key: cord-313258-luiw46zj
authors: Kim, Joon Hwan; Choi, Ji-Yeon; Kim, Na Yeon; Kim, Jin Woo; Baek, Ji Hyeon; Baek, Hye Sung; Yoon, Jung Won; Jee, Hye Mi; Choi, Sun Hee; Kim, Hyeung Yoon; Kim, Ki Eun; Shin, Youn Ho; Han, Man Yong
title: Clinical risk factors associated with the development of wheezing in children less than 2 years of age who required hospitalization for viral lower respiratory tract infections
date: 2015-07-22
journal: Korean J Pediatr
DOI: 10.3345/kjp.2015.58.7.245
sha: 
doc_id: 313258
cord_uid: luiw46zj

PURPOSE: Wheezing following viral lower respiratory tract infections (LRTIs) in children <2 years of age is an important risk factor for the development of asthma later in life; however, not all children with viral LRTIs develop wheezing. This study investigated risk factors for the development of wheezing during viral LRTIs requiring hospitalization. METHODS: The study included 142 children <2 years of age hospitalized for LRTIs with at least one virus identified as the cause and classified them into children diagnosed with LRTIs with wheezing (n=70) and those diagnosed with LRTIs without wheezing (n=72). RESULTS: There were no significant differences in the viruses detected between the two groups. Multivariate logistic regression analysis showed that, after adjusting for potentially confounding variables including sex and age, the development of wheezing was strongly associated with parental history of allergic diseases (adjusted odds ratio [aOR], 20.19; 95% confidence interval [CI], 3.22-126.48), past history of allergic diseases (aOR, 13.95; 95% CI, 1.34-145.06), past history of hospitalization for respiratory illnesses (aOR, 21.36; 95% CI, 3.77-120.88), exposure to secondhand smoke at home (aOR, 14.45; 95% CI, 4.74-44.07), and total eosinophil count (aOR, 1.01; 95% CI, 1.01-1.02). CONCLUSION: Past and parental history of allergic diseases, past history of hospitalization for respiratory illnesses, exposure to secondhand smoke at home, and total eosinophil count were closely associated with the development of wheezing in children <2 years of age who required hospitalization for viral LRTIs. Clinicians should take these factors into consideration when treating, counseling, and monitoring young children admitted for viral LRTIs.

Lower respiratory tract infections (LRTIs) are common in early childhood and are mostly caused by respiratory viruses. Wheezing caused by viruses is considered an important risk factor for the development of asthma later in life [1] [2] [3] [4] [5] .

Although the same virus may cause LRTIs, only those who develop wheezing during LRTIs are more likely to develop asthma 1, 5) . Thus, upon infection with a virus, children less than 2 years of age with and without wheezing may differ with regard to the development of recurrent wheezing and/or asthma. Children diagnosed with LRTIs with wheezing would be more likely to develop recurrent wheezing and/or asthma in late childhood than those with LRTIs without wheezing, even though they are infected with the same virus. In this regard, previous studies have reported risk factors for young children who are more likely to have wheezing during or following a viral LRTI 6, 7) . However, there are relatively small data focusing on the clinical risk factors related to the development of wheezing in young children with LRTIs who require hospitalization.

This study was designed to determine clinical risk factors associated with the development of wheezing in infants and young children less than 2 years of age who require hospitali zation for viral LRTIs.

We conducted a cross-sectional study of all infants and children admitted to the CHA Bundang Medical Center from August 2006 through July 2007 with signs and symptoms of LRTIs. Inclusion criteria were as follows: (1) children less than 2 years of age; and (2) children with a positive nasopharyngeal washing aspirate confirming infection with at least one of the 12 respi ratory viruses. A total of 400 children were admitted with LRTIs during the study period. All had signs and symptoms of LRTIs: i.e., cough, tachypnea, respiratory distress, and breathing difficulties with grunting or wheezing sounds with rales 8) . After a complete physical examination by an experienced pediatrician, children who developed wheezing on auscultation at admission and/or during hospitalization were defined as the LRTIs with wheezing and those who did not develop wheezing were defined as the LRTIs without wheezing.

Infants and young children were excluded if (1) they were infected with Mycoplasma pneumoniae, defined as an initial indirect microparticle agglutinin method (Serodia-Myco II, Fujirebio, Japan) (titer of >1:320) or a fourfold rising titer in double-checking samples and positivity for anti-Mycoplasma IgM 9) , (2) no virus was detected, (3) their parents or guardians did not respond to questionnaires regarding the patient's past and parental history at admission, (4) they were born prematurely (i.e., less than 37 weeks gestational age), or (5) they had a cardiac anomaly. Clinical factors recorded at admission included sex; age; the presence of fever; laboratory findings; patient's and parental history of allergic diseases, including asthma 10) , allergic rhinitislike symptoms 11) , and atopic dermatitis 12) ; previous hospitalization for respiratory illnesses; and smoking by any member of the house hold during the previous year, with the latter defined as exposure of the patient to secondhand smoke. At admission, blood samples were obtained from the antecubital vein of each subject and laboratory tests, including complete blood count, absolute neutrophil count, total eosinophil count, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR), were performed and recorded for each subject. The study protocol was reviewed and approved by the Institutional Review Board of CHA University Medical Center, Seongnam, Korea, and written informed consent was obtained from the parents/guardians of each patient.

