id sid tid token lemma pos cord-255936-hfa9w2dg 1 1 key key JJ cord-255936-hfa9w2dg 1 2 : : : cord-255936-hfa9w2dg 1 3 cord-255936-hfa9w2dg cord-255936-hfa9w2dg DT cord-255936-hfa9w2dg 1 4 authors author NNS cord-255936-hfa9w2dg 1 5 : : : cord-255936-hfa9w2dg 1 6 Jin Jin NNP cord-255936-hfa9w2dg 1 7 , , , cord-255936-hfa9w2dg 1 8 Junyeong Junyeong NNP cord-255936-hfa9w2dg 1 9 ; ; : cord-255936-hfa9w2dg 1 10 Park Park NNP cord-255936-hfa9w2dg 1 11 , , , cord-255936-hfa9w2dg 1 12 Changhoon Changhoon NNP cord-255936-hfa9w2dg 1 13 ; ; : cord-255936-hfa9w2dg 1 14 Cho Cho NNP cord-255936-hfa9w2dg 1 15 , , , cord-255936-hfa9w2dg 1 16 Sun Sun NNP cord-255936-hfa9w2dg 1 17 - - HYPH cord-255936-hfa9w2dg 1 18 Hee Hee NNP cord-255936-hfa9w2dg 1 19 ; ; : cord-255936-hfa9w2dg 1 20 Chung Chung NNP cord-255936-hfa9w2dg 1 21 , , , cord-255936-hfa9w2dg 1 22 Junho Junho NNP cord-255936-hfa9w2dg 2 1 title title NN cord-255936-hfa9w2dg 2 2 : : : cord-255936-hfa9w2dg 3 1 The the DT cord-255936-hfa9w2dg 3 2 level level NN cord-255936-hfa9w2dg 3 3 of of IN cord-255936-hfa9w2dg 3 4 decoy decoy NN cord-255936-hfa9w2dg 3 5 epitope epitope NN cord-255936-hfa9w2dg 3 6 in in IN cord-255936-hfa9w2dg 3 7 PCV2 pcv2 JJ cord-255936-hfa9w2dg 3 8 vaccine vaccine NN cord-255936-hfa9w2dg 3 9 affects affect VBZ cord-255936-hfa9w2dg 3 10 the the DT cord-255936-hfa9w2dg 3 11 neutralizing neutralize VBG cord-255936-hfa9w2dg 3 12 activity activity NN cord-255936-hfa9w2dg 3 13 of of IN cord-255936-hfa9w2dg 3 14 sera sera NNP cord-255936-hfa9w2dg 3 15 in in IN cord-255936-hfa9w2dg 3 16 the the DT cord-255936-hfa9w2dg 3 17 immunized immunize VBN cord-255936-hfa9w2dg 3 18 animals animal NNS cord-255936-hfa9w2dg 3 19 date date NN cord-255936-hfa9w2dg 3 20 : : : cord-255936-hfa9w2dg 3 21 2018 2018 CD cord-255936-hfa9w2dg 3 22 - - HYPH cord-255936-hfa9w2dg 3 23 02 02 CD cord-255936-hfa9w2dg 3 24 - - SYM cord-255936-hfa9w2dg 3 25 12 12 CD cord-255936-hfa9w2dg 3 26 journal journal NN cord-255936-hfa9w2dg 3 27 : : : cord-255936-hfa9w2dg 4 1 Biochemical biochemical JJ cord-255936-hfa9w2dg 4 2 and and CC cord-255936-hfa9w2dg 4 3 Biophysical Biophysical NNP cord-255936-hfa9w2dg 4 4 Research Research NNP cord-255936-hfa9w2dg 4 5 Communications Communications NNPS cord-255936-hfa9w2dg 4 6 DOI DOI NNP cord-255936-hfa9w2dg 4 7 : : : cord-255936-hfa9w2dg 4 8 10.1016 10.1016 CD cord-255936-hfa9w2dg 4 9 / / SYM cord-255936-hfa9w2dg 4 10 j.bbrc.2018.01.141 j.bbrc.2018.01.141 CD cord-255936-hfa9w2dg 4 11 sha sha NNP cord-255936-hfa9w2dg 4 12 : : : cord-255936-hfa9w2dg 4 13 f63cd990f80bf23a1c1ac68e58f8e6bcaa460cd8 f63cd990f80bf23a1c1ac68e58f8e6bcaa460cd8 NNP cord-255936-hfa9w2dg 4 14 doc_id doc_id NNP cord-255936-hfa9w2dg 4 15 : : : cord-255936-hfa9w2dg 4 16 255936 255936 CD cord-255936-hfa9w2dg 4 17 cord_uid cord_uid NNS cord-255936-hfa9w2dg 4 18 : : : cord-255936-hfa9w2dg 5 1 hfa9w2dg hfa9w2dg NNP cord-255936-hfa9w2dg 5 2 Abstract Abstract NNP cord-255936-hfa9w2dg 5 3 Viral viral JJ cord-255936-hfa9w2dg 5 4 pathogens pathogen NNS cord-255936-hfa9w2dg 5 5 have have VBP cord-255936-hfa9w2dg 5 6 evolved evolve VBN cord-255936-hfa9w2dg 5 7 a a DT cord-255936-hfa9w2dg 5 8 wide wide JJ cord-255936-hfa9w2dg 5 9 range range NN cord-255936-hfa9w2dg 5 10 of of IN cord-255936-hfa9w2dg 5 11 tactics tactic NNS cord-255936-hfa9w2dg 5 12 to to TO cord-255936-hfa9w2dg 5 13 evade evade VB cord-255936-hfa9w2dg 5 14 host host NN cord-255936-hfa9w2dg 5 15 immune immune JJ cord-255936-hfa9w2dg 5 16 responses response NNS cord-255936-hfa9w2dg 5 17 and and CC cord-255936-hfa9w2dg 5 18 thus thus RB cord-255936-hfa9w2dg 5 19 propagate propagate VB cord-255936-hfa9w2dg 5 20 effectively effectively RB cord-255936-hfa9w2dg 5 21 . . . cord-255936-hfa9w2dg 6 1 One one CD cord-255936-hfa9w2dg 6 2 efficient efficient JJ cord-255936-hfa9w2dg 6 3 tactic tactic NN cord-255936-hfa9w2dg 6 4 is be VBZ cord-255936-hfa9w2dg 6 5 to to TO cord-255936-hfa9w2dg 6 6 divert divert VB cord-255936-hfa9w2dg 6 7 host host NN cord-255936-hfa9w2dg 6 8 immune immune JJ cord-255936-hfa9w2dg 6 9 responses response NNS cord-255936-hfa9w2dg 6 10 toward toward IN cord-255936-hfa9w2dg 6 11 an an DT cord-255936-hfa9w2dg 6 12 immunodominant immunodominant JJ cord-255936-hfa9w2dg 6 13 decoy decoy NN cord-255936-hfa9w2dg 6 14 epitope epitope NN cord-255936-hfa9w2dg 6 15 and and CC cord-255936-hfa9w2dg 6 16 to to TO cord-255936-hfa9w2dg 6 17 induce induce VB cord-255936-hfa9w2dg 6 18 non non JJ cord-255936-hfa9w2dg 6 19 - - JJ cord-255936-hfa9w2dg 6 20 neutralizing neutralize VBG cord-255936-hfa9w2dg 6 21 antibodies antibody NNS cord-255936-hfa9w2dg 6 22 toward toward IN cord-255936-hfa9w2dg 6 23 this this DT cord-255936-hfa9w2dg 6 24 epitope epitope NN cord-255936-hfa9w2dg 6 25 . . . cord-255936-hfa9w2dg 7 1 Therefore therefore RB cord-255936-hfa9w2dg 7 2 , , , cord-255936-hfa9w2dg 7 3 it -PRON- PRP cord-255936-hfa9w2dg 7 4 is be VBZ cord-255936-hfa9w2dg 7 5 expected expect VBN cord-255936-hfa9w2dg 7 6 that that IN cord-255936-hfa9w2dg 7 7 the the DT cord-255936-hfa9w2dg 7 8 amount amount NN cord-255936-hfa9w2dg 7 9 of of IN cord-255936-hfa9w2dg 7 10 decoy decoy NN cord-255936-hfa9w2dg 7 11 epitope epitope NN cord-255936-hfa9w2dg 7 12 in in IN cord-255936-hfa9w2dg 7 13 a a DT cord-255936-hfa9w2dg 7 14 subunit subunit NN cord-255936-hfa9w2dg 7 15 vaccine vaccine NN cord-255936-hfa9w2dg 7 16 can can MD cord-255936-hfa9w2dg 7 17 affect affect VB cord-255936-hfa9w2dg 7 18 the the DT cord-255936-hfa9w2dg 7 19 level level NN cord-255936-hfa9w2dg 7 20 of of IN cord-255936-hfa9w2dg 7 21 neutralizing neutralize VBG cord-255936-hfa9w2dg 7 22 antibody antibody NN cord-255936-hfa9w2dg 7 23 in in IN cord-255936-hfa9w2dg 7 24 an an DT cord-255936-hfa9w2dg 7 25 immunized immunize VBN cord-255936-hfa9w2dg 7 26 animal animal NN cord-255936-hfa9w2dg 7 27 . . . cord-255936-hfa9w2dg 8 1 In in IN cord-255936-hfa9w2dg 8 2 this this DT cord-255936-hfa9w2dg 8 3 study study NN cord-255936-hfa9w2dg 8 4 , , , cord-255936-hfa9w2dg 8 5 we -PRON- PRP cord-255936-hfa9w2dg 8 6 tested test VBD cord-255936-hfa9w2dg 8 7 this this DT cord-255936-hfa9w2dg 8 8 hypothesis hypothesis NN cord-255936-hfa9w2dg 8 9 by by IN cord-255936-hfa9w2dg 8 10 generating generate VBG cord-255936-hfa9w2dg 8 11 an an DT cord-255936-hfa9w2dg 8 12 antibody antibody NN cord-255936-hfa9w2dg 8 13 specific specific JJ cord-255936-hfa9w2dg 8 14 to to IN cord-255936-hfa9w2dg 8 15 the the DT cord-255936-hfa9w2dg 8 16 decoy decoy NN cord-255936-hfa9w2dg 8 17 epitope epitope NN cord-255936-hfa9w2dg 8 18 on on IN cord-255936-hfa9w2dg 8 19 the the DT cord-255936-hfa9w2dg 8 20 capsid capsid JJ cord-255936-hfa9w2dg 8 21 protein protein NN cord-255936-hfa9w2dg 8 22 of of IN cord-255936-hfa9w2dg 8 23 porcine porcine JJ cord-255936-hfa9w2dg 8 24 circovirus circovirus NNP cord-255936-hfa9w2dg 8 25 type type NN cord-255936-hfa9w2dg 8 26 2 2 CD cord-255936-hfa9w2dg 8 27 ( ( -LRB- cord-255936-hfa9w2dg 8 28 PCV2 PCV2 NNP cord-255936-hfa9w2dg 8 29 ) ) -RRB- cord-255936-hfa9w2dg 8 30 . . . cord-255936-hfa9w2dg 9 1 Using use VBG cord-255936-hfa9w2dg 9 2 this this DT cord-255936-hfa9w2dg 9 3 antibody antibody NN cord-255936-hfa9w2dg 9 4 , , , cord-255936-hfa9w2dg 9 5 we -PRON- PRP cord-255936-hfa9w2dg 9 6 found find VBD cord-255936-hfa9w2dg 9 7 that that IN cord-255936-hfa9w2dg 9 8 two two CD cord-255936-hfa9w2dg 9 9 commercial commercial JJ cord-255936-hfa9w2dg 9 10 vaccines vaccine NNS cord-255936-hfa9w2dg 9 11 contained contain VBN cord-255936-hfa9w2dg 9 12 statistically statistically RB cord-255936-hfa9w2dg 9 13 different different JJ cord-255936-hfa9w2dg 9 14 amounts amount NNS cord-255936-hfa9w2dg 9 15 of of IN cord-255936-hfa9w2dg 9 16 the the DT cord-255936-hfa9w2dg 9 17 decoy decoy NN cord-255936-hfa9w2dg 9 18 epitope epitope NN cord-255936-hfa9w2dg 9 19 . . . cord-255936-hfa9w2dg 10 1 The the DT cord-255936-hfa9w2dg 10 2 vaccine vaccine NN cord-255936-hfa9w2dg 10 3 with with IN cord-255936-hfa9w2dg 10 4 lower low JJR cord-255936-hfa9w2dg 10 5 levels level NNS cord-255936-hfa9w2dg 10 6 of of IN cord-255936-hfa9w2dg 10 7 decoy decoy NN cord-255936-hfa9w2dg 10 8 epitope epitope NN cord-255936-hfa9w2dg 10 9 induced induce VBD cord-255936-hfa9w2dg 10 10 a a DT cord-255936-hfa9w2dg 10 11 significantly significantly RB cord-255936-hfa9w2dg 10 12 higher high JJR cord-255936-hfa9w2dg 10 13 level level NN cord-255936-hfa9w2dg 10 14 of of IN cord-255936-hfa9w2dg 10 15 neutralizing neutralize VBG cord-255936-hfa9w2dg 10 16 antibody antibody NN cord-255936-hfa9w2dg 10 17 after after IN cord-255936-hfa9w2dg 10 18 immunization immunization NN cord-255936-hfa9w2dg 10 19 . . . cord-255936-hfa9w2dg 11 1 This this DT cord-255936-hfa9w2dg 11 2 antibody antibody NN cord-255936-hfa9w2dg 11 3 can can MD cord-255936-hfa9w2dg 11 4 be be VB cord-255936-hfa9w2dg 11 5 used use VBN cord-255936-hfa9w2dg 11 6 as as IN cord-255936-hfa9w2dg 11 7 an an DT cord-255936-hfa9w2dg 11 8 analytical analytical JJ cord-255936-hfa9w2dg 11 9 tool tool NN cord-255936-hfa9w2dg 11 10 to to TO cord-255936-hfa9w2dg 11 11 monitor monitor VB cord-255936-hfa9w2dg 11 12 the the DT cord-255936-hfa9w2dg 11 13 quality quality NN cord-255936-hfa9w2dg 11 14 of of IN cord-255936-hfa9w2dg 11 15 a a DT cord-255936-hfa9w2dg 11 16 vaccine vaccine NN cord-255936-hfa9w2dg 11 17 from from IN cord-255936-hfa9w2dg 11 18 batch batch NN cord-255936-hfa9w2dg 11 19 to to TO cord-255936-hfa9w2dg 11 20 batch batch VB cord-255936-hfa9w2dg 11 21 . . . cord-255936-hfa9w2dg 12 1 Viral viral JJ cord-255936-hfa9w2dg 12 2 pathogens pathogen NNS cord-255936-hfa9w2dg 12 3 have have VBP cord-255936-hfa9w2dg 12 4 evolved evolve VBN cord-255936-hfa9w2dg 12 5 a a DT cord-255936-hfa9w2dg 12 6 wide wide JJ cord-255936-hfa9w2dg 12 7 range range NN cord-255936-hfa9w2dg 12 8 of of IN cord-255936-hfa9w2dg 12 9 tactics tactic NNS cord-255936-hfa9w2dg 12 10 to to TO cord-255936-hfa9w2dg 12 11 evade evade VB cord-255936-hfa9w2dg 12 12 host host NN cord-255936-hfa9w2dg 12 13 immune immune JJ cord-255936-hfa9w2dg 12 14 responses response NNS cord-255936-hfa9w2dg 12 15 and and CC cord-255936-hfa9w2dg 12 16 propagate propagate VBP cord-255936-hfa9w2dg 12 17 effectively effectively RB cord-255936-hfa9w2dg 12 18 [ [ -LRB- cord-255936-hfa9w2dg 12 19 1 1 CD cord-255936-hfa9w2dg 12 20 ] ] -RRB- cord-255936-hfa9w2dg 12 21 . . . cord-255936-hfa9w2dg 13 1 One one CD cord-255936-hfa9w2dg 13 2 such such JJ cord-255936-hfa9w2dg 13 3 tactic tactic NN cord-255936-hfa9w2dg 13 4 is be VBZ cord-255936-hfa9w2dg 13 5 to to TO cord-255936-hfa9w2dg 13 6 divert divert VB cord-255936-hfa9w2dg 13 7 the the DT cord-255936-hfa9w2dg 13 8 host host NN cord-255936-hfa9w2dg 13 9 immune immune JJ cord-255936-hfa9w2dg 13 10 response response NN cord-255936-hfa9w2dg 13 11 with with IN cord-255936-hfa9w2dg 13 12 a a DT cord-255936-hfa9w2dg 13 13 decoy decoy NN cord-255936-hfa9w2dg 13 14 . . . cord-255936-hfa9w2dg 14 1 Decoy decoy NN cord-255936-hfa9w2dg 14 2 epitopes epitope NNS cord-255936-hfa9w2dg 14 3 have have VBP cord-255936-hfa9w2dg 14 4 been be VBN cord-255936-hfa9w2dg 14 5 reported report VBN cord-255936-hfa9w2dg 14 6 in in IN cord-255936-hfa9w2dg 14 7 a a DT cord-255936-hfa9w2dg 14 8 wide wide JJ cord-255936-hfa9w2dg 14 9 variety variety NN cord-255936-hfa9w2dg 14 10 of of IN cord-255936-hfa9w2dg 14 11 viruses virus NNS cord-255936-hfa9w2dg 14 12 , , , cord-255936-hfa9w2dg 14 13 including include VBG cord-255936-hfa9w2dg 14 14 human human JJ cord-255936-hfa9w2dg 14 15 immunodeficiency immunodeficiency NN cord-255936-hfa9w2dg 14 16 virus virus NN cord-255936-hfa9w2dg 14 17 ( ( -LRB- cord-255936-hfa9w2dg 14 18 HIV HIV NNP cord-255936-hfa9w2dg 14 19 ) ) -RRB- cord-255936-hfa9w2dg 14 20 [ [ -LRB- cord-255936-hfa9w2dg 14 21 2 2 CD cord-255936-hfa9w2dg 14 22 ] ] -RRB- cord-255936-hfa9w2dg 14 23 , , , cord-255936-hfa9w2dg 14 24 feline feline JJ cord-255936-hfa9w2dg 14 25 immunodeficiency immunodeficiency NN cord-255936-hfa9w2dg 14 26 virus virus NN cord-255936-hfa9w2dg 14 27 ( ( -LRB- cord-255936-hfa9w2dg 14 28 FIV FIV NNP cord-255936-hfa9w2dg 14 29 ) ) -RRB- cord-255936-hfa9w2dg 14 30 [ [ -LRB- cord-255936-hfa9w2dg 14 31 3 3 CD cord-255936-hfa9w2dg 14 32 ] ] -RRB- cord-255936-hfa9w2dg 14 33 , , , cord-255936-hfa9w2dg 14 34 hepatitis hepatitis NN cord-255936-hfa9w2dg 14 35 C C NNP cord-255936-hfa9w2dg 14 36 [ [ -LRB- cord-255936-hfa9w2dg 14 37 4 4 CD cord-255936-hfa9w2dg 14 38 ] ] -RRB- cord-255936-hfa9w2dg 14 39 , , , cord-255936-hfa9w2dg 14 40 foot foot NN cord-255936-hfa9w2dg 14 41 and and CC cord-255936-hfa9w2dg 14 42 mouth mouth NN cord-255936-hfa9w2dg 14 43 disease disease NN cord-255936-hfa9w2dg 14 44 [ [ -LRB- cord-255936-hfa9w2dg 14 45 5 5 CD cord-255936-hfa9w2dg 14 46 ] ] -RRB- cord-255936-hfa9w2dg 14 47 , , , cord-255936-hfa9w2dg 14 48 middleeast middleeast NNP cord-255936-hfa9w2dg 14 49 respiratory respiratory JJ cord-255936-hfa9w2dg 14 50 syndrome syndrome NN cord-255936-hfa9w2dg 14 51 coronavirus coronavirus NN cord-255936-hfa9w2dg 14 52 [ [ -LRB- cord-255936-hfa9w2dg 14 53 6 6 CD cord-255936-hfa9w2dg 14 54 ] ] -RRB- cord-255936-hfa9w2dg 14 55 , , , cord-255936-hfa9w2dg 14 56 severe severe JJ cord-255936-hfa9w2dg 14 57 fever fever NN cord-255936-hfa9w2dg 14 58 with with IN cord-255936-hfa9w2dg 14 59 thrombocytopenia thrombocytopenia NNP cord-255936-hfa9w2dg 14 60 syndrome syndrome NN cord-255936-hfa9w2dg 14 61 virus virus NN cord-255936-hfa9w2dg 14 62 [ [ -LRB- cord-255936-hfa9w2dg 14 63 7 7 CD cord-255936-hfa9w2dg 14 64 ] ] -RRB- cord-255936-hfa9w2dg 14 65 , , , cord-255936-hfa9w2dg 14 66 porcine porcine JJ cord-255936-hfa9w2dg 14 67 reproductive reproductive JJ cord-255936-hfa9w2dg 14 68 and and CC cord-255936-hfa9w2dg 14 69 respiratory respiratory JJ cord-255936-hfa9w2dg 14 70 syndrome syndrome NN cord-255936-hfa9w2dg 14 71 virus virus NN cord-255936-hfa9w2dg 14 72 ( ( -LRB- cord-255936-hfa9w2dg 14 73 PRRSV PRRSV NNP cord-255936-hfa9w2dg 14 74 ) ) -RRB- cord-255936-hfa9w2dg 15 1 [ [ -LRB- cord-255936-hfa9w2dg 15 2 8 8 CD cord-255936-hfa9w2dg 15 3 ] ] -RRB- cord-255936-hfa9w2dg 15 4 , , , cord-255936-hfa9w2dg 15 5 murine murine JJ cord-255936-hfa9w2dg 15 6 gammaherpesvirus-68 gammaherpesvirus-68 NN cord-255936-hfa9w2dg 15 7 ( ( -LRB- cord-255936-hfa9w2dg 15 8 MHV-68 MHV-68 NNP cord-255936-hfa9w2dg 15 9 ) ) -RRB- cord-255936-hfa9w2dg 16 1 [ [ -LRB- cord-255936-hfa9w2dg 16 2 9 9 CD cord-255936-hfa9w2dg 16 3 ] ] -RRB- cord-255936-hfa9w2dg 16 4 , , , cord-255936-hfa9w2dg 16 5 and and CC cord-255936-hfa9w2dg 16 6 porcine porcine JJ cord-255936-hfa9w2dg 16 7 circovirus circovirus JJ cord-255936-hfa9w2dg 16 8 type type NN cord-255936-hfa9w2dg 16 9 2 2 CD cord-255936-hfa9w2dg 17 1 ( ( -LRB- cord-255936-hfa9w2dg 17 2 PCV2 pcv2 JJ cord-255936-hfa9w2dg 17 3 ) ) -RRB- cord-255936-hfa9w2dg 17 4 [ [ -LRB- cord-255936-hfa9w2dg 17 5 10 10 CD cord-255936-hfa9w2dg 17 6 ] ] -RRB- cord-255936-hfa9w2dg 17 7 . . . cord-255936-hfa9w2dg 18 1 Because because IN cord-255936-hfa9w2dg 18 2 a a DT cord-255936-hfa9w2dg 18 3 recombinant recombinant JJ cord-255936-hfa9w2dg 18 4 mutant mutant JJ cord-255936-hfa9w2dg 18 5 capsid capsid NN cord-255936-hfa9w2dg 18 6 protein protein NN cord-255936-hfa9w2dg 18 7 ( ( -LRB- cord-255936-hfa9w2dg 18 8 CP CP NNP cord-255936-hfa9w2dg 18 9 ) ) -RRB- cord-255936-hfa9w2dg 18 10 with with IN cord-255936-hfa9w2dg 18 11 a a DT cord-255936-hfa9w2dg 18 12 deleted delete VBN cord-255936-hfa9w2dg 18 13 decoy decoy NN cord-255936-hfa9w2dg 18 14 epitope epitope NN cord-255936-hfa9w2dg 18 15 induced induce VBD cord-255936-hfa9w2dg 18 16 a a DT cord-255936-hfa9w2dg 18 17 higher high JJR cord-255936-hfa9w2dg 18 18 level level NN cord-255936-hfa9w2dg 18 19 of of IN cord-255936-hfa9w2dg 18 20 virusneutralizing virusneutralize VBG cord-255936-hfa9w2dg 18 21 antibody antibody NN cord-255936-hfa9w2dg 18 22 than than IN cord-255936-hfa9w2dg 18 23 wild wild JJ cord-255936-hfa9w2dg 18 24 type type NN cord-255936-hfa9w2dg 18 25 proteins protein NNS cord-255936-hfa9w2dg 18 26 [ [ -LRB- cord-255936-hfa9w2dg 18 27 11 11 CD cord-255936-hfa9w2dg 18 28 ] ] -RRB- cord-255936-hfa9w2dg 18 29 , , , cord-255936-hfa9w2dg 18 30 it -PRON- PRP cord-255936-hfa9w2dg 18 31 was be VBD cord-255936-hfa9w2dg 18 32 expected expect VBN cord-255936-hfa9w2dg 18 33 that that IN cord-255936-hfa9w2dg 18 34 higher high JJR cord-255936-hfa9w2dg 18 35 levels level NNS cord-255936-hfa9w2dg 18 36 of of IN cord-255936-hfa9w2dg 18 37 decoy decoy NN cord-255936-hfa9w2dg 18 38 epitope epitope NN cord-255936-hfa9w2dg 18 39 in in IN cord-255936-hfa9w2dg 18 40 a a DT cord-255936-hfa9w2dg 18 41 prepared prepared JJ cord-255936-hfa9w2dg 18 42 batch batch NN cord-255936-hfa9w2dg 18 43 of of IN cord-255936-hfa9w2dg 18 44 subunit subunit JJ cord-255936-hfa9w2dg 18 45 vaccine vaccine NN cord-255936-hfa9w2dg 18 46 would would MD cord-255936-hfa9w2dg 18 47 decrease decrease VB cord-255936-hfa9w2dg 18 48 its -PRON- PRP$ cord-255936-hfa9w2dg 18 49 efficacy efficacy NN cord-255936-hfa9w2dg 18 50 . . . cord-255936-hfa9w2dg 19 1 However however RB cord-255936-hfa9w2dg 19 2 , , , cord-255936-hfa9w2dg 19 3 it -PRON- PRP cord-255936-hfa9w2dg 19 4 was be VBD cord-255936-hfa9w2dg 19 5 not not RB cord-255936-hfa9w2dg 19 6 possible possible JJ cord-255936-hfa9w2dg 19 7 to to TO cord-255936-hfa9w2dg 19 8 test test VB cord-255936-hfa9w2dg 19 9 this this DT cord-255936-hfa9w2dg 19 10 hypothesis hypothesis NN cord-255936-hfa9w2dg 19 11 by by IN cord-255936-hfa9w2dg 19 12 measuring measure VBG cord-255936-hfa9w2dg 19 13 levels level NNS cord-255936-hfa9w2dg 19 14 of of IN cord-255936-hfa9w2dg 19 15 decoy decoy NN cord-255936-hfa9w2dg 19 16 epitope epitope NN cord-255936-hfa9w2dg 19 17 in in IN cord-255936-hfa9w2dg 19 18 a a DT cord-255936-hfa9w2dg 19 19 vaccine vaccine NN cord-255936-hfa9w2dg 19 20 because because IN cord-255936-hfa9w2dg 19 21 an an DT cord-255936-hfa9w2dg 19 22 antibody antibody NN cord-255936-hfa9w2dg 19 23 specific specific JJ cord-255936-hfa9w2dg 19 24 to to IN cord-255936-hfa9w2dg 19 25 the the DT cord-255936-hfa9w2dg 19 26 decoy decoy NN cord-255936-hfa9w2dg 19 27 epitope epitope NN cord-255936-hfa9w2dg 19 28 was be VBD cord-255936-hfa9w2dg 19 29 not not RB cord-255936-hfa9w2dg 19 30 readily readily RB cord-255936-hfa9w2dg 19 31 available available JJ cord-255936-hfa9w2dg 19 32 . . . cord-255936-hfa9w2dg 20 1 PCV2 PCV2 NNP cord-255936-hfa9w2dg 20 2 has have VBZ cord-255936-hfa9w2dg 20 3 a a DT cord-255936-hfa9w2dg 20 4 spherical spherical JJ cord-255936-hfa9w2dg 20 5 structure structure NN cord-255936-hfa9w2dg 20 6 with with IN cord-255936-hfa9w2dg 20 7 icosahedral icosahedral JJ cord-255936-hfa9w2dg 20 8 symmetry symmetry NN cord-255936-hfa9w2dg 20 9 [ [ -LRB- cord-255936-hfa9w2dg 20 10 12 12 CD cord-255936-hfa9w2dg 20 11 ] ] -RRB- cord-255936-hfa9w2dg 20 12 . . . cord-255936-hfa9w2dg 21 1 The the DT cord-255936-hfa9w2dg 21 2 only only JJ cord-255936-hfa9w2dg 21 3 structural structural JJ cord-255936-hfa9w2dg 21 4 protein protein NN cord-255936-hfa9w2dg 21 5 of of IN cord-255936-hfa9w2dg 21 6 this this DT cord-255936-hfa9w2dg 21 7 virus virus NN cord-255936-hfa9w2dg 21 8 is be VBZ cord-255936-hfa9w2dg 21 9 a a DT cord-255936-hfa9w2dg 21 10 28-kD 28-kd CD cord-255936-hfa9w2dg 21 11 CP cp NN cord-255936-hfa9w2dg 21 12 , , , cord-255936-hfa9w2dg 21 13 which which WDT cord-255936-hfa9w2dg 21 14 includes include VBZ cord-255936-hfa9w2dg 21 15 major major JJ cord-255936-hfa9w2dg 21 16 antigenic antigenic JJ cord-255936-hfa9w2dg 21 17 determinants determinant NNS cord-255936-hfa9w2dg 21 18 that that WDT cord-255936-hfa9w2dg 21 19 can can MD cord-255936-hfa9w2dg 21 20 be be VB cord-255936-hfa9w2dg 21 21 used use VBN cord-255936-hfa9w2dg 21 22 in in IN cord-255936-hfa9w2dg 21 23 subunit subunit NN cord-255936-hfa9w2dg 21 24 vaccines vaccine NNS cord-255936-hfa9w2dg 21 25 [ [ -LRB- cord-255936-hfa9w2dg 21 26 13 13 CD cord-255936-hfa9w2dg 21 27 ] ] -RRB- cord-255936-hfa9w2dg 21 28 . . . cord-255936-hfa9w2dg 22 1 A a DT cord-255936-hfa9w2dg 22 2 previous previous JJ cord-255936-hfa9w2dg 22 3 study study NN cord-255936-hfa9w2dg 22 4 showed show VBD cord-255936-hfa9w2dg 22 5 that that IN cord-255936-hfa9w2dg 22 6 an an DT cord-255936-hfa9w2dg 22 7 epitope epitope NN cord-255936-hfa9w2dg 22 8 composed compose VBN cord-255936-hfa9w2dg 22 9 of of IN cord-255936-hfa9w2dg 22 10 amino amino JJ cord-255936-hfa9w2dg 22 11 acid acid NN cord-255936-hfa9w2dg 22 12 residues residue NNS cord-255936-hfa9w2dg 22 13 169e180 169e180 CD cord-255936-hfa9w2dg 23 1 ( ( -LRB- cord-255936-hfa9w2dg 23 2 CP CP NNP cord-255936-hfa9w2dg 23 3 169e180 169e180 NNP cord-255936-hfa9w2dg 23 4 ) ) -RRB- cord-255936-hfa9w2dg 23 5 is be VBZ cord-255936-hfa9w2dg 23 6 the the DT cord-255936-hfa9w2dg 23 7 most most RBS cord-255936-hfa9w2dg 23 8 immunodominant immunodominant JJ cord-255936-hfa9w2dg 23 9 among among IN cord-255936-hfa9w2dg 23 10 these these DT cord-255936-hfa9w2dg 23 11 antigenic antigenic JJ cord-255936-hfa9w2dg 23 12 determinants determinant NNS cord-255936-hfa9w2dg 23 13 [ [ -LRB- cord-255936-hfa9w2dg 23 14 11 11 CD cord-255936-hfa9w2dg 23 15 ] ] -RRB- cord-255936-hfa9w2dg 23 16 . . . cord-255936-hfa9w2dg 24 1 Because because IN cord-255936-hfa9w2dg 24 2 this this DT cord-255936-hfa9w2dg 24 3 epitope epitope NN cord-255936-hfa9w2dg 24 4 is be VBZ cord-255936-hfa9w2dg 24 5 buried bury VBN cord-255936-hfa9w2dg 24 6 inside inside IN cord-255936-hfa9w2dg 24 7 the the DT cord-255936-hfa9w2dg 24 8 virus virus NN cord-255936-hfa9w2dg 24 9 - - HYPH cord-255936-hfa9w2dg 24 10 like like JJ cord-255936-hfa9w2dg 24 11 particle particle NN cord-255936-hfa9w2dg 24 12 ( ( -LRB- cord-255936-hfa9w2dg 24 13 VLP VLP NNP cord-255936-hfa9w2dg 24 14 ) ) -RRB- cord-255936-hfa9w2dg 24 15 structure structure NN cord-255936-hfa9w2dg 24 16 ( ( -LRB- cord-255936-hfa9w2dg 24 17 Fig Fig NNP