id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286560-lhbp4nn0	Eckermann, Marina	3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography	2020-08-20		.txt	text/plain	7505	378	48	We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. Overview and regions-of-interest (ROI) scans were recorded on the same paraffinembedded sample, covering a maximum tissue cross section of 8 mm by stitching different tomograms, and with a minimum voxel size of 167 nm in certain ROIs. Scale-bridging and dynamic ROI selection in close spatial and temporal proximity was implemented with dedicated instrumentation the GINIX endstation of the beamline P10/PETRA III (DESY, Hamburg) (Salditt et al., 2015) .	./cache/cord-286560-lhbp4nn0.txt	./txt/cord-286560-lhbp4nn0.txt