Nasopharyngeal aspirates were obtained upon admission by gently flushing the children's nostrils with 4 mL of sterile saline solution. All nasopharyngeal aspirates were tested within 24 hours of collection for respiratory syncytial viruses (A and B), human metapneumovirus, human rhinovirus, influenza A and B viruses, parainfluenza (A, B, and C) virus, coronavirus (A and B), and adenovirus. Total viral RNA was extracted from nasopharyngeal aspirates (300 μL) using Viral Gene-spin Viral DNA/RNA Extraction Kits (iNtRON, Seongnam, Korea) and stored at -80°C. First-strand cDNA was synthesized using Revert Aid First Strand cDNA Synthesis Kits (Fermentas Inc., Burlington, ON, Canada), followed by polymerase chain reactions using Seeplex Respiratory Viruses Detection Kits-1 (Seegene, Seoul, South Korea) and the GeneAmp PCR system 9700 (Applied Biosystems, Waltham, MA, USA). Each 20 μL reaction mixture included 3 μL of cDNA, 4 μL of 5× RV1A or 5× RV1B primer, and 10 μL of 2× Multiplex Master Mix. Briefly, 2.5 μL of extracted RNA was mixed with 5× buffer, 0.2mM of each dNTP, 0.5μM of each primer [13] [14] [15] [16] , 1 μL of enzyme mix, and DEPC-treated ultrapure water to a final volume of 25 μL. After incubations at 50°C for 30 minutes and at 94°C for 15 minutes, the reactions were subjected to 40 cycles of denaturation for 30 seconds at 94°C, annealing for 90 seconds at 60°C, and extension for 90 seconds at 72°C, followed by a final extension at 72°C for 10 minutes. The ampli fied products were analyzed on 2% agarose gels containing 0.5 g/mL ethidium bromide.

Data are expressed as mean±standard deviation unless otherwise indicated. Between-group differences were compared using independent Student t tests, Mann-Whitney U tests, or chi-square tests, as appropriate. Multivariate logistic regression analysis was used to identify clinical risk factors for the development of wheez ing caused by viruses, with adjusted odds ratios (aORs) and 95% confidence intervals (CIs) calculated; age and total eosinophil count were used as continuous variables and the other factors were used as categorical variables. All statistical analyses were performed using IBM SPSS Statistics ver. 21.0 (IBM Co., Korean J Pediatr 2015;58 (7):245-250

Armonk, NY, USA). All statistical tests were two-sided, with a P value of <0.05 indicating statistical significance.

The demographic and clinical characteristics of the study population are shown in Table 1 . The study population consisted of 142 children aged <2 years, 70 children with LRTIs with wheezing and 72 children with LRTIs without wheezing (Fig. 1) . The mean ages of the children with the LRTIs with and without wheezing were 7.0±5.4 and 8.3±5.9 months, respectively. Approximately 70% of the children in each group were male. There were no between-group differences in sex, age, absolute neutro phil count, CRP concentration, and ESR. 

The children with the LRTIs with wheezing were more likely to have parents with a history of allergic diseases than the children with the LRTIs without wheezing ( to secondhand smoke at home (aOR, 14.45; 95% CI, 4.74-44.07), and total eosinophil count (aOR, 1.01; 95% CI, 1.01-1.02) ( Table 3 ).

The present study showed that past and parental history of allergic diseases, past history of hospitalization for respiratory illnesses, exposure to secondhand smoke at home, and serum total eosinophil count were independently associated with the development of wheezing in children <2 years of age who had viral lower respiratory infections requiring hospitalization.

Our findings are in good agreement with those of previous studies, which reported that the development of childhood wheezing is closely associated with parental history of allergic disease 17) and a personal history of allergic diseases 18) . A recent study from the PARIS (Pollution and Asthma Risk: an Infant Study) birth cohort focusing on the determinants of wheezing severity in infants found that the risk of severe wheezing was mainly ascribed to the atopic status of the child 17) . This study corresponds well with our findings that the children included in the present study had signs and symptoms severe enough to require hospitalization and that the children diagnosed with LRTIs with wheezing had a higher incidence of parental and past history of allergic diseases than those with LRTIs without wheezing. Viral lower respiratory infection is a major risk factor for development of wheezing. These illnesses are thought to interact with allergic inflammation, altering airways by damaging lower epithelial cells, increasing the release of mast cell mediators, and amplifying allergic inflammation 19) . Several viruses, including respiratory syncytial virus [20] [21] [22] , human rhinovirus 20, 23, 24) , and human metapneumovirus 20, 25) , have been associated with the development of wheezing in children. In contrast, we observed no differences in the incidence of each virus in the children diagnos-cantly more likely to have past history of allergic diseases than those diagnosed with the LRTIs without wheezing ( Table 2 shows the comparison of viruses identified in the study subjects diagnosed with LRTIs with and without wheezing. Respiratory syncytial virus was detected in 39 subjects (55.7%) in the children diagnosed with LRTIs with wheezing and 37 (51.4%) in those diagnosed with LRTIs without wheezing; human metapneu movirus in 14 (20.0%) and 9 (12.5%), respectively; and human rhinovirus in 12 (17.1%) and 11 (15.3%), respectively. There were no between-group differences in the incidence of each virus (P>0.05 each).

