key: cord-271485-a1633xxe
authors: Leaf, Rebecca Karp; Al‐Samkari, Hanny; Brenner, Samantha K.; Gupta, Shruti; Leaf, David E.
title: ABO Phenotype and Death in Critically Ill Patients with COVID‐19
date: 2020-07-01
journal: Br J Haematol
DOI: 10.1111/bjh.16984
sha: 
doc_id: 271485
cord_uid: a1633xxe

Blood groups are inherited traits that vary across populations, likely due to both founder effects and natural selection. A link between blood groups and susceptibility to infectious disease has been well‐described, with notable examples being H. Pylori and Plasmodium falciparum infection. Blood group antigens may influence disease susceptibility by several mechanisms, including serving as receptors or decoys for infectious organisms and modifying immune response in the form of anti‐ABO antibodies.

Blood groups are inherited traits that vary across populations, likely due to both founder effects and natural selection. 1 A link between blood groups and susceptibility to infectious disease has been welldescribed, with notable examples being H. Pylori and Plasmodium falciparum infection. 1, 2 Blood group antigens may influence disease susceptibility by several mechanisms, including serving as receptors or decoys for infectious organisms and modifying immune response in the form of anti-ABO antibodies. 2 Data on the relationship between blood group and outcomes in patients with coronavirus disease 2019 (COVID-19) are limited. Studies from China 3 and Europe 4 reported that patients with type O blood may be protected from COVID-19 infection, whereas those with type A blood may be at higher risk. Data from a related viral outbreak, the severe acute respiratory syndrome coronavirus (SARS-CoV-1) in 2003, suggested that healthcare workers with type O blood were less likely to contract this disease. 5 In vitro experiments revealed that the interaction between the SARS-CoV-1 spike protein and angiotensin converting enzyme 2 (ACE2)-necessary for viral uptake-may be mitigated by anti-A antibodies. 6 To examine the relationship between blood group and clinical outcomes in patients with COVID-19, we studied the distribution and mortality associated with ABO phenotype in a large cohort of critically ill patients. We utilized data from the Study of the Treatment and Outcomes in critically ill Patients with COVID-19 (STOP-COVID), a multicenter cohort study that enrolled consecutive adults (≥18years-old) with laboratory-confirmed COVID-19 admitted to participating intensive care units (ICUs) at 67 hospitals across the United States. We included patients admitted to ICUs between March 4 and April 11, 2020. We followed patients until the first of hospital discharge, death, or May 8, 2020the date on which the database for the current analysis was locked. All patients who remained hospitalized at the time of analysis had a minimum of 28 days follow-up. The study was approved with a waiver of informed consent by the Institutional Review Board at each participating site.

To examine whether blood type is associated with critical illness in patients with COVID-19, we used a chi-square test to compare the observed versus expected distribution of ABO phenotypes. We stratified our analyses by race/ethnicity, since race/ethnicity are important determinants of ABO phenotype 7 and could also affect hospitalization for COVID-19. To improve the reliability of our estimates, we limited our analyses to the three most commonly reported categories of race/ethnicity Accepted Article in our cohort: White non-Hispanic; Black non-Hispanic; and Hispanic. Patients missing data on ABO phenotype were excluded. We estimated the expected distribution of ABO phenotype in each of the above race/ethnicity categories using data from 3.1 million blood donors in the United States. 7 To examine whether ABO phenotype is associated with mortality among critically ill patients with COVID-19, we used a chi-square test to compare the distribution of observed ABO blood phenotypes with 28-day in-hospital mortality, stratified by the above race/ethnicity categories. Patients discharged alive from the hospital prior to 28 days were considered to be alive at 28 days. We tested the validity of this assumption in a random subset of 50 patients discharged prior to 28 days, all of whom remained alive at 28 days according to electronic medical records or follow-up by phone. Among 3239 critically ill patients with COVID-19, 2033 (62.8%) had data available on ABO phenotype and were included in the current analysis. The median age was 62 years (interquartile range, 52-71) and 1297 (63.8%) were men. Additional characteristics according to ABO phenotype are shown in Table 1 .

