id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284944-hcgfe9wv	Silvin, Aymeric	Elevated calprotectin and abnormal myeloid cell subsets discriminate severe from mild COVID-19	2020-08-05		.txt	text/plain	10781	503	46	Thus, we performed high dimensional flow cytometry and single cell RNA sequencing of COVID-19 patient peripheral blood cells and detected the disappearance of non-classical CD14LowCD16High monocytes, the accumulation of HLA-DRLow classical monocytes, and the release of massive amounts of calprotectin (S100A8/S100A9) in severe cases. Validating these discovery experiments, we performed mass cytometry analysis of an independent cohort of 12 patients (four in each group; control, mild and severe) ( Table S5) , which showed a lower fraction of CD14 Low CD16 High non-classical monocytes in severe compared to mild patients ( Figure 3F and 3G ). This study presents evidence that patients who develop a severe COVID-19 exhibit high levels of calprotectin and inflammatory cytokines and chemokines correlating with an emergency myelopoiesis generating ROS-and NOS-expressing immunosuppressive myeloid cells (HLA-DR Low monocytes and immature subsets of neutrophils).	./cache/cord-284944-hcgfe9wv.txt	./txt/cord-284944-hcgfe9wv.txt