We utilized multivariate analysis to determine the relationships between the development of wheezing and clinical variables in the study subjects. Multivariate logistic regression analysis showed that after adjusting for potential confounding variables including sex and age, the development of wheezing was strongly associated with parental history of allergic diseases (aOR, 20.19; 95% CI, 3.22-126.48), past history of allergic diseases (aOR, 13.95; 95% CI, 1.34-145.06), past history of hospitalization for respiratory illnesses (aOR, 21.36; 95% CI, 3.77-120.88), exposure Korean J Pediatr 2015;58 (7):245-250 ed with and without wheezing, suggesting that, at least in this cohort of children, the development of wheezing was not associated with specific viral agents causing LRTIs requiring hospitalization.

Previous studies have suggested the hypothesis that susceptibility to viral bronchiolitis may be an early manifestation of biased immune responses, which associates with both diminished viral defense and atopic airway inflammation 26) . Furthermore, viral infection in infancy may alter subsequent Th1/Th2 immune responses and enhance Th2 sensitization to inhalant allergens, resulting in the development of an asthmatic phenotype 27, 28) . Although we did not look into the long-term outcome of the children with LRTIs with wheezing, we speculate that the development of wheezing in these young children may be an early manifestation of Th2-biased immune response to viral respiratory infections. Therefore, the interaction between age at initial infection, genetic susceptibility, and virus will determine the outcome of respiratory virus infection and airway disorders.

One clinically important finding in the present study is that the children diagnosed with LRTIs with wheezing showed a lower incidence of temperature >38℃, a lower WBC count, and a higher total eosinophil count when compared with those with LRTIs without wheezing. This may be due to the notion that upon viral LRTI, the children with wheezing may demonstrate Th2-type response, whereas those without wheezing demonstrate Th1-type response; this is also supported by the higher incidence of past and parental history of allergic diseases in children with wheezing. It is well-known that blood eosinophil count reflects systemic eosinophilic inflammation and associates with having wheeze. A recent cross-sectional study examining the value of blood eosinophil count in a random population sample of 12,408 subjects found that a higher blood eosinophil count was closely associated with having asthma, wheeze, and asthma attacks 29) , a finding that corresponds well with our results.

The harmful effects of exposure to secondhand smoke on the development of wheezing in infants and toddlers are well established 17, [30] [31] [32] [33] . In line with previous studies, we found that exposure to secondhand smoke was a risk factor for development of wheezing in children with viral LRTIs requiring hospitalization. The severity of bronchiolitis in early life has been shown to be altered by postnatal exposure to maternal tobacco smoke, and by atopy and age of the infant 34) . Thus, children exposed to secondhand smoke are at risk of airway infiltration by small particles, making these children more susceptible to develop wheezing when infected with a respiratory virus. We speculate that contrary to the other clinical risk factors, exposure to secondhand smoke at home is a modifiable environmental risk factor for young children in terms of preventing the development of wheezing when inflicted with viral LRTIs.

The present study had some limitations. First, it was of cross-sectional design; therefore, we were unable to determine predictive factors for recurrent wheezing in late childhood. Second, we did not classify familial smoking into smoking during pregnancy and/or after delivery or into paternal and/or maternal smoking. Third, the infants were not subjected to pulmonary function tests; therefore, wheezing and lung function were not measured in an objective manner. Fourth, the present study yielded high odds ratios for clinical factors such as past and parental history of allergic diseases with regard to the development of wheeze in the study subjects. This may be ascribed to the fact that they are the most likely sensitizers to wheezing in children hospitalized for LRTIs. Finally, we did not determine allergic sensitization of the study subjects, which is considered to play a role in the development of wheezing 35) .

In conclusion, we found that past and parental history of allergic diseases, past history of hospitalization for respiratory illnesses, exposure to secondhand smoke at home, and total eosinophil count were closely associated with the development of wheezing in children less than 2 years of age who required hospitalization for viral LRTIs. Clinicians should take these clinical factors into consideration in treating, counseling, and monitoring young children hospitalized for viral LRTIs.

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No potential conflict of interest relevant to this article was reported.