id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325743-5ujiscdt	Kitajima, Ken	Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis	2015-05-28		.txt	text/plain	7597	409	45	Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn), O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. In this chapter we summarize current chemical and immunochemical methods to detect modified Sia species, with a special focus on O-acetylated Neu5Ac, Neu5Gc, Kdn, O-sulfated Sia, as well as diSia, oligoSia, and polySia. Because other chapters in this volume specifically focus on O-acetylated Neu5Ac [7] , Neu5Gc [8] , and polySia [9] , we restrict ourselves in this chapter to a brief introduction of the different Sia species. To observe and quantify the internal sialic acids of α2!8-linked di-, oligo-, and polySia-containing glycoconjugates, sensitive chemical methods were developed with highly sensitive fluorescent labeling reagents (DMB) as described above [69] .	./cache/cord-325743-5ujiscdt.txt	./txt/cord-325743-5ujiscdt.txt