18	first	first	RB
cord-275016-ij5yaqkx	61	19	used	use	VBD
cord-275016-ij5yaqkx	61	20	the	the	DT
cord-275016-ij5yaqkx	61	21	His-3CD	His-3CD	NNP
cord-275016-ij5yaqkx	61	22	-	-	HYPH
cord-275016-ij5yaqkx	61	23	C139A	C139A	NNP
cord-275016-ij5yaqkx	61	24	mutant	mutant	JJ
cord-275016-ij5yaqkx	61	25	protein	protein	NN
cord-275016-ij5yaqkx	61	26	expressed	express	VBN
cord-275016-ij5yaqkx	61	27	from	from	IN
cord-275016-ij5yaqkx	61	28	pT7His3CD	pt7his3cd	NN
cord-275016-ij5yaqkx	61	29	-	-	.
cord-275016-ij5yaqkx	61	30	C139A	C139A	NNP
cord-275016-ij5yaqkx	61	31	as	as	IN
cord-275016-ij5yaqkx	61	32	a	a	DT
cord-275016-ij5yaqkx	61	33	substrate	substrate	NN
cord-275016-ij5yaqkx	61	34	,	,	,
cord-275016-ij5yaqkx	61	35	which	which	WDT
cord-275016-ij5yaqkx	61	36	was	be	VBD
cord-275016-ij5yaqkx	61	37	the	the	DT
cord-275016-ij5yaqkx	61	38	N	n	CD
cord-275016-ij5yaqkx	61	39	-	-	HYPH
cord-275016-ij5yaqkx	61	40	terminal	terminal	NN
cord-275016-ij5yaqkx	61	41	His	-PRON-	PRP$
cord-275016-ij5yaqkx	61	42	-	-	HYPH
cord-275016-ij5yaqkx	61	43	tagged	tagged	NNP
cord-275016-ij5yaqkx	61	44	3CD	3CD	NNP
cord-275016-ij5yaqkx	61	45	fragment	fragment	NN
cord-275016-ij5yaqkx	61	46	containing	contain	VBG
cord-275016-ij5yaqkx	61	47	the	the	DT
cord-275016-ij5yaqkx	61	48	Ala	Ala	NNP
cord-275016-ij5yaqkx	61	49	mutation	mutation	NN
cord-275016-ij5yaqkx	61	50	of	of	IN
cord-275016-ij5yaqkx	61	51	active	active	JJ
cord-275016-ij5yaqkx	61	52	-	-	HYPH
cord-275016-ij5yaqkx	61	53	site	site	NN
cord-275016-ij5yaqkx	61	54	Cys139	cys139	CD
cord-275016-ij5yaqkx	61	55	of	of	IN
cord-275016-ij5yaqkx	61	56	the	the	DT
cord-275016-ij5yaqkx	61	57	3C	3c	NN
cord-275016-ij5yaqkx	61	58	-	-	HYPH
cord-275016-ij5yaqkx	61	59	like	like	JJ
cord-275016-ij5yaqkx	61	60	protease	protease	NN
cord-275016-ij5yaqkx	61	61	(	(	-LRB-
cord-275016-ij5yaqkx	61	62	Fig	fig	NN
cord-275016-ij5yaqkx	61	63	.	.	.
cord-275016-ij5yaqkx	61	64	1B	1b	CD
cord-275016-ij5yaqkx	61	65	)	)	-RRB-
cord-275016-ij5yaqkx	61	66	.	.	.
cord-275016-ij5yaqkx	62	1	However	however	RB
cord-275016-ij5yaqkx	62	2	,	,	,
cord-275016-ij5yaqkx	62	3	this	this	DT
cord-275016-ij5yaqkx	62	4	protein	protein	NN
cord-275016-ij5yaqkx	62	5	tended	tend	VBD
cord-275016-ij5yaqkx	62	6	to	to	TO
cord-275016-ij5yaqkx	62	7	aggregate	aggregate	VB
cord-275016-ij5yaqkx	62	8	at	at	IN
cord-275016-ij5yaqkx	62	9	higher	high	JJR
cord-275016-ij5yaqkx	62	10	pH	ph	NN
cord-275016-ij5yaqkx	62	11	after	after	IN
cord-275016-ij5yaqkx	62	12	prolonged	prolong	VBN
cord-275016-ij5yaqkx	62	13	incubation	incubation	NN
cord-275016-ij5yaqkx	62	14	.	.	.
cord-275016-ij5yaqkx	63	1	Other	other	JJ
cord-275016-ij5yaqkx	63	2	parts	part	NNS
cord-275016-ij5yaqkx	63	3	from	from	IN
cord-275016-ij5yaqkx	63	4	the	the	DT
cord-275016-ij5yaqkx	63	5	native	native	JJ
cord-275016-ij5yaqkx	63	6	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	63	7	virus	virus	NN
cord-275016-ij5yaqkx	63	8	ORF1	ORF1	NNP
cord-275016-ij5yaqkx	63	9	polyprotein	polyprotein	NN
cord-275016-ij5yaqkx	63	10	could	could	MD
cord-275016-ij5yaqkx	63	11	not	not	RB
cord-275016-ij5yaqkx	63	12	be	be	VB
cord-275016-ij5yaqkx	63	13	effectively	effectively	RB
cord-275016-ij5yaqkx	63	14	produced	produce	VBN
cord-275016-ij5yaqkx	63	15	in	in	IN
cord-275016-ij5yaqkx	63	16	E.	E.	NNP
cord-275016-ij5yaqkx	63	17	coli	coli	NNS
cord-275016-ij5yaqkx	63	18	cells	cell	NNS
cord-275016-ij5yaqkx	63	19	.	.	.
cord-275016-ij5yaqkx	64	1	Therefore	therefore	RB
cord-275016-ij5yaqkx	64	2	,	,	,
cord-275016-ij5yaqkx	64	3	the	the	DT
cord-275016-ij5yaqkx	64	4	His3Cd	His3Cd	NNP
cord-275016-ij5yaqkx	64	5	-	-	HYPH
cord-275016-ij5yaqkx	64	6	GST	GST	NNP
cord-275016-ij5yaqkx	64	7	protein	protein	NN
cord-275016-ij5yaqkx	64	8	(	(	-LRB-
cord-275016-ij5yaqkx	64	9	calculated	calculate	VBN
cord-275016-ij5yaqkx	64	10	molecular	molecular	JJ
cord-275016-ij5yaqkx	64	11	mass	mass	NN
cord-275016-ij5yaqkx	64	12	of	of	IN
cord-275016-ij5yaqkx	64	13	48.0	48.0	CD
cord-275016-ij5yaqkx	64	14	kDa	kDa	NNP
cord-275016-ij5yaqkx	64	15	)	)	-RRB-
cord-275016-ij5yaqkx	64	16	was	be	VBD
cord-275016-ij5yaqkx	64	17	used	use	VBN
cord-275016-ij5yaqkx	64	18	as	as	IN
cord-275016-ij5yaqkx	64	19	a	a	DT
cord-275016-ij5yaqkx	64	20	substrate	substrate	NN
cord-275016-ij5yaqkx	64	21	,	,	,
cord-275016-ij5yaqkx	64	22	which	which	WDT
cord-275016-ij5yaqkx	64	23	retains	retain	VBZ
cord-275016-ij5yaqkx	64	24	the	the	DT
cord-275016-ij5yaqkx	64	25	cleavage	cleavage	NN
cord-275016-ij5yaqkx	64	26	site	site	NN
cord-275016-ij5yaqkx	64	27	between	between	IN
cord-275016-ij5yaqkx	64	28	the	the	DT
cord-275016-ij5yaqkx	64	29	3C	3c	NN
cord-275016-ij5yaqkx	64	30	and	and	CC
cord-275016-ij5yaqkx	64	31	3D.	3D.	NNP
cord-275016-ij5yaqkx	65	1	The	the	DT
cord-275016-ij5yaqkx	65	2	region	region	NN
cord-275016-ij5yaqkx	65	3	covering	cover	VBG
cord-275016-ij5yaqkx	65	4	the	the	DT
cord-275016-ij5yaqkx	65	5	entire	entire	JJ
cord-275016-ij5yaqkx	65	6	3C	3c	NN
cord-275016-ij5yaqkx	65	7	and	and	CC
cord-275016-ij5yaqkx	65	8	the	the	DT
cord-275016-ij5yaqkx	65	9	N	N	NNP
cord-275016-ij5yaqkx	65	10	-	-	HYPH
cord-275016-ij5yaqkx	65	11	terminal	terminal	JJ
cord-275016-ij5yaqkx	65	12	19	19	CD
cord-275016-ij5yaqkx	65	13	amino	amino	JJ
cord-275016-ij5yaqkx	65	14	acids	acid	NNS
cord-275016-ij5yaqkx	65	15	of	of	IN
cord-275016-ij5yaqkx	65	16	3D	3D	NNP
cord-275016-ij5yaqkx	65	17	was	be	VBD
cord-275016-ij5yaqkx	65	18	placed	place	VBN
cord-275016-ij5yaqkx	65	19	under	under	IN
cord-275016-ij5yaqkx	65	20	the	the	DT
cord-275016-ij5yaqkx	65	21	control	control	NN
cord-275016-ij5yaqkx	65	22	of	of	IN
cord-275016-ij5yaqkx	65	23	T7	T7	NNP
cord-275016-ij5yaqkx	65	24	promoter	promoter	NN
cord-275016-ij5yaqkx	65	25	with	with	IN
cord-275016-ij5yaqkx	65	26	the	the	DT
cord-275016-ij5yaqkx	65	27	N	n	CD
cord-275016-ij5yaqkx	65	28	-	-	HYPH
cord-275016-ij5yaqkx	65	29	terminal	terminal	NN
cord-275016-ij5yaqkx	65	30	His	-PRON-	PRP$
cord-275016-ij5yaqkx	65	31	-	-	HYPH
cord-275016-ij5yaqkx	65	32	tag	tag	NN
cord-275016-ij5yaqkx	65	33	and	and	CC
cord-275016-ij5yaqkx	65	34	the	the	DT
cord-275016-ij5yaqkx	65	35	C	c	NN
cord-275016-ij5yaqkx	65	36	-	-	HYPH
cord-275016-ij5yaqkx	65	37	terminal	terminal	NN
cord-275016-ij5yaqkx	65	38	glutathione	glutathione	NN
cord-275016-ij5yaqkx	65	39	S	s	NN
cord-275016-ij5yaqkx	65	40	-	-	HYPH
cord-275016-ij5yaqkx	65	41	transferase	transferase	NN
cord-275016-ij5yaqkx	65	42	(	(	-LRB-
cord-275016-ij5yaqkx	65	43	GST	GST	NNP
cord-275016-ij5yaqkx	65	44	)	)	-RRB-
cord-275016-ij5yaqkx	65	45	,	,	,
cord-275016-ij5yaqkx	65	46	the	the	DT
cord-275016-ij5yaqkx	65	47	resultant	resultant	JJ
cord-275016-ij5yaqkx	65	48	plasmid	plasmid	NN
cord-275016-ij5yaqkx	65	49	being	be	VBG
cord-275016-ij5yaqkx	65	50	designated	designate	VBN
cord-275016-ij5yaqkx	65	51	pT7His3Cd	pT7His3Cd	NNP
cord-275016-ij5yaqkx	65	52	-	-	HYPH
cord-275016-ij5yaqkx	65	53	GST	gst	NN
cord-275016-ij5yaqkx	65	54	(	(	-LRB-
cord-275016-ij5yaqkx	65	55	Fig	fig	NN
cord-275016-ij5yaqkx	65	56	.	.	.
cord-275016-ij5yaqkx	65	57	1B	1b	CD
cord-275016-ij5yaqkx	65	58	)	)	-RRB-
cord-275016-ij5yaqkx	65	59	.	.	.
cord-275016-ij5yaqkx	66	1	Although	although	IN
cord-275016-ij5yaqkx	66	2	this	this	DT
cord-275016-ij5yaqkx	66	3	protein	protein	NN
cord-275016-ij5yaqkx	66	4	itself	-PRON-	PRP
cord-275016-ij5yaqkx	66	5	has	have	VBZ
cord-275016-ij5yaqkx	66	6	the	the	DT
cord-275016-ij5yaqkx	66	7	3C	3c	NN
cord-275016-ij5yaqkx	66	8	-	-	HYPH
cord-275016-ij5yaqkx	66	9	like	like	JJ
cord-275016-ij5yaqkx	66	10	protease	protease	NN
cord-275016-ij5yaqkx	66	11	moiety	moiety	NN
cord-275016-ij5yaqkx	66	12	,	,	,
cord-275016-ij5yaqkx	66	13	it	-PRON-	PRP
cord-275016-ij5yaqkx	66	14	never	never	RB
cord-275016-ij5yaqkx	66	15	undergoes	undergo	VBZ
cord-275016-ij5yaqkx	66	16	autocatalytic	autocatalytic	JJ
cord-275016-ij5yaqkx	66	17	cleavage	cleavage	NN
cord-275016-ij5yaqkx	66	18	because	because	IN
cord-275016-ij5yaqkx	66	19	the	the	DT
cord-275016-ij5yaqkx	66	20	C139A	C139A	NNP
cord-275016-ij5yaqkx	66	21	mutation	mutation	NN
cord-275016-ij5yaqkx	66	22	is	be	VBZ
cord-275016-ij5yaqkx	66	23	introduced	introduce	VBN
cord-275016-ij5yaqkx	66	24	.	.	.
cord-275016-ij5yaqkx	67	1	If	if	IN
cord-275016-ij5yaqkx	67	2	His3Cd	His3Cd	NNP
cord-275016-ij5yaqkx	67	3	-	-	HYPH
cord-275016-ij5yaqkx	67	4	GST	GST	NNP
cord-275016-ij5yaqkx	67	5	is	be	VBZ
cord-275016-ij5yaqkx	67	6	cleaved	cleave	VBN
cord-275016-ij5yaqkx	67	7	at	at	IN
cord-275016-ij5yaqkx	67	8	the	the	DT
cord-275016-ij5yaqkx	67	9	3C/3D	3c/3d	CD
cord-275016-ij5yaqkx	67	10	junction	junction	NN
cord-275016-ij5yaqkx	67	11	(	(	-LRB-
cord-275016-ij5yaqkx	67	12	LE	LE	NNP
cord-275016-ij5yaqkx	67	13	/	/	SYM
cord-275016-ij5yaqkx	67	14	GG	GG	NNP
cord-275016-ij5yaqkx	67	15	)	)	-RRB-
cord-275016-ij5yaqkx	67	16	by	by	IN
cord-275016-ij5yaqkx	67	17	the	the	DT
cord-275016-ij5yaqkx	67	18	active	active	JJ
cord-275016-ij5yaqkx	67	19	ProHis	ProHis	NNP
cord-275016-ij5yaqkx	67	20	,	,	,
cord-275016-ij5yaqkx	67	21	the	the	DT
cord-275016-ij5yaqkx	67	22	His3C	his3c	NN
cord-275016-ij5yaqkx	67	23	-	-	.
cord-275016-ij5yaqkx	67	24	C139A	C139A	NNP
cord-275016-ij5yaqkx	67	25	moiety	moiety	NN
cord-275016-ij5yaqkx	67	26	of	of	IN
cord-275016-ij5yaqkx	67	27	20.7	20.7	CD
cord-275016-ij5yaqkx	67	28	kDa	kDa	NNPS
cord-275016-ij5yaqkx	67	29	and	and	CC
cord-275016-ij5yaqkx	67	30	the	the	DT
cord-275016-ij5yaqkx	67	31	3D	3D	NNP
cord-275016-ij5yaqkx	67	32	-GST	-GST	-LRB-
cord-275016-ij5yaqkx	67	33	moiety	moiety	NN
cord-275016-ij5yaqkx	67	34	of	of	IN
cord-275016-ij5yaqkx	67	35	27.3	27.3	CD
cord-275016-ij5yaqkx	67	36	kDa	kDa	NNS
cord-275016-ij5yaqkx	67	37	are	be	VBP
cord-275016-ij5yaqkx	67	38	produced	produce	VBN
cord-275016-ij5yaqkx	67	39	.	.	.
cord-275016-ij5yaqkx	68	1	Enzymatic	enzymatic	JJ
cord-275016-ij5yaqkx	68	2	reaction	reaction	NN
cord-275016-ij5yaqkx	68	3	was	be	VBD
cord-275016-ij5yaqkx	68	4	initiated	initiate	VBN
cord-275016-ij5yaqkx	68	5	by	by	IN
cord-275016-ij5yaqkx	68	6	mixing	mix	VBG
cord-275016-ij5yaqkx	68	7	enzyme	enzyme	NN
cord-275016-ij5yaqkx	68	8	(	(	-LRB-
cord-275016-ij5yaqkx	68	9	ProHis	prohis	NN
cord-275016-ij5yaqkx	68	10	)	)	-RRB-
cord-275016-ij5yaqkx	68	11	and	and	CC
cord-275016-ij5yaqkx	68	12	substrate	substrate	NN
cord-275016-ij5yaqkx	68	13	(	(	-LRB-
cord-275016-ij5yaqkx	68	14	His3Cd	His3Cd	NNP
cord-275016-ij5yaqkx	68	15	-	-	HYPH
cord-275016-ij5yaqkx	68	16	GST	GST	NNP
cord-275016-ij5yaqkx	68	17	)	)	-RRB-
cord-275016-ij5yaqkx	68	18	.	.	.
cord-275016-ij5yaqkx	69	1	We	-PRON-	PRP
cord-275016-ij5yaqkx	69	2	could	could	MD
cord-275016-ij5yaqkx	69	3	not	not	RB
cord-275016-ij5yaqkx	69	4	obtain	obtain	VB
cord-275016-ij5yaqkx	69	5	clear	clear	JJ
cord-275016-ij5yaqkx	69	6	results	result	NNS
cord-275016-ij5yaqkx	69	7	when	when	WRB
cord-275016-ij5yaqkx	69	8	the	the	DT
cord-275016-ij5yaqkx	69	9	reaction	reaction	NN
cord-275016-ij5yaqkx	69	10	was	be	VBD
cord-275016-ij5yaqkx	69	11	performed	perform	VBN
cord-275016-ij5yaqkx	69	12	in	in	IN
cord-275016-ij5yaqkx	69	13	a	a	DT
cord-275016-ij5yaqkx	69	14	short	short	JJ
cord-275016-ij5yaqkx	69	15	period	period	NN
cord-275016-ij5yaqkx	69	16	(	(	-LRB-
cord-275016-ij5yaqkx	69	17	2	2	CD
cord-275016-ij5yaqkx	69	18	-	-	SYM
cord-275016-ij5yaqkx	69	19	4	4	CD
cord-275016-ij5yaqkx	69	20	h	h	NN
cord-275016-ij5yaqkx	69	21	)	)	-RRB-
cord-275016-ij5yaqkx	69	22	(	(	-LRB-
cord-275016-ij5yaqkx	69	23	data	datum	NNS
cord-275016-ij5yaqkx	69	24	not	not	RB
cord-275016-ij5yaqkx	69	25	shown	show	VBN
cord-275016-ij5yaqkx	69	26	)	)	-RRB-
cord-275016-ij5yaqkx	69	27	.	.	.
cord-275016-ij5yaqkx	70	1	However	however	RB
cord-275016-ij5yaqkx	70	2	,	,	,
cord-275016-ij5yaqkx	70	3	even	even	RB
cord-275016-ij5yaqkx	70	4	if	if	IN
cord-275016-ij5yaqkx	70	5	a	a	DT
cord-275016-ij5yaqkx	70	6	substrate	substrate	NN
cord-275016-ij5yaqkx	70	7	was	be	VBD
cord-275016-ij5yaqkx	70	8	mixed	mix	VBN
cord-275016-ij5yaqkx	70	9	with	with	IN
cord-275016-ij5yaqkx	70	10	an	an	DT
cord-275016-ij5yaqkx	70	11	enzyme	enzyme	NN
cord-275016-ij5yaqkx	70	12	for	for	IN
cord-275016-ij5yaqkx	70	13	a	a	DT
cord-275016-ij5yaqkx	70	14	long	long	JJ
cord-275016-ij5yaqkx	70	15	period	period	NN
cord-275016-ij5yaqkx	70	16	(	(	-LRB-
cord-275016-ij5yaqkx	70	17	more	more	JJR
cord-275016-ij5yaqkx	70	18	than	than	IN
cord-275016-ij5yaqkx	70	19	24	24	CD
cord-275016-ij5yaqkx	70	20	h	h	NNP
cord-275016-ij5yaqkx	70	21	)	)	-RRB-
cord-275016-ij5yaqkx	70	22	,	,	,
cord-275016-ij5yaqkx	70	23	complete	complete	JJ
cord-275016-ij5yaqkx	70	24	cleavage	cleavage	NN
cord-275016-ij5yaqkx	70	25	could	could	MD
cord-275016-ij5yaqkx	70	26	not	not	RB
cord-275016-ij5yaqkx	70	27	be	be	VB
cord-275016-ij5yaqkx	70	28	achieved	achieve	VBN
cord-275016-ij5yaqkx	70	29	.	.	.
