id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321667-jkzxjk54	Papineau, Amber	Genome Organization of Canada Goose Coronavirus, A Novel Species Identified in a Mass Die-off of Canada Geese	2019-04-11		.txt	text/plain	3491	189	57	Comparative genomics and phylogenetic analysis indicate it is a new species of Gammacoronavirus, as it falls below the threshold of 90% amino acid similarity in the protein domains used to demarcate Coronaviridae. Additional features that distinguish the genome of Canada goose coronavirus include 6 novel ORFs, a partial duplication of the 4 gene and a presumptive change in the proteolytic processing of polyproteins 1a and 1ab. As the full genome was sequenced from only the cloacal swab of a single Canada goose, a screening PCR was designed based on the 4b duplication region unique to CGCoV and performed on all samples. To summarize, the complete genome of CGCoV, a novel Gammacoronavirus species was sequenced directly from the cloacal swab of a Canada goose associated with a mass die-off. The CGCoV genome was also detected in samples derived from a second Canada goose and a snow goose that perished in the die-off, using PCR, Sanger and high throughput sequencing.	./cache/cord-321667-jkzxjk54.txt	./txt/cord-321667-jkzxjk54.txt