cord-255936-hfa9w2dg 24 18 . . . cord-255936-hfa9w2dg 24 19 1A 1a NN cord-255936-hfa9w2dg 24 20 ) ) -RRB- cord-255936-hfa9w2dg 24 21 , , , cord-255936-hfa9w2dg 24 22 antibodies antibody NNS cord-255936-hfa9w2dg 24 23 to to IN cord-255936-hfa9w2dg 24 24 this this DT cord-255936-hfa9w2dg 24 25 epitope epitope NN cord-255936-hfa9w2dg 24 26 can can MD cord-255936-hfa9w2dg 24 27 not not RB cord-255936-hfa9w2dg 24 28 react react VB cord-255936-hfa9w2dg 24 29 with with IN cord-255936-hfa9w2dg 24 30 the the DT cord-255936-hfa9w2dg 24 31 intact intact JJ cord-255936-hfa9w2dg 24 32 virus virus NN cord-255936-hfa9w2dg 24 33 , , , cord-255936-hfa9w2dg 24 34 and and CC cord-255936-hfa9w2dg 24 35 therefore therefore RB cord-255936-hfa9w2dg 24 36 are be VBP cord-255936-hfa9w2dg 24 37 non non JJ cord-255936-hfa9w2dg 24 38 - - JJ cord-255936-hfa9w2dg 24 39 neutralizing neutralize VBG cord-255936-hfa9w2dg 24 40 [ [ -LRB- cord-255936-hfa9w2dg 24 41 14 14 CD cord-255936-hfa9w2dg 24 42 ] ] -RRB- cord-255936-hfa9w2dg 24 43 . . . cord-255936-hfa9w2dg 25 1 As as IN cord-255936-hfa9w2dg 25 2 expected expect VBN cord-255936-hfa9w2dg 25 3 , , , cord-255936-hfa9w2dg 25 4 PCV PCV NNP cord-255936-hfa9w2dg 25 5 infection infection NN cord-255936-hfa9w2dg 25 6 elicited elicit VBD cord-255936-hfa9w2dg 25 7 a a DT cord-255936-hfa9w2dg 25 8 humoral humoral JJ cord-255936-hfa9w2dg 25 9 response response NN cord-255936-hfa9w2dg 25 10 to to IN cord-255936-hfa9w2dg 25 11 the the DT cord-255936-hfa9w2dg 25 12 CP CP NNP cord-255936-hfa9w2dg 25 13 169e180 169e180 CD cord-255936-hfa9w2dg 25 14 epitope epitope NN cord-255936-hfa9w2dg 25 15 and and CC cord-255936-hfa9w2dg 25 16 drove drive VBD cord-255936-hfa9w2dg 25 17 the the DT cord-255936-hfa9w2dg 25 18 production production NN cord-255936-hfa9w2dg 25 19 of of IN cord-255936-hfa9w2dg 25 20 non non JJ cord-255936-hfa9w2dg 25 21 - - JJ cord-255936-hfa9w2dg 25 22 neutralizing neutralize VBG cord-255936-hfa9w2dg 25 23 antibodies antibody NNS cord-255936-hfa9w2dg 25 24 [ [ -LRB- cord-255936-hfa9w2dg 25 25 15 15 CD cord-255936-hfa9w2dg 25 26 ] ] -RRB- cord-255936-hfa9w2dg 25 27 . . . cord-255936-hfa9w2dg 26 1 Therefore therefore RB cord-255936-hfa9w2dg 26 2 , , , cord-255936-hfa9w2dg 26 3 previous previous JJ cord-255936-hfa9w2dg 26 4 infection infection NN cord-255936-hfa9w2dg 26 5 with with IN cord-255936-hfa9w2dg 26 6 PCV PCV NNP cord-255936-hfa9w2dg 26 7 decreased decrease VBD cord-255936-hfa9w2dg 26 8 the the DT cord-255936-hfa9w2dg 26 9 vaccines vaccine NNS cord-255936-hfa9w2dg 26 10 prevention prevention NN cord-255936-hfa9w2dg 26 11 of of IN cord-255936-hfa9w2dg 26 12 the the DT cord-255936-hfa9w2dg 26 13 future future JJ cord-255936-hfa9w2dg 26 14 infections infection NNS cord-255936-hfa9w2dg 26 15 . . . cord-255936-hfa9w2dg 27 1 Since since IN cord-255936-hfa9w2dg 27 2 the the DT cord-255936-hfa9w2dg 27 3 first first JJ cord-255936-hfa9w2dg 27 4 vaccine vaccine NN cord-255936-hfa9w2dg 27 5 against against IN cord-255936-hfa9w2dg 27 6 PCV2 PCV2 NNP cord-255936-hfa9w2dg 27 7 was be VBD cord-255936-hfa9w2dg 27 8 introduced introduce VBN cord-255936-hfa9w2dg 27 9 to to IN cord-255936-hfa9w2dg 27 10 the the DT cord-255936-hfa9w2dg 27 11 global global JJ cord-255936-hfa9w2dg 27 12 market market NN cord-255936-hfa9w2dg 27 13 in in IN cord-255936-hfa9w2dg 27 14 2006 2006 CD cord-255936-hfa9w2dg 27 15 , , , cord-255936-hfa9w2dg 27 16 nine nine CD cord-255936-hfa9w2dg 27 17 PCV2 PCV2 NNP cord-255936-hfa9w2dg 27 18 subunit subunit NN cord-255936-hfa9w2dg 27 19 vaccines vaccine NNS cord-255936-hfa9w2dg 27 20 have have VBP cord-255936-hfa9w2dg 27 21 become become VBN cord-255936-hfa9w2dg 27 22 available available JJ cord-255936-hfa9w2dg 27 23 [ [ -LRB- cord-255936-hfa9w2dg 27 24 16 16 CD cord-255936-hfa9w2dg 27 25 ] ] -RRB- cord-255936-hfa9w2dg 27 26 . . . cord-255936-hfa9w2dg 28 1 However however RB cord-255936-hfa9w2dg 28 2 , , , cord-255936-hfa9w2dg 28 3 they -PRON- PRP cord-255936-hfa9w2dg 28 4 were be VBD cord-255936-hfa9w2dg 28 5 expected expect VBN cord-255936-hfa9w2dg 28 6 to to TO cord-255936-hfa9w2dg 28 7 contain contain VB cord-255936-hfa9w2dg 28 8 differing differ VBG cord-255936-hfa9w2dg 28 9 amounts amount NNS cord-255936-hfa9w2dg 28 10 of of IN cord-255936-hfa9w2dg 28 11 incomplete incomplete JJ cord-255936-hfa9w2dg 28 12 VLPs vlp NNS cord-255936-hfa9w2dg 28 13 , , , cord-255936-hfa9w2dg 28 14 which which WDT cord-255936-hfa9w2dg 28 15 are be VBP cord-255936-hfa9w2dg 28 16 non non JJ cord-255936-hfa9w2dg 28 17 - - JJ cord-255936-hfa9w2dg 28 18 uniformly uniformly RB cord-255936-hfa9w2dg 28 19 aggregated aggregate VBN cord-255936-hfa9w2dg 28 20 CPs cp NNS cord-255936-hfa9w2dg 28 21 with with IN cord-255936-hfa9w2dg 28 22 an an DT cord-255936-hfa9w2dg 28 23 exposed expose VBN cord-255936-hfa9w2dg 28 24 CP CP NNP cord-255936-hfa9w2dg 28 25 169e180 169e180 CD cord-255936-hfa9w2dg 28 26 epitope epitope NN cord-255936-hfa9w2dg 28 27 [ [ -LRB- cord-255936-hfa9w2dg 28 28 17 17 CD cord-255936-hfa9w2dg 28 29 ] ] -RRB- cord-255936-hfa9w2dg 28 30 . . . cord-255936-hfa9w2dg 29 1 Incomplete incomplete JJ cord-255936-hfa9w2dg 29 2 VLPs vlp NNS cord-255936-hfa9w2dg 29 3 can can MD cord-255936-hfa9w2dg 29 4 induce induce VB cord-255936-hfa9w2dg 29 5 production production NN cord-255936-hfa9w2dg 29 6 of of IN cord-255936-hfa9w2dg 29 7 non non JJ cord-255936-hfa9w2dg 29 8 - - JJ cord-255936-hfa9w2dg 29 9 neutralizing neutralize VBG cord-255936-hfa9w2dg 29 10 antibodies antibody NNS cord-255936-hfa9w2dg 29 11 and and CC cord-255936-hfa9w2dg 29 12 reduce reduce VB cord-255936-hfa9w2dg 29 13 vaccine vaccine NN cord-255936-hfa9w2dg 29 14 efficacy efficacy NN cord-255936-hfa9w2dg 29 15 [ [ -LRB- cord-255936-hfa9w2dg 29 16 14 14 CD cord-255936-hfa9w2dg 29 17 , , , cord-255936-hfa9w2dg 29 18 18 18 CD cord-255936-hfa9w2dg 29 19 ] ] -RRB- cord-255936-hfa9w2dg 29 20 . . . cord-255936-hfa9w2dg 30 1 As as IN cord-255936-hfa9w2dg 30 2 there there EX cord-255936-hfa9w2dg 30 3 was be VBD cord-255936-hfa9w2dg 30 4 no no DT cord-255936-hfa9w2dg 30 5 analytical analytical JJ cord-255936-hfa9w2dg 30 6 tool tool NN cord-255936-hfa9w2dg 30 7 to to TO cord-255936-hfa9w2dg 30 8 measure measure VB cord-255936-hfa9w2dg 30 9 the the DT cord-255936-hfa9w2dg 30 10 amount amount NN cord-255936-hfa9w2dg 30 11 of of IN cord-255936-hfa9w2dg 30 12 CP CP NNP cord-255936-hfa9w2dg 30 13 169e180 169e180 CD cord-255936-hfa9w2dg 30 14 epitope epitope NN cord-255936-hfa9w2dg 30 15 in in IN cord-255936-hfa9w2dg 30 16 a a DT cord-255936-hfa9w2dg 30 17 prepared prepared JJ cord-255936-hfa9w2dg 30 18 batch batch NN cord-255936-hfa9w2dg 30 19 of of IN cord-255936-hfa9w2dg 30 20 vaccine vaccine NN cord-255936-hfa9w2dg 30 21 , , , cord-255936-hfa9w2dg 30 22 it -PRON- PRP cord-255936-hfa9w2dg 30 23 was be VBD cord-255936-hfa9w2dg 30 24 not not RB cord-255936-hfa9w2dg 30 25 possible possible JJ cord-255936-hfa9w2dg 30 26 to to TO cord-255936-hfa9w2dg 30 27 correlate correlate VB cord-255936-hfa9w2dg 30 28 epitope epitope NN cord-255936-hfa9w2dg 30 29 levels level NNS cord-255936-hfa9w2dg 30 30 with with IN cord-255936-hfa9w2dg 30 31 neutralizing neutralize VBG cord-255936-hfa9w2dg 30 32 activity activity NN cord-255936-hfa9w2dg 30 33 after after IN cord-255936-hfa9w2dg 30 34 vaccination vaccination NN cord-255936-hfa9w2dg 30 35 . . . cord-255936-hfa9w2dg 31 1 In in IN cord-255936-hfa9w2dg 31 2 this this DT cord-255936-hfa9w2dg 31 3 study study NN cord-255936-hfa9w2dg 31 4 , , , cord-255936-hfa9w2dg 31 5 we -PRON- PRP cord-255936-hfa9w2dg 31 6 developed develop VBD cord-255936-hfa9w2dg 31 7 an an DT cord-255936-hfa9w2dg 31 8 antibody antibody NN cord-255936-hfa9w2dg 31 9 specific specific JJ cord-255936-hfa9w2dg 31 10 to to IN cord-255936-hfa9w2dg 31 11 the the DT cord-255936-hfa9w2dg 31 12 CP CP NNP cord-255936-hfa9w2dg 31 13 169e180 169e180 CD cord-255936-hfa9w2dg 31 14 epitope epitope NN cord-255936-hfa9w2dg 31 15 , , , cord-255936-hfa9w2dg 31 16 determined determine VBD cord-255936-hfa9w2dg 31 17 the the DT cord-255936-hfa9w2dg 31 18 relative relative JJ cord-255936-hfa9w2dg 31 19 amounts amount NNS cord-255936-hfa9w2dg 31 20 of of IN cord-255936-hfa9w2dg 31 21 CP CP NNP cord-255936-hfa9w2dg 31 22 169e180 169e180 CD cord-255936-hfa9w2dg 31 23 epitope epitope NN cord-255936-hfa9w2dg 31 24 in in IN cord-255936-hfa9w2dg 31 25 two two CD cord-255936-hfa9w2dg 31 26 commercially commercially RB cord-255936-hfa9w2dg 31 27 available available JJ cord-255936-hfa9w2dg 31 28 vaccines vaccine NNS cord-255936-hfa9w2dg 31 29 , , , cord-255936-hfa9w2dg 31 30 and and CC cord-255936-hfa9w2dg 31 31 analyzed analyze VBD cord-255936-hfa9w2dg 31 32 the the DT cord-255936-hfa9w2dg 31 33 influence influence NN cord-255936-hfa9w2dg 31 34 of of IN cord-255936-hfa9w2dg 31 35 the the DT cord-255936-hfa9w2dg 31 36 epitope epitope NN cord-255936-hfa9w2dg 31 37 level level NN cord-255936-hfa9w2dg 31 38 on on IN cord-255936-hfa9w2dg 31 39 the the DT cord-255936-hfa9w2dg 31 40 induction induction NN cord-255936-hfa9w2dg 31 41 of of IN cord-255936-hfa9w2dg 31 42 neutralizing neutralize VBG cord-255936-hfa9w2dg 31 43 antibody antibody NN cord-255936-hfa9w2dg 31 44 . . . cord-255936-hfa9w2dg 32 1 2.1 2.1 CD cord-255936-hfa9w2dg 32 2 . . . cord-255936-hfa9w2dg 33 1 Preparation preparation NN cord-255936-hfa9w2dg 33 2 of of IN cord-255936-hfa9w2dg 33 3 VLP VLP NNP cord-255936-hfa9w2dg 33 4 , , , cord-255936-hfa9w2dg 33 5 PCV2 PCV2 NNP cord-255936-hfa9w2dg 33 6 recombinant recombinant JJ cord-255936-hfa9w2dg 33 7 proteins protein NNS cord-255936-hfa9w2dg 33 8 , , , cord-255936-hfa9w2dg 33 9 and and CC cord-255936-hfa9w2dg 33 10 peptide peptide NN cord-255936-hfa9w2dg 33 11 conjugates conjugate NNS cord-255936-hfa9w2dg 34 1 PCV2 pcv2 JJ cord-255936-hfa9w2dg 34 2 CP cp NN cord-255936-hfa9w2dg 34 3 ( ( -LRB- cord-255936-hfa9w2dg 34 4 amino amino NN cord-255936-hfa9w2dg 34 5 acids acid NNS cord-255936-hfa9w2dg 34 6 [ [ -LRB- cord-255936-hfa9w2dg 34 7 aa aa NNP cord-255936-hfa9w2dg 34 8 ] ] -RRB- cord-255936-hfa9w2dg 34 9 1e233 1e233 CD cord-255936-hfa9w2dg 34 10 ) ) -RRB- cord-255936-hfa9w2dg 34 11 was be VBD cord-255936-hfa9w2dg 34 12 expressed express VBN cord-255936-hfa9w2dg 34 13 in in IN cord-255936-hfa9w2dg 34 14 baculovirusinfected baculovirusinfected JJ cord-255936-hfa9w2dg 34 15 Sf9 sf9 JJ cord-255936-hfa9w2dg 34 16 insect insect NN cord-255936-hfa9w2dg 34 17 cells cell NNS cord-255936-hfa9w2dg 34 18 , , , cord-255936-hfa9w2dg 34 19 as as IN cord-255936-hfa9w2dg 34 20 previously previously RB cord-255936-hfa9w2dg 34 21 described describe VBN cord-255936-hfa9w2dg 34 22 [ [ -LRB- cord-255936-hfa9w2dg 34 23 19 19 CD cord-255936-hfa9w2dg 34 24 ] ] -RRB- cord-255936-hfa9w2dg 34 25 . . . cord-255936-hfa9w2dg 35 1 The the DT cord-255936-hfa9w2dg 35 2 PCV2 PCV2 NNP cord-255936-hfa9w2dg 35 3 ORF2 ORF2 NNP cord-255936-hfa9w2dg 35 4 gene gene NN cord-255936-hfa9w2dg 35 5 ( ( -LRB- cord-255936-hfa9w2dg 35 6 GenBank GenBank NNP cord-255936-hfa9w2dg 35 7 no no NN cord-255936-hfa9w2dg 35 8 . . . cord-255936-hfa9w2dg 36 1 EU747125 EU747125 NNP cord-255936-hfa9w2dg 36 2 ) ) -RRB- cord-255936-hfa9w2dg 36 3 was be VBD cord-255936-hfa9w2dg 36 4 cloned clone VBN cord-255936-hfa9w2dg 36 5 into into IN cord-255936-hfa9w2dg 36 6 the the DT cord-255936-hfa9w2dg 36 7 pFastBac pfastbac JJ cord-255936-hfa9w2dg 36 8 expression expression NN cord-255936-hfa9w2dg 36 9 vector vector NN cord-255936-hfa9w2dg 36 10 ( ( -LRB- cord-255936-hfa9w2dg 36 11 Invitrogen Invitrogen NNP cord-255936-hfa9w2dg 36 12 , , , cord-255936-hfa9w2dg 36 13 Carlsbad Carlsbad NNP cord-255936-hfa9w2dg 36 14 , , , cord-255936-hfa9w2dg 36 15 CA CA NNP cord-255936-hfa9w2dg 36 16 , , , cord-255936-hfa9w2dg 36 17 USA USA NNP cord-255936-hfa9w2dg 36 18 ) ) -RRB- cord-255936-hfa9w2dg 36 19 and and CC cord-255936-hfa9w2dg 36 20 transfected transfecte VBN cord-255936-hfa9w2dg 36 21 into into IN cord-255936-hfa9w2dg 36 22 Sf9 sf9 NN cord-255936-hfa9w2dg 36 23 cells cell NNS cord-255936-hfa9w2dg 36 24 . . . cord-255936-hfa9w2dg 37 1 The the DT cord-255936-hfa9w2dg 37 2 culture culture NN cord-255936-hfa9w2dg 37 3 supernatant supernatant NN cord-255936-hfa9w2dg 37 4 containing contain VBG cord-255936-hfa9w2dg 37 5 the the DT cord-255936-hfa9w2dg 37 6 recombinant recombinant JJ cord-255936-hfa9w2dg 37 7 baculovirus baculovirus NN cord-255936-hfa9w2dg 37 8 was be VBD cord-255936-hfa9w2dg 37 9 harvested harvest VBN cord-255936-hfa9w2dg 37 10 and and CC cord-255936-hfa9w2dg 37 11 used use VBN cord-255936-hfa9w2dg 37 12 to to TO cord-255936-hfa9w2dg 37 13 infect infect VB cord-255936-hfa9w2dg 37 14 a a DT cord-255936-hfa9w2dg 37 15 separate separate JJ cord-255936-hfa9w2dg 37 16 batch batch NN cord-255936-hfa9w2dg 37 17 of of IN cord-255936-hfa9w2dg 37 18 Sf9 sf9 NN cord-255936-hfa9w2dg 37 19 cells cell NNS cord-255936-hfa9w2dg 37 20 . . . cord-255936-hfa9w2dg 38 1 After after IN cord-255936-hfa9w2dg 38 2 3 3 CD cord-255936-hfa9w2dg 38 3 days day NNS cord-255936-hfa9w2dg 38 4 , , , cord-255936-hfa9w2dg 38 5 VLPs vlp NNS cord-255936-hfa9w2dg 38 6 were be VBD cord-255936-hfa9w2dg 38 7 purified purify VBN cord-255936-hfa9w2dg 38 8 from from IN cord-255936-hfa9w2dg 38 9 culture culture NN cord-255936-hfa9w2dg 38 10 supernatant supernatant VBN cord-255936-hfa9w2dg 38 11 by by IN cord-255936-hfa9w2dg 38 12 sucrose sucrose NN cord-255936-hfa9w2dg 38 13 gradient gradient NN cord-255936-hfa9w2dg 38 14 ultracentrifugation ultracentrifugation NN cord-255936-hfa9w2dg 38 15 . . . cord-255936-hfa9w2dg 39 1 Recombinant recombinant JJ cord-255936-hfa9w2dg 39 2 CP CP NNP cord-255936-hfa9w2dg 39 3 with with IN cord-255936-hfa9w2dg 39 4 a a DT cord-255936-hfa9w2dg 39 5 deletion deletion NN cord-255936-hfa9w2dg 39 6 of of IN cord-255936-hfa9w2dg 39 7 the the DT cord-255936-hfa9w2dg 39 8 N n CD cord-255936-hfa9w2dg 39 9 - - HYPH cord-255936-hfa9w2dg 39 10 terminal terminal JJ cord-255936-hfa9w2dg 39 11 region region NN cord-255936-hfa9w2dg 39 12 of of IN cord-255936-hfa9w2dg 39 13 the the DT cord-255936-hfa9w2dg 39 14 protein protein NN cord-255936-hfa9w2dg 39 15 ( ( -LRB- cord-255936-hfa9w2dg 39 16 delN deln CD cord-255936-hfa9w2dg 39 17 CP CP NNP cord-255936-hfa9w2dg 39 18 , , , cord-255936-hfa9w2dg 40 1 aa aa NNP cord-255936-hfa9w2dg 40 2 44e233 44e233 NNP cord-255936-hfa9w2dg 40 3 ) ) -RRB- cord-255936-hfa9w2dg 40 4 was be VBD cord-255936-hfa9w2dg 40 5 expressed express VBN cord-255936-hfa9w2dg 40 6 in in IN cord-255936-hfa9w2dg 40 7 Escherichia Escherichia NNP cord-255936-hfa9w2dg 40 8 coli coli NNS cord-255936-hfa9w2dg 40 9 and and CC cord-255936-hfa9w2dg 40 10 purified purify VBN cord-255936-hfa9w2dg 40 11 , , , cord-255936-hfa9w2dg 40 12 as as IN cord-255936-hfa9w2dg 40 13 described describe VBN cord-255936-hfa9w2dg 40 14 previously previously RB cord-255936-hfa9w2dg 40 15 [ [ -LRB- cord-255936-hfa9w2dg 40 16 12 12 CD cord-255936-hfa9w2dg 40 17 , , , cord-255936-hfa9w2dg 40 18 20 20 CD cord-255936-hfa9w2dg 40 19 , , , cord-255936-hfa9w2dg 40 20 21 21 CD cord-255936-hfa9w2dg 40 21 ] ] -RRB- cord-255936-hfa9w2dg 40 22 . . . cord-255936-hfa9w2dg 41 1 A a DT cord-255936-hfa9w2dg 41 2 synthetic synthetic JJ cord-255936-hfa9w2dg 41 3 CP CP NNP cord-255936-hfa9w2dg 41 4 169e180 169e180 CD cord-255936-hfa9w2dg 41 5 peptide peptide NN cord-255936-hfa9w2dg 41 6 ( ( -LRB- cord-255936-hfa9w2dg 41 7 STIDYFQPNNKRC STIDYFQPNNKRC NNP cord-255936-hfa9w2dg 41 8 ) ) -RRB- cord-255936-hfa9w2dg 41 9 was be VBD cord-255936-hfa9w2dg 41 10 chemically chemically RB cord-255936-hfa9w2dg 41 11 synthesized synthesize VBN cord-255936-hfa9w2dg 41 12 using use VBG cord-255936-hfa9w2dg 41 13 Fmoc Fmoc NNP cord-255936-hfa9w2dg 41 14 solid solid JJ cord-255936-hfa9w2dg 41 15 - - HYPH cord-255936-hfa9w2dg 41 16 phase phase NN cord-255936-hfa9w2dg 41 17 peptide peptide NN cord-255936-hfa9w2dg 41 18 synthesis synthesis NN cord-255936-hfa9w2dg 41 19 ( ( -LRB- cord-255936-hfa9w2dg 41 20 Peptron Peptron NNP cord-255936-hfa9w2dg 41 21 , , , cord-255936-hfa9w2dg 41 22 Inc. Inc. NNP cord-255936-hfa9w2dg 41 23 , , , cord-255936-hfa9w2dg 41 24 Daejeon Daejeon NNP cord-255936-hfa9w2dg 41 25 , , , cord-255936-hfa9w2dg 41 26 South South NNP cord-255936-hfa9w2dg 41 27 Korea Korea NNP cord-255936-hfa9w2dg 41 28 ) ) -RRB- cord-255936-hfa9w2dg 41 29 and and CC cord-255936-hfa9w2dg 41 30 conjugated conjugate VBN cord-255936-hfa9w2dg 41 31 to to IN cord-255936-hfa9w2dg 41 32 bovine bovine JJ cord-255936-hfa9w2dg 41 33 serum serum NN cord-255936-hfa9w2dg 41 34 albumin albumin NN cord-255936-hfa9w2dg 41 35 ( ( -LRB- cord-255936-hfa9w2dg 41 36 BSA BSA NNP cord-255936-hfa9w2dg 41 37 ) ) -RRB- cord-255936-hfa9w2dg 41 38 or or CC cord-255936-hfa9w2dg 41 39 keyhole keyhole NN cord-255936-hfa9w2dg 41 40 limpet limpet NNP cord-255936-hfa9w2dg 41 41 hemocyanin hemocyanin NNP cord-255936-hfa9w2dg 41 42 ( ( -LRB- cord-255936-hfa9w2dg 41 43 KLH KLH NNP cord-255936-hfa9w2dg 41 44 ) ) -RRB- cord-255936-hfa9w2dg 41 45 [ [ -LRB- cord-255936-hfa9w2dg 41 46 10 10 CD cord-255936-hfa9w2dg 41 47 ] ] -RRB- cord-255936-hfa9w2dg 41 48 . . . cord-255936-hfa9w2dg 42 1 White white JJ cord-255936-hfa9w2dg 42 2 leghorn leghorn NNP cord-255936-hfa9w2dg 42 3 chickens chicken NNS cord-255936-hfa9w2dg 42 4 were be VBD cord-255936-hfa9w2dg 42 5 immunized immunize VBN cord-255936-hfa9w2dg 42 6 with with IN cord-255936-hfa9w2dg 42 7 KLH KLH NNP cord-255936-hfa9w2dg 42 8 - - HYPH cord-255936-hfa9w2dg 42 9 conjugated conjugate VBN cord-255936-hfa9w2dg 42 10 CP CP NNP cord-255936-hfa9w2dg 42 11 169e180 169e180 CD cord-255936-hfa9w2dg 42 12 peptides peptide NNS cord-255936-hfa9w2dg 42 13 . . . cord-255936-hfa9w2dg 43 1 A a DT cord-255936-hfa9w2dg 43 2 phage phage NN cord-255936-hfa9w2dg 43 3 - - HYPH cord-255936-hfa9w2dg 43 4 displayed display VBN cord-255936-hfa9w2dg 43 5 chicken chicken NN cord-255936-hfa9w2dg 43 6 single single JJ cord-255936-hfa9w2dg 43 7 - - HYPH cord-255936-hfa9w2dg 43 8 chain chain NN cord-255936-hfa9w2dg 43 9 variable variable NN cord-255936-hfa9w2dg 43 10 fragment fragment NN cord-255936-hfa9w2dg 43 11 ( ( -LRB- cord-255936-hfa9w2dg 43 12 scFv scFv NNP cord-255936-hfa9w2dg 43 13 ) ) -RRB- cord-255936-hfa9w2dg 43 14 library library NN cord-255936-hfa9w2dg 43 15 was be VBD cord-255936-hfa9w2dg 43 16 constructed construct VBN cord-255936-hfa9w2dg 43 17 using use VBG cord-255936-hfa9w2dg 43 18 total total JJ cord-255936-hfa9w2dg 43 19 RNA RNA NNP cord-255936-hfa9w2dg 43 20 isolated isolate VBN cord-255936-hfa9w2dg 43 21 from from IN cord-255936-hfa9w2dg 43 22 the the DT cord-255936-hfa9w2dg 43 23 bone bone NN cord-255936-hfa9w2dg 43 24 marrow marrow NN cord-255936-hfa9w2dg 43 25 , , , cord-255936-hfa9w2dg 43 26 spleen spleen NNS cord-255936-hfa9w2dg 43 27 , , , cord-255936-hfa9w2dg 43 28 and and CC cord-255936-hfa9w2dg 43 29 bursa bursa NN cord-255936-hfa9w2dg 43 30 of of IN cord-255936-hfa9w2dg 43 31 Fabricius Fabricius NNP cord-255936-hfa9w2dg 43 32 of of IN cord-255936-hfa9w2dg 43 33 immunized immunize VBN cord-255936-hfa9w2dg 43 34 chickens chicken NNS cord-255936-hfa9w2dg 43 35 , , , cord-255936-hfa9w2dg 43 36 as as IN cord-255936-hfa9w2dg 43 37 previously previously RB cord-255936-hfa9w2dg 43 38 described describe VBN cord-255936-hfa9w2dg 43 39 [ [ -LRB- cord-255936-hfa9w2dg 43 40 22 22 CD cord-255936-hfa9w2dg 43 41 ] ] -RRB- cord-255936-hfa9w2dg 43 42 . . . cord-255936-hfa9w2dg 44 1 A a DT cord-255936-hfa9w2dg 44 2 positive positive JJ cord-255936-hfa9w2dg 44 3 clone clone NN cord-255936-hfa9w2dg 44 4 was be VBD cord-255936-hfa9w2dg 44 5 enriched enrich VBN cord-255936-hfa9w2dg 44 6 by by IN cord-255936-hfa9w2dg 44 7 biopanning biopanne VBG cord-255936-hfa9w2dg 44 8 and and CC cord-255936-hfa9w2dg 44 9 screened screen VBN cord-255936-hfa9w2dg 44 10 in in IN cord-255936-hfa9w2dg 44 11 a a DT cord-255936-hfa9w2dg 44 12 phage phage NN cord-255936-hfa9w2dg 44 13 enzyme enzyme NN cord-255936-hfa9w2dg 44 14 immunoassay immunoassay NN cord-255936-hfa9w2dg 44 15 , , , cord-255936-hfa9w2dg 44 16 as as IN cord-255936-hfa9w2dg 44 17 described describe VBN cord-255936-hfa9w2dg 44 18 previously previously RB cord-255936-hfa9w2dg 44 19 [ [ -LRB- cord-255936-hfa9w2dg 44 20 23 23 CD cord-255936-hfa9w2dg 44 21 , , , cord-255936-hfa9w2dg 44 22 24 24 CD cord-255936-hfa9w2dg 44 23 ] ] -RRB- cord-255936-hfa9w2dg 44 24 . . . cord-255936-hfa9w2dg 45 1 The the DT cord-255936-hfa9w2dg 45 2 gene gene NN cord-255936-hfa9w2dg 45 3 encoding encode VBG cord-255936-hfa9w2dg 45 4 the the DT cord-255936-hfa9w2dg 45 5 selected select VBN cord-255936-hfa9w2dg 45 6 scFv scfv NN cord-255936-hfa9w2dg 45 7 clone clone NN cord-255936-hfa9w2dg 45 8 was be VBD cord-255936-hfa9w2dg 45 9 subcloned subclone VBN cord-255936-hfa9w2dg 45 10 into into IN cord-255936-hfa9w2dg 45 11 a a DT cord-255936-hfa9w2dg 45 12 modified modify VBN cord-255936-hfa9w2dg 45 13 mammalian mammalian JJ cord-255936-hfa9w2dg 45 14 expression expression NN cord-255936-hfa9w2dg 45 15 vector vector NN cord-255936-hfa9w2dg 45 16 encoding encode VBG cord-255936-hfa9w2dg 45 17 the the DT cord-255936-hfa9w2dg 45 18 C c NN cord-255936-hfa9w2dg 45 19 k k NN cord-255936-hfa9w2dg 45 20 domain domain NN cord-255936-hfa9w2dg 45 21 of of IN cord-255936-hfa9w2dg 45 22 human human JJ cord-255936-hfa9w2dg 45 23 IgG igg NN cord-255936-hfa9w2dg 45 24 at at IN cord-255936-hfa9w2dg 45 25 the the DT cord-255936-hfa9w2dg 45 26 3 3 CD cord-255936-hfa9w2dg 45 27 0 0 CD cord-255936-hfa9w2dg 45 28 region region NN cord-255936-hfa9w2dg 45 29 [ [ -LRB- cord-255936-hfa9w2dg 45 30 25 25 CD cord-255936-hfa9w2dg 45 31 ] ] -RRB- cord-255936-hfa9w2dg 45 32 . . . cord-255936-hfa9w2dg 46 1 The the DT cord-255936-hfa9w2dg 46 2 scFv scFv NNP cord-255936-hfa9w2dg 46 3 - - HYPH cord-255936-hfa9w2dg 46 4 C C NNP cord-255936-hfa9w2dg 46 5 k k NN cord-255936-hfa9w2dg 46 6 fusion fusion NN cord-255936-hfa9w2dg 