cord-275016-ij5yaqkx	71	1	One	one	CD
cord-275016-ij5yaqkx	71	2	possibility	possibility	NN
cord-275016-ij5yaqkx	71	3	for	for	IN
cord-275016-ij5yaqkx	71	4	the	the	DT
cord-275016-ij5yaqkx	71	5	incomplete	incomplete	JJ
cord-275016-ij5yaqkx	71	6	cleavage	cleavage	NN
cord-275016-ij5yaqkx	71	7	is	be	VBZ
cord-275016-ij5yaqkx	71	8	that	that	IN
cord-275016-ij5yaqkx	71	9	the	the	DT
cord-275016-ij5yaqkx	71	10	cleavage	cleavage	NN
cord-275016-ij5yaqkx	71	11	site	site	NN
cord-275016-ij5yaqkx	71	12	of	of	IN
cord-275016-ij5yaqkx	71	13	the	the	DT
cord-275016-ij5yaqkx	71	14	substrate	substrate	NN
cord-275016-ij5yaqkx	71	15	(	(	-LRB-
cord-275016-ij5yaqkx	71	16	His3Cd	His3Cd	NNP
cord-275016-ij5yaqkx	71	17	-	-	HYPH
cord-275016-ij5yaqkx	71	18	GST	GST	NNP
cord-275016-ij5yaqkx	71	19	)	)	-RRB-
cord-275016-ij5yaqkx	71	20	could	could	MD
cord-275016-ij5yaqkx	71	21	not	not	RB
cord-275016-ij5yaqkx	71	22	be	be	VB
cord-275016-ij5yaqkx	71	23	accessible	accessible	JJ
cord-275016-ij5yaqkx	71	24	by	by	IN
cord-275016-ij5yaqkx	71	25	the	the	DT
cord-275016-ij5yaqkx	71	26	protease	protease	NN
cord-275016-ij5yaqkx	71	27	because	because	IN
cord-275016-ij5yaqkx	71	28	the	the	DT
cord-275016-ij5yaqkx	71	29	substrate	substrate	NN
cord-275016-ij5yaqkx	71	30	was	be	VBD
cord-275016-ij5yaqkx	71	31	not	not	RB
cord-275016-ij5yaqkx	71	32	properly	properly	RB
cord-275016-ij5yaqkx	71	33	folded	fold	VBN
cord-275016-ij5yaqkx	71	34	.	.	.
cord-275016-ij5yaqkx	72	1	Alternatively	alternatively	RB
cord-275016-ij5yaqkx	72	2	,	,	,
cord-275016-ij5yaqkx	72	3	part	part	NN
cord-275016-ij5yaqkx	72	4	of	of	IN
cord-275016-ij5yaqkx	72	5	the	the	DT
cord-275016-ij5yaqkx	72	6	cleavage	cleavage	NN
cord-275016-ij5yaqkx	72	7	site	site	NN
cord-275016-ij5yaqkx	72	8	in	in	IN
cord-275016-ij5yaqkx	72	9	the	the	DT
cord-275016-ij5yaqkx	72	10	substrate	substrate	NN
cord-275016-ij5yaqkx	72	11	might	may	MD
cord-275016-ij5yaqkx	72	12	be	be	VB
cord-275016-ij5yaqkx	72	13	masked	mask	VBN
cord-275016-ij5yaqkx	72	14	by	by	IN
cord-275016-ij5yaqkx	72	15	its	-PRON-	PRP$
cord-275016-ij5yaqkx	72	16	own	own	JJ
cord-275016-ij5yaqkx	72	17	catalytic	catalytic	JJ
cord-275016-ij5yaqkx	72	18	site	site	NN
cord-275016-ij5yaqkx	72	19	or	or	CC
cord-275016-ij5yaqkx	72	20	the	the	DT
cord-275016-ij5yaqkx	72	21	catalytic	catalytic	JJ
cord-275016-ij5yaqkx	72	22	site	site	NN
cord-275016-ij5yaqkx	72	23	of	of	IN
cord-275016-ij5yaqkx	72	24	other	other	JJ
cord-275016-ij5yaqkx	72	25	substrate	substrate	NN
cord-275016-ij5yaqkx	72	26	molecule	molecule	NN
cord-275016-ij5yaqkx	72	27	,	,	,
cord-275016-ij5yaqkx	72	28	so	so	IN
cord-275016-ij5yaqkx	72	29	that	that	IN
cord-275016-ij5yaqkx	72	30	the	the	DT
cord-275016-ij5yaqkx	72	31	enzyme	enzyme	NN
cord-275016-ij5yaqkx	72	32	might	may	MD
cord-275016-ij5yaqkx	72	33	have	have	VB
cord-275016-ij5yaqkx	72	34	competed	compete	VBN
cord-275016-ij5yaqkx	72	35	with	with	IN
cord-275016-ij5yaqkx	72	36	the	the	DT
cord-275016-ij5yaqkx	72	37	substrate	substrate	NN
cord-275016-ij5yaqkx	72	38	for	for	IN
cord-275016-ij5yaqkx	72	39	the	the	DT
cord-275016-ij5yaqkx	72	40	cleavage	cleavage	NN
cord-275016-ij5yaqkx	72	41	site	site	NN
cord-275016-ij5yaqkx	72	42	.	.	.
cord-275016-ij5yaqkx	73	1	Very	very	RB
cord-275016-ij5yaqkx	73	2	recently	recently	RB
cord-275016-ij5yaqkx	73	3	,	,	,
cord-275016-ij5yaqkx	73	4	Belliot	Belliot	NNP
cord-275016-ij5yaqkx	73	5	et	et	NNP
cord-275016-ij5yaqkx	73	6	al	al	NNP
cord-275016-ij5yaqkx	73	7	.	.	.
cord-275016-ij5yaqkx	74	1	(	(	-LRB-
cord-275016-ij5yaqkx	74	2	2003	2003	CD
cord-275016-ij5yaqkx	74	3	)	)	-RRB-
cord-275016-ij5yaqkx	74	4	reported	report	VBD
cord-275016-ij5yaqkx	74	5	trans	trans	JJ
cord-275016-ij5yaqkx	74	6	cleavage	cleavage	NN
cord-275016-ij5yaqkx	74	7	activity	activity	NN
cord-275016-ij5yaqkx	74	8	of	of	IN
cord-275016-ij5yaqkx	74	9	the	the	DT
cord-275016-ij5yaqkx	74	10	3C	3c	NN
cord-275016-ij5yaqkx	74	11	-	-	HYPH
cord-275016-ij5yaqkx	74	12	like	like	JJ
cord-275016-ij5yaqkx	74	13	protease	protease	NN
cord-275016-ij5yaqkx	74	14	from	from	IN
cord-275016-ij5yaqkx	74	15	the	the	DT
cord-275016-ij5yaqkx	74	16	genogroup	genogroup	NN
cord-275016-ij5yaqkx	74	17	II	II	NNP
cord-275016-ij5yaqkx	74	18	MD145	MD145	NNP
cord-275016-ij5yaqkx	74	19	Norovirus	norovirus	NN
cord-275016-ij5yaqkx	74	20	(	(	-LRB-
cord-275016-ij5yaqkx	74	21	Belliot	Belliot	NNP
cord-275016-ij5yaqkx	74	22	et	et	FW
cord-275016-ij5yaqkx	74	23	al	al	NNP
cord-275016-ij5yaqkx	74	24	.	.	NNP
cord-275016-ij5yaqkx	74	25	,	,	,
cord-275016-ij5yaqkx	74	26	2003	2003	CD
cord-275016-ij5yaqkx	74	27	)	)	-RRB-
cord-275016-ij5yaqkx	74	28	.	.	.
cord-275016-ij5yaqkx	75	1	They	-PRON-	PRP
cord-275016-ij5yaqkx	75	2	used	use	VBD
cord-275016-ij5yaqkx	75	3	bacterially	bacterially	RB
cord-275016-ij5yaqkx	75	4	expressed	express	VBN
cord-275016-ij5yaqkx	75	5	3C	3c	NN
cord-275016-ij5yaqkx	75	6	-	-	HYPH
cord-275016-ij5yaqkx	75	7	like	like	JJ
cord-275016-ij5yaqkx	75	8	protease	protease	NN
cord-275016-ij5yaqkx	75	9	with	with	IN
cord-275016-ij5yaqkx	75	10	its	-PRON-	PRP$
cord-275016-ij5yaqkx	75	11	C	c	NN
cord-275016-ij5yaqkx	75	12	-	-	HYPH
cord-275016-ij5yaqkx	75	13	terminus	terminus	NN
cord-275016-ij5yaqkx	75	14	His	-PRON-	PRP$
cord-275016-ij5yaqkx	75	15	-	-	HYPH
cord-275016-ij5yaqkx	75	16	tagged	tagged	NNP
cord-275016-ij5yaqkx	75	17	as	as	IN
cord-275016-ij5yaqkx	75	18	an	an	DT
cord-275016-ij5yaqkx	75	19	enzyme	enzyme	NN
cord-275016-ij5yaqkx	75	20	and	and	CC
cord-275016-ij5yaqkx	75	21	the	the	DT
cord-275016-ij5yaqkx	75	22	entire	entire	JJ
cord-275016-ij5yaqkx	75	23	ORF1	ORF1	NNP
cord-275016-ij5yaqkx	75	24	protein	protein	NN
cord-275016-ij5yaqkx	75	25	or	or	CC
cord-275016-ij5yaqkx	75	26	3CD	3cd	CD
cord-275016-ij5yaqkx	75	27	fragment	fragment	NN
cord-275016-ij5yaqkx	75	28	containing	contain	VBG
cord-275016-ij5yaqkx	75	29	the	the	DT
cord-275016-ij5yaqkx	75	30	active	active	JJ
cord-275016-ij5yaqkx	75	31	-	-	HYPH
cord-275016-ij5yaqkx	75	32	site	site	NN
cord-275016-ij5yaqkx	75	33	Cys	Cys	NNP
cord-275016-ij5yaqkx	75	34	mutation	mutation	NN
cord-275016-ij5yaqkx	75	35	as	as	IN
cord-275016-ij5yaqkx	75	36	a	a	DT
cord-275016-ij5yaqkx	75	37	substrate	substrate	NN
cord-275016-ij5yaqkx	75	38	which	which	WDT
cord-275016-ij5yaqkx	75	39	was	be	VBD
cord-275016-ij5yaqkx	75	40	expressed	express	VBN
cord-275016-ij5yaqkx	75	41	in	in	IN
cord-275016-ij5yaqkx	75	42	the	the	DT
cord-275016-ij5yaqkx	75	43	in	in	FW
cord-275016-ij5yaqkx	75	44	vitro	vitro	FW
cord-275016-ij5yaqkx	75	45	transcription	transcription	NN
cord-275016-ij5yaqkx	75	46	/	/	SYM
cord-275016-ij5yaqkx	75	47	translation	translation	NN
cord-275016-ij5yaqkx	75	48	reaction	reaction	NN
cord-275016-ij5yaqkx	75	49	.	.	.
cord-275016-ij5yaqkx	76	1	Cleavage	cleavage	NN
cord-275016-ij5yaqkx	76	2	reaction	reaction	NN
cord-275016-ij5yaqkx	76	3	was	be	VBD
cord-275016-ij5yaqkx	76	4	performed	perform	VBN
cord-275016-ij5yaqkx	76	5	in	in	IN
cord-275016-ij5yaqkx	76	6	the	the	DT
cord-275016-ij5yaqkx	76	7	pH	pH	NNP
cord-275016-ij5yaqkx	76	8	7.4	7.4	CD
cord-275016-ij5yaqkx	76	9	buffer	buffer	NN
cord-275016-ij5yaqkx	76	10	at	at	IN
cord-275016-ij5yaqkx	76	11	30	30	CD
cord-275016-ij5yaqkx	76	12	•	•	NNP
cord-275016-ij5yaqkx	76	13	C.	C.	NNP
cord-275016-ij5yaqkx	76	14	Data	Data	NNP
cord-275016-ij5yaqkx	76	15	showed	show	VBD
cord-275016-ij5yaqkx	76	16	that	that	IN
cord-275016-ij5yaqkx	76	17	the	the	DT
cord-275016-ij5yaqkx	76	18	3C	3c	NN
cord-275016-ij5yaqkx	76	19	-	-	HYPH
cord-275016-ij5yaqkx	76	20	like	like	JJ
cord-275016-ij5yaqkx	76	21	protease	protease	NN
cord-275016-ij5yaqkx	76	22	cleaved	cleave	VBN
cord-275016-ij5yaqkx	76	23	not	not	RB
cord-275016-ij5yaqkx	76	24	only	only	RB
cord-275016-ij5yaqkx	76	25	the	the	DT
cord-275016-ij5yaqkx	76	26	3C/3D	3c/3d	CD
cord-275016-ij5yaqkx	76	27	junction	junction	NN
cord-275016-ij5yaqkx	76	28	but	but	CC
cord-275016-ij5yaqkx	76	29	also	also	RB
cord-275016-ij5yaqkx	76	30	other	other	JJ
cord-275016-ij5yaqkx	76	31	proposed	propose	VBN
cord-275016-ij5yaqkx	76	32	junctions	junction	NNS
cord-275016-ij5yaqkx	76	33	,	,	,
cord-275016-ij5yaqkx	76	34	and	and	CC
cord-275016-ij5yaqkx	77	1	that	that	DT
cord-275016-ij5yaqkx	77	2	substrate	substrate	NN
cord-275016-ij5yaqkx	77	3	protein	protein	NN
cord-275016-ij5yaqkx	77	4	appeared	appear	VBD
cord-275016-ij5yaqkx	77	5	to	to	TO
cord-275016-ij5yaqkx	77	6	be	be	VB
cord-275016-ij5yaqkx	77	7	cleaved	cleave	VBN
cord-275016-ij5yaqkx	77	8	completely	completely	RB
cord-275016-ij5yaqkx	77	9	after	after	IN
cord-275016-ij5yaqkx	77	10	24-h	24-h	JJ
cord-275016-ij5yaqkx	77	11	incubation	incubation	NN
cord-275016-ij5yaqkx	77	12	.	.	.
cord-275016-ij5yaqkx	78	1	However	however	RB
cord-275016-ij5yaqkx	78	2	,	,	,
cord-275016-ij5yaqkx	78	3	consistent	consistent	JJ
cord-275016-ij5yaqkx	78	4	with	with	IN
cord-275016-ij5yaqkx	78	5	our	-PRON-	PRP$
cord-275016-ij5yaqkx	78	6	experiment	experiment	NN
cord-275016-ij5yaqkx	78	7	,	,	,
cord-275016-ij5yaqkx	78	8	a	a	DT
cord-275016-ij5yaqkx	78	9	long	long	JJ
cord-275016-ij5yaqkx	78	10	time	time	NN
cord-275016-ij5yaqkx	78	11	incubation	incubation	NN
cord-275016-ij5yaqkx	78	12	was	be	VBD
cord-275016-ij5yaqkx	78	13	required	require	VBN
cord-275016-ij5yaqkx	78	14	for	for	IN
cord-275016-ij5yaqkx	78	15	the	the	DT
cord-275016-ij5yaqkx	78	16	result	result	NN
cord-275016-ij5yaqkx	78	17	being	be	VBG
cord-275016-ij5yaqkx	78	18	obvious	obvious	JJ
cord-275016-ij5yaqkx	78	19	.	.	.
cord-275016-ij5yaqkx	79	1	Five	five	CD
cord-275016-ij5yaqkx	79	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	79	3	enzyme	enzyme	NN
cord-275016-ij5yaqkx	79	4	and	and	CC
cord-275016-ij5yaqkx	79	5	5	5	CD
cord-275016-ij5yaqkx	79	6	M	m	NN
cord-275016-ij5yaqkx	79	7	substrate	substrate	NN
cord-275016-ij5yaqkx	79	8	were	be	VBD
cord-275016-ij5yaqkx	79	9	mixed	mix	VBN
cord-275016-ij5yaqkx	79	10	in	in	IN
cord-275016-ij5yaqkx	79	11	50	50	CD
cord-275016-ij5yaqkx	79	12	mM	mm	CD
cord-275016-ij5yaqkx	79	13	bis	bis	NN
cord-275016-ij5yaqkx	79	14	-	-	HYPH
cord-275016-ij5yaqkx	79	15	tris	tris	JJ
cord-275016-ij5yaqkx	79	16	-	-	HYPH
cord-275016-ij5yaqkx	79	17	propane	propane	NN
cord-275016-ij5yaqkx	79	18	-	-	HYPH
cord-275016-ij5yaqkx	79	19	HCl	hcl	NN
cord-275016-ij5yaqkx	79	20	buffer	buffer	NN
cord-275016-ij5yaqkx	79	21	of	of	IN
cord-275016-ij5yaqkx	79	22	various	various	JJ
cord-275016-ij5yaqkx	79	23	pH	ph	NN
cord-275016-ij5yaqkx	79	24	containing	contain	VBG
cord-275016-ij5yaqkx	79	25	25	25	CD
cord-275016-ij5yaqkx	79	26	mM	mm	CD
cord-275016-ij5yaqkx	79	27	NaCl	nacl	NN
cord-275016-ij5yaqkx	79	28	,	,	,
cord-275016-ij5yaqkx	79	29	and	and	CC
cord-275016-ij5yaqkx	79	30	reacted	react	VBD
cord-275016-ij5yaqkx	79	31	at	at	IN
cord-275016-ij5yaqkx	79	32	37	37	CD
cord-275016-ij5yaqkx	79	33	•	•	NN
cord-275016-ij5yaqkx	79	34	C	C	NNP
cord-275016-ij5yaqkx	79	35	for	for	IN
cord-275016-ij5yaqkx	79	36	16	16	CD
cord-275016-ij5yaqkx	79	37	h.	h.	NNP
cord-275016-ij5yaqkx	79	38	Fig	Fig	NNP
cord-275016-ij5yaqkx	79	39	.	.	.
cord-275016-ij5yaqkx	80	1	2	2	LS
cord-275016-ij5yaqkx	80	2	shows	show	VBZ
cord-275016-ij5yaqkx	80	3	that	that	IN
cord-275016-ij5yaqkx	80	4	maximum	maximum	JJ
cord-275016-ij5yaqkx	80	5	cleavage	cleavage	NN
cord-275016-ij5yaqkx	80	6	was	be	VBD
cord-275016-ij5yaqkx	80	7	obtained	obtain	VBN
cord-275016-ij5yaqkx	80	8	at	at	IN
cord-275016-ij5yaqkx	80	9	pH	pH	NNP
cord-275016-ij5yaqkx	80	10	8.62	8.62	CD
cord-275016-ij5yaqkx	80	11	,	,	,
cord-275016-ij5yaqkx	81	1	although	although	IN
cord-275016-ij5yaqkx	81	2	the	the	DT
cord-275016-ij5yaqkx	81	3	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	81	4	cleavage	cleavage	NN
cord-275016-ij5yaqkx	81	5	occurred	occur	VBD
cord-275016-ij5yaqkx	81	6	over	over	IN
cord-275016-ij5yaqkx	81	7	a	a	DT
cord-275016-ij5yaqkx	81	8	wide	wide	JJ
cord-275016-ij5yaqkx	81	9	range	range	NN
cord-275016-ij5yaqkx	81	10	of	of	IN
cord-275016-ij5yaqkx	81	11	pH.	ph.	NN
cord-275016-ij5yaqkx	81	12	Faint	Faint	NNP
cord-275016-ij5yaqkx	81	13	bands	band	NNS
cord-275016-ij5yaqkx	81	14	of	of	IN
cord-275016-ij5yaqkx	81	15	80	80	CD
cord-275016-ij5yaqkx	81	16	and	and	CC
cord-275016-ij5yaqkx	81	17	60	60	CD
cord-275016-ij5yaqkx	81	18	kDa	kDa	NNS
cord-275016-ij5yaqkx	81	19	were	be	VBD
cord-275016-ij5yaqkx	81	20	thought	think	VBN
cord-275016-ij5yaqkx	81	21	to	to	TO
cord-275016-ij5yaqkx	81	22	be	be	VB
cord-275016-ij5yaqkx	81	23	dimers	dimer	NNS
cord-275016-ij5yaqkx	81	24	of	of	IN
cord-275016-ij5yaqkx	81	25	the	the	DT
cord-275016-ij5yaqkx	81	26	substrate	substrate	NN
cord-275016-ij5yaqkx	81	27	and	and	CC
cord-275016-ij5yaqkx	81	28	its	-PRON-	PRP$
cord-275016-ij5yaqkx	81	29	cleaved	cleaved	JJ
cord-275016-ij5yaqkx	81	30	product	product	NN
cord-275016-ij5yaqkx	81	31	(	(	-LRB-
cord-275016-ij5yaqkx	81	32	3D	3D	NNP
cord-275016-ij5yaqkx	81	33	-GST	-GST	-RRB-
cord-275016-ij5yaqkx	81	34	)	)	-RRB-
cord-275016-ij5yaqkx	81	35	,	,	,
cord-275016-ij5yaqkx	81	36	respectively	respectively	RB
cord-275016-ij5yaqkx	81	37	.	.	.
