key: cord-335692-5uxtua9o authors: Kilic, A.; Elliott, S.; Hester, L.; Palavecino, E. title: Evaluation of the performance of DiaSorin molecular Pneumocystis jirovecii-CMV multiplex real-time PCR assay from bronchoalveolar lavage samples date: 2020-01-31 journal: J Mycol Med DOI: 10.1016/j.mycmed.2020.100936 sha: doc_id: 335692 cord_uid: 5uxtua9o The aim of this study was to evaluate the performance of the DiaSorin Molecular PJ-CMV multiplex real-time PCR (PJ-CMV PCR) assay (DiaSorin Molecular LLC, USA) in bronchoalveolar lavage (BAL) samples compared to direct immunofluorescence assay (IFA) for the detection of Pneumocystis jirovecii and assess CMV and P. jirovecii co-infection rate in immunosuppressed patients with suspected pneumonia. A total of 125 BAL samples from immunosuppressed patients submitted for PJP-IFA were tested. Surplus samples were saved and further tested by using the PJ-CMV PCR assay. Among the 125 samples, P. jirovecii was detected in 31.2% (39/125) and in 40% (50/125) of the specimens using IFA and PJ-CMV PCR respectively. Eleven of the PJ-CMV PCR positive samples were negative by direct IFA for P. jirovecii. All samples positive by direct IFA were also positive by PJ-CMV PCR. Using the direct IFA as a gold standard, the PJ-CMV PCR sensitivity, specificity, positive predictive value and negative predictive value for detection of P. jirovecii were 100%, 87.2%, 78% and 100%, respectively. However, after reviewing the clinical diagnosis, the specificity and PPV increased to 100%. Of the 50 P. jirovecii samples positive by PJ-CMV PCR, 18 (36%) were also positive for CMV by the PJ-CMV PCR. The co-infection rate was found to be 37.5% (6/16) and 35.2% (12/34) in HIV infected and non-HIV infected patients. This study indicated that the DiaSorin Molecular PJ-CMV multiplex real-time PCR assay has higher sensitivity than direct IFA for detection of P. jirovecii and provides rapid detection of PJ and CMV infection in BAL samples. The aim of this study was to evaluate the performance of the DiaSorin Molecular PJ-CMV multiplex realtime PCR (PJ-CMV PCR) assay (DiaSorin Molecular LLC, USA) in bronchoalveolar lavage (BAL) samples compared to direct immunofluorescence assay (IFA) for the detection of Pneumocystis jirovecii and assess CMV and P. jirovecii co-infection rate in immunosuppressed patients with suspected pneumonia. A total of 125 BAL samples from immunosuppressed patients submitted for PJP-IFA were tested. Surplus samples were saved and further tested by using the PJ-CMV PCR assay. Among the 125 samples, P. jirovecii was detected in 31.2% (39/125) and in 40% (50/125) of the specimens using IFA and PJ-CMV PCR respectively. Eleven of the PJ-CMV PCR positive samples were negative by direct IFA for P. jirovecii. All samples positive by direct IFA were also positive by PJ-CMV PCR. Using the direct IFA as a gold standard, the PJ-CMV PCR sensitivity, specificity, positive predictive value and negative predictive value for detection of P. jirovecii were 100%, 87.2%, 78% and 100%, respectively. However, after reviewing the clinical diagnosis, the specificity and PPV increased to 100%. Of the 50 P. jirovecii samples positive by PJ-CMV PCR, 18 (36%) were also positive for CMV by the PJ-CMV PCR. The co-infection rate was found to be 37.5% (6/16) and 35.2% (12/34) in HIV infected and non-HIV infected patients. This study indicated that the DiaSorin Molecular PJ-CMV multiplex real-time PCR assay has higher sensitivity than direct IFA for detection of P. jirovecii and provides rapid detection of PJ and CMV infection in BAL samples. C 2020 Elsevier Masson SAS. All rights reserved. clinical suspicion of PJP were tested for P. jirovecii by direct IFA using the Monofluo P. jirovecii IFA test kit (MONOFLUO TM Bio-Rad, Laboratories, USA) according to the manufacturer's instructions. According to the clinical data reviewed, all patients evaluated were considered immunosuppressed and BAL samples were submitted for testing because patients met clinical and radiographic criteria for possible PJP and other infections had been ruled out. The non-HIV infected patients included patients with hemato- . Data collection and