46 7 protein protein NN cord-255936-hfa9w2dg 46 8 ( ( -LRB- cord-255936-hfa9w2dg 46 9 anti anti JJ cord-255936-hfa9w2dg 46 10 - - JJ cord-255936-hfa9w2dg 46 11 CP cp JJ cord-255936-hfa9w2dg 46 12 169e180 169e180 CD cord-255936-hfa9w2dg 46 13 antibody antibody NN cord-255936-hfa9w2dg 46 14 ) ) -RRB- cord-255936-hfa9w2dg 46 15 was be VBD cord-255936-hfa9w2dg 46 16 purified purify VBN cord-255936-hfa9w2dg 46 17 from from IN cord-255936-hfa9w2dg 46 18 the the DT cord-255936-hfa9w2dg 46 19 culture culture NN cord-255936-hfa9w2dg 46 20 supernatants supernatant NNS cord-255936-hfa9w2dg 46 21 of of IN cord-255936-hfa9w2dg 46 22 transiently transiently RB cord-255936-hfa9w2dg 46 23 transfected transfecte VBN cord-255936-hfa9w2dg 46 24 HEK293F HEK293F NNP cord-255936-hfa9w2dg 46 25 cells cell NNS cord-255936-hfa9w2dg 46 26 using use VBG cord-255936-hfa9w2dg 46 27 KappaSelect KappaSelect NNP cord-255936-hfa9w2dg 46 28 resin resin NN cord-255936-hfa9w2dg 46 29 ( ( -LRB- cord-255936-hfa9w2dg 46 30 GE GE NNP cord-255936-hfa9w2dg 46 31 Fig Fig NNP cord-255936-hfa9w2dg 46 32 . . NNP cord-255936-hfa9w2dg 46 33 1 1 CD cord-255936-hfa9w2dg 46 34 . . . cord-255936-hfa9w2dg 47 1 A a DT cord-255936-hfa9w2dg 47 2 novel novel JJ cord-255936-hfa9w2dg 47 3 PCV2 pcv2 JJ cord-255936-hfa9w2dg 47 4 antibody antibody NN cord-255936-hfa9w2dg 47 5 is be VBZ cord-255936-hfa9w2dg 47 6 specific specific JJ cord-255936-hfa9w2dg 47 7 to to IN cord-255936-hfa9w2dg 47 8 the the DT cord-255936-hfa9w2dg 47 9 CP CP NNP cord-255936-hfa9w2dg 47 10 169e180 169e180 CD cord-255936-hfa9w2dg 47 11 epitope epitope NN cord-255936-hfa9w2dg 47 12 . . . cord-255936-hfa9w2dg 48 1 ( ( -LRB- cord-255936-hfa9w2dg 48 2 A a NN cord-255936-hfa9w2dg 48 3 ) ) -RRB- cord-255936-hfa9w2dg 48 4 The the DT cord-255936-hfa9w2dg 48 5 three three CD cord-255936-hfa9w2dg 48 6 - - HYPH cord-255936-hfa9w2dg 48 7 dimensional dimensional JJ cord-255936-hfa9w2dg 48 8 structure structure NN cord-255936-hfa9w2dg 48 9 of of IN cord-255936-hfa9w2dg 48 10 PCV2 pcv2 JJ cord-255936-hfa9w2dg 48 11 ( ( -LRB- cord-255936-hfa9w2dg 48 12 PDB PDB NNP cord-255936-hfa9w2dg 48 13 : : : cord-255936-hfa9w2dg 48 14 3JCI 3jci LS cord-255936-hfa9w2dg 48 15 ) ) -RRB- cord-255936-hfa9w2dg 48 16 was be VBD cord-255936-hfa9w2dg 48 17 visualized visualize VBN cord-255936-hfa9w2dg 48 18 by by IN cord-255936-hfa9w2dg 48 19 PyMOL PyMOL NNP cord-255936-hfa9w2dg 48 20 1.3 1.3 CD cord-255936-hfa9w2dg 48 21 . . . cord-255936-hfa9w2dg 49 1 One one CD cord-255936-hfa9w2dg 49 2 capsid capsid NN cord-255936-hfa9w2dg 49 3 protein protein NN cord-255936-hfa9w2dg 49 4 ( ( -LRB- cord-255936-hfa9w2dg 49 5 CP CP NNP cord-255936-hfa9w2dg 49 6 ) ) -RRB- cord-255936-hfa9w2dg 49 7 and and CC cord-255936-hfa9w2dg 49 8 the the DT cord-255936-hfa9w2dg 49 9 immunodominant immunodominant JJ cord-255936-hfa9w2dg 49 10 CP CP NNP cord-255936-hfa9w2dg 49 11 169e180 169e180 CD cord-255936-hfa9w2dg 49 12 decoy decoy NN cord-255936-hfa9w2dg 49 13 epitope epitope NN cord-255936-hfa9w2dg 49 14 are be VBP cord-255936-hfa9w2dg 49 15 marked mark VBN cord-255936-hfa9w2dg 49 16 in in IN cord-255936-hfa9w2dg 49 17 green green JJ cord-255936-hfa9w2dg 49 18 and and CC cord-255936-hfa9w2dg 49 19 red red JJ cord-255936-hfa9w2dg 49 20 , , , cord-255936-hfa9w2dg 49 21 respectively respectively RB cord-255936-hfa9w2dg 49 22 . . . cord-255936-hfa9w2dg 50 1 ( ( -LRB- cord-255936-hfa9w2dg 50 2 B b NN cord-255936-hfa9w2dg 50 3 ) ) -RRB- cord-255936-hfa9w2dg 50 4 Enzyme enzyme NN cord-255936-hfa9w2dg 50 5 immunoassay immunoassay NN cord-255936-hfa9w2dg 50 6 using use VBG cord-255936-hfa9w2dg 50 7 the the DT cord-255936-hfa9w2dg 50 8 anti anti JJ cord-255936-hfa9w2dg 50 9 - - JJ cord-255936-hfa9w2dg 50 10 CP cp JJ cord-255936-hfa9w2dg 50 11 169e180 169e180 CD cord-255936-hfa9w2dg 50 12 antibody antibody NN cord-255936-hfa9w2dg 50 13 . . . cord-255936-hfa9w2dg 51 1 The the DT cord-255936-hfa9w2dg 51 2 amount amount NN cord-255936-hfa9w2dg 51 3 of of IN cord-255936-hfa9w2dg 51 4 antibody antibody NN cord-255936-hfa9w2dg 51 5 bound bind VBN cord-255936-hfa9w2dg 51 6 to to IN cord-255936-hfa9w2dg 51 7 antigens antigen NNS cord-255936-hfa9w2dg 51 8 coated coat VBN cord-255936-hfa9w2dg 51 9 on on IN cord-255936-hfa9w2dg 51 10 microtiter microtiter NN cord-255936-hfa9w2dg 51 11 plate plate NN cord-255936-hfa9w2dg 51 12 was be VBD cord-255936-hfa9w2dg 51 13 detected detect VBN cord-255936-hfa9w2dg 51 14 by by IN cord-255936-hfa9w2dg 51 15 HRP HRP NNP cord-255936-hfa9w2dg 51 16 - - HYPH cord-255936-hfa9w2dg 51 17 conjugated conjugate VBN cord-255936-hfa9w2dg 51 18 goat goat NN cord-255936-hfa9w2dg 51 19 anti anti JJ cord-255936-hfa9w2dg 51 20 - - JJ cord-255936-hfa9w2dg 51 21 human human JJ cord-255936-hfa9w2dg 51 22 C c NN cord-255936-hfa9w2dg 51 23 k k NNP cord-255936-hfa9w2dg 51 24 antibody antibody NN cord-255936-hfa9w2dg 51 25 . . . cord-255936-hfa9w2dg 52 1 The the DT cord-255936-hfa9w2dg 52 2 results result NNS cord-255936-hfa9w2dg 52 3 are be VBP cord-255936-hfa9w2dg 52 4 the the DT cord-255936-hfa9w2dg 52 5 means mean NNS cord-255936-hfa9w2dg 52 6 ± ± NNP cord-255936-hfa9w2dg 52 7 standard standard JJ cord-255936-hfa9w2dg 52 8 deviations deviation NNS cord-255936-hfa9w2dg 52 9 from from IN cord-255936-hfa9w2dg 52 10 experiments experiment NNS cord-255936-hfa9w2dg 52 11 conducted conduct VBN cord-255936-hfa9w2dg 52 12 in in IN cord-255936-hfa9w2dg 52 13 triplicate triplicate NN cord-255936-hfa9w2dg 52 14 ; ; : cord-255936-hfa9w2dg 53 1 * * NFP cord-255936-hfa9w2dg 53 2 * * NFP cord-255936-hfa9w2dg 53 3 p p XX cord-255936-hfa9w2dg 53 4 < < XX cord-255936-hfa9w2dg 53 5 0.01 0.01 CD cord-255936-hfa9w2dg 53 6 , , , cord-255936-hfa9w2dg 54 1 * * NFP cord-255936-hfa9w2dg 54 2 * * NFP cord-255936-hfa9w2dg 54 3 * * NFP cord-255936-hfa9w2dg 54 4 p p XX cord-255936-hfa9w2dg 54 5 < < XX cord-255936-hfa9w2dg 54 6 0.001 0.001 CD cord-255936-hfa9w2dg 54 7 by by IN cord-255936-hfa9w2dg 54 8 two two CD cord-255936-hfa9w2dg 54 9 - - HYPH cord-255936-hfa9w2dg 54 10 tailed tailed JJ cord-255936-hfa9w2dg 54 11 unpaired unpaired NNP cord-255936-hfa9w2dg 54 12 Student Student NNP cord-255936-hfa9w2dg 54 13 's 's POS cord-255936-hfa9w2dg 54 14 t t NN cord-255936-hfa9w2dg 54 15 - - HYPH cord-255936-hfa9w2dg 54 16 test test NN cord-255936-hfa9w2dg 54 17 . . . cord-255936-hfa9w2dg 55 1 ( ( -LRB- cord-255936-hfa9w2dg 55 2 C C NNP cord-255936-hfa9w2dg 55 3 ) ) -RRB- cord-255936-hfa9w2dg 55 4 Immunoblot Immunoblot NNP cord-255936-hfa9w2dg 55 5 analysis analysis NN cord-255936-hfa9w2dg 55 6 using use VBG cord-255936-hfa9w2dg 55 7 the the DT cord-255936-hfa9w2dg 55 8 anti anti JJ cord-255936-hfa9w2dg 55 9 - - JJ cord-255936-hfa9w2dg 55 10 CP cp JJ cord-255936-hfa9w2dg 55 11 169e180 169e180 CD cord-255936-hfa9w2dg 55 12 antibody antibody NN cord-255936-hfa9w2dg 55 13 . . . cord-255936-hfa9w2dg 56 1 After after IN cord-255936-hfa9w2dg 56 2 the the DT cord-255936-hfa9w2dg 56 3 gel gel NN cord-255936-hfa9w2dg 56 4 electrophoresis electrophoresis NN cord-255936-hfa9w2dg 56 5 , , , cord-255936-hfa9w2dg 56 6 antigens antigen NNS cord-255936-hfa9w2dg 56 7 were be VBD cord-255936-hfa9w2dg 56 8 transfected transfecte VBN cord-255936-hfa9w2dg 56 9 to to IN cord-255936-hfa9w2dg 56 10 nitrocellulose nitrocellulose VBG cord-255936-hfa9w2dg 56 11 membrane membrane NN cord-255936-hfa9w2dg 56 12 . . . cord-255936-hfa9w2dg 57 1 The the DT cord-255936-hfa9w2dg 57 2 membrane membrane NN cord-255936-hfa9w2dg 57 3 was be VBD cord-255936-hfa9w2dg 57 4 probed probe VBN cord-255936-hfa9w2dg 57 5 with with IN cord-255936-hfa9w2dg 57 6 anti anti JJ cord-255936-hfa9w2dg 57 7 - - JJ cord-255936-hfa9w2dg 57 8 CP cp JJ cord-255936-hfa9w2dg 57 9 169e180 169e180 CD cord-255936-hfa9w2dg 57 10 antibody antibody NN cord-255936-hfa9w2dg 57 11 . . . cord-255936-hfa9w2dg 58 1 Healthcare Healthcare NNP cord-255936-hfa9w2dg 58 2 , , , cord-255936-hfa9w2dg 58 3 London London NNP cord-255936-hfa9w2dg 58 4 , , , cord-255936-hfa9w2dg 58 5 UK UK NNP cord-255936-hfa9w2dg 58 6 ) ) -RRB- cord-255936-hfa9w2dg 58 7 , , , cord-255936-hfa9w2dg 58 8 according accord VBG cord-255936-hfa9w2dg 58 9 to to IN cord-255936-hfa9w2dg 58 10 the the DT cord-255936-hfa9w2dg 58 11 manufacturer manufacturer NN cord-255936-hfa9w2dg 58 12 's 's POS cord-255936-hfa9w2dg 58 13 instructions instruction NNS cord-255936-hfa9w2dg 58 14 . . . cord-255936-hfa9w2dg 59 1 The the DT cord-255936-hfa9w2dg 59 2 wells well NNS cord-255936-hfa9w2dg 59 3 of of IN cord-255936-hfa9w2dg 59 4 a a DT cord-255936-hfa9w2dg 59 5 96-well 96-well CD cord-255936-hfa9w2dg 59 6 microtiter microtiter NN cord-255936-hfa9w2dg 59 7 plate plate NN cord-255936-hfa9w2dg 59 8 ( ( -LRB- cord-255936-hfa9w2dg 59 9 Corning Corning NNP cord-255936-hfa9w2dg 59 10 ) ) -RRB- cord-255936-hfa9w2dg 59 11 were be VBD cord-255936-hfa9w2dg 59 12 coated coat VBN cord-255936-hfa9w2dg 59 13 with with IN cord-255936-hfa9w2dg 59 14 BSA BSA NNP cord-255936-hfa9w2dg 59 15 - - HYPH cord-255936-hfa9w2dg 59 16 conjugated conjugate VBN cord-255936-hfa9w2dg 59 17 CP CP NNP cord-255936-hfa9w2dg 59 18 169e180 169e180 CD cord-255936-hfa9w2dg 59 19 peptides peptide NNS cord-255936-hfa9w2dg 59 20 , , , cord-255936-hfa9w2dg 59 21 delN deln SYM cord-255936-hfa9w2dg 59 22 CP CP NNP cord-255936-hfa9w2dg 59 23 , , , cord-255936-hfa9w2dg 59 24 and and CC cord-255936-hfa9w2dg 59 25 purified purify VBN cord-255936-hfa9w2dg 59 26 VLP VLP NNP cord-255936-hfa9w2dg 59 27 in in IN cord-255936-hfa9w2dg 59 28 coating coating NN cord-255936-hfa9w2dg 59 29 buffer buffer NN cord-255936-hfa9w2dg 59 30 ( ( -LRB- cord-255936-hfa9w2dg 59 31 0.1 0.1 CD cord-255936-hfa9w2dg 59 32 M M NNP cord-255936-hfa9w2dg 59 33 NaHCO NaHCO NNP cord-255936-hfa9w2dg 59 34 3 3 CD cord-255936-hfa9w2dg 59 35 , , , cord-255936-hfa9w2dg 59 36 pH pH NNP cord-255936-hfa9w2dg 59 37 8.6 8.6 CD cord-255936-hfa9w2dg 59 38 ) ) -RRB- cord-255936-hfa9w2dg 59 39 and and CC cord-255936-hfa9w2dg 59 40 then then RB cord-255936-hfa9w2dg 59 41 blocked block VBD cord-255936-hfa9w2dg 59 42 with with IN cord-255936-hfa9w2dg 59 43 3 3 CD cord-255936-hfa9w2dg 59 44 % % NN cord-255936-hfa9w2dg 59 45 ( ( -LRB- cord-255936-hfa9w2dg 59 46 w w NN cord-255936-hfa9w2dg 59 47 / / SYM cord-255936-hfa9w2dg 59 48 v v NN cord-255936-hfa9w2dg 59 49 ) ) -RRB- cord-255936-hfa9w2dg 59 50 BSA BSA NNP cord-255936-hfa9w2dg 59 51 in in IN cord-255936-hfa9w2dg 59 52 phosphate phosphate NN cord-255936-hfa9w2dg 59 53 - - HYPH cord-255936-hfa9w2dg 59 54 buffered buffer VBN cord-255936-hfa9w2dg 59 55 saline saline NN cord-255936-hfa9w2dg 59 56 ( ( -LRB- cord-255936-hfa9w2dg 59 57 PBS PBS NNP cord-255936-hfa9w2dg 59 58 ) ) -RRB- cord-255936-hfa9w2dg 59 59 . . . cord-255936-hfa9w2dg 60 1 After after IN cord-255936-hfa9w2dg 60 2 incubation incubation NN cord-255936-hfa9w2dg 60 3 with with IN cord-255936-hfa9w2dg 60 4 1 1 CD cord-255936-hfa9w2dg 60 5 mg mg NNP cord-255936-hfa9w2dg 60 6 / / SYM cord-255936-hfa9w2dg 60 7 mL mL NNP cord-255936-hfa9w2dg 60 8 of of IN cord-255936-hfa9w2dg 60 9 anti anti JJ cord-255936-hfa9w2dg 60 10 - - JJ cord-255936-hfa9w2dg 60 11 CP cp JJ cord-255936-hfa9w2dg 60 12 169e180 169e180 CD cord-255936-hfa9w2dg 60 13 antibody antibody NN cord-255936-hfa9w2dg 60 14 , , , cord-255936-hfa9w2dg 60 15 horseradish horseradish NN cord-255936-hfa9w2dg 60 16 peroxidase peroxidase NN cord-255936-hfa9w2dg 60 17 ( ( -LRB- cord-255936-hfa9w2dg 60 18 HRP)conjugated hrp)conjugate VBN cord-255936-hfa9w2dg 61 1 goat goat NNP cord-255936-hfa9w2dg 61 2 anti anti JJ cord-255936-hfa9w2dg 61 3 - - JJ cord-255936-hfa9w2dg 61 4 human human JJ cord-255936-hfa9w2dg 61 5 C c NN cord-255936-hfa9w2dg 61 6 k k NN cord-255936-hfa9w2dg 61 7 antibodies antibody NNS cord-255936-hfa9w2dg 61 8 ( ( -LRB- cord-255936-hfa9w2dg 61 9 Chemicon Chemicon NNP cord-255936-hfa9w2dg 61 10 - - HYPH cord-255936-hfa9w2dg 61 11 Millipore Millipore NNP cord-255936-hfa9w2dg 61 12 , , , cord-255936-hfa9w2dg 61 13 Billerica Billerica NNP cord-255936-hfa9w2dg 61 14 , , , cord-255936-hfa9w2dg 61 15 MA MA NNP cord-255936-hfa9w2dg 61 16 , , , cord-255936-hfa9w2dg 61 17 USA USA NNP cord-255936-hfa9w2dg 61 18 ) ) -RRB- cord-255936-hfa9w2dg 61 19 were be VBD cord-255936-hfa9w2dg 61 20 added add VBN cord-255936-hfa9w2dg 61 21 to to IN cord-255936-hfa9w2dg 61 22 each each DT cord-255936-hfa9w2dg 61 23 well well RB cord-255936-hfa9w2dg 61 24 . . . cord-255936-hfa9w2dg 62 1 After after IN cord-255936-hfa9w2dg 62 2 washing wash VBG cord-255936-hfa9w2dg 62 3 with with IN cord-255936-hfa9w2dg 62 4 0.05 0.05 CD cord-255936-hfa9w2dg 62 5 % % NN cord-255936-hfa9w2dg 62 6 ( ( -LRB- cord-255936-hfa9w2dg 62 7 v v NN cord-255936-hfa9w2dg 62 8 / / SYM cord-255936-hfa9w2dg 62 9 v v NN cord-255936-hfa9w2dg 62 10 ) ) -RRB- cord-255936-hfa9w2dg 62 11 Tween tween NN cord-255936-hfa9w2dg 62 12 20 20 CD cord-255936-hfa9w2dg 62 13 in in IN cord-255936-hfa9w2dg 62 14 PBS PBS NNP cord-255936-hfa9w2dg 62 15 ( ( -LRB- cord-255936-hfa9w2dg 62 16 PBST PBST NNP cord-255936-hfa9w2dg 62 17 ) ) -RRB- cord-255936-hfa9w2dg 62 18 , , , cord-255936-hfa9w2dg 62 19 3,3 3,3 CD cord-255936-hfa9w2dg 62 20 0 0 CD cord-255936-hfa9w2dg 62 21 , , , cord-255936-hfa9w2dg 62 22 5,5 5,5 CD cord-255936-hfa9w2dg 62 23 0 0 CD cord-255936-hfa9w2dg 63 1 -tetramethyl -tetramethyl NNP cord-255936-hfa9w2dg 63 2 benzidine benzidine NNP cord-255936-hfa9w2dg 63 3 ( ( -LRB- cord-255936-hfa9w2dg 63 4 TMB TMB NNP cord-255936-hfa9w2dg 63 5 ) ) -RRB- cord-255936-hfa9w2dg 64 1 ( ( -LRB- cord-255936-hfa9w2dg 64 2 GenDEPOT GenDEPOT NNP cord-255936-hfa9w2dg 64 3 , , , cord-255936-hfa9w2dg 64 4 Barker Barker NNP cord-255936-hfa9w2dg 64 5 , , , cord-255936-hfa9w2dg 64 6 TX TX NNP cord-255936-hfa9w2dg 64 7 , , , cord-255936-hfa9w2dg 64 8 USA USA NNP cord-255936-hfa9w2dg 64 9 ) ) -RRB- cord-255936-hfa9w2dg 64 10 substrate substrate NN cord-255936-hfa9w2dg 64 11 solution solution NN cord-255936-hfa9w2dg 64 12 was be VBD cord-255936-hfa9w2dg 64 13 added add VBN cord-255936-hfa9w2dg 64 14 and and CC cord-255936-hfa9w2dg 64 15 the the DT cord-255936-hfa9w2dg 64 16 absorbance absorbance NN cord-255936-hfa9w2dg 64 17 was be VBD cord-255936-hfa9w2dg 64 18 measured measure VBN cord-255936-hfa9w2dg 64 19 at at IN cord-255936-hfa9w2dg 64 20 650 650 CD cord-255936-hfa9w2dg 64 21 nm nm CD cord-255936-hfa9w2dg 64 22 with with IN cord-255936-hfa9w2dg 64 23 a a DT cord-255936-hfa9w2dg 64 24 Multiskan Multiskan NNP cord-255936-hfa9w2dg 64 25 Ascent Ascent NNP cord-255936-hfa9w2dg 64 26 microplate microplate NN cord-255936-hfa9w2dg 64 27 reader reader NN cord-255936-hfa9w2dg 64 28 ( ( -LRB- cord-255936-hfa9w2dg 64 29 LabSystems LabSystems NNP cord-255936-hfa9w2dg 64 30 , , , cord-255936-hfa9w2dg 64 31 Helsinki Helsinki NNP cord-255936-hfa9w2dg 64 32 , , , cord-255936-hfa9w2dg 64 33 Finland Finland NNP cord-255936-hfa9w2dg 64 34 ) ) -RRB- cord-255936-hfa9w2dg 64 35 [ [ -LRB- cord-255936-hfa9w2dg 64 36 26 26 CD cord-255936-hfa9w2dg 64 37 ] ] -RRB- cord-255936-hfa9w2dg 64 38 . . . cord-255936-hfa9w2dg 65 1 Dissolved dissolved JJ cord-255936-hfa9w2dg 65 2 proteins protein NNS cord-255936-hfa9w2dg 65 3 and and CC cord-255936-hfa9w2dg 65 4 BSA BSA NNP cord-255936-hfa9w2dg 65 5 - - HYPH cord-255936-hfa9w2dg 65 6 conjugated conjugate VBN cord-255936-hfa9w2dg 65 7 CP CP NNP cord-255936-hfa9w2dg 65 8 169e180 169e180 CD cord-255936-hfa9w2dg 65 9 peptides peptide NNS cord-255936-hfa9w2dg 65 10 were be VBD cord-255936-hfa9w2dg 65 11 boiled boil VBN cord-255936-hfa9w2dg 65 12 for for IN cord-255936-hfa9w2dg 65 13 10 10 CD cord-255936-hfa9w2dg 65 14 min min NN cord-255936-hfa9w2dg 65 15 in in IN cord-255936-hfa9w2dg 65 16 lithium lithium NN cord-255936-hfa9w2dg 65 17 dodecyl dodecyl NNP cord-255936-hfa9w2dg 65 18 sulfate sulfate NN cord-255936-hfa9w2dg 65 19 sample sample NN cord-255936-hfa9w2dg 65 20 buffer buffer NN cord-255936-hfa9w2dg 65 21 with with IN cord-255936-hfa9w2dg 65 22 reducing reduce VBG cord-255936-hfa9w2dg 65 23 agents agent NNS cord-255936-hfa9w2dg 65 24 ( ( -LRB- cord-255936-hfa9w2dg 65 25 Invitrogen Invitrogen NNP cord-255936-hfa9w2dg 65 26 ) ) -RRB- cord-255936-hfa9w2dg 65 27 . . . cord-255936-hfa9w2dg 66 1 The the DT cord-255936-hfa9w2dg 66 2 samples sample NNS cord-255936-hfa9w2dg 66 3 were be VBD cord-255936-hfa9w2dg 66 4 then then RB cord-255936-hfa9w2dg 66 5 electrophoretically electrophoretically RB cord-255936-hfa9w2dg 66 6 separated separate VBN cord-255936-hfa9w2dg 66 7 on on IN cord-255936-hfa9w2dg 66 8 a a DT cord-255936-hfa9w2dg 66 9 NuPage NuPage NNP cord-255936-hfa9w2dg 66 10 4e12 4e12 CD cord-255936-hfa9w2dg 66 11 % % NN cord-255936-hfa9w2dg 66 12 Bis Bis NNP cord-255936-hfa9w2dg 66 13 - - HYPH cord-255936-hfa9w2dg 66 14 Tris Tris NNP cord-255936-hfa9w2dg 66 15 gel gel NN cord-255936-hfa9w2dg 66 16 ( ( -LRB- cord-255936-hfa9w2dg 66 17 Invitrogen Invitrogen NNP cord-255936-hfa9w2dg 66 18 ) ) -RRB- cord-255936-hfa9w2dg 66 19 and and CC cord-255936-hfa9w2dg 66 20 transferred transfer VBN cord-255936-hfa9w2dg 66 21 to to IN cord-255936-hfa9w2dg 66 22 a a DT cord-255936-hfa9w2dg 66 23 nitrocellulose nitrocellulose JJ cord-255936-hfa9w2dg 66 24 membrane membrane NN cord-255936-hfa9w2dg 66 25 [ [ -LRB- cord-255936-hfa9w2dg 66 26 27 27 CD cord-255936-hfa9w2dg 66 27 ] ] -RRB- cord-255936-hfa9w2dg 66 28 . . . cord-255936-hfa9w2dg 67 1 After after IN cord-255936-hfa9w2dg 67 2 blocking block VBG cord-255936-hfa9w2dg 67 3 with with IN cord-255936-hfa9w2dg 67 4 5 5 CD cord-255936-hfa9w2dg 67 5 % % NN cord-255936-hfa9w2dg 67 6 ( ( -LRB- cord-255936-hfa9w2dg 67 7 w w NN cord-255936-hfa9w2dg 67 8 / / SYM cord-255936-hfa9w2dg 67 9 v v NN cord-255936-hfa9w2dg 67 10 ) ) -RRB- cord-255936-hfa9w2dg 67 11 skim skim VB cord-255936-hfa9w2dg 67 12 milk milk NN cord-255936-hfa9w2dg 67 13 in in IN cord-255936-hfa9w2dg 67 14 Tris Tris NNP cord-255936-hfa9w2dg 67 15 - - HYPH cord-255936-hfa9w2dg 67 16 buffered buffer VBN cord-255936-hfa9w2dg 67 17 saline saline NN cord-255936-hfa9w2dg 67 18 , , , cord-255936-hfa9w2dg 67 19 the the DT cord-255936-hfa9w2dg 67 20 membrane membrane NN cord-255936-hfa9w2dg 67 21 was be VBD cord-255936-hfa9w2dg 67 22 incubated incubate VBN cord-255936-hfa9w2dg 67 23 with with IN cord-255936-hfa9w2dg 67 24 1 1 CD cord-255936-hfa9w2dg 67 25 mg mg NNP cord-255936-hfa9w2dg 67 26 / / SYM cord-255936-hfa9w2dg 67 27 mL mL NNP cord-255936-hfa9w2dg 67 28 of of IN cord-255936-hfa9w2dg 67 29 anti anti JJ cord-255936-hfa9w2dg 67 30 - - JJ cord-255936-hfa9w2dg 67 31 CP cp JJ cord-255936-hfa9w2dg 67 32 169e180 169e180 CD cord-255936-hfa9w2dg 67 33 antibody antibody NN cord-255936-hfa9w2dg 67 34 and and CC cord-255936-hfa9w2dg 67 35 probed probe VBD cord-255936-hfa9w2dg 67 36 with with IN cord-255936-hfa9w2dg 67 37 HRP HRP NNP cord-255936-hfa9w2dg 67 38 - - HYPH cord-255936-hfa9w2dg 67 39 conjugated conjugate VBN cord-255936-hfa9w2dg 67 40 goat goat NN cord-255936-hfa9w2dg 67 41 anti anti JJ cord-255936-hfa9w2dg 67 42 - - JJ cord-255936-hfa9w2dg 67 43 human human JJ cord-255936-hfa9w2dg 67 44 C c NN cord-255936-hfa9w2dg 67 45 k k NN cord-255936-hfa9w2dg 67 46 antibodies antibody NNS cord-255936-hfa9w2dg 67 47 ( ( -LRB- cord-255936-hfa9w2dg 67 48 Chemicon Chemicon NNP cord-255936-hfa9w2dg 67 49 - - HYPH cord-255936-hfa9w2dg 67 50 Millipore Millipore NNP cord-255936-hfa9w2dg 67 51 ) ) -RRB- cord-255936-hfa9w2dg 67 52 . . . cord-255936-hfa9w2dg 68 1 The the DT cord-255936-hfa9w2dg 68 2 blot blot NN cord-255936-hfa9w2dg 68 3 was be VBD cord-255936-hfa9w2dg 68 4 visualized visualize VBN cord-255936-hfa9w2dg 68 5 using use VBG cord-255936-hfa9w2dg 68 6 SuperSignal SuperSignal NNP cord-255936-hfa9w2dg 68 7 West West NNP cord-255936-hfa9w2dg 68 8 Pico Pico NNP cord-255936-hfa9w2dg 68 9 chemiluminescent chemiluminescent NN cord-255936-hfa9w2dg 68 10 substrate substrate NN cord-255936-hfa9w2dg 68 11 ( ( -LRB- cord-255936-hfa9w2dg 68 12 Thermo Thermo NNP cord-255936-hfa9w2dg 68 13 Fisher Fisher NNP cord-255936-hfa9w2dg 68 14 Scientific Scientific NNP cord-255936-hfa9w2dg 68 15 , , , cord-255936-hfa9w2dg 68 16 IL IL NNP cord-255936-hfa9w2dg 68 17 , , , cord-255936-hfa9w2dg 68 18 USA USA NNP cord-255936-hfa9w2dg 68 19 ) ) -RRB- cord-255936-hfa9w2dg 68 20 . . . cord-255936-hfa9w2dg 69 1 All all DT cord-255936-hfa9w2dg 69 2 animal animal NN cord-255936-hfa9w2dg 69 3 experiments experiment NNS cord-255936-hfa9w2dg 69 4 were be VBD cord-255936-hfa9w2dg 69 5 performed perform VBN cord-255936-hfa9w2dg 69 6 under under IN cord-255936-hfa9w2dg 69 7 the the DT cord-255936-hfa9w2dg 69 8 approval approval NN cord-255936-hfa9w2dg 69 9 of of IN cord-255936-hfa9w2dg 69 10 the the DT cord-255936-hfa9w2dg 69 11 Institutional Institutional NNP cord-255936-hfa9w2dg 69 12 Animal Animal NNP cord-255936-hfa9w2dg 69 13 Care Care NNP cord-255936-hfa9w2dg 69 14 and and CC cord-255936-hfa9w2dg 69 15 Use Use NNP cord-255936-hfa9w2dg 69 16 Committee Committee NNP cord-255936-hfa9w2dg 69 17 of of IN cord-255936-hfa9w2dg 69 18 the the DT cord-255936-hfa9w2dg 69 19 BioPOA BioPOA NNP cord-255936-hfa9w2dg 69 20 ( ( -LRB- cord-255936-hfa9w2dg 69 21 permit permit VB cord-255936-hfa9w2dg 69 22 no no UH cord-255936-hfa9w2dg 69 23 . . . cord-255936-hfa9w2dg 70 1 BP-2015 BP-2015 NNP cord-255936-hfa9w2dg 70 2 - - HYPH cord-255936-hfa9w2dg 70 3 030 030 CD cord-255936-hfa9w2dg 70 4 - - HYPH cord-255936-hfa9w2dg 70 5 2 2 CD cord-255936-hfa9w2dg 70 6 ) ) -RRB- cord-255936-hfa9w2dg 70 7 . . . cord-255936-hfa9w2dg 71 1 After after IN cord-255936-hfa9w2dg 71 2 the the DT cord-255936-hfa9w2dg 71 3 collection collection NN cord-255936-hfa9w2dg 71 4 of of IN cord-255936-hfa9w2dg 71 5 sera sera NNP cord-255936-hfa9w2dg 71 6 from from IN cord-255936-hfa9w2dg 71 7 PCV2infected PCV2infected NNP cord-255936-hfa9w2dg 71 8 pigs pig NNS cord-255936-hfa9w2dg 71 9 and and CC cord-255936-hfa9w2dg 71 10 pigs pig NNS cord-255936-hfa9w2dg 71 11 with with IN