cord-275016-ij5yaqkx	82	1	When	when	WRB
cord-275016-ij5yaqkx	82	2	the	the	DT
cord-275016-ij5yaqkx	82	3	efficiency	efficiency	NN
cord-275016-ij5yaqkx	82	4	of	of	IN
cord-275016-ij5yaqkx	82	5	cleavage	cleavage	NN
cord-275016-ij5yaqkx	82	6	at	at	IN
cord-275016-ij5yaqkx	82	7	pH	pH	NNP
cord-275016-ij5yaqkx	82	8	8.62	8.62	CD
cord-275016-ij5yaqkx	82	9	is	be	VBZ
cord-275016-ij5yaqkx	82	10	100	100	CD
cord-275016-ij5yaqkx	82	11	%	%	NN
cord-275016-ij5yaqkx	82	12	,	,	,
cord-275016-ij5yaqkx	82	13	those	those	DT
cord-275016-ij5yaqkx	82	14	at	at	IN
cord-275016-ij5yaqkx	82	15	pH	pH	NNP
cord-275016-ij5yaqkx	82	16	7.22	7.22	CD
cord-275016-ij5yaqkx	82	17	,	,	,
cord-275016-ij5yaqkx	82	18	7.66	7.66	CD
cord-275016-ij5yaqkx	82	19	,	,	,
cord-275016-ij5yaqkx	82	20	8.12	8.12	CD
cord-275016-ij5yaqkx	82	21	,	,	,
cord-275016-ij5yaqkx	82	22	and	and	CC
cord-275016-ij5yaqkx	82	23	9.08	9.08	CD
cord-275016-ij5yaqkx	82	24	were	be	VBD
cord-275016-ij5yaqkx	82	25	estimated	estimate	VBN
cord-275016-ij5yaqkx	82	26	as	as	IN
cord-275016-ij5yaqkx	82	27	70	70	CD
cord-275016-ij5yaqkx	82	28	,	,	,
cord-275016-ij5yaqkx	82	29	86	86	CD
cord-275016-ij5yaqkx	82	30	,	,	,
cord-275016-ij5yaqkx	82	31	89	89	CD
cord-275016-ij5yaqkx	82	32	,	,	,
cord-275016-ij5yaqkx	82	33	and	and	CC
cord-275016-ij5yaqkx	82	34	79	79	CD
cord-275016-ij5yaqkx	82	35	%	%	NN
cord-275016-ij5yaqkx	82	36	,	,	,
cord-275016-ij5yaqkx	82	37	respectively	respectively	RB
cord-275016-ij5yaqkx	82	38	.	.	.
cord-275016-ij5yaqkx	83	1	A	a	DT
cord-275016-ij5yaqkx	83	2	slope	slope	NN
cord-275016-ij5yaqkx	83	3	of	of	IN
cord-275016-ij5yaqkx	83	4	relative	relative	JJ
cord-275016-ij5yaqkx	83	5	activity	activity	NN
cord-275016-ij5yaqkx	83	6	was	be	VBD
cord-275016-ij5yaqkx	83	7	gentle	gentle	JJ
cord-275016-ij5yaqkx	83	8	at	at	IN
cord-275016-ij5yaqkx	83	9	acidic	acidic	JJ
cord-275016-ij5yaqkx	83	10	pH	ph	NN
cord-275016-ij5yaqkx	83	11	below	below	IN
cord-275016-ij5yaqkx	83	12	8.62	8.62	CD
cord-275016-ij5yaqkx	83	13	,	,	,
cord-275016-ij5yaqkx	83	14	while	while	IN
cord-275016-ij5yaqkx	83	15	it	-PRON-	PRP
cord-275016-ij5yaqkx	83	16	was	be	VBD
cord-275016-ij5yaqkx	83	17	sharp	sharp	JJ
cord-275016-ij5yaqkx	83	18	at	at	IN
cord-275016-ij5yaqkx	83	19	alkaline	alkaline	JJ
cord-275016-ij5yaqkx	83	20	pH	ph	NN
cord-275016-ij5yaqkx	83	21	above	above	IN
cord-275016-ij5yaqkx	83	22	8.62	8.62	CD
cord-275016-ij5yaqkx	83	23	.	.	.
cord-275016-ij5yaqkx	84	1	In	in	IN
cord-275016-ij5yaqkx	84	2	the	the	DT
cord-275016-ij5yaqkx	84	3	subsequent	subsequent	JJ
cord-275016-ij5yaqkx	84	4	experiments	experiment	NNS
cord-275016-ij5yaqkx	84	5	,	,	,
cord-275016-ij5yaqkx	84	6	a	a	DT
cord-275016-ij5yaqkx	84	7	buffer	buffer	NN
cord-275016-ij5yaqkx	84	8	of	of	IN
cord-275016-ij5yaqkx	84	9	pH	pH	NNP
cord-275016-ij5yaqkx	84	10	8.62	8.62	CD
cord-275016-ij5yaqkx	84	11	was	be	VBD
cord-275016-ij5yaqkx	84	12	used	use	VBN
cord-275016-ij5yaqkx	84	13	.	.	.
cord-275016-ij5yaqkx	85	1	Five	five	CD
cord-275016-ij5yaqkx	85	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	85	3	enzyme	enzyme	NN
cord-275016-ij5yaqkx	85	4	and	and	CC
cord-275016-ij5yaqkx	85	5	5	5	CD
cord-275016-ij5yaqkx	85	6	M	m	NN
cord-275016-ij5yaqkx	85	7	substrate	substrate	NN
cord-275016-ij5yaqkx	85	8	were	be	VBD
cord-275016-ij5yaqkx	85	9	mixed	mix	VBN
cord-275016-ij5yaqkx	85	10	in	in	IN
cord-275016-ij5yaqkx	85	11	50	50	CD
cord-275016-ij5yaqkx	85	12	mM	mm	CD
cord-275016-ij5yaqkx	85	13	bis	bis	NN
cord-275016-ij5yaqkx	85	14	-	-	HYPH
cord-275016-ij5yaqkx	85	15	tris	tris	JJ
cord-275016-ij5yaqkx	85	16	-	-	HYPH
cord-275016-ij5yaqkx	85	17	propane	propane	NN
cord-275016-ij5yaqkx	85	18	-	-	HYPH
cord-275016-ij5yaqkx	85	19	HCl	HCl	NNP
cord-275016-ij5yaqkx	85	20	(	(	-LRB-
cord-275016-ij5yaqkx	85	21	pH	pH	NNP
cord-275016-ij5yaqkx	85	22	8.62	8.62	CD
cord-275016-ij5yaqkx	85	23	)	)	-RRB-
cord-275016-ij5yaqkx	85	24	containing	contain	VBG
cord-275016-ij5yaqkx	85	25	25	25	CD
cord-275016-ij5yaqkx	85	26	mM	mm	CD
cord-275016-ij5yaqkx	85	27	NaCl	nacl	NN
cord-275016-ij5yaqkx	85	28	,	,	,
cord-275016-ij5yaqkx	85	29	and	and	CC
cord-275016-ij5yaqkx	85	30	reacted	react	VBD
cord-275016-ij5yaqkx	85	31	for	for	IN
cord-275016-ij5yaqkx	85	32	16	16	CD
cord-275016-ij5yaqkx	85	33	h	h	NN
cord-275016-ij5yaqkx	85	34	at	at	IN
cord-275016-ij5yaqkx	85	35	4	4	CD
cord-275016-ij5yaqkx	85	36	,	,	,
cord-275016-ij5yaqkx	85	37	15	15	CD
cord-275016-ij5yaqkx	85	38	,	,	,
cord-275016-ij5yaqkx	85	39	26	26	CD
cord-275016-ij5yaqkx	85	40	,	,	,
cord-275016-ij5yaqkx	85	41	37	37	CD
cord-275016-ij5yaqkx	85	42	,	,	,
cord-275016-ij5yaqkx	85	43	or	or	CC
cord-275016-ij5yaqkx	85	44	48	48	CD
cord-275016-ij5yaqkx	85	45	•	•	NNS
cord-275016-ij5yaqkx	85	46	C.	c.	NN
cord-275016-ij5yaqkx	86	1	As	as	IN
cord-275016-ij5yaqkx	86	2	shown	show	VBN
cord-275016-ij5yaqkx	86	3	in	in	IN
cord-275016-ij5yaqkx	86	4	Fig	Fig	NNP
cord-275016-ij5yaqkx	86	5	.	.	.
cord-275016-ij5yaqkx	87	1	3	3	LS
cord-275016-ij5yaqkx	87	2	,	,	,
cord-275016-ij5yaqkx	87	3	optimal	optimal	JJ
cord-275016-ij5yaqkx	87	4	temperature	temperature	NN
cord-275016-ij5yaqkx	87	5	for	for	IN
cord-275016-ij5yaqkx	87	6	the	the	DT
cord-275016-ij5yaqkx	87	7	activity	activity	NN
cord-275016-ij5yaqkx	87	8	was	be	VBD
cord-275016-ij5yaqkx	87	9	37	37	CD
cord-275016-ij5yaqkx	87	10	•	•	NNS
cord-275016-ij5yaqkx	88	1	C.	C.	NNP
cord-275016-ij5yaqkx	89	1	The	the	DT
cord-275016-ij5yaqkx	89	2	activity	activity	NN
cord-275016-ij5yaqkx	89	3	at	at	IN
cord-275016-ij5yaqkx	89	4	4	4	CD
cord-275016-ij5yaqkx	89	5	,	,	,
cord-275016-ij5yaqkx	89	6	15	15	CD
cord-275016-ij5yaqkx	89	7	,	,	,
cord-275016-ij5yaqkx	89	8	26	26	CD
cord-275016-ij5yaqkx	89	9	,	,	,
cord-275016-ij5yaqkx	89	10	and	and	CC
cord-275016-ij5yaqkx	89	11	48	48	CD
cord-275016-ij5yaqkx	89	12	•	•	NNS
cord-275016-ij5yaqkx	89	13	C	C	NNP
cord-275016-ij5yaqkx	89	14	was	be	VBD
cord-275016-ij5yaqkx	89	15	estimated	estimate	VBN
cord-275016-ij5yaqkx	89	16	as	as	IN
cord-275016-ij5yaqkx	89	17	23	23	CD
cord-275016-ij5yaqkx	89	18	,	,	,
cord-275016-ij5yaqkx	89	19	31	31	CD
cord-275016-ij5yaqkx	89	20	,	,	,
cord-275016-ij5yaqkx	89	21	55	55	CD
cord-275016-ij5yaqkx	89	22	,	,	,
cord-275016-ij5yaqkx	89	23	and	and	CC
cord-275016-ij5yaqkx	89	24	80	80	CD
cord-275016-ij5yaqkx	89	25	%	%	NN
cord-275016-ij5yaqkx	89	26	of	of	IN
cord-275016-ij5yaqkx	89	27	that	that	DT
cord-275016-ij5yaqkx	89	28	at	at	IN
cord-275016-ij5yaqkx	89	29	37	37	CD
cord-275016-ij5yaqkx	89	30	•	•	NN
cord-275016-ij5yaqkx	89	31	C	c	NN
cord-275016-ij5yaqkx	89	32	,	,	,
cord-275016-ij5yaqkx	89	33	respectively	respectively	RB
cord-275016-ij5yaqkx	89	34	.	.	.
cord-275016-ij5yaqkx	89	35	 	 	_SP
cord-275016-ij5yaqkx	90	1	We	-PRON-	PRP
cord-275016-ij5yaqkx	90	2	next	next	RB
cord-275016-ij5yaqkx	90	3	examined	examine	VBD
cord-275016-ij5yaqkx	90	4	the	the	DT
cord-275016-ij5yaqkx	90	5	effects	effect	NNS
cord-275016-ij5yaqkx	90	6	of	of	IN
cord-275016-ij5yaqkx	90	7	monovalent	monovalent	NN
cord-275016-ij5yaqkx	90	8	and	and	CC
cord-275016-ij5yaqkx	90	9	divalent	divalent	JJ
cord-275016-ij5yaqkx	90	10	cations	cation	NNS
cord-275016-ij5yaqkx	90	11	on	on	IN
cord-275016-ij5yaqkx	90	12	the	the	DT
cord-275016-ij5yaqkx	90	13	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	90	14	activity	activity	NN
cord-275016-ij5yaqkx	90	15	.	.	.
cord-275016-ij5yaqkx	91	1	The	the	DT
cord-275016-ij5yaqkx	91	2	activity	activity	NN
cord-275016-ij5yaqkx	91	3	was	be	VBD
cord-275016-ij5yaqkx	91	4	compared	compare	VBN
cord-275016-ij5yaqkx	91	5	with	with	IN
cord-275016-ij5yaqkx	91	6	that	that	DT
cord-275016-ij5yaqkx	91	7	under	under	IN
cord-275016-ij5yaqkx	91	8	the	the	DT
cord-275016-ij5yaqkx	91	9	condition	condition	NN
cord-275016-ij5yaqkx	91	10	where	where	WRB
cord-275016-ij5yaqkx	91	11	50	50	CD
cord-275016-ij5yaqkx	91	12	mM	mm	NN
cord-275016-ij5yaqkx	91	13	bis	bis	NN
cord-275016-ij5yaqkx	91	14	-	-	HYPH
cord-275016-ij5yaqkx	91	15	tris	tris	JJ
cord-275016-ij5yaqkx	91	16	-	-	HYPH
cord-275016-ij5yaqkx	91	17	propane	propane	NN
cord-275016-ij5yaqkx	91	18	-	-	HYPH
cord-275016-ij5yaqkx	91	19	HCl	HCl	NNP
cord-275016-ij5yaqkx	91	20	(	(	-LRB-
cord-275016-ij5yaqkx	91	21	pH	pH	NNP
cord-275016-ij5yaqkx	91	22	8.62	8.62	CD
cord-275016-ij5yaqkx	91	23	)	)	-RRB-
cord-275016-ij5yaqkx	91	24	containing	contain	VBG
cord-275016-ij5yaqkx	91	25	5	5	CD
cord-275016-ij5yaqkx	91	26	mM	mm	CD
cord-275016-ij5yaqkx	91	27	EDTA	EDTA	NNP
cord-275016-ij5yaqkx	91	28	Fig	Fig	NNP
cord-275016-ij5yaqkx	91	29	.	.	.
cord-275016-ij5yaqkx	92	1	3	3	LS
cord-275016-ij5yaqkx	92	2	.	.	.
cord-275016-ij5yaqkx	93	1	Effects	effect	NNS
cord-275016-ij5yaqkx	93	2	of	of	IN
cord-275016-ij5yaqkx	93	3	temperature	temperature	NN
cord-275016-ij5yaqkx	93	4	on	on	IN
cord-275016-ij5yaqkx	93	5	protease	protease	NN
cord-275016-ij5yaqkx	93	6	activity	activity	NN
cord-275016-ij5yaqkx	93	7	.	.	.
cord-275016-ij5yaqkx	94	1	Five	five	CD
cord-275016-ij5yaqkx	94	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	94	3	enzyme	enzyme	NN
cord-275016-ij5yaqkx	94	4	(	(	-LRB-
cord-275016-ij5yaqkx	94	5	ProHis	ProHis	NNP
cord-275016-ij5yaqkx	94	6	)	)	-RRB-
cord-275016-ij5yaqkx	94	7	and	and	CC
cord-275016-ij5yaqkx	94	8	5	5	CD
cord-275016-ij5yaqkx	94	9	M	m	NN
cord-275016-ij5yaqkx	94	10	substrate	substrate	NN
cord-275016-ij5yaqkx	94	11	(	(	-LRB-
cord-275016-ij5yaqkx	94	12	His3Cd	His3Cd	NNP
cord-275016-ij5yaqkx	94	13	-	-	HYPH
cord-275016-ij5yaqkx	94	14	GST	GST	NNP
cord-275016-ij5yaqkx	94	15	)	)	-RRB-
cord-275016-ij5yaqkx	94	16	were	be	VBD
cord-275016-ij5yaqkx	94	17	reacted	react	VBN
cord-275016-ij5yaqkx	94	18	in	in	IN
cord-275016-ij5yaqkx	94	19	the	the	DT
cord-275016-ij5yaqkx	94	20	50	50	CD
cord-275016-ij5yaqkx	94	21	mM	mm	CD
cord-275016-ij5yaqkx	94	22	bis	bis	NN
cord-275016-ij5yaqkx	94	23	-	-	HYPH
cord-275016-ij5yaqkx	94	24	tris	tris	JJ
cord-275016-ij5yaqkx	94	25	-	-	HYPH
cord-275016-ij5yaqkx	94	26	propane	propane	NN
cord-275016-ij5yaqkx	94	27	-	-	HYPH
cord-275016-ij5yaqkx	94	28	HCl	HCl	NNP
cord-275016-ij5yaqkx	94	29	(	(	-LRB-
cord-275016-ij5yaqkx	94	30	pH	pH	NNP
cord-275016-ij5yaqkx	94	31	8.62	8.62	CD
cord-275016-ij5yaqkx	94	32	)	)	-RRB-
cord-275016-ij5yaqkx	94	33	buffer	buffer	NN
cord-275016-ij5yaqkx	94	34	containing	contain	VBG
cord-275016-ij5yaqkx	94	35	25	25	CD
cord-275016-ij5yaqkx	94	36	mM	mm	CD
cord-275016-ij5yaqkx	94	37	NaCl	nacl	NN
cord-275016-ij5yaqkx	94	38	for	for	IN
cord-275016-ij5yaqkx	94	39	16	16	CD
cord-275016-ij5yaqkx	94	40	h	h	NN
cord-275016-ij5yaqkx	94	41	at	at	IN
cord-275016-ij5yaqkx	94	42	the	the	DT
cord-275016-ij5yaqkx	94	43	indicated	indicate	VBN
cord-275016-ij5yaqkx	94	44	temperature	temperature	NN
cord-275016-ij5yaqkx	94	45	.	.	.
cord-275016-ij5yaqkx	95	1	without	without	IN
cord-275016-ij5yaqkx	95	2	any	any	DT
cord-275016-ij5yaqkx	95	3	cations	cation	NNS
cord-275016-ij5yaqkx	95	4	was	be	VBD
cord-275016-ij5yaqkx	95	5	used	use	VBN
cord-275016-ij5yaqkx	95	6	.	.	.
cord-275016-ij5yaqkx	96	1	The	the	DT
cord-275016-ij5yaqkx	96	2	addition	addition	NN
cord-275016-ij5yaqkx	96	3	of	of	IN
cord-275016-ij5yaqkx	96	4	25	25	CD
cord-275016-ij5yaqkx	96	5	,	,	,
cord-275016-ij5yaqkx	96	6	50	50	CD
cord-275016-ij5yaqkx	96	7	,	,	,
cord-275016-ij5yaqkx	96	8	or	or	CC
cord-275016-ij5yaqkx	96	9	100	100	CD
cord-275016-ij5yaqkx	96	10	mM	mm	CD
cord-275016-ij5yaqkx	96	11	NaCl	nacl	NN
cord-275016-ij5yaqkx	96	12	decreased	decrease	VBD
cord-275016-ij5yaqkx	96	13	the	the	DT
cord-275016-ij5yaqkx	96	14	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	96	15	activity	activity	NN
cord-275016-ij5yaqkx	96	16	to	to	IN
cord-275016-ij5yaqkx	96	17	83	83	CD
cord-275016-ij5yaqkx	96	18	,	,	,
cord-275016-ij5yaqkx	96	19	52	52	CD
cord-275016-ij5yaqkx	96	20	,	,	,
cord-275016-ij5yaqkx	96	21	or	or	CC
cord-275016-ij5yaqkx	96	22	36	36	CD
cord-275016-ij5yaqkx	96	23	%	%	NN
cord-275016-ij5yaqkx	96	24	,	,	,
cord-275016-ij5yaqkx	96	25	respectively	respectively	RB
cord-275016-ij5yaqkx	96	26	(	(	-LRB-
cord-275016-ij5yaqkx	96	27	Fig	Fig	NNP
cord-275016-ij5yaqkx	96	28	.	.	.
