id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282618-tjvjlyn9	Luke, J M	Improved antibiotic-free plasmid vector design by incorporation of transient expression enhancers	2010-11-25		.txt	text/plain	6241	336	43	To maintain a low risk of insertional mutagenesis-mediated gene activation, expression-augmenting sequences would ideally function to improve transgene expression from transiently transfected intact plasmid, but not from spurious genomically integrated vectors. A neomycin resistance gene (NeoR) without an upstream Kozak sequence was cloned downstream of an enhanced green fluorescent protein (EGFP) transgene in different configurations similar to that used with PREs. Quantifiable neoR translation products were present in all tested configurations, as was biologically active neoR protein after plasmid transfection into both HEK293 and CHO cell lines (Supplementary Table S1 ). The assay was in the same format as in (a), except for the fact that Pol III inhibitor-treated cells were transfected with EGFP plasmids containing the CMV-HTLV-I R promoter with or without VA1; (c) Inhibition of PKR, not of adenosine deaminase acting on RNA (ADAR) or RNA interference (RNAI), was required for VA1 expression enhancement effect.	./cache/cord-282618-tjvjlyn9.txt	./txt/cord-282618-tjvjlyn9.txt