cord-255936-hfa9w2dg 71 12 no no DT cord-255936-hfa9w2dg 71 13 infection infection NN cord-255936-hfa9w2dg 71 14 history history NN cord-255936-hfa9w2dg 71 15 , , , cord-255936-hfa9w2dg 71 16 the the DT cord-255936-hfa9w2dg 71 17 competition competition NN cord-255936-hfa9w2dg 71 18 enzyme enzyme NN cord-255936-hfa9w2dg 71 19 immunoassay immunoassay NN cord-255936-hfa9w2dg 71 20 was be VBD cord-255936-hfa9w2dg 71 21 performed perform VBN cord-255936-hfa9w2dg 71 22 as as IN cord-255936-hfa9w2dg 71 23 described describe VBN cord-255936-hfa9w2dg 71 24 previously previously RB cord-255936-hfa9w2dg 71 25 with with IN cord-255936-hfa9w2dg 71 26 the the DT cord-255936-hfa9w2dg 71 27 following follow VBG cord-255936-hfa9w2dg 71 28 appropriate appropriate JJ cord-255936-hfa9w2dg 71 29 modifications modification NNS cord-255936-hfa9w2dg 71 30 [ [ -LRB- cord-255936-hfa9w2dg 71 31 25 25 CD cord-255936-hfa9w2dg 71 32 ] ] -RRB- cord-255936-hfa9w2dg 71 33 . . . cord-255936-hfa9w2dg 72 1 After after IN cord-255936-hfa9w2dg 72 2 pre pre JJ cord-255936-hfa9w2dg 72 3 - - JJ cord-255936-hfa9w2dg 72 4 incubating incubating JJ cord-255936-hfa9w2dg 72 5 non non JJ cord-255936-hfa9w2dg 72 6 - - JJ cord-255936-hfa9w2dg 72 7 infected infected JJ cord-255936-hfa9w2dg 72 8 and and CC cord-255936-hfa9w2dg 72 9 infected infect VBN cord-255936-hfa9w2dg 72 10 porcine porcine JJ cord-255936-hfa9w2dg 72 11 sera sera FW cord-255936-hfa9w2dg 72 12 ( ( -LRB- cord-255936-hfa9w2dg 72 13 n n NNP cord-255936-hfa9w2dg 72 14 ¼ ¼ NNP cord-255936-hfa9w2dg 72 15 3 3 CD cord-255936-hfa9w2dg 72 16 / / SYM cord-255936-hfa9w2dg 72 17 group group NN cord-255936-hfa9w2dg 72 18 ) ) -RRB- cord-255936-hfa9w2dg 72 19 with with IN cord-255936-hfa9w2dg 72 20 serially serially RB cord-255936-hfa9w2dg 72 21 diluted dilute VBN cord-255936-hfa9w2dg 72 22 anti anti JJ cord-255936-hfa9w2dg 72 23 - - JJ cord-255936-hfa9w2dg 72 24 CP cp JJ cord-255936-hfa9w2dg 72 25 169e180 169e180 CD cord-255936-hfa9w2dg 72 26 antibody antibody NN cord-255936-hfa9w2dg 72 27 , , , cord-255936-hfa9w2dg 72 28 the the DT cord-255936-hfa9w2dg 72 29 mixtures mixture NNS cord-255936-hfa9w2dg 72 30 were be VBD cord-255936-hfa9w2dg 72 31 added add VBN cord-255936-hfa9w2dg 72 32 to to IN cord-255936-hfa9w2dg 72 33 microtiter microtiter NNP cord-255936-hfa9w2dg 72 34 plate plate NN cord-255936-hfa9w2dg 72 35 coated coat VBN cord-255936-hfa9w2dg 72 36 with with IN cord-255936-hfa9w2dg 72 37 BSA BSA NNP cord-255936-hfa9w2dg 72 38 - - HYPH cord-255936-hfa9w2dg 72 39 conjugated conjugate VBN cord-255936-hfa9w2dg 72 40 CP CP NNP cord-255936-hfa9w2dg 72 41 169e180 169e180 CD cord-255936-hfa9w2dg 72 42 peptide peptide NN cord-255936-hfa9w2dg 72 43 . . . cord-255936-hfa9w2dg 73 1 An an DT cord-255936-hfa9w2dg 73 2 irrelevant irrelevant JJ cord-255936-hfa9w2dg 73 3 scFv scfv NN cord-255936-hfa9w2dg 73 4 fused fuse VBN cord-255936-hfa9w2dg 73 5 with with IN cord-255936-hfa9w2dg 73 6 a a DT cord-255936-hfa9w2dg 73 7 C c NN cord-255936-hfa9w2dg 73 8 k k NN cord-255936-hfa9w2dg 73 9 domain domain NN cord-255936-hfa9w2dg 73 10 was be VBD cord-255936-hfa9w2dg 73 11 used use VBN cord-255936-hfa9w2dg 73 12 as as IN cord-255936-hfa9w2dg 73 13 an an DT cord-255936-hfa9w2dg 73 14 isotypematched isotypematched JJ cord-255936-hfa9w2dg 73 15 control control NN cord-255936-hfa9w2dg 73 16 . . . cord-255936-hfa9w2dg 74 1 The the DT cord-255936-hfa9w2dg 74 2 amount amount NN cord-255936-hfa9w2dg 74 3 of of IN cord-255936-hfa9w2dg 74 4 bound bind VBN cord-255936-hfa9w2dg 74 5 porcine porcine JJ cord-255936-hfa9w2dg 74 6 IgG igg NN cord-255936-hfa9w2dg 74 7 was be VBD cord-255936-hfa9w2dg 74 8 determined determine VBN cord-255936-hfa9w2dg 74 9 by by IN cord-255936-hfa9w2dg 74 10 HRP HRP NNP cord-255936-hfa9w2dg 74 11 - - HYPH cord-255936-hfa9w2dg 74 12 conjugated conjugate VBN cord-255936-hfa9w2dg 74 13 goat goat NN cord-255936-hfa9w2dg 74 14 anti anti JJ cord-255936-hfa9w2dg 74 15 - - JJ cord-255936-hfa9w2dg 74 16 porcine porcine JJ cord-255936-hfa9w2dg 74 17 IgG igg NN cord-255936-hfa9w2dg 74 18 antibodies antibody NNS cord-255936-hfa9w2dg 74 19 ( ( -LRB- cord-255936-hfa9w2dg 74 20 Santa Santa NNP cord-255936-hfa9w2dg 74 21 Cruz Cruz NNP cord-255936-hfa9w2dg 74 22 Biotechnology Biotechnology NNP cord-255936-hfa9w2dg 74 23 , , , cord-255936-hfa9w2dg 74 24 TX TX NNP cord-255936-hfa9w2dg 74 25 , , , cord-255936-hfa9w2dg 74 26 USA USA NNP cord-255936-hfa9w2dg 74 27 ) ) -RRB- cord-255936-hfa9w2dg 74 28 and and CC cord-255936-hfa9w2dg 74 29 TMB TMB NNP cord-255936-hfa9w2dg 74 30 was be VBD cord-255936-hfa9w2dg 74 31 used use VBN cord-255936-hfa9w2dg 74 32 for for IN cord-255936-hfa9w2dg 74 33 the the DT cord-255936-hfa9w2dg 74 34 substrate substrate NN cord-255936-hfa9w2dg 74 35 of of IN cord-255936-hfa9w2dg 74 36 HRP HRP NNP cord-255936-hfa9w2dg 74 37 . . . cord-255936-hfa9w2dg 75 1 We -PRON- PRP cord-255936-hfa9w2dg 75 2 randomly randomly RB cord-255936-hfa9w2dg 75 3 designated designate VBD cord-255936-hfa9w2dg 75 4 two two CD cord-255936-hfa9w2dg 75 5 of of IN cord-255936-hfa9w2dg 75 6 out out IN cord-255936-hfa9w2dg 75 7 nine nine CD cord-255936-hfa9w2dg 75 8 commercial commercial JJ cord-255936-hfa9w2dg 75 9 vaccines vaccine NNS cord-255936-hfa9w2dg 75 10 ( ( -LRB- cord-255936-hfa9w2dg 75 11 The the DT cord-255936-hfa9w2dg 75 12 level level NN cord-255936-hfa9w2dg 75 13 of of IN cord-255936-hfa9w2dg 75 14 total total JJ cord-255936-hfa9w2dg 75 15 PCV2 pcv2 JJ cord-255936-hfa9w2dg 75 16 antigen antigen NN cord-255936-hfa9w2dg 75 17 in in IN cord-255936-hfa9w2dg 75 18 prepared prepared JJ cord-255936-hfa9w2dg 75 19 VLP VLP NNP cord-255936-hfa9w2dg 75 20 , , , cord-255936-hfa9w2dg 75 21 delN delN NNP cord-255936-hfa9w2dg 75 22 CP CP NNP cord-255936-hfa9w2dg 75 23 , , , cord-255936-hfa9w2dg 75 24 and and CC cord-255936-hfa9w2dg 75 25 the the DT cord-255936-hfa9w2dg 75 26 two two CD cord-255936-hfa9w2dg 75 27 commercial commercial JJ cord-255936-hfa9w2dg 75 28 vaccines vaccine NNS cord-255936-hfa9w2dg 75 29 was be VBD cord-255936-hfa9w2dg 75 30 measured measure VBN cord-255936-hfa9w2dg 75 31 by by IN cord-255936-hfa9w2dg 75 32 enzyme enzyme NN cord-255936-hfa9w2dg 75 33 immunoassay immunoassay NN cord-255936-hfa9w2dg 75 34 using use VBG cord-255936-hfa9w2dg 75 35 an an DT cord-255936-hfa9w2dg 75 36 anti anti JJ cord-255936-hfa9w2dg 75 37 - - JJ cord-255936-hfa9w2dg 75 38 PCV2 pcv2 JJ cord-255936-hfa9w2dg 75 39 polyclonal polyclonal JJ cord-255936-hfa9w2dg 75 40 antibody antibody NN cord-255936-hfa9w2dg 75 41 ( ( -LRB- cord-255936-hfa9w2dg 75 42 Veterinary Veterinary NNP cord-255936-hfa9w2dg 75 43 Diagnostic Diagnostic NNP cord-255936-hfa9w2dg 75 44 Laboratory Laboratory NNP cord-255936-hfa9w2dg 75 45 , , , cord-255936-hfa9w2dg 75 46 Iowa Iowa NNP cord-255936-hfa9w2dg 75 47 State State NNP cord-255936-hfa9w2dg 75 48 University University NNP cord-255936-hfa9w2dg 75 49 , , , cord-255936-hfa9w2dg 75 50 Iowa Iowa NNP cord-255936-hfa9w2dg 75 51 , , , cord-255936-hfa9w2dg 75 52 USA USA NNP cord-255936-hfa9w2dg 75 53 ) ) -RRB- cord-255936-hfa9w2dg 75 54 [ [ -LRB- cord-255936-hfa9w2dg 75 55 28 28 CD cord-255936-hfa9w2dg 75 56 ] ] -RRB- cord-255936-hfa9w2dg 75 57 and and CC cord-255936-hfa9w2dg 75 58 HRP HRP NNP cord-255936-hfa9w2dg 75 59 - - HYPH cord-255936-hfa9w2dg 75 60 conjugated conjugate VBN cord-255936-hfa9w2dg 75 61 goat goat NN cord-255936-hfa9w2dg 75 62 anti anti JJ cord-255936-hfa9w2dg 75 63 - - JJ cord-255936-hfa9w2dg 75 64 rabbit rabbit JJ cord-255936-hfa9w2dg 75 65 IgG igg NN cord-255936-hfa9w2dg 75 66 antibodies antibody NNS cord-255936-hfa9w2dg 75 67 ( ( -LRB- cord-255936-hfa9w2dg 75 68 Bethyl Bethyl NNP cord-255936-hfa9w2dg 75 69 Laboratories Laboratories NNP cord-255936-hfa9w2dg 75 70 , , , cord-255936-hfa9w2dg 75 71 Montgomery Montgomery NNP cord-255936-hfa9w2dg 75 72 , , , cord-255936-hfa9w2dg 75 73 TX TX NNP cord-255936-hfa9w2dg 75 74 , , , cord-255936-hfa9w2dg 75 75 USA USA NNP cord-255936-hfa9w2dg 75 76 ) ) -RRB- cord-255936-hfa9w2dg 75 77 , , , cord-255936-hfa9w2dg 75 78 as as IN cord-255936-hfa9w2dg 75 79 described describe VBN cord-255936-hfa9w2dg 75 80 previously previously RB cord-255936-hfa9w2dg 75 81 . . . cord-255936-hfa9w2dg 76 1 To to TO cord-255936-hfa9w2dg 76 2 monitor monitor VB cord-255936-hfa9w2dg 76 3 the the DT cord-255936-hfa9w2dg 76 4 CP CP NNP cord-255936-hfa9w2dg 76 5 169e180 169e180 CD cord-255936-hfa9w2dg 76 6 epitope epitope NN cord-255936-hfa9w2dg 76 7 levels level NNS cord-255936-hfa9w2dg 76 8 , , , cord-255936-hfa9w2dg 76 9 we -PRON- PRP cord-255936-hfa9w2dg 76 10 performed perform VBD cord-255936-hfa9w2dg 76 11 the the DT cord-255936-hfa9w2dg 76 12 enzyme enzyme NN cord-255936-hfa9w2dg 76 13 immunoassay immunoassay NN cord-255936-hfa9w2dg 76 14 using use VBG cord-255936-hfa9w2dg 76 15 the the DT cord-255936-hfa9w2dg 76 16 anti anti JJ cord-255936-hfa9w2dg 76 17 - - JJ cord-255936-hfa9w2dg 76 18 CP cp JJ cord-255936-hfa9w2dg 76 19 169e180 169e180 CD cord-255936-hfa9w2dg 76 20 antibody antibody NN cord-255936-hfa9w2dg 76 21 and and CC cord-255936-hfa9w2dg 76 22 HRPconjugated hrpconjugated JJ cord-255936-hfa9w2dg 76 23 goat goat NN cord-255936-hfa9w2dg 76 24 anti anti JJ cord-255936-hfa9w2dg 76 25 - - JJ cord-255936-hfa9w2dg 76 26 human human JJ cord-255936-hfa9w2dg 76 27 C c NN cord-255936-hfa9w2dg 76 28 k k NN cord-255936-hfa9w2dg 76 29 antibodies antibody NNS cord-255936-hfa9w2dg 76 30 ( ( -LRB- cord-255936-hfa9w2dg 76 31 Chemicon Chemicon NNP cord-255936-hfa9w2dg 76 32 - - HYPH cord-255936-hfa9w2dg 76 33 Millipore Millipore NNP cord-255936-hfa9w2dg 76 34 ) ) -RRB- cord-255936-hfa9w2dg 76 35 , , , cord-255936-hfa9w2dg 76 36 as as IN cord-255936-hfa9w2dg 76 37 previously previously RB cord-255936-hfa9w2dg 76 38 described describe VBN cord-255936-hfa9w2dg 76 39 . . . cord-255936-hfa9w2dg 77 1 Twenty twenty CD cord-255936-hfa9w2dg 77 2 guinea guinea NN cord-255936-hfa9w2dg 77 3 pigs pig NNS cord-255936-hfa9w2dg 77 4 were be VBD cord-255936-hfa9w2dg 77 5 randomly randomly RB cord-255936-hfa9w2dg 77 6 divided divide VBN cord-255936-hfa9w2dg 77 7 into into IN cord-255936-hfa9w2dg 77 8 four four CD cord-255936-hfa9w2dg 77 9 groups group NNS cord-255936-hfa9w2dg 77 10 ( ( -LRB- cord-255936-hfa9w2dg 77 11 n n NNP cord-255936-hfa9w2dg 77 12 ¼ ¼ NNP cord-255936-hfa9w2dg 77 13 5 5 CD cord-255936-hfa9w2dg 77 14 / / SYM cord-255936-hfa9w2dg 77 15 group group NN cord-255936-hfa9w2dg 77 16 ) ) -RRB- cord-255936-hfa9w2dg 77 17 . . . cord-255936-hfa9w2dg 78 1 Each each DT cord-255936-hfa9w2dg 78 2 guinea guinea NN cord-255936-hfa9w2dg 78 3 pig pig NN cord-255936-hfa9w2dg 78 4 was be VBD cord-255936-hfa9w2dg 78 5 immunized immunize VBN cord-255936-hfa9w2dg 78 6 intradermally intradermally RB cord-255936-hfa9w2dg 78 7 with with IN cord-255936-hfa9w2dg 78 8 200 200 CD cord-255936-hfa9w2dg 78 9 mL ml NN cord-255936-hfa9w2dg 78 10 of of IN cord-255936-hfa9w2dg 78 11 VLP VLP NNP cord-255936-hfa9w2dg 78 12 , , , cord-255936-hfa9w2dg 78 13 delN delN NNP cord-255936-hfa9w2dg 78 14 CP CP NNP cord-255936-hfa9w2dg 78 15 , , , cord-255936-hfa9w2dg 78 16 or or CC cord-255936-hfa9w2dg 78 17 commercial commercial JJ cord-255936-hfa9w2dg 78 18 vaccine vaccine NN cord-255936-hfa9w2dg 78 19 . . . cord-255936-hfa9w2dg 79 1 Four four CD cord-255936-hfa9w2dg 79 2 weeks week NNS cord-255936-hfa9w2dg 79 3 after after IN cord-255936-hfa9w2dg 79 4 immunization immunization NN cord-255936-hfa9w2dg 79 5 , , , cord-255936-hfa9w2dg 79 6 whole whole JJ cord-255936-hfa9w2dg 79 7 blood blood NN cord-255936-hfa9w2dg 79 8 was be VBD cord-255936-hfa9w2dg 79 9 collected collect VBN cord-255936-hfa9w2dg 79 10 and and CC cord-255936-hfa9w2dg 79 11 sera sera NNP cord-255936-hfa9w2dg 79 12 were be VBD cord-255936-hfa9w2dg 79 13 prepared prepare VBN cord-255936-hfa9w2dg 79 14 . . . cord-255936-hfa9w2dg 80 1 An an DT cord-255936-hfa9w2dg 80 2 indirect indirect JJ cord-255936-hfa9w2dg 80 3 fluorescence fluorescence NN cord-255936-hfa9w2dg 80 4 assay assay NN cord-255936-hfa9w2dg 80 5 ( ( -LRB- cord-255936-hfa9w2dg 80 6 IFA IFA NNP cord-255936-hfa9w2dg 80 7 ) ) -RRB- cord-255936-hfa9w2dg 80 8 was be VBD cord-255936-hfa9w2dg 80 9 performed perform VBN cord-255936-hfa9w2dg 80 10 using use VBG cord-255936-hfa9w2dg 80 11 the the DT cord-255936-hfa9w2dg 80 12 sera sera NNP cord-255936-hfa9w2dg 80 13 and and CC cord-255936-hfa9w2dg 80 14 PCV2-infected PCV2-infected NNP cord-255936-hfa9w2dg 80 15 PK-15 PK-15 NNP cord-255936-hfa9w2dg 80 16 cells cell NNS cord-255936-hfa9w2dg 80 17 , , , cord-255936-hfa9w2dg 80 18 as as IN cord-255936-hfa9w2dg 80 19 previously previously RB cord-255936-hfa9w2dg 80 20 described describe VBN cord-255936-hfa9w2dg 80 21 [ [ -LRB- cord-255936-hfa9w2dg 80 22 18 18 CD cord-255936-hfa9w2dg 80 23 , , , cord-255936-hfa9w2dg 80 24 29 29 CD cord-255936-hfa9w2dg 80 25 ] ] -RRB- cord-255936-hfa9w2dg 80 26 . . . cord-255936-hfa9w2dg 81 1 To to TO cord-255936-hfa9w2dg 81 2 monitor monitor VB cord-255936-hfa9w2dg 81 3 the the DT cord-255936-hfa9w2dg 81 4 reactivity reactivity NN cord-255936-hfa9w2dg 81 5 of of IN cord-255936-hfa9w2dg 81 6 sera sera NNP cord-255936-hfa9w2dg 81 7 to to IN cord-255936-hfa9w2dg 81 8 the the DT cord-255936-hfa9w2dg 81 9 CP CP NNP cord-255936-hfa9w2dg 81 10 169e180 169e180 CD cord-255936-hfa9w2dg 81 11 epitope epitope NN cord-255936-hfa9w2dg 81 12 , , , cord-255936-hfa9w2dg 81 13 enzyme enzyme NN cord-255936-hfa9w2dg 81 14 immunoassays immunoassays NNP cord-255936-hfa9w2dg 81 15 , , , cord-255936-hfa9w2dg 81 16 using use VBG cord-255936-hfa9w2dg 81 17 BSA BSA NNP cord-255936-hfa9w2dg 81 18 - - HYPH cord-255936-hfa9w2dg 81 19 conjugated conjugate VBN cord-255936-hfa9w2dg 81 20 CP CP NNP cord-255936-hfa9w2dg 81 21 169e180 169e180 CD cord-255936-hfa9w2dg 81 22 peptides peptide NNS cord-255936-hfa9w2dg 81 23 and and CC cord-255936-hfa9w2dg 81 24 HRPconjugated hrpconjugated JJ cord-255936-hfa9w2dg 81 25 anti anti JJ cord-255936-hfa9w2dg 81 26 - - JJ cord-255936-hfa9w2dg 81 27 guinea guinea JJ cord-255936-hfa9w2dg 81 28 pig pig NN cord-255936-hfa9w2dg 81 29 IgG IgG NNP cord-255936-hfa9w2dg 81 30 antibodies antibody NNS cord-255936-hfa9w2dg 81 31 ( ( -LRB- cord-255936-hfa9w2dg 81 32 Bethyl Bethyl NNP cord-255936-hfa9w2dg 81 33 Laboratories Laboratories NNPS cord-255936-hfa9w2dg 81 34 ) ) -RRB- cord-255936-hfa9w2dg 81 35 , , , cord-255936-hfa9w2dg 81 36 were be VBD cord-255936-hfa9w2dg 81 37 performed perform VBN cord-255936-hfa9w2dg 81 38 as as IN cord-255936-hfa9w2dg 81 39 described describe VBN cord-255936-hfa9w2dg 81 40 previously previously RB cord-255936-hfa9w2dg 81 41 . . . cord-255936-hfa9w2dg 82 1 We -PRON- PRP cord-255936-hfa9w2dg 82 2 performed perform VBD cord-255936-hfa9w2dg 82 3 serum serum NN cord-255936-hfa9w2dg 82 4 virus virus NN cord-255936-hfa9w2dg 82 5 neutralization neutralization NN cord-255936-hfa9w2dg 82 6 assays assay NNS cord-255936-hfa9w2dg 82 7 using use VBG cord-255936-hfa9w2dg 82 8 PK-15 PK-15 NNP cord-255936-hfa9w2dg 82 9 cells cell NNS cord-255936-hfa9w2dg 82 10 , , , cord-255936-hfa9w2dg 82 11 as as IN cord-255936-hfa9w2dg 82 12 described describe VBN cord-255936-hfa9w2dg 82 13 previously previously RB cord-255936-hfa9w2dg 82 14 [ [ -LRB- cord-255936-hfa9w2dg 82 15 30 30 CD cord-255936-hfa9w2dg 82 16 , , , cord-255936-hfa9w2dg 82 17 31 31 CD cord-255936-hfa9w2dg 82 18 ] ] -RRB- cord-255936-hfa9w2dg 82 19 . . . cord-255936-hfa9w2dg 83 1 Statistical statistical JJ cord-255936-hfa9w2dg 83 2 analysis analysis NN cord-255936-hfa9w2dg 83 3 was be VBD cord-255936-hfa9w2dg 83 4 conducted conduct VBN cord-255936-hfa9w2dg 83 5 using use VBG cord-255936-hfa9w2dg 83 6 GraphPad GraphPad NNP cord-255936-hfa9w2dg 83 7 Prism Prism NNP cord-255936-hfa9w2dg 83 8 ( ( -LRB- cord-255936-hfa9w2dg 83 9 v5.0 v5.0 NN cord-255936-hfa9w2dg 83 10 ; ; : cord-255936-hfa9w2dg 83 11 GraphPad GraphPad NNP cord-255936-hfa9w2dg 83 12 Software Software NNP cord-255936-hfa9w2dg 83 13 Inc. Inc. NNP cord-255936-hfa9w2dg 83 14 , , , cord-255936-hfa9w2dg 83 15 San San NNP cord-255936-hfa9w2dg 83 16 Diego Diego NNP cord-255936-hfa9w2dg 83 17 , , , cord-255936-hfa9w2dg 83 18 CA CA NNP cord-255936-hfa9w2dg 83 19 , , , cord-255936-hfa9w2dg 83 20 USA USA NNP cord-255936-hfa9w2dg 83 21 ) ) -RRB- cord-255936-hfa9w2dg 83 22 . . . cord-255936-hfa9w2dg 84 1 Results result NNS cord-255936-hfa9w2dg 84 2 are be VBP cord-255936-hfa9w2dg 84 3 expressed express VBN cord-255936-hfa9w2dg 84 4 as as IN cord-255936-hfa9w2dg 84 5 means mean NNS cord-255936-hfa9w2dg 84 6 and and CC cord-255936-hfa9w2dg 84 7 standard standard JJ cord-255936-hfa9w2dg 84 8 deviations deviation NNS cord-255936-hfa9w2dg 84 9 of of IN cord-255936-hfa9w2dg 84 10 the the DT cord-255936-hfa9w2dg 84 11 indicated indicate VBN cord-255936-hfa9w2dg 84 12 number number NN cord-255936-hfa9w2dg 84 13 of of IN cord-255936-hfa9w2dg 84 14 independent independent JJ cord-255936-hfa9w2dg 84 15 measurements measurement NNS cord-255936-hfa9w2dg 84 16 . . . cord-255936-hfa9w2dg 85 1 Statistical statistical JJ cord-255936-hfa9w2dg 85 2 significance significance NN cord-255936-hfa9w2dg 85 3 was be VBD cord-255936-hfa9w2dg 85 4 determined determine VBN cord-255936-hfa9w2dg 85 5 using use VBG cord-255936-hfa9w2dg 85 6 two two CD cord-255936-hfa9w2dg 85 7 - - HYPH cord-255936-hfa9w2dg 85 8 tailed tailed JJ cord-255936-hfa9w2dg 85 9 unpaired unpaired NNP cord-255936-hfa9w2dg 85 10 Student Student NNP cord-255936-hfa9w2dg 85 11 's 's POS cord-255936-hfa9w2dg 85 12 t t NN cord-255936-hfa9w2dg 85 13 tests test NNS cord-255936-hfa9w2dg 85 14 and and CC cord-255936-hfa9w2dg 85 15 non non JJ cord-255936-hfa9w2dg 85 16 - - JJ cord-255936-hfa9w2dg 85 17 parametric parametric JJ cord-255936-hfa9w2dg 85 18 Mann Mann NNP cord-255936-hfa9w2dg 85 19 - - HYPH cord-255936-hfa9w2dg 85 20 Whitney Whitney NNP cord-255936-hfa9w2dg 85 21 's 's POS cord-255936-hfa9w2dg 85 22 u u NNP cord-255936-hfa9w2dg 85 23 test test NN cord-255936-hfa9w2dg 85 24 . . . cord-255936-hfa9w2dg 86 1 Two two CD cord-255936-hfa9w2dg 86 2 - - HYPH cord-255936-hfa9w2dg 86 3 way way NN cord-255936-hfa9w2dg 86 4 analysis analysis NN cord-255936-hfa9w2dg 86 5 of of IN cord-255936-hfa9w2dg 86 6 variance variance NN cord-255936-hfa9w2dg 86 7 ( ( -LRB- cord-255936-hfa9w2dg 86 8 ANOVA ANOVA NNP cord-255936-hfa9w2dg 86 9 ) ) -RRB- cord-255936-hfa9w2dg 86 10 was be VBD cord-255936-hfa9w2dg 86 11 used use VBN cord-255936-hfa9w2dg 86 12 to to TO cord-255936-hfa9w2dg 86 13 analyze analyze VB cord-255936-hfa9w2dg 86 14 the the DT cord-255936-hfa9w2dg 86 15 relationship relationship NN cord-255936-hfa9w2dg 86 16 between between IN cord-255936-hfa9w2dg 86 17 the the DT cord-255936-hfa9w2dg 86 18 two two CD cord-255936-hfa9w2dg 86 19 independent independent JJ cord-255936-hfa9w2dg 86 20 factors factor NNS cord-255936-hfa9w2dg 86 21 . . . cord-255936-hfa9w2dg 87 1 For for IN cord-255936-hfa9w2dg 87 2 all all DT cord-255936-hfa9w2dg 87 3 statistical statistical JJ cord-255936-hfa9w2dg 87 4 analyses analysis NNS cord-255936-hfa9w2dg 87 5 , , , cord-255936-hfa9w2dg 87 6 p p NN cord-255936-hfa9w2dg 87 7 values value NNS cord-255936-hfa9w2dg 87 8 of of IN cord-255936-hfa9w2dg 87 9 < < XX cord-255936-hfa9w2dg 87 10 0.05 0.05 CD cord-255936-hfa9w2dg 87 11 were be VBD cord-255936-hfa9w2dg 87 12 considered consider VBN cord-255936-hfa9w2dg 87 13 statistically statistically RB cord-255936-hfa9w2dg 87 14 significant significant JJ cord-255936-hfa9w2dg 87 15 . . . cord-255936-hfa9w2dg 88 1 A a DT cord-255936-hfa9w2dg 88 2 phage phage NN cord-255936-hfa9w2dg 88 3 display display NN cord-255936-hfa9w2dg 88 4 library library NN cord-255936-hfa9w2dg 88 5 of of IN cord-255936-hfa9w2dg 88 6 combinatorial combinatorial JJ cord-255936-hfa9w2dg 88 7 scFv scFv NNP cord-255936-hfa9w2dg 88 8 was be VBD cord-255936-hfa9w2dg 88 9 prepared prepare VBN cord-255936-hfa9w2dg 88 10 using use VBG cord-255936-hfa9w2dg 88 11 chickens chicken NNS cord-255936-hfa9w2dg 88 12 immunized immunize VBN cord-255936-hfa9w2dg 88 13 with with IN cord-255936-hfa9w2dg 88 14 KLH KLH NNP cord-255936-hfa9w2dg 88 15 - - HYPH cord-255936-hfa9w2dg 88 16 conjugated conjugate VBN cord-255936-hfa9w2dg 88 17 CP CP NNP cord-255936-hfa9w2dg 88 18 169e180 169e180 CD cord-255936-hfa9w2dg 88 19 peptides peptide NNS cord-255936-hfa9w2dg 88 20 . . . cord-255936-hfa9w2dg 89 1 The the DT cord-255936-hfa9w2dg 89 2 complexity complexity NN cord-255936-hfa9w2dg 89 3 of of IN cord-255936-hfa9w2dg 89 4 the the DT cord-255936-hfa9w2dg 89 5 library library NN cord-255936-hfa9w2dg 89 6 exceeded exceed VBD cord-255936-hfa9w2dg 89 7 1.2 1.2 CD cord-255936-hfa9w2dg 89 8 Â â CD cord-255936-hfa9w2dg 89 9 10 10 CD cord-255936-hfa9w2dg 89 10 9 9 CD cord-255936-hfa9w2dg 89 11 . . . cord-255936-hfa9w2dg 90 1 To to TO cord-255936-hfa9w2dg 90 2 enrich enrich VB cord-255936-hfa9w2dg 90 3 for for IN cord-255936-hfa9w2dg 90 4 scFv scfv JJ cord-255936-hfa9w2dg 90 5 clones clone NNS cord-255936-hfa9w2dg 90 6 with with IN cord-255936-hfa9w2dg 90 7 specific specific JJ cord-255936-hfa9w2dg 90 8 reactivity reactivity NN cord-255936-hfa9w2dg 90 9 to to IN cord-255936-hfa9w2dg 90 10 the the DT cord-255936-hfa9w2dg 90 11 CP CP NNP cord-255936-hfa9w2dg 90 12 169e180 169e180 CD cord-255936-hfa9w2dg 90 13 epitope epitope NN cord-255936-hfa9w2dg 90 14 , , , cord-255936-hfa9w2dg 90 15 six six CD cord-255936-hfa9w2dg 90 16 total total JJ cord-255936-hfa9w2dg 90 17 rounds round NNS cord-255936-hfa9w2dg 90 18 of of IN cord-255936-hfa9w2dg 90 19 biopanning biopanning NN cord-255936-hfa9w2dg 90 20 were be VBD cord-255936-hfa9w2dg 90 21 performed perform VBN cord-255936-hfa9w2dg 90 22 . . . cord-255936-hfa9w2dg 91 1 For for IN cord-255936-hfa9w2dg 91 2 each each DT cord-255936-hfa9w2dg 91 3 round round NN cord-255936-hfa9w2dg 91 4 of of IN cord-255936-hfa9w2dg 91 5 biopanning biopanning NN cord-255936-hfa9w2dg 91 6 , , , cord-255936-hfa9w2dg 91 7 monomeric monomeric JJ cord-255936-hfa9w2dg 91 8 delN deln SYM cord-255936-hfa9w2dg 91 9 CP CP NNP cord-255936-hfa9w2dg 91 10 and and CC cord-255936-hfa9w2dg 91 11 CP CP NNP cord-255936-hfa9w2dg 91 12 169e180 169e180 CD cord-255936-hfa9w2dg 91 13 peptides peptide NNS cord-255936-hfa9w2dg 91 14 were be VBD cord-255936-hfa9w2dg 91 15 alternately alternately RB cord-255936-hfa9w2dg 91 16 used use VBN cord-255936-hfa9w2dg 91 17 as as IN cord-255936-hfa9w2dg 91 18 antigens antigen NNS cord-255936-hfa9w2dg 91 19 . . . cord-255936-hfa9w2dg 92 1 The the DT cord-255936-hfa9w2dg 92 2 N n CD cord-255936-hfa9w2dg 92 3 terminus terminus NN cord-255936-hfa9w2dg 92 4 of of IN cord-255936-hfa9w2dg 92 5 CP CP NNP cord-255936-hfa9w2dg 92 6 plays play VBZ cord-255936-hfa9w2dg 92 7 a a DT cord-255936-hfa9w2dg 92 8 key key JJ cord-255936-hfa9w2dg 92 9 role role NN cord-255936-hfa9w2dg 92 10 in in IN cord-255936-hfa9w2dg 92 11 the the DT cord-255936-hfa9w2dg 92 12 assembly assembly NN cord-255936-hfa9w2dg 92 13 of of IN cord-255936-hfa9w2dg 92 14 the the DT cord-255936-hfa9w2dg 92 15 VLP VLP NNP cord-255936-hfa9w2dg 92 16 structure structure NN cord-255936-hfa9w2dg 92 17 . . . cord-255936-hfa9w2dg 93 1 Thus thus RB cord-255936-hfa9w2dg 93 2 , , , cord-255936-hfa9w2dg 93 3 deletion deletion NN cord-255936-hfa9w2dg 93 4 of of IN cord-255936-hfa9w2dg 93 5 the the DT cord-255936-hfa9w2dg 93 6 N n CD cord-255936-hfa9w2dg 93 7 - - HYPH cord-255936-hfa9w2dg 93 8 terminal terminal NN cord-255936-hfa9w2dg 93 9 region region NN cord-255936-hfa9w2dg 93 10 exposes expose VBZ cord-255936-hfa9w2dg 93 11 the the DT cord-255936-hfa9w2dg 93 12 CP CP NNP cord-255936-hfa9w2dg 93 13 169e180 169e180 CD cord-255936-hfa9w2dg 93 14 epitope epitope NN cord-255936-hfa9w2dg 93 15 ( ( -LRB- cord-255936-hfa9w2dg 93 16 del del FW cord-255936-hfa9w2dg 93 17 CP CP NNP cord-255936-hfa9w2dg 93 18 ) ) -RRB- cord-255936-hfa9w2dg 93 19 . . . cord-255936-hfa9w2dg 94 1 After after IN cord-255936-hfa9w2dg 94 2 biopanning biopanne VBG cord-255936-hfa9w2dg 94 3 , , , cord-255936-hfa9w2dg 94 4 the the DT cord-255936-hfa9w2dg 94 5 reactivity reactivity NN cord-255936-hfa9w2dg 94 6 of of IN cord-255936-hfa9w2dg 94 7 individual individual JJ cord-255936-hfa9w2dg 94 8 clones clone NNS cord-255936-hfa9w2dg 94 9 to to IN cord-255936-hfa9w2dg 94 10 CP CP NNP cord-255936-hfa9w2dg 94 11 169e180 169e180 CD cord-255936-hfa9w2dg 94 12 peptide peptide NN cord-255936-hfa9w2dg 94 13 , , , cord-255936-hfa9w2dg 94 14 delN deln SYM cord-255936-hfa9w2dg 94 15 CP CP NNP cord-255936-hfa9w2dg 94 16 , , , cord-255936-hfa9w2dg 94 17 and and CC cord-255936-hfa9w2dg 94 18 PCV2 PCV2 NNP cord-255936-hfa9w2dg 94 19 VLPs vlp NNS cord-255936-hfa9w2dg 94 20 was be VBD cord-255936-hfa9w2dg 94 21 tested test VBN cord-255936-hfa9w2dg 94 22 by by IN cord-255936-hfa9w2dg 94 23 phage phage NN cord-255936-hfa9w2dg 94 24 enzyme enzyme NN cord-255936-hfa9w2dg 94 25 immunoassay immunoassay NN cord-255936-hfa9w2dg 94 26 . . . cord-255936-hfa9w2dg 95 1 A a DT cord-255936-hfa9w2dg 95 2 clone clone NN cord-255936-hfa9w2dg 95 3 was be VBD cord-255936-hfa9w2dg 95 4 selected select VBN cord-255936-hfa9w2dg 95 5 , , , cord-255936-hfa9w2dg 95 6 and and CC cord-255936-hfa9w2dg 95 7 its -PRON- PRP$ cord-255936-hfa9w2dg 95 8 recombinant recombinant JJ cord-255936-hfa9w2dg 95 9 scFv scFv NNP cord-255936-hfa9w2dg 95 10 - - HYPH cord-255936-hfa9w2dg 95 11 C C NNP cord-255936-hfa9w2dg 95 12 k k NNP cord-255936-hfa9w2dg 95 13 fusion fusion NN cord-255936-hfa9w2dg 95 14 protein protein NN cord-255936-hfa9w2dg 95 15 prepared prepare VBD cord-255936-hfa9w2dg 95 16 using use VBG cord-255936-hfa9w2dg 95 17 a a DT cord-255936-hfa9w2dg 95 18 mammalian mammalian JJ cord-255936-hfa9w2dg 95 19 expression expression NN cord-255936-hfa9w2dg 95 20 system system NN cord-255936-hfa9w2dg 95 21 . . . cord-255936-hfa9w2dg 96 1 The the DT cord-255936-hfa9w2dg 96 2 recombinant recombinant JJ cord-255936-hfa9w2dg 96 3 scFv scFv NNP cord-255936-hfa9w2dg 96 4 - - HYPH cord-255936-hfa9w2dg 96 5 C C NNP cord-255936-hfa9w2dg 96 6 k k NNP cord-255936-hfa9w2dg 96 7 fusion fusion NN cord-255936-hfa9w2dg 96 8 protein protein NN cord-255936-hfa9w2dg 96 9 reacted react VBD cord-255936-hfa9w2dg 96 10 to to IN cord-255936-hfa9w2dg 96 11 CP CP NNP cord-255936-hfa9w2dg 96 12 169e180 169e180 CD cord-255936-hfa9w2dg 96 13 peptide peptide NN cord-255936-hfa9w2dg 96 14 and and CC cord-255936-hfa9w2dg 96 15 delN deln SYM cord-255936-hfa9w2dg 96 16 CP CP NNP cord-255936-hfa9w2dg 96 17 , , , cord-255936-hfa9w2dg 96 18 but but CC cord-255936-hfa9w2dg 96 19 not not RB cord-255936-hfa9w2dg 96 20 to to IN cord-255936-hfa9w2dg 96 21 VLPs vlp NNS cord-255936-hfa9w2dg 96 22 ( ( -LRB- cord-255936-hfa9w2dg 96 23 Fig fig NN cord-255936-hfa9w2dg 96 24 . . . cord-255936-hfa9w2dg 96 25 1B 1b CD cord-255936-hfa9w2dg 96 26 ) ) -RRB- cord-255936-hfa9w2dg 96 27 . . . cord-255936-hfa9w2dg 97 1 In in IN cord-255936-hfa9w2dg 97 2 immunoblot immunoblot NNP cord-255936-hfa9w2dg 97 3 analysis analysis NN cord-255936-hfa9w2dg 97 4 , , , cord-255936-hfa9w2dg 97 5 the the DT cord-255936-hfa9w2dg 97 6 recombinant recombinant JJ cord-255936-hfa9w2dg 97 7 scFv scFv NNP cord-255936-hfa9w2dg 97 8 - - HYPH cord-255936-hfa9w2dg 97 9 C C NNP cord-255936-hfa9w2dg 97 10 k k NNP cord-255936-hfa9w2dg 97 11 fusion fusion NN cord-255936-hfa9w2dg 97 12 protein protein NN cord-255936-hfa9w2dg 97 13 was be VBD cord-255936-hfa9w2dg 97 14 reactive reactive JJ cord-255936-hfa9w2dg 97 15 not not RB cord-255936-hfa9w2dg 97 16 only only RB cord-255936-hfa9w2dg 97 17 to to IN cord-255936-hfa9w2dg 97 18 the the DT cord-255936-hfa9w2dg 97 19 CP CP NNP cord-255936-hfa9w2dg 97 20 169e180 169e180 CD cord-255936-hfa9w2dg 97 21 peptide peptide NN cord-255936-hfa9w2dg 97 22 and and CC cord-255936-hfa9w2dg 97 23 delN deln SYM cord-255936-hfa9w2dg 97 24 CP CP NNP cord-255936-hfa9w2dg 97 25 , , , cord-255936-hfa9w2dg 97 26 but but CC cord-255936-hfa9w2dg 97 27 also also RB cord-255936-hfa9w2dg 97 28 to to IN cord-255936-hfa9w2dg 97 29 VLPs vlp NNS cord-255936-hfa9w2dg 97 30 , , , cord-255936-hfa9w2dg 97 31 because because IN cord-255936-hfa9w2dg 97 32 the the DT cord-255936-hfa9w2dg 97 33 CP CP NNP cord-255936-hfa9w2dg 97 34 169e180 169e180 CD cord-255936-hfa9w2dg 97 35 epitope epitope NN cord-255936-hfa9w2dg 97 36 on on IN cord-255936-hfa9w2dg 97 37 VLP VLP NNP cord-255936-hfa9w2dg 97 38 was be VBD cord-255936-hfa9w2dg 97 39 exposed expose VBN cord-255936-hfa9w2dg 97 40 by by IN cord-255936-hfa9w2dg 97 41 the the DT cord-255936-hfa9w2dg 97 42 denaturation denaturation NN cord-255936-hfa9w2dg 97 43 that that WDT cord-255936-hfa9w2dg 97 44 occurs occur VBZ cord-255936-hfa9w2dg 97 45 during during IN cord-255936-hfa9w2dg 97 46 sample sample NN cord-255936-hfa9w2dg 97 47 preparation preparation NN cord-255936-hfa9w2dg 97 48 ( ( -LRB- cord-255936-hfa9w2dg 97 49 Fig Fig NNP cord-255936-hfa9w2dg 97 50 . . . cord-255936-hfa9w2dg 97 51 1C 1C NNP cord-255936-hfa9w2dg 97 52 ) ) -RRB- cord-255936-hfa9w2dg 97 53 . . . cord-255936-hfa9w2dg 98 1 Although although IN cord-255936-hfa9w2dg 98 2 the the DT cord-255936-hfa9w2dg 98 3 CP CP NNP cord-255936-hfa9w2dg 98 4 169e180 169e180 CD cord-255936-hfa9w2dg 98 5 segment segment NN cord-255936-hfa9w2dg 98 6 is be VBZ cord-255936-hfa9w2dg 98 7 too too RB cord-255936-hfa9w2dg 98 8 short short JJ cord-255936-hfa9w2dg 98 9 to to TO cord-255936-hfa9w2dg 98 10 harbor harbor VB cord-255936-hfa9w2dg 98 11 more more JJR cord-255936-hfa9w2dg 98 12 than than IN cord-255936-hfa9w2dg 98 13 one one CD cord-255936-hfa9w2dg 98 14 antigenic antigenic JJ cord-255936-hfa9w2dg 98 15 determinant determinant NN cord-255936-hfa9w2dg 98 16 , , , cord-255936-hfa9w2dg 98 17 we -PRON- PRP cord-255936-hfa9w2dg 98 18 further further RB cord-255936-hfa9w2dg 98 19 confirmed confirm VBD cord-255936-hfa9w2dg 98 20 a a DT cord-255936-hfa9w2dg 98 21 number number NN cord-255936-hfa9w2dg 98 22 of of IN cord-255936-hfa9w2dg 98 23 antigenic antigenic JJ cord-255936-hfa9w2dg 98 24 determinants determinant NNS cord-255936-hfa9w2dg 98 25 using use VBG cord-255936-hfa9w2dg 98 26 the the DT cord-255936-hfa9w2dg 98 27 polyclonal polyclonal JJ cord-255936-hfa9w2dg 98 28 sera sera NNP cord-255936-hfa9w2dg 98 29 of of IN cord-255936-hfa9w2dg 98 30 pigs pig NNS cord-255936-hfa9w2dg 98 31 infected infect VBN cord-255936-hfa9w2dg 98 32 with with IN cord-255936-hfa9w2dg 98 33 PCV2 PCV2 NNP cord-255936-hfa9w2dg 98 34 . . . cord-255936-hfa9w2dg 99 1 In in IN cord-255936-hfa9w2dg 99 2 a a DT cord-255936-hfa9w2dg 99 3 competition competition NN cord-255936-hfa9w2dg 99 4 enzyme enzyme NN cord-255936-hfa9w2dg 99 5 immunoassay immunoassay NN cord-255936-hfa9w2dg 99 6 employing employ VBG cord-255936-hfa9w2dg 99 7 a a DT cord-255936-hfa9w2dg 99 8 microtiter microtiter NN cord-255936-hfa9w2dg 99 9 plate plate NN cord-255936-hfa9w2dg 99 10 coated coat VBN cord-255936-hfa9w2dg 99 11 with with IN cord-255936-hfa9w2dg 99 12 BSA BSA NNP cord-255936-hfa9w2dg 99 13 - - HYPH cord-255936-hfa9w2dg 99 14 conjugated conjugate VBN cord-255936-hfa9w2dg 99 15 CP CP NNP cord-255936-hfa9w2dg 99 16 169e180 169e180 CD cord-255936-hfa9w2dg 99 17 peptide peptide NN cord-255936-hfa9w2dg 99 18 and and CC cord-255936-hfa9w2dg 99 19 HRP HRP NNP cord-255936-hfa9w2dg 99 20 - - HYPH cord-255936-hfa9w2dg 99 21 conjugated conjugate VBN cord-255936-hfa9w2dg 99 22 anti anti JJ cord-255936-hfa9w2dg 99 23 - - JJ cord-255936-hfa9w2dg 99 24 porcine porcine JJ cord-255936-hfa9w2dg 99 25 IgG igg NN cord-255936-hfa9w2dg 99 26 antibody antibody NN cord-255936-hfa9w2dg 99 27 , , , cord-255936-hfa9w2dg 99 28 the the DT cord-255936-hfa9w2dg 99 29 recombinant recombinant JJ cord-255936-hfa9w2dg 99 30 anti anti JJ cord-255936-hfa9w2dg 99 31 - - JJ cord-255936-hfa9w2dg 99 32 CP cp JJ cord-255936-hfa9w2dg 99 33 169e180 169e180 CD cord-255936-hfa9w2dg 99 34 antibody antibody NN cord-255936-hfa9w2dg 99 35 almost almost RB cord-255936-hfa9w2dg 99 36 completely completely RB cord-255936-hfa9w2dg 99 37 inhibited inhibit VBD cord-255936-hfa9w2dg 99 38 the the DT cord-255936-hfa9w2dg 99 39 binding binding NN cord-255936-hfa9w2dg 99 40 of of IN cord-255936-hfa9w2dg 99 41 naturally naturally RB cord-255936-hfa9w2dg 99 42 occurring occur VBG cord-255936-hfa9w2dg 99 43 anti anti JJ cord-255936-hfa9w2dg 99 44 - - JJ cord-255936-hfa9w2dg 99 45 CP cp JJ cord-255936-hfa9w2dg 99 46 169e180 169e180 CD cord-255936-hfa9w2dg 99 47 antibody antibody NN cord-255936-hfa9w2dg 99 48 to to IN cord-255936-hfa9w2dg 99 49 the the DT cord-255936-hfa9w2dg 99 50 peptide peptide NN cord-255936-hfa9w2dg 99 51 in in IN cord-255936-hfa9w2dg 99 52 a a DT cord-255936-hfa9w2dg 99 53 PCV2-infected pcv2-infected JJ cord-255936-hfa9w2dg 99 54 pig pig NN cord-255936-hfa9w2dg 99 55 's 's POS cord-255936-hfa9w2dg 99 56 sera sera NNP cord-255936-hfa9w2dg 99 57 ( ( -LRB- cord-255936-hfa9w2dg 99 58 Fig Fig NNP cord-255936-hfa9w2dg 99 59 . . NNP cord-255936-hfa9w2dg 99 60 2 2 CD cord-255936-hfa9w2dg 99 61 ) ) -RRB- cord-255936-hfa9w2dg 99 62 . . . cord-255936-hfa9w2dg 100 1 From from IN cord-255936-hfa9w2dg 100 2 these these DT cord-255936-hfa9w2dg 100 3 results result NNS cord-255936-hfa9w2dg 100 4 , , , cord-255936-hfa9w2dg 100 5 we -PRON- PRP cord-255936-hfa9w2dg 100 6 concluded conclude VBD cord-255936-hfa9w2dg 100 7 that that IN cord-255936-hfa9w2dg 100 8 there there EX cord-255936-hfa9w2dg 100 9 is be VBZ cord-255936-hfa9w2dg 100 10 only only RB cord-255936-hfa9w2dg 100 11 one one CD cord-255936-hfa9w2dg 100 12 antigenic antigenic JJ cord-255936-hfa9w2dg 100 13 determinant determinant NN cord-255936-hfa9w2dg 100 14 in in IN cord-255936-hfa9w2dg 100 15 CP CP NNP cord-255936-hfa9w2dg 100 16 169e180 169e180 CD cord-255936-hfa9w2dg 100 17 residues residue NNS cord-255936-hfa9w2dg 100 18 . . . cord-255936-hfa9w2dg 101 1 The the DT cord-255936-hfa9w2dg 101 2 relative relative JJ cord-255936-hfa9w2dg 101 3 amount amount NN cord-255936-hfa9w2dg 101 4 of of IN cord-255936-hfa9w2dg 101 5 PCV2 pcv2 JJ cord-255936-hfa9w2dg 101 6 antigen antigen NN cord-255936-hfa9w2dg 101 7 in in IN cord-255936-hfa9w2dg 101 8 two two CD cord-255936-hfa9w2dg 101 9 commercially commercially RB cord-255936-hfa9w2dg 101 10 available available JJ cord-255936-hfa9w2dg 101 11 vaccines vaccine NNS cord-255936-hfa9w2dg 101 12 was be VBD cord-255936-hfa9w2dg 101 13 determined determine VBN cord-255936-hfa9w2dg 101 14 in in IN cord-255936-hfa9w2dg 101 15 an an DT cord-255936-hfa9w2dg 101 16 enzyme enzyme NN cord-255936-hfa9w2dg 101 17 immunoassay immunoassay NN cord-255936-hfa9w2dg 101 18 , , , cord-255936-hfa9w2dg 101 19 using use VBG cord-255936-hfa9w2dg 101 20 purified purify VBN cord-255936-hfa9w2dg 101 21 VLP VLP NNP cord-255936-hfa9w2dg 101 22 and and CC cord-255936-hfa9w2dg 101 23 the the DT cord-255936-hfa9w2dg 101 24 recombinant recombinant JJ cord-255936-hfa9w2dg 101 25 delN deln JJ cord-255936-hfa9w2dg 101 26 CP cp NN cord-255936-hfa9w2dg 101 27 protein protein NN cord-255936-hfa9w2dg 101 28 . . . cord-255936-hfa9w2dg 102 1 The the DT cord-255936-hfa9w2dg 102 2 amount amount NN cord-255936-hfa9w2dg 102 3 of of IN cord-255936-hfa9w2dg 102 4 coated coated JJ cord-255936-hfa9w2dg 102 5 antigen antigen NN cord-255936-hfa9w2dg 102 6 was be VBD cord-255936-hfa9w2dg 102 7 then then RB cord-255936-hfa9w2dg 102 8 determined determine VBN cord-255936-hfa9w2dg 102 9 by by IN cord-255936-hfa9w2dg 102 10 anti anti JJ cord-255936-hfa9w2dg 102 11 - - JJ cord-255936-hfa9w2dg 102 12 PCV2 pcv2 JJ cord-255936-hfa9w2dg 102 13 polyclonal polyclonal JJ cord-255936-hfa9w2dg 102 14 antibody antibody NN cord-255936-hfa9w2dg 102 15 . . . cord-255936-hfa9w2dg 103 1 Because because IN cord-255936-hfa9w2dg 103 2 the the DT cord-255936-hfa9w2dg 103 3 quantity quantity NN cord-255936-hfa9w2dg 103 4 of of IN cord-255936-hfa9w2dg 103 5 bound bind VBN cord-255936-hfa9w2dg 103 6 antibody antibody NN cord-255936-hfa9w2dg 103 7 was be VBD cord-255936-hfa9w2dg 103 8 not not RB cord-255936-hfa9w2dg 103 9 statistically statistically RB cord-255936-hfa9w2dg 103 10 different different JJ cord-255936-hfa9w2dg 103 11 between between IN cord-255936-hfa9w2dg 103 12 the the DT cord-255936-hfa9w2dg 103 13 vaccines vaccine NNS cord-255936-hfa9w2dg 103 14 , , , cord-255936-hfa9w2dg 103 15 we -PRON- PRP cord-255936-hfa9w2dg 103 16 concluded conclude VBD cord-255936-hfa9w2dg 103 17 that that IN cord-255936-hfa9w2dg 103 18 they -PRON- PRP cord-255936-hfa9w2dg 103 19 contained contain VBD cord-255936-hfa9w2dg 103 20 similar similar JJ cord-255936-hfa9w2dg 103 21 amounts amount NNS cord-255936-hfa9w2dg 103 22 of of IN cord-255936-hfa9w2dg 103 23 the the DT cord-255936-hfa9w2dg 103 24 recombinant recombinant JJ cord-255936-hfa9w2dg 103 25 CP cp NN cord-255936-hfa9w2dg 103 26 ( ( -LRB- cord-255936-hfa9w2dg 103 27 Fig Fig NNP cord-255936-hfa9w2dg 103 28 . . . cord-255936-hfa9w2dg 103 29 3A 3A NNP cord-255936-hfa9w2dg 103 30 ) ) -RRB- cord-255936-hfa9w2dg 103 31 . . . cord-255936-hfa9w2dg 104 1 We -PRON- PRP cord-255936-hfa9w2dg 104 2 then then RB cord-255936-hfa9w2dg 104 3 determined determine VBD cord-255936-hfa9w2dg 104 4 the the DT cord-255936-hfa9w2dg 104 5 relative relative JJ cord-255936-hfa9w2dg 104 6 amount amount NN cord-255936-hfa9w2dg 104 7 of of IN cord-255936-hfa9w2dg 104 8 CP CP NNP cord-255936-hfa9w2dg 104 9 169e180 169e180 CD cord-255936-hfa9w2dg 104 10 epitope epitope NN cord-255936-hfa9w2dg 104 11 in in IN cord-255936-hfa9w2dg 104 12 these these DT cord-255936-hfa9w2dg 104 13 two two CD cord-255936-hfa9w2dg 104 14 vaccines vaccine NNS cord-255936-hfa9w2dg 104 15 with with IN cord-255936-hfa9w2dg 104 16 an an DT cord-255936-hfa9w2dg 104 17 enzyme enzyme NN cord-255936-hfa9w2dg 104 18 immunoassay immunoassay NN cord-255936-hfa9w2dg 104 19 employing employ VBG cord-255936-hfa9w2dg 104 20 anti anti JJ cord-255936-hfa9w2dg 104 21 - - JJ cord-255936-hfa9w2dg 104 22 CP cp JJ cord-255936-hfa9w2dg 104 23 169e180 169e180 CD cord-255936-hfa9w2dg 104 24 antibody antibody NN cord-255936-hfa9w2dg 104 25 ( ( -LRB- cord-255936-hfa9w2dg 104 26 Fig Fig NNP cord-255936-hfa9w2dg 104 27 . . . cord-255936-hfa9w2dg 104 28 3B 3B NNP cord-255936-hfa9w2dg 104 29 ) ) -RRB- cord-255936-hfa9w2dg 104 30 . . . cord-255936-hfa9w2dg 105 1 Because because IN cord-255936-hfa9w2dg 105 2 we -PRON- PRP cord-255936-hfa9w2dg 105 3 found find VBD cord-255936-hfa9w2dg 105 4 a a DT cord-255936-hfa9w2dg 105 5 higher high JJR cord-255936-hfa9w2dg 105 6 amount amount NN cord-255936-hfa9w2dg 105 7 of of IN cord-255936-hfa9w2dg 105 8 anti anti JJ cord-255936-hfa9w2dg 105 9 - - JJ cord-255936-hfa9w2dg 105 10 CP cp JJ cord-255936-hfa9w2dg 105 11 169e180 169e180 CD cord-255936-hfa9w2dg 105 12 antibody antibody NN cord-255936-hfa9w2dg 105 13 bound bind VBN cord-255936-hfa9w2dg 105 14 to to TO cord-255936-hfa9w2dg 105 15 vaccine vaccine VB cord-255936-hfa9w2dg 105 16 A A NNP cord-255936-hfa9w2dg 105 17 - - HYPH cord-255936-hfa9w2dg 105 18 coated coated JJ cord-255936-hfa9w2dg 105 19 wells well NNS cord-255936-hfa9w2dg 105 20 than than IN cord-255936-hfa9w2dg 105 21 in in IN cord-255936-hfa9w2dg 105 22 vaccine vaccine NN cord-255936-hfa9w2dg 105 23 B b NN cord-255936-hfa9w2dg 105 24 - - HYPH cord-255936-hfa9w2dg 105 25 coated coat VBN cord-255936-hfa9w2dg 105 26 wells well NNS cord-255936-hfa9w2dg 105 27 , , , cord-255936-hfa9w2dg 105 28 we -PRON- PRP cord-255936-hfa9w2dg 105 29 concluded conclude VBD cord-255936-hfa9w2dg 105 30 that that DT cord-255936-hfa9w2dg 105 31 vaccine vaccine NN cord-255936-hfa9w2dg 105 32 A a DT cord-255936-hfa9w2dg 105 33 contained contain VBD cord-255936-hfa9w2dg 105 34 a a DT cord-255936-hfa9w2dg 105 35 higher high JJR cord-255936-hfa9w2dg 105 36 amount amount NN cord-255936-hfa9w2dg 105 37 of of IN cord-255936-hfa9w2dg 105 38 CP CP NNP cord-255936-hfa9w2dg 105 39 169e180 169e180 CD cord-255936-hfa9w2dg 105 40 epitope epitope NN cord-255936-hfa9w2dg 105 41 . . . cord-255936-hfa9w2dg 106 1 To to TO cord-255936-hfa9w2dg 106 2 monitor monitor VB cord-255936-hfa9w2dg 106 3 the the DT cord-255936-hfa9w2dg 106 4 humoral humoral JJ cord-255936-hfa9w2dg 106 5 response response NN cord-255936-hfa9w2dg 106 6 , , , cord-255936-hfa9w2dg 106 7 we -PRON- PRP cord-255936-hfa9w2dg 106 8 collected collect VBD cord-255936-hfa9w2dg 106 9 sera sera NNP cord-255936-hfa9w2dg 106 10 from from IN cord-255936-hfa9w2dg 106 11 guinea guinea NN cord-255936-hfa9w2dg 106 12 pigs pig NNS cord-255936-hfa9w2dg 106 13 ( ( -LRB- cord-255936-hfa9w2dg 106 14 n n NN cord-255936-hfa9w2dg 106 15 ¼ ¼ NNP cord-255936-hfa9w2dg 106 16 5 5 CD cord-255936-hfa9w2dg 106 17 / / SYM cord-255936-hfa9w2dg 106 18 group group NN cord-255936-hfa9w2dg 106 19 ) ) -RRB- cord-255936-hfa9w2dg 106 20 that that WDT cord-255936-hfa9w2dg 106 21 were be VBD cord-255936-hfa9w2dg 106 22 immunized immunize VBN cord-255936-hfa9w2dg 106 23 with with IN cord-255936-hfa9w2dg 106 24 vaccines vaccine NNS cord-255936-hfa9w2dg 106 25 . . . cord-255936-hfa9w2dg 107 1 For for IN cord-255936-hfa9w2dg 107 2 control control NNP cord-255936-hfa9w2dg 107 3 sera sera NNP cord-255936-hfa9w2dg 107 4 , , , cord-255936-hfa9w2dg 107 5 we -PRON- PRP cord-255936-hfa9w2dg 107 6 also also RB cord-255936-hfa9w2dg 107 7 immunized immunize VBD cord-255936-hfa9w2dg 107 8 with with IN cord-255936-hfa9w2dg 107 9 VLPs vlp NNS cord-255936-hfa9w2dg 107 10 and and CC cord-255936-hfa9w2dg 107 11 the the DT cord-255936-hfa9w2dg 107 12 recombinant recombinant JJ cord-255936-hfa9w2dg 107 13 delN deln JJ cord-255936-hfa9w2dg 107 14 CP cp NN cord-255936-hfa9w2dg 107 15 in in IN cord-255936-hfa9w2dg 107 16 parallel parallel NN cord-255936-hfa9w2dg 107 17 . . . cord-255936-hfa9w2dg 108 1 To to TO cord-255936-hfa9w2dg 108 2 confirm confirm VB cord-255936-hfa9w2dg 108 3 successful successful JJ cord-255936-hfa9w2dg 108 4 vaccination vaccination NN cord-255936-hfa9w2dg 108 5 , , , cord-255936-hfa9w2dg 108 6 we -PRON- PRP cord-255936-hfa9w2dg 108 7 performed perform VBD cord-255936-hfa9w2dg 108 8 IFA IFA NNP cord-255936-hfa9w2dg 108 9 as as IN cord-255936-hfa9w2dg 108 10 described describe VBN cord-255936-hfa9w2dg 108 11 previously previously RB cord-255936-hfa9w2dg 108 12 using use VBG cord-255936-hfa9w2dg 108 13 PCV2-infected PCV2-infected NNP cord-255936-hfa9w2dg 108 14 PK-15 PK-15 NNP cord-255936-hfa9w2dg 108 15 cells cell NNS cord-255936-hfa9w2dg 108 16 [ [ -LRB- cord-255936-hfa9w2dg 108 17 18 18 CD cord-255936-hfa9w2dg 108 18 , , , cord-255936-hfa9w2dg 108 19 29 29 CD cord-255936-hfa9w2dg 108 20 ] ] -RRB- cord-255936-hfa9w2dg 108 21 . . . cord-255936-hfa9w2dg 109 1 There there EX cord-255936-hfa9w2dg 109 2 was be VBD cord-255936-hfa9w2dg 109 3 no no DT cord-255936-hfa9w2dg 109 4 statistical statistical JJ cord-255936-hfa9w2dg 109 5 difference difference NN cord-255936-hfa9w2dg 109 6 between between IN cord-255936-hfa9w2dg 109 7 the the DT cord-255936-hfa9w2dg 109 8 antibody antibody NN cord-255936-hfa9w2dg 109 9 titer titer NN cord-255936-hfa9w2dg 109 10 of of IN cord-255936-hfa9w2dg 109 11 sera sera NNP cord-255936-hfa9w2dg 109 12 obtained obtain VBN cord-255936-hfa9w2dg 109 13 from from IN cord-255936-hfa9w2dg 109 14 animals animal NNS cord-255936-hfa9w2dg 109 15 immunized immunize VBN cord-255936-hfa9w2dg 109 16 with with IN cord-255936-hfa9w2dg 109 17 the the DT cord-255936-hfa9w2dg 109 18 two two CD cord-255936-hfa9w2dg 109 19 vaccines vaccine NNS cord-255936-hfa9w2dg 109 20 . . . cord-255936-hfa9w2dg 110 1 As as IN cord-255936-hfa9w2dg 110 2 expected expect VBN cord-255936-hfa9w2dg 110 3 , , , cord-255936-hfa9w2dg 110 4 the the DT cord-255936-hfa9w2dg 110 5 sera sera NNP cord-255936-hfa9w2dg 110 6 of of IN cord-255936-hfa9w2dg 110 7 animals animal NNS cord-255936-hfa9w2dg 110 8 immunized immunize VBN cord-255936-hfa9w2dg 110 9 with with IN cord-255936-hfa9w2dg 110 