id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338235-vz2d2x18	Pinschewer, Daniel D.	T cells can mediate viral clearance from ependyma but not from brain parenchyma in a major histocompatibility class I- and perforin-independent manner	2010-03-30		.txt	text/plain	7277	349	45	This indicated that it was indeed the absence of perforin-dependent cytolytic pathways rather than a general impairment of CD8 + T cell Virus clearance from ependyma To gain further insights on how rLCMV/INDG persisted in the CNS of perforin-deficient, TCRb À/À and RAG À/À mice but not in C57BL/6 controls, we performed histological time course analyses of viral antigen and of infiltrating CD8 + T cells. However, C57BL/6 wild-type animals eliminated the virus by Day 8, whereas slowly but steadily increasing numbers of infected cells were found within the CNS parenchyma of perforin-deficient, TCRb À/À and RAG À/À mice on Days 8, 10 Frequencies of NP396-specific CD8 + T cells in blood were determined on Day 8 using MHC class I tetramers. Total viral RNA loads in the brain of perforin-deficient, MHCI À/À , TCRb À/À and RAG À/À mice are similar as determined by quantitative RT-PCR (Fig. 6C) , providing evidence that within the brain parenchyma, adaptive immune pathways other than MHCI-and perforin-dependent T cell clearance do not substantially influence rLCMV/INDG persistence.	./cache/cord-338235-vz2d2x18.txt	./txt/cord-338235-vz2d2x18.txt