id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312954-pnmycagi	Tola, Monday	Molecular detection of drug resistant polymorphisms in Plasmodium falciparum isolates from Southwest, Nigeria	2020-10-27		.txt	text/plain	3272	179	50	This study describes mutations in Plasmodium falciparum genes associated with drug resistance in malaria; Pfk13, Pfmdr1, PfATPase6 and Pfcrt in isolates obtained from 83 symptomatic malaria patients collected in August 2014, aged 1–61 years old from South-west Nigeria. Drug resistance of malaria parasites to previously efficacious first line chemotherapies, chloroquine (CQ) and sulfadoxine-pyrimethamine (SP), in sub-Saharan Africa (sSA), led to replacement with artemisinin combination therapies (ACT), and complete removal of CQ [1, 2] . This study describes the molecular prevalence of mutations in the drug resistant genes Pfk13, Pfmdr1, PfATPase6 and Pfcrt from P. Alleles of Pfk13 propeller domain polymorphisms (Y493H, R539T, I543T, C580Y), and PfATPase6 (S679S, M699V, S769M) associated with delayed clearance were determined by Taqman allelic discrimination and sequencing and list of primers used provided in Additional file 1: (Tables S1, 2 ). falciparum drug resistance genes; Pfcrt, Pfmdr1, PfATPase6 and Pfk13 that have been implicated in reduced ACT efficacy [2, 3, 9, 14] .	./cache/cord-312954-pnmycagi.txt	./txt/cord-312954-pnmycagi.txt