10 delN deln CD cord-255936-hfa9w2dg 110 11 CP CP NNP cord-255936-hfa9w2dg 110 12 showed show VBD cord-255936-hfa9w2dg 110 13 a a DT cord-255936-hfa9w2dg 110 14 statistically statistically RB cord-255936-hfa9w2dg 110 15 lower low JJR cord-255936-hfa9w2dg 110 16 antibody antibody NN cord-255936-hfa9w2dg 110 17 titer titer NN cord-255936-hfa9w2dg 110 18 compared compare VBN cord-255936-hfa9w2dg 110 19 to to IN cord-255936-hfa9w2dg 110 20 other other JJ cord-255936-hfa9w2dg 110 21 groups group NNS cord-255936-hfa9w2dg 110 22 ( ( -LRB- cord-255936-hfa9w2dg 110 23 p p NN cord-255936-hfa9w2dg 110 24 < < XX cord-255936-hfa9w2dg 110 25 0.05 0.05 CD cord-255936-hfa9w2dg 110 26 ) ) -RRB- cord-255936-hfa9w2dg 111 1 ( ( -LRB- cord-255936-hfa9w2dg 111 2 Fig Fig NNP cord-255936-hfa9w2dg 111 3 . . . cord-255936-hfa9w2dg 111 4 3C 3C NNP cord-255936-hfa9w2dg 111 5 ) ) -RRB- cord-255936-hfa9w2dg 111 6 . . . cord-255936-hfa9w2dg 112 1 In in IN cord-255936-hfa9w2dg 112 2 an an DT cord-255936-hfa9w2dg 112 3 enzyme enzyme NN cord-255936-hfa9w2dg 112 4 immunoassay immunoassay NN cord-255936-hfa9w2dg 112 5 employing employ VBG cord-255936-hfa9w2dg 112 6 a a DT cord-255936-hfa9w2dg 112 7 microtiter microtiter NN cord-255936-hfa9w2dg 112 8 plate plate NN cord-255936-hfa9w2dg 112 9 coated coat VBN cord-255936-hfa9w2dg 112 10 with with IN cord-255936-hfa9w2dg 112 11 BSA BSA NNP cord-255936-hfa9w2dg 112 12 - - HYPH cord-255936-hfa9w2dg 112 13 conjugated conjugate VBN cord-255936-hfa9w2dg 112 14 CP CP NNP cord-255936-hfa9w2dg 112 15 169e180 169e180 CD cord-255936-hfa9w2dg 112 16 peptide peptide NN cord-255936-hfa9w2dg 112 17 , , , cord-255936-hfa9w2dg 112 18 sera sera NNP cord-255936-hfa9w2dg 112 19 from from IN cord-255936-hfa9w2dg 112 20 animals animal NNS cord-255936-hfa9w2dg 112 21 immunized immunize VBN cord-255936-hfa9w2dg 112 22 with with IN cord-255936-hfa9w2dg 113 1 vaccine vaccine NN cord-255936-hfa9w2dg 113 2 A A NNP cord-255936-hfa9w2dg 113 3 had have VBD cord-255936-hfa9w2dg 113 4 a a DT cord-255936-hfa9w2dg 113 5 significantly significantly RB cord-255936-hfa9w2dg 113 6 higher high JJR cord-255936-hfa9w2dg 113 7 antibody antibody NN cord-255936-hfa9w2dg 113 8 titer titer NN cord-255936-hfa9w2dg 113 9 than than IN cord-255936-hfa9w2dg 113 10 vaccine vaccine NN cord-255936-hfa9w2dg 113 11 B b NN cord-255936-hfa9w2dg 113 12 - - HYPH cord-255936-hfa9w2dg 113 13 vaccinated vaccinate VBN cord-255936-hfa9w2dg 113 14 animals animal NNS cord-255936-hfa9w2dg 113 15 ( ( -LRB- cord-255936-hfa9w2dg 113 16 p p NN cord-255936-hfa9w2dg 113 17 < < XX cord-255936-hfa9w2dg 113 18 0.01 0.01 CD cord-255936-hfa9w2dg 113 19 ) ) -RRB- cord-255936-hfa9w2dg 113 20 ( ( -LRB- cord-255936-hfa9w2dg 113 21 Fig Fig NNP cord-255936-hfa9w2dg 113 22 . . . cord-255936-hfa9w2dg 113 23 3D 3D NNP cord-255936-hfa9w2dg 113 24 ) ) -RRB- cord-255936-hfa9w2dg 113 25 . . . cord-255936-hfa9w2dg 114 1 Additionally additionally RB cord-255936-hfa9w2dg 114 2 , , , cord-255936-hfa9w2dg 114 3 the the DT cord-255936-hfa9w2dg 114 4 sera sera NNP cord-255936-hfa9w2dg 114 5 of of IN cord-255936-hfa9w2dg 114 6 delN delN NNP cord-255936-hfa9w2dg 114 7 CP CP NNP cord-255936-hfa9w2dg 114 8 - - HYPH cord-255936-hfa9w2dg 114 9 immunized immunize VBN cord-255936-hfa9w2dg 114 10 animals animal NNS cord-255936-hfa9w2dg 114 11 had have VBD cord-255936-hfa9w2dg 114 12 a a DT cord-255936-hfa9w2dg 114 13 significantly significantly RB cord-255936-hfa9w2dg 114 14 higher high JJR cord-255936-hfa9w2dg 114 15 antibody antibody NN cord-255936-hfa9w2dg 114 16 titer titer NN cord-255936-hfa9w2dg 114 17 than than IN cord-255936-hfa9w2dg 114 18 those those DT cord-255936-hfa9w2dg 114 19 of of IN cord-255936-hfa9w2dg 114 20 VLP VLP NNP cord-255936-hfa9w2dg 114 21 - - HYPH cord-255936-hfa9w2dg 114 22 immunized immunize VBN cord-255936-hfa9w2dg 114 23 animals animal NNS cord-255936-hfa9w2dg 115 1 ( ( -LRB- cord-255936-hfa9w2dg 115 2 p p NN cord-255936-hfa9w2dg 115 3 < < XX cord-255936-hfa9w2dg 115 4 0.01 0.01 CD cord-255936-hfa9w2dg 115 5 ) ) -RRB- cord-255936-hfa9w2dg 115 6 . . . cord-255936-hfa9w2dg 116 1 Then then RB cord-255936-hfa9w2dg 116 2 we -PRON- PRP cord-255936-hfa9w2dg 116 3 measured measure VBD cord-255936-hfa9w2dg 116 4 and and CC cord-255936-hfa9w2dg 116 5 compared compare VBD cord-255936-hfa9w2dg 116 6 the the DT cord-255936-hfa9w2dg 116 7 neutralizing neutralize VBG cord-255936-hfa9w2dg 116 8 activity activity NN cord-255936-hfa9w2dg 116 9 of of IN cord-255936-hfa9w2dg 116 10 sera sera NNP cord-255936-hfa9w2dg 116 11 in in IN cord-255936-hfa9w2dg 116 12 an an DT cord-255936-hfa9w2dg 116 13 in in FW cord-255936-hfa9w2dg 116 14 vitro vitro FW cord-255936-hfa9w2dg 116 15 infection infection NN cord-255936-hfa9w2dg 116 16 experiment experiment NN cord-255936-hfa9w2dg 116 17 employing employ VBG cord-255936-hfa9w2dg 116 18 PCV2 pcv2 JJ cord-255936-hfa9w2dg 116 19 virus virus NN cord-255936-hfa9w2dg 116 20 and and CC cord-255936-hfa9w2dg 116 21 PK-15 PK-15 NNP cord-255936-hfa9w2dg 116 22 cells cell NNS cord-255936-hfa9w2dg 116 23 . . . cord-255936-hfa9w2dg 117 1 The the DT cord-255936-hfa9w2dg 117 2 sera sera NNP cord-255936-hfa9w2dg 117 3 from from IN cord-255936-hfa9w2dg 117 4 vaccine vaccine NN cord-255936-hfa9w2dg 117 5 B B NNP cord-255936-hfa9w2dg 117 6 - - HYPH cord-255936-hfa9w2dg 117 7 immunized immunize VBN cord-255936-hfa9w2dg 117 8 animals animal NNS cord-255936-hfa9w2dg 117 9 had have VBD cord-255936-hfa9w2dg 117 10 a a DT cord-255936-hfa9w2dg 117 11 significantly significantly RB cord-255936-hfa9w2dg 117 12 higher high JJR cord-255936-hfa9w2dg 117 13 neutralizing neutralize VBG cord-255936-hfa9w2dg 117 14 antibody antibody NN cord-255936-hfa9w2dg 117 15 titer titer NN cord-255936-hfa9w2dg 117 16 than than IN cord-255936-hfa9w2dg 117 17 sera sera NNP cord-255936-hfa9w2dg 117 18 from from IN cord-255936-hfa9w2dg 117 19 vaccine vaccine NN cord-255936-hfa9w2dg 117 20 A A NNP cord-255936-hfa9w2dg 117 21 - - HYPH cord-255936-hfa9w2dg 117 22 immunized immunize VBN cord-255936-hfa9w2dg 117 23 animals animal NNS cord-255936-hfa9w2dg 117 24 ( ( -LRB- cord-255936-hfa9w2dg 117 25 p p NN cord-255936-hfa9w2dg 117 26 < < XX cord-255936-hfa9w2dg 117 27 0.001 0.001 CD cord-255936-hfa9w2dg 117 28 ) ) -RRB- cord-255936-hfa9w2dg 118 1 ( ( -LRB- cord-255936-hfa9w2dg 118 2 Fig Fig NNP cord-255936-hfa9w2dg 118 3 . . . cord-255936-hfa9w2dg 118 4 3E 3E NNP cord-255936-hfa9w2dg 118 5 ) ) -RRB- cord-255936-hfa9w2dg 118 6 . . . cord-255936-hfa9w2dg 119 1 Also also RB cord-255936-hfa9w2dg 119 2 , , , cord-255936-hfa9w2dg 119 3 the the DT cord-255936-hfa9w2dg 119 4 sera sera NNP cord-255936-hfa9w2dg 119 5 of of IN cord-255936-hfa9w2dg 119 6 VLP VLP NNP cord-255936-hfa9w2dg 119 7 - - HYPH cord-255936-hfa9w2dg 119 8 immunized immunize VBN cord-255936-hfa9w2dg 119 9 animals animal NNS cord-255936-hfa9w2dg 119 10 showed show VBD cord-255936-hfa9w2dg 119 11 significantly significantly RB cord-255936-hfa9w2dg 119 12 higher high JJR cord-255936-hfa9w2dg 119 13 neutralizing neutralize VBG cord-255936-hfa9w2dg 119 14 activity activity NN cord-255936-hfa9w2dg 119 15 than than IN cord-255936-hfa9w2dg 119 16 sera sera NNP cord-255936-hfa9w2dg 119 17 of of IN cord-255936-hfa9w2dg 119 18 VLP VLP NNP cord-255936-hfa9w2dg 119 19 - - HYPH cord-255936-hfa9w2dg 119 20 immunized immunize VBN cord-255936-hfa9w2dg 119 21 animals animal NNS cord-255936-hfa9w2dg 120 1 ( ( -LRB- cord-255936-hfa9w2dg 120 2 p p NN cord-255936-hfa9w2dg 120 3 < < XX cord-255936-hfa9w2dg 120 4 0.01 0.01 CD cord-255936-hfa9w2dg 120 5 ) ) -RRB- cord-255936-hfa9w2dg 120 6 . . . cord-255936-hfa9w2dg 121 1 Since since IN cord-255936-hfa9w2dg 121 2 the the DT cord-255936-hfa9w2dg 121 3 emergence emergence NN cord-255936-hfa9w2dg 121 4 of of IN cord-255936-hfa9w2dg 121 5 PCV2 PCV2 NNP cord-255936-hfa9w2dg 121 6 , , , cord-255936-hfa9w2dg 121 7 there there EX cord-255936-hfa9w2dg 121 8 has have VBZ cord-255936-hfa9w2dg 121 9 been be VBN cord-255936-hfa9w2dg 121 10 considerable considerable JJ cord-255936-hfa9w2dg 121 11 effort effort NN cord-255936-hfa9w2dg 121 12 to to TO cord-255936-hfa9w2dg 121 13 produce produce VB cord-255936-hfa9w2dg 121 14 an an DT cord-255936-hfa9w2dg 121 15 effective effective JJ cord-255936-hfa9w2dg 121 16 vaccine vaccine NN cord-255936-hfa9w2dg 121 17 [ [ -LRB- cord-255936-hfa9w2dg 121 18 32 32 CD cord-255936-hfa9w2dg 121 19 ] ] -RRB- cord-255936-hfa9w2dg 121 20 . . . cord-255936-hfa9w2dg 122 1 For for IN cord-255936-hfa9w2dg 122 2 some some DT cord-255936-hfa9w2dg 122 3 time time NN cord-255936-hfa9w2dg 122 4 , , , cord-255936-hfa9w2dg 122 5 the the DT cord-255936-hfa9w2dg 122 6 main main JJ cord-255936-hfa9w2dg 122 7 hurdle hurdle NN cord-255936-hfa9w2dg 122 8 was be VBD cord-255936-hfa9w2dg 122 9 efficient efficient JJ cord-255936-hfa9w2dg 122 10 overexpression overexpression NN cord-255936-hfa9w2dg 122 11 of of IN cord-255936-hfa9w2dg 122 12 CP CP NNP cord-255936-hfa9w2dg 122 13 [ [ -LRB- cord-255936-hfa9w2dg 122 14 19 19 CD cord-255936-hfa9w2dg 122 15 , , , cord-255936-hfa9w2dg 122 16 33 33 CD cord-255936-hfa9w2dg 122 17 ] ] -RRB- cord-255936-hfa9w2dg 122 18 . . . cord-255936-hfa9w2dg 123 1 Later later RB cord-255936-hfa9w2dg 123 2 , , , cord-255936-hfa9w2dg 123 3 it -PRON- PRP cord-255936-hfa9w2dg 123 4 was be VBD cord-255936-hfa9w2dg 123 5 found find VBN cord-255936-hfa9w2dg 123 6 that that IN cord-255936-hfa9w2dg 123 7 the the DT cord-255936-hfa9w2dg 123 8 immunodominant immunodominant JJ cord-255936-hfa9w2dg 123 9 CP CP NNP cord-255936-hfa9w2dg 123 10 169e180 169e180 CD cord-255936-hfa9w2dg 123 11 epitope epitope NN cord-255936-hfa9w2dg 123 12 of of IN cord-255936-hfa9w2dg 123 13 PCV2 PCV2 NNP cord-255936-hfa9w2dg 123 14 is be VBZ cord-255936-hfa9w2dg 123 15 buried bury VBN cord-255936-hfa9w2dg 123 16 during during IN cord-255936-hfa9w2dg 123 17 viral viral JJ cord-255936-hfa9w2dg 123 18 capsid capsid NN cord-255936-hfa9w2dg 123 19 assembly assembly NN cord-255936-hfa9w2dg 123 20 and and CC cord-255936-hfa9w2dg 123 21 that that IN cord-255936-hfa9w2dg 123 22 the the DT cord-255936-hfa9w2dg 123 23 antibodies antibody NNS cord-255936-hfa9w2dg 123 24 targeting target VBG cord-255936-hfa9w2dg 123 25 this this DT cord-255936-hfa9w2dg 123 26 epitope epitope NN cord-255936-hfa9w2dg 123 27 are be VBP cord-255936-hfa9w2dg 123 28 non non JJ cord-255936-hfa9w2dg 123 29 - - JJ cord-255936-hfa9w2dg 123 30 neutralizing neutralizing JJ cord-255936-hfa9w2dg 123 31 , , , cord-255936-hfa9w2dg 123 32 which which WDT cord-255936-hfa9w2dg 123 33 is be VBZ cord-255936-hfa9w2dg 123 34 a a DT cord-255936-hfa9w2dg 123 35 common common JJ cord-255936-hfa9w2dg 123 36 viral viral JJ cord-255936-hfa9w2dg 123 37 immune immune JJ cord-255936-hfa9w2dg 123 38 - - HYPH cord-255936-hfa9w2dg 123 39 evasion evasion NN cord-255936-hfa9w2dg 123 40 mechanism mechanism NN cord-255936-hfa9w2dg 123 41 [ [ -LRB- cord-255936-hfa9w2dg 123 42 14 14 CD cord-255936-hfa9w2dg 123 43 ] ] -RRB- cord-255936-hfa9w2dg 123 44 . . . cord-255936-hfa9w2dg 124 1 PCV2 pcv2 JJ cord-255936-hfa9w2dg 124 2 infection infection NN cord-255936-hfa9w2dg 124 3 inevitably inevitably RB cord-255936-hfa9w2dg 124 4 provokes provoke VBZ cord-255936-hfa9w2dg 124 5 exposure exposure NN cord-255936-hfa9w2dg 124 6 to to IN cord-255936-hfa9w2dg 124 7 the the DT cord-255936-hfa9w2dg 124 8 decoy decoy NN cord-255936-hfa9w2dg 124 9 epitope epitope NN cord-255936-hfa9w2dg 124 10 and and CC cord-255936-hfa9w2dg 124 11 therefore therefore RB cord-255936-hfa9w2dg 124 12 is be VBZ cord-255936-hfa9w2dg 124 13 less less RBR cord-255936-hfa9w2dg 124 14 effective effective JJ cord-255936-hfa9w2dg 124 15 at at IN cord-255936-hfa9w2dg 124 16 generating generate VBG cord-255936-hfa9w2dg 124 17 a a DT cord-255936-hfa9w2dg 124 18 protective protective JJ cord-255936-hfa9w2dg 124 19 humoral humoral JJ cord-255936-hfa9w2dg 124 20 response response NN cord-255936-hfa9w2dg 124 21 than than IN cord-255936-hfa9w2dg 124 22 immunization immunization NN cord-255936-hfa9w2dg 124 23 of of IN cord-255936-hfa9w2dg 124 24 the the DT cord-255936-hfa9w2dg 124 25 subunit subunit NN cord-255936-hfa9w2dg 124 26 vaccine vaccine NN cord-255936-hfa9w2dg 124 27 with with IN cord-255936-hfa9w2dg 124 28 a a DT cord-255936-hfa9w2dg 124 29 minimal minimal JJ cord-255936-hfa9w2dg 124 30 amount amount NN cord-255936-hfa9w2dg 124 31 of of IN cord-255936-hfa9w2dg 124 32 exposed expose VBN cord-255936-hfa9w2dg 124 33 decoy decoy NN cord-255936-hfa9w2dg 124 34 epitope epitope NN cord-255936-hfa9w2dg 124 35 [ [ -LRB- cord-255936-hfa9w2dg 124 36 18 18 CD cord-255936-hfa9w2dg 124 37 ] ] -RRB- cord-255936-hfa9w2dg 124 38 . . . cord-255936-hfa9w2dg 125 1 Researchers researcher NNS cord-255936-hfa9w2dg 125 2 had have VBD cord-255936-hfa9w2dg 125 3 predicted predict VBN cord-255936-hfa9w2dg 125 4 that that IN cord-255936-hfa9w2dg 125 5 incomplete incomplete JJ cord-255936-hfa9w2dg 125 6 VLPs vlp NNS cord-255936-hfa9w2dg 125 7 with with IN cord-255936-hfa9w2dg 125 8 an an DT cord-255936-hfa9w2dg 125 9 exposed expose VBN cord-255936-hfa9w2dg 125 10 immunodominant immunodominant NN cord-255936-hfa9w2dg 125 11 CP CP NNP cord-255936-hfa9w2dg 125 12 169e180 169e180 CD cord-255936-hfa9w2dg 125 13 epitope epitope NN cord-255936-hfa9w2dg 125 14 can can MD cord-255936-hfa9w2dg 125 15 be be VB cord-255936-hfa9w2dg 125 16 contaminated contaminate VBN cord-255936-hfa9w2dg 125 17 during during IN cord-255936-hfa9w2dg 125 18 the the DT cord-255936-hfa9w2dg 125 19 preparation preparation NN cord-255936-hfa9w2dg 125 20 of of IN cord-255936-hfa9w2dg 125 21 subunit subunit NN cord-255936-hfa9w2dg 125 22 vaccine vaccine NN cord-255936-hfa9w2dg 125 23 and and CC cord-255936-hfa9w2dg 125 24 drive drive VB cord-255936-hfa9w2dg 125 25 the the DT cord-255936-hfa9w2dg 125 26 humoral humoral JJ cord-255936-hfa9w2dg 125 27 response response NN cord-255936-hfa9w2dg 125 28 to to TO cord-255936-hfa9w2dg 125 29 produce produce VB cord-255936-hfa9w2dg 125 30 non non JJ cord-255936-hfa9w2dg 125 31 - - JJ cord-255936-hfa9w2dg 125 32 neutralizing neutralize VBG cord-255936-hfa9w2dg 125 33 antibodies antibody NNS cord-255936-hfa9w2dg 125 34 , , , cord-255936-hfa9w2dg 125 35 but but CC cord-255936-hfa9w2dg 125 36 could could MD cord-255936-hfa9w2dg 125 37 not not RB cord-255936-hfa9w2dg 125 38 quantify quantify VB cord-255936-hfa9w2dg 125 39 the the DT cord-255936-hfa9w2dg 125 40 exposed expose VBN cord-255936-hfa9w2dg 125 41 immunodominant immunodominant JJ cord-255936-hfa9w2dg 125 42 CP CP NNP cord-255936-hfa9w2dg 125 43 169e180 169e180 CD cord-255936-hfa9w2dg 125 44 epitope epitope NN cord-255936-hfa9w2dg 125 45 in in IN cord-255936-hfa9w2dg 125 46 the the DT cord-255936-hfa9w2dg 125 47 subunit subunit NN cord-255936-hfa9w2dg 125 48 vaccines vaccine NNS cord-255936-hfa9w2dg 125 49 because because IN cord-255936-hfa9w2dg 125 50 of of IN cord-255936-hfa9w2dg 125 51 the the DT cord-255936-hfa9w2dg 125 52 lack lack NN cord-255936-hfa9w2dg 125 53 of of IN cord-255936-hfa9w2dg 125 54 proper proper JJ cord-255936-hfa9w2dg 125 55 analytical analytical JJ cord-255936-hfa9w2dg 125 56 tools tool NNS cord-255936-hfa9w2dg 125 57 [ [ -LRB- cord-255936-hfa9w2dg 125 58 33e36 33e36 CD cord-255936-hfa9w2dg 125 59 ] ] -RRB- cord-255936-hfa9w2dg 125 60 . . . cord-255936-hfa9w2dg 126 1 To to TO cord-255936-hfa9w2dg 126 2 rectify rectify VB cord-255936-hfa9w2dg 126 3 this this DT cord-255936-hfa9w2dg 126 4 , , , cord-255936-hfa9w2dg 126 5 we -PRON- PRP cord-255936-hfa9w2dg 126 6 generated generate VBD cord-255936-hfa9w2dg 126 7 a a DT cord-255936-hfa9w2dg 126 8 monoclonal monoclonal JJ cord-255936-hfa9w2dg 126 9 antibody antibody NN cord-255936-hfa9w2dg 126 10 specific specific JJ cord-255936-hfa9w2dg 126 11 to to IN cord-255936-hfa9w2dg 126 12 the the DT cord-255936-hfa9w2dg 126 13 immunodominant immunodominant JJ cord-255936-hfa9w2dg 126 14 CP CP NNP cord-255936-hfa9w2dg 126 15 169e180 169e180 CD cord-255936-hfa9w2dg 126 16 epitope epitope NN cord-255936-hfa9w2dg 126 17 from from IN cord-255936-hfa9w2dg 126 18 an an DT cord-255936-hfa9w2dg 126 19 antibody antibody NN cord-255936-hfa9w2dg 126 20 library library NN cord-255936-hfa9w2dg 126 21 generated generate VBN cord-255936-hfa9w2dg 126 22 using use VBG cord-255936-hfa9w2dg 126 23 chickens chicken NNS cord-255936-hfa9w2dg 126 24 immunized immunize VBN cord-255936-hfa9w2dg 126 25 with with IN cord-255936-hfa9w2dg 126 26 CP CP NNP cord-255936-hfa9w2dg 126 27 169e180 169e180 CD cord-255936-hfa9w2dg 126 28 peptide peptide NN cord-255936-hfa9w2dg 126 29 . . . cord-255936-hfa9w2dg 127 1 This this DT cord-255936-hfa9w2dg 127 2 recombinant recombinant JJ cord-255936-hfa9w2dg 127 3 anti anti JJ cord-255936-hfa9w2dg 127 4 - - JJ cord-255936-hfa9w2dg 127 5 CP cp JJ cord-255936-hfa9w2dg 127 6 169e180 169e180 CD cord-255936-hfa9w2dg 127 7 antibody antibody NN cord-255936-hfa9w2dg 127 8 preferentially preferentially RB cord-255936-hfa9w2dg 127 9 reacted react VBD cord-255936-hfa9w2dg 127 10 to to IN cord-255936-hfa9w2dg 127 11 delN deln JJ cord-255936-hfa9w2dg 127 12 CP CP NNP cord-255936-hfa9w2dg 127 13 with with IN cord-255936-hfa9w2dg 127 14 an an DT cord-255936-hfa9w2dg 127 15 exposed expose VBN cord-255936-hfa9w2dg 127 16 CP CP NNP cord-255936-hfa9w2dg 127 17 169e180 169e180 CD cord-255936-hfa9w2dg 127 18 epitope epitope NN cord-255936-hfa9w2dg 127 19 compared compare VBN cord-255936-hfa9w2dg 127 20 to to IN cord-255936-hfa9w2dg 127 21 VLP VLP NNP cord-255936-hfa9w2dg 127 22 in in IN cord-255936-hfa9w2dg 127 23 an an DT cord-255936-hfa9w2dg 127 24 enzyme enzyme NN cord-255936-hfa9w2dg 127 25 immunoassay immunoassay NN cord-255936-hfa9w2dg 127 26 ( ( -LRB- cord-255936-hfa9w2dg 127 27 Fig Fig NNP cord-255936-hfa9w2dg 127 28 . . . cord-255936-hfa9w2dg 127 29 1B 1b CD cord-255936-hfa9w2dg 127 30 ) ) -RRB- cord-255936-hfa9w2dg 127 31 . . . cord-255936-hfa9w2dg 128 1 Because because IN cord-255936-hfa9w2dg 128 2 the the DT cord-255936-hfa9w2dg 128 3 length length NN cord-255936-hfa9w2dg 128 4 of of IN cord-255936-hfa9w2dg 128 5 linear linear JJ cord-255936-hfa9w2dg 128 6 B b NN cord-255936-hfa9w2dg 128 7 - - HYPH cord-255936-hfa9w2dg 128 8 cell cell NN cord-255936-hfa9w2dg 128 9 epitopes epitope NNS cord-255936-hfa9w2dg 128 10 can can MD cord-255936-hfa9w2dg 128 11 vary vary VB cord-255936-hfa9w2dg 128 12 widely widely RB cord-255936-hfa9w2dg 128 13 from from IN cord-255936-hfa9w2dg 128 14 3 3 CD cord-255936-hfa9w2dg 128 15 to to TO cord-255936-hfa9w2dg 128 16 38 38 CD cord-255936-hfa9w2dg 128 17 amino amino NN cord-255936-hfa9w2dg 128 18 acids acid NNS cord-255936-hfa9w2dg 128 19 [ [ -LRB- cord-255936-hfa9w2dg 128 20 37 37 CD cord-255936-hfa9w2dg 128 21 ] ] -RRB- cord-255936-hfa9w2dg 128 22 , , , cord-255936-hfa9w2dg 128 23 we -PRON- PRP cord-255936-hfa9w2dg 128 24 confirmed confirm VBD cord-255936-hfa9w2dg 128 25 that that IN cord-255936-hfa9w2dg 128 26 CP CP NNP cord-255936-hfa9w2dg 128 27 169e180 169e180 CD cord-255936-hfa9w2dg 128 28 peptides peptide NNS cord-255936-hfa9w2dg 128 29 behave behave VBP cord-255936-hfa9w2dg 128 30 as as IN cord-255936-hfa9w2dg 128 31 a a DT cord-255936-hfa9w2dg 128 32 single single JJ cord-255936-hfa9w2dg 128 33 epitope epitope NN cord-255936-hfa9w2dg 128 34 in in IN cord-255936-hfa9w2dg 128 35 a a DT cord-255936-hfa9w2dg 128 36 competition competition NN cord-255936-hfa9w2dg 128 37 enzyme enzyme NN cord-255936-hfa9w2dg 128 38 immunoassay immunoassay NN cord-255936-hfa9w2dg 128 39 . . . cord-255936-hfa9w2dg 129 1 The the DT cord-255936-hfa9w2dg 129 2 recombinant recombinant JJ cord-255936-hfa9w2dg 129 3 CP CP NNP cord-255936-hfa9w2dg 129 4 169e180 169e180 CD cord-255936-hfa9w2dg 129 5 antibody antibody NN cord-255936-hfa9w2dg 129 6 almost almost RB cord-255936-hfa9w2dg 129 7 completely completely RB cord-255936-hfa9w2dg 129 8 inhibited inhibit VBD cord-255936-hfa9w2dg 129 9 the the DT cord-255936-hfa9w2dg 129 10 binding binding NN cord-255936-hfa9w2dg 129 11 of of IN cord-255936-hfa9w2dg 129 12 PCV2-infected pcv2-infected JJ cord-255936-hfa9w2dg 129 13 pig pig NN cord-255936-hfa9w2dg 129 14 sera sera NNP cord-255936-hfa9w2dg 129 15 to to IN cord-255936-hfa9w2dg 129 16 CP CP NNP cord-255936-hfa9w2dg 129 17 169e180 169e180 CD cord-255936-hfa9w2dg 129 18 peptide peptide NN cord-255936-hfa9w2dg 129 19 . . . cord-255936-hfa9w2dg 130 1 We -PRON- PRP cord-255936-hfa9w2dg 130 2 then then RB cord-255936-hfa9w2dg 130 3 used use VBD cord-255936-hfa9w2dg 130 4 this this DT cord-255936-hfa9w2dg 130 5 antibody antibody NN cord-255936-hfa9w2dg 130 6 to to TO cord-255936-hfa9w2dg 130 7 quantify quantify VB cord-255936-hfa9w2dg 130 8 the the DT cord-255936-hfa9w2dg 130 9 relative relative JJ cord-255936-hfa9w2dg 130 10 amount amount NN cord-255936-hfa9w2dg 130 11 of of IN cord-255936-hfa9w2dg 130 12 CP CP NNP cord-255936-hfa9w2dg 130 13 169e180 169e180 CD cord-255936-hfa9w2dg 130 14 epitope epitope NN cord-255936-hfa9w2dg 130 15 in in IN cord-255936-hfa9w2dg 130 16 two two CD cord-255936-hfa9w2dg 130 17 commercially commercially RB cord-255936-hfa9w2dg 130 18 available available JJ cord-255936-hfa9w2dg 130 19 vaccines vaccine NNS cord-255936-hfa9w2dg 130 20 and and CC cord-255936-hfa9w2dg 130 21 found find VBD cord-255936-hfa9w2dg 130 22 that that DT cord-255936-hfa9w2dg 130 23 vaccine vaccine NN cord-255936-hfa9w2dg 130 24 A a DT cord-255936-hfa9w2dg 130 25 contained contain VBD cord-255936-hfa9w2dg 130 26 significantly significantly RB cord-255936-hfa9w2dg 130 27 more more JJR cord-255936-hfa9w2dg 130 28 decoy decoy NN cord-255936-hfa9w2dg 130 29 epitope epitope NN cord-255936-hfa9w2dg 130 30 than than IN cord-255936-hfa9w2dg 130 31 vaccine vaccine NN cord-255936-hfa9w2dg 131 1 B. B. NNP cord-255936-hfa9w2dg 132 1 After after IN cord-255936-hfa9w2dg 132 2 immunization immunization NN cord-255936-hfa9w2dg 132 