cord-275016-ij5yaqkx	96	29	4A	4a	CD
cord-275016-ij5yaqkx	96	30	)	)	-RRB-
cord-275016-ij5yaqkx	96	31	.	.	.
cord-275016-ij5yaqkx	97	1	KCl	KCl	NNP
cord-275016-ij5yaqkx	97	2	also	also	RB
cord-275016-ij5yaqkx	97	3	had	have	VBD
cord-275016-ij5yaqkx	97	4	an	an	DT
cord-275016-ij5yaqkx	97	5	inhibitory	inhibitory	JJ
cord-275016-ij5yaqkx	97	6	effect	effect	NN
cord-275016-ij5yaqkx	97	7	on	on	IN
cord-275016-ij5yaqkx	97	8	the	the	DT
cord-275016-ij5yaqkx	97	9	activity	activity	NN
cord-275016-ij5yaqkx	97	10	(	(	-LRB-
cord-275016-ij5yaqkx	97	11	data	datum	NNS
cord-275016-ij5yaqkx	97	12	not	not	RB
cord-275016-ij5yaqkx	97	13	shown	show	VBN
cord-275016-ij5yaqkx	97	14	)	)	-RRB-
cord-275016-ij5yaqkx	97	15	.	.	.
cord-275016-ij5yaqkx	98	1	As	as	IN
cord-275016-ij5yaqkx	98	2	for	for	IN
cord-275016-ij5yaqkx	98	3	the	the	DT
cord-275016-ij5yaqkx	98	4	divalent	divalent	JJ
cord-275016-ij5yaqkx	98	5	cations	cation	NNS
cord-275016-ij5yaqkx	98	6	,	,	,
cord-275016-ij5yaqkx	98	7	5	5	CD
cord-275016-ij5yaqkx	98	8	mM	mm	CD
cord-275016-ij5yaqkx	98	9	MgCl	MgCl	NNP
cord-275016-ij5yaqkx	98	10	2	2	CD
cord-275016-ij5yaqkx	98	11	or	or	CC
cord-275016-ij5yaqkx	98	12	5	5	CD
cord-275016-ij5yaqkx	98	13	mM	mm	CD
cord-275016-ij5yaqkx	98	14	CaCl	cacl	NN
cord-275016-ij5yaqkx	98	15	2	2	CD
cord-275016-ij5yaqkx	98	16	did	do	VBD
cord-275016-ij5yaqkx	98	17	not	not	RB
cord-275016-ij5yaqkx	98	18	affect	affect	VB
cord-275016-ij5yaqkx	98	19	the	the	DT
cord-275016-ij5yaqkx	98	20	activity	activity	NN
cord-275016-ij5yaqkx	98	21	(	(	-LRB-
cord-275016-ij5yaqkx	98	22	108	108	CD
cord-275016-ij5yaqkx	98	23	and	and	CC
cord-275016-ij5yaqkx	98	24	105	105	CD
cord-275016-ij5yaqkx	98	25	%	%	NN
cord-275016-ij5yaqkx	98	26	)	)	-RRB-
cord-275016-ij5yaqkx	99	1	(	(	-LRB-
cord-275016-ij5yaqkx	99	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	99	3	.	.	.
cord-275016-ij5yaqkx	99	4	4B	4b	CD
cord-275016-ij5yaqkx	99	5	)	)	-RRB-
cord-275016-ij5yaqkx	99	6	.	.	.
cord-275016-ij5yaqkx	100	1	In	in	IN
cord-275016-ij5yaqkx	100	2	contrast	contrast	NN
cord-275016-ij5yaqkx	100	3	,	,	,
cord-275016-ij5yaqkx	100	4	5	5	CD
cord-275016-ij5yaqkx	100	5	mM	mm	CD
cord-275016-ij5yaqkx	100	6	ZnCl	ZnCl	NNP
cord-275016-ij5yaqkx	100	7	2	2	CD
cord-275016-ij5yaqkx	100	8	or	or	CC
cord-275016-ij5yaqkx	100	9	5	5	CD
cord-275016-ij5yaqkx	100	10	mM	mm	CD
cord-275016-ij5yaqkx	100	11	HgCl	HgCl	NNS
cord-275016-ij5yaqkx	100	12	2	2	CD
cord-275016-ij5yaqkx	100	13	completely	completely	RB
cord-275016-ij5yaqkx	100	14	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	100	15	the	the	DT
cord-275016-ij5yaqkx	100	16	activity	activity	NN
cord-275016-ij5yaqkx	100	17	(	(	-LRB-
cord-275016-ij5yaqkx	100	18	Fig	Fig	NNP
cord-275016-ij5yaqkx	100	19	.	.	.
cord-275016-ij5yaqkx	100	20	4B	4b	CD
cord-275016-ij5yaqkx	100	21	)	)	-RRB-
cord-275016-ij5yaqkx	100	22	.	.	.
cord-275016-ij5yaqkx	101	1	This	this	DT
cord-275016-ij5yaqkx	101	2	result	result	NN
cord-275016-ij5yaqkx	101	3	was	be	VBD
cord-275016-ij5yaqkx	101	4	consistent	consistent	JJ
cord-275016-ij5yaqkx	101	5	with	with	IN
cord-275016-ij5yaqkx	101	6	Cys	Cys	NNP
cord-275016-ij5yaqkx	101	7	being	be	VBG
cord-275016-ij5yaqkx	101	8	a	a	DT
cord-275016-ij5yaqkx	101	9	critical	critical	JJ
cord-275016-ij5yaqkx	101	10	residue	residue	NN
cord-275016-ij5yaqkx	101	11	of	of	IN
cord-275016-ij5yaqkx	101	12	the	the	DT
cord-275016-ij5yaqkx	101	13	active	active	JJ
cord-275016-ij5yaqkx	101	14	site	site	NN
cord-275016-ij5yaqkx	102	1	(	(	-LRB-
cord-275016-ij5yaqkx	102	2	Someya	Someya	NNP
cord-275016-ij5yaqkx	102	3	et	et	NNP
cord-275016-ij5yaqkx	102	4	al	al	NNP
cord-275016-ij5yaqkx	102	5	.	.	NNP
cord-275016-ij5yaqkx	102	6	,	,	,
cord-275016-ij5yaqkx	102	7	2002	2002	CD
cord-275016-ij5yaqkx	102	8	)	)	-RRB-
cord-275016-ij5yaqkx	102	9	,	,	,
cord-275016-ij5yaqkx	102	10	and	and	CC
cord-275016-ij5yaqkx	102	11	inhibition	inhibition	NN
cord-275016-ij5yaqkx	102	12	by	by	IN
cord-275016-ij5yaqkx	102	13	Zn	Zn	NNP
cord-275016-ij5yaqkx	102	14	2	2	CD
cord-275016-ij5yaqkx	103	1	+	+	CC
cord-275016-ij5yaqkx	103	2	indicated	indicate	VBD
cord-275016-ij5yaqkx	103	3	that	that	IN
cord-275016-ij5yaqkx	103	4	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	103	5	3C	3c	NN
cord-275016-ij5yaqkx	103	6	-	-	HYPH
cord-275016-ij5yaqkx	103	7	like	like	JJ
cord-275016-ij5yaqkx	103	8	protease	protease	NN
cord-275016-ij5yaqkx	103	9	was	be	VBD
cord-275016-ij5yaqkx	103	10	different	different	JJ
cord-275016-ij5yaqkx	103	11	from	from	IN
cord-275016-ij5yaqkx	103	12	metalloprotease	metalloprotease	NNP
cord-275016-ij5yaqkx	103	13	.	.	.
cord-275016-ij5yaqkx	104	1	When	when	WRB
cord-275016-ij5yaqkx	104	2	Hg	Hg	NNP
cord-275016-ij5yaqkx	104	3	2	2	CD
cord-275016-ij5yaqkx	104	4	+	+	SYM
cord-275016-ij5yaqkx	104	5	was	be	VBD
cord-275016-ij5yaqkx	104	6	used	use	VBN
cord-275016-ij5yaqkx	104	7	,	,	,
cord-275016-ij5yaqkx	104	8	two	two	CD
cord-275016-ij5yaqkx	104	9	faint	faint	JJ
cord-275016-ij5yaqkx	104	10	bands	band	NNS
cord-275016-ij5yaqkx	104	11	of	of	IN
cord-275016-ij5yaqkx	104	12	30	30	CD
cord-275016-ij5yaqkx	104	13	and	and	CC
cord-275016-ij5yaqkx	104	14	15	15	CD
cord-275016-ij5yaqkx	104	15	kDa	kDa	NNPS
cord-275016-ij5yaqkx	104	16	slightly	slightly	RB
cord-275016-ij5yaqkx	104	17	appeared	appear	VBD
cord-275016-ij5yaqkx	104	18	(	(	-LRB-
cord-275016-ij5yaqkx	104	19	lane	lane	NN
cord-275016-ij5yaqkx	104	20	HgCl	HgCl	NNP
cord-275016-ij5yaqkx	104	21	2	2	CD
cord-275016-ij5yaqkx	104	22	,	,	,
cord-275016-ij5yaqkx	104	23	Fig	Fig	NNP
cord-275016-ij5yaqkx	104	24	.	.	.
cord-275016-ij5yaqkx	104	25	4B	4b	CD
cord-275016-ij5yaqkx	104	26	)	)	-RRB-
cord-275016-ij5yaqkx	104	27	.	.	.
cord-275016-ij5yaqkx	105	1	It	-PRON-	PRP
cord-275016-ij5yaqkx	105	2	was	be	VBD
cord-275016-ij5yaqkx	105	3	possible	possible	JJ
cord-275016-ij5yaqkx	105	4	that	that	IN
cord-275016-ij5yaqkx	105	5	high	high	JJ
cord-275016-ij5yaqkx	105	6	concentrations	concentration	NNS
cord-275016-ij5yaqkx	105	7	of	of	IN
cord-275016-ij5yaqkx	105	8	Hg	Hg	NNP
cord-275016-ij5yaqkx	105	9	2	2	CD
cord-275016-ij5yaqkx	105	10	+	+	SYM
cord-275016-ij5yaqkx	105	11	allowed	allow	VBD
cord-275016-ij5yaqkx	105	12	the	the	DT
cord-275016-ij5yaqkx	105	13	enzyme	enzyme	NN
cord-275016-ij5yaqkx	105	14	to	to	TO
cord-275016-ij5yaqkx	105	15	alternatively	alternatively	RB
cord-275016-ij5yaqkx	105	16	cleave	cleave	VB
cord-275016-ij5yaqkx	105	17	the	the	DT
cord-275016-ij5yaqkx	105	18	substrate	substrate	NN
cord-275016-ij5yaqkx	105	19	and/or	and/or	CC
cord-275016-ij5yaqkx	105	20	the	the	DT
cord-275016-ij5yaqkx	105	21	enzyme	enzyme	NN
cord-275016-ij5yaqkx	105	22	itself	-PRON-	PRP
cord-275016-ij5yaqkx	105	23	.	.	.
cord-275016-ij5yaqkx	106	1	With	with	IN
cord-275016-ij5yaqkx	106	2	the	the	DT
cord-275016-ij5yaqkx	106	3	addition	addition	NN
cord-275016-ij5yaqkx	106	4	of	of	IN
cord-275016-ij5yaqkx	106	5	5	5	CD
cord-275016-ij5yaqkx	106	6	mM	mm	CD
cord-275016-ij5yaqkx	106	7	MnCl	MnCl	NNP
cord-275016-ij5yaqkx	106	8	2	2	CD
cord-275016-ij5yaqkx	106	9	,	,	,
cord-275016-ij5yaqkx	106	10	part	part	NN
cord-275016-ij5yaqkx	106	11	of	of	IN
cord-275016-ij5yaqkx	106	12	the	the	DT
cord-275016-ij5yaqkx	106	13	proteins	protein	NNS
cord-275016-ij5yaqkx	106	14	used	use	VBN
cord-275016-ij5yaqkx	106	15	for	for	IN
cord-275016-ij5yaqkx	106	16	reaction	reaction	NN
cord-275016-ij5yaqkx	106	17	appeared	appear	VBD
cord-275016-ij5yaqkx	106	18	to	to	TO
cord-275016-ij5yaqkx	106	19	be	be	VB
cord-275016-ij5yaqkx	106	20	aggregated	aggregate	VBN
cord-275016-ij5yaqkx	106	21	since	since	IN
cord-275016-ij5yaqkx	106	22	Coomassiestained	Coomassiestained	NNP
cord-275016-ij5yaqkx	106	23	materials	material	NNS
cord-275016-ij5yaqkx	106	24	stayed	stay	VBD
cord-275016-ij5yaqkx	106	25	in	in	IN
cord-275016-ij5yaqkx	106	26	the	the	DT
cord-275016-ij5yaqkx	106	27	well	well	NN
cord-275016-ij5yaqkx	106	28	(	(	-LRB-
cord-275016-ij5yaqkx	106	29	Fig	fig	NN
cord-275016-ij5yaqkx	106	30	.	.	.
cord-275016-ij5yaqkx	106	31	4B	4b	CD
cord-275016-ij5yaqkx	106	32	)	)	-RRB-
cord-275016-ij5yaqkx	106	33	.	.	.
cord-275016-ij5yaqkx	107	1	Because	because	IN
cord-275016-ij5yaqkx	107	2	Mn	Mn	NNP
cord-275016-ij5yaqkx	107	3	2	2	CD
cord-275016-ij5yaqkx	107	4	+	+	SYM
cord-275016-ij5yaqkx	107	5	oxidized	oxidize	VBN
cord-275016-ij5yaqkx	107	6	the	the	DT
cord-275016-ij5yaqkx	107	7	proteins	protein	NNS
cord-275016-ij5yaqkx	107	8	,	,	,
cord-275016-ij5yaqkx	107	9	the	the	DT
cord-275016-ij5yaqkx	107	10	part	part	NN
cord-275016-ij5yaqkx	107	11	may	may	MD
cord-275016-ij5yaqkx	107	12	have	have	VB
cord-275016-ij5yaqkx	107	13	remained	remain	VBN
cord-275016-ij5yaqkx	107	14	in	in	IN
cord-275016-ij5yaqkx	107	15	the	the	DT
cord-275016-ij5yaqkx	107	16	well	well	NN
cord-275016-ij5yaqkx	107	17	and	and	CC
cord-275016-ij5yaqkx	107	18	the	the	DT
cord-275016-ij5yaqkx	107	19	other	other	JJ
cord-275016-ij5yaqkx	107	20	part	part	NN
cord-275016-ij5yaqkx	107	21	may	may	MD
cord-275016-ij5yaqkx	107	22	be	be	VB
cord-275016-ij5yaqkx	107	23	flown	fly	VBN
cord-275016-ij5yaqkx	107	24	from	from	IN
cord-275016-ij5yaqkx	107	25	the	the	DT
cord-275016-ij5yaqkx	107	26	gel	gel	NN
cord-275016-ij5yaqkx	107	27	after	after	IN
cord-275016-ij5yaqkx	107	28	degradation	degradation	NN
cord-275016-ij5yaqkx	107	29	into	into	IN
cord-275016-ij5yaqkx	107	30	small	small	JJ
cord-275016-ij5yaqkx	107	31	molecular	molecular	JJ
cord-275016-ij5yaqkx	107	32	weight	weight	NN
cord-275016-ij5yaqkx	107	33	peptides	peptide	NNS
cord-275016-ij5yaqkx	107	34	.	.	.
cord-275016-ij5yaqkx	108	1	Most	Most	JJS
cord-275016-ij5yaqkx	108	2	of	of	IN
cord-275016-ij5yaqkx	108	3	the	the	DT
cord-275016-ij5yaqkx	108	4	3C	3C	NNP
cord-275016-ij5yaqkx	108	5	and	and	CC
cord-275016-ij5yaqkx	108	6	3C	3C	NNP
cord-275016-ij5yaqkx	108	7	-	-	HYPH
cord-275016-ij5yaqkx	108	8	like	like	JJ
cord-275016-ij5yaqkx	108	9	proteases	protease	NNS
cord-275016-ij5yaqkx	108	10	have	have	VBP
cord-275016-ij5yaqkx	108	11	a	a	DT
cord-275016-ij5yaqkx	108	12	cysteine	cysteine	NN
cord-275016-ij5yaqkx	108	13	residue	residue	NN
cord-275016-ij5yaqkx	108	14	as	as	IN
cord-275016-ij5yaqkx	108	15	a	a	DT
cord-275016-ij5yaqkx	108	16	nucleophile	nucleophile	NN
cord-275016-ij5yaqkx	108	17	of	of	IN
cord-275016-ij5yaqkx	108	18	the	the	DT
cord-275016-ij5yaqkx	108	19	active	active	JJ
cord-275016-ij5yaqkx	108	20	site	site	NN
cord-275016-ij5yaqkx	108	21	(	(	-LRB-
cord-275016-ij5yaqkx	108	22	Dougherty	Dougherty	NNP
cord-275016-ij5yaqkx	108	23	and	and	CC
cord-275016-ij5yaqkx	108	24	Semler	Semler	NNP
cord-275016-ij5yaqkx	108	25	,	,	,
cord-275016-ij5yaqkx	108	26	1993	1993	CD
cord-275016-ij5yaqkx	108	27	;	;	:
cord-275016-ij5yaqkx	108	28	Gorbalenya	Gorbalenya	NNP
cord-275016-ij5yaqkx	108	29	et	et	NNP
cord-275016-ij5yaqkx	108	30	al	al	NNP
cord-275016-ij5yaqkx	108	31	.	.	NNP
cord-275016-ij5yaqkx	108	32	,	,	,
cord-275016-ij5yaqkx	108	33	1989	1989	CD
cord-275016-ij5yaqkx	108	34	)	)	-RRB-
cord-275016-ij5yaqkx	108	35	.	.	.
cord-275016-ij5yaqkx	109	1	These	these	DT
cord-275016-ij5yaqkx	109	2	proteases	protease	NNS
cord-275016-ij5yaqkx	109	3	should	should	MD
cord-275016-ij5yaqkx	109	4	therefore	therefore	RB
cord-275016-ij5yaqkx	109	5	be	be	VB
cord-275016-ij5yaqkx	109	6	sensitive	sensitive	JJ
cord-275016-ij5yaqkx	109	7	to	to	IN
cord-275016-ij5yaqkx	109	8	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	109	9	reagents	reagent	NNS
cord-275016-ij5yaqkx	109	10	.	.	.
cord-275016-ij5yaqkx	110	1	In	in	IN
cord-275016-ij5yaqkx	110	2	fact	fact	NN
cord-275016-ij5yaqkx	110	3	,	,	,
cord-275016-ij5yaqkx	110	4	as	as	IN
cord-275016-ij5yaqkx	110	5	shown	show	VBN
cord-275016-ij5yaqkx	110	6	in	in	IN
cord-275016-ij5yaqkx	110	7	Fig	Fig	NNP
cord-275016-ij5yaqkx	110	8	.	.	.
cord-275016-ij5yaqkx	110	9	4B	4b	CD
cord-275016-ij5yaqkx	110	10	,	,	,
cord-275016-ij5yaqkx	110	11	high	high	JJ
cord-275016-ij5yaqkx	110	12	concentrations	concentration	NNS
cord-275016-ij5yaqkx	110	13	(	(	-LRB-
cord-275016-ij5yaqkx	110	14	5	5	CD
cord-275016-ij5yaqkx	110	15	mM	mm	CD
cord-275016-ij5yaqkx	110	16	)	)	-RRB-
cord-275016-ij5yaqkx	110	17	of	of	IN
cord-275016-ij5yaqkx	110	18	Hg	Hg	NNP
cord-275016-ij5yaqkx	110	19	2	2	CD
cord-275016-ij5yaqkx	111	1	+	+	UH
cord-275016-ij5yaqkx	111	2	and	and	CC
cord-275016-ij5yaqkx	111	3	Zn	Zn	NNP
cord-275016-ij5yaqkx	111	4	2	2	CD
cord-275016-ij5yaqkx	112	1	+	+	SYM
cord-275016-ij5yaqkx	112	2	blocked	block	VBD
cord-275016-ij5yaqkx	112	3	the	the	DT
cord-275016-ij5yaqkx	112	4	activity	activity	NN
cord-275016-ij5yaqkx	112	5	of	of	IN
cord-275016-ij5yaqkx	112	6	the	the	DT
cord-275016-ij5yaqkx	112	7	3C	3c	NN
cord-275016-ij5yaqkx	112	8	-	-	HYPH
cord-275016-ij5yaqkx	112	9	like	like	JJ
cord-275016-ij5yaqkx	112	10	protease	protease	NN
cord-275016-ij5yaqkx	112	11	.	.	.
cord-275016-ij5yaqkx	113	1	In	in	IN
cord-275016-ij5yaqkx	113	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	113	3	.	.	.
