id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003646-kjkuet78	López-Camacho, César	Assessment of Immunogenicity and Neutralisation Efficacy of Viral-Vectored Vaccines Against Chikungunya Virus	2019-04-03		.txt	text/plain	6359	314	49	We have designed viral-vectored vaccines to express the structural proteins of CHIKV, using the replication-deficient chimpanzee adenoviral platform, ChAdOx1. Our vaccines induce high frequencies of anti-chikungunya specific T-cell responses as well as high titres of anti-CHIKV E2 antibodies with high capacity for in vitro neutralisation. In mice, ChAdOx1 vaccines candidates expressing sCHIKV antigens are able to induce strong humoral and cellular responses upon a single and non-adjuvanted immunisation approach. Finally, whilst durability of humoral responses was achieved upon a single immunisation, MVA vaccines expressing sCHIKV were produced to be used as an alternative heterologous booster regime (ChAdOx1/MVA), in order to increase neutralising antibody titres. In addition, a western blot was performed using a monoclonal antibody against CHIKV E1 (Figure 3d ); we detected a specific band in the positive control E1 protein (lane 9), as well as a 55 kDa band in all the supernatant fractions from both ChAdOx1 sCHIKV-and ChAdOx1 sCHIKV ∆C-transduced cells.	./cache/cord-003646-kjkuet78.txt	./txt/cord-003646-kjkuet78.txt