analysis were performed with LIAISON 1 MDX Studio software. The following cycling conditions were used: 1 cycle at 978C for 120 s followed by 40 cycles of 978C for 10 s with a ramp speed of 28C/s, and 608C for 30 s with a ramp speed of 28C/s with capture mode on. The test time of the PJ-CMV PCR assay from sample processing to final result is approximately 60 minutes. Extraction and amplification controls were used to detect PCR failure and/or inhibition. Positive and negative control samples were included in each run. Both CMV and PJ positive molecular controls were purchased from Exact Diagnostics (Exact Diagnostics, Fort Worth, TX). Standard procedures were used to obtain the analytical sensitivity of the test for the detection of P. jirovecii and CMV. Briefly, commercially available controls (CMV AD 169-Zeptometrix, Buffalo, NY, and P. jirovecii PCPP100-Exact Diagnostics, Fort Worth, TX) were chosen for the limit of detection (LOD) determination. Serial dilutions were prepared in a pooled BAL negative matrix ranging from 5000 copies/mL to 500 copies/mL for CMV and from 2500 copies/mL to 1000 copies/mL for P. jirovecii. LOD was defined as the minimum concentration with a detection rate of at least 95% by Probit Analyses. For the analytical specificity assessment, the manufacturer tested a panel of 31 LOD was determined to be 2,063 AE 46 copies/mL or 103 copies/ Rxn for CMV and 1,826 AE 44 copies/mL or 91 copies/Rxn for P. jiroveci in the current study. The assay yielded negative results for the panel of microorganisms other than the target organisms and thus no crossreactivity was demonstrated. The results for the clinical samples showed that P. jirovecii was detected in 31.2% (39/125) and 40% (50/ 125) of the specimens using IFA and PJ-CMV PCR, respectively. When we compared the results obtained from HIV-infected and non-HIV infected patients, 16 (45.7%) of the 35 HIV infected and 34 (37.7%) of 90 non-HIV infected patients were positive for P. jirovecii by PJ-CMV PCR. Eleven of the samples positive by PJ-CMV PCR were negative by IFA. All 39 samples positive by IFA were also positive by PCR. There were no samples that were negative by PJ-CMV PCR and positive by IFA. The Ct for detection of PJ (number of cycles needed for a positive result) obtained with the PJ-CMV PCR was higher than 25 cycles (in 7 samples higher than 30) in the 11 samples negative by IFA suggesting that the PJ organism load was lower in the PJ-CMV PCR positive and IFA negative samples. Using IFA as a gold standard, the PJ-CMV PCR sensitivity, specificity, positive predictive value and negative predictive value for P. jirovecii were 100%, 87.2%, 78% and 100%, respectively (Table 1) . Of the 50 P. jirovecii positive samples, 18 (36%) were also positive for CMV by the PJ-CMV PCR. The co-infection rate was found to be 37.5% (6/16) and 35.2% (12/34) in HIV infected and non-HIV infected patients with immunosuppressed conditions, respectively. There are several methods for detection of P. jirovecii from respiratory clinical samples including cytologic examination and IFA. Direct IFA test has been widely used and is still in first line for PJP diagnostic in association with pathogen DNA detection. These tests detect both the cystic and trophic forms of P. jirovecii. Although the direct IFA tests are more rapid to perform and easier to interpret than cytochemical stains, they might have false-negative results, especially in specimens from non-HIV infected patients since these tend to have fewer organisms present in the specimens [8, 9] . These tests also depend upon the skills and experience of the observer in terms of familiarity with the different morphologies of the organisms [10] . An overall 55.5% sensitivity and 98.6% specificity of direct IFA tests has been demonstrated in a meta-analysis article evaluating seven prospective studies [11] . Molecular techniques such as real-time PCR have been used in the diagnostic evaluation of PJP, which have better sensitivity than traditional cytologic stains and IFA [3, 4] . In the present study, we evaluated the performance of the PJ-CMV PCR assay on stored BAL samples against IFA for diagnosis of