cord-275016-ij5yaqkx	114	1	5	5	LS
cord-275016-ij5yaqkx	114	2	,	,	,
cord-275016-ij5yaqkx	114	3	the	the	DT
cord-275016-ij5yaqkx	114	4	effects	effect	NNS
cord-275016-ij5yaqkx	114	5	of	of	IN
cord-275016-ij5yaqkx	114	6	several	several	JJ
cord-275016-ij5yaqkx	114	7	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	114	8	reagents	reagent	NNS
cord-275016-ij5yaqkx	114	9	were	be	VBD
cord-275016-ij5yaqkx	114	10	tested	test	VBN
cord-275016-ij5yaqkx	114	11	.	.	.
cord-275016-ij5yaqkx	115	1	Five	five	CD
cord-275016-ij5yaqkx	115	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	115	3	substrate	substrate	NN
cord-275016-ij5yaqkx	115	4	was	be	VBD
cord-275016-ij5yaqkx	115	5	reacted	react	VBN
cord-275016-ij5yaqkx	115	6	with	with	IN
cord-275016-ij5yaqkx	115	7	5	5	CD
cord-275016-ij5yaqkx	115	8	M	M	NNP
cord-275016-ij5yaqkx	115	9	enzyme	enzyme	NN
cord-275016-ij5yaqkx	115	10	in	in	IN
cord-275016-ij5yaqkx	115	11	the	the	DT
cord-275016-ij5yaqkx	115	12	presence	presence	NN
cord-275016-ij5yaqkx	115	13	of	of	IN
cord-275016-ij5yaqkx	115	14	10	10	CD
cord-275016-ij5yaqkx	115	15	M	M	NNP
cord-275016-ij5yaqkx	115	16	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	115	17	reagents	reagent	NNS
cord-275016-ij5yaqkx	115	18	.	.	.
cord-275016-ij5yaqkx	116	1	Ten	ten	CD
cord-275016-ij5yaqkx	116	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	117	1	Hg	Hg	NNP
cord-275016-ij5yaqkx	117	2	2	2	CD
cord-275016-ij5yaqkx	117	3	+	+	SYM
cord-275016-ij5yaqkx	117	4	or	or	CC
cord-275016-ij5yaqkx	117	5	Zn	Zn	NNP
cord-275016-ij5yaqkx	117	6	2	2	CD
cord-275016-ij5yaqkx	117	7	+	+	CC
cord-275016-ij5yaqkx	118	1	almost	almost	RB
cord-275016-ij5yaqkx	118	2	completely	completely	RB
cord-275016-ij5yaqkx	118	3	in-	in-	JJ
cord-275016-ij5yaqkx	119	1	hibited	hibite	VBD
cord-275016-ij5yaqkx	119	2	the	the	DT
cord-275016-ij5yaqkx	119	3	activity	activity	NN
cord-275016-ij5yaqkx	119	4	.	.	.
cord-275016-ij5yaqkx	120	1	p	p	LS
cord-275016-ij5yaqkx	120	2	-	-	:
cord-275016-ij5yaqkx	120	3	Chloromercuribenzoate	Chloromercuribenzoate	NNP
cord-275016-ij5yaqkx	120	4	(	(	-LRB-
cord-275016-ij5yaqkx	120	5	PCMB	PCMB	NNP
cord-275016-ij5yaqkx	120	6	)	)	-RRB-
cord-275016-ij5yaqkx	120	7	,	,	,
cord-275016-ij5yaqkx	120	8	which	which	WDT
cord-275016-ij5yaqkx	120	9	includes	include	VBZ
cord-275016-ij5yaqkx	120	10	an	an	DT
cord-275016-ij5yaqkx	120	11	Hg(II	Hg(II	NNP
cord-275016-ij5yaqkx	120	12	)	)	-RRB-
cord-275016-ij5yaqkx	120	13	within	within	IN
cord-275016-ij5yaqkx	120	14	the	the	DT
cord-275016-ij5yaqkx	120	15	molecule	molecule	NN
cord-275016-ij5yaqkx	120	16	,	,	,
cord-275016-ij5yaqkx	120	17	was	be	VBD
cord-275016-ij5yaqkx	120	18	also	also	RB
cord-275016-ij5yaqkx	120	19	a	a	DT
cord-275016-ij5yaqkx	120	20	good	good	JJ
cord-275016-ij5yaqkx	120	21	inhibitor	inhibitor	NN
cord-275016-ij5yaqkx	120	22	of	of	IN
cord-275016-ij5yaqkx	120	23	the	the	DT
cord-275016-ij5yaqkx	120	24	3C	3c	NN
cord-275016-ij5yaqkx	120	25	-	-	HYPH
cord-275016-ij5yaqkx	120	26	like	like	JJ
cord-275016-ij5yaqkx	120	27	protease	protease	NN
cord-275016-ij5yaqkx	120	28	(	(	-LRB-
cord-275016-ij5yaqkx	120	29	82	82	CD
cord-275016-ij5yaqkx	120	30	%	%	NN
cord-275016-ij5yaqkx	120	31	inhibition	inhibition	NN
cord-275016-ij5yaqkx	120	32	)	)	-RRB-
cord-275016-ij5yaqkx	120	33	.	.	.
cord-275016-ij5yaqkx	121	1	In	in	IN
cord-275016-ij5yaqkx	121	2	contrast	contrast	NN
cord-275016-ij5yaqkx	121	3	,	,	,
cord-275016-ij5yaqkx	121	4	p	p	NN
cord-275016-ij5yaqkx	121	5	-	-	HYPH
cord-275016-ij5yaqkx	121	6	chloromercuribenzenesulfonate	chloromercuribenzenesulfonate	JJ
cord-275016-ij5yaqkx	121	7	(	(	-LRB-
cord-275016-ij5yaqkx	121	8	PCMBS	PCMBS	NNP
cord-275016-ij5yaqkx	121	9	)	)	-RRB-
cord-275016-ij5yaqkx	121	10	slightly	slightly	RB
cord-275016-ij5yaqkx	121	11	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	121	12	the	the	DT
cord-275016-ij5yaqkx	121	13	activity	activity	NN
cord-275016-ij5yaqkx	121	14	(	(	-LRB-
cord-275016-ij5yaqkx	121	15	27	27	CD
cord-275016-ij5yaqkx	121	16	%	%	NN
cord-275016-ij5yaqkx	121	17	inhibition	inhibition	NN
cord-275016-ij5yaqkx	121	18	)	)	-RRB-
cord-275016-ij5yaqkx	121	19	,	,	,
cord-275016-ij5yaqkx	121	20	despite	despite	IN
cord-275016-ij5yaqkx	121	21	PCMBS	PCMBS	NNP
cord-275016-ij5yaqkx	121	22	also	also	RB
cord-275016-ij5yaqkx	121	23	having	have	VBG
cord-275016-ij5yaqkx	121	24	an	an	DT
cord-275016-ij5yaqkx	121	25	Hg(II	Hg(II	NNP
cord-275016-ij5yaqkx	121	26	)	)	-RRB-
cord-275016-ij5yaqkx	121	27	similarly	similarly	RB
cord-275016-ij5yaqkx	121	28	to	to	IN
cord-275016-ij5yaqkx	121	29	PCMB	PCMB	NNP
cord-275016-ij5yaqkx	121	30	.	.	.
cord-275016-ij5yaqkx	122	1	Methyl	methyl	NN
cord-275016-ij5yaqkx	122	2	methanethiosulfonate	methanethiosulfonate	NN
cord-275016-ij5yaqkx	122	3	(	(	-LRB-
cord-275016-ij5yaqkx	122	4	MMTS	MMTS	NNP
cord-275016-ij5yaqkx	122	5	)	)	-RRB-
cord-275016-ij5yaqkx	122	6	,	,	,
cord-275016-ij5yaqkx	122	7	N	N	NNP
cord-275016-ij5yaqkx	122	8	-	-	HYPH
cord-275016-ij5yaqkx	122	9	ethylmaleimide	ethylmaleimide	NNP
cord-275016-ij5yaqkx	122	10	(	(	-LRB-
cord-275016-ij5yaqkx	122	11	NEM	NEM	NNP
cord-275016-ij5yaqkx	122	12	)	)	-RRB-
cord-275016-ij5yaqkx	122	13	,	,	,
cord-275016-ij5yaqkx	122	14	and	and	CC
cord-275016-ij5yaqkx	122	15	N	n	CD
cord-275016-ij5yaqkx	122	16	-	-	HYPH
cord-275016-ij5yaqkx	122	17	phenylmaleimide	phenylmaleimide	NNP
cord-275016-ij5yaqkx	122	18	(	(	-LRB-
cord-275016-ij5yaqkx	122	19	NPM	NPM	NNP
cord-275016-ij5yaqkx	122	20	)	)	-RRB-
cord-275016-ij5yaqkx	122	21	,	,	,
cord-275016-ij5yaqkx	122	22	which	which	WDT
cord-275016-ij5yaqkx	122	23	form	form	VBP
cord-275016-ij5yaqkx	122	24	a	a	DT
cord-275016-ij5yaqkx	122	25	covalent	covalent	JJ
cord-275016-ij5yaqkx	122	26	bond	bond	NN
cord-275016-ij5yaqkx	122	27	with	with	IN
cord-275016-ij5yaqkx	122	28	a	a	DT
cord-275016-ij5yaqkx	122	29	sulfur	sulfur	NN
cord-275016-ij5yaqkx	122	30	atom	atom	NN
cord-275016-ij5yaqkx	122	31	of	of	IN
cord-275016-ij5yaqkx	122	32	the	the	DT
cord-275016-ij5yaqkx	122	33	cysteine	cysteine	NN
cord-275016-ij5yaqkx	122	34	residue	residue	NN
cord-275016-ij5yaqkx	122	35	,	,	,
cord-275016-ij5yaqkx	122	36	almost	almost	RB
cord-275016-ij5yaqkx	122	37	completely	completely	RB
cord-275016-ij5yaqkx	122	38	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	122	39	the	the	DT
cord-275016-ij5yaqkx	122	40	activity	activity	NN
cord-275016-ij5yaqkx	122	41	.	.	.
cord-275016-ij5yaqkx	123	1	The	the	DT
cord-275016-ij5yaqkx	123	2	reaction	reaction	NN
cord-275016-ij5yaqkx	123	3	with	with	IN
cord-275016-ij5yaqkx	123	4	NEM	NEM	NNP
cord-275016-ij5yaqkx	123	5	and	and	CC
cord-275016-ij5yaqkx	123	6	NPM	NPM	NNP
cord-275016-ij5yaqkx	123	7	slightly	slightly	RB
cord-275016-ij5yaqkx	123	8	altered	alter	VBD
cord-275016-ij5yaqkx	123	9	the	the	DT
cord-275016-ij5yaqkx	123	10	mobility	mobility	NN
cord-275016-ij5yaqkx	123	11	of	of	IN
cord-275016-ij5yaqkx	123	12	the	the	DT
cord-275016-ij5yaqkx	123	13	enzyme	enzyme	NN
cord-275016-ij5yaqkx	123	14	molecules	molecule	NNS
cord-275016-ij5yaqkx	123	15	,	,	,
cord-275016-ij5yaqkx	123	16	possibly	possibly	RB
cord-275016-ij5yaqkx	123	17	because	because	IN
cord-275016-ij5yaqkx	123	18	a	a	DT
cord-275016-ij5yaqkx	123	19	bulkier	bulky	JJR
cord-275016-ij5yaqkx	123	20	modified	modify	VBN
cord-275016-ij5yaqkx	123	21	side	side	NN
cord-275016-ij5yaqkx	123	22	chain	chain	NN
cord-275016-ij5yaqkx	123	23	of	of	IN
cord-275016-ij5yaqkx	123	24	the	the	DT
cord-275016-ij5yaqkx	123	25	active	active	JJ
cord-275016-ij5yaqkx	123	26	-	-	HYPH
cord-275016-ij5yaqkx	123	27	site	site	NN
cord-275016-ij5yaqkx	123	28	cysteine	cysteine	NN
cord-275016-ij5yaqkx	123	29	residue	residue	NN
cord-275016-ij5yaqkx	123	30	affected	affect	VBD
cord-275016-ij5yaqkx	123	31	the	the	DT
cord-275016-ij5yaqkx	123	32	structure	structure	NN
cord-275016-ij5yaqkx	123	33	of	of	IN
cord-275016-ij5yaqkx	123	34	the	the	DT
cord-275016-ij5yaqkx	123	35	enzyme	enzyme	NN
cord-275016-ij5yaqkx	123	36	,	,	,
cord-275016-ij5yaqkx	123	37	or	or	CC
cord-275016-ij5yaqkx	123	38	because	because	IN
cord-275016-ij5yaqkx	123	39	more	more	JJR
cord-275016-ij5yaqkx	123	40	than	than	IN
cord-275016-ij5yaqkx	123	41	one	one	CD
cord-275016-ij5yaqkx	123	42	molecule	molecule	NN
cord-275016-ij5yaqkx	123	43	of	of	IN
cord-275016-ij5yaqkx	123	44	the	the	DT
cord-275016-ij5yaqkx	123	45	inhibitors	inhibitor	NNS
cord-275016-ij5yaqkx	123	46	attached	attach	VBN
cord-275016-ij5yaqkx	123	47	to	to	IN
cord-275016-ij5yaqkx	123	48	an	an	DT
cord-275016-ij5yaqkx	123	49	enzyme	enzyme	NN
cord-275016-ij5yaqkx	123	50	.	.	.
cord-275016-ij5yaqkx	124	1	Neither	neither	CC
cord-275016-ij5yaqkx	124	2	10	10	CD
cord-275016-ij5yaqkx	124	3	M	M	NNP
cord-275016-ij5yaqkx	124	4	iodoacetate	iodoacetate	NN
cord-275016-ij5yaqkx	124	5	(	(	-LRB-
cord-275016-ij5yaqkx	124	6	IAA	IAA	NNP
cord-275016-ij5yaqkx	124	7	)	)	-RRB-
cord-275016-ij5yaqkx	124	8	nor	nor	CC
cord-275016-ij5yaqkx	124	9	iodoacetamide	iodoacetamide	JJ
cord-275016-ij5yaqkx	124	10	(	(	-LRB-
cord-275016-ij5yaqkx	124	11	IAM	IAM	NNP
cord-275016-ij5yaqkx	124	12	)	)	-RRB-
cord-275016-ij5yaqkx	124	13	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	124	14	the	the	DT
cord-275016-ij5yaqkx	124	15	activity	activity	NN
cord-275016-ij5yaqkx	124	16	.	.	.
cord-275016-ij5yaqkx	125	1	These	these	DT
cord-275016-ij5yaqkx	125	2	two	two	CD
cord-275016-ij5yaqkx	125	3	reagents	reagent	NNS
cord-275016-ij5yaqkx	125	4	may	may	MD
cord-275016-ij5yaqkx	125	5	be	be	VB
cord-275016-ij5yaqkx	125	6	less	less	RBR
cord-275016-ij5yaqkx	125	7	sensitive	sensitive	JJ
cord-275016-ij5yaqkx	125	8	to	to	IN
cord-275016-ij5yaqkx	125	9	an	an	DT
cord-275016-ij5yaqkx	125	10	SH	sh	NN
cord-275016-ij5yaqkx	125	11	group	group	NN
cord-275016-ij5yaqkx	125	12	than	than	IN
cord-275016-ij5yaqkx	125	13	other	other	JJ
cord-275016-ij5yaqkx	125	14	modifying	modify	VBG
cord-275016-ij5yaqkx	125	15	reagents	reagent	NNS
cord-275016-ij5yaqkx	125	16	.	.	.
cord-275016-ij5yaqkx	126	1	Phenyl	Phenyl	NNP
cord-275016-ij5yaqkx	126	2	methylsulfonylfluoride	methylsulfonylfluoride	NN
cord-275016-ij5yaqkx	126	3	(	(	-LRB-
cord-275016-ij5yaqkx	126	4	PMSF	PMSF	NNP
cord-275016-ij5yaqkx	126	5	)	)	-RRB-
cord-275016-ij5yaqkx	126	6	is	be	VBZ
cord-275016-ij5yaqkx	126	7	well	well	RB
cord-275016-ij5yaqkx	126	8	known	know	VBN
cord-275016-ij5yaqkx	126	9	as	as	IN
cord-275016-ij5yaqkx	126	10	an	an	DT
cord-275016-ij5yaqkx	126	11	irreversible	irreversible	JJ
cord-275016-ij5yaqkx	126	12	inhibitor	inhibitor	NN
cord-275016-ij5yaqkx	126	13	of	of	IN
cord-275016-ij5yaqkx	126	14	serine	serine	JJ
cord-275016-ij5yaqkx	126	15	proteases	protease	NNS
cord-275016-ij5yaqkx	126	16	.	.	.
cord-275016-ij5yaqkx	127	1	Because	because	IN
cord-275016-ij5yaqkx	127	2	the	the	DT
cord-275016-ij5yaqkx	127	3	3C	3c	NN
cord-275016-ij5yaqkx	127	4	and	and	CC
cord-275016-ij5yaqkx	127	5	3C	3C	NNP
cord-275016-ij5yaqkx	127	6	-	-	HYPH
cord-275016-ij5yaqkx	127	7	like	like	JJ
cord-275016-ij5yaqkx	127	8	proteases	protease	NNS
cord-275016-ij5yaqkx	127	9	have	have	VBP
cord-275016-ij5yaqkx	127	10	a	a	DT
cord-275016-ij5yaqkx	127	11	structural	structural	JJ
cord-275016-ij5yaqkx	127	12	architecture	architecture	NN
cord-275016-ij5yaqkx	127	13	similar	similar	JJ
cord-275016-ij5yaqkx	127	14	to	to	IN
cord-275016-ij5yaqkx	127	15	chymotrypsin	chymotrypsin	NNP
cord-275016-ij5yaqkx	127	16	,	,	,
cord-275016-ij5yaqkx	127	17	and	and	CC
cord-275016-ij5yaqkx	127	18	with	with	IN
cord-275016-ij5yaqkx	127	19	essentially	essentially	RB
cord-275016-ij5yaqkx	127	20	the	the	DT
cord-275016-ij5yaqkx	127	21	same	same	JJ
cord-275016-ij5yaqkx	127	22	proteolysis	proteolysis	NN
cord-275016-ij5yaqkx	127	23	mechanism	mechanism	NN
cord-275016-ij5yaqkx	127	24	(	(	-LRB-
cord-275016-ij5yaqkx	127	25	Dougherty	Dougherty	NNP
cord-275016-ij5yaqkx	127	26	and	and	CC
cord-275016-ij5yaqkx	127	27	Semler	Semler	NNP
cord-275016-ij5yaqkx	127	28	,	,	,
cord-275016-ij5yaqkx	127	29	1993	1993	CD
cord-275016-ij5yaqkx	127	30	;	;	:
cord-275016-ij5yaqkx	127	31	Gorbalenya	Gorbalenya	NNP
cord-275016-ij5yaqkx	127	32	et	et	NNP
cord-275016-ij5yaqkx	127	33	al	al	NNP
cord-275016-ij5yaqkx	127	34	.	.	NNP
cord-275016-ij5yaqkx	127	35	,	,	,
cord-275016-ij5yaqkx	127	36	1989	1989	CD
cord-275016-ij5yaqkx	127	37	)	)	-RRB-
cord-275016-ij5yaqkx	127	38	,	,	,
cord-275016-ij5yaqkx	127	39	PMSF	PMSF	NNP
cord-275016-ij5yaqkx	127	40	may	may	MD
cord-275016-ij5yaqkx	127	41	inhibit	inhibit	VB
cord-275016-ij5yaqkx	127	42	the	the	DT
cord-275016-ij5yaqkx	127	43	activity	activity	NN
cord-275016-ij5yaqkx	127	44	of	of	IN
cord-275016-ij5yaqkx	127	45	the	the	DT
cord-275016-ij5yaqkx	127	46	3C	3c	NN
cord-275016-ij5yaqkx	127	47	and	and	CC
cord-275016-ij5yaqkx	127	48	3C	3C	NNP
cord-275016-ij5yaqkx	127	49	-	-	HYPH
cord-275016-ij5yaqkx	127	50	like	like	JJ
cord-275016-ij5yaqkx	127	51	proteases	protease	NNS
cord-275016-ij5yaqkx	127	52	.	.	.
