id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005337-3f6pwyy3	Yoon, Hyun Kyung	Recent development of highly sensitive protease assay methods: Signal amplification through enzyme cascades	2013-01-04		.txt	text/plain	4060	197	43	title: Recent development of highly sensitive protease assay methods: Signal amplification through enzyme cascades Various methods have been developed to analyze protease activity, among which, protease assays based on resonance energy transfer are currently used most widely. In this review, we briefly summarize the protease assay methods based on resonance energy transfer, and then elaborate the efforts to develop sensitive protease assays through signal amplification by using enzyme cascades. FRET methods have been widely used to assay protease activities because of its advantages, especially coming from the fact the signal is based on fluorescence [17, 18] . For instance, a highthroughput FRET-based method for assaying OmpT endopeptidase activity was used for engineering the substrate specificity of the enzyme [23, 24] . Detection and characterization of SUMO protease activity using a sensitive enzyme-based reporter assay	./cache/cord-005337-3f6pwyy3.txt	./txt/cord-005337-3f6pwyy3.txt