The observed and expected frequencies of ABO phenotypes in White, Black, and Hispanic patients are shown in Figure 1 . Among White patients, the observed distribution of ABO phenotypes differed from its expected distribution ( Figure 1A) . This difference was primarily driven by patients with blood type A being over-represented (45.1% observed versus 39.8% expected) and patients with blood type O being under-represented (37.8% observed versus 45.2% expected). Among Black ( Figure   1B ) and Hispanic patients ( Figure 1C ) the observed and expected distributions of ABO phenotypes were similar.

A total of 799 of the 2033 patients (39.3%) died within 28 days. The mortality rate was similar across ABO phenotypes in all race/ethnicity categories (Figures 1D-F) . Results were qualitatively unchanged when considering Rh phenotype.

In this large nationwide cohort study of critically ill patients with COVID-19, we found significant differences in the observed versus expected distribution of ABO phenotypes among White patients,

with blood types A and O being over-and under-represented, respectively. We found no difference in the observed versus expected distribution of ABO phenotypes among Black or Hispanic patients, nor did we find an association between ABO phenotype and 28-day mortality among any of the three examined categories of race/ethnicity. Our finding of a higher than expected frequency of blood type A and a lower than expected frequency of blood type O-at least among White patients-is consistent with other reports. For instance, in a genome wide association study of nearly 2,000 patients in Italy and Spain, Ellinghaus and colleagues recently identified two gene clusters enriched in patients with COVID-19. One cluster contained genes relevant to both ACE2 functionality and immune response, while the other cluster encoded genes for the ABO blood group. In a meta-analysis of two different case-control cohorts, the authors found that type A blood conferred a higher risk of severe COVID-19 while type O blood afforded protection. 4 Similarly, investigators from both China and the United States reported that patients with type A blood are at increased risk of developing COVID-19 whereas those with type O blood have a lower risk. 3 The mechanisms responsible for these observations have yet to be elucidated. One theory is that neutralizing anti-A antibodies protect against viral entry into lung epithelium, as was hypothesized with SARS-CoV-1; 6 however, if this were the case, we would have expected both type O and type B blood to be under-represented in our cohort. Alternatively, individuals with type O blood are known to have lower rates of thrombosis and cardiovascular disease, which is attributed to altered glycosyltransferase activity and increased clearance of von Willebrand factor. Thus, patients with type O blood may be less likely to develop COVID-19-related microvascular thrombosis and endothelial dysfunction. 8 We are unable to provide a discrete biological explanation as to why we found a difference in the incidence of COVID-19-associated critical illness only among White patients, though this finding is consistent with prior reports demonstrating a higher risk of acute respiratory distress syndrome in White patients with type A blood but not in Black patients. 9 We would also note that there is large variation, subjectivity, and bias in how race/ethnicity is reported in the US, and that these results should be interpreted with caution. 10

This article is protected by copyright. All rights reserved Our study has several strengths. We used granular data from a large number of consecutive critically ill patients from 67 geographically-diverse sites across the US. Further, whereas prior studies in COVID-19 have had limited follow-up, we followed patients until hospital discharge, death, or a minimum of 28 days.

We also acknowledge several limitations. As with all observational analyses, we cannot rule out the possibility of residual confounding. Additionally, data on ABO phenotype were missing in approximately one-third of patients. Finally, we were unable to evaluate other blood groups, such as secretor status and Lewis antigens, which are also known to affect host immunity. 1, 2 In conclusion, our data suggest that type A blood may be a risk factor for COVID-19-related critical illness among White patients, and that type O blood may be protective. Future investigations are needed to determine the mechanisms for these findings.

This article is protected by copyright. All rights reserved 

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