cord-275016-ij5yaqkx	128	1	Ten	ten	CD
cord-275016-ij5yaqkx	128	2	micromolar	micromolar	JJ
cord-275016-ij5yaqkx	128	3	PMSF	PMSF	NNP
cord-275016-ij5yaqkx	128	4	was	be	VBD
cord-275016-ij5yaqkx	128	5	less	less	RBR
cord-275016-ij5yaqkx	128	6	effective	effective	JJ
cord-275016-ij5yaqkx	128	7	but	but	CC
cord-275016-ij5yaqkx	128	8	significantly	significantly	RB
cord-275016-ij5yaqkx	128	9	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	128	10	the	the	DT
cord-275016-ij5yaqkx	128	11	activity	activity	NN
cord-275016-ij5yaqkx	128	12	of	of	IN
cord-275016-ij5yaqkx	128	13	the	the	DT
cord-275016-ij5yaqkx	128	14	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	128	15	virus	virus	NN
cord-275016-ij5yaqkx	128	16	3C	3c	NN
cord-275016-ij5yaqkx	128	17	-	-	HYPH
cord-275016-ij5yaqkx	128	18	like	like	JJ
cord-275016-ij5yaqkx	128	19	protease	protease	NN
cord-275016-ij5yaqkx	128	20	(	(	-LRB-
cord-275016-ij5yaqkx	128	21	20	20	CD
cord-275016-ij5yaqkx	128	22	%	%	NN
cord-275016-ij5yaqkx	128	23	inhibition	inhibition	NN
cord-275016-ij5yaqkx	128	24	)	)	-RRB-
cord-275016-ij5yaqkx	128	25	.	.	.
cord-275016-ij5yaqkx	129	1	The	the	DT
cord-275016-ij5yaqkx	129	2	effect	effect	NN
cord-275016-ij5yaqkx	129	3	of	of	IN
cord-275016-ij5yaqkx	129	4	␤	␤	NNP
cord-275016-ij5yaqkx	129	5	-mercaptoethanol	-mercaptoethanol	NN
cord-275016-ij5yaqkx	129	6	(	(	-LRB-
cord-275016-ij5yaqkx	129	7	␤	␤	DT
cord-275016-ij5yaqkx	129	8	-ME	-ME	NNP
cord-275016-ij5yaqkx	129	9	)	)	-RRB-
cord-275016-ij5yaqkx	129	10	was	be	VBD
cord-275016-ij5yaqkx	129	11	also	also	RB
cord-275016-ij5yaqkx	129	12	examined	examine	VBN
cord-275016-ij5yaqkx	129	13	here	here	RB
cord-275016-ij5yaqkx	129	14	.	.	.
cord-275016-ij5yaqkx	130	1	The	the	DT
cord-275016-ij5yaqkx	130	2	residual	residual	JJ
cord-275016-ij5yaqkx	130	3	activity	activity	NN
cord-275016-ij5yaqkx	130	4	of	of	IN
cord-275016-ij5yaqkx	130	5	the	the	DT
cord-275016-ij5yaqkx	130	6	3C	3c	NN
cord-275016-ij5yaqkx	130	7	-	-	HYPH
cord-275016-ij5yaqkx	130	8	like	like	JJ
cord-275016-ij5yaqkx	130	9	protease	protease	NN
cord-275016-ij5yaqkx	130	10	was	be	VBD
cord-275016-ij5yaqkx	130	11	approximately	approximately	RB
cord-275016-ij5yaqkx	130	12	80	80	CD
cord-275016-ij5yaqkx	130	13	%	%	NN
cord-275016-ij5yaqkx	130	14	of	of	IN
cord-275016-ij5yaqkx	130	15	the	the	DT
cord-275016-ij5yaqkx	130	16	control	control	NN
cord-275016-ij5yaqkx	130	17	in	in	IN
cord-275016-ij5yaqkx	130	18	the	the	DT
cord-275016-ij5yaqkx	130	19	presence	presence	NN
cord-275016-ij5yaqkx	130	20	of	of	IN
cord-275016-ij5yaqkx	130	21	10	10	CD
cord-275016-ij5yaqkx	130	22	M	m	NN
cord-275016-ij5yaqkx	130	23	␤	␤	.
cord-275016-ij5yaqkx	131	1	-ME	-ME	NNP
cord-275016-ij5yaqkx	132	1	(	(	-LRB-
cord-275016-ij5yaqkx	132	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	132	3	.	.	NNP
cord-275016-ij5yaqkx	132	4	5	5	CD
cord-275016-ij5yaqkx	132	5	)	)	-RRB-
cord-275016-ij5yaqkx	132	6	.	.	.
cord-275016-ij5yaqkx	133	1	Even	even	RB
cord-275016-ij5yaqkx	133	2	in	in	IN
cord-275016-ij5yaqkx	133	3	the	the	DT
cord-275016-ij5yaqkx	133	4	presence	presence	NN
cord-275016-ij5yaqkx	133	5	of	of	IN
cord-275016-ij5yaqkx	133	6	much	much	RB
cord-275016-ij5yaqkx	133	7	higher	high	JJR
cord-275016-ij5yaqkx	133	8	concentrations	concentration	NNS
cord-275016-ij5yaqkx	133	9	(	(	-LRB-
cord-275016-ij5yaqkx	133	10	5	5	CD
cord-275016-ij5yaqkx	133	11	mM	mm	CD
cord-275016-ij5yaqkx	133	12	)	)	-RRB-
cord-275016-ij5yaqkx	133	13	of	of	IN
cord-275016-ij5yaqkx	133	14	␤	␤	IN
cord-275016-ij5yaqkx	133	15	-ME	-ME	NNP
cord-275016-ij5yaqkx	133	16	,	,	,
cord-275016-ij5yaqkx	133	17	approximately	approximately	RB
cord-275016-ij5yaqkx	133	18	60	60	CD
cord-275016-ij5yaqkx	133	19	%	%	NN
cord-275016-ij5yaqkx	133	20	activity	activity	NN
cord-275016-ij5yaqkx	133	21	remained	remain	VBD
cord-275016-ij5yaqkx	133	22	(	(	-LRB-
cord-275016-ij5yaqkx	133	23	data	datum	NNS
cord-275016-ij5yaqkx	133	24	not	not	RB
cord-275016-ij5yaqkx	133	25	shown	show	VBN
cord-275016-ij5yaqkx	133	26	)	)	-RRB-
cord-275016-ij5yaqkx	133	27	.	.	.
cord-275016-ij5yaqkx	134	1	These	these	DT
cord-275016-ij5yaqkx	134	2	results	result	NNS
cord-275016-ij5yaqkx	134	3	suggest	suggest	VBP
cord-275016-ij5yaqkx	134	4	that	that	IN
cord-275016-ij5yaqkx	134	5	a	a	DT
cord-275016-ij5yaqkx	134	6	disulfide	disulfide	JJ
cord-275016-ij5yaqkx	134	7	bond	bond	NN
cord-275016-ij5yaqkx	134	8	is	be	VBZ
cord-275016-ij5yaqkx	134	9	not	not	RB
cord-275016-ij5yaqkx	134	10	essential	essential	JJ
cord-275016-ij5yaqkx	134	11	to	to	IN
cord-275016-ij5yaqkx	134	12	the	the	DT
cord-275016-ij5yaqkx	134	13	activity	activity	NN
cord-275016-ij5yaqkx	134	14	.	.	.
cord-275016-ij5yaqkx	135	1	However	however	RB
cord-275016-ij5yaqkx	135	2	,	,	,
cord-275016-ij5yaqkx	135	3	we	-PRON-	PRP
cord-275016-ij5yaqkx	135	4	can	can	MD
cord-275016-ij5yaqkx	135	5	not	not	RB
cord-275016-ij5yaqkx	135	6	exclude	exclude	VB
cord-275016-ij5yaqkx	135	7	the	the	DT
cord-275016-ij5yaqkx	135	8	possibility	possibility	NN
cord-275016-ij5yaqkx	135	9	that	that	IN
cord-275016-ij5yaqkx	135	10	a	a	DT
cord-275016-ij5yaqkx	135	11	disulfide	disulfide	JJ
cord-275016-ij5yaqkx	135	12	bond	bond	NN
cord-275016-ij5yaqkx	135	13	is	be	VBZ
cord-275016-ij5yaqkx	135	14	buried	bury	VBN
cord-275016-ij5yaqkx	135	15	inside	inside	IN
cord-275016-ij5yaqkx	135	16	the	the	DT
cord-275016-ij5yaqkx	135	17	folded	fold	VBN
cord-275016-ij5yaqkx	135	18	protein	protein	NN
cord-275016-ij5yaqkx	135	19	and	and	CC
cord-275016-ij5yaqkx	135	20	can	can	MD
cord-275016-ij5yaqkx	135	21	not	not	RB
cord-275016-ij5yaqkx	135	22	be	be	VB
cord-275016-ij5yaqkx	135	23	reduced	reduce	VBN
cord-275016-ij5yaqkx	135	24	by	by	IN
cord-275016-ij5yaqkx	135	25	high	high	JJ
cord-275016-ij5yaqkx	135	26	concentrations	concentration	NNS
cord-275016-ij5yaqkx	135	27	of	of	IN
cord-275016-ij5yaqkx	135	28	␤	␤	CD
cord-275016-ij5yaqkx	135	29	-ME	-me	NN
cord-275016-ij5yaqkx	135	30	.	.	.
cord-275016-ij5yaqkx	136	1	The	the	DT
cord-275016-ij5yaqkx	136	2	optimal	optimal	JJ
cord-275016-ij5yaqkx	136	3	pH	ph	NN
cord-275016-ij5yaqkx	136	4	for	for	IN
cord-275016-ij5yaqkx	136	5	the	the	DT
cord-275016-ij5yaqkx	136	6	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	136	7	reaction	reaction	NN
cord-275016-ij5yaqkx	136	8	by	by	IN
cord-275016-ij5yaqkx	136	9	the	the	DT
cord-275016-ij5yaqkx	136	10	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	136	11	virus	virus	NN
cord-275016-ij5yaqkx	136	12	3C	3c	NN
cord-275016-ij5yaqkx	136	13	-	-	HYPH
cord-275016-ij5yaqkx	136	14	like	like	JJ
cord-275016-ij5yaqkx	136	15	protease	protease	NN
cord-275016-ij5yaqkx	136	16	was	be	VBD
cord-275016-ij5yaqkx	136	17	around	around	RB
cord-275016-ij5yaqkx	136	18	8.6	8.6	CD
cord-275016-ij5yaqkx	136	19	,	,	,
cord-275016-ij5yaqkx	136	20	which	which	WDT
cord-275016-ij5yaqkx	136	21	is	be	VBZ
cord-275016-ij5yaqkx	136	22	much	much	RB
cord-275016-ij5yaqkx	136	23	more	more	RBR
cord-275016-ij5yaqkx	136	24	alkaline	alkaline	JJ
cord-275016-ij5yaqkx	136	25	than	than	IN
cord-275016-ij5yaqkx	136	26	physiological	physiological	JJ
cord-275016-ij5yaqkx	136	27	pH	ph	NN
cord-275016-ij5yaqkx	137	1	(	(	-LRB-
cord-275016-ij5yaqkx	137	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	137	3	.	.	NNP
cord-275016-ij5yaqkx	137	4	2	2	CD
cord-275016-ij5yaqkx	137	5	)	)	-RRB-
cord-275016-ij5yaqkx	137	6	.	.	.
cord-275016-ij5yaqkx	138	1	The	the	DT
cord-275016-ij5yaqkx	138	2	3C	3c	NN
cord-275016-ij5yaqkx	138	3	-	-	HYPH
cord-275016-ij5yaqkx	138	4	like	like	JJ
cord-275016-ij5yaqkx	138	5	protease	protease	NN
cord-275016-ij5yaqkx	138	6	was	be	VBD
cord-275016-ij5yaqkx	138	7	sensitive	sensitive	JJ
cord-275016-ij5yaqkx	138	8	to	to	IN
cord-275016-ij5yaqkx	138	9	increased	increase	VBN
cord-275016-ij5yaqkx	138	10	concentrations	concentration	NNS
cord-275016-ij5yaqkx	138	11	of	of	IN
cord-275016-ij5yaqkx	138	12	Na	Na	NNP
cord-275016-ij5yaqkx	138	13	+	+	CC
cord-275016-ij5yaqkx	139	1	(	(	-LRB-
cord-275016-ij5yaqkx	139	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	139	3	.	.	.
cord-275016-ij5yaqkx	139	4	4A	4a	CD
cord-275016-ij5yaqkx	139	5	)	)	-RRB-
cord-275016-ij5yaqkx	139	6	.	.	.
cord-275016-ij5yaqkx	140	1	In	in	IN
cord-275016-ij5yaqkx	140	2	addition	addition	NN
cord-275016-ij5yaqkx	140	3	,	,	,
cord-275016-ij5yaqkx	140	4	high	high	JJ
cord-275016-ij5yaqkx	140	5	concentrations	concentration	NNS
cord-275016-ij5yaqkx	140	6	of	of	IN
cord-275016-ij5yaqkx	140	7	K	K	NNP
cord-275016-ij5yaqkx	140	8	+	+	CC
cord-275016-ij5yaqkx	140	9	,	,	,
cord-275016-ij5yaqkx	140	10	which	which	WDT
cord-275016-ij5yaqkx	140	11	is	be	VBZ
cord-275016-ij5yaqkx	140	12	abundant	abundant	JJ
cord-275016-ij5yaqkx	140	13	inside	inside	IN
cord-275016-ij5yaqkx	140	14	the	the	DT
cord-275016-ij5yaqkx	140	15	cell	cell	NN
cord-275016-ij5yaqkx	140	16	in	in	IN
cord-275016-ij5yaqkx	140	17	a	a	DT
cord-275016-ij5yaqkx	140	18	normal	normal	JJ
cord-275016-ij5yaqkx	140	19	state	state	NN
cord-275016-ij5yaqkx	140	20	,	,	,
cord-275016-ij5yaqkx	140	21	were	be	VBD
cord-275016-ij5yaqkx	140	22	also	also	RB
cord-275016-ij5yaqkx	140	23	inhibitory	inhibitory	JJ
cord-275016-ij5yaqkx	140	24	to	to	IN
cord-275016-ij5yaqkx	140	25	the	the	DT
cord-275016-ij5yaqkx	140	26	activity	activity	NN
cord-275016-ij5yaqkx	140	27	(	(	-LRB-
cord-275016-ij5yaqkx	140	28	data	datum	NNS
cord-275016-ij5yaqkx	140	29	not	not	RB
cord-275016-ij5yaqkx	140	30	shown	show	VBN
cord-275016-ij5yaqkx	140	31	)	)	-RRB-
cord-275016-ij5yaqkx	140	32	.	.	.
cord-275016-ij5yaqkx	141	1	The	the	DT
cord-275016-ij5yaqkx	141	2	presence	presence	NN
cord-275016-ij5yaqkx	141	3	of	of	IN
cord-275016-ij5yaqkx	141	4	Mg	Mg	NNP
cord-275016-ij5yaqkx	141	5	2	2	CD
cord-275016-ij5yaqkx	141	6	+	+	SYM
cord-275016-ij5yaqkx	141	7	or	or	CC
cord-275016-ij5yaqkx	141	8	Ca	Ca	NNP
cord-275016-ij5yaqkx	141	9	2	2	CD
cord-275016-ij5yaqkx	141	10	+	+	CC
cord-275016-ij5yaqkx	141	11	did	do	VBD
cord-275016-ij5yaqkx	141	12	not	not	RB
cord-275016-ij5yaqkx	141	13	affect	affect	VB
cord-275016-ij5yaqkx	141	14	the	the	DT
cord-275016-ij5yaqkx	141	15	activity	activity	NN
cord-275016-ij5yaqkx	141	16	at	at	RB
cord-275016-ij5yaqkx	141	17	all	all	DT
cord-275016-ij5yaqkx	141	18	(	(	-LRB-
cord-275016-ij5yaqkx	141	19	Fig	fig	NN
cord-275016-ij5yaqkx	141	20	.	.	.
cord-275016-ij5yaqkx	141	21	4B	4b	CD
cord-275016-ij5yaqkx	141	22	)	)	-RRB-
cord-275016-ij5yaqkx	141	23	.	.	.
cord-275016-ij5yaqkx	142	1	These	these	DT
cord-275016-ij5yaqkx	142	2	features	feature	NNS
cord-275016-ij5yaqkx	142	3	might	may	MD
cord-275016-ij5yaqkx	142	4	suggest	suggest	VB
cord-275016-ij5yaqkx	142	5	that	that	IN
cord-275016-ij5yaqkx	142	6	the	the	DT
cord-275016-ij5yaqkx	142	7	3C	3c	NN
cord-275016-ij5yaqkx	142	8	-	-	HYPH
cord-275016-ij5yaqkx	142	9	like	like	JJ
cord-275016-ij5yaqkx	142	10	protease	protease	NN
cord-275016-ij5yaqkx	142	11	is	be	VBZ
cord-275016-ij5yaqkx	142	12	localized	localize	VBN
cord-275016-ij5yaqkx	142	13	and	and	CC
cord-275016-ij5yaqkx	142	14	works	work	VBZ
cord-275016-ij5yaqkx	142	15	in	in	IN
cord-275016-ij5yaqkx	142	16	a	a	DT
cord-275016-ij5yaqkx	142	17	limited	limited	JJ
cord-275016-ij5yaqkx	142	18	environment	environment	NN
cord-275016-ij5yaqkx	142	19	inside	inside	IN
cord-275016-ij5yaqkx	142	20	the	the	DT
cord-275016-ij5yaqkx	142	21	infected	infected	JJ
cord-275016-ij5yaqkx	142	22	cells	cell	NNS
cord-275016-ij5yaqkx	142	23	,	,	,
cord-275016-ij5yaqkx	142	24	rather	rather	RB
cord-275016-ij5yaqkx	142	25	than	than	IN
cord-275016-ij5yaqkx	142	26	spreading	spread	VBG
cord-275016-ij5yaqkx	142	27	throughout	throughout	IN
cord-275016-ij5yaqkx	142	28	the	the	DT
cord-275016-ij5yaqkx	142	29	cells	cell	NNS
cord-275016-ij5yaqkx	142	30	.	.	.
cord-275016-ij5yaqkx	143	1	More	More	JJR
cord-275016-ij5yaqkx	143	2	information	information	NN
cord-275016-ij5yaqkx	143	3	is	be	VBZ
cord-275016-ij5yaqkx	143	4	necessary	necessary	JJ
cord-275016-ij5yaqkx	143	5	to	to	TO
cord-275016-ij5yaqkx	143	6	elucidate	elucidate	VB
cord-275016-ij5yaqkx	143	7	when	when	WRB
cord-275016-ij5yaqkx	143	8	and	and	CC
cord-275016-ij5yaqkx	143	9	where	where	WRB
cord-275016-ij5yaqkx	143	10	the	the	DT
cord-275016-ij5yaqkx	143	11	3C	3c	NN
cord-275016-ij5yaqkx	143	12	-	-	HYPH
cord-275016-ij5yaqkx	143	13	like	like	JJ
cord-275016-ij5yaqkx	143	14	protease	protease	NN
cord-275016-ij5yaqkx	143	15	does	do	VBZ
cord-275016-ij5yaqkx	143	16	work	work	NN
cord-275016-ij5yaqkx	143	17	in	in	IN
cord-275016-ij5yaqkx	143	18	the	the	DT
cord-275016-ij5yaqkx	143	19	infected	infected	JJ
cord-275016-ij5yaqkx	143	20	cell	cell	NN
cord-275016-ij5yaqkx	143	21	.	.	.
cord-275016-ij5yaqkx	144	1	We	-PRON-	PRP
cord-275016-ij5yaqkx	144	2	have	have	VBP
cord-275016-ij5yaqkx	144	3	previously	previously	RB
cord-275016-ij5yaqkx	144	4	shown	show	VBN
cord-275016-ij5yaqkx	144	5	that	that	IN
cord-275016-ij5yaqkx	144	6	the	the	DT
cord-275016-ij5yaqkx	144	7	active	active	JJ
cord-275016-ij5yaqkx	144	8	site	site	NN
cord-275016-ij5yaqkx	144	9	of	of	IN
cord-275016-ij5yaqkx	144	10	the	the	DT
cord-275016-ij5yaqkx	144	11	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	144	12	virus	virus	NN
cord-275016-ij5yaqkx	144	13	3C	3c	NN
cord-275016-ij5yaqkx	144	14	-	-	HYPH
cord-275016-ij5yaqkx	144	15	like	like	JJ
cord-275016-ij5yaqkx	144	16	protease	protease	NN
cord-275016-ij5yaqkx	144	17	is	be	VBZ
cord-275016-ij5yaqkx	144	18	composed	compose	VBN
cord-275016-ij5yaqkx	144	19	of	of	IN
cord-275016-ij5yaqkx	144	20	Cys139	cys139	CD
cord-275016-ij5yaqkx	144	21	and	and	CC
cord-275016-ij5yaqkx	144	22	His30	His30	NNP
cord-275016-ij5yaqkx	144	23	(	(	-LRB-
cord-275016-ij5yaqkx	144	24	Someya	Someya	NNP
cord-275016-ij5yaqkx	144	25	et	et	NNP
cord-275016-ij5yaqkx	144	26	al	al	NNP
cord-275016-ij5yaqkx	144	27	.	.	NNP
cord-275016-ij5yaqkx	144	28	,	,	,
cord-275016-ij5yaqkx	144	29	2002	2002	CD
cord-275016-ij5yaqkx	144	30	)	)	-RRB-
cord-275016-ij5yaqkx	144	31	.	.	.
