id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-288493-i15rb8d2	Rother, N.	Hydroxychloroquine inhibits trained immunity - implications for COVID-19	2020-06-09		.txt	text/plain	7272	474	55	Similar to what we observed at the time of admission, we found enhanced transcription of interferon-stimulated genes five days after admission to be associated with poor outcome (Fig. 3i,j) Taken together, these immune profiling data show that the inflammatory response in SARS-CoV-2 infection is characterized by marked alterations in the innate immune system, a result that corroborates previous reports 14 . To investigate the effect of chloroquine (CQ) and hydroxychloroquine (HCQ) on trained immunity, we adapted a previously described in vitro protocol in which human PBMCs are stimulated with RPMI (control) or HKCA for 24 hours 21 . This effect was abrogated when cells were treated with chloroquine and hydroxychloroquine for 24 hours during HKCA stimulation, indicating that these compounds prevent the induction of trained immunity (Fig. 4b, Extended Data Fig. 4b) . Since interferons play a central role in viral immune responses, and our monocyte transcriptome data from COVID-19 patients revealed enhanced interferon-stimulated gene expression, we investigated how inflammatory monocytes respond to restimulation with IFN.	./cache/cord-288493-i15rb8d2.txt	./txt/cord-288493-i15rb8d2.txt