3 , , , cord-255936-hfa9w2dg 132 4 vaccine vaccine NN cord-255936-hfa9w2dg 132 5 A a DT cord-255936-hfa9w2dg 132 6 induced induce VBN cord-255936-hfa9w2dg 132 7 a a DT cord-255936-hfa9w2dg 132 8 significantly significantly RB cord-255936-hfa9w2dg 132 9 higher high JJR cord-255936-hfa9w2dg 132 10 antibody antibody NN cord-255936-hfa9w2dg 132 11 response response NN cord-255936-hfa9w2dg 132 12 to to IN cord-255936-hfa9w2dg 132 13 decoy decoy NN cord-255936-hfa9w2dg 132 14 epitope epitope NN cord-255936-hfa9w2dg 132 15 than than IN cord-255936-hfa9w2dg 132 16 vaccine vaccine NN cord-255936-hfa9w2dg 132 17 B b NN cord-255936-hfa9w2dg 132 18 , , , cord-255936-hfa9w2dg 132 19 which which WDT cord-255936-hfa9w2dg 132 20 supported support VBD cord-255936-hfa9w2dg 132 21 the the DT cord-255936-hfa9w2dg 132 22 finding finding NN cord-255936-hfa9w2dg 132 23 that that DT cord-255936-hfa9w2dg 132 24 vaccine vaccine NN cord-255936-hfa9w2dg 132 25 B b NN cord-255936-hfa9w2dg 132 26 induced induce VBD cord-255936-hfa9w2dg 132 27 a a DT cord-255936-hfa9w2dg 132 28 higher high JJR cord-255936-hfa9w2dg 132 29 amount amount NN cord-255936-hfa9w2dg 132 30 of of IN cord-255936-hfa9w2dg 132 31 neutralizing neutralize VBG cord-255936-hfa9w2dg 132 32 antibody antibody NN cord-255936-hfa9w2dg 132 33 . . . cord-255936-hfa9w2dg 133 1 In in IN cord-255936-hfa9w2dg 133 2 summary summary NN cord-255936-hfa9w2dg 133 3 , , , cord-255936-hfa9w2dg 133 4 our -PRON- PRP$ cord-255936-hfa9w2dg 133 5 research research NN cord-255936-hfa9w2dg 133 6 demonstrated demonstrate VBD cord-255936-hfa9w2dg 133 7 that that IN cord-255936-hfa9w2dg 133 8 the the DT cord-255936-hfa9w2dg 133 9 amount amount NN cord-255936-hfa9w2dg 133 10 of of IN cord-255936-hfa9w2dg 133 11 immunodominant immunodominant JJ cord-255936-hfa9w2dg 133 12 decoy decoy NN cord-255936-hfa9w2dg 133 13 epitope epitope NN cord-255936-hfa9w2dg 133 14 present present JJ cord-255936-hfa9w2dg 133 15 in in IN cord-255936-hfa9w2dg 133 16 PCV2 pcv2 JJ cord-255936-hfa9w2dg 133 17 vaccine vaccine NN cord-255936-hfa9w2dg 133 18 can can MD cord-255936-hfa9w2dg 133 19 be be VB cord-255936-hfa9w2dg 133 20 measured measure VBN cord-255936-hfa9w2dg 133 21 by by IN cord-255936-hfa9w2dg 133 22 an an DT cord-255936-hfa9w2dg 133 23 epitope epitope NN cord-255936-hfa9w2dg 133 24 - - HYPH cord-255936-hfa9w2dg 133 25 specific specific JJ cord-255936-hfa9w2dg 133 26 antibody antibody NN cord-255936-hfa9w2dg 133 27 . . . cord-255936-hfa9w2dg 134 1 From from IN cord-255936-hfa9w2dg 134 2 the the DT cord-255936-hfa9w2dg 134 3 measured measured JJ cord-255936-hfa9w2dg 134 4 level level NN cord-255936-hfa9w2dg 134 5 of of IN cord-255936-hfa9w2dg 134 6 decoy decoy NN cord-255936-hfa9w2dg 134 7 epitope epitope NN cord-255936-hfa9w2dg 134 8 , , , cord-255936-hfa9w2dg 134 9 we -PRON- PRP cord-255936-hfa9w2dg 134 10 could could MD cord-255936-hfa9w2dg 134 11 predict predict VB cord-255936-hfa9w2dg 134 12 the the DT cord-255936-hfa9w2dg 134 13 efficacy efficacy NN cord-255936-hfa9w2dg 134 14 of of IN cord-255936-hfa9w2dg 134 15 a a DT cord-255936-hfa9w2dg 134 16 vaccine vaccine NN cord-255936-hfa9w2dg 134 17 for for IN cord-255936-hfa9w2dg 134 18 the the DT cord-255936-hfa9w2dg 134 19 induction induction NN cord-255936-hfa9w2dg 134 20 of of IN cord-255936-hfa9w2dg 134 21 neutralizing neutralize VBG cord-255936-hfa9w2dg 134 22 antibody antibody NN cord-255936-hfa9w2dg 134 23 titer titer NN cord-255936-hfa9w2dg 134 24 . . . cord-255936-hfa9w2dg 135 1 We -PRON- PRP cord-255936-hfa9w2dg 135 2 believe believe VBP cord-255936-hfa9w2dg 135 3 that that IN cord-255936-hfa9w2dg 135 4 anti anti JJ cord-255936-hfa9w2dg 135 5 - - JJ cord-255936-hfa9w2dg 135 6 CP cp JJ cord-255936-hfa9w2dg 135 7 169e180 169e180 CD cord-255936-hfa9w2dg 135 8 antibody antibody NN cord-255936-hfa9w2dg 135 9 can can MD cord-255936-hfa9w2dg 135 10 be be VB cord-255936-hfa9w2dg 135 11 used use VBN cord-255936-hfa9w2dg 135 12 to to TO cord-255936-hfa9w2dg 135 13 monitor monitor VB cord-255936-hfa9w2dg 135 14 the the DT cord-255936-hfa9w2dg 135 15 varying vary VBG cord-255936-hfa9w2dg 135 16 quality quality NN cord-255936-hfa9w2dg 135 17 of of IN cord-255936-hfa9w2dg 135 18 the the DT cord-255936-hfa9w2dg 135 19 subunit subunit NN cord-255936-hfa9w2dg 135 20 vaccine vaccine NN cord-255936-hfa9w2dg 135 21 from from IN cord-255936-hfa9w2dg 135 22 batch batch NN cord-255936-hfa9w2dg 135 23 to to TO cord-255936-hfa9w2dg 135 24 batch batch VB cord-255936-hfa9w2dg 135 25 . . . cord-255936-hfa9w2dg 136 1 Fig Fig NNP cord-255936-hfa9w2dg 136 2 . . NNP cord-255936-hfa9w2dg 136 3 2 2 CD cord-255936-hfa9w2dg 136 4 . . . cord-255936-hfa9w2dg 137 1 Recombinant recombinant JJ cord-255936-hfa9w2dg 137 2 anti anti JJ cord-255936-hfa9w2dg 137 3 - - JJ cord-255936-hfa9w2dg 137 4 CP cp JJ cord-255936-hfa9w2dg 137 5 169e180 169e180 CD cord-255936-hfa9w2dg 137 6 antibody antibody NN cord-255936-hfa9w2dg 137 7 inhibited inhibit VBD cord-255936-hfa9w2dg 137 8 the the DT cord-255936-hfa9w2dg 137 9 binding binding NN cord-255936-hfa9w2dg 137 10 of of IN cord-255936-hfa9w2dg 137 11 naturally naturally RB cord-255936-hfa9w2dg 137 12 occurring occur VBG cord-255936-hfa9w2dg 137 13 antibody antibody NN cord-255936-hfa9w2dg 137 14 . . . cord-255936-hfa9w2dg 138 1 After after IN cord-255936-hfa9w2dg 138 2 pre pre JJ cord-255936-hfa9w2dg 138 3 - - NN cord-255936-hfa9w2dg 138 4 incubation incubation JJ cord-255936-hfa9w2dg 138 5 with with IN cord-255936-hfa9w2dg 138 6 sera sera NNP cord-255936-hfa9w2dg 138 7 from from IN cord-255936-hfa9w2dg 138 8 the the DT cord-255936-hfa9w2dg 138 9 non non JJ cord-255936-hfa9w2dg 138 10 - - JJ cord-255936-hfa9w2dg 138 11 infected infected JJ cord-255936-hfa9w2dg 138 12 ( ( -LRB- cord-255936-hfa9w2dg 138 13 A a NN cord-255936-hfa9w2dg 138 14 ) ) -RRB- cord-255936-hfa9w2dg 138 15 and and CC cord-255936-hfa9w2dg 138 16 infected infect VBN cord-255936-hfa9w2dg 138 17 pigs pig NNS cord-255936-hfa9w2dg 138 18 ( ( -LRB- cord-255936-hfa9w2dg 138 19 B b NN cord-255936-hfa9w2dg 138 20 ) ) -RRB- cord-255936-hfa9w2dg 138 21 with with IN cord-255936-hfa9w2dg 138 22 anti anti JJ cord-255936-hfa9w2dg 138 23 - - JJ cord-255936-hfa9w2dg 138 24 CP cp JJ cord-255936-hfa9w2dg 138 25 169e180 169e180 CD cord-255936-hfa9w2dg 138 26 antibody antibody NN cord-255936-hfa9w2dg 138 27 ( ( -LRB- cord-255936-hfa9w2dg 138 28 n n NN cord-255936-hfa9w2dg 138 29 ¼ ¼ NNP cord-255936-hfa9w2dg 138 30 3 3 CD cord-255936-hfa9w2dg 138 31 / / SYM cord-255936-hfa9w2dg 138 32 group group NN cord-255936-hfa9w2dg 138 33 ) ) -RRB- cord-255936-hfa9w2dg 138 34 , , , cord-255936-hfa9w2dg 138 35 the the DT cord-255936-hfa9w2dg 138 36 mixtures mixture NNS cord-255936-hfa9w2dg 138 37 were be VBD cord-255936-hfa9w2dg 138 38 added add VBN cord-255936-hfa9w2dg 138 39 to to IN cord-255936-hfa9w2dg 138 40 a a DT cord-255936-hfa9w2dg 138 41 CP CP NNP cord-255936-hfa9w2dg 138 42 169e180 169e180 CD cord-255936-hfa9w2dg 138 43 peptide peptide NN cord-255936-hfa9w2dg 138 44 - - HYPH cord-255936-hfa9w2dg 138 45 coated coated JJ cord-255936-hfa9w2dg 138 46 microtiter microtiter NN cord-255936-hfa9w2dg 138 47 plate plate NN cord-255936-hfa9w2dg 138 48 . . . cord-255936-hfa9w2dg 139 1 The the DT cord-255936-hfa9w2dg 139 2 amount amount NN cord-255936-hfa9w2dg 139 3 of of IN cord-255936-hfa9w2dg 139 4 bound bind VBN cord-255936-hfa9w2dg 139 5 porcine porcine JJ cord-255936-hfa9w2dg 139 6 antibody antibody NN cord-255936-hfa9w2dg 139 7 was be VBD cord-255936-hfa9w2dg 139 8 measured measure VBN cord-255936-hfa9w2dg 139 9 by by IN cord-255936-hfa9w2dg 139 10 HRP HRP NNP cord-255936-hfa9w2dg 139 11 - - HYPH cord-255936-hfa9w2dg 139 12 conjugated conjugate VBN cord-255936-hfa9w2dg 139 13 anti anti JJ cord-255936-hfa9w2dg 139 14 - - JJ cord-255936-hfa9w2dg 139 15 porcine porcine JJ cord-255936-hfa9w2dg 139 16 IgG igg NN cord-255936-hfa9w2dg 139 17 antibody antibody NN cord-255936-hfa9w2dg 139 18 . . . cord-255936-hfa9w2dg 140 1 Absorbance Absorbance NNP cord-255936-hfa9w2dg 140 2 was be VBD cord-255936-hfa9w2dg 140 3 measured measure VBN cord-255936-hfa9w2dg 140 4 at at IN cord-255936-hfa9w2dg 140 5 650 650 CD cord-255936-hfa9w2dg 140 6 nm nm NN cord-255936-hfa9w2dg 140 7 . . . cord-255936-hfa9w2dg 141 1 The the DT cord-255936-hfa9w2dg 141 2 results result NNS cord-255936-hfa9w2dg 141 3 are be VBP cord-255936-hfa9w2dg 141 4 the the DT cord-255936-hfa9w2dg 141 5 means mean NNS cord-255936-hfa9w2dg 141 6 ± ± NNP cord-255936-hfa9w2dg 141 7 standard standard JJ cord-255936-hfa9w2dg 141 8 deviations deviation NNS cord-255936-hfa9w2dg 141 9 from from IN cord-255936-hfa9w2dg 141 10 the the DT cord-255936-hfa9w2dg 141 11 experiments experiment NNS cord-255936-hfa9w2dg 141 12 , , , cord-255936-hfa9w2dg 141 13 which which WDT cord-255936-hfa9w2dg 141 14 were be VBD cord-255936-hfa9w2dg 141 15 conducted conduct VBN cord-255936-hfa9w2dg 141 16 in in IN cord-255936-hfa9w2dg 141 17 triplicate triplicate NN cord-255936-hfa9w2dg 141 18 using use VBG cord-255936-hfa9w2dg 141 19 the the DT cord-255936-hfa9w2dg 141 20 sera sera NNP cord-255936-hfa9w2dg 141 21 from from IN cord-255936-hfa9w2dg 141 22 three three CD cord-255936-hfa9w2dg 141 23 pigs pig NNS cord-255936-hfa9w2dg 141 24 ( ( -LRB- cord-255936-hfa9w2dg 141 25 P1 P1 NNP cord-255936-hfa9w2dg 141 26 , , , cord-255936-hfa9w2dg 141 27 P2 P2 NNP cord-255936-hfa9w2dg 141 28 , , , cord-255936-hfa9w2dg 141 29 and and CC cord-255936-hfa9w2dg 141 30 P3 P3 NNP cord-255936-hfa9w2dg 141 31 in in IN cord-255936-hfa9w2dg 141 32 each each DT cord-255936-hfa9w2dg 141 33 group group NN cord-255936-hfa9w2dg 141 34 ) ) -RRB- cord-255936-hfa9w2dg 141 35 . . . cord-255936-hfa9w2dg 142 1 The the DT cord-255936-hfa9w2dg 142 2 authors author NNS cord-255936-hfa9w2dg 142 3 have have VBP cord-255936-hfa9w2dg 142 4 declared declare VBN cord-255936-hfa9w2dg 142 5 that that IN cord-255936-hfa9w2dg 142 6 there there EX cord-255936-hfa9w2dg 142 7 is be VBZ cord-255936-hfa9w2dg 142 8 no no DT cord-255936-hfa9w2dg 142 9 conflict conflict NN cord-255936-hfa9w2dg 142 10 of of IN cord-255936-hfa9w2dg 142 11 interest interest NN cord-255936-hfa9w2dg 142 12 . . . cord-255936-hfa9w2dg 143 1 Anti anti JJ cord-255936-hfa9w2dg 143 2 - - JJ cord-255936-hfa9w2dg 143 3 immunology immunology NN cord-255936-hfa9w2dg 143 4 : : : cord-255936-hfa9w2dg 143 5 evasion evasion NN cord-255936-hfa9w2dg 143 6 of of IN cord-255936-hfa9w2dg 143 7 the the DT cord-255936-hfa9w2dg 143 8 host host NN cord-255936-hfa9w2dg 143 9 immune immune JJ cord-255936-hfa9w2dg 143 10 system system NN cord-255936-hfa9w2dg 143 11 by by IN cord-255936-hfa9w2dg 143 12 bacterial bacterial JJ cord-255936-hfa9w2dg 143 13 and and CC cord-255936-hfa9w2dg 143 14 viral viral JJ cord-255936-hfa9w2dg 143 15 pathogens pathogen NNS cord-255936-hfa9w2dg 144 1 Neutralizing neutralize VBG cord-255936-hfa9w2dg 144 2 antibodies antibody NNS cord-255936-hfa9w2dg 144 3 generated generate VBN cord-255936-hfa9w2dg 144 4 during during IN cord-255936-hfa9w2dg 144 5 natural natural JJ cord-255936-hfa9w2dg 144 6 HIV-1 HIV-1 NNP cord-255936-hfa9w2dg 144 7 infection infection NN cord-255936-hfa9w2dg 144 8 : : : cord-255936-hfa9w2dg 144 9 good good JJ cord-255936-hfa9w2dg 144 10 news news NN cord-255936-hfa9w2dg 144 11 for for IN cord-255936-hfa9w2dg 144 12 an an DT cord-255936-hfa9w2dg 144 13 HIV-1 HIV-1 NNP cord-255936-hfa9w2dg 144 14 vaccine vaccine NN cord-255936-hfa9w2dg 144 15 ? ? . cord-255936-hfa9w2dg 145 1 Feline feline JJ cord-255936-hfa9w2dg 145 2 immunodeficiency immunodeficiency NN cord-255936-hfa9w2dg 145 3 virus virus NN cord-255936-hfa9w2dg 145 4 ( ( -LRB- cord-255936-hfa9w2dg 145 5 FIV fiv NN cord-255936-hfa9w2dg 145 6 ) ) -RRB- cord-255936-hfa9w2dg 145 7 neutralization neutralization NN cord-255936-hfa9w2dg 145 8 : : : cord-255936-hfa9w2dg 146 1 a a DT cord-255936-hfa9w2dg 146 2 review review NN cord-255936-hfa9w2dg 146 3 Immune immune JJ cord-255936-hfa9w2dg 146 4 control control NN cord-255936-hfa9w2dg 146 5 and and CC cord-255936-hfa9w2dg 146 6 failure failure NN cord-255936-hfa9w2dg 146 7 in in IN cord-255936-hfa9w2dg 146 8 HCV HCV NNP cord-255936-hfa9w2dg 146 9 infectionetipping infectionetippe VBG cord-255936-hfa9w2dg 146 10 the the DT cord-255936-hfa9w2dg 146 11 balance balance NN cord-255936-hfa9w2dg 146 12 Xenoepitope Xenoepitope NNP cord-255936-hfa9w2dg 146 13 substitution substitution NN cord-255936-hfa9w2dg 146 14 avoids avoid VBZ cord-255936-hfa9w2dg 146 15 deceptive deceptive JJ cord-255936-hfa9w2dg 146 16 imprinting imprinting NN cord-255936-hfa9w2dg 146 17 and and CC cord-255936-hfa9w2dg 146 18 broadens broaden VBZ cord-255936-hfa9w2dg 146 19 the the DT cord-255936-hfa9w2dg 146 20 immune immune JJ cord-255936-hfa9w2dg 146 21 response response NN cord-255936-hfa9w2dg 146 22 to to IN cord-255936-hfa9w2dg 146 23 foot foot NN cord-255936-hfa9w2dg 146 24 - - HYPH cord-255936-hfa9w2dg 146 25 and and CC cord-255936-hfa9w2dg 146 26 - - HYPH cord-255936-hfa9w2dg 146 27 mouth mouth NN cord-255936-hfa9w2dg 146 28 disease disease NN cord-255936-hfa9w2dg 146 29 virus virus NN cord-255936-hfa9w2dg 146 30 Introduction introduction NN cord-255936-hfa9w2dg 146 31 of of IN cord-255936-hfa9w2dg 146 32 neutralizing neutralize VBG cord-255936-hfa9w2dg 146 33 immunogenicity immunogenicity NN cord-255936-hfa9w2dg 146 34 index index NN cord-255936-hfa9w2dg 146 35 to to IN cord-255936-hfa9w2dg 146 36 the the DT cord-255936-hfa9w2dg 146 37 rational rational JJ cord-255936-hfa9w2dg 146 38 design design NN cord-255936-hfa9w2dg 146 39 of of IN cord-255936-hfa9w2dg 146 40 MERS MERS NNP cord-255936-hfa9w2dg 146 41 coronavirus coronavirus NN cord-255936-hfa9w2dg 146 42 subunit subunit NN cord-255936-hfa9w2dg 146 43 vaccines vaccine VBZ cord-255936-hfa9w2dg 146 44 Critical critical JJ cord-255936-hfa9w2dg 146 45 epitopes epitope NNS cord-255936-hfa9w2dg 146 46 in in IN cord-255936-hfa9w2dg 146 47 the the DT cord-255936-hfa9w2dg 146 48 nucleocapsid nucleocapsid NNP cord-255936-hfa9w2dg 146 49 protein protein NN cord-255936-hfa9w2dg 146 50 of of IN cord-255936-hfa9w2dg 146 51 SFTS SFTS NNP cord-255936-hfa9w2dg 146 52 virus virus NN cord-255936-hfa9w2dg 146 53 recognized recognize VBN cord-255936-hfa9w2dg 146 54 by by IN cord-255936-hfa9w2dg 146 55 a a DT cord-255936-hfa9w2dg 146 56 panel panel NN cord-255936-hfa9w2dg 146 57 of of IN cord-255936-hfa9w2dg 146 58 SFTS SFTS NNP cord-255936-hfa9w2dg 146 59 patients patient NNS cord-255936-hfa9w2dg 146 60 derived derive VBD cord-255936-hfa9w2dg 146 61 human human JJ cord-255936-hfa9w2dg 146 62 monoclonal monoclonal JJ cord-255936-hfa9w2dg 146 63 antibodies antibody NNS cord-255936-hfa9w2dg 146 64 Signal Signal NNP cord-255936-hfa9w2dg 146 65 peptide peptide JJ cord-255936-hfa9w2dg 146 66 cleavage cleavage NN cord-255936-hfa9w2dg 146 67 from from IN cord-255936-hfa9w2dg 146 68 GP5 GP5 NNP cord-255936-hfa9w2dg 146 69 of of IN cord-255936-hfa9w2dg 146 70 PRRSV PRRSV NNP cord-255936-hfa9w2dg 147 1 : : : cord-255936-hfa9w2dg 147 2 a a DT cord-255936-hfa9w2dg 147 3 minor minor JJ cord-255936-hfa9w2dg 147 4 fraction fraction NN cord-255936-hfa9w2dg 147 5 of of IN cord-255936-hfa9w2dg 147 6 molecules molecule NNS cord-255936-hfa9w2dg 147 7 retains retain VBZ cord-255936-hfa9w2dg 147 8 the the DT cord-255936-hfa9w2dg 147 9 decoy decoy NN cord-255936-hfa9w2dg 147 10 epitope epitope NN cord-255936-hfa9w2dg 147 11 , , , cord-255936-hfa9w2dg 147 12 a a DT cord-255936-hfa9w2dg 147 13 presumed presume VBN cord-255936-hfa9w2dg 147 14 molecular molecular JJ cord-255936-hfa9w2dg 147 15 cause cause NN cord-255936-hfa9w2dg 147 16 for for IN cord-255936-hfa9w2dg 147 17 viral viral JJ cord-255936-hfa9w2dg 147 18 persistence persistence NN cord-255936-hfa9w2dg 148 1 The the DT cord-255936-hfa9w2dg 148 2 murine murine JJ cord-255936-hfa9w2dg 148 3 gammaherpesvirus-68 gammaherpesvirus-68 CD cord-255936-hfa9w2dg 148 4 gp150 gp150 NN cord-255936-hfa9w2dg 148 5 acts act NNS cord-255936-hfa9w2dg 148 6 as as IN cord-255936-hfa9w2dg 148 7 an an DT cord-255936-hfa9w2dg 148 8 immunogenic immunogenic JJ cord-255936-hfa9w2dg 148 9 decoy decoy NN cord-255936-hfa9w2dg 148 10 to to TO cord-255936-hfa9w2dg 148 11 limit limit VB cord-255936-hfa9w2dg 148 12 virion virion NN cord-255936-hfa9w2dg 148 13 neutralization neutralization NN cord-255936-hfa9w2dg 148 14 Antibody Antibody NNP cord-255936-hfa9w2dg 148 15 recognition recognition NN cord-255936-hfa9w2dg 148 16 of of IN cord-255936-hfa9w2dg 148 17 porcine porcine JJ cord-255936-hfa9w2dg 148 18 circovirus circovirus JJ cord-255936-hfa9w2dg 148 19 type type NN cord-255936-hfa9w2dg 148 20 2 2 CD cord-255936-hfa9w2dg 148 21 capsid capsid NN cord-255936-hfa9w2dg 148 22 protein protein NN cord-255936-hfa9w2dg 148 23 epitopes epitope NNS cord-255936-hfa9w2dg 148 24 after after IN cord-255936-hfa9w2dg 148 25 vaccination vaccination NN cord-255936-hfa9w2dg 148 26 , , , cord-255936-hfa9w2dg 148 27 infection infection NN cord-255936-hfa9w2dg 148 28 , , , cord-255936-hfa9w2dg 148 29 and and CC cord-255936-hfa9w2dg 148 30 disease disease NN cord-255936-hfa9w2dg 148 31 Replacing replace VBG cord-255936-hfa9w2dg 148 32 the the DT cord-255936-hfa9w2dg 148 33 decoy decoy NN cord-255936-hfa9w2dg 148 34 epitope epitope NN cord-255936-hfa9w2dg 148 35 of of IN cord-255936-hfa9w2dg 148 36 PCV2b PCV2b NNP cord-255936-hfa9w2dg 148 37 capsid capsid NN cord-255936-hfa9w2dg 148 38 protein protein NN cord-255936-hfa9w2dg 148 39 with with IN cord-255936-hfa9w2dg 148 40 a a DT cord-255936-hfa9w2dg 148 41 protective protective JJ cord-255936-hfa9w2dg 148 42 epitope epitope NN cord-255936-hfa9w2dg 148 43 enhances enhance VBZ cord-255936-hfa9w2dg 148 44 efficacy efficacy NN cord-255936-hfa9w2dg 148 45 of of IN cord-255936-hfa9w2dg 148 46 PCV2b PCV2b NNP cord-255936-hfa9w2dg 148 47 vaccine vaccine NN cord-255936-hfa9w2dg 148 48 Structural structural JJ cord-255936-hfa9w2dg 148 49 insights insight NNS cord-255936-hfa9w2dg 148 50 into into IN cord-255936-hfa9w2dg 148 51 the the DT cord-255936-hfa9w2dg 148 52 assembly assembly NN cord-255936-hfa9w2dg 148 53 and and CC cord-255936-hfa9w2dg 148 54 regulation regulation NN cord-255936-hfa9w2dg 148 55 of of IN cord-255936-hfa9w2dg 148 56 distinct distinct JJ cord-255936-hfa9w2dg 148 57 viral viral JJ cord-255936-hfa9w2dg 148 58 capsid capsid NN cord-255936-hfa9w2dg 148 59 complexes complex NNS cord-255936-hfa9w2dg 148 60 Interaction Interaction NNP cord-255936-hfa9w2dg 148 61 of of IN cord-255936-hfa9w2dg 148 62 the the DT cord-255936-hfa9w2dg 148 63 replication replication NN cord-255936-hfa9w2dg 148 64 proteins protein NNS cord-255936-hfa9w2dg 148 65 and and CC cord-255936-hfa9w2dg 148 66 the the DT cord-255936-hfa9w2dg 148 67 capsid capsid JJ cord-255936-hfa9w2dg 148 68 protein protein NN cord-255936-hfa9w2dg 148 69 of of IN cord-255936-hfa9w2dg 148 70 porcine porcine JJ cord-255936-hfa9w2dg 148 71 circovirus circovirus NNP cord-255936-hfa9w2dg 148 72 type type NN cord-255936-hfa9w2dg 148 73 1 1 CD cord-255936-hfa9w2dg 148 74 and and CC cord-255936-hfa9w2dg 148 75 2 2 CD cord-255936-hfa9w2dg 148 76 with with IN cord-255936-hfa9w2dg 148 77 host host NN cord-255936-hfa9w2dg 148 78 proteins protein NNS cord-255936-hfa9w2dg 148 79 Recognition Recognition NNP cord-255936-hfa9w2dg 148 80 of of IN cord-255936-hfa9w2dg 148 81 the the DT cord-255936-hfa9w2dg 148 82 different different JJ cord-255936-hfa9w2dg 148 83 structural structural JJ cord-255936-hfa9w2dg 148 84 forms form NNS cord-255936-hfa9w2dg 148 85 of of IN cord-255936-hfa9w2dg 148 86 the the DT cord-255936-hfa9w2dg 148 87 capsid capsid JJ cord-255936-hfa9w2dg 148 88 protein protein NN cord-255936-hfa9w2dg 148 89 determines determine VBZ cord-255936-hfa9w2dg 148 90 the the DT cord-255936-hfa9w2dg 148 91 outcome outcome NN cord-255936-hfa9w2dg 148 92 following follow VBG cord-255936-hfa9w2dg 148 93 infection infection NN cord-255936-hfa9w2dg 148 94 with with IN cord-255936-hfa9w2dg 148 95 porcine porcine JJ cord-255936-hfa9w2dg 148 96 circovirus circovirus JJ cord-255936-hfa9w2dg 148 97 type type NN cord-255936-hfa9w2dg 148 98 2 2 CD cord-255936-hfa9w2dg 148 99 Genetic genetic JJ cord-255936-hfa9w2dg 148 100 variation variation NN cord-255936-hfa9w2dg 148 101 of of IN cord-255936-hfa9w2dg 148 102 porcine porcine JJ cord-255936-hfa9w2dg 148 103 circovirus circovirus JJ cord-255936-hfa9w2dg 148 104 type type NN cord-255936-hfa9w2dg 148 105 2 2 CD cord-255936-hfa9w2dg 148 106 ( ( -LRB- cord-255936-hfa9w2dg 148 107 PCV2 PCV2 NNP cord-255936-hfa9w2dg 148 108 ) ) -RRB- cord-255936-hfa9w2dg 148 109 and and CC cord-255936-hfa9w2dg 148 110 its -PRON- PRP$ cord-255936-hfa9w2dg 148 111 relevance relevance NN cord-255936-hfa9w2dg 148 112 to to IN cord-255936-hfa9w2dg 148 113 vaccination vaccination NN cord-255936-hfa9w2dg 148 114 , , , cord-255936-hfa9w2dg 148 115 pathogenesis pathogenesis NN cord-255936-hfa9w2dg 148 116 and and CC cord-255936-hfa9w2dg 148 117 diagnosis diagnosis NN cord-255936-hfa9w2dg 148 118 Commercial commercial JJ cord-255936-hfa9w2dg 148 119 porcine porcine JJ cord-255936-hfa9w2dg 148 120 circovirus circovirus NN cord-255936-hfa9w2dg 148 121 type type NN cord-255936-hfa9w2dg 148 122 2 2 CD cord-255936-hfa9w2dg 148 123 vaccines vaccine NNS cord-255936-hfa9w2dg 148 124 : : : cord-255936-hfa9w2dg 148 125 efficacy efficacy NN cord-255936-hfa9w2dg 148 126 and and CC cord-255936-hfa9w2dg 148 127 clinical clinical JJ cord-255936-hfa9w2dg 148 128 CP CP NNP cord-255936-hfa9w2dg 148 129 169e180 169e180 CD cord-255936-hfa9w2dg 148 130 epitope epitope NN cord-255936-hfa9w2dg 148 131 level level NN cord-255936-hfa9w2dg 148 132 was be VBD cord-255936-hfa9w2dg 148 133 measured measure VBN cord-255936-hfa9w2dg 148 134 in in IN cord-255936-hfa9w2dg 148 135 an an DT cord-255936-hfa9w2dg 148 136 enzyme enzyme NN cord-255936-hfa9w2dg 