cord-275016-ij5yaqkx	145	1	As	as	IN
cord-275016-ij5yaqkx	145	2	expected	expect	VBN
cord-275016-ij5yaqkx	145	3	,	,	,
cord-275016-ij5yaqkx	145	4	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	145	5	reagents	reagent	NNS
cord-275016-ij5yaqkx	145	6	as	as	RB
cord-275016-ij5yaqkx	145	7	well	well	RB
cord-275016-ij5yaqkx	145	8	as	as	IN
cord-275016-ij5yaqkx	145	9	Hg	Hg	NNP
cord-275016-ij5yaqkx	145	10	2	2	CD
cord-275016-ij5yaqkx	145	11	+	+	SYM
cord-275016-ij5yaqkx	145	12	and	and	CC
cord-275016-ij5yaqkx	145	13	Zn	Zn	NNP
cord-275016-ij5yaqkx	145	14	2	2	CD
cord-275016-ij5yaqkx	145	15	+	+	CC
cord-275016-ij5yaqkx	145	16	inhibited	inhibit	VBD
cord-275016-ij5yaqkx	145	17	the	the	DT
cord-275016-ij5yaqkx	145	18	activity	activity	NN
cord-275016-ij5yaqkx	145	19	of	of	IN
cord-275016-ij5yaqkx	145	20	the	the	DT
cord-275016-ij5yaqkx	145	21	3C	3c	NN
cord-275016-ij5yaqkx	145	22	-	-	HYPH
cord-275016-ij5yaqkx	145	23	like	like	JJ
cord-275016-ij5yaqkx	145	24	protease	protease	NN
cord-275016-ij5yaqkx	145	25	,	,	,
cord-275016-ij5yaqkx	145	26	although	although	IN
cord-275016-ij5yaqkx	145	27	the	the	DT
cord-275016-ij5yaqkx	145	28	degree	degree	NN
cord-275016-ij5yaqkx	145	29	of	of	IN
cord-275016-ij5yaqkx	145	30	inhibition	inhibition	NN
cord-275016-ij5yaqkx	145	31	varied	varied	JJ
cord-275016-ij5yaqkx	145	32	dependent	dependent	JJ
cord-275016-ij5yaqkx	145	33	on	on	IN
cord-275016-ij5yaqkx	145	34	the	the	DT
cord-275016-ij5yaqkx	145	35	sulfhydryl	sulfhydryl	NN
cord-275016-ij5yaqkx	145	36	reagents	reagent	NNS
cord-275016-ij5yaqkx	145	37	used	use	VBN
cord-275016-ij5yaqkx	145	38	.	.	.
cord-275016-ij5yaqkx	146	1	Small	small	JJ
cord-275016-ij5yaqkx	146	2	(	(	-LRB-
cord-275016-ij5yaqkx	146	3	Hg	Hg	NNP
cord-275016-ij5yaqkx	146	4	2	2	CD
cord-275016-ij5yaqkx	146	5	+	+	SYM
cord-275016-ij5yaqkx	146	6	,	,	,
cord-275016-ij5yaqkx	146	7	Zn	Zn	NNP
cord-275016-ij5yaqkx	146	8	2	2	CD
cord-275016-ij5yaqkx	146	9	+	+	CC
cord-275016-ij5yaqkx	146	10	,	,	,
cord-275016-ij5yaqkx	146	11	and	and	CC
cord-275016-ij5yaqkx	146	12	MMTS	MMTS	NNP
cord-275016-ij5yaqkx	146	13	)	)	-RRB-
cord-275016-ij5yaqkx	146	14	and	and	CC
cord-275016-ij5yaqkx	146	15	hydrophobic	hydrophobic	JJ
cord-275016-ij5yaqkx	146	16	(	(	-LRB-
cord-275016-ij5yaqkx	146	17	NEM	NEM	NNP
cord-275016-ij5yaqkx	146	18	and	and	CC
cord-275016-ij5yaqkx	146	19	NPM	NPM	NNP
cord-275016-ij5yaqkx	146	20	)	)	-RRB-
cord-275016-ij5yaqkx	146	21	reagents	reagent	NNS
cord-275016-ij5yaqkx	146	22	tended	tend	VBD
cord-275016-ij5yaqkx	146	23	to	to	TO
cord-275016-ij5yaqkx	146	24	inhibit	inhibit	VB
cord-275016-ij5yaqkx	146	25	the	the	DT
cord-275016-ij5yaqkx	146	26	activity	activity	NN
cord-275016-ij5yaqkx	146	27	more	more	RBR
cord-275016-ij5yaqkx	146	28	strongly	strongly	RB
cord-275016-ij5yaqkx	146	29	than	than	IN
cord-275016-ij5yaqkx	146	30	hydrophilic	hydrophilic	JJ
cord-275016-ij5yaqkx	146	31	reagents	reagent	NNS
cord-275016-ij5yaqkx	146	32	(	(	-LRB-
cord-275016-ij5yaqkx	146	33	PCMB	PCMB	NNP
cord-275016-ij5yaqkx	146	34	and	and	CC
cord-275016-ij5yaqkx	146	35	PCMBS	PCMBS	NNP
cord-275016-ij5yaqkx	146	36	)	)	-RRB-
cord-275016-ij5yaqkx	147	1	(	(	-LRB-
cord-275016-ij5yaqkx	147	2	Fig	Fig	NNP
cord-275016-ij5yaqkx	147	3	.	.	NNP
cord-275016-ij5yaqkx	147	4	5	5	CD
cord-275016-ij5yaqkx	147	5	)	)	-RRB-
cord-275016-ij5yaqkx	147	6	.	.	.
cord-275016-ij5yaqkx	148	1	This	this	DT
cord-275016-ij5yaqkx	148	2	tendency	tendency	NN
cord-275016-ij5yaqkx	148	3	may	may	MD
cord-275016-ij5yaqkx	148	4	reflect	reflect	VB
cord-275016-ij5yaqkx	148	5	the	the	DT
cord-275016-ij5yaqkx	148	6	environment	environment	NN
cord-275016-ij5yaqkx	148	7	of	of	IN
cord-275016-ij5yaqkx	148	8	the	the	DT
cord-275016-ij5yaqkx	148	9	active	active	JJ
cord-275016-ij5yaqkx	148	10	site	site	NN
cord-275016-ij5yaqkx	148	11	.	.	.
cord-275016-ij5yaqkx	149	1	The	the	DT
cord-275016-ij5yaqkx	149	2	present	present	JJ
cord-275016-ij5yaqkx	149	3	results	result	NNS
cord-275016-ij5yaqkx	149	4	provide	provide	VBP
cord-275016-ij5yaqkx	149	5	the	the	DT
cord-275016-ij5yaqkx	149	6	basic	basic	JJ
cord-275016-ij5yaqkx	149	7	biochemical	biochemical	JJ
cord-275016-ij5yaqkx	149	8	features	feature	NNS
cord-275016-ij5yaqkx	149	9	of	of	IN
cord-275016-ij5yaqkx	149	10	the	the	DT
cord-275016-ij5yaqkx	149	11	Norovirus	Norovirus	NNP
cord-275016-ij5yaqkx	149	12	3C	3c	NN
cord-275016-ij5yaqkx	149	13	-	-	HYPH
cord-275016-ij5yaqkx	149	14	like	like	JJ
cord-275016-ij5yaqkx	149	15	protease	protease	NN
cord-275016-ij5yaqkx	149	16	.	.	.
cord-275016-ij5yaqkx	150	1	Structure	structure	NN
cord-275016-ij5yaqkx	150	2	of	of	IN
cord-275016-ij5yaqkx	150	3	coronavirus	coronavirus	NN
cord-275016-ij5yaqkx	150	4	main	main	JJ
cord-275016-ij5yaqkx	150	5	proteinase	proteinase	NN
cord-275016-ij5yaqkx	150	6	reveals	reveal	VBZ
cord-275016-ij5yaqkx	150	7	combination	combination	NN
cord-275016-ij5yaqkx	150	8	of	of	IN
cord-275016-ij5yaqkx	150	9	a	a	DT
cord-275016-ij5yaqkx	150	10	chymotrypsin	chymotrypsin	NN
cord-275016-ij5yaqkx	150	11	fold	fold	JJ
cord-275016-ij5yaqkx	150	12	with	with	IN
cord-275016-ij5yaqkx	150	13	an	an	DT
cord-275016-ij5yaqkx	150	14	extra	extra	JJ
cord-275016-ij5yaqkx	150	15	alpha	alpha	JJ
cord-275016-ij5yaqkx	150	16	-	-	HYPH
cord-275016-ij5yaqkx	150	17	helical	helical	JJ
cord-275016-ij5yaqkx	150	18	domain	domain	NN
cord-275016-ij5yaqkx	150	19	Coronavirus	Coronavirus	NNP
cord-275016-ij5yaqkx	150	20	main	main	JJ
cord-275016-ij5yaqkx	150	21	proteinase	proteinase	NN
cord-275016-ij5yaqkx	150	22	(	(	-LRB-
cord-275016-ij5yaqkx	150	23	3CLpro	3clpro	CD
cord-275016-ij5yaqkx	150	24	)	)	-RRB-
cord-275016-ij5yaqkx	150	25	structure	structure	NN
cord-275016-ij5yaqkx	150	26	:	:	:
cord-275016-ij5yaqkx	150	27	basis	basis	NN
cord-275016-ij5yaqkx	150	28	for	for	IN
cord-275016-ij5yaqkx	150	29	design	design	NN
cord-275016-ij5yaqkx	150	30	of	of	IN
cord-275016-ij5yaqkx	150	31	anti	anti	JJ
cord-275016-ij5yaqkx	150	32	-	-	JJ
cord-275016-ij5yaqkx	150	33	SARS	SARS	NNP
cord-275016-ij5yaqkx	150	34	drugs	drug	NNS
cord-275016-ij5yaqkx	150	35	Structure	Structure	NNP
cord-275016-ij5yaqkx	150	36	of	of	IN
cord-275016-ij5yaqkx	150	37	arterivirus	arterivirus	NNP
cord-275016-ij5yaqkx	150	38	nsp4	nsp4	NNP
cord-275016-ij5yaqkx	150	39	.	.	.
cord-275016-ij5yaqkx	151	1	The	the	DT
cord-275016-ij5yaqkx	151	2	smallest	small	JJS
cord-275016-ij5yaqkx	151	3	chymotrypsinlike	chymotrypsinlike	JJ
cord-275016-ij5yaqkx	151	4	proteinase	proteinase	NN
cord-275016-ij5yaqkx	151	5	with	with	IN
cord-275016-ij5yaqkx	151	6	an	an	DT
cord-275016-ij5yaqkx	151	7	alpha	alpha	NN
cord-275016-ij5yaqkx	151	8	/	/	SYM
cord-275016-ij5yaqkx	151	9	beta	beta	NN
cord-275016-ij5yaqkx	151	10	C	C	NNP
cord-275016-ij5yaqkx	151	11	-	-	HYPH
cord-275016-ij5yaqkx	151	12	terminal	terminal	NN
cord-275016-ij5yaqkx	151	13	extension	extension	NN
cord-275016-ij5yaqkx	151	14	and	and	CC
cord-275016-ij5yaqkx	151	15	alternate	alternate	JJ
cord-275016-ij5yaqkx	151	16	conformations	conformation	NNS
cord-275016-ij5yaqkx	151	17	of	of	IN
cord-275016-ij5yaqkx	151	18	the	the	DT
cord-275016-ij5yaqkx	151	19	oxyanion	oxyanion	NN
cord-275016-ij5yaqkx	151	20	hole	hole	NN
cord-275016-ij5yaqkx	151	21	Purification	Purification	NNP
cord-275016-ij5yaqkx	151	22	,	,	,
cord-275016-ij5yaqkx	151	23	properties	property	NNS
cord-275016-ij5yaqkx	151	24	,	,	,
cord-275016-ij5yaqkx	151	25	and	and	CC
cord-275016-ij5yaqkx	151	26	mutagenesis	mutagenesis	NN
cord-275016-ij5yaqkx	151	27	of	of	IN
cord-275016-ij5yaqkx	151	28	poliovirus	poliovirus	NN
cord-275016-ij5yaqkx	151	29	3C	3C	NNP
cord-275016-ij5yaqkx	151	30	protease	protease	NN
cord-275016-ij5yaqkx	152	1	In	in	IN
cord-275016-ij5yaqkx	152	2	vitro	vitro	FW
cord-275016-ij5yaqkx	152	3	proteolytic	proteolytic	JJ
cord-275016-ij5yaqkx	152	4	processing	processing	NN
cord-275016-ij5yaqkx	152	5	of	of	IN
cord-275016-ij5yaqkx	152	6	the	the	DT
cord-275016-ij5yaqkx	152	7	MD145	MD145	NNP
cord-275016-ij5yaqkx	152	8	norovirus	norovirus	NN
cord-275016-ij5yaqkx	152	9	ORF1	ORF1	NNP
cord-275016-ij5yaqkx	152	10	non	non	JJ
cord-275016-ij5yaqkx	152	11	-	-	JJ
cord-275016-ij5yaqkx	152	12	structural	structural	JJ
cord-275016-ij5yaqkx	152	13	polyprotein	polyprotein	NN
cord-275016-ij5yaqkx	152	14	yields	yield	VBZ
cord-275016-ij5yaqkx	152	15	stable	stable	JJ
cord-275016-ij5yaqkx	152	16	precursors	precursor	NNS
cord-275016-ij5yaqkx	152	17	and	and	CC
cord-275016-ij5yaqkx	152	18	products	product	NNS
cord-275016-ij5yaqkx	152	19	similar	similar	JJ
cord-275016-ij5yaqkx	152	20	to	to	IN
cord-275016-ij5yaqkx	152	21	those	those	DT
cord-275016-ij5yaqkx	152	22	detected	detect	VBN
cord-275016-ij5yaqkx	152	23	in	in	IN
cord-275016-ij5yaqkx	152	24	calicivirus	calicivirus	NNP
cord-275016-ij5yaqkx	152	25	-	-	HYPH
cord-275016-ij5yaqkx	152	26	infected	infect	VBN
cord-275016-ij5yaqkx	152	27	cells	cell	NNS
cord-275016-ij5yaqkx	153	1	The	the	DT
cord-275016-ij5yaqkx	153	2	refined	refined	JJ
cord-275016-ij5yaqkx	153	3	crystal	crystal	NN
cord-275016-ij5yaqkx	153	4	structure	structure	NN
cord-275016-ij5yaqkx	153	5	of	of	IN
cord-275016-ij5yaqkx	153	6	the	the	DT
cord-275016-ij5yaqkx	153	7	3C	3C	NNP
cord-275016-ij5yaqkx	153	8	gene	gene	NN
cord-275016-ij5yaqkx	153	9	product	product	NN
cord-275016-ij5yaqkx	153	10	from	from	IN
cord-275016-ij5yaqkx	153	11	hepatitis	hepatitis	NNP
cord-275016-ij5yaqkx	153	12	A	A	NNP
cord-275016-ij5yaqkx	153	13	virus	virus	NN
cord-275016-ij5yaqkx	153	14	:	:	:
cord-275016-ij5yaqkx	153	15	specific	specific	JJ
cord-275016-ij5yaqkx	153	16	proteinase	proteinase	NN
cord-275016-ij5yaqkx	153	17	activity	activity	NN
cord-275016-ij5yaqkx	153	18	and	and	CC
cord-275016-ij5yaqkx	153	19	RNA	RNA	NNP
cord-275016-ij5yaqkx	153	20	recognition	recognition	NN
cord-275016-ij5yaqkx	153	21	Identification	identification	NN
cord-275016-ij5yaqkx	153	22	and	and	CC
cord-275016-ij5yaqkx	153	23	characterization	characterization	NN
cord-275016-ij5yaqkx	153	24	of	of	IN
cord-275016-ij5yaqkx	153	25	a	a	DT
cord-275016-ij5yaqkx	153	26	3C	3c	NN
cord-275016-ij5yaqkx	153	27	-	-	HYPH
cord-275016-ij5yaqkx	153	28	like	like	JJ
cord-275016-ij5yaqkx	153	29	protease	protease	NN
cord-275016-ij5yaqkx	153	30	from	from	IN
cord-275016-ij5yaqkx	153	31	rabbit	rabbit	NN
cord-275016-ij5yaqkx	153	32	hemorrhagic	hemorrhagic	JJ
cord-275016-ij5yaqkx	153	33	disease	disease	NN
cord-275016-ij5yaqkx	153	34	virus	virus	NN
cord-275016-ij5yaqkx	153	35	,	,	,
cord-275016-ij5yaqkx	153	36	a	a	DT
cord-275016-ij5yaqkx	153	37	calicivirus	calicivirus	NN
cord-275016-ij5yaqkx	153	38	A	a	DT
cord-275016-ij5yaqkx	153	39	rapid	rapid	JJ
cord-275016-ij5yaqkx	153	40	and	and	CC
cord-275016-ij5yaqkx	153	41	sensitive	sensitive	JJ
cord-275016-ij5yaqkx	153	42	method	method	NN
cord-275016-ij5yaqkx	153	43	for	for	IN
cord-275016-ij5yaqkx	153	44	the	the	DT
cord-275016-ij5yaqkx	153	45	quantitation	quantitation	NN
cord-275016-ij5yaqkx	153	46	of	of	IN
cord-275016-ij5yaqkx	153	47	microgram	microgram	NN
cord-275016-ij5yaqkx	153	48	quantities	quantity	NNS
cord-275016-ij5yaqkx	153	49	of	of	IN
cord-275016-ij5yaqkx	153	50	protein	protein	NN
cord-275016-ij5yaqkx	153	51	utilizing	utilize	VBG
cord-275016-ij5yaqkx	153	52	the	the	DT
cord-275016-ij5yaqkx	153	53	principle	principle	NN
cord-275016-ij5yaqkx	153	54	of	of	IN
cord-275016-ij5yaqkx	153	55	proteindye	proteindye	NN
cord-275016-ij5yaqkx	153	56	binding	bind	VBG
cord-275016-ij5yaqkx	153	57	Site	site	NN
cord-275016-ij5yaqkx	153	58	-	-	HYPH
cord-275016-ij5yaqkx	153	59	directed	direct	VBN
cord-275016-ij5yaqkx	153	60	mutagenesis	mutagenesis	NN
cord-275016-ij5yaqkx	153	61	suggests	suggest	VBZ
cord-275016-ij5yaqkx	153	62	close	close	JJ
cord-275016-ij5yaqkx	153	63	functional	functional	JJ
cord-275016-ij5yaqkx	153	64	relationship	relationship	NN
cord-275016-ij5yaqkx	153	65	between	between	IN
cord-275016-ij5yaqkx	153	66	a	a	DT
cord-275016-ij5yaqkx	153	67	human	human	JJ
cord-275016-ij5yaqkx	153	68	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	153	69	3C	3C	NNP
cord-275016-ij5yaqkx	153	70	cysteine	cysteine	NN
cord-275016-ij5yaqkx	153	71	protease	protease	NN
cord-275016-ij5yaqkx	153	72	and	and	CC
cord-275016-ij5yaqkx	153	73	cellular	cellular	JJ
cord-275016-ij5yaqkx	153	74	trypsin	trypsin	NN
cord-275016-ij5yaqkx	153	75	-	-	HYPH
cord-275016-ij5yaqkx	153	76	like	like	JJ
cord-275016-ij5yaqkx	153	77	serine	serine	NN
cord-275016-ij5yaqkx	153	78	proteases	protease	NNS
cord-275016-ij5yaqkx	153	79	Expression	expression	NN
cord-275016-ij5yaqkx	153	80	and	and	CC
cord-275016-ij5yaqkx	153	81	partial	partial	JJ
cord-275016-ij5yaqkx	153	82	purification	purification	NN
cord-275016-ij5yaqkx	153	83	of	of	IN
cord-275016-ij5yaqkx	153	84	recombinant	recombinant	JJ
cord-275016-ij5yaqkx	153	85	tomato	tomato	NNP
cord-275016-ij5yaqkx	153	86	ringspot	ringspot	NNP
cord-275016-ij5yaqkx	153	87	nepovirus	nepovirus	NNP
cord-275016-ij5yaqkx	153	88	3C	3C	NNP
cord-275016-ij5yaqkx	153	89	-	-	HYPH
cord-275016-ij5yaqkx	153	90	like	like	JJ
cord-275016-ij5yaqkx	153	91	proteinase	proteinase	NN
cord-275016-ij5yaqkx	153	92	:	:	:
cord-275016-ij5yaqkx	153	93	comparison	comparison	NN
cord-275016-ij5yaqkx	153	94	of	of	IN
cord-275016-ij5yaqkx	153	95	the	the	DT
cord-275016-ij5yaqkx	153	96	activity	activity	NN
cord-275016-ij5yaqkx	153	97	of	of	IN
