id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-297567-38t82q9t	Lamarre, Alain	A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice	2005-12-06		.txt	text/plain	3702	180	52	Purified scFv fragments retained the antigen‐binding properties of the parental antibody, could inhibit its binding to viral antigens with apparently higher efficiency than monovalent antigen‐binding (Fab) fragments, but neutralized viral infectivity with lower efficiency (about sevenfold at a molar level). Therefore, even though the scFv could neutralize viral infectivity in vitro, the same quantity of fragments that partially protected mice in the form of Fab only slightly delayed virus‐induced lethality when injected as scFv fragments, probably because of a much faster in vivo clearance: the biologic half‐life was estimated to be about 6 min. To explore the possibility of using antibody fragments expressed in bacteria for the treatment of viral diseases, an scFv was constructed from the sequences of MHV-specific mAb 7-1 OA and its in vitro neutralization and in vivo protection properties were evaluated. The neutralization capacity of the recombinant scFv fragment was evaluated and compared to that of the Fab fragment by incubating 50 PFU of virus with dilutions of purified fragments and determining the residual viral infectivity on murine fibroblast cells (Fig. 6) .	./cache/cord-297567-38t82q9t.txt	./txt/cord-297567-38t82q9t.txt