id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007682-01iom9al	Harrigan, Jeanine	Monitoring Target Engagement of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future	2016-09-10		.txt	text/plain	5506	259	39	An alternative to classical activity probes containing a full ubiquitin or ubiquitin-like polypeptide is based on the use of small molecule inhibitors to label the catalytic site of desumoylating enzymes (sentrin-specifi c proteases, SENPs ). Indeed, the complexity of the recognition site of DUBs, which requires the binding of full-length ubiquitin in the catalytic site as well as the challenges in the characterization of potent and selective DUB inhibitors , has hindered production of ABPs for DUBs. However, following on from the ground-breaking evolution of cell-permeable and in vivo-compatible activity-based imaging probes developed for other proteases such as caspases or cathepsins [ 69 , 70 ] , the next generation of probes for DUBs will certainly be agents that enable direct visualization and quantifi cation of DUB activity in vivo.	./cache/cord-007682-01iom9al.txt	./txt/cord-007682-01iom9al.txt