cord-275016-ij5yaqkx	153	98	the	the	DT
cord-275016-ij5yaqkx	153	99	mature	mature	JJ
cord-275016-ij5yaqkx	153	100	proteinase	proteinase	NN
cord-275016-ij5yaqkx	153	101	and	and	CC
cord-275016-ij5yaqkx	153	102	the	the	DT
cord-275016-ij5yaqkx	153	103	VPg	vpg	NN
cord-275016-ij5yaqkx	153	104	-	-	HYPH
cord-275016-ij5yaqkx	153	105	proteinase	proteinase	NN
cord-275016-ij5yaqkx	153	106	precursor	precursor	NN
cord-275016-ij5yaqkx	153	107	Human	Human	NNP
cord-275016-ij5yaqkx	153	108	enteric	enteric	JJ
cord-275016-ij5yaqkx	153	109	RNA	RNA	NNP
cord-275016-ij5yaqkx	153	110	viruses	virus	NNS
cord-275016-ij5yaqkx	153	111	:	:	:
cord-275016-ij5yaqkx	153	112	caliciviruses	caliciviruse	NNS
cord-275016-ij5yaqkx	153	113	and	and	CC
cord-275016-ij5yaqkx	153	114	astroviruses	astroviruses	NNPS
cord-275016-ij5yaqkx	153	115	Expression	expression	NN
cord-275016-ij5yaqkx	153	116	and	and	CC
cord-275016-ij5yaqkx	153	117	purification	purification	NN
cord-275016-ij5yaqkx	153	118	of	of	IN
cord-275016-ij5yaqkx	153	119	recombinant	recombinant	JJ
cord-275016-ij5yaqkx	153	120	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	153	121	14	14	CD
cord-275016-ij5yaqkx	153	122	3CD	3cd	CD
cord-275016-ij5yaqkx	153	123	proteinase	proteinase	NN
cord-275016-ij5yaqkx	153	124	and	and	CC
cord-275016-ij5yaqkx	153	125	its	-PRON-	PRP$
cord-275016-ij5yaqkx	153	126	comparison	comparison	NN
cord-275016-ij5yaqkx	153	127	to	to	IN
cord-275016-ij5yaqkx	153	128	the	the	DT
cord-275016-ij5yaqkx	153	129	3C	3C	NNP
cord-275016-ij5yaqkx	153	130	proteinase	proteinase	NNP
cord-275016-ij5yaqkx	153	131	Expression	Expression	NNP
cord-275016-ij5yaqkx	153	132	of	of	IN
cord-275016-ij5yaqkx	153	133	virus	virus	NN
cord-275016-ij5yaqkx	153	134	-	-	HYPH
cord-275016-ij5yaqkx	153	135	encoded	encode	VBN
cord-275016-ij5yaqkx	153	136	proteinases	proteinase	NNS
cord-275016-ij5yaqkx	153	137	:	:	:
cord-275016-ij5yaqkx	153	138	functional	functional	JJ
cord-275016-ij5yaqkx	153	139	and	and	CC
cord-275016-ij5yaqkx	153	140	structural	structural	JJ
cord-275016-ij5yaqkx	153	141	similarities	similarity	NNS
cord-275016-ij5yaqkx	153	142	with	with	IN
cord-275016-ij5yaqkx	153	143	cellular	cellular	JJ
cord-275016-ij5yaqkx	153	144	enzymes	enzyme	NNS
cord-275016-ij5yaqkx	153	145	Norwalk	Norwalk	NNP
cord-275016-ij5yaqkx	153	146	and	and	CC
cord-275016-ij5yaqkx	153	147	related	related	JJ
cord-275016-ij5yaqkx	153	148	diarrhea	diarrhea	NN
cord-275016-ij5yaqkx	153	149	viruses	virus	NNS
cord-275016-ij5yaqkx	153	150	Cysteine	cysteine	NN
cord-275016-ij5yaqkx	153	151	proteases	protease	NNS
cord-275016-ij5yaqkx	153	152	of	of	IN
cord-275016-ij5yaqkx	153	153	positive	positive	JJ
cord-275016-ij5yaqkx	153	154	strand	strand	NN
cord-275016-ij5yaqkx	153	155	RNA	RNA	NNP
cord-275016-ij5yaqkx	153	156	viruses	virus	NNS
cord-275016-ij5yaqkx	153	157	and	and	CC
cord-275016-ij5yaqkx	153	158	chymotrypsinlike	chymotrypsinlike	JJ
cord-275016-ij5yaqkx	153	159	serine	serine	NN
cord-275016-ij5yaqkx	153	160	proteases	protease	NNS
cord-275016-ij5yaqkx	153	161	A	a	DT
cord-275016-ij5yaqkx	153	162	distinct	distinct	JJ
cord-275016-ij5yaqkx	153	163	protein	protein	NN
cord-275016-ij5yaqkx	153	164	superfamily	superfamily	NN
cord-275016-ij5yaqkx	153	165	with	with	IN
cord-275016-ij5yaqkx	153	166	a	a	DT
cord-275016-ij5yaqkx	153	167	common	common	JJ
cord-275016-ij5yaqkx	153	168	structural	structural	NN
cord-275016-ij5yaqkx	153	169	fold	fold	NN
cord-275016-ij5yaqkx	153	170	Identification	identification	NN
cord-275016-ij5yaqkx	153	171	of	of	IN
cord-275016-ij5yaqkx	153	172	active	active	JJ
cord-275016-ij5yaqkx	153	173	-	-	HYPH
cord-275016-ij5yaqkx	153	174	site	site	NN
cord-275016-ij5yaqkx	153	175	residues	residue	NNS
cord-275016-ij5yaqkx	153	176	in	in	IN
cord-275016-ij5yaqkx	153	177	protease	protease	NN
cord-275016-ij5yaqkx	153	178	3C	3c	NN
cord-275016-ij5yaqkx	153	179	of	of	IN
cord-275016-ij5yaqkx	153	180	hepatitis	hepatitis	NNP
cord-275016-ij5yaqkx	153	181	A	A	NNP
cord-275016-ij5yaqkx	153	182	virus	virus	NN
cord-275016-ij5yaqkx	153	183	by	by	IN
cord-275016-ij5yaqkx	153	184	site	site	NN
cord-275016-ij5yaqkx	153	185	-	-	HYPH
cord-275016-ij5yaqkx	153	186	directed	direct	VBN
cord-275016-ij5yaqkx	153	187	mutagenesis	mutagenesis	NN
cord-275016-ij5yaqkx	153	188	Site	site	NN
cord-275016-ij5yaqkx	153	189	-	-	HYPH
cord-275016-ij5yaqkx	153	190	directed	direct	VBN
cord-275016-ij5yaqkx	153	191	mutagenesis	mutagenesis	NN
cord-275016-ij5yaqkx	153	192	of	of	IN
cord-275016-ij5yaqkx	153	193	the	the	DT
cord-275016-ij5yaqkx	153	194	putative	putative	JJ
cord-275016-ij5yaqkx	153	195	catalytic	catalytic	JJ
cord-275016-ij5yaqkx	153	196	triad	triad	NN
cord-275016-ij5yaqkx	153	197	of	of	IN
cord-275016-ij5yaqkx	153	198	poliovirus	poliovirus	NNP
cord-275016-ij5yaqkx	153	199	3C	3C	NNP
cord-275016-ij5yaqkx	153	200	proteinase	proteinase	NNP
cord-275016-ij5yaqkx	153	201	Structure	Structure	NNP
cord-275016-ij5yaqkx	153	202	of	of	IN
cord-275016-ij5yaqkx	153	203	human	human	JJ
cord-275016-ij5yaqkx	153	204	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	153	205	3C	3C	NNP
cord-275016-ij5yaqkx	153	206	protease	protease	NNP
cord-275016-ij5yaqkx	153	207	reveals	reveal	VBZ
cord-275016-ij5yaqkx	153	208	a	a	DT
cord-275016-ij5yaqkx	153	209	trypsin	trypsin	NN
cord-275016-ij5yaqkx	153	210	-	-	HYPH
cord-275016-ij5yaqkx	153	211	like	like	JJ
cord-275016-ij5yaqkx	153	212	polypeptide	polypeptide	NN
cord-275016-ij5yaqkx	153	213	fold	fold	VBG
cord-275016-ij5yaqkx	153	214	,	,	,
cord-275016-ij5yaqkx	153	215	RNA	rna	NN
cord-275016-ij5yaqkx	153	216	-	-	HYPH
cord-275016-ij5yaqkx	153	217	binding	bind	VBG
cord-275016-ij5yaqkx	153	218	site	site	NN
cord-275016-ij5yaqkx	153	219	,	,	,
cord-275016-ij5yaqkx	153	220	and	and	CC
cord-275016-ij5yaqkx	153	221	means	mean	VBZ
cord-275016-ij5yaqkx	153	222	for	for	IN
cord-275016-ij5yaqkx	153	223	cleaving	cleave	VBG
cord-275016-ij5yaqkx	153	224	precursor	precursor	NN
cord-275016-ij5yaqkx	153	225	polyprotein	polyprotein	NN
cord-275016-ij5yaqkx	154	1	Recent	recent	JJ
cord-275016-ij5yaqkx	154	2	advances	advance	NNS
cord-275016-ij5yaqkx	154	3	in	in	IN
cord-275016-ij5yaqkx	154	4	the	the	DT
cord-275016-ij5yaqkx	154	5	treatment	treatment	NN
cord-275016-ij5yaqkx	154	6	of	of	IN
cord-275016-ij5yaqkx	154	7	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	154	8	infections	infection	NNS
cord-275016-ij5yaqkx	154	9	Active	active	JJ
cord-275016-ij5yaqkx	154	10	efflux	efflux	NN
cord-275016-ij5yaqkx	154	11	of	of	IN
cord-275016-ij5yaqkx	154	12	tetracycline	tetracycline	NN
cord-275016-ij5yaqkx	154	13	encoded	encode	VBN
cord-275016-ij5yaqkx	154	14	by	by	IN
cord-275016-ij5yaqkx	154	15	four	four	CD
cord-275016-ij5yaqkx	154	16	genetically	genetically	RB
cord-275016-ij5yaqkx	154	17	different	different	JJ
cord-275016-ij5yaqkx	154	18	tetracycline	tetracycline	NN
cord-275016-ij5yaqkx	154	19	resistance	resistance	NN
cord-275016-ij5yaqkx	154	20	determinants	determinant	NNS
cord-275016-ij5yaqkx	154	21	in	in	IN
cord-275016-ij5yaqkx	154	22	Escherichia	Escherichia	NNP
cord-275016-ij5yaqkx	154	23	coli	coli	NNS
cord-275016-ij5yaqkx	155	1	Refined	refined	JJ
cord-275016-ij5yaqkx	155	2	X	x	NN
cord-275016-ij5yaqkx	155	3	-	-	NN
cord-275016-ij5yaqkx	155	4	ray	ray	NN
cord-275016-ij5yaqkx	155	5	crystallographic	crystallographic	JJ
cord-275016-ij5yaqkx	155	6	structure	structure	NN
cord-275016-ij5yaqkx	155	7	of	of	IN
cord-275016-ij5yaqkx	155	8	the	the	DT
cord-275016-ij5yaqkx	155	9	poliovirus	poliovirus	NN
cord-275016-ij5yaqkx	155	10	3C	3C	NNP
cord-275016-ij5yaqkx	155	11	gene	gene	NN
cord-275016-ij5yaqkx	155	12	product	product	NN
cord-275016-ij5yaqkx	155	13	Structural	structural	JJ
cord-275016-ij5yaqkx	155	14	basis	basis	NN
cord-275016-ij5yaqkx	155	15	for	for	IN
cord-275016-ij5yaqkx	155	16	the	the	DT
cord-275016-ij5yaqkx	155	17	substrate	substrate	JJ
cord-275016-ij5yaqkx	155	18	specificity	specificity	NN
cord-275016-ij5yaqkx	155	19	of	of	IN
cord-275016-ij5yaqkx	155	20	tobacco	tobacco	NN
cord-275016-ij5yaqkx	155	21	etch	etch	NN
cord-275016-ij5yaqkx	155	22	virus	virus	NN
cord-275016-ij5yaqkx	155	23	protease	protease	NN
cord-275016-ij5yaqkx	155	24	Complete	complete	JJ
cord-275016-ij5yaqkx	155	25	nucleotide	nucleotide	JJ
cord-275016-ij5yaqkx	155	26	sequence	sequence	NN
cord-275016-ij5yaqkx	155	27	of	of	IN
cord-275016-ij5yaqkx	155	28	the	the	DT
cord-275016-ij5yaqkx	155	29	chiba	chiba	NNP
cord-275016-ij5yaqkx	155	30	virus	virus	NN
cord-275016-ij5yaqkx	155	31	genome	genome	NN
cord-275016-ij5yaqkx	155	32	and	and	CC
cord-275016-ij5yaqkx	155	33	functional	functional	JJ
cord-275016-ij5yaqkx	155	34	expression	expression	NN
cord-275016-ij5yaqkx	155	35	of	of	IN
cord-275016-ij5yaqkx	155	36	the	the	DT
cord-275016-ij5yaqkx	155	37	3C	3c	NN
cord-275016-ij5yaqkx	155	38	-	-	HYPH
cord-275016-ij5yaqkx	155	39	like	like	JJ
cord-275016-ij5yaqkx	155	40	protease	protease	NN
cord-275016-ij5yaqkx	155	41	in	in	IN
cord-275016-ij5yaqkx	155	42	Escherichia	Escherichia	NNP
cord-275016-ij5yaqkx	155	43	coli	coli	VBZ
cord-275016-ij5yaqkx	155	44	Identification	identification	NN
cord-275016-ij5yaqkx	155	45	of	of	IN
cord-275016-ij5yaqkx	155	46	active	active	JJ
cord-275016-ij5yaqkx	155	47	-	-	HYPH
cord-275016-ij5yaqkx	155	48	site	site	NN
cord-275016-ij5yaqkx	155	49	amino	amino	NN
cord-275016-ij5yaqkx	155	50	acid	acid	NN
cord-275016-ij5yaqkx	155	51	residues	residue	NNS
cord-275016-ij5yaqkx	155	52	in	in	IN
cord-275016-ij5yaqkx	155	53	the	the	DT
cord-275016-ij5yaqkx	155	54	Chiba	Chiba	NNP
cord-275016-ij5yaqkx	155	55	virus	virus	NN
cord-275016-ij5yaqkx	155	56	3C	3c	NN
cord-275016-ij5yaqkx	155	57	-	-	HYPH
cord-275016-ij5yaqkx	155	58	like	like	JJ
cord-275016-ij5yaqkx	155	59	protease	protease	NN
cord-275016-ij5yaqkx	156	1	The	the	DT
cord-275016-ij5yaqkx	156	2	treatment	treatment	NN
cord-275016-ij5yaqkx	156	3	of	of	IN
cord-275016-ij5yaqkx	156	4	rhinovirus	rhinovirus	NN
cord-275016-ij5yaqkx	156	5	infections	infection	NNS
cord-275016-ij5yaqkx	156	6	:	:	:
cord-275016-ij5yaqkx	156	7	progress	progress	NN
cord-275016-ij5yaqkx	156	8	and	and	CC
cord-275016-ij5yaqkx	156	9	potential	potential	JJ
cord-275016-ij5yaqkx	156	10	The	the	DT
cord-275016-ij5yaqkx	156	11	crystal	crystal	NN
cord-275016-ij5yaqkx	156	12	structures	structure	NNS
cord-275016-ij5yaqkx	156	13	of	of	IN
cord-275016-ij5yaqkx	156	14	severe	severe	JJ
cord-275016-ij5yaqkx	156	15	acute	acute	JJ
cord-275016-ij5yaqkx	156	16	respiratory	respiratory	JJ
cord-275016-ij5yaqkx	156	17	syndrome	syndrome	NN
cord-275016-ij5yaqkx	156	18	virus	virus	NN
cord-275016-ij5yaqkx	156	19	main	main	JJ
cord-275016-ij5yaqkx	156	20	protease	protease	NN
cord-275016-ij5yaqkx	156	21	and	and	CC
cord-275016-ij5yaqkx	156	22	its	-PRON-	PRP$
cord-275016-ij5yaqkx	156	23	complex	complex	NN
cord-275016-ij5yaqkx	156	24	with	with	IN
cord-275016-ij5yaqkx	156	25	an	an	DT
cord-275016-ij5yaqkx	156	26	inhibitor	inhibitor	NN
cord-275016-ij5yaqkx	156	27	Biosynthesis	Biosynthesis	NNP
cord-275016-ij5yaqkx	156	28	,	,	,
cord-275016-ij5yaqkx	156	29	purification	purification	NN
cord-275016-ij5yaqkx	156	30	,	,	,
cord-275016-ij5yaqkx	156	31	and	and	CC
cord-275016-ij5yaqkx	156	32	characterization	characterization	NN
cord-275016-ij5yaqkx	156	33	of	of	IN
cord-275016-ij5yaqkx	156	34	the	the	DT
cord-275016-ij5yaqkx	156	35	human	human	JJ
cord-275016-ij5yaqkx	156	36	coronavirus	coronavirus	NN
cord-275016-ij5yaqkx	157	1	229E	229e	CD
cord-275016-ij5yaqkx	157	2	3C	3c	NN
cord-275016-ij5yaqkx	157	3	-	-	HYPH
cord-275016-ij5yaqkx	157	4	like	like	JJ
cord-275016-ij5yaqkx	157	5	proteinase	proteinase	NN
cord-275016-ij5yaqkx	158	1	We	-PRON-	PRP
cord-275016-ij5yaqkx	158	2	thank	thank	VBP
cord-275016-ij5yaqkx	158	3	T.	T.	NNP
cord-275016-ij5yaqkx	158	4	Mizoguchi	Mizoguchi	NNP
cord-275016-ij5yaqkx	158	5	for	for	IN
cord-275016-ij5yaqkx	158	6	her	-PRON-	PRP$
cord-275016-ij5yaqkx	158	7	secretarial	secretarial	JJ
cord-275016-ij5yaqkx	158	8	work	work	NN
cord-275016-ij5yaqkx	158	9	.	.	.
cord-275016-ij5yaqkx	159	1	This	this	DT
cord-275016-ij5yaqkx	159	2	work	work	NN
cord-275016-ij5yaqkx	159	3	was	be	VBD
cord-275016-ij5yaqkx	159	4	supported	support	VBN
cord-275016-ij5yaqkx	159	5	in	in	IN
cord-275016-ij5yaqkx	159	6	part	part	NN
cord-275016-ij5yaqkx	159	7	by	by	IN
cord-275016-ij5yaqkx	159	8	a	a	DT
cord-275016-ij5yaqkx	159	9	grant	grant	NN
cord-275016-ij5yaqkx	159	10	for	for	IN
cord-275016-ij5yaqkx	159	11	Research	Research	NNP
cord-275016-ij5yaqkx	159	12	on	on	IN
cord-275016-ij5yaqkx	159	13	Emerging	Emerging	NNP
cord-275016-ij5yaqkx	159	14	and	and	CC
cord-275016-ij5yaqkx	159	15	Re	Re	NNS
cord-275016-ij5yaqkx	159	16	-	-	NN
cord-275016-ij5yaqkx	159	17	emerging	emerge	VBG
cord-275016-ij5yaqkx	159	18	Infectious	Infectious	NNP
cord-275016-ij5yaqkx	159	19	Diseases	Diseases	NNPS
cord-275016-ij5yaqkx	159	20	from	from	IN
cord-275016-ij5yaqkx	159	21	the	the	DT
cord-275016-ij5yaqkx	159	22	Ministry	Ministry	NNP
cord-275016-ij5yaqkx	159	23	of	of	IN
cord-275016-ij5yaqkx	159	24	Health	Health	NNP
cord-275016-ij5yaqkx	159	25	,	,	,
cord-275016-ij5yaqkx	159	26	Labor	Labor	NNP
cord-275016-ij5yaqkx	159	27	,	,	,
cord-275016-ij5yaqkx	159	28	and	and	CC
cord-275016-ij5yaqkx	159	29	Welfare	Welfare	NNP
cord-275016-ij5yaqkx	159	30	.	.	.