id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326613-253v48i0	Lv, Dandan	A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries	2020-05-29		.txt	text/plain	1083	59	47	title: A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries Previously, the excessive recruitment and activation of neutrophils (polymorphonuclear leukocytes [PMNs]), accompanied by neutrophil extracellular traps (NETs) formation were reported being implicated in the pathogenesis of ALI/ARDS. Therefore, we established a cell-based assay for detecting NETs during lung epithelial cells-neutrophils co-culture using the xCELLigence system, a recognized real-time, dynamic, label-free, sensitive, and high-throughput apparatus. Our results demonstrated that lung epithelial injuries, reflected by declines in cell index (CI) values, could be induced by lipopolysaccharide (LPS)-activated PMNs, or NETs in a time and dose-dependent manner. Moreover, this cell-based assay identified that PMNs from severe pneumonia patients had a high NETs formative potential. Protein-based therapies for acute lung injury: targeting neutrophil extracellular traps Neutrophil extracellular traps directly induce epithelial and endothelial 669 cell death: a predominant role of histones Maladaptive role of neutrophil extracellular traps in 671 pathogen-induced lung injury	./cache/cord-326613-253v48i0.txt	./txt/cord-326613-253v48i0.txt