148 137 immunoassay immunoassay NN cord-255936-hfa9w2dg 148 138 using use VBG cord-255936-hfa9w2dg 148 139 the the DT cord-255936-hfa9w2dg 148 140 anti anti JJ cord-255936-hfa9w2dg 148 141 - - JJ cord-255936-hfa9w2dg 148 142 CP cp JJ cord-255936-hfa9w2dg 148 143 169e180 169e180 CD cord-255936-hfa9w2dg 148 144 antibody antibody NN cord-255936-hfa9w2dg 148 145 . . . cord-255936-hfa9w2dg 149 1 ( ( -LRB- cord-255936-hfa9w2dg 149 2 C C NNP cord-255936-hfa9w2dg 149 3 ) ) -RRB- cord-255936-hfa9w2dg 149 4 Anti anti JJ cord-255936-hfa9w2dg 149 5 - - JJ cord-255936-hfa9w2dg 149 6 PCV2 pcv2 JJ cord-255936-hfa9w2dg 149 7 antibody antibody NN cord-255936-hfa9w2dg 149 8 in in IN cord-255936-hfa9w2dg 149 9 the the DT cord-255936-hfa9w2dg 149 10 immunized immunize VBN cord-255936-hfa9w2dg 149 11 sera sera NNP cord-255936-hfa9w2dg 149 12 was be VBD cord-255936-hfa9w2dg 149 13 determined determine VBN cord-255936-hfa9w2dg 149 14 by by IN cord-255936-hfa9w2dg 149 15 an an DT cord-255936-hfa9w2dg 149 16 indirect indirect JJ cord-255936-hfa9w2dg 149 17 fluorescence fluorescence NN cord-255936-hfa9w2dg 149 18 assay assay NN cord-255936-hfa9w2dg 149 19 using use VBG cord-255936-hfa9w2dg 149 20 PCV2-infected PCV2-infected NNP cord-255936-hfa9w2dg 149 21 PK-15 PK-15 NNP cord-255936-hfa9w2dg 149 22 cells cell NNS cord-255936-hfa9w2dg 149 23 ( ( -LRB- cord-255936-hfa9w2dg 149 24 n n CC cord-255936-hfa9w2dg 149 25 ¼ ¼ NNP cord-255936-hfa9w2dg 149 26 5 5 CD cord-255936-hfa9w2dg 149 27 / / SYM cord-255936-hfa9w2dg 149 28 group group NN cord-255936-hfa9w2dg 149 29 ) ) -RRB- cord-255936-hfa9w2dg 149 30 . . . cord-255936-hfa9w2dg 150 1 ( ( -LRB- cord-255936-hfa9w2dg 150 2 D d NN cord-255936-hfa9w2dg 150 3 ) ) -RRB- cord-255936-hfa9w2dg 151 1 The the DT cord-255936-hfa9w2dg 151 2 amount amount NN cord-255936-hfa9w2dg 151 3 of of IN cord-255936-hfa9w2dg 151 4 anti anti JJ cord-255936-hfa9w2dg 151 5 - - JJ cord-255936-hfa9w2dg 151 6 CP cp JJ cord-255936-hfa9w2dg 151 7 169e180 169e180 CD cord-255936-hfa9w2dg 151 8 antibody antibody NN cord-255936-hfa9w2dg 151 9 in in IN cord-255936-hfa9w2dg 151 10 the the DT cord-255936-hfa9w2dg 151 11 immunized immunize VBN cord-255936-hfa9w2dg 151 12 sera sera NNP cord-255936-hfa9w2dg 151 13 was be VBD cord-255936-hfa9w2dg 151 14 measured measure VBN cord-255936-hfa9w2dg 151 15 in in IN cord-255936-hfa9w2dg 151 16 an an DT cord-255936-hfa9w2dg 151 17 enzyme enzyme NN cord-255936-hfa9w2dg 151 18 immunoassay immunoassay NN cord-255936-hfa9w2dg 151 19 using use VBG cord-255936-hfa9w2dg 151 20 a a DT cord-255936-hfa9w2dg 151 21 CP CP NNP cord-255936-hfa9w2dg 151 22 169e180 169e180 CD cord-255936-hfa9w2dg 151 23 peptide peptide NN cord-255936-hfa9w2dg 151 24 - - HYPH cord-255936-hfa9w2dg 151 25 coated coated JJ cord-255936-hfa9w2dg 151 26 microtiter microtiter NN cord-255936-hfa9w2dg 151 27 plate plate NN cord-255936-hfa9w2dg 151 28 . . . cord-255936-hfa9w2dg 152 1 ( ( -LRB- cord-255936-hfa9w2dg 152 2 E e NN cord-255936-hfa9w2dg 152 3 ) ) -RRB- cord-255936-hfa9w2dg 152 4 Neutralizing neutralize VBG cord-255936-hfa9w2dg 152 5 activity activity NN cord-255936-hfa9w2dg 152 6 of of IN cord-255936-hfa9w2dg 152 7 the the DT cord-255936-hfa9w2dg 152 8 immunized immunize VBN cord-255936-hfa9w2dg 152 9 sera sera NNP cord-255936-hfa9w2dg 152 10 was be VBD cord-255936-hfa9w2dg 152 11 measured measure VBN cord-255936-hfa9w2dg 152 12 with with IN cord-255936-hfa9w2dg 152 13 a a DT cord-255936-hfa9w2dg 152 14 virus virus NN cord-255936-hfa9w2dg 152 15 neutralization neutralization NN cord-255936-hfa9w2dg 152 16 assay assay NN cord-255936-hfa9w2dg 152 17 . . . cord-255936-hfa9w2dg 153 1 Results result NNS cord-255936-hfa9w2dg 153 2 are be VBP cord-255936-hfa9w2dg 153 3 the the DT cord-255936-hfa9w2dg 153 4 means mean NNS cord-255936-hfa9w2dg 153 5 ± ± NNP cord-255936-hfa9w2dg 153 6 standard standard JJ cord-255936-hfa9w2dg 153 7 deviations deviation NNS cord-255936-hfa9w2dg 153 8 Virus virus NN cord-255936-hfa9w2dg 153 9 - - HYPH cord-255936-hfa9w2dg 153 10 like like JJ cord-255936-hfa9w2dg 153 11 particles particle NNS cord-255936-hfa9w2dg 153 12 in in IN cord-255936-hfa9w2dg 153 13 vaccine vaccine NN cord-255936-hfa9w2dg 153 14 development development NN cord-255936-hfa9w2dg 153 15 Antibody Antibody NNP cord-255936-hfa9w2dg 153 16 responses response NNS cord-255936-hfa9w2dg 153 17 following follow VBG cord-255936-hfa9w2dg 153 18 vaccination vaccination NN cord-255936-hfa9w2dg 153 19 versus versus IN cord-255936-hfa9w2dg 153 20 infection infection NN cord-255936-hfa9w2dg 153 21 in in IN cord-255936-hfa9w2dg 153 22 a a DT cord-255936-hfa9w2dg 153 23 porcine porcine JJ cord-255936-hfa9w2dg 153 24 circovirus circovirus JJ cord-255936-hfa9w2dg 153 25 - - HYPH cord-255936-hfa9w2dg 153 26 type type NN cord-255936-hfa9w2dg 153 27 2 2 CD cord-255936-hfa9w2dg 153 28 ( ( -LRB- cord-255936-hfa9w2dg 153 29 PCV2 PCV2 NNP cord-255936-hfa9w2dg 153 30 ) ) -RRB- cord-255936-hfa9w2dg 153 31 disease disease NN cord-255936-hfa9w2dg 153 32 model model NN cord-255936-hfa9w2dg 153 33 show show VBP cord-255936-hfa9w2dg 153 34 distinct distinct JJ cord-255936-hfa9w2dg 153 35 differences difference NNS cord-255936-hfa9w2dg 153 36 in in IN cord-255936-hfa9w2dg 153 37 virus virus NN cord-255936-hfa9w2dg 153 38 neutralization neutralization NN cord-255936-hfa9w2dg 153 39 and and CC cord-255936-hfa9w2dg 153 40 epitope epitope NN cord-255936-hfa9w2dg 153 41 recognition recognition NN cord-255936-hfa9w2dg 153 42 Efficient efficient JJ cord-255936-hfa9w2dg 153 43 production production NN cord-255936-hfa9w2dg 153 44 of of IN cord-255936-hfa9w2dg 153 45 type type NN cord-255936-hfa9w2dg 153 46 2 2 CD cord-255936-hfa9w2dg 153 47 porcine porcine JJ cord-255936-hfa9w2dg 153 48 circovirus circovirus JJ cord-255936-hfa9w2dg 153 49 - - HYPH cord-255936-hfa9w2dg 153 50 like like JJ cord-255936-hfa9w2dg 153 51 particles particle NNS cord-255936-hfa9w2dg 153 52 by by IN cord-255936-hfa9w2dg 153 53 a a DT cord-255936-hfa9w2dg 153 54 recombinant recombinant JJ cord-255936-hfa9w2dg 153 55 baculovirus baculovirus NN cord-255936-hfa9w2dg 153 56 Characterization characterization NN cord-255936-hfa9w2dg 153 57 of of IN cord-255936-hfa9w2dg 153 58 porcine porcine JJ cord-255936-hfa9w2dg 153 59 circovirus circovirus JJ cord-255936-hfa9w2dg 153 60 type type NN cord-255936-hfa9w2dg 153 61 2 2 CD cord-255936-hfa9w2dg 153 62 ( ( -LRB- cord-255936-hfa9w2dg 153 63 PCV2 PCV2 NNP cord-255936-hfa9w2dg 153 64 ) ) -RRB- cord-255936-hfa9w2dg 153 65 capsid capsid JJ cord-255936-hfa9w2dg 153 66 particle particle NN cord-255936-hfa9w2dg 153 67 assembly assembly NN cord-255936-hfa9w2dg 153 68 and and CC cord-255936-hfa9w2dg 153 69 its -PRON- PRP$ cord-255936-hfa9w2dg 153 70 application application NN cord-255936-hfa9w2dg 153 71 to to IN cord-255936-hfa9w2dg 153 72 virus virus NN cord-255936-hfa9w2dg 153 73 - - HYPH cord-255936-hfa9w2dg 153 74 like like JJ cord-255936-hfa9w2dg 153 75 particle particle NN cord-255936-hfa9w2dg 153 76 vaccine vaccine NN cord-255936-hfa9w2dg 153 77 development development NN cord-255936-hfa9w2dg 153 78 Expression expression NN cord-255936-hfa9w2dg 153 79 of of IN cord-255936-hfa9w2dg 153 80 porcine porcine JJ cord-255936-hfa9w2dg 153 81 circovirus circovirus NNP cord-255936-hfa9w2dg 153 82 2 2 CD cord-255936-hfa9w2dg 153 83 ORF2 ORF2 NNP cord-255936-hfa9w2dg 153 84 gene gene NN cord-255936-hfa9w2dg 153 85 requires require VBZ cord-255936-hfa9w2dg 153 86 codon codon NN cord-255936-hfa9w2dg 153 87 optimized optimize VBN cord-255936-hfa9w2dg 153 88 E. E. NNP cord-255936-hfa9w2dg 153 89 coli coli NNS cord-255936-hfa9w2dg 153 90 cells cell NNS cord-255936-hfa9w2dg 153 91 Barbas Barbas NNP cord-255936-hfa9w2dg 153 92 3rd 3rd NN cord-255936-hfa9w2dg 153 93 , , , cord-255936-hfa9w2dg 153 94 Methods Methods NNPS cord-255936-hfa9w2dg 153 95 for for IN cord-255936-hfa9w2dg 153 96 the the DT cord-255936-hfa9w2dg 153 97 generation generation NN cord-255936-hfa9w2dg 153 98 of of IN cord-255936-hfa9w2dg 153 99 chicken chicken NN cord-255936-hfa9w2dg 153 100 monoclonal monoclonal JJ cord-255936-hfa9w2dg 153 101 antibody antibody NN cord-255936-hfa9w2dg 153 102 fragments fragment NNS cord-255936-hfa9w2dg 153 103 by by IN cord-255936-hfa9w2dg 153 104 phage phage NN cord-255936-hfa9w2dg 153 105 display display NN cord-255936-hfa9w2dg 153 106 Phage phage NN cord-255936-hfa9w2dg 153 107 Display display NN cord-255936-hfa9w2dg 153 108 : : : cord-255936-hfa9w2dg 153 109 a a DT cord-255936-hfa9w2dg 153 110 Laboratory Laboratory NNP cord-255936-hfa9w2dg 153 111 Manual Manual NNP cord-255936-hfa9w2dg 154 1 A a DT cord-255936-hfa9w2dg 154 2 point point NN cord-255936-hfa9w2dg 154 3 mutation mutation NN cord-255936-hfa9w2dg 154 4 in in IN cord-255936-hfa9w2dg 154 5 the the DT cord-255936-hfa9w2dg 154 6 heavy heavy JJ cord-255936-hfa9w2dg 154 7 chain chain NN cord-255936-hfa9w2dg 154 8 complementarity complementarity NN cord-255936-hfa9w2dg 154 9 - - HYPH cord-255936-hfa9w2dg 154 10 determining determine VBG cord-255936-hfa9w2dg 154 11 region region NN cord-255936-hfa9w2dg 154 12 3 3 CD cord-255936-hfa9w2dg 154 13 ( ( -LRB- cord-255936-hfa9w2dg 154 14 HCDR3 HCDR3 NNP cord-255936-hfa9w2dg 154 15 ) ) -RRB- cord-255936-hfa9w2dg 154 16 significantly significantly RB cord-255936-hfa9w2dg 154 17 enhances enhance VBZ cord-255936-hfa9w2dg 154 18 the the DT cord-255936-hfa9w2dg 154 19 specificity specificity NN cord-255936-hfa9w2dg 154 20 of of IN cord-255936-hfa9w2dg 154 21 an an DT cord-255936-hfa9w2dg 154 22 anti anti JJ cord-255936-hfa9w2dg 154 23 - - JJ cord-255936-hfa9w2dg 154 24 ROS1 ros1 JJ cord-255936-hfa9w2dg 154 25 antibody antibody NN cord-255936-hfa9w2dg 154 26 An an DT cord-255936-hfa9w2dg 154 27 antibody antibody NN cord-255936-hfa9w2dg 154 28 reactive reactive JJ cord-255936-hfa9w2dg 154 29 to to IN cord-255936-hfa9w2dg 154 30 the the DT cord-255936-hfa9w2dg 154 31 Gly63-Lys68 Gly63-Lys68 NNP cord-255936-hfa9w2dg 154 32 epitope epitope NN cord-255936-hfa9w2dg 154 33 of of IN cord-255936-hfa9w2dg 154 34 NT NT NNP cord-255936-hfa9w2dg 154 35 - - HYPH cord-255936-hfa9w2dg 154 36 proBNP proBNP NNP cord-255936-hfa9w2dg 154 37 exhibits exhibit VBZ cord-255936-hfa9w2dg 154 38 O o NN cord-255936-hfa9w2dg 154 39 - - HYPH cord-255936-hfa9w2dg 154 40 glycosylation glycosylation NN cord-255936-hfa9w2dg 154 41 - - HYPH cord-255936-hfa9w2dg 154 42 independent independent JJ cord-255936-hfa9w2dg 154 43 binding bind VBG cord-255936-hfa9w2dg 154 44 Chicken Chicken NNP cord-255936-hfa9w2dg 154 45 scFvs scFvs NNP cord-255936-hfa9w2dg 154 46 with with IN cord-255936-hfa9w2dg 154 47 an an DT cord-255936-hfa9w2dg 154 48 artificial artificial JJ cord-255936-hfa9w2dg 154 49 cysteine cysteine NN cord-255936-hfa9w2dg 154 50 for for IN cord-255936-hfa9w2dg 154 51 site site NN cord-255936-hfa9w2dg 154 52 - - HYPH cord-255936-hfa9w2dg 154 53 directed direct VBN cord-255936-hfa9w2dg 154 54 conjugation conjugation NN cord-255936-hfa9w2dg 154 55 Western western JJ cord-255936-hfa9w2dg 154 56 blotting blotting NN cord-255936-hfa9w2dg 154 57 " " '' cord-255936-hfa9w2dg 154 58 : : : cord-255936-hfa9w2dg 154 59 electrophoretic electrophoretic JJ cord-255936-hfa9w2dg 154 60 transfer transfer NN cord-255936-hfa9w2dg 154 61 of of IN cord-255936-hfa9w2dg 154 62 proteins protein NNS cord-255936-hfa9w2dg 154 63 from from IN cord-255936-hfa9w2dg 154 64 sodium sodium NN cord-255936-hfa9w2dg 154 65 dodecyl dodecyl NNP cord-255936-hfa9w2dg 154 66 sulfateepolyacrylamide sulfateepolyacrylamide NN cord-255936-hfa9w2dg 154 67 gels gel NNS cord-255936-hfa9w2dg 154 68 to to IN cord-255936-hfa9w2dg 154 69 unmodified unmodified JJ cord-255936-hfa9w2dg 154 70 nitrocellulose nitrocellulose NN cord-255936-hfa9w2dg 154 71 and and CC cord-255936-hfa9w2dg 154 72 radiographic radiographic JJ cord-255936-hfa9w2dg 154 73 detection detection NN cord-255936-hfa9w2dg 154 74 with with IN cord-255936-hfa9w2dg 154 75 antibody antibody NN cord-255936-hfa9w2dg 154 76 and and CC cord-255936-hfa9w2dg 154 77 radioiodinated radioiodinated JJ cord-255936-hfa9w2dg 154 78 protein protein NN cord-255936-hfa9w2dg 155 1 A a DT cord-255936-hfa9w2dg 155 2 Evaluation evaluation NN cord-255936-hfa9w2dg 155 3 of of IN cord-255936-hfa9w2dg 155 4 commercial commercial JJ cord-255936-hfa9w2dg 155 5 polyclonal polyclonal JJ cord-255936-hfa9w2dg 155 6 - - HYPH cord-255936-hfa9w2dg 155 7 and and CC cord-255936-hfa9w2dg 155 8 monoclonal monoclonal JJ cord-255936-hfa9w2dg 155 9 - - HYPH cord-255936-hfa9w2dg 155 10 antibody antibody NN cord-255936-hfa9w2dg 155 11 - - HYPH cord-255936-hfa9w2dg 155 12 based base VBN cord-255936-hfa9w2dg 155 13 immunohistochemical immunohistochemical JJ cord-255936-hfa9w2dg 155 14 tests test NNS cord-255936-hfa9w2dg 155 15 for for IN cord-255936-hfa9w2dg 155 16 2 2 CD cord-255936-hfa9w2dg 155 17 genotypes genotype NNS cord-255936-hfa9w2dg 155 18 of of IN cord-255936-hfa9w2dg 155 19 Porcine porcine JJ cord-255936-hfa9w2dg 155 20 circovirus circovirus JJ cord-255936-hfa9w2dg 155 21 type type NN cord-255936-hfa9w2dg 155 22 2 2 CD cord-255936-hfa9w2dg 155 23 and and CC cord-255936-hfa9w2dg 155 24 comparison comparison NN cord-255936-hfa9w2dg 155 25 with with IN cord-255936-hfa9w2dg 155 26 in in IN cord-255936-hfa9w2dg 155 27 - - HYPH cord-255936-hfa9w2dg 155 28 situ situ NN cord-255936-hfa9w2dg 155 29 hybridization hybridization NN cord-255936-hfa9w2dg 155 30 assays assay NNS cord-255936-hfa9w2dg 155 31 Comparative comparative JJ cord-255936-hfa9w2dg 155 32 efficacy efficacy NN cord-255936-hfa9w2dg 155 33 of of IN cord-255936-hfa9w2dg 155 34 experimental experimental JJ cord-255936-hfa9w2dg 155 35 inactivated inactivate VBN cord-255936-hfa9w2dg 155 36 and and CC cord-255936-hfa9w2dg 155 37 live live RB cord-255936-hfa9w2dg 155 38 - - HYPH cord-255936-hfa9w2dg 155 39 attenuated attenuated JJ cord-255936-hfa9w2dg 155 40 chimeric chimeric JJ cord-255936-hfa9w2dg 155 41 porcine porcine JJ cord-255936-hfa9w2dg 155 42 circovirus circovirus NN cord-255936-hfa9w2dg 155 43 ( ( -LRB- cord-255936-hfa9w2dg 155 44 PCV pcv NN cord-255936-hfa9w2dg 155 45 ) ) -RRB- cord-255936-hfa9w2dg 155 46 1 1 CD cord-255936-hfa9w2dg 155 47 - - HYPH cord-255936-hfa9w2dg 155 48 2b 2b CD cord-255936-hfa9w2dg 155 49 vaccines vaccine NNS cord-255936-hfa9w2dg 155 50 derived derive VBN cord-255936-hfa9w2dg 155 51 from from IN cord-255936-hfa9w2dg 155 52 PCV1 PCV1 NNP cord-255936-hfa9w2dg 155 53 and and CC cord-255936-hfa9w2dg 155 54 PCV2b PCV2b NNP cord-255936-hfa9w2dg 155 55 isolates isolate NNS cord-255936-hfa9w2dg 155 56 originated originate VBD cord-255936-hfa9w2dg 155 57 in in IN cord-255936-hfa9w2dg 155 58 China China NNP cord-255936-hfa9w2dg 155 59 Production Production NNP cord-255936-hfa9w2dg 155 60 , , , cord-255936-hfa9w2dg 155 61 preliminary preliminary JJ cord-255936-hfa9w2dg 155 62 characterisation characterisation NN cord-255936-hfa9w2dg 155 63 and and CC cord-255936-hfa9w2dg 155 64 applications application NNS cord-255936-hfa9w2dg 155 65 of of IN cord-255936-hfa9w2dg 155 66 monoclonal monoclonal JJ cord-255936-hfa9w2dg 155 67 antibodies antibody NNS cord-255936-hfa9w2dg 155 68 to to IN cord-255936-hfa9w2dg 155 69 porcine porcine JJ cord-255936-hfa9w2dg 155 70 circovirus circovirus JJ cord-255936-hfa9w2dg 155 71 Characterization characterization NN cord-255936-hfa9w2dg 155 72 of of IN cord-255936-hfa9w2dg 155 73 immune immune JJ cord-255936-hfa9w2dg 155 74 response response NN cord-255936-hfa9w2dg 155 75 of of IN cord-255936-hfa9w2dg 155 76 young young JJ cord-255936-hfa9w2dg 155 77 pigs pig NNS cord-255936-hfa9w2dg 155 78 to to IN cord-255936-hfa9w2dg 155 79 porcine porcine JJ cord-255936-hfa9w2dg 155 80 circovirus circovirus JJ cord-255936-hfa9w2dg 155 81 type type NN cord-255936-hfa9w2dg 155 82 2 2 CD cord-255936-hfa9w2dg 155 83 infection infection NN cord-255936-hfa9w2dg 155 84 Global global JJ cord-255936-hfa9w2dg 155 85 status status NN cord-255936-hfa9w2dg 155 86 of of IN cord-255936-hfa9w2dg 155 87 porcine porcine JJ cord-255936-hfa9w2dg 155 88 circovirus circovirus JJ cord-255936-hfa9w2dg 155 89 type type NN cord-255936-hfa9w2dg 155 90 2 2 CD cord-255936-hfa9w2dg 155 91 and and CC cord-255936-hfa9w2dg 155 92 its -PRON- PRP$ cord-255936-hfa9w2dg 155 93 associated associated JJ cord-255936-hfa9w2dg 155 94 diseases disease NNS cord-255936-hfa9w2dg 155 95 in in IN cord-255936-hfa9w2dg 155 96 sub sub JJ cord-255936-hfa9w2dg 155 97 - - JJ cord-255936-hfa9w2dg 155 98 saharan saharan JJ cord-255936-hfa9w2dg 155 99 africa africa NNP cord-255936-hfa9w2dg 155 100 Enhanced enhanced JJ cord-255936-hfa9w2dg 155 101 production production NN cord-255936-hfa9w2dg 155 102 of of IN cord-255936-hfa9w2dg 155 103 porcine porcine JJ cord-255936-hfa9w2dg 155 104 circovirus circovirus JJ cord-255936-hfa9w2dg 155 105 type type NN cord-255936-hfa9w2dg 155 106 2 2 CD cord-255936-hfa9w2dg 155 107 ( ( -LRB- cord-255936-hfa9w2dg 155 108 PCV2 PCV2 NNP cord-255936-hfa9w2dg 155 109 ) ) -RRB- cord-255936-hfa9w2dg 155 110 virus virus NN cord-255936-hfa9w2dg 155 111 - - HYPH cord-255936-hfa9w2dg 155 112 like like JJ cord-255936-hfa9w2dg 155 113 particles particle NNS cord-255936-hfa9w2dg 155 114 in in IN cord-255936-hfa9w2dg 155 115 Sf9 sf9 NN cord-255936-hfa9w2dg 155 116 cells cell NNS cord-255936-hfa9w2dg 155 117 by by IN cord-255936-hfa9w2dg 155 118 translational translational JJ cord-255936-hfa9w2dg 155 119 enhancers enhancer NNS cord-255936-hfa9w2dg 155 120 Protection Protection NNP cord-255936-hfa9w2dg 155 121 of of IN cord-255936-hfa9w2dg 155 122 swine swine NN cord-255936-hfa9w2dg 155 123 against against IN cord-255936-hfa9w2dg 155 124 post post JJ cord-255936-hfa9w2dg 155 125 - - JJ cord-255936-hfa9w2dg 155 126 weaning wean VBG cord-255936-hfa9w2dg 155 127 multisystemic multisystemic NNP cord-255936-hfa9w2dg 155 128 wasting waste VBG cord-255936-hfa9w2dg 155 129 syndrome syndrome NN cord-255936-hfa9w2dg 155 130 ( ( -LRB- cord-255936-hfa9w2dg 155 131 PMWS PMWS NNP cord-255936-hfa9w2dg 155 132 ) ) -RRB- cord-255936-hfa9w2dg 155 133 by by IN cord-255936-hfa9w2dg 155 134 porcine porcine JJ cord-255936-hfa9w2dg 155 135 circovirus circovirus NNP cord-255936-hfa9w2dg 155 136 type type NN cord-255936-hfa9w2dg 155 137 2 2 CD cord-255936-hfa9w2dg 156 1 ( ( -LRB- cord-255936-hfa9w2dg 156 2 PCV2 PCV2 NNP cord-255936-hfa9w2dg 156 3 ) ) -RRB- cord-255936-hfa9w2dg 156 4 proteins protein NNS cord-255936-hfa9w2dg 156 5 Immunogenicity Immunogenicity NNP cord-255936-hfa9w2dg 156 6 of of IN cord-255936-hfa9w2dg 156 7 empty empty JJ cord-255936-hfa9w2dg 156 8 capsids capsid NNS cord-255936-hfa9w2dg 156 9 of of IN cord-255936-hfa9w2dg 156 10 porcine porcine JJ cord-255936-hfa9w2dg 156 11 circovius circovius NN cord-255936-hfa9w2dg 156 12 type type NN cord-255936-hfa9w2dg 156 13 2 2 CD cord-255936-hfa9w2dg 156 14 produced produce VBN cord-255936-hfa9w2dg 156 15 in in IN cord-255936-hfa9w2dg 156 16 insect insect NN cord-255936-hfa9w2dg 156 17 cells cell NNS cord-255936-hfa9w2dg 156 18 Immunity immunity NN cord-255936-hfa9w2dg 156 19 conferred confer VBN cord-255936-hfa9w2dg 156 20 by by IN cord-255936-hfa9w2dg 156 21 an an DT cord-255936-hfa9w2dg 156 22 experimental experimental JJ cord-255936-hfa9w2dg 156 23 vaccine vaccine NN cord-255936-hfa9w2dg 156 24 based base VBN cord-255936-hfa9w2dg 156 25 on on IN cord-255936-hfa9w2dg 156 26 the the DT cord-255936-hfa9w2dg 156 27 recombinant recombinant JJ cord-255936-hfa9w2dg 156 28 PCV2 PCV2 NNP cord-255936-hfa9w2dg 156 29 Cap Cap NNP cord-255936-hfa9w2dg 156 30 protein protein NN cord-255936-hfa9w2dg 156 31 expressed express VBN cord-255936-hfa9w2dg 156 32 in in IN cord-255936-hfa9w2dg 156 33 Trichoplusia Trichoplusia NNP cord-255936-hfa9w2dg 156 34 ni ni NNP cord-255936-hfa9w2dg 156 35 - - HYPH cord-255936-hfa9w2dg 156 36 larvae larva NNS cord-255936-hfa9w2dg 156 37 Predicting predict VBG cord-255936-hfa9w2dg 156 38 flexible flexible JJ cord-255936-hfa9w2dg 156 39 length length NN cord-255936-hfa9w2dg 156 40 linear linear JJ cord-255936-hfa9w2dg 156 41 B b NN cord-255936-hfa9w2dg 156 42 - - HYPH cord-255936-hfa9w2dg 156 43 cell cell NN cord-255936-hfa9w2dg 156 44 epitopes epitope NNS cord-255936-hfa9w2dg 156 45 We -PRON- PRP cord-255936-hfa9w2dg 156 46 would would MD cord-255936-hfa9w2dg 156 47 like like VB cord-255936-hfa9w2dg 156 48 to to TO cord-255936-hfa9w2dg 156 49 thank thank VB cord-255936-hfa9w2dg 156 50 Tae Tae NNP cord-255936-hfa9w2dg 156 51 - - HYPH cord-255936-hfa9w2dg 156 52 Eun Eun NNP cord-255936-hfa9w2dg 156 53 Kim Kim NNP cord-255936-hfa9w2dg 156 54 for for IN cord-255936-hfa9w2dg 156 55 the the DT cord-255936-hfa9w2dg 156 56 technical technical JJ cord-255936-hfa9w2dg 156 57 assistance assistance NN cord-255936-hfa9w2dg 156 58 and and CC cord-255936-hfa9w2dg 156 59 Taeyeub Taeyeub NNP cord-255936-hfa9w2dg 156 60 Lee Lee NNP cord-255936-hfa9w2dg 156 61 for for IN cord-255936-hfa9w2dg 156 62 structural structural JJ cord-255936-hfa9w2dg 156 63 modeling modeling NN cord-255936-hfa9w2dg 156 64 of of IN cord-255936-hfa9w2dg 156 65 PCV2 PCV2 NNP cord-255936-hfa9w2dg 156 66 using use VBG cord-255936-hfa9w2dg 156 67 PyMOL1.3 PyMOL1.3 NNP cord-255936-hfa9w2dg 156 68 . . . cord-255936-hfa9w2dg 156 69 _SP cord-255936-hfa9w2dg 157 1 Transparency transparency NN cord-255936-hfa9w2dg 157 2 document document NN cord-255936-hfa9w2dg 157 3 related relate VBN cord-255936-hfa9w2dg 157 4 to to IN cord-255936-hfa9w2dg 157 5 this this DT cord-255936-hfa9w2dg 157 6 article article NN cord-255936-hfa9w2dg 157 7 can can MD cord-255936-hfa9w2dg 157 8 be be VB cord-255936-hfa9w2dg 157 9 found find VBN cord-255936-hfa9w2dg 157 10 online online RB cord-255936-hfa9w2dg 157 11 at at IN cord-255936-hfa9w2dg 157 12 https://doi.org/10.1016/j.bbrc.2018.01.141 https://doi.org/10.1016/j.bbrc.2018.01.141 CD cord-255936-hfa9w2dg 157 13 . . .