id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299051-5r2s8z1a	Tsuhako, Maria Heloisa	Tempol ameliorates murine viral encephalomyelitis by preserving the blood–brain barrier, reducing viral load, and lessening inflammation	2010-03-01		.txt	text/plain	5935	308	44	Furthermore, treatment with tempol decreased CNS viral titers, macrophage and T lymphocyte infiltration, and levels of markers of inflammation, such as expression of inducible nitric oxide synthase, transcription of tumor necrosis factor-α and interferon-γ, and protein nitration. Immunohistochemical analysis of the brain (Fig. 6 ) and spinal cord (data not shown) tissues showed that viral infection resulted in macrophage infiltration, iNOS expression, and protein nitration, all of which were greatly attenuated by tempol treatment. Semiquantitative image analysis of the immunostaining of the brain tissues revealed that treatment with tempol reduced macrophage infiltration, iNOS expression, and 3nitrotyrosine levels to about 4, 26, and 16% of those of untreated mice, respectively (Fig. 7A) . In comparison, tempol is more effective than uric acid in scavenging nitric oxide-derived oxidants [9] , displays low toxicity in animal models [15, 16] , permeates the blood-brain barrier (Fig. 2) , and inhibits the enzyme myeloperoxidase ( [20] ; see Augusto et al., [53] , which has been recently associated with MS [54, 55] .	./cache/cord-299051-5r2s8z1a.txt	./txt/cord-299051-5r2s8z1a.txt