PJP. To our knowledge, this is the first evaluation of the PJ-CMV PCR assay using reagents from DiaSorin Molecular. The PJ-CMV PCR showed a higher sensitivity and specificity at diagnosing PJP in both HIV-infected and non-HIV infected immunocompromised patients compared to the direct IFA test. The higher sensitivity observed in this study is consistent with the results of previous studies on the detection of P. jirovecii [2, 12, 13] . A bivariate metaanalysis study by Lu et al. presented the diagnostic accuracy of P. jirovecii PCR techniques, with sensitivity ranging from 96% to 100% and specificity ranging from 87% to 93% [12] . In another review, reported sensitivities ranged from 82% to 100% and specificities ranged between 83% and 100% for PJP diagnosis using PCR assays [13] . A bivariate meta-analysis and systemic review by Table 1 Comparison of the P. jirovecii by direct immunofluorescence (IFA) and the PJ-CMV multiplex real-time PCR assay results (N = 125). Negative Total [2] . Previous studies that have compared the performance of realtime PCR to IFA have also reported that a small number of samples were positive by real-time PCR but negative by IFA testing due to the higher sensitivity of the PCR [12, 14, 15] . In this study, eleven samples found positive by PCR were negative by IFA. All 11 of these with potentially false positive results compared to IFA had clinically proven PJP with clinical symptoms. These specimens had an overall higher cycle threshold value compared to samples positive by both methods, which became positive at less than 25 cycles. The specificity increased from 87.2 to 100% and the PPV increased from 78 to 100% once these eleven results were considered true-positive according to patients' clinical diagnostic criteria. One of the advantages of the PJ-CMV PCR assay is that it is designed as a closed system to reduce the potential carry-over contamination from run-to-run with amplicons causing falsepositive results. Clinical samples are not open simultaneously during any step in the process. PJ-CMV PCR assay can be also used on other respiratory fluids like sputum or bronchial fluid. CMV infection has immunomodulatory actions and aggravates the state of immunosuppression. It might lead to higher risk of opportunistic infections with other pathogens such as PJ and significantly influence on the outcome of the PJP infections [16, 17] . However, some studies have reported no significant effect of CMV co-infection on the outcome of PJP [5, 7, 18] . Although the clinical significance of concomitant CMV infection with PJP is poorly understood, some investigators advise that CMV should be monitored closely in PJP infected patients [5] . The PJ-CMV PCR assay detects both of these pathogens in a single sample, eliminating the need for a separate assay for CMV PCR testing in respiratory specimens. Previous studies have reported that 23.4-61.5% of PJP patients also exhibit co-infection with CMV [7, 17, 19, 20] . The percentage of CMV co-infection of this study was found to be 36% of patients, which is consistent with previous studies. There was no significant difference between the HIV infected and non-HIV infected patients in terms of the CMV co-infection. There are a few limitations to this study. Our initial study question restricted the scope of this study and the data that we had access to. In particular, we compared the PJ-CMV PCR assay against direct IFA, the test routinely used at our laboratory, but not against another molecular test or against a quantitative test that could provide a specific organism load. In addition, although all samples analyzed were from patients meeting clinical criteria of PJP, we do not have further clinical data on the patients. In summary, our findings demonstrate that the PJ-CMV multiplex real-time PCR assay showed higher sensitivity compared with direct IFA for detection of P. jirovecii in BAL samples and might be easily adapted for use in the clinical microbiology laboratories allowing the simultaneous detection of PJ and CMV in a single sample. The authors declare that they have no competing interest. 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